UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON, D.C. 20460 

OFFICE OF CHEMICAL SAFETY AND 

POLLUTION PREVENTION 

MEMORANDUM 

Date: 6/29/2015 

SUBJECT: Atrazine - Amphibian Metamorphosis Assay 

PC Code: 0808803 

Decision No.: 

Petition No.: NA 

Risk Assessment Type: NA 

TXR No.: 0057284 

MRID No.: 48614801 

DP Barcode: D399650 

Registration No.: NA 

Regulatory Action: NA 

Case No.: NA 

CAS No.: 27314-13-2 

40 CFR: NA 

FROM: Greg Akerman 'J-.c.~<2-A- ~-< ~ Immediate Office 

Health Effects Division (7509P) 

THROUGH: Jess Rowland '6c-~--~s;;> __ Deputy Director 

Health Effects Division 

TO: Jolene Trujillo 

Biologist/Chemical Review Manager 

Risk Management and Implementation Branch V 

Pesticide Re-evaluation Division (7505P) 

CONCLUSION 

A Data Evaluation Record (DER) for this study is attached. 

Ver.Apr. 2010 

CITATION: Wolf, J.C. 2012. Atrazine: Study to Satisfy the EPA Test Order # EDSP-080803-

1,2,3,4 Guideline Study 890. 11 00 Amphibian Metamorphosis Assay (AMA) Data Requirement for 

Atrazine Using Existing Frog Tissues Available from Kloas et al. Studies. Unpublished study 

performed by Experimental Pathology Laboratories, Inc., Sterling, VA. Laboratory report number 

140-121. Study sponsored by Syngenta Crop Protection, LLC, Greensboro, NC. Study completed 

February I 0, 2012. 

Page I of2 

Page 1 of 59
Page 2 of 2 

The objective of the current study was to evaluate preserved tissues from Xenopus laevis frogs 

for potential effects of atrazine on thyroid-dependent endpoints, in response to a test order for an 

Amphibian Metamorphosis Assay (OCSPP 890.1100). The frogs in this study were archived 

specimens, preserved at Nieuwkoop and Faber (NF) stage 66 (complete metamorphosis, 43-75 

days post fertilization), that were originally utilized for gonad histopathology investigations. 

Specimens (100 frogs from each treatment and control) were evaluated from the original 

treatment groups of 0 (negative control), 0.0010, 0.025, and 0.100 mg a.i./L. The test system in 

the original study was conducted under flow-through conditions and was maintained at 22±1°C. 

As this study was originally conducted under a different protocol than the 890.1100 and the data 

report submitted under MRIDs 47046201-4 and 47153501 , there are differences in the collection 

of data and reporting endpoints. The differences are outlined below in the Methods and Material 

section under Guideline followed. Prior to submission of these additional thyroid-dependent 

endpoints, the registrant contacted the Agency to requesting to use frogs from the previous assay 

for evaluating the thyroid-dependent endpoints.

There were no significant effects (p>0.05) detected on snout-to-vent length or dry weight 

endpoints in the original study. No effects were seen on time to metamorphosis (p>0.05), but 

results were not reported for effects on median Nieuwkoop-Faber (NF) developmental stage 

because this parameter was not relevant to the original, stage-matched experimental design. No 

developmental abnormalities were reported, although asynchronous development was not 

specifically observed. Thyroid histological findings in preserved NF stage 66 specimens included 

follicular cell hypertrophy, follicular cell hyperplasia, and decreased colloid, but there was no 

indication that these findings were more severe or occurring with greater incidence in the 

atrazine-treated groups, relative to the control group. Other histological endpoints such as 

changes in thyroid follicular lumen, cell height, or shape were not reported. Atrazine exposure 

was slightly (3-5%), but significantly (p<0.05), associated with greater normalized hind-limb 

length (HLL) values in the NF stage 66 frogs at all treated concentrations, relative to the control. 

When considered in conjunction with other Tier 1 EDSP data available for atrazine, this 

submission satisfies the Test Order requirement for an Amphibian Metamorphosis Assay 

(OCSPP Guideline 890.1100). 

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 EPA MRID Number 48614801

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DER Template Version: 22 September 2011 

   
     
    	 

 EPA DP Barcode D399657 

OECD Data Point 231 

EPA MRID 48614801

EPA Guideline 890.1100 

 Amphibian Metamorphosis Assay (Frog) 

      
    

Atrazine 

!  
   " # $ 

 Not Reported in this MRID, but was 97.1% in original report 

Common Name : Atrazine 

Chemical Name IUPAC Not Reported 

 CAS Name Not Reported 

CAS No. Not Reported 

Synonyms None Reported

EPA PC Code 080803

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John Marton, Ph.D. 

   

 5/9/2012 

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Teri S. Myers, Ph.D.    6/30/2012

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: Amy Blankinship   : 

EPA/OPP/EFED/ERB3 

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Catherine Aubee    6/12/2015

EPA/OPP/EFED/ERB4 

           	 '        

6/12/15 

AMY BLANKINSHIP

Digitally signed by AMY BLANKINSHIP 

DN: c=US, o=U.S. Government, 

ou=USEPA, ou=Staff, cn=AMY 

BLANKINSHIP, 

dnQualifier=0000040917 

Date: 2015.06.15 05:44:47 -04'00'

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 EPA MRID Number 48614801

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DER Template Version: 22 September 2011 

' )    ) , - 

 Wolf, J.C. 2012. Atrazine: Study to Satisfy the EPA Test Order # EDSP-080803-1,2,3,4 Guideline 

Study 890.1100 Amphibian Metamorphosis Assay (AMA) Data Requirement for Atrazine Using Existing Frog 

Tissues Available from Kloas et al. Studies. Unpublished study performed by Experimental Pathology 

Laboratories, Inc., Sterling, VA. Laboratory report number 140-121. Study sponsored by Syngenta Crop 

Protection, LLC, Greensboro, NC. Study completed February 10, 2012. 

Note: The US EPA Endocrine Disruptor Screening Program (EDSP) Tier 1 screening battery is comprised of eleven 

screening assays intended to identify a chemical’s likely endocrine bioactivity, i.e., its potential to interact with the 

estrogen, androgen, or thyroid (E, A, or T) pathways. The robustness of the Tier 1 battery is based on the strengths 

of each individual assay to identify potential endocrine bioactivity with complementary endpoints within the assay, 

where available, and redundancy across the battery. Thus, the results of each individual assay should not be 

considered in isolation but rather should be considered in the context of other assays in the battery as well as 

Other Scientifically Relevant Information (OSRI). In order to determine if a chemical has the potential to interact 

with the E, A or T pathways, a Weight of Evidence (WoE) evaluation of Tier 1 assay results, in combination with 

the findings in the OSRI, should be undertaken (refer to the WoE Document). 

. / 0 1 2 3 / 4 5 6 7

The guideline recommendations in this DER template are offered as a general reference to aid 

in preparation of the DER. The purpose of these recommendations is not to serve as substitute for the Test 

Guidelines, nor to provide any guidance on how the study should be conducted.

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 EPA MRID Number 48614801

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DER Template Version: 22 September 2011 

 9  ' :  ) ;  & :    
 <

The objective of the current study was to evaluate preserved tissues from Xenopus laevis frogs for potential effects 

of atrazine on thyroid-dependent endpoints, in response to a test order for an Amphibian Metamorphosis Assay 

(OCSPP 890.1100). The frogs in this study were archived specimens, preserved at Nieuwkoop and Faber (NF) 

stage 66 (complete metamorphosis, 43-75 days post fertilization), that were originally utilized for gonad 

histopathology investigations. Specimens (100 frogs from each treatment and control) were evaluated from the 

original treatment groups of 0 (negative control), 0.0010, 0.025, and 0.100 mg a.i./L. The test system in the 

original study was conducted under flow-through conditions and was maintained at 22±1°C. As this study was 

originally conducted under a different protocol than the 890.1100 and the data report submitted under MRIDs 

47046201-4 and 47153501 , there are differences in the collection of data and reporting endpoints. The 

differences are outlined below in the Methods and Material section under Guideline followed. Prior to submission 

of these additional thyroid-dependent endpoints, the registrant contacted the Agency to requesting to use frogs 

from the previous assay for evaluating the thyroid-dependent endpoints. 

