Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 1 of 26

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, D.C. 20460

Office of Chemical Safety and Pollution Prevention

MEMORANDUM

February 23, 2012

SUBJECT: Acibenzolar-S-Methyl –Acute and Chronic Dietary and Drinking Water 

Exposure and Risk Assessment to support a Section 3 Request for Use of 

Acibenzolar-S-Methyl on Low Growing Berries Crop Subgroup 13-07G.

PC Code: 061402 DP Barcode: 387069

Decision No.: 443561 Registration No.: 100-922

Petition No.: PP#0E7818 Regulatory Action: Section 3 

Risk Assessment Type: Dietary Case No.: NA

TXR No.: NA CAS No.: 135158-54-2

MRID No.: NA 40 CFR: 180.561

FROM: Thurston Morton, Chemist

Risk Assessment Branch IV

Health Effects Division (7509P)

THROUGH: Mohsen Sahafeyan, Chemist

Amelia Acierto, Chemist

Dietary Exposure Science Advisory Council (DESAC), 

Susan Hummel, Chemist

Elissa Reaves PhD, Branch Chief

Risk Assessment Branch IV

Health Effects Division (7509P)

TO: Becky Daiss, Risk Assessor

Risk Assessment Branch 4

Health Effects Division (7509P)

And

Sidney Jackson (RM 05)

Risk Integration, Minor Use & Emergency Response Branch

Registration Division (7505P)
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 2 of 26

This document provides a revised acute and chronic dietary and drinking water risk analyses of 

exposures resulting from use of acibenzolar-S-methyl as a plant activator on food crops. This 

assessment supports a Section 3 request for use of acibenzolar-S-methyl on low growing berries 

Crop Subgroup 13-07G. 

Executive Summary

Acute and chronic dietary risk assessments were conducted for acibenzolar-S-methyl using the 

Dietary Exposure Evaluation Model (DEEM-FCID, Version 2.03), which uses food consumption 

data from the USDA’s Continuing Surveys of Food Intakes by Individuals (CSFII) from 1994-

1996 and 1998. The analyses were performed to support a Section 3 request for use of 

acibenzolar-S-methyl on the low growing berries Crop Subgroup 13-07G.

 

Acute Dietary Exposure Results and Characterization

A refined (probabilistic) acute dietary exposure analysis was performed for the general 

population and all population subgroups. The acute analysis assumed a distribution of residues 

based on field trial data. Empirical and DEEM default processing factors were used to modify

the field trial data. Maximum screening-level percent crop treated estimates were used for 

commodities for which data were available. If no percent crop treated data were available, 100% 

crop treated was assumed. The acute analysis incorporated the 1 in 10 year peak surface drinking 

water estimate from application of acibenzolar-S-methyl to cucurbit vegetables. Acute dietary

risk estimates are not of concern for general population or other population subgroups. The 

subgroup with the highest risk estimate was children 3-5 years old with a 99.9th percentile acute 

exposure estimate of 35% of the aPAD. The 99.9th percentile aPAD for the general U.S. 

population was 17%. 

Chronic Dietary Exposure Results and Characterization

A conservative chronic dietary exposure analysis was performed for the general U.S. population 

and various population subgroups. Tolerance level residues and 100 crop treated assumptions 

were used. DEEM default and empirical processing factors were used to modify the tolerance 

values. The chronic analysis incorporated the 10 year average surface drinking water estimate 

from application of acibenzolar-S-methyl to cucurbit vegetables. The population subgroups 

females 13-49 and young children had a more protective chronic population adjusted dose 

(cPAD, 0.082 mg/kg/day) than the general U.S. population and all other population subgroups 

(0.25 mg/kg/day). Chronic dietary risk estimates are not of concern for general population or 

other population subgroups. The subgroups with the highest risk estimate were children 1-2 and 

3-5 years old with a cPAD of 11%. The cPAD for the general U.S. population was 5%. 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 3 of 26

I. Introduction

Dietary risk assessment incorporates both exposure and toxicity of a given pesticide. For acute 

and chronic assessments, the risk is expressed as a percentage of a maximum acceptable dose 

(i.e., the dose which HED has concluded will result in no unreasonable adverse health effects). 

This dose is referred to as the population adjusted dose (PAD). The PAD is equivalent to the 

Reference Dose (RfD) divided by the FQPA Safety Factor.

For acute and non-cancer chronic exposures, HED is concerned when estimated dietary risk 

exceeds 100% of the PAD. References that discuss the acute and chronic risk assessments in 

more detail are available on the EPA/pesticides web site: “Available Information on Assessing 

Exposure from Pesticides, A User’s Guide,” 6/21/2000, web link: 

http://www.epa.gov/fedrgstr/EPA_PEST/2000/July/Day_12/6061.pdf ; or see SOP 99.6 

(8/20/99).

II. Residue Information

Residues of Concern 

The HED Metabolism Assessment Review Committee (MARC) reviewed lettuce, tomato, 

cotton, lemon, poultry, and ruminant metabolism studies and the environmental fate data. Table 

1 provides a summary of the MARC decisions regarding residues of concern for acibenzolar-Smethyl. (D261037, M. Doherty, 4/26/00)

Table 1: Summary of MARC Decisions for Acibenzolar-S-methyl

Matrix Residues of Concern 1

For Risk Assessment 2 For Tolerance Expression

Plants Residues of acibenzolar-S-methyl, benzo(1,2,3) 

thiadiazole-7-carbothioic acid-S-methyl ester, 

convertible to benzo(1,2,3)thiadiazole-7-carboxylic acid 

(CGA-210007), expressed as acibenzolar-S-methyl &

4-hydroxy CGA-210007 (CGA-323060)

5-hydroxy CGA-210007 (CGA-324041)

Residues of acibenzolar-S-methyl, 

benzo(1,2,3) thiadiazole-7-carbothioic 

acid-S-methyl ester, convertible to 

benzo(1,2,3)thiadiazole-7-carboxylic 

acid (CGA-210007), expressed as 

acibenzolar-S-methyl

1

The residues of concern for drinking water are the same as those listed in the tolerance expression

2

If a future petition is received for rice, residues of CGA-379019 should be included in the risk assessment
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 4 of 26

Established/Recommended Tolerances

Tolerances have been established (40CFR 180.561) for residues of acibenzolar-S- methyl 

(benzo(1,2,3)thiadiazole-7-carbothioic acid-S-methyl ester) in/on spinach at 1.0 ppm, leafy

vegetables (Crop Group 4, other than spinach, at 0.25 ppm), Brassica (cole) leafy vegetables 

(Crop Group 5 at 1.0 ppm), fruiting vegetables (Crop Group 8 at 1.0 ppm), and tomato paste at 

3.0 ppm. There is also an import tolerance for bananas at 0.1 ppm. Tolerances have been 

recently established for the bulb onion subgroup 3-07A at 0.1 ppm and the cucurbit vegetable 

crop group 9 at 2.0 ppm. HED is recommending for a tolerance of 0.15 ppm for residues of 

acibenzolar-S-methyl in/on the low growing berry subgroup 13-07G. 

