### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 1 of 26 **UNITED STA**

**TES ENVIRONMENTA L PROTECTION A**  **GENCY WA** **SHINGTON, D.C. 20460 Office of Chem**  **ical Safety and Pollution Prevention**
**ME MORANDUM**  

## February 23 , 201 2
**SUBJECT: Acibenzolar**  **- S**  **- Methyl** 

**–**Acute and Chronic Dietary and Drinking Water

Exposure and Risk Assessment to support a Section 3 Request for Use of Acibenzolar
- S
- Methyl on Lo w Growing Berries Crop Subgroup 13
- 07G
. **PC Code:** 06140 2 **DP Barcode:** 38 7069 **Decision No.:** 4 43561 **Registration No. :**  100
- 922 **Petition No.:** PP# 0E7818 **Regulatory Action:** Section 3 **Risk Assessment Type:** Dietary **Case No.:** NA **T XR No.:**  NA **CAS No.:** 135158
- 54
- 2 **MRID No.:** NA **40 CFR:** 180.561 

## FROM: Thurston Morton, Chemist

Risk Assessment Branch IV Health Effects Division (7509 P ) **THROUGH:** Mohsen Sahafeyan , Chemist

Am elia Acierto, Chemist Dietary Exposure Science Advisory Council (DESAC), Susan Hummel, Chemist Elissa Reaves PhD, Branch Chief Risk Assessment Branch IV Health Effects Division (7509P) **TO:** Becky

Daiss, Risk Assessor Risk Assessment Branch 4 Health E ffects Division (7509P)

And Sidney Jackson (RM 05) Risk Integration, Minor Use & Emergency Response Branch Registration Division (7505P)

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 2 of 26 This document provides a revised acute and chronic dietary and drinking water risk analy ses of exposure s re sulting from use of acibenzolar
- S
- methyl as a plant activator on food crops . This assessment supports a Section 3 request for use of acibenzolar
- S
- methyl on low growing berries Crop Subgroup 13
- 07G
. **Executive Summary** Acute and chronic dietary r isk assessments were conducted for acibenzolar
- S
- methyl using the Dietary Exposure Evaluation Model (DEEM
- FCI D, Version 2.03), which uses food consumption data from the USDA’s Continuing Survey s of Food Intakes by Individuals (CSFI I) from 1994
- 1996 and 199 8. The analy ses were performed to support a Section 3 request for use of acibenzolar
- S
- methyl on the low growing berries Crop Subgroup 13
- 07G
. Acute Dietary

Exposure Results and Characterization A refined (probabilistic) acute dietary exposure analy s is was performed for the general population and all population subgroup s. The acute analy sis assumed a distribution of residues based on field trial data. Empirical and DEEM default processing factors were used to modify the field trial data. M aximum sc reening
- level percent crop treated estimates were used for commodities for which data were available. I f no percent crop treated data were available, 100% crop treated was assumed. The acute analy sis incorporated the 1 in 10 year peak surface drinking water estimate f

rom application of acibenzolar
- S
- methyl to cucurbit vegetables. Acute dietary risk estimates are not of concern for general population or other population subgroups. The subgroup with the highest risk estimate was children 3
- 5 years old with a 99.9 th 

## percentile acute exposure estimate of 3 5 % of the aPAD. The 99.9 th 

## percentile aPAD for the general U.S. population was 1 7 %. Chronic Dietary

Exposure Results and Characterization A conservative chronic dietary exposure analy sis was performed fo r the general U.S. population and various population subgroups. Tolerance level residues and 100 crop treated assumptions were used. DEEM default and empirical processing factors were used to modify the tolerance values. The chronic analy sis incorporate d the 10 year average surface drinking water estimate from application of acibenzolar
- S
- methyl to cucurbit vegetables. The population subgroups females 13
- 49 and young children had a more protective chronic population adjusted dose (cPAD, 0.082 mg/kg/day

) than the general U.S. population and all other population subgroups (0.25 mg/kg/day

). Chronic dietary

risk estimates are not of concern for general population or other population subgroups. The subgroups with the highest risk estimate were children 1
- 2 and 3
- 5 years old with a cPAD of 11%. The cPAD for the general U.S. population was 5 %.

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 3 of 26 **I. Introduction**  Dietary risk assessment incorporates both exposure and toxicity of a given pesticide. For acute and chronic assessments, the risk is expressed as a percentage of a maximum acceptable dose (i.e., the dose which HED has concluded will result in no unreasonable adverse health effects). This dose is referred to as the population adjusted dose (PAD). The PAD is equivalent to the Reference Dose (RfD) d ivided by the FQPA Safety

Factor. For acute and non
- cancer chronic exposures, HED is concerned when estimated dietary risk exceeds 100 % of the PAD. References that discuss the acute and chronic risk assessments in more detail are available on the EPA/pes ticides web site: “Available Information on Assessing Exposure from Pesticides, A User’s Guide,” 6/21/2000, web link: http://www.epa.gov/fedrgstr/EPA_PEST/2000/July /Day _12/606 1.pdf ; or see SOP 99.6 (8/20/99). **II. Residue Information**  Residues of Concern The HED Metabolism Assessment Review Committee (MARC) reviewed lettuce, tomato, cotton, lemon, poultry , and ruminant metabolism studies and the environmental fate data. Ta ble 1 provides a summary

of the MARC decisions regarding residues of concern for acibenzolar
- S
methyl
. (D261037, M. Doherty , 4/26/00) **Table 1**  **: Summ**  **ary of MARC D**  **ecisions for Acibenzolar**  **- S**  **- me**  **thyl Matrix**  **Residues of Concern 1**  

### For Risk Assessm **ent 2**  

### Fo **r Tolerance Expression** Plants Residues of acibenzolar
- S
- methyl, benzo(1,2,3)

thiadiazole
- 7
- carbothioic acid
- S
- methyl ester, convertible to benzo(1,2,3)thiadiazole
- 7
- carboxylic acid (CGA
- 210007), expressed as acibenzolar
- S
- methyl & 4
- hydroxy CGA
- 210007 (CG A
- 323060) 5
- hydroxy CGA
- 210007 (CGA
- 324041) R esidues of acibenzolar
- S
- methyl, b enzo(1,2,3) thiadiazole
- 7
- carbothioic acid
- S
- methyl ester, convertible to benzo(1,2,3)thiadiazole
- 7
- carboxylic acid (CGA
- 210007), expressed as acibenzolar
- S
- methyl 1 The residues of concern for drinking water are the same as those listed in the tolerance expression 2 If a future petition is received for rice, residues of CGA
- 379019 should be included in the risk assessment