There were no significant effects (p>0.05) detected on snout-to-vent length or dry weight endpoints in the original 

study. No effects were seen on time to metamorphosis (p>0.05), but results were not reported for effects on 

median Nieuwkoop-Faber (NF) developmental stage because this parameter was not relevant to the original, 

stage-matched experimental design. No developmental abnormalities were reported, although asynchronous 

development was not specifically observed. Thyroid histological findings in preserved NF stage 66 specimens 

included follicular cell hypertrophy, follicular cell hyperplasia, and decreased colloid, but there was no indication 

that these findings were more severe or occurring with greater incidence in the atrazine-treated groups, relative 

to the control group. Other histological endpoints such as changes in thyroid follicular lumen, cell height, or shape 

were not reported. Atrazine exposure was slightly (3-5%), but significantly (p<0.05), associated with greater 

normalized hind-limb length (HLL) values in the NF stage 66 frogs at all treated concentrations, relative to the 

control. 

When considered in conjunction with other Tier 1 EDSP data available for atrazine, this submission satisfies the 

Test Order requirement for an Amphibian Metamorphosis Assay (OCSPP Guideline 890.1100). 

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      & 	      

Test organism NF stage at test initiation: Not Reported [reported as NF stage 46-48 in original study report, 

8 dpf] 

Test organism total length at test initiation (optional): Not Reported 

Test type: flow-through 

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Abbreviations: Diff. Difference. NA Not applicable. DPF Days post fertilization.

1

 A “yes” indicates a significant difference based on comparison to the negative (clean water) control, unless 

otherwise specified. 

2

 The criteria for significance are described in the Reviewer’s Analysis and Statistical Verification sections of the 

DER. Conclusions regarding histopathology may be heavily weighted by the expert opinion of a board-certified 

pathologist. 

3

 Hind-limb length is normalized to snout-vent length (SVL). 

4

 Frogs were binned by sacrifice date, between days 43 and 75 post-fertilization (dpf). 

 
     	 

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0.0010 NA NA NA Yes NA NA No 

0.025 NA NA NA Yes NA NA No 

0.100 NA NA NA Yes NA NA No 

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G H I J K L M G J N O J P Q I L K R S Q O

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 This study was not conducted following OCSPP 890.1100. Rather, preserved 

specimens from a previous study on the effects of atrazine on the gonad 

histopathology of Xenopus laevis tadpoles were analyzed for thyroid pathology 

and growth parameters (hind-limb length, snout-vent length, and wet weight) 

were measured by image analysis. All pertinent details about the conduct of 

the original study were not included in this report, however the details of many 

of the identified deviations below were discussed in the original report (MRID 

47153501). The following deviations from OCSPP 890.1100 were noted: 

1. Based on the report, the left hind limb was not always measured as in some cases it was not available 

for analysis, and in cases were both left and right were available a coin toss was used to determine 

which one was measured. 

2. The percent mortality in one replicate of the negative control was 20% (5 tadpoles out of 25 exposed) 

and was greater than the 10% recommended in the 890.1100 guideline. 

3. NF stage at initiation of the original study was not reported. However, it was specified that tadpoles 

were 8 days post fertilization at test initiation and the original report reported stage at NF 46-48 at 

initiation. Further, NF stage was not recorded at any point throughout the original study. 

4. The purity, description, lot/batch number and associated impurities of the test material were not 

reported in this report. Purity reported as 97.1% atrazine in original report. 

5. Stability of the test material was not reported. Only nominal concentrations were reported. Test 

concentrations were measured and results reported in original report. 

6. Larval selection methods for use in the definitive test, were not specified. In original report, ten pairs 

of adult frogs used for embryo selection and larvae were combined during acclimation for eventual 

exposure. 

7. While detailed descriptions of the type of flow-through system, or source of dilution water (or 

contaminant screen) were not reported in this study, these details were presented in the original 

report. 

8. Supplemental aeration was provided to the test vessels, though the aeration rate was not reported. 

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9. Hardness (90-130 mg/L as CaCO3) and pH (7.9-8.3) of the dilution water exceeded the 

recommended ranges (40-48 mg/L as CaCO3, 7.5 ± 0.1). Actual temperature and DO 

measurements were not specified. 

10. Original study was conducted for 75 days (8-83 dpf). In original reported tested from 8 to 83 dpf 

or until frogs reached NF stage 66. 

11. The reported light intensity (0.1-0.5 Klux) was lower than recommended (0.6-2 Klux). 

12. While in this study, the study authors did not specify when SVL and wet weight measurements were 

taken in the original study. However, in the original report, SVL and wet weight were measured after 

frogs reached stage 66. 

The original study was conducted in accordance to its specific protocol and was determined to be scientifically 

valid. The results in the current submission provide additional information regarding amphibian larval development 

and growth through metamorphosis. 

'       	   

 Signed and dated No Data Confidentiality, GLP, and Quality Assurance statements 

were provided. This study was conducted in compliance with the Good 

Laboratory Practice Regulations of the Environmental Protection Agency 

(FIFRA) as stipulated by 40 CFR Part 160; and all applicable amendments. 

 *       
   

 Atrazine 

    
     	 

Not Reported

OECD recommends describing water solubility, melting/boiling point stability in water and light, pKa, Pow or Kow, 

vapor pressure of test compound, expiration date.

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 Not Reported 

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 Not Reported 

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Not specified. Only nominal concentrations were reported. The concentrations of 

atrazine did decline during the first part of the test and as animals completed 

metamorphosis and were removed from the tanks, the measured 

concentrations increased and better approximated nominal concentrations. The 

stocks were measured and were consistent with nominal concentrations and the 

diluter system was monitored, therefore it was thought that the loss of atrazine 

in the system was due to uptake in the animals.

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 Not specified 

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 EPA MRID Number 48614801

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in original study report (MRID 47153501).

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Species common name: Xenopus laevis EPA recommends African clawed frog 

(Xenopus laevis). Western [Africa] clawed 

frog Silurana (Xenopus) tropicalis may be 

used as an alternate species1; however, a list 

of all of the necessary protocol deviations to 

accommodate this species is recommended 

for inclusion in the study report. The guideline 

recommends that the performance criteria 

used to support the reliability of the test be 

identified.

Species scientific name: African clawed frogSpecies strain (if stated): Not reported 

1 U.S. Environmental Protection Agency (EPA). (2011). Corrections and Clarifications on Technical Aspects of the Test Guidelines for the Endocrine Disruptor 

Screening Program Tier 1 Assays (OCSPP Test Guideline Series 890). March 3, 2011. Office of Chemical Safety and Pollution Prevention (OCSPP), Washington, D.C. (http://www.epa.gov/endo/pubs/assayvalidation/clarificationdoc.pdf). 

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Were parents maintained as in-house stock? 

No EPA recommends that larvae used in the 

assay be derived from in-house adults.

Were parental acclimation conditions same as definitive test? 

Not specified Culture conditions reported in original 

report

Acclimation period for parental 

frogs (if applicable):

Not specified Tadpoles were received 3 days 

post-fertilization (dpf) and were acclimated during dpf 4-7. 

Details on parental feeding: None provided Details on parental health: None provided 

f W n[ a  z  Wc Z W [} a [ ab X

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Best single spawn? Not specified EPA and OECD recommend that the best 2 – 3 individual spawns, with a minimum of 1500 Number of spawns evaluated (if Not specified Eggs collected from 10 pairs of adults and 

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applicable): embryos combined in original report larvae/spawn, be evaluated to identify the 

best single spawn, and that the larvae selected 

for testing originate from the best single spawn 

(i.e., the spawns are not co-mixed)

Number of eggs sampled per spawn:

Not specified 

NF stage at test initiation Not reported In original report, stage reported as 46-48 

at initiation 

EPA recommends that the definitive study be 

initiated with larvae at Nieuwkoop – Faber 

(NF) developmental stage 51 (  17 days 

post-fertilization).

Age at test initiation: 8 days post-fertilization 

(dpf) 

Mean total length at test initiation (if reported): 

Not reported

Range of total length at test initiation (if reported): 

Not reported 

Was the optional size selection method used? 

