# Patent

## Title
Method of predicting risk of lung cancer recurrence, and a composition, kit and microarray for the same

## Abstract

Provided is a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method including: obtaining a biological sample from a lung cancer patient; measuring an expression level of at least one marker gene from the biological sample, the marker gene being selected from the group consisting of marker genes of Table 1, 2 or 3, to obtain data for the expression level of the marker gene; and determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group.

## Background
CROSS-REFERENCE TO RELATED PATENT APPLICATION
This application claims the benefit of Korean Patent Application No. 10-2007-0002643, filed on Jan. 9, 2007, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein in its entirety by reference.
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, a method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, a report prepared by the same, and a composition, kit and microarray for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
2. Description of the Related Art
Lung cancer is the leading cause of death due to cancer in the world. Lung cancer is categorized into two types, small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), and about 80% of lung cancer cases are categorized as NSCLC. NSCLC is categorized into three sub-types: 40% of adenocarcinoma, 40% of squamous cell carcinoma and 20% of large cell carcinoma. Currently, a TMN staging system is widely accepted in the management of lung cancer.
In the TMN staging system, the primary tumor is subdivided into four T categories (T1-T4) depending upon the tumor size, site and local involvement. Lymph node spread is subcategorized into bronchio/pulmonary within the lung (N1), mediastinal spread on the same side of the lung as the primary tumor (N2) and mediastinal spread on the side of the lung opposite to the side having the primary tumor or supraclavicular involvement (N3). Distal or metastatic spread is either absent or present (M0 or M1). In general, lung cancer that does not metastasize is treated by being removed through a surgical operation. However, recurrence rate after a lung cancer removal operation is as high as 20 to 50% (Cancer: Principles & Practice of Oncology, 56th. ed. In: Devita D V, Hellman S. Rosenberg S A, eds. Philadelphia, Pa.: Lippincott Williams & Wilkins, 2001).
Conventionally, a method of diagnosing lung cancer using a marker gene specific to lung cancer is known. For example, U.S. Patent Publication No. 2006025057 discloses a method of diagnosing lung cancer using a marker specific to lung cancer. Further, U.S. Patent Publication No. 20050272061 discloses a method of diagnosing cancer in an individual, comprising measuring an L gene that is specifically and distinctively expressed in lung cancer tissues and cells, and its products.
However, there is still a need for developing a method of effectively predicting the risk of lung cancer recurrence in a lung cancer patient or a patient who has had lung cancer treatment to the extent that the method is applied to clinical practices.
SUMMARY OF THE INVENTION
The present invention provides a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
The present invention also provides a method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment and a report prepared by the same.
The present invention also provides a composition, kit and microarray for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
According to an aspect of the present invention, there is provided a method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method comprising:
obtaining a biological sample from a lung cancer patient;
measuring an expression level of at least one marker gene from the biological sample, the marker gene being selected from the group consisting of marker genes of Table 1, 2 or 3 to obtain data for the expression level of the marker gene; and
determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group.
The method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment includes obtaining a biological sample from a lung cancer patient.
The obtaining a biological sample may include any operation that obtains a sample including an arbitrary cell from a lung cancer patient. For example, the biological sample may be blood, plasma, serum, urine, tissue, cell, organ, bone marrow, saliva, expectoration, cerebrospinal fluid and the like, but is not limited thereto. The biological sample may be preferably lung cancer tissue. The biological sample may be lung cancer tissue removed during a lung cancer removal operation, but is not necessarily obtained by the lung cancer removal operation. The obtainment of the lung cancer tissue may be physically conducted or optically conducted through a laser or the like.
The method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment includes measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 1, 2 or 3 in the sample to obtain data for the expression level of the marker gene.
The measuring an expression level of the marker gene may be performed by measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 1. Preferably, in this operation, expression levels of at least 2, 4, 6, 8, 10, 15, 20, 30, 70, 100, 150 or a total of 166 marker genes selected from the group consisting of marker genes of Table 1 may be measured. In this case, the lung cancer may be adenocarcinoma or squamous cell carcinoma.
When the lung cancer is adenocarcinoma, the measuring an expression level of the marker gene may be performed by measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 2. Preferably, in this operation, expression levels of at least 2, 4, 6, 8, 10, 15, 20, 30, 70, 100, 150, 200, 250 or a total of 300 marker genes selected from the group consisting of marker genes of Table 2 may be measured.
When the lung cancer is squamous cell carcinoma, the measuring an expression level of the marker gene may be performed by measuring an expression level of at least one marker gene selected from the group consisting of marker genes of Table 3. Preferably, in this operation, an expression level of at least 2, 4, 6, 8, 10, 15, 20, 30, 70, 100, 150, or a total of 166 marker genes selected from the group consisting of marker genes of Table 3 may be measured.
The measuring an expression level of the marker gene includes measuring an arbitrary expression product expressed from the maker gene. For example, this operation may be measuring a level of mRNA or protein derived from the marker gene.
The “measurement of a level of mRNA” may be analyzed using a conventional method including RT-PCR, competitive RT-PCR, real-time RT-PCR, RNase protection assay, northern blotting, DNA microarray and the like. Preferably, the measurement of a level of mRNA may be carried out by hybridizing mRNA isolated from the biological sample or cDNA derived therefrom on a microarray on which a probe specific to at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3 is immobilized to measure a degree of the obtained hybridization. The degree of the hybridization may be measured using an arbitrary measurement method known to those of ordinary skill in the art, such as fluorescence measurement and electrical measurement. In this case, the probe or target nucleic acid may be labeled with a detectable appropriate marker. Herein, the cDNA may be directly amplified by RT-PCR using sense and anti-sense primer pair targeted to at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3 as a primer.
The “measurement of a level of protein” may be conducted using any conventional protein measuring or detecting method. For example, the measurement of a level of protein may be conducted using an analysis method that uses an antibody that specifically binds with protein expressed from at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3. Examples of the protein analysis method using an antibody may include western blotting, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, radioimmunodiffusion, Ouchterlony immunodiffusion, rocket immunoelectrophoresis, immunoprecipitation assay, complement fixation analysis, Fluorescence Activated Cell Sorting (FACS) and the like, but are not limited thereto. Examples of the ELISA include a direct ELISA, an indirect ELISA, a direct sandwich ELISA, an indirect sandwich ELISA and the like. The western blotting is a method in which total protein is isolated and electrophoresized to separate protein according to their size, the separated proteins are then moved into a nitrocellulose membrane to be reacted with an antibody, and a generated amount of the antigen-antibody complex is confirmed using a labeled antibody. In addition, the level of protein may be measured using enzyme, substrate, coenzyme, ligand or the like that specifically binds with the target protein.
The expression level of the marker gene may be determined by measuring an amount of an amplification product obtained by nucleic acid amplification that is carried out by a reverse transcriptase-polymerase chain reaction (RT-PCR) using RNA isolated from the sample as a template.
In addition, the method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment includes determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group.
The term “recurrence group” refers to a group of patients with lung cancer recurrence within a certain period after a lung cancer treatment among lung cancer patients. Preferably, the term “recurrence group” may refer to a group of patients with lung cancer recurrence within one year after a lung cancer removal operation among lung cancer patients. However, types of lung cancer treatment and a period which is a basis of recurrence may be appropriately adjusted by those of ordinary skill in the art. In addition, the term “non-recurrence group” refers to a group of patients without lung cancer recurrence even after a certain period passes by after a lung cancer treatment among lung cancer patients. Preferably, the term “non-recurrence group” refers to a group of patients without lung cancer recurrence even after three years after a lung cancer removal operation among lung cancer patients. However, types of lung cancer treatment and a period which is a basis of non-recurrence may be appropriately adjusted by those of ordinary skill in the art.
The “expression level of recurrence group” or “expression level of non-recurrence group” corresponds to a standard expression level. Through preliminary experiment, a biological sample of a lung cancer patient, for example, lung cancer tissue is collected in advance. An expression level of at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3 in the lung cancer tissue is then measured. Patients after lung cancer treatment are divided into a recurrence group and a non-recurrence group in which recurrence and non-recurrence respectively occur as time passes by. Next, each of expression levels of the marker gene measured in the recurrence and non-recurrence groups is divided into an expression level of the recurrence group or the non-recurrence group.
The determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group may be performed using a statistical forecasting model. In this case, whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group is determined by whether the expression levels show a statistically meaningful difference from each other.
Whether there is a statistically meaningful difference may be determined using a statistical analysis model known to those of ordinary skill in the art. Preferably, the statistical analysis model may be a statistical forecasting model selected from the group consisting of a Linear Discrimination Analysis (LDA) model, a Quadratic Discrimination Analysis (QDA) prediction model, a Neural Network model, a Decision Tree model, a Support Vector Machine model and a Naive Bayes model, but is not limited thereto.
Examples of the determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group include determining to correspond to a non-recurrence group if the expression level of the marker gene shows a statistically meaningful difference from the expression level of the recurrence group, and determining to correspond to a recurrence group if the expression level of the marker gene shows a statistically meaningful difference from the expression level of the non-recurrence group. In addition, examples of the determining whether the expression level of the marker gene corresponds to an expression level of a recurrence group or an expression level of a non-recurrence group include determining to correspond to a recurrence group if the expression level of the marker gene does not show a statistically meaningful difference from the expression level of the recurrence group, and determining to correspond to a non-recurrence group if the expression level of the marker gene does not show a statistically meaningful difference from the expression level of the non-recurrence group.
The statistically meaningful difference may have p values that are statistically meaningfully higher or lower than the expression level of the recurrence group or non-recurrence group. Preferably, the p value may be less than 0.05.
In the method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, if the expression level of the marker gene is determined to correspond to the expression level of the recurrence group, a risk of lung cancer recurrence in a patient can be predicted to be high. In addition, if the expression level of the marker gene is determined to correspond to the expression level of the non-recurrence group, a risk of lung cancer recurrence in a patient can be predicted to be low.
In the method of predicting a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, specificity may be at least 50%, preferably 60%, more preferably at least 70%, far more preferably at least 80%, and most preferably 90%.
According to another aspect of the present invention, there is provided a method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method comprising preparing a report representing predicted results according to the method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
The report may include probability of recurrence according to time.
According to another aspect of the present invention, there is provided a report on a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, which is prepared by the method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment.
According to another aspect of the present invention, there is provided a composition for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising at least one probe or probe set selected from marker genes selected from the group consisting of marker genes of Tables 1, 2 and 3.
The composition may further comprise a reagent required for hybridization reaction with the marker gene in a sample or nucleic acid products expressed therefrom. In addition, the composition may further comprise a buffer, a solvent or the like that stabilizes the probe and acts as a medium of the reaction.
The term “probe” used through the present application refers to a nucleic acid strand that is partially or completely complementary to a target nucleic acid, and refers to oligonucleotide that can bind with the target nucleic acid by a base-specific method. Preferably, the probe may be oligonucleotide that is completely complementary to the target nucleic acid. The probe can be a conventionally known arbitrary nucleic acid derivative that can complementarily bind to the target nucleic acid, such as peptide nucleic acid as well as nucleic acid.
The binding of the probe with the target nucleic acid (in general, referred to as hybridization) may be sequence-dependently carried out under various conditions. In general, the hybridization is performed in a specific ion intensity at specific pH at a temperature that is about 5° C. lower than Tm with respect to a specific sequence. The Tm refers to a state at which 50% of probe complementary to a target sequence is bound to the target sequence. Examples of the conditions of the hybridization may include a pH in the range of 7.0-8.3 and a Na+ ion concentration of 0.01-1.0 M. In addition, to raise specificities of the target nucleic acid and the probe, the hybridization may be carried out under conditions that make the binding of the probe with the target nucleic acid unstable, for example, at a high temperature and in the presence of a high concentration of an unstabilizing agent (for example formamide).
The probe may be any length of polynucleotide that can sequence-specifically be bound to the target nucleic acid. For example, the length of the probe may be 7-200 nucleotides, 7-150 nucleotides, 7-100 nucleotides, 7-50 nucleotides, or a full-length strand of gene, but is not limited thereto.
The probe may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
According to another aspect of the present invention, there is provided a kit for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising at least one probe or probe set selected from marker genes selected from the group consisting of marker genes of Tables 1, 2 and 3.
The probe is the same as defined above. The probe may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
In the kit, the probe or probe set may be immobilized on a microarray. A target nucleic acid in a sample is hybridized with the probe on the microarray, and the presence and concentration of the target nucleic acid may be determined by measuring the hybridized results. During the hybridization, the target nucleic acid may be labeled with a detectable marker.
The kit may further include a manual that describes a process of measuring a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment.
According to another aspect of the present invention, there is provided a kit for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising sense and anti-sense primer pair with respect to at least one marker gene selected from the group consisting of marker genes of Tables 1, 2 and 3.
The term “primer” used herein refers to a nucleic acid having a free 3′ hydroxy group that is partially or completely complementary to a target nucleic acid and can bind with the template nucleic acid by a sequence-specific method, and refers to oligonucleotide that functions as a starting point for template strand transcription in polymerization.
The kit may further comprise a reagent required for PCR or RT-PCR using the primer described above as a primer and the target nucleic acid as a template. The reagent may include a buffer solution, a DNA polymerase (and/or reverse transcriptase), and 4 types of dNTPs.
The primer may be any length of polynucleotide that can sequence-specifically be bound to the template nucleic acid and function as a starting point for template strand transcription in polymerization. For example, the length of the primer may be 7-200 nucleotides, 7-150 nucleotides, 7-100 nucleotides, 7-50 nucleotides, or a full-length strand of a gene, but is not limited thereto.
The primer may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
According to another aspect of the present invention, there is provided a microarray for diagnosing a risk of lung cancer recurrence in a lung cancer patient or a patient after a lung cancer treatment, in which at least one probe or probe set selected from marker genes selected from the group consisting of marker genes of Tables 1, 2 and 3.
The term “microarray” refers to a polynucleotide group immobilized on a substrate in a high concentration. The polynucleotide group is respectively immobilized on a certain region. Such microarray is well-known to those of ordinary skill in the art. The microarray is, for example, disclosed in U.S. Pat. Nos. 5,445,934 and 5,744,305, and contents of these patents are included in the present application by reference. The substrate may have various shapes such as plate, film and microsphere (or bead).
The probe is the same as defined above. The probe may be labeled with a detectable marker. The detectable marker may be a fluorescent marker such as Cy3 or Cy5, a radioactive material marker, enzyme that converts a substrate to chromogen, or the like, but is not limited thereto.
The gene expression pattern of the lung cancer cell after lung cancer tissue removal operation is analyzed through a hybridization with the probe on the microarray, and a marker gene that is determined to have a difference in an expression level between a patient with lung cancer recurrence within one year (recurrence group) and a patient without lung cancer recurrence even after three years (non-recurrence group) is selected. The results are shown in Table 1 below. A total number of patients was 60. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 19, and the number of patients without lung cancer recurrent even after three years was 41.













TABLE 1









Genbank
T-test
Fold change


NO.
Probe Set ID
Gene Name
Gene Symbol
Accession #
p-value
(abs)





















001
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.005162234
1.522293


002
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.019467462
2.3323212


003
1553530_a_at
integrin, beta 1 (fibronectin receptor, beta polypeptide,
ITGB1
NM_033669
0.01684671
1.7791877




antigen CD29 includes MDF2 MSK12)


004
1553678_a_at
integrin, beta 1 (fibronectin receptor, beta polypeptide,
ITGB1
NM_133376
0.012459265
1.7374801




antigen CD29 includes MDF2, MSK12)


005
1554087_at
hypothetical protein FLJ32549
FLJ32549
BC036246
0.002290308
1.5143739


006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397
BC010850
0.001210456
1.6267678


007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin gamma)
ADAM9
AF495383
0.012324799
2.1980886


008
1555564_a_at
I factor (complement)
IF
BC020718
0.007528743
2.5875902


009
1555705_a_at
chemokine-like factor superfamily 3
CKLFSF3
AY168714
0.004961676
1.8587251


010
1557987_at
PI-3-kinase-related kinase SMG-1-like locus
LOC641298
BC042832
0.010989661
1.7944587


011
1558678_s_at
metastasis associated lung adenocarcinoma transcript 1
MALAT1
BE708432
0.00670648
1.6990829




(non-coding RNA)


012
160020_at
matrix metallopeptidase 14 (membrane-inserted)
MMP14
Z48481
0.005463324
1.5193439


013
200604_s_at
protein kinase, cAMP-dependent, regulatory,
PRKAR1A
M18468
0.017312625
1.5803499




type I, alpha (tissue specific extinguisher 1)


014
200615_s_at
adaptor-related protein complex 2, beta 1 subunit
AP2B1
AL567295
0.007407852
1.6839108


015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.000163535
1.5653288


016
200922_at
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum
KDELR1
NM_006801
0.004791257
1.638207




protein retention receptor 1


017
201020_at
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase
YWHAH
NM_003405
0.009279575
1.5148095




activation protein, eta polypeptide


018
201179_s_at
guanine nucleotide binding protein (G protein),
GNAI3
J03005
0.014834337
1.5069977




alpha inhibiting activity polypeptide 3


019
201309_x_at
chromosome 5 open reading frame 13
C5orf13
U36189
0.011555359
2.1326842


020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.00119686
1.5838884


021
201505_at
laminin, beta 1
LAMB1
NM_002291
0.000568398
1.8073287


022
201506_at
transforming growth factor, beta-induced, 68 kDa
TGFBI
NM_000358
0.008768089
1.9059453


023
201548_s_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
W02593
0.010550437
1.5276276


024
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.002245945
2.1570368


025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.007795681
2.1724482




(Strongylocentrotus purpuratus)


026
201578_at
podocalyxin-like
PODXL
NM_005397
0.00303411
1.8943018


027
201617_x_at
caldesmon 1
CALD1
NM_004342
0.01926877
1.8294148


028
201646_at
scavenger receptor class B, member 2
SCARB2
AA885297
0.006063032
1.6768507


029
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.015885489
1.6841809


030
201695_s_at
nucleoside phosphorylase
NP
NM_000270
0.018524641
1.6833633


031
201722_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT1
AV692127
0.009770202
1.5369248




N-acetylgalactosaminyltransferase 1 (GalNAc-T1)


032
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.00259865
1.6228764


033
201942_s_at
carboxypeptidase D
CPD
D85390
0.017363481
1.7431495




(melanoma growth stimulating activity, alpha)


034
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.004330024
2.8191426


035
202543_s_at
glia maturation factor, beta
GMFB
BC005359
0.008048828
1.5254242


036
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.002003783
1.767903


037
202627_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
AL574210
0.00091248
3.0523725




plasminogen activator inhibitor type 1), member 1


038
202628_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
NM_000602
0.00504642
2.6835847




plasminogen activator inhibitor type 1), member 1


039
202817_s_at
synovial sarcoma translocation, chromosome 18
SS18
NM_005637
0.005462693
1.5148987


040
202859_x_at
interleukin 8
IL8
NM_000584
0.014948112
2.1844351


041
202936_s_at
SRY (sex determining region Y)-box 9
SOX9
NM_000346
0.019816045
2.2876046




(campomelic dysplasia, autosomal sex-reversal)


042
202949_s_at
four and a half LIM domains 2
FHL2
NM_001450
0.006776552
2.2249734


043
202998_s_at
lysyl oxidase-like 2
LOXL2
NM_002318
0.006687925
2.0231075


044
203066_at
B cell RAG associated protein
GALNAC4S-6ST
NM_014863
0.00419499
1.5032523


045
203072_at
myosin IE
MYO1E
NM_004998
0.000449373
1.5877136


046
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.002661762
1.9762497


047
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.000473367
1.9764429


048
203414_at
monocyte to macrophage differentiation-associated
MMD
NM_012329
0.001585437
1.6128623


049
203553_s_at
mitogen-activated protein kinase kinase kinase kinase 5
MAP4K5
NM_006575
0.010453912
1.5251595


050
203924_at
glutathione S-transferase A1
GSTA1
NM_000846
0.004046575
4.2017674


051
203988_s_at
fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
FUT8
NM_004480
0.01139016
1.6090198


052
204426_at
transmembrane emp24 domain trafficking protein 2
TMED2
NM_006815
0.015985437
1.6165011


053
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.001788037
3.218731


054
204702_s_at
nuclear factor (erythroid-derived 2)-like 3
NFE2L3
NM_004289
0.015985157
1.7023398


055
204790_at
SMAD, mothers against DPP homolog 7 (Drosophila)
SMAD7
NM_005904
0.013379821
1.7179344


056
204944_at
protein tyrosine phosphatase, receptor type, G
PTPRG
NM_002841
0.004963213
1.769544


057
204989_s_at
integrin, beta 4
ITGB4
BF305661
0.012746719
2.1320713


058
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-associated glycoprotein)
SGCB
U29586
0.013908542
1.7317705


059
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.000473816
2.054043


060
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.003415823
2.4370956


061
206025_s_at
tumor necrosis factor, alpha-induced protein 6
TNFAIP6
AW188195
0.013965369
2.1515768


062
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.010821017
1.571063


063
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.000283653
2.0841253


064
206245_s_at
Influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.003607815
1.5105128


065
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.018292218
1.5056778


066
208510_s_at
peroxisome proliferative activated receptor, gamma
PPARG
NM_015869
0.002361554
1.882336


067
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.001033398
1.7958127


068
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.000448714
1.631627


069
208653_s_at
CD164 antigen, sialomucin
CD164
AF263279
0.017487219
1.5380286


070
208853_s_at
calnexin
CANX
L18887
0.011792572
1.5100785


071
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.001730693
1.8878508


072
209209_s_at
pleckstrin homology domain containing, family C
PLEKHC1
AW469573
0.009551367
1.9820172




(with FERM domain) member 1


073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.00507411
1.6641864


074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L
BC001465
0.006051209
1.6464524


075
209409_at
growth factor receptor-bound protein 10
GRB10
DB6962
0.01098607
1.7481923


076
209410_s_at
growth factor receptor-bound protein 10
GRB10
AF000017
0.013879589
1.701537


077
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.003979554
1.5687809


078
210845_s_at
plasminogen activator, urokinase receptor
PLAUR
U08839
0.007479298
1.7924315


079
210892_s_at
general transcription factor II, i
GTF2I
BC004472
0.003141172
1.619537


080
210933_s_at
fascin homolog 1, actin-bundling protein
FSCN1
BC004908
0.00342191
1.906748




(Strongylocentrotus purpuratus)


081
210987_x_at
tropomyosin 1 (alpha)
TPM1
M19267
0.004614187
1.6935222


082
211299_s_at
flotillin 2
FLOT2
BC003683
0.015057402
1.5387125


083
211506_s_at
interleukin 8
IL8
AF043337
0.005428782
2.867063


084
211559_s_at
cyclin G2
CCNG2
L49506
0.010491861
1.8367761


085
211599_x_at
met proto-oncogene (hepatocyte growth factor receptor)
MET
U19348
0.019789577
1.9247686