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 4 of 26 Established/Recommended Tolerances Tolerances have been established (40CFR 180.561) for residues of acibenzolar
- S
- methyl (benzo(1,2,3)thiadiazole
- 7
- carbothioic acid
- S
- methyl ester) in/on spinach at 1.0 ppm, leafy vegetables (Crop Group 4, other than spinach, at 0.25 ppm), Brassica (cole) leafy veget ables (Crop Group 5 at 1.0 ppm), fruiting vegetables (Crop Group 8 at 1.0 ppm), and tomato paste at 3.0 ppm. There is also an import tolerance for bananas at 0.1 ppm. Tolerances have been recently established for the bulb onion subgroup 3
- 07A at 0.1 ppm and the cucurbit vegetable crop group 9 at 2.0 ppm
. HED is recommending for a tolerance of 0.15 ppm for residues of acibenzolar
- S
- methyl in/on the low growing berry subgroup 13
- 07G. The tolerance expression for acibenzolar
- S
- methyl is for residues co nvertible to benzo(1,2,3)thiadiazole
- 7
- carboxylic acid (CGA
- 210007), expressed as acibenzolar
- S
- methyl
. Residues U sed in the Chronic Analy sis Inthe chronic dietary

exposure analy sis, tolerance level residues were used and 100 percent crop treated was assum ed f

or all commodities. DEEM default processing factors were used for dried onion, dried banana, dried plantain, and dried tomato. A processing factor was not used for tomato paste because a separate tolerance has been established for this processed commodity . In the submitted tomato processing study (D246673, M. Doherty , 3/15/2000), reported processing factors of 1.0 and 2.9 for tomato juice and tomato puree were used
. Available lettuce metabolism data for a 7
- day PHIwere used to determine th e relative abundance of the hydroxy

metabolites (CGA
- 323060 and CGA
- 324041) and residues convertible to the carboxylic acid metabolite (CGA
- 210007). Based on the lettuce metabolism data, a factor of 1.5x was applied to estimate s of acibenzolar
- S
- methyl re sidues to account for all of the residues of concern for dietary

risk (including CGA
- 210007 , CGA
- 323060 and CGA
- 324041 )
. Lettuce metabolism data are provided in Table 2.

**Table 2. Lettuce metabolism data (Washed, 7 -**  **day PHI)**

**Com pound**  **% Residues** Aciben zolar
- S
- Methyl 17 CGA
- 210007 25 CGA
- 323060 20 CGA
- 324041 0.06 

## Residues Used in the Acute Analy sis A probabilistic assessment was performed f or the acute analy sis
. The acute analy sis assumed a distribution of residues based on field trial data for n onblended and partially blended commodities. For blended commodities, the mean field trial values were used as a point estimate. A value of 1⁄2 L OQ was used for samples that contained less than L OQ residues. Foods

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 5 of 26 were classified as blended, partially blen ded, or nonblended based on guidance given in the Draft DEEM
- FCI D Classification of Food Forms with Respect to Blending (F. Fort, 4/9/2003). DEEM default processing factors were used for dried onion, dried banana, dried plantain, and dried tomato. Empiri cal processing factors were used for tomato paste (7.1), tomato puree (2.9), and tomato juice (1.0). The acute analy sis used available maximum percent crop treated estimates from the Biological and Economic Analy sis Division ’s (BEAD ’s ) screening
- level ana ly sis (D353541, J. Alsadek, 6/18/08). I f no percent crop treated data were available 100% crop treated was assumed. As previously noted, a factor of 1.5 X was applied to the estimate of residues convertible to CGA
- 210007 for all commodities to account for all of the resid ues of concern for dietary risk. Residues used in the acute and chronic dietary analy ses are provided in Table 3.

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 6 of 26 **Table 3**  **. Data and Residue Estimates Used in Acibenzolar**  **- S**  **- Methyl**  **Dietary and Drinking Water**  **Analyses**
**Com modity**  **Blende d**  **Status 1**  **Data**

**Source %CT**  **Process Factors**  **Anticipated Residue**

**Estim ates/Tolerance**  **2 Avg**  **Max Acute**  **Chronic (Tolerance)**  **Crop Group 3 -**  **07 A**  **Onions** Onion, dry bulb and dry bulb
- babyfood NB/PB Field Trials 14 samples 1 detectable residue MRID 47375301 10 0 100 NA RDF# 23 Onion (100% CT) TOTAL Z=0 TOTAL NZ=7 0.1 Onion , dry

bulb dried, and dried
babyfood B 9 Tolerance 0.1 Onion , Green Onion NA **Crop Group 4A**

**– Leafy Greens**  Amaranth , Arugula , Cress C hry santhemum , Endive , Dandelions , Parsley PB F ield Trails 11 samples 11 detectable residues MRID 44537062 100 100 NA RDF# 18 Spinich Oth (100% CT) TOTAL Z=0 TOTAL NZ=11 0.25
Le ttuce, head NB/PB Field Trails 12 samples 12 detectable residues MRID 44537062 5 15 NA RDF# 6 Le ttuceH (12% CT) T OTAL Z=88 TOTAL NZ=12 0.25
Le ttuce, leaf PB Field Trails 11 samples 11 detectable residues MRID 44537062 5 15 NA RDF# 8 Le ttuceL (12% CT) TOTAL Z=81 TOTAL NZ=11 0.25 Radicchio PB Field Trails 12 samples 12 detectable residues MRID 44537 062 100 100 NA RDF# 16 Le ttuceHRad (12% CT) TOTAL Z=0 TOTAL NZ=12 0.25 Spinach PB Field Trails 11 samples 11 detectable residues MRID 44537062 25 40 NA RDF# 10 Spinach ( 30 % CT) TOTAL Z= 26 TOTAL NZ=11 1.0 **Crop Group 4B**

**– Leafy Petioles**  Cardoon Celtuce Fenne l Rhubar b Swiss char d NB Field Trails 9 samples 9 detectable residues MRID 44537062 100 100 NA RDF# 15 CeleryOth (1 00 % CT) TOTAL Z=0 TOTAL NZ=9 0.25 Celery NB/PB Field Trails 9 samples 9 detectable residues MRID 44828007 1 00 1 00 NA RDF# 15 Celery (1 00 % CT ) TOTAL Z=0 TOTAL NZ=9 0.25 **Crop Group 5A**

**– Brassica Head & Stem**  Broccoli Cauliflower NB/PB Field Trails 11 samples 11 detectable residues MRID 44828007 5 5 5

10 NA RDF# 3 Broccoli ( 5 % CT) TOTAL Z= 209 TOTAL NZ= 11 1.0

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 7 of 26 **Table 3**  **. Data and Residue Estimates Used in Acibenzolar**  **- S**  **- Methyl**  **Dietary and Drinking Water**  **Analyses**
**Com modity**  **Blende d**  **Status 1**  **Data**

**Source %CT**  **Process Factors**  **Anticipated Residue**

**Estim ates/Tolerance**  **2 Avg**  **Max Acute**  **Chronic (Tolerance)**  Cabbage NB/PB Field Trails 11 samples 11 detectable residues MRID 44828007 1 1 NA RDF# 4 Cabbage ( 2.5 % CT) TOTAL Z= 429 TOTAL NZ=11 1.0 Cabbage Chinese Mustard Brocolli Chinese NB Field Trails 11 samples 11 detectable residues MRID 44828007 100 100 NA RDF# 13 Broccoli Oth (100% CT) TOTAL Z=0 TOTAL NZ=11 1.0 Kohlrabi Brussels sprouts Cabbage Chinese N apa NB Field Trails 11 samples 11 detectable residues MRID 44828007 100 100 NA RDF# 14 Cabbage Oth ( 100 % CT) TOTAL Z= 0 TOTAL NZ=11 1.0 **Crop Group 5 B** 

**– Brassica Leafy Greens**  Cabbage Chinese bok choy Collards Kale Mustard Greens Rape greens Turnip greens NB Field Trails 9 samples 9 detectable residues MRID 44828007 100 100 NA RDF# 9 Mustard ( 100 % CT) TOTAL Z= 0 TOTAL NZ= 9 1.0 **Crop Group 8**

