## Effluent Quality Assessment 

# Florida Power and Light Company St. Lucie Plant

St. Lucie County

NPDES #FL0002208
Sampled April 5, 2010

June 2010

Biology Section
Bureau of Laboratories
Division of Environmental Assessment and Restoration

Quality Manual No. 870346G
NELAC Certification No. E31780

### 1 CONTENTS

Introduction

Methods

Results and Discussion

Literature Cited

Tables Table 1 Measured algal growth potential (AGP) Table 2 Effluent limits, Class III Marine Criteria and chemical data

Appendices Appendix A Chain of Custody Appendix B Bioassay Bench Sheets Appendix C SRT Bench Sheet s and Statistical Analyses Appendix D Organism Receipt Appendix E Chemical Analyses of Effluent Appendix F Facility Summary, Effl uent Limits, and Permit Violations Recorded on Discharge Monitoring Report Appendix G EPA End Sheet

### 2 Florida Power & Light Company – St. Lucie Plan t, 6501 S. Ocean Drive, Jensen Beach, St. Lucie County, Florida, 34957. NPDES #FL0002208. Effluent grab samples for bioassay and chemical analyses for this facility were collected on April 5, 2010.



### Introduction Florida Power & Light Company – St. Lucie Plant is a nuclear power plant which treats industrial wastewater through neutralization, settling, i on exchange, and microfiltration. (See Appendix F for detailed facility information provided by Terry Davis of FDEP Southeast District.)



### Methods Samples were collected from the EFF-2 NPDES sa mpling point for Outfall D-001 by Terry Davis and Danielle Melnick (FDEP Southeas t District) according to permit requirements. Two five-gallon portions of the once-through cooling water (OTCW) were obtained from the discharge from Unit #2 and 8 mL of a chemical solution consisting of 30 ppm ETA, 25 ppm N 2 

### H 4 

### , and 20 ppm CH 2 

### was added to each to simulate normal discharge. The samples for all analyses were taken from the two five-gallon portions.

See Appendix A for the completed chain of custody form.

The 96-hour acute screening toxicity tests and algal growth potential test were performed following internal DEP SOPs TA07_03, TA07_04, and TA08_01 through TA08_09. All internal DEP SOPs are available on the web at: http:// www.floridadep.org/labs/cgi-bin/sop/biosop.asp

.

The salinity of the sample was adjusted from an init ial salinity of 36.4 ppt to a final salinity of 34.9 ppt by the addition of deionized water, in or der to conform to the method requirements for saltwater test species.



### Results and Discussion

Toxicity Test Results Tests were performed April 6 -10, 2010.

Americamysis bahia 96-hour acute screening bioassay – LC 50 

### > 100%, 5%* mortality in 100% sample at 96 hours

Menidia beryllina 96-hour acute screening bioassay – LC 50 

### > 100%, 0%* mortality in 100% sample at 96 hours

The bioassay samples were not acutely toxic to the test organisms.

*NOTE: In a 96-hour acute screening test of 100% effluent, mortality of <20% provides
confidence that the effluent meets the acute to xicity criterion of Florida’s Surface Water Quality Standards (Rule 62-302.500 (1) (a) 4, F. A.C.). Mortality between 20-50% indicates low to moderate levels of toxicity, and furt her action may be required. Mortality of >50%
indicates that the effluent fails to meet the mi nimum requirement to discharge to waters of the state (Rule 62-4.244(3) (a), F.A.C.).

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard reference toxicant (SRT) tests and the bench sheets and statistical analyses of the SRT tests that correspond to these facility te sts. See Appendix D for test organism receipts.

### 3 Algal Growth Potential The effluent Algal Growth Potential (AGP) was 3.0 U (U - Material was analyzed for but not detected; the value reported is the minimum det ection limit) mg dry wt/L of the saltwater species Dunaliella tertiolecta. Raschke and Schultz found that AGP values above 10.0 mg dry weight/L represent a “problem” threshold for marine receiving waters, implying nutrient enrichment (personal communication, Marshall Fa ircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). The ratio (17.0:1) of to tal nitrogen to total pho sphorus sugge sts that the effluent is co-limited by both nitrogen and phosphorus. The ratio (2.9:1) of inorganic nitrogen and orthophosphate suggest that the effluent is nitrogen limited (Maloney et al. 1978).

