14 MAY 1987

e

One Source.

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u.s. Department of Commerce

National Technical Information Service

OTS0555281

11111111111111 1/111 1/111111111111111111111 1/1111111111111111

INITIAL SUBMISSION: ACUTE INHALATION

TOXICITY OF METHYL IODIDE IN THE RAT WITH

COVER LETTER DATED 08/26/92
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8ECP

8E}{<2~992-10675

09/02192

OTS0555281

88-920008964

EASTMAN KODAK. CO

05/14/87

Microfiche No.

METHYL IODIDE

CODING FORMS FOR SRC INDEXING

Chemical Category

INITIAL SUBMISSION: ACUTE INHALATION TOXICITY OF METHYL IODIDE IN

THE RAT WlTII COVER LETI'ER DATED 08126192
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COMPANYSANmzeD . ORXGINAL PlrBLIC FILE

COpy '1 PUBLIC FILE

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(COMPL:IANCE AUDIT PROGRAM.)

CONTAINS NO cal

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TSCA CONFIDENTIAL

BUS1NESS lNFORWtT1OM

NOn:: Peter provides data eutry :in CBI'rS for 'the BCe) CAP DOCUllleDU.
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Report submitted by: Eastman Kodak Company

343 Stale Street

Rochester, NY 14650

(716) 724-4000

CAP Agreement Ideutifica.:ion NumbeI: (8ECAP~9)

The report penaiDs to iodometbane (synonym: methyl iodide) [CAS # 74-88-4] aad is being

submitted because of histopathologic effectS observed in an acute inhalation study in raIS. The title

ofthe report being submitted is: -Acute Inhalation Toxicity ofMedlyl Iodide in the Rat-. 11Ie

latter report is being identified as a study involving other than human effectS (UDit B.B.2.bofCAP

Agreement).

Groups of 5 male aDd 5 female rats were exposed to 1280, 1600, or 2000 ppm ofmethyl iodide for

one. one hour exposure. All deaths occurred within 48 bouts after exposure. lbe one hoarI.e.

was 1347 ppm and 976 ppm for males aDd females, respectively. Gross abDomIali&ies in aDimals "

dying before study termination included lung hemon:bages, eD1aIged livers, aod pale kidDey

cortices. Bistopalhologic changes in these animals included: irrltation, hemoal1ages, aod fDcal

necrosis oftheorespirarory tract; hydropic degeaeraliOD, focal aec:rosis, aDd. hemoabage ofthe liver;

hydropic degeneration and focal necrosis ofthe reaal tubules; and spleaic lympbal:ic follicle

atrophy. Except for minimal focal iDfIaminariml ofthe lungsin ODe female, DO gross pathologic

cbaDges were observed in animals surviving to study teaDiDaIion.

This miterial is used as an int.emlediate and sold as a pure chemical.

"-=-:;.- ....

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Subject: Report submitted in accordance with guidelines established by the U. S. EnviroDmeDtal

Protection Agency Registration and Agreement for the TSCA 8(e) CompliaDc:e Audit

Program

Dear Sir or Madam:

August 26, 1992

~=in~=n~~~cs 1IIIIIImllili u. S. Enviroameatal Protection Agency I

401 M Street, SW 89928888964

Washington, DC 20460

Attn: section 8(e) CoordiDator (CAP Agreement)

EAS1lwtAN KODAK COMPANY • 343 S'DO'E STREET • ROCHESTER. NEW "19fIJC "14150
230960D

'Ct-86-267

Acut.e Inhalation Toxicity of Ketbyl :Iodide i:l t.he Rat.

Report prepared by:

Eagene R. Kenner. Jr•• B.A.

Toxicological Sciences Sect.iOD

Healt:h and Environment. Laboratories

Eastman ltodak company

2ochest.er••ft 14650

Date of Study COIIIplet.iOD: Kay 14. 1987

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Acute 'Inhalation 'roxieity of 1!ethyl 'Iodide in 1:he Bat.

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Abstract.

Groups of S male aDd 5 f_le rats were exposed to a t:arzet.

concent.rations of 1280. 1600. or 2000 ppm _thyl iodide un)

for one. OIle hour exposure. III caused Saspins. excessive

teariD&. and letharzy in animals exposed to 1280 ppm. ad

S8SPiD&. excessive t.eariD&. usal discurse. cd a sait.

disturbance 111 animals exposed to 1600 or 2000 ppm. Bo deaths

were detect.ed durins t.'ba one hour exp08Ure. .&11 deaths

occurred wit.bin 48 hours after tbe exposure. IIOrtality vas 0.

