Bioassays of 

Tampa Electric Company- Bayside 

(formerly Tampa Electric Company – F. J. Gannon Station) 

Hillsborough County 

NPDES #FL0000809 

Sampled June 27, 2005 

September 2005

Biology Section 

Bureau of Laboratories 

Division of Resource Assessment and Management

Quality Manual No. 870346G 

NELAC Certification No. E31780 
Tampa Electric Company - Bayside, 3602 Port Sutton Rd., Tampa, Hillsborough County, Florida, 

NPDES #FL0000809. Effluent samples for this facility were collected on June 27, 2005. 

Introduction 

The Tampa Electric Company Bayside facility (formerly known as Tampa Electric Company - F. J. 

Gannon Station) is a natural gas-fired electric power generation facility. The facility has a design 

flow of 1,346.3 million gallons per day (MGD). The mean flow from June 2004 through May 2005 

was 560 MGD. The flow during the sampling event was 600.5 MGD. Effluent from the facility is 

routed through a common discharge flume (Outfall D-001) and is composed primarily of oncethrough cooling water from Hillsborough Bay. Minor components of the effluent are recycle tank

blow-down and reverse osmosis (RO) concentrate blow-down. Effluent is not treated prior to 

discharge though outfall D-001 into the Class III marine waters of Hillsborough Bay. 

There are no Administrative Orders or Consent Orders concerning this facility. The facility has no 

mixing zones. There have been several violations of flow within the past year. A revision (dated

March 5, 2003) was made to the permit describing changes to discharges from internal outfalls, I037, and I-040. These changes were associated with the re-powering of the plant as a natural 

gas facility instead of a coal-fired plant (facility information provided Lori Pillsbury of FDEP 

Southwest District). 

Methods 

Samples were collected from the D-001 NPDES sampling point by Lori Pillsbury and Nahir 

Cornwell (FDEP Southwest District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. 

All DEP SOP’s are available on the web at: http://www.floridadep.org/labs/qa/sops.htm. 

The 48-hour acute screening toxicity tests and algal growth potential test were performed 

following internal DEP SOP’s TA07_03, TA07_04, and TA08_01 through TA08_07. All internal

DEP SOP’s are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. 

While the 48-hour acute screening toxicity test does not reflect the permit required conditions, the 

Department uses it to provide reasonable assurance that the facility does not adversely affect 

waters of the state. 

The control water for these tests was prepared by adding sufficient natural seawater (salinity of 

40 parts per thousand (ppth)) to aged artificial seawater (salinity of 20 ppth) to produce a solution 

of 23.1 ppth salinity. The salinity of the effluent sample (23.3 ppth) was not adjusted. 

Toxicity Test Results 

Tests were performed June 28 - 30, 2005. 

Americamysis bahia 48-hr acute screening bioassay – LC50 > 100%, 0% mortality* in 100% 

sample at 48 hours 

Menidia beryllina 48-hr acute screening bioassay – LC50 > 100%, 0% mortality* in 100% 

sample at 48 hours 

The bioassay samples were not acutely toxic to the test organisms.

See Appendix 1 for bioassay bench sheets. 

*NOTE: In a 48-hr acute screening test of 100% effluent, mortality of <20% provides reasonable 

assurance that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality

Standards (Rule 62-302.500 (1)(a)4, F.A.C.). Mortality between 20-50% mortality indicates low to

2
moderate levels of toxicity, and further action may be required. Mortality of >50% provides

reasonable assurance that the effluent fails to meet the minimum requirement to discharge to 

waters of the state (Rule 62-4.244(3)(a), F.A.C.) 

Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was 1.07 mg dry wt/L of Dunaliella tertiolecta. Raschke 

and Shultz found that AGP values above 10.0 mg dry weight/L represent a “problem” threshold

for marine receiving waters, implying nutrient enrichment (personal communication with Ron 

Raschke USEPA Region 4 1987). The analytical chemistry suggests that the effluent is nitrogenlimited. 

See Table 1 for AGP results. 

Chemistry Results 

Total residual chlorine and total ammonia were not detected in the bioassay sample in the 

laboratory. The total ammonia concentration in the sample collected and preserved for chemical 

analysis was 0.038 mg/L. Based on the pH, salinity, and temperature of the effluent as collected, 

the calculated unionized ammonia concentration was < 0.02 mg/L.

Aluminum was detected in the effluent at levels that comply with the Class III marine water quality 

criterion. Iron and selenium were detected between the laboratory minimum detection limits 

(MDL) and practical quantitation limits (PQL). 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. 

