Effluent Quality Assessment 

Florida Power and Light Company 

St. Lucie Plant 

St. Lucie County 

NPDES #FL0002208 

Sampled April 5, 2010 

June 2010

Biology Section 

Bureau of Laboratories 

Division of Environmental Assessment and Restoration 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1 

CONTENTS

Introduction 

Methods 

Results and Discussion 

Literature Cited 

Tables 

Table 1 Measured algal growth potential (AGP) 

Table 2 Effluent limits, Class III Marine Criteria and chemical data 

Appendices 

Appendix A Chain of Custody 

Appendix B Bioassay Bench Sheets 

Appendix C SRT Bench Sheets and Statistical Analyses 

Appendix D Organism Receipt 

Appendix E Chemical Analyses of Effluent 

Appendix F Facility Summary, Effluent Limits, and Permit Violations 

Recorded on Discharge Monitoring Report 

Appendix G EPA End Sheet 
2 

Florida Power & Light Company – St. Lucie Plant, 6501 S. Ocean Drive, Jensen Beach, St. Lucie 

County, Florida, 34957. NPDES #FL0002208. Effluent grab samples for bioassay and chemical 

analyses for this facility were collected on April 5, 2010. 

Introduction

Florida Power & Light Company – St. Lucie Plant is a nuclear power plant which treats industrial 

wastewater through neutralization, settling, ion exchange, and microfiltration. (See Appendix F for 

detailed facility information provided by Terry Davis of FDEP Southeast District.) 

Methods 

Samples were collected from the EFF-2 NPDES sampling point for Outfall D-001 by Terry Davis 

and Danielle Melnick (FDEP Southeast District) according to permit requirements. Two five-gallon 

portions of the once-through cooling water (OTCW) were obtained from the discharge from Unit 

#2 and 8 mL of a chemical solution consisting of 30 ppm ETA, 25 ppm N2H4, and 20 ppm CH2

was added to each to simulate normal discharge. The samples for all analyses were taken from 

the two five-gallon portions. 

See Appendix A for the completed chain of custody form. 

The 96-hour acute screening toxicity tests and algal growth potential test were performed 

following internal DEP SOPs TA07_03, TA07_04, and TA08_01 through TA08_09. All internal 

DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. 

The salinity of the sample was adjusted from an initial salinity of 36.4 ppt to a final salinity of 34.9 

ppt by the addition of deionized water, in order to conform to the method requirements for 

saltwater test species. 

Results and Discussion 

Toxicity Test Results 

Tests were performed April 6 -10, 2010. 

Americamysis bahia 96-hour acute screening bioassay – LC50 > 100%, 5%* mortality in 

 100% sample at 96 hours 

Menidia beryllina 96-hour acute screening bioassay – LC50 > 100%, 0%* mortality in 

 100% sample at 96 hours 

The bioassay samples were not acutely toxic to the test organisms. 

*NOTE: In a 96-hour acute screening test of 100% effluent, mortality of <20% provides 

confidence that the effluent meets the acute toxicity criterion of Florida’s Surface Water 

Quality Standards (Rule 62-302.500 (1) (a) 4, F.A.C.). Mortality between 20-50% indicates 

low to moderate levels of toxicity, and further action may be required. Mortality of >50% 

indicates that the effluent fails to meet the minimum requirement to discharge to waters of the 

state (Rule 62-4.244(3) (a), F.A.C.). 

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 

standard reference toxicant (SRT) tests and the bench sheets and statistical analyses of the 

SRT tests that correspond to these facility tests. See Appendix D for test organism receipts. 
3 

Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was 3.0 U (U - Material was analyzed for but not 

detected; the value reported is the minimum detection limit) mg dry wt/L of the saltwater 

species Dunaliella tertiolecta. Raschke and Schultz found that AGP values above 10.0 mg 

dry weight/L represent a “problem” threshold for marine receiving waters, implying nutrient 

enrichment (personal communication, Marshall Faircloth, et. al. FDEP, with Ron Raschke 

USEPA Region 4 1987). The ratio (17.0:1) of total nitrogen to total phosphorus suggests that 

the effluent is co-limited by both nitrogen and phosphorus. The ratio (2.9:1) of inorganic 

nitrogen and orthophosphate suggest that the effluent is nitrogen limited (Maloney et al. 