~o. and 8en. of t.be males and "0. 80. aDd 1~ of the females

for 1280. 1600, cd 2000 ppm. respectivel,... Clinical sips of

toxicity disappeared wit.bin one weele. Body weisbt pin

appeared nomal. except for depressed veiSbt. sain in tbe one

female survivins exposure t.o 1600 ppm. 'rbe probable cau8e of

death for rats dyi.D& after exposure t.o III vas not. det.erained.

Compound-relat.ed sross patholou found in animals dyins before

the scheduled terminat.iOll of t.be study included IUDS

hemorrhases, enlarsed livers. md pale cortex of the 1cidneJ's.

Bo compound-related sross patholozy vas found in ani.Jllals

survivins to the scbeduled termination of the st.udy, except

for one female at 1280 ppm which shoVed minimal focal

infl...t.iOll of the l1Jtl&S. Histopathol~ical expmtnatiOll of

animals which died before the acbeduled necropsy revealed

respirat.ory t.ract irritation. focal necrosis. ed_a aDd

sero-cellular exudate in tbe nasal p".asea. beIIIorrha&e in the

lUl\&s. cd sero-cellular exudate in the trachea. Alao. III

produeed hydropic dese:aeratiOll.focal necroais. _d· beIIIOrrbaSe

of the liver; b1'dropic deseneratiOll aDd focal llecrcMtia of the

renal tubules. cd splenic lymphatic follicle atropbT. 'fbe

nasal passaSes. trachea, IUIIIS, liver. m4 1cidDe7S·are aites

of toxicit.y at. the concentratioaa tested. .a. no-observed-4ffect.

eonc.entration for e1i1dcal sips aDd patholOS7' ... not.

determined. IIOrtalitJ' vas seea. at all CODCeDt.ratioaa t.estad.

'rile ODe hour LC10 was 1347 ppIIl _d 976 ppm for mal.. aDd

f_l88. ~ct.i.ve1Y. ODe hour LC],0 for .-les aa4

females COIIIbined ... 107~ ppID with a 9S'fa cODf'idenee iDtet'ft.1

from 56S t.o 1266 ppm.

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(12-1-81)

..thyl iodide

IodOlMthane

7.-8a-.

C1U

90016.

86-0102

860102:I1

Clt3l:

142

U.S

2.3 at 20120·C

4.9

330 - II: IISDS 8beet.

Chemical ftaIe

Alternate ftaIe

CAS ReI. Bo.

S2ID Bo.

Acc. Bo:

HAEL 80:

Experiment. 80:

8111pirical fo=-ala

1Io1ecu1&r nisht

BoiliD& point. (·C)

Specific Invit.y (H20 • 1)

Vapor deasit.y (Air - 1)

Vapor pre88Ure (torr at a·C)

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Test ProcecSures

Tennessee Eastm:m COlIIPany. tiUS8port.• "Dl

Sponsor

Purpose

Auzgst. 26. 19S6.

study Start Date

Toxicolo&ical Scieftces sectiorl. Health and EnvirOfl2lll!ll'1t. Laboratories.

B-320 Xodalt Park. East.mlm XocSalt Company. Rochest.er. B.Y. 1.650.

Testins Facility

230960D

'1%-86-267

fte purpose of this Rudy was to determine acute toxicity and the LelO

value of methyl io4icSe in t.he rat. folloriD& a _iDele 0Ile hour vapor . exposure.

Acu~e 11Ihalaticm Toxicity of Methyl :Iodide in t.be I1at

This study vas desironed ~o determine the or1e bour LelO valose of methyl

io4ide. :In all o~ber respects. this study was Cor1cSuc~ecS by procedures

similar t.o the Toxic SUbstances Control Act. Test. Cuidelines

(Environmental Prot.ect.iorl qency. September 21. 1985). Guidelines for

Tes~inl of Chemicals: ~03. Acut.e l:nbalatiOl1 Toxicit.,. (orzanizat.icm for

Economic Cooperation and Development.. Ita,.. 1981). mut Annex V.B. 2. Acute

Inbalatiorl Toxicity (Burop8all BCorlO'llliC Coaamity. hptaber. 19&6).

