STUDY NO. 00-4206

SPONSOR STUDY NO. 211-TBA-1G

GASOLINE TBA VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION

TOXICITY STUDY IN RATS

Final Report

Submitted to: American Petroleum Institute

1220 L Street, Northwest

Washington, D.C. 20005-4070

Attn: Thomas M. Gray, M.S., D.A.B.T.

Date: 1 September 2011

Page 1 of 652



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Gasoline TBA Vapor Condensate: A One-Generation

Whole-Body Inhalation Reproduction Toxicity Study in Rats

SUMMARY

This study was designed to assess the potential reproductive toxicity of Gasoline TBA 

Vapor Condensate when administered via whole-body inhalation exposures to Sprague 

Dawley rats (26/sex/group). The test substance was administered (using a nitrogen 

enriched air mixture) at target concentrations of 2000, 10000 and 20000 mg/m3 for 

6 hours/day, 7 days/week for 10 weeks before mating, for up to 2 weeks during mating, 

for 3 weeks during gestation and for 4 weeks during lactation prior to weaning. In 

addition, a control group (26/sex) received nitrogen enriched air only while in chamber. 

Exposure levels were determined using an infra-red spectrophotometer 4 times per 

chamber per day. Additionally, the test substance’s major components were assayed once 

per chamber per week. Particle size distribution measurements were also made once per 

chamber per week using a TSI Aerodynamic Particle Sizer. 

Viability checks were performed twice daily to check for mortality and signs of severe 

toxic or pharmacologic effects. Physical observations and body weight measurements 

were made at least once pretest and at least weekly during the study. Feed consumption 

measurements were obtained beginning the week prior to treatment initiation and at least 

weekly during the study. After completion of ~ 16 weeks of exposures, all parental male 

animals (P0 generation) were sacrificed. All parental female animals (P0 generation) 

were sacrificed on Lactation Day 28. Selected organs were weighed and organ/body 

weight and organ/brain weight ratios calculated. Complete macroscopic postmortem 

examinations were performed on all parental animals. Histopathological evaluations of 

selected tissues were conducted on randomly selected parental animals.

Pups (F1 generation) were observed as soon as possible after delivery for their sex, the 

number of live and dead pups and pup abnormalities. Thereafter, litters were observed 

twice daily. On the 4th day after parturition, litters with more than ten pups were 

randomly culled to ten pups with sex distribution equalized when possible. Pups were 

given a gross physical examination, were weighed and were sexed frequently until 

weaning on the 28th day after parturition at which time they were sacrificed. A 

macroscopic postmortem evaluation and the weighing of selected organs were performed 

on selected pups. 

The mean (± standard deviation) analytical exposure concentrations of Gasoline TBA 

Vapor Condensate were determined to be 0 ± 0, 2015 ± 176, 10254 ± 355 and 

20382 ± 928 mg/m3 for the Air Control and the exposure groups, respectively. The 
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SUMMARY

analytically measured exposure levels of the airborne test substance were acceptably 

close, in the opinion of the study director, to the targeted exposure levels. The measured 

and nominal concentrations varied somewhat (less than 11%), but reasonably, from the 

expected 1:1 ratio for this type of vapor exposure. Chamber environmental conditions 

averaged 24oC temperature and 45% relative humidity. Particle sizing results indicated 

that the atmospheres were essentially vapor only, as expected, since there was no 

substantial difference between the particulate levels in the test substance chambers and 

the Air Control chamber. Analysis of the major components in the neat test substance 

and the test atmospheres showed an acceptably close, in the opinion of the study director,

comparison between the neat test substance and the vaporized test substance. This data 

demonstrated that the test animals were exposed, as expected, to all of the major 

components of the test substance in their reasonably proper proportion. The data was 

consistent from week-to-week during the study indicating stability of the test substance 

and the atmosphere generation techniques. 

There was no effect of treatment on survival, clinical observations, body weights and feed 

consumption except an exposure-related decrease in body weight gain was seen in the 

20000 mg/m3 animals during the premating period. There was no effect of treatment on 

estrous cycle (as measured by cycle length and number of estrous cycles), mating indices 

for the male rats and mating or fertility or gestation indices for the female rats except the 

animals in the 20000 mg/m3 group took slightly longer to mate than those in the other 

groups. There were no treatment-related differences in the other reproductive parameters 

up to the time of parturition including the percent of females completing delivery and the 

duration of gestation, when compared to the Air Control group. There were also no 

treatment-related differences in all parturition parameters including the total number of 

pups delivered, the number of pups dying, the viability (4 day survival) and lactation 

(21 day survival) indices, the number of implantation sites per litter, the pup sex ratio and 

the number of live pups/litter, when compared to the Air Control group. Mean percent 

motility, epididymal sperm count, homogenization-resistant testicular sperm count, sperm 

morphology and caudal epididymis weight were not affected by treatment with the test 

substance at an exposure level of 20000 mg/m3. 

There were no exposure-related differences in terminal estrous and macroscopic 

postmortem evaluations in the test substance exposed animals compared to the Air 

Control animals. Exposure-related effects on organ weights included statistically 

significant increases in kidney weights (absolute and/or relative to body and brain weight) 

at the 2 higher exposure levels in the males. These differences for the males were 

consistent with the hydrocarbon nephropathy findings at these exposure levels in the 
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SUMMARY

13-week inhalation study with this same test substance. This species- and gender-specific 

change has been well documented in male rats exposed to a variety of hydrocarbon 

compounds and is not considered relevant to humans. Statistically significant decreases 

were seen in spleen weights (absolute and/or relative to body and brain weight) at all 

exposure levels in the males and females. No other test substance related microscopic 

changes were noted in male and female reproductive organs or other protocol-specified 

tissues in this study.

The pups were generally unremarkable during the lactation period. There were no 

exposure-related differences in body weights or weight gains in the pups feeding from 

test substance exposed animals in the 2000 or 10000 mg/m3 groups, compared to the pups 

feeding from Air Control animals. Early in lactation, up to about post-natal day 14, a 

slight but statistically significant deficit in pup weight gain occurred at 20000 mg/m3. 

There were no exposure-related differences in macroscopic postmortem evaluations in the 

pups feeding from test substance exposed animals compared to the pups feeding from Air 

Control animals. However, there were exposure-related decreases in spleen weights in 

the pups feeding from the 20000 mg/m3 exposed animals compared to the pups feeding 

from Air Control animals.

In conclusion, exposure of rats to 2000, 10000 and 20000 mg/m3 of vapor of Gasoline 

TBA Vapor Condensate resulted in decreased body weight gains in the 20000 mg/m3

exposed parental animals during the premating interval and decreased spleen weights in 

the 2000, 10000 and 20000 mg/m3 exposed parental animals at their termination. 

Increases in kidney weights in parental male animals exposed to the 2 higher exposure 

levels of vapor were consistent with hydrocarbon nephropathy seen in similar animals 

exposed for 13 weeks to this test substance. However, this finding has been generally 

accepted (US EPA 1991. Alpha 2 microglobulin: association with chemically induced 

renal toxicity and neoplasia in male rat. In Risk Assessment Forum, p.85. US Govt. 

Printing Office, Washington, DC.) not to be relevant to human risk assessment. 

Therefore, a no observable adverse effect level (NOAEL) was not determined. There was 

no effect at any of the exposure levels on reproductive performance in the study, 

including mating, fertility, parturition, lactation and offspring survival except a slightly 

extended mating period for the 20000 mg/m3 test substance exposed animals and

decreases in spleen weights in pups feeding from 20000 mg/m3 test substance exposed 

animals were seen. Therefore, with respect to reproductive performance, a no observable 

effect level (NOEL) of 10000 mg/m3 of Gasoline TBA Vapor Condensate was also 

concluded with an NOAEL of 20000 mg/m3. 
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TABLE OF CONTENTS 

COVER PAGE .....................................................................................................................1 

STATEMENT OF COMPLIANCE .....................................................................................2 

SIGNATURE PAGE ...........................................................................................................3 

QUALITY ASSURANCE STATEMENT ..........................................................................4 

SUMMARY .........................................................................................................................5 

TABLE OF CONTENTS .....................................................................................................8 

1. INTRODUCTION ................................................................................................11 

2. MATERIALS AND METHODS

2.1. Study Management ....................................................................................11 

2.2. Study Dates ................................................................................................11 

2.3. Experimental Outline .................................................................................13 

2.4. Test Substance ...........................................................................................14 

2.5. Test Animals ..............................................................................................15 

2.6. Animal Assignment ...................................................................................16 

2.7. Animal Identification .................................................................................17 

2.8. Veterinary Care ..........................................................................................17 

2.9. Animal Husbandry During Non-Exposure Periods ...................................17 

2.10. Test Substance Administration and Chamber Operation ...........................20 

2.11. Experimental Evaluations ..........................................................................20 

2.12. Mating, Gestation and Lactation Procedures .............................................21 

2.13. Postmortem ................................................................................................23 

2.14. Sperm Count, Motility and Morphology Assessments ..............................27 

2.15. Statistical Analysis .....................................................................................28 

2.16. Data Storage ...............................................................................................29 

2.17. Regulatory References ...............................................................................30 

2.18. Protocol Deviations ....................................................................................30 

3. RESULTS AND DISCUSSION

3.1. Chamber Monitoring ..................................................................................32 

3.2. Parental Data ..............................................................................................33 

3.3. Pup Data .....................................................................................................38 

3.4. Postmortem Data ........................................................................................39 

4. CONCLUSION ....................................................................................................42 

REFERENCES ..................................................................................................................43
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TABLE OF CONTENTS 

CALCULATIONS .............................................................................................................44 

GENERAL PREFACE ......................................................................................................46 

TABLES

1. Summary of Survival and Pregnancy ............................................................................... 47 

2. Summary of Weekly Clinical Observations - (Premating/Mating/Postmating)) ............ 48 

3. Summary of Clinical Observations During Gestation ..................................................... 54 

4. Summary of Maternal Clinical Observations During Lactation ..................................... 55 

5. Mean Body Weight Values – (Premating/Mating/Postmating) ...................................... 56 

6. Mean Body Weight Gain - (Premating /Mating/Postmating) ......................................... 61 

7. Summary of Gestation Body Weights ............................................................................. 67 

8. Summary of Gestation Body Weight Gain ...................................................................... 68 

9. Summary of Maternal Lactation Body Weights .............................................................. 69 

10. Summary of Maternal Lactation Body Weight Gain ....................................................... 70 

11. Mean Feed Consumption (grams/kg/day) - (Premating/Postmating) ............................ 71 

12. Summary of Gestation Feed Consumption (grams/kg/day) ........................................... 75 

13. Summary of Lactation Feed Consumption (grams/kg/day) ........................................... 76 

14. Summary of Estrous Stages .............................................................................................. 77 

15. Summary of Cohabitation Data ........................................................................................ 78 

16. Summary of Delivery and Litter Data .............................................................................. 79 

17. Summary of Pup Clinical Observations During Lactation .............................................. 83 

18. Summary of Mean Pup Body Weights ............................................................................ 84 

19. Summary of Parental Necropsy Observations ................................................................. 90 

20. Summary of Pup Necropsy Observations 

Summary of Necropsy Observations for Dead Pups ....................................................... 95 

Summary of Necropsy Observations for Stillborn Pups ................................................. 97 

Summary of Necropsy Observations for Culled Pups ..................................................... 99 

Summary of Necropsy Observations for Cannibalized Pups ........................................ 100 

Summary of Necropsy Observations for Weanling Pups .............................................. 101 

21. Summary of Parental Organ Weights ............................................................................ 102 

22. Summary of Pup Organ Weight Data ............................................................................ 115 

23. Lesion Incidence Summary with Expanded Severity Levels ........................................ 119 
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TABLE OF CONTENTS 

APPENDICES 

A. Inhalation Report ......................................................................................................... 125

B. Analytical Report ......................................................................................................... 175

C. Individual Animal Termination History ..................................................................... 206 

D. Individual Weekly Clinical Observations - (Premating/Mating/Postmating) ............ 214 

E. Individual Clinical Observations During Gestation ................................................... 239 

F. Individual Maternal Clinical Observations During Lactation .................................... 247 

G. Individual Body Weights - (Premating/Mating/Postmating) ..................................... 259 

H. Individual Body Weight Gain - (Premating/Mating/Postmating) .............................. 275 

I. Individual Gestation Body Weights ............................................................................ 291 

J. Individual Gestation Body Weight Gain .................................................................... 295 

K. Individual Maternal Lactation Body Weights ............................................................ 299 

L. Individual Maternal Lactation Body Weight Gain ..................................................... 303 

M. Individual Feed Consumption (grams/kg/day) - (Premating/Postmating) ................. 307 

N. Individual Gestation Feed Consumption (grams/kg/day) .......................................... 315 

O. Individual Maternal Lactation Feed Consumption (grams/kg/day) ........................... 319 

P. Individual Estrous Cycle and Mating Data ................................................................. 323 

Q. Individual Mating Assignments .................................................................................. 331 

R. Individual Delivery and Litter Data ............................................................................ 335 

S. Individual Pup Sex and Status During Lactation ........................................................ 339 

T. Individual Pup Clinical Observations During Lactation ............................................ 343

U. Individual Pup Body Weights ..................................................................................... 383 

V. Individual Terminal Estrous Determinations .............................................................. 407

W. Individual Parental Necropsy Observations ............................................................... 411

X. Individual Pup Necropsy Observations ...................................................................... 434

Y. Individual Parental Organ and Final Body Weights and Ratios ................................ 466

Z. Individual Pup Organ Weights .................................................................................... 495

AA. Individual Animal Gross and Microscopic Observations .......................................... 528 

BB. Sperm Analysis Report ...........................................................................................571 

CC. Certificates of Analysis – (Feed, Water and Bedding) ...........................................585 

DD. Protocol and Protocol Amendments ........................................................................... 616 

EE. Historical Control Data ............................................................................................... 648 

FF. Testing Facility Personnel ........................................................................................... 651 

GG. Report Amendments .................................................................................................... 652 
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1. INTRODUCTION 

This reproductive study was designed to provide general information concerning 

the effects of Gasoline TBA Vapor Condensate on the integrity and performance 

of the male and female reproductive systems, including gonadal function, the 

estrous cycle, mating behavior, conception, gestation, parturition and lactation, 

and to provide information about the effects of the test substance on neonatal 

morbidity and mortality, and data on prenatal and postnatal developmental 

toxicity. 

2. MATERIALS AND METHODS 

2.1. STUDY MANAGEMENT 

2.1.1. SPONSOR 

American Petroleum Institute (API) 

1220 L Street, Northwest 

Washington, DC 20005-4070 

2.1.2. SPONSOR REPRESENTATIVE 

Thomas M. Gray, M.S., D.A.B.T. 

