MLB Summary of Disinfectant Products Tested via the OECD Quantitative Method

A Hydrogen peroxide Liquid 0.5 H * RTU NT 1 min. Yes N/A 5 6.6E+06 2 2.6E+06 3.0 ≥ 5.9 5 min. N/A 0.500 Y 

B Phenol Liquid 19 H * 1 + 255 NT 10 min. Yes 400 ppm hard water 2 5.3E+06 3 1.4E+07 6.0 5.5 5 min. 375 ppm hard water 0.074 Y 

C Phenol Liquid 22.5 H * 1 + 255 NT 10 min. Yes 500 ppm hard water 35 1.4E+07 16 7.9E+06 ≥ 6.8 ≥ 5.7 5 min. 375 ppm hard water 0.088 Y 

D Phenol Liquid 16.75 H * 1 + 127 NT 10 min. Yes 400 ppm hard water 0 1.6E+06 1 1.9E+06 ≥ 6.7 ≥ 5.9 5 min. 375 ppm hard water 0.131 Y 

E Quat Liquid 3.85 H * 3 + 125 NT 10 min. Yes 400 ppm hard water 0 1.2E+07 5 8.6E+06 4.6 0.6 5 min. 375 ppm hard water 0.090 Y 

F Quat Liquid 16.9 H * 1 + 255 NT 10 min. Yes 400 ppm hard water 0 3.8E+06 7 9.1E+06 4.1 ≤ 0.9 5 min. 375 ppm hard water 0.066 Y 

G Quat Liquid 2.113 H * 1 + 31 NT 10 min. Yes 400 ppm hard water 0 6.5E+06 48 1.1E+07 4.1 ≤ 0.9 5 min. 375 ppm hard water 0.066 Y 

H Quat Liquid 3.85 H * 1 + 63 NT 10 min. Yes 250 ppm hard water 0 4.6E+06 4 7.6E+06 6.3 ≤ 0.8 5 min. 375 ppm hard water 0.060 Y 

I Quat Liquid 1.1856 S * NT 1 + 15 1 min. No DI water N/A N/A N/A N/A ≤ 1.7 ≤ 0.9 5 min. 375 ppm hard water 0.074 Y 

J Quat + HCl Liquid 0.05 + 9 H * 1 + 2 NT 10 min. Yes Tap water 0 5.1E+06 0 4.1E+06 ≥ 6.9 ≥ 5.8 5 min. 375 ppm hard water 0.017 + 3 Y 

K Sodium hypochlorite Liquid 6 H * 1 + 13.8 NT 5 min. Yes DI water 2 9.3E+06 2 2.4E+06 ≥ 6.8 ≥ 5.7 5 min. 375 ppm hard water 0.405 Y 

L Sodium hypochlorite Liquid 6.15 H * 5.33 + 128 NT 5 min. No DI water 0 8.3E+06 0 2.9E+06 ≥ 6.7 ≥ 5.9 5 min. 375 ppm hard water 0.246 Y 

M Citric acid Liquid 0.6 H * RTU NT 5 min. Yes N/A 1 9.8E+06 0 6.1E+06 ≥ 6.7 ≥ 5.9 5 min. N/A 0.600 Y 

The OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides (OECD method) recently underwent a multi-laboratory ring trial and the EPA is interested in adopting the OECD method for regulatory purposes. As part of the implementation procreduction values of currently registered liquid antimicrobial products using the OECD procedure. Over the course of this study, 13 different products were evaluated against both Staphylococcus aureus and Pseudomonas aeruginosa using parameters specified for the UD

All use-dilutions were based on product label claims. For the purposes of comparing products with 2 sets of claims, a side-by-side comparative evaluation was conducted (the order of the parameters tested within a test day was randomized). Products were evaluated fir

OECD TEST METHO

Contact 

time (min) Diluent

Carrier 

Counts 

(S.a.)

Efficacy 

(P.a.) 

Positive 

carriers

Carrier 

Counts 

(P.a.)

3 Part 

OSL 

used? 

(Y or N)

UseDilution

Label Claim 

Log 

Reduction 

S.a.**

Label Claim 

Log 

Reduction 

P.a.**

ATP RESULTS

Efficacy 

(S.a.) 