Test Article Characterization

..thyl iodide (1I:I). identified as sam 80. C14&. was obtained froll XocSalt

Laboratory 81:ut Specialty CbtaIIicals. ~ter. 1Ir. ..thyl iodide purit.y

(98.~ - ria las c.broIIIatosrapby/f1alDe icmizatiOll cS-.ctiaa anaiJsia) ....

det.el:'llliuecS 1>1 Chemical8 QualUy senices DivbiOll (CQSD). DcSak Part. JIG

attempt vas _de to identify the t.race COIIIPODeDu 1A ample. ~""..
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E!posu~ Ccrndit.ions

1l.ats were chosen for t.his s1:udy. bec.ause they are a COIIIIIIOIl repreasentative

species for t.oxicity studies. 3:went.y-one male cd twenty-one female

weanliq rats [CRL:coe(SI»Blt] were received from Cbarles tiver

Laboratories. Inc•• Wilmi.Dst.on. ltA.. on Ausust. 20. 1986. 1l.ats ware

selectee! by clinical ex-m;lW.tion. and accordin& to body weisht usiDs the

Auto1llat.ed Animal 'roxicolosy system CULL prosnm. Fifteen 1IIIlle aDd

fifteen female rats weishins from 185-208. and 173-1.93 S1:'IIZIIS

respectively. were select.ed for tbis stud,..

Rousins

fte test. material vapor distribut.ion was measured for 18 case positions

wit.hin inhalation cbamber B-02. and compared t.o a fixed reference

position (Appendix I - Fisure 3). SUbsequently. chamber vapor

concentration was measured from the fixed reference position.

C'bmIlber vapor ccmcentraticrns were determined four t.~ per hour by a

IA infrared analyzer equipped for autQlllated 8ampLiUS and

analysis (Appendix I - PiSUre 2). Cbalber t.empera1:ure and relative

'bumidity was recorded t.wice per hour. and nominal vapor ccrncent.ratioDS

were calculat.ed. 'rWice durins eaeb exposure. the eoncent.rat.ion of t.he

nonsaseous baeql"OU'ft4 material was 1IIe8SUre4 in the exposw:e cbamlnr. and

compared t.o a ncmexposure ebmllber. t.o ccrnfirm that the inhalat.ion

exposures were t.o a vapor. and not an aerosol.

DuriD& the isolation period (five d.,..>. .u.-ls weN srouped boued in

su.,eDded stainless steel c:qes in room 1056. On Allpat 25. 1986. 1:ba

_imals were released to the stud,. in sooc! bea1tb. Du1:'i.1Is lIonexpo.ure

periods. cimals were siDal,. boa.ed in ..ltiCOllParimeD'ted stainless steel

1Ile8h cases in room 1.056. The telllperature cd relative bI.idit,. of room

1056 raused frolll 66 to 73-F. &Cd 56 to 6"'-. reqectivelJ'.

Bale _d female rat.s ware bousec! on the ... rack. A 12 bow:" (6 ..... to

, p •••) p'botoperi04 was maintained. ~orbeAt paper UDder t:bec:qes was

Test. Ani1ll8ls

A complete description of test. mat.erial vapor seneration. samplins. and

maalysis is presented in Appendix I.

llet.hyl iodide :,;.apors were produced by meterins t.he test. material dropwise

into a Slass bead-packed distillins col\llllD supplied with metered. dried.

oil-free compressed air (Appendix 1 - Pi,sure 1).

Betbyl iodide exposures vere conducted in a ..20 L stainless steel and

slasa; inhalat.icm c1ullDber at. tarset. concentratiODS of 2000. 1600. end USO

ppIII.. '.the chamber was maintained at. a lIesat.ive pressure of 0.1 incb water

(sause) relat.ive to t'OOIIl air. and at 6 air chaD&es per bour. For eacb

Srcrup. male and female rats were exposed si.2Dult&neously in the 8l1li8

inhalation cbamber for Ofte hour. Additional animals were exposed to

1600. USO. and on t.he followins day. 800 ppm methyl iodide. for

evaluat.ion of pulmonary function (Experiment 860102P2).
Stat;istical Procedures

230960D

D:-86-267

-3-

Orzans collected at. necropsy for bist.opatbolosic ........inatioa. tnU:'e DUal

passases. tracbea, 1Im&s. aDd abnormal oqaus desipate4 b1' the

pat.bololist.. Abnormal oqaDS collected ineluded eD1ar&ed liven aDd

spleens, kidneys wit.b a pale cortex, aDd bemorrbazic areas of tbe -

stomacb. jejUDUlll.. aDd ileum.