See Table 1 for AGP results.

Chemistry Results Total residual chlorine was not detected, and total ammonia was not measured in the bioassay sample in the laboratory. The total ammonia concentration in the composite sample collected and preserved for chemical analysis was 0.022 mg N/L. Based on the pH, salinity, and temperature of the effluent as collected, the calculated unionized ammonia concentration was < 0.02 mg/L.

Aluminum was detected in the effluent at leve ls that comply with Class III marine water quality criteria (62-302.530, F.A.C.). Ars enic was detected between the laboratory method detection limits (MDL) and practical quantitation limits (PQL). Oils and Greases were detected in the equipment blank, but not in the effluent.

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See Appendix E for a complete list of chemical analyses performed.



### Conclusion The effluent sample collected from the EFF-2 NPDES sampling point for Outfall D-001 for this facility on April 5, 2010, was not acutely toxic to either test species during the 96-hour acute screening bioassays. The effluent AGP result wa s less than the "problem” threshold for marine receiving waters. Effluent water quality sample s collected on April 5, 2010, did not exceed any applicable water quality standards or violate any permit conditions.



### Literature Cited Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: Bottle Test. National Environmental Research Center Office of Research and Development. U. S. EPA, Corvallis, Oregon. 43 p.

### 4 Inor g anic Total

Location N:P ratio N:P ratio

D-001 3.0 U 2.9 17.0 U - Material analyzed for but not detected; value reported is the method detection limit Table 1. Measured algal growth potential (AGP; mg dry weight/L) of the saltwater species Dunaliella tertiolecta and ratios of nitrogen to phosphorus for samples collected from the FPL - St. Lucie Plant Outfall on April 5,

2010.

AGP FPL Co - St. Lucie Plant
NPDES# FL0002208

(measured)

### 5 FPL Co - St. Lucie Plant

NPDES# FL0002208 Class III

Criteria Effluent

Limits

Aluminum ≤ 1500 - 1400

Arsenic ≤ 50 - 1.6 I

Cadmium ≤ 8.8 - 0.09 U Calcium (mg/L) - - 421

Chromium-III - - 0.9 U Copper ≤ 3.7 - 1.5 U

Iron ≤ 300 - 90 U

Lead ≤ 8.5 - 0.6 U Magnesium (mg/L) - - 1380

Nickel ≤ 8.3 - 0.75 U

Selenium ≤ 71 - 0.6 U

Silver ≤ 2.3 - 0.075 U

Zinc ≤ 86 - 15 U

Ortho-phosphate - - 0.013 I Total Phosphorus - - 0.021

Total Ammonia - - 0.022

Un-ionized Ammonia - - ≤ 0.02 c

Nitrate and Nitrite - - 0.016 Total Kjeldahl Nitrogen - - 0.34 I Organic Nitrogen - - 0.318 c Total Nitrogen - - 0.356 c

Dissolved Oxygen (mg/L) ≥ 4.0 - 6.6 £ pH (SU) 6.5 - 8.5 - 7.7 Conductivity (μmhos/cm) - - 55580 Temperature (°C) - ≤ 45 34.3 Salinity - - 30.4 c Total Residual Chlorine (mg/L) ≤ 0.01 - ≤ 0.03 UQ£ Chlorophyll a (μg/L) - Corrected - - 0.55 U Phaeophytin (μg/L) - - 0.4 U Total Suspended Solids (mg/L) - - 13 I Oils and Greases (mg/L) ≤ 5.0 - 1.4 UF Flow (MGD) - Report 1,487 a Hardness (mg CaCO 3 ) - - 6734.08 c

Value exceeds the Class III Water Quality Criteria or permit limits a - Annual average

c - Value is calculated F - Analyte was detected in the equipment blank (1.5 I mg/L) I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit U - Material analyzed for but not detected; value reported is the method detection limit Q - Sample held beyond normal holding time £ - Measured in Central Laboratory Toxicity lab General Physical and Chemical Parameters Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical data for samples collected from the Florida Power & Light Company - St. Lucie Plant on April 5, 2010.

