CERTIFICATIONS
        OF THE CHIEF EXECUTIVE OFFICER AND CHIEF FINANCIAL OFFICER
 UNDER
        SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002
  I,
        John
        S. Kovach, certify that:
  1.
        I have
        reviewed this Quarterly Report on Form 10-Q for the quarterly period ended
        September 30, 2008 of Lixte Biotechnology Holdings, Inc.;
  2.
        Based
        on my knowledge, this report does not contain any untrue statement of a material
        fact or omit to state a material fact necessary to make the statements made,
        in
        light of the circumstances under which such statements were made, not misleading
        with respect to the period covered by this report;
  3.
        Based
        on my knowledge, the financial statements, and other financial information
        included in this report, fairly present in all material respects the financial
        condition, results of operations and cash flows of the registrant as of,
        and
        for, the periods presented in this report;
  4.
        I am
        responsible for establishing and maintaining disclosure controls and procedures
        (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) for the registrant
        and I have:
  (a)
        Designed such disclosure controls and procedures, or caused such disclosure
        controls and procedures to be designed under my supervision, to ensure that
        material information relating to the registrant, including its consolidated
        subsidiaries, is made known to me by others within those entities, particularly
        during the period in which this report is being prepared;
  (b)
        Designed such internal control over financial reporting, or caused such internal
        control over financial reporting to be designed under my supervision, to
        provide
        reasonable assurance regarding the reliability of financial reporting and
        the
        preparation of financial statements for external purposes in accordance with
        generally accepted accounting principles;
  (c)
        Evaluated the effectiveness of the registrant’s disclosure controls and
        procedures and presented in this report my conclusions about the effectiveness
        of the disclosure controls and procedures, as of the end of the period covered
        by this report based on such evaluation; and
  (d)
        Disclosed in this report any change in the registrant’s internal control over
        financial reporting that occurred during the registrant’s most recent fiscal
        quarter (the registrant’s fourth fiscal quarter in the case of an annual report)
        that has materially affected, or is reasonably likely to materially affect,
        the
        registrant’s internal control over financial reporting; and
  5.
        I have
        disclosed, based on my most recent evaluation of internal control over financial
        reporting, to the registrant’s auditors and the audit committee of the
        registrant’s board of directors (or persons performing the equivalent
        functions):
  (a)
        All
        significant deficiencies and material weaknesses in the design or operation
        of
        internal control over financial reporting which are reasonably likely to
        adversely affect the registrant’s ability to record, process, summarize and
        report financial information; and
  (b)
        Any
        fraud, whether or not material, that involves management or other employees
        who
        have a significant role in the registrant’s internal control over financial
        reporting.
  Date:  November
                  12, 2008
              By:
              /s/
                  JOHN S. KOVACHJohn.
                  S. KovachChief
                  Executive Officer and Chief Financial Officer(Principal
                  Executive Officer and Principal Financial
                  Officer)