Identification

Clinical Observations

804)" weiSht. vas measured on Days O. 7, aud 14, and cOlllPared to t.beir

initial (Da)" 0) weiSht as a measure of Srovtb. Rats visible t.brouSb

clwDber windows were observed for 'IDOrt.alit.)' cSurins exposure. At. about. 8

a.m. and 3 p.m. on eacb workd8)" during the stud)'. each rat was removed

from its case by a trained technician and observed for clinical -izm; of

toxicity. Observations included. but were not. limit.ed t.o, ex83IIinat.ion of

t.he hair, skin. eyes. mot.or act.ivit.y, feces, and urine.

All randomization and cullin& was achieved b)" computer-senerated list.s

usin& t.he Automated Animal 'toxicology S)"st.em.

Jlandqmizat.ion

Pat.holosy Examinat.ions

Animals d)"in& before t.be scbeduled necro;>8Y dat.e were iJiIDediately

necropsied. SUrvivors were fasted for about 16 hours prior to necropsy

an Day 15. Animals ...re anesthet.ized with CO2 aDd ex:sa:nr;uinated by

severini t.he post.erior vena cava. 'lbe followinz tissues were examined at.

necropsy in all animals: tracbea, l'UD&s, beart. esopbqus. stomach.

duodeDUlll, jejunum. ileum, cecum. colon. paDcreas. liver••ali98%'7 SlaDds.

kidneys, urinary bladder. pituitary Iland. adrenal s1ands, tbJroid

Ilands. parathyroid slands. t.b,..us. spl... _enteric lympb'DOdes. bone

1IIarrOV (femora1.). braiD, t.estes. epidicS)'IIlides. male accesory 11aDds.

ovaries. wlina. uterus. and Fallopian tubes.

certified feed (.I&va)" Prolab Animal Diet mDi-3000. pelleted) and water.

_re available libitUlll only duriD& nonexposure periods. Water vas

available throuSh an IlUtomatic vateriD& system. 'lbe source of the water

was the Ilonroe Count)" wat.er Authorit.y. .0 known contaminants W8re

expect.ed t.o be present in feed or water. which would int.erfere with t.he

outcome of this study.

All rat.s were icSentified by a unique JDel;.al ear t,q.

cbaD&ed tbree times per week. caees and racks were washed once per

week. An;,..ls fram Experiment 860102P2 ...re 'boused in ~e same room with

this study.

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G. btz. Ph.D.

E. Renner• .Jr•• B.A.

R. IIau1.ton

H•. Vlaovic. D.V~H•• 1'b.D.

G. .J. Henkinson. D.V.K•• H.S.

2. Scbumacher. H.S.

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Basalts

Patholosist

Laboratory Animal 1!Iedicitae

Analytic Chemist

Study Director

StueSy 'recllnicians

Exposure COftdiUons

The low temperature of tbe animal bousiUC room ransed fro:n 66 to

72-F. exceecSins tolerances specified in t.he protocol (73 % 3-F) for

the duration of the study.

GLP Statement

Tbechamber flovrate vas 43 liters per miDute. yieldins 6 air chan&es

per hour not 11 as specified in the protocol. because the air

baDcUins system vas modifieeS by addins a vapor scrubbin& system.

The followiD& deviat.ions were observed duriuc the study. but. did not

affect. its outcome:

'1'be final report. data sbeets. aDd all taon-peri.sbable rav dau are stored

in the HAEL archives.

SOP aneS Protocol Deviations

Project Participants

IIe8I1 vapor concentratiODS (~) of t.he tat. ut.erial wre U9M71.

1SS4~162. aDd 1973~4S4 ppm. Exposure CGI:lCeDtratiODS wra comparable

to the tarset CODC8Dtrations. BD4 DOID1Dal CcmCea.t:ratiODS wue 19. 17, cd

2SS biaher tba t.he auJ.yzed vapor CODceDtraUcms. rupect.i~. Overall

-.an ebamber telllperature aDd relative bullidi'ty varied frcra 22 to n-c BD4

63 t.o 7BT.. respectively. Particle COUD't data iD4ica'te4 that _ aertJaol

of the test material Was tao't preseDt. A lIu.ar7 of c"embar espo8UE'8

conditions is presented in'ra1»le 1.