The tolerance expression for acibenzolar-S-methyl is for residues convertible to 

benzo(1,2,3)thiadiazole-7-carboxylic acid (CGA-210007), expressed as acibenzolar-S-methyl. 

Residues Used in the Chronic Analysis

In the chronic dietary exposure analysis, tolerance level residues were used and 100 percent crop 

treated was assumed for all commodities. DEEM default processing factors were used for dried 

onion, dried banana, dried plantain, and dried tomato. A processing factor was not used for 

tomato paste because a separate tolerance has been established for this processed commodity. In 

the submitted tomato processing study (D246673, M. Doherty, 3/15/2000), reported processing 

factors of 1.0 and 2.9 for tomato juice and tomato puree were used. 

Available lettuce metabolism data for a 7-day PHI were used to determine the relative abundance 

of the hydroxy metabolites (CGA-323060 and CGA-324041) and residues convertible to the 

carboxylic acid metabolite (CGA-210007). Based on the lettuce metabolism data, a factor of 

1.5x was applied to estimates of acibenzolar-S-methyl residues to account for all of the residues 

of concern for dietary risk (including CGA-210007, CGA-323060 and CGA-324041). Lettuce 

metabolism data are provided in Table 2. 

Table 2. Lettuce metabolism data (Washed, 7-day PHI)

Compound % Residues

Acibenzolar-S-Methyl 17

CGA-210007 25

CGA-323060 20

CGA-324041 0.06

Residues Used in the Acute Analysis

A probabilistic assessment was performed for the acute analysis. The acute analysis assumed a 

distribution of residues based on field trial data for nonblended and partially blended 

commodities. For blended commodities, the mean field trial values were used as a point 

estimate. A value of 1⁄2 LOQ was used for samples that contained less than LOQ residues. Foods 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 5 of 26

were classified as blended, partially blended, or nonblended based on guidance given in the Draft 

DEEM-FCID Classification of Food Forms with Respect to Blending (F. Fort, 4/9/2003). DEEM 

default processing factors were used for dried onion, dried banana, dried plantain, and dried 

tomato. Empirical processing factors were used for tomato paste (7.1), tomato puree (2.9), and 

tomato juice (1.0). The acute analysis used available maximum percent crop treated estimates

from the Biological and Economic Analysis Division’s (BEAD’s) screening-level analysis 

(D353541, J. Alsadek, 6/18/08). If no percent crop treated data were available 100% crop treated 

was assumed. As previously noted, a factor of 1.5X was applied to the estimate of residues 

convertible to CGA-210007 for all commodities to account for all of the residues of concern for 

dietary risk. 

Residues used in the acute and chronic dietary analyses are provided in Table 3.
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 6 of 26

Table 3. Data and Residue Estimates Used in Acibenzolar-S-Methyl Dietary and Drinking Water Analyses

Commodity

Blended

Status 1 Data

Source

%CT Process

Factors

Anticipated Residue 

Estimates/Tolerance 2

Avg Max Acute Chronic

(Tolerance)

Crop Group 3-07A Onions

Onion, dry bulb and

dry bulb-babyfood NB/PB Field Trials

14 samples

1 detectable residue 

MRID47375301

100 100

NA

RDF# 23

Onion (100% CT)

TOTALZ=0

TOTALNZ=7 0.1

Onion , dry bulb dried, and driedbabyfood B

9

Tolerance 0.1

Onion , Green Onion NA

Crop Group 4A – Leafy Greens

Amaranth, Arugula, Cress

Chrysanthemum , Endive, 

Dandelions, Parsley PB

Field Trails

11 samples

11 detectable residues

MRID 44537062

100 100 NA

RDF# 18

SpinichOth (100% CT)

TOTALZ=0

TOTALNZ=11

0.25

Lettuce, head 

NB/PB

Field Trails

12 samples

12 detectable residues

MRID 44537062

5 15 NA

RDF# 6

LettuceH (12% CT)

TOTALZ=88

TOTALNZ=12

0.25

Lettuce, leaf 

PB

Field Trails

11 samples

11 detectable residues

MRID 44537062

5 15 NA

RDF# 8

LettuceL (12% CT)

TOTALZ=81

TOTALNZ=11

0.25

Radicchio

PB

Field Trails

12 samples

12 detectable residues

MRID 44537062

100 100 NA

RDF# 16

LettuceHRad(12% CT)

TOTALZ=0

TOTALNZ=12

0.25

Spinach

PB

Field Trails

11 samples

11 detectable residues

MRID 44537062

25 40 NA

RDF# 10

Spinach (30% CT)

TOTALZ=26

TOTALNZ=11

1.0

Crop Group 4B – Leafy Petioles

Cardoon

Celtuce

Fennel

Rhubarb 

Swiss chard

NB

Field Trails

9 samples

9 detectable residues

MRID 44537062

100 100 NA

RDF# 15

CeleryOth (100% CT)

TOTALZ=0

TOTALNZ=9

0.25

Celery

NB/PB

Field Trails

9 samples

9 detectable residues

MRID 44828007

100 100 NA

RDF# 15

Celery (100% CT)

TOTALZ=0

TOTALNZ=9

0.25

Crop Group 5A – Brassica Head & Stem

Broccoli

Cauliflower NB/PB

Field Trails

11 samples

11 detectable residues

MRID 44828007

5

5

5

10

NA

RDF# 3

Broccoli (5% CT)

TOTALZ=209

TOTALNZ=11

1.0
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 7 of 26

Table 3. Data and Residue Estimates Used in Acibenzolar-S-Methyl Dietary and Drinking Water Analyses

Commodity

Blended

Status 1 Data

Source

%CT Process

Factors

Anticipated Residue 

Estimates/Tolerance 2

Avg Max Acute Chronic

(Tolerance)

Cabbage

NB/PB

Field Trails

11 samples

11 detectable residues

MRID 44828007

1 1 NA

RDF# 4

Cabbage (2.5% CT)

TOTALZ=429

TOTALNZ=11

1.0

Cabbage Chinese Mustard

Brocolli Chinese

NB

Field Trails

11 samples

11 detectable residues

MRID 44828007

100 100 NA

RDF# 13

BroccoliOth (100% CT)

TOTALZ=0

TOTALNZ=11

1.0

Kohlrabi

Brussels sprouts

Cabbage Chinese Napa NB

Field Trails

11 samples

11 detectable residues

MRID 44828007

100 100 NA

RDF# 14

CabbageOth (100% CT)

TOTALZ=0

TOTALNZ=11

1.0

Crop Group 5B– Brassica Leafy Greens

Cabbage Chinese bok choy 

Collards

Kale

Mustard Greens

Rape greens 

Turnip greens 

NB

Field Trails

9 samples

9 detectable residues

MRID 44828007

100 100 NA

RDF# 9

Mustard (100% CT)

TOTALZ=0

TOTALNZ=9

1.0

Crop Group 8 – Fruiting Vegetables

Eggplant, 

NB/PB

Field Trails

9 samples

9 detectable residues

MRID 44537060

100 100 NA

RDF# 12

BellPepOth (100% CT)

TOTALZ=0

TOTALNZ=9

1.0

Okra

NB/PB

Field Trails

9 samples

9 detectable residues

MRID 44537060

100 100 NA

RDF# 17

HotPepOth (100% CT)

