PROGRESS REPORT: SEPTEMBER 2010

BIODEGRADATION TESTING SUPPLEMEN TAL ENVIRONMENTAL PROJECT

Submitted to

E.I du Pont de Nemours and Company

Wilmington, Delaware 19898

U.S.A.

United States Environmental Protection Agency

Washington, DC

U.S.A.



# Wildlife International, Ltd.

8598 Commerce Drive

Easton, Maryland 21601 USA

1 (410) 822-8600

September 29, 2010

Page 1 of 5Does Not Contain Confidential Business Information (CBI)

2010_September_Progress Report 2 Table of Contents

Title Page ..................................................................................................................... ..............1

Table of Contents.............................................................................................................. .........2

Introduction................................................................................................................... .............3

Progress Summary ............................................................................................................... ......3

Appendix A Invoice............................................................................................................. ......4Does Not Contain Confidential Business Information (CBI)

2010_September_Progress Report 3 Introduction

This document presents the pr ogress of the technical work for the Biodegradation Testing

Supplemental Environmental Project (“Biode gradation SEP”) performed from 25 August

2010 up to 24 September 2010. Studi es conducted under protocol will be examined by the

Wildlife International Quality Assurance Unit for compliance with Good Laboratory

Practices, Standard Operating Procedures and the specified protocol. Ra w data that has been

audited by the Wildlife International Quality Assurance will be included in the progress

report, as appropriate.

Progress Summary

The total costs associated with the project for the period covered in this Progress Report are

approximately $116,578 for labor and $18,900 for in strumentation. The invoice and an

allocation of costs with associated tasks are presented in Appendix A.

The technical work consisted of analytical proc essing of samples from th e initial pi lot study.

Bench processing and determination of study an alytes by GC/MS and LC/MS/MS has been

completed for all pilot study samples.

Estimated completion dates for un-audited an d audited draft report are 22 October and 17

November, respectively.Does Not Contain Confidential Business Information (CBI)

2010_September_Progress Report 4 Appendix A

InvoiceDoes Not Contain Confidential Business Information (CBI)2010_September_Progress Report5Allocation of CostsMonth, Year Tasks TotalDesign andProcurement ofSCAS Units(Task A)Acquisitionof Standards(Task B)Adaptation ofAnalyticalMethod (Task C)Pilot StudiesTask (D)Refinement ofMethodsTask (E)MethodValidations (Task F)StorageStability(Task G)BiodegradationStudies(Task H)InstrumentLeaseDec 2009 $1,803 $1,085 $42,422 $45,310Jan 2010 $29,227 $29,227Feb 2010 $1,300 $36,745 $38,045Mar 2010 $14,350 $14,350 $18,900Apr 2010 $570 $15,935 $16,505 $18,900May 2010 $760 $71,860 $72,620 $18,900June 2010 $57,870 $35,003 $92,873 $18,900July 2010 $42,430 $15,130 $57,560 $18,900August 2010 $32,530 $113,718 $146,248 $18,900September 2010 $1,350 $115,228 $116,578 $18,900Labor Total $1,803 $3,715 $344,719 $279,078 $629,315Materials $6,800 $10,500 $20,644 $1,724 $39,669Total Cost $8,603 $14,215 $365,363 $280,802 $668,983 $132,300