Not specified 

Details on larval selection: None provided Loading rate (rearing density): ~3.6 larvae/L 25 larvae per tank (7 L) EPA recommends that rearing density 

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(loading rate) not exceed approximately 10 

larvae/L culturing system for flow-through 

systems or 4 tadpoles/L in static-renewal 

exposure systems.

Type of food: Sera Micron® EPA recommends Sera Micron® throughout 

pre-exposure (after NF stage 45/46) and 

during the entire 21-d definitive study. If 

another diet is used, the study report should 

provide analysis of iodide content and potential 

contaminants, and the diet should 

demonstrate equal performance to Sera 

Micron® .

Source of food: Sera MicronIodide concentration in diet (if known):

Not reported 

Frequency of feeding: three times/day EPA recommends that feeding occur at least 

twice per day.

Details on feeding regime: 300 mg/tank at 8 dpf, 

increasing to 900 mg/tank at 33 dpf, then 

There were 25 tadpoles/tank at test initiation.

It is recommended that food rations during the 

pre-exposure period be increased along with 

larval growth to approximately 30 

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adjusted downward proportionately as frogs were removed from the tanks.

mg/larva/day by test initiation. EPA and 

OECD recommend that food rations increase 

from 30 mg/larva/day at test initiation (Study 

Day 0-4) to 80 mg/larva/day in the last week 

of the test (Study Day 15-21).

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f W n[ a  z } \ llWc h ^ i| _ i ^clW X] ^_^_ Xe a Y g m^o \ ca } h o X alW_ df ao X aooa [e WcWb X ac ] o X] bo . Again, details about exposure provided in original report 

(MRID 47153501)

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Type of exposure: Flow-through EPA recommends the use of a flow-through 

system.

Type of flow-through dilution system (if applicable):

Not reported Intermittent flow proportional diluters or 

continuous flow serial diluters are 

recommended.2

Flow-through rate (if applicable):

~35 mL/min Calculated from tank volume (7 L) and daily 

flow rate (50 L/day).

Recommended flow-through rate is 25 mL/min (complete volume replacement ca. 

every 2.7 hrs).

Details on toxicant mixing for flow-through systems (if applicable):

None provided Recommended toxicant mixing for 

flow-through systems: 1) Mixing chamber is 

recommended but not required; 2) Aeration 

is not recommended for mixing;3) A 

2 Additional guidance for aquatic test design is located in OCSPP Guideline 850.1000, Special Considerations for Conducting Aquatic Laboratory Studies. 

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demonstration that the test solution is 

completely mixed before introduced into the 

test system is recommended; 4) The 

recommended flow splitting accuracy is 

within 10%.

Renewal period for static renewal (if applicable):

N/A- test was conducted under flow-through conditions 

If static renewal is used, EPA recommends 

24-hr renewal; renewal period is 

recommended not to exceed 72 hours.

Aeration? Yes; rate not reported EPA recommends maintaining dissolved 

oxygen concentrations >40% air saturation 

(>3.5 mg/L). Aeration may be maintained through bubblers. It is recommended to set 

bubblers at levels that do not cause stress 

on the tadpoles.

Source of dilution water: Not specified. Water was 

filtered (0.45 μm), UV-sterilized, and 

EPA recommends natural or reconstituted 

water; it is recommended that natural water 

be sterilized with UV and tested for 

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screened for contaminants. 

pesticides, heavy metals, and other 

possible contaminants, including known 

substrates of the iodine transporter of the 

thyroid gland (e.g., fluoride, chlorate, 

perchlorate). OECD accepts any water in 

which the test species show control survival 

at least as good as indicated in the test 

guideline.

Was dilution water analyzed for pesticides, heavy metals, and other contaminants? 

Yes. Results not provided. 

Iodide supplementation in water?

Not specified If reconstituted water is used or if 

background levels of iodide in natural water 

are less than 0.5 μg/L, iodide 

supplementation is recommended. This 

supplementation is in addition to the 

recommended dietary source of iodide (e.g.. in Sera Micron).

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Test vessel type/materials: Not specified EPA and OECD recommend that 

water-contact portions of the system not 

compromise the study (e.g., all glass 

vessels or glass vessels with stainless steel 

frames are acceptable examples).

Test vessel size: Not reportedFill volume: 7 L

Additional details on exposure 

system: 

None provided 

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Hardness (mg/L as CaCO3) 90 130 Not Reported Not Reported 

EPA recommends hardness 40 to 48 

mg/L as CaCO3.

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pH 7.9 8.3 Not Reported Not Reported 

EPA recommends pH 7.5 ± 1, 

inter-replicate and inter-treatment 

differentials should not exceed 0.5.

Dissolved oxygen (mg/L) -- --  60% Not Reported 

EPA recommends dissolved oxygen (DO) 

>3.5 mg/L (>40% air saturation). OECD 

recommends DO concentration >3.5 mg/L

(>40% air saturation).

Temperature (°C) -- -- 22±1 Not Reported 

EPA recommends temperature 22±1oC; 

inter-replicate and inter-treatment 

differentials should not exceed 0.5oC.

Iodide Not Reported 

EPA recommends aquatic iodide range 0.5 – 10 μg/L (supplemental iodide should not 

exceed 2 μg/L).

Ammonia (mg/L) 0.0 0.35 Not Reported Not Reported General recommendations for frequency of 

measurements: EPA recommends that 

water quality parameters be measured in a 

control and at one test item concentration 

at least weekly. In static renewal systems, 

Fluoride Not Reported Perchlorate Not Reported Chlorate Not Reported 

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Nitrate (mg/L) 

Alkalinity (mg/L as CaCO3) 

Conductivity (μS/cm) 

0.04 

133 

726 

2.29 

172 817 

Not Reported Not Reported 

water quality parameters, including 

ammonia, should be measured just prior to 

renewal. In addition, EPA recommends that 

DO be measured at each concentration at 

least weekly and that temperature be measured continuously. OECD 

recommends that DO and temperature be 

measured at least weekly and that pH and 

hardness be measured at least at the 

beginning and end of the test.

V v }X \ d h V ao ] x_W_ djdd]X

] ^_W [Y g mac ] la_ X W [ ^_ d]X] ^_o

f W n[ a  z ` W_xa  ] _ d] _x }X \ d h  ^_ d]X] ^_o ]ij mm [] bW n[ a  v

~ WcWla X ac  W [ \ a  o V a X W ][ o^c

` alWc  o { \ ]d a [] _a

` ab^lla_ d W X] ^_o

Was a range-finder conducted? Not reportedIf yes, what was the method for N/A The study from which preserved EPA recommends that the highest test 

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determining the highest test concentration in the range-finder? 

specimens were collected included nominal concentrations of 0.01, 0.1, 1.0, 25, and 100 μg/L. The three highest treatment groups were selected for analysis in the current study. 

concentration is either the solubility limit of the 

test compound, 100 mg/L, or demonstrates 

adequate evidence of toxicity (e.g., <10% 

mortality), whichever concentration is lowest.

Species: Xenopus laevis Life stage: Not Reported Test duration: Not Reported

Additional details: Tadpoles used in the 

current study were archived specimens that were originally utilized for gonad histopathology investigations of atrazine as reported in Kloas et al. 2008. 

Original test was conducted from 8 dpf until complete metamorphosis (83 dpf). Defined as NF 46-48 at initiation to NF 

stage 66 in original report.

Page 21 of 59
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f W n[ a t z V a i] _ ]X] Z a }X \ d h  ^_ d]X

] ^_o v Details about study conduct were submitted under MRID 47153501

~ WcWla X ac  W [ \ a  o V a X W ][ o^c

` alWc  o { \ ]d a [] _a

` ab^lla_ d W X] ^_o

Test duration: 75 days (original study) EPA recommends that the duration of the 

definitive test be 21 days. 

Method for selecting the highest test concentration in the definitive test:

Not reported EPA recommends that the highest test 

concentration is either the solubility limit of the 

test compound, 100 mg/L, or demonstrates 

adequate evidence of toxicity (e.g., <10% 

mortality), whichever concentration is lowest.