086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.000418344
1.997547


087
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.00240352
2.8568754


088
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.011889962
1.7860718


089
211924_s_at
plasminogen activator, urokinase receptor
PLAUR
AY029180
0.011789334
1.8189595


090
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.007531395
1.8490748


091
212012_at
peroxidasin homolog (Drosophila)
PXDN
BF342851
0.016265145
1.8463359


092
212660_at
PHD finger protein 15
PHF15
AI735639
0.007391165
1.5595657


093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.016607396
1.5904158


094
212907_at
Solute carrier family 30 (zinc transporter), member 1
SLC30A1
AI972416
0.002460855
1.63999


095
213288_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
AI761250
0.010427832
1.6232696


096
213457_at
malignant fibrous histiocytoma amplified sequence 1
MFHAS1
BF739959
0.003050241
1.8505166


097
213624_at
sphingomyelin phosphodiesterase, acid-like 3A
SMPDL3A
AA873600
0.005912889
1.8562527


098
213742_at
splicing factor, arginine/serine-rich 11
SFRS11
AW241752
0.006011819
1.9170463


099
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
5.50514E−05
1.5048952


100
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.015398935
1.5939685


101
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.003539713
1.8040004


102
214581_x_at
tumor necrosis factor receptor superfamily, member 21
TNFRSF21
BE568134
0.002274355
2.2189345


103
214701_s_at
fibronectin 1
FN1
AJ276395
0.001182322
2.071262


104
214866_at
plasminogen activator, urokinase receptor
PLAUR
X74039
0.003173471
1.7340106


105
214895_s_at
ADAM metallopeptidase domain 10
ADAM10
AU135154
0.004170008
1.9890832


106
215501_s_at
dual specificity phosphatase 10
DUSP10
AK022513
0.018290011
1.5388945


107
216035_x_at
transcription factor 7-like 2 (T-cell specific, HMG-box)
TCF7L2
AV721430
0.000657631
1.7091621


108
216511_s_at
transcription factor 7-like 2 (T-cell specific, HMG-box)
TCF7L2
AJ270770
0.004103699
1.5264177


109
216915_s_at
protein tyrosine phosphatase, non-receptor type 12
PTPN12
S69182
0.005493577
1.6935816


110
216971_s_at
plectin 1, intermediate filament binding protein 500 kDa
PLEC1
Z54367
0.01826363
1.7186335


111
217188_s_at
chromosome 14 open reading frame 1
C14orf1
AC007182
0.011925477
1.6185476


112
217448_s_at
chromosome 14 open reading frame 92
C14orf92
AL117508
0.007782524
1.5433311




similar to Epidermal Langerhans cell protein LCP1
LOC285412


113
217492_s_at
phosphatase and tensin homolog
PTEN
AF023139
0.007220107
1.5624946




(mutated in multiple advanced cancers 1)


114
218000_s_at
pleckstrin homology-like domain, family A, member 1
PHLDA1
NM_007350
0.016502094
1.6960312


115
218077_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
BE542551
0.01684034
1.5417765


116
218078_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
NM_016598
0.010970607
1.5836283


117
218435_at
DnaJ (Hsp40) homolog, subfamily C, member 15
DNAJC15
NM_013238
0.019865552
1.7292447


118
218644_at
pleckstrin 2
PLEK2
NM_016445
0.000675608
2.7071812


119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1
NM_006544
0.012352341
1.7368068


120
218815_s_at
transmembrane protein 51
TMEM51
NM_018022
0.000753902
1.6477742


121
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.009280122
1.6340361


122
218854_at
squamous cell carcinoma
SART2
NM_013352
0.014419112
1.6285655




antigen recognized by T cells 2


123
218856_at
tumor necrosis factor receptor superfamily, member 21
TNFRSF21
NM_016629
0.01292243
1.617686


124
218885_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT12
NM_024642
0.014052196
1.6402073




N-acetylgalactosaminyltransferase 12 (GalNAc-T12)


125
219410_at
transmembrane protein 45A
TMEM45A
NM_018004
0.018847797
2.0938365


126
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.005593323
1.5408667


127
220199_s_at
chromosome 1 open reading frame 80
C1orf80
NM_022831
0.016323
1.5315142


128
220617_s_at
zinc finger protein 532
ZNF532
NM_018181
0.001976648
1.5441327


129
221268_s_at
sphingosine-1-phosphate phosphatase 1
SGPP1
NM_030791
0.008873873
1.9432548


130
221881_s_at
chloride intracellular channel 4
CLIC4
AI638420
0.004401053
1.7742935


131
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.00011337
1.5270268


132
222449_at
tranamembrane, prostate
TMEPAI
AL035541
0.005303006
2.2757804




androgen induced RNA


133
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.014745607
1.738053


134
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.005694628
1.5068418


135
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.001075083
1.5835624


136
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.000622161
1.7766397


137
222773_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT12
AA554045
0.003090952
1.8790901




N-acetylgalactosaminyltransferase 12 (GalNAc-T12)


138
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.003659447
1.6790042


139
223940_x_at
metastasis associated lung adenocarcinoma
MALAT1
AF132202
0.016841894
1.9524238




transcript 1 (non-coding RNA)


140
224558_s_at
metastasis associated lung adenocarcinoma
MALAT1
AI446756
0.012874936
1.6367766




transcript 1 (non-coding RNA)


141
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.002428954
1.6452742


142
224733_at
chemokine-like factor superfamily 3
CKLFSF3
AL574900
0.013543638
1.5199631


143
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.013437813
1.5261155


144
225021_at
zinc finger protein 532
ZNF532
AA861416
0.002285053
1.6213596


145
225140_at
Kruppel-like factor 3 (basic)
KLF3
BF438116
0.016804362
1.5368354


146
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.006987929
1.5712297


147
225424_at
glycerol-3-phosphate acyltransferase, mitochondrial
GPAM
AB046780
0.000390623
1.7006425


148
225503_at
dehydrogenase/reductase (SDR family) X-linked
DHRSX
AL547782
0.005000754
1.770981


149
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.003047524
1.6990312


150
225609_at
glutathione reductase
GSR
AI888037
0.004693668
1.8490914


151
225842_at
Pleckstrin homology-like domain, family A, member 1
PHLDA1
AK026181
0.014052763
1.8735564


152
226084_at
microtubule-associated protein 1B
MAP1B
AA554833
0.016480966
1.9064581


153
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.001219355
1.5196482


154
226726_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
W63676
0.005363467
1.8277074


155
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.001859941
1.5185972


156
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.000646104
1.6094143


157
227628_at
similar to RIKEN cDNA 2310016C16
LOC493869
AL571557
0.006222301
2.0978951


158
227808_at
DnaJ (Hsp40) homolog, subfamily C, member 15
DNAJC15
AI091398
0.01153802
1.7936606


159
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.005127667
1.6694399


160
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.004784999
1.72546


161
231823_s_at
KIAA1295
KIAA1295
BG054798
0.002478401
1.5713933


162
235587_at
hypothetical protein LOC202781
LOC202781
BG400596
0.018314553
1.5202585


163
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.002645486
2.0540323


164
238558_at
Muscleblind-like (Drosophila)
MBNL1
AI445833
0.004576562
1.805269


165
238563_at
Ab1-interactor 1
ABI1
AV762916
0.012934915
1.6069295


166
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01719282
1.5133282









The gene expression pattern of the lung cancer cell classified into adenocarcinoma after lung cancer tissue removal operation is analyzed through a hybridization with the probe on the microarray, and a marker gene that is determined to have a difference in an expression level between a patient with lung cancer recurrence within one year (recurrence group) and a patient without lung cancer recurrence even after three years (non-recurrence group) is selected. The results are shown in Table 2 below. A total number of adenocarcinoma patients was 23. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 8, and the number of patients without lung cancer recurrent even after three years was 15.
The gene expression pattern of the lung cancer cell classified into squamous cell carcinoma after lung cancer tissue removal operation is analyzed through a hybridization with the probe on the microarray, and a marker gene that is determined to have a difference in an expression level between a patient with lung cancer recurrence within one year (recurrence group) and a patient without lung cancer recurrence even after three years (non-recurrence group) is selected. The results are shown in Table 3 below. A total number of squamous cell carcinoma patients was 37. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 11, and the number of patients without lung cancer recurrent even after three years was 26.













TABLE 2










T-test
Fold






Genbank
p-
change


NO
Probe Set ID
Gene Name
Gene Symbol
Accession #
value
(abs)





















001
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.01
5.339528


002
1553589_a_at
PDZK1 interacting protein 1
PDZK1IP1
NM_005764
0.02
3.608417


003
1553768_a_at
discoidln, CUB and LCCL domain containing 1
DCBLD1
NM_173674
0.01
1.9046342


004
1553928_at
ELMO domain containing 2
ELMOD2
NM_153702
0.02
1.7168769


005
1554327_a_at
calcium activated nucleotidase 1
CANT1
AF328554
0.02
1.6306834


006
1558685_a_at
hypothetical protein BC009467
LOC158980
BC009467
0.03
1.6841992


007
1559399_s_at
zinc finger, CCHC domain containing 10
ZCCHC10
BC015988
0.02
1.5219704


008
1568578_s_at
FGFR1 oncogene partner
FGFR1OP
BC037785
0.01
2.4856193


009
160020_at
matrix metallopeptidase 14 (membrane-inserted)
MMP14
Z48481
0.03
1.8354192


010
200730_s_at
protein tyrosine phosphatase type IVA, member 1
PTP4A1
BF576710
0.03
2.6575127


011
200733_s_at
protein tyrosine phosphatase type IVA, member 1
PTP4A1
U48296
0.02
1.5593889


012
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.02
1.6270655


013
200890_s_at
signal sequence receptor, alpha
SSR1
AW006345
0.01
1.8127153




(translocon-associated protein alpha)


014
200931_s_at
vinculin
VCL
NM_014000
0.01
1.7692009


015
201011_at
ribophorin I
RPN1
NM_002950
0.01
1.6075972


016
201106_at
glutathione peroxidase 4
GPX4
NM_002085
0.02
1.6833277




(phospholipid hydroperoxidase)


017
201143_s_at
eukaryotic translation initiation factot 2.
EIF2S1
BC002513
0.02
2.298374




subunit 1 alpha, 35 kDa


018
201207_at
tumor necrosis factor, alpha-induced protein 1 (endothelial)
TNFAIP1
NM_021137
0.01
1.6828994


019
201250_s_at
solute carrier family 2
SLC2A1
NM_006516
0.02
4.009399




(facilitated glucose transporter), member 1


020
201392_s_at
insulin-like growth factor 2 receptor
IGF2R
BG031974
0.02
1.6488191


021
201393_s_at
insulin-like growth factor 2 receptor
IGF2R
NM_000876
0.02
1.5784883


022
201456_s_at
BUB3 budding uninhibited by
BUB3
AU160695
0.01
1.7238452




benzimidazoles 3 homolog (yeast)


023
201458_s_at
BUB3 budding uninhibited by
BUB3
NM_004725
0.01
1.5530633




benzimidazoles 3 homolog (yeast)


024
201525_at
apolipoprotein D
APOD
NM_001647
0.03
4.186704


025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.01
3.2328043




(Strongylocentrotus purpuratus)


026
201631_s_at
immediate early response 3
IER3
NM_003897
0.01
3.0016828


027
201656_at
integrin, alpha 6
ITGA6
NM_000210
0.01
2.3616688


028
201700_at
cyclin D3
CCND3
NM_001760
0.02
1.6460308


029
202047_s_at
chromobox homolog 6
CBX6
AI458128
0.01
1.9611783


030
202048_s_at
chromobox homolog 6
CBX6
NM_014292
0.02
1.6010046


031
202086_at
myxovirus (influenza virus) resistance 1,
MX1
NM_002462
0.02
2.4754105




interferon-inducible protein p78 (mouse)


032
202130_at
RIO kinase 3 (yeast)
RIOK3
AA725102
0.01
1.6167943


033
202131_s_at
RIO kinase 3 (yeast)
RIOK3
NM_003831
0.02
1.7833867


034
202233_s_at
ubiquinol-cytochrome c reductase hinge protein
UQCRH
NM_006004
0.03
1.5353662


035
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.01
3.9229517


036
202293_at
stromal antigen 1
STAG1
AW168948
0.01
1.7993419


037
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.02
2.0231702


038
202696_at
oxidative-stress responsive 1
OXSR1
NM_005109
0.03
1.5418515


039
202816_s_at
synovial sarcoma translocation, chromosome 18
SS18
AW292882
0.01
2.0899003


040
202856_s_at
solute carrier family 16 (monocarboxylic acid transporters),
SLC16A3
NM_004207
0.01
2.8914852




member 3


041
202869_at
2′,5′-oligoadenylate synthetase 1, 40/46 kDa
OAS1
NM_016816
0.02
3.431309


042
202887_s_at
DNA-damage-inducible transcript 4
DDIT4
NM_019058
0.02
2.74081


043
202904_s_at
LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
LSM5
NM_012322
0.03
1.8907431


044
202934_at
hexokinase 2
HK2
AI761561
0.01
2.1517375


045
203072_at
myosin IE
MYO1E
NM_004998
0.01
2.039332


046
203177_x_at
transcription factor A, mitochondrial
TFAM
NM_003201
0.02
1.8601428


047
203256_at
cadherin 3, type 1, P-cadherin (placental)
CDH3
NM_001793
0.01
2.6757588


048
203287_at
ladinin 1
LAD1
NM_005558
0.03
1.9237865


049
203311_s_at
ADP-ribosylation factor 6
ARF6
M57763
0.02
1.9452083


050
203313_s_at
TGFB-induced factor (TALE family homeobox)
TGIF
NM_003244
0.01
1.5528815


051
203344_s_at
retinoblastoma binding protein 8
RBBP8
NM_002894
0.01
1.7286093


052
203395_s_at
hairy and enhancer of split 1, (Drosophila)
HES1
NM_005524
0.02
1.6101321


053
203430_at
heme binding protein 2
HEBP2
NM_014320
0.02
1.822933


054
203476_at
trophoblast glycoprotein
TPBG
NM_006670
0.03
2.0313597


055
203499_at
EPH receptor A2
EPHA2
NM_004431
0.01
2.4758015


056
203501_at
plasma glutamate carboxypeptidase
PGCP
NM_006102
0.02
1.742001


057
203535_at
S100 calcium binding protein A9 (calgranulin B)
S100A9
NM_002965
0.02
5.647521


058
203554_x_at
pituitary tumor-transforming 1
PTTG1
NM_004219
0.02
2.1384234


059
203642_s_at
COBL-like 1
COBLL1
NM_014900
0.02
1.7199888


060
203690_at
tubulin, gamma complex associated protein 3
TUBGCP3
NM_006322
0.01
1.6228286


061
203906_at
IQ motif and Sec7 domain 1
IQSEC1
AI652645
0.01
1.7168043


062
203964_at
N-myc (and STAT) interactor
NMI
NM_004688
0.01
1.8720082


063
203988_s_at
fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
FUT8
NM_004480
0.01
2.0948534


064
204136_at
collagen, type VII, alpha 1 (epidermolysis bullosa,
COL7A1
NM_000094
0.01
2.2071517




dystrophic, dominant and recessive)


065
204401_at
potassium intermediate/small conductance
KCNN4
NM_002250
0.01
3.260382




calcium-activated channel, subfamily N, member 4


066
204415_at
interferon, alpha-inducible protein (clone IFI-6-16)
G1P3
NM_022873
0.02
4.0747566


067
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.01
6.7313213




(melanoma growth stimulating activity, alpha)


068
204580_at
matrix metallopeptidase 12 (macrophage elastase)
MMP12
NM_002426
0.02
7.360193


069
204587_at
solute carrier family 25 (mitochondrial carrier, brain), member 14
SLC25A14
NM_003951
0.02
1.5086871


070
204616_at
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
UCHL3
NM_006002
0.03
1.8766123


071
204635_at
ribosomal protein S6 kinase, 90 kDa, polypeptide 5
RPS6KA5
NM_004755
0.01
1.853935


072
204747_at
interferon-induced protein with tetratricopeptide repeats 3
IFIT3
NM_001549
0.02
2.588765


073
204809_at
ClpX caseinolytic peptidase X homolog (E. coli)
CLPX
NM_006660
0.02
1.5264844


074
204857_at
MAD1 mitotic arrest deficient-like 1 (yeast)
MAD1L1
NM_003550
0.03
1.6594671


075
204875_s_at
GDP-mannose 4,6-dehydratase
GMDS
NM_001500
0.02
2.5758607


076
204990_s_at
integrin, beta 4
ITGB4
NM_000213
0.01
3.176456


077
205004_at
NF-kappaB repressing factor
NKRF
NM_017544
0.02
1.5878501


078
205016_at
transforming growth factor, alpha
TGFA
NM_003236
0.01
2.1914852


079
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-associated glycoprotein)
SGCB
U29586
0.01
2.5721073


080
205157_s_at
keratin 17
KRT17
NM_000422
0.01
5.252511


081
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.01
2.1361954


082
205202_at
protein-L-isoaspartate (D-aspartate) O-methyltransferase
PCMT1
NM_005389
0.01
1.5924072


083
205339_at
TAL1 (SCL) interrupting locus
SIL
NM_003035
0.02
2.043193


084
205455_at
macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
MST1R
NM_002447
0.02
2.835629


085
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.01
3.8200433


086
205518_s_at
cytidine monophosphate-N-acetylneuraminic acid
CMAH
NM_003570
0.01
2.596108




hydroxy (CMP-N-acetylneuraminate monooxygenase)


087
205945_at
interleukin 6 receptor
IL6R
NM_000565
0.03
1.8261979


088
206055_s_at
small nuclear ribonucleoprotein polypeptide A′
SNRPA1
NM_003090
0.01
1.5232844


089
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.01
2.3268037


090
206414_s_at
development and differentiation enhancing factor 2
DDEF2
NM_003887
0.01
2.089077


091
207079_s_at
mediator of RNA polymerase II transcription,
MED6
NM_005466
0.03
1.8905708




subunit 6 homolog (yeast)


092
207850_at
chemokine (C-X-C motif) ligand 3
CXCL3
NM_002090
0.02
4.294934


093
208091_s_at
EGFR-coamplified and overexpressed protein
ECOP
NM_030796
0.02
2.2340379


094
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.01
2.3647172


095
208636_at
Actinin, alpha 1
ACTN1
AI082078
0.01
1.8102713


096
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.01
2.062581


097
208819_at
RAB8A, member RAS oncogene family
RAB8A
BC002977
0.01
1.6729795


098
208840_s_at
Ras-GTPase activating protein
G3BP2
AU149503
0.02
1.8072606




SH3 domain-binding protein 2


099
208875_s_at
p21 (CDKN1A)-activated kinase 2
PAK2
BF796470
0.01
2.1095228


100
208876_s_at
p21 (CDKN1A)-activated kinase 2
PAK2
AI076186
0.02
1.6706929


101
208878_s_at
p21 (CDKN1A)-activated kinase 2
PAK2
AF092132
0.02
1.5662557


102
209022_at
stromal antigen 2
STAG2
AK026678
0.01
1.5019888


103
209025_s_at
synaptotagmin binding, cytoplasmic RNA interacting protein
SYNCRIP
AF037448
0.01
1.748127


104
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.01
2.2400491


105
209417_s_at
interferon-induced protein 35
IFI35
BC001356
0.02
1.9908478


106
209476_at
thioredoxin domain containing
TXNDC
AL080080
0.02
1.5641398


107
209487_at
RNA binding protein with multiple splicing
RBPMS
D84109
0.02
1.5929683


108
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.03
2.019564


109
209627_s_at
oxysterol binding protein-like 3
OSBPL3
AY008372
0.03
1.9842228


110
209791_at
peptidyl arginine deiminase, type II
PADI2
AL049569
0.02
1.5902214


111
210092_at
mago-nashi homolog,
MAGOH
AF067173
0.03
1.7290384




proliferation-associated (Drosophila)


112
210093_s_at
mago-nashi homolog, proliferation-associated (Drosophila)
MAGOH
AF067173
0.01
1.5214177


113
210104_at
mediator of RNA polymerase II transcription.
MED6
AF074723
0.01
1.7416326




subunit 6 homolog (yeast)


114
210273_at
BH-protocadherin (brain-heart)
PCDH7
AB006757
0.03
1.5068512


115
210933_s_at
fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
FSCN1
BC004908
0.01
2.660472


116
211160_x_at
actinin, alpha 1
ACTN1
M95178
0.01
1.6758434


117
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.03
4.548989


118
211737_x_at
pleiotrophin
PTN
BC005916
0.02
2.2613049




(heparin binding growth factor 8, neurite growth-promoting factor 1)


119
212203_x_at
interferon induced transmembrane protein 3 (1-8U)
IFITM3
BF338947
0.01
1.5134683


120
212221_x_at
iduronate 2-sulfatase (Hunter syndrome)
IDS
AV703259
0.01
1.8884305


121
212236_x_at
keratin 17
KRT17
Z19574
0.01
3.7909358


122
212268_at
serpin peptidase inhibitor, clade B (ovalbumin), member 1
SERPINB1
NM_030666
0.02
1.9949495


123
212312_at
BCL2-like 1
BCL2L1
AL117381
0.02
1.5705433


124
212322_at
sphingosine-1-phosphate lyase 1
SGPL1
BE999972
0.01
1.6549215


125
212330_at
transcription factor Dp-1
TFDP1
R60866
0.02
2.1620867


126
212531_at
lipacalin 2 (oncogene 24p3)
LCN2
NM_005564
0.02
6.2857018


127
212657_s_at
interleukin 1 receptor antagonist
IL1RN
U65590
0.02
3.7755005


128
212858_at
progestin and adipoQ receptor family member IV
PAQR4
AL520675
0.01
2.2580597


129
212992_at
chromosome 14 open reading frame 78
C14orf78
AI935123
0.01
5.9573503


130
213088_s_at
DnaJ (Hsp40) homolog, subfamily C, member 9
DNAJC9
BE551340
0.02
1.784215


131
213288_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
AI761250
0.02
2.1144574


132
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
0.01
1.7699668


133
214453_s_at
interferon-induced protein 44
IFI44
NM_006417
0.03
2.8858101


134
214697_s_at
ROD1 regulator of differentiation 1 (S. pombe)
ROD1
AW190873
0.01
2.048636


135
214974_x_at
chemokine (C-X-C motif) ligand 5
CXCL5
AK026546
0.02
6.4936213


136
215223_s_at
superoxide dismutase 2, mitochondrial
SOD2
W46388
0.01
3.1782749


137
215230_x_at
eukaryotic translation initiation factor 3, subunit 8, 110 kDa
EIF3S8
AA679705
0.02
1.6019442


138
215411_s_at
TRAF3 interacting protein 2
TRAF3IP2
AL008730
0.03
1.72815


139
216153_x_at
reversion-inducing-cysteine-rich
RECK
AK022897
0.01
1.9417262




protein with kazal motifs


140
216841_s_at
superoxide dismutase 2, mitochondrial
SOD2
X15132
0.01
2.8182118


141
216905_s_at
suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
ST14
U20428
0.02
1.8127093