Prior to the study start, c10alLber vapor dist.ribuUon .... det.eal1De4 'by

...surin& the vapo:: CODceIl'traUon at 18 differallt posiUoDa wi'tJain·.

iDhalation cb8lll1Msr 8-02. 'l'be __ CODCeDtratiOll was 394 ppm with. :.>\j~

coefficient of variation of 2S ('fable 'X af -,-"ix 'X). ." . ........"li

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nu study vas conducted .~cordi~ to: 1) Good Laboratory Practice for

Icmclinical Laboratory Studies as prcmulzat.ed by the Food aDd DruS .

Administration. 2:' en Part :58. trecember:' 22. 1978. 2) EIlvironmental

Prot.ection Acency Good Laborat.ory Practice Staftdards "0 CF]l Part. 792.

Iavelllber 29. 1983. except. stability vas not detend.ned. reserve sB1llPles

were not collected. and the desree of absorption vas not determined

because it vas not applicable.

I
Clinical Sisns

230960D

'.1:1:-86-267

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1600 P2!l - Durior. exposure. all anblals exhibited sail. disturbauce

(minor). s&SPins (minor). and excessive teariq (1D04erate). After

exposure. two male rats and t.wo female rats were found dead.

2000 ppm - Durin& exposure. all ani1IIals exhibited saito disturbauce

(millor). letbaru (mocSerate). pspiUS (1Ilinor). and excessive t.eari.n&

(millor). After exposure. t.hree male and five female rats were found dead.

'this cODcentration level was cbDsen durins earl,. t.ecbDical development.

because it was COIIIParable to levels orisinaUy selected for the study

(200. '-00. and 800 pPm). SubsequenUy. these levels were increased to

determine the LC],0 concentration.

~y 'Weisht

Body weiSbt.s are illustrated in FiSUres 1 and 2 for 1II&1es and females.

respectivel,.. 'Individual body weiSbts for Days O. 7. and 1.- are

presented ill -rable XI. Hean body weishts for Da,.s O. 7. and 1'- are sbowD

in 'rable XII. All males surviviD& to the ..d of the auady sbawec!

continued veisbt pins t.brouSh the 14 da,. observation periocS. ~ese

veiSht sains were cOlllParable lIIZIIOD& t.he tbree exposure Sroups. only

female exposed to 1600 pPm survi.viUS t.o the eDc! of t.he auady. exhibUed a

slisht veisht loss on Da,. 7. but subsequently paralleled the weiSht. pin

exhibited by survivors in the 1280 pPm srouP.

IIortality of rats exposed t.o l!I is presented in 'lable 1:V. 110 deat.bs were

found durillS exposure. (Clinical siSns were rat.ed on a four level scale

- mini1llal. millar. 1IIOderat.e. and severe).

'two IDales survived the one bour exposure t.o 2000 ppmKI. One 1II&1e

exhibited poor seneral body con~:'tiOD (minor). S-spiUS (1IliDor). IlAsal

discharse (minor). and die4 within 24 bours followiJl& the exposure.

remainins 'III8le survived to llecropsy. and showed uasal dischar&e (mi'D.imal)

after exposure. but recovered within 2-' bours anc! allPeare!! UOl:'m8l for t.he

rest. of t.he study.

'rbree male and three female rats survived the ODe hour exposure to 1600

ppm KI. 'tWO 1IlBle rats exhibited porphyrin uasal discbaqe (mini1D8l).

ODe of t.hese 1IlBles also shoved sialorrbea ('JIIinor). %be NIIIIlixW:l& 1II8le

rat ex'bibited poor seneral body ccmdiUOD ('JIIinor). All ..lea wrviri.D&

the exposure recovered wit.biJl 24 hourS 8DC! appeared 1lOaaal for t.be rest.

of t.he study.