Reference study citation (if applicable):

Hosmer, A, T Springer, W Kloas, I Lutz, J Wolf, and L Holden (2007) Response of Larval Xenopus laevis to 

Atrazine Exposure: Assessment of 

Metamorphosis and Gonadal Morphology: Overview and 

Data from the original study also appeared in Kloas, W, I Lutz, T Springer, H Krueger, J Wolf, L Holden, and A Hosmer (2009) Does atrazine influence larval development and sexual differentiation in Xenopus laevis? Toxicological Sciences, 

107(2): 376-384. Data also submitted under MRID 47153501.

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~ WcWla X ac  W [ \ a  o V a X W ][ o^c

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Comparison of Sudies. Response to Atrazine Generic and Product Specific Data Call-In Notice, March 17, 2004. Syngenta Study Number T002536-05. 

Separation of test concentrations: 0.04-0.25 EPA recommends that the maximum 

concentration separation be 0.1 and the 

minimum be 0.33. 

Number of test concentrations: 3 EPA recommends a minimum of 3 

concentrations and a control, plus solvent 

control if appropriate.

Are nominal concentrations 

adjusted for purity? 

Not reported 

Indicate the type of values presented for measured 

No measured concentrations were 

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concentrations: reported. Limit of quantification (LOQ): Not reported EPA recommends that for chemical test 

concentrations below the LOQ, analyses be conducted on the stock solutions.

Level of detection (LOD): Not reported Frequency of measurement: Not reported It is recommended that test item concentration 

be measured in one tank at each treatment 

level at test initiation and every week 

thereafter.

Number of replicates in control: 16 replicate tanks in 4 

clusters

One cluster was randomly chosen so that 4 replicates (maximum 25 tadpoles/rep) were selected for image analysis and thyroid histopathology. 

EPA recommends 4 replicates.

Number of replicates in solvent control (if applicable):

N/A; a solvent was not used

EPA and OECD recommend the use of a 

concurrent solvent control when a solubilizing 

agent is used. EPA recommends 4 replicates.

Page 24 of 59
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Number of replicates per test item treatment level: 

8 replicate tanks, 2 clusters for each group 

One cluster was randomly chosen so that 4 replicates (maximum 25 tadpoles/rep) were selected for image analysis and thyroid histopathology. 

EPA recommends 4 replicates.

Number of larvae per treatment at test initiation:

400 larvae in negative control, 200 larvae per group in the treatment groups. 

Was a solvent used? No Solvent type (if applicable): N/A Maximum solvent concentration (if applicable):

N/A EPA recommends that the solvent not exceed 

0.02 ml/L3. OECD recommends that solvent 

have no effect on survival nor produce any 

3 Hutchinson TH, Shillabeer N, Winter MJ, Pickford DB (2006). Acute and chronic effects of carrier solvents in aquatic organisms: A critical review. Review. Aquatic 

Toxicology, 76, pp.69–92. 

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other adverse effects and that concentration 

not be greater than 0.1 ml/L4.

Was a positive control used? Yes Though a positive control was included, no 

mortality, growth, or development data were provided for this treatment. 

Positive control (if applicable): 17  -estrdial [E2] Positive control concentration(s) (if applicable): 

0.0002 mg a.i./L 8 replicate tanks, 2 clusters 

Photoperiod: 12 hrs light : 

12 hrs dark 

EPA recommends photoperiod 12:12 

(light:dark).

Light intensity at water’s surface: 0.1-0.5 Klux EPA recommends light intensity 0.6 – 2 Klux 

(at water’s surface).

Additional details: None provided 

4 OECD (2000). Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. Environmental Health and Safety Publications. Series on 

Testing and Assessment. No. 23. Paris, France. 

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f W n[ a r z } \ llWc h ^ if caW X la_ X ^_ba_ X cW X] ^_o ] _ Xe a

j lm e]n] W_ q a X Wl^cm

e ^o ] o j ooW h ]XejX c

W k ] _a v

f caW X la_ X|V

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Negative Control 0.00 Not Reported. In 

original report, 

mean measured 

were 0.000957, 

0.0234, and 

0.0867 

Not Reported; 

Not reported in 

original report 

In original report range 

of % nominal was 

68-110, 72-116 and 

67-109, respectively 

EPA and OECD recommend that test item 

concentrations be maintained at a coefficient of 

variation (CV)  20%.

Treatment 1 0.001 Treatment 2 0.025 Treatment 3 0.100 

Abbreviations: CV Coefficient of variation. 

Y v wn oac Z W X] ^_o

u] ^ [ ^x ] bW [Y _ d m^ ] _ X o z thyroid histopathology and hind limb length; body weight and snout-vent length were measured in the original study 

 acacW  ] _ d] Z ]d \ W [d W X Wmc^ Z ]d a d Yes

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EPA recommends that observations of mortality and clinical signs occur daily, at a minimum; other observations are recommended as follows: NF 

developmental stage (days 7 and 21); any asynchronous development, indicated by tadpoles that cannot be assigned an NF stage (days 7 and 21); 

hind limb length (days 7 and 21); snout-vent length (days 7 and 21); body weight (test initiation, for optional size-based larval selection); and thyroid 

gland gross pathology and histopathology (day 21). Note the histopathology section of the test guideline also includes thyroid gross pathology observations.

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G G H M L O  N K O J P Q Q G O   O O G S P

 * 
     

No mortality results from the original study were reported. In the original study, the percent mortality at 

test termination, by replicate is summarized below 

        
      	  5     0 2 3 5  / 0 *

Abbreviations: NA Not applicable. 

1 As there were 8 tanks (1-4 and 5-8) per treatment and only one cluster of four was used in this study, the actual 

tank identification for the negative control was Negative control1 tanks 5-8 and Tanks 1-4 for the treatment groups. 

Therefore, replicate A corresponds to either Tank 5 for the NC or tank 1 for the atrazine treatment group, and 

Rep B corresponds to Tank 6 for the NC and Tank 2 for the atrazine groups and so on. 

2

 25/rep for replicate A,C,D. One suspected mishap in Rep B, so % mortality based on 24/rep. 

No results pertaining to NF stage or asynchronous development were reported in this study. In the original 

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study, frogs were exposed till complete metamorophosis NF stage 66 or until day 75 of exposure (83 

days post fertilization). No statistical differences in time to metamorphosis was observed between the 

control and treatment groups. Average time to metamorphosis ranged from 50.9 to 52.9 days. Only 

two frogs in the study that survived until test termination did not complete metamorphosis, with one in 

the 0.1 mg a.i./L group. 

      Ü @  
             	  	  5     0 2 3 5  / 0 Ý          	   & (   	     	   
  	             	 *

Table 10 deleted. 

Normalized hind limb length was calculated by the reviewer and averaged 1.22 in the negative control 

and 1.26, 1.26, and 1.28 in the nominal 0.0010, 0.025, and 0.100 mg a.i./L treatment groups, 

respectively, with increases of 2.9 to 4.6% relative to the negative control. The study authors did not 

analyze normalized hind limb length. 

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Negative Control NA NA NA NA 4 22.7 0.48 1.22 

0.0010 NA NA NA NA 4 23.6 0.59 1.26 

0.025 NA NA NA NA 4 23.2 0.77 1.26 

0.100 NA NA NA NA 4 23.1 0.89 1.28 

Abbreviations: NA Not applicable. SD Standard deviation. 

1

 The normalized value (HLL: SVL) reported in the table should be the treatment mean of individual ratios of hind 

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limb length (HLL) to snout-vent length (SVL). 

2

 Summary results for snout-vent length (SVL) are presented in the next table (      + ). 

The study authors reported that all growth parameters (i.e., HLL, SVL, wet weight) were comparable 

between the three treatment groups and the negative control (p ì 0.071). HLL ranged from 22.7 mm 

in the negative control to 23.6 mm in the 0.0010 mg a.i./L treatment group, with inhibitions ranging -4.1 

to -1.9% relative to the control. Wet weight ranged from 549.2 mg in the negative control to 591.0 mg 

in the 0.0010 mg a.i./L treatment group with increases of 2.2 to 7.6% in all groups relative to the negative 

control. SVL ranged from 18.1 mm in the 0.100 mg a.i./L treatment group to 18.7 mm in the 0.0010 

mg a.i./L treatment group. Inhibitions in SVL were -0.9, 0.5, and 2.5% in the 0.0010, 0.025, and 0.100 

mg a.i./L treatment groups, respectively, relative to the negative control. 