TOTALZ=0

TOTALNZ=9

1.0

Pepper nonbell 

NB/PB

Field Trails

5 samples

5 detectable residues

MRID 44537060

5 10 NA

RDF# 7

HotPep (5% CT)

TOTALZ=95

TOTALNZ=5

1.0

Pepper nonbell , dried

B

Field Trails

5 samples

5 detectable residues

MRID 44537060

5 10 NA Mean field trial (0.321) 1.0

Pepper, bell 

NB/PB

Field Trails

9 samples

9 detectable residues

MRID 44537060

5 10 NA

RDF# 2

BellPep (5% CT)

TOTALZ=179

TOTALNZ=9

1.0

Tomatillo

NB/PB

Field Trails

23 samples

23 detectable residues

MRID 44537060

2.5 5 NA

RDF# 19

TomatoOth(100% CT)

TOTALZ=0

TOTALNZ=23

1.0
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 8 of 26

Table 3. Data and Residue Estimates Used in Acibenzolar-S-Methyl Dietary and Drinking Water Analyses

Commodity

Blended

Status 1 Data

Source

%CT Process

Factors

Anticipated Residue 

Estimates/Tolerance 2

Avg Max Acute Chronic

(Tolerance)

Tomato

NB/PB

Field Trails

23 samples

23 detectable residues

MRID 44537060

2.5 5 NA

RDF# 11

Tomato(5% CT)

TOTALZ=437

TOTALNZ=23

1.0

Tomato, paste, 

B

Field Trails

23 samples

23 detectable residues

MRID 44537060

2.5 5 7.1 Mean field trial: 0.164 1.0

Tomato, puree 

B

Field Trails

23 samples

23 detectable residues

MRID 44537060

2.5 5 2.9 Mean field trial: 0.164 1.0

Tomato, dried 

B

Field Trails

23 samples

23 detectable residues

MRID 44537060

2.5 5 14.3 Mean field trial: 0.164 1.0

Crop Group 9A– Cucurbit Vegetables - Melon

Cantaloupe, 

Casaba, 

Honeydew melon, 

Watermelon 

NB/PB

Field Trails

12 samples

12 detectable residues

MRID 45105301

100 100 NA

RDF# 20

Cantaloupe(100% CT)

TOTALZ=0

TOTALNZ=6

2.0

Crop Group 9B – Cucurbit Vegetables – Squash Cucumbers

Chayote, fruit, Chinese waxgourd NB NA 2.0

Cucumber, Balsam pear 

 

NB

Field Trails

16 samples

16 detectable residues

MRID 45105301

100 100 NA

RDF# 21

Cucumber(100% CT)

TOTALZ=0

TOTALNZ=8

2.0

Pumpkin, Squash 

NB/PB

Field Trails

10 samples

10 detectable residues

MRID 45105301

100 100 NA

RDF# 22

Squash(100% CT)

TOTALZ=0

TOTALNZ=5

2.0

Crop Group O - Other

Banana, Plantain

NB/PB

Field Trails

30 samples

8 detectable residues

MRID 44828004

100 100 NA

RDF#1

Banana(100%CT)

Total Z=0

TotalNZ=30

0.1

Banana, Plantain, dried

B

Field Trails

30 samples

8 detectable residues

MRID 44828004

100 100 3.9 Mean field trial (0.0107) 0.1

Water NA D351609 100 100 NA 0.017 0.011

1

Classification of blended (B), partially blended (PB), not blended (NB).

2 A factor of 1.5X was applied to estimate residues convertible to CGA-210007 for all commodities to account for all of the residues of concern for 

dietary risk. 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 9 of 26

III. Water Data

Drinking water residues provided by the Environmental Fate and Effects Division (EFED) 

(D353745, R. Miller, 9/2/10) were incorporated directly into the acute and chronic dietary

analyses. The surface water estimates were based on EFED’s Tier II model Pesticide Root Zone 

Model. The residues of concern for drinking water include acibenzolar-S-methyl and residues 

convertible to CGA-210007. The estimated EDWCs for use of acibenzolar-S-methyl on

cucumbers were used for the drinking water assessment because this use represents the highest 

use rate of the existing and proposed registered uses. The SCI-GROW estimated ground water 

drinking water concentrations for acibenzolar is not expected to exceed 1.25 x 10-5μg/L and 

CGA 210007 is not expected to exceed 8.12 x 10-2μg/L. Surface water EDWCs for acibenzolarS-methyl, which were used in the exposure assessment, are provided in Table 4. CGA-210007 

drinking water residues were included in the dietary exposure assessment as acibenzolar-Smethyl equivalents.

Table 4. EDWCs for use in screening the human health risk assessment (FL Cucumber)

Drinking Water 

Source Chemical 1-in-10 year 

annual acute 

conc. (μg/L)

1-in-10 year avg 

annual (chronic) 

concentration (μg/L)

30-year avg. annual 

concentration(μg/L)

Surface Water Acibenzolar-S-Methyl1

0.72 0.02 0.02

Surface Water CGA 2100072

20.02 8.09 4.68

1 Aerial application method

2 Degradation product formed after application 

CGA-210007 drinking water residues were included in the dietary exposure assessment as 

acibenzolar-S-methyl equivalents. CGA 210007 residues were converted based on molecular 

weight as follows. 

Acute: The acute time distribution was adjusted to account for the metabolite.

Chronic: 210 ÷ 180 x 8.09 (μg/L) = 9.44 μg/L CGA 210007 

IV. DEEM-FCIDTM Program and Consumption Information

Acibenzolar-S-Methyl acute and chronic dietary exposure assessments were conducted using the 

Dietary Exposure Evaluation Model software with the Food Commodity Intake Database 

(DEEM-FCIDTM, Version 2.03), which incorporates consumption data from USDA’s Continuing 

Surveys of Food Intakes by Individuals (CSFII), 1994-1996 and 1998. The 1994-96, 98 data are 

based on the reported consumption of more than 20,000 individuals over two non-consecutive 

survey days. Foods “as consumed” (e.g., apple pie) are linked to EPA-defined food commodities 

(e.g. apples, peeled fruit - cooked; fresh or N/S; baked; or wheat flour - cooked; fresh or N/S, 

baked) using publicly available recipe translation files developed jointly by USDA/ARS and 

EPA. For chronic exposure assessment, consumption data are averaged for the entire U.S. 

population and within population subgroups, but for acute exposure assessment are retained as 

individual consumption events. Based on analysis of the 1994-96, 98 CSFII consumption data, 

which took into account dietary patterns and survey respondents, HED concluded that it is most 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 10 of 26

appropriate to report risk for the following population subgroups: the general U.S. population, all 

infants (<1 year old), children 1-2, children 3-5, children 6-12, youth 13-19, adults 20-49, 

females 13-49, and adults 50+ years old.

For chronic dietary exposure assessment, an estimate of the residue level in each food or foodform (e.g., orange or orange juice) on the food commodity residue list is multiplied by the 

average daily consumption estimate for that food/food form. The resulting residue consumption 

estimate for each food/food form is summed with the residue consumption estimates for all other 

food/food forms on the commodity residue list to arrive at the total average estimated exposure. 