One female rat exhibited nasal c!iacbar&e (1IIIinor). exc...ive tearinc

(:II';n;-l). and died within 2~ hours followiDs the exposure. bother

f ...le rat showed porphyrin DUal diacbaqe (1D04erate). deUriorat.iD&

Senera1 'body CDDdiUou (1IIoinor prosressiD& to ••vue). excessive t.eacin&

(miniJllal). hypothermia (moderate). C)'aQosis (mi:nor). cad died wltId:D 24

hours followiD& the exposure. 'lbe ODlT female rat. 1lUrri.rin& UIlt1l

'Decropsy sbowd poor seneral body ccmditiOD (mini_]). 'but ncOYM'8d

wit.hiu 24 hours followiD& the exposure. Except for ••Upt. Deck WOUDd.

which healed in ODe week. the female was 1lOrmal for the ~C' of 1:be

study.

"."
PatholoST Examinat.ions

":,.'.i

230960D

T.t-86-267

-6-

Gross Patholop;,r - Tr~.atment-related changes were observed in t.he

l\J1\&s and livers of male and female rats from tbe 2000 and 1600 ppm

exposure sroups. These chan&es were characterized as beJllOrrhaseof t.he

lunss, ancS enlar&ed livers.

The i'robable cause of deat.h for rats dying aft.er exposure t.o met.byl

iodide was not det.ermined. The pat.hologist.'sreport., includin& summary

gross and hiE~opat.holo&y, and individual animal incidence tables, is

presented i':1 Appendix V.

Also seen in males and females of the 2000 ppm exposure &rOUP, was a

t.reatment.-related change t.o the kidneys, described as a pale cortex. 80

t.reatment-relat.ed changes were seen in males of t.he 1280 ppm exposure

&roup.

Rist.opat.bolosy - 'treatment.-related ebanses were seen in usal

passases, lunss, and livers of males and females of the 2000 and 1600 ppm

exposure sroups, and females only from t.he 1280 ppm exposure sroup.

These cban&es were described as the presence of exudate. edema, focal

necrosis. and erosion of t.he nasal passasesi hemorrba&e in the lunss i and

hydropic degeneration and focal necrosis iu the liver.

Clinical signs are summarized in Tables V and VI for males and females,

respectively.

Probit analysis of the data is presented in Appendices II, 111. and IV

for males. females, and combined sexes, respect.ively. "rhe calculated one

hour LC10 was 1347 ppm (95'1. confidence interval = 286 to 1562 ppm) for

males and 976 ppm (upper 95'1. confidenee interval =1206 ppm) for

females. The lower limit of the confidence int.erval can nat. be

calculated for females. because the variance was too hi&b. The one hour

LC10 value for the sexes combined was 1074 ppm (95'1. confidenee interval

= 565 to 1266 ppm). The probability of mortality at. other concentrations can be estimated from Table I in each of Appendices II, 111. and IV.

After the exposure, all female rats exhibited poor &eneral body condition

(minimal). One female rat died within 24 hours following the exposure.

Another female rat showed deterioratiD& body condition (minimal

progressing to moderate). hypothermia (minor), cyanosis (minor), and died

within 48 hours following the exposure. "rhe remaining female rats

recoverecS within 24 hours and appeared normal for the rest of the study.

1.280 ppm - During exposure, all animals exhibit.ed let.bargy (minor),

&aspiD& (mnor). and excessive t.earing (minimal). AJ.l animals survived

to tbe end of t.he exposure.

Five male and five female rats survived tbe one hour exposure t.o 1280 ppm

I!I. After the exposure, all of the male rat.s exhibited poor general body

condition (minimal). one of tbese males also showed excessive tearin&

(minor) . All male rats recovered within 24 bours following t.be exposure

and appeared normal for tbe rest of the study.

---
Discussion

'J:he LelO value for males and females combined is 1074 ppm with a 951.

confidence interval of 565 to 1266 ppm.

2309600

D-86-267

- .. OJ

:::..;..j

,'.: .<_:~L.,,! it.;:,;~~i --- ,~,.

.....

I

-7-

A no-observed-effect level for toxicity was not seen at tbe concentration

levels tested.

Localized toxic effects were seen as irritation t.o t.he respiratory tract

(nasal passages, trachea. and lunss). Systemic effects were observed in

liver. kidneys. and spleen.

Ifethyl iodide is a strong skin irritant whicb is abscrbed t.hrough the

skin (lISDS: 12-1-81.). produci,ns gross edema and skin burns with blist.er

formation. The t.oxic responses observed for methyl iodide would be

expected of a sttong skin irritant. %he mechanism of _tbyl iodide's

toxic action was not determined.