Positive 

carriers

OECD TEST METHOD 

RESULTS -- LABEL 

CLAIM PARAMETERS

PRODUCT EFFICACY USE DIRECTIONS ATP PARAMETERS

Product 

ID

Product 

Format

Product 

Efficacy 

Claim (as 

tested)

Product 

Use 

Directions

Chemical 

Classification of 

Product 

Active(s)

Percent 

Active(s) 

declared on 

the label

Use-dilution 

for 

Sanitization

Use-Dilution 

for 

Disinfection

Diluent

Contact 

Time 

(min)

OSL 

(Y/N)

*Parameters are developed using the product label, original studies, and the master label.**Values listed as ≥ represent complete kill. Values listed as ≤ represent a value of 1000 that was substituted at the least concentrated dilution because growth on the filter was a lawn.Product ID - corresponds to product identifical list as provided by EPAChemical Classifivation of Product Active(s) - corresponds to the product active as declared on the label. This could be one active (Quaternary, Citric Acid, Phenolic)Product Format - this corresponds to the format the product is being sold in. This could be a concentrated liquid dilutable, a trigger spray, pump spray or aerosol spray product for examplePercent Active(s) declared on the label - this is the percentage of the Active(s) as declared on the product labelProduct Efficacy Claim (as tested) - this corresponds to the product efficacy category that was tested. This includes Hospital Grade Disinfectants (H) and Sanitizers (S)Product Use Directions - this corresponds to where the use directions were derived from including the product graphic Label (L) or Master Label (ML)Use-dilution for Disinfection - this corresponds to the use-directions on how to apply the product for use as a disinfectant. For example Ready to Use (RTU) or product dilution instructions. NT = not testedUse-dilution for Sanitization - this corresponds to the use-directions on how to apply the product for use as a sanitizer. For example Ready to Use (RTU) or product dilution instructions. NT = not testedContact Time (min) - this corresponds to the contact time the product needs for that efficacy category.OSL (Y/N) - this corresponds to the use of an organic soil load in the Use-Dilution testDiluent - this corresponds to the diluent used in the Use-Dilution testATP Results - Efficacy (S.a.) - this corresponds to the efficacy data determined through ATP testing. Value given is the number of positive carriers out of 60.ATP Results - Carrier Count (S.a.) - this corresponds to the average dried carrier count data. For example 1.0 x 10^6 CFU/mLATP Results - Efficacy (P.a.) - this corresponds to the efficay data determination through ATP testing. Value given is the number of positive carriers out of 60.ATP Results - Carrier Counts (P.a.) - this corresponds to the average dried carrier count data. For example 1.0 x 10^6 CFU/mLOECD Test Method Results -- Label Claim Parameters - Label Claim Log Reduction S.a. - this corresponds to the log reduction when tested at the label claim for this organismOECD Test Method Results -- Label Claim Parameters - Label Claim Log Reduction P.a. - this corresponds to the log reduction when tested at the label claim for this organismContact Time (min) - this corresponds to the contact time product where product efficacy testing was performedDiluent - this corresponds to the diluent used for product dilution for efficacy testingUse-dilution - this corresponds to the final product concentration during testing3 Part OSL (organic soil load) Used - this corresponds to whether or not the 3 part organic soil in the method was used in the testingOECD Parameters Log Reduction S.a. - this corresponds to the log reduction when tested with the OECD parameters for this organismOECD Parameters Log Reduction P.a. - this corresponds to the log reduction when tested with the OECD parameters for this organismControl Carrier Count (S.a.) - this corresponds to the dried carrier control count for this organismControl Carrier Count (P.a.) - this corresponds to the the dried carrier control count for this organism
* *

†

* * * * *

† †

†

* * * *

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

A B C D E F G H I J K L M

Log10 Reduction

S. aureus Log Reduction using Label Claim

and OECD Test Parameters

Label Claim

OECD

Hydrogen 

peroxide

Phenol Sodium 

hypochlorite

Quaternary ammonium 

compounds

Quat + 

HCl

Citric 

acid

Test Chemicals

* = Value listed represents complete kill. 

† = Value listed represents a value of 1,000 that was substituted at the least concentrated dilution because growth on the filter was a lawn.
* * *

† † † †

* * * *

* *

† † † †

†

* * * *

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

A B C D E F G H I J K L M

Log10 Reduction

P. aeruginosa Log Reduction using Label Claim

and OECD Test Parameters

Label Claim

OECD

Hydrogen 

peroxide

Phenol Sodium 

hypochlorite

Quaternary ammonium 

compounds

Quat + 

HCl

Citric 

acid

Test Chemicals

* = Value listed represents complete kill. 

† = Value listed represents a value of 1,000 that was substituted at the least concentrated dilution because growth on the filter was a lawn.