Exposure is expressed in mg/kg body weight/day and as a percent of the cPAD. This procedure 

is performed for each population subgroup.

For acute exposure assessments, individual one-day food consumption data are used on an 

individual-by-individual basis. The reported consumption amounts of each food item can be 

multiplied by a residue point estimate and summed to obtain a total daily pesticide exposure for a 

deterministic exposure assessment, or “matched” in multiple random pairings with residue values 

and then summed in a probabilistic assessment. The resulting distribution of exposures is 

expressed as a percentage of the aPAD on both a user (i.e., those who reported eating relevant 

commodities/food forms) and a per-capita (i.e., those who reported eating the relevant 

commodities as well as those who did not) basis. In accordance with HED policy, per capita 

exposure and risk are reported for all tiers of analysis. However, for tiers 1 and 2, significant 

differences in user vs. per capita exposure and risk are identified and noted in the risk 

assessment.

V. Toxicological Information

HED evaluated the toxicology data base for acibenzolar-S-methyl and selected the following doses 

and endpoints for acute and chronic dietary and drinking water risk assessments. 

[Note: In the previous dietary exposure assessment risk assessment (D362208, 3/3/09), only the 

subpopulation females 13-50 years old was assessed for acute dietary exposure based on in utero 

effects. Additionally, only the subpopulation females 13-50 years old was assessed for chronic 

dietary exposure based on in utero effects observed in the DNT study. However, based on a 

reevaluation of the DNT study regarding the potential for post-natal toxicity, the acute dietary

analysis has been expanded to include children for this assessment and the the chronic dietary

analysis has been revised to include an assessment of dietary risks to children using the NOAEL 

from the DNT study.]
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 11 of 26

Table 5. Summary of Toxicological Doses and Endpoints for Acibenzolar-S-methyl for Use in Dietary and Non-Occupational 

Human Health Risk Assessments

Exposure/Scenario Point of 

Departure

Uncertainty/FQPA 

Safety Factors

RfD, PAD, LOC 

for Risk 

Assessment

Study and Toxicological Effects

Acute Dietary 

(General Population)

NOAEL = 8.2 

mg/kg/day

UFA= 10x

UFH=10x

FQPA SF= 1x

Acute RfD = 

0.082 mg/kg/day

aPAD = 0.082

mg/kg/day

Developmental Neurotoxicity Toxicity Study - Rat 

Developmental LOAEL = 82 mg/kg/day based on 

changes in brain morphometrics in the cerebellum in 

offspring. 

Maternal LOAEL = was not observed NOAEL = 

4000 mg/kg/day HDT

Chronic Dietary 

(Females 13-49 years 

& Young Children)

NOAEL = 8.2 

mg/kg/day

UFA= 10x

UFH=10x

FQPA SF= 1x

Acute RfD = 

0.082 mg/kg/day

cPAD = 0.082

mg/kg/day

Developmental Neurotoxicity Toxicity Study - Rat 

Developmental LOAEL = 82 mg/kg/day based on 

changes in brain morphometrics in the cerebellum in 

offspring. 

Maternal LOAEL = was not observed 

NOAEL = 4000 mg/kg/day HDT

Chronic Dietary 

(Adult Males and 

Females 50+ yrs)

NOAEL = 25 

mg/kg/day

UFA= 10x

UFH=10x

FQPA SF= 1x

Chronic RfD = 

0.025

mg/kg/day

cPAD = 0.25

mg/kg/day

Chronic Toxicity - Dog; Co-critical; Chronic/Cancer -

Rat & Mouse, Reproduction Toxicity - Rat 

LOAEL = 25 mg/kg/day based on hemolytic anemia 

with compensatory response. 

Cancer (all routes) Not a Human Carcinogen

Point of Departure (POD) = A data point or an estimated point that is derived from observed dose-response data and used to mark the beginning of 

extrapolation to determine risk associated with lower environmentally relevant human exposures. NOAEL = no observed adverse effect level. 

NOAEL = no observed adverse effect level. 

LOAEL = lowest observed adverse effect level. 

UF = uncertainty factor. 

UFA = extrapolation from animal to human (interspecies). 

UFH = potential variation in sensitivity among members of the human population (intraspecies). 

FQPA SF = FQPA Safety Factor. 

PAD = population adjusted dose (a = acute, c = chronic). 

RfD = reference dose. 

VI. Results/Discussion

As stated above, for acute and chronic assessments, HED is concerned when dietary risk exceeds 

100% of the PAD. The DEEM-FCIDTM analyses estimate the dietary exposure of the U.S. 

population and various population subgroups. The results reported in Tables 6-8 are for the 

general U.S. Population, all infants (<1 year old), children 1-2, children 3-5, children 6-12, youth 

13-19, females 13-49, adults 20-49, and adults 50+ years.

Results of Acute Dietary Exposure Analysis

The results of the acute dietary exposure analysis at the 99.9th percentile of exposure are reported 

in Table 6.
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 12 of 26

Table 6: Summary of Acute Dietary Exposure and Risk (Food and Water) for Acibenzolar-SMethyl

Population Subgroup aPAD (mg/kg/day) Acute (99.9th Percentile)

Exposure (mg/kg/day) % aPAD

General U.S. Population

0.082

0.014120 17

All Infants (< 1 year old) 0.013034 16

Children 1-2 years old 0.025051 31

Children 3-5 years old 0.028600 35

Children 6-12 years old 0.019931 24

Youth 13-19 years old 0.015067 18

Adults 20-49 years old 0.009085 11

Adults 50+ years old 0.010651 13

Females 13-49 years old 0.009561 12

Results of Chronic Dietary Exposure Analysis

The results of the chronic dietary exposure analysis are reported in Tables 7 and 8. 

Table 7: Summary of Chronic Dietary Exposure and Risk to Female and Child Population for 

Acibenzolar-S-Methyl 

Population Subgroup cPAD (mg/kg/day)

Chronic

Exposure (mg/kg/day) % cPAD

Females 13-49 years old

0.082

0.003646 4

All Infants (< 1 year old) 0.003808 5

Children 1-2 years old 0.009207 11

Children 3-5 years old 0.008706 11

Children 6-12 years old 0.005812 7

Table 8: Summary of Chronic Dietary Exposure and Risk to Adults 50+ and Adult Males (13+) 

Acibenzolar-S-Methyl

Population Subgroup cPAD (mg/kg/day) Chronic

Exposure (mg/kg/day) % cPAD

General U.S. Population

0.25

0.004366 5

Youth 13-19 0.003893 2

Adults 20-49 0.003654 2

Adults 50+ years old 0.003852 2

VI. Characterization of Inputs/Outputs

The chronic dietary analysis is very conservative. Tolerance level residues used in the analysis 

are expected to exceed the residues actually consumed. In addition, the 100% crop treated 

estimate used for all crops in the assessment will considerably overestimate the actual percent 

crop treated. 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 13 of 26

The acute dietary analysis is refined. The use of field trial data and BEAD’s maximum percent 

crop treated estimates in a probabilistic assessment provided exposure estimates that are more 

likely to be representative of actual exposures. These estimates could be further refined, however

by use of percent crop treated estimates for the crops for which 100% crop treated assumptions 

were made, use of monitoring data, market basket surveys, washing factors, and cooking factors. 

