Acequinocyl Dietary Exposure and Risk Assessment

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UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

 

MEMORANDUM

Date: 30-NOV-2011 

SUBJECT: Acequinocyl. Chronic Aggregate Dietary (Food 

Risk Assessment for the Section 3 Registration Action

Vegetable; Succulent Shelled Beans; Cowpea Forage; Caneberry Subgroup 13

07A; Melon Subgroup 9A; Cucumber

07G; and Small Fruit Vine Climbing, Except Fuzzy Kiwifruit, Subgroup 13

PC Code: 006329

Decision No.: 448205

Petition No.: 1E7864

Risk Assessment Type: Dietary

TXR No.: NA

MRID No.: NA

FROM: Sarah J. Levy, Chemist

Risk Assessment Branch 1 (RAB1)/Health Effects Division (HED; 7509P)

THROUGH: Doug Dotson, Ph.D., 

Amelia Acierto, Chemist

Dietary Exposure Science Advisory Council (DESAC)

and

George F. Kramer, Ph.D.

RAB1/HED (7509P)

TO: Sarah J. Levy, Chemist/Risk Assessor

RAB1/HED (7509P)

and

Barbara Madden, RM 05

Registration Division (

Dietary Exposure and Risk Assessment

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Page 1 of 13

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, D.C. 20460 

OFFICE OF CHEMICAL SAFETY AND

 POLLUTION PREVENTION

Chronic Aggregate Dietary (Food + Drinking Water) Exposure and 

Risk Assessment for the Section 3 Registration Action Use on Succulent Soybean 

Vegetable; Succulent Shelled Beans; Cowpea Forage; Caneberry Subgroup 13

07A; Melon Subgroup 9A; Cucumber; Cherry; Low-Growing Berry Subgroup 13

G; and Small Fruit Vine Climbing, Except Fuzzy Kiwifruit, Subgroup 13

DP Barcode: D389990

Registration No.: 66330-38

Regulatory Action: Section 3

Dietary Case No.: 7621

CAS No.: 57960-19-7

40 CFR: §180.599

Sarah J. Levy, Chemist

Risk Assessment Branch 1 (RAB1)/Health Effects Division (HED; 7509P)

Ph.D., Chemist

Chemist

Exposure Science Advisory Council (DESAC)/HED (7509P)

George F. Kramer, Ph.D., Branch Senior Scientist

(7509P)

Sarah J. Levy, Chemist/Risk Assessor

(7509P)

Barbara Madden, RM 05

Division (RD; 7505P)

DP# 389990

_____________________________________________________________________________________________

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF CHEMICAL SAFETY AND

POLLUTION PREVENTION

Drinking Water) Exposure and 

Succulent Soybean 

Vegetable; Succulent Shelled Beans; Cowpea Forage; Caneberry Subgroup 13-

Growing Berry Subgroup 13-

G; and Small Fruit Vine Climbing, Except Fuzzy Kiwifruit, Subgroup 13-07F.

Section 3

Risk Assessment Branch 1 (RAB1)/Health Effects Division (HED; 7509P)

(7509P)
Acequinocyl Dietary Exposure and Risk Assessment DP# 389990

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Executive Summary

A chronic aggregate dietary (food + drinking water) exposure and risk assessment was conducted 

using the Dietary Exposure Evaluation Model (DEEM-FCIDTM, Version 2.03) which uses food 

consumption data from the U.S. Department of Agriculture’s Continuing Surveys of Food 

Intakes by Individuals (CSFII) from 1994-1996 and 1998. The analysis was performed to 

support a requested Section 3 registration use of the miticide acequinocyl (2-(acetyloxy)-3-

dodecyl-1,4-naphthalenedione) on the following crops: succulent soybean vegetable; succulent 

shelled beans; cowpea forage; caneberry subgroup 13-07A; melon subgroup 9A; cucumber; 

cherry; low-growing berry subgroup 13-07G; and small fruit vine climbing, except fuzzy

kiwifruit, subgroup 13-07F. An acute dietary assessment was not conducted for acequinocyl 

because an endpoint of concern attributable to a single dose was not identified; therefore, an 

aRfD was not established. A cancer dietary assessment was not conducted because acequinocyl 

was classified as not likely to be carcinogenic to humans.

 

Chronic Dietary (Food + Drinking Water) Exposure Results and Characterization

Tolerance-level residues and 100 percent crop treated (%CT) data were used in the chronic 

dietary assessment. DEEMver. 7.81 default processing factors were used with the exception of 

those for grape juice and raisins (see “Residue Information” Section of this document for 

details). For the chronic dietary assessment, the general U.S. population and all population 

subgroups have risk estimates that are not of concern to HED. The chronic dietary risk estimate 

for the U.S. population is 13% of the chronic population-adjusted dose (cPAD). The highest 

exposed population subgroup (children 1-2 years old) occupies 55% of the cPAD. The use of 

anticipated residues (ARs), empirical processing factors, and %CT would refine further HED’s 

exposure and risk estimates; however, refinement is not needed at this time.

