# Update of Human Health Ambient Water Quality Criteria: gamma - Hexachlorocyclohexane (HCH) 58

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# Update of Human Health Ambient Water Quality Criteria: gamma - Hexachlorocyclohexane (HCH) 58

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Office of Science and Technology Office of Water U.S. Environmental Protection Agency Washington, DC 2046 0Gamma

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Table of Contents
1 Introduction: Background and Scope of Update

..................................................................... 2 2 Problem Formulation

............................................................................................................... 3 3 Criteria Formulas: Analysis Plan

.............................................................................................. 4 4 Exposure Factors

..................................................................................................................... 5 4.1 Body Weight

.................................................................................................................. 5 4.2 Drinking W ater Intake

................................................................................................... 6 4.3 Fish Consumption Rate

................................................................................................ . 6 4.4 Bioaccumulation Factor

................................................................................................ 7 4.4.1 Approach

......................................................................................................... 7 4.4.2 Chemical

- specific BAFs

.................................................................................... 9 5 Hazard Identification and Dose Response

............................................................................. 10 5.1 Approach

..................................................................................................................... 10 5.2 Chemical

- spe cific Toxicity Value

................................................................................. 11 5.2.1 Reference Dose

.............................................................................................. 11 5.2.2 Cancer Slope Factor

....................................................................................... 12 6 Relative Source Contribution

................................................................................................ . 12 6.1 Approach

..................................................................................................................... 12 6.2 Chemical

- specific RSC

................................................................................................ . 13 7 Criteria Derivation: Analysis

.................................................................................................. 15 7.1 AWQC for Noncarcinogenic Toxicological Effects

...................................................... 16 7.2 AWQC for Carcinogenic Toxicological Effects

............................................................. 16 7.3 AWQC Summary

......................................................................................................... 16 8 Criteria Characterization

........................................................................................................ 17 9 Chemical Name and Synonyms

............................................................................................. 19 10 References

............................................................................................................................. 22

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1 Introduction: Background and Scope of Update EPA’s recommended ambient water quality criteria (AWQC) for human health are scientifically derived numeric values that EPA has determined will adequately protect human health from the adverse effects of pollutants in ambient water. Section 304(a)(1) of the Clean Water Act (CWA) requires EPA to develop and publish, and from time to time revise, recommended criteria for the protection of water quality that accurately reflect the latest scientific knowledge. Water quality criteria developed under section 304(a) are based solely on data and scientific judgments on the relationship between pollutant concentrations and environmental and human healt h effects. Section 304(a) criteria do not reflect consideration of economic impacts or the technological feasibility of meeting pollutant concentrations in ambient water. EPA’s recommended section 304(a) criteria provide technical information for states an d authorized tribes a to consider and use in adopting water quality standards that ultimately provide the basis for assessing water body health and controlling discharges of pollutants into waters of the United States. Under the CWA and its implementing reg ulations, states and authorized tribes are required to adopt water quality criteria to protect the designated uses of waters (e.g., public water supply, aquatic life, recreational use, industrial use). EPA’s recommended water quality criteria do not substi tute for the CWA or regulations, nor are they regulations themselves. Thus, EPA’s recommended criteria do not impose legally binding requirements. States and authorized tribes may adopt, where appropriate, other scientifically defensible water quality crit eria that differ from these recommendations. The water quality criteria that are the subject of this document are national AWQC recommendations for human health issued under CWA section 304(a). Unless expressly indicated otherwise, all references to “criteria,” “water quality criteria,” “ambient water quality criteria recommendations,” or similar variants thereof are references to national AWQC recommendations for human health. In this 2015 update, EPA has revised the human health criteria for gamma h exachl orocyclohexane (HCH) to reflect the latest scientific information, including updated exposure factors (body weight [BW], drinking water intake [DI] rate, and fish consumption rate [FCR]), bioaccumulation factors (BAFs), and human health toxicity value s ( reference dose [RfD] multiplied by relative source contribution [RSC] or 10 -6 divided by cancer slope factor [CSF]). Th e criteria continue to be based on EPA’s Methodology for Deriving Ambient Water Quality Criteria for the Protection of Human Health , whic h is referred to as the “2000 Methodology” in this document (USEPA 2000a) . EPA accepted written scientific views from the public on the draft updated human health criteria for this chemical (and 93 others) from May t hrough August 2014.

a Throughout this document, the term states means the 50 states, the District of Columbia, the Commonwealth of Puerto Rico, the Virgin Islands, Guam, American Samoa, and the Commonwealth of the Northern Mariana Islands. The term authorized tribe or tribe means an Indian tribe authorized for treatment in a manne r similar to a state under CWA s ection 518 for the purposes of s ection 303(c) water quality standards

.

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I t is important for s tates and authorized t ribes to con sider any new or updated section 304(a) recommended criteria as part of their triennial review process to ensure that state or tribal water quality standards reflect current science and protect applicable designated uses. These final 2015 updated section 3 04(a) human health criteria recommendations supersede EPA’s previous recommendations.
2 Problem Formulation Problem formulation provides a strategic framework for water quality criteria development by focusing on the most relevant endpoints and increasing th e transparency of the effects assessment. The structure of this criteria document is intended to be consistent with general concepts of effects assessments as described in EPA’s Framework for Human Health Risk Assessment to Inform Decision Making (USEPA 2014a)

. In developing AWQC, EPA currently follows the assessment method outlined in its 2000 Methodology (USEPA 2000a)

. The 2000 Methodology describes different approaches for addressing water and non

- water exposure pathways to derive human health AWQC depending on the toxicological endpoint of concern, the toxicological effect (non carcinogenic or carcinogenic ), and whether toxicity is considered a linear or threshold effect. Water sources of exposure include both consuming drinking water and eating fish or shellfish from inland and nearshore waters that have been exposed to pollutants in the water body

. For pollutants that exhibit a threshold of exposure before deleterious effects occur, as is the case for noncarcinogens and nonlinear carcinogens, EPA applies an RSC to account for other potential human exposures to the pollutant (USEPA 2000a)

. Other sources of exposure m ight include, but are not limited to, exposure to a particu lar pollutant from ocean fish or shellfish consumption (which is not included in the FCR ), non

- fish food consumption (e.g., consumption of fruits, vegetables, grains , meats, or poultry ), dermal exposure, and inhalation exposure. For substances for which the toxic ity endpoint is carcinogenicity based on a linear low

- dose extrapolation, only the exposures from drinking water and fish ingestion are reflected in human health AWQC; that is , non - water sources are not explicitly included and no RSC is applied (USEPA 2000a) . In these situations, A WQC are derived with respect to the incremental lifetime
cancer risk posed by the presence of a substance in water, rather than an individual’s total risk from all sources of exposure. The resulting criterion represent s the water concentration that is expe cted to increase an individual’s lifetime risk of ca ncer from exposure to the particular pollutant by no more than one chance in one million for the general population. EPA calculates AWQC at a 10 -6 (one in one million) cancer risk level for the general po pulation (USEPA 2000a)

. The 2000 Me thodology recommends that states set human health criteria cancer risk levels for the target general population at either 10 -5 or 10 - 6 and also notes that states and authorized t ribes can choose a more stringent risk level, such as 10 -7

. For substances tha t are carcinogenic, EPA takes an integrated approach and considers both cancer and n oncancer effects when deriving AWQC (USEPA 2000a; USEPA 2000b)

. Where sufficient data are available, EPA derives AWQC using both c arcinogenic and noncarcinogenic toxicity endpoints and recommends the lower value fo r the AWQC. The AWQC might no t utilize