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Negative Control NA NA NA 4 18.5 0.39 NA NA NA 4 549 20 

0.0010 NA NA NA 4 18.7 0.24 NA NA NA 4 591 29 

0.025 NA NA NA 4 18.4 0.56 NA NA NA 4 563 49 

0.100 NA NA NA 4 18.1 0.47 NA NA NA 4 562 53 

Abbreviations: NA Not applicable. ND Not determined. SD Standard deviation. 

1

 Also referred to as “wet weight” in the test guideline.

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No thyroid hypertrophy or atrophy was noted in control or treatment tadpoles. Further, only mild follicular 

cell hypertrophy and hyperplasia were noted, though the occurrence was comparable between treatment 

and control tadpoles. Decreased colloid was also observed, though like thyroid and follicular cell 

observations, was comparable between all groups and did not appear to be treatment-related. In 

evaluating histopathology, frogs were binned according to age at sacrifice (reported as dpf 43-50, dpf 

51-60, and dpf 61-75) and comparisons between treated and control animals were made within each 

age group category. However, in the following tables the observed effects are combined for all binned 

groups. 

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      8 D 
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Negative Control 0 20 20 20 20 20 18 20 20 

1 20 0 20 0 20 2 20 0 

2 20 0 20 0 20 0 20 0 

3 20 0 20 0 20 0 20 0 

0.0010 0 20 20 20 20 20 17 20 19 

1 20 0 20 0 20 3 20 1 

2 20 0 20 0 20 0 20 0 

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0.025 0 20 20 20 20 20 17 20 19 

1 20 0 20 0 20 3 20 1 

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0.100 0 20 20 20 20 20 17 20 20 

1 20 0 20 0 20 3 20 0 

2 20 0 20 0 20 0 20 0 

3 20 0 20 0 20 0 20 0 

1

 Thyroid gland gross pathology and histopathology are graded 0 – 3 based on severity: 0=Not remarkable, 1=Mild, 

2=Moderate, 3=Severe. See OECD No. 82 for reference.

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f W n[ a 

 z jdd]X] ^_W [fe h c^ ]d{[ W_ dú] o X ^mW X

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Potential changes in follicular lumen area, cell height and cell shape were not reported and therefore Table 14 was deleted. 

No clinical signs or sub-lethal effects from the original assay were reported. In the original report, clinical signs of toxicity were reported. The most common 

finding was small size and was observed in the control and atrazine treatment tanks and was never for more than two frogs in a tank. Four frogs were observed with gross abnormalities including one in the NC (not the cluster used here) and one frog in the 0.001 mg a.i./L treatment group was observed 

with reddened color around head and forelimbs. None of the findings reported were considered to be treatment related. 

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Table 15 deleted 

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B.    	 
     
        
      	  
       
  

Differences in hind-limb length were analyzed using a two-sided Jonckheere-Terpstra non-parametric 

trend test in a step-down fashion. Significance was set at  = 0.05 and the FREQ procedure in SAS 

9.2 was used to conduct the tests. Exact p values were obtained using the Monte Carlo option with N 

= 10,000 replications. 

The study authors found no significant treatment-related effects on hind limb length (p = 0.5599), 

snout-vent length (p = 0.9539), or body weight (p = 0.0708). Further, they reported that there were 

no treatment-related patterns in thyroid histopathology observations. 

' *   



  

       
      	  
       
  

&

             

 Replicate values for snout-to-vent length, dry weight, hind-to-limb length, and normalized 

hind-to-limb length were statistically analyzed. Snout-to-vent length, dry weight, and hind-to-limb length data 

did not exhibit a monotonic trend, but the data satisfied the assumptions of normality and homogeneity of 

variances, as determined using Shapiro-Wilk’s and Levene’s tests. The control group was compared to the 

atrazine-treated groups using Dunnett’s test. Data for normalized hind-to-limb length exhibited a suggestive 

monotonically increasing trend, but data did not satisfy parametric assumptions. The control group was 

compared to the treated levels using the Jonckheere-Terpstra test. These analyses were conducted using 

SAS v. 8.1.

'  	       	  

The reviewer’s analysis detected no significant effect on snout-to-vent length, dry weight, or hind-to-limb 

length (p>0.05). There was, however, a slight (3-5%) significant increase in the normalized hind-to-limb 

length values in the treated groups, relative to the negative control (p<0.05; Jonckheere-Terpstra). No 

effects were seen on thyroid histology. All observations were made on frogs at NF stage 66. 

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0.0010 NA NA NA NA NA NA 3 0.029 NA NA NA NA No 0.025 NA NA NA NA NA NA 3 0.044 NA NA NA NA No 0.100 NA NA NA NA NA NA 5 0.010 NA NA NA NA No Statistical test 

NA NA NA 

JonckheereTerpstra NA 

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Abbreviations: Diff. Difference. NA Not applicable.

1 Unless otherwise indicated, effects are reported based on comparison to the clean water control. Conclusions regarding histopathology may be heavily weighted 

by the expert opinion of a board-certified pathologist. 

2 Unless otherwise specified, effects are considered statistically significant at p <0.05. 

3 Hind-limb length is normalized to snout-vent length (SVL). 

4 While not specifically analyzed in this study, in the original study, frogs were exposed until complete metamorphosis (NF stage 66) or until Day 75 of study. 

The original report did analyzed time to metamorphosis and no statistical differences between the control and treatment groups were observed. Discussions about 

asynchronous development were not specifically reported, but abnormal development, or lack of development was and with no mention of any treatment-related effects on development was reported. 

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       D 
  %   	     	 

1,2  	      %    
    	  *

Abbreviations: Diff. Difference. NA Not applicable.

1

 Unless otherwise indicated, effects are reported based on comparison to the negative (clean water) control. 

2

 Unless otherwise specified, effects are considered statistically significant at p < 0.05. 

 * &           	    

This study was not conducted as a traditional AMA study. Rather, preserved specimens from a previous 

study were examined in the context of an AMA study. However, because this was not conducted according 

to OCSPP 890.1100 guidelines, there were several deviations and several pertinent details pertaining 

to experimental design were not included in this report, however, details on culturing, acclimation, and 

study conduct were provided in MRID 47153501. See Section 1. Materials and Methods for details.

C *   



  

    
 



  

The objective of the current study was to evaluate preserved tissues from metamorphosed Xenopus laevis

frogs at NF stage 66 for potential thyroid-dependent effects of atrazine in accordance with guidelines of 

Amphibian Metamorphosis Assay. The frogs in this study were archived specimens that were originally 

utilized for gonad histopathology investigations. The developmental stage of the archived specimens was 

 
     	 

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0.0010 NA NA 1 0.892 NA NA 8 0.359 

0.025 NA NA -0.5 0.981 NA NA 2 0.926 

0.100 NA NA -2 0.338 NA NA 2 0.948 

Statistical test NA Dunnett’s NA Dunnett’s

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beyond that recommended for analysis in the Amphibian Metamorphosis Assay (i.e., < NF stage 60). 

Thyroid-relevant endpoints recommended in the AMA are likely less sensitive by the time metemorphosis 

has completed; however, there were no effects on time to metamorphosis in the original study. 

The reviewer’s conclusions did not agree with the study author’s. The study author concluded that there 

were no significant effects in this study (p>0.05). The reviewer’s analysis detected a significant increases 

of the normalized hind-to-limb length at all treated levels, relative to the control (p<0.05). 

The original study from which preserved specimens were collected and analyzed was conducted at Wildlife 

International Laboratories, Ltd., and included eight replicate tanks per atrazine dose group (0.0010, 

0.025, 0.100 mg a.i./L). The eight tanks were arranged in two clusters of four tanks each and each 

tank contained 25 tadpoles. For the present analysis, a single cluster of four tanks was selected randomly 

from each of the dose groups. The original study also included 16 replicate tanks for the negative control, 

which were arranged in four clusters of four tanks each. One negative control cluster was contaminated 

during the original study and was therefore excluded from the final analysis. Consequently, the single 

cluster of negative control tanks included in this AMA evaluation was selected randomly from among the 

three remaining negative control clusters. However, according to the original report, one cluster of tanks 

was contaminated with atrazine and was excluded from analysis and a microbial bloom (one tank) was 

observed in another cluster of control tanks and was also excluded, thereby leaving two clusters for final 

analysis. As this report indicates that only one cluster (contaminated, reviewer assumed the one with 

atrazine detected) was removed, it means that the other cluster with the microbial bloom was retained 

for potential analysis. However, based on this and the original report, the control animals used in this 

analysis were from a uncompromised control cluster. 