To further characterize the exposure of acibenzolar-s-methyl residues, HED conducted a CSA. 

The purpose of the CSA calculations is to inform risk managers on the range of exposures that an 

individual may obtain from eating a treated commodity. For acibenzolar-s-methyl, HED 

estimated the risk to a 15 kg toddler consuming a reasonable serving of strawberry or blueberry

with residues at the tolerance. The resulting risk estimates were 2% and 1% of the aPAD for 

strawberry and blueberry, respectively.

VII. Conclusions

A conservative chronic dietary exposure analysis was performed for the general U.S. population 

and various population subgroups. The risk estimates for the general U.S. population and all 

population subgroups are below HED’s level of concern. HED is confident that acute and 

chronic dietary exposures to acibenzolar-S-methyl are not underestimated.

VIII. List of Attachments

1. Acute DEEM-FCID Residue Input File.

2. Results of Acute DEEM-FCID Dietary Exposure Analysis.

3. Chronic DEEM-FCID Residue Input File.

4. Results of Chronic DEEM-FCID Dietary Exposure Analysis.

5. Percent Crop Treated Estimates Provided by BEAD

6. RDF Files Used in Acute Analysis
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 14 of 26

Attachment 1 Acute DEEM-FCID Residue Input File

Filename: C:\Documents and Settings\tmorton\My Documents\MyFiles\DEEM Files\061402 

Acibenzolar\Acibenzolar on Low Growing Berries\061402Acute.R98

Chemical: Acibenzolar-S-Methyl

RfD(Chronic): .082 mg/kg bw/day NOEL(Chronic): 0 mg/kg bw/day

RfD(Acute): .082 mg/kg bw/day NOEL(Acute): 0 mg/kg bw/day

Date created/last modified: 06-24-2011/15:03:28/8 Program ver. 2.03

--------------------------------------------------------------------------------

RDL indices and parameters for Monte Carlo Analysis:

Index Dist Parameter #1 Param #2 Param #3 Comment

 # Code 

----- ---- ------------ -------- -------- -------

 1 6 Banana.rdf

 2 6 BellPep.rdf

 3 6 Broccoli.rdf

 4 6 Cabbage.rdf

 5 6 Celery.rdf

 6 6 LettuceH.rdf

 7 6 HotPep.rdf

 8 6 LettuceL.rdf

 9 6 Mustard.rdf

 10 6 Spinach.rdf

 11 6 Tomato.rdf

 12 6 BellPepOth.rdf

 13 6 BroccoliOth.rdf

 14 6 CabbageOth.rdf

 15 6 CeleryOth.rdf

 16 6 LettuceHRad.rdf

 17 6 HotPepOth.rdf

 18 6 SpinachOth.rdf

 19 6 TomatoOth.rdf

 20 6 Cantaloupe.rdf

 21 6 Cucumber.rdf

 22 6 Squash.rdf

 23 6 Onion.rdf

 24 6 Low Growing Berries.rdf

 25 6 Cauliflower.rdf

 26 6 DrinkingWaterAcibenzolarPlusConvertedMetabolite.rdf

 EPA Crop Commodity Name Def Res Adj.Factors RDL Comment

 Code Grp (ppm) #1 #2 Pntr

-------- ---- ------------------------------- ---------- ------ ------ --- -------

03002370 3 Onion, dry bulb 0.100000 1.000 1.500 23 

03002371 3 Onion, dry bulb-babyfood 0.100000 1.000 1.500 23 

03002380 3 Onion, dry bulb, dried 0.029000 9.000 1.500 

03002381 3 Onion, dry bulb, dried-babyfood 0.029000 9.000 1.500 

03002390 3 Onion, green 0.100000 1.000 1.500 23 

03003380 3 Shallot 0.100000 1.000 1.500 23 

04010050 4A Amaranth, leafy 0.250000 1.000 1.500 18 

04010180 4A Arugula 0.250000 1.000 1.500 18 

04011040 4A Chrysanthemum, garland 0.250000 1.000 1.500 18 

04011330 4A Cress, garden 0.250000 1.000 1.500 18 

04011340 4A Cress, upland 0.250000 1.000 1.500 18 

04011380 4A Dandelion, leaves 0.250000 1.000 1.500 18 

04011500 4A Endive 0.250000 1.000 1.500 18 

04012040 4A Lettuce, head 0.250000 1.000 1.500 6 

04012050 4A Lettuce, leaf 0.250000 1.000 1.500 8 

04012480 4A Parsley, leaves 0.250000 1.000 1.500 18 

04013130 4A Radicchio 0.250000 1.000 1.500 16 

04013550 4A Spinach 1.000000 1.000 1.500 10 

04013551 4A Spinach-babyfood 1.000000 1.000 1.500 10 

04020760 4B Cardoon 0.250000 1.000 1.500 15 

04020850 4B Celery 0.250000 1.000 1.500 5 

04020851 4B Celery-babyfood 0.250000 1.000 1.500 5 

04020860 4B Celery, juice 0.250000 1.000 1.500 5 

04020870 4B Celtuce 0.250000 1.000 1.500 15 

04021520 4B Fennel, Florence 0.250000 1.000 1.500 15 

04023220 4B Rhubarb 0.250000 1.000 1.500 15 

04023670 4B Swiss chard 0.250000 1.000 1.500 15 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 15 of 26

05010610 5A Broccoli 1.000000 1.000 1.500 3 

05010611 5A Broccoli-babyfood 1.000000 1.000 1.500 3 

05010620 5A Broccoli, Chinese 1.000000 1.000 1.500 13 

05010640 5A Brussels sprouts 1.000000 1.000 1.500 14 

05010690 5A Cabbage 1.000000 1.000 1.500 4 

05010710 5A Cabbage, Chinese, napa 1.000000 1.000 1.500 14 

05010720 5A Cabbage, Chinese, mustard 1.000000 1.000 1.500 13 

05010830 5A Cauliflower 1.000000 1.000 1.500 25 

05011960 5A Kohlrabi 1.000000 1.000 1.500 14 

05020630 5B Broccoli raab 1.000000 1.000 1.500 9 

05020700 5B Cabbage, Chinese, bok choy 1.000000 1.000 1.500 9 

05021170 5B Collards 1.000000 1.000 1.500 9 

05021940 5B Kale 1.000000 1.000 1.500 9 

05022290 5B Mustard greens 1.000000 1.000 1.500 9 

05023180 5B Rape greens 1.000000 1.000 1.500 9 

05023890 5B Turnip, greens 1.000000 1.000 1.500 9 

08001480 8 Eggplant 1.000000 1.000 1.500 12 

08002340 8 Okra 1.000000 1.000 1.500 17 

08002700 8 Pepper, bell 1.000000 1.000 1.500 2 

08002701 8 Pepper, bell-babyfood 1.000000 1.000 1.500 2 

08002710 8 Pepper, bell, dried 0.321000 1.000 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08002711 8 Pepper, bell, dried-babyfood 0.321000 1.000 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08002720 8 Pepper, nonbell 1.000000 1.000 1.500 7 