**– Fruiting Vegetables**  Eggplant, NB/PB Field Trails 9 sampl es 9 detectable residues MRID 44537060 100 100 NA RDF# 12 BellPepOth ( 100 % CT) TOTAL Z= 0 TOTAL NZ= 9 1.0 Okra NB/PB Field Trails 9 samples 9 detectable residues MRID 44537060 100 100 NA RDF# 17 HotPepOth ( 100 % CT) TOTAL Z= 0 TOTAL NZ= 9 1.0 Pepper nonbell NB /PB Field Trails 5 samples 5 detectable residues MRID 44537060 5 10 NA RDF# 7 HotPep ( 5 % CT) TOTAL Z= 95 TOTAL NZ= 5 1.0 Pepper nonbell , dried B Field Trails 5 samples 5 detectable residues MRID 44537060 5 10 NA Mean field trial (0.321) 1.0 Pepper, bell NB/PB Field Trails 9 samples 9 detectable residues MRID 44537060 5 10 NA RDF# 2 BellPep ( 5 % CT) TOTAL Z= 179 TOTAL NZ= 9 1.0 Tomatillo NB/PB Field Trails 23 samples 23 detectable residues MRID 44537060 2.5 5 NA RDF# 19 TomatoOth ( 100 % CT) TOTAL Z= 0 TOTAL NZ= 23 1.0

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 8 of 26 **Table 3**  **. Data and Residue Estimates Used in Acibenzolar**  **- S**  **- Methyl**  **Dietary and Drinking Water**  **Analyses**
**Com modity**  **Blende d**  **Status 1**  **Data**

**Source %CT**  **Process Factors**  **Anticipated Residue**

**Estim ates/Tolerance**  **2 Avg**  **Max Acute**  **Chronic (Tolerance)**  Tomato NB/PB Field Trails 23 samples 23 detectable residues MRID 44537060 2.5 5 NA RDF# 11 Tomato ( 5 % CT) TOTAL Z= 437 TOTAL NZ= 23 1.0 Tomato, paste, B Field Trails 23 samples 23 detectable residues MRID 44537060 2.5 5 7.1 Mean field trial: 0.164 1.0 Tomato, puree B Field Trails 23 samples 23 detectable residues MRID 44537060 2. 5 5 2.9 Mean field trial: 0.164 1.0 Tomato, dried B Field Trails 23 samples 23 detectable residues MRID 44537060 2. 5 5 14.3 Mean field trial: 0.164 1. 0 **Crop Group 9A** 

**– Cu**  **curbit Vegetables -**  **Melon** Cantaloupe , Casaba , Honeydew melon , Watermelon NB/PB Field Trails 12 samples 12 detectable residues MRID 45105301 100 100 NA RDF# 20 Cantaloupe ( 100 % CT) TOTAL Z= 0 TOTAL NZ= 6 2.0 **Crop Gro up 9B** 

**– Cucurbit Vegetables** 

**– Squash Cucumbers**  Chayote, fruit, Chinese waxgourd NB NA 2.0 Cucumber , Balsam pear NB Field Trails 16 samples 16 detectable residues MRID 45105301 100 100 NA RDF# 21 Cucumber ( 100 % CT) TOTAL Z= 0 TOTAL NZ=8 2.0 Pumpkin, Squash NB/PB Field Trails 10 samples 10 detectable residues MRID 45105301 100 100 NA RDF# 22 Squash (100% CT) TOTAL Z=0 TOTAL NZ=5 2.0 **Crop Group O -**  **Other** Banana, Plantain NB/PB Field Trails 30 samples 8 detectable residues MRID 44828004 100 100 NA RDF#1 Banana(100%CT) Total Z=0 TotalNZ=30 0.1 Banana, Plantain, dried B Field Trails 30 samples 8 detectable residues MRID 44828004 100 100 3.9 Mean field trial (0.0107) 0.1
Water NA D 351609 100 100 NA 0.01 7 0.011

1 Classification o f blended (B), partially blended (PB), not blended (NB). 2 A factor of 1.5X was applied to estimate residues convertible to CGA
- 210007 for all commodities to account for all of the residues of concern for dietary risk.

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 9 of 26 **III. Water Data**  Drinking water re sidues provided by the Environmental Fate and Effects Division (EFED) (D35 3745 , R. Miller, 9 / 2 /10) were incorporated directly into the acute and chronic dietary analy ses . T he surface water estimates were based on EFED’s Tier I I model Pesticide Root Zone Model
. The residues of concern for drinking water include acibenzolar
- S
- methyl and residues convertible to CGA
- 210007. The estimated EDWCs for use of acibenzolar
- S
- methyl on cucumbers were used for the drinking water assessment becau se this use represents the highest use rate of the existing and proposed registered uses. The SCI
- GROW estimated ground water drinking water concentrations for acibenzolar is not expected to exceed 1.25 x 10 - 5 

## μg/L and CGA 210007 is not expected to exceed 8.12 x 10 - 2 

## μg/L. Surface water EDWCs for acibenzolar
S
- methyl, which were used in the exposure assessment, are provided in Table 4 . CGA
- 210007 drinking water residues were included in the dietary exposure assessment as acibenzolar
- S
methyl equivalents.

**Table 4**  **. EDWCs for use in screening the hum an health risk assessm**  **ent ( FL Cucum**  **ber )**  **Drinking Water Source**  **Chem ical**  **1 -**  **in -**  **10 year annual acute**  **conc. (μg/L)**  **1 -**  **in -**  **10 year avg**

**annual (chronic) concentration**  **(μg/L) 30**  **- year avg. annual** 

**concentration (μg/L)**  Surface Water Acibenzolar
- S
- Methyl 1 

### 0.7 2 0. 02 0.0 2 Surface Water CGA 210007 2 

### 20.02 8.09 4.68 1 Aerial application method 2 Degradation product formed after application 

## CGA
- 210007 drinking water residues were included in the dietary exposure assessment as acibenzolar
- S
- methyl equivalents. CGA 210007 residues were converted based on molecular weight as follows. Acute: The acute time distribution was adjusted to account for the metabolite. Chronic: 210 ÷ 180 x 8.09 (μg/L) = 9.44 μg/L CGA 210007 

## IV. **DEEM -**  **FCIDTM Program and Consumption Information** Acibenzolar
- S
- Methyl acute and chronic dietary exposure assessments were conducted using the Dietary Exposure Evaluation Model software with the Food Commodity Intake Data

base (DEEM
- FCI DTM, Version 2.03), which incorporates consumption data from USDA’s Continuing Survey s of Food Intakes by Individuals (CSFI I), 1994
- 1996 and 1998. The 1994
- 96, 98 data are based on the reported consumption of more than 20,000 individuals over two non - consecutive survey day s. Foods “as consumed” (e.g., apple pie) are linked to EPA
- defined food commodities (e.g. apples, peeled fruit
- cooked; fresh or N/S; baked; or wheat flour
- cooked; fresh or N/S, baked) using publicly

available recipe tran slation files developed jointly by USDA/ARS and EPA. For chronic exposure assessment, consumption data are averaged for the entire U.S. population and within population subgroups, but for acute exposure assessment are retained as individual consumption ev ents. Based on analy sis of the 1994
- 96, 98 CSFI I consumption data, which took into account dietary patterns and survey