For pathology examination, frogs were binned by age at sacrifice (i.e., 43-50 dpf, 51-60 dpf, and 61-75 

dpf). However, it was not clear if hind limb length measurements were binned by age, but it does not 

appear so, as the data in the appendices of the report do not indicate a binning exercise.. 

Measurements of hind limb lengths were obtained via image analysis from color digital photographs of 

the entire preserved caudal segment. A single image was acquired for each frog, and saved as a 

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high-resolution TIFF file with the animal’s identifying number. A calibration metric rule and identifying 

number were placed within the field of view next to each frog. Due to processing for histology, some hind 

limbs were removed from frogs. For frogs with only one hind limb attached to pelvis, only the attached 

limb was measured. For frogs were both limbs were attached, a coin toss was used to determine if right 

or left leg was measured; it is unclear why the choice of a coin toss was used to determine selection. 

For frogs with no attached limbs, measurements were not taken. 

It was originally intended that hind limb lengths would be measured for each frog that had been selected 

for thyroid histopathology (20 frogs per treatment) plus an additional 40 frogs selected randomly from 

the tank clusters selected for the gonad histopathology evaluation (total hind limb measurements per 

treatment = 60). However, for the control and high dose groups, the numbers of frogs that were suitable 

for analysis (i.e., hind limbs still attached to the pelvic girdle) were <60. Numbers of frogs were 41, 64, 

63, and 35 for the control, low, middle, and high dose treatments, respectively. It is unclear why for 

low and middle concentrations, the number of frogs analyzed is greater than 60. 

In traditional AMA studies, tadpoles 13-15 dpf (NF stage 51) are used and after 21 days, tadpoles are 

34-36 dpf. Tadpoles at NF stages greater than 60 are excluded from analysis of growth parameters due 

to rapid morphological changes in tadpoles at this stage. In the current study, frogs were collected at 

complete metamorphosis, NF 66 and according to the binning exercise in this study appear to have been 

exposed from 43-75 days. Therefore, all frogs should have been at the same stage making comparisons 

in growth appropriate. Time to metamorphosis was not significantly different across the treatment groups. 

The frogs were exposed for 75 days which equated to 83 days post fertilization. As the study reported 

the binning ages as dpf (43-75), it is not sure if this is to indicate days of exposure till metamorphosis 

and not days post fertilization as animals were not exposed until 8 days post fertilization and according 

to raw data, some animals in the treatment groups used in this study were exposed until day 70 (however, 

it is unknown if those specific animals were used in this study). 

G G G H M L ! L M L P  L O

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Grim, KC, Wolfe, M, Braunbeck T, Iguchi T, Ohta Y, Tooi O, Touart L, Wolf DC, Tietge J (2009) Thyroid 

histopathology assessments for the amphibian metamorphosis assay to detect thyroid-active substances. 

Toxicologic Pathology, 37:415-424. 

Hollander M, Wolfe DA (1999) Nonparametric Statistical Methods, Second Edition, New York: John Wiley 

& Sons. 

Hosmer A, Springer T, Kloas W, Lutz I, Wolf J, Holden L (2007) Response of Larval Xenopus laevis to 

Atrazine Exposure: Assessment of Metamorphosis and Gonadal Morphology: Overview and Comparison of 

Studies. Response to Atrazine Generic and Product Specific Data Call-In Notice, March 17, 2004. Syngenta 

Study Number T002536-05. 

Lutz I, Kloas W, Springer TA, Holden LR, Wolf JC, Krueger HO, Hosmer AJ (2008) Development, 

standardization, and refinement of procedures for evaluating effects of endocrine active compounds on 

development and sexual differentiation of Xenopus laevis. Analytical and Bioanalytical Chemistry, 390: 

2031-2048. 

SAS (2009) Base SAS 9.2 Procedures Guide. Cary, NC: SAS Institute, Inc. 

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l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

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l 5 > = _ : 2 2 6 2 F 5 3 2 T

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f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

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Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

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F 5 3 2 i b b b

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l 5 > = _ : 2 2 6 2 F 5 3 2 T

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S i U V B @ 3 1 7 X 2 U 8 2 @ ; 7 > Y Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

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N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

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C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

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l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

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l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

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\ 1 6 ^ b b b

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F 5 3 2 i b b b

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l 5 > = _ : 2 2 6 2 F 5 3 2 T

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S j U V B @ 3 > B ` 2 > 1 ^ 2 > X 1 : U 8 8 Y Y

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N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

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Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

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\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

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l 5 > = _ : 2 2 6 2 F 5 3 2 T

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

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G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

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Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

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l 5 > = _ : 2 2 6 2 F 5 3 2 T

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S n U V B @ : ; > @ B ^ ; 8 < ^ 2 > X 1 : U 8 8 Y Y

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J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

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E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

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b b b b I P F G C G O P M C D E C

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F 5 3 2 i S b b b b b e b

F 5 3 2 j S b b b b b e b

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ b b b b b

F 5 3 2 T b b b b b

F 5 3 2 i b b b b b

F 5 3 2 j b b b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j c g S b V c S b c i V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

F 5 3 2 j b b b

l 5 > = _ : 2 2 6 2 F 5 3 2 T

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j c g S b V c S b c i V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

F 5 3 2 j b b b

l 5 > = _ : 2 2 6 2 F 5 3 2 T

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 EPA MRID Number 48614801

Page = T of  

DER Template Version: 22 September 2011 

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S c U V B @ > 5 6 8 : ; > @ B ^ ; 8 < Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

E : 7 9 ; 6 5 B ] ; ^ _ 3 1 2 3 1 4 5 6 I 5 6 8 7 ^ ; 1 A 5 4 M 2 3 ; @ ? 7 ^ 3 B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S T

J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

b b b b I P F G C G O P M C D E C

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

R G E L \ E N Z Z G M K E C G C L E C L \ E

J 2 ` 2 ^ I Z 2 7 > E 1 @ F 2 ` E 1 @ D 6 6 \ 5 2 4 5 4 Q 7 6 g c h \ 5 > 4 b L > 1 2 6 ` 7 ^

\ 1 6 ^ S b b b b b e b

F 5 3 2 T S b b b b b e b

F 5 3 2 i S b b b b b e b

F 5 3 2 j S b b b b b e b

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ b b b b b

F 5 3 2 T b b b b b

F 5 3 2 i b b b b b

F 5 3 2 j b b b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j c g S b V c S b c i V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

F 5 3 2 j b b b

l 5 > = _ : 2 2 6 2 F 5 3 2 T

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

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 EPA MRID Number 48614801

Page =  of  

DER Template Version: 22 September 2011 

j c g S b V c S b c i V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

F 5 3 2 j b b b

l 5 > = _ : 2 2 6 2 F 5 3 2 T

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S o U i T B @ 3 1 7 X 2 U 8 2 @ ; 7 > Y Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

E : 7 9 ; 6 5 B ] ; ^ _ 3 1 2 3 1 4 5 6 I 5 6 8 7 ^ ; 1 A 5 4 M 2 3 ; @ ? 7 ^ 3 B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S T

J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

b b b b I P F G C G O P M C D E C

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

R G E L \ E N Z Z G M K E C G C L E C L \ E

J 2 ` 2 ^ I Z 2 7 > E 1 @ F 2 ` E 1 @ D 6 6 \ 5 2 4 5 4 Q 7 6 g c h \ 5 > 4 b L > 1 2 6 ` 7 ^

\ 1 6 ^ S b b b b b e b

F 5 3 2 T S b b b b b e b

F 5 3 2 i S b b b b b e b

F 5 3 2 j S b b b b b e b

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ b b b b b

F 5 3 2 T b b b b b

F 5 3 2 i b b b b b

F 5 3 2 j b b b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j c g S b V c S b c i V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

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 EPA MRID Number 48614801

Page = Û of  

DER Template Version: 22 September 2011 

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

F 5 3 2 j b b b

l 5 > = _ : 2 2 6 2 F 5 3 2 T

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j c g S b V c S b c i V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ b b b