'J:reatment-related changes in kidneys were oberved in two males and one

female of the 2000 ppm group, and described as dilatation. by4ropic

degeneration, and casts in the renal tubule. III the spleen of one 2000

ppm male rat, splenic lymphoid follicles were at.rophied. .

Exposure of rats to vapor concentrations of 1.280. 1600. or 2000 ppm

methyl iodide. produced concentration-dependent clinical signs of

lethargy. gait disturbance. gaspins, excessive tearins. and death.

Also seen were t.reatment-relat.ed cbanses to the tracbeas of males and

females from the 2000 ppm exposure r;roup. and females only in the 1600

ppm exposure r;roup. %bese chan.Zes were seen as sero-cellular exudate in

the trachea.
230960D

n-86-267

/7/,.,t ,,~/?? 7!

Date

Dat.e

5.../1-87

Dat.e

Dat.e

-8-

.~

. . 'n: . " .., ....,d

.....,~ '(".;",: '...".:.,',._.;~?ii;'f.::::iVif:ifi2il.

,;-I' -

Sipature Page

4 mLYR4?rmM

2Ui~ R. Renner J:C. s.l.Q

Inhalation 'tee'hnieian
1

eli

I

..

I

•-

I

,,;

-

I

.;

-

1

!liz

i

1 111

.. 15

tI'I

i

1

.. ,',

........

2309601)

U-a6-267

nGDBE 1

.'ISZ • -.z .'112 " .'~. Cs.MI:!) 'sueJU!' o\IlIlI • '8IOZ .'ga

Body Veiz,ht of Bal.. Jxpo_ed t:o wet-btl 'Iodide

.'l1t

-

%

I!

;:

..

-

i

iii

III

..

•

-

z

!

a:

...

S

•••
I

lit;

!

;

I W

.eIII

i

I

•

I

N

·

<

.':

I

I

....

-

I

.»

-

...

-

.·zzz .'to1Z • 'lIZ '.·Ie1:

· .--__'r"""__.,....__-,-__........__....;...,~;...:;.;..e_~a. .' ,;:j~· ....... ",' ,.,. - • "lIZ

.....,. -,- "

BodY Weicht; of .....1811 '!!posed too ..thy! Xodi4•

23096(1)

'U-86-267

nGUU 2

.... ..

•••

i

..

I

..

ft)

!~~ is ......

a::ft)

......

>u

U
TABLE 'I

..-...

.-" -.~

4

1

2

1

2

22.2:1:0.0

2641

2000

6S.0±0.O

1973:1:454

Study Bo: 86010211

Accession Bo: 900164

Compound 80: 86-0102

2

2

1

22.2:1:0.0

1

1874

1600

63.0±2.8

1554:1:162

2

2

1

1

1472

1280

22.5:1:0.4

77.5:1:0.7

1190±71

1094-1258 1336-1702 1336-2316

lparticle count. data indicat.e4 tbat. an aerosol was 1lOt pnseD.t..

2calculat.ed from daily chemical cOllSUlllPt.iOll an4 total airflow.

STUDY StnD!ARY OF EJPOSUBE COllDI'rIOlllS

230960D

D-86-267

•

Extremes of Daily !leans

•

lIean:l: SD

!leaD: ± SD

Compound: lletbyl iodide

Bumber of Exposures

Analyzed Coneent.ra~ion (ppm)

!lean1± SD

Tat"&e~ COncen~rat.ion (ppm)

blat.ive HumicSit.~ (~)

Temperature (-C)

80111inal Concent.rat.ion (ppm)

lIeanZ
Females

10 16 18

· -{

·-~

· '~

b

b b b

10

202

b 182 b

b

b

2309600

1%-86-267

26 27 27 29 30

16 17 18 19 20

207 212 233

195 192 220 b b

21 22 23 24 2S

177 187 183 197 176

189 :'83 198 184181

186 188 197 192 180

'tABLE II

Individual Animal BodY weigbts and Percent. Weilbt. Gain

b

a Wei&bt. in &rams

b All animals died wit.hin 48 hours aft.ef' exposure. c CWeil,bt. on Dar 14 - Weilht. on Dar 0) x 100

(we1&ht on Day 0)
230960D

D-86-267

WLEnY

Ani'llllll Bean Body lleil'ht.s8

1211._ 1...... ......