respondents, HED concluded that it is most

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 10 of 26 appropriate to report risk for the following population subgroups: the general U.S. populatio n, all infants (<1 year old), children 1
- 2, children 3
- 5, children 6
- 12, youth 13
- 19, adults 20
- 49, females 13
- 49, and adults 50+ years old. For chronic dietary

exposure assessment, an estimate of the residue level in each food or food
form (e.g., orange or orange juice) on the food commodity residue list is multiplied by the average daily

consumption estimate for that food/food form. The resulting residue consumption estimate for each food/food form is summed with the residue consumption estimates for al l other food/food forms on the commodity residue list to arrive at the total average estimated exposure. Exposure is expressed in mg/kg body weight/day

and as a percent of the cPAD. This procedure is performed for each population subgroup. For acute exp osure assessments, individual one
- day food consumption data are used on an individual
- by
- individual basis. The reported consumption amounts of each food item can be multiplied by

a residue point estimate and summed to obtain a total daily pesticide exposure for a deterministic exposure assessment, or “matched” in multiple random pairings with residue values and then summed in a probabilistic assessment. The resulting distribution of exposures is expressed as a percentage of the aPAD on both a user (i.e., those who reported eating relevant commodities/food forms) and a per
- capita (i.e., those who reported eating the relevant commodities as well as those who did not) basis. In accordance with HED policy , per capita exposure and risk are reported for all tie rs of analy sis. However, for tiers 1 and 2, significant differences in user vs. per capita exposure and risk are identified and noted in the risk assessment. **V. Toxicological Information**  HED evaluated the toxicology data base for a cibenzolar
- S
- methyl an d selected the following doses and endpoints for acute and chronic dietary and drinking water risk assessments. [Note: In the previous dietary exposure assessment risk assessment (D36 2208 , 3/3/09), only the subpopulation females 13
- 50 years old was asse ssed for acute dietary exposure based on in utero effects. Additionally , only the subpopulation females 13
- 50 years old was assessed for chronic dietary exposure based on in utero effects observed in the DNT study
. However, based on a reevaluation of the DNT study regarding the potential for post
- natal toxicity , the acute dietary analy sis has been expanded to include children for this assessment and the the chronic dietary analy sis has been revised to include an assessment of dietary risks to children u sing the NOAEL from the DNT study .]

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 11 of 26 **Table 5**  **. Sum ma**  **ry of Toxicological Doses and Endpoints for Acibenzolar -**  **S -**  **me thyl for Use in Dietary and Non**  **- Occupational**  **Hum an Health Risk Assessm**  **ents Exposure/Scenario**  **Point of Departure**  **Uncertainty/FQPA**

**Safety F actors**  **RfD, PAD, LOC for Risk**  **Assessment Study and Toxicological Effects** 

Acute Dietary (General Population) NOAEL = 8.2 mg/kg/day 

### UF A

### = 10x

UF H 

### =10x FQPA SF= 1x Acute RfD =

0.082 mg/kg/day aPAD = 0.082 mg/kg/day Developmental Neurotoxicity Toxicity Study
- Rat

Developmental LOAEL = 82 mg/kg/day based on changes in brain morphometrics in the cerebellum in offspring. Maternal LOAEL = was not observed NOAEL = 4000 mg/kg/day HDT 

### Chronic Dietary (Females 13
- 49 years & Young Children) NOAEL = 8.2 mg/kg/day 

### UF A

### = 10x

UF H 

### =10x FQPA SF= 1x Acute RfD =

0.082 mg/kg/day c PAD = 0.082 mg/kg/day Developmental Neurotoxicity Toxicity Study
- Rat

Developmental LOAEL = 82 mg/kg/day based on changes in brain morphometrics in the cerebellum in offspring. Maternal LOAEL = was not observed NOAEL = 4000 mg/kg/day HDT 

### Chronic Dietary (Adult Males and Females 50+ yrs) NOAEL = 25 mg/kg/day 

### UF A

### = 10 x

UF H 

### =10x FQPA SF= 1x Chronic RfD =

0.0 25 mg/kg/day cPAD = 0. 25 mg/kg/day Chronic Toxicity
- Dog; Co
- critical; Chronic/Cancer
-

R at & Mouse, Reproduction Toxicity
- Rat LOAEL = 25 mg/kg/day based on hemolytic anemia with compensatory response. 

### Cancer ( all routes ) Not a Human Carcinogen

Point of Departure (POD) = A data point or an estimated point that is derived from observed d ose - response data and used to mark the beginning of extrapolation to determine risk associated with lower environmentally relevant human exposures. NOAEL = no observed adverse effect level. NOAEL = no observed adverse effect level. LO AEL = lowest obs erved adverse effect level. UF = uncertainty factor. UF A = extrapolation from animal to human (interspecies). UF H = potential variation in sensitivity among members of the human population (intraspecies). FQPA SF = FQPA Safety Factor. PAD = popul ation adjusted dose (a = acute, c = chronic). RfD = reference dose. 

## VI. **Results/Discussion** As stated above, for acute and chronic assessments, HED is concerned when dietary risk exceeds 100% of the PAD. The DEEM
- FCI DTM analy ses estimate the dietary exposure of the U.S. population and various population subgroups. The results reported in Tables 6
- 8 are for the general U.S. Population, all infants (<1 year old), children 1
- 2, children 3
- 5, children 6
- 12, youth 13
- 19, females 13
- 49, adults 20
- 49, and adults 50+ years. Results of Acute Dietary

Exposure Analy sis The results of the acute dietary exposure analy sis at the 99.9 th 

## percentile of exposure are reported in Table 6
.

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 12 of 26 **Table 6**  **: Summary of Acut e Dietary Exposure and Risk**  **(Food and Water) for Acibenzolar**  **- S**  **** **Methyl Population Subgroup**  **aPAD (mg/kg/day) Acute (99**  **.9 th**  

### Percentile) **Exposure (mg/kg/day) % aPAD**  General U.S. Population

0.082 0.014 120 1 7 All Infants (< 1 year old) 0.0 1 3034 1 6 Childr en 1
- 2 y ears old 0.0250 51 3 1 Children 3
- 5 y ears old 0.0 28 600 3 5 Children 6
- 12 y ears old 0.0 199 31 2 4 Youth 13
- 19 y ears old 0.015 0 67 1 8 Adults 20
- 49 y ears old 0.009 0 85 11 Adults 50+ y ears old 0.01 06 51 1 3 Females 13
- 49 y ears old 0.009 5 61 12 

## Res ults of Chronic Dietary Exposure Analy sis The results of the chronic dietary exposure analy sis are reported in T able s 7 and 8
. **Table 7**  **: Summary of Chronic Dietary Exposure and Risk to Female and Child Population for Acibenzolar**  **- S**  **- Methyl**  **Population S ubgroup**  **cPAD (mg/kg/day) Chronic**  **Exposure (mg/kg/day) % cPAD**  Females 13
- 49 y ears old 0.082 0.003 646 4 All Infants (< 1 year old) 0.0038 08 5 Children 1
- 2 y ears old 0.009 207 11 Children 3
- 5 y ears old 0.00 8706 11 Children 6
- 12 y ears old 0.00 5812 7 **Table 8**  **: Summary of Chronic Dietary Exposure and Risk to Adults 50+ and Adult Males (13+) Acibenzolar**  **- S**  **- Methyl**  **Population Subgroup cPAD (mg/kg/day)**  **Chronic Exposure (mg/kg/day)**  **% cPAD** General U.S. Population 0.25 0.004 366 5 Youth 13
- 19 0.00 3893 2 Adults 20
- 49 0.003 654 2 Adults 50+ y ears old 0.00 3852 2 

## VI. **Characterization of Inputs/Outputs** The chronic dietary

analy sis is very

conservative. T olerance level residues used in the analy sis are expected to exceed the residues actually consumed . In addition, the 100% crop treated estimate used for all crops in the assessment will considerably overestimate the actual percent crop treated.