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the value obtained from the cancer analysis if it is less protective than that derived from the noncancer endpoint

. 3 Criteria Formulas : Analysis Plan Human health AWQC for toxic pollutants are necessary to protect any designated uses related to in gestion of water and ingestion of aquatic organisms. These uses can include, but are not limited to, recreation in and on the water, consumption of fish or shellfish (including consumption associated with fishing or shellfish harvesting), and protecti on of drinking water supplies.
The derivation of human health AWQC requires information about both the toxicological endpoints of concern for water pollutants and the pathways of human exposure to those pollutants. EPA considers the following two primary pathwa ys of human exposure to pollutants present in a particular water body when deriving human health 304(a) AWQC: (1) d irect ingestion of drinking water obtained from the water body and (2) c onsumption of fish or shellfish obtained from the water body. The equ ations for deriving human health AWQC for non carcinogenic effects and carcinogenic effects are presented as Eq s . 1 and 2. EPA d erives recommended human health AWQC based on the consumption of both water and aquatic organisms (Eq

. 1) and based on the consumption of aquatic organisms alone (Eq

. 2). The use of one criterion over the other depends on the designated use of a particular water body or water bodies (i.e., drinking water source and/or fishable waters). EPA recommends applying organism

- only AWQ C (Eq. 2) to a water bod y where the designated use includes supporting fishable uses under section 101(a) of the CWA but the water body is not a drinking water supply source (e.g., non

- potable estuarine waters that support fish or shellfish for human consu mption) (USEPA 2000a)

. EPA recommen ds inclu ding the drinking water exposure pathway for ambient surface waters where drinking water is a designated use for the following reasons: (1) drinking water is a designated use for surface waters under the CWA , and therefore criteria are needed to en sure that this designated use can be protected and maintained; (2) although they are rare, some public water supplies provide drinking water from surface water sources without treatment; (3) even among the majority of water supplies that do treat surface w aters, existing treatments
m ight not be effective for reducing levels of particular contaminants; and (4) in consideration of the Agency’s goals of pollution prevention, ambient waters should not be contaminated to a level where the burden of achieving health objectives is shifted away from those responsible for pollutant discharges and placed on downstream users that must bear the costs of upgraded or supplemental water treatment (USEPA 2000a)

.

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The equations for deriving the criteria values are as follows (USEPA 2000a) : For consumption of water and organisms: AWQC (μg/L) = t oxicity value (mg/kg

- d) × BW (kg) × 1 , 000 ( μg/mg ) b (Eq. 1) DI (L/d) + ∑ (FCR i (kg/d) × BAF i (L/kg)) 4 i=2 For consumption of organisms only: AWQC (μg/L) = toxicity value (mg/kg

- d) × BW (kg) × 1 , 000 ( μg/mg ) c (Eq. 2) ∑ (FCR i (kg/d) × BAF i (L/kg))

4 i=2 Where: AWQC = ambient water quality criteria toxicity value = RfD x RSC ( mg/kg

- d) for non carcinogenic effects
or 10 -6 /CSF ( kg

- d/mg) for carcinogenic effects d RSC = relative source contribution (applicable to only noncarcinogenic and nonlinear low

- dose extrapolation for carcinogenic effects) BW = body weight DI = drinking water intake ∑ 4

i=2 = summation of values for aquatic trophic levels (TLs), where the letter i stand s for the TLs to be considered, starting with TL2 and proceeding to TL4 FCR i = fish consumption rate for aquatic TLs 2, 3, and 4 BAF i = bioaccumulation factor for aquatic TLs 2, 3, and 4 EPA rounds AWQC to the number of significant figures in the least pre cise parameter as described in the 2000 Methodology (USEPA 2000a , s ection 2.7.3).

4 Exposure Factors
4.1 Body Weight EPA updated the default BW assumption to 80 .0 kg based on National Health and Nutrition Examination Survey (NHANES) data from 1999 to 2006 as r eported in Table 8.1 of EPA’s Exposure Factors Handbook (USEPA 2011a) . The updated BW represents the mean weight for adults ages 21 and older. EPA’s previously recommended BW assumption for adults was 70 kg, which was based on the mean BW of adults from the NHANES III database (1988

– 1994) and a 1989 study conducted by the National Cancer Institute (USEPA 2000a)

.

b 1,000 μg/mg is used to convert the units of mass from milligrams to micrograms.

c 1,000 μg/mg is used to convert the units of mass from milligrams to micrograms. d 10 -6 or 1 in 1,000,000 risk level for the general population.

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4.2 Drinking Water Intake
EPA updated the default DI to 2.4 L/d, rounded from 2.414 L/ d, based on NHANES data from 2003 to 2006 as reported in EPA’s Exposure Factors Handbook (USEPA 2011a , Table 3

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. This rate represents the per capita estimate of combined direct and indirect community water e ingestion at the 90 th percentile for adults ages 21 and older

. EPA selected the per capita rate for the u pdated DI because it represents the average daily dose estimates ; that is , it includes both people who drank water during the survey period and those who did not , which is appropriate for a national

- scale assessment such as CWA section 304(a) national human health criteria development (USEPA 2011a, section 3.2.1 ).
EPA’s updated DI o f 2.4 L/ d is consistent with the 2000 Methodology . In that document, EPA recommended a default DI of 2 L/d , which represented the per capita community water ingestion rate at the 86 th percentile for adults surveyed in the U .S. Department of Agriculture’s 1 994

– 1996 Continuing Survey of Food Intake by Individuals (CSFII) analysis (USEPA 2000a , section 4.3.2.1 ).
4.3 Fish Consumption Rate The updated FCR for the general adult population is 22.0 g/d, or 0.0220 kg/d (USEPA 2014b , Table 9a ). This FCR represents the 90 th percentile per capita consumption rate of fish from inland and nearshore waters for U.S. adults ages 21 years and older based on NHANES data from 2003

– 2010. The 95 percent confidence interval (CI) of the 90 th percentile per capita FCR is 19.1 g/d and 25.4 g/d. This updated FCR replaces EPA’s previously recommended default FCR of 17.5 g/d, which represented an estimate of the 90 th percentile per capita consumption rate of fish from inland and nearshore waters for U.S. adults ages 21 years and older. That de fault FCR was based on USDA’s CSFII 1994

– 1996 data (USEPA 2002a)

. As recommended in the 2000 Methodology, EPA updated the AWQC to reflect trophic level - (TL)

- specific FCRs to better represent human dietary consumption of fish. An organism’s trophic position in the aquatic food web can have an important effect on the magnitude of bioaccumulation of certain chemicals. The TL

- specific FCRs are numbered 2, 3, and 4, and they account for different categories of fish and shellfish species based on their position in the aquatic food web: TL2 accounts for benthic filter feeders; TL3 accounts for forage fish; and TL4 accounts for predatory fi sh (USEPA 2000a)

. EPA used the following TL

- specific FCRs to derive the updated AWQC: TL2 = 7.6 g/d (0.0076 kg/d ) (95 percent CI [6.4, 9.1] g/d ); TL3 = 8.6 g/d (0.0086 kg/d) (95 percent CI [7.2, 10.2] g/d ) ; and TL4 = 5 .1 g/d (0.005 1 kg/d) (95 percent CI [4.0, 6.4] g/d )

. Each TL

- specific FCR represent s the 90 th percentile per capita consumption rate of fish and shellfish from inland and nearshore waters from that particular TL for U.S. adult s ages 21 years and older (USEPA 2014b ,

e Community water includes direct and indirect use of tap water for household uses and excludes bottled water and other sources (USEPA 2011a, section 3.3.1.2 ). Direct ingestion is defined as direct consumption of water as a beverage, while indirect ingestion includes wa ter added during food preparation (e.g., cooking, rehydration of beverages) but not water intrinsic to purchased foods (USEPA 2011a, section 3.1 ).