142
216977_x_at
small nuclear ribonucleoprotein polypeptide A′
SNRPA1
AJ130972
0.01
1.5991035


143
217834_s_at
synaptotagmin binding, cytoplasmic RNA interacting protein
SYNCRIP
NM_006372
0.03
1.7178055


144
217867_x_at
beta-site APP-cleaving enzyme 2
BACE2
NM_012105
0.01
2.5611665


145
217901_at
Desmoglein 2
DSG2
BF031829
0.01
3.4549432


146
218012_at
TSPY-like 2
TSPYL2
NM_022117
0.01
1.6316599


147
218288_s_at
hypothetical protein MDS025
MDS025
NM_021825
0.01
1.7013886


148
218294_s_at
nucleoporin 50 kDa
NUP50
AF267865
0.01
1.5833666


149
218400_at
2′-5′-oligoadenylate synthetase 3, 100 kDa
OAS3
NM_006187
0.01
3.0217175


150
218451_at
CUB domain containing protein 1
CDCP1
NM_022842
0.01
3.0102131


151
218460_at
hypothetical protein FLJ20397
FLJ20397
NM_017802
0.02
1.6881874


152
218498_s_at
ERO1-like (S. cerevisiae)
ERO1L
NM_014584
0.01
2.5205412


153
218573_at
melanoma antigen family H, 1
MAGEH1
NM_014061
0.02
1.6212198


154
218585_s_at
denticleless homolog (Drosophila)
DTL
NM_016448
0.03
2.4223747


155
218644_at
pleckstrin 2
PLEK2
NM_016445
0.01
4.898943


156
218796_at
chromosome 20 open reading frame 42
C20orf42
NM_017671
0.02
3.3694396


157
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.03
2.0183008


158
218943_s_at
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
DDX58
NM_014314
0.02
2.4575703


159
218950_at
centaurin, delta 3
CENTD3
NM_022481
0.02
1.5173771


160
219146_at
chromosome 17 open reading frame 42
C17orf42
NM_024683
0.02
1.5234692


161
219296_at
zinc finger, DHHC-type containing 13
ZDHHC13
NM_019028
0.03
1.5033884


162
219303_at
chromosome 13 open reading frame 7
C13orf7
NM_024546
0.03
1.5534021


163
219332_at
MICAL-like 2
MICAL-L2
NM_024723
0.02
1.8410143


164
219399_at
lin-7 homolog C (C. elegans)
LIN7C
NM_018362
0.03
1.5852816


165
219421_at
osmosis responsive factor
OSRF
NM_012382
0.01
1.531867


166
219439_at
core 1 synthase, glycoprotein-N-acetylgalactosamine
C1GALT1
NM_020156
0.02
2.2143774




3-beta-galactosyltransferase, 1


167
219517_at
elongation factor RNA polymerase II-like 3
ELL3
NM_025165
0.02
1.6594616


168
219549_s_at
reticulon 3
RTN3
NM_006054
0.02
1.6491096


169
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.01
1.8911394


170
219630_s_at
PDZK1 interacting protein 1
PDZK1IP1
NM_005764
0.02
3.5720232


171
219691_at
sterile alpha motif domain containing 9
SAMD9
NM_017654
0.01
2.2009485


172
219787_s_at
epithelial cell transforming sequence 2 oncogene
ECT2
NM_018098
0.02
3.414079


173
219799_s_at
dehydrogenase/reductase (SDR family) member 9
DHRS9
NM_005771
0.02
1.7866958


174
219959_at
molybdenum cofactor sulfurase
MOCOS
NM_017947
0.01
3.192601


175
220232_at
stearoyl-CoA desaturase 5
SCD5
NM_024906
0.01
3.2719014


176
220368_s_at
KIAA2010
KIAA2010
NM_017936
0.02
1.6052217


177
220725_x_at
Dynein, axonemal, heavy polypeptide 3
DNAH3
NM_025095
0.01
1.8525391


178
221477_s_at
hypothetical protein MGC5618
MGC5618
BF575213
0.01
2.2014346


179
221482_s_at
cyclic AMP phosphoprotein, 19 kD
ARPP-19
BC003418
0.02
1.711658


180
221732_at
calcium activated nucleotidase 1
CANT1
AK026161
0.02
1.6711121


181
221752_at
Slingshot homolog 1 (Drosophila)
SSH1
AL041728
0.02
1.678051


182
221922_at
G-protein signalling modulator 2 (AGS3-like, C. elegans)
GPSM2
AW195581
0.01
2.2638144


183
222392_x_at
PERP, TP53 apoptosis effector
PERP
AJ251830
0.02
1.8814404


184
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.02
1.6986449


185
222424_s_at
nuclear casein kinase and cyclin-dependent kinase substrate 1
NUCKS1
BC000805
0.01
1.6469624


186
222446_s_at
beta-site APP-cleaving enzyme 2
BACE2
AF178532
0.01
1.9711965


187
222492_at
pyridoxal (pyridoxine, vitamin B6) kinase
PDXK
AW262867
0.01
1.5873553


188
222502_s_at
ubiquitin-fold modifier 1
UFM1
BC005193
0.02
1.7238611


189
222523_at
SUMO1/sentrin/SMT3 specific peptidase 2
SENP2
BE622841
0.03
1.7830018


190
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.02
2.6761055


191
222561_at
LanC lantibiotic synthetase component C-like 2 (bacterial)
LANCL2
AJ278245
0.03
2.2797666


192
222587_s_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT7
BF699855
0.03
1.7439753




N-acetylgalactosaminyltransferase 7(GalNAc-T7)


193
222689_at
phytoceramidase, alkaline
PHCA
N51263
0.01
1.7877864


194
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.01
1.9685304


195
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.02
2.1501522


196
222793_at
DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
DDX58
AK023661
0.01
2.2502613


197
223219_s_at
CCR4-NOT transcription complex, subunit 10
CNOT10
BC002931
0.01
1.5173706


198
223278_at
gap junction protein, beta 2, 26 kDa (connexin 26)
GJB2
M86849
0.02
5.1083236


199
223374_s_at
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 3
B3GALT3
AF154848
0.02
2.124231


200
223421_at
cysteine/histidine-rich 1
CYHR1
BC005073
0.01
1.7838429


201
223467_at
RAS, dexamethasone-induced 1
RASD1
AF069506
0.01
3.1274104


202
223626_x_at
family with sequence similarity 14, member A
FAM14A
AF208232
0.01
1.5701514


203
223631_s_at
chromosome 19 open reading frame 33
C19orf33
AF213678
0.02
3.90325


204
224159_x_at
tripartite motif-containing 4
TRIM4
AF220023
0.01
2.2881489


205
224493_x_at
chromosome 18 open reading frame 45
C18orf45
BC006280
0.02
1.571958


206
224494_x_at
dehydrogenase/reductase (SDR family) member 10
DHRS10
BC006283
0.02
1.9102337


207
224564_s_at
reticulon 3
RTN3
BE544689
0.01
1.583082


208
224595_at
solute carrier family 44, member 1
SLC44A1
AK022549
0.01
1.601491


209
224596_at
solute carrier family 44, member 1
SLC44A1
AI634866
0.01
1.5728544


210
224598_at
mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-
MGAT4B
BF570193
0.03
1.5535489




acetylglucosaminyltransferase, isoenzyme B


211
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.02
2.123153


212
224675_at
mesoderm development candidate 2
MESDC2
AK026606
0.01
1.6605617


213
224679_at
mesoderm development candidate 2
MESDC2
BE963495
0.01
1.65804


214
224681_at
guanine nucleotide binding protein (G protein) alpha 12
GNA12
BG028884
0.01
1.6103705


215
224799_at
Nedd4 family interacting protein 2
NDFIP2
AW290956
0.02
1.9774225


216
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.02
1.6960912


217
224827_at
Dendritic cell-derived ubiquitin-like protein
DC-UbP
AK022894
0.01
1.5073498


218
224902_at
pyruvate dehydrogenase phosphatase regulatory subunit
PDPR
BE644918
0.02
1.6357323


219
224950_at
prostaglandin F2 receptor negative regulator
PTGFRN
BF476250
0.03
1.9777663


220
225071_at
chromosome 6 open reading frame 68
C6orf68
BG168247
0.03
1.6909997


221
225272_at
spermidine/spermine N1-acetyltransferase 2
SAT2
AA128261
0.01
1.6911607


222
225331_at
chromosome 3 open reading frame 6
C3orf6
BF941088
0.02
2.126105


223
225342_at
adenylate kinase 3-like 1
AK3L1
AK026966
0.01
7.1160383


224
225366_at
phosphoglucomutase 2
PGM2
AI652855
0.03
1.527827


225
225375_at
chromosome 17 open reading frame 32
C17orf32
AW975808
0.02
1.8780395


226
225380_at
hypothetical protein BC007901
LOC91461
BF528878
0.02
2.6365216


227
225383_at
zinc finger protein 275
ZNF275
BF793625
0.01
1.639558


228
225547_at
HBII-276 host gene
HBII-276HG
BG169443
0.01
1.6269366


229
225550_at


AV700816
0.01
1.6167612


230
225571_at
leukemia inhibitory factor receptor
LIFR
AA701657
0.03
3.5799398


231
225575_at
leukemia inhibitory factor receptor
LIFR
AI680541
0.01
3.1433964


232
225578_at
similar to RIKEN cDNA 2410129H14
LOC440145
AI885466
0.01
1.8692675


233
225750_at
ERO1-like (S. cerevisiae)
ERO1L
BE966748
0.02
2.0413787


234
225842_at
Pleckstrin homology-like domain, family A, member 1
PHLDA1
AK026181
0.02
2.5619717


235
225847_at
arylacetamide deacetylase-like 1
AADACL1
AB037784
0.02
1.6796919


236
226060_at
RFT1 homolog (S. cerevisiae)
RFT1
BF475369
0.02
1.5211235


237
226112_at
sarcoglycan, beta (43 kDa dystrophin-associated glycoprotein)
SGCB
AI678717
0.01
1.5416645


238
226278_at
hypothetical protein DKFZp313A2432
DKFZp313A2432
AI150224
0.02
1.6910942


239
226335_at
ribosomal protein S6 kinase, 90 kDa, polypeptide 3
RPS6KA3
BG498334
0.01
1.8176109


240
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.01
2.4128768


241
226488_at
RCC1 domain containing 1
RCCD1
AW007826
0.03
1.777583


242
226568_at
hypothetical protein LOC284611
LOC284611
AI478747
0.01
2.1426997


243
226609_at
discoidin, CUB and LCCL domain containing 1
DCBLD1
N22751
0.01
2.0089936


244
226702_at
hypothetical protein LOC129607
LOC129607
AI742057
0.01
2.5539525


245
226722_at
family with sequence similarity 20, member C
FAM20C
BE874872
0.01
2.2937167


246
226726_at
O-acyltransferase (membrane bound) domain containing 2
OACT2
W63676
0.01
2.8518102


247
226778_at
Chromosome 8 open reading frame 42
C8orf42
AI632224
0.02
1.9250498


248
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.01
1.8384567


249
226781_at
hypothetical protein HSPC268
HSPC268
BF540829
0.01
1.7917764


250
226784_at
TWIST neighbor
TWISTNB
AA121481
0.01
1.750498


251
226832_at
Hypothetical LOC389188
LOC389188
BF978778
0.01
1.538109


252
226863_at
Full-length cDNA clone CS0DJ001YJ05 of T cells

AI674565
0.01
3.1555974




(Jurkat cell line) Cot 10-normalized of Homo sapiens (human)


253
226926_at
dermokine
ZD52F10
AA706316
0.02
3.190141


254
227141_at
chromosome 1 open reading frame 171
C1orf171
AW205739
0.02
1.6063374


255
227148_at
pleckstrin homology domain containing, family H
PLEKHH2
AI913749
0.03
2.1525955




(with MyTH4 domain) member 2


256
227172_at
hypothetical protein BC000282
LOC89894
BC000282
0.02
1.9858925


257
227249_at


AI857685
0.01
1.9229563


258
227314_at
Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
ITGA2
N95414
0.03
3.3500278


259
227393_at
transmembrane protein 16J
TMEM16J
AW084755
0.01
1.6880668


260
227466_at
hypothetical protein LOC285550
LOC285550
BF108695
0.02
1.5282669


261
227771_at
leukemia inhibitory factor receptor
LIFR
AW592684
0.01
2.7902896


262
227808_at
DnaJ (Hsp40) homolog, subfamily C, member 15
DNAJC15
AI091398
0.03
1.8649827


263
227998_at
S100 calcium binding protein A16
S100A16
AA045184
0.01
2.2575665


264
228152_s_at
hypothetical protein FLJ31033
FLJ31033
AK023743
0.02
2.2769616


265
228275_at
CDNA FLJ32438 fis,

AI200555
0.02
1.813842




clone SKMUS2001402


266
228531_at
sterile alpha motif domain containing 9
SAMD9
AA741307
0.02
2.303081


267
228562_at
Zinc finger and BTB domain containing 10
ZBTB10
N29918
0.01
2.046323


268
228600_x_at
hypothetical protein MGC72075
MGC72075
BE220330
0.02
1.6221175


269
228640_at
BH-protocadherin (brain-heart)
PCDH7
BE644809
0.03
3.3346767


270
228713_s_at
dehydrogenase/reductase (SDR family) member 10
DHRS10
AI742586
0.02
1.9451209


271
228854_at
Transcribed locus

AI492388
0.03
4.4617124


272
228972_at


AI028602
0.02
1.6522069


273
229573_at
Transcribed locus

AI659456
0.02
1.5438964


274
229582_at
chromosome 18 open reading frame 37
C18orf37
AI758919
0.01
1.6219943


275
229997_at
vang-like 1 (van gogh, Drosophila)
VANGL1
AA789332
0.02
1.6355668


276
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.01
1.7685658


277
230329_s_at
nudix (nucleoside diphosphate linked moiety X)-type motif 6
NUDT6
AI580268
0.02
1.5125636


278
230655_at
Homo sapiens, clone IMAGE: 5418468, mRNA

AW025928
0.01
2.44095


279
230972_at
ankyrin repeat domain 9
ANKRD9
AW194999
0.01
1.875526


280
231828_at
Homo sapiens, clone IMAGE: 5218355, mRNA

AL117474
0.02
2.1623232


281
231832_at
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
GALNT4
AI890347
0.01
1.8446548




N-acetylgalactosaminyltransferase 4 (GalNAc-T4)


282
234675_x_at
CDNA: FLJ23566 fis, clone LNG10880

AK027219
0.01
2.4514613


283
234725_s_at
sema domain, immunoglobulin domain (lg), transmembrane domain
SEMA4B
AK026133
0.01
1.9406958




(TM) and short cytoplasmic domain, (semaphorin) 4B


284
235015_at
Zinc finger, DHHC-type containing 9
ZDHHC9
AL529434
0.01
2.4835925


285
235019_at
carboxypeptidase M
CPM
BE878495
0.02
3.833762


286
235096_at
Leo1, Paf1/RNA polymerase II complex component,
LEO1
AA074729
0.01
1.5779704




homolog (S. cerevisiae)


287
235648_at
zinc finger protein 567
ZNF567
AA742659
0.02
1.6336213


288
235911_at
hypothetical gene supported by BC034933; BC068085
LOC440995
AI885815
0.01
4.651685


289
238063_at
hypothetical protein FLJ32028
FLJ32028
AA806283
0.01
2.002421


290
238523_at
chromosome 16 open reading frame 44
C16orf44
BF941204
0.03
1.5099897


291
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01
2.3077648


292
238778_at
membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7)
MPP7
AI244661
0.02
3.0538154


293
239896_at
Similar to RAB guanine nucleotide exchange factor (GEF) 1
LOC402671
AW190479
0.02
1.6268736


294
241994_at
Xanthine dehydrogenase
XDH
BG260086
0.02
3.2672102


295
241996_at


AI669591
0.01
1.7369617


296
244495_x_at
chromosome 18 open reading frame 45
C18orf45
AL521157
0.01
1.8056976


297
36553_at
acetylserotonin O-methyltransferase-like
ASMTL
AA669799
0.02
1.6164968


298
36829_at
period homolog 1 (Drosophila)
PER1
AF022991
0.01
1.9640467


299
55081_at
MICAL-like 1
MICAL-L1
W46406
0.02
1.5616423


300
60474_at
chromosome 20 open reading frame 42
C20orf42
AA469071
0.01
3.1548133






















TABLE 3











Fold






Genbank
T-test
change


NO.
Probe Set ID
Gene Name
Gene Symbol
Accession #
p-value
(abs)





















001
117_at
heat shock 70 kDa protein 6 (HSP70B$$)
HSPAB
X51757
0.03
1.7216957


002
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.02
1.5217854


003
1553530_a_at
integrin, beta 1 (fibronectin receptor,
ITGB1
NM_033669
0.01
2.0436814




beta polypeptide, antigen CD29 includes MDF2, MSK12)


004
1553694_a_at
phosphoinositide-3-kinase, class 2, alpha polypeptide
PIK3C2A
NM_002645
0.03
1.6315013


005
1553715_s_at
hypothetical protein MGC15416
MGC15416
NM_032371
0.02
1.5123988


006
1554747_a_at
septin 2
02-Sep
BC033559
0.01
1.560747


007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin gamma)
ADAM9
AF495383
0.03
2.140922


008
1555060_a_at
KIAA1702 protein
KIAA1702
AK027074
0.01
1.5686767


009
1557987_at
PI-3-kinase-related kinase SMG-1 - like locus
LOC641298
BC042832
0.01
2.2149343


010
1558678_s_at
metastasis associated lung adenocarcinoma transcript 1 (non-coding
MALAT1
BE708432
0.01
2.2265985




RNA)


011
1560622_at
TPA regulated locus
TPARL
AK000203
0.03
1.5656745


012
1564053_a_at
YTH domain family, member 3
YTHDF3
AK093081
0.02
1.8976958


013
1569106_s_at
hypothetical protein FLJ10707
FLJ10707
BI087313
0.02
1.5838199


014
200604_s_at
protein kinase, cAMP-dependent, regulatory, type I, alpha
PRKAR1A
M18468
0.02
1.5480618




(tissue specific extinguisher 1)


015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.01
1.5156919


016
200927_s_at
RAB14, member RAS oncogene family
RAB14
AA919115
0.01
1.607915


017
201152_s_at
muscleblind-like (Drosophila)
MBNL1
N31913
0.01
1.5028459


018
201194_at
selenoprotein W, 1
SEPW1
NM_003009
0.01
1.8139104


019
201362_at
influenza virus NS1A binding protein
IVNS1ABP
AF205218
0.02
1.5876002


020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.01
1.6949687


021
201376_s_at
heterogeneous nuclear ribonucleoprotein F
HNRPF
AI591354
0.01
1.5007194


022
201386_s_at
DEAH (Asp-Glu-Ala-His) box polypeptide 15
DHX15
AF279891
0.01
1.7872009


023
201399_s_at
translocation associated membrane protein 1
TRAM1
NM_014294
0.01
1.6199075


024
201505_at
laminin, beta 1
LAMB1
NM_002291
0.01
2.091507


025
201548_s_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
W02593
0.02
1.5838325


026
201549_x_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
NM_006618
0.02
1.6096623


027
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.02
2.2302318


028
201578_at
podocalyxin-like
PODXL
NM_005397
0.01
2.138917


029
201617_x_at
caldesmon 1
CALD1
NM_004342
0.02
2.0084002


030
201619_at
peroxiredoxin 3
PRDX3
NM_006793
0.01
1.5513384


031
201646_at
scavenger receptor class B, member 2
SCARB2
AA885297
0.02
1.6010221


032
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.03
1.5906466


033
201661_s_at
acyl-CoA synthetase long-chain family member 3
ACSL3
NM_004457
0.01
1.6001148


034
201678_s_at
DC12 protein
DC12
NM_020187
0.03
1.5643462


035
201787_at
fibulin 1
FBLN1
NM_001996
0.03
1.910708


036
201798_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
NM_013451
0.02
1.6354269


037
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.03
1.6411883


038
201942_s_at
carboxypeptidase D
CPD
D85390
0.02
1.6134206


039
202007_at
nidogen 1
NID1
BF940043
0.03
1.784865


040
202143_s_at
COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
COPS8
NM_006710
0.02
1.5126611


041
202374_s_at
RAB3 GTPase activating protein subunit 2 (non-catalytic)
RAB3GAP2
NM_012414
0.02
1.5766535


042
202429_s_at
protein phosphatase 3 (formerly 2B), catalytic subunit,
PPP3CA
AL353950
0.01
1.7161785




alpha isoform (calcineurin A alpha)


043
202444_s_at
SPFH domain family, member 1
SPFH1
NM_006459
0.01
1.8896967


044
202457_s_at
protein phosphatase 3 (formerly 2B), catalytic subunit,
PPP3CA
AA911231
0.01
1.552117




alpha isoform (calcineurin A alpha)


045
202536_at
chromatin modifying protein 2B
CHMP2B
AK002165
0.01
1.5160311


046
202593_s_at
membrane interacting protein of RGS16
MIR16
NM_016641
0.02
1.5102472


047
202627_s_at
serpin peptidase inhibitor, clade E
SERPINE1
AL574210
0.02
3.9358664




(nexin, plasminogen activator inhibitor type 1), member 1


048
202628_s_at
serpin peptidase inhibitor, clade E
SERPINE1
NM_000602
0.02
3.6850758




(nexin, plasminogen activator inhibitor type 1), member 1


049
202770_s_at
cyclin G2
CCNG2
NM_004354
0.03
1.5435082


050
202923_s_at
glutamate-cysteine ligase, catalytic subunit
GCLC
NM_001498
0.02
2.9063768


051
202946_s_at
BTB (POZ) domain containing 3
BTBD3
NM_014962
0.01
1.6240557


052
202955_s_at
ADP-ribosylation factor guanine nucleotide-exchange factor 1
ARFGEF1
AF084520
0.02
1.5484247




(brefeldin A-inhibited)


053
203066_at
B cell RAG associated protein
GALNAC4S-6ST
NM_014863
0.03
1.5839539


054
203085_s_at
transforming growth factor, beta 1 (Camurati-Engelmann disease)
TGFB1
BC000125
0.03
2.1608279


055
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.02
1.9789635


056
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.02
2.082541


057
203404_at
armadillo repeat containing. X-linked 2
ARMCX2
NM_014782
0.02
2.0663633


058
203748_x_at
RNA binding motif, single stranded interacting protein 1
RBMS1
NM_016839
0.01
1.6428717


059
204053_x_at
phosphatase and tensin homolog (mutated in multiple advanced
PTEN
U96180
0.02
1.7072555




cancers 1)


060
204066_s_at
centaurin, gamma 2
CENTG2
NM_014914
0.03
1.6650882


061
204605_at
cell growth regulator with ring finger domain 1
CGRRF1
NM_006568
0.02
1.5059351


062
204790_at
SMAD, mothers against DPP homolog 7 (Drosophila)
SMAD7
NM_005904
0.03
1.7849346