I. Introduction

Dietary risk assessment incorporates both exposure and toxicity of a given pesticide. For acute 

and chronic assessments, the risk is expressed as a percentage of a maximum acceptable dose 

(i.e., the dose which HED has concluded will result in no unreasonable adverse health effects). 

This dose is referred to as the population-adjusted dose (PAD). The PAD is equivalent to point 

of departure (POD, NOAEL, LOAEL, e.g.) divided by the required uncertainty or safety factors.

For acute and non-cancer chronic exposures, HED is concerned when estimated dietary risk 

exceeds 100% of the PAD. HED is generally concerned when estimated cancer risk exceeds one 

in one million. References which discuss the acute and chronic risk assessments in more detail 

are available on the EPA/pesticides web site: “Available Information on Assessing Exposure 

from Pesticides, A User’s Guide,” 21-JUN-2000, web link: http://www.epa.gov/fedrgstr/EPAPEST/2000/July/Day-12/6061.pdf; or see SOP 99.6 (20-AUG-1999).

The most recent dietary risk assessment for acequinocyl was conducted by Sarah Levy (25-

AUG-2010; D369101).
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II. Residue Information

Under PP1E7864, the Interregional Research Project No.4 (IR-4), on behalf of the Agricultural 

Experiment Station of TX, CA, OR, PA, GA, NM, OK, TN, UT, MI, FL, and AZ, is requesting 

the establishment of tolerances for combined residues of the insecticide acequinocyl, and its 

metabolite, 2-dodecyl-3-hydroxy-1,4-naphthoquinone (acequinocyl-OH), expressed as 

acequinocyl equivalents in/on the following commodities: succulent soybean vegetable; 

succulent shelled beans; cowpea forage; caneberry subgroup 13-07A; melon subgroup 9A; 

cucumber; cherry; low-growing berry subgroup 13-07G; and small fruit vine climbing, except 

fuzzy kiwifruit, subgroup 13-07F. No new data were submitted to support tolerances on 

succulent soybean vegetables, low growing berry 13-07G crop subgroup, or small fruit vine 

climbing subgroup 13-07F, except fuzzy kiwifruit. The petitioner has requested that existing 

tolerances be expanded to these respective crops or crop groups.

Permanent tolerances are currently established under 40 CFR §180.599(a) for the combined 

residues of acequinocyl and its metabolite, acequinocyl-OH, expressed as acequinocyl 

equivalents, in/on a number of plant and livestock commodities. Tolerances for plant 

commodities range from 0.02 ppm (tree nuts and pistachios) to 30 ppm (citrus oil), and 

tolerances for livestock commodities are established at 0.02 ppm in the fat and liver of cattle, 

goat, horse, and sheep.

Based on the structural similarity of acequinocyl-OH to parent and the presence of acequinocylOH at quantifiable levels in crops, the HED Metabolism Assessment Review Committee 

(MARC) concluded that parent and acequinocyl-OH are the residues of concern for risk 

assessment and tolerance expression in apples, oranges, and eggplant commodities (Memo, S. 

Levy, 07-JAN-2004; D297872). For purposes of this petition, HED will allow translation of

these results to caneberries, cantaloupe, cherry, and cucumber as these are fruit crops. In 

addition, HED is willing to translate the results to succulent shelled beans because beans are lowconsumption food items. However, if in the future the petitioner requests new uses where apple, 

orange, and eggplant metabolism data cannot be translated, HED may ask for additional plant 

metabolism data.

The qualitative nature of acequinocyl residues in ruminants is understood based on an adequate 

goat metabolism study (Memo, S. Levy, 10-MAR-2004; D284757). The metabolism of 

acequinocyl in goats appears to involve the loss of the acetyloxyl moiety to form acequinocylOH and partial cleavage of the dodecyl side chain to form AKM-15. Opening and degradation 

of the quinone ring was also evidenced by the presence of AKM-18 and phthalic acid. The 

MARC concluded that for tolerance expression, the residues of concern in livestock commodities 

include parent and acequinocyl-OH. For risk assessment purposes, the residues of concern in 

livestock commodities are parent and acequinocyl-OH (for liver and kidney, metabolite AKM-15 

is to be included as well (Memo, S. Levy, et al., 07-JAN-2004; D297872)). 

Metabolism data on poultry are not required at the present time as none of the proposed or 

existing uses of acequinocyl are on crops having regulated poultry feedstuffs.
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Table 1. Residues of Concern in Crops, Ruminants, and Drinking Water.

Matrix Tolerance Expression Residues for Risk Assessment

Fruit Crops Acequinocyl + Acequinocyl-OH (R1) Acequinocyl + Acequinocyl-OH (R1)

Ruminants Acequinocyl + Acequinocyl-OH (R1) Acequinocyl + Acequinocyl-OH (R1). In 

liver and kidney, also include AKM-15.