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 13 of 26 The acute dietary

analy sis is refined
. The use of field trial data and BEAD’s maximum percent crop treat ed estimates in a probabilistic assessment provided exposure estimates that are more likely to be representative of actual exposures. These estimates could be further refined, however by use of percent crop treated estimates for the crops for which 100% c rop treated assumptions were made , use of monitoring data, market basket survey s, washing factors, and cooking factors
. To further characterize the exposure of acibenzolar
- s - methyl residues, HED conducted a CSA. The purpose of the CSA calculations is to inform risk managers on the range of exposures that an individual may

obtain from eating a treated commodity . For acibenzolar
- s - methyl, HED estimated the risk to a 15 kg toddler consuming a reasonable serving of strawberry or blueberry with residues at the tolerance . The resulting risk estimates were 2 % and 1 % of the aPAD for strawberry and blueberry , respectively
. **VII. Conclusions**  A conservative chronic dietary exposure analy sis was performed for the general U.S. population and various population subgroups. The risk estimates for the general U.S. population and all population subgroups are below HED’s level of concern. HED is confident that acute and chronic dietary

exposures to acibenzolar
- S
- methyl are not underestimated. **VIII. List of Attachments**  1. Acute DEEM
- FCI D Residue Input File. 2. Results of Acute DEEM
- FCI D Dietary Exposure Analy sis. 3. Chronic DEEM
- FCI D Residue Input File. 4. Results of Chronic DEEM
- FCI D Dietary Exposure Analy sis. 5. Percent Crop Treated Estimates Provided by BEAD 6. RDF Files Used in Acute Analy sis

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 14 of 26 Attachment 1 Acute DEEM
- FCI D Residue Input File
Filename: C: \ Documents and Settings \ tmorton \ My Documents \ MyFiles \ DEEM Files \ 061402 Acibenzolar \ Acibenzolar on Low Growing Berries \ 061402Acute.R98 Chemical: Acibenzolar
- S - M ethyl RfD(Chronic): .082 mg/kg bw/day NOEL(Chronic): 0 mg/kg bw/day RfD(Acute): .082 mg/kg bw/day NOEL(Acute): 0 mg/kg bw/day Date created/last modified: 06
- 24
- 2011/15:03:28/8 Program ver. 2.03

-------------------------------------------------- ------------------------------ RDL indices and parameters for Monte Carlo Analysis: Index Dist Parameter #1 Param #2 Param #3 Comment # Code

----- ---- ------------ -------- -------- ------- 1 6 Banana.rdf 2 6 BellPep.r df 3 6 Broccoli.rdf 4 6 Cabbage.rdf 5 6 Celery.rdf 6 6 LettuceH.rdf 7 6 HotPep.rdf 8 6 LettuceL.rdf 9 6 Mustard.rdf 10 6 Spinach.rdf 11 6 Tomato.rdf 12 6 BellPepOth.rdf 13 6 BroccoliO th.rdf 14 6 CabbageOth.rdf 15 6 CeleryOth.rdf 16 6 LettuceHRad.rdf 17 6 HotPepOth.rdf 18 6 SpinachOth.rdf 19 6 TomatoOth.rdf 20 6 Cantaloupe.rdf 21 6 Cucumber.rdf 22 6 Squash.rdf 23 6 Onion.rdf 24 6 Low Growing Berries.rdf 25 6 Cauliflower.rdf 26 6 DrinkingWaterAcibenzolarPlusConvertedMetabolite.rdf EPA Crop Commodity Name Def Res Adj.Factors RDL Comment Code Grp (ppm) #1 #2 Pntr

-------- ---- ------------------------------- ---------- ------ ------ --- ------- 03002370 3 Onion, dry bulb 0.100000 1.000 1.500 23 03002371 3 Onion, dry bulb
- babyfood 0.100000 1.000 1.500 23 03002380 3 Onion, dry bulb, dried 0.029000 9.000 1.500 03002381 3 Onion, dry bulb, dried
- babyfood 0.029000 9.000 1.500 03002390 3 Onion, green 0.100000 1.000 1.500 23 03003380 3 Shallot 0.100000 1.000 1.500 23 04010050 4A Amaranth, leafy 0.250000 1.000 1.500 18 04010180 4A Arugula 0.250000 1.000 1.500 18 04011040 4A Chry santhemum, garland 0.250000 1.000 1.500 18 04011330 4A Cress, garden 0.250000 1.000 1.500 18 04011340 4A Cress, upland 0.250000 1.000 1.500 18 04011380 4A Dandelion, leaves 0.250000 1.000 1.500 18 04011500 4A Endive 0.250000 1.000 1.500 18 04012040 4A Lettuce, head 0.250000 1.000 1.500 6 04012050 4A Lettuce, leaf 0.25000 0 1.000 1.500 8 04012480 4A Parsley, leaves 0.250000 1.000 1.500 18 04013130 4A Radicchio 0.250000 1.000 1.500 16 04013550 4A Spinach 1.000000 1.000 1.500 1 0 04013551 4A Spinach
- babyfood 1.000000 1.000 1.500 10 04020760 4B Cardoon 0.250000 1.000 1.500 15 04020850 4B Celery 0.250000 1.000 1.500 5 04020851 4B C elery - babyfood 0.250000 1.000 1.500 5 04020860 4B Celery, juice 0.250000 1.000 1.500 5 04020870 4B Celtuce 0.250000 1.000 1.500 15 04021520 4B Fennel, Florence 0.250000 1.000 1.500 15 04023220 4B Rhubarb 0.250000 1.000 1.500 15 04023670 4B Swiss chard 0.250000 1.000 1.500 15

