# Harbor Generating Station



# Appendix A Field Sampling and Sample Processing Procedures

A1. Entrainment Field Sampling A2. Entrainment Sample Sorting A3. Entrainment Sample Identification A4. Impingement Field Sampling

### Harbor Generating Station

IM&E Final Report Appendix A1: Entrainment Field Sampling Procedure

A1 - 2 APPENDIX A1: PROCEDURE FOR COLLE CTING PLANKTON SAMPLES FOR ENTRAINMENT STUDIES 1.0 PURPOSE The purpose of this document is to define the steps and equipment necessary to accurately collect plankton samples using a wheeled bongo frame near the Harbor Generating Station (HGS). 

### 2.0 RESPONS IBILITIES 2.1 Task/Field leader:

 Notify the station of the proposed sampling dates.

 Schedule and coordinate sampling surveys and notifying the U.S. Coast Guard prior to sampling.

 Verify that all investigating biologists conducting the sampling have read and understand these procedures.

 Verify that procedures have been followed during sample collection and that the sampling has been conducted safely.

 Verify that information on data sheets have been reviewed and properly recorded.
2.2 Investigating biolog ist:

 Conduct sampling using the following procedures. 

### 3.0 HGS CONTACT INFORMATION

Name Cell/Outside Line E - mail Information Mostafa Haji (310) 522

- 1316 Mostafa.Haji@ladwp.com James Graden (310) 522

- 1752 James.Graden@ladwp.com On

- Site Emergency Number 20300


### 4.0 PROCEDURES 4.1 Mobilization
a. Notify plant personnel of the dates of field sampling prior to the sampling day. b. Ensure there are enough jars, labels, and preservative (formalin) for the sample collection. Print the required number of blank field data sheets on waterproof paper.
c. Inspect the wheeled bongo frame, nets and codends for any damage. If damaged, repairs must be made before sampling begins. Ensure that the flowmeters have been calibrated within the past 90 days and that they are operational. Attach a flowmeter in approximately the center of each frame mouth. d. Ensure that all additional equipment (Table A1

- 1) is in good operating condition. Make repairs if necessary. 4.2 Sample Collection
a. Samples will be collected every six hours in a 24

- hr period (f our cycles) according to the schedule developed by the Task Leader. A survey team consists of at least a boat driver and two investigating biologists to conduct the sampling.

### Harbor Generating Station

IM&E Final Report Appendix A1: Entrainment Field Sampling Procedure

A1 - 3 b. Locate the station using the latitude/longitude coordinates. Determine the water depth with the fathometer and record the water depth on the field data sheet.
c. Ensure that the winch line and a weight (15

- 20 lb salmon ball) are securely attached to the center of the bongo frame. Ensure that the nets, codends and flowmeters are securely a ttached. The nets should be 333

- μ m me s h

. d. Re c or d e a c h f

l o wm e t er ’ s s e ri al numb e r o n t h e f i e l d d at a s h e et ( Att a c hm ent A 1

- 1). Record the number from the flowmeter counter spins on the field data sheet prior to lowering the frame into the water. Record the start time (local time) on the field data sheet.
e. Using the measured marks on the winch cable, lower the frame and nets through the water column until the wheels on the sides of the frame are on the bottom. When the cable starts to slack, the boat is motored fo rward and the cable is retrieved trying to maintain a 45

- degree tow angle. When the frame reaches the surface, carefully pull it into the boat. Verify that the nets have not picked up any sediment from the bottom. If there is any sediment in the nets or co dends, discard both samples by detaching the codends and rinsing the nets of collected material and then reattach the codends. Repeat the sample collection at that station. f. Check that the number of spins on each flowmeter counter to verify that the target volume of 15

- 20 m3 has been collected (number of spins should be about 2,000). If the target volume has not been met with one tow, subsequent tows will be performed at the station until the target volume has been collected. g. If the correct volume has been collected record the end number of spins from each flowmeter on the field data sheet. Subtract the initial number of spins from the end number and record the total on the field data sheet. If the integrity of either or both flowmeter readings is questionab le (e.g., seaweed wrapped around the propellers), discard both samples by detaching the codends and rinsing the nets of collected material and then reattach the codends. Repeat the sample collection at that station. h. Record the end time (local time) on the field data sheet. i. Beginning at the top of the net, rinse the collected material down into the codend. Since the wash water is not filtered and may contain plankton, rinse the net from the outside ensuring that unfiltered water does not contaminate the samp le. Inspect the net to ensure that it has been thoroughly rinsed. Samples will then be carefully transferred to prelabeled jars with preprinted internal labels. The sample from each net will be placed in separate labeled jars. j. Detach the codend from net #1 and rinse the sample from the codend into a labeled sample jar using a squirt bottle containing seawater. Then, using a graduated cylinder or other measuring device, add enough formalin to make a 10%

- formalin seawater solution. Rinse and inspect the coden d of net #1 before reattaching to the net. Follow the same procedure for net #2. Sample preservation should be completed soon after collection. k. I f th e c o l l e cte d m ateri al w i l l f i l l o v er 1⁄2 o f th e s amp l e j a r , s p lit the s amp l e i nto at l e a s t tw o l ab el e d jars so that there is enough ethanol for proper preservation. l. Ensure that the sample jar contains both an inner label and an exterior label.
m. The following is an explanation of the coding for the field datasheet survey and station numbers and jar labels:
n. Each survey number on the data sheet consists of a series of 5 letters followed by 2 numbers ( H G S EA # # )

. Th e f

i rs t thre e l e tt e rs ar e “ H G S ” re f e r s t o H arb o r G e n e rati n g S t ati o n , and th e “ EA ” refers to entrainment abundance. The two numbers refer to the survey number with the first survey being 01. The survey number increases by one for each new 24

- hour sampling effort.
o. The station designation consists of a letter

- number

- letter

- number combination. The first letter refers to the station being a Harbor or Entrainment station (see map in Attachment A1

- 1). The first number refers to the number of the station that links to the station letter. The second letter designates the rep l i c at e , e i th e r “ A ” o r “ B ”

. T h e s ourc e w at er s t ati o n s o nly h av e o n e s amp l e s o wi l l alw ays u s e th e

### Harbor Generating Station

IM&E Final Report Appendix A1: Entrainment Field Sampling Procedure

A1 - 4 l ett e r “ A ”

. Th e r e are tw o s a mp l e s c o l l e ct e d at t

h e e ntrai n

m e nt l o c ati o n s o th e l ett e rs “A ” an d “ B ” will be used to separate these two replicates. The second number designates the net number, either
“ 1 ” o r “ 2

. ” F o r e x amp l e , H 3 A 1 m e an s th at th e s amp l e w a s c o llected from Station H3, Sample A, and Net 1. p. The date of sampling will correspond to the actual start date of each sample. At the start of a new day (midnight), use a new field data sheet. q. Deliver the samples to the laboratory at the completion of the samp ling effort.

4.3 Sample Voiding in the Field
a. Samples should be voided if any of the following occurs: 1) possible flowmeter obstruction due to kelp or other debris on the propeller, 2) obviously malfunctioning or damaged flowmeters; 3) damaged (torn) nets found after a sample is collected; 4) large quantities of sediment in the net that were collected when the wheeled bongo frame was on the bottom; 5) gear failure which prevents completion of any tows/hauls; 6) an incident or situation which may prevent relia ble data collection; 7) an incident or situation which may jeopardize the safety of sampling personnel. b. If a hole or tear is found in the net mesh, mark the damaged area and either repair or replace the net. Discard both samples and repeat the sample coll ection. Record this on the data sheet.
c. The number of flowmeter spins from the paired bongo nets needs to be checked in the field to confirm that the measured volumes were similar.

### Harbor Generating Station

IM&E Final Report Appendix A1: Entrainment Field Sampling Procedure

A1 - 5 Table A1 - 1 . Equipment List. 1. Wheeled bongo net frame, attached 333/335 mi cron mesh nets, codends, and calibrated flowmeters (include at least 1 back up net and flowmeter) 2. Winch (davits) and line for net deployment and retrieval 3. Stock solution of formalin 4. Squeeze bottles 5. Labeled jars for sample storage 6. Data s heets, pencils, permanent markers, and labels 7. Wash

- down pump 8. Watch 9. Fathometer 10. GPS

### Harbor Generating Station

IM&E Final Report Appendix A1: Entrainment Field Sampling Procedure

A1 - 6 Attachment A1 - 1 . Example field datasheet for HGS station sampling. Harbor Generating Station (HGS) Entrainment Abundance Field Data Sheet Sheet #: Date: Mesh: Flowmeter 1: Conversion 1: Survey #: H G S E A Crew: Net Dia.: Flowmeter 2: Conversion 2:

Station Flowmeter

Start Flowmeter

End
1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 Survey: HGSEA## ( H arbor G enerating S tation E ntrainment A bundance) NOTES: Date: MM/DD/YY Station: A#A# A#: (Station Designation - H arbor 1-6, or E ntrainment 1) A (Replicate A or B - Only Entrainment samples have two replicates) #: (Net 1 or 2) Tide: E bb, F lood, H igh S lack (HS), or Low S lack (LS)
Reviewed By / Date: Entered By / Date: Copied By / Date: (ft) (PST) Station Depth (min)

Start Time (PST) Total

End Time (E, F, HS, LS) Temp Cycle Tide (A#A#) (ppt) 0.335 mm 0.60 m
Sample Number Salinity Total Flow (cu. m)

Volume o C (1-4)

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IM&E Final Report Appendix A1: Entrainment Field Sampling Procedure

A1 - 7 Attachment A1 - 2 . Map of HGS entrainment (E1) and source water (H1

 6) sampling stations.

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 1 APPENDIX A2: PROCEDURE FOR SORTING PLANKTON SAMPLES IN THE LABORATORY
1.0 PURPOSE The purpose of this procedure is to define the steps for sorting target organisms from plankton samples collected at Harbor Generating Station, and to descri be the Quality Control Program (QC) used to monitor the sorting accuracy of individual sorters.


### 2.0 RESPONSIBILITIES

 Laboratory Supervisor is responsible for assuring that plankton sample sorting is in accordance with written procedures.

 The Quality Contr ol Supervisor is responsible for implementing the Quality Control Program, which monitors sorting accuracy in accordance with written procedures.

 Investigating biologists are responsible for sorting samples in accordance with written procedures.


### 3.0 INSTRUCTIONS 3.1 Sorting Procedures 3.1.1 Sample Processing
a. Ensure that the proper equipment necessary for sample processing is available (Table A2

- 1). b. Samples that were originally fixed in formaldehyde after collection must be transferred to 70% ethanol before laboratory processing. This is done outside to lessen the exposure to formaldehyde fumes. Only qualified personnel who have read and signed the information about the hazards of working with formaldehyde may transfer samples. 1. A funnel with the appropriate mesh size attached to its bottom opening is placed into a jar or can. The mesh must not be larger than that used during sample collection. Place the jar and funnel in a tray so the sample can be retrieved if spillage occurs. 2. Pour the sample carefully into the canning funnel. The sample jar and jar lid are rinsed with water, directing the water and organisms into the funnel. Rinse the sample with water to flush the formaldehyde from the sample. 3. Rinse the sample into a labeled jar with 70% ethanol from a sq ueeze bottle. Make certain that the jar has both an inner label and a jar top label. Additional ethanol is added to the sample jar to cover the sample. 4. The waste formaldehyde and rinse water is then discarded into the appropriate hazardous waste container.
c. Consult the sorting schedule posted in the processing laboratory to determine sorting priorities. d. Sign out the sample in the Laboratory Tracking MS Excel file and the Laboratory Sample Tracking Sheet (Attachment A2

- 1 ) b y wri t i n g yo ur i nit i al s un d e r t h e ‘ s o r t e r ’ c o lumn . Tr an s crib e i n f

o rm ati o n f

ro m t h e s am p l e l a b e l i nt o t h e S o r t e r ’ s L o g B o o k ( Att a chm e nt A2

- 2) and into the sorter's notebook (each sorter has separate log sheets and a notebook for this purpose).

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 2 e. Take two clean canning funnels with attached me s h n ettin g , o n e l ab e l e d ‘ s o r t e d ’ a n d th e o t h e r l ab e l e d ‘ un s o rt e d ’

. Th e m e s h s i z e s h o ul d b e n o l arg e r th a n th at u s e d t o c o l l e ct th e samples. f. P l a c e th e ‘ un s o r

t e d ’ c anni n g f

unn e l o v e r th e ‘ u s e d al c o h o l ’ b ottl e a n d f unn e l , whi c h i s located in a dish so samples can be retrieved if a spill occurs. Pour the sample into the canning funnel. The canning funnel will contain the material to be sorted, while the e th a n o l wi l l drai n i nto th e ‘ u s e d al c o h o l ’ b o tt l e . g. Using 70% ethanol or 70% used alcohol in a squeeze bottle , rinse any remaining sample from the sample jar, the jar lid and inner sample label into the canning funnel containing the unsorted sample. h. P l a c e th e ‘ un s o r

t e d ’ f unn e l c o nt ai nin g th e s amp l e a n d th e emp ty ‘ s o rt e d ’ f

unn e l i nt o individual glass bowls in a tray. Make sure the sample is covered with water so it will not dehydrate during processing. i. U s i n g f

o rc ep s , tra

n s f er a s m al l am o unt o f th e s amp l e f r o m th e ‘ un s o r

t e d ’ f unn e l t o t h e sorting tray. Add enough water to cover the sample. Distribute the sample in the sorting tray. j. Place the sorting tray on the base of the dissecting microscope. Adjust the magnification so that the field of view is slightly larger than the width of an individual marked grid. k. Arrange the light source to provide adequate illuminatio n. l. Carefully scan the entire sorting tray using the grids for orientation. Remove the target organism with forceps and place them either into a shell vial containing 70% ethanol or into a small dish containing water. Count the organisms as they are remove d. A list of what target organisms and when to pull them is posted in the lab.
m. Log the number of organisms removed from the sample in the sorter notebook.
n. Scan the tray a second time. If target organisms are found on the second pass, repeat a third time. C ontinue this process until a scan does not produce any additional target organisms.
o. O n c e s o r

t e d , p o ur th e s o r

t e d s amp l e i nt o th e ‘ s o rt e d ’ f unn e l an d ri n s e with a s m al l am ount o f w at er . T ake a s e c o n d al i qu o t f ro m th e ‘ un s o rt e d ’ f

unn e l a s d e s c rib e d ab o ve . Repeat the above steps until the entire sample has been sorted. p. If the sorter thinks there will be more than 500 fish eggs in a sample then the sample may b e “ s u b s a mp l e d ” f o r e g g s . Wh e n “ sub s amp l i n g ” th e s amp l e s h oul d b e p ro c e s s e d f i rs t f o r fish larvae an d s e l e ct e d i nv e rteb rat e l a rv a e . Wh e n re a dy t o “ s ub s amp l e ” put th e s o r t e d sample back in the original sample jar and fill the jar with 70% ethanol up to the lip of the jar. Jar size varies, but they will typically be 500 ml (if sizes varies there will be a posting i n th e l ab )

. A ‘ sub s amp l e ’ s houl d b e 1 0 p e r c e nt o f th e s am p l e v o lum e s o th e s o r

t er wi l l use the aliquot transfer pipette with the 10 ml attachment and take 5 aliquots. The sample should be stirred up in order to get a fair amount of sample in the aliquot. Once the aliquot is processed for fish eggs it may be returned to the original sample jar with the rest of the sorted sample. Make sure it is noted in the logbook and record the total volume of the sample and the volume of the sub sample. There will be an extra data sheet in the laboratory tracking sheets and a column in the MS Excel tracking sheets to record the sub sample information. On top of the sample jar put a white dot with survey number, sample number, sorters initials, sub sample date, and “ S S ”

. q. When the sorting has been completed, the sorted organisms should be placed into a shell vial containing 70% ethanol. Fill the shell vial completely with clean 70% ethanol then place cotton into the top end of the vial to keep the organisms insid e. Place the vial into a labeled snap cap containing 70% ethanol. Make sure the shell vial and cotton are completely covered with 70% ethanol.

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 3 r. Label each jar lid with the appropriate colored dot label. Prepare a waterproof inner label for the jar contai ning the shell vial. Both labels should contain the following information: 1. Survey number 2. Collection date 3. Station, cycle and sample number 4. Collection start time 5. Jar number (if more than one jar) 6. S o rt e r ’ s i ni ti al s 7. Number of organisms in shell vial
s. The total number of sorted organisms and the total time required to process the sample is recorded in the sorter's notebook. t. Put the sorted sample back into the original sample jar. Used alcohol may be used to fill s amp l e j ar to at l e a s t 3⁄4 f

ull

. Ri n s e an y re m ai n in g sample from the funnel into the jar using a squirt bottle containing ethanol. Make sure the inner waterproof label is in the sample jar. Thoroughly clean the funnels of all remaining sample.
u. If a sample must be stored before completion: 1. Put the sorted po rtion of the sample back into the original sample jar. Rinse any remaining material from the funnel into the jar using a squirt bottle containing ethanol. Make sure that the sample is adequately covered with ethanol. 2. Put the unsorted sample into a second j ar . Ri n s e any s amp l e f ro m th e ‘ un s o rt e d ’ funnel into the jar using a squirt bottle containing ethanol. Using a dot label, label the j ar l i d wi th th e s a

mp l e i d e nti f

i c ati o n in f

orm ati o n , s o rt er ’ s ini ti al s , a n d th e w o rd
“un s o r t e d ”

. M ake an ad diti o nal i nn e r l ab el with the sample identification information an d m arke d ‘ un s o r

t e d ’

. P l a c e th e l a

b e l i n s i d e th e j ar wi th th e ‘ un s o rt e d ’ s a mp l e . En s ur e th at th e ‘ un s o r

t e d ’ s amp l e i s a d e qu at e l y c o v e re d wi th e th a n o l

. 3. The sorted and unsorted portion of the sample should be st ored until sorting can continue. 3.1.2 Once the sample is completed, place an appropriately colored dot label on the jar top with the sorter's initials and date of sorting. Return the jar to the box from which it was originally removed.
a. Transcribe the information recorded in the sorter's notebook to the computer on the Laboratory Tracking Sheets and the Quality Control log and on the Laboratory Sample Tracking Sheet (Attachment A2

- 1), and to the Sorter's Log (Attachment A2

- 2). 3.2 Sorting Quality Contro l Program 3.2.1 QC Sorting Criteria
a. The first ten samples that are sorted by an individual are completely resorted by a designated QC sorter. A sorter is allowed to miss one target organism when the original sorted count is 1

 19. For original counts ab ove 20 a sorter must maintain a sorting accuracy of 90%. b. A f t e r t h e s o rt e r h a s p a s s e d 1 0 c o n s e cuti v e s o r t s , th e pro gram i s s wit c h e d t o a ‘ 1 s a mp l e i n 1 0 ’ Q C pr o gram f

o r th at s o rt er . A f

t er th e s o r

t er h a s c o m

p l et e d an o th er 1 0 s am

p l e s , o n e sample is randoml y selected by the designated QC sorter for a QC resort.
c. If the sorter maintains the 90% accuracy sorting rate for this sample, then the sorter c o ntinu e s i n th e ‘ 1 s amp l e i n 1 0 ’ Q C m o de . d. If a sample does not meet the 90% accuracy rate their subseque nt samples will be resorted until 10 consecutive samples meet the criteria.

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 4 3.2.2 QC Resorting
a. Sorting procedures used during the QC resort are the same as the sorting procedures described in Section 3.1. b. All fish and selected invertebrate larvae that were missed by the sorter are removed during the QC resort.
c. For the QC process, a larval fish is defined as having a head plus at least 50% of the body. Any parts without a head and/or less than 50% of the body will be considered fragments and will not be counted against the original sorter as a missed fish. However, it is important for each sorter to remove all fish and fragments from each sample that is sorted an d c o r re ct ly re c o rd th em a s # f

i s h / # f ragm e nt s i n th e s o rt er ’ s n o t eb o o k an d o n th e tr acking sheet. d. Any vials of fish larvae or selected invertebrate larvae generated from the resort are labeled with an orange dot label, and labeled as described in the sorting procedures with th e a ddi ti o n o f “ Q C ” a d de d t o th e l a b e l

. e. An orange dot lab el should also be placed on the top of the jar of the sample that was re s o r t e d an d l a b e l e d with th e Q C p e r s o n ’ s i niti al s , surv e y numb e r , s amp l e num b e r , and date the resort was completed. f. The vials are stored in the appropriate location. 3.3 Waste Dispo sal 3.3.1 No formaldehyde or water contaminated with formaldehyde should be disposed of into the sewage system. Dispose of any water contaminated with this chemical in the designated waste water container to be disposed of at a local hazardous materials waste d epository. 

### 4.0 RECORDS 4.1 All data sheets are later reviewed by the Lab Manager or designated staff. 4.2 Original data sheets are permanently stored.

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 5 Table A2 - 1. Equipment List
1. Tray or dish 2. Bowls 3. Sample jars 4. Two canning funnels with attache d plankton mesh

n e tti ng , l ab e l e d with m e s h s i z e , an d l ab e l e d ‘ s o rt e d ’ and ‘ un s o r

t e d ’ 5. Squeeze bottle containing 70 percent ethanol

(denatured) 6. Squeeze bottle containing fresh water 7. Sorting tray or petri dish marked with a sorting grid 8. Disse cting microscope with light source 9. Glass shell vials and cotton 10. Jar/vials with lids 11. Forceps 12. Waterproof labels 13. Dot labels 14. Sorter's notebook 15. Plankton splitter

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 6 HARBOR GENERATING STATION (HGS)
– Lab Tracking Sheet 

### Survey 

### : Sample Information Sort Information QC Information

Project # Sample

Date Station Cycle Sample Start Time # Jars Sorter Date Sorted Time

(hrs) # Fish Fish Eggs # Megs # Lobster/ Squid Q C ’ d B y QC Date QC Fish QC Other *

*specify which (fish eggs(E), megs(M), lobst er(L), or squid(S)) 

### Attachment A2 - 1. Lab Tracking Sheet

### Harbor Generating Station

IM&E Final Report Appendix A2: Entrainment Sample Sorting Procedure

A2 - 7 

## Harbor Generating Station - Sorter's Log

(HGSEA) Name: Sample Information Sort Information Quality Control Sort Ti me
Survey Number Station Cycle Sample Collection Time Date

Sorted # of

Fish # of Fish Eggs # of Megs Lobster/ Squid QC'ed

By QC'ed Date QC Fish Fish P / F QC Other
* Other P / F

*specify which (fish eggs(E), megs(M), lobster(L), or squid(S))


### Attachment A2 - 2 S o r

t e r ’ s L o g S h e et

### Harbor Generating Station

IM&E Final Report Appendix A3: Entrainment Sample Ident ification Procedure

A3 - 1 APPENDIX A3: PROCEDURES FOR THE IDENTIFICATION OF LARVAL FISHES and TARGET INVERTEBRATES
1.0 PURPOSE The purpose of these procedures is to define the steps for identifying planktonic organisms, and to describe the Qu al ity C o ntr o l ( Q C ) P ro gram u s e d t o mo ni t o r t h e a c c ura cy o f e a c h i n di vi dual ’ s i d e nti f i c ation performance. 

### 2.0 RESPONSIBILITIES

 The Lead Taxonomist is responsible for assuring that plankton identifications are performed in accordance with written procedures and for implementing the Quality Control Program.

 Investigating biologists are responsible for plankton identifications and for monitoring accuracy in accordance with written procedures. 

### 3.0 INSTRUCTIONS 3.1 Identification procedures for larval fishes, Cancer spp. crab and Panulirus lobsters.

a. Ensure that the proper equipment necessary for the identi fication of target organisms is available (Table A3

- 1). b. The fish and target invertebrates from each sample are kept in separate containers and processed following this procedure in essentially the same manner.
c. The container of target organisms to be identi fied is carefully emptied into a dish. The dish is placed on the microscope stage and the lighting adjusted to provide adequate illumination. d. Each target organism is identified to the lowest taxonomic classification possible. The total number of each taxon is recorded on the Entrainment /Source Water Plankton Tow Lab Data Sheet (Attachment A3

- 1).
e. All individuals of each identified taxon of larvae from a sample should be put into a shell vial containing 100% ethanol. Each vial should contain a label with th e taxon name and sample number. Cotton should be pushed into the upper end of the vial to keep the label and organisms enclosed. f. Mutilated larvae (partial organisms that are missing body parts and are unable to be identified) are placed in a separate labe led vial. Whole larvae that are unidentified, are placed in a separate labeled vial. g. All vials containing target organisms from an individual sample should be put into a labeled jar containing enough ethanol to cover the vials. The jar should contain both an inside label and a label attached to the outside of the lid denoting the sample number, date and time c o l l e ct e d , an d i de nti f

i e r ’ s i niti al s . T i ght en th e j ar l i d t o p re ve nt e v ap o r ati o n o f th e preservative. Samples with many different fish taxa may require more than one labeled jar. h. O n th e L ab o rat o ry S amp l e Tr ac ki n g S h e e t , re c o r d t h e i d e nti f i e r ’ s i niti al s a

n d d at e s am

p l e w a s l o g g e d i n . Th e i d e nti f

i e r ’ s l o g wi l l c o nt ai n t

h e t o t al numb e r o f l arv a e i d e n ti f i e d a n d th e d ate identified. If more than one day was n eeded to complete the identification, record the date the sample identification was completed. i. Place the jar into the appropriate box containing identified samples. j. Dispose of any liquids containing ethanol into the appropriate waste container.

