# Patent

## Title
Maize genomic marker set

## Abstract

Maize markers useful for genotyping and association studies, e.g. association with oil content QTLs in populations derived from the Illinois High Oil and Illinois Low Oil maize lines. Primers and hybridization probes for TaqmanTM assays are provided for 488 SNP markers in 484 loci.

## Background
REFERENCE TO RELATED APPLICATIONS
This application claims priority under 35 U.S.C. 120 to U.S. application Ser. No. 10/806,075, filed Mar. 22, 2004, now U.S. Pat. No. 7,194,205 application Ser. No. 10/613,520, filed Jul. 2, 2003, now abandoned application Ser. No. 10/389,566, filed Mar. 14, 2003 now abandoned which claims priority under 35 U.S.C. 119(e) to U.S. Provisional Application 60/365,301 filed Mar. 15, 2002, 60/391,786 filed Jun. 26, 2002 and 60/392,018 filed Jun. 26, 2002, all of which are incorporated herein by reference in their entireties.
INCORPORATION OF SEQUENCE LISTING
Two copies and a computer readable form of the sequence listing, i.e. labeled “Copy 1 of Sequence Listing”, “Copy 2 of Sequence Listing” and “Computer Readable Form of Sequence Listing”, each on a separate CD-ROM containing the file named “52900H.ST25.txt”, which is 788 kb (measured in MS-Windows) and was created on Jul. 22, 2004, are herein incorporated by reference.
INCORPORATION OF TABLES
Two copies of Table 1, labeled as “Copy 1 of Table 1” and “Copy 2 of Table 1”, each on a separate CD-ROM containing the file named “Table 1.txt”, which is 145 kb (measured in MS-Windows) and was created on Jul. 22, 2004, are herein incorporated by reference. 







TABLES




The patent contains table(s) that have been included at the end of the specification.






FIELD OF THE INVENTION
Disclosed herein are inventions in the field of plant molecular biology, plant genetics and plant breeding. More specifically disclosed are maize genetic markers, i.e. polymorphic DNA, which are useful for discovery and isolation of genes, marker trait association, discovery of QTLs and molecular breeding.
BACKGROUND OF THE INVENTION
Maize, Zea mays L., is one of the major crops grown worldwide as a primary source for animal feed, human food and industrial purposes. Maize plants with improved agronomic traits and maize seed with improved quality traits are desirable for the farmer, processor and consumer of maize and maize derived products. The ability to breed or develop transgenic plants with improved traits depends in part on identification of genes or QTLs associated with a trait. The unique maize sequences disclosed herein are useful as mapping tools to assist in plant breeding, in gene and QTL discovery, as markers in marker trait association and molecular breeding.
Polymorphisms are useful as genetic markers for genotyping applications in the agriculture field, e.g., in plant genetic studies and commercial breeding. See for instance U.S. Pat. Nos. 5,385,835; 5,492,547 and 5,981,832, the disclosures of all of which are incorporated herein by reference. The highly conserved nature of DNA combined with the rare occurrences of stable polymorphisms provide genetic markers that are both predictable and discerning of different genotypes. Among the classes of existing genetic markers are a variety of polymorphisms indicating genetic variation including restriction-fragment-length polymorphisms (RFLPs), amplified fragment-length polymorphisms (AFLPs), simple sequence repeats (SSRs), single nucleotide polymorphisms (SNPs), and insertion/deletion polymorphisms (Indels). Because the number of genetic markers for a plant species is limited, the discovery of additional genetic markers associated with a trait will facilitate genotyping applications including marker-trait association studies, gene mapping, gene discovery, marker-assisted selection, and marker-assisted breeding. Evolving technologies make certain genetic markers more amenable for rapid, large scale use. For instance, technologies for SNP detection indicate that SNPs may be preferred genetic markers.
SUMMARY OF THE INVENTION
This invention provides maize polymorphic markers, more specifically SNP and Indel markers located in 484 polymorphic maize genomic DNA loci having DNA sequence of SEQ ID NO:1 through SEQ ID NO:484. Such markers are useful for discovery and isolation of genes, marker trait association, discovery of QTLs and molecular breeding. This invention also provides primers and probes useful in genotyping with the 488 specific markers within the 484 polymorphic loci. This invention also provides methods of using markers in the 484 polymorphic loci for genotyping maize, e.g. in identifying genes and QTLs, molecular breeding, mapping DNA clones, and the like.


DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS
The following definitions are used to described the markers and their uses.
An “allele” means an alternative sequence at a particular locus; the length of an allele can be as small as 1 nucleotide base but is typically larger. Allelic sequence can be amino acid sequence or nucleic acid sequence.
A “locus” is a short sequence that is usually unique and usually found at one particular location by a point of reference, e.g., a short DNA sequence that is a gene, or part of a gene or intergenic region. A locus of this invention can be a unique PCR product. The loci of this invention are polymorphic between certain individuals.
“Genotype” means the specification of an allelic composition at one or more loci within an individual organism. In the case of diploid organisms, there are two alleles at each locus; a diploid genotype is said to be homozygous when the alleles are the same, and heterozygous when the alleles are different.
“Phenotype” means the detectable characteristics of a cell or organism that are a manifestation of gene expression.
“Marker” means a polymorphic sequence. A “polymorphism” is a variation among individuals in sequence, particularly in DNA sequence. Useful polymorphisms include a single nucleotide polymorphisms (SNPs) and insertions or deletions in DNA sequence (Indels).
“Marker assay” means a method for detecting a polymorphism at a particular locus using a particular method, e.g., phenotype (such as seed color, flower color, or other visually detectable trait), restriction fragment length polymorphism (RFLP), single base extension, electrophoresis, sequence alignment, allelic specific oligonucleotide hybridization (ASO), RAPID, etc. Preferred marker assays include single base extension as disclosed in U.S. Pat. No. 6,013,431 and allelic discrimination where endonuclease activity releases a reporter dye from a hybridization probe as disclosed in U.S. Pat. No. 5,538,848, the disclosures of both of which are incorporated herein by reference.
“Linkage” refers to relative frequency at which types of gametes are produced in a cross. For example, if locus A has alleles “A” or “a” and locus B has alleles “B” or “b,” a cross between parent I with AABB and parent II with aabb will produce four possible gametes where the haploid genotypes are segregated into AB, Ab, aB and ab. The null expectation is that there will be independent and equal segregation into each of the four possible genotypes, i.e., with no linkage, 1⁄4 of the gametes will be of each genotype. Segregation of gametes into a genotypes differing from 1⁄4 are attributed to linkage. Two loci are said to be “genetically linked” when they show this deviation from the expected equal frequency of 1⁄4.
“Linkage disequilibrium” is defined in the context of the relative frequency of gamete types in a population of many individuals in a single generation. If the frequency of allele A is p, a is p′, B is q and b is q′, then the expected frequency (with no linkage disequilibrium) of genotype AB is pq, Ab is pq′, aB is p′q and ab is p′q′. Any deviation from the expected frequency is called linkage disequilibrium.
“Quantitative Trait Locus (QTL)” means a locus that controls to some degree numerically representable traits that are usually continuously distributed.
“Haplotype” means the genotype for multiple loci or genetic markers in a haploid gamete. Generally these loci or markers reside within a relatively small and defined region of a chromosome. A preferred haplotype comprises the 10 cM region or the 5 cM region or the 2 cM region surrounding an informative marker having a significant association with oil.
“Hybridizing” means the capacity of two nucleic acid molecules or fragments thereof to form anti-parallel, double-stranded nucleotide structure. The nucleic acid molecules of this invention are capable of hybridizing to other nucleic acid molecules under certain circumstances. A nucleic acid molecule is said to be the “complement” of another nucleic acid molecule if the molecules exhibit “complete complementarity,” i.e., each nucleotide in one sequence is complementary to its base pairing partner nucleotide in another sequence. Two molecules are said to be “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional “low-stringency” conditions. Similarly, the molecules are said to be “complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional “high-stringency” conditions. Nucleic acid molecules that hybridize to other nucleic acid molecules, e.g., at least under low stringency conditions are said to be “hybridizable cognates” of the other nucleic acid molecules. Conventional stringency conditions are described by Sambrook et al., Molecular Cloning, A Laboratory Manual, 2nd Ed., Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1989) and by Haymes et al., Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, D.C. (1985), each of which is incorporated herein by reference. Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure. Thus, in order for a nucleic acid molecule to serve as a primer or probe, it need only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations employed. Appropriate stringency conditions that promote DNA hybridization, for example, 6.0× sodium chloride/sodium citrate (SSC) at about 45° C., followed by a wash of 2.0×SSC at 50° C., are known to those skilled in the art or can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6, incorporated herein by reference. For example, the salt concentration in the wash step can be selected from a low stringency of about 2.0×SSC at 50° C. to a high stringency of about 0.2×SSC at 50° C. In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature, about 22° C., to high stringency conditions at about 65° C. Both temperature and salt may be varied, or either the temperature or the salt concentration may be held constant while the other variable is changed.
“Sequence identity” refers to the extent to which two optimally aligned polynucleotide or peptide sequences are invariant throughout a window of alignment of components, e.g., nucleotides or amino acids. An “identity fraction” for aligned segments of a test sequence and a reference sequence is the number of identical components that are shared by the two aligned sequences divided by the total number of components in reference sequence segment, i.e., the entire reference sequence or a smaller defined part of the reference sequence. “Percent identity” is the identity fraction times 100. Optimal alignment of sequences for aligning a comparison window are well known to those skilled in the art and may be conducted by tools such as the local homology algorithm of Smith and Waterman, the homology alignment algorithm of Needleman and Wunsch, the search for similarity method of Pearson and Lipman, and preferably by computerized implementations of these algorithms such as GAP, BESTFIT, FASTA, and TFASTA available as part of the GCG® Wisconsin Package® (Accelrys Inc. Burlington, Mass.). Polynucleotides of the present invention that are variants of the polynucleotides provided herein will generally demonstrate significant identity with the polynucleotides provided herein. Of particular interest are polynucleotide homologs having at least about 70% sequence identity, at least about 80% sequence identity, at least about 90% sequence identity, and more preferably even greater, such as 98% or 99% sequence identity with polynucleotide sequences described herein.
“Purified” refers to a nucleic acid molecule or polypeptide separated from substantially all other molecules normally associated with it in its native state. More preferably, a substantially purified molecule is the predominant species present in a preparation. A substantially purified molecule may be greater than 60% free or 75% free or 90% free or 95% free from the other molecules (exclusive of solvent) present in the natural mixture. The terms “isolated and purified” and “substantially purified” are not intended to encompass molecules present in their native state.
Characteristics of Maize Markers
The maize loci of this invention comprise a DNA sequence that comprises at least 20 consecutive nucleotides and includes or is adjacent to one or more polymorphisms identified in Table 1. Such maize loci have a nucleic acid sequence having at least 90% sequence identity or at least 95% or for some alleles at least 98% and in many cases at least 99% sequence identity, to the sequence of the same number of nucleotides in either strand of a segment of maize DNA that includes or is adjacent to the polymorphism. The nucleotide sequence of one strand of such a segment of maize DNA may be found in a polymorphic locus with a sequence in the group consisting of SEQ ID NO:1 through SEQ ID NO:186. It is understood by the very nature of polymorphisms that for at least some alleles there will be no identity to the polymorphism, per se. Thus, sequence identity can be determined for sequence that is exclusive of the polymorphism sequence. The polymorphisms in each locus are identified more particularly in Table 1.
For many genotyping applications it is useful to employ as markers polymorphisms from more than one locus. Thus, aspects of the invention use a collection of different loci. The number of loci in such a collection can vary but will be a finite number, e.g., as few as 2 or 5 or 10 or 25 loci or more, for instance up to 40 or 75 or 100 or more loci.
Another aspect of the invention provides nucleic acid molecules that are capable of hybridizing to the polymorphic maize loci of this invention, e.g. PCR primers and hybridization probes. In certain embodiments of the invention, e.g., which provide PCR primers, such molecules comprise at least 15 nucleotide bases. Molecules useful as primers can hybridize under high stringency conditions to one of the strands of a segment of DNA in a polymorphic locus of this invention. Primers for amplifying DNA are provided in pairs, i.e., a forward primer and a reverse primer. One primer will be complementary to one strand of DNA in the locus and the other primer will be complementary to the other strand of DNA in the locus, i.e., the sequence of a primer is at least 90% or at least 95% identical to a sequence of the same number of nucleotides in one of the strands. It is understood that such primers can hybridize to a sequence in the locus that is distant from the polymorphism, e.g., at least 5, 10, 20, 50 or up to about 100 nucleotide bases away from the polymorphism. Design of a primer of this invention will depend on factors well known in the art, e.g., avoidance of repetitive sequence.
Another aspect of the nucleic acid molecules of this invention are hybridization probes for polymorphism assays. In one aspect of the invention such probes are oligonucleotides comprising at least 12 nucleotide bases and a detectable label. The purpose of such a molecule is to hybridize, e.g., under high stringency conditions, to one strand of DNA in a segment of nucleotide bases that includes or is adjacent to the polymorphism of interest in an amplified part of a polymorphic locus. Such oligonucleotides are at least 90% or at least 95% identical to the sequence of a segment of the same number of nucleotides in one strand of maize DNA in a polymorphic locus. The detectable label can be a radioactive element or a dye. In preferred aspects of the invention, the hybridization probe further comprises a fluorescent label and a quencher, e.g., for use in hybridization probe assays of the type known as Taqman assays, available from Applied Biosystems of Foster City, Calif.
For assays where the molecule is designed to hybridize adjacent to a polymorphism that is detected by single base extension, e.g., of a labeled dideoxynucleotide, such molecules can comprise at least 15 or at least 16 or 17 nucleotide bases in a sequence that is at least 90% or at least 95% identical to a sequence of the same number of consecutive nucleotides in either strand of a segment of polymorphic maize DNA. Oligonucleotides for single base extension assays are available from Orchid Bioystems.
Such primer and probe molecules are generally provided in groups of two primers and one or more probes for use in genotyping assays. Moreover, it is often desirable to conduct a plurality of genotyping assays for a plurality of polymorphisms. Thus, this invention also provides collections of nucleic acid molecules, e.g., in sets that characterize a plurality of polymorphisms.
Detecting Polymorphisms
Polymorphisms in DNA sequences can be detected by a variety of effective methods well known in the art including those methods disclosed in U.S. Pat. Nos. 5,468,613 and 5,217,863 by hybridization to allele-specific oligonucleotides; in U.S. Pat. Nos. 5,468,613 and 5,800,944 by probe ligation; in U.S. Pat. No. 5,616,464 by probe linking; and in U.S. Pat. Nos. 6,004,744; 6,013,431; 5,595,890; 5,762,876; and 5,945,283 by labeled base extension, all of which are incorporated herein by reference.
In another preferred method for detecting polymorphisms, SNPs and Indels can be detected by methods disclosed in U.S. Pat. Nos. 5,210,015; 5,876,930; and 6,030,787 in which an oligonucleotide probe having a 5′fluorescent reporter dye and a 3′quencher dye covalently linked to the 5′ and 3′ ends of the probe. When the probe is intact, the proximity of the reporter dye to the quencher dye results in the suppression of the reporter fluorescence, e.g., by Forster-type energy transfer. During PCR forward and reverse primers hybridize to a specific sequence of the target DNA flanking a polymorphism. The hybridization probe hybridizes to polymorphism-containing sequence within the amplified PCR product. In the subsequent PCR cycle DNA polymerase with 5′→3′ exonuclease activity cleaves the probe and separates the reporter dye from the quencher dye resulting in increased fluorescence of the reporter. A useful assay is available from AB Biosystems as the Taqman® assay, which employs four synthetic oligonucleotides in a single reaction that concurrently amplifies the maize genomic DNA, discriminates between the alleles present, and directly provides a signal for discrimination and detection. Two of the four oligonucleotides serve as PCR primers and generate a PCR product encompassing the polymorphism to be detected. Two others are allele-specific fluorescence-resonance-energy-transfer (FRET) probes. FRET probes incorporate a fluorophore and a quencher molecule in close proximity so that the fluorescence of the fluorophore is quenched. The signal from a FRET probe is generated by degradation of the FRET oligonucleotide, so that the fluorophore is released from proximity to the quencher, and is thus able to emit light when excited at an appropriate wavelength. In the assay, two FRET probes bearing different fluorescent reporter dyes are used, where a unique dye is incorporated into an oligonucleotide that can anneal with high specificity to only one of the two alleles. Useful reporter dyes include 6-carboxy-4,7,2′,7′-tetrachlorofluorecein (TET), VIC (a dye from Applied Biosystems Foster City, Calif.), and 6-carboxyfluorescein phosphoramidite (FAM). A useful quencher is 6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA). Additionally, the 3′end of each FRET probe is chemically blocked so that it cannot act as a PCR primer. During the assay, maize genomic DNA is added to a buffer containing the two PCR primers and two FRET probes. Also present is a third fluorophore used as a passive reference, e.g., rhodamine X (ROX), to aid in later normalization of the relevant fluorescence values (correcting for volumetric errors in reaction assembly). Amplification of the genomic DNA is initiated. During each cycle of the PCR, the FRET probes anneal in an allele-specific manner to the template DNA molecules. Annealed (but not non-annealed) FRET probes are degraded by TAQ DNA polymerase as the enzyme encounters the 5′ end of the annealed probe, thus releasing the fluorophore from proximity to its quencher. Following the PCR reaction, the fluorescence of each of the two fluorescers, as well as that of the passive reference, is determined fluorometrically. The normalized intensity of fluorescence for each of the two dyes will be proportional to the amounts of each allele initially present in the sample, and thus the genotype of the sample can be inferred.
To design primers and probes for the assay the locus sequence is first masked to prevent design of any of the three primers to sites that match known maize repetitive elements (e.g., transposons) or are of very low sequence complexity (di- or tri-nucleotide repeat sequences). Design of primers to such repetitive elements will result in assays of low specificity, through amplification of multiple loci or annealing of the FRET probes to multiple sites.
PCR primers are designed (a) to have a length in the size range of 18 to 25 bases and matching sequences in the polymorphic locus, (b) to have a calculated melting temperature in the range of 57° C. to 60° C., e.g., corresponding to an optimal PCR annealing temperature of 52° C. to 55° C., (c) to produce a product that includes the polymorphic site and has a length in the size range of 75 to 250 base pairs. The PCR primers are preferably located on the locus so that the polymorphic site is at least one base away from the 3′ end of each PCR primer. The PCR primers must not contain regions that are extensively self- or inter-complementary.
FRET probes are designed to span the sequence of the polymorphic site, preferably with the polymorphism located in the 3′ most 2⁄3 of the oligonucleotide. In the preferred embodiment, the FRET probes will have incorporated at their 3′end a chemical moiety that, when the probe is annealed to the template DNA, binds to the minor groove of the DNA, thus enhancing the stability of the probe-template complex. The probes should have a length in the range of 12 to 17 bases and, with the 3′MGB, have a calculated melting temperature of 5° C. to 7° C. above that of the PCR primers. Probe design is disclosed in U.S. Pat. Nos. 5,538,848; 6,084,102; and 6,127,121.
Use of Polymorphisms to Establish Marker/Trait Associations
The polymorphisms in the loci of this invention can be used in marker/trait associations that are inferred from statistical analysis of genotypes and phenotypes of the members of a population. These members may be individual organisms, e.g., maize, families of closely related individuals, inbred lines, dihaploids or other groups of closely related individuals. Such maize groups are referred to as “lines”, indicating line of descent. The population may be descended from a single cross between two individuals or two lines (e.g., a mapping population) or it may consist of individuals with many lines of descent. Each individual or line is characterized by a single or average trait phenotype and by the genotypes at one or more marker loci.
Several types of statistical analysis can be used to infer marker/trait association from the phenotype/genotype data, but a basic idea is to detect markers, i.e., polymorphisms, for which alternative genotypes have significantly different average phenotypes. For example, if a given marker locus A has three alternative genotypes (AA, Aa and aa), and if those three classes of individuals have significantly different phenotypes, then one infers that locus A is associated with the trait. The significance of differences in phenotype may be tested by several types of standard statistical tests such as linear regression of marker genotypes on phenotype or analysis of variance (ANOVA). Commercially available, statistical software packages commonly used to do this type of analysis include SAS Enterprise Miner (SAS Institute Inc., Cary, N.C.) and Splus (Insightful Corporation. Cambridge, Mass.).
Often the goal of an association study is not simply to detect marker/trait associations, but to estimate the location of genes affecting the trait directly (i.e., QTLs) relative to the marker locations. In a simple approach to this goal, one makes a comparison among marker loci of the magnitude of difference among alternative genotypes or the level of significance of that difference. Trait genes are inferred to be located nearest the marker(s) that have the greatest associated genotypic difference. In a more complex analysis, such as interval mapping (Lander and Botstein, Genetics 121:185-199, 1989), each of many positions along the genetic map (say at 1 cM intervals) is tested for the likelihood that a QTL is located at that position. The genotype/phenotype data are used to calculate for each test position a LOD score (log of likelihood ratio). When the LOD score exceeds a critical threshold value, there is significant evidence for the location of a QTL at that position on the genetic map (which will fall between two particular marker loci).
1. Linkage Disequilibrium Mapping and Association Studies
Another approach to determining trait gene location is to analyze trait-marker associations in a population within which individuals differ at both trait and marker loci. Certain marker alleles may be associated with certain trait locus alleles in this population due to population genetic process such as the unique origin of mutations, founder events, random drift and population structure. This association is referred to as linkage disequilibrium. In linkage disequilibrium mapping, one compares the trait values of individuals with different genotypes at a marker locus. Typically, a significant trait difference indicates close proximity between marker locus and one or more trait loci. If the marker density is appropriately high and the linkage disequilibrium occurs only between very closely linked sites on a chromosome, the location of trait loci can be very precise.
A specific type of linkage disequilibrium mapping is known as association studies. This approach makes use of markers within candidate genes, which are genes that are thought to be functionally involved in development of the trait because of information such as biochemistry, physiology, transcriptional profiling and reverse genetic experiments in model organisms. In association studies, markers within candidate genes are tested for association with trait variation. If linkage disequilibrium in the study population is restricted to very closely linked sites (i.e., within a gene or between adjacent genes), a positive association provides nearly conclusive evidence that the candidate gene is a trait gene.
2. Positional Cloning and Transgenic Applications
Traditional linkage mapping typically localizes a trait gene to an interval between two genetic markers (referred to as flanking markers). When this interval is relatively small (say less than 1 Mb), it becomes feasible to precisely identify the trait gene by a positional cloning procedure. A high marker density is required to narrow down the interval length sufficiently. This procedure requires a library of large insert genomic clones (such as a BAC library), where the inserts are pieces (usually 100-150 kb in length) of genomic DNA from the species of interest. The library is screened by probe hybridization or PCR to identify clones that contain the flanking marker sequences. Then a series of partially overlapping clones that connects the two flanking clones (a “contig”) is built up through physical mapping procedures. These procedures include fingerprinting, STS content mapping and sequence-tagged connector methodologies. Once the physical contig is constructed and sequenced, the sequence is searched for all transcriptional units. The transcriptional unit that corresponds to the trait gene can be determined by comparing sequences between mutant and wild type strains, by additional fine-scale genetic mapping, and/or by functional testing through plant transformation. Trait genes identified in this way become leads for transgenic product development. Similarly, trait genes identified by association studies with candidate genes become leads for transgenic product development.
3. Marker-Aided Breeding and Marker-Assisted Selection
When a trait gene has been localized in the vicinity of genetic markers, those markers can be used to select for improved values of the trait without the need for phenotypic analysis at each cycle of selection. In marker-aided breeding and marker-assisted selection, associations between trait genes and markers are established initially through genetic mapping analysis (as in M.1 or M.2). In the same process, one determines which marker alleles are linked to favorable trait gene alleles. Subsequently, marker alleles associated with favorable trait gene alleles are selected in the population. This procedure will improve the value of the trait provided that there is sufficiently close linkage between markers and trait genes. The degree of linkage required depends upon the number of generations of selection because, at each generation, there is opportunity for breakdown of the association through recombination.
4. Prediction of Crosses for New Inbred Line Development
The associations between specific marker alleles and favorable trait gene alleles also can be used to predict what types of progeny may segregate from a given cross. This prediction may allow selection of appropriate parents to generation populations from which new combinations of favorable trait gene alleles are assembled to produce a new inbred line. For example, if line A has marker alleles previously known to be associated with favorable trait alleles at loci 1, 20 and 31, while line B has marker alleles associated with favorable effects at loci 15, 27 and 29, then a new line could be developed by crossing A×B and selecting progeny that have favorable alleles at all 6 trait loci.
5. Hybrid Prediction
Commercial corn seed is produced by making hybrids between two elite inbred lines that belong to different “heterotic groups”. These groups are sufficiently distinct genetically that hybrids between them show high levels of heterosis or hybrid vigor (i.e., increased performance relative to the parental lines). By analyzing the marker constitution of good hybrids, one can identify sets of alleles at different loci in both male and female lines that combine well to produce heterosis. Understanding these patterns, and knowing the marker constitution of different inbred lines, can allow prediction of the level of heterosis between different pairs of lines. These predictions can narrow down the possibilities of which line(s) of opposite heterotic group should be used to test the performance of a new inbred line.
6. Identity by Descent
One theory of heterosis predicts that regions of identity by descent (IBD) between the male and female lines used to produce a hybrid will reduce hybrid performance. Identity by descent can be inferred from patterns of marker alleles in different lines. An identical string of markers at a series of adjacent loci may be considered identical by descent if it is unlikely to occur independently by chance. Analysis of marker fingerprints in male and female lines can identify regions of IBD. Knowledge of these regions can inform the choice of hybrid parents, because avoiding IBD in hybrids is likely to improve performance. This knowledge may also inform breeding programs in that crosses could be designed to produce pairs of inbred lines (one male and one female) that show little or no IBD.
A fingerprint of an inbred line is the combination of alleles at a set of marker loci. High density fingerprints can be used to establish and trace the identity of germplasm, which has utility in germplasm ownership protection.
Genetic markers are used to accelerate introgression of transgenes into new genetic backgrounds (i.e., into a diverse range of germplasm). Simple introgression involves crossing a transgenic line to an elite inbred line and then backcrossing the hybrid repeatedly to the elite (recurrent) parent, while selecting for maintenance of the transgene. Over multiple backcross generations, the genetic background of the original transgenic line is replaced gradually by the genetic background of the elite inbred through recombination and segregation. This process can be accelerated by selection on marker alleles that derive from the recurrent parent.
Use of Polymorphism Assay for Mapping a Library of DNA Clones
The polymorphisms and loci of this invention are useful for identifying and mapping DNA sequence of QTLs and genes linked to the polymorphisms. For instance, BAC or YAC clone libraries can be queried using polymorphisms linked to a trait to find a clone containing specific QTLs and genes associated with the trait. For instance, QTLs and genes in a plurality, e.g., hundreds or thousands, of large, multi-gene sequences can be identified by hybridization with an oligonucleotide probe that hybridizes to a mapped and/or linked polymorphism. Such hybridization screening can be improved by providing clone sequence in a high density array. The screening method is more preferably enhanced by employing a pooling strategy to significantly reduce the number of hybridizations required to identify a clone containing the polymorphism. When the polymorphisms are mapped, the screening effectively maps the clones.
For instance, in a case where thousands of clones are arranged in a defined array, e.g., in 96-well plates, the plates can be arbitrarily arranged in three-dimensionally, arrayed stacks of wells each comprising a unique DNA clone. The wells in each stack can be represented as discrete elements in a three dimensional array of rows, columns and plates. In one aspect of the invention the number of stacks and plates in a stack are about equal to minimize the number of assays. The stacks of plates allow the construction of pools of cloned DNA.
For a three-dimensionally arrayed stack, pools of cloned DNA can be created for (a) all of the elements in each row, (b) all of the elements of each column, and (c) all of the elements of each plate. Hybridization screening of the pools with an oligonucleotide probe that hybridizes to a polymorphism unique to one of the clones will provide a positive indication for one column pool, one row pool and one plate pool, thereby indicating the well element containing the target clone.
In the case of multiple stacks, additional pools of all of the clone DNA in each stack allows indication of the stack having the row-column-plate coordinates of the target clone. For instance, a 4608 clone set can be disposed in 48 96-well plates. The 48 plates can be arranged in 8 sets of 6-plate stacks providing 6×12×8 three-dimensional arrays of elements, i.e., each stack comprises 6 stacks of 8 rows and 12 columns. For the entire clone set there are 36 pools, i.e., 6 stack pools, 8 row pools, 12 column pools and 8 stack pools. Thus, a maximum of 36 hybridization reactions is required to find the clone harboring QTLs or genes associated or linked to each mapped polymorphism.
Once a clone is identified, genes within that clone can be tested for whether they affect the trait by analysis of recombinants in a mapping population, further linkage disequilibrium analysis, and ultimately transgenic testing. Additional genes can be identified by finding additional clones overlapping the one containing the original polymorphism through contig building, as described above.
Breeding Plants of the Invention
In addition to direct transformation of a particular plant genotype with a construct prepared according to the current invention, transgenic plants may be made by crossing a plant having a construct of the invention to a second plant lacking the construct. For example, a selected coding region operably linked to a promoter can be introduced into a particular plant variety by crossing, without the need for ever directly transforming a plant of that given variety. Therefore, the current invention not only encompasses a plant directly regenerated from cells that have been transformed in accordance with the current invention, but also the progeny of such plants. As used herein the term “progeny” denotes the offspring of any generation of a parent plant prepared in accordance with the instant invention, wherein the progeny comprises a construct prepared in accordance with the invention. “Crossing” a plant to provide a plant line having one or more added transgenes relative to a starting plant line, as disclosed herein, is defined as the techniques that result in a transgene of the invention being introduced into a plant line by crossing a starting line with a donor plant line that comprises a transgene of the invention. To achieve this one could, for example, perform the following steps:

    
    
        (a) plant seeds of the first (starting line) and second (donor plant line that comprises a transgene of the invention) parent plants;
        (b) grow the seeds of the first and second parent plants into plants that bear flowers;
        (c) pollinate a flower from the first parent plant with pollen from the second parent plant; and
        (d) harvest seeds produced on the parent plant bearing the fertilized flower.

Backcrossing is herein defined as the process including the steps of:

        (a) crossing a plant of a first genotype containing a desired gene, DNA sequence or element to a plant of a second genotype lacking the desired gene, DNA sequence or element;
        (b) selecting one or more progeny plants containing the desired gene, DNA sequence or element;
        (c) crossing the progeny plant to a plant of the second genotype; and
        (d) repeating steps (b) and (c) for the purpose of transferring the desired gene, DNA sequence or element from a plant of a first genotype to a plant of a second genotype.

Plant Breeding

    
    


Introgression of a DNA element into a plant genotype is defined as the result of the process of backcross conversion. A plant genotype into which a DNA sequence has been introgressed may be referred to as a backcross converted genotype, line, inbred, or hybrid. Similarly a plant genotype lacking the desired DNA sequence may be referred to as an unconverted genotype, line, inbred, or hybrid.
Backcrossing can be used to improve a starting plant. Backcrossing transfers a specific desirable trait from one source to an inbred or other plant that lacks that trait. This can be accomplished, for example, by first crossing a superior inbred (A) (recurrent parent) to a donor inbred (non-recurrent parent), which carries the appropriate gene(s) for the trait in question, for example, a construct prepared in accordance with the current invention. The progeny of this cross first are selected in the resultant progeny for the desired trait to be transferred from the non-recurrent parent, then the selected progeny are mated back to the superior recurrent parent (A). After five or more backcross generations with selection for the desired trait, the progeny are hemizygous for loci controlling the characteristic being transferred but are like the superior parent for most or almost all other genes. The last backcross generation would be selfed to give progeny that are pure breeding for the gene(s) being transferred, i.e., one or more transformation events.
Therefore, through a series a breeding manipulations, a selected transgene may be moved from one line into an entirely different line without the need for further recombinant manipulation. Transgenes are valuable in that they typically behave genetically as any other gene and can be manipulated by breeding techniques in a manner identical to any other corn gene. Therefore, one may produce inbred plants that are true breeding for one or more transgenes. By crossing different inbred plants, one may produce a large number of different hybrids with different combinations of transgenes. In this way, plants may be produced that have the desirable agronomic properties frequently associated with hybrids (“hybrid vigor”), as well as the desirable characteristics imparted by one or more transgene(s).
It is desirable to introgress the genes of the present invention into maize hybrids for characterization of the phenotype conferred by each gene in a transformed plant. The host genotype into which the transgene was introduced, preferably LH59, is an elite inbred and therefore only limited breeding is necessary in order to produce high yielding maize hybrids. The transformed plant, regenerated from callus is crossed, to the same genotype, e.g., LH59. The progeny are self-pollinated twice, and plants homozygous for the transgene are identified. Homozygous transgenic plants are crossed to a testcross parent in order to produce hybrids. The test cross parent is an inbred belonging to a heterotic group that is different from that of the transgenic parent and for which it is known that high yielding hybrids can be generated, for example hybrids are produced from crosses of LH59 to either LH195 or LH200.
The following examples illustrate the identification of polymorphic markers useful for mapping and isolating genes of this invention and as markers of QTLs and genes associated with an oil-related trait. Other examples illustrate the identification of oil-related genes and partial genes. Still other examples illustrate methods for inserting genes of this invention into a plant expression vector, i.e., operably linked to a promoter and other regulatory elements, to confer an oil-related trait to a transgenic plant.
Example 1
This example illustrates the identification of polymorphic maize markers of this invention.
A set of more than 800 candidate oil genes was identified (a) as homologs of plant genes that are believed to be in an oil-related metabolic pathway of a model plant such as Arabidopsis thaliana; (b) by comparing transcription profiling results for high oil and low oil maize lines; and (c) by subtractive hybridization between endosperm tissues of high oil and low oil lines. The sequences of the candidate oil genes were queried against a proprietary collection of maize genes and partial maize genes, e.g., genomic sequence or ESTs, to identify a set of DNA sequences for candidate maize markers.
Maize polymorphisms were identified by comparing alignments of DNA sequences from separate maize lines. Candidate polymorphisms were qualified by the following parameters:

    
    
        The minimum length of sequence for a synthetic reference sequence is 200 bases.
        The percentage identity of observed bases in a region of 15 bases on each side of a candidate SNP, is 75%.
        The minimum BLAST quality in each of the various sequences at a polymorphism site is 35.
        The minimum BLAST quality in a region of 15 bases on each side of the polymorphism site is 20.
    
    


The SNP and Indel polymorphisms in each locus were qualified for detection by development of an assay, e.g., Taqman® assay (Applied Biosystems, Foster City, Calif.). Assay qualified polymorphisms are evaluated for oil informativeness by comparing allelic frequencies in the two parental lines of an association study population. The parent lines were an oil rich maize line and an oil poor maize line, i.e., the University of Illinois High Oil and Low Oil maize lines as described by Dudley and Lambert (1992, Maydica 37: 81-87). Informativeness is reported as an allelic frequency difference between parental populations, i.e. the high oil line and the low oil line. When one of the parents, e.g., the high oil line, is fixed, its allelic frequency is 1. Markers were qualified if they had an allelic frequency difference of at least 0.6. If the marker was fixed on either parent with a frequency of 0 or 1, a marker could be selected at a lower allelic frequency difference of at least 0.4. The informative markers were viewed on a genetic map to identify marker-deficient regions of chromosomes. Markers with lower allelic frequency difference, e.g., as low as 0.15, were selected to fill in the marker-deficient regions of chromosomes.
DNA amplicons of 484 polymorphic maize genomic DNA loci of this invention are provided in the sequence listing as SEQ ID NO:1 through SEQ ID NO:484. Table 1 provides a description of polymorphisms in the 484 DNA amplicons. Particular aspects of the markers are identified in Table 1 by reference to:
SEQ_NUM, which refers to the sequence number of a nucleic acid sequence in the sequence listing, e.g. SEQ ID NO:1; and
SEQ_ID, which refers to an arbitrary identifying name for an amplicon of a polymorphic locus, e.g. “Amplicon25”;
MUTATION_ID, which refers to one or more arbitrary identifying names for each polymorphism, e.g. “91”;
START_POS which refers to the position in the nucleotide sequence of the polymorphic maize DNA locus where the polymorphism begins, e.g. 110;
END_POS which refers to the position in the nucleotide sequence of the polymorphic maize DNA locus where the polymorphism ends; for SNPs the START_POS and END_POS are common;
TYPE which refers to the identification of the polymorphism as an SNP or IND (Indel);
ALLELEn and STRAINn which refer to the nucleotide sequence of a polymorphism in a specific allelic maize variety, e.g. “C”, “T”, and when strains are indicated “mo17” and “b73”;
Taqman® assays for 488 markers in the 484 polymorphic loci (4 loci are represented by marker assays for 2 separate polymorphisms) are characterized by four separate DNA molecules, i.e. a forward PCR primer, a reverse PCR primer, a VIC-labeled hybridization probe and a FAM-labeled hybridization probe, identified by SEQ ID NO:485 through SEQ ID NO:2436. The primers and probes for each of the 488 markers are more particularly identified in Table 2 by reference to:
Chromosome which refers to one of ten maize chromosomes.
Position which refers to distance to the marker measured in cM from the 5′ end of the chromosome.
Marker which corresponds to the MUTATION_ID in the amplicon of SEQ_NUM (1-484).
Forward designates the number in the Sequence Listing for the DNA sequence of the forward primer.
Reverse designates the number in the Sequence Listing for the DNA sequence of the reverse primer.
FAM designates the number in the Sequence Listing for the FAM-labeled hybridization probe.
VIC designates the number in the Sequence Listing for the VIC-labeled hybridization probe.














