Final Checklist July 9, 2010 

### Steam Study Data Review Checklist

BOD Standard Method 5210 B (1992)

(5

- day Test)

SECTION A

- 1. Sample Identifiers

Laboratory Name:

Sampling Episode: Sample Number or

Range: Data Reviewer

signature: Date:

Instructions: Complete this checklist el ectronically or using a ballpoint pen.

Record reviewer comments in the Reviewer Comment section.

Record detailed information in the Comments in the “Overall Assessment”

section for each criterion not (“N”) met.

Note: R equirements denoted with a * are proje ct requirements that are not

specific to SM 5210B

.



### SECTION A

- 2 . OVERALL ASSESSMENT OF THE QUALITY OF THE DATA

General Comment Checklist

Deliverable Requirement Review

Level

Required Present Comments

Copies of Shipping Documents (Fed

- Ex

Airbills) 1, 2, 3

Case Narrative 1, 2, 3

Table of Contents 3

Cross reference of Field Sample No.,

Lab Sample No., and Analytical Batch 1, 2, 3

Traffic Report Form (including Sample

Receipt Checklist) 1, 2, 3

Sample Calculation (usually just a page

copied f rom SOW) 3

Results Summary for Each Sample and

Blank 1, 2, 3

Field and Laboratory Duplicate Results 2, 3

Water Blank Results 2, 3

Glucose

- G lutamic Acid Standard

Results 2, 3

Calibration Check Data 3Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 2 of 5

Final Checklist July 9, 2010 

### SECTION A

- 2 . OVERALL ASSESSMENT OF THE QUALITY OF THE DATA

General Comment Checklist

Deliverable Requirement Review

Level

Required Present Comments

Copies of Sample Preparation Work

Shee ts 3

Copies of Run Logs 3

Level 1: Review of traffic reports, sample handling, and proper sample preparation

Level 2: Level 1 review plus review of QA/QC results

Level 3: Level 2 review plus review of instrument performance

Level 4: Level 3 review pl us analysis of raw data such as chromatograms or primary instrument outputSteam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 3 of 5

Final Checklist July 9, 2010 

### STANDARD METHOD 5210 B Reviewer Comment Criterion

met

Y N

SECTION B . SAMPLE RECEIPT

1. Reviewed Sample Collection Record:

**•** Signatures?

**•** Completed?

**•** Sample ID numbers checked?

**•** Pr eservations? Temp? Volume?

**•** Any anomalies reported?

2. Reviewed Lab Sample Receipt Record:

**•** Date received?

**•** Lab ID number checked?

**•** Lab ID number referenced to Sample ID

numbers?

**•** Preservation checked by lab? Temp?

Volume?

**•** Sample conditions

**•** Sample hold time

**•** A ny anomalies reported by lab?



### SECTION C . SAMPLE PREPARATION/EXTRACT HANDLING

INFORMATION

1. Reviewed Standard Pre logs:

**•** Standard expiration date:

**•** Concentrations prepared?

2. Reviewed Sample Prep Logs:

**•** Sample process or run date:

**•** # of hours between coll ection and start of

analysis:

Note: Samples should be analyzed within 24 hours of

collection. (5210B/1b)

3. *Field Duplicates: Was a field duplicate collected

and analyzed as part of the sampling episode?

Indicate the relative percent difference.



### SEC TION D

- 1. QC INFORMATION

– Method Performance

1. Nitrification Inhibitor: For CBOD 5 analysis, w as

nitrification inhibitor added to all samples and

controls?

If applicable, what nitrification inhibitor was used?

2. Seeding: Were all samples, seed contro ls, and

glucose

- glutamic acid ( GGA ) for CBOD seeded?Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 4 of 5

Final Checklist July 9, 2010 

### STANDARD METHOD 5210 B Reviewer Comment Criterion

met

Y N

3. Initial Dissolved Oxygen: Was initial DO

measured (within 30 min) of sample dilution

preparation? (5210B/4g)

If reported, what was the initial DO measurement?

4. Dilution: If necessary, were enough dilutions

prepared so that the final result had a residua l DO

≥ 1 mg/L and a DO uptake ≥ 2 mg/L after 5 days

of incubation? (5210B/4f)

Note: If there are multiple dilutions that fit the

above criteria, the average should be used as the

final result (5210 B/5).

5. Are any final DO measurements below 1.0 mg/L ?

If so, specify the final DO measurement:

6. Have all samples shown a DO depletion of at least

2.0 mg/L?

If not, specify the DO depletion:

7. Incubation: Are samples incubated for 5 days ± 6

hours?

If reported, indicate the incubation time?

8. Seed Correction: Were seed corrections properly

subtracted from seeded samples?



### SECTION D

- 2. QC INFORMATION

– Instrument Calibration

1. Calibration Checks : Was the calibration of the

probe checked prior to a nalysis , after every 9

samples, and at the end of the run ?

W as the result within 2% of the true value ? If not,

was the probe recalibrated?

What were the result s of the probe checks and the

percent recovery?



### SECTION D

- 3. QC INFORMATION

– Reagent Blanks

1. Water Quality : Was water type or quality

documented? Is water aged before use, if so how

long?

2. Was water allowed to come to room temperature

before usage?

3. Were nutrient and phosphate buffer prepared or

purchased?

4. Was reagent quality verified and documented?

5. Phosphate Buffer pH: Was this buffer pH= 7.2?

If not, specify the buffer pH:Steam Study Data Review Checklist

BOD Standard Method 5210B (1992)

Page 5 of 5

Final Checklist July 9, 2010 

### STANDARD METHOD 5210 B Reviewer Comment Criterion

met

Y N

6. Water Blank: Was a water blank analyzed in

each analysis batch? Was it ≤ 0.20 mg/L?

(5210B/4h)

Indicate the water blank reading and a ny

contamination no ted by the laboratory.

7. Glucose

- G lutamic Acid Standards : Was a

glucose

- glutamic acid standard analyzed with each

analysis batch?

Were results within 198 ± 30.5 mg/L (85

- 115%) ?

What was the percent recovery?



### SECTION E

. Method QC

1. Laboratory Duplica tes: Were duplicates run at

every 20 samples? (5%) Was the relative percent

difference less than 2 0%?

Indicate the result s of the sample and the sample

duplicate and the relative percent difference?



### SECTION F

. DATA REVIEW

1. Calculations: Was BOD 5 calcu lated using the

appropriate formula below and reported in

milligrams per liter (mg/L)?

For non

- seeded samples:

P D D 2

1

**−**

For seeded samples:

P f B B D D ) 2 1 ( ) 2 1 (

**− −**  **−**

W here: D1 = I nitial diluted sample DO (mg/L)

D2 = Final diluted sample DO (mg/L)

P = Volumetric fraction of sample used

B1 = Initial seed control DO (mg/L)

B2 = Final seed control DO (mg/L)

f = R atio of seed in diluted sample to seed

in seed control