Rotational Crops Not Determined* Not Determined*

Drinking Water NA** Acequinocyl + Acequinocyl-OH (R1)

* A confined rotational crop study has been reviewed and a 30-day plant-back interval has been recommended 

(Memo, S. Levy, 25-JUN-2008; DP# 351336).

** NA = not applicable.

Residue Data used for the Chronic Assessment:

Tolerance-level residues and 100%CT data were used in the chronic dietary assessment. 

DEEMver. 7.81 default processing factors were used with the exception of those for grape 

juice and raisins. As residues were reduced in both grape juice (<0.02x) and raisin (0.15x), 

separate tolerances are not required for these commodities. The DEEMver. 7.81 default 

processing factors for juice and raisin were set to 1 as concentration was shown not to occur 

(Memo, S. Levy, 05-SEP-2007; D342805).

As cowpea forage and hay are significant feedstuff for livestock (dairy cattle only), the 

maximum reasonable balanced diet (MRBD) for acequinocyl was recalculated for this petition 

using the Agency’s most recent guidance on constructing reasonably balanced livestock diets 

(Science Advisory Council for Chemistry (ChemSAC) memo, 30-JUN-2008). The beef cattle 

MRBDs did not need to be recalculated as cowpea forage and hay are not beef feed items. The 

feeding study data indicate that the currently established tolerance level of 0.02 ppm for residues 

in cattle, goat, horse, and sheep fat is appropriate. Furthermore, the 0.02-ppm residue level is 

sufficient to cover residues of the additional metabolite (AKM-15) of concern in ruminant liver 

and kidney. The established 0.02-ppm residue level in cattle, goat, horse, and sheep liver are 

appropriate; however, the commodity definition should be changed to “meat byproducts” rather

than “liver.” With the establishment of the cattle, goat, horse, and sheep tolerance in meat 

byproducts, RD should remove the tolerance for liver. See Table 2 for a summary of the data 

used in the chronic assessment.

Table 2. Data and Residue Estimates Used in the Acequinocyl Chronic Dietary Analyses.

Commodity Data Source

(MRID) %CT ProcessingFactors

HED-Recommended 

Tolerance (ppm)

Bean, succulent shelled148462901

100

NA 0.30

Caneberry subgroup 13-07A 48462902 NA 4.0

Melon subgroup 9A 48462903 NA 0.15

Cucumber 48462905 NA 0.15

Cherry, sweet 48462904 NA 0.50

Cherry, tart2 NA 1.0

Soybean, vegetable, succulent Translated from 

established ediblepodded bean tolerance

NA 0.25
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Table 2. Data and Residue Estimates Used in the Acequinocyl Chronic Dietary Analyses.

Commodity Data Source

(MRID) %CT ProcessingFactors

HED-Recommended 

Tolerance (ppm)

Low growing berry subgroup13-

07G3

Translated from 

established strawberry

tolerance

NA 0.50

Small fruit vine climbing 

subgroup 13-07F, except fuzzy 

kiwifruit

Translated from 

established grape

tolerance

NA 1.6

Meat byproducts of cattle, goat, 

horse, and sheep

Extended from liver NA 0.02

1

 HED is recommending for cowpea forage and cowpea hay tolerances of 6.0 ppm and 18 ppm, respectively, 

however, these are feed items and are not in DEEM-FCIDTM.

2

 The value for cherry, tart was used as the input in DEEM-FCIDTM as the HED-recommended tolerance value was 

higher than the value for cherry, sweet.

3

 The established strawberry tolerance is 0.40 ppm; however, for purposes of harmonization with Canada, HED is 

recommending to increase the tolerance to 0.50 ppm for this crop subgroup.

III. Drinking Water Data

The drinking water residues used in the dietary risk assessment were provided by the 

Environmental Fate and Effects Division (EFED) (M. Lowit, 07-SEP-2011; D389520) and 

incorporated directly into this dietary assessment. Water residues were incorporated directly into 

DEEM-FCIDTM under the food categories “water, direct, all sources” and “water, indirect, all 

sources.”

Acequinocyl can reach surface water via spray drift and less likely via runoff. Due to its high Kd

values it is expected to exhibit low mobility in soil and low potential to leach into groundwater. 

Once acequinocyl has entered surface water, it is not likely to persist as it will hydrolyze to form 

hydroxy-acequinocyl (also known as R-1).

There are no drinking water monitoring data available for acequinocyl or its metabolite 

acequinocyl-OH (R1), the residues of concern. Groundwater estimated drinking water 

concentrations (EDWCs) were generated for acequinocyl using the Screening Concentration in 

Ground Water (SCI-GROW) Model. Surface water EDWCs were generated using the Pesticide 

Root Zone Model/Exposure Analysis Modeling System (PRZM/EXAMS) model for all proposed 

uses except cranberry (cranberries are part of the low growing berries subgroup, 13-07G). 