TABLE 2





Chromosome
Position
Marker
SEQ_NUM
Forward
Reverse
FAM
VIC






















1
3.7
111829
406
2121
2122
2123
2124


1
5.6
147181
442
2265
2266
2267
2268


1
14.9
36199
222
1381
1382
1383
1384


1
17.3
25418
149
1081
1082
1083
1084


1
22.4
28164
155
1105
1106
1107
1108


1
25.1
43230
269
1573
1574
1575
1576


1
30.4
2847
11
525
526
527
528


1
30.4
144506
424
2193
2194
2195
2196


1
40.2
36685
228
1405
1406
1407
1408


1
44
104827
336
1841
1842
1843
1844


1
45
151360
459
2333
2334
2335
2336


1
46.8
35417
216
1353
1354
1355
1356


1
46.8
37716
236
1437
1438
1439
1440


1
47.1
4409
25
585
586
587
588


1
52.2
42173
266
1561
1562
1563
1564


1
58.4
116
2
489
490
491
492


1
58.4
9159
52
693
694
695
696


1
60.3
143100
412
2145
2146
2147
2148


1
60.5
16876
113
937
938
939
940


1
60.6
33819
206
1313
1314
1315
1316


1
60.6
40124
254
1513
1514
1515
1516


1
60.6
40189
255
1517
1518
1519
1520


1
62.5
9449
57
713
714
715
716


1
62.5
33372
203
1301
1302
1303
1304


1
68.8
148156
451
2301
2302
2303
2304


1
83.2
25863
147
1073
1074
1075
1076


1
83.2
34205
207
1317
1318
1319
1320


1
83.2
43789
271
1581
1582
1583
1584


1
83.7
11522
74
781
782
783
784


1
83.7
106244
348
1889
1890
1891
1892


1
85.6
144090
423
2189
2190
2191
2192


1
85.9
5215
35
625
626
627
628


1
85.9
27375
152
1093
1094
1095
1096


1
85.9
69188
301
1701
1702
1703
1704


1
86.3
8984
49
681
682
683
684


1
86.3
36286
223
1385
1386
1387
1388


1
86.6
148194
452
2305
2306
2307
2308


1
88.8
29829
170
1165
1166
1167
1168


1
88.8
37068
231
1417
1418
1419
1420


1
88.8
68435
297
1685
1686
1687
1688


1
89.6
111365
399
2093
2094
2095
2096


1
90.5
60430
293
1669
1670
1671
1672


1
90.5
111828
405
2117
2118
2119
2120


1
91
113263
410
2137
2138
2139
2140


1
91.8
104474
333
1829
1830
1831
1832


1
92.3
145573
433
2229
2230
2231
2232


1
95
39351
247
1485
1486
1487
1488


1
96.4
107701
364
1953
1954
1955
1956


1
96.9
36448
225
1393
1394
1395
1396


1
99
40655
261
1541
1542
1543
1544


1
99
107077
357
1925
1926
1927
1928


1
103.3
8719
40
645
646
647
648


1
116.3
40338
257
1525
1526
1527
1528


1
116.3
54410
284
1633
1634
1635
1636


1
121.1
107621
362
1945
1946
1947
1948


1
121.5
16755
111
929
930
931
932


1
121.5
36863
230
1413
1414
1415
1416


1
122.1
41280
263
1549
1550
1551
1552


1
123.3
109328
379
2013
2014
2015
2016


1
124.6
33373
204
1305
1306
1307
1308


1
127.6
105648
346
1881
1882
1883
1884


1
129.5
4453
28
597
598
599
600


1
129.5
9626
64
741
742
743
744


1
129.5
37689
235
1433
1434
1435
1436


1
130.3
69565
302
1705
1706
1707
1708


1
132.1
34903
209
1325
1326
1327
1328


1
133.9
16724
102
893
894
895
896


1
138.5
12824
79
801
802
803
804


1
139.2
38701
241
1461
1462
1463
1464


1
140.8
5098
33
617
618
619
620


1
153.7
31993
191
1253
1254
1255
1256


1
156.7
8982
48
677
678
679
680


1
159.7
39502
248
1489
1490
1491
1492


1
160.4
148362
455
2317
2318
2319
2320


1
164.2
39896
252
1505
1506
1507
1508


1
165.6
108862
376
2001
2002
2003
2004


1
168.3
9701
67
753
754
755
756


1
178.6
151382
457
2325
2326
2327
2328


1
179.8
32253
198
1281
1282
1283
1284


1
194.2
13490
81
809
810
811
812


1
200.3
30840
181
1213
1214
1215
1216


1
207
16137
98
877
878
879
880


2
5.8
9867
71
769
770
771
772


2
5.8
31064
183
1221
1222
1223
1224


2
12.9
104447
332
1825
1826
1827
1828


2
14.1
39289
246
1481
1482
1483
1484


2
17.5
106678
351
1901
1902
1903
1904


2
19.5
82235
325
1797
1798
1799
1800


2
30.1
106842
353
1909
1910
1911
1912


2
32.8
2945
12
529
530
531
532


2
32.8
16074
97
873
874
875
876


2
33.9
80031
321
1781
1782
1783
1784


2
35.9
13691
89
841
842
843
844


2
35.9
50315
277
1605
1606
1607
1608


2
38.3
9706
68
757
758
759
760


2
42.4
32016
192
1257
1258
1259
1260


2
62.2
80704
323
1789
1790
1791
1792


2
70.4
9364
56
709
710
711
712


2
74.8
9623
63
737
738
739
740


2
75.6
40931
262
1545
1546
1547
1548


2
76.2
36323
224
1389
1390
1391
1392


2
76.2
104946
340
1857
1858
1859
1860


2
77.4
111617
403
2109
2110
2111
2112


2
78.2
11466
73
777
778
779
780


2
78.2
79073
315
1757
1758
1759
1760


2
78.2
108493
374
1993
1994
1995
1996


2
87.3
23442
143
1057
1058
1059
1060


2
87.5
107911
368
1969
1970
1971
1972


2
92.5
551
4
497
498
499
500


2
92.5
3177
14
537
538
539
540


2
92.5
53097
282
1625
1626
1627
1628


2
92.9
366
3
493
494
495
496


2
92.9
84829
330
1817
1818
1819
1820


2
99.7
151288
458
2329
2330
2331
2332


2
104.8
82458
327
1805
1806
1807
1808


2
106
111475
402
2105
2106
2107
2108


2
106.2
108013
371
1981
1982
1983
1984


2
107.6
2307
8
513
514
515
516


2
114.9
22775
135
1025
1026
1027
1028


2
123.4
104954
341
1861
1862
1863
1864


2
127
41850
264
1553
1554
1555
1556


2
134.9
31474
187
1237
1238
1239
1240


2
139.8
109207
377
2005
2006
2007
2008


2
144.2
35297
212
1337
1338
1339
1340


2
152.4
43579
270
1577
1578
1579
1580


2
153.5
147548
448
2289
2290
2291
2292


2
156.6
14467
84
821
822
823
824


2
157.2
33320
202
1297
1298
1299
1300


2
164.2
735
7
509
510
511
512


2
164.2
76792
308
1729
1730
1731
1732


3
6
8911
45
665
666
667
668


3
6
51614
280
1617
1618
1619
1620


3
9.1
10667
72
773
774
775
776


3
19.7
19963
117
953
954
955
956


3
19.7
32137
196
1273
1274
1275
1276


3
46.2
49293
275
1597
1598
1599
1600


3
52.3
109315
378
2009
2010
2011
2012


3
53.5
25000
144
1061
1062
1063
1064


3
54.1
21154
125
985
986
987
988


3
54.1
109722
384
2033
2034
2035
2036


3
57.2
109509
382
2025
2026
2027
2028


3
57.2
146158
435
2237
2238
2239
2240


3
57.5
107784
365
1957
1958
1959
1960


3
58.6
29867
173
1177
1178
1179
1180


3
59.3
4599
30
605
606
607
608


3
59.3
21190
131
1009
1010
1011
1012


3
59.3
28923
159
1121
1122
1123
1124


3
59.3
83776
328
1809
1810
1811
1812


3
59.3
147511
447
2285
2286
2287
2288


3
59.3
147768
449
2293
2294
2295
2296


3
60.4
8685
39
641
642
643
644


3
60.4
9468
58
717
718
719
720


3
60.4
9470
59
721
722
723
724


3
60.5
145322
432
2225
2226
2227
2228


3
61
16729
103
897
898
899
900


3
61.7
32247
197
1277
1278
1279
1280


3
61.7
39785
251
1501
1502
1503
1504


3
62.7
9144
51
689
690
691
692


3
62.7
9739
69
761
762
763
764


3
68.5
153431
460
2337
2338
2339
2340


3
68.5
154505
474
2393
2394
2395
2396


3
68.5
154509
477
2405
2406
2407
2408


3
68.5
154511
475
2397
2398
2399
2400


3
68.5
154532
472
2385
2386
2387
2388


3
68.5
154536
469
2373
2374
2375
2376


3
68.5
154552
471
2381
2382
2383
2384


3
68.5
154616
473
2389
2390
2391
2392


3
68.5
155689
479
2413
2414
2415
2416


3
68.5
155708
480
2417
2418
2419
2420


3
71
4886
31
609
610
611
612


3
71.5
24395
141
1049
1050
1051
1052


3
71.8
79081
316
1761
1762
1763
1764


3
74.3
23890
145
1065
1066
1067
1068


3
79.2
9173
53
697
698
699
700


3
89.7
15954
93
857
858
859
860


3
89.7
15965
94
861
862
863
864


3
93.2
77118
309
1733
1734
1735
1736


3
96.1
21772
129
1001
1002
1003
1004


3
96.5
36694
229
1409
1410
1411
1412


3
98.2
111204
397
2085
2086
2087
2088


3
98.6
29390
165
1145
1146
1147
1148


3
101.3
108630
375
1997
1998
1999
2000


3
105
35568
217
1357
1358
1359
1360


3
106.9
9473
60
725
726
727
728


3
109.4
21603
127
993
994
995
996


3
111.4
110780
392
2065
2066
2067
2068


3
118.4
146534
439
2253
2254
2255
2256


3
118.9
56939
287
1645
1646
1647
1648


3
123.8
143969
422
2185
2186
2187
2188


3
127.7
9079
50
685
686
687
688


3
139.1
3970
22
573
574
575
576


4
1
12340
77
793
794
795
796


4
23.6
2739
10
521
522
523
524


4
38.7
110069
386
2041
2042
2043
2044


4
38.7
111464
400
2097
2098
2099
2100


4
52.8
24647
146
1069
1070
1071
1072


4
53.2
40461
259
1533
1534
1535
1536


4
53.2
156243
484
2433
2434
2435
2436


4
58.6
1122
6
505
506
507
508


4
58.6
12012
75
785
786
787
788


4
62.1
10671
76
789
790
791
792


4
63.2
70730
305
1717
1718
1719
1720


4
64.9
38852
242
1465
1466
1467
1468


4
67.6
15096
90
845
846
847
848


4
67.6
154038
467
2365
2366
2367
2368


4
69.5
3351
19
561
562
563
564


4
69.5
5021
32
613
614
615
616


4
69.5
14666
88
837
838
839
840


4
69.5
15247
100
885
886
887
888


4
69.5
37503
232
1421
1422
1423
1424


4
69.5
80475
322
1785
1786
1787
1788


4
69.5
153424
464
2353
2354
2355
2356


4
69.9
107276
358
1929
1930
1931
1932


4
71.4
84527
329
1813
1814
1815
1816


4
73
35294
211
1333
1334
1335
1336


4
80
106845
354
1913
1914
1915
1916


4
86.5
3964
21
569
570
571
572


4
87.7
107840
366
1961
1962
1963
1964


4
92.1
9187
54
701
702
703
704


4
107.7
106491
350
1897
1898
1899
1900


4
108.2
39511
249
1493
1494
1495
1496


4
109.2
23289
138
1037
1038
1039
1040


4
109.7
26846
150
1085
1086
1087
1088


4
109.7
28933
161
1129
1130
1131
1132


4
110.3
8979
47
673
674
675
676


4
112.4
54460
285
1637
1638
1639
1640


4
115.1
71159
306
1721
1722
1723
1724


4
115.4
29435
166
1149
1150
1151
1152


4
117.6
30745
179
1205
1206
1207
1208


4
119.2
2435
9
517
518
519
520


4
119.2
12711
78
797
798
799
800


4
119.2
17828
108
917
918
919
920


4
119.2
18439
115
945
946
947
948


4
119.2
20933
121
969
970
971
972


4
119.2
20934
122
973
974
975
976


4
119.2
24422
142
1053
1054
1055
1056


4
120.9
29194
163
1137
1138
1139
1140


4
122.4
151472
456
2321
2322
2323
2324


4
128.1
32049
195
1269
1270
1271
1272


4
133.6
3224
16
545
546
547
548


4
133.6
3226
16
549
550
551
552


4
135.1
3152
13
533
534
535
536


4
135.1
4445
27
593
594
595
596


4
135.1
13833
82
813
814
815
816


4
135.8
17900
109
921
922
923
924


4
136.4
147219
443
2269
2270
2271
2272


4
142.1
147037
441
2261
2262
2263
2264


4
143.1
43121
268
1569
1570
1571
1572


4
144.8
35338
213
1341
1342
1343
1344


5
1.6
24265
140
1045
1046
1047
1048


5
1.6
31790
190
1249
1250
1251
1252


5
9.7
143251
414
2153
2154
2155
2156


5
13.8
69592
303
1709
1710
1711
1712


5
16.7
57137
288
1649
1650
1651
1652


5
16.7
105613
345
1877
1878
1879
1880


5
17.2
107858
367
1965
1966
1967
1968


5
27.4
91
1
485
486
487
488


5
31.3
5275
36
629
630
631
632


5
39.9
109403
381
2021
2022
2023
2024


5
41.7
16527
99
881
882
883
884


5
50.9
109342
380
2017
2018
2019
2020


5
51.9
16762
104
901
902
903
904


5
51.9
16767
105
905
906
907
908


5
53.4
79519
318
1769
1770
1771
1772


5
56.5
9668
66
749
750
751
752


5
57.7
30270
177
1197
1198
1199
1200


5
57.7
52081
281
1621
1622
1623
1624


5
57.7
77545
311
1741
1742
1743
1744


5
62.3
51419
279
1613
1614
1615
1616


5
63
32272
199
1285
1286
1287
1288


5
66.9
30000
174
1181
1182
1183
1184


5
66.9
146415
437
2245
2246
2247
2248


5
69.3
106912
355
1917
1918
1919
1920


5
69.6
107061
356
1921
1922
1923
1924


5
69.6
144731
426
2201
2202
2203
2204


5
70.5
105854
347
1885
1886
1887
1888


5
70.8
22796
136
1029
1030
1031
1032


5
70.8
27874
158
1117
1118
1119
1120


5
71.7
143216
413
2149
2150
2151
2152


5
73.2
33249
201
1293
1294
1295
1296


5
76.4
29820
169
1161
1162
1163
1164


5
78.2
144687
425
2197
2198
2199
2200


5
80.2
36637
227
1401
1402
1403
1404


5
80.9
143418
419
2173
2174
2175
2176


5
81.2
38478
238
1449
1450
1451
1452


5
81.5
48616
274
1593
1594
1595
1596


5
83
104850
337
1845
1846
1847
1848


5
83.7
148026
450
2297
2298
2299
2300


5
86.2
9297
55
705
706
707
708


5
87.3
106300
349
1893
1894
1895
1896


5
90.6
5480
38
637
638
639
640


5
100.9
35377
214
1345
1346
1347
1348


5
104.5
58375
290
1657
1658
1659
1660


5
117.1
143380
416
2161
2162
2163
2164


5
136
105546
343
1869
1870
1871
1872


5
150.5
31084
184
1225
1226
1227
1228


6
16.4
27615
157
1113
1114
1115
1116


6
17.3
154854
476
2401
2402
2403
2404


6
19.4
105014
342
1865
1866
1867
1868


6
22.9
79529
319
1773
1774
1775
1776


6
30.5
3284
18
557
558
559
560


6
30.5
69630
304
1713
1714
1715
1716


6
32.8
29780
168
1157
1158
1159
1160


6
32.8
68941
298
1689
1690
1691
1692


6
32.8
146458
438
2249
2250
2251
2252


6
33.3
104510
334
1833
1834
1835
1836


6
33.3
107639
363
1949
1950
1951
1952


6
35.3
15304
101
889
890
891
892


6
35.3
16944
106
909
910
911
912


6
35.3
35574
218
1361
1362
1363
1364


6
35.3
77413
310
1737
1738
1739
1740


6
35.3
110607
389
2053
2054
2055
2056


6
37.3
36067
221
1373
1374
1375
1376


6
37.3
36073
221
1377
1378
1379
1380


6
41.2
34560
208
1321
1322
1323
1324


6
43.1
30176
176
1193
1194
1195
1196


6
52.8
4463
29
601
602
603
604


6
53.1
60751
294
1673
1674
1675
1676


6
53.5
32034
194
1265
1266
1267
1268


6
53.5
57758
289
1653
1654
1655
1656


6
53.5
108212
372
1985
1986
1987
1988


6
58.1
59008
292
1665
1666
1667
1668


6
58.1
146195
436
2241
2242
2243
2244


6
59.9
3277
17
553
554
555
556


6
59.9
105586
344
1873
1874
1875
1876


6
61.5
148039
453
2309
2310
2311
2312


6
61.5
155861
482
2425
2426
2427
2428


6
63.1
20410
128
997
998
999
1000


6
66.6
8838
43
657
658
659
660


6
67.5
14694
96
869
870
871
872


6
73.3
113381
411
2141
2142
2143
2144


6
86.9
110972
395
2077
2078
2079
2080


6
92.8
37947
237
1441
1442
1443
1444


6
92.8
37948
237
1445
1446
1447
1448


6
93.9
5319
37
633
634
635
636


6
95.8
30771
180
1209
1210
1211
1212


6
97.8
27295
151
1089
1090
1091
1092


6
99.1
108433
373
1989
1990
1991
1992


6
110.4
31684
189
1245
1246
1247
1248


6
112.4
107449
361
1941
1942
1943
1944


6
117.4
16017
107
913
914
915
916


6
121
37634
234
1429
1430
1431
1432


6
128.7
9667
65
745
746
747
748


6
130.8
21433
126
989
990
991
992


6
132.7
37555
233
1425
1426
1427
1428


7
56.2
68954
299
1693
1694
1695
1696


7
62
31370
186
1233
1234
1235
1236


7
62
42164
265
1557
1558
1559
1560


7
67
30674
178
1201
1202
1203
1204


7
67.4
33769
205
1309
1310
1311
1312


7
67.9
39064
244
1473
1474
1475
1476


7
69.1
35633
219
1365
1366
1367
1368


7
72.8
42930
267
1565
1566
1567
1568


7
73.8
68426
296
1681
1682
1683
1684


7
73.9
29005
162
1133
1134
1135
1136


7
74
51405
278
1609
1610
1611
1612


7
83.6
29362
164
1141
1142
1143
1144


7
98.5
8799
42
653
654
655
656


7
98.8
48425
272
1585
1586
1587
1588


7
99.8
4415
26
589
590
591
592


7
99.8
35408
215
1349
1350
1351
1352


7
103.6
79695
320
1777
1778
1779
1780


7
107.5
38914
243
1469
1470
1471
1472


7
109.5
39978
253
1509
1510
1511
1512


7
113.3
155829
481
2421
2422
2423
2424


7
114.7
28932
160
1125
1126
1127
1128


7
114.7
31547
188
1241
1242
1243
1244


7
114.7
68149
295
1677
1678
1679
1680


7
115.8
4093
23
577
578
579
580


7
118.6
4302
24
581
582
583
584


7
118.6
38653
240
1457
1458
1459
1460


7
118.6
78828
314
1753
1754
1755
1756


7
118.6
81460
324
1793
1794
1795
1796


7
120.9
153856
463
2349
2350
2351
2352


7
122.2
145260
429
2213
2214
2215
2216


7
124.5
15184
92
853
854
855
856


7
124.5
39773
250
1497
1498
1499
1500


7
131.6
79307
317
1765
1766
1767
1768


7
132.8
30026
175
1185
1186
1187
1188


7
132.8
30029
175
1189
1190
1191
1192


7
144.6
30872
182
1217
1218
1219
1220


7
154.5
110771
391
2061
2062
2063
2064


7
164.7
155475
478
2409
2410
2411
2412


7
165.5
146593
440
2257
2258
2259
2260


7
167
143371
415
2157
2158
2159
2160


7
186.5
36490
226
1397
1398
1399
1400


7
189.3
18157
112
933
934
935
936


7
189.4
21038
124
981
982
983
984


7
189.4
69120
300
1697
1698
1699
1700


7
189.4
71624
307
1725
1726
1727
1728


7
193.9
19704
123
977
978
979
980


8
8.8
35173
210
1329
1330
1331
1332


8
16.4
40320
256
1521
1522
1523
1524


8
16.9
19198
116
949
950
951
952


8
34.3
29842
172
1173
1174
1175
1176


8
40.9
107937
369
1973
1974
1975
1976


8
43.1
111628
404
2113
2114
2115
2116


8
43.3
26720
154
1101
1102
1103
1104


8
45.5
32030
193
1261
1262
1263
1264


8
47.3
53899
283
1629
1630
1631
1632


8
47.9
104862
339
1853
1854
1855
1856


8
49.7
107396
360
1937
1938
1939
1940


8
53.9
27361
156
1109
1110
1111
1112


8
53.9
145200
428
2209
2210
2211
2212


8
55.7
23091
137
1033
1034
1035
1036


8
59.3
77568
312
1745
1746
1747
1748


8
64
110148
387
2045
2046
2047
2048


8
64.5
104858
338
1849
1850
1851
1852


8
65.8
104389
331
1821
1822
1823
1824


8
66.6
21895
130
1005
1006
1007
1008


8
67.4
48562
273
1589
1590
1591
1592


8
68.4
82295
326
1801
1802
1803
1804


8
76.8
111472
401
2101
2102
2103
2104


8
85.9
110684
390
2057
2058
2059
2060


8
87.5
9759
70
765
766
767
768


8
87.5
20537
120
965
966
967
968


8
87.5
112497
408
2129
2130
2131
2132


8
105.5
107286
359
1933
1934
1935
1936


8
106.8
13100
80
805
806
807
808


8
117.3
145077
427
2205
2206
2207
2208


8
117.3
145298
430
2217
2218
2219
2220


8
132.8
14545
87
833
834
835
836


8
135.5
8757
41
649
650
651
652


8
137.4
561
5
501
502
503
504


9
0
14479
86
829
830
831
832


9
20.5
58904
291
1661
1662
1663
1664


9
38.1
49557
276
1601
1602
1603
1604


9
78.2
29745
167
1153
1154
1155
1156


9
87.7
12557
83
817
818
819
820


9
90.3
23779
139
1041
1042
1043
1044


9
93.4
29832
171
1169
1170
1171
1172


9
93.4
55370
286
1641
1642
1643
1644


9
93.8
110377
388
2049
2050
2051
2052


9
93.8
113113
409
2133
2134
2135
2136


9
93.9
155159
470
2377
2378
2379
2380


9
94
25961
148
1077
1078
1079
1080


9
94.5
148621
454
2313
2314
2315
2316


9
94.6
112139
407
2125
2126
2127
2128


9
94.7
31233
185
1229
1230
1231
1232


9
94.7
153885
465
2357
2358
2359
2360


9
100.6
20048
118
957
958
959
960


9
100.6
153427
461
2341
2342
2343
2344


9
108.3
109802
385
2037
2038
2039
2040


9
108.9
155793
483
2429
2430
2431
2432


9
110.3
8937
46
669
670
671
672


9
110.3
78438
313
1749
1750
1751
1752


9
111.2
147496
446
2281
2282
2283
2284


9
112.4
13086
85
825
826
827
828


9
115
145318
431
2221
2222
2223
2224


9
125.2
9555
62
733
734
735
736


9
137.2
36022
220
1369
1370
1371
1372


9
145.8
110800
393
2069
2070
2071
2072


9
165.8
110886
394
2073
2074
2075
2076


9
171.4
147417
445
2277
2278
2279
2280


10
23.9
153632
462
2345
2346
2347
2348


10
23.9
153987
466
2361
2362
2363
2364


10
23.9
154021
468
2369
2370
2371
2372


10
29.7
20502
119
961
962
963
964


10
31.7
104672
335
1837
1838
1839
1840


10
36.9
111004
396
2081
2082
2083
2084


10
43.4
16041
95
865
866
867
868


10
50.5
143408
418
2169
2170
2171
2172


10
51.1
147411
444
2273
2274
2275
2276


10
52.7
143754
421
2181
2182
2183
2184


10
54.7
5140
34
621
622
623
624


10
55.6
111212
398
2089
2090
2091
2092


10
55.8
8840
44
661
662
663
664


10
55.8
21292
132
1013
1014
1015
1016


10
55.8
22541
133
1017
1018
1019
1020


10
55.8
143388
417
2165
2166
2167
2168


10
56.1
39275
245
1477
1478
1479
1480


10
56.7
22717
134
1021
1022
1023
1024


10
57
3206
15
541
542
543
544


10
57
32428
200
1289
1290
1291
1292


10
65.5
3640
20
565
566
567
568


10
66.5
16730
110
925
926
927
928


10
68
107941
370
1977
1978
1979
1980


10
71.6
18392
114
941
942
943
944


10
73.6
27447
153
1097
1098
1099
1100


10
77.4
13745
91
849
850
851
852


10
83.9
38604
239
1453
1454
1455
1456


10
85.9
40431
258
1529
1530
1531
1532


10
85.9
40474
260
1537
1538
1539
1540


10
89.6
106742
352
1905
1906
1907
1908


10
93.2
143657
420
2177
2178
2179
2180


10
93.2
145800
434
2233
2234
2235
2236


10
96.4
9486
61
729
730
731
732


10
100.9
109666
383
2029
2030
2031
2032









Example 2
This example illustrates a labeled probe degradation assay for SNP detection and marker mapping.
A quantity of maize genomic template DNA (e.g., about 2-20 ng) is mixed in 5 μLl total volume with four oligonucleotides, which can be designed by Applied Biosystems, i.e., a forward primer, a reverse primer, a hybridization probe having a VIC reporter attached to the 5′ end, and a hybridization probe having a FAM reporter attached to the 5′end as well as PCR reaction buffer containing the passive reference dye ROX. The PCR reaction is conducted for 35 cycles using a 60° C. annealing-extension temperature. Following the reaction, the fluorescence of each fluorophore as well as that of the passive reference is determined in a fluorimeter. The fluorescence value for each fluorophore is normalized to the fluorescence value of the passive reference. The normalized values are plotted against each other for each sample, producing an allelogram as described above. As described above, the data points should fall into clearly separable clusters.
To confirm that an assay produces accurate results, each new assay is performed on a number of replicates of samples of known genotypic identity representing each of the three possible genotypes, i.e., two homozygous alleles and a heterozygous sample. To be a valid and useful assay, it must produce clearly separable clusters of data points, such that one of the three genotypes can be assigned for at least 90% of the data points, and the assignment is observed to be correct for at least 98% of the data points. Subsequent to this validation step, the assay is applied to progeny of a cross between two highly inbred individuals to obtain segregation data, which are then used to calculate a genetic map position for the polymorphic locus.
The maize markers were genetically mapped based on the genotypes of certain SNPs. The genotypes were combined with genotypes for public core SSR and RFLP markers scored on recombinant inbred lines. Before mapping, any loci showing distorted segregation (P<0.01 for a Chi-square test of a 1:1 segregation ratio) were removed. These loci could be added to the map later but without allowing them to change marker order. A map was constructed using the JoinMap version 2.0 software, which is described by Stam (“Construction of integrated genetic linkage maps by means of a new computer package: JoinMap, The Plant Journal, 3: 739-744 (1993); Stam, P. and van Ooijen, J. W. “JoinMap version 2.0: Software for the calculation of genetic linkage maps (1995) CPRO-DLO, Wageningen). JoinMap implements a weighted-least squares approach to multipoint mapping in which information from all pairs of linked loci (adjacent or not) is incorporated. Linkage groups were formed using a LOD threshold of 5.0. The SSR and RFLP public markers were used to assign linkage groups to chromosomes. Linkage groups were merged within chromosomes before map construction.
Haldane's mapping function was used to convert recombination fractions to map distances. Lenient criteria was applied for excluding pairwise linkage data; only data with a LOD not greater than 0.001 or a recombination fraction not less than 0.499 are excluded. Parameters for ordering loci were a jump threshold of 5.0, a triplet threshold of 7.0 and a ripple value of 3. About 38% of the loci were ordered in two rounds of map construction with a jump threshold of 5.0, which prevents the addition of a locus to the map if such addition results in a jump of more than 5.0 to a goodness-of-fit criterion. The remaining loci were added to the map without application of such a jump threshold. Addition of these loci had a negligible effect on the map order and distances for the initial loci. Mapped SNP polymorphisms are identified in Table 2.
Example 3
This example illustrates the utility of the markers in marker trait association
The 488 maize markers of this invention were used in an association study to identify which of the candidate genes were more significantly associated with oil level in corn (Zea mays).
The University of Illinois has corn lines differing in seed oil that have been developed by long-term selection. A high oil line (IHO) produces about 18% seed oil and a low oil line (ILO) produces about 1.5% seed oil. The IHO and ILO lines are available from the University of Illinois for research. A random mated population (RMn) was produced from random mating offspring of a cross between IHO and ILO by chain crossing for 10 generations to produce an RM10 population. From the RM10 population 504 S1-derived lines were developed by selfing and these lines constitute an association study population. This population along with 72 control samples were genotyped using oil informative SNPs.
Phenotypes were measured on 504 association population lines in replicated field trials with an alph(0,1) incomplete block design. The field trials comprised the 504 lines grown in each of two years at each of 3 locations with 2 replicates per location. The lines were blocked within each replicate. These field trials were performed on the 504 RM10:S1 lines, per se, and on hybrids made by crossing each line to a tester line, i.e., line (7051).
Association was analyzed between the SNP markers and oil level in the RM10:S1 lines, per se, and in the hybrids. A mixed model analysis of variance was performed with sources of variation: location, reps within location, blocks and lines. Line effects estimated from this model were regressed on single marker genotypes (i.e., number of A alleles in the genotypes AA, Aa and aa). The probability that the slope is significantly different from zero gives an indication of whether the marker has a significant effect on the trait. Through this analysis of percent oil in the kernel and oil per 200 kernels in both inbreds and hybrids, a total of 186 markers showed significance at the p<0.05 level. These 186 significant markers are very likely to either reside within an oil gene or to be closely linked to an oil gene and are more particularly described in Table 3 by:
“Map Position” which identifies the distance measured in cM from the 5′ end of a maize chromosome for the SNP identified by “Mutation ID”, which refers to an arbitrary identifying name for each polymorphism;
Pval % Oil Per se, which refers to probability of a test of significance of the regression of marker genotype on oil level as percent oil per kernel for inbred lines;
Pval % Oil Hybrid, which refers to probability of a test of significance of the regression of marker genotype on oil level as percent oil per kernel for hybrid lines.
Pval Oil/Kernel Per se, which refers to probability of a test of significance of the regression of marker genotype on oil level as oil weight per 200 kernels for inbred lines;
Pval Oil/Kernel Hybrid, which refers to probability of a test of significance of the regression of marker genotype on oil level as oil weight per 200 kernels for hybrid lines