063
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.03
1.8976016


064
205436_s_at
H2A histone family, member X
H2AFX
NM_002105
0.01
1.542324


065
205527_s_at
gem (nuclear organelle) associated protein 4
GEMIN4
NM_015487
0.03
1.5615736


066
206042_x_at
small nuclear ribonucleoprotein polypeptide N SNRPN upstream
SNRPN
NM_022804
0.02
1.6762362




reading frame


067
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.02
1.7590842


068
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.01
2.168161


069
206245_s_at
influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.01
1.5090567


070
207266_x_at
RNA binding motif, single stranded interacting protein 1
RBMS1
NM_016837
0.01
1.6106415


071
207431_s_at
degenerative spermatocyte homolog 1, lipid desaturase (Drosophila)
DEGS1
NM_003676
0.01
1.542273


072
207821_s_at
PTK2 protein tyrosine kinase 2
PTK2
NM_005607
0.01
1.6032615


073
208097_s_at
thioredoxin domain containing
TXNDC
NM_030755
0.02
1.7288516


074
208643_s_at
X-ray repair complementing defective repair in Chinese hamster cells 5
XRCC5
J04977
0.02
1.5489099




(double-strand-break rejoining; Ku autoantigen, 80 kDa)


075
208859_s_at
alpha thalassemia/mental retardation syndrome X-linked
ATRX
AI650257
0.02
1.6250781




(RAD54 homolog, S. cerevisiae)


076
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.01
1.8967965


077
209209_s_at
pleckstrin homology domain containing, family C (with FERM domain)
PLEKHC1
AW469573
0.02
2.2543647




member 1


078
209409_at
growth factor receptor-bound protein 10
GRB10
D86962
0.02
1.7913702


079
209647_s_at
suppressor of cytokine signaling 5
SOCS5
AW664421
0.01
1.5314134


080
209868_s_at
RNA binding motif, single stranded interacting protein 1
RBMS1
D28482
0.01
1.757919


081
210154_at
malic enzyme 2, NAD(+)-dependent, mitochondrial
ME2
M55905
0.03
1.658911


082
210337_s_at
ATP citrate lyase
ACLY
U18197
0.03
1.6132175


083
210809_s_at
periostin, osteoblast specific factor
POSTN
D13665
0.03
1.9660459


084
211202_s_at
Jumonji, AT rich interactive domain 1B (RBP2-like)
JARID1B
AF087481
0.03
1.6053953


085
211559_s_at
cyclin G2
CCNG2
L49506
0.03
2.0475583


086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.01
2.44758


087
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.02
1.9618642


088
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.03
2.0343637


089
211985_s_at
calmodulin 1 (phosphorylase kinase, delta)
CALM1
AI653730
0.03
1.5034102


090
211992_at
WNK lysine deficient protein kinase 1
WNK1
AI445745
0.02
1.5539628


091
212298_at
neuropilin 1
NRP1
BE620457
0.02
1.7827071


092
212660_at
PHD finger protein 15
PHF15
AI735639
0.02
1.7572457


093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.02
1.6408824


094
212907_at
Solute carrier family 30 (zinc transporter), member 1
SLC30A1
AI972416
0.01
1.739024


095
213012_at
neural precursor cell expressed, developmentally down-regulated 4
NEDD4
D42055
0.02
1.6585234


096
213061_s_at
N-terminal asparagine amidase
NTAN1
AA643304
0.02
1.5069518


097
213901_x_at
RNA binding motif protein 9
RBM9
AW149379
0.02
1.5630468


098
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.02
1.8428509


099
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.02
1.8551272


100
214581_x_at
tumor necrosis factor receptor superfamily, member 21
TNFRSF21
BE568134
0.01
1.9035177


101
214701_s_at
fibronectin 1
FN1
AJ276395
0.01
2.180369


124
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.01
1.678279


125
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.02
1.5484349


126
223010_s_at
OCIA domain containing 1
OCIAD1
AA454649
0.01
1.638761


127
223110_at
KIAA1429
KIAA1429
BC003701
0.02
1.555597


128
223276_at
putative small membrane protein NID67
NID67
AF313413
0.02
1.8129323


129
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.02
2.037919


130
223940_x_at
metastasis associated lung adenocarcinoma transcript 1 (non-coding
MALAT1
AF132202
0.01
2.7140348




RNA)


131
224567_x_at
metastasis associated lung adenocarcinoma transcript 1 (non-coding
MALAT1
BG534952
0.02
2.436764




RNA)


132
224726_at
mindbomb homolog 1 (Drosophila)
MIB1
W80418
0.03
1.5452155


133
224819_at
transcription elongation factor A (SII)-like 8
TCEAL8
AI743979
0.01
1.5945034


134
224859_at
CD276 antigen
CD276
AL360136
0.03
1.5041374


135
225021_at
zinc finger protein 532
ZNF532
AA861416
0.02
1.6210703


136
225032_at
fibronectin type III domain containing 3B
FNDC3B
AI141784
0.01
1.5388452


137
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.01
1.8072422


138
225239_at


AI355441
0.02
2.2125103


139
225285_at
branched chain aminotransferase 1, cytosolic
BCAT1
AK025615
0.02
2.027126


140
225424_at
glycerol-3-phosphate acyltransferase, mitochondrial
GPAM
AB046780
0.02
1.740033


141
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.01
1.888815


142
225609_at
glutathione reductase
GSR
AI888037
0.02
2.144665


143
225974_at
transmembrane protein 64
TMEM64
BF732480
0.02
1.5707608


144
226280_at
BCL2/adenovirus E1B 19 kDa interacting protein 2
BNIP2
AA133277
0.02
1.5715192


145
226558_at
Full-length cDNA clone CS0DI062YC15 of Placenta Cot 25-normalized

BE856637
0.02
1.6961281




of Homo sapiens (human)


146
226675_s_at
metastasis associated, lung adenocarcinoma transcript 1 (non-coding
MALAT1
W80468
0.01
2.2176015




RNA)


147
226850_at
sulfatase modifying factor 1
SUMF1
AA683501
0.02
1.582926


148
227062_at
trophoblast-derived noncoding RNA
TncRNA
AU155361
0.01
3.1964853


149
227072_at
rotatin
RTTN
BG167480
0.02
1.6342819


150
227080_at
zinc finger protein 697
ZNF697
AW003092
0.01
2.047982


151
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.02
1.8308182


152
227456_s_at
chromosome 6 open reading frame 136
C6orf136
BF224092
0.02
1.5313978


153
229586_at
chromodomain helicase DNA binding protein 9
CHD9
AW300405
0.01
1.6146306


154
229606_at
Protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
PPP3CA
AI827550
0.02
1.5514666




(calcineurin A alpha)


155
229982_at
hypothetical protein FLJ21924
FLJ21924
AW195525
0.03
1.5703845


156
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.02
2.0209107


157
231823_s_at
KIAA1295
KIAA1295
BG054798
0.03
1.527874


158
234989_at
trophoblast-derived noncoding RNA
TncRNA
AV699657
0.02
2.0119648


159
235138_at
Pumilio homolog 2 (Drosophila)
PUM2
AA565051
0.01
1.7716993


160
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.01
2.2558458


161
236841_at
CXYorf1-related protein
FLJ25222
BE464132
0.01
1.7994804


162
238549_at
core-binding factor, runt domain, alpha subunit 2; translocated to, 2
CBFA2T2
AI420611
0.01
1.928193


163
239742_at
Tubby like protein 4
TULP4
H15278
0.03
1.5802637


164
242121_at


AW973232
0.03
1.7029374


165
243768_at
SUMO1/sentrin specific peptidase 6
SENP6
AA026388
0.01
2.2681193


166
244804_at
Sequestosome 1
SQSTM1
AW293441
0.01
1.5338039









In Tables 1, 2 and 3, gene name denotes a name of a gene, gene symbol denotes a symbol representing a gene, and Genbank Accession # denotes a number accessing Genbank which is a database that the public can access. T-test p value is obtained by statistically analyzing the degree of difference between an average expression level in a patient with lung cancer recurrence and an average expression level in a patient without lung cancer recurrence after lung cancer tissue removal operation.
Here, an expression level was calculated by Affymetrix GeneChip Operating Software (GCOS) Version 1.3 after a hybridization analysis using a microarray on which a probe is immobilized. Fold change (abs) indicates a ratio between an average expression level in a patient with lung cancer recurrence and an average expression level in a patient without lung cancer recurrence after lung cancer tissue removal operation in a hybridization analysis using a microarray on which a probe is immobilized.
As shown in Tables 1, 2 and 3, expression values of at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 showed statistically meaningful differences such that both T-test p values of the patient with lung cancer recurrence and the patient without lung cancer recurrence were less than 0.05. Therefore, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 can be used as a marker gene that can predict whether lung cancer is recurred afterwards with respect to the patients with a lung cancer removal operation. In addition, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 had showed that all the ratios of an expression average of the patients with lung cancer recurrence to an expression average of the patients without lung cancer recurrence was at least 1.5:1. Accordingly, it was confirmed that the expression of the marker gene was significantly increased in the patients with lung cancer recurrence.


DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described more specifically with reference to the following Examples. The following Examples are for illustrative purposes and are not intended to limit the scope of the invention.
EXAMPLE
Example 1
Selection of Marker Gene Related to Lung Cancer Recurrence
Primary lung cancer tissue having a tumor size of less than 3 cm and without lymph node metastase (that is, N0M0T1 stage) was collected. Total RNA was then immediately isolated from the collected lung cancer tissue. All the collected tumor tissue was lightly dyed with hematoxylin in order to improve visualization prior to RNA extraction. Each finely cut sample comprised at least 90% of tumor cells.
To avoid a necrotic region, one or two pieces of tumor tissue having a size of 5 mm×5 mm from the edge of tumor mass was immediately stored at −80□.
The finely cut tumor tissue was added to 1 ml of a Trizol reagent (Life Technologies, Rockville, Md.), and immediately homogenized by vortexing. Total RNA was isolated according to Trizol reagent protocol. The quality of the isolated total RNA was analyzed by electrophoresis using 1% agarose gel comprising 0.6 M formamide and ethidium bromide. An amount of total RNA was analyzed using a Nanodrop spectrometer (Nanodrop Technologies, Rockland, Del.).
The quality and amount of the isolated total RNA were confirmed to be excellent, and a reverse transcription reaction was performed using the RNA as a template and oligo dT as a primer to obtain cDNA. The obtained cDNA was used as a template that synthesizes cRNA through an in vitro transcription reaction. At this time, cRNA synthesized by adding UTP modified with biotin to a reaction solution was labeled with biotin. Next, the synthesized biotin-labeled cRNA was reacted with a hydroxyl radical to be fragmentized with a size of 50-200 bp. 10 μg of the fragmentized cRNA sample was injected onto an Affymetrix GeneChip array (human 133 plus ver 2) and hybridized at 45□ for 16 hours. The hybridization mixture was then removed and the microarrays were washed, stained with phycoerythrin-labeled Streptavidin, washed, incubated with biotinylated anti-streptavidin, and then restained with phycoerythrin-labeled Streptavidin to amplify the signals. Arrays were scanned using the GeneChip Scanner 3000 7G scanner (Affymetrix), controlled by Affymetrix GeneChip Operating System (GCOS) software. The Affymetrix Microarray Suite version 5 (MAS5) algorithm were utilized to analyze the hybridization intensity data from the microarrays and calculate a set of matrixes that describe probe set performance.
The obtained data was analyzed using an ArrayAssistTM (Stratagene, Inc., San Diego, USA) program. Data preprocessing was performed using a GCRMA (log 2 transformation) method that is a normalization method of multi-microarray level, in which fluorescence intensity values with respect to total microarrays used in analysis were substituted with log 2, and a fluorescence intensity average with respect to the total microarrays was adjusted taking into consideration of a GC amount of a nucleic acid sequence. Comparison between groups was performed under conditions of unpaired t-test, permutation=100, corrected p-value, Number of False Discovery Rate (NO/FDR). Data filtering was performed by selecting only data that satisfied an expression level (recurrence and non-recurrence, group average)>5 and fold change≧1.5. A count for each probeset_id was defined as the number of probe sets that showed a gene expression difference that satisfies the filtering standard in ADC, SQC, or in the recurrence group and non-recurrence group regardless of cell types.
As a result of analysis, the number of markers selected as positive expression with respect to adenocarcinoma (ADC) and squamous cell carcinoma (SQC) are shown in Table 4 below.











TABLE 4







total

squamous cell



lung cancer tissue
adenocarcinoma
carcinoma



















number of probe
166
300
166









Data related to expression of each gene that was obtained by the measurement of fluorescence intensity was obtained. To confirm correlation between the collected data related to expression of gene and lung cancer recurrence, patients with a lung cancer removal operation were monitored for five years to confirm lung cancer recurrence or non-recurrence. In the case of patients with lung cancer recurrence within one year after a lung cancer removal operation, they were grouped into a lung cancer recurrence group. In the case of patients without lung cancer recurrence even after three years after a lung cancer removal operation, they were grouped into a non-recurrence group. Data with respect to the obtained recurrence group and non-recurrence group among patients with a lung cancer removal operation was obtained.
Next, correlation between an expression pattern of each gene which was analyzed during the lung cancer removal operation, and the recurrence and non-recurrence groups that were subsequently obtained by monitoring the patients with a lung cancer removal operation was analyzed. The results are shown in Tables 1, 2 and 3.
Table 1 represents the results in which the gene expression pattern of the lung cancer cell after lung cancer tissue removal operation is analyzed through hybridization with a probe on a microarray, and a marker gene is selected, the marker gene being determined to have a difference in an expression level between a patient with lung cancer recurrence within one year and a patient without lung cancer recurrence even after three years. The total number of patients was 60. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 19, and the number of patients without lung cancer recurrent even after three years was 41.
Table 2 represents the results in which the gene expression pattern of the lung cancer cell which was classified into adenocarcinoma after lung cancer tissue removal operation is analyzed through hybridization with a probe on a microarray, and a marker gene is selected, the marker gene being determined to have a difference in an expression level between a patient with lung cancer recurrence within one year and a patient without lung cancer recurrence even after three years. A total number of adenocarcinoma patients was 23. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 8, and the number of patients without lung cancer recurrent even after three years was 15.
Table 3 represents the results in which the gene expression pattern of the lung cancer cell which was classified into squamous cell carcinoma after lung cancer tissue removal operation is analyzed through hybridization with a probe on a microarray, and a marker gene is selected, the marker gene being determined to have a difference in an expression level between a patient with lung cancer recurrence within one year and a patient without lung cancer recurrence even after three years. The total number of squamous cell carcinoma patients was 37. Among them, the number of patients with lung cancer recurrence within one year after lung cancer tissue removal operation was 11, and the number of patients without lung cancer recurrent even after three years was 26.
As shown in Tables 1, 2 and 3, expression values of at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 showed statistically meaningful differences such that both T-test p values of the patient with lung cancer recurrence and the patient without lung cancer recurrence were less than 0.05. Therefore, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 can be used as a marker gene that can predict whether lung cancer is likely to recur with respect to the patients that have had a lung cancer removal operation. In addition, at least one marker gene selected from the group consisting of marker genes of Genbank Accession No. shown in Tables 1, 2 and 3 showed that all the ratios of an expression average of the patients with lung cancer recurrence to an expression average of the patients without lung cancer recurrence were at least 1.5:1. Accordingly, it was confirmed that the expression of the marker gene was significantly increased in the patients with lung cancer recurrence.
The relationships between lung cancer recurrence in patients after lung cancer removal operation and conditions of the patients such as age, sex, smoking, cell type, pstage, and tumor size were analyzed, and the results are shown in Tables 5, 6 and 7.









TABLE 5





variation
statistical analysis method
result







sex
chi-square test
no difference: p value = 0.552


age
2-sample t-test
no difference: p value = 0.559


smoking
chi-square test
no difference: p value = 0.813


cell type
chi-square test
no difference: p value = 0.682


pstage
Fisher's exact test
no difference: p value = 0.305


tumor size
2-sample t-test
difference: p value = 0.039


metastasis
—
no metastasis









Table 5 shows results of analyzing 60 patients without classifying them according to cell types of lung cancer. Among 60 patients, the number of patients with lung cancer recurrence was 19, and the number of patients without lung cancer recurrence was 41. As shown in Table 5, the clinical indexes from the all patients looked no statistically meaningful difference in the recurrence group and the non-recurrence group. That is, the analyzed result can be regarded as a gene list that represents statistically meaningful difference in expression only with respect to the recurrence.









TABLE 6





variation
statistical analysis method
result







sex
Fisher's exact test
no difference: p value = 1.000


age
2-sample t-test
no difference: p value = 0.618


smoking
chi-square test
no difference: p value = 0.6570


cell type
—
adenocarcinoma (ADC)


pstage
Fisher's exact test
no difference: p value = 0.085


tumor size
2-sample t-test
no difference: p value = 0.051


metastasis
—
no metastasis









Table 6 shows results of analyzing 23 patients having adenocarcinoma when they are classified according to cell types of lung cancer. Among 23 patients, the number of patients with lung cancer recurrence was 8, and the number of patients without lung cancer recurrence was 15. As shown in Table 6, clinical information except the recurrence and tumor size which may induce confounding in other analysis may not have any statistically meaningful difference in the recurrence group and the non-recurrence group. That is, the analyzed result can be regarded as a gene list that represents statistically meaningful difference in expression only with respect to the recurrence.









TABLE 7





variation
statistical analysis method
result







sex
—
man


age
2-sample t-test
no difference: p value = 0.328


smoking
chi-square test
no difference: p value = 1.000


cell type
—
squamous cell carcinoma




(SQC)


pstage
Fisher's exact test
no difference: p value = 1.000


tumor size
2-sample t-test
no difference: p value = 0.417


metastasis
—
no metastasis









Table 7 shows results of analyzing 37 patients having squamous cell carcinoma when they are classified according to cell types of lung cancer. Among 23 patients, the number of patients with lung cancer recurrence was 11, and the number of patients without lung cancer recurrence was 26. As shown in Table 7, clinical information except the recurrence and tumor size which may induce confounding in other analysis may not have any statistically meaningful difference in the recurrence group and the non-recurrence group. That is, the analyzed result can be regarded as a gene list that represents statistically meaningful difference in expression only with respect to the recurrence.
Example 2
Prediction of Risk of Lung Cancer Recurrence Using Statistical Model
Based on the expression level of marker genes collected from the patients with lung cancer recurrence and non-recurrence which were obtained in Example 1, it was confirmed whether a risk of lung cancer recurrence could be predicted using a statistical analysis model.
In the analysis, a portion of each of data obtained with respect to total lung cancer tissue, adenocarcinoma and squamous cell carcinoma was used as a learning set to establish a basis on the prediction accuracy of the statistical model, the other portion of the data was used to identify whether the establish prediction accuracy is actually accurate using the leaning set
Data of learning sets and test sets with respect to the total lung cancer tissue, adenocarcinoma and squamous cell carcinoma are shown in Tables 8, 9 and 10.












TABLE 8







total lung cancer tissue
non-recurrence
recurrence
total





















learning set
28
15
43



test set
13
4
17



total
41
19
60






















TABLE 9







adenocarcinoma
non-recurrence
recurrence
total





















learning set
9
6
15



test set
6
2
8



total
16
8
23






















TABLE 10







squamous cell






carcinoma
non-recurrence
recurrence
total





















learning set
17
7
24



test set
9
4
13



total
26
11
37










Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a QDA prediction model are shown in Tables 11, 12 and 13 below. As shown in Tables 11, 12 and 13, the overall accuracy was at least 76.4%.







TABLE 11







Predicted results of the total lung cancer tissue using a QDA


prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
10
1
11



recurrence
3
3
6








overall accuracy
76.4%









The overall accuracy in Table 11 is a percentage of predicted class which corresponds to true class per total sample. That is, the overall accuracy is (17−4)×100/17=76.4%. The total is calculated in the same manner described above.







TABLE 12







Predicted results of adenocarcinoma tissue using


a QDA prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
6
0
6



recurrence
0
2
2








overall accuracy
100%
















TABLE 13







Predicted results of squamous cell carcinoma


tissue using a QDA prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
9
2
11



recurrence
0
2
2








overall accuracy
84.6%









Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Linear Discrimination Analysis (LDA) prediction model are shown in Tables 14, 15 and 16 below. As shown in Tables 14, 15 and 16, the overall accuracy was at least 76.4%.







TABLE 14







Predicted results of the total lung cancer tissue using a LDA


prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
10
1
11



recurrence
3
3
6








overall accuracy
76.4%
















TABLE 15







Predicted results of adenocarcinoma tissue using a LDA prediction


model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
6
0
6



recurrence
0
2
2








overall accuracy
100% 
















TABLE 16







Predicted results of squamous cell carcinoma tissue using a LDA


prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
9
1
10



recurrence
0
3
3








overall accuracy
92.3%









Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Neural network prediction model are shown in Tables 17, 18 and 19 below. As shown in Tables 17, 18 and 19, the overall accuracy was at least 59.46%.







TABLE 17







Predicted results of the total lung cancer tissue using a Neural


network prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
40
1
41



recurrence
18
1
19








overall accuracy
68.33%
















TABLE 18







Predicted results of adenocarcinoma tissue using a Neural


network prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
14
1
15



recurrence
1
7
8








overall accuracy
91.3%
















TABLE 19







Predicted results of squamous cell carcinoma tissue using


a Neural network prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
20
6
26



recurrence
9
2
11








overall accuracy
59.46%









Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Decision tree prediction model are shown in Tables 20, 21 and 22 below. As shown in Tables 20, 21 and 22, the overall accuracy was at least 61.67%.







TABLE 20







Predicted results of the total lung cancer tissue using a Decision


tree prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
35
6
41



recurrence
17
2
19








overall accuracy
61.67%
















TABLE 21







Predicted results of adenocarcinoma tissue using a Decision tree


prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
15
0
15



recurrence
8
0
8








overall accuracy
65.22%
















TABLE 22







Predicted results of squamous cell carcinoma tissue using a


Decision tree prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
25
1
26



recurrence
2
9
11








overall accuracy
91.89%









Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Support vector machine prediction model are shown in Tables 23, 24 and 25 below. As shown in Tables 23, 24 and 25, the overall accuracy was at least 65%.







TABLE 23







Predicted results of the total lung cancer tissue using a Support


vector machine prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
37
4
41



recurrence
17
2
19








overall accuracy
   65%
















TABLE 24







Predicted results of adenocarcinoma tissue using a Support vector


machine prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
15
0
15



recurrence
1
7
8








overall accuracy
95.65%
















TABLE 25







Predicted results of squamous cell carcinoma tissue using a


Support vector machine prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
24
2
26



recurrence
1
10
11








overall accuracy
91.89%









Results of predicting the test set with respect to the lung cancer tissue, adenocarcinoma and squamous cell carcinoma using a Naive Bayes prediction model are shown in Tables 26, 27 and 28 below. As shown in Tables 26, 27 and 28, the overall accuracy was at least 58.33%.