Cranberry harvesting may involve field flooding and PRZM/EXAMS is not currently 

parameterized to assess this type of scenario; therefore, cranberry was assessed using the 

provisional cranberry model (note that since the provisional cranberry model only uses a one 

year simulation, the highest 30-year annual average concentration is not available for the 

cranberry scenario).

Similar to previous drinking water assessments provided by EFED, two approaches were used in 

performing the drinking water assessment for proposed acequinocyl uses. In Approach #1, 

acequinocyl was conservatively assumed to be stable to all routes of degradation to account for 

the uncertainties associated with the environmental fate data. In Approach #2, total (acequinocyl 
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+ acequinocyl-OH) residues were modeled with the assumption that the half-lives of acequinocyl 

and R1 are valid and the total toxic residues were modeled to address toxicity concerns with R1.

The EDWCs are summarized below in Table 3. Surface water EDWCs for the proposed uses are 

higher than those previously reported for both approaches. The ground water EDWC for the 

current assessment is the same as the last assessment.

Table 3. Recommended EDWCs of Acequinocyl Derived from the Previous Uses.

Drinking water 

source (model)

Proposed 

Use/Scenario3

EDWCs from Surface Water Sources (ug/L) 1,2

Approach 1

(assume no acequinocyl degradation)

Approach 2

(combined acequinocyl and R1)

1-in-10 

year acute

1-in-10 year 

chronic

30-year 

mean

1-in-10 

year acute

1-in-10 year 

chronic

30-year 

mean

Surface water

(PRZM/EXAMS) Cherry/MI cherry 6.69

(25.89)4

6.69 

(17.84)4

6.69

(10.19)4 5.59 0.42 0.26

Surface water

(PRZM/EXAMS)

Small fruit (vine 

climbing, except fuzzy 

kiwifruit, 13-07F)/NY 

grape

6.69

(23.12)4

6.69 

(15.34)4

6.69 

(9.57)4 4.22 0.38 0.27

Surface water

(Provisional 

Cranberry Model)

Cranberry/Provisional 

Cranberry Model 4.24 3.75 -- 3.815

1.365

--

Groundwater 

(SCIGROW) All proposed uses 3.6 x 10-3

<3.6 x 10-3<3.6 x 10-33.6 x 10-3<3.6 x 10-3<3.6 x 10-3

1

 Bolded values are the recommended EDWCs by EFED.

2

 A default PCA of 0.87 was applied.

3

 Maximum of 2 applications of 0.3 lb ai/A for all proposed uses.

4

 EDWC (shown in parentheses) exceeds the solubility limit of acequinocyl (6.69 μg/L at 20°C); therefore, the 

recommended EDWC is 6.69 ppb. 

5

 Based on a one-year simulation; 1-10 year calculation is not applicable.

Since the surface water EDWCs are higher than the groundwater EDWC, HED selected the 

surface water value for use in the chronic dietary assessment. The EDWC derived via Approach 

#1 represents the upper-bound value (6.69 ppb) while that derived via Approach #2 represents 

the lower-bound value (1.36 ppb). The 6.69 ppb value was used in this dietary assessment as this 

value assures a conservative assessment.

IV. DEEM-FCIDTM Program and Consumption Information

An acequinocyl chronic dietary exposure assessment was conducted using DEEM-FCIDTM,

Version 2.03 which incorporates consumption data from USDA’s CSFII, 1994-1996 and 1998. 

The 1994-96, 98 data are based on the reported consumption of more than 20,000 individuals 

over two non-consecutive survey days. Foods “as consumed” (e.g., apple pie) are linked to 

EPA-defined food commodities (e.g., apples, peeled fruit - cooked; fresh or N/S; baked; or wheat 

flour - cooked; fresh or N/S, baked) using publicly available recipe translation files developed 

jointly by USDA/ARS and EPA. For chronic exposure assessment, consumption data are 

averaged for the entire U.S. population and within population subgroups, but for acute exposure 

assessment are retained as individual consumption events. Based on analysis of the 1994-96, 98 

CSFII consumption data, which took into account dietary patterns and survey respondents, HED 

concluded that it is most appropriate to report risk for the following population subgroups: the 
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general U.S. population, all infants (<1 year old), children 1-2, children 3-5, children 6-12, youth 

13-19, adults 20-49, females 13-49, and adults 50+ years old.

For chronic dietary exposure assessment, an estimate of the residue level in each food or foodform (e.g., orange or orange juice) on the food commodity residue list is multiplied by the 

average daily consumption estimate for that food/food form to produce a residue intake estimate. 

The resulting residue intake estimate for each food/food form is summed with the residue intake 

estimates for all other food/food forms on the commodity residue list to arrive at the total 

average estimated exposure. Exposure is expressed in mg/kg body weight/day and as a percent 

of the cPAD. This procedure is performed for each population subgroup.