TABLE 3









Pval
Pval


Map

Pval % Oil
Pval % Oil
Oil/Kernel
Oil/Kernel


Position
Mutation_ID
Per se
Hybrid
Per se
Hybrid




















1-3.7
111829
0.706
0.234
0.336
0.046


1-25.1
43230
0.030
0.228
0.042
0.037


1-44
104827
0.094
0.801
0.018
0.909


1-45
151360
0.025
0.811
0.005
0.395


1-46.8
37716
0.009
0.113
0.024
0.351


1-53.3
42173
0.020
0.050
0.024
0.907


1-58.4
116
0.059
0.018
0.018
0.395


1-60.3
143100
0.722
0.029
0.878
0.501


1-60.6
33819
0.200
0.039
0.043
0.640


1-60.6
40189
0.007
1.6E−4
0.062
0.172


1-83.2
34205
0.026
0.151
0.090
0.022


1-86.3
8984
0.405
8.0E−4
0.433
0.069


1-86.3
36286
0.261
7.3E−4
0.328
0.069


1-88.8
29829
0.063
0.164
0.597
0.029


1-88.8
37068
0.026
0.317
0.068
0.051


1-90.5
111828
0.052
0.198
0.018
0.014


1-91
113263
0.281
0.004
0.078
0.489


1-91.8
104474
0.047
0.346
0.776
0.069


1-96.9
36448
0.006
0.114
0.002
0.052


1-99
40655
0.029
0.272
0.052
0.080


1-99
107077
9.7E−6
0.014
9.1E−4
0.021


1-103.3
8719
0.167
0.728
0.008
0.271


1-124.6
33373
0.029
0.240
0.201
0.714


1-130.3
69565
0.032
0.201
0.568
0.962


1-165.6
108862
0.011
0.001
0.402
0.347


1-178.6
151382
0.027
0.480
0.116
0.509


1-200.3
30840
0.662
0.050
0.716
0.012


2-5.8
31064
0.091
0.002
0.143
0.064


2-12.9
104447
0.077
0.012
0.697
0.459


2-14.1
39289
0.095
0.016
0.778
0.571


2-17.5
106678
0.048
0.003
0.043
0.040


2-19.5
82235
0.018
0.002
0.045
0.009


2-33.9
80031
0.101
0.046
0.557
0.036


2-35.9
13691
0.225
0.469
0.040
0.419


2-78.2
11466
0.096
0.761
0.045
0.225


2-78.2
79073
0.020
0.825
0.015
0.413


2-78.2
108493
0.142
0.045
0.713
0.299


2-92.5
3177
0.082
0.334
0.038
0.224


2-92.9
84829
0.298
0.324
0.111
0.031


2-99.7
151288
0.549
0.036
0.245
0.846


2-106
111475
0.238
0.013
0.320
0.685


2-106.2
108013
0.574
0.033
0.441
0.591


2-107.6
2307
0.497
0.019
0.437
0.413


2-114.9
22775
0.036
0.064
0.424
0.160


2-123.4
104954
0.049
0.058
0.573
0.765


2-152.4
43579
0.064
0.123
0.037
0.659


2-164.2
735
0.497
0.920
0.048
0.729


2-164.2
76792
0.939
0.524
0.044
0.345


3-6
8911
0.067
0.561
0.045
0.979


3-6
51614
0.071
0.551
0.030
0.980


3-9.1
10667
0.009
0.193
0.068
0.262


3-19.7
19963
0.115
0.084
0.029
0.373


3-19.7
32137
2.4E−4
1.1E−4
2.3E−4
0.037


3-46.2
49293
0.036
0.003
0.167
0.030


3-52.3
109315
0.175
0.7E−4
0.527
0.040


3-53.5
25000
0.098
4.5E−4
0.350
0.157


3-54.1
21154
0.060
7.7E−4
0.543
0.542


3-54.1
109722
0.482
0.022
0.526
0.284


3-57.2
109509
0.394
0.006
0.464
0.213


3-58.6
29867
0.036
1.8E−8
0.696
0.169


3-59.3
4599
0.093
7.9E−4
0.562
0.571


3-59.3
21190
0.020
0.006
0.637
0.215


3-59.3
28923
0.150
9.6E−4
0.703
0.351


3-59.3
147511
0.116
0.001
0.588
0.571


3-59.3
147768
0.066
7.6E−4
0.627
0.524


3-60.4
8685
0.592
0.001
0.913
0.681


3-61
16729
0.229
0.112
0.198
0.005


3-61.7
32247
0.115
3.5E−4
0.891
0.113


3-62.7
9144
0.066
0.003
0.277
0.014


3-62.7
9739
0.031
0.003
0.439
0.130


3-111.4
110780
0.246
0.040
0.572
0.207


3-123.8
143969
0.015
0.158
0.081
0.438


3-127.7
9079
0.040
0.071
0.296
0.134


4-38.7
110069
0.026
0.048
0.188
0.108


4-38.7
111464
0.029
0.053
0.129
0.096


4-52.8
24647
0.013
0.084
0.382
0.827


4-53.2
156243
0.004
0.007
0.096
0.368


4-62.1
10671
0.156
0.040
0.337
0.099


4-64.9
38852
0.285
0.072
0.342
0.007


4-69.5
5021
0.341
0.499
0.098
0.004


4-69.5
37503
0.262
0.126
0.303
0.002


4-69.9
107276
0.006
0.331
0.017
0.016


4-71.4
84527
0.346
0.014
0.363
0.040


4-80
106845
0.112
0.042
0.393
0.434


4-107.7
106491
0.020
0.040
0.409
0.521


4-112.4
54460
0.037
0.146
0.124
0.150


4-122.4
151472
0.186
0.994
0.011
0.967


4-128.1
32049
0.195
0.620
0.756
0.011


4-135.8
17900
4.2E−4
0.037
3.7E−4
0.019


4-136.4
147219
0.038
0.214
0.104
0.029


5-1.6
24265
0.082
0.035
0.472
0.010


5-39.9
109403
2.0E−6
9.1E−5
0.135
0.006


5-41.7
16527
0.028
0.161
0.333
0.791


5-50.9
109342
0.005
0.167
0.015
0.024


5-51.9
16762
7.6E−5
0.018
4.9E−4
0.029


5-51.9
16767
1.2E−4
0.017
5.1E−4
0.033


5-62.3
51419
0.046
0.002
0.163
0.031


5-63
32272
5.7E−5
0.001
0.008
0.100


5-66.9
30000
0.004
6.5E−4
0.035
0.002


5-66.9
146415
1.1E−4
1.9E−5
0.163
0.037


5-69.6
144731
8.5E−4
2.8E−4
0.162
0.042


5-70.5
105854
0.205
0.011
0.976
0.063


5-71.7
143216
0.023
0.014
0.065
0.098


5-76.4
29820
0.010
5.8E−4
0.128
0.140


5-80.2
36637
0.020
0.052
0.087
0.365


5-104.5
58375
0.028
0.097
0.024
0.003


5-150.5
31084
0.025
0.210
0.350
0.763


6-17.3
154854
0.010
0.222
0.049
0.688


6-30.8
69630
0.484
0.678
0.094
0.047


6-37.3
36067
0.018
0.290
0.215
0.874


6-37.3
36073
0.014
0.165
0.234
0.945


6-43.1
30176
0.827
0.323
0.969
0.015


6-52.8
4463
3.9E−9
1.8E−12
2.3E−6
3.7E−9


6-53.1
60751
3.9E−9
1.6E−6
5.4E−7
2.2E−4


6-53.5
32034
5.0E−7
3.7E−4
5.3E−5
0.048


6-53.5
57758
6.3E−5
0.008
0.002
0.566


6-53.5
108212
8.6E−7
6.0E−4
4.0E−5
0.043


6-58.1
59008
9.7E−5
3.9E−4
8.2E−5
0.002


6-58.1
146195
5.3E−4
8.5E−4
1.8E−5
0.004


6-59.9
3277
0.004
0.215
0.087
0.515


6-59.9
105586
0.003
4.9E−4
0.002
0.001


6-61.5
148039
0.120
7.6E−4
0.565
0.006


6-61.5
155861
0.082
7.2E−4
0.490
0.003


6-63.1
20410
0.028
0.012
0.055
0.138


6-66.6
8838
0.050
0.009
0.031
0.025


6-67.5
14694
0.226
8.2E−4
0.496
0.151


6-86.9
110972
0.023
0.050
0.072
0.482


6-110.4
31684
0.012
9.6E−4
0.162
0.240


6-121
37634
0.002
0.052
0.052
0.008


6-132.7
37555
0.089
0.364
0.665
0.025


7-62
42164
0.075
0.424
0.045
0.235


7-67
30674
0.424
0.048
0.187
0.015


7-68.7
39064
0.321
0.558
0.028
0.357


7-72.8
42930
0.111
0.076
0.006
0.002


7-74.2
68426
0.013
0.662
0.047
0.088


7-98.5
8799
0.031
0.429
0.009
0.160


7-98.8
48425
7.4E−4
0.063
2.6E−4
0.034


7-99.8
4415
6.9E−4
0.057
1.5E−4
0.032


7-99.8
35408
0.003
0.055
0.002
0.069


7-107.5
38914
0.024
0.002
0.682
0.747


7-115.8
4093
0.185
0.007
0.512
0.050


7-118.6
4302
0.032
6.5E−4
0.522
0.120


7-118.6
38653
0.199
0.011
0.471
0.035


7-118.6
81460
0.061
0.002
0.578
0.257


7-122.2
145260
0.062
0.003
0.108
0.003


7-124.5
15184
0.044
0.009
0.079
0.008


7-124.5
39773
0.065
0.022
0.814
0.608


7-132.8
30029
0.330
0.046
0.577
0.552


8-16.4
40320
0.657
0.063
0.405
0.006


8-40.9
107937
0.048
0.046
0.221
0.077


8-43.1
111628
0.105
0.011
0.401
0.144


8-45.5
26720
0.109
0.043
0.459
0.282


8-47.9
104862
0.152
0.143
0.011
0.276


8-53.9
27361
0.798
0.947
0.378
0.048


8-53.9
145200
0.260
0.129
0.033
0.016


8-55.7
23091
0.040
0.183
0.069
0.872


8-59.3
77568
0.003
0.258
4.4E−5
0.249


8-64
110148
0.005
0.085
0.005
0.528


8-65.8
104389
0.003
0.117
0.004
0.415


8-66.6
21895
0.003
0.143
0.002
0.417


8-67.4
48562
0.006
0.081
0.005
0.416


8-68.4
82295
0.003
0.311
4.9E−4
0.067


8-85.9
110684
0.039
0.588
0.030
0.082


8-87.5
9759
0.473
0.574
0.010
0.353


8-105.5
107286
0.028
0.063
0.104
0.076


8-106.8
13100
0.030
0.068
0.107
0.043


8-117.3
145077
0.006
0.009
0.055
0.192


8-117.3
145298
0.005
0.004
0.076
0.210


9-20.5
58904
0.578
0.351
0.078
0.041


9-80
29745
0.182
0.025
0.538
0.116


9-93.8
110377
0.021
0.097
0.019
0.134


9-93.8
113113
0.022
0.098
0.026
0.290


9-94
25961
0.058
0.047
0.039
0.177


9-94.5
148621
0.060
0.300
0.030
0.066


9-100.6
20048
0.034
0.137
0.155
0.156


9-100.6
153427
0.022
0.154
0.092
0.101


9-110.3
8937
0.014
0.652
0.035
0.233


9-125.2
9555
0.419
0.270
0.307
0.049


9-137.2
36022
0.136
0.014
0.290
0.096


10-52.7
143754
0.608
0.127
0.648
0.044


10-56.1
39275
0.061
0.019
0.054
0.085


10-89.6
106742
0.064
0.105
0.035
0.088


10-93.2
143657
0.560
0.046
0.790
0.186


10-93.2
145800
0.551
0.030
0.793
0.190


10-100.9
109666
0.083
0.007
0.144
0.148


unmapped
152577
0.315
0.001
0.627
0.914


unmapped
20742
0.425
0.141
0.435
0.041
































SEQ_

MUTA-
START_
END_











NUM
SEQ_ID
TION_ID
POS
POS
TYPE
ALLELE1
STRAINS1
ALLELE2
STRAINS2
ALLELE3
STRAINS3
ALLELE4
STRAINS4




























1
Amplicon25
90
106
106
SNP
A
T








1
Amplicon25
91
110
110
SNP
C
T


1
Amplicon25
92
130
130
SNP
A
T


1
Amplicon25
93
175
175
SNP
C
T


1
Amplicon25
94
185
185
SNP
A
G


1
Amplicon25
95
189
189
SNP
A
G


1
Amplicon25
96
279
279
SNP
A
C


1
Amplicon25
97
300
300
SNP
C
G


2
Amplicon29
114
68
68
SNP
A
C


2
Amplicon29
115
101
101
SNP
G
T


2
Amplicon29
116
283
283
SNP
A
G


3
Amplicon78
366
145
145
SNP
A
G


4
Amplicon150
548
84
84
SNP
A
C


4
Amplicon150
549
107
107
SNP
C
T


4
Amplicon150
550
157
157
SNP
A
T


4
Amplicon150
551
174
174
SNP
G
T


5
Amplicon155
561
70
70
SNP
A
C


6
Amplicon161
1122
215
215
SNP
C
T


6
Amplicon161
1123
233
233
SNP
C
T


7
Amplicon213
734
11
11
SNP
C
T


7
Amplicon213
735
93
93
SNP
A
G


7
Amplicon213
736
210
210
SNP
A
G


7
Amplicon213
737
216
216
SNP
G
T


7
Amplicon213
738
233
238
IND
******
ttgagt


8
Amplicon49714
2300
59
59
SNP
A
b73
G
mo17


8
Amplicon49714
2301
172
172
SNP
G
mo17
T
b73


8
Amplicon49714
2302
182
182
SNP
C
mo17
T
b73


8
Amplicon49714
2303
221
221
SNP
C
mo17
T
b73


8
Amplicon49714
2304
230
230
SNP
C
b73
T
mo17


8
Amplicon49714
2305
308
308
SNP
C
mo17
G
b73


8
Amplicon49714
2306
344
344
SNP
A
b73
G
mo17


8
Amplicon49714
2307
378
378
SNP
A
b73
G
mo17


8
Amplicon49714
2308
389
389
SNP
C
b73
G
mo17


8
Amplicon49714
2309
424
424
SNP
C
b73
T
mo17


8
Amplicon49714
2310
446
446
SNP
C
mo17
T
b73


9
Amplicon49778
2435
355
355
SNP
C
mo17
T
b73


10
Amplicon49935
2739
133
133
IND
*
b73
C
mo17


11
Amplicon49980
2841
158
158
SNP
C
b73
T
mo17


11
Amplicon49980
2842
215
215
SNP
C
mo17
T
b73


11
Amplicon49980
2843
303
303
SNP
A
mo17
C
b73


11
Amplicon49980
2844
304
304
SNP
C
mo17
T
b73


11
Amplicon49980
2845
380
380
SNP
G
mo17
T
b73


11
Amplicon49980
2846
434
434
SNP
C
mo17
T
b73


11
Amplicon49980
2847
445
445
SNP
G
mo17
T
b73


11
Amplicon49980
2848
466
466
SNP
A
mo17
G
b73


12
Amplicon50024
2941
99
99
SNP
C
b73
T
mo17


12
Amplicon50024
2942
127
127
SNP
A
b73
T
mo17


12
Amplicon50024
2943
169
169
SNP
A
b73
G
mo17


12
Amplicon50024
2944
234
240
IND
*******
mo17
GGCTACA
b73


12
Amplicon50024
2945
255
255
SNP
A
mo17
G
b73


12
Amplicon50024
2946
270
270
SNP
A
mo17
T
b73


12
Amplicon50024
2947
304
304
SNP
C
b73
T
mo17


12
Amplicon50024
2948
351
351
SNP
A
mo17
G
b73


12
Amplicon50024
2949
361
361
SNP
G
b73
T
mo17


12
Amplicon50024
2950
363
363
SNP
G
b73
T
mo17


12
Amplicon50024
2951
378
378
SNP
C
b73
T
mo17


12
Amplicon50024
2952
398
398
SNP
A
b73
C
mo17


12
Amplicon50024
2953
401
401
SNP
C
mo17
T
b73


12
Amplicon50024
2954
406
406
SNP
C
mo17
G
b73


12
Amplicon50024
2955
417
417
SNP
C
mo17
G
b73


12
Amplicon50024
2956
442
442
SNP
A
mo17
G
b73


12
Amplicon50024
2957
477
477
SNP
G
b73
T
mo17


12
Amplicon50024
2958
503
503
SNP
C
b73
T
mo17


12
Amplicon50024
2959
514
514
IND
*
mo17
T
b73


12
Amplicon50024
2960
556
556
SNP
C
b73
G
mo17


12
Amplicon50024
2961
557
557
IND
*
b73
G
mo17


12
Amplicon50024
2962
559
559
IND
*
b73
C
mo17


13
Amplicon50099
3152
290
290
SNP
C
b73
G
mo17


14
Amplicon50106
3172
94
94
SNP
A
b73
C
mo17


14
Amplicon50106
3173
187
187
SNP
A
b73
G
mo17


14
Amplicon50106
3174
243
243
IND
*
b73
A
mo17


14
Amplicon50106
3175
243
244
IND
**
b73
AT
mo17


14
Amplicon50106
3176
283
283
SNP
A
b73
G
mo17


14
Amplicon50106
3177
415
416
IND
**
mo17
TC
b73


14
Amplicon50106
3178
438
438
SNP
C
mo17
T
b73


14
Amplicon50106
3179
442
442
SNP
C
b73
T
mo17


14
Amplicon50106
3180
443
443
SNP
C
b73
G
mo17


15
Amplicon50125
3205
215
215
SNP
A
mo17
G
b73


15
Amplicon50125
3206
256
256
SNP
C
mo17
T
b73


16
Amplicon50142
3222
104
104
SNP
C
b73
T
mo17


16
Amplicon50142
3223
110
110
SNP
C
b73
T
mo17


16
Amplicon50142
3224
171
171
SNP
C
mo17
G
b73


16
Amplicon50142
3225
372
374
IND
***
b73
CGG
mo17


16
Amplicon50142
3226
401
401
SNP
C
mo17
T
b73


17
Amplicon50179
3277
83
83
SNP
C
mo17
T
b73


17
Amplicon50179
3278
265
271
IND
*******
mo17
AAGTACC
b73


17
Amplicon50179
3279
265
272
IND
********
mo17
AAGTACCT
b73


17
Amplicon50179
3280
338
338
SNP
C
mo17
T
b73


18
Amplicon50182
3282
52
52
SNP
G
b73
T
mo17


18
Amplicon50182
3283
93
93
SNP
C
b73
G
mo17


18
Amplicon50182
3284
438
438
SNP
C
mo17
G
b73


18
Amplicon50182
3285
480
480
IND
*
mo17
G
b73


18
Amplicon50182
3286
537
539
IND
***
b73
CGA
mo17


18
Amplicon50182
3287
537
540
IND
****
b73
CGAT
mo17


19
Amplicon50190
3341
54
54
SNP
A
b73
G
mo17


19
Amplicon50190
3342
91
91
SNP
A
mo17
G
b73


19
Amplicon50190
3343
100
100
SNP
A
mo17
C
b73


19
Amplicon50190
3344
104
104
SNP
A
b73
T
mo17


19
Amplicon50190
3345
146
146
SNP
C
mo17
T
b73


19
Amplicon50190
3346
197
197
SNP
C
b73
G
mo17


19
Amplicon50190
3347
208
208
SNP
A
b73
G
mo17


19
Amplicon50190
3348
266
266
SNP
A
mo17
C
b73


19
Amplicon50190
3349
290
290
SNP
A
mo17
G
b73


19
Amplicon50190
3350
314
314
SNP
C
b73
T
mo17


19
Amplicon50190
3351
431
431
SNP
A
b73
C
mo17


19
Amplicon50190
3352
438
438
SNP
A
mo17
C
b73


19
Amplicon50190
3353
468
468
SNP
C
b73
T
mo17


19
Amplicon50190
3354
552
553
IND
**
b73
AA
mo17


20
Amplicon50302
3640
444
444
SNP
C
b73
G
mo17


21
Amplicon50453
3964
170
170
SNP
G
mo17
T
b73


21
Amplicon50453
3965
209
209
SNP
G
b73
T
mo17


21
Amplicon50453
3966
286
286
SNP
C
b73
T
mo17


21
Amplicon50453
3967
359
359
SNP
G
mo17
T
b73


22
Amplicon50455
3970
354
354
SNP
A
mo17
G
b73


23
Amplicon50517
4091
47
47
IND
*
b73
G
mo17


23
Amplicon50517
4092
51
51
SNP
C
b73
T
mo17


23
Amplicon50517
4093
67
67
SNP
A
b73
T
mo17


23
Amplicon50517
4094
74
74
IND
*
mo17
T
b73


23
Amplicon50517
4095
88
88
SNP
A
b73
C
mo17


23
Amplicon50517
4096
91
91
SNP
A
mo17
C
b73


23
Amplicon50517
4097
92
92
IND
*
b73
G
mo17


23
Amplicon50517
4098
146
146
SNP
A
b73
G
mo17


23
Amplicon50517
4099
181
185
IND
*****
b73
CCAGG
mo17


23
Amplicon50517
4100
231
231
SNP
A
mo17
G
b73


23
Amplicon50517
4101
290
293
IND
****
mo17
GATC
b73


23
Amplicon50517
4102
360
360
SNP
G
mo17
T
b73


23
Amplicon50517
4103
361
361
SNP
A
mo17
C
b73


23
Amplicon50517
4104
364
364
SNP
G
b73
T
mo17


23
Amplicon50517
4105
365
365
SNP
C
mo17
G
b73


23
Amplicon50517
4106
415
415
SNP
G
mo17
T
b73


23
Amplicon50517
4107
432
432
SNP
A
b73
C
mo17


24
Amplicon50606
4300
77
77
SNP
G
b73
T
mo17


24
Amplicon50606
4301
232
232
SNP
C
mo17
T
b73


24
Amplicon50606
4302
357
357
SNP
C
b73
T
mo17


25
Amplicon50664
4408
61
61
SNP
A
b73
G
mo17


25
Amplicon50664
4409
124
124
IND
*
mo17
A
b73


25
Amplicon50664
4410
203
203
SNP
C
mo17
T
b73


25
Amplicon50664
4411
207
207
SNP
A
mo17
G
b73


26
Amplicon50676
4415
291
291
SNP
A
mo17
C
b73


27
Amplicon50691
4444
26
26
SNP
A
b73
T
mo17


27
Amplicon50691
4445
273
273
SNP
C
mo17
T
b73


28
Amplicon50693
4446
33
33
SNP
C
b73
T
mo17


28
Amplicon50693
4447
41
41
SNP
A
mo17
G
b73


28
Amplicon50693
4448
68
68
SNP
C
mo17
G
b73


28
Amplicon50693
4449
111
111
SNP
C
mo17
G
b73


28
Amplicon50693
4450
124
124
SNP
A
b73
G
mo17


28
Amplicon50693
4451
328
328
SNP
A
b73
G
mo17


28
Amplicon50693
4452
340
340
SNP
A
mo17
T
b73


28
Amplicon50693
4453
377
377
SNP
C
b73
T
mo17


28
Amplicon50693
4454
412
412
SNP
A
b73
G
mo17


29
Amplicon50699
4463
281
281
SNP
C
b73
T
mo17


30
Amplicon50748
4598
100
100
SNP
A
mo17
C
b73


30
Amplicon50748
4599
104
104
SNP
C
mo17
T
b73


31
Amplicon50852
4886
548
548
SNP
C
b73
T
mo17


32
Amplicon50932
5021
371
371
SNP
C
mo17
T
b73


33
Amplicon50995
5097
109
109
IND
*
mo17
T
b73


33
Amplicon50995
5098
138
138
SNP
A
b73
G
mo17


33
Amplicon50995
5099
230
230
SNP
G
mo17
T
b73


33
Amplicon50995
5100
321
321
SNP
A
mo17
C
b73


33
Amplicon50995
5101
331
331
IND
*
b73
G
mo17


33
Amplicon50995
5102
351
351
SNP
A
b73
C
mo17


33
Amplicon50995
5103
366
366
SNP
A
mo17
G
b73


33
Amplicon50995
5104
417
417
SNP
A
b73
G
mo17


33
Amplicon50995
5105
436
436
SNP
A
b73
T
mo17


33
Amplicon50995
5106
519
519
SNP
C
b73
T
mo17


33
Amplicon50995
5107
576
576
SNP
A
b73
G
mo17


34
Amplicon51024
5139
189
189
SNP
C
mo17
T
b73


34
Amplicon51024
5140
347
347
SNP
C
mo17
T
b73


35
Amplicon51063
5215
494
494
SNP
A
mo17
T
b73


36
Amplicon51114
5274
185
185
IND
*
mo17
G
b73


36
Amplicon51114
5275
222
222
SNP
A
mo17
G
b73


36
Amplicon51114
5276
358
358
SNP
A
mo17
T
b73


36
Amplicon51114
5277
451
451
SNP
C
b73
T
mo17


37
Amplicon51134
5314
279
279
SNP
C
mo17
G
b73


37
Amplicon51134
5315
340
340
SNP
A
mo17
G
b73


37
Amplicon51134
5316
442
442
SNP
A
b73
G
mo17


37
Amplicon51134
5317
443
443
SNP
A
b73
C
mo17


37
Amplicon51134
5318
467
467
SNP
A
b73
T
mo17


37
Amplicon51134
5319
479
479
SNP
A
b73
G
mo17


38
Amplicon51241
5480
173
173
SNP
A
b73
G
mo17


38
Amplicon51241
5481
325
325
SNP
A
mo17
G
b73


38
Amplicon51241
5482
405
405
SNP
C
b73
T
mo17


39
Amplicon173892
8685
112
112
SNP
A
mo17
G
b73


40
Amplicon173943
8716
118
118
IND
*
b73
A
mo17


40
Amplicon173943
8717
129
129
SNP
C
mo17
G
b73


40
Amplicon173943
8718
197
197
SNP
A
mo17
G
b73


40
Amplicon173943
8719
257
257
SNP
A
b73
G
mo17


40
Amplicon173943
8720
286
286
SNP
A
b73
C
mo17


41
Amplicon173990
8757
285
285
SNP
C
mo17
T
b73


41
Amplicon173990
8758
323
323
SNP
A
b73
C
mo17


41
Amplicon173990
8759
379
379
SNP
A
b73
G
mo17


41
Amplicon173990
8760
412
412
SNP
A
b73
T
mo17


41
Amplicon173990
8761
413
413
IND
*
mo17
C
b73


41
Amplicon173990
8762
427
427
SNP
A
mo17
T
b73


42
Amplicon174021
8799
352
352
SNP
A
mo17
G
b73


42
Amplicon174021
8800
445
445
SNP
A
mo17
G
b73


43
Amplicon174108
8838
294
294
SNP
A
b73
G
mo17


44
Amplicon174113
8840
108
108
SNP
A
b73
G
mo17


45
Amplicon174252
8911
211
211
SNP
A
b73
G
mo17


46
Amplicon174322
8937
151
151
SNP
A
mo17
T
b73


47
Amplicon174423
8979
196
196
SNP
A
mo17
T
b73


48
Amplicon174434
8981
58
58
SNP
A
b73
G
mo17


48
Amplicon174434
8982
358
358
SNP
A
b73
G
mo17


49
Amplicon174442
8984
402
402
SNP
C
mo17
T
b73


50
Amplicon174632
9079
126
126
SNP
C
b73
T
mo17


51
Amplicon174763
9142
86
86
IND
*
b73
C
mo17


51
Amplicon174763
9143
96
96
IND
*
mo17
C
b73


51
Amplicon174763
9144
233
233
SNP
A
mo17
T
b73


52
Amplicon174793
9157
167
167
SNP
C
b73
G
mo17


52
Amplicon174793
9158
318
318
SNP
A
b73
G
mo17


52
Amplicon174793
9159
359
359
SNP
A
mo17
G
b73


52
Amplicon174793
9160
396
396
SNP
C
b73
T
mo17


52
Amplicon174793
9161
399
399
SNP
A
b73
G
mo17


52
Amplicon174793
9162
412
412
SNP
A
b73
C
mo17


53
Amplicon174811
9173
133
133
SNP
A
mo17
C
b73


53
Amplicon174811
9174
163
166
IND
****
mo17
GTAT
b73


53
Amplicon174811
9175
173
173
SNP
A
b73
G
mo17


53
Amplicon174811
9176
174
174
SNP
C
mo17
T
b73


54
Amplicon174832
9187
146
146
SNP
C
b73
T
mo17


54
Amplicon174832
9188
244
245
IND
**
b73
TT
mo17


54
Amplicon174832
9189
248
249
IND
**
b73
CC
mo17


54
Amplicon174832
9190
268
268
SNP
C
mo17
T
b73


54
Amplicon174832
9191
275
275
IND
*
b73
T
mo17


54
Amplicon174832
9192
285
285
SNP
A
mo17
G
b73


54
Amplicon174832
9193
367
367
SNP
C
mo17
G
b73


55
Amplicon175020
9297
118
118
SNP
A
mo17
G
b73


55
Amplicon175020
9298
189
189
SNP
A
mo17
T
b73


55
Amplicon175020
9299
195
195
SNP
A
b73
G
mo17


56
Amplicon175137
9364
409
409
SNP
C
mo17
T
b73


57
Amplicon175303
9449
209
209
SNP
A
mo17
G
b73


57
Amplicon175303
9450
262
266
IND
*****
mo17
CCTTG
b73


57
Amplicon175303
9451
426
426
SNP
A
mo17
T
b73


58
Amplicon175327
9467
57
57
IND
*
mo17
T
b73


58
Amplicon175327
9468
126
126
SNP
A
b73
G
mo17


58
Amplicon175327
9469
333
333
SNP
A
b73
G
mo17


59
Amplicon175328
9470
154
154
SNP
C
mo17
G
b73


59
Amplicon175328
9471
213
215
IND
***
b73
TAG
mo17


60
Amplicon175332
9472
146
146
IND
*
b73
A
mo17


60
Amplicon175332
9473
335
335
SNP
C
mo17
T
b73


61
Amplicon175347
9485
153
153
SNP
A
b73
C
mo17


61
Amplicon175347
9486
242
242
SNP
A
b73
T
mo17


62
Amplicon175497
9553
73
73
SNP
A
b73
T
mo17


62
Amplicon175497
9554
263
263
SNP
A
mo17
T
b73


62
Amplicon175497
9555
284
284
SNP
A
mo17
G
b73


62
Amplicon175497
9556
289
289
SNP
A
mo17
C
b73


62
Amplicon175497
9557
374
374
SNP
A
mo17
C
b73


63
Amplicon175582
9622
37
37
SNP
C
mo17
T
b73


63
Amplicon175582
9623
198
198
SNP
C
b73
T
mo17


63
Amplicon175582
9624
258
258
SNP
A
b73
G
mo17


63
Amplicon175582
9625
354
354
SNP
C
mo17
T
b73


64
Amplicon175589
9626
238
238
SNP
C
mo17
G
b73


64
Amplicon175589
9627
260
260
SNP
A
b73
C
mo17


65
Amplicon175642
9667
240
240
SNP
C
b73
G
mo17


66
Amplicon175645
9668
108
108
SNP
A
b73
G
mo17


66
Amplicon175645
9669
388
388
SNP
A
mo17
T
b73


67
Amplicon175693
9698
330
330
SNP
C
b73
G
mo17


67
Amplicon175693
9699
347
350
IND
****
b73
GTTG
mo17


67
Amplicon175693
9700
352
354
IND
***
b73
TTT
mo17


67
Amplicon175693
9701
448
448
SNP
A
b73
G
mo17


68
Amplicon175701
9703
21
21
SNP
G
b73
T
mo17


68
Amplicon175701
9704
61
61
SNP
C
mo17
T
b73


68
Amplicon175701
9705
213
213
SNP
C
b73
T
mo17


68
Amplicon175701
9706
284
284
SNP
A
mo17
G
b73


68
Amplicon175701
9707
381
381
SNP
A
mo17
G
b73


68
Amplicon175701
9708
402
402
SNP
G
b73
T
mo17


68
Amplicon175701
9709
418
418
SNP
C
b73
T
mo17


68
Amplicon175701
9710
419
419
SNP
A
mo17
C
b73


68
Amplicon175701
9711
435
435
SNP
A
mo17
G
b73


69
Amplicon175758
9739
290
290
SNP
A
b73
G
mo17


70
Amplicon175783
9759
167
167
SNP
A
mo17
G
b73


70
Amplicon175783
9760
220
220
SNP
A
b73
G
mo17


70
Amplicon175783
9761
275
275
SNP
A
mo17
G
b73


70
Amplicon175783
9762
282
282
SNP
A
b73
T
mo17


70
Amplicon175783
9763
327
330
IND
****
mo17
ACGA
b73


70
Amplicon175783
9764
476
476
SNP
A
mo17
G
b73


70
Amplicon175783
9765
483
483
IND
*
mo17
C
b73


71
Amplicon176287
9864
36
36
SNP
C
mo17
T
b73


71
Amplicon176287
9865
155
155
SNP
A
b73
C
mo17


71
Amplicon176287
9866
159
159
SNP
A
mo17
T
b73


71
Amplicon176287
9867
247
247
SNP
A
b73
T
mo17


72
Amplicon176352
9927
40
40
SNP
A
mo17
T
b73


72
Amplicon176352
10667
308
308
SNP
A
mo17
G
b73


73
Amplicon176515
10323
100
100
SNP
A
mo17
G
b73


73
Amplicon176515
10324
105
105
SNP
A
b73
G
mo17


73
Amplicon176515
10325
111
111
SNP
C
b73
G
mo17


73
Amplicon176515
10326
112
112
SNP
C
mo17
T
b73


73
Amplicon176515
10327
117
117
IND
*
mo17
C
b73


73
Amplicon176515
10328
119
120
IND
**
mo17
TC
b73


73
Amplicon176515
10329
143
143
SNP
C
mo17
T
b73


73
Amplicon176515
10330
157
157
SNP
C
b73
G
mo17


73
Amplicon176515
10331
160
160
IND
*
b73
C
mo17


73
Amplicon176515
10332
174
175
IND
**
mo17
TA
b73


73
Amplicon176515
10334
252
252
IND
*
mo17
C
b73


73
Amplicon176515
10335
269
269
IND
*
b73
C
mo17


73
Amplicon176515
10336
269
270
IND
**
b73
CA
mo17


73
Amplicon176515
10337
417
422
IND
******
mo17
CAGAGA
b73


73
Amplicon176515
10338
462
462
SNP
A
b73
G
mo17


73
Amplicon176515
11466
213
213
SNP
G
b73
T
mo17


73
Amplicon176515
11472
320
320
SNP
A
b73
G
mo17


74
Amplicon176522
11522
249
249
SNP
A
b73
C
mo17


75
Amplicon176583
10618
162
162
SNP
C
mo17
T
b73


75
Amplicon176583
12012
128
128
SNP
C
b73
T
mo17


76
Amplicon176608
10668
27
27
SNP
A
b73
C
mo17


76
Amplicon176608
10669
46
46
SNP
A
b73
G
mo17


76
Amplicon176608
10671
71
71
SNP
A
mo17
C
b73


76
Amplicon176608
10673
227
227
SNP
A
mo17
C
b73


76
Amplicon176608
10674
246
246
SNP
C
b73
T
mo17


76
Amplicon176608
10675
283
283
IND
*
b73
T
mo17


76
Amplicon176608
10677
340
340
SNP
A
b73
G
mo17


77
Amplicon176683
12340
401
401
SNP
A
mo17
T
b73


78
Amplicon176749
11302
77
77
SNP
A
b73
C
mo17


78
Amplicon176749
11304
227
227
SNP
C
b73
G
mo17


78
Amplicon176749
11306
284
284
SNP
A
mo17
G
b73


78
Amplicon176749
11308
286
286
SNP
C
mo17
T
b73


78
Amplicon176749
11310
373
373
SNP
A
mo17
C
b73


78
Amplicon176749
11311
374
374
IND
*
mo17
C
b73


78
Amplicon176749
11313
393
393
SNP
A
mo17
G
b73


78
Amplicon176749
11316
538
538
SNP
A
b73
G
mo17


78
Amplicon176749
11318
609
609
SNP
A
mo17
G
b73


78
Amplicon176749
12711
512
512
SNP
A
mo17
G
b73


79
Amplicon176767
12824
411
411
SNP
C
b73
T
mo17


80
Amplicon176822
11713
300
300
SNP
C
mo17
G
b73


80
Amplicon176822
13100
286
286
SNP
A
b73
C
mo17


81
Amplicon176863
12092
241
241
SNP
C
mo17
T
b73


81
Amplicon176863
12093
297
298
IND
**
mo17
TT
b73


81
Amplicon176863
12094
322
322
SNP
A
b73
G
mo17


81
Amplicon176863
12096
345
345
SNP
A
b73
G
mo17


81
Amplicon176863
12098
422
422
SNP
C
b73
T
mo17


81
Amplicon176863
13490
481
481
SNP
C
b73
T
mo17


82
Amplicon176899
12427
28
28
SNP
C
mo17
T
b73


82
Amplicon176899
12428
136
136
SNP
G
b73
T
mo17


82
Amplicon176899
12430
205
205
SNP
A
mo17
C
b73


82
Amplicon176899
12432
237
237
SNP
C
mo17
T
b73


82
Amplicon176899
12434
405
405
SNP
A
b73
G
mo17


82
Amplicon176899
13833
440
440
SNP
A
mo17
G
b73


83
Amplicon176937
12557
134
134
SNP
A
b73
G
mo17


83
Amplicon176937
12559
305
305
SNP
A
mo17
G
b73


83
Amplicon176937
12561
368
368
SNP
A
mo17
G
b73


83
Amplicon176937
12563
382
382
IND
*
b73
C
mo17


83
Amplicon176937
12565
384
384
IND
*
b73
C
mo17


83
Amplicon176937
12567
386
386
IND
*
b73
C
mo17


83
Amplicon176937
12569
388
388
IND
*
b73
C
mo17


83
Amplicon176937
12571
391
391
SNP
A
mo17
G
b73


83
Amplicon176937
12573
392
392
SNP
A
mo17
T
b73


83
Amplicon176937
12575
393
393
SNP
G
mo17
T
b73


84
Amplicon177031
13082
460
461
IND
**
b73
TA
mo17


84
Amplicon177031
14467
370
371
IND
**
b73
AT
mo17


85
Amplicon177035
13083
118
118
SNP
A
mo17
G
b73


85
Amplicon177035
13086
364
364
SNP
A
mo17
G
b73


85
Amplicon177035
13088
384
384
SNP
A
b73
G
mo17


86
Amplicon177038