TABLE 26







Predicted results of the total lung cancer tissue using a Naive Bayes


prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
26
15
41



recurrence
10
9
19








overall accuracy
58.33%
















TABLE 27







Predicted results of adenocarcinoma tissue using a Naive Bayes


prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
15
0
15



recurrence
1
7
8








overall accuracy
95.65%
















TABLE 28







Predicted results of squamous cell carcinoma tissue using a Naive


Bayes prediction model










predicted class











classification
non-recurrence
recurrence
total














true class
non-recurrence
24
2
26



recurrence
1
10
11








overall accuracy
91.89%









The prediction models utilized in Examples of the present invention could have been easily understood by one of ordinary skill in the art (SAS Language: Reference, Version 6, First Edition by the SAS Institute.).
According to the method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment according to the present invention, the risk of lung cancer recurrence in a lung cancer patient after a lung cancer removal operation can be predicted with high accuracy.
According to the method of preparing a report on the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment according to the present invention, the report can be prepared to include results predicting the risk of lung cancer recurrence in a lung cancer patient after a lung cancer removal operation with high accuracy.
The report on the risk of lung cancer recurrence in a lung cancer patient or after the patient has lung cancer treatment according to the present invention includes highly accurate results predicting the risk of lung cancer recurrence in a lung cancer patient after a lung cancer removal operation.
According to the composition, kit and microarray for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment according to the present invention, diagnosis efficiency of risk of lung cancer recurrence of a lung cancer patient after a lung cancer treatment can be increased.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the following claims.

## Claims

1. A method of predicting risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the method comprising:
obtaining a lung cancer tissue sample from a lung cancer patient;
measuring an expression level in the lung cancer tissue sample of each target nucleic acid in a set of target nucleic acids consisting of target nucleic acids in Table 1;
determining whether the expression levels of the set of target nucleic acids in the lung cancer tissue sample correspond to expression levels of the set of target nucleic acids in a group of patients with lung cancer recurrence within one year after a lung cancer removal operation (“recurrence group”) or to expression levels of the set of target nucleic acids in a group of patients without lung cancer recurrence within three years after a lung cancer removal operation (“non-recurrence group”); and
predicting the risk of lung cancer recurrence in the patient is high when the expression levels of the set of target nucleic acids in the lung cancer tissue sample correspond to the expression levels of the recurrence group, or
predicting the risk of lung cancer recurrence in the patient is low when the expression levels of the set of target nucleic acids in the lung cancer tissue sample correspond to the expression levels of the nonrecurrence group,
wherein Table 1 is shown below
















No.
Probe Set ID
Gene Name
Gene Symbol





001
1552486_s_at
lactamase, beta
LACTB


002
1553105_s_at
desmoglein 2
DSG2


003
1553530_a_at
integrin, beta 1 (fibronectin
ITGB1




receptor, beta polypeptide,




antigen CD29 includes MDF2




MSK12)


004
1553678_a_at
integrin, beta 1 (fibronectin
ITGB1




receptor, beta polypeptide,




antigen CD29 includes




MDF2, MSK12)


005
1554067_at
hypothetical protein FLJ32549
FLJ32549


006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397


007
1555326_a_at
ADAM metallopeptidase
ADAM9




domain 9 (meltrin gamma)


008
1555564_a_at
I factor (complement)
IF


009
1555705_a_at
chemokine-like factor super-
CKLFSF3




family 3


010
1557987_at
PI-3-kinase-related kinase
LOC641298




SMG-1-like locus


011
1558678_s_at
metastasis associated lung
MALAT1




adenocarcinoma transcript 1




(non-coding RNA)


012
160020_at
matrix metallopeptidase 14
MMP14




(membrane-inserted)


013
200604_s_at
protein kinase, cAMP-
PRKAR1A




dependent, regulatory,




type I, alpha (tissue specific




extinguisher 1)


014
200615_s_at
adaptor-related protein
AP2B1




complex 2, beta 1 subunit


015
200864_s_at
RAB11A, member RAS
RAB11A




oncogene family


016
200922_at
KDEL (Lys-Asp-Glu-Leu)
KDELR1




endoplasmic reticulum




protein retention receptor 1


017
201020_at
tyrosine 3-monooxygenase/
YWHAH




tryptophan 5-monooxygenase




activation protein, eta




polypeptide


018
201179_s_at
guanine nucleotide binding
GNAI3




protein (G protein), alpha




inhibiting activity polypeptide




3


019
201309_x_at
chromosome 5 open reading
C5orf13




frame 13


020
201363_s_at
influenza virus NS1A binding
IVNS1ABP




protein


021
201505_at
laminin, beta 1
LAMB1


022
201506_at
transforming growth factor,
TGFBI




beta-induced, 68 kDa


023
201548_s_at
Jumonji, AT rich interactive
JARID1B




domain 1B (RBP2-like)


024
201559_s_at
chloride intracellular channel
CLIC4




4


025
201564_s_at
fascin homolog 1, actin-
FSCN1




bundling protein




(Strongylocentrotus




purpuratus)


026
201578_at
podocalyxin-like
PODXL


027
201617_x_at
caldesmon 1
CALD1


028
201646_at
scavenger receptor class B,
SCARB2




member 2


029
201647_s_at
scavenger receptor class B,
SCARB2




member 2


030
201695_s_at
nucleoside phosphorylase
NP


031
201722_s_at
UDP-N-acetyl-alpha-D-
GALNT1




galactosamine:polypeptide




N-acetylgalactosaminyltrans-




ferase 1 (GalNAc-T1)


032
201918_at
Solute carrier family 25,
SLC25A36




member 36


033
201942_s_at
carboxypeptidase D
CPD


034
202267_at
laminin, gamma 2
LAMC2


035
202543_s_at
glia maturation factor, beta
GMFB


036
202604_x_at
ADAM metallopeptidase
ADAM10




domain 10


037
202627_s_at
serpin peptidase inhibitor,
SERPINE1




clade E (nexin, plasminogen




activator inhibitor type 1),




member 1


038
202628_s_at
serpin peptidase inhibitor,
SERPINE1




clade E (nexin, plasminogen




activator inhibitor type 1),




member 1


039
202817_s_at
synovial sarcoma transloca-
SS18




tion, chromosome 18


040
202859_x_at
interleukin 8
IL8


041
202936_s_at
SRY (sex determining region
SOX9




Y)-box 9




(campomelic dysplasia,




autosomal sex-reversal)


042
202949_s_at
four and a half LIM domains
FHL2




2


043
202998_s_at
lysyl oxidase-like 2
LOXL2


044
203066_at
B cell RAG associated protein
GALNAC4S-6ST


045
203072_at
myosin IE
MYO1E


046
203293_s_at
lectin, mannose-binding, 1
LMAN1


047
203294_s_at
lectin, mannose-binding, 1
LMAN1


048
203414_at
monocyte to macrophage
MMD




differentiation-associated


049
203553_s_at
mitogen-activated protein
MAP4K5




kinase kinase kinase kinase 5


050
203924_at
glutathione S-transferase A1
GSTA1


051
203988_s_at
fucosyltransferase 8 (alpha
FUT8




(1,6) fucosyltransferase)


052
204426_at
transmembrane emp24
TMED2




domain trafficking protein 2


053
204470_at
chemokine (C-X-C motif)
CXCL1




ligand 1




(melanoma growth stimulating




activity, alpha)


054
204702_s_at
nuclear factor (erythroid-
NFE2L3




derived 2)-like 3


055
204790_at
SMAD, mothers against
SMAD7




DPP homolog 7 (Drosophila)


056
204944_at
protein tyrosine phosphatase,
PTPRG




receptor type, G


057
204989_s_at
integrin, beta 4
ITGB4


058
205120_s_at
sarcoglycan, beta (43 kDa
SGCB




dystrophin-associated




glycoprotein)


059
205180_s_at
ADAM metallopeptidase
ADAM8




domain 8


060
205479_s_at
plasminogen activator,
PLAU



urokinase


061
206025_s_at
tumor necrosis factor, alpha-
TNFAIP6




induced protein 6


062
206113_s_at
RAB5A, member RAS
RAB5A




oncogene family


063
206116_s_at
tropomyosin 1 (alpha)
TPM1


064
206245_s_at
Influenza virus NS1A binding
IVNS1ABP




protein


065
206323_x_at
oligophrenin 1
OPHN1


066
208510_s_at
peroxisome proliferative
PPARG




activated receptor, gamma


067
208613_s_at
filamin B, beta (actin binding
FLNB




protein 278)


068
208637_x_at
actinin, alpha 1
ACTN1


069
208653_s_at
CD164 antigen, sialomucin
CD164


070
208853_s_at
calnexin
CANX


071
209131_s_at
synaptosomal-associated
SNAP23




protein, 23 kDa


072
209209_s_at
pleckstrin homology domain
PLEKHC1




containing, family C




(with FERM domain) member




1


073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L


074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L


075
209409_at
growth factor receptor-bound
GRB10




protein 10


076
209410_s_at
growth factor receptor-bound
GRB10




protein 10


077
209537_at
exostoses (multiple)-like 2
EXTL2


078
210845_s_at
plasminogen activator,
PLAUR




urokinase receptor


079
210892_s_at
general transcription factor II,
GTF2I




i


080
210933_s_at
fascin homolog 1, actin-
FSCN1




bundling protein




(Strongylocentrotus




purpuratus)


081
210987_x_at
tropomyosin 1 (alpha)
TPM1


082
211299_s_at
flotillin 2
FLOT2


083
211506_s_at
interleukin 8
IL8


084
211559_s_at
cyclin G2
CCNG2


085
211599_x_at
met proto-oncogene
MET




(hepatocyte growth




factor receptor)


086
211651_s_at
laminin, beta 1
LAMB1


087
211668_s_at
plasminogen activator,
PLAU




urokinase


088
211864_s_at
fer-1-like 3, myoferlin (C.
FER1L3




elegans)


089
211924_s_at
plasminogen activator,
PLAUR




urokinase receptor


090
211981_at
collagen, type IV, alpha 1
COL4A1


091
212012_at
peroxidasin homolog
PXDN




(Drosophila)


092
212660_at
PHD finger protein 15
PHF15


093
212720_at
poly(A) polymerase alpha
PAPOLA


094
212907_at
Solute carrier family 30 (zinc
SLC30A1




transporter), member 1


095
213288_at
O-acyltransferase (membrane
OACT2




bound) domain containing 2


096
213457_at
malignant fibrous histio-
MFHAS1




cytoma amplified sequence 1


097
213624_at
sphingomyelin
SMPDL3A




phosphodiesterase, acid-




like 3A


098
213742_at
splicing factor, arginine/
SFRS11




serine-rich 11


099
214121_x_at
PDZ and LIM domain 7
PDLIM7




(enigma)


100
214196_s_at
tripeptidyl peptidase I
TPP1


101
214544_s_at
synaptosomal-associated
SNAP23




protein, 23 kDa


102
214581_x_at
tumor necrosis factor receptor
TNFRSF21




superfamily, member 21


103
214701_s_at
fibronectin 1
FN1


104
214866_at
plasminogen activator,
PLAUR




urokinase receptor


105
214895_s_at
ADAM metallopeptidase
ADAM10




domain 10


106
215501_s_at
dual specificity phosphatase
DUSP10




10


107
216035_x_at
transcription factor 7-like 2
TCF7L2




(T-cell specific, HMG-box)


108
216511_s_at
transcription factor 7-like 2
TCF7L2




(T-cell specific, HMG-box)


109
216915_s_at
protein tyrosine phosphatase,
PTPN12




non-receptor type 12


110
216971_s_at
plectin 1, intermediate
PLEC1




filament binding protein




500 kDa


111
217188_s_at
chromosome 14 open reading
C14orf1




frame 1


112
217448_s_at
chromosome 14 open reading
C14orf92




frame 92 similar to
LOC285412




Epidermal Langerhans




cell protein LCP1


113
217492_s_at
phosphatase and tensin
PTEN




homolog




(mutated in multiple advanced




cancers 1)


114
218000_s_at
pleckstrin homology-like
PHLDA1




domain, family A, member 1


115
218077_s_at
zinc finger, DHHC-type
ZDHHC3




containing 3


116
218078_s_at
zinc finger, DHHC-type
ZDHHC3




containing 3


117
218435_at
DnaJ (Hsp40) homolog,
DNAJC15




subfamily C, member 15


118
218644_at
pleckstrin 2
PLEK2


119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1


120
218815_s_at
transmembrane protein 51
TMEM51


121
218826_at
solute carrier family 35,
SLC35F2




member F2


122
218854_at
squamous cell carcinoma
SART2




antigen recognized by T




cells 2


123
218856_at
tumor necrosis factor receptor
TNFRSF21




superfamily, member 21


124
218885_s_at
UDP-N-acetyl-alpha-D-
GALNT12




galactosamine:polypeptide




N-acetylgalactosaminyl-




transferase 12 (GalNAc-T12)


125
219410_at
transmembrane protein 45A
TMEM45A


126
219603_s_at
zinc finger protein 226
ZNF226


127
220199_s_at
chromosome 1 open reading
C1orf80




frame 80


128
220617_s_at
zinc finger protein 532
ZNF532


129
221268_s_at
sphingosine-1-phosphate
SGPP1




phosphatase 1


130
221881_s_at
chloride intracellular
CLIC4




channel 4


131
222399_s_at
SM-11044 binding protein
SMBP


132
222449_at
tranamembrane, prostate
TMEPAI




androgen induced RNA


133
222528_s_at
solute carrier family 25,
SLC25A37




member 37


134
222540_s_at
hepatitis B virus x associated
HBXAP




protein


135
222692_s_at
fibronectin type III domain
FNDC3B




containing 3B


136
222693_at
fibronectin type III domain
FNDC3B




containing 3B


137
222773_s_at
UDP-N-acetyl-alpha-D-
GALNT12




galactosamine:polypeptide




N-acetylgalactosaminyl-




transferase 12 (GalNAc-T12)


138
223577_x_at
PRO1073 protein
PRO1073


139
223940_x_at
metastasis associated lung
MALAT1




adenocarcinoma transcript




1 (non-coding RNA)


140
224558_s_at
metastasis associated lung
MALAT1




adenocarcinoma transcript




1 (non-coding RNA)


141
224674_at
tweety homolog 3
TTYH3




(Drosophila)


142
224733_at
chemokine-like factor super-
CKLFSF3




family 3


143
224802_at
Nedd4 family interacting
NDFIP2




protein 2


144
225021_at
zinc finger protein 532
ZNF532


145
225140_at
Kruppel-like factor 3 (basic)
KLF3


146
225168_at
FERM domain containing 4A
FRMD4A


147
225424_at
glycerol-3-phosphate
GPAM




acyltransferase, mitochondrial


148
225503_at
dehydrogenase/reductase
DHRSX




(SDR family) X-linked


149
225567_at
Hypothetical LOC388114
LOC388114


150
225609_at
glutathione reductase
GSR


151
225842_at
Pleckstrin homology-like
PHLDA1




domain, family A, member 1


152
226084_at
microtubule-associated
MAP1B




protein 1B


153
226352_at
Junction-mediating and
JMY




regulatory protein


154
226726_at
O-acyltransferase (membrane
OACT2




bound) domain containing 2


155
226780_s_at
hypothetical protein HSPC268
HSPC268


156
227257_s_at
chromosome 10 open reading
C10orf46




frame 46


157
227628_at
similar to RIKEN cDNA
LOC493869




2310016C16


158
227808_at
DnaJ (Hsp40) homolog,
DNAJC15




subfamily C, member 15


159
230206_at
Dedicator of cytokinesis 5
DOCK5


160
231735_s_at
PRO1073 protein
PRO1073


161
231823_s_at
KIAA1295
KIAA1295


162
235587_at
hypothetical protein
LOC202781




LOC202781


163
235879_at
Muscleblind-like (Drosophila)
MBNL1


164
238558_at
Muscleblind-like (Drosophila)
MBNL1


165
238563_at
Ab1-interactor 1
ABI1


166
238701_x_at
FLJ45803 protein
FLJ45803













No.
Genbank Accession #
T-test p-value
Fold change (abs)













001
NM_171846
0.005162234
1.522293


002
NM_001943
0.019467462
2.3323212


003
NM_033669
0.01684671
1.7791877


004
NM_133376
0.012459265
1.7374801


005
BC036246
0.002290308
1.5143739


006
BC010850
0.001210456
1.6267678


007
AF495383
0.012324799
2.1980886


008
BC020718
0.007528743
2.5875902


009
AY166714
0.004961676
1.8687251


010
BC042832
0.010989661
1.7944587


011
BE708432
0.00670648
1.6990829


012
Z48481
0.005463324
1.5193439


013
M18468
0.017312625
1.5803499


014
AL567295
0.007407852
1.6839108


015
NM_004663
0.000163535
1.5653288


016
NM_006801
0.004791257
1.638207


017
NM_003405
0.009279575
1.5148095


018
J03005
0.014834337
1.5069977


019
U36189
0.011555359
2.1326842


020
AB020657
0.00119686
1.5838884


021
NM_002291
0.000568398
1.8073287


022
NM_000358
0.008768089
1.9059453


023
W02593
0.010550437
1.5276276


024
AF109196
0.002245945
2.1570368


025
NM_003088
0.007795681
2.1724482


026
NM_005397
0.00303411
1.8943018


027
NM_004342
0.01926877
1.8294148


028
AA885297
0.006063032
1.6768507


029
NM_005506
0.015885489
1.6841809


030
NM_000270
0.018524641
1.6833633


031
AV692127
0.009770202
1.5369248


032
AI927944
0.00259865
1.6228764


033
D85390
0.017363481
1.7431495


034
NM_005562
0.004330024
2.8191426


035
BC005359
0.008048828
1.5254242


036
NM_001110
0.002003783
1.767903


037
AL574210
0.00091248
3.0523725


038
NM_000602
0.00504642
2.6835847


039
NM_005637
0.005462693
1.5148987


040
NM_000584
0.014948112
2.1844351


041
NM_000346
0.019816045
2.2876046


042
NM_001450
0.006776552
2.2249734


043
NM_002318
0.006687925
2.0231075


044
NM_014863
0.00419499
1.5032523


045
NM_004998
0.000449373
1.5877136


046
NM_005570
0.002661762
1.9762497


047
U09716
0.000473367
1.9764429


048
NM_012329
0.001585437
1.6128623


049
NM_006575
0.010453912
1.5251595


050
NM_000846
0.004046575
4.2017674


051
NM_004480
0.01139016
1.6090198


052
NM_006815
0.015985437
1.6165011


053
NM_001511
0.001788037
3.218731


054
NM_004289
0.015985157
1.7023398


055
NM_005904
0.013379821
1.7179344


056
NM_002841
0.004963213
1.769544


057
BF305661
0.012746719
2.1320713


058
U29586
0.013908542
1.7317705


059
NM_001109
0.000473816
2.054043


060
NM_002658
0.003415823
2.4370956


061
AW188198
0.013965369
2.1515768


062
NM_004162
0.010821017
1.571063


063
NM_000366
0.000283653
2.0841253


064
NM_006469
0.003607815
1.5105128


065
NM_002547
0.018292218
1.5056778


066
NM_015869
0.002361554
1.882336


067
AV712733
0.001033398
1.7958127


068
BC003576
0.000448714
1.631627


069
AF263279
0.017487219
1.5380286


070
L18887
0.011792572
1.5100785


071
U55936
0.001730693
1.8878508


072
AW469573
0.009551367
1.9820172


073
AK024258
0.00507411
1.6641864


074
BC001465
0.006051209
1.6464524


075
D86962
0.01098607
1.7481923


076
AF000017
0.013879589
1.701537


077
AF000416
0.003979554
1.5687809


078
U08839
0.007479298
1.7924315


079
BC004472
0.003141172
1.619537


080
BC004908
0.00342191
1.906748


081
M19267
0.004614187
1.6935222


082
BC003683
0.015057402
1.5387125


083
AF043337
0.005428782
2.867063


084
L49506
0.010491861
1.8367761


085
U19348
0.019789577
1.9247686


086
M20206
0.000418344
1.997547


087
K03226
0.00240352
2.8568754


088
AF207990
0.011889962
1.7860718


089
AY029180
0.011789334
1.8189595


090
NM_001845
0.007531395
1.8490748


091
BF342851
0.016265145
1.8463359


092
AI735639
0.007391165
1.5595657


093
AI670847
0.016607396
1.5904158


094
AI972416
0.002460855
1.63999


095
AI761250
0.010427832
1.6232696


096
BF739959
0.003050241
1.8505166


097
AA873600
0.005912889
1.8562527


098
AW241752
0.006011819
1.9170463


099
AA086229
5.50514E−05
1.5048952


100
AA602532
0.015398935
1.5939685


101
NM_003825
0.003539713
1.8040004


102
BE568134
0.002274355
2.2189345


103
AJ276395
0.001182322
2.071262


104
X74039
0.003173471
1.7340106


105
AU135154
0.004170008
1.9890832


106
AK022513
0.018290011
1.5388945


107
AV721430
0.000657631
1.7091621


108
AJ270770
0.004103699
1.5264177


109
S69182
0.005493577
1.6935816


110
Z54367
0.01826363
1.7186335


111
AC007182
0.011925477
1.6185476


112
AL117508
0.007782524
1.5433311


113
AF023139
0.007220107
1.5624946


114
NM_007350
0.016502094
1.6960312


115
BE542551
0.01684034
1.5417765


116
NM_016598
0.010970607
1.5836283


117
NM_013238
0.019865552
1.7292447


118
NM_016445
0.000675608
2.7071812


119
NM_006544
0.012352341
1.7368068


120
NM_018022
0.000753902
1.6477742


121
NM_017515
0.009280122
1.6340361


122
NM_013352
0.014419112
1.6285655


123
NM_016629
0.01292243
1.617686


124
NM_024642
0.014052196
1.6402073


125
NM_018004
0.018847797
2.0938365


126
NM_015919
0.005593323
1.5408667


127
NM_022831
0.016323
1.5315142


128
NM_018181
0.001976648
1.5441327


129
NM_030791
0.008873873
1.9432548


130
AI638420
0.004401053
1.7742935


131
BG104571
0.00011337
1.5270268


132
AL035541
0.005303006
2.2757804


133
BG251467
0.014745607
1.738053


134
BG286920
0.005694628
1.5068418


135
BF444916
0.001075083
1.5835624


136
BF444916
0.000622161
1.7766397


137
AA554045
0.003090952
1.8790901


138
AA827878
0.003659447
1.6790042


139
AF132202
0.016841894
1.9524238


140
AI446756
0.012874936
1.6367766


141
AI934753
0.002428954
1.6452742


142
AL574900
0.013543638
1.5199631


143
AA019338
0.013437813
1.5261155


144
AA861416
0.002285053
1.6213596


145
BF438116
0.016804362
1.5368354


146
T78406
0.006987929
1.5712297


147
AB046780
0.000390623
1.7006425


148
AL547782
0.005000754
1.770981


149
BE207755
0.003047524
1.6990312


150
AI888037
0.004693668
1.8490914


151
AK026181
0.014052763
1.8735564


152
AA554833
0.016480966
1.9064581


153
BF447037
0.001219355
1.5196482


154
W63676
0.005363467
1.8277074


155
BF540829
0.001859941
1.5185972


156
AW973842
0.000646104
1.6094143


157
AL571557
0.006222301
2.0978951


158
AI091398
0.01153802
1.7936606


159
AI692645
0.005127667
1.6694399


160
NM_014086
0.004784999
1.72546


161
BG054798
0.002478401
1.5713933


162
BG400596
0.018314553
1.5202585


163
AI697540
0.002645486
2.0540323


164
AI445833
0.004576562
1.805269


165
AV762916
0.012934915
1.6069295


166
BE176566
0.01719282
1.5133282.

2. The method of claim 1, wherein the measuring of an expression level of a target nucleic acid is performed by measuring a level of mRNA or protein encoded by the target nucleic acid.