08002721 8 Pepper, nonbell-babyfood 1.000000 1.000 1.500 7 

08002730 8 Pepper, nonbell, dried 0.358000 1.000 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003740 8 Tomatillo 1.000000 1.000 1.500 19 

08003750 8 Tomato 1.000000 1.000 1.500 11 

08003751 8 Tomato-babyfood 1.000000 1.000 1.500 11 

08003760 8 Tomato, paste 0.164000 7.100 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003761 8 Tomato, paste-babyfood 0.164000 7.100 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003770 8 Tomato, puree 0.164000 2.900 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003771 8 Tomato, puree-babyfood 0.164000 2.900 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003780 8 Tomato, dried 0.164000 14.300 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003781 8 Tomato, dried-babyfood 0.164000 14.300 0.075 1.5 x 

 Full comment: 1.5 x 5%CT

08003790 8 Tomato, juice 1.000000 1.000 1.500 11 

09010750 9A Cantaloupe 1.000000 1.000 1.500 20 

09010800 9A Casaba 1.000000 1.000 1.500 20 

09011870 9A Honeydew melon 1.000000 1.000 1.500 20 

09013990 9A Watermelon 1.000000 1.000 1.500 20 

09014000 9A Watermelon, juice 1.000000 1.000 1.500 20 

09020210 9B Balsam pear 1.000000 1.000 1.500 21 

09020880 9B Chayote, fruit 1.000000 1.000 1.500 22 

09021020 9B Chinese waxgourd 1.000000 1.000 1.500 21 

09021350 9B Cucumber 1.000000 1.000 1.500 22 

09023080 9B Pumpkin 1.000000 1.000 1.500 22 

09023090 9B Pumpkin, seed 1.000000 1.000 1.500 22 

09023560 9B Squash, summer 1.000000 1.000 1.500 22 

09023561 9B Squash, summer-babyfood 1.000000 1.000 1.500 22 

09023570 9B Squash, winter 1.000000 1.000 1.500 22 

09023571 9B Squash, winter-babyfood 1.000000 1.000 1.500 22 

13020570 13B Blueberry 0.150000 1.000 1.500 24 

13020571 13B Blueberry-babyfood 0.150000 1.000 1.500 24 

86010000 O Water, direct, all sources 0.017000 1.000 1.000 26 

86020000 O Water, indirect, all sources 0.017000 1.000 1.000 26 

95000230 O Banana 0.100000 1.000 1.500 1 

95000231 O Banana-babyfood 0.100000 1.000 1.500 1 

95000240 O Banana, dried 0.010700 3.900 1.500 

95000241 O Banana, dried-babyfood 0.010700 3.900 1.500 

95001300 O Cranberry 0.150000 1.000 1.500 24 

95001301 O Cranberry-babyfood 0.150000 1.000 1.500 24 

95001310 O Cranberry, dried 0.150000 1.000 1.500 24 

95001320 O Cranberry, juice 0.150000 1.000 1.500 24 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 16 of 26

95001321 O Cranberry, juice-babyfood 0.150000 1.000 1.500 24 

95002830 O Plantain 0.100000 1.000 1.500 1 

95002840 O Plantain, dried 0.010700 3.900 1.500 

95003590 O Strawberry 0.150000 1.000 1.500 24 

95003591 O Strawberry-babyfood 0.150000 1.000 1.500 24 

95003600 O Strawberry, juice 0.150000 1.000 1.500 24 

95003601 O Strawberry, juice-babyfood 0.150000 1.000 1.500 24 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 17 of 26

Attachment 2 Results of Acute DEEM-FCID Dietary Exposure Analysis

U.S. Environmental Protection Agency Ver. 2.02

DEEM-FCID ACUTE Analysis for ACIBENZOLAR-S-METHYL (1994-98 data)

Residue file: 061402AcuteConvertedMetaboliteWater.R98

 Adjustment factor #2 used.

Analysis Date: 07-19-2011/10:13:04 Residue file dated: 06-28-2011/07:37:37/8

Daily totals for food and foodform consumption used.

MC iterations = 1000 MC list in residue file MC seed = 10281

Run Comment: ""

===============================================================================

Summary calculations (per capita):

 95th Percentile 99th Percentile 99.9th Percentile

 Exposure % aRfD Exposure % aRfD Exposure % aRfD 

 ---------- -------- ---------- -------- ---------- --------

U.S. Population:

 0.001353 1.65 0.004014 4.90 0.014120 17.22 

All infants:

 0.002414 2.94 0.005284 6.44 0.013034 15.89 

Children 1-2 yrs:

 0.002618 3.19 0.008470 10.33 0.025051 30.55 

Children 3-5 yrs:

 0.002464 3.00 0.009451 11.53 0.028600 34.88 

Children 6-12 yrs:

 0.001409 1.72 0.005754 7.02 0.019931 24.31 

Youth 13-19 yrs:

 0.000985 1.20 0.003739 4.56 0.015067 18.37 

Adults 20-49 yrs:

 0.001095 1.33 0.003041 3.71 0.009085 11.08 

Adults 50+ yrs:

 0.001537 1.87 0.003851 4.70 0.010651 12.99 

Females 13-49 yrs:

 0.001169 1.43 0.003208 3.91 0.009561 11.66 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 18 of 26

Attachment 3 Chronic DEEM-FCID Residue Input File.

Filename: C:\Deem Files\061402 Acibenzolar\Acibenzolar on Low Growing Berries\061402Chronic.R98