3.2 Identi fication Quality Control (QC) Program

### Harbor Generating Station

IM&E Final Report Appendix A3: Entrainment Sample Identification Procedure

A3 - 2 3.1.2 Fishes
a. The first ten samples of larval fishes that are identified by an individual identifying biologist will be completely re

- identified by a designated identification QC biologist. A total of at least 50 individuals from at least 5 taxa (50/5 criteria) must be present in these first ten samples. If the first 10 consecutive samples do not pass the 50/5 criteria, additional samples must be re

identified until this criteria is met. b. The identifying biologist must maintai n a 95% identification accuracy level in these first 10 samples. For all samples, if a sample contains between 1

 19 larvae, one larvae can be misidentified and the sample will not fail the QC check.
c. If the identifying biologist identifies a larval fish to a certain family or genus and subsequently the identification QC biologist is able to refine the identification to a lower taxonomic level, this will not be considered a misidentification pertaining to the 95% identification accuracy level. A misidentification will be one in which the identifying biologist identifies the fish as belonging to a certain family, genus or species, and then the identification QC biologist determines that the initial identification was incorrect and changes the identification to a different family, genus or species or changes it to a higher taxonomic group. d. After the identifying biologist has passed 10 consecutive samples, the program is switched to a “ 1 s am p l e i n 1 0 ” Q C p ro gra

m . A f t er th e i d ent i f

yi ng b i o l o g i s t h a s c o m

p l e t e d ano ther 10 samples, one sample is randomly selected by the designated identification QC biologist for a QC review.
e. If this sample maintains the 95% accuracy level as determined by the identification QC biologist, then the identifying biologist continues in th e “ 1 s amp l e in 1 0 ” Q C m o d e . I f a sample does not meet the 95% accuracy level, their subsequent samples will be re

- identified until 10 consecutive samples meet this level of accuracy. f. Any misidentified fish found by the identification QC biologist, will be placed into the appropriate labeled vial for that sample. This information will be recorded on the Fish Identification Data Sheet. 3.1.3 Cancer spp. and Panulirus spp. a. The first ten samples identified by an individual identifying biologist will be completely re

identified by a designated identification QC biologist. b. The identifying biologist must maintain a 95% accuracy level in these first 10 samples. For all samples, if a sample contains between 1

- 19 larvae, one larvae can be misidentified and the sample will not fail the QC check.
c. After the identifying biologist has passed 10 consecutive samples, the program is switched to a “ 1 s am p l e i n 1 0 ” Q C p ro gra

m . A f t er th e i d ent i f

yi ng b i o l o g i s t h a s c o m

p l e t e d ano t

h e r 1 0 samples, one sample is randomly selected by the de signated identification QC biologist for a QC review. d. If this sample maintains the 95% accuracy level as determined by the identification QC b i o l o g i s t , th e n th e i d e nti f yi n g b i o l o gi s t c o nti nu e s i n th e “ 1 s amp l e in 1 0 ” Q C m o d e . e. I f an i d enti f

i er ’ s s am p l e d o es not meet the 95% accuracy level, their subsequent samples will be re

- identified until 10 consecutive samples meet this level. f. Any misidentified larva found by the identification QC biologist, will be placed into the appropriate labeled vial for that sample and recorded on the appropriate laboratory identification data sheet.

### Harbor Generating Station

IM&E Final Report Appendix A3: Entrainment Sample Identification Procedure

A3 - 3 3.2 Larval Fish Measuring 3.2.1. Larval Fish Measuring Procedure g. Turn on the computer, camera, and light source at the measuring station. h. Consult the measuring schedule near the measuring station to determine measuring priorities and retrieve the binder containing the appropriate data sheets. i. Locate the box containing the fish to be measured and place it in a easily accessible area close to the measuring station. j. Open the Optimas Image Analysis or ImageJ software by clicking with the mouse on the appropriate software icon. k. Open the Larval Fish Measuring macro in Optimas, or the FishMeasure2 macro in ImageJ and f o l l o w t h e m a c ro s ’ di re cti o n s . l. Select the jar of fish to be measured and consult the jar label. Compare data on the jar label with the inner label and the data sheet for this sample. Consult an identifier regarding discrepancies between labels.
m. Enter the data queried for by the macro including the last five digits of the seria l number, the m e a s ur er ’ s ini ti al s , th e d at a s h e e t s e qu en c e numb e r a n d th e sp e c i e s c o d e . n. Open the jar and remove the vials for the target taxa to be measured as per the posted list. Place the vials in a rack designed to allow the vials to maintain an uprigh t posture so as to reduce spillage.
o. Select the first vial to be measured. Remove the cotton and the label. Compare the label with the data sheet for confirmation. p. Empty the vial into a shallow dish. Remove any fish that have adhered to the vial, cotton, th e label, or any tools used in the transferring process and place the fish in the dish. Add alcohol to the dish if necessary to prevent desiccation. q. If the number of larval fish in the vial exceeds what can be reasonably measured on a single image capture, transfer some of the fish to another glass dish and immerse them in alcohol.
r. Place the dish on the stage of the microscope. Arrange the fish so that all fish appear on the screen. Adjust the zoom, focus, and lighting for the best possible image. If this is the first group of larval fish being measured, or if the magnification has been changed, it is necessary to re

- calibrate. Place the micrometer on the stage of the microscope and re

- calibrate by drawing a line from one of the micrometers millimeter marks t o another, noting the distance between the two marks, and entering that value when queried. Replace the dish containing the larval fish to be measured.
s. Measure larval fish by drawing a line from the pre

- maxillary to the end of the notochord, being careful to follow the contours of the fish. If the fish is too damaged to find either the pre - maxillary or to estimate the path taken by the notochord, do not measure, and proceed to the next larval fish. If the line does not adequately approximate the larval fish ’ s l e n gth i t m u s t be re

- measured. t. N o t e th e p ro gram ’ s di s p l ay o f th e m e a s ure m e nt , ch e c k th at it s e e m s re a s o n ab l e . I f it d o e s no t seem reasonable, it may be necessary to re

- calibrate and re

- measure. If the problem persists, contact an identifier. Make note o f any problems in measuring and post near the measuring station.
u. The macro will store the measurement in separate data files along with the necessary sample information.
v. Repeat the above steps for all fish in the dish.

### Harbor Generating Station

IM&E Final Report Appendix A3: Entrainment Sample Identification Procedure

A3 - 4 w. When all larval fish in the dish hav e been measured, fill the vial that originally contained the fish with alcohol and transfer the measured fish to the vial.
x. If the larval fish from this vial have been segregated into two or more groups, place another group into the dish, being careful to s ubmerse them in alcohol, and measure as above. Do not measure more than fifty larval fish of any one taxon from each survey. 

### 4.0 RECORDS 4.1 All data sheets are later reviewed by the Lab Manager or designated staff. 4.2 Original data sheets are permanently sto red.

### Harbor Generating Station

IM&E Final Report Appendix A3: Entrainment Sample Identification Procedure

A3 - 5 Table A3 - 1 . Equipment List 1. Dissecting microscope, with camera attachment connected to computer equipped with Optimas 6.2 or ImageJ if measuring larvae 2. Light source 3. Micrometer 4. Sorting tray or petri dish 5. Squeeze bottle containing 70% ethanol (denatur ed) 6. Glass shell vials 7. Holder for shell vials 8. Jar containing target organisms to be identified 9. Cotton 10. Forceps 11. Waterproof labels 12. Dot labels 13. Data sheets 14. I d e nti f

i e r ’ s l o g s h e e t 15. Taxonomic references

### Harbor Generating Station

IM&E Final Report Appendix A3: Entrainment Sample Identification Procedure

A3 - 6 Attachment A3 - 1 . Entrainment /Source Water Plankton Tow Lab Data Sheet HARBOR GENERATING STATION (HGS) - Lab Data Sheet Sheet:
Start Time: Cycle: Station:

Taxon

NOTES: Total: Count Count Survey: Date:
Species Code Comments

Entered By / Date Stage Entrainable

Fish Eggs Inverts QC Resort Additional Total

ID'ed By / Date ID QC By / Date QC Resort By / Date Count

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 1 APPENDIX A4: IMPINGE MENT FIELD SAMPLING PROCEDURES 1.0 PURPOSE The purpose of this document is to identify the procedures and equipment necessary to accurately collect and process impingement samples at the Harbor Generating Station (H GS).


### 2.0 RESPONSIBILITIES 2.1 Task/Field leader:

 Contact plant to obtain clearance for personnel that will be conducting the sampling.

 Verify that all investigating biologists conducting the sampling have read and understand these procedures.

 Verify that procedures have been followed during sample collection and that the sampling has been conducted safely.
2.2 Investigating biologist:

 Conduct sampling using the following procedures. 

### 3.0 HGS CONTACT INFORMATION
Name Cell/Outside Line E - mail Information Mo stafa Haji (310) 522

- 1316 Mostafa.Haji@ladwp.com James Graden (310) 522

- 1752 James.Graden@ladwp.com On

- Site Emergency Number 20300


### 4.0 PROCEDURES Impingement sampling will only be undertaken when there is water passing through the traveling screens fo r Unit 5. If only one pump is in operation, proceed with sample collection using the following procedures. Each normal operation impingement survey consists of an initial cleaning period followed by four 6

- hr sampling cycles. Table A4

- 1 presents the propos ed schedule for each survey. 4.1 Mobilization
a. Notify plant personnel of the dates, times, and names of the biologists that will be onsite during each s urv e y . A l l p e rs o nn e l wi l l r e q ui re a p h o t o i d e nti f

i c ati o n ( dri v e r ’ s l i c e n s e , p a s sp o r t , et c . ) t o ob t ain access to the plant site. b. The equipment listed in Table A4

- 2 is required for sampling and should be checked before leaving for the plant. Verify that any scales used for the sampling have been calibrated within the previous three months.

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 2 4.2 Traveling Screen Sample Collection Material impinged at Unit 5 is rinsed from the traveling screens into a metal collection basket placed in the rectangular sump. Figure A4

- 1 shows the traveling screens (TS) area of Unit 5 and Figure A4

- 2 shows the rectangular sump whe re impinged materials accumulate.
a. Make sure that the collection basket has been emptied prior to the survey. If there is any debris in it, contact an operator to have them remove the basket using a crane so that it can be emptied. b. The initial screenwash i s for cleaning purposes only; do not process any materials from the sump after the cleaning rinse. The time at the end of the initial TS rinse is the beginning of Cycle 1. Record this on the appropriate datasheet.
c. The mesh in the collection basket has a larger mesh than the mesh of the TS. Secure a mesh net/vexar basket (with mesh of the same size or smaller than the TS mesh) inside of the metal basket so that impinged materials can easily be removed. If the debris load is too heavy for the mesh net/vexar b asket or it is damaged and materials accumulate in the metal basket, make sure an operator is available to use a crane to pull the metal basket out for sample collection. d. Have plant personnel activate the TS wash system thirty minutes prior to the end of e ach cycle so that all impinged material is rinsed from the screens into the collection basket.
e. Remove all impinged fish and invertebrates from the impinged debris. f. Replace the mesh net/vexar basket in the rectangular sump before initiation of the screenwas h for the next cycle. g. All collected impinged material will be processed using the procedures in following section. 4.3 Sample Processing
a. Remove all fishes and invertebrates from the impinged debris. Record the volume of the debris (gallons) on the datasheet. A lso record the composition and percentage of the debris. b. All fishes, crabs, shrimps and prawns, and cephalopod mollusks are identified, counted, measured (see measurement criteria below), and weighed. This information is recorded on the appropriate datashe et
. Al l o th er i nv e rteb rat e s are i d enti f i e d an d re c o rd e d a s p re s e n t b y e nt e ri n g a “ P ” i n t

h e count box. Organism Group Length Measuring Criteria Fishes Total body length for sharks, disc width for skates and rays and standard lengths for bony fishes Crabs Maximum carapace width Spiny lobster and Shrimps Carapace length, measured from the anterior margin of carapace between the eyes to the posterior margin of the carapace Octopus M axi mum “ arm ” s pre a d , m e a sure d f ro m th e tip o f o n e t ent a c l e t o t h e tip of th e opposite tentacle Squid Dorsal mantle length, measured from the edge of the mantle to the posterior end of the body

c. Record all organism names on the appropriate datasheet, using their scientific names whenever possible. The taxa codes are recorded aft er the datasheets are returned to the office. d. Make certain of all identifications before recording the name on the datasheets. If an organism cannot be positively identified it should be saved. The voucher specimen should be placed in a plastic bag with a waterproof label indicating the location, cycle, date, time, and the initials of the c o l l e ct o r . I f p e rs o nn e l o n th e n e xt c yc l e c an p o s i ti v e ly i d e nti f y th e o r g ani s m re c o rd th e o rg an i s m ’ s name on the datasheet. If positive identification cannot be made the o rganism is returned to the laboratory for identification.

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 3 e. If a large number (more than 30) of any individual countable species is collected during a cycle, individually measure and weigh 30 randomly selected individuals of this species and then count and measure the remaining individuals and record this information on a separate line on the datasheet. For example: if 198 deepbody anchovies were collected, randomly select 30 individuals and record on the first row Anchoa compressa , count = 1, Length = standard length (the distance from the tip of the snout to the posterior vertical margin of the hypural plate) to the nearest mm, weight = weight to n e are s t gram , s e x = “

- “ ( i f th e s e x c ann o t b e d et e rmi n e d wi t ho ut di s s e cti o n , r e c o rd a “

- “ ) , a n d condition = alive (A), dead (D), or mutilated (M). Continue this procedure for the other 29 randomly chosen deepbody anchovies. Then batch weigh the remaining individual. If all of the individuals are al i v e , put an “ A ” i n th e C o nd

. b o x . I f s o m e are d e a d an d s o m e mut i l at e d , use additional rows to fill in the appropriate information in the corresponding row(s). f. Determine the sex of the countable organisms to the extent possible without dissection. Assign the letter M to refer to males, F for females, J for juveniles, G for gr avi d

. P ut a “

- “ i f th e s e x c ann o t b e determined without dissection. g. Record the condition of each countable organisms: A for alive; D for dead; M for mutilated. If an individual is mutilated, do not measure the length. If there are more than 30 non

- mutilated individuals, the mutilated individual(s) can be weighed with the batch weight of the additional individuals. If there are less than 30 non

- mutilated individuals, record the weight of the mutilated individual(s) but not their lengths. h. Record any anomalie s or other notes (encountered in each cycle) in the notes section on the datasheet. i. At the end of each cycle verify that: a) the sampling procedures have been followed correctly, b) the data has been recorded correctly and legibly, and then c) sign and dat e th e “ Re vi e w e d b y /D at e ” section at the bottom of the datasheet. j. Put all dead animals and discarded debris in trash dumpsters. Make sure to double bag the material as collection of the trash may not occur for several days. k. Quality control (QC) checks will be preformed on at least a quarterly basis to verify all organisms are being removed from the debris and that the correct identification, enumeration, length and weight measurements of the organisms are being recorded on the datasheet. The QC team will ran domly choose the actual impingement cycles that will be checked and will resort the debris for any missed organisms. All organisms will then be identified, re

- measured and re

- weighed by the QC team to ensure that the data is being recorded correctly. If a sampling team fails a QC check, they will be retrained on fish identification and sample collection. QC checks will be performed on the sampling team until they pass the QC requirements. The QC checks will be fully documented and reported to the Project Manager. 4.4 General
a. All information recorded on the datasheets must be written legibly with a pencil. b. Keep information separate for each cycle
c. The survey number will be determined based on the week corresponding to that survey (e g., week 1 = survey 1). Make sure the correct survey number is recorded. d. Make certain that the unit #s and cycle numbers are correct on the datasheet you are using. Record the date and time for the start and end of each screen wash (generally 15 to 20 minutes) and cycle duration times (generally 6 hours). Each 24

- hr survey is divided into 4 six

- hour cycles.
e. Record the names of all personnel present during each cycle. f. Use military time (0000

– 2400) to record every cycle collected. Record all times as local time (Pacific Standard Time o r Pacific Daylight Time).

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 4 g. During each screen wash, verify that the screens are operating properly (the screens should be moving and the water should be spraying). Check with the operator to find out how many circulating water pumps are operating. h. If a surv ey cannot be completed or is cancelled, make a note on the appropriate data sheet explaining the reason for the cancellation. Write the survey number that corresponds to that week, date and sign the datasheet. i. At the end of each screen wash, record the rel evant meteorological data. j. If the traveling screens trip before the 6

- hour cycle is over, collect all material and process it as part of the upcoming cycle. If possible, have the screen wash system run at the scheduled times. 4.5 Heat Treatments During heat tr eatments follow the same procedures as during normal impingement sampling. Use a separate data sheet for each species collected. A single data sheet can be used for several species if low numbers of these species are collected. If an extremely large amount of material is collected in the sump basket, sub

- sampling of the most abundant fishes/shellfishes should occur to minimize the time taken to process the sample. Sub

- sampling procedures are as follows:
a. Remove the less abundant fishes/shellfishes from the i mpinged material; and record as individuals on the datasheets. b. Collect a sub

- sample (for example, two 3

- gallon sub

- samples) from the pile of impinged material. Make sure to randomly sample the pile by collecting organisms from different areas of the pile. Discard the remaining material and record the volume discarded on the data sheets.
c. The number and weight of the organisms collected from each sub

- sample should not be recorded with the other data. Record the sub

- sample data on a separate datasheet. Make c ertain that record of the organisms from the sub

- samples can be linked back to the quantity of material discarded and not sampled.

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 5 Table A4 - 1

. Target schedule for 24

- hour impingement sampling effort (schedule assumes that at least one circulating water p ump is in operation). Based on 30 minutes for complete rotation of travel screens at each unit. Time Unit 5 8:00 Rinse and Clean 8:30 Start Cycle 1 9:00 9:30 10:00 10:30 11:00 11:30 12:00 12:30 13:00 13:30 14:00 Rinse Cycle 1 14:30 Start Cycle 2 15:00 Process Cycle 1 15:30 16:00 16:30 17:00 17:30 18:00 18:30 19:00 19:30 20:00 Rinse Cycle 2 20:30 Start Cycle 3 21:00 Process Cycle 2 21:30 22:00 22:30 23:00 23:30 0:00 0:30 1:00 1:30 2:00 Rinse Cycle 3 2:30 Start Cycle 4 3:00 Process Cycle 3 3:30 4:00 4:30 5:00 5:30 6:00 6:30 7:00 7:30 8:00 Rinse Cycle 4 8:30 Process Cycle 4 9:00 Note: Schedule is separated into 30

- minute increments to show activities associated with each c leaning and collection cycle at the two sets of traveling screens.

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 6 Table A4 - 2 . Equipment List. 1. Datasheets printed on waterproof paper 2. Pencils 3. Scales (Electronic and spring) 4. Measuring boards 5. Fish and invertebrate identification keys 6. Buckets and plastic tot es 7. Floodlights and extension cords 8. Calipers 9. Calculator 10. Hardhats 11. Safety Glasses 12. Rubber/latex gloves 13. Clipboard

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 7 Figure A4 - 1. Unit 5 traveling screens.

### Harbor Generating Station

IM&E Draft Report Appendix A4: Impingement Field Sampling Procedure

A4 - 8 Figure A4 - 2. Unit 5 traveling screen collection basket.

# Harbor Generating Station



# Appendix B Model Parameterization

B1. Estimating Total Entrainment B2. Estimating Proportional Entrainment and the ETM Calculations B3. Demographic Model Calculations

### Harbor Generating Station

IM&E Final Report Appen dix B1: Estimating Total Entrainment

B1 - 1 

# Appendix B1 Estimating Total Entrainment The following se ction describes calculations used for assessing entrainment effects at the Harbor Generating Station (HGS). The equations are presented in a general form that is applicable to sample designs that may have differing numbers of stations, sampling periods, or replicates. The HGS entrainment study will sample only one station. While the summation signs over stations are presented in the equations they will be summing over an n of one in the actual calculations and therefore will drop out of the formulas. A general form can be written for summing entrainment over stations at an intake or entrainment site using cycles within a day and days within time periods. Let

i = period 

#  

1, , i N  **** ; j = day within period 

#   1, , i 

### j N  **** 

### ; k = cycle within day 

#   1, , ij 

### k N  **** 

### ; l = station 

#  

1, , ijk 

### l N  **** 

### ;

m = volume at station within cycle 

#   1, , ijkl 

### m N  **** 

### . The total larval entrainment at an intake source can be expressed as

1 1 1 1 N N N ij ijk N i T ijkl ijkl i j k l 

### E V



    

###  

#     (A1)
where
ijkl 

###  = density of larvae at the lth station within the k th cycle on the jth day in the ith time period;
ijkl 

### V 

### = volume of water passing the at the lth station within the k th cycle on the jth day in the ith time period. This summation assumes that stations represent the total intake volume of the power plant. It also assumes that the larval density in the volume of water passing a station is constant over time and

### Harbor Generating Station

IM&E Final Report Appen dix B1: Estimating Total Entrainment

B1 - 2 space over any cycle. An estimate of the total larval entrainment can be made by taking ijkl 

### n samples of the ijkl



### N volumes passing a station as

1 1 1 1 1 

### ˆ N N n

N ij ijk ijkl N i ijkl T ijklm i j k l m

ijkl 

### V E n 

     

###  

#      (A2)

If we also assume that entrainment volume is constant and the same at all stations then

1 1 1 1 1



### 1 ˆ N N n

N ij ijk ijkl N i T ijkl ijklm i j k l m ijkl 

### E V n 

     

###  

#      (A3)
Strata will be defined as the stations and cycles with constant ij 

### N and ijk 

### N . In addition, we sample i 

### n days of the i



### N possibl e during a period so that

1 1 1 1 1

1 1 1 1 1



### 1 1 ˆ
1 1 N N n n ij ijk ijkl N i T i ij ijk ijkl ijklm i j k l m i ij ijk ijkl N N n n ij ijk ijkl N i i ijklm i j k l m

i ij ijk ijkl 

### E N N N V n N N n
V n N N n 

     

     

###  

        

  

    

#           (A4)

where

1 1 1 N N N ij ijk i i ijkl j l k 

### V V

   

###  

#   
If only one day per period is sampled Equation A4 can be expressed as



###  1 1 1 1

1 1 1 

### 1 ˆ
1

N N n ij ijk ijkl N T i ijklm i k l m

ij ijk ijkl N N ij ijk N i ijkl i k l ij ijk 

### E V N N n
V N N 

    

   

###  

  

     

  

    

#        (A5)

with estimated variance



###  

#   **** 

#   2 2 1 1 1 

### 1 ˆ 1

N N ij ijk N ijkl ijkl T i i k l ij ijk ijkl ijkl 

### Var n Var E V N N N n 

   

###    

 

            

#    (A6)

where

### Harbor Generating Station

IM&E Final Report Appen dix B1: Estimating Total Entrainment

B1 - 3 **** 

#   **** 

#    

**** 2

1

1 

### ; 1

. ijkl

ijkl n ijklm ijkl m ijkl ijkl

n ijklm m ijkl ijkl 

### Var n

n   

  

 

### 





 

#  

Estimates of T



### E based on Equation A5 will be used in FH and AEL calculations to estimate annual effects of entrainment on fishes and invertebrates. Equation A6 will underestimate the true variance because it does not include within

- period variance. In practice, we ignore the finite population correction, 

### 1 ijkl ijkl 

### n N  

       because ijkl



### N is large. Estimators similar to Equation A5 and Equation A6 are used for calculati ng survey period estimates of intake and source populations for use in ETM calculations.

### Harbor Generating Station Appendix B2: Estimating Proportional



### IM&E Final Report Entrainment and the ETM Calculations



### B2 - 1 

# Appendix B2 Estimating Proportional Entrainment and the ETM Calculations The empirical transport model (ETM ) is used to estimate the total mortality probability for larvae from power plant entrainment. The estimate is based on periodic estimates of the probability of entrainment mortality based on daily samples. In the following calculations we assume all larvae entrained die. Generally, sampling takes place over the co urse of a year so that larval mortality of various species is estimated. The daily probability of entrainment can be defined as


#   i i 

### abundance of entrained larvae abundance of larvae in source population probability of entrainment in th time period 1, , . i 

### PE i i N 

  **** In turn, the daily probability can be estimated and expressed as

****

**** i i i 

### E PE R  

### (B1)

where

**** i 

### E 

### = estimated abundance of larvae entrained in the ith time period 

#   1, , i N  **** 

### ;

**** i 

### R 

### = estimated abundance of larvae at risk of entrainment from the source population in the i th time period 

#   1, , i N  **** . 

# Estimating Daily Entrainment The estimate of total Harbor Generating Station (HGS) entrainment on day j in period i can be expressed from equation (A4) as

### Harbor Generating Station Appendix B2: Estimating Proportional



### IM&E Final Report Entrainment and the ETM Calculations



### B2 - 2 **** 4 1 3

1 1 1 4 1 3

1 1 1 

### 1 3 1 12 ij ijkl ijklm k l m
ij ijklm k l m 

### E V
V 
   

   

###   
     

#       (B2)

with associated variance

****



#   2

4 1 2 2 1 1 

### 1 3

1 12 ijkl ij ij ij k l ijkl 

### Var E E V S N 

  

###    
 

          

#   (B3) which can be estimated by

** **  

#   2

4 1 2 2 1 1 

### 1 3

1 . 12 ijkl ij ij k l ijkl 

### Var E V s N 

  

###    
 

          

#   (B4)

The finite population correction [i.e., 3 1 ijk 

### N  

       

### ] can be ignored because ijkl 

### N is exceedingly large. Only one day is sampled per p eriod. The period estimated entrainment and variance are



###  4 1 3

1 1 1 

### 1 12 i i ijklm k l m 

### E V 

   

###  
     

#    (B5)

** **  

#   2

4 1 2 2 1 1 

### 1

. 12 ijkl i i k l 

### Var E V s 

  

###  
  

  

#   (B6) 

# Estimating Numbers of Larvae at Risk With the defined and agreed

- upon sources of Los Angeles Harbor (S) larvae, the daily abundance of larvae at risk can be estimated by



###  **** ij S S ij 

### R V    (B7) where S 

### V denotes daily exchanged and static volumes at Los Angeles Harbor (S), and ****  

### denotes an estimate of average density in each respective source water bodies. The variance of Expression B7 can be written as



### 



#   **** 

#   2 

### | | ij ij S S S ij ij 

### Var R R V Var  

  (B8) The individual variances within Formula B8 describe temporal

- spatial variance in density within the source populati on during the day of sampling. Six source water locations are sampled in Los Angeles Harbor. Ideally, tow samples would be collected randomly through time and space during a sampling day over a potential source population. However, practical limitations du e to sampling a large area required a directed and fixed time and location sampling scheme. Our

### Harbor Generating Station Appendix B2: Estimating Proportional



### IM&E Final Report Entrainment and the ETM Calculations



### B2 - 3 source water estimates of population and variance are made for each period using only one day, i.e. ** **  i ij 

### R R  

### and 

###  **** 

#   **** 

#   | i ij ij 

### Var R Var R R  . 

# Period Entrainment and ETM Calculations By dividing estimated period entrainment (B5) by the corresponding source population (B7) an estimate of entrainment mortality can be written as

** ** 

**** i i i 

### E PE R  

### (B9) 

# Variance for the Estimate of i 

# PE The variance for the period estimate of **** i 

### PE 

### can be expressed as



###  

#   ****

**** , i i i i i ij 

### E Var PE PE Var E R

R          . Assuming zero covariance between the entrainment and source and using the delta method (Seber 1982), the variance of an estimator formed from a quotient (like **** i 

### PE 

### ) can be effectively approximated by

2 2 

### ( ) ( ) . A A A B B Var Var A Var B B A B

                       

         

         

    

### The delta method approximation of **** ( ) i 

### Var PE 

### is shown as



###  

#   ****

****
i i i S S



### E Var PE Var V 

 

    

   where by the Delta method can be approximated by



###  **** 

#   ** **  

#  

** **  

#   ****

**** 

#   2 2

2 

### 1

i i i i i i S S S S S S 

### E Var PE Var E Var V

V V

  

   

         

 

  

     (B10)

and is equivalent to

### Harbor Generating Station Appendix B2: Estimating Proportional



### IM&E Final Report Entrainment and the ETM Calculations



### B2 - 4 **** 

#   **** 

#   2 2 2 i i i S S 

### PE CV E CV V   
       

### where



###  **** i S S ij 

### R V    and



###  

#   ** **  

#  

**** 2 

### . Var CV       Regardless of whether the species has a single spawning period per year or mul tiple overlapping spawnings the estimate of total larval entrainment mortality can be expressed by



###  ** **  

#  
1 

### 1 1 N q M i i

i 

### P f PE

 

###    

#  (B11)
where
q 

### = number of days of larval life, and



### ˆ i 

### f = estimated annual frac tion of total larvae hatched during the ith survey period. Formula (B11) is based on the total probability law where 

#   

#    

1 

### . N i i i 

### P A P A B P B

 

###   

#  In the above example, the event A is larval survival and event B is hatching with 

#   P B



### e stimated by **** i 

### f 

### where

** ** 

**** i i T 

### E f E  ,

where 

### 

i 

### E = estimated entrainment for the i th survey period. Then based on the Delta method

### Harbor Generating Station Appendix B2: Estimating Proportional



### IM&E Final Report Entrainment and the ETM Calculations



### B2 - 5 

###  ** **  ****

****

** ** 

** ** 

** **  ** ** 

** **  ****

**** 2 2 2 2 

### ( )

( ) ( )

(1 ) . i i T

i

N i j j i

i T i i T i 

### E Var f Var E

E

Var E E

Var E Var E f f E

E  

###  

                           

    

# 

The estimates of i



### PE and i 

### f and their respective variance estimates can be combined in an estimate of the variance for **** M 

### P 

### following the Delta method (Seber 1982) for variance and covariance as follows:



###  ** **  ** ** 

** **  ****

** ** 

** **  ****

** **  

#   ** ** 

** **  

#  
** **  **** 1

1
2 1
1 2 1

1 2 

### ( ) 1 (1 )

(1 ) ( )(1 ) ( )( (1 ) ) 2 cov , (1 ) (1 ) where
1 cov , N q

M i i i N q

i i i N q i i i N q

i i i i N N q q j i i j i j i

j i j i

T 

### Var P Var f PE

Var f PE Var f PE Var PE f q PE f f PE PE
f f f f Var E

E 









  

###  

        

              
  


   

       

#      

** **  ****



#   ** **  **** 

#   ****

, 

### 1 1 . N g i j

i j j i g i j 

### f f E f f E
 

###     
 
           

# 

### Harbor Generating Station

IM&E Final Report Appendix B3: Demographic Model Calculations

B3 - 1 

# Appendix B3 Demographic Model Calculations Fecundity Hindcasting ( FH ) The estimated total larval entrainment for a species ( **** T 

### E 

### ) was used to estimate the number of breeding females needed to produce the number of larvae en trained. The estimated number of breeding females ( ****

FH 

### ) whose fecundity was equal to the estimated total loss of entrained larvae is calculated as follows:

** ** 

****
1 Tn i i 

### E FH TLF S

 

###  

#  **** 

### (C1) where

****

**** number of larval stages vulnerable to entrain ment, estimated total entrainment, survival rate from eggs to larvae of the th stage, and estimated total life time fecundity for females, e T
i 

### n E S i TLF 





 quivalent to the average number of eggs spawned per female over their reproductive years. 