See Appendix 2 for a complete list of chemical analyses performed.

Conclusion 

The effluent sample collected from Outfall D-001 for this facility on June 27, 2005, was not 

acutely toxic to either test species during the acute screening 48-hour bioassays. The effluent 

AGP result was less than the "problem” threshold for marine receiving waters. Effluent water 

quality samples did not exceed any applicable water quality standards or violate any permit 

conditions. 

Literature Cited 

Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: 

Bottle Test. National Environmental Research Center Office of Research and 

Development. U. S. EPA, Corvallis, Oregon. 43 p. 

3
Location AGP Inorganic N:P

ratio

Total N:P ratio

Effluent Samples 1.07 0.16 2.32

Table 1. Algal growth potential (AGP; mg dry wt/L) of Dunaliella tertiolecta and 

nitrogen to phosphorus ratios for samples collected from Tampa Electric

Company Bayside facility on June 27, 2005.

4
TECO Bayside NPDES# FL0000809 Class III 

Marine Stds

Effluent 

Limits

Effluent 

Samples

Aluminum ≤ 1500 - 89

Arsenic ≤ 50 - 8 U

Cadmium ≤ 9.3 - 0.5 U

Calcium (mg/L) - - 271

Chromium-III - - 9.4 U

Copper ≤ 3.7 ≤ 3.7 5 U

Iron ≤ 300 ≤ 300 66 I

Lead ≤ 8.5 - 0.75 U

Magnesium (mg/L) - - 876

Nickel ≤ 8.3 - 9.8 U

Selenium ≤ 71 - 5.3 I

Silver ≤ 2.3 - 0.25 U

Zinc ≤ 86 - 6 U

Ortho-phosphate - - 0.31

Total Phosphorus - - 0.38

Ammonia - - 0.038

Unionized Ammonia - - < 0.02

Nitrate+Nitrite - - 0.013

Total Kjeldahl Nitrogen - - 0.87

Organic Nitrogen - - 0.83 c

Total Nitrogen - - 0.88 c

Dissolved Oxygen (mg/L) ≥ 4 s - 5.4

pH (S.U.) 6.5-8.5 s - 7.8

Conductivity (umhos/cm) - - 38,407

Temperature (C) - Report 38.5

BOD (mg/L) - - 1.8 AI

TSS (mg/L) - - 4 U

Turbidity (NTU) 29 t - 2

Flow (MGD) - Report 560 a

Value exceeds the Class III Marine Water Quality Criteria (62-302, F.A.C.) and/or Effluent Limits

a - Annual average

c - calculated value

s - Single sample

t - Shall not exceed 29 NTUs above background

A - Value reported is the mean of two or more determinations

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit.

U - Material analyzed for but not detected; value reported is the minimum detection limit

General Physical and Chemical Parameters

Table 2. Effluent limits, Class III Marine Criteria and chemical data for samples

from Tampa Bay Electric Company Bayside facility on June 27, 2005.

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)

5
Appendix 1. Bench Sheets 

6
Appendix 1. Bench Sheets (continued) 

7
Appendix 1. Bench Sheets (continued)

8
Appendix 1. Bench Sheets (continued) 

9
Appendix 1. Bench Sheets (continued)

10
Appendix 2. Chemical Analyses performed on the effluent from the Tampa Bay Electric Compan

Outfall D-001, sampled on June 27, 2005.

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:30 Outfall D-001 Bio-AGP/LimNut Algal Growth Potential 1.07 mg DryWt/L 6/27/2005 9:30 Outfall D-001 Bio-BOD Biochemical Oxygen Demand-5 Day,N-Inhib 1.8 mg/L 6/27/2005 9:30 Outfall D-001 Bio-Chl-a Chlorophyll-A, Monochromatic, Water 8.3 ug/L 6/27/2005 9:30 Outfall D-001 Bio-Chl-a Phaeophytin-A, Monochromatic, Water 0 ug/L 6/27/2005 9:30 Outfall D-001 Bio-Toxicology Bioassay-Acute-Screen-SW-Fish, LC50 100 LC50 6/27/2005 9:30 Outfall D-001 Bio-Toxicology Bioassay-Acute-Screen-SW-Mysid, LC50 100 LC50 6/27/2005 9:30 Outfall D-001 BNA-Water 1,2,4-Trichlorobenzene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 1,2-Dichlorobenzene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 1,3-Dichlorobenzene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 1,4-Dichlorobenzene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2,4,6-Trichlorophenol 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2,4-Dichlorophenol 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2,4-Dimethylphenol 54 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2,4-Dinitrophenol 16 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2,4-Dinitrotoluene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2,6-Dinitrotoluene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2-Chloronaphthalene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2-Chlorophenol 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2-Methyl-4,6-dinitrophenol 3.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 2-Nitrophenol 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 3,3'-Dichlorobenzidine 43 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4,4'-DDD 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4,4'-DDE 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4,4'-DDT 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4-Bromophenyl phenyl ether 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4-Chloro-3-methylphenol 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4-Chlorophenyl phenyl ether 2.2 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water 4-Nitrophenol 16 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Acenaphthene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Acenaphthylene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Aldrin 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water alpha-BHC 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Anthracene 1.1 ug/L 