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Tables 16a, 17a, and 18a ) . The sum of these three TL

- specific FCRs is 21.3 g/d, which is within the 95 per cent CI of the overall FCR of 22.0 g/d. EPA recommends using the TL

- specific FCRs when deriving AWQC; however, the overall FCR rate (22.0 g/d) may be used if a simplified approach is preferred.
4.4 Bioaccumulation Factor
4.4.1 Approach Several attributes of the bioa ccumulation process are important to understand when deriving national BAFs for use in developing national recommended section 304(a) AWQC. First, the term bioaccumulation refers to the uptake and retention of a chemical by an aquatic organism from all surrounding media, such as water, food, and sediment. The term bioconcentration refers to the uptake and retention of a chemical by an aquatic organism from water only. For some chemicals (particularly those that are highly persistent and hydrophobic), the ma gnitude of bioaccumulation by aquatic organisms can be substantially greater than the magnitude of bioconcentration. Thus, an assessment of bioconcentration alone might underestimate the extent of accumulation in aquatic biota for those chemicals. Accordingly, the EPA guidelines presented in the 2000 Methodology emphasize using, when possible, measured or estimated BAFs, which account for chemical accumulation in aquatic organisms from all potential exposure routes (USEPA 2000a)

. EPA estimated BAFs for this updated AWQC using EPA’s 2000 Meth odology (USEPA 2000a) and its Techn ical Support Document, Volume 2: Development of National Bioaccumulation Factors (Technical Support Document, Volume 2) (USEPA 2003a) . Specifically, these documents provide a framework for identifying alternative procedures to der ive national trophic level

- specific BAFs for a chemical based on the chemical’s properties (e.g., ionization and hydrophobicity), metabolism, and biomagnification potential (USEPA 2000a; USEPA 2003a)

. EPA’s approach for developing national BAFs represents the long

- term average bioaccumulation potential of a pollutant in aquatic organisms that are commonly consumed by humans across the United States. National BAFs are not intended to reflect fluctuations in bioaccumulation over short periods (e.g., a few days) because human health AWQC are generally designed to protect humans from long

- term (lifetime) exposures to waterborne chemicals (USEPA 2003a)

. EPA followed the approach desc ribed in Figure 3

- 1 of the Technical Support Document, Volume 2 (USEPA 2003a) . EPA used peer

- reviewed, publicly available information to classify each chemical using this framework to derive the most appropriate BAFs according to EPA’s 2000 Methodology (USEPA 2000a) . The framework provides six alternatives, or procedures, resulting in up to four possible methods for each chemical, based on the chemical’s properties. These four methods follow:

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• BAF Method. This method uses measured BAFs derived from data obtained from field studies. Field

- measured BAFs were normalized by adjusting for the water

- dissolved portions of the chemical and the lipid fraction of fish tissue for each species, as well as the fraction of the total concentration of chemical in water that is freely dissolved. EPA averaged multiple field BAFs using a geometric mean of the normalized BAFs by species and trophic level; then EPA further averaged the BAFs across species to compute TL baseline BAFs. The national

- level BAF adjusts the TL baseline BAFs by n ational default values for lipid content, dissolved and particulate organic carbon content, and the n

octanol

- water partition coefficient (K ow ). EPA chose the recommended 50 th percentile dissolved and particulate organic carbon content for the national

- lev el default values, as described in section 6.3 of the Technical Support Document, Volume 2 (USEPA 2003a)

. • BSAF Method. This method uses biota

- sediment accumulation factors (BSAFs) to estimate BAFs. EPA did not use measured BSAFs to calculate national BAFs because the two major compilations of these data

— EPA’s Biota

- Sediment Accumulation Factor Data Set, Version 1.0 (USEPA 2015a) , and the U.S. Army Corps of Engineers’ BSAF database (USACE 2015)

— have not been peer

- reviewed.

• BCF Method. This method uses BAFs estimated from laboratory

- measured bioconcentration factors (BCFs) with or without adjustment by a food chain multiplier. Similar to field BAFs, laboratory

- measured BCFs are normalized with the lipid fraction and the fraction of the total concentration of chemical in water that is freely dissolved, then multiplied by the food chain multiplier where applicable. Multiple values are averaged using a geometric mean across species and then across trophic level to compute baseline BAFs. The national

- level BAF adjusts the TL baseline BAFs by national default values for lipid content, dissolved and particulate organic carbon content, and the K ow . EPA chose the recommended 50 th percentile dissolved and particulate organic carbon content for the national

- level default values, as described in section 6.3 of the Technical Support Document, Volume 2 (USEPA 2003a)

. • K ow Method. This me thod predicts BAFs based on a chemical’s K ow , with or without adjustment using a food chain multiplier, as described in section 5.4 of the Technical Support Document, Volume 2 (USEPA 2003a)

. Following the decision framework presen ted in Figure 3

- 1 of the Technical Support Document, Volume 2 (USEPA 2003a) , EPA selected one of the six procedures to develop a national

- level BAF for this chemical. For a given procedure, EPA selected the method that provided BA F estimates for all three trophic levels (TL2

– TL4) in the following priority:
1. BAF estimates using the BAF method (i.e., based on field

- measured BAFs) if possible. 2. BAF estimates using the BCF method if (a) the BAF method did not produce estimates for all th ree trophic levels and (b) the BCF method produced national

- level BAF estimates for all three trophic levels. 3. BAF estimates using the K ow method if (a) Procedure 1 or 3 was applicable (see Figure 3- 1 of the Technical Support Document, Volume 2 [ USEPA 2003a ] ) and (b) the BAF and BCF methods did not produce BAF estimates for all three trophic levels.

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In cases where the procedure called for the BAF method but there were fewer than three trophic le vel estimates and the K ow method did not apply (i.e., Procedures 2, 4, 5, and 6), EPA used the BAF method estimate for the reported trophic levels by averaging the estimates using a geometric mean when there were two BAFs and us ing the single estimate when only one was available. EPA did not mix values from the BAF and BCF methods. If the BAF method did not have sufficient reliable data for any trophic levels, EPA used the BCF method estimates in the same manner. If none of the four methods provided suffici ent data, or if none were appropriate for the procedure, EPA used the BCF from the previously recommended 2002/2003 criteria (USEPA 2002b; USEPA 2003b)

. EPA primarily used field

- measured BAFs an d laboratory

- measured BCFs available from peer

reviewed, publicly available databases (Arnot and Gobas 2006; Environment Canada 2006) to develop national BAFs. If field

- measured BAFs and laboratory

- measured BCFs were not available from those sources, EPA selected K ow values from peer

- reviewed sources (i.e., Agency for Toxic Substances and Disease Registry [ATSDR] preferentially, followe d by U.S. Department of Health and Human Services ’ Hazardous Substances Data Bank ) for use in calculating national BAFs using the K ow method described in EPA’s Technical Support Document, Volume 2 (USEPA 2003a) . For those chemical s for which the K ow method was not applicable based on the Technical Support Document, Volume 2 (USEPA 2003a) , EPA performed open literature searches of peer

