MLB Summary of Disinfectant Products Tested via the OECD Quantitative MethodAHydrogenperoxideLiquid 0.5 H * RTU NT 1 min. Yes N/A 5 6.6E+06 2 2.6E+06 3.0≥5.9 5 min. N/A 0.500 Y≥6.7≥6.2 4.9E+06 1.7E+06B Phenol Liquid 19 H * 1 + 255 NT 10 min. Yes400 ppmhard water2 5.3E+06 3 1.4E+07 6.0 5.5 5 min.375 ppmhard water0.074 Y≤3.7 3.3 5.0E+06 1.3E+06C Phenol Liquid 22.5 H * 1 + 255 NT 10 min. Yes500 ppmhard water35 1.4E+07 16 7.9E+06≥6.8≥5.7 5 min.375 ppmhard water0.088 Y 4.7 3.0 4.8E+06 9.5E+05D Phenol Liquid 16.75 H * 1 + 127 NT 10 min. Yes400 ppmhard water0 1.6E+06 1 1.9E+06≥6.7≥5.9 5 min.375 ppmhard water0.131 Y 6.5≥6.2 4.9E+06 1.7E+06E Quat Liquid 3.85 H * 3 + 125 NT 10 min. Yes400 ppmhard water0 1.2E+07 5 8.6E+06 4.6 0.6 5 min.375 ppmhard water0.090 Y≤3.7≤1.1 5.0E+06 1.3E+06F Quat Liquid 16.9 H * 1 + 255 NT 10 min. Yes400 ppmhard water0 3.8E+06 7 9.1E+06 4.1≤0.9 5 min.375 ppmhard water0.066 Y 2.8≤1.1 5.8E+06 1.3E+06G Quat Liquid 2.113 H * 1 + 31 NT 10 min. Yes400 ppmhard water0 6.5E+06 48 1.1E+07 4.1≤0.9 5 min.375 ppmhard water0.066 Y 2.3≤1.1 5.8E+06 1.3E+06H Quat Liquid 3.85 H * 1 + 63 NT 10 min. Yes250 ppmhard water0 4.6E+06 4 7.6E+06 6.3≤0.8 5 min.375 ppmhard water0.060 Y 2.0≤1.0 5.2E+06 9.1E+05I Quat Liquid 1.1856 S * NT 1 + 15 1 min. No DI water N/A N/A N/A N/A≤1.7≤0.9 5 min.375 ppmhard water0.074 Y≤1.7≤1.2 5.0E+06 1.6E+06J Quat + HCl Liquid 0.05 + 9 H * 1 + 2 NT 10 min. Yes Tap water 0 5.1E+06 0 4.1E+06≥6.9≥5.8 5 min.375 ppmhard water0.017 + 3 Y≥6.7≥6.0 5.2E+06 9.1E+05KSodiumhypochloriteLiquid 6 H * 1 + 13.8 NT 5 min. Yes DI water 2 9.3E+06 2 2.4E+06≥6.8≥5.7 5 min.375 ppmhard water0.405 Y≥6.7≥6.0 4.8E+06 9.5E+05LSodiumhypochloriteLiquid 6.15 H * 5.33 + 128 NT 5 min. No DI water 0 8.3E+06 0 2.9E+06≥6.7≥5.9 5 min.375 ppmhard water0.246 Y≥6.7≥6.2 5.0E+06 1.6E+06M Citric acid Liquid 0.6 H * RTU NT 5 min. Yes N/A 1 9.8E+06 0 6.1E+06≥6.7≥5.9 5 min. N/A 0.600 Y≥6.5≥6.4 3.4E+06 2.3E+06The OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides (OECD method) recently underwent a multi-laboratory ring trial and the EPA is interested in adopting the OECD method for regulatory purposes. As part of the implementationprocess, a series of studies was conducted to evaluate the logreduction values of currently registered liquid antimicrobial products using the OECD procedure. Over the course of this study, 13 different products were evaluated against bothStaphylococcus aureusandPseudomonas aeruginosausing parameters specified for the UDM (label claim) and also with standard OECD parametersAll use-dilutions were based on product label claims. For the purposes of comparing products with 2 sets of claims, a side-by-side comparative evaluation was conducted (the order of the parameters tested within a test day was randomized). Products wereevaluated first againstS. aureusand then againstP. aeruginosa.OECD TEST METHOD PARAMETERS/RESULTSContacttime (min)DiluentCarrierCounts(S.a.)Efficacy(P.a.)PositivecarriersCarrierCounts(P.a.)3 PartOSLused?(Y or N)ControlCarrierCount -P.a.ControlCarrierCount -S.a.UseDilutionOECDParametersLog ReductionS.a.**OECDParametersLog ReductionP.a.**Label ClaimLogReductionS.a.**Label ClaimLogReductionP.a.**ATP RESULTSEfficacy(S.a.)PositivecarriersOECD TEST METHODRESULTS -- LABELCLAIM PARAMETERSATP PARAMETERSPRODUCT EFFICACY USE DIRECTIONSProductIDProductFormatProductEfficacyClaim (astested)ProductUseDirectionsChemicalClassification ofProductActive(s)PercentActive(s)declared onthe labelUse-dilutionforSanitizationUse-DilutionforDisinfectionDiluentContactTime(min)OSL(Y/N)*Parameters are developed using the product label, original studies, and the master label.