11
Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:30 Outfall D-001 BNA-Water Benzidine 110 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Benzo(a)anthracene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Benzo(a)pyrene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Benzo(b)fluoranthene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Benzo(g,h,i)perylene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Benzo(k)fluoranthene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water beta-BHC 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Bis(2-chloroethoxy)methane 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Bis(2-chloroethyl)ether 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Bis(2-chloroisopropyl)ether 3.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Bis(2-ethylhexyl)phthalate 16 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Butyl benzyl phthalate 5.4 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Chrysene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water delta-BHC 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Dibenzo(a,h)anthracene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Dieldrin 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Diethyl phthalate 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Dimethyl phthalate 54 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Di-n-butyl phthalate 5.4 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Di-n-octyl phthalate 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Endosulfan I 4.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Endosulfan II 4.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Endosulfan sulfate 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Endrin 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Endrin aldehyde 4.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Fluoranthene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Fluorene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water gamma-BHC 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Heptachlor 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Heptachlor epoxide 1.6 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Hexachlorobenzene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Hexachlorobutadiene 3.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Hexachlorocyclopentadiene 3.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Hexachloroethane 3.3 ug/L 

12
Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:30 Outfall D-001 BNA-Water Indeno(1,2,3-cd)pyrene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Isophorone 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Naphthalene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Nitrobenzene 2.2 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water N-Nitrosodimethylamine 2.2 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water N-Nitrosodi-n-propylamine 2.2 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water N-Nitrosodiphenylamine 3.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Pentachlorophenol 3.3 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Phenanthrene 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Phenol 1.1 ug/L 6/27/2005 9:30 Outfall D-001 BNA-Water Pyrene 1.1 ug/L 6/27/2005 9:50 blank BNA-Water 1,2,4-Trichlorobenzene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 1,2-Dichlorobenzene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 1,3-Dichlorobenzene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 1,4-Dichlorobenzene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2,4,6-Trichlorophenol 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2,4-Dichlorophenol 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2,4-Dimethylphenol 50 ug/L 6/27/2005 9:50 blank BNA-Water 2,4-Dinitrophenol 15 ug/L 6/27/2005 9:50 blank BNA-Water 2,4-Dinitrotoluene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2,6-Dinitrotoluene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2-Chloronaphthalene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2-Chlorophenol 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 2-Methyl-4,6-dinitrophenol 3 ug/L 6/27/2005 9:50 blank BNA-Water 2-Nitrophenol 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 3,3'-Dichlorobenzidine 40 ug/L 6/27/2005 9:50 blank BNA-Water 4,4'-DDD 1.5 ug/L 6/27/2005 9:50 blank BNA-Water 4,4'-DDE 1.5 ug/L 6/27/2005 9:50 blank BNA-Water 4,4'-DDT 1.5 ug/L 6/27/2005 9:50 blank BNA-Water 4-Bromophenyl phenyl ether 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 4-Chloro-3-methylphenol 0.99 ug/L 6/27/2005 9:50 blank BNA-Water 4-Chlorophenyl phenyl ether 2 ug/L 6/27/2005 9:50 blank BNA-Water 4-Nitrophenol 15 ug/L 6/27/2005 9:50 blank BNA-Water Acenaphthene 0.99 ug/L 