14479
308
308
SNP
G
mo17
T
b73


87
Amplicon177055
13160
585
585
SNP
C
b73
T
mo17


87
Amplicon177055
14545
513
513
SNP
G
b73
T
mo17


88
Amplicon177093
13283
139
139
SNP
A
b73
T
mo17


88
Amplicon177093
14666
125
125
SNP
C
mo17
G
b73


89
Amplicon177147
13685
230
230
SNP
A
b73
G
mo17


89
Amplicon177147
13687
245
245
SNP
C
b73
T
mo17


89
Amplicon177147
13688
300
300
SNP
A
b73
C
mo17


89
Amplicon177147
13689
392
392
SNP
A
b73
C
mo17


89
Amplicon177147
13691
489
489
SNP
C
mo17
T
b73


90
Amplicon177150
13714
204
204
SNP
A
mo17
G
b73


90
Amplicon177150
13716
224
224
SNP
C
b73
G
mo17


90
Amplicon177150
13718
283
283
SNP
C
b73
T
mo17


90
Amplicon177150
13720
424
424
SNP
C
mo17
T
b73


90
Amplicon177150
13722
450
450
SNP
A
b73
G
mo17


90
Amplicon177150
15096
397
397
SNP
A
b73
G
mo17


91
Amplicon177154
13745
51
51
SNP
A
b73
G
mo17


91
Amplicon177154
13747
71
71
SNP
C
b73
T
mo17


91
Amplicon177154
13748
114
114
SNP
C
mo17
T
b73


91
Amplicon177154
13750
152
152
SNP
A
b73
T
mo17


91
Amplicon177154
13752
312
312
SNP
A
mo17
G
b73


91
Amplicon177154
13754
449
449
SNP
C
b73
T
mo17


91
Amplicon177154
13756
498
498
SNP
A
b73
G
mo17


92
Amplicon177165
13783
66
66
SNP
A
b73
G
mo17


92
Amplicon177165
13785
101
101
SNP
C
mo17
T
b73


92
Amplicon177165
13787
111
111
IND
*
mo17
T
b73


92
Amplicon177165
13791
143
143
SNP
C
mo17
T
b73


92
Amplicon177165
13793
144
144
SNP
A
mo17
T
b73


92
Amplicon177165
13795
190
190
SNP
A
mo17
T
b73


92
Amplicon177165
13797
191
191
SNP
A
b73
C
mo17


92
Amplicon177165
13799
193
193
SNP
C
mo17
G
b73


92
Amplicon177165
13801
229
229
SNP
A
b73
G
mo17


92
Amplicon177165
13803
241
243
IND
***
b73
TAC
mo17


92
Amplicon177165
13805
274
274
SNP
A
b73
G
mo17


92
Amplicon177165
13807
334
334
SNP
A
mo17
C
b73


92
Amplicon177165
13811
567
567
SNP
C
b73
T
mo17


92
Amplicon177165
15184
390
390
SNP
C
b73
T
mo17


93
Amplicon177340
14596
216
216
SNP
C
b73
T
mo17


93
Amplicon177340
14599
502
502
SNP
A
mo17
C
b73


93
Amplicon177340
15954
253
253
SNP
A
b73
T
mo17


94
Amplicon177341
14602
67
67
SNP
A
b73
G
mo17


94
Amplicon177341
14604
102
102
SNP
A
b73
T
mo17


94
Amplicon177341
14606
187
187
SNP
C
b73
T
mo17


94
Amplicon177341
15965
389
389
SNP
A
b73
T
mo17


95
Amplicon177359
16041
189
189
SNP
A
mo17
C
b73


96
Amplicon177361
14692
74
74
SNP
C
b73
G
mo17


96
Amplicon177361
14694
104
104
SNP
A
mo17
C
b73


96
Amplicon177361
14697
528
528
SNP
C
b73
T
mo17


96
Amplicon177361
14698
556
556
SNP
C
b73
T
mo17


96
Amplicon177361
14700
560
560
SNP
G
mo17
T
b73


97
Amplicon177370
16074
384
384
SNP
A
b73
G
mo17


98
Amplicon177382
16137
481
481
SNP
C
b73
T
mo17


99
Amplicon177440
15165
229
229
SNP
A
mo17
G
b73


99
Amplicon177440
15167
241
241
SNP
C
b73
T
mo17


99
Amplicon177440
15171
411
411
SNP
A
mo17
T
b73


99
Amplicon177440
15173
412
412
IND
*
b73
A
mo17


99
Amplicon177440
15175
433
433
SNP
A
b73
G
mo17


99
Amplicon177440
15177
501
501
SNP
A
b73
G
mo17


99
Amplicon177440
16527
353
353
SNP
A
b73
T
mo17


100
Amplicon177450
15244
344
345
IND
**
b73
AT
mo17


100
Amplicon177450
15245
475
475
IND
*
b73
T
mo17


100
Amplicon177450
15247
482
482
SNP
C
mo17
T
b73


101
Amplicon177463
15304
79
79
SNP
A
mo17
G
b73


101
Amplicon177463
15305
326
326
SNP
A
mo17
G
b73


102
Amplicon177482
15369
21
21
SNP
G
mo17
T
b73


102
Amplicon177482
15373
256
256
SNP
A
b73
G
mo17


102
Amplicon177482
16724
87
87
SNP
C
mo17
T
b73


103
Amplicon177485
15378
447
447
IND
*
b73
T
mo17


103
Amplicon177485
15379
455
455
SNP
G
mo17
T
b73


103
Amplicon177485
15381
465
465
SNP
C
b73
G
mo17


103
Amplicon177485
15383
503
503
SNP
A
b73
C
mo17


103
Amplicon177485
16729
103
103
SNP
C
b73
T
mo17


104
Amplicon177492
15405
81
84
IND
****
mo17
CATG
b73


104
Amplicon177492
15407
136
136
SNP
C
b73
G
mo17


104
Amplicon177492
15409
194
194
SNP
A
b73
G
mo17


104
Amplicon177492
15411
275
275
SNP
C
b73
G
mo17


104
Amplicon177492
15415
404
404
SNP
C
mo17
T
b73


104
Amplicon177492
15417
532
532
SNP
C
mo17
G
b73


104
Amplicon177492
16762
379
379
SNP
A
mo17
C
b73


105
Amplicon177493
16767
61
61
SNP
A
mo17
C
b73


106
Amplicon177524
15604
141
141
SNP
A
mo17
G
b73


106
Amplicon177524
16944
231
231
SNP
A
mo17
G
b73


107
Amplicon177615
16016
112
112
SNP
C
b73
T
mo17


107
Amplicon177615
16017
158
158
SNP
A
mo17
T
b73


107
Amplicon177615
16019
286
286
SNP
A
b73
G
mo17


107
Amplicon177615
16021
288
288
SNP
A
mo17
G
b73


107
Amplicon177615
16023
295
295
SNP
A
b73
G
mo17


107
Amplicon177615
16024
334
334
SNP
C
b73
T
mo17


107
Amplicon177615
16026
381
381
SNP
A
b73
G
mo17


108
Amplicon177707
16497
125
125
SNP
A
b73
G
mo17


108
Amplicon177707
17828
340
340
SNP
A
mo17
G
b73


109
Amplicon177729
16576
63
63
SNP
C
mo17
T
b73


109
Amplicon177729
16578
83
83
SNP
A
mo17
T
b73


109
Amplicon177729
16582
208
208
SNP
G
mo17
T
b73


109
Amplicon177729
16584
248
248
SNP
C
mo17
T
b73


109
Amplicon177729
16585
250
253
IND
****
b73
GGAC
mo17


109
Amplicon177729
16588
331
331
SNP
G
mo17
T
b73


109
Amplicon177729
16589
377
377
SNP
G
mo17
T
b73


109
Amplicon177729
16591
391
391
SNP
A
b73
T
mo17


109
Amplicon177729
16593
397
397
SNP
C
b73
T
mo17


109
Amplicon177729
16595
398
398
IND
*
b73
T
mo17


109
Amplicon177729
17900
155
155
SNP
A
mo17
G
b73


109
Amplicon177729
17908
256
259
IND
****
b73
CTGG
mo17


110
Amplicon177761
16726
150
150
SNP
A
b73
G
mo17


110
Amplicon177761
16727
152
152
SNP
A
mo17
G
b73


110
Amplicon177761
16728
184
184
SNP
C
b73
T
mo17


110
Amplicon177761
16730
302
302
SNP
A
mo17
G
b73


111
Amplicon177766
16752
26
26
SNP
C
mo17
T
b73


111
Amplicon177766
16753
59
59
SNP
C
mo17
G
b73


111
Amplicon177766
16755
140
140
SNP
C
mo17
T
b73


111
Amplicon177766
16757
229
229
SNP
C
b73
T
mo17


111
Amplicon177766
16759
239
239
SNP
C
mo17
G
b73


111
Amplicon177766
16761
242
242
SNP
A
mo17
G
b73


111
Amplicon177766
16763
265
265
SNP
G
b73
T
mo17


112
Amplicon177785
16839
146
146
SNP
A
mo17
C
b73


112
Amplicon177785
18157
576
576
SNP
C
b73
T
mo17


113
Amplicon177791
16876
90
90
SNP
A
mo17
G
b73


113
Amplicon177791
16877
108
112
IND
*****
mo17
AGTAC
b73


113
Amplicon177791
16878
197
197
SNP
C
b73
G
mo17


113
Amplicon177791
16880
199
200
IND
**
b73
CA
mo17


113
Amplicon177791
16882
202
202
SNP
G
mo17
T
b73


113
Amplicon177791
16883
205
205
SNP
A
b73
G
mo17


113
Amplicon177791
16885
206
206
SNP
C
b73
G
mo17


113
Amplicon177791
16887
209
210
IND
**
b73
CG
mo17


113
Amplicon177791
16889
214
215
IND
**
b73
AA
mo17


113
Amplicon177791
16891
218
218
IND
*
b73
A
mo17


113
Amplicon177791
16893
220
220
IND
*
b73
T
mo17


113
Amplicon177791
16895
259
259
SNP
A
mo17
C
b73


114
Amplicon177843
17079
516
516
SNP
C
b73
G
mo17


114
Amplicon177843
18392
356
356
SNP
C
b73
T
mo17


115
Amplicon177848
17120
150
150
SNP
A
mo17
G
b73


115
Amplicon177848
18439
171
171
SNP
A
b73
G
mo17


116
Amplicon178002
17897
336
336
SNP
A
b73
G
mo17


116
Amplicon178002
19198
322
322
SNP
C
mo17
T
b73


117
Amplicon178141
18677
919
919
SNP
C
mo17
T
b73


117
Amplicon178141
18679
921
921
SNP
C
b73
T
mo17


117
Amplicon178141
18681
1033
1033
SNP
C
mo17
T
b73


117
Amplicon178141
18683
1087
1087
SNP
A
b73
G
mo17


117
Amplicon178141
18685
1099
1099
SNP
A
mo17
T
b73


117
Amplicon178141
18689
1223
1223
SNP
C
b73
T
mo17


117
Amplicon178141
19963
1174
1174
SNP
A
b73
C
mo17


118
Amplicon178159
20048
415
415
SNP
A
b73
G
mo17


119
Amplicon178261
19236
152
152
SNP
A
mo17
G
b73


119
Amplicon178261
19240
243
243
SNP
A
mo17
G
b73


119
Amplicon178261
19241
245
245
SNP
G
mo17
T
b73


119
Amplicon178261
19242
406
406
SNP
C
mo17
T
b73


119
Amplicon178261
20502
171
171
SNP
A
b73
G
mo17


120
Amplicon178278
19266
113
113
SNP
G
b73
T
mo17


120
Amplicon178278
19268
114
116
IND
***
mo17
GAG
b73


120
Amplicon178278
19271
158
158
SNP
A
mo17
G
b73


120
Amplicon178278
20537
139
139
SNP
C
b73
T
mo17


121
Amplicon178389
19661
48
48
SNP
G
mo17
T
b73


121
Amplicon178389
19663
338
338
SNP
C
mo17
T
b73


121
Amplicon178389
20933
372
372
SNP
C
mo17
T
b73


122
Amplicon178390
19669
299
299
SNP
A
b73
C
mo17


122
Amplicon178390
20934
172
172
SNP
A
mo17
G
b73


123
Amplicon178392
19702
81
81
SNP
G
mo17
T
b73


123
Amplicon178392
19704
182
182
SNP
A
b73
T
mo17


123
Amplicon178392
19705
402
402
IND
*
b73
A
mo17


124
Amplicon178401
19767
38
38
SNP
G
b73
T
mo17


124
Amplicon178401
19771
178
178
SNP
G
mo17
T
b73


124
Amplicon178401
19773
193
193
SNP
A
mo17
C
b73


124
Amplicon178401
19775
205
210
IND
******
b73
GGAGAG
mo17


124
Amplicon178401
19777
219
223
IND
*****
b73
AAAAA
mo17


124
Amplicon178401
19782
420
420
SNP
C
mo17
T
b73


124
Amplicon178401
19784
428
428
SNP
A
b73
G
mo17


124
Amplicon178401
21038
125
125
SNP
A
mo17
T
b73


124
Amplicon178401
21045
307
307
SNP
C
b73
T
mo17


125
Amplicon178430
19893
113
113
SNP
G
mo17
T
b73


125
Amplicon178430
19896
239
239
SNP
A
mo17
C
b73


125
Amplicon178430
19897
465
465
SNP
C
b73
T
mo17


125
Amplicon178430
21154
54
54
SNP
C
b73
T
mo17


126
Amplicon178454
20172
157
157
SNP
C
mo17
T
b73


126
Amplicon178454
20173
172
172
SNP
C
b73
G
mo17


126
Amplicon178454
20175
194
194
SNP
G
b73
T
mo17


126
Amplicon178454
20177
203
203
SNP
A
mo17
C
b73


126
Amplicon178454
20180
296
296
SNP
A
b73
C
mo17


126
Amplicon178454
20182
297
297
SNP
C
b73
T
mo17


126
Amplicon178454
20184
335
335
SNP
A
b73
C
mo17


126
Amplicon178454
20187
367
367
SNP
G
b73
T
mo17


126
Amplicon178454
20189
371
371
SNP
A
mo17
G
b73


126
Amplicon178454
20191
402
402
SNP
A
mo17
C
b73


126
Amplicon178454
21433
59
59
SNP
C
mo17
G
b73


126
Amplicon178454
21443
223
223
SNP
A
b73
G
mo17


127
Amplicon178494
20348
303
303
SNP
C
b73
T
mo17


127
Amplicon178494
20350
391
391
SNP
A
b73
T
mo17


127
Amplicon178494
21603
180
180
SNP
G
b73
T
mo17


128
Amplicon178507
20406
52
52
SNP
C
mo17
G
b73


128
Amplicon178507
20408
219
219
SNP
G
mo17
T
b73


128
Amplicon178507
20410
283
283
SNP
A
b73
T
mo17


128
Amplicon178507
20412
294
294
SNP
G
mo17
T
b73


128
Amplicon178507
20414
359
359
SNP
A
mo17
G
b73


128
Amplicon178507
20416
396
396
SNP
A
mo17
G
b73


128
Amplicon178507
20418
397
397
SNP
G
mo17
T
b73


128
Amplicon178507
20420
429
429
SNP
C
b73
T
mo17


128
Amplicon178507
20422
432
432
SNP
C
b73
G
mo17


128
Amplicon178507
20424
457
457
SNP
C
b73
T
mo17


128
Amplicon178507
20426
462
462
SNP
G
mo17
T
b73


129
Amplicon178546
20513
147
147
SNP
A
b73
C
mo17


129
Amplicon178546
20515
180
180
SNP
C
b73
T
mo17


129
Amplicon178546
20517
312
312
SNP
C
mo17
T
b73


129
Amplicon178546
20519
336
336
SNP
A
b73
C
mo17


129
Amplicon178546
20521
342
342
SNP
C
b73
T
mo17


129
Amplicon178546
20523
396
396
SNP
C
b73
T
mo17


129
Amplicon178546
20525
435
435
SNP
C
mo17
T
b73


129
Amplicon178546
21772
258
258
SNP
C
b73
T
mo17


130
Amplicon178569
21895
37
37
SNP
C
b73
T
mo17


131
Amplicon178666
21190
285
285
SNP
A
b73
G
mo17


131
Amplicon178666
21192
498
498
SNP
C
mo17
T
b73


132
Amplicon178673
21292
130
130
SNP
C
mo17
T
b73


133
Amplicon178674
22541
130
130
SNP
C
mo17
T
b73


134
Amplicon178700
22717
63
63
SNP
A
mo17
T
b73


135
Amplicon178723
21524
115
115
IND
*
mo17
T
b73


135
Amplicon178723
21526
117
117
IND
*
mo17
A
b73


135
Amplicon178723
21528
209
215
IND
*******
b73
AGCTAGC
mo17


135
Amplicon178723
21530
217
217
IND
*
b73
T
mo17


135
Amplicon178723
21532
481
481
SNP
C
mo17
T
b73


135
Amplicon178723
21533
485
485
SNP
C
mo17
G
b73


135
Amplicon178723
21535
487
487
SNP
C
b73
G
mo17


135
Amplicon178723
21536
488
488
IND
*
mo17
T
b73


135
Amplicon178723
21539
490
490
SNP
C
b73
T
mo17


135
Amplicon178723
21541
496
496
SNP
A
mo17
T
b73


135
Amplicon178723
21543
500
501
IND
**
mo17
GC
b73


135
Amplicon178723
21545
503
503
SNP
A
b73
G
mo17


135
Amplicon178723
22775
526
526
SNP
A
mo17
G
b73


136
Amplicon178726
21564
163
163
SNP
C
mo17
T
b73


136
Amplicon178726
22796
198
198
SNP
A
mo17
G
b73


137
Amplicon178785
23091
169
169
SNP
G
b73
T
mo17


138
Amplicon178833
23289
250
250
SNP
A
b73
G
mo17


139
Amplicon178895
23779
342
342
SNP
A
mo17
G
b73


140
Amplicon179006
23075
54
54
SNP
A
b73
T
mo17


140
Amplicon179006
23078
224
224
SNP
A
mo17
G
b73


140
Amplicon179006
23080
306
306
SNP
A
b73
G
mo17


140
Amplicon179006
23082
329
329
IND
*
mo17
C
b73


140
Amplicon179006
23085
342
342
SNP
A
b73
G
mo17


140
Amplicon179006
23086
363
363
SNP
C
mo17
T
b73


140
Amplicon179006
23087
390
390
SNP
A
b73
C
mo17


140
Amplicon179006
23089
503
503
SNP
A
b73
T
mo17


140
Amplicon179006
24265
136
136
SNP
A
b73
G
mo17


141
Amplicon179027
23203
38
40
IND
***
mo17
TCA
b73


141
Amplicon179027
23207
282
282
SNP
C
b73
G
mo17


141
Amplicon179027
24395
76
76
SNP
C
b73
T
mo17


142
Amplicon179035
24422
45
45
SNP
C
mo17
T
b73


143
Amplicon179070
23438
32
32
SNP
A
mo17
C
b73


143
Amplicon179070
23440
71
71
SNP
A
b73
T
mo17


143
Amplicon179070
23442
353
353
SNP
C
mo17
G
b73


144
Amplicon179140
23820
41
41
SNP
C
mo17
G
b73


144
Amplicon179140
23822
50
50
IND
*
b73
G
mo17


144
Amplicon179140
25000
72
72
SNP
A
mo17
G
b73


145
Amplicon179151
23890
120
120
SNP
C
b73
T
mo17


145
Amplicon179151
23892
476
476
SNP
C
mo17
T
b73


146
Amplicon179283
24647
190
190
SNP
A
b73
G
mo17


146
Amplicon179283
24649
265
265
SNP
A
mo17
G
b73


146
Amplicon179283
24651
375
375
SNP
C
b73
T
mo17


146
Amplicon179283
24653
431
431
SNP
A
b73
G
mo17


147
Amplicon179301
24693
92
92
SNP
A
b73
G
mo17


147
Amplicon179301
24697
201
201
SNP
C
b73
T
mo17


147
Amplicon179301
24699
245
245
SNP
C
b73
T
mo17


147
Amplicon179301
24701
272
272
SNP
C
b73
T
mo17


147
Amplicon179301
24703
395
395
SNP
A
b73
G
mo17


147
Amplicon179301
24704
418
418
SNP
C
mo17
T
b73


147
Amplicon179301
24705
505
505
SNP
C
mo17
T
b73


147
Amplicon179301
25863
128
128
SNP
A
mo17
G
b73


148
Amplicon179323
25961
399
399
SNP
A
b73
G
mo17


149
Amplicon179392
25414
23
23
SNP
A
mo17
G
b73


149
Amplicon179392
25416
212
212
SNP
A
mo17
G
b73


149
Amplicon179392
25418
224
226
IND
***
mo17
ATC
b73


150
Amplicon179414
26846
467
467
SNP
A
b73
C
mo17


151
Amplicon179487
26151
183
183
SNP
C
mo17
T
b73


151
Amplicon179487
26153
207
209
IND
***
mo17
CGG
b73


151
Amplicon179487
26155
212
213
IND
**
mo17
AC
b73


151
Amplicon179487
26157
215
216
IND
**
mo17
CC
b73


151
Amplicon179487
26159
236
236
IND
*
mo17
G
b73


151
Amplicon179487
26163
306
306
SNP
C
b73
T
mo17


151
Amplicon179487
26165
339
339
SNP
A
b73
T
mo17


151
Amplicon179487
26167
407
407
SNP
A
mo17
C
b73


151
Amplicon179487
26169
408
408
SNP
A
mo17
C
b73


151
Amplicon179487
26171
409
409
SNP
A
b73
G
mo17


151
Amplicon179487
27295
279
279
SNP
A
mo17
G
b73


152
Amplicon179497
26233
118
118
SNP
C
mo17
G
b73


152
Amplicon179497
26235
167
167
SNP
A
b73
G
mo17


152
Amplicon179497
26237
178
178
SNP
A
mo17
G
b73


152
Amplicon179497
26239
287
287
SNP
A
b73
G
mo17


152
Amplicon179497
26241
370
370
SNP
G
b73
T
mo17


152
Amplicon179497
26243
423
423
SNP
G
mo17
T
b73


152
Amplicon179497
26245
433
433
SNP
A
mo17
G
b73


152
Amplicon179497
26247
462
462
SNP
C
mo17
T
b73


152
Amplicon179497
27375
478
478
SNP
A
b73
C
mo17


153
Amplicon179515
26314
16
16
SNP
A
b73
G
mo17


153
Amplicon179515
26316
33
33
IND
*
b73
A
mo17


153
Amplicon179515
26318
95
95
SNP
A
b73
G
mo17


153
Amplicon179515
26319
132
132
SNP
A
b73
G
mo17


153
Amplicon179515
26321
161
161
SNP
C
mo17
G
b73


153
Amplicon179515
26322
281
283
IND
***
b73
CTG
mo17


153
Amplicon179515
26326
351
351
SNP
A
mo17
C
b73


153
Amplicon179515
27447
310
310
SNP
C
b73
G
mo17


154
Amplicon179595
26711
30
30
SNP
C
mo17
T
b73


154
Amplicon179595
26713
51
51
SNP
A
mo17
T
b73


154
Amplicon179595
26715
155
155
IND
*
mo17
C
b73


154
Amplicon179595
26717
187
187
SNP
C
mo17
T
b73


154
Amplicon179595
26719
194
194
SNP
C
b73
G
mo17


154
Amplicon179595
26720
213
213
SNP
A
b73
C
mo17


154
Amplicon179595
26722
233
233
SNP
C
b73
T
mo17


154
Amplicon179595
26723
285
285
SNP
C
mo17
T
b73


154
Amplicon179595
26725
395
395
SNP
G
mo17
T
b73


155
Amplicon179720
27268
249
249
SNP
A
b73
G
mo17


155
Amplicon179720
27272
336
336
SNP
A
b73
T
mo17


155
Amplicon179720
27274
355
355
SNP
A
b73
G
mo17


155
Amplicon179720
27276
357
357
IND
*
mo17
C
b73


155
Amplicon179720
27278
368
368
SNP
C
b73
G
mo17


155
Amplicon179720
28164
267
267
SNP
C
mo17
G
b73


156
Amplicon179741
27359
20
20
SNP
A
mo17
G
b73


156
Amplicon179741
27360
218
218
SNP
C
mo17
G
b73


156
Amplicon179741
27361
228
228
SNP
C
mo17
T
b73


156
Amplicon179741
27363
459
459
SNP
C
mo17
T
b73


157
Amplicon179806
27613
32
32
SNP
A
b73
G
mo17


157
Amplicon179806
27615
235
235
IND
*
mo17
T
b73


158
Amplicon179868
27874
345
345
SNP
C
b73
T
mo17


159
Amplicon234986
28913
103
103
IND
*
b73
C
mo17


159
Amplicon234986
28914
149
149
IND
*
mo17
C
b73


159
Amplicon234986
28915
149
150
IND
**
mo17
C*
b73


159
Amplicon234986
28916
169
169
SNP
A
b73
G
mo17


159
Amplicon234986
28917
256
256
SNP
A
b73
C
mo17


159
Amplicon234986
28918
311
311
SNP
C
b73
G
mo17


159
Amplicon234986
28919
469
469
SNP
G
b73
T
mo17


159
Amplicon234986
28920
501
501
SNP
A
mo17
G
b73


159
Amplicon234986
28921
834
834
SNP
A
b73
T
mo17


159
Amplicon234986
28922
911
911
SNP
A
mo17
T
b73


159
Amplicon234986
28923
1056
1056
SNP
C
b73
G
mo17


160
Amplicon234993
28931
175
175
IND
*
mo17
A
b73


160
Amplicon234993
28932
1103
1103
SNP
A
mo17
G
b73


161
Amplicon234996
28933
354
354
SNP
A
mo17
C
b73


161
Amplicon234996
28934
555
555
SNP
C
mo17
T
b73


161
Amplicon234996
28935
593
593
SNP
A
mo17
G
b73


162
Amplicon235018
29005
243
243
SNP
A
b73
G
mo17


163
Amplicon235123
29193
287
287
IND
*
mo17
C
b73


163
Amplicon235123
29194
291
291
SNP
A
b73
G
mo17


163
Amplicon235123
29195
673
678
IND
******
mo17
GCATTA
b73


163
Amplicon235123
29196
757
757
SNP
A
b73
G
mo17


164
Amplicon235210
29362
105
105
SNP
C
b73
T
mo17


164
Amplicon235210
29363
135
135
SNP
A
b73
G
mo17


164
Amplicon235210
29364
145
145
SNP
C
b73
G
mo17


164
Amplicon235210
29365
168
168
SNP
A
mo17
G
b73


164
Amplicon235210
29366
207
207
IND
*
mo17
C
b73


164
Amplicon235210
29367
218
218
SNP
A
b73
C
mo17


164
Amplicon235210
29368
225
225
SNP
C
b73
T
mo17


164
Amplicon235210
29369
275
275
SNP
C
mo17
T
b73


164
Amplicon235210
29370
283
283
SNP
A
b73
C
mo17


164
Amplicon235210
29371
392
392
SNP
G
mo17
T
b73


164
Amplicon235210
29372
406
406
SNP
A
mo17
G
b73


164
Amplicon235210
29373
418
418
SNP
A
b73
G
mo17


164
Amplicon235210
29374
422
422
IND
*
mo17
A
b73


164
Amplicon235210
29375
433
433
SNP
A
b73
G
mo17


164
Amplicon235210
29376
434
434
SNP
A
b73
G
mo17


164
Amplicon235210
29377
441
441
IND
*
mo17
A
b73


165
Amplicon235220
29390
302
302
SNP
C
b73
T
mo17


166
Amplicon235234
29435
555
555
SNP
A
b73
G
mo17


167
Amplicon235395
29745
1024
1024
SNP
C
b73
G
mo17


167
Amplicon235395
29746
1061
1061
SNP
C
mo17
T
b73


167
Amplicon235395
29747
1143
1143
SNP
A
mo17
G
b73


167
Amplicon235395
29748
1166
1166
IND
*
mo17
A
b73


168
Amplicon235410
29779
83
83
SNP
A
b73
T
mo17


168
Amplicon235410
29780
140
140
SNP
C
mo17
T
b73


168
Amplicon235410
29781
211
213
IND
***
mo17
GCT
b73


168
Amplicon235410
29782
313
313
SNP
A
b73
G
mo17


168
Amplicon235410
29783
315
315
SNP
C
mo17
T
b73


169
Amplicon235434
29819
64
64
SNP
C
b73
T
mo17


169
Amplicon235434
29820
108
108
SNP
A
b73
G
mo17


169
Amplicon235434
29821
120
120
SNP
A
mo17
G
b73


169
Amplicon235434
29822
121
121
SNP
A
mo17
T
b73


169
Amplicon235434
29823
180
180
SNP
C
mo17
T
b73


169
Amplicon235434
29824
186
186
SNP
A
mo17
G
b73


169
Amplicon235434
29825
202
202
SNP
A
b73
C
mo17


169
Amplicon235434
29826
210
210
SNP
A
mo17
G
b73


169
Amplicon235434
29827
215
215
SNP
C
b73
T
mo17


170
Amplicon235438
29829
161
161
SNP
A
mo17
G
b73


171
Amplicon235446
29832
136
136
SNP
C
mo17
T
b73


171
Amplicon235446
29833
247
247
SNP
C
b73
G
mo17


171
Amplicon235446
29834
255
255
SNP
A
b73
G
mo17


171
Amplicon235446
29835
303
303
SNP
A
mo17
T
b73


171
Amplicon235446
29836
304
304
SNP
C
b73
G
mo17


171
Amplicon235446
29837
435
435
SNP
A
mo17
T
b73


171
Amplicon235446
29838
501
511
IND
***********
mo17
GTATATATATT
b73


172
Amplicon235447
29839
101
101
SNP
C
mo17
G
b73


172
Amplicon235447
29840
106
106
SNP
C
b73
T
mo17


172
Amplicon235447
29841
107
107
SNP
A
mo17
T
b73


172
Amplicon235447
29842
260
260
SNP
A
b73
G
mo17


172
Amplicon235447
29843
285
285
SNP
A
b73
G
mo17


172
Amplicon235447
29844
286
286
IND
*
mo17
A
b73


172
Amplicon235447
29845
313
313
SNP
G
b73
T
mo17


172
Amplicon235447
29846
321
321
SNP
C
b73
T
mo17


172
Amplicon235447
29847
329
329
SNP
C
mo17
T
b73


172
Amplicon235447
29848
441
441
SNP
A
b73
T
mo17


172
Amplicon235447
29849
488
488
SNP
C
mo17
T
b73


173
Amplicon235455
29867
80
83
IND
****
mo17
TGAG
b73


173
Amplicon235455
29868
194
195
IND
**
mo17
AA
b73


173
Amplicon235455
29869
362
362
SNP
A
b73
G
mo17


173
Amplicon235455
29870
364
364
SNP
C
mo17
G
b73


173
Amplicon235455
29871
374
374
SNP
A
mo17
C
b73


174
Amplicon235516
30000
152
152
SNP
A
b73
C
mo17


175
Amplicon235541
30025
170
170
SNP
C
b73
T
mo17


175
Amplicon235541
30026
196
196
SNP
C
b73
T
mo17


175
Amplicon235541
30027
265
265
SNP
C
b73
T
mo17


175
Amplicon235541
30028
283
283
SNP
C
mo17
G
b73


175
Amplicon235541
30029
316
316
SNP
C
mo17
T
b73


176
Amplicon235586
30174
674
674
SNP
C
mo17
T
b73


176
Amplicon235586
30175
699
699
IND
*
b73
C
mo17


176
Amplicon235586
30176
733
733
SNP
A
mo17
T
b73


177
Amplicon235641
30270
1134
1134
SNP
C
mo17
T
b73


178
Amplicon235855
30673
56
56
SNP
C
mo17
G
b73


178
Amplicon235855
30674
136
136
SNP
C
mo17
T
b73


178
Amplicon235855
30675
182
182
SNP
A
b73
G
mo17


178
Amplicon235855
30676
188
188
SNP
C
mo17
T
b73


178
Amplicon235855
30677
194
194
SNP
C
b73
T
mo17


178
Amplicon235855
30678
236
236
SNP
A
b73
C
mo17


178
Amplicon235855
30679
305
305
SNP
C
b73
T
mo17


179
Amplicon235879
30745
1028
1028
SNP
C
mo17
T
b73


180
Amplicon235892
30771
165
165
SNP
C
mo17
T
b73


181
Amplicon235914
30835
131
131
SNP
A
mo17
G
b73


181
Amplicon235914
30836
168
168
SNP
G
b73
T
mo17


181
Amplicon235914
30837
201
201
SNP
A
mo17
G
b73


181
Amplicon235914
30838
213
213
SNP
C
b73
T
mo17


181
Amplicon235914
30839
269
269
SNP
A
mo17
G
b73


181
Amplicon235914
30840
412
412
SNP
A
b73
G
mo17


182
Amplicon235959
30871
294
294
SNP
A
b73
G
mo17


182
Amplicon235959
30872
811
811
SNP
C
b73
T
mo17


183
Amplicon236049
31050
33
33
SNP
A
b73
C
mo17


183
Amplicon236049
31051
35
35
SNP
A
b73
C
mo17


183
Amplicon236049
31052
37
37
SNP
A
b73
G
mo17


183
Amplicon236049
31053
46
46
SNP
A
mo17
T
b73


183
Amplicon236049
31054
47
47
SNP
A
mo17
G
b73


183
Amplicon236049
31055
48
48
SNP
C
b73
G
mo17


183
Amplicon236049
31056
51
51
SNP
A
b73
T
mo17


183
Amplicon236049
31057
53
53
SNP
C
b73
T
mo17


183
Amplicon236049
31058
54
54
SNP
A
b73
C
mo17


183
Amplicon236049
31059
55
55
SNP
A
b73
C
mo17


183
Amplicon236049
31060
56
56
SNP
G
b73
T
mo17


183
Amplicon236049
31061
58
58
SNP
C
b73
G
mo17


183
Amplicon236049
31062
62
62
SNP
C
b73
T
mo17


183
Amplicon236049
31063
64
65
IND
**
mo17
TC
b73


183
Amplicon236049
31064
125
125
SNP
A
b73
C
mo17


183
Amplicon236049
31065
179
179
SNP
C
mo17
G
b73


183
Amplicon236049
31066
539
539
SNP
G
mo17
T
b73


184
Amplicon236057
31084
52
52
SNP
C
b73
G
mo17


184
Amplicon236057
31085
297
297
SNP
A
b73
G
mo17


185
Amplicon236120
31233
1066
1066
SNP
A
mo17
C
b73


186
Amplicon236181
31370
432
432
SNP
G
mo17
T
b73


187
Amplicon236216
31473
760
760
SNP
A
b73
G
mo17


187
Amplicon236216
31474
843
843
SNP
A
mo17
T
b73


187
Amplicon236216
31475
938
938
SNP
C
mo17
G
b73


187
Amplicon236216
31476
1016
1016
IND
*
mo17
T
b73


187
Amplicon236216
31477
1058
1058
SNP
A
mo17
G
b73


188
Amplicon236259
31546
306
306
SNP
A
mo17
C
b73


188
Amplicon236259
31547
473
473
SNP
C
mo17
T
b73


188
Amplicon236259
31548
482
482
SNP
A
b73
G
mo17


189
Amplicon236326
31684
259
259
SNP
A
b73
T
mo17


190
Amplicon236386
31790
510
510
SNP
A
mo17
T
b73


191
Amplicon236471
31993
365
365
SNP
A
mo17
G
b73


191
Amplicon236471
31994
367
367
IND
*
mo17
C
b73


192
Amplicon236482
32014
68
68
SNP
A
mo17
G
b73


192
Amplicon236482
32015
93
93
SNP
C
b73
G
mo17


192
Amplicon236482
32016
196
196
SNP
A
b73
G
mo17


192
Amplicon236482
32017
674
674
SNP
G
b73
T
mo17


193
Amplicon236492
32028
81
81
SNP
C
mo17
T
b73


193
Amplicon236492
32029
102
102
SNP
C
mo17
G
b73


193
Amplicon236492
32030
162
164
IND
***
b73
TGG
mo17


193
Amplicon236492
32031
192
197
IND
******
b73
TGCCGG
mo17


193
Amplicon236492
32032
310
310
SNP
A
mo17
C
b73


193
Amplicon236492
32033
435
435
SNP
C
b73
T
mo17


194
Amplicon236494
32034
497
497
SNP
A
mo17
G
b73


195
Amplicon236499
32049
182
182
SNP
C
b73
T
mo17


195
Amplicon236499
32050
401
401
SNP
C
mo17
T
b73


195
Amplicon236499
32051
402
402
SNP
A
mo17
G
b73


196
Amplicon236541
32137
257
257
IND
*
b73
A
mo17


196
Amplicon236541
32138
419
429
IND
***********
mo17
CCGATCCATCT
b73


197
Amplicon236590
32244
26
26
SNP
C
b73
T
mo17


197
Amplicon236590
32245
81
81
SNP
A
b73
G
mo17


197
Amplicon236590
32246
91
97
IND
*******
mo17
AGTGCTG
b73


197
Amplicon236590
32247