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 15 of 26 05010610 5A Broccoli 1.00 0000 1.000 1.500 3 05010611 5A Broccoli
- babyfood 1.000000 1.000 1.500 3 05010620 5A Broccoli, Chinese 1.000000 1.000 1.500 13 05010640 5A Brussels sprouts 1.000000 1.000 1.500 14 05010690 5A Cabbage 1.000000 1.000 1.500 4 05010710 5A Cabbage, Chinese, napa 1.000000 1.000 1.500 14 05010720 5A Cabbage, Chinese, mustard 1.000000 1.000 1.500 13 05010830 5A Cauliflower 1.000000 1.000 1.500 25 05011960 5A Kohlrabi 1.000000 1.000 1.500 14 05020630 5B Broccoli raab 1.000000 1.000 1.500 9 05020700 5B Cabbage, Chinese, bok choy 1.000000 1.000 1.500 9 05021170 5B Collards 1.000000 1.000 1.500 9 05021940 5B Kale 1.000000 1.000 1.500 9 05022290 5B Mustard greens 1 .000000 1.000 1.500 9 05023180 5B Rape greens 1.000000 1.000 1.500 9 05023890 5B Turnip, greens 1.000000 1.000 1.500 9 08001480 8 Eggplant 1.000000 1.000 1. 500 12 08002340 8 Okra 1.000000 1.000 1.500 17 08002700 8 Pepper, bell 1.000000 1.000 1.500 2 08002701 8 Pepper, bell
- babyfood 1.000000 1.000 1.500 2 08002710 8 Pepper, bell, dried 0.321000 1.000 0.075 1.5 x Full comment: 1.5 x 5%CT 08002711 8 Pepper, bell, dried
- babyfood 0.321000 1.000 0.075 1.5 x Full comment: 1.5 x 5%CT 08002720 8 Pepper, nonbell 1.000000 1.000 1.500 7 08002721 8 Pepper, nonbell
- babyfood 1.000000 1.000 1.500 7 08002730 8 Pepper, nonbell, dried 0.358000 1.000 0.075 1.5 x Full comment: 1.5 x 5%CT 08003740 8 Tomatil lo 1.000000 1.000 1.500 19 08003750 8 Tomato 1.000000 1.000 1.500 11 08003751 8 Tomato
- babyfood 1.000000 1.000 1.500 11 08003760 8 Tomato, paste 0.164000 7.100 0.075 1.5 x Full comment: 1.5 x 5%CT 08003761 8 Tomato, paste
- babyfood 0.164000 7.100 0.075 1.5 x Full comment: 1.5 x 5%CT 08003770 8 Tomato, puree 0.164000 2.900 0 .075 1.5 x Full comment: 1.5 x 5%CT 08003771 8 Tomato, puree
- babyfood 0.164000 2.900 0.075 1.5 x Full comment: 1.5 x 5%CT 08003780 8 Tomato, dried 0.164000 14.300 0.075 1.5 x Full co mment: 1.5 x 5%CT 08003781 8 Tomato, dried
- babyfood 0.164000 14.300 0.075 1.5 x Full comment: 1.5 x 5%CT 08003790 8 Tomato, juice 1.000000 1.000 1.500 11 09010750 9A Cantaloupe 1.000000 1.000 1.500 20 09010800 9A Casaba 1.000000 1.000 1.500 20 09011870 9A Honeydew melon 1.000000 1.000 1.500 20 09013990 9A Watermelon 1.000000 1.00 0 1.500 20 09014000 9A Watermelon, juice 1.000000 1.000 1.500 20 09020210 9B Balsam pear 1.000000 1.000 1.500 21 09020880 9B Chayote, fruit 1.000000 1.000 1.500 22 0902 1020 9B Chinese waxgourd 1.000000 1.000 1.500 21 09021350 9B Cucumber 1.000000 1.000 1.500 22 09023080 9B Pumpkin 1.000000 1.000 1.500 22 09023090 9B Pumpkin, seed 1.000000 1.000 1.500 22 09023560 9B Squash, summer 1.000000 1.000 1.500 22 09023561 9B Squash, summer
- babyfood 1.000000 1.000 1.500 22 09023570 9B Squash, winter 1.000000 1.000 1.500 22 09023571 9B Squash, winter
- babyfood 1.000000 1.000 1.500 22 13020570 13B Blueberry 0.150000 1.000 1.500 24 13020571 13B Blueberry
- babyfood 0.150000 1 .000 1.500 24 86010000 O Water, direct, all sources 0.017000 1.000 1.000 26 86020000 O Water, indirect, all sources 0.017000 1.000 1.000 26 95000230 O Banana 0.100000 1.000 1.500 1 9 5000231 O Banana
- babyfood 0.100000 1.000 1.500 1 95000240 O Banana, dried 0.010700 3.900 1.500 95000241 O Banana, dried
- babyfood 0.010700 3.900 1.500 95001300 O Cranbe rry 0.150000 1.000 1.500 24 95001301 O Cranberry
- babyfood 0.150000 1.000 1.500 24 95001310 O Cranberry, dried 0.150000 1.000 1.500 24 95001320 O Cranberry, juice 0.150000 1.000 1.500 24

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 16 of 26 95001321 O Cranberry, juice
- babyfood 0.150000 1.000 1.500 24 95002830 O Plantain 0.100000 1.000 1.500 1 95002840 O Plantain, dried 0.010700 3.900 1.500 95003590 O Strawberry 0.150000 1.000 1.500 24 95003591 O Strawberry
- babyfood 0.150000 1.000 1.500 24 95003600 O Strawberry, juice 0.150000 1.000 1.500 24 95003601 O Strawberry, juice
- babyfood 0.150000 1.000 1.500 24

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 17 of 26 Attachment 2 Results of Acute DEEM
- FCI D Dietary Exposure Analy sis U.S. Environmental Protection Agency Ver. 2.02 DEEM - FCID ACUTE Analysis f or ACIBENZOLAR - S - METHYL (1994
- 98 data) Residue file: 061402AcuteConvertedMetaboliteWater.R98 Adjustment factor #2 used. Analysis Date: 07
- 19
- 2011/10:13:04 Residue file dated: 06
- 28
- 2011/07:37:37/8 Daily totals for food and foodform co nsumption used. MC iterations = 1000 MC list in residue file MC seed = 10281 Run Comment: ""

=============================================================================== Summary calculations (per capita): 95th Percentile 99th Percentile 99.9th Percentile Exposure % aRfD Exposure % aRfD Exposure % aRfD

---------- -------- ---------- -------- ---------- -------- U.S. Population: 0.001353 1.6 5 0.004014 4.90 0.014120 17.22 All infants: 0.002414 2.94 0.005284 6.44 0.013034 15.89 Children 1
- 2 yrs: 0.002618 3.19 0.008470 10.33 0.025051 30.55 Children 3
- 5 yrs: 0.002464 3.00 0.009451 11.53 0.028600 34.88 Children 6
- 12 yrs: 0.001409 1.72 0.005754 7.02 0.019931 24.31 Youth 13
- 19 yrs: 0.000985 1.20 0.003739 4.56 0.015067 18.37 Adults 20
- 49 yrs: 0.001095 1.33 0.003041 3.71 0.009085 11.08 Adults 50+ yrs: 0.001537 1.87 0.003851 4.70 0.010651 12.99 Females 13
- 49 yrs: 0.001169 1.43 0.003208 3.91 0.009561 11.66

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 18 of 26 Attachment 3 Chronic DEEM
- FCI D Residue Input File. Filename: C: \ Deem Files \ 061402 Acibenzolar \ Acibenzolar on Low Growing Berries \ 061402Chronic.R98 Chemical: Acibenzolar
- S - Methyl RfD(Chronic ): .082 mg/kg bw/day NOEL(Chronic): 0 mg/kg bw/day RfD(Acute): .082 mg/kg bw/day NOEL(Acute): 0 mg/kg bw/day Date created/last modified: 06
- 22
- 2011/09:35:41/8 Program ver. 2.03

------------------------------------------------------------------- ------------- EPA Crop Def Res Adj.Factors Comment Code Grp Commodity Name (ppm) #1 #2