Chemical: Acibenzolar-S-Methyl

RfD(Chronic): .082 mg/kg bw/day NOEL(Chronic): 0 mg/kg bw/day

RfD(Acute): .082 mg/kg bw/day NOEL(Acute): 0 mg/kg bw/day

Date created/last modified: 06-22-2011/09:35:41/8 Program ver. 2.03

--------------------------------------------------------------------------------

 EPA Crop Def Res Adj.Factors Comment

 Code Grp Commodity Name (ppm) #1 #2 

-------- ---- ------------------------------- ---------- ------ ------ -------

03002370 3 Onion, dry bulb 0.100000 1.000 1.500 

03002371 3 Onion, dry bulb-babyfood 0.100000 1.000 1.500 

03002380 3 Onion, dry bulb, dried 0.100000 9.000 1.500 

03002381 3 Onion, dry bulb, dried-babyfood 0.100000 9.000 1.500 

03002390 3 Onion, green 0.100000 1.000 1.500 

03003380 3 Shallot 0.100000 1.000 1.500 

04010050 4A Amaranth, leafy 0.250000 1.000 1.500 

04010180 4A Arugula 0.250000 1.000 1.500 

04011040 4A Chrysanthemum, garland 0.250000 1.000 1.500 

04011330 4A Cress, garden 0.250000 1.000 1.500 

04011340 4A Cress, upland 0.250000 1.000 1.500 

04011380 4A Dandelion, leaves 0.250000 1.000 1.500 

04011500 4A Endive 0.250000 1.000 1.500 

04012040 4A Lettuce, head 0.250000 1.000 1.500 

04012050 4A Lettuce, leaf 0.250000 1.000 1.500 

04012480 4A Parsley, leaves 0.250000 1.000 1.500 

04013130 4A Radicchio 0.250000 1.000 1.500 

04013550 4A Spinach 1.000000 1.000 1.500 

04013551 4A Spinach-babyfood 1.000000 1.000 1.500 

04020760 4B Cardoon 0.250000 1.000 1.500 

04020850 4B Celery 0.250000 1.000 1.500 

04020851 4B Celery-babyfood 0.250000 1.000 1.500 

04020860 4B Celery, juice 0.250000 1.000 1.500 

04020870 4B Celtuce 0.250000 1.000 1.500 

04021520 4B Fennel, Florence 0.250000 1.000 1.500 

04023220 4B Rhubarb 0.250000 1.000 1.500 

04023670 4B Swiss chard 0.250000 1.000 1.500 

05010610 5A Broccoli 1.000000 1.000 1.500 

05010611 5A Broccoli-babyfood 1.000000 1.000 1.500 

05010620 5A Broccoli, Chinese 1.000000 1.000 1.500 

05010640 5A Brussels sprouts 1.000000 1.000 1.500 

05010690 5A Cabbage 1.000000 1.000 1.500 

05010710 5A Cabbage, Chinese, napa 1.000000 1.000 1.500 

05010720 5A Cabbage, Chinese, mustard 1.000000 1.000 1.500 

05010830 5A Cauliflower 1.000000 1.000 1.500 

05011960 5A Kohlrabi 1.000000 1.000 1.500 

05020630 5B Broccoli raab 1.000000 1.000 1.500 

05020700 5B Cabbage, Chinese, bok choy 1.000000 1.000 1.500 

05021170 5B Collards 1.000000 1.000 1.500 

05021940 5B Kale 1.000000 1.000 1.500 

05022290 5B Mustard greens 1.000000 1.000 1.500 

05023180 5B Rape greens 1.000000 1.000 1.500 

05023890 5B Turnip, greens 1.000000 1.000 1.500 

08001480 8 Eggplant 1.000000 1.000 1.500 

08002340 8 Okra 1.000000 1.000 1.500 

08002700 8 Pepper, bell 1.000000 1.000 1.500 

08002701 8 Pepper, bell-babyfood 1.000000 1.000 1.500 

08002710 8 Pepper, bell, dried 1.000000 1.000 1.500 

08002711 8 Pepper, bell, dried-babyfood 1.000000 1.000 1.500 

08002720 8 Pepper, nonbell 1.000000 1.000 1.500 

08002721 8 Pepper, nonbell-babyfood 1.000000 1.000 1.500 

08002730 8 Pepper, nonbell, dried 1.000000 1.000 1.500 

08003740 8 Tomatillo 1.000000 1.000 1.500 

08003750 8 Tomato 1.000000 1.000 1.500 

08003751 8 Tomato-babyfood 1.000000 1.000 1.500 

08003760 8 Tomato, paste 3.000000 1.000 1.500 

08003761 8 Tomato, paste-babyfood 3.000000 1.000 1.500 

08003770 8 Tomato, puree 1.000000 2.900 1.500 

08003771 8 Tomato, puree-babyfood 1.000000 2.900 1.500 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 19 of 26

08003780 8 Tomato, dried 1.000000 14.300 1.500 

08003781 8 Tomato, dried-babyfood 1.000000 14.300 1.500 

08003790 8 Tomato, juice 1.000000 1.000 1.500 

09010750 9A Cantaloupe 2.000000 1.000 1.500 

09010800 9A Casaba 2.000000 1.000 1.500 

09011870 9A Honeydew melon 2.000000 1.000 1.500 

09013990 9A Watermelon 2.000000 1.000 1.500 

09014000 9A Watermelon, juice 2.000000 1.000 1.500 

09020210 9B Balsam pear 2.000000 1.000 1.500 

09020880 9B Chayote, fruit 2.000000 1.000 1.500 

09021020 9B Chinese waxgourd 2.000000 1.000 1.500 

09021350 9B Cucumber 2.000000 1.000 1.500 

09023080 9B Pumpkin 2.000000 1.000 1.500 

09023090 9B Pumpkin, seed 2.000000 1.000 1.500 

09023560 9B Squash, summer 2.000000 1.000 1.500 

09023561 9B Squash, summer-babyfood 2.000000 1.000 1.500 

09023570 9B Squash, winter 2.000000 1.000 1.500 

09023571 9B Squash, winter-babyfood 2.000000 1.000 1.500 

13020570 13B Blueberry 0.150000 1.000 1.500 

13020571 13B Blueberry-babyfood 0.150000 1.000 1.500 

86010000 O Water, direct, all sources 0.009460 1.000 1.000 

86020000 O Water, indirect, all sources 0.009460 1.000 1.000 

95000230 O Banana 0.100000 1.000 1.500 

95000231 O Banana-babyfood 0.100000 1.000 1.500 

95000240 O Banana, dried 0.100000 3.900 1.500 

95000241 O Banana, dried-babyfood 0.100000 3.900 1.500 

95001300 O Cranberry 0.150000 1.000 1.500 

95001301 O Cranberry-babyfood 0.150000 1.000 1.500 

95001310 O Cranberry, dried 0.150000 1.000 1.500 

95001320 O Cranberry, juice 0.150000 1.000 1.500 

95001321 O Cranberry, juice-babyfood 0.150000 1.000 1.500 

95002830 O Plantain 0.100000 1.000 1.500 

95002840 O Plantain, dried 0.100000 3.900 1.500 

95003590 O Strawberry 0.150000 1.000 1.500 

95003591 O Strawberry-babyfood 0.150000 1.000 1.500 

95003600 O Strawberry, juice 0.150000 1.000 1.500 

95003601 O Strawberry, juice-babyfood 0.150000 1.000 1.500 
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 20 of 26

Attachment 4 Results of Chronic DEEM-FCID Dietary Exposure Analysis.

U.S. Environmental Protection Agency Ver. 2.00

DEEM-FCID Chronic analysis for ACIBENZOLAR-S-METHYL (1994-98 data)

Residue file name: C:\Documents and Settings\tmorton\My Documents\MyFiles\DEEM Files\061402 

Acibenzolar\Acibenzolar on Low Growing Berries\061402Chronic.R98

 Adjustment factor #2 used.