1978). 

See Table 1 for AGP results. 

Chemistry Results 

Total residual chlorine was not detected, and total ammonia was not measured in the 

bioassay sample in the laboratory. The total ammonia concentration in the composite sample 

collected and preserved for chemical analysis was 0.022 mg N/L. Based on the pH, salinity, 

and temperature of the effluent as collected, the calculated unionized ammonia concentration 

was < 0.02 mg/L. 

Aluminum was detected in the effluent at levels that comply with Class III marine water 

quality criteria (62-302.530, F.A.C.). Arsenic was detected between the laboratory method 

detection limits (MDL) and practical quantitation limits (PQL). Oils and Greases were 

detected in the equipment blank, but not in the effluent. 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See 

Appendix E for a complete list of chemical analyses performed. 

Conclusion 

The effluent sample collected from the EFF-2 NPDES sampling point for Outfall D-001 for this 

facility on April 5, 2010, was not acutely toxic to either test species during the 96-hour acute 

screening bioassays. The effluent AGP result was less than the "problem” threshold for marine 

receiving waters. Effluent water quality samples collected on April 5, 2010, did not exceed any 

applicable water quality standards or violate any permit conditions. 

Literature Cited 

Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: Bottle 

Test. National Environmental Research Center Office of Research and Development. U. S. 

EPA, Corvallis, Oregon. 43 p. 
4 

Inorganic Total

Location N:P ratio N:P ratio

D-001 3.0 U 2.9 17.0

U - Material analyzed for but not detected; value reported is the method detection limit

Table 1. Measured algal growth potential (AGP; mg dry 

weight/L) of the saltwater species Dunaliella tertiolecta 

and ratios of nitrogen to phosphorus for samples 

collected from the FPL - St. Lucie Plant Outfall on April 5, 

2010.

AGP

FPL Co - St. Lucie Plant 

NPDES# FL0002208

(measured)
5 

FPL Co - St. Lucie Plant 

NPDES# FL0002208

Class III 

Criteria

Effluent 

Limits

Aluminum ≤ 1500 - 1400

Arsenic ≤ 50 - 1.6 I

Cadmium ≤ 8.8 - 0.09 U

Calcium (mg/L) - - 421

Chromium-III - - 0.9 U

Copper ≤ 3.7 - 1.5 U

Iron ≤ 300 - 90 U

Lead ≤ 8.5 - 0.6 U

Magnesium (mg/L) - - 1380

Nickel ≤ 8.3 - 0.75 U

Selenium ≤ 71 - 0.6 U

Silver ≤ 2.3 - 0.075 U

Zinc ≤ 86 - 15 U

Ortho-phosphate - - 0.013 I

Total Phosphorus - - 0.021

Total Ammonia - - 0.022

Un-ionized Ammonia - - ≤ 0.02 c

Nitrate and Nitrite - - 0.016

Total Kjeldahl Nitrogen - - 0.34 I

Organic Nitrogen - - 0.318 c

Total Nitrogen - - 0.356 c

Dissolved Oxygen (mg/L) ≥ 4.0 - 6.6 £

pH (SU) 6.5 - 8.5 - 7.7

Conductivity (μmhos/cm) - - 55580

Temperature (°C) - ≤ 45 34.3

Salinity - - 30.4 c

Total Residual Chlorine (mg/L) ≤ 0.01 - ≤ 0.03 UQ£

Chlorophyll a (μg/L) - Corrected - - 0.55 U

Phaeophytin (μg/L) - - 0.4 U

Total Suspended Solids (mg/L) - - 13 I

Oils and Greases (mg/L) ≤ 5.0 - 1.4 UF

Flow (MGD) - Report 1,487 a

Hardness (mg CaCO3) - - 6734.08 c

Value exceeds the Class III Water Quality Criteria or permit limits

a - Annual average

c - Value is calculated

F - Analyte was detected in the equipment blank (1.5 I mg/L)

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit

U - Material analyzed for but not detected; value reported is the method detection limit

Q - Sample held beyond normal holding time

£ - Measured in Central Laboratory Toxicity lab

General Physical and Chemical Parameters

Table 2. Effluent limits, Class III Criteria for predominantly marine waters and 

chemical data for samples collected from the Florida Power & Light Company - St. 

Lucie Plant on April 5, 2010.