-------- ---- ------------------------------- ---------- ------ ------ ------- 03002 370 3 Onion, dry bulb 0.100000 1.000 1.500 03002371 3 Onion, dry bulb
- babyfood 0.100000 1.000 1.500 03002380 3 Onion, dry bulb, dried 0.100000 9.000 1.500 03002381 3 Onion, dry bulb, dried
- babyfood 0.100000 9.000 1.500 03002390 3 Onion, green 0.100000 1.000 1.500 03003380 3 Shallot 0.100000 1.000 1.500 04010050 4A Amaranth, leafy 0.250000 1.000 1.500 04010180 4A Arugula 0.250000 1.000 1.500 04011040 4A Chrysanthemum, garland 0.250000 1.000 1.500 04011330 4A Cress, garden 0.250000 1.000 1.500 04011340 4A Cress, up land 0.250000 1.000 1.500 04011380 4A Dandelion, leaves 0.250000 1.000 1.500 04011500 4A Endive 0.250000 1.000 1.500 04012040 4A Lettuce, head 0.250 000 1.000 1.500 04012050 4A Lettuce, leaf 0.250000 1.000 1.500 04012480 4A Parsley, leaves 0.250000 1.000 1.500 04013130 4A Radicchio 0.250000 1.000 1.500 04013550 4 A Spinach 1.000000 1.000 1.500 04013551 4A Spinach
- babyfood 1.000000 1.000 1.500 04020760 4B Cardoon 0.250000 1.000 1.500 04020850 4B Celery 0.250000 1.000 1.500 04020851 4B Celery
- babyfood 0.250000 1.000 1.500 04020860 4B Celery, juice 0.250000 1.000 1.500 04020870 4B Celtuce 0.250000 1.000 1.500 04021520 4B Fennel, Florence 0.250000 1.000 1.500 04023220 4B Rhubarb 0.250000 1.000 1.500 04023670 4B Swiss chard 0.250000 1.000 1.500 05010610 5A Broccoli 1.000000 1.000 1.500 05010611 5A Broccoli
- babyfood 1.000000 1.000 1.500 05010620 5A Broccoli, Chinese 1.000000 1.000 1.500 05010640 5A Brussels sprouts 1.000000 1.000 1.500 05010690 5A Cabbage 1.000000 1.000 1.500 05010710 5A Cabbage, Chinese, napa 1.000000 1.000 1.500 05010720 5A Cabbage, Chinese, mustard 1.000000 1.000 1.500 05010830 5A C auliflower 1.000000 1.000 1.500 05011960 5A Kohlrabi 1.000000 1.000 1.500 05020630 5B Broccoli raab 1.000000 1.000 1.500 05020700 5B Cabbage, Chinese, bok choy 1.000000 1.000 1.500 05021170 5B Collards 1.000000 1.000 1.500 05021940 5B Kale 1.000000 1.000 1.500 05022290 5B Mustard greens 1.000000 1.000 1.500 05 023180 5B Rape greens 1.000000 1.000 1.500 05023890 5B Turnip, greens 1.000000 1.000 1.500 08001480 8 Eggplant 1.000000 1.000 1.500 08002340 8 Okra 1.000000 1.000 1.500 08002700 8 Pepper, bell 1.000000 1.000 1.500 08002701 8 Pepper, bell
- babyfood 1.000000 1.000 1.500 08002710 8 Pepper, bell, dried 1.000000 1.00 0 1.500 08002711 8 Pepper, bell, dried
- babyfood 1.000000 1.000 1.500 08002720 8 Pepper, nonbell 1.000000 1.000 1.500 08002721 8 Pepper, nonbell
- babyfood 1.000000 1.000 1.500 08002730 8 Pepper , nonbell, dried 1.000000 1.000 1.500 08003740 8 Tomatillo 1.000000 1.000 1.500 08003750 8 Tomato 1.000000 1.000 1.500 08003751 8 Tomato
- babyfood 1. 000000 1.000 1.500 08003760 8 Tomato, paste 3.000000 1.000 1.500 08003761 8 Tomato, paste
- babyfood 3.000000 1.000 1.500 08003770 8 Tomato, puree 1.000000 2.900 1.500 0800377 1 8 Tomato, puree
- babyfood 1.000000 2.900 1.500

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 19 of 26 08003780 8 Tomato, dried 1.000000 14.300 1.500 08003781 8 Tomato, dried
- babyfood 1.000000 14.300 1.500 08003790 8 Tomato, juice 1.000000 1.000 1.500 09010750 9A Cantaloupe 2.000000 1.000 1.500 09010800 9A Casaba 2.000000 1.000 1.500 09011870 9A Honeydew melon 2.000000 1.000 1. 500 09013990 9A Watermelon 2.000000 1.000 1.500 09014000 9A Watermelon, juice 2.000000 1.000 1.500 09020210 9B Balsam pear 2.000000 1.000 1.500 09020880 9B Chayote, fr uit 2.000000 1.000 1.500 09021020 9B Chinese waxgourd 2.000000 1.000 1.500 09021350 9B Cucumber 2.000000 1.000 1.500 09023080 9B Pumpkin 2.00000 0 1.000 1.500 09023090 9B Pumpkin, seed 2.000000 1.000 1.500 09023560 9B Squash, summer 2.000000 1.000 1.500 09023561 9B Squash, summer
- babyfood 2.000000 1.000 1.500 09023570 9B Squash, winter 2.000000 1.000 1.500 09023571 9B Squash, winter
- babyfood 2.000000 1.000 1.500 13020570 13B Blueberry 0.150000 1.000 1.500 13020571 13B Blueberry
- babyfood 0.150000 1.000 1.500 86010000 O Water, direct, all sources 0.009460 1.000 1.000 86020000 O Water, indirect, all sources 0.009460 1.000 1.000 95000230 O Banana 0.100000 1.000 1.500 95000231 O Banana
- babyfood 0.100000 1.000 1.500 95000240 O Banana, dried 0.100000 3.900 1.500 95000241 O Banana, dried
- babyfood 0.100000 3.900 1.500 95001300 O Cranberry 0.150000 1.000 1.500 95001301 O Cranberry
- babyfood 0.150000 1.000 1.500 95001310 O Cranberry, dried 0.150000 1.000 1.500 95001320 O Cranberry, juice 0.150000 1 .000 1.500 95001321 O Cranberry, juice
- babyfood 0.150000 1.000 1.500 95002830 O Plantain 0.100000 1.000 1.500 95002840 O Plantain, dried 0.100000 3.900 1.500 95003590 O Str awberry 0.150000 1.000 1.500 95003591 O Strawberry
- babyfood 0.150000 1.000 1.500 95003600 O Strawberry, juice 0.150000 1.000 1.500 95003601 O Strawberry, juice
- babyfood 0.150000 1.000 1.500

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 20 of 26 Attachment 4 Results of Chronic DEEM
- FCI D Dietary Exposure Analy sis. U.S. Environmental Protection Agency Ver. 2.00 DEEM - FCID Chronic analysis for ACIBENZOLAR
- S - METHYL (1994
- 98 data ) Residue file name: C: \ Documents and Settings \ tmorton \ My Documents \ MyFiles \ DEEM Files \ 061402 Acibenzolar \ Acibenzolar on Low Growing Berries \ 061402Chronic.R98 Adjustment factor #2 used. Analysis Date 07
- 19
- 2011/11:14:22 Residue file dated: 06
- 24
- 2011/15:53:14/8 Reference dose (RfD, Chronic) = .082 mg/kg bw/day

=============================================================================== Total exposure by population subgroup

----- -------------------------------------------------------------------------- Total Exposure

----------------------------------- Population mg/kg Percent of Subgroup body wt/day PAD