D-001

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)

### 6 Appendix A. Chain of Custody
Form

### 7 Appendix B. Bench Sheets

### 8 Appendix B. (continued)

### 9 Appendix B. (continued)

### 10 Appendix B. (continued)

### 11 Appendix B. (continued)

### 12 Appendix B. (continued)

### 13 Appendix C. SRT Bench Sheet s and Statistical Analysis Florida Department of Environmental Protection
Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 96-hour Acute Toxicity Americamysis bahia (mysid shrimp) - Cadmium Chloride (CdCl 2 

### )

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit
10/07/08 21.89 27.66 4.73 18.21 37.11
11/12/08 25.50 27.40 4.69 18.01 36.78
12/02/08 27.31 27.13 4.53 18.07 36.20
01/06/09 31.20 26.79 3.86 19.07 34.50
02/03/09 25.44 26.56 3.79 18.97 34.15
03/03/09 24.49 26.78 3.52 19.75 33.81
04/08/09 24.49 26.45 3.39 19.66 33.23
05/05/09 27.98 26.39 3.36 19.68 33.10
06/09/09 22.89 26.09 3.39 19.32 32.87
07/07/09 30.00 26.43 3.43 19.57 33.28
08/04/09 26.14 26.65 3.24 20.16 33.13
09/01/09 22.89 26.69 3.19 20.30 33.07
09/29/09 22.46 26.17 2.97 20.22 32.11
11/03/09 23.12 26.08 3.04 20.00 32.15
12/1/09 31.91 26.03 2.94 20.16 31.91
1/5/10 25.00 25.91 2.92 20.06 31.75
2/2/10 26.18 25.77 2.84 20.09 31.46
2/9/10 41.45 26.68 4.46 17.76 35.59
3/3/10 28.31 26.78 4.47 17.84 35.72
4/6/10 24.49 26.66 4.50 17.66 35.65

CV = 16.88 Lower Confidence Limit = mean - 2 * Std Dev EPA reference 75th quartile CV = 26% Upper Confidence Limit = mean + 2 * Std Dev

10 15 20 25 30 35 40 45 5010/07/0811/12/0812/02/0801/06/0902/03/0903/03/0904/08/0905/05/0906/09/0907/07/0908/04/0909/01/0909/29/0911/03/0912/1/091/5/102/2/102/9/103/3/104/6/10μg/L CdCl2 

### Standard Reference Toxicant Data Americamysis bahia 96 hour LC 50

LC50 Mean LC50 Lower Control Limit Upper Control Limit

### 14 Appendix C. (continued) Florida Department of Environmental Protection
Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 96-hour Acute Toxicity Menidia beryllina (inland silverside) - Copper Chloride (CuCl 2 

### ) as Cu

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit
11/04/08 249.81 305.57 62.50 180.57 430.57
12/02/08 316.79 308.13 61.82 184.48 431.78
01/06/09 253.83 308.45 61.51 185.44 431.46
02/03/09 245.51 304.15 62.80 178.55 429.74
03/03/09 416.64 307.01 66.63 173.74 440.27
03/31/09 228.94 300.89 67.96 164.97 436.80
05/05/09 326.07 300.24 67.64 164.97 435.52
06/02/09 465.39 302.54 72.50 157.54 447.53
06/09/09 355.67 302.52 72.48 157.55 447.48
07/07/09 403.35 307.19 75.91 155.37 459.02
08/04/09 343.29 306.49 75.48 155.52 457.45
09/09/09 402.57 311.84 78.40 155.03 468.65
09/29/09 497.5 318.42 88.10 142.21 494.62
10/13/09 426.5 326.12 90.57 144.97 502.27
11/3/09 441.06 329.60 93.69 142.22 516.99
12/1/09 390.28 335.59 93.54 148.51 522.68
1/5/10 387.66 343.92 90.17 163.57 524.26
2/9/10 306.1 350.85 80.73 189.39 512.30
3/3/10 298.9 353.79 77.48 198.82 508.76
3/30/10 333.83 354.48 77.23 200.03 508.94

CV = 21.79 EPA reference 75th quartile CV = 21%

0 100 200 300 400 500 60011/04/0812/02/0801/06/0902/03/0903/03/0903/31/0905/05/0906/02/0906/09/0907/07/0908/04/0909/09/0909/29/0910/13/0911/3/0912/1/091/5/102/9/103/3/103/30/10μg/L Cu