- reviewed journal articles to find field

- measured BAFs or laboratory

- meas ured BCFs.
4.4.2 Chemical

- specific BAFs EPA selected national BAF values of 1,200 L/kg (TL2), 2,400 L/kg (TL3), and 2,500 L/kg (TL4) for gamma - HCH

. EPA followed the framework for selection of methods for deriving national BAFs in Figure 3

- 1 of the Technical Supp ort Document, Volume 2 (USEPA 2003a) to select a procedure for estimating national BAFs for gamma

- HCH . Based on the characteristics of this chemical, EPA selected Procedure 3 for deriving a national BAF value. Gamma

- HCH has the following characteristics:

• Nonionic organic chemical (USDHHS 2010)

• Low hydrophobicity (log K ow < 4); log K ow = 3.72 (ATSDR 2005)

• Low/unknown metabolism
EPA was able to locate peer

- reviewed, field

- measured BAFs for TLs 2, 3, and 4 (Arnot and Gobas 2006; Environment Canada 2006) . Therefore, EPA used the Field BAF method to derive the national BAF values for this chemical:
TL 2 = 1,200 L/kg TL3 = 2,400 L/kg TL4 = 2,500 L/kg

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5 Hazard Identification and Dose Response
5.1 Approach EPA considered all available toxicity values for both noncarcinogenic and carcinogenic toxicological effects to develop this updated AWQC for gamma - HCH . As described in the 2000 Methodology (USEPA 2000a) , where data are available EPA derives AWQC for both noncarcinogenic and carcinogenic effects and recommends the more protective value for the AWQC. (S ee section 7, Criteria Derivation: Analysis .) For noncarcinogenic toxicological effects, EPA uses a chronic

- duration oral RfD to derive human health AWQC. An RfD is an estimate (with uncertainty spa nning perhaps an order of magnitude) of a daily oral exposure of the human population to a substance that is likely to be without an appreciable risk of deleterious effects during a lifetime. An RfD is typically derived from a laboratory animal dosing stud y in which a no

- observed

- adverse

- effect level (NOAEL), lowest

observed

- adverse

- effect level (LOAEL), or benchmark dose can be obtained. Uncertainty factors are applied to reflect the limitations of the data (USEPA 2000a)

. For carcinogenic toxicological effects, EPA uses an oral CSF to deriv e human health AWQC . The oral CSF is an upper bound, approximating a 95 percent confidence limit, on the increased cancer risk from a lifetime oral exposure to a stressor. For this update, EPA conducted a systematic search of eight peer - reviewed, publicly available sources to obtain the toxicity value (RfD or CSF) for use in developing AWQC

. EPA’s primary source of toxicity values for developing human health AWQC is its Integrated Risk Information System (IRIS) program (USEPA 2015b)

. EPA also systematically searched for toxicological assessments from the following EPA program offices, other national and international programs, and state programs :

• EPA , Office of Pesticide Programs (USEPA 2015c)

• EPA, Office of Pollution Prevention and Toxics (USEPA 2015d)

• EPA, Office of Water (USEPA 2015e)

• EPA, Office of Solid Waste and Emergency Response (USEPA 2015f)

• U.S. Department of Health and Human Services , Agency for Toxic Substances and Disease Reg istry (ATSDR 2015)

• Health Canada (HC 2015)

• California Environmental Protection Agency, Office of Environmental Health Hazard Assessment (CalEPA 2014)
After identifying and documenting all available toxicity values, EPA followed a systematic process to select the toxicity value s used to derive the AWQC for noncarcinogenic and carcinogenic effects. EPA selected IRIS toxicity value s to derive the updated AWQC if any of the following conditions were met:

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1. EPA’s IRIS toxicological assessment was the only available source of a toxicity value. 2. E PA’s IRIS toxicological assessment was the most current source of a toxicity value. 3. EPA’s IRIS program was reassessing the chemical in question and had published the draft Toxicological Review for public review and comment, discussion at a public meeting, and subsequent expert peer review

. f 4. The toxicity value from a more current toxicological assessment from a source other than EPA IRIS was based on the same principal study and was numerically the same as
an older EPA IRIS toxicity value

. 5. A more current toxicological assessment from a source other than EPA IRIS was available, but it did not include the relevant toxicity value (chronic

- duration oral RfD or CSF)

. 6. A more current toxicological assessment from a source other than EPA IRIS was available, but it di d not introduce new science (e.g., the toxicity value was not based on a newer principal study) or use a more current modeling approach compared to an older EPA IRIS toxicological assessment.

EPA selected the toxicity value from a peer - reviewed, publicly a vailable source other than EPA IRIS to derive the upd a ted AWQC if any of the following conditions were met:
1. The chemical is currently used as a pesticide , and EPA Office of Pesticide Programs had a toxicity value that was used in pesticide registration decision

- making

. 2. A toxicological assessment from a source other than EPA IRIS was the only available source of a toxicity value. 3. A more current toxicological assessment from a source other than EPA IRIS introduced
n ew science (e.g., the toxicity value was based on a newer principal study) or used a more current modeling approach compared to an older EPA IRIS toxicological assessment.

5.2 Chemical

- specific Toxicity Value
5.2.1 Reference Dose EPA selected an RfD of 4.7 × 10 –3 mg/kg -d (0.0047 mg/kg -d ) for gamma

- HCH based on a 2002 EPA Office of Pesticide Programs (OPP) Reregistration Eligibility De cision (RED) (USEPA 2002c)

. EPA OPP identified a study by Amyes ( 1989) as the critical study and periacinar hepatocyte hypertrophy, increased liver/spleen weight, and decreased platelets as the critical effects in rats orally exposed to gamma

- HCH (USEPA 2002c) . The study had a NOAEL of 0.47 mg/kg -d

. In deriving the RfD, EPA OPP applied a composite uncertainty factor of 100 to account for interspecies extrapolation (10) and intraspecies variation (10) (USEPA 2002c)

.

f Equivalent to Step 4 in the July 2013 EPA Process for Developing IRIS Health Assessments. Available online at http://www.epa.gov/iris/process.htm

.

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EPA identified three other potential RfD sources through the systematic search described in section 5: a 1986 EPA IRIS assessment (USEPA 1986) , a 1999 California EPA assessment (CalEPA 1999) , and a 2005 ATSDR assessment (ATSDR 2005)

. Based on the selection process described in section 5, the 2002 OPP RfD is preferred for use in AW QC development at this time

. The ATSDR assessment does not include the relevant (chronic oral) toxicity endpoint. The OPP RED is the most current available RfD source

. 5.2.2 Cancer Slope Factor Under the 1999 EPA Review Draft Guidelines for Carcinogen Risk Assessment (USEPA 1999) , E PA OPP classified gamma - HCH as “ suggestive evidence of carcinogenicity, but not sufficient to assess human carcinogenic potential ” (USEPA 2001; USEPA 2002c)

. Citing a 2001 C ancer Asse ssment Review Committee report on the carcinogenicity of gamma - HCH (USEPA 2001) , OPP did not quantify human cancer risk in it s 2002 RED (USEPA 2002c)

. EPA’s IRIS program has not developed a carcinogenicity assessment for gamma

- HCH (USEPA 1986)

. E PA identified one sourc e of a CSF for gamma - HCH through the systematic search described in section 5: a 1999 California EPA assessment (CalEPA 1999) . Based on the selection process described in section 5, EPA is not selecting this CSF for AWQC development at this time

. Cancer risk was not quantified by EPA in its most recent assessment (USEPA 2001; USEPA 2002c)

. Thus, EPA will not derive AWQC for carcinogenic toxicological effects for gamma