2.1.3. TESTING FACILITY 

Huntingdon Life Sciences (HLS) 

P.O. Box 2360 

Mettlers Road 

East Millstone, New Jersey 08875-2360 

2.1.4. STUDY DIRECTOR 

Gary M. Hoffman, B.A., D.A.B.T. 

2.2. STUDY DATES 

2.2.1. STUDY INITIATION 

02 August 2002 (Date Study Director signed the Protocol) 
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2.2.2. DATE OF ANIMAL RECEIPT 

06 August 2002 

2.2.3. EXPOSURE INITIATION 

20 August 2002 (Experimental Start Date) 

2.2.4. MATING INITIATION 

28 October 2002 

2.2.5. EXPOSURE TERMINATION 

28 December 2002 

2.2.6. TERMINAL SACRIFICE 

P0 Males: 11 – 12 December 2002 

P0 Females and F1 Pups: 17 – 29 December 2002 

2.2.7. EXPERIMENTAL TERMINATION DATE 

31 August 2011 (Date of last data collection = Date final report is 

signed by the Pathologist) 

2.2.8. STUDY COMPLETION 

1 September 2011 (Date Study Director signed the Final Report) 
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2.3. EXPERIMENTAL OUTLINE 

Group 

Group 

Designation 

Exposure 

Levels 

(mg/m3) a

Number of Animals 

Mated Adults 

Microscopic 

Pathology Adult 

Generationsb

Macroscopic 

Examinat

P0 P0

M F M F F

I Control 0 (air only) 26 26 10 10 3/sex

II Low 2,000 26 26 0 0 3/sex

III Middle 10,000 26 26 0 0 3/sex

IV High 20,000 26 26 10 10 3/sex

aExposures daily (7 days/week) for 6 hours per day. bHistologic examinations were performed on reproductive tissues for the control and high-exposu

M = Male; F = Female; The first day of exposure was Day 0. 
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2.4. TEST SUBSTANCE 

Gasoline TBA Vapor Condensate 

2.4.1. SUPPLIER 

Chevron Global Technology Services Company 

100 Chevron Way 

Richmond, CA 94802 

2.4.2. LOT NUMBER 

API 01-07 

2.4.3. PURITY/ANALYTICAL CONCENTRATION 

100% Gasoline TBA Vapor Condensate 

2.4.4. DESCRIPTION 

Colorless Liquid 

2.4.5. DATES RECEIVED 

18 March 2002 and 05 August 2002 

2.4.6. EXPIRATION DATE 

Not available, stable per MSDS. The test substance stability was 

tested concurrently with the study and the results (see Appendix B) 

showed it to be stable for the duration of the study. 

2.4.7. ANALYSIS 

Documentation of the identity, strength, composition, and method 

of synthesis, fabrication and/or derivation of the batch of the test 

substance and the maintenance of these records was the 

responsibility of the Sponsor. The Sponsor conducted a purity 

analysis of the test substance by GC prior to the start of this study. 

2.4.8. STORAGE 

Ambient conditions in an outdoor solvent shed or in an indoor 

laboratory. 
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2.4.9. DISPENSING 

The test substance was received from Chevron Global Technology 

Services Company in 6 of the 100 gallon cylinders. Since only 5 

gallon cylinders were practical to be used for exposure operations, 

the test substance was dispensed, as needed, at the Testing Facility 

from the 100 gallon cylinders into 5 gallon cylinders using 

nitrogen pressurization. 

2.4.10. ARCHIVAL SAMPLE 

A 50 mL sample from the lot of test substance is stored in the 

Archives of the Testing Facility under ambient conditions in an 

indoor laboratory. 

2.4.11. DISPOSITION 

The unused portion of the test substance, as well as any empty test 

substance containers, were returned to the Sponsor. Empty test 

substance containers also were returned to the Sponsor on an as 

needed basis. The Sponsor was responsible for tracking their 

disposition. 

2.5. TEST ANIMALS 

Albino Rats (Outbred) VAF/Plus

2.5.1. SPECIES 

Sprague-Dawley derived CD®

[Crl: CD® IGS BR] 

2.5.2. SUPPLIER 

Charles River Laboratories 

Kingston, New York 12484 

2.5.3. JUSTIFICATION FOR TEST ANIMAL SELECTION 

The rat was used as a surrogate for humans in the detection of 

reproductive effects and is a species in which known reproductive 

toxicants have been detected. The rat is a rodent animal model 

commonly utilized in reproduction studies and is recommended in 
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the referenced regulatory guidelines. In addition, a historical 

control database with this strain of animal and supplier facility is 

available for comparative evaluation. 

2.5.4. NUMBER OF ANIMALS 

Received: 

220 total (110 males, 110 females) 

Placed on test: 

208 total (104 males, 104 females) 

Females were nulliparous and non-pregnant. 

2.5.5. AGE AT RECEIPT 

Approximately 28 days. 

2.5.6. AGE AT INITIATION OF EXPOSURES 

Approximately 42 days. 

2.5.7. WEIGHT AT INITIATION OF EXPOSURES (GRAMS) 

Mean Range

Male: 183 160-209 

Female: 167 146-191 

2.5.8. ACCLIMATION PERIOD 

Animals were acclimated for 14 days. All animals were checked 

for viability twice daily. All animals were examined during the 

acclimation period to confirm suitability for study. 

2.6. ANIMAL ASSIGNMENT 

More animals than required for the study were purchased and acclimated. 

Animals considered suitable for the study on the basis of pretest physical 

examinations and body weight data were randomly assigned, by sex, to 

control or treated group, in an attempt to equalize mean group body 

weights. Individual weights of animals placed on test did not exceed 20% 

of the mean weight for each sex. Disposition of all animals not utilized in 

the study is maintained in the study file. 
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2.7. ANIMAL IDENTIFICATION 

Each rat was assigned a temporary identification number upon receipt. 

After selection for study (P0 generation), each rat was ear-tagged with a 

number assigned by the Testing Facility. This assigned animal number 

plus the study number comprised the unique animal number for each 

animal. If the tag was lost, it was replaced. In addition, each cage was 

provided with a cage card that was color-coded for exposure level 

identification and contained the study number and animal number 

information. 

2.8. VETERINARY CARE 

Animals were monitored by the technical staff for any conditions 

requiring possible veterinary care. 

2.9. ANIMAL HUSBANDRY DURING NON-EXPOSURE PERIODS 

2.9.1. FACILITIES MANAGEMENT/ANIMAL HUSBANDRY 

Currently acceptable practices of good animal husbandry were 

followed e.g., Guide for the Care and Use of Laboratory Animals; 

National Academy Press, 1996. Huntingdon Life Sciences, East 

Millstone, New Jersey is fully accredited by the Association for 

Assessment and Accreditation of Laboratory Animal Care 

International (AAALAC). 

2.9.2. HOUSING 

Animals were housed in suspended, stainless steel cages with wire 

mesh fronts and floors. Animals were doubly housed during the 

initial week of acclimation and individually housed thereafter, 

except as follows. 

Mating: One male and one female were co-housed continuously 

(except during treatment) until mating occurred or for a maximum 

of 14 days. Mating pairs were assigned randomly. 

Lactation: Dam and litter were housed together in a solid plastic 

“shoebox” cage until weaning. 
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2.9.3. FEED 

Certified Rodent Diet, No. 5002; (Meal) (PMI Nutrition 

International, St. Louis, Missouri) was available without restriction 

except during exposures. 

2.9.4. FEED ANALYSIS 

Analysis of each feed lot used during this study was performed by 

the manufacturer. Results can be found in Appendix CC. There 

were no known contaminants in the feed which were expected to 

interfere with the results of this study. 

2.9.5. WATER 

Water (Elizabethtown Water Company, Westfield, New Jersey) 

was available without restriction, except during exposures, via an 

automated watering system. 

2.9.6. WATER ANALYSIS 

Water analyses are conducted by Elizabethtown Water Company, 

Westfield, New Jersey (Raritan-Millstone Plant) to ensure that 

water meets standards specified under the EPA Federal Safe 

Drinking Water Act Regulations (40 CFR Part 141). In addition, 

water samples are collected biannually from representative rooms 

in the Testing Facility; chemical and microbiological water 

analyses are conducted on these samples by a subcontract 

laboratory (Lancaster Laboratories, Lancaster, PA). Results of all 

water analyses can be found in Appendix CC. There were no 

known contaminants in the water that were expected to interfere 

with the results of this study. 

2.9.7. BEDDING SUBSTANCE 

Ground corncob bedding (Bed-O’-Cobs® 1⁄4 inch irradiated, The 

Anderson’s Inc., Maunee, OH), was provided for each mated 

female on Day 18 of gestation. Fresh bedding was provided at 

least weekly and as needed until Lactation Day 28. 
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2.9.8. BEDDING ANALYSIS 

Analyses for a representative batch of bedding as used on study 

can be found in Appendix CC. There were no known 

contaminants in the bedding that were expected to interfere with 

the results of this study. 

2.9.9. ENVIRONMENTAL CONDITIONS 

Light/Dark Cycle 

A twelve-hour light/dark cycle controlled by an automatic timer 

was provided. 

Temperature 

Temperature was monitored in accordance with Testing Facility 

SOPs and maintained within the specified range to the maximum 

extent possible. Excursions outside the specified range were 

generally brief in duration and considered not to have affected the 

integrity of the study. 

Desired: 19 to 25°C 

Actual: 14.3 to 26.0°C 

Average: 17.7 to 24.1°C 

Relative Humidity 

Relative humidity was monitored in accordance with Testing 

Facility SOPs and maintained within the specified range to the 

maximum extent possible. Excursions outside the specified range 

were generally brief in duration and considered not to have 

affected the integrity of the study. 

Desired: 30 to 70% 

Actual: 14.26 to 99.81% 

Average: 23.46 to 75.42% 
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Air Changes 

Air changes were maintained at a rate of 10-15 per hour range to 

the maximum extent possible and were recorded once prior to 

exposures. 

Desired: 10 to 15 per hour 

Actual: 11.5 to 14.6 per hour 

2.10. TEST SUBSTANCE ADMINISTRATION AND CHAMBER 

OPERATION 

Inhalation (whole-body exposures). 

Details of test article administration and chamber operation information 

are presented in the Inhalation Report (Appendix A). 

2.11. EXPERIMENTAL EVALUATIONS 

2.11.1. VIABILITY EXAMINATION (CAGESIDE) 

Observations for mortality and signs of severe toxic or 

pharmacological effects were made at least twice daily (morning 

and afternoon). 

2.11.2. DETAILED PHYSICAL EXAMINATIONS 

The animals were observed as a group at least once during each 

exposure. 

In addition, each parental animal was removed from its cage and 

examined at least once pretest, at the study start (first day of 

treatment), and weekly thereafter during the study period. Females 

were then observed weekly until there was evidence of mating. 

Once mated, females were observed on Gestation Days 0, 7, 14, 

and 20 and on Lactation Days 1, 4, 7, 14, 21, and 28. Females that 

showed evidence of mating but did not deliver were observed on 

Gestation Days 0, 4, 7, 14, and 20 and were euthanized on 

Gestation Day 25. Females without evidence of mating were 

observed weekly until euthanized on the last possible Gestation 

Day 25. Examinations included, but were not limited to, 

observations of general condition, skin and fur, eyes, nose, oral 

cavity, abdomen and external genitalia, as well as evaluations of 
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respiration and palpation for tissue masses. Physical examinations 

were performed post-exposure except during the gestation and 

lactation periods when they were performed pre-exposure. 

2.11.3. BODY WEIGHT 

Each parental animal was removed from its cage and weighed at 

least once pretest, at the time of randomizing into study groups, on 

the day treatment initiated, and weekly thereafter during the study 

period (including the mating period) and prior to termination. 

Once mated, females were weighed on Gestation Days 0, 4, 7, 14, 

and 20 and on Lactation Days 1, 4, 7, 14, 21, and 28. Females that 

did not show evidence of mating and/or did not deliver pups were 

weighed weekly until euthanasia. 

2.11.4. FEED CONSUMPTION 

Feed consumption was measured once pretest and weekly 

thereafter during the study period. Once mated, female feed 

consumption was measured on Gestation Days 0, 4, 7, 14, and 20 

and on Lactation Days 1, 4, 7, 14, 21, and 28 until euthanasia. 

Females without evidence of mating and/or that did not deliver 

pups had food consumption measured weekly until euthanasia. 

Feed consumption was not measured for any animal during the 

mating period when males and females were co-housed. 

2.12. MATING, GESTATION AND LACTATION PROCEDURES 

2.12.1. ESTROUS CYCLING 

Daily vaginal smears were taken at approximately the same time 

each day, and the stage of estrus was determined for each P0

female beginning three weeks prior to cohabitation and continuing 

until there was evidence of mating or until the 14-day mating 

period ended. Care was taken to reduce the induction of pseudopregnancy. 

2.12.2. MATING PROCEDURE 

Following a 10-week pre-mating treatment period, one male and 

one female (non-siblings) from the same exposure group were 

caged together until evidence of mating was observed (a 
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copulation plug in the vagina and/or microscopic observation of 

sperm in the vaginal smear) or 14 consecutive days had elapsed. 

Males and females were not caged together during the mating 

period while being exposed. The day evidence of mating was 

observed was defined as Day 0 of gestation. If mating did not 

occur after 14 days, the animals were separated without further 

opportunity for mating. 

2.12.3. PARTURITION AND LACTATION 

On Day 18 of gestation, several days prior to expected parturition, 

each female was transferred to a solid plastic “shoe-box” cage. 

Bedding substance was provided and changed at least weekly and 

as needed. Examinations for signs of parturition were made twice 

daily (morning and afternoon). The day on which parturition was 

first observed was defined as Day 0 of lactation. Females that 

exhibited no evidence of mating were transferred to plastic “shoebox” cages when the first animals mated reached their Day 18 of 

gestation. 

2.12.4. LITTER EVALUATIONS (F1) 

Observations 

F1 litters were observed as soon as possible after delivery for the 

number of live and dead pups, sex of the pups and pup 

abnormalities. The sex was determined on the day of delivery 

completion for all pups by inspection of the ano-genital distance. 

All pups were uniquely identified within the litter by toe tattoo on 

the day of delivery completion. Thereafter, litters were observed 

twice daily (morning and afternoon) for the presence of dead pups 

which were removed for examination. Litter size was recorded 

daily from Lactation Day 1 to 28. 

Culling of Pups 

On Day 4 of lactation, each litter with more that 10 pups was 

culled to that number, with sex distribution equalized (five/sex) 

when possible. The pups were culled randomly. Preferential 

culling of runts was not performed. 
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Physical Examinations 

Each F1 pup was given a physical examination on the day of 

delivery completion and on Day 4, 7, 14, 21 and 28 of lactation. 