### Test Date 

### Standard Reference Toxicant Data Menidia beryllina 96-hour LC 50

LC50 Mean LC50 Lower Control Limit Upper Control Limit

### 15 Appendix C. (continued)

### 16 Appendix C. (continued)

### 17 Appendix C. (continued)

### 18 Appendix C. (continued)

### 19 Appendix C. (continued)

A mericamysis bahia Acute Standard Reference Toxicant Test

DATE: 4

‐ 6

‐ 10 DURATION: 96 hr TOXICANT: CdCl 2 SPECIES: Americamysis bahia

RAW DATA: Concentration Number Mortalities (μg/L) Exposed

.00 20 0

20.00 20 5

30.00 20 15

45.00 20 18

67.00 20 20

100.00 20 20

SPEARMAN

‐ KARBER TRIM: 25.00%

SPEARMAN

‐ KARBER ESTIMATES: LC50: 24.49

95% LOWER CONFIDENCE: 21.92

95% UPPER CONFIDENCE: 27.37

Menidia beryllina Acute Standa rd Re ference Toxicant Test

DATE: 3

‐ 30

‐ 10 DURATION: 96 hr TOXICANT: CuCl 2 SPECIES: Menidia beryllina

RAW DATA: Concentration Number Mortalities (μg/L) Exposed

.00 20 1

100.00 20 0

200.00 20 1

300.00 20 4

400.00 20 17

500.00 20 20

SPEARMAN

‐ KARBER TRIM: 0%

SPEARMAN

‐ KARBER ESTIMATES: LC50: 333.83

95% LOWER CONFIDENCE: 307.51

95% UPPER CONFIDENCE: 362.40

NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING. ADJUSTMENTS WERE

MADE PRIOR TO SPEARMAN

‐ KARBER ESTIMATION.