-------------------------------------- ------------- --------------- U.S. Population (total) 0.004366 5.3% U.S. Population (spring season) 0.004431 5.4% U.S. Population (summer season) 0.005515 6.7% U.S. Population (autumn season) 0.003818 4.7% U.S. Population (win ter season) 0.003679 4.5% Northeast region 0.004574 5.6% Midwest region 0.004261 5.2% Southern region 0.004000 4.9% Western region 0.004871 5.9% Hispanics 0.004983 6.1% Non - hispanic whites 0.004220 5.1% Non - hispanic blacks 0.004024 4.9% Non - hisp/non
- white/non
- black 0.006247 7.6% All infants (< 1 year) (.082*) 0.003808 4.6% Nursing infants (.082*) 0 .002360 2.9% Non - nursing infants (.082*) 0.004358 5.3% Children 1
- 6 yrs (.082*) 0.008690 10.6% Children 7
- 12 yrs (.082*) 0.005490 6.7% Fema les 13
- 19 (not preg or nursing) (.082*) 0.003654 4.5% Females 20+ (not preg or nursing) (.082*) 0.003757 4.6% Females 13
- 50 yrs (.082*) 0.003897 4.8% Females 13+ (preg/not nursing) (.082*) 0.003521 4.3% Females 13+ (nursing) (.082*) 0.004935 6.0% Males 13
- 19 yrs (.25*) 0.004129 1.7% Males 20+ yrs (.25*) 0.003680 1.5% Seniors 55+ (.25*) 0.003886 1.6% Children 1
- 2 yrs (.082*) 0.009207 11.2% Children 3
- 5 yrs (.082*) 0.008706 10.6% Children 6
- 12 yrs (.082*) 0.005812 7.1% Youth 13
- 19 yrs (.082*) 0.003893 4.7% Adults 20
- 49 yrs (.25*) 0.003654 1.5% Adults 50+ yrs (.25* ) 0.003852 1.5% Females 13
- 49 yrs (.082*) 0.003646 4.4% *PAD in mg/kg
- bw - day

-------------------------------------------------------------------------------

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 21 of 26 **Attachment 5 Perc ent Crop Treated Estimates Provided by BEAD** 

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 22 of 26

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 23 of 26

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 24 of 26 6. RDF Files Used in Acute Analy sis

RDF #1 Banana %CT = 100 TOTALNZ = 30 TOTALZ = 0 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.02 0.04 0.03 0.04 0.07 0.02 0.04 0.05 RDF #2 Bell Pepper % CT= 10 TOTALNZ=9 TOTALZ= 8 1 0.16 0.28 0.34 0.40 0.53 0.29 0.27 0.30 0.32 RDF #3 Broccoli % CT= 5 TOTALNZ=11 TOTALZ=209 0.58 0.28 0.34 0.42 0.52 0.17 0.22 0.32 0.60 0.60 0.63 RDF #4 Cabbage % CT= 1 TOTALNZ=11 TOTALZ= 1089 0.57 0.19 0.22 0.12 0.14 0.28 0.36 0.06 0.09 0.27 0.35 RDF #5 Celery % CT= 100 TOTALNZ=9 TOTALZ= 0 0.045 0.018 0.016 0.050 0.043 0.058 0.042 0.07 7 0.063

RDF #6 Head Lettuce % CT= 15 TOTALNZ=12 TOTALZ= 6 8 0.081 0.075 0.049 0.074 0.033 0.041 0.044 0.039 0.105 0.093 0.043 0.055 RDF #7 Hot Pepper % CT= 10 TOTALNZ=5 TOTALZ= 4 5 0.50 0.41 0.35 0.21 0.32 RDF #8 Leaf Lettuce % CT= 15 TOTALNZ=11 T OTALZ= 62 0.099 0.151 0.136 0.073 0.035 0.112 0.183 0.185 0.175 0.037 0.044 RDF #9 Mustard Greens % CT= 100 TOTALNZ=9 TOTALZ=0 0.74 0.53 0.64 0.71 0.80 0.26 0.32 0.14 0.18 RDF #10 Spinach % CT= 4 0 TOTALNZ=11 TOTALZ= 17 0.273 0.326 0.247 0.418 0.538 0.110 0 .138 0.232 0.262 0.230 0.190

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 25 of 26 RDF #11 Tomato % CT= 5 TOTALNZ=23 TOTALZ=437 0.13 0.21 0.21 0.06 0.08 0.46 0.25 0.11 0.16 0.09 0.09 0.09 0.09 0.06 0.08 0.14 0.08 0.16 0.20 0.22 0.32 0.22 0.27 RDF #12 BellPepperOth % CT= 100 TOTALNZ=9 TOTALZ=0 0.16 0.28 0.34 0.40 0.53 0.29 0.27 0.30 0.32 RDF #13 BroccoliOth % CT= 100 TOTALNZ=11 TOTALZ=0 0.58 0.28 0.34 0.42 0.52 0.17 0.22 0.32 0.60 0.60 0.63 RDF #14 CabbageOth % CT= 100 TOTALNZ=11 TOTALZ=0 0.57 0.19 0.22 0.12 0.14 0.28 0.36 0.06 0.09 0.27 0.35 RDF #15 CeleryOth % CT= 100 TOTALNZ=9 TOTALZ=0 0.045 0.018 0.016 0.050 0.043 0.058 0.042 0.077 0.063

RDF #16 LettuceHRad % CT= 100 TOTALNZ=12 TOTALZ=0 0.081 0.075 0.049 0.074 0.033 0.041 0.044 0.039 0.105 0.093 0.043 0.055 RDF #17 HotPepperOth % CT= 100 TOTALNZ=5 TOTALZ=0 0.50 0.41 0.35 0.21 0.32 RDF #18 SpinachOth % CT= 100 TOTALNZ=11 TOTALZ=0 0.273 0.326 0.247 0.418 0.538 0.110 0.138 0.232 0.262 0.230 0.190 RDF #19 TomatoOth % CT= 100 TOTALNZ= 23 TOTALZ=0 0.13 0.21 0.21 0.06 0.08 0.46 0.25 0.11 0.16 0.09 0.09 0.09 0.09 0.06 0.08 0.14 0.08 0.16 0.20 0.22 0.32 0.22 0.27 RDF #20 Cantaloupe % CT= 100 TOTALNZ=6 TOTALZ=0 0.235 0.759 0.240 0.279 0.278 0.134

### Acibenzolar
- S
- Methyl (PC C ode 061402 ) Dietary Exposure and Risk Assessment DP #: 387069



## Page 26 of 26 RDF #21 Cucumber % CT= 100 TOTALNZ=8 TOTALZ=0 0.166 0.508 0.183 0.210 0.218 0.215 0.155 0.132 RDF #22 Squash % CT= 100 TOTALNZ=5 TOTALZ=0 0.152 0.418 0.151 0.187 0.172 RDF #23 Onion % CT= 100 TOTALNZ=7 TOTALZ=0 0.025 0.025 0.025 0.056 0.025 0.025 0.025 RDF #24 Strawberry %CT=100 TOTALNZ= 20 TOTALZ=0 0.020 0.032 0.035 0.023 0.050 0.040 0.067 0.058 0.064 0.062 0.040 0.038 0.076 0.054 0.083 0.087 0.020 0.022 0.023 0.025 RDF # 25 Cauliflower % CT= 10 TOTALNZ=11 TOTALZ= 99 0.58 0.28 0.34 0.42 0.52 0.17 0.22 0.32 0.60 0.60 0.63