D-001

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)
6 

Appendix A. Chain of Custody 

Form
7 

Appendix B. Bench Sheets 
8 

Appendix B. (continued) 
9 

Appendix B. (continued) 
10 

Appendix B. (continued) 
11 

Appendix B. (continued) 
12 

Appendix B. (continued) 
13 

Appendix C. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 96-hour Acute Toxicity

Americamysis bahia (mysid shrimp) - Cadmium Chloride (CdCl2) 

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

10/07/08 21.89 27.66 4.73 18.21 37.11

11/12/08 25.50 27.40 4.69 18.01 36.78

12/02/08 27.31 27.13 4.53 18.07 36.20

01/06/09 31.20 26.79 3.86 19.07 34.50

02/03/09 25.44 26.56 3.79 18.97 34.15

03/03/09 24.49 26.78 3.52 19.75 33.81

04/08/09 24.49 26.45 3.39 19.66 33.23

05/05/09 27.98 26.39 3.36 19.68 33.10

06/09/09 22.89 26.09 3.39 19.32 32.87

07/07/09 30.00 26.43 3.43 19.57 33.28

08/04/09 26.14 26.65 3.24 20.16 33.13

09/01/09 22.89 26.69 3.19 20.30 33.07

09/29/09 22.46 26.17 2.97 20.22 32.11

11/03/09 23.12 26.08 3.04 20.00 32.15

12/1/09 31.91 26.03 2.94 20.16 31.91

1/5/10 25.00 25.91 2.92 20.06 31.75

2/2/10 26.18 25.77 2.84 20.09 31.46

2/9/10 41.45 26.68 4.46 17.76 35.59

3/3/10 28.31 26.78 4.47 17.84 35.72

4/6/10 24.49 26.66 4.50 17.66 35.65

CV = 16.88

Lower Confidence Limit = mean - 2 * Std Dev EPA reference 75th quartile CV = 26%

Upper Confidence Limit = mean + 2 * Std Dev

10

15

20

25

30

35

40

45

50

10/07/0811/12/0812/02/0801/06/0902/03/0903/03/0904/08/0905/05/0906/09/0907/07/0908/04/0909/01/0909/29/0911/03/09

12/1/09

1/5/102/2/102/9/103/3/104/6/10

μg/L CdCl2

Standard Reference Toxicant Data 

Americamysis bahia 96 hour LC50

LC50 Mean LC50 Lower Control Limit Upper Control Limit
14 

Appendix C. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 96-hour Acute Toxicity

Menidia beryllina (inland silverside) - Copper Chloride (CuCl2) as Cu

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

11/04/08 249.81 305.57 62.50 180.57 430.57

12/02/08 316.79 308.13 61.82 184.48 431.78

01/06/09 253.83 308.45 61.51 185.44 431.46

02/03/09 245.51 304.15 62.80 178.55 429.74

03/03/09 416.64 307.01 66.63 173.74 440.27

03/31/09 228.94 300.89 67.96 164.97 436.80

05/05/09 326.07 300.24 67.64 164.97 435.52

06/02/09 465.39 302.54 72.50 157.54 447.53

06/09/09 355.67 302.52 72.48 157.55 447.48

07/07/09 403.35 307.19 75.91 155.37 459.02

08/04/09 343.29 306.49 75.48 155.52 457.45

09/09/09 402.57 311.84 78.40 155.03 468.65

09/29/09 497.5 318.42 88.10 142.21 494.62

10/13/09 426.5 326.12 90.57 144.97 502.27

11/3/09 441.06 329.60 93.69 142.22 516.99

12/1/09 390.28 335.59 93.54 148.51 522.68

1/5/10 387.66 343.92 90.17 163.57 524.26

2/9/10 306.1 350.85 80.73 189.39 512.30

3/3/10 298.9 353.79 77.48 198.82 508.76

3/30/10 333.83 354.48 77.23 200.03 508.94

CV = 21.79

EPA reference 75th quartile CV = 21%

0

100

200

300

400

500

600

11/04/0812/02/0801/06/0902/03/0903/03/0903/31/0905/05/0906/02/0906/09/0907/07/0908/04/0909/09/0909/29/0910/13/09