161
161
SNP
C
b73
T
mo17


197
Amplicon236590
32248
274
274
SNP
C
b73
T
mo17


198
Amplicon236597
32252
36
36
SNP
C
b73
G
mo17


198
Amplicon236597
32253
43
43
SNP
A
b73
C
mo17


198
Amplicon236597
32254
117
117
SNP
C
mo17
T
b73


198
Amplicon236597
32255
288
288
SNP
C
mo17
G
b73


198
Amplicon236597
32256
318
318
SNP
A
mo17
G
b73


198
Amplicon236597
32257
345
345
SNP
A
mo17
G
b73


198
Amplicon236597
32258
351
351
SNP
G
b73
T
mo17


198
Amplicon236597
32259
364
364
SNP
C
mo17
T
b73


198
Amplicon236597
32260
407
407
SNP
C
b73
G
mo17


198
Amplicon236597
32261
450
450
SNP
C
mo17
T
b73


198
Amplicon236597
32262
458
458
SNP
A
mo17
G
b73


198
Amplicon236597
32263
582
582
SNP
A
b73
G
mo17


198
Amplicon236597
32264
589
589
SNP
A
b73
G
mo17


198
Amplicon236597
32265
604
604
SNP
C
mo17
T
b73


198
Amplicon236597
32266
848
848
SNP
A
b73
G
mo17


198
Amplicon236597
32267
865
865
SNP
C
b73
T
mo17


199
Amplicon236600
32272
260
260
SNP
C
mo17
T
b73


200
Amplicon236692
32428
101
101
SNP
C
mo17
T
b73


200
Amplicon236692
32429
112
112
SNP
A
mo17
G
b73


200
Amplicon236692
32430
202
202
SNP
A
mo17
G
b73


200
Amplicon236692
32431
250
250
SNP
C
b73
T
mo17


201
Amplicon276424
33246
113
113
SNP
G
mo17
T
b73


201
Amplicon276424
33247
120
120
SNP
A
mo17
G
b73


201
Amplicon276424
33248
140
140
SNP
A
b73
G
mo17


201
Amplicon276424
33249
190
190
SNP
C
mo17
T
b73


201
Amplicon276424
33250
231
231
IND
*
b73
A
mo17


201
Amplicon276424
33251
231
232
IND
**
b73
AT
mo17


201
Amplicon276424
33252
243
243
SNP
G
b73
T
mo17


201
Amplicon276424
33253
296
296
SNP
G
b73
T
mo17


201
Amplicon276424
33254
340
340
IND
*
b73
G
mo17


201
Amplicon276424
33255
341
342
IND
**
b73
CC
mo17


202
Amplicon276456
33315
93
93
SNP
A
b73
G
mo17


202
Amplicon276456
33316
111
111
SNP
C
b73
T
mo17


202
Amplicon276456
33317
118
118
SNP
A
b73
T
mo17


202
Amplicon276456
33318
151
153
IND
***
mo17
TGT
b73


202
Amplicon276456
33319
154
157
IND
****
mo17
TCTG
b73


202
Amplicon276456
33320
208
208
SNP
C
b73
G
mo17


202
Amplicon276456
33321
241
241
SNP
A
mo17
G
b73


202
Amplicon276456
33322
396
396
SNP
C
b73
T
mo17


203
Amplicon276493
33371
163
163
IND
*
mo17
G
b73


203
Amplicon276493
33372
238
238
SNP
A
b73
C
mo17


204
Amplicon276497
33373
95
95
SNP
C
mo17
T
b73


204
Amplicon276497
33374
127
127
SNP
C
mo17
T
b73


204
Amplicon276497
33375
130
130
SNP
C
mo17
T
b73


204
Amplicon276497
33376
362
362
SNP
C
b73
G
mo17


204
Amplicon276497
33377
370
370
SNP
G
b73
T
mo17


205
Amplicon276744
33764
728
728
SNP
A
mo17
G
b73


205
Amplicon276744
33765
835
835
SNP
C
b73
T
mo17


205
Amplicon276744
33766
874
874
SNP
A
b73
T
mo17


205
Amplicon276744
33767
919
919
SNP
A
mo17
T
b73


205
Amplicon276744
33768
1014
1014
SNP
A
b73
T
mo17


205
Amplicon276744
33769
1078
1081
IND
****
mo17
TAGC
b73


206
Amplicon276782
33816
252
252
SNP
C
mo17
T
b73


206
Amplicon276782
33819
319
319
SNP
A
mo17
G
b73


207
Amplicon277037
34205
117
117
SNP
C
mo17
T
b73


207
Amplicon277037
34206
248
248
SNP
A
b73
T
mo17


208
Amplicon277306
34556
519
519
SNP
A
b73
G
mo17


208
Amplicon277306
34557
558
558
SNP
A
mo17
G
b73


208
Amplicon277306
34558
576
576
SNP
A
b73
G
mo17


208
Amplicon277306
34559
625
625
SNP
C
mo17
T
b73


208
Amplicon277306
34560
681
681
SNP
A
b73
G
mo17


208
Amplicon277306
34561
770
770
SNP
C
mo17
T
b73


208
Amplicon277306
34562
771
771
IND
*
b73
A
mo17


208
Amplicon277306
34563
788
788
SNP
C
b73
T
mo17


208
Amplicon277306
34564
852
852
SNP
A
b73
G
mo17


209
Amplicon277511
34895
47
47
SNP
C
b73
G
mo17


209
Amplicon277511
34896
48
48
SNP
C
b73
T
mo17


209
Amplicon277511
34897
52
52
IND
*
b73
C
mo17


209
Amplicon277511
34898
52
53
IND
**
b73
C*
mo17


209
Amplicon277511
34899
75
75
SNP
C
b73
T
mo17


209
Amplicon277511
34900
307
307
SNP
A
b73
C
mo17


209
Amplicon277511
34901
344
344
SNP
A
mo17
G
b73


209
Amplicon277511
34902
347
347
SNP
C
b73
T
mo17


209
Amplicon277511
34903
408
408
SNP
C
mo17
T
b73


210
Amplicon277716
35173
659
659
IND
*
mo17
G
b73


210
Amplicon277716
35174
767
767
SNP
C
b73
T
mo17


210
Amplicon277716
35175
770
770
SNP
A
b73
G
mo17


211
Amplicon277824
35294
186
186
SNP
A
mo17
G
b73


212
Amplicon277832
35295
102
102
SNP
C
b73
T
mo17


212
Amplicon277832
35296
193
193
SNP
A
mo17
C
b73


212
Amplicon277832
35297
206
206
SNP
A
b73
G
mo17


212
Amplicon277832
35298
218
218
IND
*
b73
C
mo17


212
Amplicon277832
35299
220
222
IND
***
b73
GCT
mo17


212
Amplicon277832
35300
265
265
SNP
C
mo17
T
b73


212
Amplicon277832
35301
277
279
IND
***
mo17
ATG
b73


212
Amplicon277832
35302
449
449
SNP
C
b73
T
mo17


213
Amplicon277876
35338
104
104
SNP
C
mo17
G
b73


213
Amplicon277876
35339
329
333
IND
*****
b73
CAAAG
mo17


213
Amplicon277876
35340
367
367
SNP
A
b73
G
mo17


214
Amplicon277914
35377
66
66
SNP
C
b73
G
mo17


215
Amplicon277962
35407
31
31
SNP
A
mo17
G
b73


215
Amplicon277962
35408
220
220
SNP
A
mo17
C
b73


215
Amplicon277962
35409
292
292
SNP
A
b73
C
mo17


215
Amplicon277962
35410
339
339
SNP
A
mo17
G
b73


216
Amplicon277971
35413
48
48
SNP
A
b73
C
mo17


216
Amplicon277971
35414
58
58
SNP
A
mo17
G
b73


216
Amplicon277971
35415
160
161
IND
**
mo17
TA
b73


216
Amplicon277971
35416
163
163
SNP
G
b73
T
mo17


216
Amplicon277971
35417
175
175
SNP
A
b73
G
mo17


216
Amplicon277971
35418
254
254
IND
*
mo17
T
b73


217
Amplicon278078
35567
278
278
SNP
G
b73
T
mo17


217
Amplicon278078
35568
297
297
SNP
A
mo17
G
b73


217
Amplicon278078
35569
331
331
IND
*
b73
G
mo17


218
Amplicon278086
35574
384
384
SNP
C
mo17
T
b73


219
Amplicon278122
35631
115
115
SNP
C
mo17
T
b73


219
Amplicon278122
35632
267
267
SNP
A
b73
G
mo17


219
Amplicon278122
35633
318
318
SNP
A
b73
C
mo17


220
Amplicon310557
36022
77
77
SNP
C
b73
G
mo17


220
Amplicon310557
36023
80
80
SNP
G
b73
T
mo17


220
Amplicon310557
36024
269
269
SNP
A
mo17
G
b73


220
Amplicon310557
36025
283
283
SNP
G
mo17
T
b73


220
Amplicon310557
36026
353
353
IND
*
mo17
A
b73


220
Amplicon310557
36027
353
354
IND
**
mo17
AT
b73


221
Amplicon310599
36062
94
94
SNP
A
mo17
C
b73


221
Amplicon310599
36063
95
95
SNP
A
mo17
C
b73


221
Amplicon310599
36064
128
128
SNP
C
mo17
G
b73


221
Amplicon310599
36065
129
129
SNP
A
mo17
G
b73


221
Amplicon310599
36066
135
135
SNP
G
mo17
T
b73


221
Amplicon310599
36067
144
144
SNP
C
b73
T
mo17


221
Amplicon310599
36068
279
279
SNP
G
b73
T
mo17


221
Amplicon310599
36069
280
280
SNP
C
mo17
T
b73


221
Amplicon310599
36070
300
300
IND
*
b73
A
mo17


221
Amplicon310599
36071
324
324
SNP
A
b73
T
mo17


221
Amplicon310599
36072
367
367
SNP
A
mo17
G
b73


221
Amplicon310599
36073
403
403
SNP
A
mo17
G
b73


221
Amplicon310599
36074
422
422
SNP
C
mo17
T
b73


221
Amplicon310599
36075
426
426
SNP
C
mo17
T
b73


221
Amplicon310599
36076
465
465
SNP
C
mo17
T
b73


222
Amplicon310656
36195
119
119
SNP
A
mo17
C
b73


222
Amplicon310656
36196
122
122
SNP
C
b73
T
mo17


222
Amplicon310656
36197
134
134
SNP
A
b73
C
mo17


222
Amplicon310656
36198
146
146
SNP
A
mo17
C
b73


222
Amplicon310656
36199
182
182
SNP
A
mo17
C
b73


222
Amplicon310656
36200
213
218
IND
******
mo17
AGCGAC
b73


222
Amplicon310656
36201
239
239
SNP
A
b73
G
mo17


222
Amplicon310656
36202
317
317
SNP
C
b73
G
mo17


223
Amplicon310739
36286
335
336
IND
**
mo17
AT
b73


223
Amplicon310739
36287
435
436
IND
**
b73
CT
mo17


223
Amplicon310739
36288
455
455
SNP
A
b73
G
mo17


224
Amplicon310755
36323
240
240
SNP
G
b73
T
mo17


225
Amplicon310817
36448
201
201
SNP
C
mo17
T
b73


225
Amplicon310817
36449
252
254
IND
***
mo17
AGC
b73


226
Amplicon310854
36487
201
203
IND
***
b73
TGG
mo17


226
Amplicon310854
36488
227
228
IND
**
b73
AT
mo17


226
Amplicon310854
36489
235
235
IND
*
mo17
T
b73


226
Amplicon310854
36490
243
243
SNP
G
b73
T
mo17


226
Amplicon310854
36491
272
274
IND
***
b73
TAG
mo17


226
Amplicon310854
36492
272
275
IND
****
b73
TAGC
mo17


226
Amplicon310854
36493
315
316
IND
**
mo17
GA
b73


226
Amplicon310854
36494
319
319
SNP
C
b73
T
mo17


227
Amplicon311004
36636
637
637
SNP
A
mo17
G
b73


227
Amplicon311004
36637
698
698
SNP
C
mo17
T
b73


227
Amplicon311004
36638
957
957
SNP
G
mo17
T
b73


227
Amplicon311004
36639
958
958
SNP
G
mo17
T
b73


227
Amplicon311004
36640
968
968
SNP
A
b73
G
mo17


227
Amplicon311004
36641
1085
1085
SNP
A
mo17
C
b73


227
Amplicon311004
36642
1086
1086
SNP
A
mo17
G
b73


227
Amplicon311004
36643
1127
1127
SNP
A
b73
G
mo17


228
Amplicon311038
36685
202
202
SNP
A
mo17
G
b73


228
Amplicon311038
36686
444
444
SNP
A
mo17
T
b73


228
Amplicon311038
36687
515
515
SNP
C
b73
T
mo17


229
Amplicon311045
36694
179
179
SNP
C
mo17
T
b73


229
Amplicon311045
36695
242
242
SNP
C
b73
G
mo17


230
Amplicon311184
36862
296
296
SNP
A
b73
T
mo17


230
Amplicon311184
36863
326
326
SNP
C
b73
T
mo17


230
Amplicon311184
36864
374
374
SNP
C
b73
T
mo17


230
Amplicon311184
36865
507
507
SNP
A
mo17
C
b73


230
Amplicon311184
36866
547
547
SNP
C
b73
T
mo17


231
Amplicon311375
37066
105
105
SNP
C
b73
T
mo17


231
Amplicon311375
37067
127
127
SNP
A
b73
G
mo17


231
Amplicon311375
37068
163
171
IND
*********
mo17
GATACGGCG
b73


232
Amplicon311650
37492
125
125
IND
*
mo17
G
b73


232
Amplicon311650
37493
130
130
SNP
A
mo17
G
b73


232
Amplicon311650
37494
204
204
SNP
A
mo17
G
b73


232
Amplicon311650
37495
211
211
SNP
A
b73
G
mo17


232
Amplicon311650
37496
212
212
SNP
A
b73
G
mo17


232
Amplicon311650
37497
221
221
IND
*
mo17
G
b73


232
Amplicon311650
37498
236
236
SNP
G
mo17
T
b73


232
Amplicon311650
37499
239
242
IND
****
mo17
CTTG
b73


232
Amplicon311650
37500
359
362
IND
****
mo17
TGCC
b73


232
Amplicon311650
37501
410
410
SNP
A
mo17
C
b73


232
Amplicon311650
37502
411
411
SNP
A
b73
C
mo17


232
Amplicon311650
37503
426
426
SNP
G
mo17
T
b73


232
Amplicon311650
37504
465
471
IND
*******
b73
AGTGCTG
mo17


232
Amplicon311650
37505
535
536
IND
**
mo17
CT
b73


232
Amplicon311650
37506
541
541
SNP
C
b73
T
mo17


232
Amplicon311650
37507
610
610
IND
*
mo17
C
b73


233
Amplicon311695
37555
394
394
SNP
C
b73
T
mo17


233
Amplicon311695
37556
505
505
SNP
A
mo17
G
b73


234
Amplicon311738
37631
271
271
SNP
C
mo17
G
b73


234
Amplicon311738
37632
333
340
IND
********
mo17
CGTTCTAA
b73


234
Amplicon311738
37633
389
397
IND
*********
b73
CGTTGGGGG
mo17


234
Amplicon311738
37634
542
542
SNP
G
mo17
T
b73


235
Amplicon346446
37684
397
397
SNP
C
b73
T
mo17


235
Amplicon346446
37685
407
407
SNP
A
mo17
C
b73


235
Amplicon346446
37686
417
417
SNP
A
mo17
T
b73


235
Amplicon346446
37687
483
483
IND
*
mo17
G
b73


235
Amplicon346446
37688
562
562
SNP
C
b73
T
mo17


235
Amplicon346446
37689
685
691
IND
*******
mo17
ATTATAA
b73


236
Amplicon346472
37715
392
392
SNP
A
b73
G
mo17


236
Amplicon346472
37716
512
512
SNP
C
b73
T
mo17


236
Amplicon346472
37717
522
522
IND
*
mo17
A
b73


236
Amplicon346472
37718
563
563
SNP
G
mo17
T
b73


236
Amplicon346472
37719
573
576
IND
****
b73
ACGA
mo17


237
Amplicon346640
37946
41
41
SNP
A
mo17
G
b73


237
Amplicon346640
37947
86
86
SNP
A
b73
G
mo17


237
Amplicon346640
37948
134
134
SNP
G
b73
T
mo17


237
Amplicon346640
37949
191
191
SNP
A
mo17
G
b73


237
Amplicon346640
37950
194
194
SNP
C
b73
T
mo17


237
Amplicon346640
37951
197
197
SNP
C
b73
T
mo17


237
Amplicon346640
37952
199
199
SNP
A
mo17
G
b73


237
Amplicon346640
37953
229
232
IND
****
mo17
TATA
b73


237
Amplicon346640
37954
284
284
IND
*
b73
C
mo17


237
Amplicon346640
37955
286
286
IND
*
b73
A
mo17


237
Amplicon346640
37956
288
288
SNP
A
b73
C
mo17


237
Amplicon346640
37957
343
343
SNP
A
mo17
G
b73


237
Amplicon346640
37958
368
368
IND
*
mo17
C
b73


237
Amplicon346640
37959
392
392
SNP
G
mo17
T
b73


237
Amplicon346640
37960
393
394
IND
**
mo17
TT
b73


237
Amplicon346640
37961
397
397
SNP
A
b73
G
mo17


237
Amplicon346640
37962
410
410
SNP
A
mo17
C
b73


237
Amplicon346640
37963
411
411
SNP
A
mo17
G
b73


237
Amplicon346640
37964
450
450
SNP
G
mo17
T
b73


237
Amplicon346640
37965
514
514
SNP
A
mo17
G
b73


237
Amplicon346640
37966
552
552
SNP
C
b73
G
mo17


237
Amplicon346640
37967
773
773
SNP
A
mo17
C
b73


238
Amplicon346980
38478
262
262
SNP
C
mo17
T
b73


238
Amplicon346980
38479
400
400
SNP
A
b73
C
mo17


238
Amplicon346980
38480
549
554
IND
******
b73
TCGCTC
mo17


239
Amplicon347076
38604
172
172
SNP
A
mo17
G
b73


239
Amplicon347076
38605
207
207
SNP
C
mo17
T
b73


239
Amplicon347076
38606
224
224
SNP
A
mo17
G
b73


239
Amplicon347076
38607
252
253
IND
**
b73
GG
mo17


239
Amplicon347076
38608
380
380
SNP
A
mo17
G
b73


239
Amplicon347076
38609
387
387
SNP
A
b73
G
mo17


239
Amplicon347076
38610
432
432
SNP
G
b73
T
mo17


239
Amplicon347076
38611
505
505
SNP
A
mo17
G
b73


239
Amplicon347076
38612
506
506
SNP
A
mo17
G
b73


239
Amplicon347076
38613
515
515
IND
*
b73
T
mo17


239
Amplicon347076
38614
521
521
IND
*
b73
T
mo17


239
Amplicon347076
38615
587
592
IND
******
b73
CCCCCA
mo17


239
Amplicon347076
38616
599
599
SNP
A
mo17
G
b73


239
Amplicon347076
38617
611
611
SNP
A
b73
G
mo17


239
Amplicon347076
38618
614
614
SNP
G
b73
T
mo17


240
Amplicon347095
38652
23
23
IND
*
b73
A
mo17


240
Amplicon347095
38653
200
200
SNP
A
mo17
T
b73


240
Amplicon347095
38654
414
414
SNP
A
mo17
G
b73


240
Amplicon347095
38655
599
599
SNP
C
mo17
T
b73


240
Amplicon347095
38656
682
682
SNP
A
b73
T
mo17


241
Amplicon347114
38700
228
228
SNP
C
b73
T
mo17


241
Amplicon347114
38701
303
308
IND
******
b73
AATCAT
mo17


242
Amplicon347251
38848
39
39
SNP
G
b73
T
mo17


242
Amplicon347251
38849
83
83
SNP
A
mo17
T
b73


242
Amplicon347251
38850
262
262
SNP
G
b73
T
mo17


242
Amplicon347251
38851
263
264
IND
**
b73
TT
mo17


242
Amplicon347251
38852
550
550
SNP
C
b73
G
mo17


243
Amplicon347285
38909
41
41
SNP
A
b73
T
mo17


243
Amplicon347285
38910
93
96
IND
****
mo17
TGCA
b73


243
Amplicon347285
38911
99
99
SNP
A
b73
G
mo17


243
Amplicon347285
38912
100
100
SNP
C
b73
T
mo17


243
Amplicon347285
38913
105
105
SNP
A
mo17
C
b73


243
Amplicon347285
38914
128
131
IND
****
mo17
ATTA
b73


243
Amplicon347285
38915
148
148
SNP
A
mo17
G
b73


243
Amplicon347285
38916
152
152
SNP
A
mo17
C
b73


243
Amplicon347285
38917
158
158
SNP
C
b73
T
mo17


243
Amplicon347285
38918
175
175
SNP
A
mo17
G
b73


243
Amplicon347285
38919
180
180
IND
*
mo17
G
b73


243
Amplicon347285
38920
280
280
SNP
C
b73
T
mo17


243
Amplicon347285
38921
375
375
SNP
C
b73
G
mo17


243
Amplicon347285
38922
511
511
SNP
G
b73
T
mo17


243
Amplicon347285
38923
517
517
SNP
C
mo17
T
b73


244
Amplicon347403
39063
80
80
SNP
A
mo17
G
b73


244
Amplicon347403
39064
316
316
SNP
C
mo17
T
b73


245
Amplicon347507
39266
89
93
IND
*****
b73
CTCCC
mo17


245
Amplicon347507
39267
118
118
SNP
A
mo17
G
b73


245
Amplicon347507
39268
126
126
SNP
C
mo17
T
b73


245
Amplicon347507
39269
165
165
SNP
C
mo17
T
b73


245
Amplicon347507
39270
174
174
SNP
C
b73
G
mo17


245
Amplicon347507
39271
251
251
SNP
G
b73
T
mo17


245
Amplicon347507
39272
306
319
IND
**************
mo17
ACCTGACCCGCGGC
b73


245
Amplicon347507
39273
306
320
IND
***************
mo17
ACCTGACCCGCGGCG
b73


245
Amplicon347507
39274
361
361
SNP
C
mo17
G
b73


245
Amplicon347507
39275
396
396
SNP
C
b73
T
mo17


245
Amplicon347507
39276
505
505
SNP
C
b73
G
mo17


245
Amplicon347507
39277
507
507
SNP
C
b73
G
mo17


245
Amplicon347507
39278
508
508
SNP
A
b73
C
mo17


245
Amplicon347507
39279
509
509
SNP
A
b73
G
mo17


245
Amplicon347507
39280
510
510
SNP
C
mo17
G
b73


245
Amplicon347507
39281
511
511
SNP
A
mo17
C
b73


245
Amplicon347507
39282
512
512
SNP
G
mo17
T
b73


245
Amplicon347507
39283
516
516
SNP
A
mo17
T
b73


246
Amplicon347517
39286
405
405
SNP
C
b73
T
mo17


246
Amplicon347517
39287
424
424
SNP
A
mo17
G
b73


246
Amplicon347517
39288
436
436
SNP
A
mo17
G
b73


246
Amplicon347517
39289
500
500
SNP
C
b73
T
mo17


247
Amplicon347532
39348
485
485
SNP
A
b73
T
mo17


247
Amplicon347532
39349
488
488
SNP
A
b73
G
mo17


247
Amplicon347532
39350
594
594
SNP
C
mo17
T
b73


247
Amplicon347532
39351
680
680
SNP
A
mo17
G
b73


247
Amplicon347532
39352
700
700
IND
*
b73
T
mo17


247
Amplicon347532
39353
714
714
SNP
G
mo17
T
b73


247
Amplicon347532
39354
729
729
SNP
C
b73
G
mo17


248
Amplicon347593
39502
51
51
SNP
A
b73
G
mo17


249
Amplicon347598
39507
137
137
SNP
C
mo17
T
b73


249
Amplicon347598
39508
433
434
IND
**
mo17
CC
b73


249
Amplicon347598
39509
477
479
IND
***
b73
GCT
mo17


249
Amplicon347598
39510
500
508
IND
*********
b73
ATGGCAGGC
mo17


249
Amplicon347598
39511
559
559
SNP
C
mo17
G
b73


250
Amplicon347833
39773
47
47
SNP
C
b73
T
mo17


250
Amplicon347833
39774
91
91
IND
*
mo17
G
b73


250
Amplicon347833
39775
117
117
SNP
A
b73
T
mo17


250
Amplicon347833
39776
153
153
SNP
A
b73
C
mo17


250
Amplicon347833
39777
164
164
SNP
A
b73
G
mo17


250
Amplicon347833
39778
545
546
IND
**
b73
TT
mo17


250
Amplicon347833
39779
568
568
SNP
C
mo17
T
b73


250
Amplicon347833
39780
579
579
SNP
G
mo17
T
b73


250
Amplicon347833
39781
580
580
SNP
A
b73
C
mo17


251
Amplicon347845
39782
322
322
SNP
C
mo17
G
b73


251
Amplicon347845
39783
359
359
SNP
C
mo17
T
b73


251
Amplicon347845
39784
385
385
SNP
C
mo17
T
b73


251
Amplicon347845
39785
515
515
SNP
G
b73
T
mo17


251
Amplicon347845
39786
569
569
SNP
A
mo17
C
b73


251
Amplicon347845
39787
719
719
IND
*
mo17
G
b73


251
Amplicon347845
39788
724
724
IND
*
mo17
T
b73


252
Amplicon347958
39886
118
118
IND
*
b73
G
mo17


252
Amplicon347958
39887
220
220
SNP
A
b73
C
mo17


252
Amplicon347958
39888
275
275
SNP
C
mo17
G
b73


252
Amplicon347958
39889
290
290
SNP
G
b73
T
mo17


252
Amplicon347958
39890
308
308
SNP
C
mo17
T
b73


252
Amplicon347958
39891
335
335
SNP
C
b73
G
mo17


252
Amplicon347958
39892
379
379
IND
*
b73
C
mo17


252
Amplicon347958
39893
402
402
SNP
A
mo17
G
b73


252
Amplicon347958
39894
435
435
SNP
A
mo17
C
b73


252
Amplicon347958
39895
483
483
SNP
G
mo17
T
b73


252
Amplicon347958
39896
651
651
SNP
A
mo17
C
b73


253
Amplicon348012
39978
226
226
SNP
C
b73
T
mo17


254
Amplicon389945
40124
212
212
SNP
A
b73
G
mo17


255
Amplicon390056
40189
324
324
SNP
C
mo17
T
b73


256
Amplicon390137
40320
319
319
SNP
C
b73
T
mo17


257
Amplicon390154
40337
106
106
SNP
C
mo17
G
b73


257
Amplicon390154
40338
457
457
SNP
G
mo17
T
b73


258
Amplicon390253
40431
243
243
SNP
C
b73
T
mo17


258
Amplicon390253
40432
245
245
SNP
C
b73
T
mo17


258
Amplicon390253
40433
246
246
IND
*
b73
T
mo17


259
Amplicon390282
40451
23
24
IND
**
mo17
CA
b73


259
Amplicon390282
40452
28
28
SNP
C
mo17
G
b73


259
Amplicon390282
40453
146
149
IND
****
b73
CTAG
mo17


259
Amplicon390282
40454
201
204
IND
****
b73
TATA
mo17


259
Amplicon390282
40455
222
222
SNP
A
b73
G
mo17


259
Amplicon390282
40456
242
242
SNP
A
mo17
T
b73


259
Amplicon390282
40457
266
266
SNP
C
mo17
T
b73


259
Amplicon390282
40458
270
270
SNP
C
b73
T
mo17


259
Amplicon390282
40459
296
296
SNP
A
mo17
C
b73


259
Amplicon390282
40460
309
311
IND
***
mo17
TTT
b73


259
Amplicon390282
40461
365
365
SNP
A
mo17
T
b73


259
Amplicon390282
40462
395
395
SNP
A
mo17
G
b73


259
Amplicon390282
40463
399
399
IND
*
b73
A
mo17


259
Amplicon390282
40464
399
400
IND
**
b73
AT
mo17


260
Amplicon390291
40473
281
281
SNP
A
mo17
C
b73


260
Amplicon390291
40474
397
397
IND
*
mo17
C
b73


260
Amplicon390291
40475
407
407
SNP
C
mo17
T
b73


260
Amplicon390291
40476
408
408
SNP
A
b73
C
mo17


260
Amplicon390291
40477
409
409
IND
*
mo17
T
b73


260
Amplicon390291
40478
417
418
IND
**
mo17
CA
b73


261
Amplicon390465
40655
58
58
SNP
A
b73
G
mo17


262
Amplicon390649
40931
355
357
IND
***
b73
CTT
mo17


263
Amplicon390853
41280
224
224
SNP
C
b73
T
mo17


263
Amplicon390853
41281
283
283
SNP
C
mo17
G
b73


264
Amplicon391267
41850
54
54
SNP
C
b73
T
mo17


264
Amplicon391267
41851
111
111
SNP
C
b73
G
mo17


264
Amplicon391267
41852
119
119
SNP
A
mo17
T
b73


265
Amplicon391526
42161
133
133
SNP
G
mo17
T
b73


265
Amplicon391526
42162
193
193
SNP
A
b73
G
mo17


265
Amplicon391526
42163
253
253
SNP
A
mo17
G
b73


265
Amplicon391526
42164
319
319
SNP
A
b73
G
mo17


265
Amplicon391526
42165
349
349
SNP
C
mo17
T
b73


265
Amplicon391526
42166
373
373
SNP
A
mo17
G
b73


266
Amplicon391532
42173
90
91
IND
**
mo17
TA
b73


266
Amplicon391532
42174
122
122
IND
*
b73
G
mo17


266
Amplicon391532
42175
128
129
IND
**
b73
AA
mo17


266
Amplicon391532
42176
237
238
IND
**
mo17
GC
b73


266
Amplicon391532
42177
278
286
IND
*********
mo17
GCCTTGTTG
b73


266
Amplicon391532
42178
317
318
IND
**
b73
CT
mo17


266
Amplicon391532
42179
320
323
IND
****
b73
CGAC
mo17


266
Amplicon391532
42180
334
334
SNP
C
mo17
G
b73


266
Amplicon391532
42181
345
347
IND
***
b73
CTA
mo17


266
Amplicon391532
42182
360
360
SNP
A
mo17
G
b73


266
Amplicon391532
42183
371
371
IND
*
b73
G
mo17


266
Amplicon391532
42184
375
375
IND
*
b73
A
mo17


266
Amplicon391532
42185
383
383
SNP
G
b73
T
mo17


266
Amplicon391532
42186
400
400
SNP
A
mo17
T
b73


266
Amplicon391532
42187
405
405
IND
*
b73
A
mo17


266
Amplicon391532
42188
414
416
IND
***
b73
ATA
mo17


266
Amplicon391532
42189
433
433
SNP
A
b73
T
mo17


266
Amplicon391532
42190
443
446
IND
****
b73
TGTA
mo17


266
Amplicon391532
42191
448
448
IND
*
b73
C
mo17


267
Amplicon437734
42930
136
136
SNP
A
mo17
C
b73


267
Amplicon437734
42931
195
195
SNP
C
b73
T
mo17


267
Amplicon437734
42932
297
297
SNP
A
b73
G
mo17


267
Amplicon437734
42933
338
338
SNP
A
b73
G
mo17


267
Amplicon437734
42934
421
421
SNP
A
b73
G
mo17


267
Amplicon437734
42935
427
427
SNP
C
b73
T
mo17


268
Amplicon437882
43119
80
80
SNP
C
b73
G
mo17


268
Amplicon437882
43120
141
141
SNP
C
b73
T
mo17


268
Amplicon437882
43121
310
310
SNP
A
b73
G
mo17


268
Amplicon437882
43122
354
354
SNP
C
b73
G
mo17


268
Amplicon437882
43123
410
410
SNP
A
mo17
G
b73


269
Amplicon437962
43220
44
44
SNP
G
mo17
T
b73


269
Amplicon437962
43221
73
76
IND
****
mo17
CTAG
b73


269
Amplicon437962
43222
114
114
SNP
C
mo17
T
b73


269
Amplicon437962
43223
119
119
IND
*
b73
C
mo17


269
Amplicon437962
43224
206
206
IND
*
b73
G
mo17


269
Amplicon437962
43225
208
208
SNP
A
b73
C
mo17


269
Amplicon437962
43226
209
209
SNP
C
mo17
G
b73


269
Amplicon437962
43227
220
220
SNP
A
mo17
G
b73


269
Amplicon437962
43228
452
455
IND
****
mo17
ATAC
b73


269
Amplicon437962
43229
533
533
SNP
C
b73
T
mo17


269
Amplicon437962
43230
649
649
SNP
C
b73
G
mo17


270
Amplicon438229
43576
47
47
SNP
A
b73
T
mo17


270
Amplicon438229
43577
48
48
SNP
A
b73
T
mo17


270
Amplicon438229
43578
71
71
SNP
A
mo17
T
b73


270
Amplicon438229
43579
153
153
SNP
C
b73
T
mo17


270
Amplicon438229
43580
217
217
SNP
C
b73
T
mo17


270
Amplicon438229
43581
274
274
SNP
A
mo17
C
b73


271
Amplicon438421
43789
109
109
SNP
C
b73
T
mo17


272
Amplicon557338
48404
106
106
SNP
C
mo17
G
b73


272
Amplicon557338
48405
137
137
SNP
A
b73
C
mo17


272
Amplicon557338
48406
142
142
SNP
G
b73
T
mo17


272
Amplicon557338
48407
175
175
SNP
C
b73
T
mo17


272
Amplicon557338
48408
192
192
SNP
C
mo17
T
b73


272
Amplicon557338
48409
193
193
SNP
C
b73
T
mo17


272
Amplicon557338
48410
203
207
IND
*****
mo17
TGAAT
b73


272
Amplicon557338
48412
215
215
SNP
A
b73
C
mo17


272
Amplicon557338
48413
223
228
IND
******
mo17
TATCCA
b73


272
Amplicon557338
48414
256
256
SNP
A
mo17
T
b73


272
Amplicon557338
48415
266
266
SNP
C
b73
T
mo17


272
Amplicon557338
48416
317
317
SNP
A
mo17
C
b73


272
Amplicon557338
48417
356
356
SNP
A
mo17
G
b73


272
Amplicon557338
48418
365
365
SNP
A
b73
T
mo17


272
Amplicon557338
48419
366
366
IND
*
b73
C
mo17


272
Amplicon557338
48420
370
370
SNP
C
b73
T
mo17


272
Amplicon557338
48421
401
401
SNP
G
mo17
T
b73


272
Amplicon557338
48423
407
408
IND
**
mo17
TT
b73


272
Amplicon557338
48424
417
417
SNP
C
mo17
G
b73


272
Amplicon557338
48425
487
487
SNP
C
mo17
T
b73


272
Amplicon557338
48426
491
491
IND
*
mo17
C
b73


272
Amplicon557338
48427
528
531
IND
****
mo17
CAGC
b73


273
Amplicon557363
48561
27
27
SNP
C
b73
T
mo17


273
Amplicon557363
48562
216
216
SNP
G
mo17
T
b73


274
Amplicon557374
48615
33
33
SNP
C
mo17
T
b73


274
Amplicon557374
48616
87
87
SNP
C
mo17
T
b73


275
Amplicon557514
49292
161
161
SNP
C
mo17
T
b73


275
Amplicon557514
49293
182
182
SNP
A
mo17
C
b73


275
Amplicon557514
49294
185
185
SNP
A
b73
G
mo17


275
Amplicon557514
49295
222
222
SNP
A
b73
G
mo17


275
Amplicon557514
49296
223
223
SNP
A
b73
G
mo17


275
Amplicon557514
49297
242
242
SNP
G
b73
T
mo17


275
Amplicon557514
49298
295
295
SNP
C
mo17
T
b73


275
Amplicon557514
49299
371
371
SNP
C
b73
G
mo17


275
Amplicon557514
49301
448
448
SNP
C
mo17
T
b73


276
Amplicon557572
49557
463
463
SNP
A
mo17
G
b73


277
Amplicon557793
50315
255
255
SNP
A
mo17
G
b73


277
Amplicon557793
50316
355
355
SNP
A
mo17
G
b73


278
Amplicon558092
51399
37
43
IND
*******
mo17
TCAGTGG
b73


278
Amplicon558092
51400
178
178
SNP
A
mo17
T
b73


278
Amplicon558092
51401
179
179
SNP
A
mo17
C
b73


278
Amplicon558092
51402
234
234
SNP
A
mo17
C
b73


278
Amplicon558092
51403
278
278
SNP
A
mo17
C
b73


278
Amplicon558092
51404
284
284
SNP
C
mo17
T
b73


278
Amplicon558092
51405
327
327
SNP
C
mo17
T
b73


279
Amplicon558095