3. The method of claim 1, wherein expression levels in the lung cancer tissue sample correspond to expression levels of the recurrence group when the expression levels in the lung cancer tissue sample show a statistically meaningful difference from the expression levels in the nonrecurrence group or do not show a statistically meaningful difference from the expression levels in the recurrence group; and
expression levels in the lung cancer tissue sample correspond to expression levels of the nonrecurrence group when the expression levels in the lung cancer tissue sample show a statistically meaningful difference from the expression levels in the recurrence group or do not show a statistically meaningful difference from the expression levels in the nonrecurrence group,
wherein a statistically meaningful difference is expressed as a p value for the difference of less than 0.05.

4. The method of claim 3, wherein determining statistical significance of a difference between expression levels in the lung cancer tissue sample and the recurrence group or the nonrecurrence group is analyzed using a statistical model selected from the group consisting of Linear Discrimination Analysis (LDA) model, a Quadratic Discrimination Analysis (QDA) prediction model, a Neural Network model, a Decision Tree model, a Support Vector Machine model and a Naive Bayes model.

5. The method of claim 1, wherein the expression level is measured on a microarray.

6. The method of claim 5, wherein the microarray is a nucleic acid microarray.

7. The method of claim 1, wherein the expression level of the target nucleic acid is determined by measuring the amount of an amplification product obtained by nucleic acid amplification that is carried out by a reverse transcriptase-polymerase chain reaction (RT-PCR) using RNA as a template.

8. The method of claim 1, wherein the expression level is measured by detecting protein encoded by the target nucleic acid.

9. The method of claim 8, wherein the detecting of protein is performed by using an antibody specific to the protein.

10. A composition for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising at least one probe or probe set specific to each target nucleic acid in a set of target nucleic acids consisting of target nucleic acids in Tables 1, wherein Table 1 is shown below




















Genbank

Fold change


No.
Probe Set ID
Gene Name
Gene Symbol
Accession #
T-test p-value
(abs)





















001
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.005162234
1.522293


002
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.019467462
2.3323212


003
1553530_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_033669
0.01684671
1.7791877




polypeptide,




antigen CD29 includes MDF2, MSK12)


004
1553678_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_133376
0.012459265
1.7374601




polypeptide,




antigen CD29 includes MDF2, MSK12)


005
1554067_at
hypothetical protein FLJ32549
FLJ32549
BC036246
0.002290308
1.5143739


006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397
BC010850
0.001210456
1.6267678


007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin
ADAM9
AF495383
0.012324799
2.1980886




gamma)


008
1555564_a_at
I factor (complement)
IF
BC020718
0.007528743
2.5875902


009
1555705_a_at
chemokine-like factor superfamily 3
CKLFSF3
AY166714
0.004961676
1.8687251


010
1557987_at
PI-3-kinase-related kinase SMG-1 - like locus
LOC641298
BC042832
0.010989661
1.7944587


011
1558678_s_at
metastasis associated lung adenocarcinoma
MALAT1
BE708432
0.00670648
1.6990829




transcript 1




(non-coding RNA)


012
160020_at
matrix metallopeptidase 14 (membrane-
MMP14
Z48481
0.005463324
1.5193439




inserted)


013
200604_s_at
protein kinase, cAMP-dependent, regulatory,
PRKAR1A
M18468
0.017312625
1.5803499




type I, alpha (tissue specific extinguisher 1)


014
200615_s_at
adaptor-related protein complex 2, beta 1
AP2B1
AL567295
0.007407852
1.6839108




subunit


015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.000163535
1.5653288


016
200922_at
KDEL (Lys-Asp-Glu-Leu) endoplasmic
KDELR1
NM_006801
0.004791257
1.638207




reticulum




protein retention receptor 1


017
201020_at
tyrosine 3-monooxygenase/tryptophan 5-
YWHAH
NM_003405
0.009279575
1.5148095




monooxygenase




activation protein, eta polypeptide


018
201179_s_at
guanine nucleotide binding protein (G protein),
GNAI3
J03005
0.014834337
1.5069977




alpha inhibiting activity polypeptide 3


019
201309_x_at
chromosome 5 open reading frame 13
C5orf13
U36189
0.011555359
2.1326842


020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.00119686
1.5838884


021
201505_at
laminin, beta 1
LAMB1
NM_002291
0.000568398
1.8073287


022
201506_at
transforming growth factor, beta-induced,
TGFBI
NM_000358
0.008768089
1.9059453




68 kDa


023
201548_s_at
Jumonji, AT rich interactive domain 1B
JARID1B
W02593
0.010550437
1.5276276




(RBP2-like)


024
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.002245945
2.1570368


025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.007795681
2.1724482




(Strongylocentrotus purpuratus)


026
201578_at
podocalyxin-like
PODXL
NM_005397
0.00303411
1.8943018


027
201617_x_at
caldesmon 1
CALD1
NM_004342
0.01926877
1.8294148


028
201646_at
scavenger receptor class B, member 2
SCARB2 
AA885297
0.006063032
1.6768507


029
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.015885489
1.6841809


030
201695_s_at
nucleoside phosphorylase
NP
NM_000270
0.018524641
1.6833633


031
201722_s_at
UDP-N-acetyl-alpha-D-
GALNT1
AV692127
0.009770202
1.5369248




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 1




(GalNAc-T1)


032
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.00259865
1.6228764


033
201942_s_at
carboxypeptidase D
CPD
D85390
0.017363481
1.7431495


034
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.004330024
2.8191426


035
202543_s_at
glia maturation factor, beta
GMFB
BC005359
0.008048828
1.5254242


036
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.002003783
1.767903


037
202627_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
AL574210
0.00091248
3.0523725




plasminogen activator inhibitor type 1),




member 1


038
202628_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
NM_000602
0.00504642
2.6835847




plasminogen activator inhibitor type 1),




member 1


039
202817_s_at
synovial sarcoma translocation, chromosome
SS18
NM_005637
0.005462693
1.5148987




18


040
202859_x_at
interleukin 8
IL8
NM_000584
0.014948112
2.1844351


041
202936_s_at
SRY (sex determining region Y)-box 9
SOX9
NM_000346
0.019816045
2.2876046




(campomelic dysplasia, autosomal sex-




reversal


042
202949_s_at
four and a half LIM domains 2
FHL2
NM_001450
0.006776552
2.2249734


043
202998_s_at
lysyl oxidase-like 2
LOXL2
NM_002318
0.006687925
2.0231075


044
203066_at
B cell RAG associated protein
GALNAC4S-
NM_014863
0.00419499
1.5032523





6ST


045
203072_at
myosin IE
MYO1E
NM_004998
0.000449373
1.5877136


046
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.002661762
1.9762497


047
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.000473367
1.9764429


048
203414_at
monocyte to macrophage differentiation-
MMD
NM_012329
0.001585437
1.6128623




associated


049
203553_s_at
mitogen-activated protein kinase kinase kinase
MAP4K5
NM_006575
0.010453912
1.5251595




kinase 5


050
203924_at
glutathione S-transferase A1
GSTA1
NM_000846
0.004046575
4.2017674


051
203988_s_at
fucosyltransferase 8 (alpha (1,6)
FUT8
NM_004480
0.01139016
1.6090198




fucosyltransferase)


052
204426_at
transmembrane emp24 domain trafficking
TMED2
NM_006815
0.015985437
1.6165011




protein 2


053
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.001788037
3.218731




(melanoma growth stimulating activity, alpha)


054
204702_s_at
nuclear factor (erythroid-derived 2)-like 3
NFE2L3
NM_004289
0.015985157
1.7023398


055
204790_at
SMAD, mothers against DPP homolog 7
SMAD7
NM_005904
0.013379821
1.7179344




(Drosophila)


056
204944_at
protein tyrosine phosphatase, receptor type, G
PTPRG
NM_002841
0.004963213
1.769544


057
204989_s_at
integrin, beta 4
ITGB4
BF305661
0.012746719
2.1320713


058
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-
SGCB
U29586
0.013908542
1.7317705




associated glycoprotein)


059
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.000473816
2.054043


060
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.003415823
2.4370956


061
206025_s_at
tumor necrosis factor, alpha-induced protein 6
TNFAIP6
AW188198
0.013965369
2.1515768


062
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.010821017
1.571063


063
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.000283653
2.0841253


064
206245_s_at
influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.003607815
1.5105128


065
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.018292218
1.5056778


066
208510_s_at
peroxisome proliferative activated receptor,
PPARG
NM_015869
0.002361554
1.882336




gamma


067
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.001033398
1.7958127


068
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.000448714
1.631627


069
208653_s_at
CD164 antigen, sialomucin
CD164
AF263279
0.017487219
1.5380286


070
208853_s_at
calnexin
CANX
L18887
0.011792572
1.5100785


071
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.001730693
1.8878508


072
209209_s_at
pleckstrin homology domain containing,
PLEKHC1
AW469573
0.009551367
1.9820172




family C




(with FERM domain) member 1


073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.00507411
1.6641864


074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L
BC001465
0.006051209
1.6464524


075
209409_at
growth factor receptor-bound protein 10
GRB10
D86962
0.01098607
1.7481923


076
209410_s_at
growth factor receptor-bound protein 10
GRB10
AF000017
0.013879589
1.701537


077
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.003979554
1.5687809


078
210845_s_at
plasminogen activator, urokinase receptor
PLAUR
U08839
0.007479298
1.7924315


079
210892_s_at
general transcription factor II,i
GTF2I
BC004472
0.003141172
1.619537


080
210933_s_at
fascin homolog 1, actin-bundling protein
FSCN1
BC004908
0.00342191
1.906748




(Strongylocentrotus purpuratus)


081
210987_x_at
tropomyosin 1 (alpha)
TPM1
M19267
0.004614187
1.6935222


082
211299_s_at
flotillin 2
FLOT2
BC003683
0.015057402
1.5387125


083
211506_s_at
interleukin 8
IL8
AF043337
0.005428782
2.867063


084
211559_s_at
cyclin G2
CCNG2
L49506
0.010491861
1.8367761


085
211599_x_at
met proto-oncogene (hepatocyte growth factor
MET
U19348
0.019789577
1.9247686




receptor)


086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.000418344
1.997547


087
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.00240352
2.8568754


088
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.011889962
1.7860718


089
211924_s_at
plasminogen activator, urokinase receptor
PLAUR
AY029180
0.011789334
1.8189595


090
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.007531395
1.8490748


091
212012_at
peroxidasin homolog (Drosophila)
PXDN
BF342851
0.016265145
1.8463359


092
212660_at
PHD finger protein 15
PHF15
AI735639
0.007391165
1.5595657


093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.016607396
1.5904158


094
212907_at
Solute carrier family 30 (zinc transporter),
SLC30A1
AI972416
0.002460855
1.63999




member 1


095
213288_at
O-acyltransferase (membrane bound) domain
OACT2
AI761250
0.010427832
1.6232696




containing 2


096
213457_at
malignant fibrous histiocytoma amplified
MFHAS1
BF739959
0.003050241
1.8505166




sequence 1


097
213624_at
sphingomyelin phosphodiesterase, acid-like 3A
SMPDL3A
AA873600
0.005912889
1.8562527


098
213742_at
splicing factor, arginine/serine-rich 11
SFRS11
AW241752
0.006011819
1.9170463


099
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
5.50514E−05
1.5048952


100
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.015398935
1.5939685


101
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.003539713
1.8040004


102
214581_x_at
tumor necrosis factor receptor superfamily,
TNFRSF21
BE568134
0.002274355
2.2189345




member 21


103
214701_s_at
fibronectin 1
FN1
AJ276395
0.001182322
2.071262


104
214866_at
plasminogen activator, urokinase receptor
PLAUR
X74039
0.003173471
1.7340106


105
214895_s_at
ADAM metallopeptidase domain 10
ADAM10
AU135154
0.004170008
1.9890832


106
215501_s_at
dual specificity phosphatase 10
DUSP10
AK022513
0.018290011
1.5388945


107
216035_x_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AV721430
0.000657631
1.7091621




HMG-box)


108
216511_s_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AJ270770
0.004103699
1.5264177




HMG-box)


109
216915_s_at
protein tyrosine phosphatase, non-receptor type
PTPN12
S69182
0.005493577
1.6935816




12


110
216971_s_at
plectin 1, intermediate filament binding protein
PLEC1
Z54367
0.01826363
1.7186335




500 kDa


111
217188_s_at
chromosome 14 open reading frame 1
C14orf1
AC007182
0.011925477
1.6185476


112
217448_s_at
chromosome 14 open reading frame 92
C14orf92
AL117508
0.007782524
1.5433311




similar to Epidermal Langerhans cell protein
LOC285412




LCP1


113
217492_s_at
phosphatase and tensin homolog
PTEN
AF023139
0.007220107
1.5624946




(mutated in multiple advanced cancers 1)


114
218000_s_at
pleckstrin homology-like domain, family A,
PHLDA1
NM_007350
0.016502094
1.6960312




member 1


115
218077_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
BE542551
0.01684034
1.5417765


116
218078_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
NM_016598
0.010970607
1.5836283


117
218435_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
NM_013238
0.019865552
1.7292447




15


118
218644_at
pleckstrin 2
PLEK2
NM_016445
0.000675608
2.7071812


119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1
NM_006544
0.012352341
1.7368068


120
218815_s_at
transmembrane protein 51
TMEM51
NM_018022
0.000753902
1.6477742


121
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.009280122
1.6340361


122
218854_at
squamous cell carcinoma
SART2
NM_013352
0.014419112
1.6285655




antigen recognized by T cells 2


123
218856_at
tumor necrosis factor receptor superfamily,
TNFRSF21
NM_016629
0.01292243
1.617686




member 21


124
218885_s_at
UDP-N-acetyl-alpha-D-
GALNT12
NM_024642
0.014052196
1.6402073




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


125
219410_at
transmembrane protein 45A
TMEM45A
NM_018004
0.018847797
2.0938365


126
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.005593323
1.5408667


127
220199_s_at
chromosome 1 open reading frame 80
C1orf80
NM_022831
0.016323
1.5315142


128
220617_s_at
zinc finger protein 532
ZNF532
NM_018181
0.001976648
1.5441327


129
221268_s_at
sphingosine-1-phosphate phosphatase 1
SGPP1
NM_030791
0.008873873
1.9432548


130
221881_s_at
chloride intracellular channel 4
CLIC4
AI638420
0.004401053
1.7742935


131
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.00011337
1.5270268


132
222449_at
transmembrane, prostate
TMEPAI
AL035541
0.005303006
2.2757804




androgen induced RNA


133
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.014745607
1.738053


134
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.005694628
1.5068418


135
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.001075083
1.5835624


136
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.000622161
1.7766397


137
222773_s_at
UDP-N-acetyl-alpha-D-
GALNT12
AA554045
0.003090952
1.8790901




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


138
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.003659447
1.6790042


139
223940_x_at
metastasis associated lung adenocarcinoma
MALAT1
AF132202
0.016841894
1.9524238




transcript 1 (non-coding RNA)


140
224558_s_at
metastasis associated lung adenocarcinoma
MALAT1
AI446756
0.012874936
1.6367766




transcript 1 (non-coding RNA)


141
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.002428954
1.6452742


142
224733_at
chemokine-like factor superfamily 3
CKLFSF3
AL574900
0.013543638
1.5199631


143
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.013437813
1.5261155


144
225021_at
zinc finger protein 532
ZNF532
AA861416
0.002285053
1.6213596


145
225140_at
Kruppel-like factor 3 (basic)
KLF3
BF438116
0.016804362
1.5368354


146
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.006987929
1.5712297


147
225424_at
glycerol-3-phosphate acyltransferase,
GPAM
AB046780
0.000390623
1.7006425




mitochondrial


148
225503_at
dehydrogenase/reductase (SDR family)
DHRSX
AL547782
0.005000754
1.770981




X-linked


149
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.003047524
1.6990312


150
225609_at
glutathione reductase
GSR
AI888037
0.004693668
1.8490914


151
225842_at
Pleckstrin homology-like domain, family A,
PHLDA1
AK026181
0.014052763
1.8735564




member 1


152
226084_at
microtubule-associated protein 1B
MAP1B
AA554833
0.016480966
1.9064581


153
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.001219355
1.5196482


154
226726_at
O-acyltransferase (membrane bound) domain
OACT2
W63676
0.005363467
1.8277074




containing 2


155
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.001859941
1.5185972


156
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.000646104
1.6094143


157
227628_at
similar to RIKEN cDNA 2310016C16
LOC493869
AL571557
0.006222301
2.0978951


158
227808_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
AI091398
0.01153802
1.7936606




15


159
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.005127667
1.6694399


160
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.004784999
1.72546


161
231823_s_at
KIAA1295
KIAA1295
BG054798
0.002478401
1.5713933


162
235587_at
hypothetical protein LOC202781
LOC202781
BG400596
0.018314553
1.5202585


163
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.002645486
2.0540323


164
238558_at
Muscleblind-like (Drosophila)
MBNL1
AI445833
0.004576562
1.805269


165
238563_at
Ab1-interactor 1
ABI1
AV762916
0.012934915
1.6069295


166
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01719282
1.5133282.

11. A kit for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, the kit comprising at least one probe or probe set specific to each target nucleic acid in a set of target nucleic acids consisting of target nucleic acids in Tables 1, below




















Genbank

Fold change


No.
Probe Set ID
Gene Name
Gene Symbol
Accession #
T-test p-value
(abs)





















001
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.005162234
1.522293


002
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.019467462
2.3323212


003
1553530_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_033669
0.01684671
1.7791877




polypeptide,




antigen CD29 includes MDF2, MSK12)


004
1553678_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_133376
0.012459265
1.7374601




polypeptide,




antigen CD29 includes MDF2, MSK12)


005
1554067_at
hypothetical protein FLJ32549
FLJ32549
BC036246
0.002290308
1.5143739


006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397
BC010850
0.001210456
1.6267678


007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin
ADAM9
AF495383
0.012324799
2.1980886




gamma)


008
1555564_a_at
I factor (complement)
IF
BC020718
0.007528743
2.5875902


009
1555705_a_at
chemokine-like factor superfamily 3
CKLFSF3
AY166714
0.004961676
1.8687251


010
1557987_at
PI-3-kinase-related kinase SMG-1 - like locus
LOC641298
BC042832
0.010989661
1.7944587


011
1558678_s_at
metastasis associated lung adenocarcinoma
MALAT1
BE708432
0.00670648
1.6990829




transcript 1




(non-coding RNA)


012
160020_at
matrix metallopeptidase 14 (membrane-
MMP14
Z48481
0.005463324
1.5193439




inserted)


013
200604_s_at
protein kinase, cAMP-dependent, regulatory,
PRKAR1A
M18468
0.017312625
1.5803499




type I, alpha (tissue specific extinguisher 1)


014
200615_s_at
adaptor-related protein complex 2, beta 1
AP2B1
AL567295
0.007407852
1.6839108




subunit


015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.000163535
1.5653288


016
200922_at
KDEL (Lys-Asp-Glu-Leu) endoplasmic
KDELR1
NM_006801
0.004791257
1.638207




reticulum




protein retention receptor 1


017
201020_at
tyrosine 3-monooxygenase/tryptophan 5-
YWHAH
NM_003405
0.009279575
1.5148095




monooxygenase




activation protein, eta polypeptide


018
201179_s_at
guanine nucleotide binding protein (G protein),
GNAI3
J03005
0.014834337
1.5069977




alpha inhibiting activity polypeptide 3


019
201309_x_at
chromosome 5 open reading frame 13
C5orf13
U36189
0.011555359
2.1326842


020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.00119686
1.5838884


021
201505_at
laminin, beta 1
LAMB1
NM_002291
0.000568398
1.8073287


022
201506_at
transforming growth factor, beta-induced,
TGFBI
NM_000358
0.008768089
1.9059453




68 kDa


023
201548_s_at
Jumonji, AT rich interactive domain 1B
JARID1B
W02593
0.010550437
1.5276276




(RBP2-like)


024
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.002245945
2.1570368


025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.007795681
2.1724482




(Strongylocentrotus purpuratus)


026
201578_at
podocalyxin-like
PODXL
NM_005397
0.00303411
1.8943018


027
201617_x_at
caldesmon 1
CALD1
NM_004342
0.01926877
1.8294148


028
201646_at
scavenger receptor class B, member 2
SCARB2 
AA885297
0.006063032
1.6768507


029
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.015885489
1.6841809


030
201695_s_at
nucleoside phosphorylase
NP
NM_000270
0.018524641
1.6833633


031
201722_s_at
UDP-N-acetyl-alpha-D-
GALNT1
AV692127
0.009770202
1.5369248




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 1




(GalNAc-T1)


032
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.00259865
1.6228764


033
201942_s_at
carboxypeptidase D
CPD
D85390
0.017363481
1.7431495


034
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.004330024
2.8191426


035
202543_s_at
glia maturation factor, beta
GMFB
BC005359
0.008048828
1.5254242


036
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.002003783
1.767903


037
202627_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
AL574210
0.00091248
3.0523725




plasminogen activator inhibitor type 1),




member 1


038
202628_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
NM_000602
0.00504642
2.6835847




plasminogen activator inhibitor type 1),




member 1


039
202817_s_at
synovial sarcoma translocation, chromosome
SS18
NM_005637
0.005462693
1.5148987




18


040
202859_x_at
interleukin 8
IL8
NM_000584
0.014948112
2.1844351


041
202936_s_at
SRY (sex determining region Y)-box 9
SOX9
NM_000346
0.019816045
2.2876046




(campomelic dysplasia, autosomal sex-




reversal


042
202949_s_at
four and a half LIM domains 2
FHL2
NM_001450
0.006776552
2.2249734


043
202998_s_at
lysyl oxidase-like 2
LOXL2
NM_002318
0.006687925
2.0231075


044
203066_at
B cell RAG associated protein
GALNAC4S-
NM_014863
0.00419499
1.5032523