11/3/0912/1/09

1/5/102/9/103/3/10

3/30/10

μg/L Cu

Test Date

Standard Reference Toxicant Data 

Menidia beryllina 96-hour LC50

LC50 Mean LC50 Lower Control Limit Upper Control Limit
15 

Appendix C. (continued) 
16 

Appendix C. (continued) 
17 

Appendix C. (continued) 
18 

Appendix C. (continued) 
19 

Appendix C. (continued) 

Americamysis bahia Acute Standard Reference Toxicant Test

DATE: 4‐6‐10       DURATION: 96 hr       TOXICANT: CdCl2

SPECIES:  Americamysis bahia

RAW DATA:  Concentration       Number      Mortalities

   (μg/L)             Exposed

                    .00                    20             0

                 20.00                    20             5

                 30.00                    20            15

                 45.00                    20            18

                 67.00                    20            20

                           100.00                   20            20

SPEARMAN‐KARBER TRIM: 25.00%

SPEARMAN‐KARBER ESTIMATES: LC50: 24.49

                95% LOWER CONFIDENCE:        21.92

                95% UPPER CONFIDENCE:         27.37

Menidia beryllina Acute Standa rd Re ference Toxicant Test

DATE: 3‐30‐10       DURATION: 96 hr       TOXICANT: CuCl2

SPECIES:  Menidia beryllina

RAW DATA:  Concentration       Number      Mortalities

   (μg/L)              Exposed

                        .00                    20             1

                 100.00                    20             0

                 200.00                    20             1

                 300.00                    20             4

                 400.00                    20            17

                 500.00                    20            20

  SPEARMAN‐KARBER TRIM: 0%

  SPEARMAN‐KARBER ESTIMATES: LC50: 333.83

                 95% LOWER CONFIDENCE:         307.51

                 95% UPPER CONFIDENCE:          362.40

NOTE:  MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING.  ADJUSTMENTS WERE

MADE PRIOR TO SPEARMAN‐KARBER ESTIMATION.
20 

Appendix D. Test Organism Receipts 
21 

Appendix D. (continued) 
22 

Appendix E. Chemical Analyses performed on the effluent from the Florida Power & Light – St. 

D-001, sampled on April 5, 2010.

Date Sampled Field ID Analysis Group Components Result Remark Units MD

4/5/2010 12:20 D-001 AGP/LN Algal Growth Potential 3 U mg DryWt/L 3 4/5/2010 12:20 D-001 Chlorophyll/Grain Size/BOD Phaeophytin-a 0.4 U ug/L 0.4 4/5/2010 12:20 D-001 Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 0.55 U ug/L 0.54/5/2010 12:20 D-001 Toxicology BSY-AS-SW-Mysid, LC50 100 L LC50 4/5/2010 12:20 D-001 Toxicology BSY-AS-SW-Fish, LC50 100 L LC50 4/5/2010 12:20 D-001 Metals Selenium 0.6 U ug/L 0.6 4/5/2010 12:20 D-001 Metals Nickel 0.75 U ug/L 0.74/5/2010 12:20 D-001 Metals Magnesium 1380 mg/L 2 4/5/2010 12:20 D-001 Metals Cadmium 0.09 U ug/L 0.04/5/2010 12:20 D-001 Metals Aluminum 1400 ug/L 184/5/2010 12:20 D-001 Metals Iron 90 U ug/L 90 4/5/2010 12:20 D-001 Metals Chromium 0.9 U ug/L 0.9 4/5/2010 12:20 D-001 Metals Zinc 15 U ug/L 15 4/5/2010 12:20 D-001 Metals Calcium 421 mg/L 0.24/5/2010 12:20 D-001 Metals Silver 0.075 U ug/L 0.04/5/2010 12:20 D-001 Metals Arsenic 1.6 I ug/L 0.74/5/2010 12:20 D-001 Metals Copper 1.5 U ug/L 1.5 4/5/2010 12:20 D-001 Metals Lead 0.6 U ug/L 0.6 4/5/2010 12:20 D-001 Nutrients Kjeldahl Nitrogen 0.34 I mg N/L 0.14/5/2010 12:20 D-001 Nutrients Total-P 0.021 mg P/L 0.04/5/2010 12:20 D-001 Nutrients Ammonia-N 0.022 mg N/L 0.04/5/2010 12:20 D-001 Nutrients NO2NO3-N 0.016 mg N/L 0.04/5/2010 12:20 D-001 Nutrients O-Phosphate-P 0.013 I mg P/L 0.04/5/2010 12:20 D-001 Nutrients TSS 13 I mg/L 4 4/5/2010 12:20 D-001 Overflow Oil and Grease 1.4 U mg/L 1.4 4/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(a)anthracene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Diethyl phthalate 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(k)fluoranthene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Dibenzo(a,h)anthracene 0.93 U ug/L 0.9