### Equation C1 is based on the simplified case of a single synchronized spawning by a species. For species with overlapping or continuous spawning, larval abundance would have to be specified by week and age class (i.e., **** ij 

### E 

### ). However, we used the mean size of all larvae entrained to estimate a representative age of larvae, and then estimated a survival rate to this representative age. Two input parameters in Equation C1 that may not be available for many species, and thus may limit the method, are lifetime fecundity ( TLF 

### ) and survival rates ( i 

### S 

### ) from spawning to entrainment. In practice, survival was estimated by either one or several age classes, depending on the data source, to the estimated age at entrainme nt. The expected total lifetime fecundity ( ) E TLF 

### was approximated by modeling a linear survivorship for a female once she reached the age of maturity, and using a constant number of eggs produced per year.

### Harbor Generating Station

IM&E Final Report Appendix B3: Demog raphic Model Calculations

B3 - 2 The number of eggs produced per year was approximated as the average number of eggs per year. Thus

**** 

##   ( )

2 L

M
L

M A

A A L

L M A

L M 

### TLF F A s A dA

A A F dA A A A A F 

 



  

     

#  

where 

##  

s A 

### = survivorship of a female; 

##   F A 

### = eggs produced;

M 

### A 

### = age of maturity; an d

L 

### A 

### = age at death. In other words, 0 0.5 1

0 1 2 3 4 5 6 7 

### A (Age)Survivorships(A)

M 

# A L 

# A

### Harbor Generating Station

IM&E Final Report Appendix B3: Demog raphic Model Calculations

B3 - 3 **** Estimated Total Lifetime Fecundity = Average eggs/year Average number of years of reproductive life

Longevity - Age at maturation Average eggs/year . 2 TLF 

  

   

  

### (C2)

The expected length of reproductive life was approximated as the midpoint between the times of maturation and longevity. The approximation of linear survivorship between these events implies uniform survival. For exploited species such as northern anchovy and sardine, the expected number of years of reproductive life may be much less than predicted using this assumption. Simulation, comparing exponential survival, shows that the calculation of 

###  TLF will be negatively biased for species with short reproductive lifespans, and positively biased for those with longer durations.
The variance of ****

FH 

### was approximated by the Delta m ethod (Seber 1982):

** **  ** **  ** **  

##   **** 

##   2 2 2 2 2 1 

### ˆ ( ) ( ) ( ) ( ) ( ) ( ) n L M T j j L m 

### Var A Var A Var FH FH CV E CV S CV F A A
 

###    

          

       

#  where

****

**** ˆ CV( )= CV of estimated entrainment (estimated by CV(I) when available), CV( ) = CV of estimated survival of eggs and larvae up to entrainment, CV( ) = CV of estimated average annual fe cundity, = a T j

M 

### E S F A ****

ge at maturation, and = age at maturity. L 

### A 

### The behavior of the estimator for FH appears log

- linear, suggesting that an approximate confidence interval can be based on the assumptions that ln( **** FH 

### ) is normally distributed and uses the pivotal quantity

****

** ** 

**** 2 

### ln ln

. ( ) FH FH Z Var FH

FH  

### Harbor Generating Station

IM&E Final Report Appendix B3: Demog raphic Model Calculations

B3 - 4 A 90% confidence interval for FH was estimated by solving for FH and setting Z equal to +/

- 1.645, i.e.

** **  ****

**** 

###  ** ** 

**** 2 2 ( ) ( ) 1.645 1.645



### to . Var FH Var FH FH FH 

### FH e FH e

 



###   

# Adult Equivalent Loss ( AEL)
The AEL approach uses esti mates of the abundance of entrained or impinged organisms to forecast the loss of equivalent numbers of adults. Starting with the number of age class j larvae entrained ( ****

j 

### E 

### ), it is conceptually easy to convert these numbers to an equi valent number of adults lost ( ****

AEL 

### ) at some specified age class from the formula:

** **  ****

1 n j j j 

### AEL E S

 

###  

#  (C3) where

****

**** number of age classes, estimated number of larvae lost in age class , and survival rate for the th age class to a dulthood (Goodyear 1978). j
j 

### n E j S j 



 

### Age

- specific survival rates from larval stage to recruitment into the fishery (through juvenile and early adult stages) must be included in this assessment method. For some commercial species, survival rates are known for adults in the fishery; but for most species, age

- specific larval survivorship has not been well described. When age

- spec ific survival rates from larval stage to recruitment into the fishery were available, AEL was calculated using survival from a representative age of the entrained larvae at HGS. This age was calculated by dividing the average larval length at entrainment ( minus hatch length) by a literature

- based growth rate. Age

- specific survivorship for any interval of time (t) was then calculated following the formula (Ricker 1975)

0 Zt t 

### N e N  

###  

### where

### Harbor Generating Station

IM&E Final Report Appendix B3: Demog raphic Model Calculations

B3 - 5 0

0 

### number of animals in the population at time , number of animals in the population at time 0, (finite survivorship to time ), 2.71828...(base of the natural log), and instantaneou t

t 

### N t N t N S t N
e Z 

 





 s mortality rate. 

### Survivorship to recruitment, to an adult age, was apportioned into several age stages, and AEL was calculated using the total entrainment as

** ** 

1 

### ˆ n T j j 

### AEL E S

 

###  

#  (C4) where

**** number of age classes from entrainment to recruitment and survival rate from the beginning to end of the th age class. j 

### n S j 




### The variance of ****

AEL 

### can be estimated using a Taylor series app roximation (Delta method of Seber 1982) as

** **  ** **  2 2 2 1 

### ˆ ( ) ( ) ( ) n T j j 

### Var AEL AEL CV E CV S

 

###  

      

# 

. (C5) An alternative analysis would be to compare 

###  AEL with the size of the adult population of interest or with fishery harvest data. This method converts numbers of adult losses into fractional loss of the population of interest (e.g., stock assessment). However, information describing adult stocks is limited for many species, and independent field estimates of survival from time of entrainment to adulthood are not available for some species. For some species where such information is unavailable, we can estimate this parameter by assuming a stationary population where an adult female must produce two adults (i.e., one male and one female). Overall survival ( ) T 

### S 

### can then be estimated from total lifetime fecundity ( TLF 

### ) by the quantity

****
**** 2 ˆ ˆ ˆ , T egg larvae adult 

### S S S S TLF     

### which leads to

**** 2 ˆ

.

ˆ ˆ adult egg larvae 

### S TLF S S 

  

### (C6) Substituting Equation 11 into the overall form of the AEL equation where

### Harbor Generating Station

IM&E Final Report Appendix B3: Demog raphic Model Calculations

B3 - 6 **** ˆ ˆ T adult 

### AEL E S   

### (C7) yields

****

** **  **** ˆ 2( ) T egg larva 

### E AEL S S TLF 

  

### where

** **  2 AEL FH  

### . (C8) Without independent adult survival rates and assuming a 50:50 sex ratio, **** AEL 

### and ****

FH 

### are deterministically relate d according to Equation 13, with an associated standard error of

** **  ** **  ( ) 2 ( ). SE AEL SE FH

 

### Equation 13 should be aligned so that the average female age is also the age of recruitment used in computing **** AEL 

### . This alignment is accomplished by solving the simple exponential survival equation (Ricker 1975)

0 ( ) 0 Z t t t 

### N N e   

###   

### by substituting numbers of either equivalent adults or hindcast females, their associated ages, and mortality rates into the equation where,

0 0 

### number of adults at time , number of adults at time , instantaneous rate of natural mortality , and age of hindcast animals ( ) or extrapolated age of animals ( ). t 

### N t N t Z t FH AEL 





 

### This allows for the alignment of ages in either direction such that 2 FH AEL  

### since they are either hindcast or extrapolated to the same age. The estimates of mortality calculated from the AEL and FH approaches can be compared for the same time periods for taxa where independent estimates are available for (1) survival from entrainment to recruitment into the fishery and (2) entrainment back to hatching. These comparisons serve as a method of cross

- validation for the demographic approaches to impact assessment.

### Harbor Generating Station

IM&E Final Report Appendix B3: Demog raphic Model Calculations

B3 - 7 

# Literature Cited Goodyear, C. P. 1978. Entrainment impact estimates using the equivalent adult approach. United States Fish and Wildlife Service, FWS/OBS

- 78/65, Ann Arbor, MI. Ricker, W. E. 1975. Computation and interpretation of bi ological statistics of fish populations. Fish. Res. Board of Canada Bull. 91. 382 pp. Seber, G. A. F. 1982. The Estimation of Animal Abundance and Related Parameters. McMillan. London. 654 p.

# Harbor Generating Station



# Appendix C Entrainment Data

C1. Entrainment Data by Survey C2. Calculated Total Annual Entrainment and Standard Error Data

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 1 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA01 Start Date: 1/10/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 244 390.33 Acanthogobius flavimanus yellowfin goby 130 204.34 Genyonemus lineatus white croaker 104 171.09 Lepidogobius lepidus bay goby 18 28.33 Sciaenidae unid. croakers 16 25.83 larval fish fragment unidentified larval fishes 12 19.76 Gillichthys mirabilis longjaw mudsucker 11 18.57 Clinocottus analis wooly sculpin 9 15.22 Paralichthys californicus California halibut 2 3.40 P leuronichthys guttulatus diamond turbot 2 3.35 Hypsoblennius spp . combtooth blennies 2 2.90 Gibbonsia spp . clinid kelpfishes 1 2.05 Leptocottus armatus Pacific staghorn sculpin 1 1.92 larval/post

- larval fish unid. larval fishes 1 1.71 Gobiesox spp . clingfishes 1 1.68 Gobiesocidae unid. clingfishes 1 1.48 Total Fishes: 555 Eggs Genyonemus lineatus (eggs) white croaker eggs 262 447.99 Citharichthys spp. (eggs) sanddab eggs 71 111.95 Paralichthys californicus (eggs) California halibut eggs 37 60.27 fish eggs unid. unidentified fish eggs 15 26.08 Pleuronichthys spp. (eggs) turbot eggs 10 17.14 Total Eggs: 395 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 15 24.73 Pinnixa spp. (megalops) pea crabs megalops 3 4.45 Grapsidae unid. (megalops) shore crab megalops 1 1.75 Total Target Invertebrates: 19

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 2 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA02 Start Date: 1/23/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 154 368.16 Acanthogobius flavimanus yellowfin goby 144 342.99 Genyonemus lineatus white croaker 19 46.96 Lepidogobius lepidus bay goby 8 20.70 Sciaenidae unid. croakers 2 5.16 Gobiesox spp . clingfis hes 1 3.14 larval/post

- larval fish unid. larval fishes 1 3.14 larval fish fragment unidentified larval fishes 1 3.05 Paralichthys californicus California halibut 1 2.58 Pleuronichthys guttulatus diamond turbot 1 2.58 Ruscarius creaseri roughcheek scul pin 1 2.58 Total Fishes: 333 Eggs Genyonemus lineatus (eggs) white croaker eggs 498 1,306.88 fish eggs unid. unidentified fish eggs 128 324.39 Paralichthys californicus (eggs) California halibut eggs 25 59.09 Pleuronichthys spp. (eggs) turbot eggs 1 2 28.85 Paralichthyidae unid. (eggs) sand flounder eggs 8 20.68 Citharichthys spp. (eggs) sanddab eggs 7 15.85 Total Eggs: 678 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 7 18.51 unidentified crab (megalops) unidentified crab m egalops 4 8.87 Pinnixa spp. (megalops) pea crabs megalops 1 2.86 Total Target Invertebrates: 12

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 3 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA02 Start Date: 1/23/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 83 145.43 Genyonemus lineatus white croaker 88 138.63 Acanthogobius flavimanus yellowfin goby 77 119.88 Lepidogobius lepidus bay goby 74 117.32 Sciaenidae unid. croakers 7 9. 51 Ruscarius creaseri roughcheek sculpin 5 8.66 Clinocottus analis wooly sculpin 4 6.51 larval/post

- larval fish unid. larval fishes 3 5.51 Icelinus spp . sculpins 3 4.69 Myctophidae unid. lanternfishes 2 3.79 Hypsoblennius spp . combtooth blennies 2 3. 26 larval fish fragment unidentified larval fishes 2 3.12 Pleuronichthys guttulatus diamond turbot 1 1.89 Sebastes spp . rockfishes 1 1.89 Merluccius productus Pacific hake 1 1.41 Engraulis mordax northern anchovy 1 1.36 Paralichthys californicus Cali fornia halibut 1 1.36 Gibbonsia spp . clinid kelpfishes 1 1.32 Total Fishes: 356 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 95 150.17 Pinnixa spp. (megalops) pea crabs megalops 9 13.57 unidentified crab (megalops) unidentified crab megalops 8 12.80 Hemigrapsus spp. (megalops) shore crab megalops 1 1.41 Loligo opalescens market squid 1 1.36 Grapsidae unid. (megalops) shore crab megalops 1 1.32 Total Target Invertebrates: 115

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 4 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA02 Start Date: 1/23/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 37 195.04 Gobiidae unid. gobies 9 46.05 Ruscarius creaseri roughcheek sculpin 3 18.06 Atherinopsis californiensis jacksmelt 3 12.94 Acanthogobius flavimanus yellowfin goby 2 12.48 Pleuronichthys guttulatus diamond turbot 2 8.98 Lepidogobius lepidus bay goby 1 7.86 Paralichthys californicus California halibut 1 7.86 Clinocottus analis wooly sculpin 1 6.24 Orthonopias triacis snubnose sculpin 1 5.02 Stenobrachius leucopsarus northern lampfish 1 5.02 Atherinops affinis topsmelt 1 3.96 Sciaenidae unid. croakers 1 3.96 Total Fishes: 63 Target Invertebrates Loligo opalescens market squid 12 62.89 Pugettia spp. (megalops) kelp crabs megalops 5 32.56 Total Target Invertebrates: 17

### Harbor Generating Statio n

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C1 - 5 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA02 Start Date: 1/23/2006 S tations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 163 408.08 Gobiidae unid. gobies 19 42.83 Lepidogobius lepidus bay goby 18 42.56 Sciaenidae unid. croakers 13 27.77 Paralichthys calif

ornicus California halibut 8 23.90 Ruscarius creaseri roughcheek sculpin 6 14.61 larval fish fragment unidentified larval fishes 5 13.41 Stichaeidae unid. pricklebacks 2 5.33 Clinocottus analis wooly sculpin 1 3.35 Gibbonsia spp . clinid kelpfishes 1 3 .35 Scorpaenichthys marmoratus cabezon 1 3.35 Rhinogobiops nicholsi blackeye goby 1 3.06 Atherinopsis californiensis jacksmelt 1 2.43 Pleuronichthys guttulatus diamond turbot 1 2.43 Stenobrachius leucopsarus northern lampfish 1 2.43 Hypsoblennius spp . combtooth blennies 1 2.27 Gobiesox spp . clingfishes 1 2.17 Zaniolepis spp . combfishes 1 2.17 Citharichthys sordidus Pacific sanddab 1 1.80 larval/post

- larval fish unid. larval fishes 1 1.80 Total Fishes: 246 Target Invertebrates Pugettia spp. (me galops) kelp crabs megalops 57 124.15 Loligo opalescens market squid 14 36.75 unidentified crab (megalops) unidentified crab megalops 8 17.35 Pinnixa spp. (megalops) pea crabs megalops 7 17.03 Paguridae unid. (megalops) hermit crab megalops 1 2.27 Porcellanidae unid. (megalops) porcelain crab megalops 1 2.27 Total Target Invertebrates: 88

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C1 - 6 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA03 Start Date: 2/7/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Acanthogobius flavimanus yellowfin goby 376 1,022.76 Gobiidae unid. gobies 308 841.71 Genyonemus lineatus white croaker 161 448.72 Lepidogobius lepidus bay goby 35 95.48 Paralichthys californicus California halibut 7 18.13 Gillichthys mirabilis longjaw mudsucker 5 13.84 Paraclinus spp . clinid 3 8.94 Gobiesox spp . clingfishes 3 7.99 Sciaenidae unid. croakers 1 2.98 Citharichthys sordidus Pacific sanddab 1 2.88 Hypsoblennius spp . combtooth blennies 1 2.78 larval fish fragment unidentified larval fishes 1 2.66 larval/post

- larval fish unid. larval fishes 1 2.55 Clinocottus analis wooly sculpin 1 2.30 Total Fishes: 904 Eggs Genyonemus lineatus (eggs) white croaker eggs 532 1,492.88 fish eggs unid. unide ntified fish eggs 117 318.61 Paralichthys californicus (eggs) California halibut eggs 77 223.82 Pleuronichthys spp. (eggs) turbot eggs 20 54.95 Citharichthys spp. (eggs) sanddab eggs 7 19.61 Paralichthyidae unid. (eggs) sand flounder eggs 5 17.08 Total Eggs: 758 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 115 326.08 Pinnixa spp. (megalops) pea crabs megalops 6 17.25 Majidae unid. (megalops) spider crab megalops 5 14.80 unidentified crab (megalops) unidentified crab megalops 5 12.80 Paguridae unid. (megalops) hermit crab megalops 1 3.12 Total Target Invertebrates: 132

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C1 - 7 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA04 Start Date: 2/21/2006 Stations: E1 Mean Concentrati on Taxon Common Name Count (#/1,000m 3 ) Fishes Acanthogobius flavimanus yellowfin goby 125 360.91 Genyonemus lineatus white croaker 92 284.52 Gobiidae unid. gobies 31 101.10 Lepidogobius lepidus bay goby 10 30.39 larvae, unidentified yolksac unide ntified yolksac larvae 7 25.00 larval fish fragment unidentified larval fishes 6 20.31 Citharichthys stigmaeus speckled sanddab 2 7.42 Sciaenidae unid. croakers 2 7.42 Rhinogobiops nicholsi blackeye goby 2 6.69 Pleuronectiformes unid. flatfishes 2 6.6 0 Paralichthys californicus California halibut 2 6.50 Ruscarius creaseri roughcheek sculpin 2 5.79 Gobiesox spp . clingfishes 1 5.48 Total Fishes: 284 Eggs fish eggs unid. unidentified fish eggs 1,263 3,764.61 Genyonemus lineatus (eggs) white croaker eggs 975 3,137.41 Paralichthyidae unid. (eggs) sand flounder eggs 82 266.29 Citharichthys spp. (eggs) sanddab eggs 29 98.06 Pleuronichthys spp. (eggs) turbot eggs 7 24.55 Engraulidae unid. (eggs) anchovy eggs 6 17.36 Total Eggs: 2,362 Target Inver tebrates Pugettia spp. (megalops) kelp crabs megalops 55 183.51 Majidae unid. (megalops) spider crab megalops 5 16.01 Pinnixa spp. (megalops) pea crabs megalops 5 13.88 unidentified crab (megalops) unidentified crab megalops 2 6.93 Grapsidae unid. (me galops) shore crab megalops 1 3.71 Total Target Invertebrates: 68

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C1 - 8 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA04 Start Date: 2/21/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 206 338.62 Acanthogobius flavimanus yellowfin goby 216 316.14 Gobiidae unid. gobies 55 91.04 larval fish fragment unidentified larval fishes 31 51.83 Lepidogobius lepidus bay goby 27 43.32 larv ae, unidentified yolksac unidentified yolksac larvae 13 22.39 Sciaenidae unid. croakers 12 20.84 Hypsoblennius spp . combtooth blennies 7 12.24 Ruscarius creaseri roughcheek sculpin 4 6.83 Cottidae unid. sculpins 4 6.37 Paralichthys californicus California halibut 3 4.88 Bathymasteridae unid. ronquils 2 3.38 larval/post

- larval fish unid. larval fishes 1 1.70 Atherinops affinis topsmelt 1 1.63 Citharichthys sordidus Pacific sanddab 1 1.63 Pleuronichthys guttulatus diamond turbot 1 1.63 Stenobrachiu s leucopsarus northern lampfish 1 1.63 Merluccius productus Pacific hake 1 1.51 Rhinogobiops nicholsi blackeye goby 1 1.51 Total Fishes: 587 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 92 150.82 Pinnixa spp. (megalops) pea crab s megalops 10 17.19 Hemigrapsus spp. (megalops) shore crab megalops 8 13.97 Majidae unid. (megalops) spider crab megalops 5 8.29 unidentified crab (megalops) unidentified crab megalops 4 5.53 Total Target Invertebrates: 119

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C1 - 9 Harbor Generating Sta tion Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA04 Start Date: 2/21/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 107 497.93 Gobiidae unid. gobies 14 61.98 larval fish fragment unidentified larval fishes 7 32.53 larvae, unidentified yolksac unidentified yolksac larvae 4 23.35 Acanthogobius flavimanus yellowfin goby 4 11.06 Bathymasteridae unid. ronquils 2 9.50 Clinocottus analis wooly sculpin 1 6.73 larval fish

- damaged unidentified larval fishes 1 6.73 Rhinogobiops nicholsi blackeye goby 1 5.84 Cottidae unid. sculpins 1 5.11 Paralichthys californicus California halibut 1 5.11 Sciaenidae unid. croakers 1 5.11 Artedius lateralis smoothhead sculpin 1 2.76 Chaenopsidae unid. tube blennies 1 2.76 Total Fishes: 146 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 14 59.07 Pinnixa spp. (megalops) pea crabs megalops 10 39.36 Majidae unid. (megalops) spider crab megalops 4 11.06 To tal Target Invertebrates: 28

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C1 - 10 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA04 Start Date: 2/21/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus li neatus white croaker 162 448.08 larval fish fragment unidentified larval fishes 42 120.28 Ruscarius creaseri roughcheek sculpin 10 26.89 Lepidogobius lepidus bay goby 11 25.04 Gobiidae unid. gobies 9 24.15 larvae, unidentified yolksac unidentified yol ksac larvae 8 21.33 Rhinogobiops nicholsi blackeye goby 7 19.82 Bathymasteridae unid. ronquils 6 16.26 Cottidae unid. sculpins 6 15.68 Zaniolepis frenata shortspine combfish 4 11.93 Labrisomidae unid. labrisomid blennies 2 5.74 Sebastes spp . rockfish es 2 5.09 Hypsoblennius spp . combtooth blennies 2 4.97 Sciaenidae unid. croakers 2 4.39 Artedius harringtoni scalyhead sculpin 1 4.04 Seriphus politus queenfish 1 4.04 Orthonopias triacis snubnose sculpin 1 2.99 Acanthogobius flavimanus yellowfin gob y 1 2.92 Gillichthys mirabilis longjaw mudsucker 1 2.75 Oligocottus spp . sculpins 1 2.75 Stenobrachius leucopsarus northern lampfish 1 2.75 Chaenopsidae unid. tube blennies 1 2.48 Gobiesox spp . clingfishes 1 2.48 Pleuronichthys guttulatus diamond turbot 1 2.48 larval/post

- larval fish unid. larval fishes 1 2.17 Clinocottus analis wooly sculpin 1 1.91 Total Fishes: 285 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 24 69.16 Pinnixa spp. (megalops) pea crabs megalops 8 17.22 unidentified crab (megalops) unidentified crab megalops 5 13.57 Paguridae unid. (megalops) hermit crab megalops 3 8.76 Majidae unid. (megalops) spider crab megalops 2 4.39 Total Target Invertebrates: 42