51419
251
251
SNP
C
b73
T
mo17


280
Amplicon558139
51609
45
45
SNP
A
b73
T
mo17


280
Amplicon558139
51610
196
196
SNP
C
b73
G
mo17


280
Amplicon558139
51611
200
200
SNP
C
mo17
T
b73


280
Amplicon558139
51613
270
270
SNP
A
mo17
G
b73


280
Amplicon558139
51614
319
319
SNP
C
b73
T
mo17


281
Amplicon558289
52078
104
104
IND
*
mo17
G
b73


281
Amplicon558289
52080
106
106
IND
*
mo17
C
b73


281
Amplicon558289
52081
350
350
SNP
C
b73
T
mo17


282
Amplicon558536
53097
334
334
SNP
A
mo17
T
b73


283
Amplicon558731
53899
472
473
IND
**
mo17
TA
b73


284
Amplicon558865
54409
76
76
SNP
C
mo17
T
b73


284
Amplicon558865
54410
100
100
SNP
C
mo17
T
b73


284
Amplicon558865
54411
110
110
SNP
A
mo17
G
b73


284
Amplicon558865
54412
444
444
SNP
A
mo17
T
b73


284
Amplicon558865
54413
478
478
SNP
A
b73
G
mo17


285
Amplicon558883
54460
410
410
SNP
A
b73
T
mo17


286
Amplicon559082
55370
254
254
SNP
C
b73
T
mo17


286
Amplicon559082
55371
354
354
SNP
C
mo17
T
b73


286
Amplicon559082
55372
355
355
SNP
G
b73
T
mo17


286
Amplicon559082
55373
418
418
IND
*
mo17
C
b73


286
Amplicon559082
55374
490
490
SNP
A
mo17
C
b73


287
Amplicon559441
56934
179
179
SNP
G
mo17
T
b73


287
Amplicon559441
56935
212
212
SNP
C
mo17
T
b73


287
Amplicon559441
56936
240
240
SNP
C
mo17
T
b73


287
Amplicon559441
56937
258
258
SNP
G
mo17
T
b73


287
Amplicon559441
56938
383
383
SNP
C
b73
T
mo17


287
Amplicon559441
56939
425
425
SNP
A
b73
C
mo17


288
Amplicon559491
57136
129
129
SNP
G
b73
T
mo17


288
Amplicon559491
57137
540
540
SNP
A
b73
G
mo17


289
Amplicon559625
57758
45
45
SNP
A
b73
G
mo17


290
Amplicon559759
58375
493
493
SNP
C
mo17
T
b73


291
Amplicon559897
58904
119
119
SNP
C
b73
G
mo17


291
Amplicon559897
58905
215
215
SNP
A
mo17
T
b73


291
Amplicon559897
58906
313
313
SNP
A
b73
T
mo17


292
Amplicon559922
59006
21
21
SNP
A
b73
T
mo17


292
Amplicon559922
59007
33
33
SNP
C
b73
G
mo17


292
Amplicon559922
59008
82
82
SNP
C
mo17
T
b73


292
Amplicon559922
59009
183
183
SNP
A
b73
C
mo17


292
Amplicon559922
59010
233
233
SNP
G
b73
T
mo17


292
Amplicon559922
59011
260
260
SNP
C
mo17
T
b73


293
Amplicon560273
60430
62
62
SNP
A
mo17
G
b73


293
Amplicon560273
60431
81
81
IND
*
mo17
T
b73


293
Amplicon560273
60433
143
143
SNP
A
mo17
C
b73


293
Amplicon560273
60434
165
165
IND
*
b73
T
mo17


294
Amplicon560371
60751
298
298
SNP
A
mo17
G
b73


294
Amplicon560371
60753
370
370
SNP
A
mo17
T
b73


294
Amplicon560371
60754
375
375
SNP
A
b73
C
mo17


294
Amplicon560371
60755
444
444
SNP
A
mo17
G
b73


295
Amplicon617328
68149
297
297
SNP
C
b73
T
mo17


295
Amplicon617328
68150
598
598
SNP
C
mo17
G
b73


296
Amplicon617436
68421
153
153
SNP
C
b73
T
mo17


296
Amplicon617436
68422
180
180
SNP
C
b73
T
mo17


296
Amplicon617436
68423
252
252
SNP
A
b73
G
mo17


296
Amplicon617436
68424
304
304
SNP
C
b73
T
mo17


296
Amplicon617436
68425
363
363
SNP
A
mo17
G
b73


296
Amplicon617436
68426
434
434
SNP
C
mo17
T
b73


296
Amplicon617436
68427
459
459
SNP
C
mo17
T
b73


297
Amplicon617439
68435
54
54
SNP
A
mo17
G
b73


297
Amplicon617439
68436
80
80
SNP
A
mo17
C
b73


297
Amplicon617439
68437
93
93
SNP
A
mo17
G
b73


297
Amplicon617439
68438
123
123
SNP
C
b73
T
mo17


297
Amplicon617439
68439
161
161
SNP
C
b73
T
mo17


297
Amplicon617439
68440
536
536
SNP
C
b73
G
mo17


298
Amplicon617662
68940
113
113
SNP
C
b73
T
mo17


298
Amplicon617662
68941
277
277
SNP
C
mo17
G
b73


298
Amplicon617662
68942
393
397
IND
*****
b73
ACGCT
mo17


298
Amplicon617662
68943
435
435
IND
*
mo17
C
b73


298
Amplicon617662
68944
436
445
IND
**********
mo17
CTTCTAAGAC
b73


299
Amplicon617671
68950
126
126
SNP
G
b73
T
mo17


299
Amplicon617671
68952
172
172
SNP
A
b73
G
mo17


299
Amplicon617671
68953
182
182
SNP
C
b73
T
mo17


299
Amplicon617671
68954
214
214
SNP
G
mo17
T
b73


299
Amplicon617671
68955
260
260
SNP
C
b73
T
mo17


299
Amplicon617671
68956
282
282
SNP
C
b73
T
mo17


299
Amplicon617671
68957
285
285
SNP
C
mo17
G
b73


299
Amplicon617671
68958
287
287
SNP
C
b73
T
mo17


299
Amplicon617671
68959
300
300
SNP
C
mo17
T
b73


299
Amplicon617671
68960
327
327
SNP
C
mo17
T
b73


299
Amplicon617671
68961
414
414
SNP
C
mo17
G
b73


299
Amplicon617671
68962
435
435
SNP
A
b73
G
mo17


300
Amplicon617706
69119
200
200
SNP
C
mo17
T
b73


300
Amplicon617706
69120
471
471
SNP
C
mo17
G
b73


300
Amplicon617706
69121
684
684
SNP
A
mo17
G
b73


300
Amplicon617706
69122
743
743
SNP
C
mo17
T
b73


300
Amplicon617706
69123
749
749
IND
*
mo17
G
b73


300
Amplicon617706
69125
754
754
IND
*
mo17
A
b73


301
Amplicon617780
69188
171
171
SNP
A
mo17
G
b73


302
Amplicon617951
69556
98
98
IND
*
b73
T
mo17


302
Amplicon617951
69557
100
100
SNP
A
mo17
G
b73


302
Amplicon617951
69558
179
179
SNP
G
b73
T
mo17


302
Amplicon617951
69559
202
202
SNP
C
mo17
T
b73


302
Amplicon617951
69562
240
240
SNP
C
b73
T
mo17


302
Amplicon617951
69563
261
261
SNP
A
mo17
G
b73


302
Amplicon617951
69565
590
590
SNP
A
mo17
G
b73


303
Amplicon617978
69588
244
244
SNP
C
mo17
T
b73


303
Amplicon617978
69589
264
264
SNP
G
b73
T
mo17


303
Amplicon617978
69590
282
282
SNP
C
b73
T
mo17


303
Amplicon617978
69591
349
349
SNP
C
mo17
T
b73


303
Amplicon617978
69592
438
439
IND
**
b73
AT
mo17


303
Amplicon617978
69593
540
540
SNP
A
mo17
C
b73


303
Amplicon617978
69594
541
541
SNP
A
mo17
T
b73


303
Amplicon617978
69595
585
585
SNP
A
b73
G
mo17


304
Amplicon617990
69630
598
598
SNP
A
b73
G
mo17


305
Amplicon645093
70727
140
140
SNP
C
b73
T
mo17


305
Amplicon645093
70728
178
178
SNP
C
b73
T
mo17


305
Amplicon645093
70730
330
330
SNP
C
mo17
G
b73


305
Amplicon645093
70731
944
944
SNP
A
mo17
C
b73


305
Amplicon645093
70732
979
979
SNP
A
b73
G
mo17


305
Amplicon645093
70733
1060
1060
SNP
A
b73
T
mo17


305
Amplicon645093
70734
1095
1095
SNP
C
b73
G
mo17


306
Amplicon645262
71154
300
302
IND
***
b73
TGA
mo17


306
Amplicon645262
71156
400
400
SNP
A
mo17
C
b73


306
Amplicon645262
71157
621
626
IND
******
b73
ATACTT
mo17


306
Amplicon645262
71158
779
779
SNP
G
mo17
T
b73


306
Amplicon645262
71159
902
903
IND
**
mo17
TG
b73


307
Amplicon645495
71622
51
51
SNP
A
mo17
G
b73


307
Amplicon645495
71623
52
52
SNP
A
mo17
G
b73


307
Amplicon645495
71624
105
105
SNP
A
b73
G
mo17


307
Amplicon645495
71625
127
127
SNP
A
b73
C
mo17


307
Amplicon645495
71627
138
140
IND
***
mo17
AAA
b73


307
Amplicon645495
71628
219
219
SNP
C
b73
T
mo17


307
Amplicon645495
71629
348
348
SNP
A
mo17
G
b73


307
Amplicon645495
71630
551
551
SNP
A
mo17
G
b73


307
Amplicon645495
71633
784
784
SNP
G
mo17
T
b73


307
Amplicon645495
71634
794
794
SNP
A
b73
G
mo17


307
Amplicon645495
71635
824
824
SNP
C
mo17
T
b73


307
Amplicon645495
71639
931
931
SNP
C
mo17
T
b73


307
Amplicon645495
71640
975
975
SNP
A
b73
C
mo17


308
Amplicon670815
76792
194
194
SNP
A
b73
G
mo17


308
Amplicon670815
76793
291
291
SNP
C
b73
T
mo17


309
Amplicon670910
77118
187
187
SNP
A
mo17
G
b73


310
Amplicon670989
77412
88
88
SNP
G
mo17
T
b73


310
Amplicon670989
77413
118
118
SNP
G
mo17
T
b73


310
Amplicon670989
77414
245
245
SNP
C
b73
T
mo17


311
Amplicon671032
77545
66
66
SNP
A
b73
G
mo17


312
Amplicon671043
77568
249
249
SNP
A
mo17
G
b73


313
Amplicon671315
78437
94
94
SNP
C
mo17
G
b73


313
Amplicon671315
78438
137
137
SNP
C
b73
T
mo17


314
Amplicon700255
78827
122
122
SNP
A
b73
G
mo17


314
Amplicon700255
78828
209
209
SNP
C
b73
T
mo17


314
Amplicon700255
78829
262
266
IND
*****
b73
GTGTG
mo17


314
Amplicon700255
78830
292
292
IND
*
b73
T
mo17


315
Amplicon700287
79073
97
97
SNP
A
mo17
G
b73


316
Amplicon700291
79081
79
79
SNP
A
mo17
C
b73


316
Amplicon700291
79082
164
164
SNP
A
b73
G
mo17


317
Amplicon700342
79300
202
202
SNP
C
b73
T
mo17


317
Amplicon700342
79301
223
223
SNP
C
b73
T
mo17


317
Amplicon700342
79303
250
250
SNP
A
b73
T
mo17


317
Amplicon700342
79304
252
252
SNP
C
mo17
G
b73


317
Amplicon700342
79305
297
297
SNP
A
b73
T
mo17


317
Amplicon700342
79306
321
321
SNP
A
mo17
G
b73


317
Amplicon700342
79307
373
373
IND
*
mo17
T
b73


318
Amplicon700384
79514
80
80
SNP
A
b73
G
mo17


318
Amplicon700384
79515
255
255
IND
*
b73
T
mo17


318
Amplicon700384
79516
307
307
SNP
A
b73
G
mo17


318
Amplicon700384
79517
321
321
SNP
A
b73
G
mo17


318
Amplicon700384
79518
448
448
SNP
A
mo17
G
b73


318
Amplicon700384
79519
480
480
SNP
C
b73
T
mo17


319
Amplicon700387
79529
130
130
SNP
A
mo17
T
b73


320
Amplicon700428
79693
127
127
SNP
C
mo17
T
b73


320
Amplicon700428
79694
159
159
SNP
C
b73
T
mo17


320
Amplicon700428
79695
204
204
SNP
G
b73
T
mo17


320
Amplicon700428
79696
220
220
IND
*
b73
T
mo17


320
Amplicon700428
79697
253
253
SNP
C
mo17
T
b73


320
Amplicon700428
79698
255
255
SNP
A
b73
C
mo17


320
Amplicon700428
79701
456
456
SNP
A
mo17
G
b73


320
Amplicon700428
79702
464
464
SNP
A
mo17
T
b73


321
Amplicon700512
80029
50
50
SNP
C
b73
T
mo17


321
Amplicon700512
80030
86
86
SNP
A
b73
T
mo17


321
Amplicon700512
80031
163
163
SNP
A
b73
G
mo17


321
Amplicon700512
80032
260
260
SNP
A
mo17
G
b73


321
Amplicon700512
80033
269
269
SNP
C
b73
T
mo17


321
Amplicon700512
80034
419
419
SNP
C
b73
T
mo17


321
Amplicon700512
80035
458
458
SNP
A
b73
G
mo17


322
Amplicon700579
80475
229
229
SNP
A
b73
G
mo17


323
Amplicon700609
80704
102
102
SNP
C
mo17
T
b73


323
Amplicon700609
80705
281
281
SNP
C
mo17
G
b73


324
Amplicon700771
81460
110
115
IND
******
mo17
GACGTA
b73


324
Amplicon700771
81461
129
129
SNP
C
b73
T
mo17


324
Amplicon700771
81462
157
157
SNP
C
mo17
G
b73


324
Amplicon700771
81463
195
195
IND
*
mo17
G
b73


324
Amplicon700771
81464
203
203
IND
*
mo17
T
b73


324
Amplicon700771
81466
207
207
IND
*
mo17
A
b73


324
Amplicon700771
81467
260
260
IND
*
b73
T
mo17


324
Amplicon700771
81469
264
265
IND
**
b73
GG
mo17


324
Amplicon700771
81470
266
266
SNP
G
mo17
T
b73


324
Amplicon700771
81471
315
318
IND
****
b73
GGAG
mo17


325
Amplicon724218
82235
506
506
SNP
A
mo17
C
b73


326
Amplicon724245
82295
252
252
SNP
C
mo17
T
b73


326
Amplicon724245
82296
508
508
SNP
A
b73
G
mo17


327
Amplicon724279
82454
156
156
SNP
A
mo17
C
b73


327
Amplicon724279
82455
203
203
SNP
C
b73
G
mo17


327
Amplicon724279
82456
280
280
SNP
A
mo17
G
b73


327
Amplicon724279
82457
282
282
SNP
C
b73
T
mo17


327
Amplicon724279
82458
384
384
SNP
G
mo17
T
b73


327
Amplicon724279
82460
474
474
SNP
A
mo17
G
b73


327
Amplicon724279
82461
481
481
SNP
G
b73
T
mo17


327
Amplicon724279
82462
525
525
SNP
A
mo17
T
b73


327
Amplicon724279
82463
541
541
SNP
G
mo17
T
b73


328
Amplicon724510
83776
319
319
SNP
A
b73
G
mo17


328
Amplicon724510
83777
336
336
SNP
C
b73
T
mo17


328
Amplicon724510
83778
371
371
SNP
C
mo17
T
b73


328
Amplicon724510
83779
374
374
SNP
G
mo17
T
b73


328
Amplicon724510
83780
398
398
SNP
G
mo17
T
b73


329
Amplicon724623
84517
231
231
SNP
A
mo17
G
b73


329
Amplicon724623
84521
299
299
SNP
G
mo17
T
b73


329
Amplicon724623
84527
546
546
SNP
A
mo17
C
b73


329
Amplicon724623
84529
556
556
SNP
C
mo17
G
b73


329
Amplicon724623
84530
565
566
IND
**
mo17
TG
b73


330
Amplicon740235
84829
505
505
SNP
A
b73
C
mo17


331
Amplicon993221
104389
210
210
SNP
C
LH82
T
5CM1


331
Amplicon993221
104390
224
224
SNP
C
LH82
G
5CM1


331
Amplicon993221
104391
225
225
SNP
A
LH82
G
5CM1


331
Amplicon993221
104392
226
226
SNP
C
5CM1
T
LH82


331
Amplicon993221
104393
230
230
SNP
C
5CM1
T
LH82


331
Amplicon993221
104394
232
232
SNP
A
5CM1
G
LH82


331
Amplicon993221
104395
251
251
SNP
C
LH82
G
5CM1


332
Amplicon993232
104447
306
306
SNP
A
LH82
C
5CM1


333
Amplicon993240
104473
221
221
SNP
A
5CM1
C
LH82


333
Amplicon993240
104474
260
260
SNP
C
5CM1
T
LH82


333
Amplicon993240
104475
305
305
SNP
A
5CM1
C
LH82


333
Amplicon993240
104476
316
316
SNP
A
LH82
G
5CM1


334
Amplicon993248
104509
287
293
IND
*******
LH82
CCAACAA
5CM1


334
Amplicon993248
104510
365
365
SNP
C
LH82
G
5CM1


335
Amplicon993299
104672
124
124
SNP
A
LH82
C
5CM1


336
Amplicon993328
104809
22
22
SNP
C
LH82
T
5CM1


336
Amplicon993328
104810
23
23
SNP
G
5CM1
T
LH82


336
Amplicon993328
104811
24
24
SNP
A
5CM1
G
LH82


336
Amplicon993328
104812
25
25
SNP
C
LH82
T
5CM1


336
Amplicon993328
104813
26
26
SNP
C
LH82
T
5CM1


336
Amplicon993328
104814
27
27
SNP
A
LH82
C
5CM1


336
Amplicon993328
104815
28
28
SNP
C
5CM1
G
LH82


336
Amplicon993328
104816
29
29
SNP
A
LH82
G
5CM1


336
Amplicon993328
104817
30
30
SNP
A
5CM1
G
LH82


336
Amplicon993328
104818
31
31
SNP
A
LH82
T
5CM1


336
Amplicon993328
104819
33
33
SNP
A
5CM1
C
LH82


336
Amplicon993328
104820
34
34
SNP
A
LH82
C
5CM1


336
Amplicon993328
104821
35
35
SNP
A
LH82
T
5CM1


336
Amplicon993328
104822
45
45
SNP
A
5CM1
C
LH82


336
Amplicon993328
104823
96
96
SNP
A
LH82
C
5CM1


336
Amplicon993328
104824
97
99
IND
***
5CM1
AAA
LH82


336
Amplicon993328
104825
183
183
SNP
C
LH82
T
5CM1


336
Amplicon993328
104826
212
212
SNP
C
5CM1
T
LH82


336
Amplicon993328
104827
275
275
SNP
A
5CM1
G
LH82


336
Amplicon993328
104828
474
474
SNP
C
5CM1
T
LH82


337
Amplicon993333
104845
32
32
SNP
A
5CM1
G
LH82


337
Amplicon993333
104846
40
40
SNP
C
5CM1
T
LH82


337
Amplicon993333
104847
141
141
SNP
A
5CM1
T
LH82


337
Amplicon993333
104848
323
323
SNP
A
LH82
C
5CM1


337
Amplicon993333
104849
365
365
SNP
G
LH82
T
5CM1


337
Amplicon993333
104850
399
399
SNP
A
5CM1
C
LH82


337
Amplicon993333
104851
431
431
SNP
G
LH82
T
5CM1


337
Amplicon993333
104852
434
434
SNP
C
5CM1
T
LH82


337
Amplicon993333
104853
455
455
SNP
A
LH82
T
5CM1


337
Amplicon993333
104854
456
456
SNP
A
LH82
T
5CM1


337
Amplicon993333
104855
460
460
IND
*
LH82
C
5CM1


338
Amplicon993334
104857
84
84
SNP
C
LH82
T
5CM1


338
Amplicon993334
104858
172
174
IND
***
LH82
GCT
5CM1


338
Amplicon993334
104859
209
209
SNP
C
LH82
T
5CM1


338
Amplicon993334
104860
417
417
SNP
C
LH82
G
5CM1


339
Amplicon993335
104861
224
224
SNP
G
5CM1
T
LH82


339
Amplicon993335
104862
320
320
SNP
C
5CM1
T
LH82


340
Amplicon993348
104939
36
36
SNP
A
LH82
G
5CM1


340
Amplicon993348
104940
46
46
SNP
C
5CM1
T
LH82


340
Amplicon993348
104941
53
53
SNP
C
LH82
G
5CM1


340
Amplicon993348
104942
60
60
SNP
A
5CM1
G
LH82


340
Amplicon993348
104943
64
64
SNP
A
5CM1
G
LH82


340
Amplicon993348
104944
81
90
IND
**********
5CM1
CATTTTCAGT
LH82


340
Amplicon993348
104945
111
111
SNP
G
5CM1
T
LH82


340
Amplicon993348
104946
268
268
SNP
A
5CM1
T
LH82


340
Amplicon993348
104947
273
273
SNP
A
LH82
G
5CM1


340
Amplicon993348
104948
295
295
SNP
C
LH82
T
5CM1


340
Amplicon993348
104949
394
394
SNP
A
LH82
G
5CM1


341
Amplicon993350
104950
76
76
SNP
A
5CM1
G
LH82


341
Amplicon993350
104951
126
126
SNP
G
5CM1
T
LH82


341
Amplicon993350
104952
162
162
SNP
A
LH82
T
5CM1


341
Amplicon993350
104953
314
314
SNP
A
5CM1
G
LH82


341
Amplicon993350
104954
375
375
SNP
G
5CM1
T
LH82


342
Amplicon993378
105008
68
71
IND
****
LH82
GTGC
5CM1


342
Amplicon993378
105009
97
97
SNP
A
5CM1
G
LH82


342
Amplicon993378
105010
113
113
SNP
A
5CM1
G
LH82


342
Amplicon993378
105011
116
116
SNP
C
LH82
T
5CM1


342
Amplicon993378
105012
270
270
SNP
A
5CM1
G
LH82


342
Amplicon993378
105013
273
273
SNP
C
5CM1
T
LH82


342
Amplicon993378
105014
347
347
SNP
C
5CM1
G
LH82


342
Amplicon993378
105015
428
428
SNP
C
LH82
T
5CM1


342
Amplicon993378
105016
430
430
SNP
C
LH82
G
5CM1


342
Amplicon993378
105017
436
436
SNP
A
5CM1
T
LH82


342
Amplicon993378
105018
438
438
SNP
C
LH82
T
5CM1


342
Amplicon993378
105019
439
439
SNP
G
LH82
T
5CM1


343
Amplicon993485
105546
103
105
IND
***
5CM1
AGC
LH82


343
Amplicon993485
105547
163
168
IND
******
LH82
GACTGC
5CM1


343
Amplicon993485
105548
221
221
SNP
A
5CM1
C
LH82


343
Amplicon993485
105549
229
229
SNP
C
LH82
G
5CM1


344
Amplicon993496
105584
79
80
IND
**
5CM1
CT
LH82


344
Amplicon993496
105586
173
173
SNP
C
5CM1
G
LH82


344
Amplicon993496
105587
376
376
SNP
A
5CM1
G
LH82


344
Amplicon993496
105588
382
382
SNP
G
5CM1
T
LH82


344
Amplicon993496
105589
469
469
SNP
A
5CM1
T
LH82


344
Amplicon993496
105590
474
474
SNP
C
LH82
T
5CM1


344
Amplicon993496
105591
477
477
SNP
G
LH82
T
5CM1


344
Amplicon993496
105592
523
523
SNP
C
5CM1
T
LH82


344
Amplicon993496
105593
536
538
IND
***
LH82
CGT
5CM1


345
Amplicon993505
105613
177
177
SNP
C
5CM1
G
LH82


346
Amplicon993513
105647
198
198
SNP
G
LH82
T
5CM1


346
Amplicon993513
105648
275
275
SNP
C
5CM1
T
LH82


347
Amplicon993561
105854
346
346
SNP
A
5CM1
G
LH82


347
Amplicon993561
105855
481
481
SNP
A
5CM1
C
LH82


348
Amplicon993642
106239
72
72
SNP
C
LH82
T
5CM1


348
Amplicon993642
106240
117
117
IND
*
5CM1
T
LH82


348
Amplicon993642
106241
142
142
SNP
A
LH82
G
5CM1


348
Amplicon993642
106242
153
154
IND
**
LH82
TT
5CM1


348
Amplicon993642
106243
206
206
SNP
G
5CM1
T
LH82


348
Amplicon993642
106244
311
311
SNP
A
5CM1
G
LH82


348
Amplicon993642
106245
361
361
SNP
C
5CM1
T
LH82


349
Amplicon993659
106297
237
238
IND
**
LH82
GT
5CM1


349
Amplicon993659
106298
292
296
IND
*****
LH82
ATAAT
5CM1


349
Amplicon993659
106299
310
310
SNP
G
5CM1
T
LH82


349
Amplicon993659
106300
328
329
IND
**
5CM1
CT
LH82


349
Amplicon993659
106301
420
420
SNP
C
LH82
T
5CM1


349
Amplicon993659
106302
424
424
SNP
A
5CM1
G
LH82


349
Amplicon993659
106303
432
434
IND
***
LH82
CGG
5CM1


349
Amplicon993659
106304
455
455
SNP
G
LH82
T
5CM1


350
Amplicon993704
106482
38
38
SNP
A
5CM1
C
LH82


350
Amplicon993704
106483
80
80
IND
*
LH82
A
5CM1


350
Amplicon993704
106484
89
89
SNP
A
5CM1
C
LH82


350
Amplicon993704
106485
91
91
SNP
C
LH82
T
5CM1


350
Amplicon993704
106486
109
109
SNP
A
5CM1
G
LH82


350
Amplicon993704
106487
126
126
SNP
A
LH82
G
5CM1


350
Amplicon993704
106488
235
235
SNP
C
5CM1
G
LH82


350
Amplicon993704
106489
354
354
SNP
A
5CM1
G
LH82


350
Amplicon993704
106490
387
387
SNP
A
5CM1
G
LH82


350
Amplicon993704
106491
393
393
SNP
C
LH82
T
5CM1


351
Amplicon993739
106669
152
152
SNP
C
5CM1
T
LH82


351
Amplicon993739
106670
172
172
SNP
C
LH82
G
5CM1


351
Amplicon993739
106671
182
182
SNP
A
5CM1
T
LH82


351
Amplicon993739
106672
183
183
SNP
A
5CM1
C
LH82


351
Amplicon993739
106673
216
219
IND
****
LH82
TATA
5CM1


351
Amplicon993739
106674
235
236
IND
**
5CM1
CT
LH82


351
Amplicon993739
106675
241
244
IND
****
5CM1
CTAT
LH82


351
Amplicon993739
106676
247
247
SNP
A
LH82
G
5CM1


351
Amplicon993739
106677
262
263
IND
**
LH82
AC
5CM1


351
Amplicon993739
106678
308
308
SNP
C
LH82
G
5CM1


351
Amplicon993739
106679
319
319
SNP
C
5CM1
T
LH82


351
Amplicon993739
106680
325
325
SNP
C
5CM1
T
LH82


351
Amplicon993739
106681
355
355
SNP
C
5CM1
T
LH82


352
Amplicon993761
106742
107
107
SNP
A
5CM1
G
LH82


352
Amplicon993761
106743
234
234
SNP
A
5CM1
G
LH82


352
Amplicon993761
106744
273
273
SNP
A
5CM1
G
LH82


352
Amplicon993761
106745
501
501
SNP
C
5CM1
T
LH82


353
Amplicon993787
106842
388
388
SNP
A
5CM1
G
LH82


354
Amplicon993789
106844
81
81
SNP
A
5CM1
G
LH82


354
Amplicon993789
106845
109
109
SNP
A
LH82
G
5CM1


355
Amplicon993806
106912
182
182
SNP
C
LH82
G
5CM1


356
Amplicon993827
107061
238
244
IND
*******
5CM1
GTATGAA
LH82


357
Amplicon993841
107074
180
180
SNP
C
5CM1
T
LH82


357
Amplicon993841
107075
194
194
SNP
C
5CM1
T
LH82


357
Amplicon993841
107076
205
205
SNP
C
LH82
T
5CM1


357
Amplicon993841
107077
380
380
SNP
A
LH82
G
5CM1


357
Amplicon993841
107078
431
431
SNP
C
LH82
T
5CM1


358
Amplicon993884
107274
79
79
SNP
C
LH82
G
5CM1


358
Amplicon993884
107275
289
289
SNP
C
5CM1
T
LH82


358
Amplicon993884
107276
401
401
SNP
A
LH82
G
5CM1


358
Amplicon993884
107277
486
486
IND
*
5CM1
G
LH82


358
Amplicon993884
107278
487
489
IND
***
5CM1
GTG
LH82


359
Amplicon993886
107279
159
159
SNP
A
5CM1
G
LH82


359
Amplicon993886
107280
165
165
SNP
A
LH82
G
5CM1


359
Amplicon993886
107285
211
211
SNP
A
5CM1
G
LH82


359
Amplicon993886
107286
256
256
SNP
C
LH82
T
5CM1


360
Amplicon993907
107396
74
76
IND
***
LH82
AGC
5CM1


360
Amplicon993907
107397
90
90
SNP
C
LH82
G
5CM1


360
Amplicon993907
107398
213
213
SNP
A
5CM1
T
LH82


361
Amplicon993920
107449
284
284
SNP
C
LH82
T
5CM1


362
Amplicon993953
107617
110
110
SNP
C
5CM1
T
LH82


362
Amplicon993953
107618
125
125
SNP
C
LH82
T
5CM1


362
Amplicon993953
107619
127
127
SNP
G
5CM1
T
LH82


362
Amplicon993953
107620
347
347
SNP
A
5CM1
G
LH82


362
Amplicon993953
107621
365
371
IND
*******
LH82
GTTACAA
5CM1


363
Amplicon993958
107639
206
206
SNP
A
5CM1
T
LH82


364
Amplicon993973
107693
86
86
SNP
C
LH82
T
5CM1


364
Amplicon993973
107694
155
155
SNP
C
5CM1
T
LH82


364
Amplicon993973
107695
160
160
SNP
A
LH82
G
5CM1


364
Amplicon993973
107696
180
188
IND
*********
LH82
AAATTTGGT
5CM1


364
Amplicon993973
107697
212
212
SNP
A
5CM1
G
LH82


364
Amplicon993973
107698
293
293
SNP
C
LH82
T
5CM1


364
Amplicon993973
107699
336
336
SNP
A
LH82
G
5CM1


364
Amplicon993973
107700
372
372
SNP
A
LH82
G
5CM1


364
Amplicon993973
107701
376
376
SNP
A
LH82
G
5CM1


364
Amplicon993973
107702
441
441
IND
*
5CM1
A
LH82


365
Amplicon994009
107784
96
96
SNP
G
LH82
T
5CM1


365
Amplicon994009
107785
117
117
SNP
C
LH82
T
5CM1


365
Amplicon994009
107786
166
166
SNP
A
5CM1
G
LH82


365
Amplicon994009
107787
174
174
SNP
A
LH82
C
5CM1


365
Amplicon994009
107788
537
537
SNP
A
5CM1
T
LH82


366
Amplicon994012
107839
34
34
IND
*
LH82
T
5CM1


366
Amplicon994012
107840
153
153
SNP
C
LH82
G
5CM1


366
Amplicon994012
107841
211
211
SNP
A
5CM1
C
LH82


366
Amplicon994012
107842
220
220
SNP
A
5CM1
G
LH82


366
Amplicon994012
107844
423
431
IND
*********
5CM1
TATTTTCGT
LH82


366
Amplicon994012
107845
447
447
SNP
G
LH82
T
5CM1


367
Amplicon994024
107857
186
188
IND
***
LH82
TAA
5CM1


367
Amplicon994024
107858
400
400
SNP
C
5CM1
T
LH82


367
Amplicon994024
107859
448
448
IND
*
LH82
A
5CM1


367
Amplicon994024
107860
474
474
SNP
C
5CM1
T
LH82


367
Amplicon994024
107861
478
478
SNP
A
LH82
G
5CM1


368
Amplicon994036
107908
92
92
SNP
G
5CM1
T
LH82


368
Amplicon994036
107909
151
151
SNP
A
5CM1
G
LH82


368
Amplicon994036
107910
319
319
SNP
A
5CM1
T
LH82


368
Amplicon994036
107911
384
384
SNP
G
LH82
T
5CM1


368
Amplicon994036
107912
442
442
SNP
C
LH82
T
5CM1


368
Amplicon994036
107913
473
473
SNP
C
LH82
T
5CM1


369
Amplicon994045
107937
310
310
SNP
A
5CM1
G
LH82


369
Amplicon994045
107938
331
331
SNP
C
5CM1
T
LH82


369
Amplicon994045
107939
339
339
SNP
G
LH82
T
5CM1


369
Amplicon994045
107940
415
415
SNP
A
5CM1
C
LH82


370
Amplicon994047
107941
365
365
SNP
A
LH82
G
5CM1


371
Amplicon994062
108008
100
100
SNP
A
LH82
G
5CM1


371
Amplicon994062
108009
133
133
SNP
C
LH82
T
5CM1


371
Amplicon994062
108010
153
153
IND
*
LH82
T
5CM1


371
Amplicon994062
108011
176
176
SNP
C
LH82
T
5CM1


371
Amplicon994062
108012
233
233
SNP
C
5CM1
T
LH82


371
Amplicon994062
108013
339
339
SNP
C
5CM1
T
LH82


372
Amplicon994124
108211
235
235
SNP
A
5CM1
G
LH82


372
Amplicon994124
108212
336
337
IND
**
LH82
GA
5CM1


373
Amplicon1017005
108432
59
59
SNP
C
LH82
T
5CM1


373
Amplicon1017005
108433
227
227
SNP
C
LH82
T
5CM1


373
Amplicon1017005
108434
240
240
SNP
A
5CM1
G
LH82


373
Amplicon1017005
108435
268
268
SNP
G
5CM1
T
LH82


373
Amplicon1017005
108436
382
382
SNP
C
5CM1
T
LH82


373
Amplicon1017005
108437
479
479
SNP
A
LH82
G
5CM1


373
Amplicon1017005
108438
502
502
SNP
A
5CM1
G
LH82


373
Amplicon1017005
108439
513
513
SNP
A
5CM1
T
LH82


373
Amplicon1017005
108440
514
514
SNP
A
5CM1
G
LH82


373
Amplicon1017005
108441
515
515
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108442
516
516
SNP
G
LH82
T
5CM1


373
Amplicon1017005
108443
518
518
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108444
519
519
SNP
C
LH82
T
5CM1


373
Amplicon1017005
108445
520
520
SNP
A
LH82
C
5CM1


373
Amplicon1017005
108446
521
521
SNP
C
5CM1
G
LH82


373
Amplicon1017005
108447
522
522
SNP
C
LH82
T
5CM1


373
Amplicon1017005
108448
523
523
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108449
524
524
SNP
C
LH82
G
5CM1


373
Amplicon1017005
108450
525
525
SNP
C
LH82
T
5CM1


373
Amplicon1017005
108451
526
526
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108452
527
527
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108453
530
530
SNP
A
5CM1
C
LH82


373
Amplicon1017005
108454
531
531
SNP
C
5CM1
T
LH82


373
Amplicon1017005
108455
532
532
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108456
533
533
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108457
534
534
SNP
A
5CM1
G
LH82


373
Amplicon1017005
108458
535
535
SNP
G
LH82
T
5CM1


373
Amplicon1017005
108459
536
536
SNP
G
LH82
T
5CM1


373
Amplicon1017005
108460
538
538
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108461
539
539
SNP
C
LH82
T
5CM1


373
Amplicon1017005
108462
540
540
SNP
G
LH82
T
5CM1


373
Amplicon1017005
108463
541
541
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108464
542
542
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108465
544
544
SNP
G
5CM1
T
LH82


373
Amplicon1017005
108466
546
546
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108467
547
547
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108468
549
549
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108469
551
551
SNP
A
5CM1
C
LH82