4/5/2010 12:20 D-001 Priority Organic Pollutants Hexachlorobenzene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Hexachloroethane 2.8 U ug/L 2.8 
23 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remark Units MD

4/5/2010 12:20 D-001 Priority Organic Pollutants Naphthalene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 2-Chlorophenol 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 14 4/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(b)fluoranthene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Phenanthrene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 2,4,6-Trichlorophenol 0.93 U ug/L 0.9

4/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 14 4/5/2010 12:20 D-001 Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.8 U ug/L 2.8 4/5/2010 12:20 D-001 Priority Organic Pollutants 4-Chloro-3-methylphenol 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 14 UJ ug/L 14 4/5/2010 12:20 D-001 Priority Organic Pollutants Dimethyl phthalate 9.3 U ug/L 9.3 4/5/2010 12:20 D-001 Priority Organic Pollutants Pentachlorophenol 2.8 U ug/L 2.8 4/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dinitrotoluene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 3,3'-Dichlorobenzidine 37 U ug/L 37 4/5/2010 12:20 D-001 Priority Organic Pollutants Acenaphthylene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Fluoranthene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Fluorene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Hexachlorobutadiene 2.8 U ug/L 2.8 4/5/2010 12:20 D-001 Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 4/5/2010 12:20 D-001 Priority Organic Pollutants Pyrene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(a)pyrene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(g,h,i)perylene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.8 U ug/L 2.8 4/5/2010 12:20 D-001 Priority Organic Pollutants Butyl benzyl phthalate 4.7 U ug/L 4.7 4/5/2010 12:20 D-001 Priority Organic Pollutants Di-n-butyl phthalate 4.7 U ug/L 4.7 4/5/2010 12:20 D-001 Priority Organic Pollutants Di-n-octyl phthalate 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.8 U ug/L 2.8 4/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dichlorophenol 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dimethylphenol 9.3 U ug/L 9.3 4/5/2010 12:20 D-001 Priority Organic Pollutants 2-Chloronaphthalene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Benzidine 93 U ug/L 93 
24 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remark Units MD

4/5/2010 12:20 D-001 Priority Organic Pollutants Bis(2-chloroethyl)ether 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 4/5/2010 12:20 D-001 Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 4/5/2010 12:20 D-001 Priority Organic Pollutants Phenol 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 2,6-Dinitrotoluene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 2-Nitrophenol 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 4/5/2010 12:20 D-001 Priority Organic Pollutants Acenaphthene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Anthracene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Chrysene 0.93 U ug/L 0.94/5/2010 12:20 D-001 Priority Organic Pollutants Hexachlorocyclopentadiene 2.8 U ug/L 2.8 4/5/2010 12:20 D-001 Priority Organic Pollutants Isophorone 0.93 U ug/L 0.94/5/2010 12:20 D-001 Field Parameter Temperature 34.34 C 4/5/2010 12:20 D-001 Field Parameter Sample Depth 0.1 m 4/5/2010 12:20 D-001 Field Parameter pH 7.7 4/5/2010 12:20 D-001 Field Parameter Specific Conductance 55580 umhos/cm 4/5/2010 12:10 Blank Metals Aluminum 60 U ug/L 60 4/5/2010 12:10 Blank Metals Cadmium 0.03 U ug/L 0.04/5/2010 12:10 Blank Metals Nickel 0.25 U ug/L 0.24/5/2010 12:10 Blank Metals Arsenic 0.25 U ug/L 0.24/5/2010 12:10 Blank Metals Zinc 5 U ug/L 5 4/5/2010 12:10 Blank Metals Magnesium 0.04 U mg/L 0.04/5/2010 12:10 Blank Metals Chromium 0.3 U ug/L 0.3 4/5/2010 12:10 Blank Metals Lead 0.2 U ug/L 0.2 4/5/2010 12:10 Blank Metals Copper 0.5 U ug/L 0.5 4/5/2010 12:10 Blank Metals Silver 0.025 U ug/L 0.04/5/2010 12:10 Blank Metals Calcium 0.075 U mg/L 0.04/5/2010 12:10 Blank Metals Selenium 0.2 U ug/L 0.2 4/5/2010 12:10 Blank Metals Iron 30 U ug/L 30 4/5/2010 12:10 Blank Nutrients Ammonia-N 0.01 U mg N/L 0.04/5/2010 12:10 Blank Nutrients O-Phosphate-P 0.004 U mg P/L 0.04/5/2010 12:10 Blank Nutrients NO2NO3-N 0.012 mg N/L 0.04/5/2010 12:10 Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 0.0
25 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remark Units MD