F 5 3 2 T b b b

F 5 3 2 i b b b

F 5 3 2 j b b b

l 5 > = _ : 2 2 6 2 F 5 3 2 T

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S V U i T B @ W 2 1 W 2 ; X : 1 U X Y Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

E : 7 9 ; 6 5 B ] ; ^ _ 3 1 2 3 1 4 5 6 I 5 6 8 7 ^ ; 1 A 5 4 M 2 3 ; @ ? 7 ^ 3 B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S T

J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

S b g V j S b p g S T b i V V S b j i o N E D [ G M G Z D C M L \ C D E C E

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

R G E L \ E N Z Z G M K E C G C L E C L \ E

J 2 ` 2 ^ I Z 2 7 > E 1 @ F 2 ` E 1 @ D 6 6 \ 5 2 4 5 4 Q 7 6 g c h \ 5 > 4 b L > 1 2 6 ` 7 ^

\ 1 6 ^ n S b c c S b S i S b S T j b c V S b c i e S b c p

F 5 3 2 T n S b c g S b S j S b S T n b g c S b c n e S b o n

F 5 3 2 i n S b c o S b S c S b S i p b o o S b n g e S b o n

F 5 3 2 j n S b c o S b S c S b S j g b n n S b n p e S b o c

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ S b c c S b c j S b c V b b

F 5 3 2 T S b c p S b c V S b o j T S V b o S B V b o S

F 5 3 2 i S b c o S b c T S b o i T S i b c n B i b c n

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Page =



 of  

DER Template Version: 22 September 2011 

F 5 3 2 j S b c c S b c S S b o j T S i b i j B i b i j

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i S b V V S b c j T

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ S b c c b S b c V b b b b b b

F 5 3 2 T S b c g S b j c g S b c V S b p n S b b b b b

F 5 3 2 i S b c o S b g i o S b c o S b p S j S b V o j b b b b

F 5 3 2 j S b c o S b g n p S b c o S b p S T S b V j S T b S S S b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j j b j S S b j n p

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ S b c c b b

F 5 3 2 T S b c p S b T S j S b g V p

F 5 3 2 i S b c o S b p p g S b V o p

F 5 3 2 j S b c c T b S S S S b c T g

F D \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b J D E E C s G I \ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i S b V V S b c j T

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

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 EPA MRID Number 48614801

Page  Ü of  

DER Template Version: 22 September 2011 

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ B S b c c b B S b c c b b b b b b

F 5 3 2 T B S b c g S b j c g B S b c V S b i o j b b b b b

F 5 3 2 i B S b c o S b g i o B S b c V S b i p T S b V o j b b b b

F 5 3 2 j B S b c o S b g n p B S b c V S b i g T S b V j S T b S S S b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j j b j S S b j n p

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ B S b c c b b

F 5 3 2 T B S b c p S b T S j S b S i i

F 5 3 2 i B S b c o S b p p g S b i j i

F 5 3 2 j B S b c c T b S S S S b n p T

L I \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b m M D G C D M C s G I

\ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S p U i T B @ 3 > B ` 2 > 1 ^ 2 > X 1 : U 8 8 Y Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

E : 7 9 ; 6 5 B ] ; ^ _ 3 1 2 3 1 4 5 6 I 5 6 8 7 ^ ; 1 A 5 4 M 2 3 ; @ ? 7 ^ 3 B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S T

J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

S b g j S S b i n c i b S p T S b T c o N E D [ G M G Z D C M L \ C D E C E

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

R G E L \ E N Z Z G M K E C G C L E C L \ E

J 2 ` 2 ^ I Z 2 7 > E 1 @ F 2 ` E 1 @ D 6 6 \ 5 2 4 5 4 Q 7 6 g c h \ 5 > 4 b L > 1 2 6 ` 7 ^

\ 1 6 ^ n T p b c n S b j g S b i S i b T i T V b g i e T g b T V

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F 5 3 2 T n T p b V i S b i n S b T i T b i g T p b j j e T g b T S

F 5 3 2 i n T p b n c S b c o S b i p j b S T T V b c o e T g b j n

F 5 3 2 j n T p b S p S b n V S b i n i b o i T V b j j e T p b p j

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ T p b n n T p b i S T g b S g b b

F 5 3 2 T T p b o V T p b n p T g b S c T S S b g n B S b g n

F 5 3 2 i T p b c n T V b V g T p b g n g g b c S S b c S

F 5 3 2 j T p b T o T V b c S T p b c S g V b c T i b n g

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i T b c c S b i c i

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ T p b c n b T p b o j b b b b b b

F 5 3 2 T T p b V i S b p g i T p b o j S b o g p b b b b b

F 5 3 2 i T p b n c S b g p T T p b n c S b n p n S b p T V b b b b

F 5 3 2 j T p b S p S b j j p T p b S p S b T S T S b i T i S b o j T b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j j b p o S b i V V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ T p b n n b b

F 5 3 2 T T p b o V S b n g n S b p S V

F 5 3 2 i T p b c n S b o V p S b n n i

F 5 3 2 j T p b T o S b j n o S b S p T

F D \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b J D E E C s G I \ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

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f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i T b c c S b i c i

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ B T p b c n b B T p b n c b b b b b b

F 5 3 2 T B T p b V i S b p g i B T p b n c S b V S V b b b b b

F 5 3 2 i B T p b n c S b g p T B T p b n c S b V n i S b p T V b b b b

F 5 3 2 j B T p b S p S b j j p B T p b n c S b V o S S b i T i S b o j T b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j j b p o S b i V V

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ B T p b n n b b

F 5 3 2 T B T p b o V S b n g n S b T g j

F 5 3 2 i B T p b c n S b o V p S b c c p

F 5 3 2 j B T p b T o S b j n o S b g T g

L I \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b m M D G C D M C s G I

\ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M S g U i T B @ : ; > @ B ^ ; 8 < ^ 2 > X 1 : U 8 8 Y Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

E : 7 9 ; 6 5 B ] ; ^ _ 3 1 2 3 1 4 5 6 I 5 6 8 7 ^ ; 1 A 5 4 M 2 3 ; @ ? 7 ^ 3 B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S T

J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

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C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

S b g V V S b g j j S b n p T S b V S i N E D [ G M G Z D C M L \ C D E C E

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

R G E L \ E N Z Z G M K E C G C L E C L \ E

J 2 ` 2 ^ I Z 2 7 > E 1 @ F 2 ` E 1 @ D 6 6 \ 5 2 4 5 4 Q 7 6 g c h \ 5 > 4 b L > 1 2 6 ` 7 ^

\ 1 6 ^ n i i b o V S b n p S b i n i b T j i T b g S e i j b n j

F 5 3 2 T n i j b c g S b c g S b i g i b n g i i b o c e i n b c i

F 5 3 2 i n i j b T V S b V V S b j g j b j n i T b g n e i n b n S

F 5 3 2 j n i j b T S S b p g S b n c j b p o i T b o p e i n b c i

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ i i b V c i i b S i i j b T c b b

F 5 3 2 T i j b c S i j b S S i n b j o T S n b S V B n b S V

F 5 3 2 i i j b i S i i b i c i n b S n T S i b i n B i b i n

F 5 3 2 j i j b S i i i b T S i n b i c T S T b g T B T b g T

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i T b T o S b j o o

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ i i b o V b i j b T n b b b b b b

F 5 3 2 T i j b c g S b i S i i j b T n S b p g S b b b b b

F 5 3 2 i i j b T V S b o i n i j b T n S b g T j S b p j V b b b b

F 5 3 2 j i j b T S S b V i i i j b T S S b g S g S b V o S S b g g g b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j n b n T S b i i S

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ i i b V c b b

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F 5 3 2 T i j b c S S b T S j S b g V p

F 5 3 2 i i j b i S S b j n o S b p V g

F 5 3 2 j i j b S i S b n g n S b V T i

F D \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b J D E E C s G I \ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i T b T o S b j o o

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ B i i b o V b B i i b o V b b b b b b

F 5 3 2 T B i j b c g S b i S i B i j b i g S b T n S b b b b b

F 5 3 2 i B i j b T V S b o i n B i j b i g S b T n g S b p j V b b b b

F 5 3 2 j B i j b T S S b V i i B i j b i g S b T c n S b V o S S b g g g b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j n b n T S b i i S

