| 1. | I                  have reviewed this Quarterly Report on Form 10-QSB of Lixte Biotechnology                  Holdings, Inc.; |
|----|-------------------------------------------------------------------------------------------------------------------------------|

| 2. | Based                    on my knowledge, this report does not contain any untrue statement                    of                    material fact or omit to state a material fact necessary to make                    the                    statements made, in light of the circumstances under which such                    statements                    were made, not misleading with respect to the period covered                    by this                    report; |
|----|-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| 3. | Based                      on my knowledge, the financial statements, and other financial                      information                      included in this report, fairly present in all                      material respects the financial condition, results of operations                      and cash                      flows of the small business issuer as of, and for, the periods                      presented                      in this report; |
|----|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| 4. | I                        am responsible for establishing and maintaining disclosure                        controls and                        procedures (as defined in Exchange Act Rules 13a-15(e)                        and 15d-15(e)) and internal control over financial reporting                        (as defined                        in Exchange Act Rules 13a-15(f) and 15d-15(f))                        for the small business issuer and                        have: |
|----|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|  | a. | designed                  such disclosure controls and procedures, or caused such disclosure                  controls and procedures to be designed                  under my supervision, to ensure that material information relating                  to the                  small business issuer,  including                  its consolidated subsidiaries, is made known to me by others within                  those                  entities, particularly during                  the period in which this report is being                  prepared; |
|--|----|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|  | b. | omitted; |
|--|----|----------|

|  | c. | evaluated                    the effectiveness of the small business issuer’s disclosure controls and                    procedures and presented in this                    report my conclusions about the effectiveness of the disclosure                    controls                    and procedures, as of the end of the period covered by this report                    based                    on such evaluation; and |
|--|----|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|  | d. | disclosed                      in this report any change in the small business issuer’s internal control                      over financial reporting that occurred during the small business                      issuer’s                      most recent fiscal quarter (the small business issuer’s fourth fiscal                      quarter in the case of an annual report) that has materially                      affected, or                      is reasonably likely to materially affect, the                      small business issuer’s internal control over financial reporting;                      and |
|--|----|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| 5. | I                        have disclosed, based on my most recent evaluation of internal                        control                        over financial reporting, to the small business issuer’s auditors and the                        audit committee of the small business issuer’s board of directors (or                        persons performing the equivalent                        functions): |
|----|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|  | a. | All                          significant deficiencies and material weaknesses in the                          design or                          operation of internal control over financial reporting                          which are                          reasonably likely to adversely affect the small business                          issuer’s ability                          to record, process, summarize and report financial information;                          and |
|--|----|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|  | b. | Any                            fraud, whether or not material, that involves management                            or other                            employees who have a significant role in the                            small business issuer’s internal control over financial                            reporting. |
|--|----|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|                                               |                                                                                                                                                                                  |                                                                                  |
|----------------------------------------------------|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|---------------------------------------------------------------------------------------|
|                                          |                                                                                                                                                                             |                                                                             |
| Date:                            November 13, 2007 | By:                                                                                                                                                                         | /s/ JOHN                          S.                          KOVACH   |
|                                               | John                            S. Kovach                                                                                                                                             |
|                                               | Chief                            Executive Officer and Chief Financial Officer(Principal                            financial and accounting                            officer) |

----