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C1 - 11 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA05 Start Date: 3/8/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Acanthogobius flavimanus yellowfin goby 1,121 4,384.63 Gobiidae unid. gobies 321 1,145.63 Genyonemus lineatus white croaker 83 316.93 Lepidogobius lepidus bay goby 11 37.82 larval fish fragment unidentified larval fishes 6 16.34 Gobiesox spp . clingfishes 3 13.03 Gillichthys mirabilis longjaw mudsucker 4 10.59 Gibbonsia spp . clinid kelpfishes 1 7.50 Leptocottus armatus Pacific staghorn sculpin 1 7.50 larval fish

- damaged unidentified larval fishes 2 6.78 larvae, unidentified yolksac unidentified yolksac larvae 2 5.82 Bathymasteridae unid. ronquils 1 2.73 Paralichthys californicus California hal ibut 1 2.65 Total Fishes: 1,557 Eggs Sciaenidae unid. (eggs) croaker eggs 680 2,057.21 fish eggs unid. unidentified fish eggs 531 1,538.01 Genyonemus lineatus (eggs) white croaker eggs 293 925.62 Paralichthyidae unid. (eggs) sand flounder eggs 214 6 12.63 Citharichthys spp. (eggs) sanddab eggs 80 240.99 Pleuronichthys spp. (eggs) turbot eggs 19 59.95 Engraulidae unid. (eggs) anchovy eggs 3 10.22 Total Eggs: 1,820 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 57 169.02 Pinni xa spp. (megalops) pea crabs megalops 7 19.59 unidentified crab (megalops) unidentified crab megalops 7 19.16 Majidae unid. (megalops) spider crab megalops 3 9.50 Pachygrapsus crassipes (megalops) striped shore crab megalops 1 2.91 Hemigrapsus nudus (megalops) purple shore crab megalops 1 2.88 Total Target Invertebrates: 76

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C1 - 12 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA06 Start Date: 3/20/2006 Stations: E1 Mean Concentration Taxon Common Name Coun t (#/1,000m 3 ) Fishes Gobiidae unid. gobies 199 630.28 Acanthogobius flavimanus yellowfin goby 76 246.51 Genyonemus lineatus white croaker 21 71.36 Engraulis mordax northern anchovy 9 28.23 Lepidogobius lepidus bay goby 9 25.18 Leptocottus armatus Pacific staghorn sculpin 5 16.17 Sciaenidae unid. croakers 2 6.76 Orthonopias triacis snubnose sculpin 2 6.09 Gillichthys mirabilis longjaw mudsucker 2 5.96 Icelinus spp . sculpins 2 5.96 Merluccius productus Pacific hake 1 3.71 Gobiesox spp . clingfi shes 1 3.59 larvae, unidentified yolksac unidentified yolksac larvae 1 3.17 Cottidae unid. sculpins 1 2.38 Total Fishes: 331 Eggs fish eggs unid. unidentified fish eggs 686 2,244.82 Sciaen. / Paralich. / Labridae (eggs) fish eggs 220 699.98 Sciaeni dae unid. (eggs) croaker eggs 47 175.54 Pleuronichthys spp. (eggs) turbot eggs 8 25.40 Paralichthyidae unid. (eggs) sand flounder eggs 5 18.61 Engraulidae unid. (eggs) anchovy eggs 2 6.60 Total Eggs: 968 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 38 129.42 Majidae unid. (megalops) spider crab megalops 6 20.43 Pinnixa spp. (megalops) pea crabs megalops 5 17.99 Hemigrapsus spp. (megalops) shore crab megalops 1 3.59 Loligo opalescens market squid 1 3.59 Total Target Invertebr ates: 51

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C1 - 13 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA06 Start Date: 3/20/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 114 246.36 Genyonemus lineatus white croaker 64 132.25 Acanthogobius flavimanus yellowfin goby 45 97.88 Lepidogobius lepidus bay goby 23 44.95 larval fish

- damaged unidentified larval fishes 8 17.61 Engraulis mordax northern anchovy 6 13.42 Sciaenidae unid. cro akers 5 10.73 Gobiesox spp . clingfishes 4 8.62 Engraulidae unid. anchovies 4 7.64 Icelinus spp . sculpins 3 7.13 Stenobrachius leucopsarus northern lampfish 3 6.66 larval fish fragment unidentified larval fishes 3 5.67 Cottidae unid. sculpins 3 5.62 Bathymasteridae unid. ronquils 2 3.62 Clinocottus analis wooly sculpin 2 3.11 Bathylagus ochotensis popeye blacksmelt 1 2.37 Sebastes spp . rockfishes 1 2.37 Myctophidae unid. lanternfishes 1 2.34 Parophrys vetulus English sole 1 2.26 Ruscarius crease ri roughcheek sculpin 1 2.26 Chaenopsidae unid. tube blennies 1 2.10 Pleuronectiformes unid. flatfishes 1 2.05 Atherinopsidae unid. silversides 1 1.52 larvae, unidentified yolksac unidentified yolksac larvae 1 1.52 Typhlogobius californiensis blind go by 1 1.52 Total Fishes: 299 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 66 135.94 Majidae unid. (megalops) spider crab megalops 28 56.55 Pinnixa spp. (megalops) pea crabs megalops 14 26.64 unidentified crab (megalops) unidentif ied crab megalops 5 10.23 Paguridae unid. (megalops) hermit crab megalops 3 6.19 Hemigrapsus spp. (megalops) shore crab megalops 2 3.88 Cancer gracilis (megalops) slender crab megalops 1 2.10 Diogenidae (megalops) left

- handed hermit crabs 1 2.10 Loligo opalescens market squid 1 2.02 Total Target Invertebrates: 121

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C1 - 14 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA06 Start Date: 3/20/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1, 000m 3 ) Fishes Gobiidae unid. gobies 14 71.03 Genyonemus lineatus white croaker 13 68.95 Gobiesox spp . clingfishes 4 23.19 Icelinus spp . sculpins 3 17.36 Sciaenidae unid. croakers 3 13.76 Artedius lateralis smoothhead sculpin 2 13.40 Bathylagus o chotensis popeye blacksmelt 2 13.39 Pleuronectidae unid. righteye flounders 2 9.80 Lepidogobius lepidus bay goby 1 6.70 Parophrys vetulus English sole 1 6.70 Zaniolepis frenata shortspine combfish 1 6.70 Leuroglossus stilbius California smoothtongue 1 6.69 Clinocottus analis wooly sculpin 1 5.84 Gibbonsia spp . clinid kelpfishes 1 5.84 Orthonopias triacis snubnose sculpin 1 5.84 Acanthogobius flavimanus yellowfin goby 1 3.96 Engraulis mordax northern anchovy 1 3.96 Merluccius productus Pacific hake 1 3.96 Paralichthys californicus California halibut 1 3.96 Rhinogobiops nicholsi blackeye goby 1 3.96 Total Fishes: 55 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 14 79.52 Majidae unid. (megalops) spider crab megalops 10 45.2 5 Paguridae unid. (megalops) hermit crab megalops 3 17.35 Pinnixa spp. (megalops) pea crabs megalops 3 11.89 unidentified crab (megalops) unidentified crab megalops 1 6.70 Hemigrapsus spp. (megalops) shore crab megalops 1 3.96 Total Target Invertebrat es: 32

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C1 - 15 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA06 Start Date: 3/20/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 185 544.92 Sciaenidae unid. croakers 50 147.45 Gobiidae unid. gobies 22 74.11 Stenobrachius leucopsarus northern lampfish 18 51.71 Sebastes spp . rockfishes 10 31.22 larval fish fragment unidentified larval fishes 11 30.44 Rhinogobiops nicholsi blackeye g oby 7 22.37 larval fish

- damaged unidentified larval fishes 7 19.39 Orthonopias triacis snubnose sculpin 5 16.11 Oxylebius pictus painted greenling 5 14.97 Lepidogobius lepidus bay goby 5 14.46 larvae, unidentified yolksac unidentified yolksac larvae 5 14.36 Pleuronectidae unid. righteye flounders 4 13.76 Artedius lateralis smoothhead sculpin 4 13.38 Ruscarius creaseri roughcheek sculpin 5 12.66 Cottidae unid. sculpins 4 12.48 Engraulis mordax northern anchovy 4 11.79 Hypsoblennius spp . combtooth blennies 3 10.62 Acanthogobius flavimanus yellowfin goby 3 10.10 Bathymasteridae unid

. ronquils 3 9.20 Typhlogobius californiensis blind goby 3 8.82 Leuroglossus stilbius California smoothtongue 3 8.20 Clinocottus analis wooly sculpin 3 7.68 Paroph rys vetulus English sole 2 7.67 Gibbonsia spp . clinid kelpfishes 2 6.84 Atherinopsis californiensis jacksmelt 3 6.77 Gobiesox spp . clingfishes 3 6.65 Icelinus spp . sculpins 2 6.33 Paralichthyidae unid. sand flounders 2 6.15 Labrisomidae unid. labriso mid blennies 1 3.84 Scorpaenichthys marmoratus cabezon 1 3.77 Diaphus theta California headlight fish 1 3.01 Zaniolepis frenata shortspine combfish 1 3.01 Citharichthys spp . sanddabs 1 2.56 larval/post

- larval fish unid. larval fishes 1 2.56 Leuroglos sus spp . smoothtongues 1 2.56 Paralichthys californicus California halibut 1 2.56 Bathylagus ochotensis popeye blacksmelt 1 2.43 Hexagrammidae unid. greenlings 1 2.43 Merluccius productus Pacific hake 1 1.66 Total Fishes: 394 (continued)

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C1 - 16 Harb or Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA06 Start Date: 3/20/2006 Stations: H5 - H6 (continued) Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 51 139.63 Majidae unid. (megalops) spider crab megalops 27 68.63 Paguridae unid. (megalops) hermit crab megalops 10 25.72 Pinnixa spp. (megalops) pea crabs megalops 3 8.84 Hemigrapsus spp. (megalops) shore crab megalops 3 7.81 Diogenidae (megalops) left

- handed hermit crabs 2 4.74 Loligo opalescens market squid 1 2.43 Cancer productus (megalops) red rock crab megalops 1 1.66 Cancer spp. (megalops) cancer crabs megalops 1 1.66 Total Target Invertebrates: 99

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C1 - 17 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA07 Start Date: 4/3/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 583 1,752.83 Genyonemus lineatus white croaker 271 813.48 Acanthogobius flavimanus yellowfin goby 76 215.63 Sciaenidae unid. croakers 25 76.62 Lepidogobius lepidus bay goby 11 32.91 larval fish fragment unidentified larval fishes 9 29.32 larvae, unidentified yolksac unidentified yolksac larvae 7 20.4 4 larval fish

- damaged unidentified larval fishes 3 10.56 Gibbonsia spp . clinid kelpfishes 2 6.24 Sardinops sagax Pacific sardine 2 5.23 Gillichthys mirabilis longjaw mudsucker 2 5.10 Girella nigricans opaleye 1 3.49 Icelinus spp . sculpins 1 3.48 C lupeidae unid. herrings 1 3.12 Hypsoblennius spp . combtooth blennies 1 3.12 Engraulidae unid. anchovies 1 2.11 Engraulis mordax northern anchovy 1 2.11 Gobiesox spp . clingfishes 1 2.11 Total Fishes: 998 Eggs fish eggs unid. unidentified fish eggs 7 82 2,335.47 Paralichthyidae unid. (eggs) sand flounder eggs 581 1,779.17 Sciaenidae unid. (eggs) croaker eggs 519 1,600.91 Pleuronichthys spp. (eggs) turbot eggs 24 73.85 Citharichthys spp. (eggs) sanddab eggs 5 15.77 Engraulidae unid. (eggs) anchovy eggs 5 15.48 Total Eggs: 1,916 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 34 103.22 Pinnixa spp. (megalops) pea crabs megalops 22 51.77 Paguridae unid. (megalops) hermit crab megalops 6 20.64 Hemigrapsus spp. (megalops) shore crab megalops 6 18.75 Pinnotheres spp. (megalops) pea crab megalops 3 8.43 Majidae unid. (megalops ) spider crab megalops 2 6.69 Diogenidae (megalops) left

- handed hermit crabs 1 3.49 unidentified crab (megalops) unidentified crab megalops 1 3.49 Lolig o opalescens market squid 1 2.11 Total Target Invertebrates: 76

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C1 - 18 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA08 Start Date: 4/17/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000 m 3 ) Fishes Gobiidae unid. gobies 346 1,057.06 Genyonemus lineatus white croaker 114 342.73 Lepidogobius lepidus bay goby 23 69.22 Engraulis mordax northern anchovy 15 44.21 Sciaenidae unid. croakers 10 32.48 Gillichthys mirabilis longjaw mudsucker 5 14.47 Icelinus spp . sculpins 5 13.25 Icelinus quadriseriatus yellowchin sculpin 4 11.08 larval fish fragment unidentified larval fishes 3 9.46 Hypsoblennius spp . combtooth blennies 3 7.83 Gobiesox spp . clingfishes 2 6.24 larval fish

- damaged uni dentified larval fishes 2 6.03 Paralichthys californicus California halibut 2 5.89 larvae, unidentified yolksac unidentified yolksac larvae 2 5.63 Leptocottus armatus Pacific staghorn sculpin 1 3.81 Pleuronichthys verticalis hornyhead turbot 1 3.42 Ac anthogobius flavimanus yellowfin goby 1 3.05 Pleuronectidae unid. righteye flounders 1 3.05 Bathylagus ochotensis popeye blacksmelt 1 2.61 Total Fishes: 541 Eggs fish eggs unid. unidentified fish eggs 751 2,262.31 Sciaenidae unid. (eggs) croaker egg s 632 1,901.23 Paralichthyidae unid. (eggs) sand flounder eggs 154 457.96 Pleuronichthys spp. (eggs) turbot eggs 8 22.14 Engraulidae unid. (eggs) anchovy eggs 3 8.26 Citharichthys spp. (eggs) sanddab eggs 2 5.89 Total Eggs: 1,550 Target Invertebrate s Pugettia spp. (megalops) kelp crabs megalops 90 261.39 Pinnixa spp. (megalops) pea crabs megalops 40 116.01 Majidae unid. (megalops) spider crab megalops 21 61.93 Paguridae unid. (megalops) hermit crab megalops 8 24.25 Grapsidae unid. (megalops) sho re crab megalops 2 6.42 Cancer antennarius (megalops) brown rock crab megalops 2 6.15 Loligo opalescens market squid 2 5.42 Hemigrapsus spp. (megalops) shore crab megalops 1 2.82 unidentified crab (megalops) unidentified crab megalops 1 2.61 Total Tar get Invertebrates: 167

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 19 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA08 Start Date: 4/17/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 560 1,006.34 Gobiidae unid. gobies 167 318.25 Engraulidae unid. anchovies 27 52.48 Sciaenidae unid. croakers 20 36.32 Engraulis mordax northern anchovy 19 36.01 Gillichthys mirabilis longjaw mudsucker 16 30.94 Hypsoblennius spp . combtoo th blennies 14 21.64 Chitonotus / Icelinus sculpins 9 18.17 Gobiesox spp . clingfishes 10 16.24 Bathymasteridae unid. ronquils 4 8.72 Parophrys vetulus English sole 4 8.45 Atherinopsidae unid. silversides 3 6.01 Icelinus quadriseriatus yellowchin scul pin 3 5.29 Seriphus politus queenfish 2 3.17 Paralichthys californicus California halibut 1 2.37 Pleuronectidae unid. righteye flounders 1 2.37 Merluccius productus Pacific hake 1 1.97 Pleuronectiformes unid. flatfishes 1 1.92 Pleuronichthys spp . turbots 1 1.82 Lyopsetta exilis slender sole 1 1.66 Orthonopias triacis snubnose sculpin 1 1.66 Chitonotus pugetensis roughback sculpin 1 1.54 Sebastes spp . rockfishes 1 1.21 Total Fishes: 867 Target Invertebrates Pugettia spp. (megalops) kelp crabs m egalops 180 310.24 Pinnixa spp. (megalops) pea crabs megalops 106 171.38 Majidae unid. (megalops) spider crab megalops 43 76.85 Paguridae unid. (megalops) hermit crab megalops 18 29.93 Grapsidae unid. (megalops) shore crab megalops 15 25.62 Cancer antennarius (megalops) brown rock crab megalops 11 23.11 unidentified crab (megalops) unidentified crab megalops 9 17.66 Pinnotheres spp. (megalops) pea crab megalops 8 13.45 Loligo opalescens market squid 6 11.03 Lophopanopeus spp. (megalops) black

- clawe d crab megalops 2 4.73 Brachyura unid. (megalops) unidentified crab megalops 1 1.92 Hemigrapsus spp. (megalops) shore crab megalops 1 1.92 Cancer anthonyi (megalops) yellow crab megalops 1 1.55 Cancer spp. (megalops) cancer crabs megalops 1 1.54 Total Target Invertebrates: 402

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 20 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA08 Start Date: 4/17/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 242 1,445.78 Gobiidae unid. gobies 32 193.23 Engraulidae unid. anchovies 20 117.94 Engraulis mordax northern anchovy 6 38.41 Sciaenidae unid. croakers 5 31.72 Parophrys vetulus English sole 3 20.41 Artedius lateralis smoothhead sculpin 2 13.72 Cottidae unid. sculpins 2 12.37 Stenobrachius leucopsarus northern lampfish 2 11.36 Lyopsetta exilis slender sole 1 7.03 Atherinops affinis topsmelt 1 5.68 Bathymasteridae unid. ronquils 1 5.68 Hypsoblennius spp . combtooth blennies 1 5.68 Merluccius productus Pacific hake 1 4.62 Total Fishes: 319 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 145 874.41 Pinnixa spp. (megalops) pea crabs megalops 51 278.59 Cancer antennarius (megalops) brown rock crab megalops 36 232.16 Majidae unid. (megalops) spider crab megalops 20 131.31 Grapsidae unid. (megalops) shore crab megalops 11 70.47 unidentified crab (megalops) unidentified crab megalops 9 48.49 Brachyura unid. (megalops) unidentified crab megalops 7 34.43 Loligo opale scens market squid 6 33.94 Paguridae unid. (megalops) hermit crab megalops 5 28.99 Cancer anthonyi (megalops) yellow crab megalops 3 13.87 Hemigrapsus spp. (megalops) shore crab megalops 2 13.72 Pinnotheres spp. (megalops) pea crab megalops 2 11.65 Lo phopanopeus spp. (megalops) black

- clawed crab megalops 2 9.25 Diogenidae (megalops) left

- handed hermit crabs 1 6.69 Pachycheles rudis (megalops) thickclaw porcelain crab 1 4.62 Total Target Invertebrates: 301

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 21 Harbor Generating Station Entrainm ent Study - Fish and Invertebrate Abundances Survey: HGSEA08 Start Date: 4/17/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 1,165 2,619.09 Engraulidae unid. anchovies 220 385.4 1 Sciaenidae unid. croakers 62 166.12 Gobiidae unid. gobies 42 97.23 Orthonopias triacis snubnose sculpin 26 73.81 Engraulis mordax northern anchovy 21 53.38 Parophrys vetulus English sole 16 40.66 Ruscarius creaseri roughcheek sculpin 14 33.10 Hyps oblennius spp . combtooth blennies 14 29.92 Artedius lateralis smoothhead sculpin 9 26.01 Bathymasteridae unid. ronquils 10 23.49 larval fish

- damaged unidentified larval fishes 9 23.33 Gillichthys mirabilis longjaw mudsucker 8 15.37 Seriphus politus queenfish 6 14.31 Paralichthys californicus California halibut 5 13.67 larval fish fragment unidentified larval fishes 7 13.05 Merluccius productus Pacific hake 4 10.80 Clinocottus analis wooly sculpin 4 10.44 Gobiesox spp . clingfishes 4 9.87 Pleuron ichthys verticalis hornyhead turbot 4 9.31 Pleuronichthys spp . turbots 4 9.29 Stenobrachius leucopsarus northern lampfish 4 8.87 larvae, unidentified yolksac unidentified yolksac larvae 3 8.68 Oligocottus / Clinocottus sculpins 3 8.59 Leuroglossus sti lbius California smoothtongue 3 8.45 Chitonotus / Icelinus sculpins 3 8.11 Cottidae unid. sculpins 3 6.83 Labrisomidae unid. labrisomid blennies 3 6.54 Zaniolepis spp . combfishes 2 5.76 Rhinogobiops nicholsi blackeye goby 2 5.27 Isopsetta isolepis butter sole 2 5.01 Sardinops sagax Pacific sardine 2 5.01 Icelinus quadriseriatus yellowchin sculpin 2 4.70 Pleuronectiformes unid. flatfishes 2 4.70 Lyopsetta exilis slender sole 2 4.58 Pleuronectidae unid. righteye flounders 2 3.12 Sebastes spp . rockfishes 1 2.88 Oxylebius pictus painted greenling 1 2.63 Gibbonsia spp . clinid kelpfishes 1 2.51 Pleuronichthys guttulatus diamond turbot 1 2.51 Pleuronichthys ritteri spotted turbot 1 2.51 Total Fishes: 1,697 (continued)

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 22 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA08 Start Date: 4/17/2006 Stations: H5 - H6 (continued) Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 582 1,213.76 Cancer antennarius (megalops) brown rock crab megalops 215 539.41 Majidae unid. (megalops) spider crab megalops 153 358.43 Pinnixa spp. (megalops) pea crabs megalops 162 333.91 unidentified crab (megalops) unidentified crab megalops 49 140.10 Paguridae unid. (megalops) hermit crab megalops 56 137.18 Pinnotheres spp. (megalops) pea crab megalops 55 128.46 Grapsidae unid. (megalops) shore crab megalops 40 88.15 Pachycheles rudis (megalops) thickclaw porcelain crab 12 28.38 Cancer spp. (meg alops) cancer crabs megalops 13 27.84 Cancer anthonyi (megalops) yellow crab megalops 11 27.08 Diogenidae (megalops) left

- handed hermit crabs 8 22.04 Porcellanidae unid. (megalops) porcelain crab megalops 8 21.85 Loligo opalescens market squid 9 20.69 Brachyura unid. (megalops) unidentified crab megalops 8 19.86 Pachycheles pubescens (megalops) pubescent porcelain crab 6 15.80 Lophopanopeus spp. (megalops) black

- clawed crab megalops 4 8.67 Cancer gracilis (megalops) slender crab megalops 4 8.35 Petrolisthes cinctipes (megalops) flat porcelain crab megalops 1 2.63 Petrolisthes eriomerus (megalops) flattop crab megalops 1 2.63 Total Target Invertebrates: 1,397

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 23 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA09 Start Date: 5/1/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 117 411.67 Gobiidae unid. gobies 77 259.33 Sciaenidae unid. croakers 39 141.83 larval fish

- damag ed unidentified larval fishes 7 25.93 Hypsoblennius spp . combtooth blennies 6 21.90 Engraulis mordax northern anchovy 5 17.34 Lepidogobius lepidus bay goby 4 14.49 Icelinus quadriseriatus yellowchin sculpin 4 12.40 Paralichthys californicus California halibut 3 11.12 larval fish fragment unidentified larval fishes 2 7.16 Pleuronichthys guttulatus diamond turbot 1 3.79 Paralichthyidae unid. sand flounders 1 3.57 larvae, unidentified yolksac unidentified yolksac larvae 1 3.37 Chitonotus / Icelinus s culpins 1 3.34 Citharichthys stigmaeus speckled sanddab 1 3.34 Clupeidae unid. herrings 1 3.34 Atherinopsidae unid. silversides 1 2.64 Total Fishes: 271 Eggs fish eggs unid. unidentified fish eggs 1,297 4,334.55 Paralichthyidae unid. (eggs) sand fl ounder eggs 611 2,063.10 Sciaenidae unid. (eggs) croaker eggs 420 1,425.92 Citharichthys spp. (eggs) sanddab eggs 115 409.21 Pleuronichthys spp. (eggs) turbot eggs 42 134.90 Engraulidae unid. (eggs) anchovy eggs 16 53.91 Total Eggs: 2,501 Target Inv ertebrates Pinnixa spp. (megalops) pea crabs megalops 46 133.21 Pugettia spp. (megalops) kelp crabs megalops 20 65.31 Majidae unid. (megalops) spider crab megalops 5 17.41 Paguridae unid. (megalops) hermit crab megalops 3 9.76 unidentified crab (megal ops) unidentified crab megalops 1 3.37 Total Target Invertebrates: 75

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 24 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA10 Start Date: 5/15/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 130 429.83 Genyonemus lineatus white croaker 28 89.20 Lepidogobius lepidus bay goby 19 62.26 Engraulis mordax northern anchovy 16 54.36 Acanthogobius flavimanus yellowfin goby 15 50.50 Hypsoblennius spp . combtooth blennies 11 36.83 Icelinus spp . sculpins 9 30.03 larval fish

- damaged unidentified larval fishes 4 14.53 Gobiesox spp . clingfishes 3 10.13 larval fish fragment unidentified larval fishes 1 3.49 Gillichthys mirabilis longjaw mudsucker 1 3.28 C ottidae unid. sculpins 1 3.10 Sciaenidae unid. croakers 1 3.10 Total Fishes: 239 Eggs fish eggs unid. unidentified fish eggs 269 903.97 Sciaen. / Paralich. / Labridae (eggs) fish eggs 29 95.32 Engraulidae unid. (eggs) anchovy eggs 8 25.98 Pleuronichthys spp. (eggs) turbot eggs 2 6.76 Total Eggs: 308 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 253 832.72 Majidae unid. (megalops) spider crab megalops 119 400.71 unidentified crab (megalops) unidentified crab megalops 15 49.1 4 Pinnixa spp. (megalops) pea crabs megalops 12 38.97 Hemigrapsus spp. (megalops) shore crab megalops 3 10.32 Grapsidae unid. (megalops) shore crab megalops 3 9.10 Grapsidae / Canceridae (megalops) unidentified crab megalops 1 3.59 Paguridae unid. (me galops) hermit crab megalops 1 3.59 Hemigrapsus oregonensis (megs.) yellow shore crab megalops 1 3.49 Pinnotheridae (megalops) pea crab megalops 1 2.91 Total Target Invertebrates: 409