### 20 Appendix D. Test Organism Receipts

### 21 Appendix D. (continued)22Appendix E. Chemical Analyses performed on the effluent from the Florida Power & Light – St. Lucie Plant OutfallD-001, sampled on April 5, 2010.Date Sampled Field ID Analysis Group Components Result Remark Units MDL PQL4/5/2010 12:20 D-001 AGP/LN Algal Growth Potential 3 U mg DryWt/L 3 34/5/2010 12:20 D-001 Chlorophyll/Grain Size/BOD Phaeophytin-a 0.4 U ug/L 0.4 1.24/5/2010 12:20 D-001 Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 0.55 U ug/L 0.55 1.74/5/2010 12:20 D-001 Toxicology BSY-AS-SW-Mysid, LC50 100 L LC504/5/2010 12:20 D-001 Toxicology BSY-AS-SW-Fish, LC50 100 L LC504/5/2010 12:20 D-001 Metals Selenium 0.6 U ug/L 0.6 2.44/5/2010 12:20 D-001 Metals Nickel 0.75 U ug/L 0.75 34/5/2010 12:20 D-001 Metals Magnesium 1380 mg/L 2 84/5/2010 12:20 D-001 Metals Cadmium 0.09 U ug/L 0.09 0.364/5/2010 12:20 D-001 Metals Aluminum 1400 ug/L 180 7204/5/2010 12:20 D-001 Metals Iron 90 U ug/L 90 3604/5/2010 12:20 D-001 Metals Chromium 0.9 U ug/L 0.9 3.64/5/2010 12:20 D-001 Metals Zinc 15 U ug/L 15 604/5/2010 12:20 D-001 Metals Calcium 421 mg/L 0.22 0.94/5/2010 12:20 D-001 Metals Silver 0.075 U ug/L 0.075 0.34/5/2010 12:20 D-001 Metals Arsenic 1.6 I ug/L 0.75 34/5/2010 12:20 D-001 Metals Copper 1.5 U ug/L 1.5 34/5/2010 12:20 D-001 Metals Lead 0.6 U ug/L 0.6 2.44/5/2010 12:20 D-001 Nutrients Kjeldahl Nitrogen 0.34 I mg N/L 0.16 0.44/5/2010 12:20 D-001 Nutrients Total-P 0.021 mg P/L 0.008 0.024/5/2010 12:20 D-001 Nutrients Ammonia-N 0.022 mg N/L 0.01 0.024/5/2010 12:20 D-001 Nutrients NO2NO3-N 0.016 mg N/L 0.004 0.014/5/2010 12:20 D-001 Nutrients O-Phosphate-P 0.013 I mg P/L 0.008 0.024/5/2010 12:20 D-001 Nutrients TSS 13 I mg/L 4 164/5/2010 12:20 D-001 Overflow Oil and Grease 1.4 U mg/L 1.4 54/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(a)anthracene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Diethyl phthalate 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(k)fluoranthene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Dibenzo(a,h)anthracene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Hexachlorobenzene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Hexachloroethane 2.8 U ug/L 2.8 1123Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remark Units MDL PQL4/5/2010 12:20 D-001 Priority Organic Pollutants Naphthalene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 2-Chlorophenol 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 14 564/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(b)fluoranthene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Phenanthrene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 2,4,6-Trichlorophenol 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 14 564/5/2010 12:20 D-001 Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.8 U ug/L 2.8 114/5/2010 12:20 D-001 Priority Organic Pollutants 4-Chloro-3-methylphenol 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic PollutantsBis(2-ethylhexyl)phthalate 14 UJ ug/L 14 564/5/2010 12:20 D-001 Priority Organic Pollutants Dimethyl phthalate 9.3 U ug/L 9.3 374/5/2010 12:20 D-001 Priority Organic Pollutants Pentachlorophenol 2.8 U ug/L 2.8 114/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dinitrotoluene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 3,3'-Dichlorobenzidine 37 U ug/L 37 1504/5/2010 12:20 D-001 Priority Organic Pollutants Acenaphthylene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Fluoranthene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Fluorene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Hexachlorobutadiene 2.8 U ug/L 2.8 114/5/2010 12:20 D-001 Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 7.44/5/2010 12:20 D-001 Priority Organic Pollutants Pyrene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(a)pyrene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Benzo(g,h,i)perylene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic PollutantsBis(2-chloroethoxy)methane 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.8 U ug/L 2.8 114/5/2010 12:20 D-001 Priority Organic Pollutants Butyl benzyl phthalate 4.7 U ug/L 4.7 194/5/2010 12:20 D-001 Priority Organic Pollutants Di-n-butyl phthalate 4.7 U ug/L 4.7 194/5/2010 12:20 D-001 Priority Organic Pollutants Di-n-octyl phthalate 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.8 U ug/L 2.8 114/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dichlorophenol 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 2,4-Dimethylphenol 9.3 U ug/L 9.3 374/5/2010 12:20 D-001 Priority Organic Pollutants 2-Chloronaphthalene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Benzidine 93 U ug/L 93 37024Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remark Units MDL PQL4/5/2010 12:20 D-001 Priority Organic PollutantsBis(2-chloroethyl)ether 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 7.44/5/2010 12:20 D-001 Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 7.44/5/2010 12:20 D-001 Priority Organic Pollutants Phenol 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 2,6-Dinitrotoluene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants 2-Nitrophenol 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 7.44/5/2010 12:20 D-001 Priority Organic Pollutants Acenaphthene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Anthracene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Chrysene 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Priority Organic Pollutants Hexachlorocyclopentadiene 2.8 U ug/L 2.8 114/5/2010 12:20 D-001 Priority Organic Pollutants Isophorone 0.93 U ug/L 0.93 3.74/5/2010 12:20 D-001 Field Parameter Temperature 34.34 C4/5/2010 12:20 D-001 Field Parameter Sample Depth 0.1 m4/5/2010 12:20 D-001 Field Parameter pH 7.74/5/2010 