6ST


045
203072_at
myosin IE
MYO1E
NM_004998
0.000449373
1.5877136


046
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.002661762
1.9762497


047
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.000473367
1.9764429


048
203414_at
monocyte to macrophage differentiation-
MMD
NM_012329
0.001585437
1.6128623




associated


049
203553_s_at
mitogen-activated protein kinase kinase kinase
MAP4K5
NM_006575
0.010453912
1.5251595




kinase 5


050
203924_at
glutathione S-transferase A1
GSTA1
NM_000846
0.004046575
4.2017674


051
203988_s_at
fucosyltransferase 8 (alpha (1,6)
FUT8
NM_004480
0.01139016
1.6090198




fucosyltransferase)


052
204426_at
transmembrane emp24 domain trafficking
TMED2
NM_006815
0.015985437
1.6165011




protein 2


053
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.001788037
3.218731




(melanoma growth stimulating activity, alpha)


054
204702_s_at
nuclear factor (erythroid-derived 2)-like 3
NFE2L3
NM_004289
0.015985157
1.7023398


055
204790_at
SMAD, mothers against DPP homolog 7
SMAD7
NM_005904
0.013379821
1.7179344




(Drosophila)


056
204944_at
protein tyrosine phosphatase, receptor type, G
PTPRG
NM_002841
0.004963213
1.769544


057
204989_s_at
integrin, beta 4
ITGB4
BF305661
0.012746719
2.1320713


058
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-
SGCB
U29586
0.013908542
1.7317705




associated glycoprotein)


059
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.000473816
2.054043


060
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.003415823
2.4370956


061
206025_s_at
tumor necrosis factor, alpha-induced protein 6
TNFAIP6
AW188198
0.013965369
2.1515768


062
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.010821017
1.571063


063
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.000283653
2.0841253


064
206245_s_at
influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.003607815
1.5105128


065
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.018292218
1.5056778


066
208510_s_at
peroxisome proliferative activated receptor,
PPARG
NM_015869
0.002361554
1.882336




gamma


067
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.001033398
1.7958127


068
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.000448714
1.631627


069
208653_s_at
CD164 antigen, sialomucin
CD164
AF263279
0.017487219
1.5380286


070
208853_s_at
calnexin
CANX
L18887
0.011792572
1.5100785


071
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.001730693
1.8878508


072
209209_s_at
pleckstrin homology domain containing,
PLEKHC1
AW469573
0.009551367
1.9820172




family C




(with FERM domain) member 1


073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.00507411
1.6641864


074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L
BC001465
0.006051209
1.6464524


075
209409_at
growth factor receptor-bound protein 10
GRB10
D86962
0.01098607
1.7481923


076
209410_s_at
growth factor receptor-bound protein 10
GRB10
AF000017
0.013879589
1.701537


077
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.003979554
1.5687809


078
210845_s_at
plasminogen activator, urokinase receptor
PLAUR
U08839
0.007479298
1.7924315


079
210892_s_at
general transcription factor II,i
GTF2I
BC004472
0.003141172
1.619537


080
210933_s_at
fascin homolog 1, actin-bundling protein
FSCN1
BC004908
0.00342191
1.906748




(Strongylocentrotus purpuratus)


081
210987_x_at
tropomyosin 1 (alpha)
TPM1
M19267
0.004614187
1.6935222


082
211299_s_at
flotillin 2
FLOT2
BC003683
0.015057402
1.5387125


083
211506_s_at
interleukin 8
IL8
AF043337
0.005428782
2.867063


084
211559_s_at
cyclin G2
CCNG2
L49506
0.010491861
1.8367761


085
211599_x_at
met proto-oncogene (hepatocyte growth factor
MET
U19348
0.019789577
1.9247686




receptor)


086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.000418344
1.997547


087
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.00240352
2.8568754


088
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.011889962
1.7860718


089
211924_s_at
plasminogen activator, urokinase receptor
PLAUR
AY029180
0.011789334
1.8189595


090
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.007531395
1.8490748


091
212012_at
peroxidasin homolog (Drosophila)
PXDN
BF342851
0.016265145
1.8463359


092
212660_at
PHD finger protein 15
PHF15
AI735639
0.007391165
1.5595657


093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.016607396
1.5904158


094
212907_at
Solute carrier family 30 (zinc transporter),
SLC30A1
AI972416
0.002460855
1.63999




member 1


095
213288_at
O-acyltransferase (membrane bound) domain
OACT2
AI761250
0.010427832
1.6232696




containing 2


096
213457_at
malignant fibrous histiocytoma amplified
MFHAS1
BF739959
0.003050241
1.8505166




sequence 1


097
213624_at
sphingomyelin phosphodiesterase, acid-like 3A
SMPDL3A
AA873600
0.005912889
1.8562527


098
213742_at
splicing factor, arginine/serine-rich 11
SFRS11
AW241752
0.006011819
1.9170463


099
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
5.50514E−05
1.5048952


100
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.015398935
1.5939685


101
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.003539713
1.8040004


102
214581_x_at
tumor necrosis factor receptor superfamily,
TNFRSF21
BE568134
0.002274355
2.2189345




member 21


103
214701_s_at
fibronectin 1
FN1
AJ276395
0.001182322
2.071262


104
214866_at
plasminogen activator, urokinase receptor
PLAUR
X74039
0.003173471
1.7340106


105
214895_s_at
ADAM metallopeptidase domain 10
ADAM10
AU135154
0.004170008
1.9890832


106
215501_s_at
dual specificity phosphatase 10
DUSP10
AK022513
0.018290011
1.5388945


107
216035_x_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AV721430
0.000657631
1.7091621




HMG-box)


108
216511_s_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AJ270770
0.004103699
1.5264177




HMG-box)


109
216915_s_at
protein tyrosine phosphatase, non-receptor type
PTPN12
S69182
0.005493577
1.6935816




12


110
216971_s_at
plectin 1, intermediate filament binding protein
PLEC1
Z54367
0.01826363
1.7186335




500 kDa


111
217188_s_at
chromosome 14 open reading frame 1
C14orf1
AC007182
0.011925477
1.6185476


112
217448_s_at
chromosome 14 open reading frame 92
C14orf92
AL117508
0.007782524
1.5433311




similar to Epidermal Langerhans cell protein
LOC285412




LCP1


113
217492_s_at
phosphatase and tensin homolog
PTEN
AF023139
0.007220107
1.5624946




(mutated in multiple advanced cancers 1)


114
218000_s_at
pleckstrin homology-like domain, family A,
PHLDA1
NM_007350
0.016502094
1.6960312




member 1


115
218077_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
BE542551
0.01684034
1.5417765


116
218078_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
NM_016598
0.010970607
1.5836283


117
218435_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
NM_013238
0.019865552
1.7292447




15


118
218644_at
pleckstrin 2
PLEK2
NM_016445
0.000675608
2.7071812


119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1
NM_006544
0.012352341
1.7368068


120
218815_s_at
transmembrane protein 51
TMEM51
NM_018022
0.000753902
1.6477742


121
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.009280122
1.6340361


122
218854_at
squamous cell carcinoma
SART2
NM_013352
0.014419112
1.6285655




antigen recognized by T cells 2


123
218856_at
tumor necrosis factor receptor superfamily,
TNFRSF21
NM_016629
0.01292243
1.617686




member 21


124
218885_s_at
UDP-N-acetyl-alpha-D-
GALNT12
NM_024642
0.014052196
1.6402073




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


125
219410_at
transmembrane protein 45A
TMEM45A
NM_018004
0.018847797
2.0938365


126
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.005593323
1.5408667


127
220199_s_at
chromosome 1 open reading frame 80
C1orf80
NM_022831
0.016323
1.5315142


128
220617_s_at
zinc finger protein 532
ZNF532
NM_018181
0.001976648
1.5441327


129
221268_s_at
sphingosine-1-phosphate phosphatase 1
SGPP1
NM_030791
0.008873873
1.9432548


130
221881_s_at
chloride intracellular channel 4
CLIC4
AI638420
0.004401053
1.7742935


131
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.00011337
1.5270268


132
222449_at
transmembrane, prostate
TMEPAI
AL035541
0.005303006
2.2757804




androgen induced RNA


133
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.014745607
1.738053


134
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.005694628
1.5068418


135
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.001075083
1.5835624


136
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.000622161
1.7766397


137
222773_s_at
UDP-N-acetyl-alpha-D-
GALNT12
AA554045
0.003090952
1.8790901




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


138
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.003659447
1.6790042


139
223940_x_at
metastasis associated lung adenocarcinoma
MALAT1
AF132202
0.016841894
1.9524238




transcript 1 (non-coding RNA)


140
224558_s_at
metastasis associated lung adenocarcinoma
MALAT1
AI446756
0.012874936
1.6367766




transcript 1 (non-coding RNA)


141
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.002428954
1.6452742


142
224733_at
chemokine-like factor superfamily 3
CKLFSF3
AL574900
0.013543638
1.5199631


143
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.013437813
1.5261155


144
225021_at
zinc finger protein 532
ZNF532
AA861416
0.002285053
1.6213596


145
225140_at
Kruppel-like factor 3 (basic)
KLF3
BF438116
0.016804362
1.5368354


146
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.006987929
1.5712297


147
225424_at
glycerol-3-phosphate acyltransferase,
GPAM
AB046780
0.000390623
1.7006425




mitochondrial


148
225503_at
dehydrogenase/reductase (SDR family)
DHRSX
AL547782
0.005000754
1.770981




X-linked


149
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.003047524
1.6990312


150
225609_at
glutathione reductase
GSR
AI888037
0.004693668
1.8490914


151
225842_at
Pleckstrin homology-like domain, family A,
PHLDA1
AK026181
0.014052763
1.8735564




member 1


152
226084_at
microtubule-associated protein 1B
MAP1B
AA554833
0.016480966
1.9064581


153
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.001219355
1.5196482


154
226726_at
O-acyltransferase (membrane bound) domain
OACT2
W63676
0.005363467
1.8277074




containing 2


155
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.001859941
1.5185972


156
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.000646104
1.6094143


157
227628_at
similar to RIKEN cDNA 2310016C16
LOC493869
AL571557
0.006222301
2.0978951


158
227808_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
AI091398
0.01153802
1.7936606




15


159
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.005127667
1.6694399


160
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.004784999
1.72546


161
231823_s_at
KIAA1295
KIAA1295
BG054798
0.002478401
1.5713933


162
235587_at
hypothetical protein LOC202781
LOC202781
BG400596
0.018314553
1.5202585


163
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.002645486
2.0540323


164
238558_at
Muscleblind-like (Drosophila)
MBNL1
AI445833
0.004576562
1.805269


165
238563_at
Ab1-interactor 1
ABI1
AV762916
0.012934915
1.6069295


166
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01719282
1.5133282.

12. The kit of claim 11, wherein the probe or probe set is immobilized on a microarray.

13. A kit for diagnosing the risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, comprising a sense and anti-sense primer pair for each target nucleic acid in a set of target nucleic acids consisting of target nucleic acids in Tables 1, below




















Genbank

Fold change


No.
Probe Set ID
Gene Name
Gene Symbol
Accession #
T-test p-value
(abs)





















001
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.005162234
1.522293


002
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.019467462
2.3323212


003
1553530_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_033669
0.01684671
1.7791877




polypeptide,




antigen CD29 includes MDF2, MSK12)


004
1553678_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_133376
0.012459265
1.7374601




polypeptide,




antigen CD29 includes MDF2, MSK12)


005
1554067_at
hypothetical protein FLJ32549
FLJ32549
BC036246
0.002290308
1.5143739


006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397
BC010850
0.001210456
1.6267678


007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin
ADAM9
AF495383
0.012324799
2.1980886




gamma)


008
1555564_a_at
I factor (complement)
IF
BC020718
0.007528743
2.5875902


009
1555705_a_at
chemokine-like factor superfamily 3
CKLFSF3
AY166714
0.004961676
1.8687251


010
1557987_at
PI-3-kinase-related kinase SMG-1 - like locus
LOC641298
BC042832
0.010989661
1.7944587


011
1558678_s_at
metastasis associated lung adenocarcinoma
MALAT1
BE708432
0.00670648
1.6990829




transcript 1




(non-coding RNA)


012
160020_at
matrix metallopeptidase 14 (membrane-
MMP14
Z48481
0.005463324
1.5193439




inserted)


013
200604_s_at
protein kinase, cAMP-dependent, regulatory,
PRKAR1A
M18468
0.017312625
1.5803499




type I, alpha (tissue specific extinguisher 1)


014
200615_s_at
adaptor-related protein complex 2, beta 1
AP2B1
AL567295
0.007407852
1.6839108




subunit


015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.000163535
1.5653288


016
200922_at
KDEL (Lys-Asp-Glu-Leu) endoplasmic
KDELR1
NM_006801
0.004791257
1.638207




reticulum




protein retention receptor 1


017
201020_at
tyrosine 3-monooxygenase/tryptophan 5-
YWHAH
NM_003405
0.009279575
1.5148095




monooxygenase




activation protein, eta polypeptide


018
201179_s_at
guanine nucleotide binding protein (G protein),
GNAI3
J03005
0.014834337
1.5069977




alpha inhibiting activity polypeptide 3


019
201309_x_at
chromosome 5 open reading frame 13
C5orf13
U36189
0.011555359
2.1326842


020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.00119686
1.5838884


021
201505_at
laminin, beta 1
LAMB1
NM_002291
0.000568398
1.8073287


022
201506_at
transforming growth factor, beta-induced,
TGFBI
NM_000358
0.008768089
1.9059453




68 kDa


023
201548_s_at
Jumonji, AT rich interactive domain 1B
JARID1B
W02593
0.010550437
1.5276276




(RBP2-like)


024
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.002245945
2.1570368


025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.007795681
2.1724482




(Strongylocentrotus purpuratus)


026
201578_at
podocalyxin-like
PODXL
NM_005397
0.00303411
1.8943018


027
201617_x_at
caldesmon 1
CALD1
NM_004342
0.01926877
1.8294148


028
201646_at
scavenger receptor class B, member 2
SCARB2 
AA885297
0.006063032
1.6768507


029
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.015885489
1.6841809


030
201695_s_at
nucleoside phosphorylase
NP
NM_000270
0.018524641
1.6833633


031
201722_s_at
UDP-N-acetyl-alpha-D-
GALNT1
AV692127
0.009770202
1.5369248




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 1




(GalNAc-T1)


032
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.00259865
1.6228764


033
201942_s_at
carboxypeptidase D
CPD
D85390
0.017363481
1.7431495


034
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.004330024
2.8191426


035
202543_s_at
glia maturation factor, beta
GMFB
BC005359
0.008048828
1.5254242


036
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.002003783
1.767903


037
202627_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
AL574210
0.00091248
3.0523725




plasminogen activator inhibitor type 1),




member 1


038
202628_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
NM_000602
0.00504642
2.6835847




plasminogen activator inhibitor type 1),




member 1


039
202817_s_at
synovial sarcoma translocation, chromosome
SS18
NM_005637
0.005462693
1.5148987




18


040
202859_x_at
interleukin 8
IL8
NM_000584
0.014948112
2.1844351


041
202936_s_at
SRY (sex determining region Y)-box 9
SOX9
NM_000346
0.019816045
2.2876046




(campomelic dysplasia, autosomal sex-




reversal


042
202949_s_at
four and a half LIM domains 2
FHL2
NM_001450
0.006776552
2.2249734


043
202998_s_at
lysyl oxidase-like 2
LOXL2
NM_002318
0.006687925
2.0231075


044
203066_at
B cell RAG associated protein
GALNAC4S-
NM_014863
0.00419499
1.5032523





6ST


045
203072_at
myosin IE
MYO1E
NM_004998
0.000449373
1.5877136


046
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.002661762
1.9762497


047
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.000473367
1.9764429


048
203414_at
monocyte to macrophage differentiation-
MMD
NM_012329
0.001585437
1.6128623




associated


049
203553_s_at
mitogen-activated protein kinase kinase kinase
MAP4K5
NM_006575
0.010453912
1.5251595




kinase 5


050
203924_at
glutathione S-transferase A1
GSTA1
NM_000846
0.004046575
4.2017674


051
203988_s_at
fucosyltransferase 8 (alpha (1,6)
FUT8
NM_004480
0.01139016
1.6090198




fucosyltransferase)


052
204426_at
transmembrane emp24 domain trafficking
TMED2
NM_006815
0.015985437
1.6165011




protein 2


053
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.001788037
3.218731




(melanoma growth stimulating activity, alpha)


054
204702_s_at
nuclear factor (erythroid-derived 2)-like 3
NFE2L3
NM_004289
0.015985157
1.7023398


055
204790_at
SMAD, mothers against DPP homolog 7
SMAD7
NM_005904
0.013379821
1.7179344




(Drosophila)


056
204944_at
protein tyrosine phosphatase, receptor type, G
PTPRG
NM_002841
0.004963213
1.769544


057
204989_s_at
integrin, beta 4
ITGB4
BF305661
0.012746719
2.1320713


058
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-
SGCB
U29586
0.013908542
1.7317705




associated glycoprotein)


059
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.000473816
2.054043


060
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.003415823
2.4370956


061
206025_s_at
tumor necrosis factor, alpha-induced protein 6
TNFAIP6
AW188198
0.013965369
2.1515768


062
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.010821017
1.571063


063
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.000283653
2.0841253


064
206245_s_at
influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.003607815
1.5105128


065
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.018292218
1.5056778


066
208510_s_at
peroxisome proliferative activated receptor,
PPARG
NM_015869
0.002361554
1.882336




gamma


067
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.001033398
1.7958127


068
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.000448714
1.631627


069
208653_s_at
CD164 antigen, sialomucin
CD164
AF263279
0.017487219
1.5380286


070
208853_s_at
calnexin
CANX
L18887
0.011792572
1.5100785


071
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.001730693
1.8878508


072
209209_s_at
pleckstrin homology domain containing,
PLEKHC1
AW469573
0.009551367
1.9820172




family C




(with FERM domain) member 1


073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.00507411
1.6641864


074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L
BC001465
0.006051209
1.6464524


075
209409_at
growth factor receptor-bound protein 10
GRB10
D86962
0.01098607
1.7481923


076
209410_s_at
growth factor receptor-bound protein 10
GRB10
AF000017
0.013879589
1.701537


077
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.003979554
1.5687809


078
210845_s_at
plasminogen activator, urokinase receptor
PLAUR
U08839
0.007479298
1.7924315


079
210892_s_at
general transcription factor II,i
GTF2I
BC004472
0.003141172
1.619537


080
210933_s_at
fascin homolog 1, actin-bundling protein
FSCN1
BC004908
0.00342191
1.906748




(Strongylocentrotus purpuratus)


081
210987_x_at
tropomyosin 1 (alpha)
TPM1
M19267
0.004614187
1.6935222


082
211299_s_at
flotillin 2
FLOT2
BC003683
0.015057402
1.5387125


083
211506_s_at
interleukin 8
IL8
AF043337
0.005428782
2.867063


084
211559_s_at
cyclin G2
CCNG2
L49506
0.010491861
1.8367761


085
211599_x_at
met proto-oncogene (hepatocyte growth factor
MET
U19348
0.019789577
1.9247686




receptor)


086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.000418344
1.997547


087
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.00240352
2.8568754


088
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.011889962
1.7860718


089
211924_s_at
plasminogen activator, urokinase receptor
PLAUR
AY029180
0.011789334
1.8189595


090
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.007531395
1.8490748


091
212012_at
peroxidasin homolog (Drosophila)
PXDN
BF342851
0.016265145
1.8463359


092
212660_at
PHD finger protein 15
PHF15
AI735639
0.007391165
1.5595657


093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.016607396
1.5904158


094
212907_at
Solute carrier family 30 (zinc transporter),
SLC30A1
AI972416
0.002460855
1.63999




member 1


095
213288_at
O-acyltransferase (membrane bound) domain
OACT2
AI761250
0.010427832
1.6232696




containing 2


096
213457_at
malignant fibrous histiocytoma amplified
MFHAS1
BF739959
0.003050241
1.8505166




sequence 1


097
213624_at
sphingomyelin phosphodiesterase, acid-like 3A
SMPDL3A
AA873600
0.005912889
1.8562527


098
213742_at
splicing factor, arginine/serine-rich 11
SFRS11
AW241752
0.006011819
1.9170463


099
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
5.50514E−05
1.5048952


100
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.015398935
1.5939685


101
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.003539713
1.8040004


102
214581_x_at
tumor necrosis factor receptor superfamily,
TNFRSF21
BE568134
0.002274355
2.2189345




member 21


103
214701_s_at
fibronectin 1
FN1
AJ276395
0.001182322
2.071262


104
214866_at
plasminogen activator, urokinase receptor
PLAUR
X74039
0.003173471
1.7340106


105
214895_s_at
ADAM metallopeptidase domain 10
ADAM10
AU135154
0.004170008
1.9890832


106
215501_s_at
dual specificity phosphatase 10
DUSP10
AK022513
0.018290011
1.5388945


107
216035_x_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AV721430
0.000657631
1.7091621




HMG-box)


108
216511_s_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AJ270770
0.004103699
1.5264177




HMG-box)


109
216915_s_at
protein tyrosine phosphatase, non-receptor type
PTPN12
S69182
0.005493577
1.6935816




12


110
216971_s_at
plectin 1, intermediate filament binding protein
PLEC1
Z54367
0.01826363
1.7186335




500 kDa


111
217188_s_at
chromosome 14 open reading frame 1
C14orf1
AC007182
0.011925477
1.6185476


112
217448_s_at
chromosome 14 open reading frame 92
C14orf92
AL117508
0.007782524
1.5433311




similar to Epidermal Langerhans cell protein
LOC285412




LCP1


113
217492_s_at
phosphatase and tensin homolog
PTEN
AF023139
0.007220107
1.5624946




(mutated in multiple advanced cancers 1)


114
218000_s_at
pleckstrin homology-like domain, family A,
PHLDA1
NM_007350
0.016502094
1.6960312




member 1


115
218077_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
BE542551
0.01684034
1.5417765


116
218078_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
NM_016598
0.010970607
1.5836283


117
218435_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
NM_013238
0.019865552
1.7292447




15


118
218644_at
pleckstrin 2
PLEK2
NM_016445
0.000675608
2.7071812


119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1
NM_006544
0.012352341
1.7368068


120
218815_s_at
transmembrane protein 51
TMEM51
NM_018022
0.000753902
1.6477742


121
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.009280122
1.6340361


122
218854_at
squamous cell carcinoma
SART2
NM_013352
0.014419112
1.6285655




antigen recognized by T cells 2


123
218856_at
tumor necrosis factor receptor superfamily,
TNFRSF21
NM_016629
0.01292243
1.617686




member 21


124
218885_s_at
UDP-N-acetyl-alpha-D-
GALNT12
NM_024642
0.014052196
1.6402073




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


125
219410_at
transmembrane protein 45A
TMEM45A
NM_018004
0.018847797
2.0938365


126
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.005593323
1.5408667


127
220199_s_at
chromosome 1 open reading frame 80
C1orf80
NM_022831
0.016323
1.5315142


128
220617_s_at
zinc finger protein 532
ZNF532
NM_018181
0.001976648
1.5441327


129
221268_s_at
sphingosine-1-phosphate phosphatase 1
SGPP1
NM_030791
0.008873873
1.9432548


130
221881_s_at
chloride intracellular channel 4
CLIC4
AI638420
0.004401053
1.7742935


131
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.00011337
1.5270268


132
222449_at
transmembrane, prostate
TMEPAI
AL035541
0.005303006
2.2757804




androgen induced RNA


133
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.014745607
1.738053


134
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.005694628
1.5068418


135
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.001075083
1.5835624


136
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.000622161
1.7766397


137
222773_s_at
UDP-N-acetyl-alpha-D-
GALNT12
AA554045
0.003090952
1.8790901




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


138
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.003659447
1.6790042


139
223940_x_at
metastasis associated lung adenocarcinoma
MALAT1
AF132202
0.016841894
1.9524238




transcript 1 (non-coding RNA)


140
224558_s_at
metastasis associated lung adenocarcinoma
MALAT1
AI446756
0.012874936
1.6367766




transcript 1 (non-coding RNA)


141
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.002428954
1.6452742


142
224733_at
chemokine-like factor superfamily 3
CKLFSF3
AL574900
0.013543638
1.5199631


143
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.013437813
1.5261155


144
225021_at
zinc finger protein 532
ZNF532
AA861416
0.002285053
1.6213596


145
225140_at
Kruppel-like factor 3 (basic)
KLF3
BF438116
0.016804362
1.5368354


146
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.006987929
1.5712297


147
225424_at
glycerol-3-phosphate acyltransferase,
GPAM
AB046780
0.000390623
1.7006425




mitochondrial


148
225503_at
dehydrogenase/reductase (SDR family)
DHRSX
AL547782
0.005000754
1.770981




X-linked


149
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.003047524
1.6990312


150
225609_at
glutathione reductase
GSR
AI888037
0.004693668
1.8490914


151
225842_at
Pleckstrin homology-like domain, family A,
PHLDA1
AK026181
0.014052763
1.8735564




member 1


152
226084_at
microtubule-associated protein 1B
MAP1B
AA554833
0.016480966
1.9064581


153
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.001219355
1.5196482


154
226726_at
O-acyltransferase (membrane bound) domain
OACT2
W63676
0.005363467
1.8277074




containing 2


155
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.001859941
1.5185972


156
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.000646104
1.6094143


157
227628_at
similar to RIKEN cDNA 2310016C16
LOC493869
AL571557
0.006222301
2.0978951


158
227808_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
AI091398
0.01153802
1.7936606




15


159
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.005127667
1.6694399


160
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.004784999
1.72546


161
231823_s_at
KIAA1295
KIAA1295
BG054798
0.002478401
1.5713933


162
235587_at
hypothetical protein LOC202781
LOC202781
BG400596
0.018314553
1.5202585


163
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.002645486
2.0540323


164
238558_at
Muscleblind-like (Drosophila)
MBNL1
AI445833
0.004576562
1.805269


165
238563_at
Ab1-interactor 1
ABI1
AV762916
0.012934915
1.6069295


166
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01719282
1.5133282.