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 25 Harbor Generating Station Entrainment Study - Fish and Inv ertebrate Abundances Survey: HGSEA10 Start Date: 5/15/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 276 564.33 Hypsoblennius spp . combtooth blennies 224 434.26 Genyonemus lineatus white c roaker 201 397.62 Lepidogobius lepidus bay goby 49 99.19 Engraulis mordax northern anchovy 31 64.30 Icelinus spp . sculpins 26 52.38 Gobiesox spp . clingfishes 10 19.79 larval fish fragment unidentified larval fishes 6 11.54 Gillichthys mirabilis longj aw mudsucker 4 7.93 Sebastes spp . rockfishes 2 4.15 Atherinopsis californiensis jacksmelt 2 3.92 unidentified fish, damaged unidentified damaged fish 2 3.83 Bathymasteridae unid. ronquils 2 3.76 larvae, unidentified yolksac unidentified yolksac larvae 1 2.16 Ruscarius creaseri roughcheek sculpin 1 2.16 Typhlogobius californiensis blind goby 1 2.13 Paralichthys californicus California halibut 1 2.09 Rhinogobiops nicholsi blackeye goby 1 2.08 Seriphus politus queenfish 1 2.02 Chitonotus pugetensis roughback sculpin 1 1.82 Sciaenidae unid. croakers 1 1.70 Total Fishes: 843 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 526 1,059.29 unidentified crab (megalops) unidentified crab megalops 153 311.46 Pinnixa spp. (megalops) pea crabs megalops 56 116.60 Grapsidae unid. (megalops) shore crab megalops 12 24.15 P aguridae unid. (megalops) hermit crab megalops 11 22.49 Cancer antennarius (megalops) brown rock crab megalops 9 18.83 Majidae unid. (megalops) spider crab megalops 3 5. 53 Cancer anthonyi (megalops) yellow crab megalops 2 4.17 Lophopanopeus spp. (megalops) black

- clawed crab megalops 1 2.23 Pinnotheres spp. (megalops) pea crab megalops 1 1.90 Total Target Invertebrates: 774

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 26 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA10 Start Date: 5/15/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 129 633.22 Gobiidae unid. gobies 47 234.23 Genyonemus lineatus white croaker 33 180.52 Engraulis mordax northern anchovy 18 82.19 Rhinogobiops nicholsi blackeye goby 3 14.73 unidentified fish, damaged unidentified damaged fish 2 11.93 Gibbonsia spp . clinid kelpfishes 2 11.39 Lepidogobius lepidus bay gob y 2 10.38 Icelinus spp . sculpins 2 8.93 Orthonopias triacis snubnose sculpin 1 6.24 Gillichthys mirabilis longjaw mudsucker 1 4.68 Paralichthys californicus California halibut 1 4.68 Seriphus politus queenfish 1 4.68 Atherinopsidae unid. silversides 1 4.25 Cottidae unid. sculpins 1 4.25 larval fish fragment unidentified larval fishes 1 4.25 Ruscarius creaseri roughcheek sculpin 1 4.25 Total Fishes: 246 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 208 1,052.34 Pinnixa spp. (megalops) pea crabs megalops 91 402.23 unidentified crab (megalops) unidentified crab megalops 58 283.79 Grapsidae unid. (megalops) shore crab megalops 18 84.36 Paguridae unid. (megalops) hermit crab megalops 7 31.46 Cancer anthonyi (megalops) yellow crab megalops 5 23.98 Cancer antennarius (megalops) brown rock crab megalops 3 14.04 Total Target Invertebrates: 390

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 27 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA10 Start Date: 5/15/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 205 553.83 Genyonemus lineatus white croaker 83 185.46 Engraulis mordax northern anchovy 57 155.26 Gobiidae unid. gobies 55 140.06 Leuresthes tenuis California grunion 30 110.80 Engraulidae unid. anchovies 22 68.21 Gobiesox spp . clingfishes 13 34.65 Rhinogobiops nicholsi blackeye goby 10 24.95 larval fish fragment unidentified larval fishes 9 24.39 Typhlogobius californiensis blind goby 9 19.54 Lepidogobius lepidus bay goby 7 16.30 Ruscarius creaseri roughcheek sculpin 2 6.70 Icelinus spp . sculpins 2 3.87 Chitonotus pugetensis roughback sculpin 1 3.83 Clinocottus analis wooly sculpin 1 3.83 Citharichthys stigmaeus speckled sanddab 1 3.69 Cottidae unid. sculpins 1 3.69 Bathymasteridae unid. ronquils 1 2.39 Atherinopsidae unid. silversides 1 2.33 Atherinopsis californiensis jacksmelt 1 2.15 Labrisomidae unid. labrisomid blennies 1 1.71 Paralichthys californicus California halibu t 1 1.71 unidentified fish, damaged unidentified damaged fish 1 1.71 Total Fishes: 514 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 239 582.66 unidentified crab (megalops ) unidentified crab megalops 69 180.96 Pinnixa spp. (megal ops) pea crabs megalops 25 58.33 Grapsidae unid. (megalops) shore crab megalops 10 25.03 Paguridae unid. (megalops) hermit crab megalops 5 10.51 Pinnotheres spp. (megalops) pea crab megalops 4 9.39 Cancer anthonyi (megalops) yellow crab megalops 3 7.69 Panulirus interruptus (phyllosome) California spiny lobster 3 6.87 Cancer antennarius (megalops) brown rock crab megalops 2 4.49 Lophopanopeus spp. (megalops) black

- clawed crab megalops 2 4.31 Cancer spp. (megalops) cancer crabs megalops 1 2.39 Tota l Target Invertebrates: 363

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 28 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA11 Start Date: 5/30/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobie s 262 767.36 Hypsoblennius spp . combtooth blennies 25 72.48 Sciaenidae unid. croakers 17 51.54 Engraulis mordax northern anchovy 16 47.63 Lepidogobius lepidus bay goby 16 45.27 Genyonemus lineatus white croaker 9 30.41 Engraulidae unid. anchovies 9 2 6.92 Paralichthys californicus California halibut 5 15.63 Gillichthys mirabilis longjaw mudsucker 5 15.34 Seriphus politus queenfish 3 10.71 larval fish fragment unidentified larval fishes 3 10.30 larval/post

- larval fish unid. larval fishes 3 9.87 Ac anthogobius flavimanus yellowfin goby 3 7.65 larval fish

- damaged unidentified larval fishes 3 7.38 Pleuronichthys spp . turbots 2 6.73 Gibbonsia spp . clinid kelpfishes 2 5.29 Oxyjulis californica senorita 1 3.40 Rhinogobiops nicholsi blackeye goby 1 3.32 Semicossyphus pulcher California sheephead 1 2.88 Total Fishes: 386 Eggs fish eggs unid. unidentified fish eggs 293 920.33 Sciaen. / Paralich. / Labridae (eggs) fish eggs 107 320.61 Engraulidae unid. (eggs) anchovy eggs 42 130.46 Pleuronichthy s spp. (eggs) turbot eggs 4 11.90 Total Eggs: 446 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 76 229.36 Majidae unid. (megalops) spider crab megalops 34 104.14 unidentified crab (megalops) unidentified crab megalops 23 68.09 Pinnixa spp. (megalops) pea crabs megalops 18 45.38 Paguridae unid. (megalops) hermit crab megalops 2 6.05 Lophopanopeus spp. (megalops) black

- clawed crab megalops 1 2.41 Total Target Invertebrates: 154

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 29 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA12 Start Date: 6/12/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 360 1,198.79 Hypsoblennius spp . combtooth blennies 50 169.93 Sciaenidae unid. croakers 35 113.72 Lepidogobius lepidus bay goby 23 77.56 Paralabrax spp . sand bass 16 51.03 Cheilotrema saturnum black croaker 12 34.56 Engraulidae unid. anchovies 11 33.37 larvae, unidentified yolksac unidentified yolksac larvae 9 29.86 Semicossyp hus pulcher California sheephead 7 23.50 larval fish

- damaged unidentified larval fishes 7 22.85 Gobiesox spp . clingfishes 5 16.53 Seriphus politus queenfish 5 15.28 Genyonemus lineatus white croaker 5 14.80 Engraulis mordax northern anchovy 4 13.52 larval fish fragment unidentified larval fishes 4 12.20 Oxyjulis californica senorita 2 6.24 Paralichthys californicus California halibut 1 4.05 Gillichthys mirabilis longjaw mudsucker 1 3.60 Pleuronectidae unid. righteye flounders 1 3.52 Citharichthys sordidus Pacific sanddab 1 2.72 Pleuronichthys spp . turbots 1 2.72 Rhinogobiops nicholsi blackeye goby 1 2.72 Total Fishes: 561 Eggs fish eggs unid. unidentified fish eggs 221 722.29 Sciaen. / Paralich. / Labridae (eggs) fish eggs 209 685.25 Engraulidae unid. (eggs) anchovy eggs 19 61.27 Total Eggs: 449 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 37 129.28 Majidae unid. (megalops) spider crab megalops 19 65.31 unidentified crab (megalops) unidentified crab megalops 12 36.81 Pinnixa spp. (megalops) pea crabs megalops 5 13.07 Lophopanopeus spp. (megalops) black

- clawed crab megalops 1 3.31 Total Target Invertebrates: 74

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 30 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSE A12 Start Date: 6/12/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 701 1,343.54 Gobiidae unid. gobies 214 445.95 Sciaenidae unid. croakers 75 148.62 Lepidogobius lepidus b ay goby 53 103.37 Seriphus politus queenfish 52 100.83 larvae, unidentified yolksac unidentified yolksac larvae 30 61.91 Engraulis mordax northern anchovy 29 59.87 Cheilotrema saturnum black croaker 21 40.49 Engraulidae unid. anchovies 21 39.56 larval fish fragment unidentified larval fishes 17 35.63 larval fish

- damaged unidentified larval fishes 12 23.62 Semicossyphus pulcher California sheephead 11 22.37 Paralichthys californicus California halibut 11 21.07 Genyonemus lineatus white croaker 8 16.13 Gobiesox spp . clingfishes 6 11.22 Paralabrax spp . sand bass 5 10.39 Sphyraena argentea Pacific barracuda 5 9.17 Roncador stearnsi spotfin croaker 4 7.53 Icelinus spp . sculpins 3 6.56 Hypsypops rubicundus garibaldi 3 5.85 Oxyjulis californica s enorita 3 5.76 Labrisomidae unid. labrisomid blennies 3 4.83 Ruscarius creaseri roughcheek sculpin 2 4.24 Peprilus simillimus Pacific butterfish 2 3.52 Umbrina roncador yellowfin croaker 2 3.52 Artedius lateralis smoothhead sculpin 1 2.36 Clinocottus analis wooly sculpin 1 2.36 Pleuronectidae unid. righteye flounders 1 2.30 Pleuronectiformes unid. flatfishes 1 2.20 Citharichthys spp . sanddabs 1 1.96 Cottidae unid. sculpins 1 1.92 larval/post

- larval fish unid. larval fishes 1 1.92 Pleuronichthys guttulatus diamond turbot 1 1.92 Gibbonsia spp . clinid kelpfishes 1 1.76

, Atherinopsidae unid. silversides 1 1.61 Labridae unid. wrasses 1 1.61 Leuresthes tenuis California grunion 1 1.61 Total Fishes: 1,305 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 85 173.87 Majidae unid. (megalops) spider crab megalops 30 60.29 Pinnixa spp. (megalops) pea crabs megalops 15 29.64 Diogenidae (megalops) left

- handed hermit crabs 3 5.94 (continued)

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 31 Harbor Generating Station Entrainmen t Study - Fish and Invertebrate Abundances Survey: HGSEA12 Start Date: 6/12/2006 Stations: H1 - H3 (continued) Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Target Invertebrates (continued) Paguridae unid. (megalops) hermit crab megalops 3 5 .94 Grapsidae unid. (megalops) shore crab megalops 3 5.67 Lophopanopeus spp. (megalops) black

- clawed crab megalops 2 4.59 Panulirus interruptus (phyllosome) California spiny lobster 1 2.04 Cancer anthonyi (megalops) yellow crab megalops 1 1.80 Total Target Invertebrates: 143

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 32 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA12 Start Date: 6/12/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 82 497.47 Gobiidae unid. gobies 53 328.31 Engraulis mordax northern anchovy 17 100.47 Sciaenidae unid. croakers 15 94.32 Seriphus politus queenfish 10 62.21 Engraulidae unid. anchovies 8 49.17 larval fish

- damaged unidentified larval fishes 7 43.13 larvae, unidentified yolksac unidentified yolksac larvae 6 36.47 Oxyjulis californica senorita 4 24.24 larval fish fragment unidentified larval fishes 4 23.50 Cheilotrema saturnum black croaker 3 18.23 Labrisomidae unid. labrisomid blennies 2 13.01 Clinocottus analis wooly sculpin 2 12.97 Paralichthys californicus California halibut 2 12.41 Paralabrax spp . sand bass 2 11.75 Syngnathus spp . pipefishes 2 11.75 Paralichthyidae unid. sand flounders 1 6.52 Artedius lateralis smoothh ead sculpin 1 6.49 Ruscarius creaseri roughcheek sculpin 1 6.49 Semicossyphus pulcher California sheephead 1 6.49 Hypsypops rubicundus garibaldi 1 5.92 Oligocottus / Clinocottus sculpins 1 5.92 Rhinogobiops nicholsi blackeye goby 1 5.92 Zaniolepis sp p . combfishes 1 5.92 Gibbonsia spp . clinid kelpfishes 1 5.87 Lepidogobius lepidus bay goby 1 5.87 Pleuronichthys verticalis hornyhead turbot 1 5.87 Sphyraena argentea Pacific barracuda 1 5.87 Total Fishes: 231 Target Invertebrates Pinnixa spp. (meg alops) pea crabs megalops 14 82.85 Pugettia spp. (megalops) kelp crabs megalops 11 69.67 unidentified crab (megalops) unidentified crab megalops 9 56.78 Majidae unid. (megalops) spider crab megalops 6 37.13 Grapsidae unid. (megalops) shore crab megalop s 3 18.89 Lophopanopeus spp. (megalops) black

- clawed crab megalops 2 12.97 Diogenidae (megalops) left

- handed hermit crabs 1 6.52 Pinnotheres spp. (megalops) pea crab megalops 1 6.49 Cancer anthonyi (megalops) yellow crab megalops 1 5.92 Total Target Invertebrates: 48

### Harbor Generating Statio n

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C1 - 33 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA12 Start Date: 6/12/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 273 789.40 Sciaenidae unid. croakers 86 251.74 Seriphus politus queenfish 89 239.33 Engraulis mordax northern anchovy 56 155.88 Gobiidae unid. gobies 41 129.67 Engraulidae unid. anchovies 34 108.12 larvae, unidentified yolksac unidentified yolksac larvae 35 94.08 Gobiesox spp . clingfishes 20 67.99 larval fish

- damaged unidentified larval fishes 22 59.30 Genyonemus lineatus white croaker 25 56.09 larval fish fragment unidentified larval fishes 19 48.15 Paralichthys californicus California halibut 17 47.16 Oxyjulis californica senorita 13 35.96 Rhinogobiops nicholsi blackeye goby 11 35.61 Semicossyphus pulcher California sheephead 11 35.53 Sphyraena argentea Pacific barracuda 11 29.94 Paralabrax spp . sand bass 11 29.39 Labrisomidae unid. labrisomid blennies 7 21.21 Pleuronichthys verticalis hornyhead turbot 7 18.44 Hypsypops rubicundus garibaldi 6 17.95 Pleuronichthys ritteri spotted turbot 4 13.75 Lepidogobius lepidus bay goby 4 11.29 Cheilotrema saturnum black croaker 3 8.23 Roncador stearnsi spotfin croaker 3 7.61 Atherinopsidae unid. silversides 3 6.73 Umbrina roncador yellowfin croaker 2 6.46 Ruscarius creaseri roughcheek sculpin 2 5.88 Pleuronectidae unid. righteye flounders 2 5.64 Zaniolepis frenata shortspine combf ish 2 4.94 Menticirrhus undulatus California corbina 1 4.01 Syngnathus spp . pipefishes 1 4.01 Citharichthys sordidus Pacific sanddab 1 3.86 larval/post

- larval fish unid. larval fishes 1 3.86 Zaniolepis spp . combfishes 1 3.52 Hypsoblennius jenkinsi mu ssel blenny 1 2.94 Citharichthys stigmaeus speckled sanddab 1 2.24 Halichoeres semicinctus rock wrasse 1 2.24 Leuresthes tenuis California grunion 1 2.24 Pleuronectiformes unid. flatfishes 1 2.24 Pomacentridae unid. damselfishes 1 2.24 Total Fishes: 830 (continued)

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C1 - 34 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA12 Start Date: 6/12/2006 Stations: H5 - H6 (continued) Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Target Invertebrates Pu gettia spp. (megalops) kelp crabs megalops 70 202.42 Pinnixa spp. (megalops) pea crabs megalops 29 80.07 Majidae unid. (megalops) spider crab megalops 20 54.21 Lophopanopeus spp. (megalops) black

- clawed crab megalops 5 15.66 Diogenidae (megalops) left

- handed hermit crabs 5 14.40 unidentified crab (megalops) unidentified crab megalops 4 13.48 Paguridae unid. (megalops) hermit crab megalops 3 11.73 Petrolisthes spp. (megalops) porcelain crab megalops 3 8.35 Grapsidae unid. (megalops) shore crab megal ops 3 8.23 Pinnotheres spp. (megalops) pea crab megalops 1 3.86 Porcellanidae unid. (megalops) porcelain crab megalops 1 3.86 Cancer anthonyi (megalops) yellow crab megalops 1 2.54 Panulirus interruptus (phyllosome) California spiny lobster 1 2.24 To tal Target Invertebrates: 146

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C1 - 35 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA13 Start Date: 6/26/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 157 524.47 Hypsoblennius spp . combtooth blennies 27 91.31 Lepidogobius lepidus bay goby 13 43.87 Genyonemus lineatus white croaker 4 13.24 Engraulidae unid. anchovies 2 6.73 Artedius harringtoni scalyhead sculpin 1 3.13 larval fish fragment unidentified larval fishes 1 3.13 Gibbonsia spp . clinid kelpfishes 1 2.91 Total Fishes: 206 Eggs fish eggs unid. unidentified fish eggs 79 263.49 Sciaen. / Paralich. / Labridae (eggs) fish eggs 34 113.22 Pleuronichthys spp. (eggs) turbot eggs 3 10.69 Engraulidae unid. (eggs) anchovy eggs 1 3.13 Total Eggs: 117 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 18 60.91 Pinnixa spp. (megalops) pea crabs megalops 15 51.95 Majidae unid. (megalops) spider crab megalops 11 37.53 unide ntified crab (megalops) unidentified crab megalops 5 17.45 Lophopanopeus spp. (megalops) black

- clawed crab megalops 1 3.60 Total Target Invertebrates: 50

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C1 - 36 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: H GSEA14 Start Date: 7/10/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 63 191.84 Hypsoblennius spp . combtooth blennies 29 85.70 Lepidogobius lepidus bay goby 22 65.70 larval fish fragment uni dentified larval fishes 3 9.14 Gobiesox spp . clingfishes 3 9.04 larval/post

- larval fish unid. larval fishes 1 3.37 Seriphus politus queenfish 1 2.89 Total Fishes: 122 Eggs fish eggs unid. unidentified fish eggs 27 168.68 Total Eggs: 27 Target Inv ertebrates Pugettia spp. (megalops) kelp crabs megalops 27 80.41 unidentified crab (megalops) unidentified crab megalops 10 30.00 Pinnixa spp. (megalops) pea crabs megalops 9 27.93 Panulirus interruptus (phyllosome) California spiny lobster 4 12.27 B rachyura unid. (megalops) unidentified crab megalops 3 9.47 Pinnotheres spp. (megalops) pea crab megalops 2 6.27 Majidae unid. (megalops) spider crab megalops 2 5.87 Grapsidae unid. (megalops) shore crab megalops 1 3.37 Total Target Invertebrates: 58

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C1 - 37 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA14 Start Date: 7/10/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 173 3 13.17 Gobiidae unid. gobies 112 206.53 Lepidogobius lepidus bay goby 27 48.53 Engraulis mordax northern anchovy 8 14.33 larval fish fragment unidentified larval fishes 3 5.23 Sciaenidae unid. croakers 2 3.55 Syngnathus spp . pipefishes 1 1.87 Icelinu s spp . sculpins 1 1.77 Gobiesox spp . clingfishes 1 1.72 Total Fishes: 328 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 49 86.60 Panulirus interruptus (phyllosome) California spiny lobster 20 35.99 Pinnixa spp. (megalops) pea cr abs megalops 17 30.32 unidentified crab (megalops) unidentified crab megalops 12 20.35 Brachyura unid. (megalops) unidentified crab megalops 2 3.31 Grapsidae unid. (megalops) shore crab megalops 1 1.77 Cancer anthonyi (megalops) yellow crab megalops 1 1.68 Majidae unid. (megalops) spider crab megalops 1 1.65 Pinnotheres spp. (megalops) pea crab megalops 1 1.65 Total Target Invertebrates: 104

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C1 - 38 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA14 Sta rt Date: 7/10/2006 Stations: H4 Mean Sample Count: 4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 31 182.44 Gobiidae unid. gobies 18 100.32 Gobiesox spp . clingfishes 2 11.76 Lepidogobius lepidus bay goby 2 9.57 Total Fishes: 53 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 7 39.96 Pinnixa spp. (megalops) pea crabs megalops 6 39.79 Panulirus interruptus (phyllosome) California spiny lobster 4 24.68 unidentified crab (megalops) unidentified crab megalops 2 10.66 Brachyura unid. (megalops) unidentified crab megalops 1 6.63 Grapsidae unid. (megalops) shore crab megalops 1 6.63 Majidae unid. (megalops) spider crab megalops 1 5.86 Pinnotheres spp. (megalops) pea crab megalops 1 4.78 Total Target Invertebrates: 23

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C1 - 39 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA14 Start Date: 7/10/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fis hes Hypsoblennius spp . combtooth blennies 130 351.98 Gobiidae unid. gobies 24 68.70 Lepidogobius lepidus bay goby 20 47.19 Engraulis mordax northern anchovy 11 24.28 Gobiesox spp . clingfishes 5 12.15 Labrisomidae unid. labrisomid blennies 3 9.61 larval fish

- damaged unidentified larval fishes 3 9.50 Zaniolepis frenata shortspine combfish 3 7.68 Icelinus spp . sculpins 2 5.24 Sciaenidae unid. croakers 2 4.18 Pomacentridae unid. damselfishes 1 3.50 larval fish fragment unidentified larval fishes 1 3.26 Pleuronichthys spp . turbots 1 2.50 Typhlogobius californiensis blind goby 1 2.50 Cheilotrema saturnum black croaker 1 2.09 Gibbonsia spp . clinid kelpfishes 1 2.09 Total Fishes: 209 Target Invertebrates Panulirus interruptus (phyllosome) Calif ornia spiny lobster 234 553.29 Pugettia spp. (megalops) kelp crabs megalops 24 69.29 Pinnixa spp. (megalops) pea crabs megalops 16 37.31 unidentified crab (megalops) unidentified crab megalops 6 17.84 Majidae unid. (megalops) spider crab megalops 4 12 .39 Lophopanopeus spp. (megalops) black

- clawed crab megalops 5 12.03 Cancer anthonyi (megalops) yellow crab megalops 2 4.59 Grapsidae unid. (megalops) shore crab megalops 1 2.50 Diogenidae (megalops) left

- handed hermit crabs 1 2.09 Porcellanidae unid . (megalops) porcelain crab megalops 1 2.09 Total Target Invertebrates: 294

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C1 - 40 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA15 Start Date: 7/24/2006 Stations: E1 Mean Concentration Taxon Common Name C ount (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 33 107.50 Gobiidae unid. gobies 29 100.14 Lepidogobius lepidus bay goby 10 32.66 Engraulidae unid. anchovies 1 3.63 larvae, unidentified yolksac unidentified yolksac larvae 1 3.63 Sciaenidae unid. croakers 1 3.63 Syngnathus spp . pipefishes 1 3.63 Rhinogobiops nicholsi blackeye goby 1 3.38 Cheilotrema saturnum black croaker 1 3.03 larval fish

- damaged unidentified larval fishes 1 3.03 Total Fishes: 79 Eggs fish eggs unid. unidentified fish eggs 22 145.68 Sciaen. / Paralich. / Labridae (eggs) fish eggs 7 44.47 Total Eggs: 29 Target Invertebrates Panulirus interruptus (phyllosome) California spiny lobster 7 25.51 Pinnixa spp. (megalops) pea crabs megalops 3 10.29 Majidae unid. (megalops) spider crab megalops 1 3.27 Total Target Invertebrates: 11

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C1 - 41 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA16 Start Date: 8/7/2006 Stations: E1 Mean Concentration Taxon Common Name C ount (#/1,000m 3 ) Fishes Gobiidae unid. gobies 90 261.40 Lepidogobius lepidus bay goby 18 51.80 Hypsoblennius spp . combtooth blennies 8 25.07 Rhinogobiops nicholsi blackeye goby 2 5.43 Acanthogobius flavimanus yellowfin goby 1 3.35 larvae, unidentified yolksac unidentified yolksac larvae 1 3.13 Lythrypnus spp . gobies 1 2.01 Sciaenidae unid. croakers 1 2.01 Total Fishes: 122 Eggs fish eggs unid. unidentified fish eggs 207 1,024.20 Atherinopsidae unid. (eggs) silverside eggs 1 7.11 Total Eggs : 208 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 16 50.00 Majidae unid. (megalops) spider crab megalops 9 28.38 Panulirus interruptus (phyllosome) California spiny lobster 9 25.27 Pinnixa spp. (megalops) pea crabs megalops 7 1 6.74 Total Target Invertebrates: 41

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C1 - 42 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA16 Start Date: 8/7/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsob lennius spp . combtooth blennies 128 273.94 Gobiidae unid. gobies 114 249.19 Lepidogobius lepidus bay goby 73 148.93 Rhinogobiops nicholsi blackeye goby 5 10.93 larval fish fragment unidentified larval fishes 4 9.04 Engraulis mordax northern anchovy 4 8.67 Lythrypnus spp . gobies 3 7.43 Acanthogobius flavimanus yellowfin goby 3 6.34 Labrisomidae unid. labrisomid blennies 2 4.26 larvae, unidentified yolksac unidentified yolksac larvae 1 2.37 Genyonemus lineatus white croaker 1 2.21 Icelinus spp . scu lpins 1 2.14 Ilypnus gilberti cheekspot goby 1 2.12 Gobiesox spp . clingfishes 1 2.11 Pleuronichthys spp . turbots 1 1.95 larval/post

- larval fish unid. larval fishes 1 1.80 Total Fishes: 343 Target Invertebrates Pugettia spp. (megalops) kelp crabs me galops 43 92.67 Panulirus interruptus (phyllosome) California spiny lobster 33 74.73 Majidae unid. (megalops) spider crab megalops 21 45.66 Pinnixa spp. (megalops) pea crabs megalops 11 24.84 Diogenidae (megalops) left