Body Weight 

Individual F1 pup weights were recorded on Day 1, 4 (precull 

intervals), 7, 14, 21 and 28 of lactation (postcull intervals). 

Sexing 

The sex of each pup was recorded on the day of delivery 

completion, then reconfirmed on Lactation Day 4, 7, 14, 21, and 

28. 

2.13. POSTMORTEM 

2.13.1. PARENTAL ANIMALS (P0) 

Necropsy was performed on all 26 animals/sex/group. All P0

males were euthanized after the last F1 litters were delivered. All 

P0 females with litters were euthanized on Day 28 of lactation. P0

females with no confirmed day of mating were euthanized on the 

last possible Gestation Day 25. P0 females with confirmed days of 

mating that did not deliver were euthanized on Gestation Day 25. 

Method of Euthanasia 

Exsanguination following anesthesia with inhaled carbon dioxide. 

Macroscopic Examination 

Complete macroscopic postmortem examinations were performed 

on all adult animals, including animals found dead and dams with 

total litter loss. All abnormal observations were recorded. The 

necropsy of the parental animals included examination of the 

external surface and all orifices; the external surfaces of the brain 

and spinal cord; the organs and tissues of the cranial, thoracic, 

abdominal and pelvic cavities and neck; and the remainder of the 

carcass. Examination of all P0 females included a vaginal smear to 

determine the stage of the estrous cycle and a count of uterine 

implantation scars, if present. 
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Organ Weights 

Organs indicated in Table I (page 25) were weighed for all adult 

animals at the scheduled sacrifice intervals, except for dams which 

did not produce a litter or had total litter loss. Prior to weighing, 

the organs were carefully dissected and properly trimmed to 

remove adipose and other contiguous tissues in a uniform manner. 

Organs were weighed as soon as possible after dissection in order 

to avoid drying. Paired organs were weighed together (gonads 

were also weighed separately). Organ weight data are presented as 

absolute values and relative to terminal body weight and brain 

weight. 

Tissues Preserved and Examined Histopathologically 

The tissues listed in Table I (page 25) were obtained at the 

scheduled sacrifice intervals and preserved for all animals, except 

for dams which did not produce a litter or had total litter loss. 

Slides of the indicated tissues were prepared and examined 

microscopically for 10 randomly selected animals in the control 

and high exposure groups. 

Histopathological examinations of the testes were conducted to 

identify potential treatment-related effects such as retained 

spermatids, missing germ cell layers or types, multinucleated giant 

cells, or sloughing of spermatogenic cells into the lumen. The 

examination of the intact epididymis was of a longitudinal section 

that permitted examination of the caput, corpus and cauda regions, 

to identify such lesions as sperm granulomas, leukocytic 

infiltration (inflammation), aberrant cell types within the lumen, or 

the absence of clear cells in the cauda epididymal epithelium. 

Histopathological examination of the ovary included evaluation of 

five sections taken at least 100 μm apart from the inner third of 

each ovary. These examinations were to detect depletion of the 

primordial follicle population and enumerated the total number of 

primordial follicles for comparison with the ovaries from control 

animals. These examinations were also to confirm the presence or 

absence of growing follicles and corpora lutea in comparison to 

control ovaries. 
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TABLE I 

ORGAN NAME WEIGHED PRESERVED 

EXAMINED 

MICROSCOPICALLY

(Groups I and IV) 

adrenal glands X X X 

brain (medulla, pons, cerebrum and 

cerebellum) X X 

epididymides (total and caudal) X Xa Xa 

kidneys X X 

liver X X 

lungs (with mainstem bronchi) X X X 

ovaries X X X 

pituitary gland X X X 

prostate gland X X X 

seminal vesicles with coagulating glands 

(and fluids) X X X 

spleen X X 

testes X Xa Xa 

uterus (body/horns) with cervix and 

oviducts X X X 

vagina X X 

tissues with macroscopic findings 

including tissue masses 

 

X X 

target organs X X 

a

Right testis and epididymis only unless a macroscopic abnormality was noted on the left testis or left 

epididymis, then the left testis and left epididymis were preserved instead for possible histopathology. 

Preservatives 

All tissues - 10% neutral buffered formalin (NBF). 

Testes and epididymides were placed in Modified Davidson’s 

solution initially and then retained in 10% formalin. Lungs were 

infused with formalin prior to their immersion into a larger volume 

of the same fixative. 

Processing 

After fixation, the tissues and organs from randomly selected 

animals (10/sex/Groups I and IV) were routinely processed, 
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embedded in paraffin, cut at a microtome setting of 4-7 microns, 

mounted on glass slides and stained with hematoxylin and eosin. 

2.13.2. F1 PUPS AND WEANLINGS 

Method of Euthanasia 

F1 pups (on Lactation Day 4) were euthanized via an 

intraperitoneal injection of sodium pentobarbital. F1 pups (after 

Lactation Day 4) and weanlings were euthanized by 

exsanguination after anesthesia with inhaled carbon dioxide. 

Dead and Culled Pups 

F1 pups found dead at birth were identified as stillborn or born 

alive but found dead (lung floatation test). F1 pups found dead 

during the lactation period were examined to the maximum extent 

possible for defects and/or the cause of death, and for the presence 

or absence of milk in the stomach. Dead pups were preserved 

intact in 10% NBF. Partially cannibalized pups were examined to 

the maximum extent possible and discarded. Culled F1 pups were 

examined for external abnormalities. If unremarkable, these pups 

were then euthanized and discarded. Culled F1 pups with external 

abnormalities were preserved intact in 10% NBF. 

Terminal Necropsy: 

Macroscopic Examination and Tissues Preserved 

Macroscopic examinations were performed on up to 3 randomly 

selected pups/sex/litter on Day 28 of lactation. Examinations 

included observations of any structural abnormalities or 

pathological changes. Special attention was given to the organs of 

the reproductive system, brain, liver, kidneys, pituitary, adrenal 

gland, spleen, heart and thymus gland. 

All remaining F1 pups were examined for external abnormalities 

and euthanized. Pups with external abnormalities were preserved 

intact in 10% NBF. Pups with no external abnormalities were 

discarded. No further macroscopic postmortem examinations were 

performed. 
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Organ Weights 

The following organs were weighed from one randomly selected 

pup/sex/litter sacrificed on Day 28 of lactation: 

brain 

spleen 

thymus gland 

Organ weight data are presented as absolute values and relative to 

terminal body weight and brain weight. 

2.14. SPERM COUNT, MOTILITY AND MORPHOLOGY 

ASSESSMENTS 

All P0 males euthanized at termination in each group had the following 

sperm evaluations available for analysis: 1) motility; 2) a count of 

homogenization-resistant testicular sperm; 3) a count of caudal epididymal 

sperm; and 4) sperm morphology (cauda epididymis). Evaluations were 

performed by Pathology Associates International, Frederick, MD (PAI) as 

follows: 

The right testis and right epididymis from each animal were removed 

intact, weighed (testes weighed together and also separately) and fixed in 

modified Davidson’s solution for at least 48 hours prior to permanent 

storage in 10% NBF. 

The left epididymis was removed intact, weighed, and frozen on dry ice 

for transport to PAI. The epididymides were stored frozen at -70°C until 

evaluation for caudal sperm count. Each epididymis was thawed and the 

caudal portion removed and weighed. A homogenized sample of the 

caudal epididymis was stained and then examined using the Hamilton 

Thorne IVOS sperm analyzer. For each stained preparation, 20 fields 

were counted. The total number of sperm in the caudal epididymis was 

calculated and adjusted for the caudal epididymal weight. Additionally, 

for each male, two sperm morphology slides were prepared, stained with 

Eosin and evaluated for morphological development. 

Note: If a macroscopic abnormality was noted on the left testis or left 

epididymis, then the right epididymis was transferred to PAI and the left 

testis and left epididymis was preserved as indicated for possible 

histopathology. 
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The left vas deferens was excised and placed in a prewarmed solution of 

phosphate buffered saline and 1% Bovine Serum Albumin. After a 

minimum three minute “swimout” period, a sample was placed in a 

Hamilton Thorne IVOS sperm analyzer. Five fields were selected and 

stored as digital images. The images were analyzed for percent motility 

and transferred to optical media for permanent storage. 

The left testis was removed and frozen on dry ice for transport to PAI. 

The testis were stored frozen at -20°C until processed for counting of 

homogenization-resistant sperm. 

(See Appendix BB for details and methodology.) 

Tissues retained from these evaluations will be discarded following 

issuance of the final report following consultation with the Sponsor. The 

Testing Facility was responsible for the GLP compliance of this 

subcontractor. 

2.15. STATISTICAL ANALYSIS 

2.15.1. CONTINUOUS DATA 

The following parameters were analyzed statistically: 

Body weights 

Body weight change 

Feed consumption values 

Organ weight data 

Estrous cycle data 

Gestation length 

Pup body weights 

Number of pups (live, dead, total) 

Method of Analysis 

Mean values of all exposure groups were compared to the mean 

value for the control group at each time interval. 

One-way analysis of variance (ANOVA) was applied (Dunlap and 

Duffy, 1975). If a significant F-ratio indicated inequality of group 

means, Dunnett’s test (Dunlap et al., 1981) was applied to 
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determine which group(s), if any, differed significantly from 

control. 

All tests were conducted at the 5% and 1% significance levels. 

2.15.2. SPERM AND MOTILITY ANALYSIS 

The following parameters were analyzed statistically: 

Mean sperm count (homogenization-resistant testicular sperm 

count and caudal epididymal sperm count) and motility data.

A Kruskal-Wallis non-parametric ANOVA test was performed to 

assess significance (Kruskal and Wallis, 1952, 1953). If a 

significant difference occured (p<0.05), the Wilcoxon (MannWhitney U) test (Kruskal, 1957) was used for pair-wise 

comparisons of each treated group to the vehicle control group. 

Statistics were performed using an IBM compatible computer with 

SAS computer programs (SAS/STAT User’s Guide, 1989). 

2.15.3. INCIDENCE DATA 

The following parameters were analyzed statistically: 

Mortality rates 

Mating indices (male and female) 

Pregnancy rates 

Male fertility indices 

Live birth indices 

Pup viability indices (Days 0-4) and lactation indices (Days 4-28) 

Incidence Data Analysis 

A Fisher Exact Test with Bonferroni correction was performed to 

identify differences between the groups (Siegel, 1956). All 

statistical tests were conducted at the 5% and 1%, two-sided risk 

levels. 

2.16. DATA STORAGE 

All raw data, preserved specimens, and retained samples, as well as the 

original study protocol and the original final report are to be maintained in 

the Archives of the Testing Facility upon completion of the study. 
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All raw data and all reports generated by Pathology Associates 

International (PAI), Frederick, MD were shipped to Huntingdon Life 

Sciences for archival storage. 

The Sponsor will determine the final disposition of these materials. 

2.17. REGULATORY REFERENCES 

2.17.1. TEST GUIDELINE 

This study was designed to meet or exceed the pertinent 

requirements of: 

US EPA Vehicle Emissions Inhalation Exposure Guideline 79.61, 

CFR Vol. 59, No. 122, 27 June 1994. 

US EPA (Environmental Protection Agency) Health Effects Test 

Guidelines, OPPTS 870.3800, Reproduction and Fertility Effects 

(August 1998) except this study will only continue through the 1st 

generation of offspring. 

2.17.2. GOOD LABORATORY PRACTICES 

This study was conducted in accordance with US EPA 79.60, CFR 

Vol. 59, No. 122, 27 June 1994 and was performed according to 

the protocol and Huntingdon Life Sciences’ and Pathology 

Associates International’s Standard Operating Procedures. 

2.17.3. ANIMAL WELFARE ACT COMPLIANCE 

This study complied with all appropriate parts of the Animal 

Welfare Act regulations: 9 CFR Parts 1 and 2 Final Rules, Federal 

Register, Volume 54, No. 168, August 31, 1989, pp. 36112-36163 

effective October 30, 1989 and 9 CFR Part 3 Animal Welfare 

Standards; Final Rule, Federal Register, Volume 56, No. 32, 

February 15, 1991, pp. 6426-6505 effective March 18, 1991. 

2.18. PROTOCOL DEVIATIONS 

The following protocol deviations occurred during the study but were not 

considered to have compromised the validity or integrity of the study: 
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1. Due to a faulty timer, the twelve-hour light/dark cycle was interrupted 

from 21-Oct-02 to 24-Oct-02 with the result that the lights did not go 

off during those three nights. Only a slight but transient effect on 

body weights was noted and no apparent effect on the estrous cycle 

was noted. 

2. Due to technician error, Animal Number 1535 was not sacrificed on 

23 November 2002 (Gestation Day 25) as per protocol. The animal 

was sacrificed on 24 November 2002 (Gestation Day 26). 

3. Due to technician error, Dam Number 3528’s Pup Number 5 was not 

preserved in 10% NBF after necropsy was performed. 

4. Due to technician error, the left epididymis for Animal Number 3029 

was lost during necropsy on Study Day 113. As a result, the weight 

for left epididymis and total organ weight for this organ was not 

recorded. 

5. Due to technician error, no vaginal smear was taken prior to necropsy 

of Animal Number 1527 which was found dead on 24 November 2002 

(Lactation Day 1). 

6. Although required in the protocol, analyses for each batch of bedding 

used on study was not provided by the Supplier and therefore not 

maintained on file at the Testing Facility. However, representative lot 

numbers of bedding which were analyzed and provided by the 

Supplier are maintained on file. 

7. Due to technician error, for Dam Number 1529, a body weight and 

physical observation were not recorded on Lactation Day 1. 

8. Due to technician error, the Week 15 (Study Day 105) observations 

were not recorded for unmated female animal numbers 1548, 1550, 

2527, 2538, 2542, 3538, 3539, 3550 and 4541). 

9. Group 1 animals were removed from the whole-body exposure 

chamber after 20 minutes post-exposure on Day 5 instead of after at 

least 30 minutes. 
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3. RESULTS AND DISCUSSION 

3.1. CHAMBER MONITORING 

(Appendices A and B) 

Chamber distribution analyses (see Table IV in Appendix A) showed that 

the test substance was evenly distributed within each chamber. Chamber 

monitoring (see Table VI in Appendix A) showed that the chamber oxygen 

levels were at least 19%. Chamber room monitoring (see Table VI in 

Appendix A) showed that no test substance was present in the room and that 

the sound and light levels were acceptable. 