**Values listed as≥represent complete kill. Values listed as≤represent a value of 1000 that was substituted at the least concentrated dilution because growth on the filter was a lawn.Product ID - corresponds to product identifical list as provided by EPAChemical Classifivation of Product Active(s) - corresponds to the product active as declared on the label. This could be one active (Quaternary, Citric Acid, Phenolic)Product Format - this corresponds to the format the product is being sold in. This could be a concentrated liquid dilutable, atrigger spray, pump spray or aerosol spray product for examplePercent Active(s) declared on the label - this is the percentage of the Active(s) as declared on the product labelProduct Efficacy Claim (as tested) - this corresponds to the product efficacy category that was tested. This includes HospitalGrade Disinfectants (H) and Sanitizers (S)Product Use Directions - this corresponds to where the use directions were derived from including the product graphic Label (L)or Master Label (ML)Use-dilution for Disinfection - this corresponds to the use-directions on how to apply the product for use as a disinfectant.For example Ready to Use (RTU) or product dilution instructions. NT = not testedUse-dilution for Sanitization - this corresponds to the use-directions on how to apply the product for use as a sanitizer. Forexample Ready to Use (RTU) or product dilution instructions. NT = not testedContact Time (min) - this corresponds to the contact time the product needs for that efficacy category.OSL (Y/N) - this corresponds to the use of an organic soil load in the Use-Dilution testDiluent - this corresponds to the diluent used in the Use-Dilution testATP Results - Efficacy (S.a.) - this corresponds to the efficacy data determined through ATP testing. Value given is the number of positive carriers out of 60.ATP Results - Carrier Count (S.a.) - this corresponds to the average dried carrier count data. For example 1.0 x 10^6 CFU/mLATP Results - Efficacy (P.a.) - this corresponds to the efficay data determination through ATP testing. Value given is the number of positive carriers out of 60.ATP Results - Carrier Counts (P.a.) - this corresponds to the average dried carrier count data. For example 1.0 x 10^6 CFU/mLOECD Test Method Results -- Label Claim Parameters - Label Claim Log Reduction S.a. - this corresponds to the log reduction when tested at the label claim for this organismOECD Test Method Results -- Label Claim Parameters - Label Claim Log Reduction P.a. - this corresponds to the log reduction when tested at the label claim for this organismContact Time (min) - this corresponds to the contact time product where product efficacy testing was performedDiluent - this corresponds to the diluent used for product dilution for efficacy testingUse-dilution - this corresponds to the final product concentration during testing3 Part OSL (organic soil load) Used - this corresponds to whether or not the 3 part organic soil in the method was used in thetestingOECD Parameters Log Reduction S.a. - this corresponds to the log reduction when tested with the OECD parameters for this organismOECD Parameters Log Reduction P.a. - this corresponds to the log reduction when tested with the OECD parameters for this organismControl Carrier Count (S.a.) - this corresponds to the dried carrier control count for this organismControl Carrier Count (P.a.) - this corresponds to the the dried carrier control count for this organism

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*

† * *

* * *

† †

† * * *

*

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0

A B C D E F G H I J K L MLog10Reduction 

# S . aureus Log Reduction using Label Claim

and OECD Test Parameters

Label Claim

OECD

Hydrogen

peroxide Phenol Sodium

hypochlorite Quaternary ammonium

compounds Quat +

HCl Citric

acid



# Test Chemicals

* = Value listed represents complete kill.

† = Value listed represents a value of 1,000 that was substituted at the least concentrated dilution because growth on the fi lte r was a lawn.

# *

* *

† † † † * * * * * *

† † † † † * * * *

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0

A B C D E F G H I J K L MLog10Reduction 

# P. aeruginosa Log Reduction using Label Claim

and OECD Test Parameters

Label Claim

OECD

Hydrogen

peroxide Phenol Sodium

hypochlorite Quaternary ammonium

compounds Quat +

HCl Citric

acid



# Test Chemicals

* = Value listed represents complete kill.

† = Value listed represents a value of 1,000 that was substituted at the least concentrated dilution because growth on the fi lte r was a lawn.