14. A microarray for diagnosing a risk of lung cancer recurrence in a lung cancer patient or after a patient has lung cancer treatment, in which at least one probe or probe set specific to each target nucleic acid in a set of target nucleic acids consisting of target nucleic acids in Tables 1 is immobilized on a substrate,
wherein Table 1 is shown below





















Genbank

Fold change


No.
Probe Set ID
Gene Name
Gene Symbol
Accession #
T-test p-value
(abs)





















001
1552486_s_at
lactamase, beta
LACTB
NM_171846
0.005162234
1.522293


002
1553105_s_at
desmoglein 2
DSG2
NM_001943
0.019467462
2.3323212


003
1553530_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_033669
0.01684671
1.7791877




polypeptide,




antigen CD29 includes MDF2, MSK12)


004
1553678_a_at
integrin, beta 1 (fibronectin receptor, beta
ITGB1
NM_133376
0.012459265
1.7374601




polypeptide,




antigen CD29 includes MDF2, MSK12)


005
1554067_at
hypothetical protein FLJ32549
FLJ32549
BC036246
0.002290308
1.5143739


006
1554761_a_at
hypothetical protein FLJ20397
FLJ20397
BC010850
0.001210456
1.6267678


007
1555326_a_at
ADAM metallopeptidase domain 9 (meltrin
ADAM9
AF495383
0.012324799
2.1980886




gamma)


008
1555564_a_at
I factor (complement)
IF
BC020718
0.007528743
2.5875902


009
1555705_a_at
chemokine-like factor superfamily 3
CKLFSF3
AY166714
0.004961676
1.8687251


010
1557987_at
PI-3-kinase-related kinase SMG-1 - like locus
LOC641298
BC042832
0.010989661
1.7944587


011
1558678_s_at
metastasis associated lung adenocarcinoma
MALAT1
BE708432
0.00670648
1.6990829




transcript 1




(non-coding RNA)


012
160020_at
matrix metallopeptidase 14 (membrane-
MMP14
Z48481
0.005463324
1.5193439




inserted)


013
200604_s_at
protein kinase, cAMP-dependent, regulatory,
PRKAR1A
M18468
0.017312625
1.5803499




type I, alpha (tissue specific extinguisher 1)


014
200615_s_at
adaptor-related protein complex 2, beta 1
AP2B1
AL567295
0.007407852
1.6839108




subunit


015
200864_s_at
RAB11A, member RAS oncogene family
RAB11A
NM_004663
0.000163535
1.5653288


016
200922_at
KDEL (Lys-Asp-Glu-Leu) endoplasmic
KDELR1
NM_006801
0.004791257
1.638207




reticulum




protein retention receptor 1


017
201020_at
tyrosine 3-monooxygenase/tryptophan 5-
YWHAH
NM_003405
0.009279575
1.5148095




monooxygenase




activation protein, eta polypeptide


018
201179_s_at
guanine nucleotide binding protein (G protein),
GNAI3
J03005
0.014834337
1.5069977




alpha inhibiting activity polypeptide 3


019
201309_x_at
chromosome 5 open reading frame 13
C5orf13
U36189
0.011555359
2.1326842


020
201363_s_at
influenza virus NS1A binding protein
IVNS1ABP
AB020657
0.00119686
1.5838884


021
201505_at
laminin, beta 1
LAMB1
NM_002291
0.000568398
1.8073287


022
201506_at
transforming growth factor, beta-induced,
TGFBI
NM_000358
0.008768089
1.9059453




68 kDa


023
201548_s_at
Jumonji, AT rich interactive domain 1B
JARID1B
W02593
0.010550437
1.5276276




(RBP2-like)


024
201559_s_at
chloride intracellular channel 4
CLIC4
AF109196
0.002245945
2.1570368


025
201564_s_at
fascin homolog 1, actin-bundling protein
FSCN1
NM_003088
0.007795681
2.1724482




(Strongylocentrotus purpuratus)


026
201578_at
podocalyxin-like
PODXL
NM_005397
0.00303411
1.8943018


027
201617_x_at
caldesmon 1
CALD1
NM_004342
0.01926877
1.8294148


028
201646_at
scavenger receptor class B, member 2
SCARB2 
AA885297
0.006063032
1.6768507


029
201647_s_at
scavenger receptor class B, member 2
SCARB2
NM_005506
0.015885489
1.6841809


030
201695_s_at
nucleoside phosphorylase
NP
NM_000270
0.018524641
1.6833633


031
201722_s_at
UDP-N-acetyl-alpha-D-
GALNT1
AV692127
0.009770202
1.5369248




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 1




(GalNAc-T1)


032
201918_at
Solute carrier family 25, member 36
SLC25A36
AI927944
0.00259865
1.6228764


033
201942_s_at
carboxypeptidase D
CPD
D85390
0.017363481
1.7431495


034
202267_at
laminin, gamma 2
LAMC2
NM_005562
0.004330024
2.8191426


035
202543_s_at
glia maturation factor, beta
GMFB
BC005359
0.008048828
1.5254242


036
202604_x_at
ADAM metallopeptidase domain 10
ADAM10
NM_001110
0.002003783
1.767903


037
202627_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
AL574210
0.00091248
3.0523725




plasminogen activator inhibitor type 1),




member 1


038
202628_s_at
serpin peptidase inhibitor, clade E (nexin,
SERPINE1
NM_000602
0.00504642
2.6835847




plasminogen activator inhibitor type 1),




member 1


039
202817_s_at
synovial sarcoma translocation, chromosome
SS18
NM_005637
0.005462693
1.5148987




18


040
202859_x_at
interleukin 8
IL8
NM_000584
0.014948112
2.1844351


041
202936_s_at
SRY (sex determining region Y)-box 9
SOX9
NM_000346
0.019816045
2.2876046




(campomelic dysplasia, autosomal sex-




reversal


042
202949_s_at
four and a half LIM domains 2
FHL2
NM_001450
0.006776552
2.2249734


043
202998_s_at
lysyl oxidase-like 2
LOXL2
NM_002318
0.006687925
2.0231075


044
203066_at
B cell RAG associated protein
GALNAC4S-
NM_014863
0.00419499
1.5032523





6ST


045
203072_at
myosin IE
MYO1E
NM_004998
0.000449373
1.5877136


046
203293_s_at
lectin, mannose-binding, 1
LMAN1
NM_005570
0.002661762
1.9762497


047
203294_s_at
lectin, mannose-binding, 1
LMAN1
U09716
0.000473367
1.9764429


048
203414_at
monocyte to macrophage differentiation-
MMD
NM_012329
0.001585437
1.6128623




associated


049
203553_s_at
mitogen-activated protein kinase kinase kinase
MAP4K5
NM_006575
0.010453912
1.5251595




kinase 5


050
203924_at
glutathione S-transferase A1
GSTA1
NM_000846
0.004046575
4.2017674


051
203988_s_at
fucosyltransferase 8 (alpha (1,6)
FUT8
NM_004480
0.01139016
1.6090198




fucosyltransferase)


052
204426_at
transmembrane emp24 domain trafficking
TMED2
NM_006815
0.015985437
1.6165011




protein 2


053
204470_at
chemokine (C-X-C motif) ligand 1
CXCL1
NM_001511
0.001788037
3.218731




(melanoma growth stimulating activity, alpha)


054
204702_s_at
nuclear factor (erythroid-derived 2)-like 3
NFE2L3
NM_004289
0.015985157
1.7023398


055
204790_at
SMAD, mothers against DPP homolog 7
SMAD7
NM_005904
0.013379821
1.7179344




(Drosophila)


056
204944_at
protein tyrosine phosphatase, receptor type, G
PTPRG
NM_002841
0.004963213
1.769544


057
204989_s_at
integrin, beta 4
ITGB4
BF305661
0.012746719
2.1320713


058
205120_s_at
sarcoglycan, beta (43 kDa dystrophin-
SGCB
U29586
0.013908542
1.7317705




associated glycoprotein)


059
205180_s_at
ADAM metallopeptidase domain 8
ADAM8
NM_001109
0.000473816
2.054043


060
205479_s_at
plasminogen activator, urokinase
PLAU
NM_002658
0.003415823
2.4370956


061
206025_s_at
tumor necrosis factor, alpha-induced protein 6
TNFAIP6
AW188198
0.013965369
2.1515768


062
206113_s_at
RAB5A, member RAS oncogene family
RAB5A
NM_004162
0.010821017
1.571063


063
206116_s_at
tropomyosin 1 (alpha)
TPM1
NM_000366
0.000283653
2.0841253


064
206245_s_at
influenza virus NS1A binding protein
IVNS1ABP
NM_006469
0.003607815
1.5105128


065
206323_x_at
oligophrenin 1
OPHN1
NM_002547
0.018292218
1.5056778


066
208510_s_at
peroxisome proliferative activated receptor,
PPARG
NM_015869
0.002361554
1.882336




gamma


067
208613_s_at
filamin B, beta (actin binding protein 278)
FLNB
AV712733
0.001033398
1.7958127


068
208637_x_at
actinin, alpha 1
ACTN1
BC003576
0.000448714
1.631627


069
208653_s_at
CD164 antigen, sialomucin
CD164
AF263279
0.017487219
1.5380286


070
208853_s_at
calnexin
CANX
L18887
0.011792572
1.5100785


071
209131_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
U55936
0.001730693
1.8878508


072
209209_s_at
pleckstrin homology domain containing,
PLEKHC1
AW469573
0.009551367
1.9820172




family C




(with FERM domain) member 1


073
209314_s_at
HBS1-like (S. cerevisiae)
HBS1L
AK024258
0.00507411
1.6641864


074
209316_s_at
HBS1-like (S. cerevisiae)
HBS1L
BC001465
0.006051209
1.6464524


075
209409_at
growth factor receptor-bound protein 10
GRB10
D86962
0.01098607
1.7481923


076
209410_s_at
growth factor receptor-bound protein 10
GRB10
AF000017
0.013879589
1.701537


077
209537_at
exostoses (multiple)-like 2
EXTL2
AF000416
0.003979554
1.5687809


078
210845_s_at
plasminogen activator, urokinase receptor
PLAUR
U08839
0.007479298
1.7924315


079
210892_s_at
general transcription factor II,i
GTF2I
BC004472
0.003141172
1.619537


080
210933_s_at
fascin homolog 1, actin-bundling protein
FSCN1
BC004908
0.00342191
1.906748




(Strongylocentrotus purpuratus)


081
210987_x_at
tropomyosin 1 (alpha)
TPM1
M19267
0.004614187
1.6935222


082
211299_s_at
flotillin 2
FLOT2
BC003683
0.015057402
1.5387125


083
211506_s_at
interleukin 8
IL8
AF043337
0.005428782
2.867063


084
211559_s_at
cyclin G2
CCNG2
L49506
0.010491861
1.8367761


085
211599_x_at
met proto-oncogene (hepatocyte growth factor
MET
U19348
0.019789577
1.9247686




receptor)


086
211651_s_at
laminin, beta 1
LAMB1
M20206
0.000418344
1.997547


087
211668_s_at
plasminogen activator, urokinase
PLAU
K03226
0.00240352
2.8568754


088
211864_s_at
fer-1-like 3, myoferlin (C. elegans)
FER1L3
AF207990
0.011889962
1.7860718


089
211924_s_at
plasminogen activator, urokinase receptor
PLAUR
AY029180
0.011789334
1.8189595


090
211981_at
collagen, type IV, alpha 1
COL4A1
NM_001845
0.007531395
1.8490748


091
212012_at
peroxidasin homolog (Drosophila)
PXDN
BF342851
0.016265145
1.8463359


092
212660_at
PHD finger protein 15
PHF15
AI735639
0.007391165
1.5595657


093
212720_at
poly(A) polymerase alpha
PAPOLA
AI670847
0.016607396
1.5904158


094
212907_at
Solute carrier family 30 (zinc transporter),
SLC30A1
AI972416
0.002460855
1.63999




member 1


095
213288_at
O-acyltransferase (membrane bound) domain
OACT2
AI761250
0.010427832
1.6232696




containing 2


096
213457_at
malignant fibrous histiocytoma amplified
MFHAS1
BF739959
0.003050241
1.8505166




sequence 1


097
213624_at
sphingomyelin phosphodiesterase, acid-like 3A
SMPDL3A
AA873600
0.005912889
1.8562527


098
213742_at
splicing factor, arginine/serine-rich 11
SFRS11
AW241752
0.006011819
1.9170463


099
214121_x_at
PDZ and LIM domain 7 (enigma)
PDLIM7
AA086229
5.50514E−05
1.5048952


100
214196_s_at
tripeptidyl peptidase I
TPP1
AA602532
0.015398935
1.5939685


101
214544_s_at
synaptosomal-associated protein, 23 kDa
SNAP23
NM_003825
0.003539713
1.8040004


102
214581_x_at
tumor necrosis factor receptor superfamily,
TNFRSF21
BE568134
0.002274355
2.2189345




member 21


103
214701_s_at
fibronectin 1
FN1
AJ276395
0.001182322
2.071262


104
214866_at
plasminogen activator, urokinase receptor
PLAUR
X74039
0.003173471
1.7340106


105
214895_s_at
ADAM metallopeptidase domain 10
ADAM10
AU135154
0.004170008
1.9890832


106
215501_s_at
dual specificity phosphatase 10
DUSP10
AK022513
0.018290011
1.5388945


107
216035_x_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AV721430
0.000657631
1.7091621




HMG-box)


108
216511_s_at
transcription factor 7-like 2 (T-cell specific,
TCF7L2
AJ270770
0.004103699
1.5264177




HMG-box)


109
216915_s_at
protein tyrosine phosphatase, non-receptor type
PTPN12
S69182
0.005493577
1.6935816




12


110
216971_s_at
plectin 1, intermediate filament binding protein
PLEC1
Z54367
0.01826363
1.7186335




500 kDa


111
217188_s_at
chromosome 14 open reading frame 1
C14orf1
AC007182
0.011925477
1.6185476


112
217448_s_at
chromosome 14 open reading frame 92
C14orf92
AL117508
0.007782524
1.5433311




similar to Epidermal Langerhans cell protein
LOC285412




LCP1


113
217492_s_at
phosphatase and tensin homolog
PTEN
AF023139
0.007220107
1.5624946




(mutated in multiple advanced cancers 1)


114
218000_s_at
pleckstrin homology-like domain, family A,
PHLDA1
NM_007350
0.016502094
1.6960312




member 1


115
218077_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
BE542551
0.01684034
1.5417765


116
218078_s_at
zinc finger, DHHC-type containing 3
ZDHHC3
NM_016598
0.010970607
1.5836283


117
218435_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
NM_013238
0.019865552
1.7292447




15


118
218644_at
pleckstrin 2
PLEK2
NM_016445
0.000675608
2.7071812


119
218748_s_at
SEC10-like 1 (S. cerevisiae)
SEC10L1
NM_006544
0.012352341
1.7368068


120
218815_s_at
transmembrane protein 51
TMEM51
NM_018022
0.000753902
1.6477742


121
218826_at
solute carrier family 35, member F2
SLC35F2
NM_017515
0.009280122
1.6340361


122
218854_at
squamous cell carcinoma
SART2
NM_013352
0.014419112
1.6285655




antigen recognized by T cells 2


123
218856_at
tumor necrosis factor receptor superfamily,
TNFRSF21
NM_016629
0.01292243
1.617686




member 21


124
218885_s_at
UDP-N-acetyl-alpha-D-
GALNT12
NM_024642
0.014052196
1.6402073




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


125
219410_at
transmembrane protein 45A
TMEM45A
NM_018004
0.018847797
2.0938365


126
219603_s_at
zinc finger protein 226
ZNF226
NM_015919
0.005593323
1.5408667


127
220199_s_at
chromosome 1 open reading frame 80
C1orf80
NM_022831
0.016323
1.5315142


128
220617_s_at
zinc finger protein 532
ZNF532
NM_018181
0.001976648
1.5441327


129
221268_s_at
sphingosine-1-phosphate phosphatase 1
SGPP1
NM_030791
0.008873873
1.9432548


130
221881_s_at
chloride intracellular channel 4
CLIC4
AI638420
0.004401053
1.7742935


131
222399_s_at
SM-11044 binding protein
SMBP
BG104571
0.00011337
1.5270268


132
222449_at
transmembrane, prostate
TMEPAI
AL035541
0.005303006
2.2757804




androgen induced RNA


133
222528_s_at
solute carrier family 25, member 37
SLC25A37
BG251467
0.014745607
1.738053


134
222540_s_at
hepatitis B virus x associated protein
HBXAP
BG286920
0.005694628
1.5068418


135
222692_s_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.001075083
1.5835624


136
222693_at
fibronectin type III domain containing 3B
FNDC3B
BF444916
0.000622161
1.7766397


137
222773_s_at
UDP-N-acetyl-alpha-D-
GALNT12
AA554045
0.003090952
1.8790901




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 12




(GalNAc-T12)


138
223577_x_at
PRO1073 protein
PRO1073
AA827878
0.003659447
1.6790042


139
223940_x_at
metastasis associated lung adenocarcinoma
MALAT1
AF132202
0.016841894
1.9524238




transcript 1 (non-coding RNA)


140
224558_s_at
metastasis associated lung adenocarcinoma
MALAT1
AI446756
0.012874936
1.6367766




transcript 1 (non-coding RNA)


141
224674_at
tweety homolog 3 (Drosophila)
TTYH3
AI934753
0.002428954
1.6452742


142
224733_at
chemokine-like factor superfamily 3
CKLFSF3
AL574900
0.013543638
1.5199631


143
224802_at
Nedd4 family interacting protein 2
NDFIP2
AA019338
0.013437813
1.5261155


144
225021_at
zinc finger protein 532
ZNF532
AA861416
0.002285053
1.6213596


145
225140_at
Kruppel-like factor 3 (basic)
KLF3
BF438116
0.016804362
1.5368354


146
225168_at
FERM domain containing 4A
FRMD4A
T78406
0.006987929
1.5712297


147
225424_at
glycerol-3-phosphate acyltransferase,
GPAM
AB046780
0.000390623
1.7006425




mitochondrial


148
225503_at
dehydrogenase/reductase (SDR family)
DHRSX
AL547782
0.005000754
1.770981




X-linked


149
225567_at
Hypothetical LOC388114
LOC388114
BE207755
0.003047524
1.6990312


150
225609_at
glutathione reductase
GSR
AI888037
0.004693668
1.8490914


151
225842_at
Pleckstrin homology-like domain, family A,
PHLDA1
AK026181
0.014052763
1.8735564




member 1


152
226084_at
microtubule-associated protein 1B
MAP1B
AA554833
0.016480966
1.9064581


153
226352_at
Junction-mediating and regulatory protein
JMY
BF447037
0.001219355
1.5196482


154
226726_at
O-acyltransferase (membrane bound) domain
OACT2
W63676
0.005363467
1.8277074




containing 2


155
226780_s_at
hypothetical protein HSPC268
HSPC268
BF540829
0.001859941
1.5185972


156
227257_s_at
chromosome 10 open reading frame 46
C10orf46
AW973842
0.000646104
1.6094143


157
227628_at
similar to RIKEN cDNA 2310016C16
LOC493869
AL571557
0.006222301
2.0978951


158
227808_at
DnaJ (Hsp40) homolog, subfamily C, member
DNAJC15
AI091398
0.01153802
1.7936606




15


159
230206_at
Dedicator of cytokinesis 5
DOCK5
AI692645
0.005127667
1.6694399


160
231735_s_at
PRO1073 protein
PRO1073
NM_014086
0.004784999
1.72546


161
231823_s_at
KIAA1295
KIAA1295
BG054798
0.002478401
1.5713933


162
235587_at
hypothetical protein LOC202781
LOC202781
BG400596
0.018314553
1.5202585


163
235879_at
Muscleblind-like (Drosophila)
MBNL1
AI697540
0.002645486
2.0540323


164
238558_at
Muscleblind-like (Drosophila)
MBNL1
AI445833
0.004576562
1.805269


165
238563_at
Ab1-interactor 1
ABI1
AV762916
0.012934915
1.6069295


166
238701_x_at
FLJ45803 protein
FLJ45803
BE176566
0.01719282
1.5133282.