The target and mean (± standard deviation) analytical (IR) and nominal 

concentrations are summarized as follows: 

Group 

Test 

Substance 

Target 

Concentration 

(mg/m3) 

Analytical 

Concentration 

(mg/m3) 

Nominal 

Concentration 

(mg/m3) 

I Air Control 0 0 ± 0 0 ± 0 

II 

Gasoline TBA 

Vapor Condensate 2,000 2015 ± 176 2220 ± 113 

III 

Gasoline TBA 

Vapor Condensate 10,000 10254 ± 355 10443 ± 294 

IV 

Gasoline TBA 

Vapor Condensate 20,000 20382 ± 928 19985 ± 864 

The analytically measured exposure levels of the airborne test substance 

were reasonably close to the targeted exposure levels. The measured and 

nominal concentrations varied somewhat (less than 11%), but acceptably, in 

the opinion of the study director, from the expected 1:1 ratio for this type of 

vapor exposure. Chamber environmental conditions averaged 24oC 

temperature and 45% relative humidity. 

Mean particle size distribution measurements for the exposures are 

summarized as follows: 
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Group 

Test 

Substance 

Mass Median 

Aerodynamic 

Diameter 

(μm) 

Geometric 

Standard 

Deviation 

Total 

Mass 

Concentration 

(mg/m3) 

I Air Control 1.713 1.797 5.20 x 10-3

II 

Gasoline TBA 

Vapor Condensate 

1.792 1.785 5.12 x 10-3

III 

Gasoline TBA 

Vapor Condensate 

1.642 1.788 5.72 x 10-3

IV 

Gasoline TBA 

Vapor Condensate 

2.466 1.881 5.44 x 10-3

These results indicated that the atmospheres were essentially vapor only, as 

expected, since there was no substantial difference between the test 

substance chambers and the Air Control chamber. 

Analysis of the major components in the neat test substance and the test 

atmospheres (see Appendix B) showed an acceptably close, in the opinion 

of the study director, comparison between the neat test substance and the 

vaporized test substance. This data demonstrated that the test animals were 

exposed, as expected, to all of the major components of the test substance in 

their proper proportion. The data were consistent from week-to-week 

during the study indicating stability of the test substance and the atmosphere 

generation techniques. 

3.2. PARENTAL DATA (P0 GENERATION) 

3.2.1. MORTALITY 

(Table 1, Appendix C) 

There was no effect of treatment on survival. All animals survived 

until their scheduled termination except: 

1. Female #4549 of the 20000 mg/m3 group that was found dead 

on study day 39. This animal had no prior clinical signs or body 

weight effects but was noted at necropsy with a broken incisor, 

discolored thymus and discolored lungs. 
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2. Female #1527 of the Air Control group that was found dead on 

lactation day 1. This animal had no prior clinical signs or body 

weight effects but was noted at necropsy with a discolored thymus, 

discolored adrenals and a fetus retained in the left uterine horn. 

3. Female #2549 of the 2000 mg/m3 group and females # 3536 and 

3548 of the 10000 mg/m3 group after total litter loss on study day 

119. 

3.2.2. PHYSICAL OBSERVATION DATA 

(Tables 2, 3 and 4; Appendices A, D, E and F) 

The test animals were unremarkable in-chamber during the 

exposure periods. 

An increased incidence of alopecia of the extremities/snout was 

seen in the males at 10000 and 20000 mg/m3. Otherwise, the test 

animals were generally unremarkable during the non-exposure 

periods during the premating period in both sexes, the 

mating/postmating period in the male rats, and the gestation and 

lactation periods in the female rats. Scattered observations such as 

chromodacryorrhea were noted but were considered not treatmentrelated. 

3.2.3. BODY WEIGHTS – PREMATING PERIOD (BOTH SEXES) 

AND PREMATING/POSTMATING PERIOD (MALES) 

(Tables 5 and 6; Appendices G and H) 

Over approximately the first 3 weeks of exposure at 20000 mg/m3, 

especially the males and to a lesser extent the females showed 

statistically significantly reduced weight gain, resulting in an 

approximate 6% deficit in body weight as compared with Control. 

This deficit subsequently remained until termination for the males 

and into late lactation for the females (see section 3.2.5 below). 

Other transient weight gain differences from Control that occurred 

sporadically in all the test substance exposed groups were 

considered not exposure-related. 
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3.2.4. MATERNAL BODY WEIGHTS - GESTATION AND 

LACTATION PERIODS 

(Tables 7, 8, 9 and 10; Appendices I, J, K and L) 

The deficit in female body weight at 20000 mg/m3 (approximately 

5% as compared with Control) that was noted during the premating period persisted through gestation and into lactation (~3-

5%), but apparently began to resolve after about post-natal day 14. 

There was no difference in body weight gains among groups 

during gestation. Body weight gains were significantly increased 

in the 20000 mg/m3 group for the entire lactation period (days 1-

28). 

3.2.5. FEED CONSUMPTION – PREMATING PERIOD 

(Table 11; Appendix M) 

There were no exposure-related differences in feed consumption in 

the test substance exposed animals compared to the Air Control 

animals. While a few statistically significant differences were 

noted at various intervals, the absolute differences were generally 

small. 

3.2.6. MATERNAL FEED CONSUMPTION - GESTATION AND 

LACTATION PERIODS 

(Tables 12 and 13; Appendices N and O) 

There were no exposure-related differences in feed consumption 

during the gestation and lactation periods in the test substance 

exposed animals compared to the Air Control animals. While 

statistically significant decreases were noted at two intervals 

during the lactation period in the 2000 mg/m3 group, these were 

not considered treatment-related. 

3.2.7. ESTROUS CYCLE DATA 

(Table 14; Appendix P) 

There was no effect of treatment on estrous cycle (as measured by 

cycle length and number of estrous cycles) for the female rats. 
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During the pre-mating period, 16 females in the study (3 in the Air 

Control group, 4 in the 2000 mg/m3 group, 8 in the 10000 mg/m3 

group and 1 in the 20000 mg/m3 group) commenced extended 

periods of diestrus (10 days or greater), indicative of incidental 

pseudopregnancy. One of these females (1529) in the Air Control 

group showed persistent diestrus for 15 days (10 days premating 

and 15 days cohabitation). One of these females (3550) in the 

10000 mg/m3 group showed persistent diestrus for 24 days (10 

days premating and 14 days cohabitation). These females showed 

no signs of mating and were not pregnant. It is possible that these 

animals were “triggered” into sequential periods of 

pseudopregnancy. 

During the mating period, one female in the Air Control group 

(1548) and one female in the 20000 mg/m3 group (2547) became 

pseudopregnant at the start and did not recommence normal 

cycling before the end of the 2-week mating period. One other 

female in each of the 2000 mg/m3 (2538) and 10000 mg/m3 (3539) 

groups showed evidence of pseudopregnancy during the latter half 

of the mating period, but these 2 females nevertheless had 2 

opportunities to mate (2 estruses with the male). One female in the 

2000 mg/m3 group (2542) went into a persistent estrus state during 

the mating period (having previously shown one normal estrus 

with the male, without mating) and did not become pregnant. One 

female in the 20000 mg/m3 group (4541) during the mating period 

showed prolonged estrus interspersed with brief diestrus states, 

and again no pregnancy resulted. 

Of the 16 females that were acyclic during the premating phase, 13 

mated with 11 becoming pregnant. The pattern of incidence of 

irregular or abnormal cycles across the study was considered not to 

indicate an effect of treatment. Adequate assessment of cyclicity 

was possible despite the incidental episodes of pseudopregnancy. 

3.2.8. MATING, FERTILITY AND GESTATION INDICES 

(Tables 15 and 16; Appendices Q and R) 

Mating indices for the male rats treated with the test substance 

were comparable to the Air Control group. Mating, fertility and 

gestation indices for the female rats treated with the test substance 
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were comparable to the Air Control group. The animals in the 

20000 mg/m3 group took slightly longer to mate than those in the 

other groups. The Mating, Fertility, and Gestation Indices in each 

group were as follows: 

Parameter 

Air 

Control 

2000 

mg/m3

10000 

mg/m3

20000 

mg/m3

Number Cohabitated 26 26 26 25 

Number Mated 24 23 23 23 

Number of Females Missing First Opportunity to Mate 2 3 3 7 

Number of Females Missing Two or More Opportunities 

to Mate 1 3 2 1 

Number Pregnant 21 22 22 22 

Number Pregnant with Total Litter Loss 0 1 2 0 

Mating Index 92.3% 88.5% 88.5% 92.0% 

Fertility Index 87.5% 95.7% 95.7% 95.7% 

Gestation Index 100% 100% 100% 100% 

Premating Treatment Period 

 Number of Irregular Cycles1 7 10 6 11 

 Number of Acyclic Intervals2 3 4 8 1 

Cohabitation Treatment Period 

 Number of Irregular Cycles 0 2 0 1 

 Number of Acyclic Intervals 1 1 3 0 

Outcome of Females with Acyclic Intervals 

Number of Females with Persistent Estrus3 0 1 0 1 

Total Number of Females with Acyclic Intervals 4 6 10 1 

Number of Previously Acyclic Females that Mated 3 5 8 1 

Number of Previously Acyclic Females Becoming 

Pregnant 3 3 5 0 

1Irregular Cycles - Cycles of 2 to 3 days or 6, 7, 8 or 9 days 

2Acyclic Intervals - Persistent diestrus of 10 or greater days 

3Persistent Estrus - At least 4 consecutive days of estrus 

The apparent similar trend in the 10000 mg/m3 group, indicated by 

the number of pairings exceeding 4 days to mate, was an artifact of 

the pattern of (incidental) pseudopregnancy in the group. There 

were no treatment-related differences in the other reproductive 

parameters up to the time of parturition, including the duration of 

gestation, when compared to the Air Control group. 
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3.2.9. PARTURITION AND LITTER SURVIVAL DATA 

(Table 16; Appendices R and S) 

Parturition data for the female rats treated with the test substance 

were comparable to the Air Control group. There were no 

exposure-related differences in the number of conceptuses (as 

indicated by implantation sites), the number of pups delivered, the 

live litter size or the pup sex ratio. The slightly reduced group pup 

viability to post-natal day (PND) 4 at 10000 and 20000 mg/m3 was 

a result of 2 total litter losses at 10000 mg/m3 and one litter at 

20000 mg/m3 that lost 7 of 17 pups, occurrences that were 

considered incidental and not related to exposure. The number of 

stillborn were significantly increased in the 10000 mg/m3 group. 

This increase in the number of stillborn, related to the two dams 

with total litter loss, was considered incidental and not related to 

exposure since there was one dam in the 2000 mg/m3 group that 

also had total litter loss. The total litter losses were considered 

incidental since they did not occur in an exposure-related pattern. 

Overall, pup survival in all groups was within normal limits for the 

strain of animals. 

3.3. PUP DATA (F1 GENERATION) 

3.3.1. OBSERVATION DATA 

(Table 17; Appendix T) 

The pups were unremarkable during the lactation period. 

3.3.2. BODY WEIGHT DATA 

(Tables 16 and 18; Appendix U) 

There were no exposure-related differences in body weights or 

weight gains in the pups feeding from test substance exposed 

animals in the 2000 or 10000 mg/m3 groups, compared to the pups 

feeding from Air Control animals. The statistically increased pup 

weights at 2000 mg/m3 were related to the statistically significant 

decrease in litter size in this group, a common finding unrelated to 

exposure. Early in lactation, up to about post-natal day 14, a slight 

but statistically significant deficit in pup weight gain occurred at 
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20000 mg/m3, such that pup weight at PND 14 was statistically 

significantly reduced and was approximately 10% less than the 

other groups. Subsequently, weight gain was more comparable 

with Control, but at weaning (post-natal day 28 in this study) there 

remained an approximate 6% deficit in absolute pup weight at 

20000 mg/m3 (difference from Control no longer statistically 

significant). Other minor inter-group differences in pup body 

weight performance were transitory and considered to be not 

exposure-related. 

3.4. POSTMORTEM DATA 

3.4.1. TERMINAL ESTROUS AND MACROSCOPIC 

POSTMORTEM EVALUATIONS - PARENTAL 

GENERATION 

(Table 19; Appendices V, W and AA) 

The incidence of estrus or pro-estrus stages among the females in 

each group at termination indicated that a normal pattern of 

cyclicity was re-established. In 3 of the 4 groups, about onequarter of the females were in one or other of these stages, which 

is what would be expected. Only the 2000 mg/m3 group departed 

from this pattern, with 10 of the 26 animals in either estrus or proestrus. This was considered to be of no concern and within this 

laboratory’s normal range of results. 

Compared with controls there was an increased incidence of 

discolored foci in the lungs of males administered 10000 and 

20000 and in the lungs of females administered 20000 mg/m3. 

Most of these foci were tan colored and less than 0.1 cm diameter. 

In the absence of any other microscopic finding that correlated 

with the tan foci, the increased incidence in this macroscopic 

finding is considered potentially related to the test article, but nonadverse. Otherwise, there were no exposure-related differences in 

macroscopic postmortem findings in the test substance-exposed 

animals compared to the Air Control animals. 
Huntingdon Life Sciences 00-4206 Page 40 

 211-TBA-1G Final Report

3.4.2. MACROSCOPIC POSTMORTEM EVALUATIONS - PUPS 

(Table 20; Appendix X) 

There were no exposure-related differences in macroscopic 

postmortem evaluations in the pups feeding from test substance 

exposed animals compared to the pups feeding from Air Control 

animals including stillborns, pups found dead and pups 

cannibalized at the end of the weaning period. Scattered 

observations such as dilated renal pelvis were noted but were not 

treatment-related. 

3.4.3. ORGAN WEIGHTS - PARENTAL GENERATION 

(Table 21; Appendix Y) 

Exposure-related effects on organ weights included statistically 

significant increases in kidney weights (absolute and/or relative to 

body and brain weight) at the 2 higher exposure levels in the 

males. These differences for the males were consistent with the 

hydrocarbon nephropathy findings at the 20000 mg/m3 exposure 

level in the 13-week inhalation study 00-6131 with this same test 

substance. However, this finding has been generally accepted (US 

EPA 1991. Alpha 2 microglobulin: association with chemically 

induced renal toxicity and neoplasia in male rat. In Risk 

Assessment Forum, p.85. US Govt. Printing Office, Washington, 

DC.) not to be relevant to human risk assessment. 

Statistically significant decreases were seen in spleen weights 

(absolute and/or relative to body and brain weight) at all exposure 

levels in the males and/or females. Although no effect on the 

spleen was noted in the 13-week inhalation study 00-6131 

including the histopathological and immunotoxicological 

assessments, the differences in this study were considered an 

adverse effect since a similar effect was seen in the pups (see 

below) and considering that this study involved more exposures 

overall (112 vs 65) and more exposures per week (7 vs 5 days) 

than the 13-week study. Otherwise, there were no other exposurerelated differences in organ weights in the test substance exposed 

animals compared to the Air Control animals. A few other 

statistically significant differences in organ weights (absolute 
Huntingdon Life Sciences 00-4206 Page 41 

 211-TBA-1G Final Report

and/or relative to body and brain weight) were noted for some 

exposed groups. However, these were not in a treatment-related 

pattern and were in some cases related to decreased body weight 

gains earlier discussed. 