V. Toxicological Information

On 14-OCT-2003, the HED Hazard Identification Assessment Review Committee (HIARC) 

reviewed the recommendations of the toxicology reviewer for acequinocyl with regard to the 

acute and chronic RfDs and the toxicological endpoint selection for use as appropriate in 

occupational/residential exposure risk assessments. The potential for increased susceptibility of 

infants and children from exposure to acequinocyl was also evaluated as required by the Food 

Quality Protection Act (FQPA) of 1996 under the 2002 OPP 10X Guidance. Table 4 is a 

summary of the toxicology doses and endpoints selected that are relevant to dietary exposure 

assessment (13-NOV-2003; TXR# 0052234). The acequinocyl risk assessment team confirmed 

that the endpoints selected previously remain appropriate.

Table 4. Summary of Toxicological Doses and Endpoints for Acequinocyl for Use in Dietary Exposure Risk 

Assessment.1

Exposure/

Scenario

Point of 

Departure

Uncertainty/

FQPA Safety 

Factors

RfD, PAD, Level of 

Concern 

Study and Toxicological Effects

Acute Dietary 

(General 

Population, 

including 

Infants and 

Children)

N/A N/A N/A An endpoint of concern (effect) 

attributable to a single dose was 

not identified in the database. 

Quantification of acute risk to 

general population including 

infants and children is not 

required.

Chronic 

Dietary 

(All 

Populations)

NOAEL= 

2.7 

mg/kg/day

UFA= 10x

UFH=10x

FQPA SF= 1x

cPAD = cRfD = 

0.027 mg/kg/day 

18-month carcinogenicity study in 

mice;

LOAEL=7.0 mg/kg/day based on 

clinical chemistry and microscopic 

nonneoplastic lesions (brown 

pigmented cells and perivascular 

inflammatory cells in liver)

Cancer (oral, dermal, inhalation) Classification: “Not likely to be Carcinogenic to Humans.”

1

 UF = uncertainty factor, UFA = extrapolation from animal to human (interspecies), UFH = potential variation in 

sensitivity among members of the human population (intraspecies), FQPA SF = FQPA Safety Factor, NOAEL = noobserved adverse-effect level, LOAEL = lowest-observed adverse-effect level, RfD = reference dose (a = acute, c = 

chronic), PAD = population-adjusted dose, N/A = Not Applicable. 
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VI. Results/Discussion 

As stated above, for acute and chronic assessments, HED is concerned when dietary risk exceeds 

100% of the PAD. The DEEM-FCIDTM analyses estimate the dietary exposure of the U.S. 

population and various population subgroups. The results reported in Table 5 are for the general 

U.S. Population, all infants (<1 year old), children 1-2, children 3-5, children 6-12, youth 13-19, 

females 13-49, adults 20-49, and adults 50+ years. An acute dietary assessment was not 

conducted for acequinocyl because an endpoint of concern attributable to a single dose was not 

identified; therefore, an aRfD was not established. A cancer dietary assessment was not 

conducted because acequinocyl was classified as not likely to be carcinogenic to humans.

The chronic dietary exposure assessment (using tolerance level residues and 100% CT 

information for all registered and proposed uses) was conducted for the general U.S. population 

and various population subgroups. Drinking water was incorporated directly into the dietary

assessment using the chronic concentration for ground water generated by the PRZM/EXAMS 

model. This assessment concludes that the chronic dietary risk estimates are not of concern to 

HED for the general U.S. population (13% cPAD) and all population subgroups. The most 

highly exposed population subgroup is children 1-2 years old at 55% of the cPAD.

Table 5. Summary of Dietary (Food + Drinking Water) Exposure and Risk for Acequinocyl.

Population Subgroup

Chronic Dietary1

Dietary Exposure

(mg/kg/day) % cPAD

U.S. Population (total) 0.003627 13

All Infants (<1 year old) 0.006513 24

Children 1-2 years old 0.014761 55

Children 3-5 years old 0.010370 38

Children 6-12 years old 0.005172 19

Youth 13-19 years old 0.002825 11

Adults 20-49 years old 0.002525 9.4

Adults 50+ years old 0.002473 9.2

Females 13-49 years old 0.002563 9.5

1 Chronic dietary endpoint of 0.027 mg/kg/day applies to the general U.S. population and all population subgroups. The values 

for the highest exposed population are bolded.

VII. Characterization of Inputs/Outputs

The chronic analysis assumed tolerance-level residues and 100%CT data. DEEMver. 7.81 

default processing factors were used with the exception of those for grape juice and raisins. This 

analysis is considered very conservative and could be refined through the use of ARs for all 

commodities, %CT data for registered commodities, and/or additional empirical processing 

factors.
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VIII. Conclusions

A chronic dietary exposure and risk assessment was conducted for the proposed food uses and 

permanent tolerances of acequinocyl, including potential exposure from drinking water. 