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ B i i b V c b b

F 5 3 2 T B i j b c S S b T S j S b S i i

F 5 3 2 i B i j b i S S b j n o S b T i T

F 5 3 2 j B i j b S i S b n g n S b i p p

L I \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b m M D G C D M C s G I

\ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

1 2 3 1 4 5 6 7 8 9 : ; < 8 2 1 7 8 5 6 9 : 3 = 6 2 2 > 3 1 ? @ A B C D E C F G C G G 1 6 7 H ; > 2

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G I G J K E L E M D E N J C E O P M Q G M L G R J D Q G M T S U i T B @ > 5 6 8 : ; > @ B ^ ; 8 < Y

C D E C E P O G E E N Z [ C L P I E O P M [ G M G Z D C M L \ G I G J K E L E

E : 7 9 ; 6 5 B ] ; ^ _ 3 1 2 3 1 4 5 6 I 5 6 8 7 ^ ; 1 A 5 4 M 2 3 ; @ ? 7 ^ 3 B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S T

J 2 ` 2 > 2 3 1 2 3 1 4 5 6 : 5 8 5 X 2 > 2 ; 1 A 5 4 ` 7 6 ; 7 > = 2 U 7 < 3 5 ^ ? 1 2 6 2 3 ; @ ? 7 ^ 3 Y B B 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c

N 3 2 9 7 6 7 8 2 1 6 ; = 7 > 7 ^ A 3 2 3 ; 4 > 2 ; 1 : 2 6 1 2 3 1 6 2 d 2 = 1 2 @ e 5 1 : 2 6 W ; 3 2 > 5 > B 9 7 6 7 8 2 1 6 ; =

7 > 7 ^ A 3 2 3 b

E : 7 9 ; 6 5 B ] ; ^ _ 3 E : 7 9 ; 6 5 B ] ; ^ _ 3 J 2 ` 2 > 2 3 J 2 ` 2 > 2 3 \ 5 > = ^ ? 3 ; 5 >

C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

S b g o S S b o c o j b p c j S b S j p N E D I P I B [ G M G Z D C M L \ C D E C E

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

R G E L \ E N Z Z G M K E C G C L E C L \ E

J 2 ` 2 ^ I Z 2 7 > E 1 @ F 2 ` E 1 @ D 6 6 \ 5 2 4 5 4 Q 7 6 g c h \ 5 > 4 b L > 1 2 6 ` 7 ^

\ 1 6 ^ n T b i i S b S j S b S T i b T c T b T p e T b i o

F 5 3 2 T n T b i o S b S i S b S T T b j c T b i n e T b i g

F 5 3 2 i n T b i o S b S T S b S S S b V o T b i n e T b i V

F 5 3 2 j n T b i p S b S j S b S i i b n V T b i j e T b j j

J 2 ` 2 ^ Z 2 @ ; 7 > Z ; > Z 7 k h 5 4 \ 5 > 1 6 5 ^ U 8 2 7 > 3 Y h M 2 @ ? = 1 ; 5 > U 8 2 7 > 3 Y

\ 1 6 ^ T b i i T b i S T b i c b b

F 5 3 2 T T b i V T b i n T b i p T S j b i V B j b i V

F 5 3 2 i T b i o T b i c T b i V T S i b p o B i b p o

F 5 3 2 j T b i g T b i n T b j T T S n b V S B n b V S

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i n b n p S b S i c

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ T b i i b T b i o b b b b b b

F 5 3 2 T T b i o S b S V T T b i o S b g p V b b b b b

F 5 3 2 i T b i o S b T i T T b i o S b g g i S b g p g b b b b

F 5 3 2 j T b i p S b S T S T b i o S b g g n S b V S n S b c i n b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

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DER Template Version: 22 September 2011 

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j o b V V S b S V g

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X > 2 X 7 1 ; ` 2 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ T b i i b b

F 5 3 2 T T b i V S b T i j S b g V T

F 5 3 2 i T b i o S b S g n S b g c o

F 5 3 2 j T b i g S b T i j S b g g S

F D \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b J D E E C s G I \ P I C M P J

] ; ^ ^ ; 7 8 3 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

l 5 > = _ : 2 2 6 2 t : ; X : 2 3 1 @ 5 3 2 U > 5 3 ; X > b @ ; 4 4 2 6 2 > = 2 3 Y

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

[ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

G > 7 ^ A 3 ; 3 5 4 Q 7 6 ; 7 > = 2 U G I P Q G Y B 5 ` 2 6 7 ^ ^ O B 1 2 3 1

I ? 8 2 6 7 1 5 6 @ 4 F 2 > 5 8 ; > 7 1 5 6 @ 4 O B 3 1 7 1 [ B ` 7 ^ ? 2

j T i n b n p S b S i c

F ? > > 2 1 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 1 : 7 > = 5 > 1 6 5 ^

] ; ^ ^ ; 7 8 3 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

C ? _ 2 A B 1 W 5 B 3 ; @ 2 @ 1 2 3 1 3 e 7 ^ ^ 9 5 3 3 ; < ^ 2 = 5 8 9 7 6 ; 3 5 > 3 e > 5 1 ? 3 2 @ 4 5 6 I P D \ 5 6 J P D \

J 2 ` 2 ^ Z 2 7 > F ? > > 2 1 1 L 3 5 1 5 > ; = ] ; ^ ^ ; 7 8 3 C ? _ 2 A 9 B ` 7 ^ ? 2 3

9 B ` 7 ^ ? 2 8 2 7 > 9 B ` 7 ^ ? 2 F 5 3 2 T F 5 3 2 i F 5 3 2 j F 5 3 2 n F 5 3 2 c

\ 1 6 ^ B T b i i b B T b i i b b b b b b

F 5 3 2 T B T b i o S b S V T B T b i o S b S i i b b b b b

F 5 3 2 i B T b i o S b T i T B T b i o S b S i j S b g p g b b b b

F 5 3 2 j B T b i p S b S T S B T b i p S b S S i S b V S n S b c i n b b b

f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f

f f f

I P I B [ G M G Z D C M L \ G I G J K E D E B ? 3 2 7 ^ 9 : 7 B ^ 2 ` 2 ^ a S b S c 4 5 6 7 ^ ^ 1 2 3 1 3

q 6 ? 3 _ 7 ^ B ] 7 ^ ^ ; 3 1 2 3 1 B 2 r ? 7 ^ ; 1 A 7 8 5 > X 1 6 2 7 1 8 2 > 1 X 6 5 ? 9 3

F 2 X 6 2 2 3 5 4 O 6 2 2 @ 5 8 C 2 3 1 E 1 7 1 [ B ` 7 ^ ? 2

j o b V V S b S V g

Z 7 > > ] : ; 1 B 1 2 3 1 ; > X 2 7 = : 1 6 1 8 2 @ ; 7 > 3 ; X > ; 4 b @ ; 4 4 2 6 2 > 1 4 6 5 8 = 5 > 1 6 5 ^

l 5 > = _ : 2 2 6 2 B 1 2 3 1 7 3 3 ? 8 2 3 @ 5 3 2 B 6 2 3 9 5 > 3 2 6 2 ^ 7 1 ; 5 > 3 : ; 9 e 1 2 3 1 ; > X L I \ M D G E L I m 1 6 2 > @

J 2 ` 2 ^ Z 2 @ ; 7 > Z 7 > > ] : ; 1 9 B ` 7 ^ ? 2 l 5 > = _ : 2 2 6 2 9 B ` 7 ^ ? 2

\ 1 6 ^ B T b i i b b

F 5 3 2 T B T b i V S b T i j S b S i g

F 5 3 2 i B T b i o S b S g n S b S n n

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 EPA MRID Number 48614801

Page   of  

DER Template Version: 22 September 2011 

F 5 3 2 j B T b i g S b T i j S b S T S

L I \ M D G E L I m C M D I F C D E C E N Z Z G M K J P ] D E C \ P I \ D I C M G C L P I E L m I L O b m M D G C D M C s G I

\ P I C M P J

] ; ^ ^ ; 7 8 3 F 5 3 2 T

l 5 > = _ : 2 2 6 2 F 5 3 2 T

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