- handed hermit crabs 1 2.12 Total Target Invertebrates: 109

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C1 - 43 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA16 Start Date: 8/7/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. go bies 41 256.97 Hypsoblennius spp . combtooth blennies 35 219.13 Lepidogobius lepidus bay goby 7 40.99 Rhinogobiops nicholsi blackeye goby 2 13.29 Pleuronichthys spp . turbots 2 12.67 Gobiesox spp . clingfishes 1 6.78 Labrisomidae unid. labrisomid blenni es 1 6.65 Lythrypnus spp . gobies 1 6.65 Sciaenidae unid. croakers 1 6.65 Genyonemus lineatus white croaker 1 6.02 Icelinus spp . sculpins 1 6.02 Syngnathus leptorhynchus bay pipefish 1 6.02 Gobiesocidae unid. clingfishes 1 5.45 Total Fishes: 95 Tar get Invertebrates Panulirus interruptus (phyllosome) California spiny lobster 11 66.40 Majidae unid. (megalops) spider crab megalops 10 58.58 Pugettia spp. (megalops) kelp crabs megalops 6 37.50 Pinnixa spp. (megalops) pea crabs megalops 4 23.51 Total Target Invertebrates: 31

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C1 - 44 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA16 Start Date: 8/7/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius sp p . combtooth blennies 130 387.88 Gobiidae unid. gobies 48 136.03 Lepidogobius lepidus bay goby 9 25.30 Gobiesox spp . clingfishes 5 13.67 Syngnathus spp . pipefishes 3 10.80 Hypsypops rubicundus garibaldi 3 8.81 Seriphus politus queenfish 2 6.00 Lythr ypnus spp . gobies 2 5.56 Paralichthyidae unid. sand flounders 1 3.41 Engraulidae unid. anchovies 1 3.00 Pleuronichthys ritteri spotted turbot 1 3.00 Gibbonsia spp . clinid kelpfishes 1 2.89 Citharichthys stigmaeus speckled sanddab 1 2.82 Rhinogobiops nicholsi blackeye goby 1 2.82 Acanthogobius flavimanus yellowfin goby 1 2.70 Genyonemus lineatus white croaker 1 2.70 larval fish fragment unidentified larval fishes 1 2.70 Paralichthys californicus California halibut 1 2.70 Sciaenidae unid. croakers 1 2.70 Hypsoblennius jenkinsi mussel blenny 1 2.57 Labrisomidae unid. labrisomid blennies 1 2.52 Pleuronectidae unid. righteye flounders 1 2.52 Total Fishes: 216 Target Invertebrates Panulirus interruptus (phyllosome) California spiny lobster 30 81 .35 Pugettia spp. (megalops) kelp crabs megalops 29 79.69 Majidae unid. (megalops) spider crab megalops 17 49.27 Pinnixa spp. (megalops) pea crabs megalops 11 31.22 Diogenidae (megalops) left

- handed hermit crabs 2 5.59 Total Target Invertebrates: 89

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C1 - 45 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA17 Start Date: 8/21/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 40 128.16 Lepidogobius lepidus bay goby 23 73.41 Hypsoblennius spp . combtooth blennies 11 35.09 Labrisomidae unid. labrisomid blennies 1 3.33 Rhinogobiops nicholsi blackeye goby 1 3.33 Gobiesox spp . clingfishes 1 3.05 Sciaenidae unid. croakers 1 3.05 Lythrypnus zebra zebr a goby 1 2.92 Total Fishes: 79 Eggs fish eggs unid. unidentified fish eggs 22 124.28 Paralichthyidae unid. (eggs) sand flounder eggs 1 6.01 Total Eggs: 23 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 3 9.71 Pinnixa spp. (megalops) pea crabs megalops 2 6.61 Total Target Invertebrates: 5 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA18 Start Date: 9/5/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 36 122.67 Hypsoblennius spp . combtooth blennies 12 42.13 Gobiesox spp . clingfishes 1 3.79 Pleuronichthys guttulatus diamond turbot 1 3.51 Pleuronichthys spp . turbots 1 3.51 Total Fishes: 51 Eggs fish eggs unid. unidentified fish eggs 39 267.10 Sciaen. / Paralich. / Labridae (eggs) fish eggs 2 14.71 Paralichthyidae unid. (eggs) sand flounder eggs 1 7.56 Total Eggs: 42 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 11 40.11 Pinnixa spp. (meg alops) pea crabs megalops 7 22.12 Majidae unid. (megalops) spider crab megalops 6 21.32 Total Target Invertebrates: 24

### Harbor Generating Statio n

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C1 - 46 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA18 Start Date: 9/5/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 133 282.36 Hypsoblennius spp . combtooth blennies 123 253.69 Lepidogobius lepidus bay goby 50 104.05 Seriphus politus queenfish 22 45.56 Engraulidae unid. anc hovies 5 10.27 Pleuronichthys ritteri spotted turbot 3 5.99 larval fish

- damaged unidentified larval fishes 1 2.34 Roncador stearnsi spotfin croaker 1 2.34 Artedius lateralis smoothhead sculpin 1 2.18 Syngnathus spp . pipefishes 1 2.13 larval fish fr agment unidentified larval fishes 1 2.12 Genyonemus lineatus white croaker 1 2.00 Gobiesox spp . clingfishes 1 1.90 Total Fishes: 343 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 60 120.38 Majidae unid. (megalops) spider crab meg alops 33 69.79 Pinnixa spp. (megalops) pea crabs megalops 18 37.45 Diogenidae (megalops) left

- handed hermit crabs 1 2.13 Total Target Invertebrates: 112

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C1 - 47 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA18 Start Date: 9/5/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Hypsoblennius spp . combtooth blennies 20 153.81 Gobiidae unid. gobies 4 31.84 Genyonemus lineatus white croaker 2 19.95 Lepidogobius lepidus b ay goby 2 19.95 Pleuronichthys ritteri spotted turbot 2 11.94 Paralichthyidae unid. sand flounders 1 6.40 Pleuronichthys guttulatus diamond turbot 1 6.40 Pleuronichthys spp . turbots 1 6.40 Citharichthys sordidus Pacific sanddab 1 5.92 Paralichthys ca lifornicus California halibut 1 5.92 Syngnathus spp . pipefishes 1 5.92 Total Fishes: 36 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 5 39.16 Pinnixa spp. (megalops) pea crabs megalops 2 27.96 Majidae unid. (megalops) spider crab megalops 1 13.98 Total Target Invertebrates: 8

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C1 - 48 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA18 Start Date: 9/5/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fi shes Gobiidae unid. gobies 63 218.53 Hypsoblennius spp . combtooth blennies 52 178.26 Seriphus politus queenfish 30 102.49 Pleuronichthys ritteri spotted turbot 16 44.39 Lepidogobius lepidus bay goby 11 37.78 Genyonemus lineatus white croaker 7 19.12 Engraulidae unid. anchovies 5 17.26 Sciaenidae unid. croakers 2 8.92 Paralichthys californicus California halibut 3 7.99 Gobiesox spp . clingfishes 1 6.31 Pleuronichthys spp . turbots 2 5.74 larval fish

- damaged unidentified larval fishes 2 5.65 Chei lotrema saturnum black croaker 1 3.48 Menticirrhus undulatus California corbina 1 2.69 Total Fishes: 196 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 49 156.86 Pinnixa spp. (megalops) pea crabs megalops 21 100.94 Majidae unid. ( megalops) spider crab megalops 21 75.30 Panulirus interruptus (phyllosome) California spiny lobster 9 30.35 Petrolisthes spp. (megalops) porcelain crab megalops 3 15.67 Diogenidae (megalops) left

- handed hermit crabs 1 3.05 Grapsidae unid. (megalops) shore crab megalops 1 3.05 Lophopanopeus spp. (megalops) black

- clawed crab megalops 1 2.69 Total Target Invertebrates: 106

### Harbor Generating Statio n

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C1 - 49 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA19 Start Date: 9/18/2006 Stati ons: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 57 209.68 Lepidogobius lepidus bay goby 14 49.88 Hypsoblennius spp . combtooth blennies 8 28.23 larval fish fragment unidentified larval fishes 1 3.86 larval fish

- damaged unidentified larval fishes 1 3.67 Lythrypnus zebra zebra goby 1 3.11 Pleuronichthys guttulatus diamond turbot 1 2.74 Total Fishes: 83 Eggs fish eggs unid. unidentified fish eggs 17 114.89 Paralichthyidae unid. (eggs) sand flounder eggs 2 13.70 Total Eggs: 19 Target Invertebrates Pinnixa spp. (megalops) pea crabs megalops 8 26.74 Pugettia spp. (megalops) kelp crabs megalops 5 17.54 Majidae unid. (megalops) spider crab megalops 2 6.48 Grapsidae unid. (megalops) shore crab megalops 1 3.38 Total Target Invertebrates: 16

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C1 - 50 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA20 Start Date: 10/2/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes G obiidae unid. gobies 275 904.86 Hypsoblennius spp . combtooth blennies 2 6.28 Lepidogobius lepidus bay goby 1 3.76 Roncador stearnsi spotfin croaker 1 3.76 Hypsoblennius gilberti rockpool blenny 1 3.48 larval fish

- damaged unidentified larval fishes 1 2.64 Sciaenidae unid. croakers 1 2.64 Total Fishes: 282 Eggs fish eggs unid. unidentified fish eggs 5 33.62 Citharichthys spp. (eggs) sanddab eggs 1 6.33 Total Eggs: 6 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 34 117.62 Majidae unid. (megalops) spider crab megalops 9 30.39 Pinnixa spp. (megalops) pea crabs megalops 5 15.71 Grapsidae unid. (megalops) shore crab megalops 2 6.16 Brachyura unid. (megalops) unidentified crab megalops 1 3.77 Total Target Invertebrates: 51

### Harbor Generating Statio n

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C1 - 51 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA21 Start Date: 10/16/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 90 312.39 Hypsoblenniu s spp . combtooth blennies 3 9.25 Engraulidae unid. anchovies 2 7.20 Engraulis mordax northern anchovy 2 6.84 Citharichthys stigmaeus speckled sanddab 1 3.48 Gibbonsia spp . clinid kelpfishes 1 3.45 Gobiesox spp . clingfishes 1 3.44 Genyonemus lineatus white croaker 1 3.25 larval fish fragment unidentified larval fishes 1 3.09 Lepidogobius lepidus bay goby 1 3.09 Total Fishes: 103 Eggs fish eggs unid. unidentified fish eggs 12 78.79 Paralichthyidae unid. (eggs) sand flounder eggs 2 13.64 Pleuroni chthys spp. (eggs) turbot eggs 1 5.91 Total Eggs: 15 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 70 232.92 Majidae unid. (megalops) spider crab megalops 15 50.95 Pinnixa spp. (megalops) pea crabs megalops 6 21.90 Total Target I nvertebrates: 91

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IM&E Final Report Appendix C1: Entrainment Data

C1 - 52 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA21 Start Date: 10/16/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 77 182.09 Hypsoblennius spp . combtooth blennies 49 113.02 Genyonemus lineatus white croaker 11 26.06 Lepidogobius lepidus bay goby 8 18.45 Paralichthyidae unid. sand flounders 1 2.33 Seriphus politus queenfish 1 2.28 Sciaenidae unid. croakers 1 2.26 S yngnathus spp . pipefishes 1 2.22 larval fish

- damaged unidentified larval fishes 1 1.83 Total Fishes: 150 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 128 300.75 Majidae unid. (megalops) spider crab megalops 38 89.64 Pinnixa sp p. (megalops) pea crabs megalops 17 41.54 Grapsidae unid. (megalops) shore crab megalops 2 4.80 Lophopanopeus spp. (megalops) black

- clawed crab megalops 1 2.65 Panulirus interruptus (phyllosome) California spiny lobster 1 2.22 Total Target Invertebrat es: 187

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 53 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA21 Start Date: 10/16/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 35 226.92 Geny onemus lineatus white croaker 20 136.02 Hypsoblennius spp . combtooth blennies 10 63.77 larval fish fragment unidentified larval fishes 3 16.87 Pleuronichthys ritteri spotted turbot 2 13.92 Lepidogobius lepidus bay goby 1 7.20 Clinocottus analis wooly sculpin 1 6.72 larvae, unidentified yolksac unidentified yolksac larvae 1 6.72 Engraulidae unid. anchovies 1 5.14 Total Fishes: 74 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 17 112.82 Majidae unid. (megalops) spider crab megal ops 16 105.75 Pinnixa spp. (megalops) pea crabs megalops 10 61.22 Panulirus interruptus (phyllosome) California spiny lobster 2 13.92 Total Target Invertebrates: 45

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 54 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundanc es Survey: HGSEA21 Start Date: 10/16/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 59 182.73 Gobiidae unid. gobies 43 126.79 Hypsoblennius spp . combtooth blennies 35 101.92 l arvae, unidentified yolksac unidentified yolksac larvae 24 75.98 Engraulidae unid. anchovies 17 50.20 Lepidogobius lepidus bay goby 8 22.69 Paralichthys californicus California halibut 5 15.64 Pleuronichthys ritteri spotted turbot 3 8.81 Pleuronichthy s guttulatus diamond turbot 3 8.77 larval fish

- damaged unidentified larval fishes 2 5.89 Paralichthyidae unid. sand flounders 2 5.81 larval fish fragment unidentified larval fishes 2 5.56 Citharichthys stigmaeus speckled sanddab 1 3.66 Pleuronectifo rmes unid. flatfishes 1 3.10 Engraulis mordax northern anchovy 1 3.04 Pleuronichthys verticalis hornyhead turbot 1 3.04 Sciaenidae unid. croakers 1 2.85 Seriphus politus queenfish 1 2.85 unidentified fish, damaged unidentified damaged fish 1 2.75 Total Fishes: 210 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 86 245.67 Majidae unid. (megalops) spider crab megalops 45 129.84 Pinnixa spp. (megalops) pea crabs megalops 11 31.16 Panulirus interruptus (phyllosome) California spiny lobster 2 5.79 Brachyura unid. (megalops) unidentified crab megalops 2 5.50 Porcellanidae unid. (megalops) porcelain crab megalops 1 3.66 Paguridae unid. (megalops) hermit crab megalops 1 2.85 Total Target Invertebrates: 148

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 55 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA22 Start Date: 10/30/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 57 189.98 Genyonemus lineatus white croaker 5 16. 72 Hypsoblennius spp . combtooth blennies 1 3.68 Lepidogobius lepidus bay goby 1 3.51 Engraulidae unid. anchovies 1 3.32 Engraulis mordax northern anchovy 1 2.95 larval fish fragment unidentified larval fishes 1 2.95 Sciaenidae unid. croakers 1 2.95 Total Fishes: 68 Eggs fish eggs unid. unidentified fish eggs 12 81.36 Paralichthyidae unid. (eggs) sand flounder eggs 2 14.56 Engraulidae unid. (eggs) anchovy eggs 1 7.47 Pleuronichthys spp. (eggs) turbot eggs 1 6.74 Total Eggs: 16 Target Inverteb rates Pugettia spp. (megalops) kelp crabs megalops 129 449.67 Majidae unid. (megalops) spider crab megalops 31 105.05 Pinnixa spp. (megalops) pea crabs megalops 5 17.19 Grapsidae unid. (megalops) shore crab megalops 1 3.57 Paguridae unid. (megalops) h ermit crab megalops 1 3.56 Total Target Invertebrates: 167

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 56 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA23 Start Date: 11/13/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000 m 3 ) Fishes Gobiidae unid. gobies 34 113.64 Genyonemus lineatus white croaker 12 40.96 larvae, unidentified yolksac unidentified yolksac larvae 2 6.88 larval fish fragment unidentified larval fishes 2 6.63 Hypsoblennius spp . combtooth blennies 2 6. 26 Sciaenidae unid. croakers 1 3.52 Engraulidae unid. anchovies 1 3.19 Engraulis mordax northern anchovy 1 3.19 Total Fishes: 55 Eggs fish eggs unid. unidentified fish eggs 16 107.58 Paralichthyidae unid. (eggs) sand flounder eggs 4 24.50 Sciaen. / Paralich. / Labridae (eggs) fish eggs 2 13.15 Sciaenidae unid. (eggs) croaker eggs 1 6.98 Pleuronichthys spp. (eggs) turbot eggs 1 6.44 Total Eggs: 24 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 36 118.46 Majidae unid. (megal ops) spider crab megalops 17 55.94 Pinnixa spp. (megalops) pea crabs megalops 3 10.00 Brachyura unid. (megalops) unidentified crab megalops 2 6.95 Total Target Invertebrates: 58

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 57 Harbor Generating Station Entrainment Study - Fish and Invertebra te Abundances Survey: HGSEA23 Start Date: 11/13/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 105 194.57 Genyonemus lineatus white croaker 76 152.40 Hypsoblennius spp . combtooth blennies 17 35.16 Lepidogobius lepidus bay goby 9 16.67 larvae, unidentified yolksac unidentified yolksac larvae 6 12.15 Engraulis mordax northern anchovy 6 11.41 Pleuronichthys guttulatus diamond turbot 5 9.89 Engraulidae unid. anchovies 3 6.57 larval fish f

ragment unidentified larval fishes 3 6.26 Seriphus politus queenfish 2 4.13 Pleuronichthys spp . turbots 1 2.25 Gillichthys mirabilis longjaw mudsucker 1 1.97 Clinocottus analis wooly sculpin 1 1.84 Citharichthys stigmaeus speckled sanddab 1 1.78 Sciaenidae unid

. croakers 1 1.55 Total Fishes: 237 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 111 217.96 Majidae unid. (megalops) spider crab megalops 40 81.15 Pinnixa spp. (megalops) pea crabs megalops 8 15.58 Brachyura unid. (me galops) unidentified crab megalops 3 5.65 Grapsidae unid. (megalops) shore crab megalops 1 1.55 Total Target Invertebrates: 163

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 58 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA23 Start Date: 11/13/2 006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 273 1,625.77 Gobiidae unid. gobies 100 558.53 larvae, unidentified yolksac unidentified yolksac larvae 12 68.50 larval fish fragment unidentified larval fishes 8 49.65 Sciaenidae unid. croakers 6 37.15 Engraulidae unid. anchovies 1 6.24 Hypsoblennius spp . combtooth blennies 1 6.17 larval fish

- damaged unidentified larval fishes 1 6.17 Total Fishes: 402 Target Invertebrates Puge ttia spp. (megalops) kelp crabs megalops 50 289.75 Majidae unid. (megalops) spider crab megalops 29 174.13 Pinnixa spp. (megalops) pea crabs megalops 5 26.76 Grapsidae unid. (megalops) shore crab megalops 2 10.95 Total Target Invertebrates: 86

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 59 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA23 Start Date: 11/13/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 542 1,512.22 l arvae, unidentified yolksac unidentified yolksac larvae 60 171.68 Gobiidae unid. gobies 47 132.68 Engraulis mordax northern anchovy 35 99.31 Pleuronichthys guttulatus diamond turbot 22 63.10 Paralichthys californicus California halibut 17 48.66 Hypsob lennius spp . combtooth blennies 12 33.36 Lepidogobius lepidus bay goby 11 29.25 larval fish fragment unidentified larval fishes 7 19.76 Citharichthys stigmaeus speckled sanddab 5 13.69 Pleuronichthys ritteri spotted turbot 4 11.58 Paralichthyidae unid

. sand flounders 2 5.97 Clinocottus analis wooly sculpin 2 5.60 Sciaenidae unid. croakers 2 5.38 Engraulidae unid. anchovies 1 3.14 Xystreurys liolepis fantail sole 1 3.14 Pleuronichthys verticalis hornyhead turbot 1 2.98 Pleuronichthys spp . turbots 1 2.69 Triphoturus mexicanus Mexican lampfish 1 2.69 Total Fishes: 773 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 70 191.62 Majidae unid. (megalops) spider crab megalops 25 72.95 Pinnixa spp. (megalops) pea crabs megalops 12 3 4.30 Brachyura unid. (megalops) unidentified crab megalops 2 5.26 Cancer anthonyi (megalops) yellow crab megalops 1 3.14 Paguridae unid. (megalops) hermit crab megalops 1 2.63 Total Target Invertebrates: 111

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 60 Harbor Generating Station Entrainmen t Study - Fish and Invertebrate Abundances Survey: HGSEA24 Start Date: 11/29/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 38 119.26 Genyonemus lineatus white croaker 4 12.55 Sciaenidae unid

. croakers 2 6.33 Hypsoblennius spp . combtooth blennies 2 5.57 larval fish fragment unidentified larval fishes 1 3.77 Paralichthys californicus California halibut 1 3.77 Lepidogobius lepidus bay goby 1 3.67 larval fish

- damaged unidentified larval fi shes 1 3.17 Gillichthys mirabilis longjaw mudsucker 1 2.89 Engraulis mordax northern anchovy 1 2.86 larvae, unidentified yolksac unidentified yolksac larvae 1 2.86 Pleuronectidae unid. righteye flounders 1 2.86 Total Fishes: 54 Eggs fish eggs unid. unidentified fish eggs 19 118.35 Paralichthyidae unid. (eggs) sand flounder eggs 5 26.71 Sciaenidae unid. (eggs) croaker eggs 4 21.53 Sciaen. / Paralich. / Labridae (eggs) fish eggs 3 20.67 Pleuronichthys spp. (eggs) turbot eggs 2 13.74 Total Eggs: 33 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 136 411.98 Majidae unid. (megalops) spider crab megalops 29 92.35 Pinnixa spp. (megalops) pea crabs megalops 8 23.68 Grapsidae unid. (megalops) shore crab megalops 4 11.31 Brachyura unid. (megalops) unidentified crab megalops 1 2.78 Total Target Invertebrates: 178

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 61 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA25 Start Date: 12/11/2006 Stations: E1 Mean Concentration Taxon Commo n Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 248 816.74 Genyonemus lineatus white croaker 36 111.05 Engraulis mordax northern anchovy 13 44.24 larvae, unidentified yolksac unidentified yolksac larvae 3 9.33 Hypsoblennius spp . combtooth bl ennies 1 3.05 Sciaenidae unid. croakers 1 3.05 Seriphus politus queenfish 1 3.05 Gibbonsia spp . clinid kelpfishes 1 3.04 Gillichthys mirabilis longjaw mudsucker 1 2.56 Total Fishes: 305 Eggs fish eggs unid. unidentified fish eggs 79 517.41 Sciaeni dae unid. (eggs) croaker eggs 31 194.43 Sciaen. / Paralich. / Labridae (eggs) fish eggs 3 20.64 Pleuronichthys spp. (eggs) turbot eggs 3 18.98 Paralichthyidae unid. (eggs) sand flounder eggs 2 14.11 Total Eggs: 118 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 145 462.71 Majidae unid. (megalops) spider crab megalops 27 83.62 Pinnixa spp. (megalops) pea crabs megalops 15 46.96 Paguridae unid. (megalops) hermit crab megalops 1 2.56 Total Target Invertebrates: 188

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 62 Harbo r Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA25 Start Date: 12/11/2006 Stations: H1 - H3 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 197 348.46 Gobiidae u nid. gobies 154 279.52 Engraulis mordax northern anchovy 27 50.15 Lepidogobius lepidus bay goby 14 26.93 larvae, unidentified yolksac unidentified yolksac larvae 8 14.65 larval fish fragment unidentified larval fishes 4 7.05 Hypsoblennius spp . combtoo th blennies 3 6.19 Paralichthys californicus California halibut 3 4.96 larval fish

- damaged unidentified larval fishes 2 3.63 Pleuronichthys ritteri spotted turbot 1 1.80 Citharichthys stigmaeus speckled sanddab 1 1.72 Pleuronichthys guttulatus diamo nd turbot 1 1.46 Total Fishes: 415 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 243 444.14 Pinnixa spp. (megalops) pea crabs megalops 44 78.78 Majidae unid. (megalops) spider crab megalops 23 42.16 Pinnotheridae (megalops) pea c rab megalops 18 33.30 Brachyura unid. (megalops) unidentified crab megalops 7 12.35 Cancer anthonyi (megalops) yellow crab megalops 2 3.39 Grapsidae unid. (megalops) shore crab megalops 1 2.42 Total Target Invertebrates: 338

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 63 Harbor Generating Station Entrainment Study - Fish and Invertebrate Abundances Survey: HGSEA25 Start Date: 12/11/2006 Stations: H4 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 149 752.62 Gobiidae unid. gobies 49 244.49 Engraulis mordax northern anchovy 5 26.79 larvae, unidentified yolksac unidentified yolksac larvae 5 23.99 Paralichthys californicus California halibut 3 13.91 larval fish

- damaged unidentified larval fishes 1 4.84 Pleuronichthys guttulatus di amond turbot 1 4.84 Clinocottus analis wooly sculpin 1 4.64 Engraulidae unid. anchovies 1 4.64 Total Fishes: 215 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 142 707.01 Pinnixa spp. (megalops) pea crabs megalops 15 72.16 Majidae unid. (megalops) spider crab megalops 11 58.90 Grapsidae unid. (megalops ) shore crab megalops 5 25.52 Pinnotheridae (megalops) pea crab megalops 2 9.47 Total Target Invertebrates: 175

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 64 Harbor Generating Station Entrainment Study - Fish and I nvertebrate Abundances Survey: HGSEA25 Start Date: 12/11/2006 Stations: H5 - H6 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Genyonemus lineatus white croaker 340 935.02 Gobiidae unid. gobies 59 166.88 Engraulis mordax northern anchovy 11 31.86 larvae, unidentified yolksac unidentified yolksac larvae 9 27.55 Paralichthys californicus California halibut 6 16.85 Hypsoblennius spp . combtooth blennies 5 14.11 Pleuronichthys guttulatus diamond turbot 4 9.93 Lepidogobius lepidus bay goby 3 8.45 larval fish

- damaged unidentified larval fishes 3 8.32 larval fish fragment unidentified larval fishes 2 5.83 Citharichthys stigmaeus speckled sanddab 1 3.35 Ruscarius creaseri roughcheek sculpin 1 3.13 Atherinopsidae unid. silversides 1 2.91 Pleuronichthys ritteri spotted turbot 1 2.82 Seriphus politus queenfish 1 2.05 Total Fishes: 447 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 125 352.15 Pinnixa spp. (megalops) pea crabs megalops 31 79.30 Majidae unid. (me galops) spider crab megalops 18 54.02 Brachyura unid. (megalops) unidentified crab megalops 11 33.88 Pinnotheridae (megalops) pea crab megalops 3 7.11 Total Target Invertebrates: 188

### Harbor Generating Statio n

IM&E Final Report Appendix C1: Entrainment Data

C1 - 65 Harbor Generating Station Entrainment Study - Fish and Inve rtebrate Abundances Survey: HGSEA26 Start Date: 12/26/2006 Stations: E1 Mean Concentration Taxon Common Name Count (#/1,000m 3 ) Fishes Gobiidae unid. gobies 111 480.84 Genyonemus lineatus white croaker 4 17.10 Lepidogobius lepidus bay goby 3 13.52 Engraulis mordax northern anchovy 2 9.25 Sciaenidae unid. croakers 2 8.87 Typhlogobius californiensis blind goby 1 4.98 Total Fishes: 123 Eggs fish eggs unid. unidentified fish eggs 13 113.01 Paralichthyidae unid. (eggs) sand flounder eggs 4 31.97 Total Eggs: 17 Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 7 29.14 Majidae unid. (megalops) spider crab megalops 2 8.94 Pinnixa spp. (megalops) pea crabs megalops 2 8.94 Total Target Invertebrates: 11

### Harbor Generating Station Appendix C2: Calculated Annual

IM&E Final Report Entrainment and Standard Error Data

C2 - 1 Appendix D2. Cal culated Annual Entrainment and Standard Error Data Table D2

- 1. Calculated total annual entrainment and standard error of larval fish and eggs at HGS in 2006 based on actual and design cooling water intake flow volumes.