3.4.4. ORGAN WEIGHTS - PUPS 

(Table 22; Appendix Z) 

Exposure-related effects on organ weights included statistically 

significant decreases in spleen weights (absolute and relative to 

brain weight) at the 20000 mg/m3 exposure level. There were no 

other exposure-related differences in organ weights in the pups 

feeding from test substance exposed animals compared to the pups 

feeding from Air Control animals. 

3.4.5. HISTOPATHOLOGICAL EVALUATIONS - PARENTAL 

GENERATION 

(Table 23; Appendix AA) 

No test substance related microscopic changes were noted in male 

and female reproductive organs or other protocol-specified tissues 

in this study. The remaining microscopic findings were similar to 

those routinely observed in animals of this age and species and 

were considered incidental. 

3.4.6. SPERM EVALUATIONS – PARENTAL GENERATION 

(Appendix BB) 

A statistically significant decrease in the caudal epididymis weight 

was observed in the high exposure group compared to the control. 

This decrease was not considered biologically meaningful because 

there was not a similar decrease in the epididymal sperm count. 

Group mean values were 0.28 grams for the control group and 

0.26 grams for the high-exposure group. Mean percent motility, 

epididymal sperm count, homogenization-resistant testicular sperm 

count, sperm morphology and caudal epididymis weight were not 

affected by treatment at an exposure level of 20000 mg/m3

. No 

biologically meaningful differences were observed between the 

study groups. 
Huntingdon Life Sciences 00-4206 Page 42 

 211-TBA-1G Final Report

4. CONCLUSION 

Exposure of rats to 2000, 10000 and 20000 mg/m3

 of vapor of Gasoline TBA 

Vapor Condensate resulted in decreased body weight gains in the 20000 mg/m3

exposed parental animals during the premating interval and decreased spleen 

weights in the 2000, 10000 and 20000 mg/m3 exposed parental animals at their 

termination. Increases in kidney weights in parental male animals exposed to the 

2 higher exposure levels of vapor were consistent with hydrocarbon nephropathy 

seen in similar animals exposed for 13 weeks to this test substance. However, 

this finding has been generally accepted (US EPA 1991. Alpha 2 microglobulin: 

association with chemically induced renal toxicity and neoplasia in male rat. In 

Risk Assessment Forum, p.85. US Govt. Printing Office, Washington, DC.) not to 

be relevant to human risk assessment. Therefore, a no observable adverse effect 

level (NOAEL) was not determined. There was no effect at any of the exposure 

levels on reproductive performance in the study, including mating, fertility, 

parturition, lactation and offspring survival except a slightly extended mating 

period for the 20000 mg/m3 test substance exposed animals and decreases in 

spleen weights in pups feeding from 20000 mg/m3 test substance exposed animals 

were seen. Therefore, with respect to reproductive performance, a no observable 

effect level (NOEL) of 10000 mg/m3 of Gasoline TBA Vapor Condensate was 

also concluded with an NOAEL of 20000 mg/m3. 
Huntingdon Life Sciences 00-4206 Page 43 

211-TBA-1G Final Report

REFERENCES

Dunlap, W.P. and Duffy, J.A. 1975. Fortran IV functions for calculating exact 

probabilities associated with z, chi-square, t and f values. Behav. Res. Methods and 

Instrumentations 7: 59-60. 

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV functions for calculating 

probabilities associated with Dunnett’s test. Behav. Res. Methods and Instrumentation

13: 363-366. 

Kruskal, W.H. 1957. Historical Notes on the Wilcoxon Unpaired Two-Sample Test. 

Journal of the American Statistical Association 52: 356-360. 

Kruskal, W.H. and Wallis, W.A. 1952. Use of Ranks in One-Criterion Variance Analysis. 

Journal of the American Statistical Association 47: 583-621. 

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis (1952). Journal of the 

American Statistical Association 48: 907-911. 

SAS Institute. 1989. SAS/STAT User’s Guide 1989a. Version 6, Fourth Edition. Cary, 

NC: SAS Institute.

Seigel, S. 1956. Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill.
Huntingdon Life Sciences 00-4206 Page 44 

211-TBA-1G Final Report 

CALCULATIONS

Feed Consumption:

grams of feed consumed /kilograms of body weight /day (g/kg/day) = 

[(total grams of feed presented - amount of feed remaining) ÷ (average 

kilogram body weight)] ÷ no. of days 

Female Mating Index:

no. of females with confirmed mating (sperm and/or vaginal plug) plus no. of pregnant 

females without evidence of mating (no sperm or vaginal plug)/no. of females placed 

with males

Female Fertility Index:

no. of females pregnant/no. of females confirmed mating or pregnancy for females 

without evidence of mating 

Pregnancy Index:

no. of females pregnant/no. of females inseminated × 100 

Male Mating Index:

no. of males with confirmed mating with a female or pregnancy for females without 

evidence of mating/no. of males placed with females

Male Fertility Index:

no. of males mating and impregnating a female plus the no. of males with a pregnant 

female without evidence of mating/no. of males with confirmed mating plus no. of males 

with a pregnant female without evidence of mating 

Gestation Index:

no. of females with liveborn/no. of females with confirmed pregnancy 

Viability Index:

no. of pups alive Day 4 precull/no. of liveborn pups 
Huntingdon Life Sciences 00-4206 Page 45 

211-TBA-1G Final Report 

CALCULATIONS

Lactation Index: 

no. of pups alive Day 21/no. of pups Day 4 postcull 

Live Birth Index:

total no. of liveborn pups/total no. of pups born 
Huntingdon Life Sciences 00-4206 Page 46 

211-TBA-1G Final Report 

 

General Preface 

General Notes

Individual animal data values presented in this report m ay be rounded. Unrounded 

individual animal data values are used to calculate the reported m ean and standard 

deviation values. Therefore, use of the reported individual values to reproduce m eans, 

standard deviations and/or to perform any subsequent calculations m ay produce m inor 

discrepancies between the calculated values and those presented in this report. 

Key to Abbreviations 

M = Male 

F = Female 

Gen. = General 

PG = Pregnant 

Group 

Group 

Designation 

Exposure 

Levels 

(mg/m3

)

I Control 0 (air only) 

II Low 2000 

III Middle 10000 

IV High 20000 

Note: 

Dam # 1527 was found dead on Lactation Day 1 and therefore her pups were 

subsequently sacrificed. Individual data for this animal and her pups is presented in the 

appropriate appendices, but as a sum mary of litter data would be of no value, this data 

has not been included in the appropriate summary tables. 
23-JUN-2009 10:39

Huntingdon Life Sciences 00-4206 PAGE 47 

 

 TABLE 1 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF SURVIVAL AND PREGNANCY 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

No. of males at start N 26 26 26 26 

Premating

 - Died/sacrificed N 0 0 0 0 

Postmating

 - Died/sacrificed N 0 0 0 0 

No. of females at start N 26 26 26 26 

 Died/sacrificed premating N 0 0 0 1 

No. of females cohabitated N 26 26 26 25 

 - Without evidence of mating N 3 3 3 2 

 Pregnant N 1 0 0 1 

 Nonpregnant N 2 3 3 1 

Pregnant N 21 22 22 22 

 - Died/sacrificed N 0 0 0 0 

 - Died delivering N 0 0 0 0 

 - Died/sacrificed post partuma N 1 1 2 0 

 - Aborted died/sacrificed N 0 0 0 0 

Nonpregnant N 5 4 4 3 

 - Died/sacrificed N 0 0 0 0 

Total females died/sacrificed N 1 1 2 1 

 % 3.8 3.8 7.7 3.8 

Dams delivering N 21 22 22 22 

 - With liveborn pups N 21 22 22 22 

 % 100.0 100.0 100.0 100.0 

 - With all pups stillborn N 0 0 0 0 

 % 0.0 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 

a

Includes animals sacrificed following total litter loss.
Huntingdon Life Sciences 00-4206 Page 48 

211-TBA-1G Final Report 

Summary of Weekly Clinical Observations Table 2 

Total represents a cum ulative total of all animals with the indicated observation one or 

more times during the study. 

Corresponding exposure levels for each group were as follows: 

Group I - 0 mg/m3 

Group II - 2000 mg/m3

Group III - 10000 mg/m3

Group IV - 20000 mg/m3

Note: 

Observations for unmated females were not performed on Study Day 105, due to 

technician error. 
23-JUN-2009 10:42 

Huntingdon Life Sciences 00-4206  TABLE 2  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS MALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS ------------------------------------------------------------------------------------------------------------------ DAY OF STUDY  GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119 TOTAL ------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  III 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  Normal WITHIN NORMAL LIMITS I 26 26 26 25 25 25 24 23 23 23 22 21 21 21 20 20 20 21 0 26  II 26 26 26 24 25 23 22 22 21 20 20 18 18 19 19 18 18 18 0 26  III 26 26 26 24 22 23 20 20 16 16 15 15 16 16 16 15 16 19 0 26  IV 26 26 26 26 26 23 23 25 22 19 19 19 19 20 20 20 19 19 0 26  Dead TERMINAL SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26  II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26  Dermal-General ALOPECIA - I 0 0 0 1 1 1 1 1 1 1 2 3 3 3 3 3 3 2 0 3 EXTREMITIES/SNOUT II 0 0 0 0 0 0 1 1 3 4 4 4 5 3 3 3 3 3 0 5  III 0 0 0 1 3 3 6 6 10 10 10 10 9 9 9 9 9 6 0 10  IV 0 0 0 0 0 3 3 1 4 6 6 6 6 5 5 5 6 5 0 9 ULCERATION - CERVICAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  II 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ALOPECIA - GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1  II 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 
23-JUN-2009 10:42 

Huntingdon Life Sciences 00-4206  TABLE 2  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS MALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS ------------------------------------------------------------------------------------------------------------------ DAY OF STUDY  GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119 TOTAL ------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  III 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 BLACK/BROWN STAINS ON I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 SNOUT II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  Ocular CHROMODACRYORRHEA - I 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 BILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CHROMODACRYORRHEA - I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 UNILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  Oral/Buccal INCISORS MALOCCLUDED I 0 0 0 0 0 0 1 2 2 2 2 2 2 2 2 2 2 2 0 3  II 0 0 0 0 0 2 2 4 3 3 3 4 4 4 4 4 4 4 0 5  III 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1  IV 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 2 1 0 2 INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 2 2 2 0 2  II 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 3 3 3 0 4  III 0 0 0 0 0 0 0 0 0 1 2 1 1 1 1 1 1 1 0 2  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 
23-JUN-2009 10:42 

Huntingdon Life Sciences 00-4206  TABLE 2  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS MALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS ------------------------------------------------------------------------------------------------------------------ DAY OF STUDY  GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119 TOTAL ------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  III 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26  IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 ORAL SORE I 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1  II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 
23-JUN-2009 10:43 

Huntingdon Life Sciences 00-4206  TABLE 2  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS FEMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS ------------------------------------------------------------------------------------------------------------------ DAY OF STUDY  GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL ------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 21 3 3 3 3 0 2  II 26 26 26 26 26 26 26 26 26 26 26 23 4 3 3 3 0 3  III 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 3 0 3  IV 26 26 26 26 26 26 26 26 25 25 25 21 3 2 2 1 0 1  Normal WITHIN NORMAL LIMITS I 26 26 26 26 26 26 26 25 25 25 26 21 3 3 3 3 0 0 26  II 26 26 26 26 26 26 25 24 24 24 24 21 4 3 3 3 0 0 26  III 26 26 26 25 25 25 23 25 25 24 23 21 3 2 2 2 0 0 26  IV 26 26 26 26 26 26 25 24 20 20 22 18 3 2 2 1 0 0 26  Dead FOUND DEAD I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  IV 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 TERMINAL SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2  II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1  Dermal-General ALOPECIA - I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 EXTREMITIES/SNOUT II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 1 1 1 2 0 0 1 1 1 0 0 0 0 0 0 2  IV 0 0 0 0 0 0 0 0 2 2 2 2 0 0 0 0 0 0 2 ALOPECIA - GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1  IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 
23-JUN-2009 10:43 

Huntingdon Life Sciences 00-4206  TABLE 2  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS FEMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS ------------------------------------------------------------------------------------------------------------------ DAY OF STUDY  GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL ------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 21 3 3 3 3 0 2  II 26 26 26 26 26 26 26 26 26 26 26 23 4 3 3 3 0 3  III 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 3 0 3  IV 26 26 26 26 26 26 26 26 25 25 25 21 3 2 2 1 0 1 YELLOW ANO-GENITAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 STAINING II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  IV 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 2  Ocular CHROMODACRYORRHEA - I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 UNILATERAL II 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1  III 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1  IV 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1  Oral/Buccal INCISORS MALOCCLUDED I 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1  II 0 0 0 0 0 0 1 2 2 2 2 2 0 0 0 0 0 0 2  III 0 0 0 0 0 0 1 1 1 1 1 1 0 0 0 0 0 0 1  IV 0 0 0 0 0 0 1 1 1 1 2 2 0 0 0 0 0 0 2 INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0  IV 0 0 0 0 0 0 1 1 1 1 2 2 0 0 0 0 0 0 2 
23-JUN-2009 10:45

Huntingdon Life Sciences 00-4206 PAGE 54 

 

 TABLE 3 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF CLINICAL OBSERVATIONS DURING GESTATION - (frequency/animals) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 to 25

 Normal 

----------

WITHIN NORMAL LIMITS 89/23 79/21 80/21 86/22 

TERMINAL SACRIFICE 2/ 2 1/ 1 1/ 1 2/ 2 

 Dermal-General 

------------------

ALOPECIA - EXTREMITIES/SNOUT 0/ 0 2/ 1 4/ 1 3/ 2 

 Ocular 

----------

CHROMODACRYORRHEA - UNILATERAL 0/ 0 5/ 2 1/ 1 0/ 0 

 Oral/Buccal 

---------------

INCISORS MALOCCLUDED 3/ 1 7/ 3 5/ 2 4/ 1 

INCISORS BROKEN/MISSING 0/ 0 4/ 1 3/ 3 4/ 1 
23-JUN-2009 10:46

Huntingdon Life Sciences 00-4206 PAGE 55 

 

 TABLE 4 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MATERNAL CLINICAL OBSERVATIONS DURING LACTATION - (frequency/animals) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 1 to 28