373
Amplicon1017005
108470
552
552
SNP
A
5CM1
C
LH82


373
Amplicon1017005
108471
553
553
SNP
A
5CM1
C
LH82


373
Amplicon1017005
108472
554
554
SNP
G
5CM1
T
LH82


373
Amplicon1017005
108473
556
556
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108474
557
557
SNP
A
LH82
G
5CM1


373
Amplicon1017005
108475
558
558
SNP
A
LH82
C
5CM1


373
Amplicon1017005
108476
559
559
SNP
A
LH82
C
5CM1


373
Amplicon1017005
108477
561
561
SNP
A
LH82
G
5CM1


373
Amplicon1017005
108478
562
562
SNP
A
5CM1
T
LH82


373
Amplicon1017005
108479
564
564
SNP
A
LH82
C
5CM1


373
Amplicon1017005
108480
565
565
SNP
A
5CM1
T
LH82


373
Amplicon1017005
108481
566
566
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108482
567
567
SNP
A
LH82
T
5CM1


373
Amplicon1017005
108483
568
568
SNP
A
5CM1
T
LH82


373
Amplicon1017005
108484
569
569
SNP
C
5CM1
T
LH82


373
Amplicon1017005
108486
571
571
SNP
C
5CM1
T
LH82


373
Amplicon1017005
108489
575
575
SNP
C
5CM1
T
LH82


374
Amplicon1017007
108492
126
126
SNP
C
LH82
T
5CM1


374
Amplicon1017007
108493
303
303
SNP
A
LH82
G
5CM1


374
Amplicon1017007
108494
356
356
SNP
G
5CM1
T
LH82


374
Amplicon1017007
108495
480
480
SNP
C
LH82
T
5CM1


375
Amplicon1017028
108630
340
340
SNP
A
5CM1
G
LH82


376
Amplicon1017085
108862
48
48
SNP
C
LH82
T
5CM1


376
Amplicon1017085
108863
111
111
SNP
C
5CM1
G
LH82


376
Amplicon1017085
108864
118
118
SNP
A
5CM1
T
LH82


376
Amplicon1017085
108865
188
188
SNP
C
LH82
G
5CM1


376
Amplicon1017085
108866
296
296
SNP
A
LH82
C
5CM1


376
Amplicon1017085
108867
298
298
SNP
G
5CM1
T
LH82


376
Amplicon1017085
108868
383
384
IND
**
LH82
AC
5CM1


376
Amplicon1017085
108869
388
388
SNP
A
5CM1
G
LH82


376
Amplicon1017085
108870
411
411
SNP
A
5CM1
G
LH82


376
Amplicon1017085
108871
435
435
SNP
A
5CM1
G
LH82


376
Amplicon1017085
108872
440
440
SNP
C
5CM1
T
LH82


376
Amplicon1017085
108873
496
497
IND
**
LH82
GA
5CM1


376
Amplicon1017085
108874
524
524
SNP
C
LH82
T
5CM1


376
Amplicon1017085
108875
582
582
SNP
A
LH82
T
5CM1


377
Amplicon1017165
109198
89
89
SNP
A
5CM1
C
LH82


377
Amplicon1017165
109199
90
90
IND
*
LH82
T
5CM1


377
Amplicon1017165
109200
94
95
IND
**
LH82
CC
5CM1


377
Amplicon1017165
109201
97
97
SNP
A
LH82
G
5CM1


377
Amplicon1017165
109202
99
99
SNP
C
LH82
T
5CM1


377
Amplicon1017165
109203
100
100
SNP
A
5CM1
C
LH82


377
Amplicon1017165
109204
102
102
SNP
C
5CM1
G
LH82


377
Amplicon1017165
109205
106
106
SNP
C
5CM1
G
LH82


377
Amplicon1017165
109206
107
107
SNP
A
LH82
G
5CM1


377
Amplicon1017165
109207
150
150
SNP
C
5CM1
T
LH82


377
Amplicon1017165
109208
159
159
SNP
A
5CM1
G
LH82


377
Amplicon1017165
109209
272
272
SNP
G
LH82
T
5CM1


377
Amplicon1017165
109210
299
299
SNP
G
5CM1
T
LH82


377
Amplicon1017165
109211
303
303
SNP
A
LH82
G
5CM1


377
Amplicon1017165
109212
477
477
SNP
A
5CM1
C
LH82


377
Amplicon1017165
109213
478
478
SNP
A
5CM1
G
LH82


377
Amplicon1017165
109214
479
479
SNP
A
5CM1
G
LH82


377
Amplicon1017165
109215
506
506
SNP
A
LH82
G
5CM1


377
Amplicon1017165
109216
530
530
SNP
G
5CM1
T
LH82


377
Amplicon1017165
109217
548
548
SNP
C
LH82
T
5CM1


377
Amplicon1017165
109218
575
575
SNP
C
5CM1
G
LH82


377
Amplicon1017165
109219
581
581
IND
*
5CM1
C
LH82


377
Amplicon1017165
109220
585
585
IND
*
5CM1
C
LH82


377
Amplicon1017165
109221
593
593
SNP
A
LH82
C
5CM1


377
Amplicon1017165
109222
595
595
SNP
A
LH82
C
5CM1


378
Amplicon1017176
109306
27
27
SNP
A
LH82
C
5CM1


378
Amplicon1017176
109307
70
70
SNP
A
LH82
G
5CM1


378
Amplicon1017176
109308
72
72
IND
*
5CM1
G
LH82


378
Amplicon1017176
109309
78
78
IND
*
5CM1
T
LH82


378
Amplicon1017176
109310
80
80
SNP
A
5CM1
G
LH82


378
Amplicon1017176
109311
127
127
SNP
C
LH82
T
5CM1


378
Amplicon1017176
109312
145
145
SNP
C
5CM1
T
LH82


378
Amplicon1017176
109313
175
176
IND
**
5CM1
AT
LH82


378
Amplicon1017176
109314
250
250
SNP
G
LH82
T
5CM1


378
Amplicon1017176
109315
268
268
SNP
C
5CM1
T
LH82


379
Amplicon1017183
109328
565
565
SNP
A
LH82
G
5CM1


380
Amplicon1017186
109342
525
525
SNP
A
LH82
G
5CM1


381
Amplicon1017193
109396
439
448
IND
**********
LH82
ACACACACAC
5CM1


381
Amplicon1017193
109397
481
481
SNP
C
LH82
G
5CM1


381
Amplicon1017193
109398
487
490
IND
****
LH82
CTCA
5CM1


381
Amplicon1017193
109399
495
495
SNP
C
LH82
G
5CM1


381
Amplicon1017193
109400
499
499
SNP
C
LH82
G
5CM1


381
Amplicon1017193
109401
503
503
SNP
C
LH82
G
5CM1


381
Amplicon1017193
109402
510
510
SNP
A
5CM1
G
LH82


381
Amplicon1017193
109403
522
524
IND
***
LH82
TTC
5CM1


381
Amplicon1017193
109404
539
539
SNP
C
LH82
G
5CM1


382
Amplicon1017219
109508
47
47
SNP
C
LH82
T
5CM1


382
Amplicon1017219
109509
53
53
SNP
A
5CM1
G
LH82


382
Amplicon1017219
109510
155
155
SNP
C
LH82
T
5CM1


382
Amplicon1017219
109511
179
179
SNP
G
LH82
T
5CM1


382
Amplicon1017219
109512
187
187
SNP
C
LH82
G
5CM1


382
Amplicon1017219
109513
204
204
SNP
C
LH82
G
5CM1


382
Amplicon1017219
109514
206
208
IND
***
5CM1
CAC
LH82


382
Amplicon1017219
109516
214
218
IND
*****
5CM1
TTCCT
LH82


383
Amplicon1017256
109666
74
74
SNP
C
LH82
G
5CM1


383
Amplicon1017256
109667
80
80
SNP
A
5CM1
G
LH82


383
Amplicon1017256
109668
267
267
SNP
A
5CM1
C
LH82


383
Amplicon1017256
109669
326
326
SNP
A
5CM1
G
LH82


383
Amplicon1017256
109670
355
355
SNP
C
LH82
T
5CM1


383
Amplicon1017256
109672
447
447
SNP
C
5CM1
T
LH82


383
Amplicon1017256
109673
476
476
IND
*
LH82
A
5CM1


383
Amplicon1017256
109674
477
478
IND
**
LH82
GA
5CM1


383
Amplicon1017256
109675
542
542
SNP
A
5CM1
G
LH82


384
Amplicon1017272
109720
108
108
SNP
A
LH82
G
5CM1


384
Amplicon1017272
109721
193
193
SNP
A
LH82
C
5CM1


384
Amplicon1017272
109722
560
560
SNP
A
5CM1
G
LH82


385
Amplicon1017287
109797
165
168
IND
****
LH82
TACG
5CM1


385
Amplicon1017287
109798
212
212
SNP
A
LH82
G
5CM1


385
Amplicon1017287
109799
215
215
SNP
A
LH82
G
5CM1


385
Amplicon1017287
109800
222
224
IND
***
LH82
TCT
5CM1


385
Amplicon1017287
109801
331
331
SNP
A
5CM1
C
LH82


385
Amplicon1017287
109802
516
516
SNP
C
5CM1
G
LH82


385
Amplicon1017287
109803
562
562
SNP
C
5CM1
T
LH82


385
Amplicon1017287
109804
576
577
IND
**
5CM1
AG
LH82


385
Amplicon1017287
109805
642
642
SNP
A
LH82
G
5CM1


386
Amplicon1017331
110063
16
16
SNP
G
5CM1
T
LH82


386
Amplicon1017331
110064
20
20
SNP
C
LH82
G
5CM1


386
Amplicon1017331
110065
122
122
SNP
A
5CM1
G
LH82


386
Amplicon1017331
110066
244
247
IND
****
LH82
TATA
5CM1


386
Amplicon1017331
110067
275
275
SNP
A
5CM1
G
LH82


386
Amplicon1017331
110068
280
280
SNP
C
5CM1
G
LH82


386
Amplicon1017331
110069
313
313
SNP
A
LH82
G
5CM1


386
Amplicon1017331
110070
374
374
SNP
G
5CM1
T
LH82


387
Amplicon1017357
110147
244
244
SNP
C
5CM1
T
LH82


387
Amplicon1017357
110148
253
253
SNP
C
LH82
T
5CM1


387
Amplicon1017357
110149
285
285
SNP
A
5CM1
G
LH82


387
Amplicon1017357
110150
380
386
IND
*******
5CM1
CAGGAAA
LH82


388
Amplicon1017409
110376
262
262
SNP
A
5CM1
G
LH82


388
Amplicon1017409
110377
550
550
SNP
C
LH82
T
5CM1


389
Amplicon1017456
110603
268
268
SNP
C
5CM1
T
LH82


389
Amplicon1017456
110604
276
276
IND
*
LH82
G
5CM1


389
Amplicon1017456
110605
303
303
SNP
A
LH82
G
5CM1


389
Amplicon1017456
110606
446
446
SNP
A
LH82
C
5CM1


389
Amplicon1017456
110607
483
483
SNP
G
LH82
T
5CM1


389
Amplicon1017456
110608
528
528
SNP
A
LH82
G
5CM1


390
Amplicon1017466
110684
269
269
SNP
C
LH82
T
5CM1


390
Amplicon1017466
110685
475
475
SNP
C
5CM1
T
LH82


391
Amplicon1017484
110769
431
433
IND
***
LH82
ACT
5CM1


391
Amplicon1017484
110770
434
434
IND
*
LH82
T
5CM1


391
Amplicon1017484
110771
489
489
SNP
A
LH82
C
5CM1


392
Amplicon1017493
110780
359
359
SNP
A
LH82
G
5CM1


393
Amplicon1017498
110792
17
17
SNP
A
LH82
C
5CM1


393
Amplicon1017498
110793
23
23
SNP
G
LH82
T
5CM1


393
Amplicon1017498
110795
47
48
IND
**
LH82
TA
5CM1


393
Amplicon1017498
110796
117
117
SNP
C
5CM1
T
LH82


393
Amplicon1017498
110797
170
170
SNP
G
5CM1
T
LH82


393
Amplicon1017498
110798
248
248
SNP
C
LH82
T
5CM1


393
Amplicon1017498
110799
282
282
SNP
C
5CM1
G
LH82


393
Amplicon1017498
110800
405
405
SNP
A
5CM1
G
LH82


393
Amplicon1017498
110801
411
411
SNP
C
5CM1
T
LH82


393
Amplicon1017498
110802
412
412
SNP
A
LH82
C
5CM1


393
Amplicon1017498
110803
567
567
SNP
A
LH82
G
5CM1


394
Amplicon1017519
110886
93
98
IND
******
LH82
ATCTGC
5CM1


394
Amplicon1017519
110887
135
135
SNP
C
LH82
T
5CM1


394
Amplicon1017519
110888
261
264
IND
****
LH82
TTAT
5CM1


394
Amplicon1017519
110889
355
355
SNP
G
LH82
T
5CM1


394
Amplicon1017519
110890
402
402
IND
*
LH82
T
5CM1


394
Amplicon1017519
110891
404
408
IND
*****
LH82
CCTGT
5CM1


394
Amplicon1017519
110892
431
431
SNP
A
5CM1
T
LH82


394
Amplicon1017519
110894
464
470
IND
*******
LH82
GAACCAA
5CM1


394
Amplicon1017519
110895
546
546
SNP
C
5CM1
G
LH82


394
Amplicon1017519
110896
552
552
IND
*
LH82
A
5CM1


394
Amplicon1017519
110897
554
556
IND
***
LH82
CAT
5CM1


395
Amplicon1017554
110972
53
53
SNP
A
5CM1
G
LH82


395
Amplicon1017554
110973
390
390
SNP
C
LH82
T
5CM1


396
Amplicon1049957
111004
181
181
SNP
A
LH82
G
5CM1


396
Amplicon1049957
111005
246
246
SNP
C
5CM1
T
LH82


396
Amplicon1049957
111006
268
268
SNP
C
5CM1
T
LH82


396
Amplicon1049957
111007
284
284
SNP
A
5CM1
G
LH82


396
Amplicon1049957
111008
349
349
SNP
C
LH82
T
5CM1


396
Amplicon1049957
111009
559
559
SNP
C
LH82
G
5CM1


397
Amplicon1049995
111204
254
254
SNP
A
LH82
G
5CM1


397
Amplicon1049995
111207
509
509
SNP
A
5CM1
G
LH82


398
Amplicon1049999
111212
165
165
SNP
A
5CM1
G
LH82


399
Amplicon1050057
111365
76
76
SNP
C
5CM1
T
LH82


399
Amplicon1050057
111366
139
139
SNP
C
LH82
G
5CM1


399
Amplicon1050057
111367
258
258
SNP
A
5CM1
G
LH82


399
Amplicon1050057
111368
600
600
SNP
C
5CM1
T
LH82


400
Amplicon1050089
111464
114
114
SNP
A
5CM1
G
LH82


400
Amplicon1050089
111465
183
183
SNP
A
5CM1
T
LH82


400
Amplicon1050089
111466
410
410
SNP
C
5CM1
T
LH82


400
Amplicon1050089
111467
468
468
SNP
A
5CM1
G
LH82


400
Amplicon1050089
111468
485
485
SNP
C
5CM1
T
LH82


400
Amplicon1050089
111469
489
489
SNP
A
LH82
G
5CM1


400
Amplicon1050089
111470
518
518
SNP
C
5CM1
T
LH82


400
Amplicon1050089
111471
527
527
SNP
A
5CM1
G
LH82


401
Amplicon1050090
111472
221
221
SNP
C
5CM1
T
LH82


402
Amplicon1050093
111475
297
297
SNP
A
LH82
G
5CM1


403
Amplicon1050119
111613
56
56
SNP
A
LH82
G
5CM1


403
Amplicon1050119
111615
113
113
SNP
C
LH82
T
5CM1


403
Amplicon1050119
111616
115
115
SNP
C
LH82
T
5CM1


403
Amplicon1050119
111617
160
160
SNP
A
LH82
T
5CM1


403
Amplicon1050119
111619
275
275
SNP
A
5CM1
G
LH82


403
Amplicon1050119
111620
551
551
IND
*
LH82
T
5CM1


404
Amplicon1050127
111628
155
155
SNP
A
LH82
G
5CM1


404
Amplicon1050127
111629
344
344
SNP
C
5CM1
T
LH82


404
Amplicon1050127
111630
345
345
SNP
A
5CM1
G
LH82


404
Amplicon1050127
111631
351
351
SNP
C
LH82
T
5CM1


404
Amplicon1050127
111632
424
424
SNP
C
5CM1
T
LH82


405
Amplicon1050172
111817
127
127
SNP
A
5CM1
G
LH82


405
Amplicon1050172
111818
255
255
SNP
A
5CM1
G
LH82


405
Amplicon1050172
111819
290
290
SNP
A
5CM1
T
LH82


405
Amplicon1050172
111820
444
444
SNP
A
5CM1
T
LH82


405
Amplicon1050172
111821
447
447
SNP
C
LH82
T
5CM1


405
Amplicon1050172
111822
448
448
SNP
A
LH82
C
5CM1


405
Amplicon1050172
111823
449
449
IND
*
5CM1
T
LH82


405
Amplicon1050172
111824
452
452
IND
*
5CM1
C
LH82


405
Amplicon1050172
111825
454
454
IND
*
5CM1
C
LH82


405
Amplicon1050172
111826
466
466
SNP
A
5CM1
G
LH82


405
Amplicon1050172
111827
511
511
SNP
C
5CM1
T
LH82


405
Amplicon1050172
111828
591
591
SNP
C
LH82
T
5CM1


406
Amplicon1050174
111829
141
141
SNP
G
5CM1
T
LH82


407
Amplicon1050237
112139
93
93
SNP
C
5CM1
G
LH82


408
Amplicon1050319
112496
121
121
SNP
A
LH82
G
5CM1


408
Amplicon1050319
112497
194
194
SNP
A
LH82
T
5CM1


409
Amplicon1050498
113113
282
282
SNP
A
LH82
G
5CM1


410
Amplicon1050539
113263
215
215
SNP
A
5CM1
G
LH82


410
Amplicon1050539
113264
238
238
SNP
C
LH82
T
5CM1


410
Amplicon1050539
113265
240
240
SNP
C
5CM1
T
LH82


410
Amplicon1050539
113266
395
395
SNP
C
LH82
G
5CM1


411
Amplicon1050570
113377
112
112
SNP
A
LH82
G
5CM1


411
Amplicon1050570
113378
137
137
SNP
C
LH82
T
5CM1


411
Amplicon1050570
113379
167
167
SNP
A
LH82
T
5CM1


411
Amplicon1050570
113380
203
203
SNP
A
5CM1
G
LH82


411
Amplicon1050570
113381
302
302
SNP
A
5CM1
G
LH82


411
Amplicon1050570
113382
412
413
IND
**
LH82
TC
5CM1


412
Amplicon1459173
143100
329
329
SNP
C
LH82
G
5CM1


412
Amplicon1459173
143101
350
350
SNP
C
5CM1
G
LH82


412
Amplicon1459173
143102
379
379
SNP
A
5CM1
C
LH82


412
Amplicon1459173
143103
413
413
IND
*
LH82
C
5CM1


412
Amplicon1459173
143104
414
415
IND
**
LH82
TC
5CM1


413
Amplicon1459183
143216
67
67
SNP
A
mo17:LH82
C
b73


413
Amplicon1459183
143217
321
321
SNP
A
b73
G
mo17:LH82


414
Amplicon1459189
143249
88
88
SNP
C
mo17:5CM1
T
b73:LH82


414
Amplicon1459189
143251
221
221
SNP
A
mo17:5CM1
G
b73:LH82


415
Amplicon1459200
143371
84
84
SNP
G
mo17
T
b73:5CM1:LH82


415
Amplicon1459200
143372
99
99
SNP
A
mo17
G
b73:5CM1:LH82


415
Amplicon1459200
143374
128
128
SNP
C
mo17
T
b73:5CM1:LH82


415
Amplicon1459200
143375
132
132
SNP
A
mo17
G
b73:5CM1:LH82


415
Amplicon1459200
143376
164
164
SNP
C
b73:mo17:LH82
G
5CM1


416
Amplicon1459201
143380
329
329
SNP
A
5CM1
G
b73:mo17:LH82


416
Amplicon1459201
143382
542
542
SNP
A
b73:mo17:LH82
C
5CM1


416
Amplicon1459201
143384
634
634
SNP
C
5CM1
T
b73:mo17:LH82


417
Amplicon1459202
143386
95
95
SNP
A
b73:mo17:LH82
C
5CM1


417
Amplicon1459202
143388
148
148
SNP
C
b73:LH82
T
mo17:5CM1


417
Amplicon1459202
143390
236
236
SNP
A
5CM1
C
b73:mo17:LH82


418
Amplicon1459206
143407
115
115
SNP
C
LH82
T
5CM1


418
Amplicon1459206
143408
381
381
SNP
G
5CM1
T
LH82


418
Amplicon1459206
143409
516
516
SNP
A
LH82
C
5CM1


419
Amplicon1459208
143413
70
70
SNP
A
5CM1
G
LH82


419
Amplicon1459208
143418
205
205
SNP
A
5CM1
T
LH82


420
Amplicon1459219
143641
113
113
SNP
A
b73:LH82
C
mo17:5CM1


420
Amplicon1459219
143642
187
187
SNP
A
LH82
T
b73:mo17:5CM1


420
Amplicon1459219
143643
209
209
SNP
A
LH82
G
b73:mo17:5CM1


420
Amplicon1459219
143644
247
247
SNP
C
mo17:5CM1:LH82
T
b73


420
Amplicon1459219
143645
285
285
SNP
A
b73:mo17:5CM1
G
LH82


420
Amplicon1459219
143646
289
289
SNP
C
LH82
T
b73:mo17:5CM1


420
Amplicon1459219
143653
707
707
IND
*
mo17
T
b73:5CM1:LH82


420
Amplicon1459219
143654
708
708
IND
*
b73:LH82
A
mo17:5CM1


420
Amplicon1459219
143657
883
883
SNP
C
mo17:5CM1
T
b73:LH82


420
Amplicon1459219
143658
946
946
SNP
A
mo17
T
b73:5CM1:LH82


420
Amplicon1459219
143659
985
985
SNP
C
mo17:5CM1:LH82
G
b73


420
Amplicon1459219
143660
1020
1020
SNP
C
b73:LH82
T
mo17:5CM1


420
Amplicon1459219
143661
1146
1146
SNP
A
b73:LH82
G
mo17:5CM1


421
Amplicon1459223
143749
65
65
SNP
C
mo17:LH82
T
b73:5CM1


421
Amplicon1459223
143750
68
68
SNP
C
mo17:LH82
T
b73:5CM1


421
Amplicon1459223
143753
104
104
SNP
A
mo17:LH82
G
b73:5CM1


421
Amplicon1459223
143754
176
176
SNP
C
mo17:LH82
T
b73:5CM1


421
Amplicon1459223
143755
195
195
SNP
C
b73:5CM1
T
mo17:LH82


422
Amplicon1459242
143969
99
100
IND
**
5CM1:LH82
TA
b73:mo17


422
Amplicon1459242
143974
520
520
IND
*
mo17
A
b73:5CM1:LH82


423
Amplicon1459247
144090
409
409
SNP
A
5CM1
G
b73:mo17:LH82


424
Amplicon1459269
144505
45
45
SNP
C
b73
T
mo17:5CM1:LH82


424
Amplicon1459269
144506
88
91
IND
****
b73
TCTA
mo17:5CM1:LH82


425
Amplicon1459275
144686
108
108
SNP
C
5CM1
G
b73:mo17:LH82


425
Amplicon1459275
144687
260
260
SNP
A
mo17:5CM1
T
b73:LH82


425
Amplicon1459275
144688
362
362
SNP
A
b73:mo17:5CM1
C
LH82


425
Amplicon1459275
144689
399
401
IND
***
b73:mo17:5CM1
ACC
LH82


426
Amplicon1459277
144731
169
169
SNP
A
b73:mo17:5CM1
G
LH82


426
Amplicon1459277
144732
238
238
SNP
A
b73:mo17:5CM1
G
LH82


427
Amplicon1459294
145065
28
28
SNP
A
b73
G
mo17:LH82


427
Amplicon1459294
145066
38
38
SNP
G
b73
T
mo17:LH82


427
Amplicon1459294
145069
110
110
SNP
C
mo17:LH82
T
b73


427
Amplicon1459294
145070
116
116
SNP
C
b73
T
mo17:LH82


427
Amplicon1459294
145071
119
119
SNP
C
mo17:LH82
T
b73


427
Amplicon1459294
145072
127
127
SNP
C
b73
T
mo17:LH82


427
Amplicon1459294
145073
133
133
SNP
A
mo17:LH82
C
b73


427
Amplicon1459294
145077
468
468
SNP
A
mo17:LH82
C
b73


428
Amplicon1459300
145200
102
102
SNP
C
b73
G
mo17:5CM1:LH82


428
Amplicon1459300
145202
176
176
SNP
A
b73
G
mo17:5CM1:LH82


428
Amplicon1459300
145203
177
177
SNP
A
b73
C
mo17:5CM1:LH82


428
Amplicon1459300
145204
271
271
SNP
C
b73
G
mo17:5CM1:LH82


428
Amplicon1459300
145205
454
457
IND
****
mo17:LH82
ACGT
b73:5CM1


429
Amplicon1459304
145260
158
158
SNP
A
5CM1
C
LH82


429
Amplicon1459304
145261
172
172
SNP
C
LH82
G
5CM1


429
Amplicon1459304
145263
235
235
SNP
C
5CM1
T
LH82


429
Amplicon1459304
145264
525
525
SNP
C
5CM1
T
LH82


429
Amplicon1459304
145266
574
574
SNP
C
5CM1
T
LH82


430
Amplicon1459307
145298
58
58
SNP
G
b73
T
mo17


430
Amplicon1459307
145299
92
92
IND
*
mo17
C
b73


430
Amplicon1459307
145300
377
377
SNP
A
b73
G
mo17


430
Amplicon1459307
145301
380
380
SNP
A
b73
G
mo17


430
Amplicon1459307
145302
393
393
SNP
G
b73
T
mo17


430
Amplicon1459307
145303
399
399
SNP
A
mo17
G
b73


430
Amplicon1459307
145304
407
407
SNP
A
b73
G
mo17


430
Amplicon1459307
145305
410
410
SNP
A
mo17
G
b73


430
Amplicon1459307
145306
416
416
SNP
A
b73
G
mo17


430
Amplicon1459307
145307
432
432
SNP
G
mo17
T
b73


430
Amplicon1459307
145308
434
434
IND
*
mo17
A
b73


430
Amplicon1459307
145309
488
488
SNP
A
mo17
C
b73


430
Amplicon1459307
145310
498
498
SNP
C
mo17
T
b73


431
Amplicon1459308
145312
244
244
SNP
A
mo17
G
b73


431
Amplicon1459308
145314
294
294
IND
*
b73
T
mo17


431
Amplicon1459308
145315
314
314
SNP
G
mo17
T
b73


431
Amplicon1459308
145316
426
426
IND
*
mo17
A
b73


431
Amplicon1459308
145317
429
429
IND
*
b73
T
mo17


431
Amplicon1459308
145318
464
464
SNP
A
mo17
G
b73


431
Amplicon1459308
145319
533
533
SNP
A
mo17
T
b73


432
Amplicon1459309
145322
912
912
SNP
C
mo17
T
b73


433
Amplicon1459322
145573
243
243
SNP
A
b73:mo17:LH82
G
5CM1


433
Amplicon1459322
145574
312
312
SNP
C
5CM1:LH82
T
b73:mo17


433
Amplicon1459322
145575
406
406
SNP
A
5CM1
G
b73:mo17:LH82


433
Amplicon1459322
145577
451
451
SNP
C
5CM1:LH82
T
b73:mo17


433
Amplicon1459322
145578
519
519
SNP
A
5CM1
T
b73:mo17:LH82


433
Amplicon1459322
145579
550
550
SNP
G
5CM1
T
b73:mo17:LH82


433
Amplicon1459322
145580
611
611
SNP
A
b73:mo17:5CM1
T
LH82


433
Amplicon1459322
145581
652
652
SNP
A
b73:mo17:LH82
C
5CM1


433
Amplicon1459322
145582
656
656
SNP
A
5CM1
G
b73:mo17:LH82


433
Amplicon1459322
145583
657
657
SNP
C
b73:mo17:LH82
T
5CM1


433
Amplicon1459322
145584
677
677
SNP
A
b73:mo17:LH82
G
5CM1


433
Amplicon1459322
145585
685
685
SNP
A
b73:mo17:LH82
C
5CM1


433
Amplicon1459322
145586
703
703
IND
*
b73:mo17
A
5CM1:LH82


433
Amplicon1459322
145587
713
734
IND
************-**********
b73:mo17:LH82
GTTGATGAAGCAGGACATTGGG
5CM1


433
Amplicon1459322
145588
784
784
SNP
A
5CM1
G
b73:mo17:LH82


433
Amplicon1459322
145590
857
857
SNP
G
b73:mo17:LH82
T
5CM1


433
Amplicon1459322
145591
864
864
SNP
A
5CM1
G
b73:mo17:LH82


433
Amplicon1459322
145592
868
868
SNP
A
b73:mo17:LH82
C
5CM1


433
Amplicon1459322
145594
877
877
IND
*
b73:mo17:LH82
C
5CM1


433
Amplicon1459322
145596
889
892
IND
****
LH82
CCAA
b73:mo17:5CM1


433
Amplicon1459322
145597
905
905
SNP
A
b73:mo17:LH82
G
5CM1


434
Amplicon1459334
145794
242
242
SNP
C
mo17:5CM1
T
b73


434
Amplicon1459334
145796
419
419
SNP
C
b73
T
mo17:5CM1


434
Amplicon1459334
145797
428
428
SNP
C
b73
T
mo17:5CM1


434
Amplicon1459334
145798
747
747
SNP
A
b73
G
mo17:5CM1


434
Amplicon1459334
145800
867
867
SNP
C
mo17:5CM1
T
b73


434
Amplicon1459334
145801
930
930
SNP
A
mo17:5CM1
T
b73


434
Amplicon1459334
145802
969
969
SNP
C
mo17:5CM1
G
b73


434
Amplicon1459334
145803
1004
1004
SNP
C
b73
T
mo17:5CM1


435
Amplicon1459354
146152
254
254
SNP
A
5CM1
G
b73:mo17:LH82


435
Amplicon1459354
146158
821
822
IND
**
5CM1
CA
b73:mo17:LH82


436
Amplicon1459358
146190
203
203
SNP
G
mo17:5CM1:LH82
T
b73


436
Amplicon1459358
146191
272
272
SNP
A
mo17:5CM1:LH82
G
b73


436
Amplicon1459358
146192
286
286
SNP
A
mo17:5CM1:LH82
C
b73


436
Amplicon1459358
146193
301
301
SNP
G
b73
T
mo17:5CM1:LH82


436
Amplicon1459358
146194
327
327
SNP
A
mo17:LH82
G
b73:5CM1


436
Amplicon1459358
146195
348
348
SNP
C
mo17:5CM1:LH82
G
b73


436
Amplicon1459358
146196
425
425
SNP
A
mo17:5CM1:LH82
G
b73


436
Amplicon1459358
146197
448
448
SNP
C
b73
G
mo17:5CM1:LH82


437
Amplicon1459369
146410
123
123
SNP
G
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146411
154
159
IND
******
5CM1
ATCTTC
b73:mo17:LH82


437
Amplicon1459369
146412
280
280
SNP
C
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146413
330
330
SNP
A
LH82
T
b73:mo17:5CM1


437
Amplicon1459369
146414
331
331
SNP
A
LH82
C
b73:mo17:5CM1


437
Amplicon1459369
146415
345
345
SNP
A
b73:LH82
G
mo17:5CM1


437
Amplicon1459369
146416
552
552
SNP
G
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146417
555
555
SNP
C
b73:mo17:LH82
G
5CM1


437
Amplicon1459369
146418
556
556
SNP
G
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146419
558
558
SNP
G
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146420
559
559
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146421
560
560
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146422
561
561
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146423
562
562
SNP
G
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146424
563
563
SNP
A
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146425
564
564
SNP
A
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146426
565
565
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146427
566
566
SNP
A
b73:mo17:LH82
C
5CM1


437
Amplicon1459369
146428
568
568
SNP
C
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146429
569
569
SNP
C
b73:mo17:LH82
G
5CM1


437
Amplicon1459369
146430
570
570
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146431
574
574
SNP
A
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146432
575
575
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146433
576
576
SNP
G
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146434
577
577
SNP
A
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146435
578
578
SNP
A
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146436
580
580
SNP
C
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146437
581
581
SNP
A
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146438
582
582
SNP
A
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146439
583
583
SNP
A
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146441
587
587
SNP
A
b73:mo17:LH82
C
5CM1


437
Amplicon1459369
146442
588
588
SNP
A
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146443
589
589
SNP
G
b73:mo17:LH82
T
5CM1


437
Amplicon1459369
146444
590
590
SNP
C
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146445
592
592
SNP
C
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146446
593
593
SNP
C
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146447
594
594
SNP
A
b73:mo17:LH82
C
5CM1


437
Amplicon1459369
146448
595
595
SNP
C
5CM1
T
b73:mo17:LH82


437
Amplicon1459369
146449
597
597
SNP
A
5CM1
G
b73:mo17:LH82


437
Amplicon1459369
146450
598
598
SNP
A
b73:mo17:LH82
C
5CM1


438
Amplicon1459371
146458
233
233
SNP
A
b73
T
mo17:5CM1:LH82


438
Amplicon1459371
146459
246
246
SNP
G
b73
T
mo17:5CM1:LH82


438
Amplicon1459371
146460
419
419
SNP
A
5CM1
G
b73:mo17:LH82


439
Amplicon1459380
146534
123
123
SNP
C
b73
T
mo17:5CM1


439
Amplicon1459380
146535
141
141
SNP
C
b73
T
mo17:5CM1


439
Amplicon1459380
146538
1015
1015
SNP
G
b73:5CM1
T
mo17


439
Amplicon1459380
146539
1017
1017
SNP
A
b73:5CM1
C
mo17


440
Amplicon1459387
146593
140
140
SNP
C
b73:mo17:5CM1
G
LH82


441
Amplicon1459392
147037
96
96
SNP
C
mo17
G
b73:5CM1:LH82


441
Amplicon1459392
147039
238
241
IND
****
b73:5CM1:LH82
CTAT
mo17


441
Amplicon1459392
147041
362
362
SNP
G
5CM1
T
b73:mo17:LH82


441
Amplicon1459392
147043
415
415
SNP
A
b73:mo17:LH82
G
5CM1


441
Amplicon1459392
147044
464
464
SNP
C
b73:mo17:LH82
T
5CM1


442
Amplicon1459402
147181
148
148
SNP
C
LH82
G
5CM1


442
Amplicon1459402
147182
183
183
SNP
A
LH82
G
5CM1


442
Amplicon1459402
147183
303
303
SNP
C
LH82
T
5CM1


442
Amplicon1459402
147184
305
305
SNP
G
5CM1
T
LH82


442
Amplicon1459402
147186
348
348
SNP
G
LH82
T
5CM1


442
Amplicon1459402
147187
396
396
SNP
C
LH82
G
5CM1


442
Amplicon1459402
147188
422
422
SNP
C
LH82
T
5CM1


442
Amplicon1459402
147189
477
485
IND
*********
5CM1
TATTGTTTT
LH82


442
Amplicon1459402
147190
488
491
IND
****
5CM1
CCTT
LH82


442
Amplicon1459402
147191
496
496
IND
*
5CM1
A
LH82


442
Amplicon1459402
147192
506
506
SNP
C
LH82
T
5CM1


443
Amplicon1459406
147216
63
63
SNP
A
b73:LH82
G
5CM1


443
Amplicon1459406
147217
64
64
SNP
C
LH82
T
b73:5CM1


443
Amplicon1459406
147218
104
104
SNP
C
LH82
T
b73:5CM1


443
Amplicon1459406
147219
145
145
SNP
A
b73:5CM1
G
LH82


443
Amplicon1459406
147220
253
253
SNP
A
b73:5CM1
G
LH82


443
Amplicon1459406
147221
324
324
SNP
A
LH82
G
b73:5CM1


443
Amplicon1459406
147222
325
325
IND
*
LH82
G
b73:5CM1


443
Amplicon1459406
147223
409
409
SNP
A
b73:5CM1
G
LH82


444
Amplicon1459414
147406
61
61
SNP
G
b73:mo17:5CM1
T
LH82


444
Amplicon1459414
147407
79
79
SNP
G
LH82
T
b73:mo17:5CM1


444
Amplicon1459414
147408
81
81
SNP
C
mo17:LH82
T
b73:5CM1


444
Amplicon1459414
147409
91
91
SNP
C
LH82
T
b73:mo17:5CM1


444
Amplicon1459414
147410
159
159
IND
*
b73:mo17:5CM1
C
LH82


444
Amplicon1459414
147411
207
207
SNP
C
mo17
T
b73:5CM1:LH82


444
Amplicon1459414
147412
415
415
SNP
C
b73
T
mo17:5CM1:LH82


444
Amplicon1459414
147414
453
453
SNP
G
b73:5CM1:LH82
T
mo17


444
Amplicon1459414
147416
493
493
SNP
C
b73:5CM1
T
mo17:LH82


445
Amplicon1459415
147417
82
82
SNP
C
b73
G
mo17:5CM1:LH82


446
Amplicon1459421
147478
165
165
SNP
C
b73:LH82
G
mo17:5CM1


446
Amplicon1459421
147480
205
205
SNP
A
b73:LH82
G
mo17:5CM1


446
Amplicon1459421
147481
226
226
SNP
A
b73:LH82
C
mo17:5CM1


446
Amplicon1459421
147482
259
259
SNP
A
b73:LH82
G
mo17:5CM1


446
Amplicon1459421
147484
316
316
SNP
A
mo17:5CM1
T
b73:LH82


446
Amplicon1459421
147485
355
355
SNP
C
b73:mo17:5CM1
T
LH82


446
Amplicon1459421
147487
375
375
SNP
A
5CM1
G
b73:mo17:LH82


446
Amplicon1459421
147488
391
391
SNP
A
mo17:5CM1
T
b73:LH82


446
Amplicon1459421
147489
400
400
SNP
A
5CM1
G
b73:mo17:LH82


446
Amplicon1459421
147496
539
539
SNP
G
b73:LH82
T
mo17:5CM1


446
Amplicon1459421
147498
577
577
SNP
A
mo17:5CM1
G
b73:LH82


446
Amplicon1459421
147499
631
631
SNP
C
b73:LH82
T
mo17:5CM1


446
Amplicon1459421
147500
650
650
SNP
A
b73:LH82
G
mo17:5CM1


446
Amplicon1459421
147501
660
660
SNP
C
b73:LH82
T
mo17:5CM1


446
Amplicon1459421
147502
740
740
SNP
A
b73:LH82
G
mo17:5CM1


446
Amplicon1459421
147504
820
820
SNP
C
b73:LH82
T
mo17:5CM1


446
Amplicon1459421
147505
822
822
SNP
C
mo17:5CM1
T
b73:LH82


446
Amplicon1459421
147506
866
866
SNP
A
mo17:5CM1
T
b73:LH82


446
Amplicon1459421
147507
907
907
IND
*
mo17
C
b73:5CM1:LH82


446
Amplicon1459421
147509
912
912
SNP
C
b73:LH82
G
mo17:5CM1


447
Amplicon1459422
147511
144
144
SNP
A
b73
G
mo17:5CM1:LH82


447
Amplicon1459422
147512
174
174
SNP
C
b73:mo17:LH82
T
5CM1


447
Amplicon1459422
147513
177
177
SNP
A
5CM1
G
b73:mo17:LH82


447
Amplicon1459422
147514
281
283
IND
***
b73:mo17:LH82
TAA
5CM1


447
Amplicon1459422
147515
334
334
SNP
A
b73:5CM1
T
mo17:LH82


447
Amplicon1459422
147516
416
416
SNP
A
5CM1
G
b73:mo17:LH82


447
Amplicon1459422
147517
508
508
SNP
C
b73
T
mo17:5CM1:LH82


447
Amplicon1459422
147518
581
581
SNP
A
mo17:LH82
C
b73:5CM1


447
Amplicon1459422
147519
621
621
SNP
A
mo17:5CM1:LH82
C
b73


447
Amplicon1459422
147520
630
630
SNP
G
mo17:5CM1:LH82
T
b73


447
Amplicon1459422
147521
641
641
SNP
G
b73:5CM1
T
mo17:LH82


447
Amplicon1459422
147522
648
648
SNP
A
mo17:LH82
T
b73:5CM1


447
Amplicon1459422
147523
656
656
SNP
G
b73:mo17:LH82
T
5CM1


447
Amplicon1459422
147525
697
697
SNP
C
b73:mo17:LH82
G
5CM1


447
Amplicon1459422
147526
734
734
SNP
C
mo17:LH82
G
b73:5CM1


447
Amplicon1459422
147527
759
759
SNP
C
5CM1
T
b73:mo17:LH82


447
Amplicon1459422
147528
788
788
SNP
C
5CM1
T
b73:mo17:LH82


447
Amplicon1459422
147529
791
791
SNP
C
b73
G
mo17:5CM1:LH82


447
Amplicon1459422
147530
792
792
SNP
A
b73
G
mo17:5CM1:LH82


447
Amplicon1459422
147531
830
830
SNP
C
b73:mo17:LH82
T
5CM1


447
Amplicon1459422
147532
849
849
SNP
C
b73:mo17:LH82
T
5CM1


447
Amplicon1459422
147533
868
868
SNP
C
mo17:LH82
T
b73:5CM1


447
Amplicon1459422
147535
908
908
SNP
A
5CM1
C
b73:mo17:LH82


447
Amplicon1459422
147536
921
921
SNP
A
5CM1
G
b73:mo17:LH82


447
Amplicon1459422
147537
928
928
SNP
G
b73:mo17:LH82
T
5CM1


447
Amplicon1459422
147538
943
947
IND
*****
5CM1
CTTGT
b73:mo17:LH82


447
Amplicon1459422
147539
961
961
SNP
C
mo17:5CM1:LH82
T
b73


447
Amplicon1459422
147540
966
966
SNP
A
b73:mo17:LH82
T
5CM1


447
Amplicon1459422
147541
1142
1142
SNP
G
b73:mo17:LH82
T
5CM1


448
Amplicon1459423
147542
183
183
SNP
C
b73:5CM1:LH82
T
mo17


448
Amplicon1459423
147543
455
455
SNP
C
mo17
T
b73:5CM1:LH82


448
Amplicon1459423
147544
533
540
IND
********
b73:5CM1:LH82
ATTGAAAT
mo17


448
Amplicon1459423
147545
650
650
SNP
C
mo17
T
b73:5CM1:LH82


448
Amplicon1459423
147546
851
851
SNP
C
mo17
T
b73:5CM1:LH82


448
Amplicon1459423
147548
1046
1046
SNP
A
mo17
G
b73:5CM1:LH82


449
Amplicon1459443
147767
279
279
SNP
G
b73:mo17:LH82
T
5CM1


449
Amplicon1459443
147768
429
429
SNP
C
mo17:5CM1:LH82
T
b73


449
Amplicon1459443
147771
483
483
SNP
C
b73:mo17:LH82
T
5CM1


449
Amplicon1459443
147772
620
620
SNP
A
b73
T
mo17:5CM1:LH82


449
Amplicon1459443
147773
627
627
SNP
A
mo17:5CM1:LH82
G
b73


450
Amplicon1460617
148018
61
61
SNP
C
mo17
G
b73:LH82


450
Amplicon1460617
148019
112
112
SNP
A
b73:LH82
C
mo17


450
Amplicon1460617
148020
224
224
SNP
G
b73:LH82
T
mo17


450
Amplicon1460617
148021
228
228
SNP
C
b73
G
mo17:LH82


450
Amplicon1460617
148022
235
235
SNP
A
LH82
C
b73:mo17


450
Amplicon1460617
148023
245
247
IND
***
mo17:LH82
GTC
b73


450
Amplicon1460617
148024
283
291
IND
*********
mo17
ACGTACGGT
b73:LH82


450
Amplicon1460617
148026
329
332
IND
****
mo17:LH82
GTAC
b73


450
Amplicon1460617
148027
354
357
IND
****
b73:mo17
CATC
LH82


450
Amplicon1460617
148028
382
383
IND
**
mo17:LH82
CA
b73


450
Amplicon1460617
148029
426
431
IND
******
b73:LH82
CTGTGT
mo17


450
Amplicon1460617
148030
442
442
SNP
C
b73:LH82
G
mo17


450
Amplicon1460617
148031
454
456
IND
***
b73:LH82
TAC
mo17


450
Amplicon1460617
148032
462
462
SNP
C
mo17
G
b73:LH82


451
Amplicon1460632
148155
229
229
SNP
A
b73:LH82
C
mo17:5CM1


451
Amplicon1460632
148156
280
280
SNP
A
mo17:5CM1
C
b73:LH82


451
Amplicon1460632
148157
546
546
SNP
A
b73:LH82
C
mo17:5CM1


451
Amplicon1460632
148158
587
587
SNP
A
mo17:5CM1:LH82
G
b73


451
Amplicon1460632
148159
613
613
SNP
C
mo17:5CM1:LH82
T
b73


452
Amplicon1460638
148194
467
467
SNP
A
5CM1
G
b73


453
Amplicon1460644
148039
94
94
SNP
C
b73:5CM1
T
mo17:LH82


453
Amplicon1460644
148040
115
115
SNP
A
LH82
C
b73:mo17:5CM1


453
Amplicon1460644
148041
125
125
SNP
C
5CM1
T
b73:mo17:LH82


453
Amplicon1460644
148042
139
139
IND
*
5CM1
C
b73:mo17:LH82


453
Amplicon1460644
148043
146
146
SNP
A
mo17
C
b73:5CM1:LH82


453
Amplicon1460644
148044
171
171
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148045
190
190
SNP
A
b73:mo17:5CM1
C
LH82


453
Amplicon1460644
148046
192
192
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148047
209
209
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148048
217
217
SNP
C
b73:mo17:5CM1
T
LH82


453
Amplicon1460644
148049
222
222
SNP
A
LH82
G
b73:mo17:5CM1


453
Amplicon1460644
148050
252
252
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148051
258
258
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148052
273
273
SNP
A
b73:5CM1:LH82
G
mo17


453
Amplicon1460644
148053
290
290
SNP
A
b73
G
mo17:5CM1:LH82


453
Amplicon1460644
148054
295
295
SNP
A
mo17:LH82
G
b73:5CM


453
Amplicon1460644
148055
308
308
SNP
C
mo17
T
b73:5CM1:LH82


453
Amplicon1460644
148056
325
339
IND
***************
b73:5CM1
CCTTCGATGATAATG
mo17
TCTTCGATGATAATG
LH82


453
Amplicon1460644
148057
342
356
IND
***************
mo17
TTCGACGATGACGCC
b73
TTCGATGATGACGCC
5CM1
TTCGATGATG-ATGCC
LH82


453
Amplicon1460644
148058
359
359
SNP
C
mo17:LH82
G
b73:5CM1


453
Amplicon1460644
148059
360
360
SNP
C
mo17:LH82
T
b73:5CM1


453
Amplicon1460644
148060
367
367
SNP
C
b73:5CM1
T
mo17:LH82


453
Amplicon1460644
148061
372
372
SNP
A
mo17:LH82
G
b73:5CM1


453
Amplicon1460644
148062
375
375
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148063
378
378
SNP
G
b73:5CM1:LH82
T
mo17