Taxon Common Name Calculated Annua l Entrainment (Actual Flows) Standard Error Calculated Annual Entrainment (Design Flows) Standard Error Larval Fish Gobiidae unid. gobies 33,290,815 869,904 75,938,007 1,933,837 Acanthogobius flavimanus yellowfin goby 15,407,999 1,744,298 37,604,33 6 4,325,986 Genyonemus lineatus white croaker 7,164,843 154,612 18,777,752 401,199 Lepidogobius lepidus bay goby 2,376,260 66,436 5,070,071 138,207 Hypsoblennius spp . combtooth blennies 2,255,907 55,768 4,362,576 100,491 Sciaenidae unid. croakers 995,4 38 43,751 2,856,932 173,898 Engraulidae unid. anchovies 940,784 54,597 2,068,979 105,000 unidentified fish, damaged unidentified damaged fish 646,175 25,303 1,571,226 58,358 larvae, unidentified yolksac unidentified yolksac larvae 288,308 22,120 679,015 48,803 Gillichthys mirabilis longjaw mudsucker 254,865 12,241 558,887 27,666 Gobiesocidae unid. clingfishes 236,654 16,157 515,917 35,120 Icelinus spp . sculpins 190,484 16,231 446,021 33,925 Paralichthys californicus California halibut 165,782 10,324 424,529 24,336 Paralabrax spp . sand bass 122,010 26,030 271,192 56,085 Cheilotrema saturnum black croaker 94,525 16,173 200,992 34,434 Seriphus politus queenfish 83,731 7,804 176,487 15,642 Gibbonsia spp . clinid kelpfishes 77,308 7,719 173,122 17,783 Rhinogobiops nicholsi blackeye goby 71,631 6,642 145,314 12,681 Leptocottus armatus Pacific staghorn sculpin 68,768 6,698 160,195 15,742 Semicossyphus pulcher California sheephead 64,438 6,322 141,346 13,412 larval/post

- larval fish unid. larval fishes 5 8,152 6,794 119,578 13,236 Clinocottus spp . sculpins 55,826 6,271 112,731 12,202 Citharichthys spp . sanddabs 41,222 5,876 115,405 15,092 Pleuronichthys spp . turbots 36,951 4,803 73,078 9,001 Pleuronichthys guttulatus diamond turbot 36,799 4,992 93,021 11,151 (table continued)

### Harbor Generating Station Appendix C2: Calculated Annual

IM&E Final Report Entrainment and Standard Error Data

C2 - 2 Table D2

- 1 (continued). Calculated total annual entrainment and standard error of larval fish and eggs at HGS in 2006 based on actual and design cooling water intake flow volumes.

Taxon Common Name Calculated Annual Entrainment (Actual Flows) Standard Error Calculated Annual Entrainment (Design Flows) Standard Error Labrisomidae unid. labrisomid blennies 29,483 5,990 71,609 14,662 Oxyjulis californica senorita 24,673 3,716 52,620 7,495 Pleuronectidae unid. righteye flounders 23,120 3,732 52,521 8,234 Ruscarius creaseri roughcheek sculpin 20,616 4,081 50,242 9,975 Pleuronectiformes unid. flatfishes 16,347 2,475 40,493 6,128 Cottidae unid. sculpins 15,756 3,158 31,607 6,023 Lythrypnus zebra zebra goby 14,795 2,839 34,416 6,5 07 Syngnathus spp . pipefishes 14,250 3,935 20,766 5,550 Orthonopias triacis snubnose sculpin 13,806 2,341 32,367 5,428 Sardinops sagax Pacific sardine 11,836 1,898 29,933 4,789 Artedius spp. sculpins 10,653 2,986 17,916 4,788 Typhlogobius californiens is blind goby 10,523 2,919 26,455 7,337 Clupeidae unid. herrings 8,864 2,121 36,966 6,990 Roncador stearnsi spotfin croaker 8,735 2,336 21,531 5,754 Pleuronichthys verticalis hornyhead turbot 8,693 2,398 19,596 5,237 Merluccius productus Pacific hake 8 ,421 2,362 19,741 5,475 Girella nigricans opaleye 7,894 2,114 19,963 5,335 Bathylagidae unid. blacksmelt 6,622 1,827 14,928 3,990 Lythrypnus spp. gobies 6,249 1,733 11,481 3,069 Bathymasteridae unid. ronquils 5,843 1,621 14,511 4,025 Paralichthyidae u nid. sand flounders 1,926 1,024 20,449 5,465 Chitonotus / Icelinus sculpins 1,798 957 19,097 5,104 Atherinopsidae unid. silversides 1,422 756 15,096 4,035 Total Larval Fish 65,297,999 153,331,011 Fish Eggs fish eggs unid. unidentified fish egg s 49,261,253 2,104,741 130,894,994 5,066,941 Genyonemus lineatus white croaker eggs 17,867,461 901,215 43,114,182 2,226,295 Sciaenidae unid. croaker eggs 14,562,519 607,258 41,351,239 1,519,856 (table continued)

### Harbor Generating Station Appendix C2: Calculated Annual

IM&E Final Report Entrainment and Standard Error Data

C2 - 3 Table D2

- 1 (continued). Calculated total annual entrainment and standard error of larval fish and eggs at HGS in 2006 based on actual and design cooling water intake flow volumes.

Taxon Common Name Calculated Annual Entrainment (Actual Flows) Standard Error Calculated Annual Entrainment (Desi gn Flows) Standard Error Fish Eggs Paralichthyidae unid. sand flounder eggs 8,780,223 214,000 30,684,631 563,025 Sciaenidae/Paralichthyidae/Labridae SPL fish eggs 5,208,682 159,806 11,085,521 362,938 Citharichthys spp. sanddab eggs 1,499,868 124 ,838 5,319,639 589,511 Pleuronichthys spp. turbot eggs 1,018,933 29,538 2,987,271 72,517 Paralichthys californicus California halibut eggs 860,518 39,298 2,013,440 92,977 Engraulidae unid. anchovy eggs 803,290 23,311 1,932,617 58,079 Atherinopsidae unid. silverside eggs 22,147 6,143 40,690 10,875 Total Fish Eggs 99,884,894 269,424,225

### Harbor Generating Station Appendix C2: Calculated Annual

IM&E Final Report Entrainment and Standard Error Data

C2 - 4 Table D2

- 2. Calculated total annual entrainment and standard error of target shellfishes at HGS in 2006 based on actual and design cooling water intake flow volu mes.

Taxon Common Name Calculated Annual Entrainment (Actual Flows) Standard Error Calculated Annual Entrainment (Design Flows) Standard Error Target Invertebrates Pugettia spp. (megalops) kelp crabs megalops 12,009,598 285,695 26,357,647 608,953 Majidae unid. (megalops) spider crab megalops 3,520,320 149,419 7,285,587 275,123 Pinnixa spp. (megalops) pea crabs megalops 1,810,300 106,592 4,467,908 287,416 unidentified crab (megalops) unidentified crab megalops 727,346 40,778 1,480,727 75,784 Gr apsidae unid. (megalops) shore crab megalops 241,523 12,455 526,881 26,937 Panulirus interruptus (phyllosome) California spiny lobster (larval) 217,477 16,512 360,853 25,703 Paguridae unid. (megalops) hermit crab megalops 165,787 12,935 422,270 31,713 B rachyura unid. (megalops) unidentified crab megalops 64,917 7,928 134,388 15,948 Pinnotheres spp. (megalops) pea crab megalops 38,804 4,466 84,172 9,695 Lophopanopeus spp. (megalops) black

- clawed crab megalops 27,081 4,549 51,987 8,228 Loligo opalescens market squid 26,676 4,612 62,179 10,354 Cancer spp. (megalops) cancer crabs megalops 15,625 2,506 35,225 5,474 Grapsidae / Cancridae (megalops) unidentified crab megalops 12,015 3,213 21,990 5,678 Pinnotheridae (megalops) pea crab megalops 9,749 2,607 17,843 4,607 Diogenidae (megalops) left

- handed hermit crabs megalops 7,894 2,114 19,963 5,335 Pachygrapsus crassipes (megalops) striped shore crab megalops 6,224 1,727 15,456 4,287 Total 18,901,336 41,345,075

# Harbor Generating Station



# Appendix D Impingement Data

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 2 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI1 Start Date: January 5, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 2 0.014

Embiotoca jacksoni black perch 2 0.029 Heterostichus rostratus giant kelpfish 3 0.497 Tridentiger trigonocephalus chameleon goby 1 0.002 Acanthogobius flavimanus yellowfin goby 1 0.014 9 0.556
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.003 Octopus bimaculatus/bimaculoides California two

- spot octopus 6 1.268 Heptacarpus paludicola California coastal shrimp 2 0.002 Panulirus interruptus California spiny lobster 2 0.312 13 1.586

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 3 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI2 Start Date: January 12, 2006 Survey Totals

Taxon Common Name Abundance Bioma ss (kg) Fish Cymatogaster aggregata shiner perch 2 0.011

Embiotoca jacksoni black perch 2 0.030 Gibbonsia elegans spotted kelpfish 1 0.008 Heterostichus rostratus giant kelpfish 2 0.370 Urobatis halleri round stingray 1 0.165 Acanthogobius flavimanus yellowfin goby 1 0.029 Rhacochilus vacca pile perch 1 0.024 10 0.637
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.003

Octopus bimaculatus/bimaculoides California two

- spot octopus 2 0.237 5 0.240
N on - Shellfish Cnidaria sea jelly, unid. 1 0.001

Parastichopus parvimensis warty sea cucumber 1 0.027 2 0.028

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -4 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI3 Start Date: January 19, 2006
S urvey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.023

Gibbonsia elegans spotted kelpfish 1 0.010 Urobatis halleri round stingray 1 0.181 Tridentiger trigonocephalus chameleon goby 1 0.005 Paralabrax nebulifer barred sand bass 1 0.004 5 0.223
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 1 0.003 Octopus bimaculatus/bimaculoides California two

- spot octopus 1 0.249 Heptacarpus paludicola California coastal shrimp 5 0.003 8 0.256

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 5 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI4 Start Date: January 26, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 2 0.025

Phanerodon furcatus white seaperch 1 0.028 Urobatis halleri round stingray 2 0.659 Acanthogobius flavimanus yellowfin goby 1 0.018 6 0.730
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Octopus bimaculatus/bimaculoides California two

- spot octopus 1 0.240 Heptacarpus paludicola California coastal shrimp 1 0.001 Panulirus interruptus California spiny lobster 1 0.199 4 0.441
Non

- Shellfish Archidoris montereyensis Monterey sea

- lemon 1 0.012

Parastichopus californicus California sea cucumber 1 0.013 Cuthona lagunae orange - faced nudibranch 1 0.001 3 0.026

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 6 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: H GSFI5 Start Date: February 2, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 4 0.076

Gibbonsia elegans spotted kelpfish 1 0.004 Heterostichus rostratus giant kelpfish 1 0.011 Tridentiger trigonocephalus chameleon goby 1 0.008 Acanthogobius flavimanus yellowfin goby 6 0.122 13 0.221
Shellfish Octopus bimaculatus/bimaculoides California two

- spot octopus 1 0.152

Heptacarpus paludicola California coastal shrimp 1 0.001 Lysmata californica red rock shrimp 2 0.002 Panulirus interruptus California spiny lobster 3 2.129 7 2.284
Non

- Shellfish Triopha maculata spotted triopha 1 0.001 1 0.001

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 7 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI6 Start Date: February 9, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish None
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Hemigrapsus oregonensis yellow shore crab 1 0.002 Pyromaia tuberculata tuberculate pear crab 1 0.003 3 0.006
Non

- Shellfish Diaulula sandiegensis ring

- spotted dorid 1 0.002

Pisaster sp sea star, unid. 1 0.143 Parastichopus parvimensis warty sea cuc umber 1 0.133 3 0.278

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 8 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI7 Start Date: February 16, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata s hiner perch 1 0.007

Embiotoca jacksoni black perch 1 0.023 Gibbonsia elegans spotted kelpfish 1 0.006 Heterostichus rostratus giant kelpfish 2 0.037 Urobatis halleri round stingray 1 0.137 Acanthogobius flavimanus yellowfin goby 1 0.017 7 0.2 27
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 1 0.001 Octopus bimaculatus/bimaculoides California two

- spot octopus 4 1.023 6 1.025
Non

- Shellfish Hermissenda crassicornis hermissenda 1 0.001

Pycnogonida sea spider, unid. 1 0.001 2 0.002

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 9 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI8 Start Date: February 23, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 1 0.003

Embiotoca jacksoni black perch 1 0.024 Heterostichus rostratus giant kelpfish 1 0.025 Urobatis halleri round stingray 1 0.348 Acanthogobius flavimanus yellowfin goby 2 0 .032 6 0.432
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.002

Pyromaia tuberculata tuberculate pear crab 2 0.002 Heptacarpus paludicola California coastal shrimp 1 0.001 Panulirus interruptus California spiny lobster 1 0.432 6 0.437
Non

- Shellfish Cnidaria sea jelly, unid. 1 0.028

Holothuroidea sea cucumber, unid. 1 0.001 Pleurobranchaea californica California sea slug 1 1.778 3 1.807

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 10 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI9 Start Date: March 2, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Gibbonsia elegans spotted kelpfish 1 0.013

Urobatis halleri round stingray 1 0.217 2 0.230
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.005

Pyromaia tuberculata tuberculate pear crab 2 0.002 Octopus bimaculatus/bimaculoides California two

- spot octopus 1 0.313 Heptacarpus paludicola California coastal shrimp 1 0.001 Cancer ante nnarius Pacific rock crab 1 0.335 Heptacarpus sp coastal shrimp, unid. 2 0.002 10 0.658
Non

- Shellfish Hermissenda crassicornis hermissenda 1 0.001

Diaulula sandiegensis ring

- spotted dorid 1 0.003 2 0.004

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 11 Harbor Generating Statio n - Normal Operation Impingement Data

- Fish Survey: HGSFI10 Start Date: March 9, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Urobatis halleri round stingray 3 0.739

Tridentiger trigonocephalus chameleon goby 1 0.006 Acanthogobius flavimanus yellowfin goby 1 0.019 5 0.764
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.005 Octopus bimaculatus/bimaculoides California two

- spot octopus 2 0.419 Heptacarpus sp coastal shrimp, unid. 1 0.001 7 0.426
Non

- Shellfish Navanax inermis California aglaja 1 0.031 1 0.031

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 12 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI11 S tart Date: March 16, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Acanthogobius flavimanus yellowfin goby 1 0.026 1 0.026
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Hemigra psus oregonensis yellow shore crab 1 0.005 Pyromaia tuberculata tuberculate pear crab 2 0.003 Heptacarpus paludicola California coastal shrimp 2 0.002 Heptacarpus sp coastal shrimp, unid. 1 0.001 7 0.012
Non

- Shellfish Pisaster sp sea star, unid. 1 1.194

Discodorididae nudibranch, unid. 1 0.002 Anisodoris nobilis Pacific sea

- lemon 1 0.001 3 1.197

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 13 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI12 Start Date: March 23, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Genyonemus lineatus white croaker 1 0.022

Gibbonsia elegans spotted kelpfish 1 0.010 Porichthys notatus plainfin midshipman 1 0.003 3 0.035
Shellfish Heptacarpus pa lpator intertidal coastal shrimp 2 0.002

Pyromaia tuberculata tuberculate pear crab 1 0.001 Panulirus interruptus California spiny lobster 1 0.772 4 0.775
Non

- Shellfish Dendronotus iris giant

- frond

- aeolis 1 0.019

Parastichopus parvim ensis warty sea cucumber 1 0.047 Ammothea hilgendorfi sea spider 3 0.002 Scrippsia pacifica giant bell jelly 1 0.044 Ctenophora comb jelly, unid. 1 0.006 7 0.118

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 14 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HG SFI13 Start Date: March 30, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.126

Heterostichus rostratus giant kelpfish 1 0.118 Urobatis halleri round stingray 2 0.587 Acan thogobius flavimanus yellowfin goby 2 0.019 Icelinus quadriseriatus yellowchin sculpin 1 0.005 7 0.855
Shellfish Portunus xantusii Xantus swimming crab 1 0.035

Pyromaia tuberculata tuberculate pear crab 1 0.003 Octopus bimaculatus/bi maculoides California two

- spot octopus 2 0.334 Panulirus interruptus California spiny lobster 1 0.345 5 0.717
Non

- Shellfish Hermissenda crassicornis hermissenda 2 0.001

Anisodoris nobilis Pacific sea

- lemon 1 0.009 3 0.010

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 15 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI14 Start Date: April 6, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.005

Porichthys notatus pl ainfin midshipman 1 0.001 Embiotocidae surfperch, unid. 1 0.004 3 0.010
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.007 3 0.008
Non

- Shellfish Hermisse nda crassicornis hermissenda 1 0.001

Aplysia californica California seahare 1 0.118 Anisodoris nobilis Pacific sea

- lemon 1 0.012 3 0.131

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 16 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI15 Start Date: April 13, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 1 0.025

Embiotoca jacksoni black perch 7 0.049 Pleuronichthys verticalis hornyhead turbot 1 0.089 Porichthys myriaster sp ecklefin midshipman 1 0.003 Acanthogobius flavimanus yellowfin goby 2 0.039 Rhacochilus vacca pile perch 1 0.059 13 0.264
Shellfish Pyromaia tuberculata tuberculate pear crab 3 0.005

Heptacarpus paludicola California coastal shrimp 1 0.001 4 0.006
Non

- Shellfish Hermissenda crassicornis hermissenda 4 0.002

Pycnogonida sea spider, unid. 1 0.001 Parastichopus parvimensis warty sea cucumber 1 0.007 Parastichopus sp sea cucumber, unid. 1 0.001 7 0.011

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 17 Harbor G enerating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI16 Start Date: April 20, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Atherinops affinis topsmelt 1 0.029

Embiotoca jacksoni black perch 1 0.005 2 0.034
Shellfish Pyromaia tuberculata tuberculate pear crab 1 0.003

Octopus bimaculatus/bimaculoides California two

- spot octopus 2 0.287 Heptacarpus paludicola California coastal shrimp 1 0.001 4 0.291
Non

- She llfish Hermissenda crassicornis hermissenda 5 0.002

Polycera hedgpethi Hedgpeth's polycera 1 0.001 Leptopecten sp scallop, unid. 4 0.001 Pycnogonida sea spider, unid. 1 0.001 Parastichopus sp sea cucumber, unid. 1 0.001 12 0.006

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 18 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI19 Start Date: May 11, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 5 0.072

Embiotoca jackson i black perch 8 0.041 Gibbonsia elegans spotted kelpfish 2 0.013 Heterostichus rostratus giant kelpfish 1 0.024 Pleuronichthys verticalis hornyhead turbot 1 0.079 Porichthys myriaster specklefin midshipman 60 0.521 Porichthys notatus plainfin mid shipman 6 0.023 Syngnathus leptorhynchus bay pipefish 1 0.006 Urobatis halleri round stingray 1 0.625 Tridentiger trigonocephalus chameleon goby 1 0.007 Acanthogobius flavimanus yellowfin goby 3 0.036 Paralabrax nebulifer barred sand bass 1 0.007 90 1.454
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.004 Heptacarpus paludicola California coastal shrimp 1 0.001 4 0.006
Non

- Shellfish Aplysia calif ornica California seahare 1 0.177 Diaulula sandiegensis ring

- spotted dorid 1 0.001 Ammothea hilgendorfi sea spider 2 0.001 4 0.179

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 19 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI20 Start Date: May 18, 200 6 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 4 0.039

Embiotoca jacksoni black perch 16 0.083 Heterostichus rostratus giant kelpfish 1 0.039 Porichthys myriaster specklefin mi dshipman 5 0.033 Paralabrax nebulifer barred sand bass 2 0.017 28 0.211
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.002

Hemigrapsus oregonensis yellow shore crab 1 0.008 Pyromaia tuberculata tuberculate pear crab 4 0 .006 Heptacarpus paludicola California coastal shrimp 2 0.002 Pandalus danae dock shrimp 1 0.006 10 0.024
Non

- Shellfish Hermissenda crassicornis hermissenda 2 0.002

Parastichopus parvimensis warty sea cucumber 1 0.012 3 0.014

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 20 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI21 Start Date: May 25, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.007

Heterostichus ro stratus giant kelpfish 1 0.024 Phanerodon furcatus white seaperch 3 0.027 Pleuronichthys verticalis hornyhead turbot 1 0.077 Porichthys notatus plainfin midshipman 1 0.007 Paralabrax nebulifer barred sand bass 1 0.139 8 0.281
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.002

Panulirus interruptus California spiny lobster 1 0.149 3 0.151
Non

- Shellfish Hermissenda crassicornis hermissenda 3 0.002

Parastichopus sp sea cucumber, unid. 1 0.001 4 0. 003

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 21 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI22 Start Date: June 1, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 20 0.256

Emb iotoca jacksoni black perch 18 0.235 Gibbonsia elegans spotted kelpfish 1 0.010 Leptocottus armatus Pacific staghorn sculpin 2 0.013 Phanerodon furcatus white seaperch 8 0.183 Porichthys myriaster specklefin midshipman 3 0.203 Urobatis halleri ro und stingray 1 0.672 Paralabrax nebulifer barred sand bass 1 0.005 54 1.577
Shellfish Pyromaia tuberculata tuberculate pear crab 2 0.004 2 0.004
Non

- Shellfish Hermissenda crassicornis hermissenda 22 0.005

Polycera atra orange - spike polycera 1 0.001 Pycnogonida sea spider, unid. 5 0.002 Parastichopus californicus California sea cucumber 1 0.018 29 0.026

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 22 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI23 Start Date: June 8, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 3 0.033

Embiotoca jacksoni black perch 3 0.011 Heterostichus rostratus giant kelpfish 2 0.104 Phanerodon furcatus white seaperch 1 0.002 Paralabrax nebulifer barred sand bass 1 0.016 10 0.166
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.003

Panulirus interruptus California spiny lobster 2 0.496 5 0.499
Non

- Shellfish Hermiss enda crassicornis hermissenda 32 0.008

Polycera atra orange - spike polycera 2 0.001 Flabellina trilineata threeline aeolis 1 0.001 Dendronotus frondosus leafy dendronotid 2 0.001 Triopha maculata spotted triopha 1 0.002 Parastichopus parvimensis w arty sea cucumber 2 0.345 40 0.358

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 23 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI24 Start Date: June 15, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jack soni black perch 5 0.017 Gibbonsia elegans spotted kelpfish 3 0.025 Hyperprosopon argenteum walleye surfperch 1 0.006 Phanerodon furcatus white seaperch 1 0.117 Porichthys myriaster specklefin midshipman 2 0.173 12 0.338
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.005 Cancer antennarius Pacific rock crab 1 0.324 Pandalus danae dock shrimp 1 0.001 5 0.331
Non

- Shellfish Hermissenda crassicornis hermissenda 52 0.015

Polycera hedgpethi Hedgpeth's polycera 2 0.001 Polycera atra orange - spike polycera 10 0.004 Flabellina trilineata threeline aeolis 1 0.001 Dirona picta spotted dirona 6 0.002 Triopha maculata spotted triopha 5 0.002 76 0. 025

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 24 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI25 Start Date: June 22, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Heterostichus rostratus giant kelpfish 1 0.084

Pleuronichthys verticalis hornyhead turbot 1 0.220 Urobatis halleri round stingray 1 0.347 Tridentiger trigonocephalus chameleon goby 1 0.007 4 0.658
Shellfish Crangon nigromaculata blackspotted bay shrimp 1 0.001

Heptacarpus palpato r intertidal coastal shrimp 4 0.003 Pyromaia tuberculata tuberculate pear crab 5 0.010 Panulirus interruptus California spiny lobster 1 0.238 11 0.252
Non

- Shellfish Hermissenda crassicornis hermissenda 36 0.012

Polycera atra orange - s pike polycera 1 0.001 Leptopecten sp scallop, unid. 1 0.001 Pycnogonida sea spider, unid. 6 0.002 44 0.016

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 25 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI26 Start Date: June 29, 2006 Survey T otals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 15 0.021

Embiotoca jacksoni black perch 6 0.027 Engraulis mordax northern anchovy 1 0.001 Genyonemus lineatus white croaker 1 0.001 Heterostichus rost ratus giant kelpfish 1 0.056 Phanerodon furcatus white seaperch 3 0.033 Pleuronichthys verticalis hornyhead turbot 1 0.116 28 0.255
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.002

Pyromaia tuberculata tuberculate pear crab 13 0.007 Cancer antennarius Pacific rock crab 1 0.416 Panulirus interruptus California spiny lobster 1 0.112 17 0.537
Non

- Shellfish Hermissenda crassicornis hermissenda 45 0.014

Leptopecten sp scallop, unid. 2 0.001 Flabelli na trilineata threeline aeolis 1 0.001 Dirona picta spotted dirona 1 0.001 Pycnogonida sea spider, unid. 14 0.002 Parastichopus parvimensis warty sea cucumber 1 0.042 Parastichopus californicus California sea cucumber 1 0.035 65 0.096

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 26 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI27 Start Date: July 6, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 5 0.130

Genyonemus lineatus wh ite croaker 2 0.003 Gibbonsia elegans spotted kelpfish 1 0.009 Syngnathus leptorhynchus bay pipefish 1 0.002 Urobatis halleri round stingray 4 0.797 13 0.941
Shellfish Portunus xantusii Xantus swimming crab 1 0.040