 Normal 

----------

WITHIN NORMAL LIMITS 106/18 107/18 107/18 122/21 

FOUND DEAD 1/ 1 0/ 0 0/ 0 0/ 0 

TERMINAL SACRIFICE 20/20 22/22 22/22 22/22 

 Gen. Appearance 

-------------------

EARS SWOLLEN/RED - BILATERAL 0/ 0 0/ 0 6/ 1 0/ 0 

 Dermal-General 

------------------

ALOPECIA - EXTREMITIES/SNOUT 0/ 0 0/ 0 4/ 1 0/ 0 

 Ocular 

----------

CHROMODACRYORRHEA - UNILATERAL 1/ 1 8/ 2 6/ 1 0/ 0 

 Oral/Buccal 

---------------

INCISORS MALOCCLUDED 13/ 3 18/ 3 12/ 2 6/ 1 

INCISORS BROKEN/MISSING 6/ 1 17/ 3 4/ 1 10/ 2 
23-JUN-2009 10:47

Huntingdon Life Sciences 00-4206 PAGE 56 

 

 TABLE 5 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 MEAN 116 116 116 116 

 S.D. 9.1 9.1 9.2 9.5 

 N 26 26 26 26 

 DAY 0 MEAN 185 183 182 181 

 S.D. 11.2 12.6 11.9 9.8 

 N 26 26 26 26 

 DAY 7 MEAN 241 240 239 232 

 S.D. 15.2 15.1 15.5 10.4 

 N 26 26 26 26 

 DAY 14 MEAN 299 298 294 284* 

 S.D. 18.8 18.8 20.5 12.6 

 N 26 26 26 26 

 DAY 21 MEAN 348 344 338 327**

 S.D. 24.4 21.8 24.8 15.8 

 N 26 26 26 26 

 DAY 28 MEAN 377 377 369 358* 

 S.D. 28.5 25.7 25.8 17.8 

 N 26 26 26 26 

 DAY 35 MEAN 409 410 398 385**

 S.D. 33.2 27.8 28.8 20.1 

 N 26 26 26 26 

 DAY 42 MEAN 432 435 419 413 

 S.D. 36.6 30.4 31.0 23.3 

 N 26 26 26 26 

 DAY 49 MEAN 455 457 441 430* 

 S.D. 39.8 31.7 34.3 25.9 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 10:47

Huntingdon Life Sciences 00-4206 PAGE 57 

 

 TABLE 5 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 56 MEAN 468 472 458 444* 

 S.D. 41.4 33.6 35.9 26.6 

 N 26 26 26 26 

 DAY 63 MEAN 484 491 475 460* 

 S.D. 43.1 34.1 39.9 29.2 

 N 26 26 26 26 

 DAY 69 MEAN 498 503 487 468* 

 S.D. 46.2 35.6 42.1 31.2 

 N 26 26 26 26 

 DAY 77 MEAN 510 514 498 479* 

 S.D. 47.1 35.4 42.2 29.5 

 N 26 26 26 26 

 DAY 84 MEAN 523 528 511 488* 

 S.D. 50.1 35.7 47.1 33.3 

 N 26 26 26 26 

 DAY 91 MEAN 534 539 521 497**

 S.D. 48.2 37.6 47.6 35.3 

 N 26 26 26 26 

 DAY 98 MEAN 546 550 528 504**

 S.D. 51.6 38.1 49.4 35.8 

 N 26 26 26 26 

 DAY 105 MEAN 555 563 540 518* 

 S.D. 52.2 39.7 49.7 35.9 

 N 26 26 26 26 

 DAY 112 MEAN 567 573 553 531* 

 S.D. 56.8 41.1 51.6 35.7 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 10:49

Huntingdon Life Sciences 00-4206 PAGE 58 

 

 TABLE 5 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 MEAN 132 132 131 131 

 S.D. 9.0 9.0 9.1 8.9 

 N 26 26 26 26 

 DAY 0 MEAN 169 167 166 165 

 S.D. 10.9 10.9 10.1 9.8 

 N 26 26 26 26 

 DAY 7 MEAN 194 193 194 185 

 S.D. 13.7 13.4 13.8 12.2 

 N 26 26 26 26 

 DAY 14 MEAN 218 215 213 206* 

 S.D. 15.1 16.8 16.3 12.6 

 N 26 26 26 26 

 DAY 21 MEAN 237 234 232 223**

 S.D. 16.5 17.7 16.9 13.2 

 N 26 26 26 26 

 DAY 28 MEAN 248 246 244 236 

 S.D. 17.7 18.8 18.1 15.5 

 N 26 26 26 26 

 DAY 35 MEAN 261 260 254 247* 

 S.D. 19.6 21.2 18.6 16.4 

 N 26 26 26 26 

 DAY 42 MEAN 268 270 263 259 

 S.D. 21.5 21.0 18.8 15.9 

 N 26 26 26 25 

 DAY 49 MEAN 280 278 270 264* 

 S.D. 22.6 23.6 17.5 15.7 

 N 26 26 26 25 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 10:49

Huntingdon Life Sciences 00-4206 PAGE 59 

 

 TABLE 5 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 56 MEAN 284 284 277 269* 

 S.D. 23.7 23.1 19.1 16.8 

 N 26 26 26 25 

 DAY 63 MEAN 290 291 286 273* 

 S.D. 25.0 24.1 19.8 16.8 

 N 26 26 26 25 

 DAY 69 MEAN 291 293 289 275* 

 S.D. 24.6 25.3 20.6 18.1 

 N 26 26 26 25 

 DAY 77 MEAN 311 298 322 304 

 S.D. 32.1 23.9 22.9 3.9 

 N 3 4 3 3 

 DAY 84 MEAN 324 300 338 318 

 S.D. 49.8 13.5 29.7 15.3 

 N 3 3 3 2 

 DAY 91 MEAN 328 297 322 343 

 S.D. 64.9 5.7 25.5 49.9 

 N 3 3 3 2 

 DAY 98 MEAN 364 318 325 311 

 S.D. 114.4 20.2 24.5 0.0 

 N 3 4 5 1 

 DAY 105 MEAN 310 324 330 325 

 S.D. 34.6 16.6 27.4 0.0 

 N 2 4 5 1 

 DAY 112 MEAN 354 329 

 S.D. 0.0 15.7 

 N 1 2 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 10:49

Huntingdon Life Sciences 00-4206 PAGE 60 

 

 TABLE 5 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 113 MEAN 351 331 

 S.D. 0.0 24.0 

 N 1 2 

 DAY 114 MEAN 342 331 

 S.D. 0.0 11.6 

 N 1 2 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 10:51

Huntingdon Life Sciences 00-4206 PAGE 61 

 

 TABLE 6 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 TO 0 MEAN 69 67 66 65* 

 S.D. 4.9 4.6 5.1 3.3 

 N 26 26 26 26 

 DAY 0 TO 7 MEAN 56 56 57 51**

 S.D. 6.3 4.5 6.2 5.4 

 N 26 26 26 26 

 DAY 7 TO 14 MEAN 58 58 55 52**

 S.D. 7.8 6.0 8.1 5.7 

 N 26 26 26 26 

 DAY 14 TO 21 MEAN 49 46 45 43**

 S.D. 7.9 6.5 7.3 6.5 

 N 26 26 26 26 

 DAY 21 TO 28 MEAN 29 33 30 31 

 S.D. 6.8 6.9 7.2 4.7 

 N 26 26 26 26 

 DAY 28 TO 35 MEAN 32 33 30 27* 

 S.D. 7.4 6.0 6.3 6.4 

 N 26 26 26 26 

 DAY 35 TO 42 MEAN 23 24 21 28* 

 S.D. 5.3 6.9 6.0 9.9 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 10:51

Huntingdon Life Sciences 00-4206 PAGE 62 

 

 TABLE 6 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 42 TO 49 MEAN 23 22 22 17**

 S.D. 5.9 5.1 6.2 9.4 

 N 26 26 26 26 

 DAY 49 TO 56 MEAN 13 16 17 14 

 S.D. 6.4 5.0 5.7 6.3 

 N 26 26 26 26 

 DAY 56 TO 63 MEAN 16 18 17 16 

 S.D. 5.6 4.8 6.9 5.3 

 N 26 26 26 26 

 DAY 63 TO 69 MEAN 13 12 12 9* 

 S.D. 7.2 6.3 5.6 6.1 

 N 26 26 26 26 

 DAY 69 TO 77 MEAN 12 11 10 11 

 S.D. 7.2 7.3 11.8 8.2 

 N 26 26 26 26 

 DAY 77 TO 84 MEAN 13 14 13 9 

 S.D. 5.3 7.4 13.2 7.0 

 N 26 26 26 26 

 DAY 84 TO 91 MEAN 11 11 10 9 

 S.D. 6.2 4.5 5.3 7.1 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 10:51

Huntingdon Life Sciences 00-4206 PAGE 63 

 

 TABLE 6 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 91 TO 98 MEAN 13 11 7* 7* 

 S.D. 8.3 5.0 5.9 9.2 

 N 26 26 26 26 

 DAY 98 TO 105 MEAN 9 13* 12 14* 

 S.D. 5.9 7.8 4.9 7.2 

 N 26 26 26 26 

 DAY 105 TO 112 MEAN 12 9 13 13 

 S.D. 6.6 6.3 6.2 4.6 

 N 26 26 26 26 

 DAY 0 TO 69 MEAN 313 319 306 287* 

 S.D. 41.1 29.9 36.9 30.7 

 N 26 26 26 26 

 DAY 0 TO 112 MEAN 382 389 371 350* 

 S.D. 51.3 36.5 47.9 35.5 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 10:53

Huntingdon Life Sciences 00-4206 PAGE 64 

 

 TABLE 6 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 TO 0 MEAN 37 35 35 34 

 S.D. 5.3 5.6 5.8 5.7 

 N 26 26 26 26 

 DAY 0 TO 7 MEAN 25 26 28 20* 

 S.D. 5.8 6.6 6.5 7.4 

 N 26 26 26 26 

 DAY 7 TO 14 MEAN 24 22 19 21 

 S.D. 6.1 6.9 6.3 7.2 

 N 26 26 26 26 

 DAY 14 TO 21 MEAN 19 19 19 17 

 S.D. 5.8 5.6 6.4 7.1 

 N 26 26 26 26 

 DAY 21 TO 28 MEAN 11 12 12 14 

 S.D. 6.2 5.9 6.9 7.7 

 N 26 26 26 26 

 DAY 28 TO 35 MEAN 13 14 10 11 

 S.D. 5.3 6.5 6.3 8.3 

 N 26 26 26 26 

 DAY 35 TO 42 MEAN 7 10 9 11 

 S.D. 6.8 4.8 6.2 8.5 

 N 26 26 26 25 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 10:53

Huntingdon Life Sciences 00-4206 PAGE 65 

 

 TABLE 6 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 42 TO 49 MEAN 11 9 8 5**

 S.D. 6.4 6.3 7.4 7.4 

 N 26 26 26 25 

 DAY 49 TO 56 MEAN 5 6 7 5 

 S.D. 5.4 7.2 7.2 6.2 

 N 26 26 26 25 

 DAY 56 TO 63 MEAN 5 6 9 4 

 S.D. 8.1 10.2 8.1 7.4 

 N 26 26 26 25 

 DAY 63 TO 69 MEAN 2 2 3 3 

 S.D. 8.5 8.5 11.1 8.5 

 N 26 26 26 25 

 DAY 69 TO 77 MEAN 18 6 9 16 

 S.D. 13.0 8.3 19.5 12.1 

 N 3 4 3 3 

 DAY 77 TO 84 MEAN 13 14 16 13 

 S.D. 23.3 13.4 10.9 12.4 

 N 3 3 3 2 

 DAY 84 TO 91 MEAN 4 -3 -16 25 

 S.D. 23.9 8.4 7.1 34.6 

 N 3 3 3 2 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
23-JUN-2009 10:53

Huntingdon Life Sciences 00-4206 PAGE 66 

 

 TABLE 6 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 91 TO 98 MEAN 36 10 4 3 

 S.D. 53.5 4.1 11.4 0.0 

 N 3 3 3 1 

 DAY 98 TO 105 MEAN 11 6 5 14 

 S.D. 8.1 3.7 5.1 0.0 

 N 2 4 5 1 

 DAY 0 TO 69 MEAN 122 126 123 110* 

 S.D. 17.2 20.1 15.6 13.6 

 N 26 26 26 25 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 10:57

Huntingdon Life Sciences 00-4206 PAGE 67 

 

 TABLE 7 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF GESTATION BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 MEAN 294 291 283 275* 

 S.D. 26.4 26.5 19.5 15.8 

 N 20 22 22 21 

 DAY 4 MEAN 317 317 309 301 

 S.D. 27.9 29.2 19.7 19.5 

 N 20 22 22 21 

 DAY 7 MEAN 327 328 319 310 

 S.D. 30.6 29.2 20.4 19.1 

 N 20 22 22 21 

 DAY 14 MEAN 357 355 347 341 

 S.D. 30.8 30.9 21.3 21.2 

 N 20 22 22 21 

 DAY 20 MEAN 418 410 407 403 

 S.D. 33.0 33.6 31.5 28.0 

 N 20 22 22 21 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 10:57

Huntingdon Life Sciences 00-4206 PAGE 68 

 

 TABLE 8 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF GESTATION BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 0 TO 4 MEAN 23 26 27 25 

 S.D. 5.9 6.6 6.5 7.4 

 N 20 22 22 21 

 DAYS 4 TO 7 MEAN 10 11 10 10 

 S.D. 5.0 3.1 3.6 3.8 

 N 20 22 22 21 

 DAYS 7 TO 14 MEAN 30 27 28 31 

 S.D. 4.6 5.9 8.1 5.5 

 N 20 22 22 21 

 DAYS 14 TO 20 MEAN 61 55 60 62 

 S.D. 12.9 16.6 24.1 13.2 

 N 20 22 22 21 

 DAYS 0 TO 20 MEAN 124 119 125 128 

 S.D. 11.5 19.7 28.4 16.9 

 N 20 22 22 21 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 10:59

Huntingdon Life Sciences 00-4206 PAGE 69 

 

 TABLE 9 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 1 MEAN 324 319 318 307 

 S.D. 26.3 29.0 21.7 18.2 

 N 19 22 21 22 

 DAY 4 MEAN 332 327 323 321 

 S.D. 29.1 28.3 21.6 23.4 

 N 20 21 20 22 

 DAY 7 MEAN 350 334 341 333 

 S.D. 31.1 30.3 21.2 21.7 

 N 20 21 20 22 

 DAY 14 MEAN 370 355 365 350* 

 S.D. 23.9 26.0 21.3 24.3 

 N 20 21 20 22 

 DAY 21 MEAN 355 351 350 342 

 S.D. 26.2 23.7 16.1 23.2 

 N 20 21 20 22 

 DAY 28 MEAN 330 329 329 325 

 S.D. 23.4 25.6 18.6 23.2 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 11:00