13
Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:50 blank BNA-Water Acenaphthylene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Aldrin 1.5 ug/L 6/27/2005 9:50 blank BNA-Water alpha-BHC 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Anthracene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Benzidine 99 ug/L 6/27/2005 9:50 blank BNA-Water Benzo(a)anthracene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Benzo(a)pyrene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Benzo(b)fluoranthene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Benzo(g,h,i)perylene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Benzo(k)fluoranthene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water beta-BHC 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Bis(2-chloroethoxy)methane 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Bis(2-chloroethyl)ether 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Bis(2-chloroisopropyl)ether 3 ug/L 6/27/2005 9:50 blank BNA-Water Bis(2-ethylhexyl)phthalate 15 ug/L 6/27/2005 9:50 blank BNA-Water Butyl benzyl phthalate 5 ug/L 6/27/2005 9:50 blank BNA-Water Chrysene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water delta-BHC 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Dibenzo(a,h)anthracene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Dieldrin 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Diethyl phthalate 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Dimethyl phthalate 50 ug/L 6/27/2005 9:50 blank BNA-Water Di-n-butyl phthalate 5 ug/L 6/27/2005 9:50 blank BNA-Water Di-n-octyl phthalate 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Endosulfan I 4 ug/L 6/27/2005 9:50 blank BNA-Water Endosulfan II 4 ug/L 6/27/2005 9:50 blank BNA-Water Endosulfan sulfate 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Endrin 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Endrin aldehyde 4 ug/L 6/27/2005 9:50 blank BNA-Water Fluoranthene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Fluorene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water gamma-BHC 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Heptachlor 1.5 ug/L 6/27/2005 9:50 blank BNA-Water Heptachlor epoxide 1.5 ug/L 

14
Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:50 blank BNA-Water Hexachlorobenzene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Hexachlorobutadiene 3 ug/L 6/27/2005 9:50 blank BNA-Water Hexachlorocyclopentadiene 3 ug/L 6/27/2005 9:50 blank BNA-Water Hexachloroethane 3 ug/L 6/27/2005 9:50 blank BNA-Water Indeno(1,2,3-cd)pyrene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Isophorone 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Naphthalene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Nitrobenzene 2 ug/L 6/27/2005 9:50 blank BNA-Water N-Nitrosodimethylamine 2 ug/L 6/27/2005 9:50 blank BNA-Water N-Nitrosodi-n-propylamine 2 ug/L 6/27/2005 9:50 blank BNA-Water N-Nitrosodiphenylamine 3 ug/L 6/27/2005 9:50 blank BNA-Water Pentachlorophenol 3 ug/L 6/27/2005 9:50 blank BNA-Water Phenanthrene 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Phenol 0.99 ug/L 6/27/2005 9:50 blank BNA-Water Pyrene 0.99 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Alachlor 0.6 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Ametryn 0.05 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Atrazine 0.05 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Azinphos Methyl 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Bromacil 0.2 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Butylate 0.2 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Chlorpyrifos Ethyl 0.05 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Chlorpyrifos Methyl 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Diazinon 0.05 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Ethion 0.05 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Ethoprop 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Fenamiphos 0.2 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Fonofos 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Hexazinone 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Malathion 0.15 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Metalaxyl 0.25 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Metolachlor 0.5 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Metribuzin 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Mevinphos 0.2 ug/L 

15
Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:30 Outfall D-001 GC-Water Naled 0.8 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Norflurazon 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Parathion Ethyl 0.15 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Parathion Methyl 0.1 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Phorate 0.05 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Prometryn 0.15 ug/L 6/27/2005 9:30 Outfall D-001 GC-Water Simazine 0.05 ug/L 6/27/2005 9:50 blank GC-Water Alachlor 0.65 ug/L 6/27/2005 9:50 blank GC-Water Ametryn 0.054 ug/L 6/27/2005 9:50 blank GC-Water Atrazine 0.054 ug/L 6/27/2005 9:50 blank GC-Water Azinphos Methyl 0.11 ug/L 6/27/2005 9:50 blank GC-Water Bromacil 0.22 ug/L 6/27/2005 9:50 blank GC-Water Butylate 0.22 ug/L 6/27/2005 9:50 blank GC-Water Chlorpyrifos Ethyl 0.054 ug/L 6/27/2005 9:50 blank GC-Water Chlorpyrifos Methyl 0.11 ug/L 6/27/2005 9:50 blank GC-Water Diazinon 0.054 ug/L 6/27/2005 9:50 blank GC-Water Ethion 0.054 ug/L 6/27/2005 9:50 blank GC-Water Ethoprop 0.11 ug/L 6/27/2005 9:50 blank GC-Water Fenamiphos 0.22 ug/L 6/27/2005 9:50 blank GC-Water Fonofos 0.11 ug/L 6/27/2005 9:50 blank GC-Water Hexazinone 0.11 ug/L 6/27/2005 9:50 blank GC-Water Malathion 0.16 ug/L 6/27/2005 9:50 blank GC-Water Metalaxyl 0.27 ug/L 6/27/2005 9:50 blank GC-Water Metolachlor 0.54 ug/L 6/27/2005 9:50 blank GC-Water Metribuzin 0.11 ug/L 6/27/2005 9:50 blank GC-Water Mevinphos 0.22 ug/L 6/27/2005 9:50 blank GC-Water Naled 0.87 ug/L 6/27/2005 9:50 blank GC-Water Norflurazon 0.11 ug/L 6/27/2005 9:50 blank GC-Water Parathion Ethyl 0.16 ug/L 6/27/2005 9:50 blank GC-Water Parathion Methyl 0.11 ug/L 6/27/2005 9:50 blank GC-Water Phorate 0.054 ug/L 6/27/2005 9:50 blank GC-Water Prometryn 0.16 ug/L 6/27/2005 9:50 blank GC-Water Simazine 0.054 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Aluminum 89 ug/L 

