Final Checklist July 9, 2010

Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

(5-day Test)

SECTION A-1. Sample Identifiers

Laboratory Name:

Sampling Episode:

Sample Number or 

Range:

Data Reviewer 

signature:

Date:

Instructions: Complete this checklist electronically or using a ballpoint pen.

Record reviewer comments in the Reviewer Comment section.

Record detailed information in the Comments in the “Overall Assessment” 

section for each criterion not (“N”) met.

Note: Requirements denoted with a * are project requirements that are not 

specific to SM 5210B.

SECTION A-2. OVERALL ASSESSMENT OF THE QUALITY OF THE DATA

General Comment Checklist

Deliverable Requirement

Review 

Level

Required Present Comments

Copies of Shipping Documents (Fed-Ex 

Airbills)

1, 2, 3

Case Narrative 1, 2, 3

Table of Contents 3

Cross reference of Field Sample No., 

Lab Sample No., and Analytical Batch

1, 2, 3

Traffic Report Form (including Sample 

Receipt Checklist)

1, 2, 3

Sample Calculation (usually just a page 

copied from SOW)

3

Results Summary for Each Sample and 

Blank 

1, 2, 3

Field and Laboratory Duplicate Results 2, 3

Water Blank Results 2, 3

Glucose-Glutamic Acid Standard

Results

2, 3

Calibration Check Data 3
Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 2 of 5

Final Checklist July 9, 2010

SECTION A-2. OVERALL ASSESSMENT OF THE QUALITY OF THE DATA

General Comment Checklist

Deliverable Requirement

Review 

Level

Required Present Comments

Copies of Sample Preparation Work 

Sheets

3

Copies of Run Logs 3

Level 1: Review of traffic reports, sample handling, and proper sample preparation

Level 2: Level 1 review plus review of QA/QC results

Level 3: Level 2 review plus review of instrument performance

Level 4: Level 3 review plus analysis of raw data such as chromatograms or primary instrument output
Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

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Final Checklist July 9, 2010

STANDARD METHOD 5210 B Reviewer Comment

Criterion

met

Y N

SECTION B. SAMPLE RECEIPT

1. Reviewed Sample Collection Record: 

• Signatures?

• Completed?

• Sample ID numbers checked?

• Preservations? Temp? Volume?

• Any anomalies reported?

2. Reviewed Lab Sample Receipt Record:

• Date received?

• Lab ID number checked?

• Lab ID number referenced to Sample ID 

numbers?

• Preservation checked by lab? Temp? 

Volume?

• Sample conditions

• Sample hold time

• Any anomalies reported by lab?

SECTION C. SAMPLE PREPARATION/EXTRACT HANDLING 

INFORMATION

1. Reviewed Standard Pre logs: 

• Standard expiration date:

• Concentrations prepared?

2. Reviewed Sample Prep Logs:

• Sample process or run date:

• # of hours between collection and start of 

analysis:

Note: Samples should be analyzed within 24 hours of 

collection. (5210B/1b)

3. *Field Duplicates: Was a field duplicate collected 

and analyzed as part of the sampling episode? 

Indicate the relative percent difference.

SECTION D-1. QC INFORMATION – Method Performance

1. Nitrification Inhibitor: For CBOD5 analysis, was 

nitrification inhibitor added to all samples and 

controls?

If applicable, what nitrification inhibitor was used?

2. Seeding: Were all samples, seed controls, and

glucose-glutamic acid (GGA) for CBOD seeded?
Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 4 of 5

Final Checklist July 9, 2010

STANDARD METHOD 5210 B Reviewer Comment

Criterion

met

Y N

3. Initial Dissolved Oxygen: Was initial DO 

measured (within 30 min) of sample dilution 

preparation? (5210B/4g)

If reported, what was the initial DO measurement?

4. Dilution: If necessary, were enough dilutions 

prepared so that the final result had a residual DO 

≥ 1 mg/L and a DO uptake ≥ 2 mg/L after 5 days 

of incubation? (5210B/4f) 

Note: If there are multiple dilutions that fit the 

above criteria, the average should be used as the 

final result (5210B/5).

5. Are any final DO measurements below 1.0 mg/L?

If so, specify the final DO measurement:

6. Have all samples shown a DO depletion of at least 

2.0 mg/L?

If not, specify the DO depletion:

7. Incubation: Are samples incubated for 5 days ± 6 

hours?

If reported, indicate the incubation time?

8. Seed Correction: Were seed corrections properly 

subtracted from seeded samples?

SECTION D-2. QC INFORMATION – Instrument Calibration

1. Calibration Checks: Was the calibration of the 

probe checked prior to analysis, after every 9 

samples, and at the end of the run?

Was the result within 2% of the true value? If not, 

was the probe recalibrated?

What were the results of the probe checks and the 

percent recovery?

SECTION D-3. QC INFORMATION –Reagent Blanks

1. Water Quality: Was water type or quality 

documented? Is water aged before use, if so how 

long?

2. Was water allowed to come to room temperature 

before usage?

3. Were nutrient and phosphate buffer prepared or 

purchased? 

4. Was reagent quality verified and documented?

5. Phosphate Buffer pH: Was this buffer pH= 7.2?

If not, specify the buffer pH:
Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 5 of 5

Final Checklist July 9, 2010

STANDARD METHOD 5210 B Reviewer Comment

Criterion

met

Y N

6. Water Blank: Was a water blank analyzed in 

each analysis batch? Was it ≤ 0.20 mg/L?

(5210B/4h)

Indicate the water blank reading and any 

contamination noted by the laboratory.

7. Glucose-Glutamic Acid Standards: Was a 

glucose-glutamic acid standard analyzed with each 

analysis batch?

Were results within 198 ± 30.5 mg/L (85-115%)?

What was the percent recovery?

SECTION E. Method QC

1. Laboratory Duplicates: Were duplicates run at 

every 20 samples? (5%) Was the relative percent 

difference less than 20%?

Indicate the results of the sample and the sample 

duplicate and the relative percent difference?

SECTION F. DATA REVIEW

1. Calculations: Was BOD5 calculated using the 

appropriate formula below and reported in 

milligrams per liter (mg/L)? 

For non-seeded samples: P

D1− D2

 

For seeded samples: P

(D1− D2) − (B1− B2) f

Where: D1 = Initial diluted sample DO (mg/L)

D2 = Final diluted sample DO (mg/L)

P = Volumetric fraction of sample used

B1 = Initial seed control DO (mg/L)

B2 = Final seed control DO (mg/L)

f = Ratio of seed in diluted sample to seed 

in seed control