453
Amplicon1460644
148064
382
382
SNP
A
LH82
C
b73:mo17:5CM1


453
Amplicon1460644
148065
384
384
SNP
A
b73:5CM1
G
mo17:LH82


453
Amplicon1460644
148066
393
393
SNP
A
b73:5CM1:LH82
G
mo17


453
Amplicon1460644
148067
399
399
SNP
A
mo17
G
b73:5CM1:LH82


453
Amplicon1460644
148068
424
424
SNP
C
mo17:LH82
T
b73:5CM1


453
Amplicon1460644
148069
432
432
SNP
A
b73
G
mo17:5CM1:LH82


454
Amplicon1460702
148621
441
441
SNP
A
LH82
G
b73:mo17:5CM1


455
Amplicon1460724
148362
274
274
SNP
A
5CM1
G
b73:LH82


455
Amplicon1460724
148364
425
425
SNP
A
b73:LH82
C
5CM1


455
Amplicon1460724
148367
548
548
SNP
G
5CM1
T
b73:LH82


455
Amplicon1460724
148380
600
600
SNP
C
b73:LH82
G
5CM1


455
Amplicon1460724
148381
601
601
SNP
A
b73:LH82
T
5CM1


455
Amplicon1460724
148382
605
605
SNP
A
5CM1
T
b73:LH82


455
Amplicon1460724
148384
618
618
SNP
G
b73:LH82
T
5CM1


456
Amplicon1461863
151469
54
54
SNP
C
b73:mo17:5CM1
T
LH82


456
Amplicon1461863
151470
63
63
SNP
C
b73:mo17:LH82
T
5CM1


456
Amplicon1461863
151471
69
69
SNP
C
b73:mo17
G
5CM1:LH82


456
Amplicon1461863
151472
93
93
SNP
A
LH82
G
b73:mo17:5CM1


456
Amplicon1461863
151473
114
114
SNP
C
LH82
T
b73:mo17:5CM1


456
Amplicon1461863
151474
144
144
SNP
A
5CM1
G
b73:mo17:LH82


456
Amplicon1461863
151475
223
228
IND
******
LH82
TTCGGT
b73:mo17:5CM1


456
Amplicon1461863
151476
240
240
SNP
C
b73:mo17:LH82
T
5CM1


456
Amplicon1461863
151477
249
249
SNP
A
b73:mo17:LH82
C
5CM1


456
Amplicon1461863
151478
253
253
SNP
G
5CM1
T
b73:mo17:LH82


456
Amplicon1461863
151479
258
258
SNP
A
b73:mo17
G
5CM1:LH82


456
Amplicon1461863
151480
268
270
IND
***
b73:mo17:LH82
GAG
5CM1


456
Amplicon1461863
151481
297
297
SNP
C
b73:mo17:LH82
T
5CM1


456
Amplicon1461863
151482
318
318
SNP
C
b73:mo17:LH82
T
5CM1


456
Amplicon1461863
151483
408
408
SNP
A
b73:mo17:LH82
G
5CM1


456
Amplicon1461863
151484
450
450
SNP
A
5CM1
G
b73:mo17:LH82


456
Amplicon1461863
151485
459
459
SNP
A
b73:mo17:LH82
G
5CM1


456
Amplicon1461863
151486
524
524
SNP
C
b73:mo17
G
5CM1:LH82


457
Amplicon1461872
151382
224
224
SNP
A
b73:LH82
C
5CM1


457
Amplicon1461872
151384
418
418
SNP
C
5CM1
G
b73:LH82


457
Amplicon1461872
151385
444
444
SNP
C
b73:LH82
G
5CM1


457
Amplicon1461872
151386
531
531
SNP
A
b73:LH82
T
5CM1


457
Amplicon1461872
151388
534
534
IND
*
b73
G
5CM1:LH82


457
Amplicon1461872
151389
534
536
IND
***
b73:LH82
GCG
5CM1


457
Amplicon1461872
151390
539
543
IND
*****
b73:LH82
TTGCC
5CM1


457
Amplicon1461872
151391
558
559
IND
**
LH82
*A
b73
GA
5CM1


457
Amplicon1461872
151392
562
562
IND
*
b73
A
5CM1:LH82


457
Amplicon1461872
151393
568
568
SNP
C
5CM1
G
b73:LH82


457
Amplicon1461872
151396
638
640
IND
***
5CM1
GGC
b73:LH82


457
Amplicon1461872
151397
642
642
IND
*
5CM1
T
b73:LH82


458
Amplicon1461878
151271
267
267
SNP
C
b73
T
5CM1


458
Amplicon1461878
151273
398
398
SNP
G
5CM1
T
b73


458
Amplicon1461878
151274
406
406
SNP
C
b73
T
5CM1


458
Amplicon1461878
151276
465
465
SNP
C
b73
T
5CM1


458
Amplicon1461878
151277
468
468
SNP
A
b73
C
5CM1


458
Amplicon1461878
151280
1079
1079
SNP
A
5CM1
G
b73


458
Amplicon1461878
151281
1142
1144
IND
***
5CM1
GAG
b73


458
Amplicon1461878
151282
1155
1155
SNP
A
b73
G
5CM1


458
Amplicon1461878
151283
1167
1167
SNP
A
5CM1
G
b73


458
Amplicon1461878
151284
1180
1180
SNP
A
b73
G
5CM1


458
Amplicon1461878
151285
1280
1280
SNP
A
b73
T
5CM1


458
Amplicon1461878
151287
1323
1323
SNP
C
5CM1
T
b73


458
Amplicon1461878
151288
1419
1419
SNP
A
b73
G
5CM1


458
Amplicon1461878
151289
1447
1447
SNP
C
5CM1
T
b73


458
Amplicon1461878
151290
1586
1586
SNP
A
5CM1
G
b73


459
Amplicon1461887
151356
119
119
SNP
A
LH82
G
b73:mo17:5CM1


459
Amplicon1461887
151357
200
200
SNP
A
b73:mo17:5CM1
C
LH82


459
Amplicon1461887
151358
201
201
SNP
C
b73:mo17:5CM1
T
LH82


459
Amplicon1461887
151359
420
420
SNP
A
b73:mo17:5CM1
G
LH82


459
Amplicon1461887
151360
621
621
SNP
A
b73
G
mo17:5CM1:LH82


459
Amplicon1461887
151362
642
642
SNP
C
b73:mo17:5CM1
T
LH82


459
Amplicon1461887
151363
644
644
SNP
A
b73:mo17:5CM1
T
LH82


459
Amplicon1461887
151364
645
645
SNP
A
LH82
C
b73:mo17:5CM1


460
Amplicon1462250
153431
205
205
SNP
A
b73:mo17:LH82
G
5CM1


460
Amplicon1462250
153432
325
325
SNP
A
b73:mo17:LH82
G
5CM1


460
Amplicon1462250
153433
391
391
SNP
A
5CM1
G
b73:mo17:LH82


460
Amplicon1462250
153434
529
529
SNP
A
5CM1
G
b73:mo17:LH82


461
Amplicon1462274
153427
79
79
SNP
C
mo17:5CM1
T
b73:LH82


462
Amplicon1462278
153630
55
55
SNP
A
b73:5CM1
T
LH82


462
Amplicon1462278
153631
59
59
SNP
C
b73:5CM1
T
LH82


462
Amplicon1462278
153632
103
103
SNP
G
5CM1:LH82
T
b73


462
Amplicon1462278
153633
141
141
IND
*
b73:LH82
A
5CM1


462
Amplicon1462278
153636
210
210
SNP
C
b73:5CM1
T
LH82


462
Amplicon1462278
153637
245
245
SNP
G
b73:LH82
T
5CM1


462
Amplicon1462278
153638
399
399
SNP
C
5CM1
T
b73:LH82


462
Amplicon1462278
153639
408
415
IND
********
5CM1
GCCAAGTC
b73:LH82


462
Amplicon1462278
153640
418
420
IND
***
5CM1
CAG
b73:LH82


462
Amplicon1462278
153641
423
427
IND
*****
5CM1
AGCCA
b73:LH82


462
Amplicon1462278
153642
433
433
SNP
A
5CM1
C
b73:LH82


462
Amplicon1462278
153643
434
434
SNP
C
5CM1
G
b73:LH82


462
Amplicon1462278
153644
435
435
SNP
A
5CM1
T
b73:LH82


462
Amplicon1462278
153645
454
457
IND
****
5CM1
ATCA
b73:LH82


462
Amplicon1462278
153646
467
467
SNP
A
b73:LH82
T
5CM1


462
Amplicon1462278
153647
468
468
SNP
C
b73:LH82
G
5CM1


462
Amplicon1462278
153648
470
470
SNP
C
5CM1
G
b73:LH82


462
Amplicon1462278
153649
503
503
SNP
A
b73:LH82
C
5CM1


462
Amplicon1462278
153650
527
527
SNP
A
b73:LH82
C
5CM1


462
Amplicon1462278
153651
548
548
IND
*
5CM1
T
b73:LH82


462
Amplicon1462278
153652
672
672
SNP
A
b73:LH82
G
5CM1


463
Amplicon1462297
153856
137
138
IND
**
b73:LH82
AC
5CM1


463
Amplicon1462297
153857
151
151
SNP
A
5CM1
T
b73:LH82


463
Amplicon1462297
153858
160
163
IND
****
LH82
AGCT
b73:5CM1


463
Amplicon1462297
153860
197
197
SNP
C
5CM1:LH82
T
b73


463
Amplicon1462297
153861
201
201
SNP
A
5CM1:LH82
G
b73


463
Amplicon1462297
153862
207
207
SNP
G
b73:5CM1
T
LH82


463
Amplicon1462297
153863
221
221
SNP
C
b73:LH82
T
5CM1


463
Amplicon1462297
153864
232
232
SNP
A
5CM1
C
b73:LH82


463
Amplicon1462297
153865
270
276
IND
*******
b73:5CM1
TCTCCCG
LH82


463
Amplicon1462297
153866
292
292
SNP
G
5CM1
T
b73:LH82


463
Amplicon1462297
153867
302
303
IND
**
b73
GG
5CM1:LH82


463
Amplicon1462297
153868
303
322
IND
************-********
5CM1
***CAGAGCATGGTACCGAG
b73
GGGCAGAGCATGGTACCGAG
LH82


463
Amplicon1462297
153869
324
324
SNP
C
LH82
T
b73:5CM1


463
Amplicon1462297
153870
334
338
IND
*****
LH82
TATGA
b73:5CM1


463
Amplicon1462297
153871
374
374
SNP
A
LH82
G
b73:5CM1


463
Amplicon1462297
153872
389
389
SNP
C
b73
T
5CM1:LH82


463
Amplicon1462297
153873
392
392
SNP
C
5CM1:LH82
T
b73


463
Amplicon1462297
153874
411
411
IND
*
b73:LH82
T
5CM1


463
Amplicon1462297
153875
414
414
IND
*
5CM1
C
b73:LH82


463
Amplicon1462297
153876
420
420
SNP
A
b73:5CM1
G
LH82


463
Amplicon1462297
153877
443
447
IND
*****
5CM1:LH82
TTAAA
b73


463
Amplicon1462297
153878
472
472
SNP
A
5CM1:LH82
G
b73


463
Amplicon1462297
153879
553
553
SNP
C
b73
G
5CM1:LH82


463
Amplicon1462297
153880
714
714
SNP
C
b73
T
5CM1:LH82


463
Amplicon1462297
153881
715
715
SNP
A
5CM1:LH82
T
b73


464
Amplicon1462302
153412
260
260
SNP
C
5CM1:LH82
T
b73


464
Amplicon1462302
153413
286
286
SNP
A
5CM1:LH82
G
b73


464
Amplicon1462302
153414
320
320
SNP
A
b73
G
5CM1:LH82


464
Amplicon1462302
153415
430
430
SNP
C
b73
T
5CM1:LH82


464
Amplicon1462302
153416
434
434
SNP
C
b73
T
5CM1:LH82


464
Amplicon1462302
153417
492
492
SNP
A
5CM1:LH82
G
b73


464
Amplicon1462302
153420
1001
1001
SNP
A
5CM1:LH82
T
b73


464
Amplicon1462302
153423
1032
1032
SNP
G
5CM1:LH82
T
b73


464
Amplicon1462302
153424
1042
1042
SNP
A
b73
T
5CM1:LH82


464
Amplicon1462302
153425
1493
1493
SNP
A
b73
T
5CM1:LH82


464
Amplicon1462302
153426
1495
1495
SNP
A
b73
G
5CM1:LH82


465
Amplicon1462306
153885
53
53
SNP
C
5CM1:LH82
T
b73


465
Amplicon1462306
153886
86
86
SNP
A
b73
G
5CM1:LH82


465
Amplicon1462306
153887
311
311
IND
*
b73
C
5CM1:LH82


465
Amplicon1462306
153889
352
352
SNP
A
5CM1:LH82
C
b73


465
Amplicon1462306
153890
372
372
IND
*
b73
T
5CM1:LH82


465
Amplicon1462306
153891
479
479
SNP
A
b73
G
5CM1:LH82


465
Amplicon1462306
153892
577
577
SNP
C
5CM1:LH82
T
b73


465
Amplicon1462306
153893
601
601
SNP
G
5CM1:LH82
T
b73


465
Amplicon1462306
153894
1032
1032
SNP
A
5CM1:LH82
T
b73


465
Amplicon1462306
153895
1090
1090
SNP
G
5CM1:LH82
T
b73


465
Amplicon1462306
153897
1166
1166
SNP
C
b73
G
5CM1:LH82


465
Amplicon1462306
153898
1214
1214
SNP
C
b73
T
5CM1:LH82


465
Amplicon1462306
153901
1276
1276
SNP
C
b73
T
5CM1:LH82


465
Amplicon1462306
153902
1297
1297
SNP
G
b73
T
5CM1:LH82


465
Amplicon1462306
153903
1362
1362
SNP
C
b73
T
5CM1:LH82


465
Amplicon1462306
153904
1374
1374
SNP
A
5CM1:LH82
G
b73


465
Amplicon1462306
153905
1380
1383
IND
****
b73
ATAT
5CM1:LH82


465
Amplicon1462306
153906
1385
1385
SNP
C
b73
T
5CM1:LH82


465
Amplicon1462306
153907
1412
1412
SNP
A
5CM1:LH82
T
b73


465
Amplicon1462306
153908
1414
1414
SNP
A
5CM1:LH82
G
b73


465
Amplicon1462306
153909
1466
1466
SNP
C
5CM1:LH82
T
b73


465
Amplicon1462306
153910
1469
1469
SNP
C
b73
T
5CM1:LH82


465
Amplicon1462306
153911
1516
1516
SNP
C
b73
G
5CM1:LH82


465
Amplicon1462306
153912
1555
1555
SNP
C
b73
T
5CM1:LH82


465
Amplicon1462306
153913
1562
1562
SNP
C
5CM1:LH82
G
b73


465
Amplicon1462306
153914
1567
1567
SNP
C
5CM1:LH82
T
b73


465
Amplicon1462306
153915
1580
1581
IND
**
5CM1:LH82
AC
b73


465
Amplicon1462306
153916
1585
1590
IND
******
5CM1:LH82
TTATTA
b73


465
Amplicon1462306
153917
1597
1597
SNP
A
b73
C
5CM1:LH82


465
Amplicon1462306
153918
1608
1608
SNP
C
5CM1:LH82
T
b73


466
Amplicon1463332
153971
33
33
SNP
C
b73:LH82
T
5CM1


466
Amplicon1463332
153972
108
108
SNP
G
5CM1
T
b73:LH82


466
Amplicon1463332
153973
110
110
SNP
A
b73:LH82
C
5CM1


466
Amplicon1463332
153974
116
116
IND
*
b73:LH82
T
5CM1


466
Amplicon1463332
153975
141
141
SNP
A
b73:LH82
G
5CM1


466
Amplicon1463332
153976
148
148
IND
*
b73:LH82
A
5CM1


466
Amplicon1463332
153977
170
170
SNP
C
5CM1
G
b73:LH82


466
Amplicon1463332
153978
180
180
SNP
A
5CM1
G
b73:LH82


466
Amplicon1463332
153979
181
181
SNP
C
b73:LH82
T
5CM1


466
Amplicon1463332
153980
214
214
SNP
C
5CM1
T
b73:LH82


466
Amplicon1463332
153981
313
313
SNP
A
b73:LH82
G
5CM1


466
Amplicon1463332
153982
331
331
SNP
C
b73:LH82
G
5CM1


466
Amplicon1463332
153985
463
463
SNP
A
5CM1
C
b73:LH82


466
Amplicon1463332
153987
485
485
SNP
C
5CM1
T
b73:LH82


466
Amplicon1463332
153993
971
971
SNP
C
b73:LH82
T
5CM1


466
Amplicon1463332
153994
1124
1124
SNP
C
b73:LH82
T
5CM1


466
Amplicon1463332
153995
1146
1146
SNP
A
5CM1
G
b73:LH82


466
Amplicon1463332
153996
1178
1207
IND
************-************-******
b73:LH82
GGTCCATGGTGCACTTAGAAAGAACAGTCT
5CM1


466
Amplicon1463332
153997
1215
1215
SNP
A
b73:LH82
G
5CM1


466
Amplicon1463332
153998
1289
1289
SNP
A
b73:LH82
G
5CM1


466
Amplicon1463332
153999
1424
1424
SNP
C
5CM1
T
b73:LH82


467
Amplicon1463341
154038
65
65
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154039
76
76
SNP
G
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154040
78
78
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154041
98
98
SNP
G
b73:5CM1
T
mo17:LH82


467
Amplicon1463341
154042
163
163
SNP
A
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154043
168
168
SNP
C
mo17:LH82
T
b73:5CM1


467
Amplicon1463341
154044
177
177
SNP
A
mo17:LH82
G
b73:5CM1


467
Amplicon1463341
154045
179
179
SNP
A
b73:5CM1
G
mo17:LH82


467
Amplicon1463341
154046
184
184
SNP
A
mo17:LH82
G
b73:5CM1


467
Amplicon1463341
154047
187
187
SNP
G
b73:5CM1
T
mo17:LH82


467
Amplicon1463341
154048
192
192
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154049
236
250
IND
***************
mo17:LH82
CCAGAGGCACGCGCC
b73:5CM1


467
Amplicon1463341
154052
317
318
IND
**
5CM1
CT
b73:mo17:LH82


467
Amplicon1463341
154053
395
395
SNP
A
mo17:LH82
G
b73:5CM1


467
Amplicon1463341
154054
493
493
SNP
A
b73:5CM1
C
mo17:LH82


467
Amplicon1463341
154055
503
503
SNP
A
b73
C
mo17:5CM1:LH82


467
Amplicon1463341
154056
504
504
SNP
C
5CM1
G
b73:mo17:LH82


467
Amplicon1463341
154057
538
538
SNP
C
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154058
557
557
SNP
G
mo17:LH82
T
b73:5CM1


467
Amplicon1463341
154059
602
602
SNP
C
b73:mo17:LH82
T
5CM1


467
Amplicon1463341
154060
606
606
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154061
679
679
SNP
G
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154062
681
681
SNP
C
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154063
682
682
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154064
683
683
SNP
C
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154065
684
684
SNP
A
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154066
687
687
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154067
688
688
SNP
A
b73
C
mo17:5CM1:LH82


467
Amplicon1463341
154068
690
690
SNP
C
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154069
691
691
SNP
G
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154070
692
692
SNP
A
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154071
693
693
SNP
C
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154072
694
694
SNP
G
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154073
695
695
SNP
A
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154074
696
696
SNP
A
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154075
697
697
SNP
A
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154076
698
698
SNP
G
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154077
699
699
SNP
A
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154078
700
700
SNP
G
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154079
702
702
SNP
A
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154080
704
704
SNP
G
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154081
705
705
SNP
A
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154082
706
706
SNP
A
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154083
708
708
SNP
G
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154084
709
709
SNP
A
b73
C
mo17:5CM1:LH82


467
Amplicon1463341
154085
711
711
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154086
712
712
SNP
A
mo17:5CM1:LH82
C
b73


467
Amplicon1463341
154087
713
713
SNP
A
b73
C
mo17:5CM1:LH82


467
Amplicon1463341
154088
714
714
SNP
A
mo17:5CM1:LH82
G
b73


467
Amplicon1463341
154089
715
715
SNP
A
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154090
717
717
SNP
A
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154091
718
718
SNP
A
b73:mo17:LH82
G
5CM1


467
Amplicon1463341
154092
719
719
SNP
A
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154093
720
720
SNP
C
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154094
721
721
SNP
A
b73
G
mo17:5CM1:LH82


467
Amplicon1463341
154095
722
722
SNP
C
mo17:5CM1:LH82
T
b73


467
Amplicon1463341
154096
723
723
SNP
A
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154097
724
724
SNP
G
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154098
726
726
SNP
C
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154099
727
727
SNP
A
b73
T
mo17:5CM1:LH82


467
Amplicon1463341
154100
728
728
SNP
A
mo17:5CM1:LH82
C
b73


467
Amplicon1463341
154101
729
729
SNP
C
mo17:5CM1:LH82
T
b73


468
Amplicon1463358
154007
50
50
SNP
A
5CM1
G
b73:LH82


468
Amplicon1463358
154008
73
73
SNP
A
b73:LH82
G
5CM1


468
Amplicon1463358
154009
208
208
SNP
C
5CM1
T
b73:LH82


468
Amplicon1463358
154010
282
282
SNP
C
5CM1
T
b73:LH82


468
Amplicon1463358
154011
292
321
IND
************-************-******
b73:LH82
ACTGTTCTTTCTAAGTGCACCATGGACCAG
5CM1


468
Amplicon1463358
154012
351
351
SNP
C
b73:LH82
T
5CM1


468
Amplicon1463358
154013
373
373
SNP
A
5CM1
G
b73:LH82


468
Amplicon1463358
154014
526
526
SNP
A
5CM1
G
b73:LH82


468
Amplicon1463358
154020
927
927
SNP
C
b73:LH82
T
5CM1


468
Amplicon1463358
154021
1012
1012
SNP
A
b73:LH82
G
5CM1


468
Amplicon1463358
154023
1034
1034
SNP
G
b73:LH82
T
5CM1


468
Amplicon1463358
154026
1166
1166
SNP
C
5CM1
G
b73:LH82


468
Amplicon1463358
154027
1184
1184
SNP
C
5CM1
T
b73:LH82


468
Amplicon1463358
154028
1283
1283
SNP
A
b73:LH82
G
5CM1


468
Amplicon1463358
154029
1316
1316
SNP
A
5CM1
G
b73:LH82


468
Amplicon1463358
154030
1317
1317
SNP
C
b73:LH82
T
5CM1


468
Amplicon1463358
154031
1327
1327
SNP
C
b73:LH82
G
5CM1


468
Amplicon1463358
154032
1350
1350
IND
*
b73:LH82
T
5CM1


468
Amplicon1463358
154033
1356
1356
SNP
C
5CM1
T
b73:LH82


468
Amplicon1463358
154034
1383
1383
IND
*
b73:LH82
A
5CM1


468
Amplicon1463358
154035
1387
1387
SNP
G
5CM1
T
b73:LH82


468
Amplicon1463358
154036
1389
1389
SNP
A
b73:LH82
C
5CM1


468
Amplicon1463358
154037
1464
1464
SNP
A
5CM1
G
b73:LH82


469
Amplicon1463815
154534
64
64
SNP
A
5CM1
G
b73:mo17:LH82


469
Amplicon1463815
154535
100
100
SNP
C
b73:mo17:LH82
T
5CM1


469
Amplicon1463815
154536
238
238
SNP
C
b73:mo17:LH82
T
5CM1


469
Amplicon1463815
154537
304
304
SNP
C
5CM1
T
b73:mo17:LH82


470
Amplicon1463828
155152
30
31
IND
**
b73:5CM1
TA
LH82


470
Amplicon1463828
155153
83
93
IND
***********
LH82
ATATATATACA
b73:5CM1


470
Amplicon1463828
155154
158
158
SNP
A
b73:5CM1
T
LH82


470
Amplicon1463828
155155
289
289
SNP
C
LH82
G
b73:5CM1


470
Amplicon1463828
155156
290
290
SNP
A
b73:5CM1
T
LH82


470
Amplicon1463828
155157
338
338
SNP
C
LH82
T
b73:5CM1


470
Amplicon1463828
155158
346
346
SNP
C
LH82
G
b73:5CM1


470
Amplicon1463828
155159
457
457
SNP
A
b73:5CM1
G
LH82


471
Amplicon1463835
154552
194
194
SNP
A
b73:LH82
C
5CM1


471
Amplicon1463835
154553
271
271
SNP
C
5CM1
T
b73:LH82


471
Amplicon1463835
154554
276
276
SNP
A
b73:LH82
T
5CM1


471
Amplicon1463835
154555
330
330
SNP
A
b73:LH82
G
5CM1


471
Amplicon1463835
154557
388
388
SNP
A
5CM1
G
b73:LH82


471
Amplicon1463835
154558
395
395
SNP
A
5CM1
G
b73:LH82


471
Amplicon1463835
154559
433
436
IND
****
b73:LH82
AATT
5CM1


471
Amplicon1463835
154560
627
627
IND
*
5CM1
G
b73:LH82


471
Amplicon1463835
154561
633
633
SNP
C
5CM1
T
b73:LH82


471
Amplicon1463835
154562
680
680
SNP
C
b73:LH82
T
5CM1


471
Amplicon1463835
154563
764
764
SNP
A
b73:LH82
G
5CM1


471
Amplicon1463835
154564
859
859
SNP
A
5CM1
T
b73:LH82


471
Amplicon1463835
154565
874
874
SNP
C
b73:LH82
T
5CM1


471
Amplicon1463835
154567
1063
1063
SNP
A
5CM1
G
b73:LH82


471
Amplicon1463835
154568
1099
1099
SNP
C
b73:LH82
T
5CM1


471
Amplicon1463835
154569
1237
1237
SNP
C
b73:LH82
T
5CM1


472
Amplicon1463848
154530
79
79
SNP
A
5CM1
G
b73:mo17:LH82


472
Amplicon1463848
154531
115
115
SNP
C
b73:mo17:LH82
T
5CM1


472
Amplicon1463848
154532
253
253
SNP
C
b73:mo17:LH82
T
5CM1


472
Amplicon1463848
154533
319
319
SNP
C
5CM1
T
b73:mo17:LH82


473
Amplicon1463850
154616
221
221
SNP
A
b73
C
5CM1


473
Amplicon1463850
154617
298
298
SNP
C
5CM1
T
b73


473
Amplicon1463850
154618
303
303
SNP
A
b73
T
5CM1


473
Amplicon1463850
154619
357
357
SNP
A
b73
G
5CM1


473
Amplicon1463850
154621
415
415
SNP
A
5CM1
G
b73


473
Amplicon1463850
154622
422
422
SNP
A
5CM1
G
b73


473
Amplicon1463850
154623
460
461
IND
**
b73
AA
5CM1


473
Amplicon1463850
154624
463
463
IND
*
b73
T
5CM1


473
Amplicon1463850
154626
609
609
SNP
C
5CM1
T
b73


473
Amplicon1463850
154627
707
707
SNP
C
b73
T
5CM1


473
Amplicon1463850
154628
791
791
SNP
A
b73
G
5CM1


473
Amplicon1463850
154629
886
886
SNP
A
5CM1
T
b73


473
Amplicon1463850
154630
901
901
SNP
C
b73
T
5CM1


473
Amplicon1463850
154632
1090
1090
SNP
A
5CM1
G
b73


473
Amplicon1463850
154633
1126
1126
SNP
C
b73
T
5CM1


474
Amplicon1463853
154505
131
131
SNP
C
b73:mo17:LH82
T
5CM1


474
Amplicon1463853
154506
335
335
SNP
C
5CM1
T
b73:mo17:LH82


475
Amplicon1463858
154511
221
221
SNP
A
b73:LH82
C
5CM1


475
Amplicon1463858
154512
298
298
SNP
C
5CM1
T
b73:LH82


475
Amplicon1463858
154513
303
303
SNP
A
b73:LH82
T
5CM1


475
Amplicon1463858
154514
357
357
SNP
A
b73:LH82
G
5CM1


475
Amplicon1463858
154516
415
415
SNP
A
5CM1
G
b73:LH82


475
Amplicon1463858
154517
422
422
SNP
A
5CM1
G
b73:LH82


475
Amplicon1463858
154518
460
464
IND
****T
LH82
**T**
b73
AATTT
5CM1


475
Amplicon1463858
154519
609
609
SNP
C
5CM1
T
b73:LH82


475
Amplicon1463858
154520
650
650
IND
*
5CM1
A
b73:LH82


475
Amplicon1463858
154521
654
654
IND
*
5CM1
G
b73:LH82


475
Amplicon1463858
154522
660
660
SNP
C
5CM1
T
b73:LH82


475
Amplicon1463858
154523
707
707
SNP
C
b73:LH82
T
5CM1


475
Amplicon1463858
154524
791
791
SNP
A
b73:LH82
G
5CM1


475
Amplicon1463858
154526
901
901
SNP
C
b73:LH82
T
5CM1


475
Amplicon1463858
154528
1090
1090
SNP
A
5CM1
G
b73:LH82


475
Amplicon1463858
154529
1126
1126
SNP
C
b73:LH82
T
5CM1


476
Amplicon1463864
154843
93
95
IND
***
b73:mo17
CAC
5CM1


476
Amplicon1463864
154844
114
114
IND
*
b73:5CM1
T
mo17


476
Amplicon1463864
154845
169
169
SNP
A
5CM1
G
b73:mo17


476
Amplicon1463864
154846
251
257
IND
*******
mo17
CGGGGCG
b73:5CM1


476
Amplicon1463864
154849
289
289
IND
*
mo17:5CM1
G
b73


476
Amplicon1463864
154853
459
461
IND
***
b73:5CM1
AAG
mo17


476
Amplicon1463864
154854
467
467
SNP
G
mo17:5CM1
T
b73


476
Amplicon1463864
154855
471
471
SNP
A
5CM1
G
b73:mo17


476
Amplicon1463864
154856
479
479
SNP
C
b73:5CM1
T
mo17


476
Amplicon1463864
154857
498
499
IND
**
mo17
GC
b73:5CM1


476
Amplicon1463864
154858
501
501
IND
*
mo17
G
b73:5CM1


477
Amplicon1463878
154507
62
62
SNP
A
5CM1
G
b73:mo17:LH82


477
Amplicon1463878
154508
98
98
SNP
C
b73:mo17:LH82
T
5CM1


477
Amplicon1463878
154509
236
236
SNP
C
b73:mo17:LH82
T
5CM1


477
Amplicon1463878
154510
302
302
SNP
C
5CM1
T
b73:mo17:LH82


478
Amplicon1464068
155475
92
92
SNP
A
b73
T
LH82


479
Amplicon1464087
155688
97
97
SNP
C
b73:mo17:LH82
T
5CM1


479
Amplicon1464087
155689
235
235
SNP
C
b73:mo17:LH82
T
5CM1


479
Amplicon1464087
155690
301
301
SNP
C
5CM1
T
b73:LH82


480
Amplicon1464088
155691
273
273
SNP
A
5CM1
G
b73:mo17:LH82


480
Amplicon1464088
155692
309
309
SNP
C
b73:mo17:LH82
T
5CM1


480
Amplicon1464088
155696
608
608
SNP
C
5CM1
T
b73:LH82


480
Amplicon1464088
155697
692
692
SNP
A
5CM1
G
b73:LH82


480
Amplicon1464088
155702
977
977
SNP
C
b73:LH82
T
5CM1


480
Amplicon1464088
155703
984
984
SNP
C
b73:LH82
T
5CM1


480
Amplicon1464088
155705
1042
1042
SNP
C
5CM1
T
b73:LH82


480
Amplicon1464088
155706
1096
1096
SNP
A
5CM1
T
b73:LH82


480
Amplicon1464088
155707
1101
1101
SNP
A
b73:LH82
G
5CM1


480
Amplicon1464088
155708
1178
1178
SNP
G
5CM1
T
b73:LH82


481
Amplicon1464097
155827
341
341
SNP
A
LH82
T
b73


481
Amplicon1464097
155828
347
347
SNP
C
b73
G
LH82


481
Amplicon1464097
155829
417
417
SNP
A
LH82
G
b73


481
Amplicon1464097
155830
484
484
SNP
C
b73
T
LH82


481
Amplicon1464097
155831
510
510
IND
*
b73
T
LH82


481
Amplicon1464097
155832
511
511
IND
*
b73
A
LH82


481
Amplicon1464097
155833
620
620
SNP
C
LH82
T
b73


481
Amplicon1464097
155835
811
811
SNP
C
b73
T
LH82


481
Amplicon1464097
155836
814
814
SNP
C
LH82
T
b73


481
Amplicon1464097
155837
865
865
SNP
A
b73
G
LH82


481
Amplicon1464097
155838
872
872
SNP
A
b73
G
LH82


481
Amplicon1464097
155839
881
881
SNP
C
b73
T
LH82


481
Amplicon1464097
155840
892
892
SNP
A
b73
C
LH82


481
Amplicon1464097
155841
950
950
SNP
A
LH82
T
b73


481
Amplicon1464097
155842
978
978
SNP
G
b73
T
LH82


481
Amplicon1464097
155843
987
987
SNP
A
b73
G
LH82


481
Amplicon1464097
155844
1035
1035
SNP
A
LH82
G
b73


481
Amplicon1464097
155845
1100
1100
SNP
A
b73
C
LH82


481
Amplicon1464097
155846
1133
1133
SNP
C
b73
T
LH82


481
Amplicon1464097
155848
1192
1192
SNP
G
b73
T
LH82


481
Amplicon1464097
155849
1215
1215
SNP
G
b73
T
LH82


481
Amplicon1464097
155850
1239
1239
SNP
A
LH82
G
b73


481
Amplicon1464097
155851
1264
1264
SNP
G
LH82
T
b73


481
Amplicon1464097
155852
1265
1265
SNP
C
b73
G
LH82


481
Amplicon1464097
155853
1273
1273
SNP
A
LH82
T
b73


482
Amplicon1464098
155861
117
117
SNP
C
b73:5CM1
T
mo17:LH82


482
Amplicon1464098
155862
138
138
SNP
A
LH82
C
b73:mo17:5CM1


482
Amplicon1464098
155863
148
148
SNP
C
5CM1
T
b73:mo17:LH82


482
Amplicon1464098
155864
162
162
IND
*
5CM1
C
b73:mo17:LH82


482
Amplicon1464098
155865
169
169
SNP
A
mo17
C
b73:5CM1:LH82


482
Amplicon1464098
155866
194
194
SNP
A
b73:5CM1
G
mo17:LH82


482
Amplicon1464098
155867
213
213
SNP
A
b73:mo17:5CM1
C
LH82


482
Amplicon1464098
155868
215
215
SNP
A
b73:5CM1
G
mo17:LH82


482
Amplicon1464098
155869
232
232
SNP
A
b73:5CM1
G
mo17:LH82


482
Amplicon1464098
155870
240
240
SNP
C
b73:mo17:5CM1
T
LH82


482
Amplicon1464098
155871
245
245
SNP
A
LH82
G
b73:mo17:5CM1


482
Amplicon1464098
155872
275
275
SNP
A
b73:5CM1
G
mo17:LH82


482
Amplicon1464098
155873
281
281
SNP
A
b73:5CM1
G
mo17:LH82


482
Amplicon1464098
155874
296
296
SNP
A
b73:5CM1:LH82
G
mo17


482
Amplicon1464098
155875
313
313
SNP
A
b73
G
mo17:5CM1:LH82


482
Amplicon1464098
155876
318
318
SNP
A
mo17:LH82
G
b73:5CM1


482
Amplicon1464098
155877
331
331
SNP
C
mo17
T
b73:5CM1:LH82


482
Amplicon1464098
155878
348
362
IND
***************
b73:5CM1
TCTTCGATGATAATG
LH82


482
Amplicon1464098
155879
370
370
SNP
C
b73
T
5CM1:LH82


482
Amplicon1464098
155880
376
387
IND
************
LH82
CGCCGAGTATGA
b73:5CM1


482
Amplicon1464098
155881
401
406
IND
******
b73:5CM1
GTCACC
mo17:LH82


482
Amplicon1464098
155882
414
419
IND
ATGACG
LH82
ATGCCG
mo17
C***CA
b73:5CM1


482
Amplicon1464098
155883
428
428
SNP
A
b73:5CM1:LH82
G
mo17


482
Amplicon1464098
155884
434
434
SNP
A
mo17
G
b73:5CM1:LH82


482
Amplicon1464098
155885
534
536
IND
***
LH82
CAA
b73:mo17:5CM1


482
Amplicon1464098
155886
551
551
SNP
C
b73:mo17
G
5CM1:LH82


483
Amplicon1464112
155790
40
40
SNP
G
b73:LH82
T
5CM1


483
Amplicon1464112
155791
72
72
SNP
A
5CM1
G
b73:LH82


483
Amplicon1464112
155792
78
78
SNP
A
5CM1
G
b73:LH82


483
Amplicon1464112
155793
132
132
SNP
C
b73:LH82
T
5CM1


483
Amplicon1464112
155794
151
151
SNP
A
b73:LH82
G
5CM1


483
Amplicon1464112
155795
161
161
SNP
C
b73:LH82
T
5CM1


483
Amplicon1464112
155796
241
241
SNP
A
b73:LH82
G
5CM1


483
Amplicon1464112
155797
252
252
SNP
C
5CM1
T
b73:LH82


483
Amplicon1464112
155798
321
321
SNP
C
b73:LH82
T
5CM1


483
Amplicon1464112
155799
323
323
SNP
C
5CM1
T
b73:LH82


483
Amplicon1464112
155800
367
367
SNP
A
5CM1
T
b73:LH82


483
Amplicon1464112
155801
409
409
IND
*
5CM1
C
b73:LH82


483
Amplicon1464112
155802
413
413
SNP
C
b73:LH82
G
5CM1


484
Amplicon1464142
156240
92
94
IND
***
b73
ATA
5CM1:LH82


484
Amplicon1464142
156241
133
133
SNP
A
LH82
C
5CM1
G
b73


484
Amplicon1464142
156242
214
214
SNP
A
b73
T
mo17:5CM1:LH82


484
Amplicon1464142
156243
247
247
SNP
A
b73
C
mo17:5CM1:LH82


484
Amplicon1464142
156245
286
286
SNP
C
mo17:LH82
G
5CM1
T
b73


484
Amplicon1464142
156247
370
370
SNP
A
mo17:LH82
G
b73:5CM1


484
Amplicon1464142
156248
411
411
SNP
A
b73:LH82
C
5CM1
T
mo17


484
Amplicon1464142
156249
548
548
SNP
A
b73
C
mo17:LH82


484
Amplicon1464142
156251
582
582
SNP
C
mo17:LH82
T
b73


484
Amplicon1464142
156252
593
593
SNP
A
mo17:LH82
G
b73

## Claims

1. A method of identifying oil-associated QTLs in maize comprising
(a) establishing a population of maize plants derived from the Illinois high oil line and/or the Illinois low oil line,
(b) selecting a set of oil informative allelic polymorphic markers from at least 40 loci from the set of polymorphic markers listed in Table 3,
(c) using said markers to identify oil-associated QTLs in said maize plants.