16
Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:30 Outfall D-001 Metals-Water Arsenic 8 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water

Nutrients-Liquid 

Cadmium 0.5 ug/L 

6/27/2005 9:30 Outfall D-001 Metals-Water Calcium 271 mg/L 6/27/2005 9:30 Outfall D-001 Metals-Water Chromium 9.4 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Copper 5 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Iron 66 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Lead 0.75 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Magnesium 876 mg/L 6/27/2005 9:30 Outfall D-001 Metals-Water Nickel 9.8 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Selenium 5.3 ug/L 6/27/2005 9:30 Outfall D-001 Metals-Water Silver 0.25 ug/L 

6/27/2005 9:30 Outfall D-001 Metals-Water Zinc 6 ug/L 6/27/2005 9:50 blank Metals-Water Aluminum 5 ug/L 6/27/2005 9:50 blank Metals-Water Arsenic 4 ug/L 6/27/2005 9:50 blank Metals-Water Cadmium 0.05 ug/L 6/27/2005 9:50 blank Metals-Water Calcium 0.05 mg/L 6/27/2005 9:50 blank Metals-Water Chromium 2 ug/L 6/27/2005 9:50 blank Metals-Water Copper 1.1 ug/L 6/27/2005 9:50 blank Metals-Water Iron 10 ug/L 6/27/2005 9:50 blank Metals-Water Lead 0.3 ug/L 6/27/2005 9:50 blank Metals-Water Magnesium 0.01 mg/L 6/27/2005 9:50 blank Metals-Water Nickel 2 ug/L 6/27/2005 9:50 blank Metals-Water Selenium 0.5 ug/L 6/27/2005 9:50 blank Metals-Water Silver 0.025 ug/L 6/27/2005 9:50 blank Metals-Water Zinc 5.5 ug/L 6/27/2005 9:30 Outfall D-001 Nutrients-Liquid Ammonia-N 0.038 mg N/L 6/27/2005 9:30 Outfall D-001 Nutrients-Liquid Kjeldahl Nitrogen 0.87 mg N/L 6/27/2005 9:30 Outfall D-001 NO2NO3-N 0.013 mg N/L 6/27/2005 9:30 Outfall D-001 Nutrients-Liquid O-Phosphate-P 0.31 mg P/L 6/27/2005 9:30 Outfall D-001 Nutrients-Liquid Total-P 0.38 mg P/L 6/27/2005 9:30 Outfall D-001 Nutrients-Liquid TSS 4 mg/L 6/27/2005 9:30 Outfall D-001 Nutrients-Liquid Turbidity 2 NTU 6/27/2005 9:50 blank Nutrients-Liquid Ammonia-N 0.01 mg N/L 6/27/2005 9:50 blank Nutrients-Liquid Kjeldahl Nitrogen 0.06 mg N/L 

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Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 6/27/2005 9:50 blank Nutrients-Liquid NO2NO3-N 0.004 mg N/L 6/27/2005 9:50 blank Nutrients-Liquid O-Phosphate-P 0.004 mg P/L 6/27/2005 9:50 blank Nutrients-Liquid Total-P 0.02 mg P/L 

18
Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 000 809 11 12 0 506 2 7 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 000 809 11 12 0 506 2 7 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 000 809 11 12 0 506 2 7 18 S 19 S 20 2

66

Biological Analyses of Tampa Bay Electric Company Bayside

facility effluent sampled on June 27, 2005. NPDES #FL0000809. Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of Tampa Bay Electric Company Bayside facility

effluent sampled on June 27, 2005. NPDES #FL0000809. Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of Tampa Bay Electric Company

Bayside facility effluent sampled on June 27, 2005. NPDES

#FL0000809.

19