- HCH at this time.
6 Rela tive Source Contribution
6.1 Approach Th e RSC component of the AWQC calculation allows a percentage of the RfD’s exposure to be attributed to the consumption of ambient water and fish and shellfish from inland and nearshore waters when there are other potentia l exposure sources. The RSC describes the portion of the RfD available for AWQC

- related sources (USEPA 2000a) ; the remainder of the RfD is allocated to other sources of the pollutant. The rationale for this approach is that for pollutants exhibiting threshold effects, the objective of the A WQC is to ensure that an individual’s total exposure from all sources does not exceed that threshold level. Exposures outside the RSC include, but are not limited to, exposure to a particular pollutant from ocean fish and shellfish consumption (which is not included in the FCR ), non

- fish food consumption (e.g., fruits, vegetables, grains, meats, poultry), dermal exposure, and respiratory exposure.
EPA derived an RSC for each chemical included in this 2015 update by using the Exposure Decision Tree approach described in the 2000 Methodology (USEPA 2000a)

. To u s e th at approach, EPA compiled information for each chemical on its uses, chemical and physical properties, occurrences in other potential sources (e.g., air, food), and releases to the environmen t, as well as regulatory restrictions on other sources that are specific to the chemical (e.g., air quality standards, food tolerance levels) . The ATSDR “Toxicological Profiles” (ATSDR 2015) were the primary sources for this information. EPA used t he Hazardous Substance Data Bank (HSDB) (USDHHS 2015) from the National Library of Medicine’s Toxicology

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Data Network (TOXNET) as the primary source fo r chemicals without ATSDR Toxicological Profiles. Both sources are peer

- reviewed compilations of chemical information.
EPA used a dditional references , including the following, to obtain specific types of information and to supplement the information from ATSDR and the HSDB :

• EPA ’s Six

- Year Review s (drinking water data) (USEPA 2009a; USEPA 2009b)

. • FDA Total Diet Study (USFDA 2015)

. • FDA Everything Added to Food in the United States (USFDA 2013)

. • EPA National Lake Fish Tissue Study (USEPA 2009c)

. • EPA Toxic Release Inventory (USEPA 2015g)

. • International Bottled Water Association Standards of Quality (IBWA 2012)

. • NOAA Mussel Watch (NOAA 2014)

. • A dditional sources as needed.

T o determine the RSC to be used in the AWQC calculation , EPA then used the information compiled for each chemical to address the questions posed in the Exposure Decision Tree

. Some of the important items evaluated in the Exposure Decision Tree follow:

• T he adequacy of the data available for each relevant exposure source and pathway

. • T he availability of sufficient information to characterize the likelihood of exposure to relevant sources

. • W hether there are significant known or potential uses/sources other than the source of concern (i.e., ambient water and fish/seafood from those waters)

. • W hether information o n each source is available to make a characterization of exposure
. In cases where there is a lack of environmental or exposure data, or both, the Exposure Decision Tree approach results in a r ecommended RSC of 20 percent. This 20 percent value for the RSC may be replaced where sufficient data are available to develop a scientifically defensible alternative value. When appropriate, if scientific data demonstrating that sources and routes of expo sure other than water and fish from inland and nearshore waters are not anticipated for the pollutant in question, the RSC may be raised to 80 percent based on the available data (USEPA 2000a)

. 6.2 Chemical

- specific RSC
Gamma

- HCH is an insecticide that , until 2006 , when its registration was voluntarily cancelled,
wa s used on fruits, vegetables, grains, forest crops, and animals and in animal premises. Gamma

- HCH is presently used as prescription medication for scabies and head lice (ATSDR 2005; USEPA 2006)

. Due to its pharmaceutical uses, dermal exposure could also be a source of gamma - HCH to those using it for a prescribed treatment. Because of its prior use on various crops and persistence, ingestion from f ood is a potential source of gamma - HCH exposure to the

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general populati on. In addition, surface water, finished drinking water, and ingestion of fish and shellfish are possible sources of gamma

- HCH exposure.
Gamma

- HCH is regulated under the Safe Drinking Water Act (SDWA) , and EPA’s drinking water standard (maximum contaminant level) is 0.2 μg/L (USEPA 2014c )

. Gamma

- HCH has previously been detected in treated drinking water : with mean concentrations of 23 ng/L and 147 ng/L in 1978; a mean concentration of 0.01 ng/L in 1976; and a mean concentration of 0.2 ng/L in 1972 (ATSDR 2005)

. Prior to its cancellation as a pesticide, gamma

- HCH was a target analyte in EPA’ s Six

- Year Reviews (1996

– 2002) and was detected in some drinking water , groundwater , and surface water samples (USEPA 2009a; USEPA 2009b) . There is a Standard of Quality for bottled water for gamma - HCH of 0.2 μg/L (IBWA 2012) . Therefore, based on available information regarding detection, ingestion of surface water and finished drinking water is a potentially significant source of exposure to gamma

- HCH

. The log K ow for gamma - HCH is 3.72 (ATSDR 2005) . The national

- level BAF estimates for gamma HCH range from 1,200 L/kg (TL2) to 2,500 L/kg (TL4), which indicates gamma

- HCH has a high potential for bioaccumulation (USEPA 2011b) . Gamma

- HCH was not a target analyte in NOAA’s Mussel Watch Survey (NOAA 2014) . EPA's National Lake Fish Tissue Study included gamma

- HCH as a target analyte , and it was detected in predator and bottom

- dwelling fish (USEPA 2009c)

. More recent exposure information regarding concentrations of gamma

- HCH in fish and shellfish from inland and nearshore waters and ocean fish and shellfish could not be identified. Thus, based on its potential to bioaccumulate, ingestion of fish and shellfish is a potentially significant source of exposure to gamma - HCH

. The 2004

– 2005 FDA Total Diet Study detected trace amounts of gamma

- HCH in food samples including chocolate chip cookies, brownies, and candy bars (USFDA 2005) . According to ATSDR (2005) , gamma - HCH was also previously detected in ready

- to

- eat foods in an FDA study conducted between 1982 and 1991 ( an average concentration of 0.0012 μg/L). Recent monitoring data indicate that food may no longer be a significant exposure source for the chemical . In 2013, gamma

- HCH was not detected in fruit and vegetables, drinking water , or groundwater , as reported by the Pesticide Data Program (USDA 2 014) . EPA no longer sets a 40 CFR p art 180 pesticide tolerance for this chemical in food and feed commodities (USGPO 2015) . Based on its more recent information regarding detection of gamma

- HCH , exposure to gamma - HCH from ingestion of food is not considered likely. Air is a potential source of gamma

- HCH exposure (ATSDR 2005) . Recent data from EPA’s Toxic Release Inventory indicate that 9,079 pounds of gamma

- HCH were released in 2013; however, all sources appear to have been at disposal facilities (USEPA 2015g) . Gamma

- HCH has been previously detected in the air near highways, in cities, and adjacent to industrial manufacturing plants (ATSDR 2005)

. It also was detected in samples collected between 1979 and 1980 , with a mean concentration of 0.23 ng/m 3 ; in troposphere air samples in 1985 with a mean concentration of 0.509 ng/m 3 ; and in 1988 , with a mean concentration of 0.021 ng/m 3 . S amples collected in 1996 and 1997 had a mean concentration of 0.050 ng/m 3 , and samples collected between 1990 and 1997 had average concentrations of 0.024

– 0.062 ng/m 3 (ATSDR 2005)

.