4/5/2010 12:10 Blank Nutrients Total-P 0.004 U mg P/L 0.04/5/2010 12:10 Blank Overflow Oil and Grease 1.5 I mg/L 1.4 4/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dimethylphenol 9.7 U ug/L 9.7 4/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dinitrophenol 15 U ug/L 15 4/5/2010 12:10 Blank Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants Benzo(a)pyrene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Bis(2-chloroethyl)ether 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants Butyl benzyl phthalate 4.9 U ug/L 4.9 4/5/2010 12:10 Blank Priority Organic Pollutants Chrysene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants Hexachlorobenzene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants Naphthalene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 4/5/2010 12:10 Blank Priority Organic Pollutants Phenol 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Chloro-3-methylphenol 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 4/5/2010 12:10 Blank Priority Organic Pollutants Di-n-butyl phthalate 4.9 U ug/L 4.9 4/5/2010 12:10 Blank Priority Organic Pollutants Dibenzo(a,h)anthracene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Diethyl phthalate 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 4/5/2010 12:10 Blank Priority Organic Pollutants Pentachlorophenol 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Acenaphthene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Anthracene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Benzidine 97 U ug/L 97 4/5/2010 12:10 Blank Priority Organic Pollutants Di-n-octyl phthalate 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Dimethyl phthalate 9.7 U ug/L 9.7 4/5/2010 12:10 Blank Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 4/5/2010 12:10 Blank Priority Organic Pollutants Phenanthrene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Pyrene 0.97 U ug/L 0.9
26 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remark Units MD

4/5/2010 12:10 Blank Priority Organic Pollutants Acenaphthylene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Benzo(b)fluoranthene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Benzo(k)fluoranthene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Isophorone 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 2,4,6-Trichlorophenol 0.97 U ug/L 0.9

4/5/2010 12:10 Blank Priority Organic Pollutants 2-Chloronaphthalene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 2-Chlorophenol 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 2-Nitrophenol 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 3,3'-Dichlorobenzidine 39 U ug/L 39 4/5/2010 12:10 Blank Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 15 UJ ug/L 15 4/5/2010 12:10 Blank Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 2.9 4/5/2010 12:10 Blank Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Nitrophenol 15 U ug/L 15 4/5/2010 12:10 Blank Priority Organic Pollutants Benzo(g,h,i)perylene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Fluoranthene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Fluorene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dichlorophenol 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dinitrotoluene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants 2,6-Dinitrotoluene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Benzo(a)anthracene 0.97 U ug/L 0.94/5/2010 12:10 Blank Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.97 U ug/L 0.94/5/2010 12:10 Blank Field Parameter Sample Depth 0.1 m 
27 

Appendix F. Facility Summary, Effluent Limits, and Permit Violations 

Recorded on Discharge Monitoring Report 
28 

Appendix F. (continued) 
29 

Appendix F. (continued) 
30 

Appendix F. (continued) 
31 

Appendix F. (continued) 
32 

Appendix F. (continued) 
33 

Appendix G. EPA End Sheet 

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 2 0 8 11 12 1 0 0 4 0 5 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 2 0 8 11 12 1 0 0 4 0 5 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 2 0 8 11 12 1 0 0 4 0 5 18 S 19 S 20 2

66

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of FPL- St. Lucie Plant effluent sampled on April 5, 

2010. NPDES #FL0002208.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of FPL-St. Lucie Plant effluent 

sampled on April 5, 2010. NPDES #FL0002208.

Biological Analyses of FPL-St. Lucie Plant effluent sampled on 

April 5, 2010. NPDES #FL0002208.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)