The chronic dietary (food + drinking water) exposure to acequinocyl is below HED’s level of 

concern for the general U.S. population and all population subgroups. The chronic dietary risk

utilized 13% cPAD for the general U.S. population and 55% of the cPAD for children 1-2 years 

old, the most highly exposed population subgroup. An acute dietary assessment was not 

conducted for acequinocyl because an endpoint of concern attributable to a single dose was not 

identified; therefore, an aRfD was not established. A cancer dietary assessment was not 

conducted because acequinocyl was classified as not likely to be carcinogenic to humans.

IX. List of Attachments

Attachment 1: Chronic Food + Drinking Water Residue Input File.

Attachment 2: Chronic Results File.

cc: M. Lowit (EFED/ERB1; 7507P)

RDI: DESAC (09-NOV-2011); G.F. Kramer (09-NOV-2011)

S. Levy:S10953:PY1:(703)305-0783:7590P
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Attachment 1: Chronic Food + Drinking Water Residue Input File.

Filename: C:\Documents and Settings\slevy\Desktop\006329c_SL.R98

Chemical: Acequinocyl

RfD(Chronic): .027 mg/kg bw/day NOEL(Chronic): 2.7 mg/kg bw/day

RfD(Acute): 0 mg/kg bw/day NOEL(Acute): 0 mg/kg bw/day

Date created/last modified: 11-16-2011/08:07:44/8 Program ver. 2.03

Comment: No acute dietary endpoint; not carcinogen. cRfD=cPAD

--------------------------------------------------------------------------------

 EPA Crop Def Res Adj.Factors Comment

 Code Grp Commodity Name (ppm) #1 #2 

-------- ---- ------------------------------- ---------- ------ ------ -------

06003470 6 Soybean, seed 0.250000 1.000 1.000 1E7864

06010430 6A Bean, snap, succulent 0.250000 1.000 1.000 9E7598

06010431 6A Bean, snap, succulent-babyfood 0.250000 1.000 1.000 9E7598

06020310 6B Bean, broad, succulent 0.250000 1.000 1.000 1E7864

06020330 6B Bean, cowpea, succulent 0.250000 1.000 1.000 1E7864

06020370 6B Bean, lima, succulent 0.250000 1.000 1.000 1E7864

08001480 8 Eggplant 0.700000 1.000 1.000 9E7598

08002340 8 Okra 0.700000 1.000 1.000 9E7598

08002700 8 Pepper, bell 0.700000 1.000 1.000 9E7598

08002701 8 Pepper, bell-babyfood 0.700000 1.000 1.000 9E7598

08002710 8 Pepper, bell, dried 0.700000 1.000 1.000 9E7598

08002711 8 Pepper, bell, dried-babyfood 0.700000 1.000 1.000 9E7598

08002720 8 Pepper, nonbell 0.700000 1.000 1.000 9E7598

08002721 8 Pepper, nonbell-babyfood 0.700000 1.000 1.000 9E7598

08002730 8 Pepper, nonbell, dried 0.700000 1.000 1.000 9E7598

08003740 8 Tomatillo 0.700000 1.000 1.000 9E7598

08003750 8 Tomato 0.700000 1.000 1.000 9E7598

08003751 8 Tomato-babyfood 0.700000 1.000 1.000 9E7598

08003760 8 Tomato, paste 0.700000 5.400 1.000 9E7598

08003761 8 Tomato, paste-babyfood 0.700000 5.400 1.000 9E7598

08003770 8 Tomato, puree 0.700000 3.300 1.000 9E7598

08003771 8 Tomato, puree-babyfood 0.700000 3.300 1.000 9E7598

08003780 8 Tomato, dried 0.700000 14.300 1.000 9E7598

08003781 8 Tomato, dried-babyfood 0.700000 14.300 1.000 9E7598

08003790 8 Tomato, juice 0.700000 1.500 1.000 9E7598

09010750 9A Cantaloupe 0.150000 1.000 1.000 1E7864

09010800 9A Casaba 0.150000 1.000 1.000 1E7864

09011870 9A Honeydew melon 0.150000 1.000 1.000 1E7864

09013990 9A Watermelon 0.150000 1.000 1.000 1E7864

09014000 9A Watermelon, juice 0.150000 1.000 1.000 1E7864

09021350 9B Cucumber 0.150000 1.000 1.000 1E7864

10001060 10 Citrus citron 0.200000 1.000 1.000 

10001070 10 Citrus hybrids 0.200000 1.000 1.000 

10001080 10 Citrus, oil 30.000000 1.000 1.000 

10001800 10 Grapefruit 0.200000 1.000 1.000 

10001810 10 Grapefruit, juice 0.200000 2.100 1.000 

10001970 10 Kumquat 0.200000 1.000 1.000 

10001990 10 Lemon 0.200000 1.000 1.000 

10002000 10 Lemon, juice 0.200000 2.000 1.000 

10002001 10 Lemon, juice-babyfood 0.200000 2.000 1.000 

10002010 10 Lemon, peel 0.200000 1.000 1.000 

10002060 10 Lime 0.200000 1.000 1.000 

10002070 10 Lime, juice 0.200000 2.000 1.000 

10002071 10 Lime, juice-babyfood 0.200000 2.000 1.000 

10002400 10 Orange 0.200000 1.000 1.000 

10002410 10 Orange, juice 0.200000 1.800 1.000 

10002411 10 Orange, juice-babyfood 0.200000 1.800 1.000 

10002420 10 Orange, peel 0.200000 1.000 1.000 

10003070 10 Pummelo 0.200000 1.000 1.000 

10003690 10 Tangerine 0.200000 1.000 1.000 

10003700 10 Tangerine, juice 0.200000 2.300 1.000 

11000070 11 Apple, fruit with peel 0.400000 1.000 1.000 

11000080 11 Apple, peeled fruit 0.400000 1.000 1.000 

11000081 11 Apple, peeled fruit-babyfood 0.400000 1.000 1.000 
Acequinocyl Dietary Exposure and Risk Assessment DP# 389990