14Gamma

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Inhalation exposure is also possible via showering, bathing, or cooking with contaminated water (ATSDR 2005) . The vapor pressure of gamma

- HCH (4.2 × 10 -5 mm Hg at 20 °C) indicates that volatilization is not an important fate pr ocess for this chemical (ATSDR 2005)

. EPA lists g amma - HCH as a hazardous air pollutant (USEPA 2013) . Based on its limited use , exposure to gamma - HCH from air is possible but not considered likely. In summary, based on the physical properties and available exposure information for gamma

HCH , drinking water and fish and shellfish from inland and nearshore waters are likely to be the most significant sources of gamma

- HCH exposure. Although gamma

- HCH has been detected in food and air in the past, its limited use as a pharmaceutical product is expected to limit exposure from these sources to the general population. Therefore, the most significant routes of exposure to the general population are expected to be from ingestion of fish and shellfish from inland and nearshore waters and drinking water . L imited exposure is also pos sible from inhalation and dermal contact. Following the Exposure Decision Tree in EPA’s 2000 Methodology (USEPA 2000a) , there is not likely to be significant potential sources other than fish and shellfish from inland and nearshore waters and water ingestion (Box 7 in the Decision Tree). Th erefore, EPA recommends an RSC of 50 percent (0. 5 0) for gamma - HCH

. 7 Criteria Derivation : Analysis Table 1 summarizes the model inputs used to derive the 2015 updated human health AWQC that are protective of exposure to gamma - HCH from consuming drinking wate r and eating fish and shellfish (organisms) from inland and nearshore waters

. The c riteria calculations are presented below. These updated criteria recommendations are based on the 2000 Methodology (USEPA 2000a) and the updated exposure assumptions described above . ( See section 4, Exposure Factors ; section 5, Hazard Identification and Dose Response; and section 6, Relative Source Contribution.)

Table 1. Summary of I nput P arameters for 2015 H uman H ealth AWQC for gamma - HCH Input Parameter Value RfD 0.0047 mg/kg

- d CSF No data RSC 0.5 0 BW 80 .0 kg DI 2.4 L/d

FCR TL2 0.0076 kg/d TL3 0.0086 kg/d TL4 0.0051 kg/d

BAF TL2 1,200 L/kg TL3 2,400 L/kg TL4 2,500 L/kg

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7.1 AWQC for N oncarcinogenic Toxicological E ffects For consumption of water and organisms: AWQC (μg/L) = toxicity value (RfD [mg/kg

- d] × RSC) × B W (kg) × 1 , 000 ( μg/mg) DI (L/d) + ∑ (FCR i (kg/d) × BAF i (L/kg)) 4 i=2

= 0.0047 mg/kg

- d × 0.5 0 × 80.0 kg × 1 , 000 μg/mg

2.4 L/d + (( 0.0076 kg/d × 1,200 L/kg) + ( 0.00 86 kg/d × 2,400 L/kg) + ( 0.00 51 kg/d × 2,500 L/kg))

= 4.186 μg/L

= 4.2 μg/L (rounded) For consumption of organisms only: AWQC (μg/L) = toxicity value (RfD [mg/kg

- d] × RSC) × B W (kg) × 1 , 000 ( μg/ m g) ∑ (FCR i (kg/d) × BAF i (L/kg))

4 i=2

= 0.0047 mg/kg

- d × 0.5 0 × 80.0 kg × 1 , 000 μg/mg

( 0.0076 kg/d × 1,200 L/kg) + ( 0.0086 kg/d × 2,400 L/kg) + ( 0.00 51 kg/d × 2,500 L/kg)

= 4.422 μg/L

= 4.4 μg/L (rounded)
7.2 AWQC for C arcinogenic Toxicological E ffects
EPA identif ied no CSF sources through the systematic search described above. (See section 5, Hazard Identification and Dose Response .) Therefore, EPA was unable to derive AWQC for carcinogenic toxicological effects.
7.3 AWQC Summary EPA derive d the AWQC for gamma - HCH using a noncarcinogenic toxicity endpoint. The updated human health AWQC for gamm a- HCH are 4.2 μg/L for consumption of water and organisms and 4.4 μg/L for consumption of organisms only (Table 2). These updated criteria replace EPA’s previously published values (USEPA 2003b)

.
Table 2. Summary of EPA’s Previously Recommended (2003 ) and U pdated ( 2015 ) Human H ealth AWQC for gamma

- HCH 2003 Human Health AWQC 201 5 Human Health AWQC

Water and Organism 0.98 μg/L 4.2 μg/L Organism Only 1.8 μg/L 4.4 μg/L

The se AWQC are intended to be protective of the general adult population from noncarcinogenic effects due to chronic (up to a lifetime) e xposure to gamma - HCH from ingesting water and/or consuming fish and shellfish from inland and nearshore waters.

16Gamma

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8 Criteria Characterization The updated 2015 human health AWQC for gamma - HCH take into account current data on health effects and exposure input parameters, consistent with the 2000 Methodology (USEPA 2000a)

. The following paragraphs describe the individual influence of each of the revised inputs and exposure assumptions on the overall change in value. Body Weight EPA’s updated AWQC assume a higher BW compared to the previously recommended 2002 criteria, reflecting a recent rise in average adult BW among the U.S. population. The updated BW assumption of 80.0 kg, based on recent survey data from the 1999−2006 NHANES data, is 10 kg greater than the previous assumption of 70 kg. Assuming all other input parameters remain constant, a higher average BW in the AWQC calculations (Eq s . 1 and 2 above) results in higher AWQC. That is, as BW increases, the level of a contaminan t in water at or below which negative health effects are not anticipated from a lifetime of exposure also increases. Drinking Water Intake The updated DI assumption is 2.4 L/d , which is higher than the previo usly recommended rate of 2 L/d . Assuming all other input parameters remain constant, a higher DI assumption in the AWQC calculations (Eq s . 1 and 2 above) results in lower AWQC. That is, as DI increases, and thus overall exposure increases, the level of a contaminant in water at or below which negative h ealth effects are not anticipated from a lifetime of exposure decreases. Fish Consumption Rate The updated FCR for fish and shellfish from inland and nearshore waters is 22.0 g/d; the TL

- specific FCRs are 7.6 g/d, 8.6 g/d, and 5.1 g/d for TLs 2, 3, and 4, respectively. The previously recommended FCR was 17.5 g/d. Assuming all other input parameters remain constant, a higher FCR assumption in the AWQC calculations (Eqs. 1 and 2 above) results in lower AWQC. That is, as fish consumption increases, and thus overall exposure increases, the level of a contaminant in water at or below which negative health effects are not anticipated from a lifetime of exposure decreases.
Bioaccumulation Factor The national lower (TL2), mid (TL3), and upper (TL4) trophic level BAF s used in the updated AWQC (Eqs. 1 and 2 above) are 1,200, 2,400, and 2,500 L/kg wet

- weight, respectively. These BAFs were derived using EPA’s 2000 Methodology (USEPA 2000a) and its Technical Support Document, Volume 2 (USEPA 2003a) . These national trophic level BAFs replace EPA’s previously recommended BCF of 130 L/kg. As an additional line of evidence, EPA used model

- estimated BAFs from the Estimation Progr am Interface (EPI) Suite (USEPA 2012) to support field

- measured or predicted BAFs developed using the four methods described above. The BCFBAF p rogram within EPI Suite estimates fish BAF s by using K ow and biotransformation data from a model designed by Arnot and Gobas ( 2003) . The