Pyromaia tubercu lata tuberculate pear crab 5 0.006 Heptacarpus paludicola California coastal shrimp 1 0.001 Panulirus interruptus California spiny lobster 1 0.343 8 0.390
Non

- Shellfish Hermissenda crassicornis hermissenda 18 0.006

Leptopecten sp scallop, unid. 1 0.005 Aplysia sp seahare, unid. 1 0.310 Pycnogonida sea spider, unid. 3 0.002 23 0.323

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 27 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI28 Start Date: July 13, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 1 0.001

Embiotoca jacksoni black perch 2 0.007 Engraulis mordax northern anchovy 2 0.001 Phanerodon furcatus white seaperch 1 0.001 Porichthys myriaster spe cklefin midshipman 1 0.444 Urobatis halleri round stingray 2 0.708 Paralabrax nebulifer barred sand bass 1 0.012 10 1.174
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.002

Pyromaia tuberculata tuberculate pear crab 4 0 .002 Cancer antennarius Pacific rock crab 1 0.295 Panulirus interruptus California spiny lobster 1 0.249 9 0.548
Non

- Shellfish Hermissenda crassicornis hermissenda 5 0.003

Leptopecten sp scallop, unid. 1 0.001 Diaulula sandiegensis ring

- spotted dorid 1 0.003 Pycnogonida sea spider, unid. 1 0.001 Parastichopus parvimensis warty sea cucumber 3 0.318 11 0.326

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 28 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI29 Start Date: July 20, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 4 0.007

Engraulis mordax northern anchovy 1 0.001 Urobatis halleri round stingray 8 1.599 Rhacochilus vacca pile perch 2 0.339 15 1.946
Shellfish Portunus xantusii Xantus swimming crab 1 0.024

Pyromaia tuberculata tuberculate pear crab 20 0.013 Farfantepenaeus californiensis yellowleg shrimp 1 0.006 Panulirus interruptus California spiny lobster 2 0.422 24 0 .465
Non

- Shellfish Hermissenda crassicornis hermissenda 10 0.002

Triopha maculata spotted triopha 2 0.001 Pycnogonida sea spider, unid. 28 0.002 Parastichopus parvimensis warty sea cucumber 2 0.154 Parastichopus californicus California sea cucumber 1 0.026 43 0.185

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 29 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI30 Start Date: July 27, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Gibbonsia elegans spotted kelpfish 1 0.014

Heterostichus rostratus giant kelpfish 3 0.195 Urobatis halleri round stingray 11 2.052 Paralabrax nebulifer barred sand bass 3 0.024 18 2.285
Shellfish Heptacarpus palpator intertidal coastal shrimp 4 0.002

Pyromaia tuberculata tuberculate pear crab 8 0.007 Panulirus interruptus California spiny lobster 2 0.379 14 0.388
Non

- Shellfish Hermissenda crassicornis hermissenda 14 0.005

Aplysia californica California seahare 1 0.563 Triopha maculata spotted triopha 1 0.001 Diaulula sandiegensis ring

- spotted dorid 4 0.002 Pycnogonida sea spider, unid. 25 0.003 45 0.574

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 30 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI31 Start Date: August 3, 200 6 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Heterostichus rostratus giant kelpfish 1 0.098

Porichthys myriaster specklefin midshipman 1 0.327 Urobatis halleri round stingray 45 9.318 Paralabrax nebulifer barre d sand bass 1 0.301 48 10.044
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.001

Pyromaia tuberculata tuberculate pear crab 7 0.013 Panulirus interruptus California spiny lobster 5 0.924 14 0.938
Non

- Shellfish Hermissenda crassicornis hermissenda 6 0.002

Triopha maculata spotted triopha 1 0.001 Diaulula sandiegensis ring

- spotted dorid 1 0.002 Pycnogonida sea spider, unid. 26 0.002 Holothuroidea sea cucumber, unid. 1 0.003 35 0.010

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 31 Harbor Gen erating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI32 Start Date: August 10, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 3 0.017

Embiotoca jacksoni black perch 1 0.340 Gibbonsia elegans spotted kelpfish 1 0.009 Urobatis halleri round stingray 48 9.878 Paralabrax nebulifer barred sand bass 2 0.035 55 10.279
Shellfish Pyromaia tuberculata tuberculate pear crab 11 0.015

Panuliru s interruptus California spiny lobster 7 1.113 Pandalus stenolepis roughpatch shrimp 1 0.002 19 1.130
Non

- Shellfish Hermissenda crassicornis hermissenda 3 0.002

Aurelia aurita moon jelly 1 0.010 Leptopecten sp scallop, unid. 2 0.002 Triopha maculata spotted triopha 1 0.001 Diaulula sandiegensis ring

- spotted dorid 1 0.001 Pycnogonida sea spider, unid. 100 0.002 Parastichopus parvimensis warty sea cucumber 1 0.046 109 0.064

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 32 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI33 Start Date: August 17, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Heterostichus rostratus giant kelpfish 1 0.008

Urobatis halleri round stingray 21 4.670 22 4.678
Shellfish Crangon nigromaculata blackspotted bay shrimp 1 0.001

Heptacarpus palpator intertidal coastal shrimp 2 0.001 Pyromaia tuberculata tuberculate pear crab 2 0.001 Panulirus interruptus California spiny lobster 5 0.971 Pinnixa s p pea crab, unid. 1 0.001 11 0.975
Non

- Shellfish Hermissenda crassicornis hermissenda 1 0.001

Triopha maculata spotted triopha 3 0.002 Diaulula sandiegensis ring

- spotted dorid 5 0.004 Parastichopus californicus California sea cucumbe r 1 0.133 10 0.140

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 33 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI34 Start Date: August 24, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 1 0.002

Gibbonsia elegans spotted kelpfish 1 0.017 Heterostichus rostratus giant kelpfish 1 0.025 Urobatis halleri round stingray 45 9.163 Paralabrax nebulifer barred sand bass 2 0.030 50 9.237
Shellfish Heptacarpus palpato r intertidal coastal shrimp 2 0.002

Pyromaia tuberculata tuberculate pear crab 1 0.001 Panulirus interruptus California spiny lobster 11 2.301 14 2.304
Non

- Shellfish Hermissenda crassicornis hermissenda 2 0.001 2 0.001

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 34 Harbor Ge nerating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI35 Start Date: August 31, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Gibbonsia elegans spotted kelpfish 1 0.021

Heterostichus rost ratus giant kelpfish 1 0.420 Urobatis halleri round stingray 34 7.159 Paralabrax nebulifer barred sand bass 3 0.036 39 7.636
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.004 Panulirus interruptus California spiny lobster 18 4.036 Heptacarpus sp coastal shrimp, unid. 1 0.001 Heptacarpus stimpsoni Stimpson coastal shrimp 1 0.001 23 4.043
Non

- Shellfish Hermissenda crassicornis hermissenda 4 0.002

Triopha maculata spotted triopha 1 0.001 Pycnogonida sea spider, unid. 2 0.001 Parastichopus californicus California sea cucumber 1 0.007 Mopalia muscosa mossy chiton 1 0.001 9 0.012

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 35 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI36 Start Date: September 7, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 1 0.043

Embiotoca jacksoni black perch 1 0.006 Phanerodon furcatus white seaperch 1 0.174 Urobatis halleri round stingray 36 7.360 39 7.583
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.002

Pyromaia tuberculata tuberculate pear crab 1 0.001 Octopus bimaculatus/bimaculoides California two

- s pot octopus 1 0.082 Panulirus interruptus California spiny lobster 6 1.328 11 1.413
Non

- Shellfish Diaulula sandiegensis ring

- spotted dorid 1 0.001 1 0.001

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 36 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Surv ey: HGSFI37 Start Date: September 14, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Porichthys myriaster specklefin midshipman 1 0.209

Urobatis halleri round stingray 53 11.127 Paralabrax nebulifer barred s and bass 1 0.183 55 11.519
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.002

Panulirus interruptus California spiny lobster 4 0.875 Pandalus danae dock shrimp 1 0.001 7 0.878
Non

- Shellfish Diaulula sandi egensis ring

- spotted dorid 2 0.002 2 0.002

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 37 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI38 Start Date: September 21, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish G ibbonsia elegans spotted kelpfish 1 0.002

Urobatis halleri round stingray 31 6.234 32 6.236
Shellfish Heptacarpus palpator intertidal coastal shrimp 5 0.002

Pyromaia tuberculata tuberculate pear crab 1 0.001 Panulirus interruptus Cal ifornia spiny lobster 3 0.465 9 0.468
Non

- Shellfish Hermissenda crassicornis hermissenda 1 0.001 1 0.001

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 38 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI39 Start Date: September 28, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 2 0.142

Gibbonsia elegans spotted kelpfish 1 0.022 Heterostichus rostratus giant kelpfish 1 0.013 Urobatis halleri round stingray 50 9.400 Rhacochilus vacca pile perch 1 0.287 Paralabrax nebulifer barred sand bass 2 0.040 57 9.904
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.001

Lysmata californica red rock shrimp 1 0.001 Panulirus interruptus California spiny lobster 8 1.817 12 1.819

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 39 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI40 Start Date: October 5, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Gibbonsia elega ns spotted kelpfish 2 0.010

Urobatis halleri round stingray 52 9.482 54 9.492
Shellfish Portunus xantusii Xantus swimming crab 1 0.043

Heptacarpus palpator intertidal coastal shrimp 3 0.002 Cancer anthonyi yellow crab 1 0.039 Panul irus interruptus California spiny lobster 1 0.180 6 0.264

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -40 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI41 Start Date: October 12, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.119

Gibbonsia elegans spotted kelpfish 1 0.008 Urobatis halleri round stingray 73 13.478 Paralabrax nebulifer barred sand bass 1 0.018 76 13.623
Shellfish Heptacarpus palpator intertidal coastal shrimp 2 0.001

Pyromaia tuberculata tuberculate pear crab 1 0.001 Panulirus interruptus California spiny lobster 2 0.439 5 0.441

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -41 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI42 Start Date: Octo ber 19, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Heterostichus rostratus giant kelpfish 1 0.055

Syngnathus leptorhynchus bay pipefish 1 0.003 Urobatis halleri round stingray 65 12.647 Paralabrax nebulife r barred sand bass 1 0.014 68 12.719
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.002

Pyromaia tuberculata tuberculate pear crab 1 0.001 Panulirus interruptus California spiny lobster 2 0.322 6 0.325
Non

- Shel lfish Hermissenda crassicornis hermissenda 1 0.001

Gastropoda gastropod, unid. 1 0.007 2 0.008

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -42 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI43 Start Date: October 26, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.211

Paralichthys californicus California halibut 1 0.145 Urobatis halleri round stingray 31 5.989 Rhacochilus vacca pile perch 1 0.178 Paralabrax nebulifer barred sand bass 1 0.009 35 6.532
Shellfish Pyromaia tuberculata tuberculate pear crab 2 0.004

Panulirus interruptus California spiny lobster 2 0.712 4 0.716

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -43 Harbor Generating Station

- Normal Operation Impingement Data

- Fish S urvey: HGSFI44 Start Date: November 2, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Gobiesox rhessodon California clingfish 1 0.001

Urobatis halleri round stingray 89 16.259 Acanthogobius flavimanus yellow fin goby 2 0.071 Paralabrax nebulifer barred sand bass 3 0.099 95 16.430
Shellfish Heptacarpus palpator intertidal coastal shrimp 5 0.002

Pyromaia tuberculata tuberculate pear crab 3 0.009 Cancer antennarius Pacific rock crab 1 0.098 Panulirus interruptus California spiny lobster 4 0.949 13 1.058

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -44 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI45 Start Date: November 9, 2006 Survey Totals

Taxon Common Name Abundance Biom ass (kg) Fish Heterostichus rostratus giant kelpfish 1 0.001

Paralichthys californicus California halibut 1 0.680 Urobatis halleri round stingray 18 3.018 Paralabrax nebulifer barred sand bass 1 0.078 21 3.777
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.003 3 0.003

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -45 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI46 Start Date: November 16, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Paralichthys californicus California halibut 1 0.060

Urobatis halleri round stingray 28 5.858 29 5.918
Shellfish Heptacarpus palpator intertidal coastal shrimp 10 0.005

Pyromaia tuberculata tuberculate pear crab 1 0.001 11 0.006

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -46 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI47 Start Date: November 21, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.187

Urobatis halleri round stingray 28 6.199 Paralabrax nebulifer barred sand bass 1 0.038 30 6.424
Shellfish Octopus bimaculatus/bimaculoides California two

- spot octopus 1 0.186 1 0.186

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -47 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI48 Start Date: November 30, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.017

Urobatis halleri round stingray 42 10.070 43 10.087
Shellfish Portunus xantusii Xantus swimming crab 1 0.008

Heptacarpus palpator intertidal coastal shrimp 4 0.002 Pyromaia tuberculata tuberculate pear crab 3 0.005 Panulirus interruptus California spiny lobster 4 0.732 12 0.747

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -48 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI49 Start Date: December 7, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Cymatogaster aggregata shiner perch 1 0.008

Embiotoca jacksoni black perch 2 0.208 Urobatis halleri round stingray 8 1.770 11 1.986
Shellfish Heptacarpus palpator intertidal coastal shrimp 3 0.001 3 0.001

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D -49 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI50 Start Date: December 14, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 1 0.024

Gillichthys mirabilis longjaw mudsucker 1 0.001 Urobatis halleri round stingray 12 2.588 Paralabrax nebulifer barred sand bass 1 0.008 15 2.621
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001

Pyromaia tuberculata tuberculate pear crab 2 0.004 Panulirus interruptus California spiny lobster 2 0.473 5 0.478

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 50 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI51 Start Date: December 21, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 2 0.362

Urobatis halleri round stingray 16 3.085 Paralabrax maculatofasciatus spotted sand bass 1 0.022 19 3.469
Shellfish Heptacarpus palpator intertidal coastal shrimp 5 0.002

Panulirus interruptus California spiny lobster 1 0.333 6 0.335

### Harbor Generating Station

IM&E Final Report Appendix D: Impingement Data

D - 51 Harbor Generating Station

- Normal Operation Impingement Data

- Fish Survey: HGSFI52 Start Date: December 28, 2006 Survey Totals

Taxon Common Name Abundance Biomass (kg) Fish Embiotoca jacksoni black perch 2 0.129

Urobatis halleri round stingray 12 1.992 Tridentiger trigonocephalus chameleon goby 1 0.003 15 2.124
Shellfish Heptacarpus palpator intertidal coastal shrimp 1 0.001 1 0.001

# Harbor Generating Station



# Appendix E Master Species List

E1. Entrainment Master Species List E2. Impingement Master Species List

– Invertebrates E3. Impingement Master Species List
- Fishes

### Harbor Generating Station

IM&E Final Report Appendix E1 : Entrainment Species List

E1 - 1 

# Appendix E1 Master Species List of organisms identified in the

entrainment samples.

Group Taxon Common Name Cephalopoda Loligo opalescens market squid Decapoda unidentified crab (megalops) unid. crab megalops Palinuridae Panulirus interruptus (phyllosome) California spiny lobster (larval) Paguridae Paguridae unid . (megalops) hermit crab megalops Porcellanidae Pachycheles pubescens (megalops) pubescent porcelain crab megalops

Pachycheles rudis (megalops) thickclaw porcelain crab megalops Petrolisthes cinctipes (megalops) flat porcelain crab megalops Petrolis thes eriomerus (megalops) flattop crab megalops Petrolisthes spp. (megalops) porcelain crab megalops Porcellanidae unid. (megalops) porcelain crab megalops Diogenidae Diogenidae (megalops) left

- handed hermit crabs megalops Brachyura Brachyura unid. ( megalops) unidentified crab megalops

Grapsidae / Cancridae (megalops) unidentified crab megalops Majidae Majidae unid. (megalops) spider crab megalops

Pugettia spp. (megalops) kelp crabs megalops Cancridae Cancer antennarius (megalops) brown rock crab megalops

Cancer anthonyi (megalops) yellow crab megalops Cancer gracilis (megalops) slender crab megalops Cancer productus (megalops) red rock crab megalops Cancer spp. (megalops) cancer crabs megalops Xanthidae Lophopanopeus spp. (megalops) black

- clawed crab megalops Pinnotheridae Pinnixa spp. (megalops) pea crabs megalops

Pinnotheres spp. (megalops) pea crab megalops Pinnotheridae (megalops) pea crab megalops Grapsidae Grapsidae unid. (megalops) shore crab megalops

Hemigrapsus nudus (me galops) purple shore crab megalops Hemigrapsus oregonensis (megalops) yellow shore crab megalops Hemigrapsus spp. (megalops) shore crab megalops Pachygrapsus crassipes (megalops) striped shore crab megalops Actinopterygii fish eggs unid. unidentifie d fish eggs

larvae, unidentified yolksac unidentified yolksac larvae larval fish

- damaged unidentified larval fishes larval fish fragment unidentified larval fishes larval/post

- larval fish unid. larval fishes unidentified fish, damaged unidentif ied damaged fish Acanthopterygii Sciaenidae / Paralichthyidae / Labridae (eggs) fish eggs (table continued)

### Harbor Generating Station

IM&E Final Report Appendix E1 : Entrainment Species List

E1 - 2 Appendix E1 (continued). Master Species List of organisms identified in the entrainment samples. Group Taxon Common Name Clupeidae Clupeidae u nid. herrings

Sardinops sagax Pacific sardine Engraulidae Engraulidae unid. anchovies

Engraulidae unid. (eggs) anchovy eggs Engraulis mordax northern anchovy Bathylagidae Bathylagus ochotensis popeye blacksmelt

Leuroglossus spp. smoothtongues Leuroglossus stilbius California smoothtongue Myctophidae Diaphus theta California headlight fish

Myctophidae unid. lanternfishes Stenobrachius leucopsarus northern lampfish Triphoturus mexicanus Mexican lampfish Gobiesocidae Gobiesocidae unid. clin gfishes Gobiesocidae Gobiesox spp. clingfishes Merlucciidae Merluccius productus Pacific hake Atherinopsidae Atherinops affinis topsmelt

Atherinopsidae unid. silversides Atherinopsidae unid. (eggs) silverside eggs Atherinopsis californiensis jacks melt Leuresthes tenuis California grunion Syngnathidae Syngnathus leptorhynchus bay pipefish

Syngnathus spp. pipefishes Scorpaenidae Sebastes spp. rockfishes Hexagrammidae Hexagrammidae unid. greenlings

Oxylebius pictus painted greenling Zaniole pis frenata shortspine combfish Zaniolepis spp. combfishes Artedius harringtoni scalyhead sculpin Cottidae Artedius lateralis smoothhead sculpin

Chitonotus / Icelinus sculpins Chitonotus pugetensis roughback sculpin Clinocottus analis wooly scul pin Cottidae unid. sculpins Icelinus quadriseriatus yellowchin sculpin Icelinus spp. sculpins Leptocottus armatus Pacific staghorn sculpin Oligocottus / Clinocottus sculpins Oligocottus spp. sculpins Orthonopias triacis snubnose sculpin Rus carius creaseri roughcheek sculpin Scorpaenichthys marmoratus cabezon Scorpaenidae Sebastes spp. rockfishes (table continued)

### Harbor Generating Station

IM&E Final Report Appendix E1 : Entrainment Species List

E1 - 3 Appendix E1 (continued). Master Species List of organisms identified in the entrainment samples. Group Taxon Common Name S erranidae Paralabrax spp. sand bass Sciaenidae Cheilotrema saturnum black croaker

Genyonemus lineatus white croaker Genyonemus lineatus (eggs) white croaker eggs Menticirrhus undulatus California corbina Roncador stearnsii spotfin croaker Sciaenidae unid. croakers Sciaenidae unid. (eggs) croaker eggs Seriphus politus queenfish Umbrina roncador yellowfin croaker Kyphosidae Girella nigricans opaleye Pomacentridae Hypsypops rubicundus garibaldi

Pomacentridae unid. damselfishes Sphyraenida e Sphyraena argentea Pacific barracuda Labridae Halichoeres semicinctus rock wrasse

Labridae unid. wrasses Oxyjulis californica senorita Semicossyphus pulcher California sheephead Bathymasteridae Bathymasteridae unid. ronquils Blenniidae Hypsoblen nius gilberti rockpool blenny

Hypsoblennius jenkinsi mussel blenny Hypsoblennius spp. combtooth blennies Clinidae Gibbonsia spp. clinid kelpfishes

Paraclinus spp. clinid Chaenopsidae Chaenopsidae unid. tube blennies Stichaeidae Stichaeidae unid. p ricklebacks Labrisomidae Labrisomidae unid. labrisomid blennies Gobiidae Acanthogobius flavimanus yellowfin goby

Gillichthys mirabilis longjaw mudsucker Gobiidae unid. gobies Ilypnus gilberti cheekspot goby Lepidogobius lepidus bay goby Lythryp nus spp. gobies Lythrypnus zebra zebra goby Rhinogobiops nicholsii blackeye goby Typhlogobius californiensis blind goby Stromateidae Peprilus simillimus Pacific butterfish Pleuronectiformes Pleuronectiformes unid. flatfishes Pleuronectidae Isopsetta isolepis butter sole

Lyopsetta exilis slender sole Parophrys vetulus English sole Pleuronectidae unid. righteye flounders (table continued)

### Harbor Generating Station

IM&E Final Report Appendix E1 : Entrainment Species List

E1 -4 Appendix E1 (continued). Master Species List of organisms identified in the entrainment samples. Group Taxon Common Name Pleuronectidae Pleuronichthys guttulatus diamond turbot

Pleuronichthys ritteri spotted turbot Pleuronichthys spp. turbots Pleuronichthys spp. (eggs) turbot eggs Pleuronichthys verticalis hornyhead turbot Paralichthyidae Citharichthys sordidus Pacific sanddab

Citharichthys spp. sanddabs Citharichthys spp. (eggs) sanddab eggs Citharichthys stigmaeus speckled sanddab Paralichthyidae unid. sand flounders Paralichthyidae unid. (eggs) sand flounder eggs Paralichthys californicus California halibut Paralichthys californicus (eggs) California halibut eggs Xystreurys liolepis fantail sole

### Harbor Generating Station

IM&E Draft Report Appendix E2: Impingement Species List - Invertebrates

E2 - 1 

# Appendix E2 Master Species List of Invertebrates Identified in

Impingement Samples
Group Taxon Common Name Cnidaria Cnidaria sea jelly, unid. Polyorchidae Scrippsia pacifica giant bell jelly Ulmaridae Aurelia aurita moon jelly Ctenophora Ctenonophora comb jelly, unid. Gastropoda Gastropoda gastropod, unid. Aplysiidae Aplysia californica California seahare

Aplysia spp. seahare, u nid. Aglajidae Navanax inermis California aglaja Pleurobranchidae Pleurobranchaea californica California sea slug Archidorididae Archidoris montereyensis Monterey sea

- lemon Discodorididae Anisodoris nobilis Pacific sea

- lemon

Diaulula sandiegensis rin g - spotted dorid Discodorididae nudibranch, unid. Polyceratidae Triopha maculata spotted triopha Polyceridae Polycera atra orange - spike polycera

Polycera hedgpethi Hedgpeth's polycera Dendronotidae Dendronotus frondosus leafy dendronotid

Dendrono tus iris giant

- frond

- aeolis Dironidae Dirona picta spotted dirona Flabellinidae Flabellina trilineata threeline aeolis Tergipedidae Cuthona lagunae orange - faced nudibranch Facelinidae Hermissenda crassicornis hermissenda Pectindae Leptopecten spp. scallop, unid. Octopodidae Octopus bimaculatus/bimaculoides California two

- spot octopus Pycnogonida Pycnogonida sea spider, unid. Ammotheaidae Ammothea hilgendorfi sea spider Penaeidae Farfantepenaeus californiensis yellowleg shrimp Pandalidae Pandalus d anae dock shrimp Pandalus stenolepis roughpatch shrimp Hippolytidae Heptacarpus palpator intertidal coastal shrimp

Heptacarpus paludicola California coastal shrimp Heptacarpus spp. coastal shrimp, unid. Heptacarpus stimpsoni Stimpson coastal shrimp Lysmata californica red rock shrimp Crangonidae Crangon nigromaculata blackspotted bay shrimp Palinuridae Panulirus interruptus California spiny lobster Majidae Pyromaia tuberculata tuberculate pear crab (table continued)

### Harbor Generating Station

IM&E Draft Report Appendix E2: Impingement Species List - Invertebrates

E2 - 2 Appendix E1 (continu ed). Master Species List of invertebrates identified in the impingement samples. Group Taxon Common Name Cancridae Cancer antennarius Pacific rock crab

Cancer anthonyi yellow crab Portunidae Portunus xantusii Xantus swimming crab Grapsidae Hemigrapsus oregonensis yellow shore crab Pinnotheridae Pinnixa spp. pea crab, unid. Asteriidae Pisaster spp. sea star, unid. Holothuroidea Holothuroidea sea cucumber, unid. Stichipodidae Parastichopus californicus California sea cucumber

Parastichopus parvime nsis warty sea cucumber Parastichopus spp. sea cucumber, unid. Molpadiidae Molpadia muscosa mossy chiton

### Harbor Generating Station

IM&E Draft Report Appendix E3: Impingement Species List - Fishes

E3 - 1 

# Appendix E3 Master Species List of fishes identified in the impingement samples.
Group Taxon Common Name (Chondrichthyes) cartilaginous fishe s Urolophidae Urobatis halleri round stingray (Actinopeterygii) ray - finned fishes Engraulidae Engraulis mordax northern anchovy Batrachoididae Porichthys myriaster specklefin midshipman Porichthys notatus plainfin midshipman Atherinopsidae At herinops affinis topsmelt Syngnathidae Syngnathus leptorhynchus bay pipefish Cottidae Icelinus quadriseriatus yellowchin sculpin Leptocottus armatus Pacific staghorn sculpin Serranidae Paralabrax maculatofasciatus spotted sand bass Paralabrax nebul ifer barred sand bass Sciaenidae Genyonemus lineatus white croaker Embiotocidae Cymatogaster aggregata shiner perch Embiotoca jacksoni black perch Embiotocidae surfperch, unid. Hyperprosopon argenteum walleye surfperch Phanerodon furcatus white seaperch Rhacochilus vacca pile perch Clinidae Gibbonsia elegans spotted kelpfish Heterostichus rostratus giant kelpfish Gobiesocidae Gobiesox rhessodon California clingfish Gobiidae Acanthogobius flavimanus yellowfin goby Gillichthys mirabilis longjaw mudsucker Tridentiger trigonocephalus chameleon goby Paralichthyidae Paralichthys californicus California halibut Pleuronectidae Pleuronichthys verticalis hornyhead turbot