PPM .... .... GRCU'-II liIQP-tI GfIIClUJ-II IIEEX , 1

DAY I 1tt.1 33.2 33.1 4.2 11.5 5._ 5 5 5

DAY 7 2M•• 222.7

12.4 b 15.3

wmc. 5 3 , 2

DAY 14 2IS.3 2112•• 12•• 11.4

5 3

1218.... 1....... 2118.... .... .... ..... WEEK GRCU'-F CRCUI-F GIIa.P-F , 1

DAY • 117.2 1U._ 1••1 I .• a.1 1.4 5 5 5

DAY 7 212.2

15.7 e d

3 WEEK , 2

DAY 1. .217.3

13.1

3

• WeiSht. iD &r'8II8.

b ODe male IlUrvived unU1 the .cheduled Decropq. ca DOt: cslcu1ate -.c.

e ODe fema1. 8Urvived UDtil the 8Cheduled DeCrOpq. CIIQ DOt calculate

-.n.

IS All animals died at tbe IQd of exp08Un.

·-. :~

..

".

, ,

.:;
tABLE 1Y

5/5

4/5

2/5

215

.,5

0/5

. Day 14

5/5

4/5

2/5

2/5

0/5

Day 2

2000 pp!!!

1600 pp!!!

1280 pp!!!

SIS

illS

2/5

illS

1/S

OIS

Day 1

5/5

2/5

2/5

o/s

0/5

Post

Exposure

Day 0

Darin&

Exposure

;,-; .....-

;4

:.::;.~~

"L"~b;,.~;.,i.ii~;~fii!il;L;;!'j;tiL~~;~FtI I I

230960D

'%%-86-267

cumulat.ive 1I0rtalit.y of Bat.s Exposed to lIet.lwl Iodide fot" ODe Hour

'1otal ..........••..•..•.••....•....•.••.••...••.•.••••••••••.••••.•• 9110

Females 0/5

Bales 0/5

4f'ot.a.l •••••••••••••••••••• .,.......................................... 6/10

Bales 0/5

Females 0/5

total ••••.••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 2/10

BAles 0/5

Females O/S
TABLE V

2000

11

11-15

11-15

11-15

11-15

11. 12

11. 13-

15

9

8

].600

6-10

6-10

6-10

8. 10

6. 7

23096011

D-B6-267

SU:amary of Clinical Sims - Bales&

Location Clinical Sips 1280b

Bervous

System letbarU 1-5c

sait disturbance

Lunss Supins 1-5

Eyes excessive teariDs 1-5

Body as a

Whole seneral conditiou poor 1-5

IIoutb sialorrhea

Bose porphyrin discharse

dischaqe BaS

deatbd

a·S rats/sroup

b Tarset concentratiou in ppm.

c llulllber assisned to tbe affected rat.

d 110 animal.$ died dUri..ns exposure. Animal Bos. 6. 7. 11. 13-15 died after

exposure.

Lethaqy. sait disturbance. excessive tear1ns. saapias. sialorrhea. aad' nasal

discharse were seec. eiUler durl.ns or just after exposure. 'rbe severity of 'the

clinical sisns vas concentration dependeDt. Abnormal clinical sips resolved

within 48 hours. end did not reappear durinc ~e obaervation period.
26-30

26-30

26-30

2000

26-30

26-30

23

22

24

22

21. 22. 2~. 2S

22.23

22

21-25

21-25

21-25

1600

19

19. 20

16-20

16-20

19

19

16-20

summary of Clinical SilmS - Femalesa

discoloration. blue

exeessive tearins

letbar&Y

sait. 4isturb8Dce

Clinieal Sims

sasping

porpbyrin 4iscbarse

discbarse JIIOS

ceneral condition poor

hypothermia

unkempt haireoat

Body as a

Whole

2309600

D-86-267

., .~~...

Locat.ion

.'_:':

~'1

....." ;J

, . ol' ..

Tail

Bose

Skin of Beck wound

Letbarzy. cait disturbance, excessive teari.Ds. caspius. and DeSal discbaqe

vere seen either darius or just. after exposure. %be "verit.y of ~e clinical

sisns was coneentration dependent. Abnormal clinical sisDs resolved rit.biu .8

hours. and di4 not reappear duriDs the observat.ion period.

a S rats/croup

b Tarset eoncentration in ppm.

C Bumber assisned to t.he affec~ rat..

d 50 animals died duriUS exposure. Animal Bos. 19-22. 2.-30 died after

exposure.