17Gamma

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model includes mechanistic processes for bioaccumulation , such as chemical uptake from the water at the gill surface and from the diet, chemical elimination at the gill surface, fecal egestion, growth dilution , and metabolic biotransformation. Other processes included in the calcula tions are bioavailability in the water column (only the freely dissolved fraction can bioconcentrate) and absorption efficiencies at the gill and in the gastrointestinal tract. The model requires the K ow of the chemical and the normalized whole

- body metabo lic biotransformation rate constant as input parameters to predict BAF values. The EPI Suite model estimates are as follows :
TL 2 = 934.9 L/kg TL 3 = 1,118 L/kg TL 4 = 1,935 L/kg
Assuming all other input parameters remain constant, higher BAFs or BCF s result in lower AWQC. That is, as bioaccumulation or bioconcentration of a contaminant in fish and shellfish increases, the level of a contaminant in water at or below which negative health effects are not anticipated from a lifetime of exposure decreases.
The ut ilization of a national

- level BAF rather than a BCF better represents the amount of a contaminant accumulating in an organism because it accounts not only for the organism’s exposure to the pollutant in the water column, but also from the food chain and su rrounding environment as well as biotransformation of the pollutant in the organism due to metabolic processes. The utilization of the three trophic levels of fish and shellfish consumed, as opposed to representing all trophic levels of fish and shellfish consumed by a single value, allows for better exposure representation.
Reference Dose EPA selected an RfD of 0.0047 mg/kg

- d for gamma

- HCH based on a 2002 EP A O PP RED (USEPA 2002c) . This RfD replaces the previous value of 0.0003 mg/kg

- d (USEPA 2003b)

. E PA used th e RfD of 0.0047 mg/kg

- d to derive AWQC for non carcinogenic effects. Assuming all other input parameters remain constant, an increase in the RfD in the AWQC calculations (Eqs. 1 and 2) results in higher AWQC. Cancer Slope Factor EPA did not select a CSF for gamma - HCH and t herefore did not derive AWQC for carcinogenic effects. E PA did not derive AWQC for carcinogenic effects of gamma - HCH in its previous criteria update (USEPA 2003b)

. Relative Source Contribution An RSC of 50 percent is included in the AWQC calculation. This is higher than the 20 percent RSC used in the previous AWQC (USEPA 2003b)

. Assuming all other input parameters remain constant, a higher RSC in the AWQC calculations (Eqs. 1 and 2) results in higher AWQC.

18Gamma

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9 Chemical Name and Synonyms

• Gamma

- HCH (Lindane) (CAS Number 58

- 89

- 9)

• Aalindan

• Aficide

• Agris ol G

- 20

• Agronexit

• Ameisenatod

• Ameisenmittel Merck

• Aparasin

• Aphtiria

• Aplidal

• Arbitex

• BBH

• Ben

- Hex

• Bentox 10

• Gamma

- Benzene Hexachloride

• Benzene Hexachloride

- Gamma

- Isomer

• Bexol

• BHC

• Gamma

- BHC

• Celanex

• Chloresene

• Codechine

• Cyclohexane, 1,2,3,4,5,6

- Hexachloro

- , Gamma

- Isomer

• DBH

• Detmol

- Extrakt

• Detox 25

• Devoran

• Dol Granule

• Drill Tox

- Spezial Aglukon

• ENT 7,796

• Entomoxan

• Exagama

• Forlin

• Gallogama

• Gamacarbatox

• Gamacid

• Gamaphex

• Gamene

• Gamiso

• Gamma

- Col

19Gamma

- HCH 58

- 89 -9

• Gammahexa

• Gammahexane

• Gammalin

• Gammalin 20

• Gammaterr

• Gammex

• Gammexane

• Gammopaz

• Gexane

• HCCH

• HCH

• Gamma

- HCH

• Heclotox

• Hexa

• Hexachloran

• Hexachlorane

• Gamma

- Hexachlorane

• Gamma

- Hexachloran

• Gamma

- Hexachlor

• Gamma

- Hexachlorobenzene

• 1,2,3,4,5,6

- Hexachlorocyclohexane

• 1- Alpha,2

- Alpha,3

- Beta,4

- Alpha,5

- Alpha,6

- Beta

- Hexachlorocyclohexane

• Hexachlorocyclohexane, Gamma -
• Gamma

- 1,2,3,4,5,6

- Hexachlorocyclohexane

• 1,2,3,4,5,6

- Hexachlorocyclohexane, Gamma

- Isomer

• Hexachlorocyclohexane, Gamma

- Isomer

• Hexatox

• Hexaverm

• Hexicide

• Hexyclan

• HGI

• Hortex

• Inexit

• Isotox

• Jacutin

• Kokotine

• Kwell

• Lendine

• Lentox

• Lidenal

• Lindafor

• Lindagam

20Gamma

- HCH 58

- 89 -9

• Lindagrain

• Lindagranox

• Lindane

• Gamma

- Lindane

• Lindapoudre

• Lindatox

• Lindosep

• Lintox

• Lorexane

• Milbol 49

• Mszycol

• NA 2761

• NCI

- C00204

• Neo

- Scabicidol

• Nexen Fb

• Nexit

• Nexit

- Stark

• Nexol -E

• Nicochloran

• Novigam

• Omnitox

• Owadziak

• Pedraczak

• Pflanzol

• Quellada

• RCRA Waste Number U129

• Sang Gamma

• Silvanol

• Spritz

- Rapidin

• Spruehpflanzol

• Streunex

• TAP 85

• Tri -6

• Viton

21Gamma

- HCH 58

- 89 -9

10 References Amyes, S. 1989. Lindane: Combined Oncogenicity and Toxicity Study by Dietary Administration to Wistar Rats for 104 Weeks: Final Report . Laboratory project no. 90/CIL002/0839: CIL/002/LIN. Unpublished study prepared by Life Science Research Ltd. 3451 pp. Arnot, J.A., and A.P.C. Gobas. 2003. A Generic QSAR for Assessing the Bioaccumulation Potential of Orga nic Chemicals in Aquatic Food Webs. QSAR & Combinatorial Science 22:337

– 345. Arnot, J.A., and A.P.C. Gobas. 2006. A review of bioconcentration factor (BCF) and bioaccumulation factor (BAF) assessments for organic chemicals in aquatic organisms. Environment al Reviews 14(4):257

– 297.
ATSDR. 2005. Toxicological Profile for Alpha

- , Beta

- , Gamma

- , and Delta

- Hexachlorocyclohexane

. U.S. Department of Health and Human Services, U.S. Public Health Service, Agency for Toxic Substances and Disease Registry, Atlanta, GA. Accessed February 2015. http://www.atsdr.cdc.gov/toxprofiles/tp43.pdf

. ATSDR. 2015. Toxic Substances Portal . U.S. Department of Health and Human Services, U.S. Public Health Service, Agency for Toxic Substances and Disease Registry, Atlanta, GA. Accessed February 2015. http://www.atsdr.cdc.gov/toxprofiles/index.asp

. CalEPA. 1999. Public Health Goal for Lindane in Drinking Water . Cal ifornia Environmental Protection Agency, Office of Environmental Health Hazard Assessment. Accessed March 2015. http://oehha.ca.gov/water/phg/pdf/lindan_f.pdf

. CalEPA. 2014. All Public Health G oals . California Environmental Protection Agency, Office of Environmental Health Hazard Assessment. Accessed February 2015. http://www.oehha.ca.gov/water/phg/allphgs.html

. Environment Canada. 2006. Bioaccumulation Canada. In The OECD QSAR Toolbox, Version 3.3.2. An online database. Retrieved January 5, 2015. Organisation for Economic Co

- operation and Development, Paris, France. http://www.oecd.org/chemicalsafety/risk

- assessment/theoecdqsartoolbox.htm

. HC. 2015. Health Canada . Home page. Health Canada, Ottawa, Ontario. Accessed February 2015. http://www.hc

- sc.gc.ca/index

- eng.php

. IBWA. 2012. International Bottled Water Association Bottled Water Code of Practice (Revised December, 2012) . International Bottled Water Association, Alexandria, VA. Accessed January 2015.