Huntingdon Life Sciences 00-4206 PAGE 70 

 

 TABLE 10 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 1 TO 4 MEAN 7 9 7 14 

 S.D. 11.9 10.4 14.1 12.4 

 N 19 21 20 22 

 DAYS 4 TO 7 MEAN 18 7 18 12 

 S.D. 15.3 15.9 13.7 12.9 

 N 20 21 20 22 

 DAYS 7 TO 14 MEAN 20 21 24 17 

 S.D. 9.7 20.0 11.1 15.4 

 N 20 21 20 22 

 DAYS 14 TO 21 MEAN -15 -4* -15 -8 

 S.D. 12.3 9.7 14.8 16.0 

 N 20 21 20 22 

 DAYS 21 TO 28 MEAN -25 -21 -21 -17 

 S.D. 14.3 15.8 12.2 16.5 

 N 20 21 20 22 

 DAYS 1 TO 28 MEAN 5 11 13 19**

 S.D. 14.6 11.7 13.8 16.2 

 N 19 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:01

Huntingdon Life Sciences 00-4206 PAGE 71 

 

 TABLE 11 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 MEAN 153 155 149 148 

 S.D. 8.1 8.7 6.9 7.5 

 N 26 26 26 25 

 DAY 7 MEAN 117 115 116 114 

 S.D. 5.1 5.1 4.5 5.7 

 N 26 26 26 26 

 DAY 14 MEAN 103 100* 99** 98**

 S.D. 4.8 5.3 3.6 4.6 

 N 26 26 26 26 

 DAY 21 MEAN 92 89* 88** 91 

 S.D. 4.4 4.0 3.9 5.2 

 N 26 26 26 26 

 DAY 28 MEAN 79 78 79 80 

 S.D. 4.1 3.7 5.0 4.3 

 N 26 26 26 26 

 DAY 35 MEAN 74 72 71 73 

 S.D. 4.1 2.7 3.6 4.5 

 N 26 26 26 26 

 DAY 42 MEAN 68 66 66 68 

 S.D. 4.3 2.7 2.5 4.1 

 N 26 26 26 26 

 DAY 49 MEAN 64 63 63 63 

 S.D. 3.4 2.7 3.0 3.2 

 N 26 26 26 26 

 DAY 56 MEAN 62 60* 60 62 

 S.D. 3.5 2.6 2.3 2.9 

 N 26 26 26 26 

 DAY 63 MEAN 60 58 60 60 

 S.D. 3.8 2.7 2.0 2.8 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:01

Huntingdon Life Sciences 00-4206 PAGE 72 

 

 TABLE 11 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 69 MEAN 56 55 54 53 

 S.D. 3.9 3.0 2.2 10.0 

 N 26 26 26 26 

 DAY 91 MEAN 51 49 51 52 

 S.D. 3.6 2.6 2.3 3.3 

 N 26 26 26 26 

 DAY 98 MEAN 50 48 48 51 

 S.D. 3.6 2.4 2.2 4.0 

 N 26 26 26 26 

 DAY 105 MEAN 48 48 47 50 

 S.D. 4.2 2.6 2.3 3.3 

 N 26 26 26 26 

 DAY 112 MEAN 47 45 47 50* 

 S.D. 2.9 3.0 2.7 3.4 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 11:03

Huntingdon Life Sciences 00-4206 PAGE 73 

 

 TABLE 11 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 MEAN 127 130 130 133 

 S.D. 8.3 6.2 9.7 9.2 

 N 25 26 25 26 

 DAY 7 MEAN 98 101 100 97 

 S.D. 6.1 4.8 7.0 7.1 

 N 26 26 26 25 

 DAY 14 MEAN 94 95 94 93 

 S.D. 4.5 6.5 6.7 4.8 

 N 26 26 26 26 

 DAY 21 MEAN 88 91 89 89 

 S.D. 4.9 5.6 5.4 4.9 

 N 26 26 26 26 

 DAY 28 MEAN 80 85** 83 84 

 S.D. 4.6 5.4 7.2 4.0 

 N 26 26 26 26 

 DAY 35 MEAN 78 81* 79 81* 

 S.D. 5.0 5.4 4.7 4.8 

 N 26 26 26 26 

 DAY 42 MEAN 71 76** 75* 78**

 S.D. 4.4 5.4 5.7 4.6 

 N 26 26 26 25 

 DAY 49 MEAN 71 72 72 72 

 S.D. 3.5 5.4 4.8 4.4 

 N 26 26 26 25 

 DAY 56 MEAN 67 68 67 68 

 S.D. 3.3 3.8 4.4 2.8 

 N 26 26 26 25 

 DAY 63 MEAN 66 69 70 68 

 S.D. 4.4 7.5 5.9 4.7 

 N 26 26 26 25 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:03

Huntingdon Life Sciences 00-4206 PAGE 74 

 

 TABLE 11 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 69 MEAN 63 65 62 65 

 S.D. 3.6 7.1 9.8 4.6 

 N 25 25 26 25 

 DAY 91 MEAN 59 59 56 60 

 S.D. 11.2 3.5 0.9 0.4 

 N 3 3 3 2 

 DAY 98 MEAN 57 56 51 53 

 S.D. 2.0 6.7 4.5 0.0 

 N 3 3 3 1 

 DAY 105 MEAN 57 54 53 58 

 S.D. 2.6 5.7 4.4 0.0 

 N 2 4 5 1 

 DAY 112 MEAN 49 51 

 S.D. 0.0 7.8 

 N 1 2 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:04

Huntingdon Life Sciences 00-4206 PAGE 75 

 

 TABLE 12 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF GESTATION FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 0 TO 4 MEAN 74 74 74 74 

 S.D. 6.3 7.6 5.3 6.5 

 N 20 22 21 21 

 DAYS 4 TO 7 MEAN 74 76 76 76 

 S.D. 4.3 10.0 6.3 6.2 

 N 20 22 22 21 

 DAYS 7 TO 14 MEAN 70 71 71 71 

 S.D. 3.9 6.0 4.2 9.6 

 N 20 22 22 21 

 DAYS 14 TO 20 MEAN 62 62 61 65 

 S.D. 5.7 5.7 11.1 3.7 

 N 20 22 22 21 

 DAYS 0 TO 20 MEAN 70 71 70 72 

 S.D. 3.4 6.5 4.9 5.1 

 N 20 22 22 21 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:05

Huntingdon Life Sciences 00-4206 PAGE 76 

 

 TABLE 13 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF LACTATION FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 1 TO 4 MEAN 106 92* 102 100 

 S.D. 10.3 17.6 17.9 14.0 

 N 19 21 20 22 

 DAYS 4 TO 7 MEAN 115 108 113 109 

 S.D. 15.3 16.3 14.1 11.4 

 N 20 21 20 22 

 DAYS 7 TO 14 MEAN 144 128** 137 136 

 S.D. 7.7 16.8 13.0 6.8 

 N 20 20 20 22 

 DAYS 14 TO 21 MEAN 172 174 165 171 

 S.D. 14.7 23.3 18.2 12.0 

 N 20 21 20 22 

 DAYS 21 TO 28 MEAN 307 299 301 291 

 S.D. 37.0 45.1 26.1 29.0 

 N 20 21 20 22 

 DAYS 1 TO 28 MEAN 169 160 164 162 

 S.D. 12.8 19.5 13.5 9.0 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 77 

 

 TABLE 14 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ESTROUS STAGES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

PREMATING TREATMENT PERIOD (21 days evaluated)a

 

 

FEMALES EXAMINED N 26 26 26 26 

CYCLE LENGTH (days) MEAN 4.3 4.2 4.3 4.1 

 S.D. 0.56 0.43 0.80 0.49 

 N 26 24 23 25 

NUMBER OF ESTROUS CYCLES MEAN 3.8 3.4 3.2 3.8 

 S.D. 0.99 0.97 1.24 0.85 

 N 26 24 23 25 

-----------------------------------------------------------------------------------------------------------------------------------

a

Any periods of pseudopregnancy were excluded from these calculations (see also Appendix P).

No statistically significant differences 
23-JUN-2009 11:07

Huntingdon Life Sciences 00-4206 PAGE 78 

 

 TABLE 15 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF COHABITATION DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Males placed with females N 26 26 26 25 

 Total number mated N 24 23 23 24 

 male mating index % 92.3 88.5 88.5 96.0 

 with females pregnant N 21 22 22 22 

 male fertility index % 80.8 84.6 84.6 88.0 

Females with defined

 day 0 of Gestation N 23 23 23 23 

No. of days until Mating MEAN 2.7 3.0 3.0 4.0 

 S.D. 1.60 1.69 1.77 2.70 

 Day 1 to 4 N 21 21 18 14 

 % 91.3 91.3 78.3 60.9 

 Day 5 to 8 N 2 1 5 8 

 % 8.7 4.3 21.7 34.8 

 Day 9 to 14 N 0 1 0 1 

 % 0.0 4.3 0.0 4.3 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:10

Huntingdon Life Sciences 00-4206 PAGE 79 

 

 TABLE 16 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Females on Study N 26 26 26 26 

Females Mated N 24 23 23 24 

 Mating Index % 92.3 88.5 88.5 92.3 

Females Pregnant N 21 22 22 22 

 Female Fertility Index % 87.5 95.7 95.7 91.7 

Females with Liveborn N 21 22 22 22 

 Gestation Index % 100.0 100.0 100.0 100.0 

Females Completing Delivery N 21 22 22 22 

 % 100.0 100.0 100.0 100.0 

 with Stillborn Pups N 1 5 5 0 

 % 4.8 22.7 22.7 0.0 

 with all Stillborn N 0 0 0 0 

 % 0.0 0.0 0.0 0.0 

 Litters with Liveborn, 

 but no Pups Alive

 day 4 N 0 1 1 0 

 % 0.0 4.5 4.5 0.0 

 day 21 N 0 1 1 0 

 % 0.0 4.5 4.5 0.0 

Duration of Gestation MEAN 22.0 22.2 22.0 22.0 

 S.D. 0.00 0.39 0.58 0.45 

 N 20 22 22 21 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:10

Huntingdon Life Sciences 00-4206 PAGE 80 

 

 TABLE 16 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters with Liveborn Pups N 21 22 22 22 

Pups Delivered (total) N 300 264 303 310 

 MEAN 14.3 12.0* 13.8 14.1 

 S.D. 1.23 3.83 2.45 2.09 

 Liveborn N 299 259 291** 310 

 Live Birth Index % 99.7 98.1 96.0 100.0 

 Stillborn N 1 5 12** 0 

 % 0.3 1.9 4.0 0.0 

 Culled day 4 84 54 76 78 

 Liveborn, not culled N 215 205 215 232 

 prior to day 21

Pups Dying, Missing, and/or Cannibalized

 day 0 N 0 0 0 0 

 % 0.0 0.0 0.0 0.0 

 days 1-4 N 1 4 18** 12**

 % 0.3 1.5 6.2 3.9 

 days 5-21 N 1 2 0 1 

 % 0.3 0.8 0.0 0.3 

 days 0-4 N 1 4 18** 12**

 % 0.3 1.5 6.2 3.9 

 days 0-21 N 2 6 18** 13* 

 % 0.7 2.3 6.2 4.2 

Pups Surviving 4 days N 286 255 273 298 

 Viability Index % 95.7 98.5 93.8 96.1 

Pups Surviving 21 days N 201 199 197 219 

 Lactation Index % 99.5 99.0 100.0 99.5 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:10

Huntingdon Life Sciences 00-4206 PAGE 81 

 

 TABLE 16 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Implantation Sites N 318 296 336 336 

 per Litter MEAN 15.1 14.1 15.3 15.3 

 S.D. 1.53 2.43 1.32 1.16 

Sex Ratio - Male Pups:Total Pups

 day 0 N 153 118 143 159 

 % 51.2 45.6 49.1 51.3 

 day 21 N 104 93 100 113 

 % 51.7 46.7 50.8 51.6 

Live Pups/Litter

 day 1 MEAN 14.2 11.7* 13.2 13.8 

 S.D. 1.22 3.85 3.51 2.16 

 N 21 22 21 22 

 day 4 preculling MEAN 13.6 12.1 13.0 13.5 

 S.D. 2.97 3.05 3.55 2.04 

 N 21 21 21 22 

 day 4 postculling MEAN 9.6 9.6 9.4 10.0 

 S.D. 1.75 1.66 1.91 0.31 

 N 21 21 21 22 

 day 7 MEAN 9.6 9.6 9.4 10.0 

 S.D. 1.75 1.66 1.91 0.38 

 N 21 21 21 22 

 day 14 MEAN 9.6 9.5 9.4 10.0 

 S.D. 1.75 1.63 1.91 0.38 

 N 21 21 21 22 

 day 21 MEAN 9.6 9.5 9.4 10.0 

 S.D. 1.75 1.63 1.91 0.38 

 N 21 21 21 22 

 day 28 MEAN 9.6 9.5 9.4 10.0 

 S.D. 1.75 1.63 1.91 0.38 

 N 21 21 21 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 11:10

Huntingdon Life Sciences 00-4206 PAGE 82 

 

 TABLE 16 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Pup Weight/Litter (grams)

 day 1 MEAN 6.9 7.5* 6.9 7.0 

 S.D. 0.56 0.73 0.71 0.64 

 N 21 22 20 22 

 day 4 MEAN 9.9 11.0* 10.0 9.9 

 S.D. 1.15 1.39 1.58 1.02 

 N 20 21 20 22 

 day 7 MEAN 13.9 14.7 13.9 13.1 

 S.D. 1.85 1.44 2.14 1.30 

 N 20 21 20 22 

 day 14 MEAN 25.1 24.6 24.3 22.7* 

 S.D. 3.17 2.64 2.86 2.09 

 N 20 21 20 22 

 day 21 MEAN 40.2 41.3 40.6 37.9 

 S.D. 5.32 4.40 6.03 3.85 

 N 20 21 20 22 

 day 28 MEAN 76.3 77.6 76.5 71.9 

 S.D. 8.39 6.14 9.45 5.30 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 11:13

Huntingdon Life Sciences 00-4206 PAGE 83 

 

 TABLE 17 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP CLINICAL OBSERVATIONS DURING LACTATION - (frequency/animals) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 1 to 28

 Normal 

----------

WITHIN NORMAL LIMITS 1081/20 1038/21 1046/20 1169/22 
23-JUN-2009 11:16

Huntingdon Life Sciences 00-4206 PAGE 84 

 

 TABLE 18 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 day 1 males MEAN 7.2 7.8* 7.1 7.2 