12:20 D-001 Field Parameter Specific Conductance 55580 umhos/cm4/5/2010 12:10 Blank Metals Aluminum 60 U ug/L 60 2404/5/2010 12:10 Blank Metals Cadmium 0.03 U ug/L 0.03 0.124/5/2010 12:10 Blank Metals Nickel 0.25 U ug/L 0.25 14/5/2010 12:10 Blank Metals Arsenic 0.25 U ug/L 0.25 14/5/2010 12:10 Blank Metals Zinc 5 U ug/L 5 204/5/2010 12:10 Blank Metals Magnesium 0.04 U mg/L 0.04 0.164/5/2010 12:10 Blank Metals Chromium 0.3 U ug/L 0.3 1.24/5/2010 12:10 Blank Metals Lead 0.2 U ug/L 0.2 0.84/5/2010 12:10 Blank Metals Copper 0.5 U ug/L 0.5 14/5/2010 12:10 Blank Metals Silver 0.025 U ug/L 0.025 0.14/5/2010 12:10 Blank Metals Calcium 0.075 U mg/L 0.075 0.34/5/2010 12:10 Blank Metals Selenium 0.2 U ug/L 0.2 0.84/5/2010 12:10 Blank Metals Iron 30 U ug/L 30 1204/5/2010 12:10 Blank Nutrients Ammonia-N 0.01 U mg N/L 0.01 0.024/5/2010 12:10 Blank Nutrients O-Phosphate-P 0.004 U mg P/L 0.004 0.014/5/2010 12:10 Blank Nutrients NO2NO3-N 0.012 mg N/L 0.004 0.014/5/2010 12:10 Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 0.08 0.225Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remark Units MDL PQL4/5/2010 12:10 Blank Nutrients Total-P 0.004 U mg P/L 0.004 0.014/5/2010 12:10 Blank Overflow Oil and Grease 1.5 I mg/L 1.4 54/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dimethylphenol 9.7 U ug/L 9.7 394/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dinitrophenol 15 U ug/L 15 584/5/2010 12:10 Blank Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants Benzo(a)pyrene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic PollutantsBis(2-chloroethyl)ether 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants Butyl benzyl phthalate 4.9 U ug/L 4.9 194/5/2010 12:10 Blank Priority Organic Pollutants Chrysene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants Hexachlorobenzene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants Naphthalene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 7.84/5/2010 12:10 Blank Priority Organic Pollutants Phenol 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Chloro-3-methylphenol 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 7.84/5/2010 12:10 Blank Priority Organic Pollutants Di-n-butyl phthalate 4.9 U ug/L 4.9 194/5/2010 12:10 Blank Priority Organic Pollutants Dibenzo(a,h)anthracene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Diethyl phthalate 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 7.84/5/2010 12:10 Blank Priority Organic Pollutants Pentachlorophenol 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Acenaphthene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Anthracene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Benzidine 97 U ug/L 97 3904/5/2010 12:10 Blank Priority Organic Pollutants Di-n-octyl phthalate 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Dimethyl phthalate 9.7 U ug/L 9.7 394/5/2010 12:10 Blank Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 7.84/5/2010 12:10 Blank Priority Organic Pollutants Phenanthrene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Pyrene 0.97 U ug/L 0.97 3.926Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remark Units MDL PQL4/5/2010 12:10 Blank Priority Organic Pollutants Acenaphthylene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Benzo(b)fluoranthene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Benzo(k)fluoranthene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Isophorone 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2,4,6-Trichlorophenol 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2-Chloronaphthalene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2-Chlorophenol 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2-Nitrophenol 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 3,3'-Dichlorobenzidine 39 U ug/L 39 1604/5/2010 12:10 Blank Priority Organic PollutantsBis(2-ethylhexyl)phthalate 15 UJ ug/L 15 584/5/2010 12:10 Blank Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 2.9 124/5/2010 12:10 Blank Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 4-Nitrophenol 15 U ug/L 15 584/5/2010 12:10 Blank Priority Organic Pollutants Benzo(g,h,i)perylene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Fluoranthene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Fluorene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dichlorophenol 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2,4-Dinitrotoluene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants 2,6-Dinitrotoluene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic Pollutants Benzo(a)anthracene 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Priority Organic PollutantsBis(2-chloroethoxy)methane 0.97 U ug/L 0.97 3.94/5/2010 12:10 Blank Field Parameter Sample Depth 0.1 m

### 27 Appendix F. Facility Summary, Efflue nt Limits, and Permit Violations Recorded on Discharge Monitoring Report

### 28 Appendix F. (continued)

### 29 Appendix F. (continued)

### 30 Appendix F. (continued)

### 31 Appendix F. (continued)

### 32 Appendix F. (continued)

### 33 Appendix G. EPA End Sheet

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 2 0 8 11 12 1 0 0 4 0 5 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 2 0 8 11 12 1 0 0 4 0 5 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 2 0 8 11 12 1 0 0 4 0 5 18 S 19 S 20 2

66 Remarks Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks 

## The Bioassay of FPL- St. Lucie Plant effluent sampled on April 5,
2010. NPDES #FL0002208.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks



## The Priority Pollutants Analysis of FPL-St. Lucie Plant effluent sampled on April 5, 20 10. NPDES #FL0002208.

Biological Analyses of FPL-St. Lucie Plant effluent sampled on April 5, 2010. NPDES #FL0002208.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)