Analysis Date 07-19-2011/11:14:22 Residue file dated: 06-24-2011/15:53:14/8

Reference dose (RfD, Chronic) = .082 mg/kg bw/day

===============================================================================

 Total exposure by population subgroup

-------------------------------------------------------------------------------

 Total Exposure

 -----------------------------------

 Population mg/kg Percent of 

 Subgroup body wt/day PAD 

-------------------------------------- ------------- ---------------

U.S. Population (total) 0.004366 5.3%

U.S. Population (spring season) 0.004431 5.4%

U.S. Population (summer season) 0.005515 6.7%

U.S. Population (autumn season) 0.003818 4.7%

U.S. Population (winter season) 0.003679 4.5%

Northeast region 0.004574 5.6%

Midwest region 0.004261 5.2%

Southern region 0.004000 4.9%

Western region 0.004871 5.9%

Hispanics 0.004983 6.1%

Non-hispanic whites 0.004220 5.1%

Non-hispanic blacks 0.004024 4.9%

Non-hisp/non-white/non-black 0.006247 7.6%

All infants (< 1 year) (.082*) 0.003808 4.6%

Nursing infants (.082*) 0.002360 2.9%

Non-nursing infants (.082*) 0.004358 5.3%

Children 1-6 yrs (.082*) 0.008690 10.6%

Children 7-12 yrs (.082*) 0.005490 6.7%

Females 13-19 (not preg or nursing) (.082*)

 0.003654 4.5%

Females 20+ (not preg or nursing) (.082*) 0.003757 4.6%

Females 13-50 yrs (.082*) 0.003897 4.8%

Females 13+ (preg/not nursing) (.082*) 0.003521 4.3%

Females 13+ (nursing) (.082*) 0.004935 6.0%

Males 13-19 yrs (.25*) 0.004129 1.7%

Males 20+ yrs (.25*) 0.003680 1.5%

Seniors 55+ (.25*) 0.003886 1.6%

Children 1-2 yrs (.082*) 0.009207 11.2%

Children 3-5 yrs (.082*) 0.008706 10.6%

Children 6-12 yrs (.082*) 0.005812 7.1%

Youth 13-19 yrs (.082*) 0.003893 4.7%

Adults 20-49 yrs (.25*) 0.003654 1.5%

Adults 50+ yrs (.25*) 0.003852 1.5%

Females 13-49 yrs (.082*) 0.003646 4.4%

*PAD in mg/kg-bw-day

-------------------------------------------------------------------------------
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 21 of 26

Attachment 5 Percent Crop Treated Estimates Provided by BEAD
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 22 of 26
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 23 of 26
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 24 of 26

6. RDF Files Used in Acute Analysis

RDF #1

Banana

%CT = 100

TOTALNZ = 30

TOTALZ = 0

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.01

0.02

0.04

0.03

0.04

0.07

0.02

0.04

0.05

RDF #2

Bell Pepper

% CT= 10

TOTALNZ=9

TOTALZ=81

0.16

0.28

0.34

0.40

0.53

0.29

0.27

0.30

0.32

RDF #3

Broccoli

% CT= 5

TOTALNZ=11

TOTALZ=209

0.58

0.28

0.34

0.42

0.52

0.17

0.22

0.32

0.60

0.60

0.63

RDF #4

Cabbage

% CT= 1

TOTALNZ=11

TOTALZ=1089

0.57

0.19

0.22

0.12

0.14

0.28

0.36

0.06

0.09

0.27

0.35

RDF #5

Celery

% CT= 100

TOTALNZ=9

TOTALZ=0

0.045

0.018

0.016

0.050

0.043

0.058

0.042

0.077

0.063

RDF #6

Head Lettuce 

% CT= 15

TOTALNZ=12

TOTALZ=68

0.081

0.075

0.049

0.074

0.033

0.041

0.044

0.039

0.105

0.093

0.043

0.055

RDF #7

Hot Pepper

% CT= 10

TOTALNZ=5

TOTALZ=45

0.50

0.41

0.35

0.21

0.32

RDF #8

Leaf Lettuce

% CT= 15

TOTALNZ=11

TOTALZ=62

0.099

0.151

0.136

0.073

0.035

0.112

0.183

0.185

0.175

0.037

0.044

RDF #9

Mustard Greens

% CT= 100

TOTALNZ=9

TOTALZ=0

0.74

0.53

0.64

0.71

0.80

0.26

0.32

0.14

0.18

RDF #10

Spinach

% CT= 40

TOTALNZ=11

TOTALZ=17

0.273

0.326

0.247

0.418

0.538

0.110

0.138

0.232

0.262

0.230

0.190
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 25 of 26

RDF #11

Tomato

% CT= 5

TOTALNZ=23

TOTALZ=437

0.13

0.21

0.21

0.06

0.08

0.46

0.25

0.11

0.16

0.09

0.09

0.09

0.09

0.06

0.08

0.14

0.08

0.16

0.20

0.22

0.32

0.22

0.27

RDF #12

BellPepperOth

% CT= 100

TOTALNZ=9

TOTALZ=0

0.16

0.28

0.34

0.40

0.53

0.29

0.27

0.30

0.32

RDF #13

BroccoliOth

% CT= 100

TOTALNZ=11

TOTALZ=0

0.58

0.28

0.34

0.42

0.52

0.17

0.22

0.32

0.60

0.60

0.63

RDF #14

CabbageOth

% CT= 100

TOTALNZ=11

TOTALZ=0

0.57

0.19

0.22

0.12

0.14

0.28

0.36

0.06

0.09

0.27

0.35

RDF #15

CeleryOth

% CT= 100

TOTALNZ=9

TOTALZ=0

0.045

0.018

0.016

0.050

0.043

0.058

0.042

0.077

0.063

RDF #16

LettuceHRad 

% CT= 100

TOTALNZ=12

TOTALZ=0

0.081

0.075

0.049

0.074

0.033

0.041

0.044

0.039

0.105

0.093

0.043

0.055

RDF #17

HotPepperOth

% CT= 100

TOTALNZ=5

TOTALZ=0

0.50

0.41

0.35

0.21

0.32

RDF #18

SpinachOth

% CT= 100

TOTALNZ=11

TOTALZ=0

0.273

0.326

0.247

0.418

0.538

0.110

0.138

0.232

0.262

0.230

0.190

RDF #19

TomatoOth

% CT= 100

TOTALNZ=23

TOTALZ=0

0.13

0.21

0.21

0.06

0.08

0.46

0.25

0.11

0.16

0.09

0.09

0.09

0.09

0.06

0.08

0.14

0.08

0.16

0.20

0.22

0.32

0.22

0.27

RDF #20

Cantaloupe

% CT= 100

TOTALNZ=6

TOTALZ=0

0.235

0.759

0.240

0.279

0.278

0.134
Acibenzolar-S-Methyl (PC Code 061402) Dietary Exposure and Risk Assessment DP #: 387069

Page 26 of 26

RDF #21

Cucumber

% CT= 100

TOTALNZ=8

TOTALZ=0

0.166

0.508

0.183

0.210

0.218

0.215

0.155

0.132

RDF #22

Squash

% CT= 100

TOTALNZ=5

TOTALZ=0

0.152

0.418

0.151

0.187

0.172

RDF #23

Onion 

% CT= 100

TOTALNZ=7

TOTALZ=0

0.025

0.025

0.025

0.056

0.025

0.025

0.025

RDF #24

Strawberry

%CT=100

TOTALNZ=20

TOTALZ=0

0.020

0.032

0.035

0.023

0.050

0.040

0.067

0.058

0.064

0.062

0.040

0.038

0.076

0.054

0.083

0.087

0.020

0.022

0.023

0.025

RDF #25

Cauliflower

% CT= 10

TOTALNZ=11

TOTALZ=99

0.58

0.28

0.34

0.42

0.52

0.17

0.22

0.32

0.60

0.60

0.63