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Page 11 of 13

11000090 11 Apple, dried 0.400000 8.000 1.000 

11000091 11 Apple, dried-babyfood 0.400000 8.000 1.000 

11000100 11 Apple, juice 0.400000 1.300 1.000 

11000101 11 Apple, juice-babyfood 0.400000 1.300 1.000 

11000110 11 Apple, sauce 0.400000 1.000 1.000 

11000111 11 Apple, sauce-babyfood 0.400000 1.000 1.000 

11001290 11 Crabapple 0.400000 1.000 1.000 

11002100 11 Loquat 0.400000 1.000 1.000 

11002660 11 Pear 0.400000 1.000 1.000 

11002661 11 Pear-babyfood 0.400000 1.000 1.000 

11002670 11 Pear, dried 0.400000 6.250 1.000 

11002680 11 Pear, juice 0.400000 1.000 1.000 

11002681 11 Pear, juice-babyfood 0.400000 1.000 1.000 

11003100 11 Quince 0.400000 1.000 1.000 

12000900 12 Cherry 1.000000 1.000 1.000 1E7864

12000901 12 Cherry-babyfood 1.000000 1.000 1.000 1E7864

12000910 12 Cherry, juice 1.000000 1.500 1.000 1E7864

12000911 12 Cherry, juice-babyfood 1.000000 1.500 1.000 1E7864

13010550 13A Blackberry 4.000000 1.000 1.000 1E7864

13010560 13A Blackberry, juice 4.000000 1.000 1.000 1E7864

13010561 13A Blackberry, juice-babyfood 4.000000 1.000 1.000 1E7864

13010580 13A Boysenberry 4.000000 1.000 1.000 1E7864

13011420 13A Dewberry 4.000000 1.000 1.000 1E7864

13012080 13A Loganberry 4.000000 1.000 1.000 1E7864

13013200 13A Raspberry 4.000000 1.000 1.000 1E7864

13013201 13A Raspberry-babyfood 4.000000 1.000 1.000 1E7864

13013210 13A Raspberry, juice 4.000000 1.000 1.000 1E7864

13013211 13A Raspberry, juice-babyfood 4.000000 1.000 1.000 1E7864

13020570 13B Blueberry 0.500000 1.000 1.000 

13020571 13B Blueberry-babyfood 0.500000 1.000 1.000 

14000030 14 Almond 0.020000 1.000 1.000 

14000031 14 Almond-babyfood 0.020000 1.000 1.000 

14000040 14 Almond, oil 0.020000 1.000 1.000 

14000041 14 Almond, oil-babyfood 0.020000 1.000 1.000 

14000590 14 Brazil nut 0.020000 1.000 1.000 

14000680 14 Butternut 0.020000 1.000 1.000 

14000810 14 Cashew 0.020000 1.000 1.000 

14000920 14 Chestnut 0.020000 1.000 1.000 

14001550 14 Filbert 0.020000 1.000 1.000 

14001560 14 Filbert, oil 0.020000 1.000 1.000 

14001850 14 Hickory nut 0.020000 1.000 1.000 

14002130 14 Macadamia nut 0.020000 1.000 1.000 

14002690 14 Pecan 0.020000 1.000 1.000 

14002820 14 Pistachio 0.020000 1.000 1.000 

14003910 14 Walnut 0.020000 1.000 1.000 

21000460 M Beef, meat byproducts 0.020000 1.000 1.000 

21000461 M Beef, meat byproducts-babyfood 0.020000 1.000 1.000 

21000470 M Beef, fat 0.020000 1.000 1.000 

21000471 M Beef,fat-babyfood 0.020000 1.000 1.000 

21000480 M Beef, kidney 0.020000 1.000 1.000 

21000490 M Beef, liver 0.020000 1.000 1.000 

21000491 M Beef, liver-babyfood 0.020000 1.000 1.000 

23001710 M Goat, fat 0.020000 1.000 1.000 

23001720 M Goat, kidney 0.020000 1.000 1.000 

23001730 M Goat, liver 0.020000 1.000 1.000 

26003400 M Sheep, meat byproducts 0.020000 1.000 1.000 

26003410 M Sheep, fat 0.020000 1.000 1.000 

26003411 M Sheep, fat-babyfood 0.020000 1.000 1.000 

26003420 M Sheep, kidney 0.020000 1.000 1.000 

26003430 M Sheep, liver 0.020000 1.000 1.000 

86010000 O Water, direct, all sources 0.006690 1.000 1.000 1E7864

86020000 O Water, indirect, all sources 0.006690 1.000 1.000 1E7864