22Gamma

- HCH 58

- 89 -9

NOAA. 2014. National Centers for Ocean and Coastal Science, National Status and Trends Data Portal (NCCOS NS&T Data Portal) . U.S. Department of Commerce, National Oceanic and Atmospheric Administration, National Centers for Ocean and Coastal Science, Silver Spring, MD. Accesse d March 2015. http://egisws02.nos.noaa.gov/nsandt/index.html#

. USACE. 2015. Biota

- Sediment Accumulation Factor Database . U.S. Army Corps of Engineers, Engineer Research and Development Center, Vicksburg, MS. Accessed March 2015. http://el.erdc.usace.army.mil/bsafnew/BSAF.html

. USDA. 2014. Pesticide Data Program

— Annual Summary, Calendar Year 2013 . U.S. Department of Agriculture, Wash ington, DC. Accessed March 2015. http://www.ams.usda.gov/AMSv1.0/getfile?dDocName=STELPRDC5110007

. USDHHS. 2010. Hexachlorocyclohexanes (CASRN: 608

- 73

- 1) . Hazardous Substances Data Bank, a TOXNET database. Retrieved February 18, 2015. U.S. Department of Health and Human Services, National Institutes of Health, U.S. National Library of Medicine, Bethesda, MD. http://toxnet.nlm.nih.gov/cgi

- bin/sis/search2/r?dbs+hsdb:@term+@DOCNO+1606

. USDHHS. 2015. TOXNET . Home page. Hazardous Substances Data Bank, a TOXNET database. U.S. Department of Health and Human Services, National Institutes of Health , U.S. National Library of Medicine, Bethesda, MD. Accessed January 2015. http://toxnet.nlm.nih.gov/newtoxnet/hsdb.htm

. USEPA. 1986. Gamma

- Hexachlorocyclohexane (gamma

- HCH) (CASRN 58

- 89

- 9) . Integ rated Risk Information System. Oral RfD assessment verificat ion date January 22, 1986. U.S. Environmental Protection Agency, Office of Research and Development, Washington, DC. Accessed March 2015. http://www.epa.gov/iris/subst/0065.htm

. USEPA. 1999. Guidelines for Carcinogen Risk Assessment

. Review Draft. NCEA -F- 0644. U.S. Environmental Protection Agency, Washington, DC. Accessed February 2015. http://ofmpub.epa.gov/eims/eimscomm.getfile?p_download_id=437005

. USEPA. 2000a. Methodology for Deriving Ambient Water Quality Criteria for the Protection of Human Health (2000) . EPA

- 822 -B- 00

- 004. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, DC. Accessed February 2015. http://water.epa.gov/scitech/swguidance/standards/upload/2005_05_06_criteria_hum anhealth_method_complete.pdf

. USEPA. 2000b. Methodology for Deriving Ambient Water Quality Criteria for the Protection of Human Health (2000), Technical Suppo rt Document. Vol. 1, Risk Assessment . EPA

- 822 -B00

- 005. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, DC. Accessed February 2015. http://water.epa.gov/scitech/swguidance/standards/criteria/health/methodology/uplo ad/2005_05_06_criteria_humanhealth_method_supportdoc.pdf

.

23Gamma

- HCH 58

- 89 -9

USEPA. 2001. Cancer Assessment D ocument: Evaluation of the Carcinogenic Potential of Lindane . PC. Code: 009001. Final Report. U.S. Environmental Protection Agency, Office of Pesticide Program.
USEPA. 2002a. Estimated Per Capita Fish Consumption in the United States . EPA

- 821 -C- 02

- 003. U.S . Environmental Protection Agency, Office of Water, Washington, DC. Accessed February 2015. http://water.epa.gov/scitech/swguidance/standards/criteria/health/upload/consumpti on_report.pdf

. USEPA. 2002b. National Recommended Water Quality Criteria: 2002 . EPA

- 822 -R- 02

- 047. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, DC. Accessed February 2015. http://water.epa.gov/scitech/swguidance/standards/upload/2008_04_29_criteria_wqct able_nrwqc

- 2002.pdf

. USEPA. 2002c. Reregistration Eligibility Decision for Lindane . U.S. Environmental Protection Agency, Office of Pesticide Programs, Washington, DC. Accessed May 2015. http://www.regulations.gov/#!documentDetail;D=EPA

- HQ

- OPP

- 2002

- 0202

- 0027

. USEPA. 2003a. Methodology for Deriving Ambient Water Quality Criteria for the Protection of H uman Health (2000), Technical Support Document. Vol. 2, Development of National Bioaccumulation Factors . EPA

- 822 -R- 03

- 030. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, DC. Accessed March 2015. http://www.epa.gov/scipoly/sap/meetings/2008/october/methodology.pdf

. USEPA. 2003b. National Recommended Water Quality Criteria for the Protection of Human Health. U.S. Environme ntal Protection Agency. Federal Register, December 31, 2003, 68:75507

– 75515. Accessed February 2015. http://www.gpo.gov/fdsys/pkg/FR

- 2003

- 12

- 31/html/03

- 32211.htm

. USEPA. 2006. Lindane Voluntary Cancellation and RED Addendum Fact Sheet

. U.S. Environmental Protection Agency, Washington, DC. Accessed May 2015. http://www.epa.gov/pesticides/reregistration/REDs/factsheets/lindane_fs_addendum. htm

. USEPA. 2009a. Contaminant Occurrence Support Document for Category 1 Contaminants for the Second Six

- Year Review of National Primary Drinking Water Regulations . EPA 815 -B- 09

- 010. U.S. Environm ental Protection Agency, Office of Water, Office of Ground Water and Drinking Water, Washington, DC. Accessed March 2015. http://water.epa.gov/lawsregs/rulesregs/regulatingcontaminants/sixyearreview/second _review/upload/6YearCategory1Report.pdf

.

24Gamma

- HCH 58

- 89 -9

USEPA. 2009b. Contaminant Occurrence Support Document for Category 2 Contaminants for the Second Six

- Year Review of National Primary Drinking Water Regulations . EPA

- 815 -B- 09

- 011. U.S. Environmental Protection Agency, Office of Water, Office of Ground Water and Drinking Water, Washington, DC. Accessed March 2015. http://water.epa.gov/lawsregs/rulesregs/regulatingcontaminants/sixyearreview/second _review/upload/6YearCategory2Report_final.pdf

. USEPA. 2009c. The National Study of Chemical Resid ues in Lake Fish Tissue . EPA

- 823 -R- 09

- 006. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, DC. Accessed March 2015. http://water.epa.gov/scitech/swguidance/fishstudies/upload/2009_9_28_fish_study_ data_finalreport.pdf

. USEPA. 2011a. Exposure Factors Handbook: 2011 Edition . EPA

- 600 -R- 09

- 052F. U.S. Environmental Protection Agency, Office of Re search and Development, Washington, DC. Accessed February 2015. http://www.epa.gov/ncea/efh/pdfs/efh

- complete.pdf

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