95001300 O Cranberry 0.500000 1.000 1.000 1E7864

95001301 O Cranberry-babyfood 0.500000 1.000 1.000 1E7864

95001310 O Cranberry, dried 0.500000 1.000 1.000 1E7864

95001320 O Cranberry, juice 0.500000 1.100 1.000 1E7864

95001321 O Cranberry, juice-babyfood 0.500000 1.100 1.000 1E7864
Acequinocyl Dietary Exposure and Risk Assessment DP# 389990

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Page 12 of 13

95001750 O Grape 1.600000 1.000 1.000 

95001760 O Grape, juice 1.600000 1.000 1.000 

95001761 O Grape, juice-babyfood 1.600000 1.000 1.000 

95001770 O Grape, leaves 1.600000 1.000 1.000 

95001780 O Grape, raisin 1.600000 1.000 1.000 

95001790 O Grape, wine and sherry 1.600000 1.000 1.000 

95001880 O Hop 4.000000 1.000 1.000 9E7598

95001950 O Kiwifruit 1.600000 1.000 1.000 1E7864

95003590 O Strawberry 0.500000 1.000 1.000 1E7864

95003591 O Strawberry-babyfood 0.500000 1.000 1.000 1E7864

95003600 O Strawberry, juice 0.500000 1.000 1.000 1E7864

95003601 O Strawberry, juice-babyfood 0.500000 1.000 1.000 1E7864
Acequinocyl Dietary Exposure and Risk Assessment DP# 389990

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Page 13 of 13

Attachment 2: Chronic Results File.

U.S. Environmental Protection Agency Ver. 2.00

DEEM-FCID Chronic analysis for ACEQUINOCYL (1994-98 data)

Residue file name: C:\Documents and Settings\slevy\Desktop\006329c_SL.R98

 Adjustment factor #2 used.

Analysis Date 11-16-2011/09:05:55 esidue file dated: 11-16-2011/08:07:44/8

Reference dose (RfD, Chronic) = .027 mg/kg bw/day

COMMENT 1: No acute dietary endpoint; not carcinogen. cRfD=cPAD

==========================================================================

 Total exposure by population subgroup

--------------------------------------------------------------------------

 Total Exposure

 -----------------------------------

 Population mg/kg Percent of 

 Subgroup body wt/day Rfd 

----------------------------------- ------------- ---------------

U.S. Population (total) 0.003627 13.4%

U.S. Population (spring season) 0.003565 13.2%

U.S. Population (summer season) 0.003721 13.8%

U.S. Population (autumn season) 0.003572 13.2%

U.S. Population (winter season) 0.003653 13.5%

Northeast region 0.004098 15.2%

Midwest region 0.003612 13.4%

Southern region 0.003232 12.0%

Western region 0.003850 14.3%

Hispanics 0.003984 14.8%

Non-hispanic whites 0.003597 13.3%

Non-hispanic blacks 0.003467 12.8%

Non-hisp/non-white/non-black 0.003724 13.8%

All infants (< 1 year) 0.006513 24.1%

Nursing infants 0.003204 11.9%

Non-nursing infants 0.007769 28.8%

Children 1-6 yrs 0.011306 41.9%

Children 7-12 yrs 0.004806 17.8%

Females 13-19 (not preg or nursing) 0.002860 10.6%

Females 20+ (not preg or nursing) 0.002497 9.2%

Females 13-50 yrs 0.002791 10.3%

Females 13+ (preg/not nursing) 0.002783 10.3%

Females 13+ (nursing) 0.002812 10.4%

Males 13-19 yrs 0.002783 10.3%

Males 20+ yrs 0.002507 9.3%

Seniors 55+ 0.002472 9.2%

Children 1-2 yrs 0.014761 54.7%

Children 3-5 yrs 0.010370 38.4%

Children 6-12 yrs 0.005172 19.2%

Youth 13-19 yrs 0.002825 10.5%

Adults 20-49 yrs 0.002525 9.4%

Adults 50+ yrs 0.002473 9.2%

Females 13-49 yrs 0.002563 9.5%