 S.D. 0.52 0.74 0.68 0.69 

 N 20 21 20 22 

 1 females MEAN 6.7 7.3** 6.6 6.7 

 S.D. 0.52 0.72 0.50 0.64 

 N 20 22 19 22 

 1 males+females MEAN 7.0 7.5* 6.9 7.0 

 S.D. 0.50 0.73 0.71 0.64 

 N 20 22 20 22 

 day 4 males MEAN 10.2 11.3* 10.3 10.1 

 preculling S.D. 1.16 1.38 1.57 1.09 

 N 20 21 20 22 

 4 females MEAN 9.6 10.8** 9.5 9.6 

 preculling S.D. 1.19 1.44 0.77 1.03 

 N 20 21 19 22 

 4 males+females MEAN 9.9 11.0* 10.0 9.9 

 preculling S.D. 1.15 1.39 1.58 1.02 

 N 20 21 20 22 

 day 4 males MEAN 10.2 11.3* 10.3 10.1 

 postculling S.D. 1.20 1.41 1.57 1.12 

 N 20 21 20 22 

 4 females MEAN 9.6 10.8** 9.5 9.6 

 postculling S.D. 1.21 1.44 0.75 1.02 

 N 20 21 19 22 

 4 males+females MEAN 9.9 11.0* 10.0 9.9 

 postculling S.D. 1.18 1.40 1.58 1.04 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:16

Huntingdon Life Sciences 00-4206 PAGE 85 

 

 TABLE 18 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 day 7 males MEAN 14.3 15.0 14.2 13.5 

 S.D. 2.03 1.39 2.12 1.38 

 N 20 21 20 22 

 7 females MEAN 13.5 14.5 13.2 12.8 

 S.D. 1.74 1.61 1.24 1.32 

 N 20 21 19 22 

 7 males+females MEAN 13.9 14.7 13.9 13.1 

 S.D. 1.85 1.44 2.14 1.30 

 N 20 21 20 22 

 day 14 males MEAN 25.5 25.0 24.7 23.1* 

 S.D. 3.39 2.65 2.84 2.12 

 N 20 21 20 22 

 14 females MEAN 24.6 24.2 23.4 22.3* 

 S.D. 3.03 2.74 1.88 2.17 

 N 20 21 19 22 

 14 males+females MEAN 25.1 24.6 24.3 22.7* 

 S.D. 3.17 2.64 2.86 2.09 

 N 20 21 20 22 

 day 21 males MEAN 40.8 41.9 41.1 38.2 

 S.D. 6.09 4.52 6.12 3.86 

 N 20 21 20 22 

 21 females MEAN 39.6 40.7 39.2 37.5 

 S.D. 4.75 4.46 4.37 4.03 

 N 20 21 19 22 

 21 males+females MEAN 40.2 41.3 40.6 37.9 

 S.D. 5.32 4.40 6.03 3.85 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 11:16

Huntingdon Life Sciences 00-4206 PAGE 86 

 

 TABLE 18 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 day 28 males MEAN 79.3 81.0 79.1 73.9 

 S.D. 10.13 6.83 9.35 5.45 

 N 20 21 20 22 

 28 females MEAN 73.0 74.5 72.1 69.7 

 S.D. 7.04 5.79 6.27 5.56 

 N 20 21 19 22 

 28 males+females MEAN 76.3 77.6 76.5 71.9 

 S.D. 8.39 6.14 9.45 5.30 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:16

Huntingdon Life Sciences 00-4206 PAGE 87 

 

 TABLE 18 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP BODY WEIGHT GAIN -- GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

day 1- 4 males MEAN 3.0 3.5 3.2 2.9 

 S.D. 0.77 0.82 0.97 0.66 

 N 20 21 20 22 

 females MEAN 2.9 3.4 2.9 2.9 

 S.D. 0.80 0.89 0.40 0.63 

 N 20 21 19 22 

 males+females MEAN 3.0 3.4 3.1 2.9 

 S.D. 0.77 0.85 0.96 0.62 

 N 20 21 20 22 

day 4- 7 males MEAN 4.1 3.8 4.0 3.4* 

 S.D. 0.97 0.77 0.78 0.65 

 N 20 21 20 22 

 females MEAN 3.9 3.7 3.7 3.2**

 S.D. 0.77 0.78 0.69 0.69 

 N 20 21 19 22 

 males+females MEAN 4.0 3.7 3.9 3.3**

 S.D. 0.85 0.74 0.77 0.66 

 N 20 21 20 22 

day 4-21 males MEAN 30.6 30.6 30.8 28.1 

 S.D. 5.24 4.04 4.86 3.57 

 N 20 21 20 22 

 females MEAN 30.0 29.9 29.7 27.9 

 S.D. 4.11 3.75 3.82 3.68 

 N 20 21 19 22 

 males+females MEAN 30.3 30.3 30.6 28.0 

 S.D. 4.59 3.80 4.71 3.54 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:16

Huntingdon Life Sciences 00-4206 PAGE 88 

 

 TABLE 18 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP BODY WEIGHT GAIN -- GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

day 4-28 males MEAN 69.2 69.8 68.9 63.8 

 S.D. 9.25 6.43 8.10 5.11 

 N 20 21 20 22 

 females MEAN 63.4 63.7 62.6 60.1 

 S.D. 6.29 5.18 5.80 5.11 

 N 20 21 19 22 

 males+females MEAN 66.4 66.6 66.4 62.1 

 S.D. 7.61 5.64 8.13 4.93 

 N 20 21 20 22 

day 7-14 males MEAN 11.2 10.0 10.4 9.6**

 S.D. 1.74 2.10 1.09 1.47 

 N 20 21 20 22 

 females MEAN 11.1 9.7* 10.3 9.5**

 S.D. 1.91 1.70 0.95 1.47 

 N 20 21 19 22 

 males+females MEAN 11.2 9.9* 10.4 9.6**

 S.D. 1.81 1.84 1.01 1.45 

 N 20 21 20 22 

day 7-28 males MEAN 65.1 66.0 64.9 60.4 

 S.D. 8.37 6.02 7.62 4.76 

 N 20 21 20 22 

 females MEAN 59.5 60.0 58.9 56.9 

 S.D. 5.63 4.62 5.43 4.67 

 N 20 21 19 22 

 males+females MEAN 62.4 62.9 62.6 58.8 

 S.D. 6.84 5.18 7.62 4.54 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 11:16

Huntingdon Life Sciences 00-4206 PAGE 89 

 

 TABLE 18 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP BODY WEIGHT GAIN -- GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

day 14-21 males MEAN 15.3 16.9 16.4 15.1 

 S.D. 3.00 2.33 3.49 2.06 

 N 20 21 20 22 

 females MEAN 15.0 16.5 15.8 15.2 

 S.D. 2.20 2.05 2.61 2.02 

 N 20 21 19 22 

 males+females MEAN 15.2 16.7 16.3 15.2 

 S.D. 2.49 2.09 3.31 1.96 

 N 20 21 20 22 

day 14-28 males MEAN 53.9 56.0 54.5 50.9 

 S.D. 6.98 4.43 6.80 3.72 

 N 20 21 20 22 

 females MEAN 48.4 50.3 48.7 47.4 

 S.D. 4.44 3.28 4.60 3.60 

 N 20 21 19 22 

 males+females MEAN 51.3 53.0 52.2 49.2 

 S.D. 5.52 3.71 6.75 3.49 

 N 20 21 20 22 

day 21-28 males MEAN 38.6 39.2 38.1 35.8* 

 S.D. 4.56 2.85 3.79 2.04 

 N 20 21 20 22 

 females MEAN 33.4 33.8 32.9 32.2 

 S.D. 3.00 1.88 2.65 1.92 

 N 20 21 19 22 

 males+females MEAN 36.1 36.3 35.8 34.1 

 S.D. 3.60 2.27 3.86 1.93 

 N 20 21 20 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
23-JUN-2009 11:22

Huntingdon Life Sciences 00-4206 PAGE 90 

 

 TABLE 19 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26 

THYMUS N 1 3 4 4 

------

DISCOLORED FOCI N 1 3 4 4 

 % 3.8 11.5 15.4 15.4 

LUNGS N 4 8 10 12 

-----

 DISCOLORED FOCI N 4 8 10 12 

 % 15.4 30.8 38.5 46.2 

ABDOMINAL CAVITY N 1 0 0 0 

----------------

DISCOLORED LYMPH NODE(S) N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

KIDNEY N 3 3 5 4 

------

 DILATED RENAL PELVIS N 2 3 5 4 

 % 7.7 11.5 19.2 15.4 

IRREGULAR SURFACE N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:22

Huntingdon Life Sciences 00-4206 PAGE 91 

 

 TABLE 19 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26 

BLADDER N 0 1 0 0 

-------

CALCULI N 0 1 0 0 

 % 0.0 3.8 0.0 0.0 

GONADS N 1 0 0 1 

------

TESTE(S) SMALL N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

TESTE(S) SOFT N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

EPIDIDYMIS(IDES) SMALL N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

 UNDESCENDED TESTE(S) N 0 0 0 1 

 % 0.0 0.0 0.0 3.8 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:22

Huntingdon Life Sciences 00-4206 PAGE 92 

 

 TABLE 19 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALES N 26 26 26 26 

THYMUS N 1 0 0 1 

------

DISCOLORED FOCI N 1 0 0 1 

 % 3.8 0.0 0.0 3.8 

LUNGS N 2 9 4 14 

-----

 DISCOLORED FOCI N 2 9 4 14**

 % 7.7 34.6 15.4 53.8 

ABDOMINAL CAVITY N 0 1 0 0 

----------------

MASS N 0 1 0 0 

 % 0.0 3.8 0.0 0.0 

LIVER N 2 0 0 0 

-----

ADHESION N 2 0 0 0 

 % 7.7 0.0 0.0 0.0 

MASS N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
23-JUN-2009 11:22

Huntingdon Life Sciences 00-4206 PAGE 93 

 

 TABLE 19 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALES N 26 26 26 26 

KIDNEY N 1 0 0 1 

------

 DILATED RENAL PELVIS N 0 0 0 1 

 % 0.0 0.0 0.0 3.8 

MASS N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

ADRENALS N 1 1 1 0 

--------

 DISCOLORED ADRENALS N 1 1 0 0 

 % 3.8 3.8 0.0 0.0 

DISCOLORED FOCI N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

GONADS N 1 0 0 0 

------

FLUID-FILLED VAGINA N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

UTERUS N 1 0 0 0 

------

FETUS(ES) IN UTERUS N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 11:22

Huntingdon Life Sciences 00-4206 PAGE 94 

 

 TABLE 19 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

----------------------------------------------------------------------------------------------------------------------------------- 

FEMALES N 26 26 26 26 

OVARY N 1 0 0 0 

-----

CYST(S) N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
23-JUN-2009 12:49

Huntingdon Life Sciences 00-4206 PAGE 95 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

SUMMARY OF NECROPSY OBSERVATIONS FOR DEAD PUPS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 2 4 4 5 

Pups Evaluated N 2 4 6 11 

GROSS EXAM 

----------

 Litter Incidence N 1 0 0 2 

 Pup Incidence N 1 0 0 3 

AUTOLYSIS 

 Pup Incidence N 1 0 0 3 

 % 50.0 0.0 0.0 27.3 

 Litter Incidence N 1 0 0 2 

 % 50.0 0.0 0.0 40.0 

CANNIBALIZED 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 9.1 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 20.0 

LUNGS 

-----

 Litter Incidence N 0 3 2 2 

 Pup Incidence N 0 3 3 6 

LUNG FLOATATION TEST - FOUND DEAD 

 Pup Incidence N 0 3 3 6 

 % 0.0 75.0 50.0 54.5 

 Litter Incidence N 0 3 2 2 

 % 0.0 75.0 50.0 40.0 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 12:49

Huntingdon Life Sciences 00-4206 PAGE 96 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

SUMMARY OF NECROPSY OBSERVATIONS FOR DEAD PUPS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 2 4 4 5 

Pups Evaluated N 2 4 6 11 

STOMACH 

------- 

 Litter Incidence N 1 4 4 4 

 Pup Incidence N 1 4 6 8 

MILK IN STOMACH 

 Pup Incidence N 1 2 3 0 

 % 50.0 50.0 50.0 0.0 

 Litter Incidence N 1 2 3 0 

 % 50.0 50.0 75.0 0.0 

 NO MILK IN STOMACH 

 Pup Incidence N 0 2 3 8 

 % 0.0 50.0 50.0 72.7 

 Litter Incidence N 0 2 2 4 

 % 0.0 50.0 50.0 80.0 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 12:51

Huntingdon Life Sciences 00-4206 PAGE 97 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF NECROPSY OBSERVATIONS FOR STILLBORN PUPSa

 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 1 5 5 0 

Pups Evaluated N 1 5 12 0 

GROSS EXAM 

----------

 Litter Incidence N 0 1 2 0 

 Pup Incidence N 0 1 2 0 

AUTOLYSIS 

 Pup Incidence N 0 1 2 

 % 0.0 20.0 16.7 

 Litter Incidence N 0 1 2 

 % 0.0 20.0 40.0 

CANNIBALIZED 

 Pup Incidence N 0 1 2 

 % 0.0 20.0 16.7 

 Litter Incidence N 0 1 1 

 % 0.0 20.0 20.0 

SEX UNDETERMINED 

 Pup Incidence N 0 1 2 

 % 0.0 20.0 16.7 

 Litter Incidence N 0 1 1 

 % 0.0 20.0 20.0 

LUNGS 

-----

 Litter Incidence N 1 3 4 0 

 Pup Incidence N 1 3 8 0 

LUNG FLOATATION TEST - STILLBORN 

 Pup Incidence N 1 3 8 

 % 100.0 60.0 66.7 

 Litter Incidence N 1 3 4 

 % 100.0 60.0 80.0 

-----------------------------------------------------------------------------------------------------------------------------------

a

Includes autolytic or cannibalized pups for which a lung floatation test could not be performed.
23-JUN-2009 12:51

Huntingdon Life Sciences 00-4206 PAGE 98 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF NECROPSY OBSERVATIONS FOR STILLBORN PUPSa

 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 1 5 5 0 

Pups Evaluated N 1 5 12 0 

STOMACH 

-------

 Litter Incidence N 1 3 4 0 

 Pup Incidence N 1 3 8 0 

 NO MILK IN STOMACH 

 Pup Incidence N 1 3 8 

 % 100.0 60.0 66.7 

 Litter Incidence N 1 3 4 

 % 100.0 60.0 80.0 

-----------------------------------------------------------------------------------------------------------------------------------

a

Includes autolytic or cannibalized pups for which a lung floatation test could not be performed.
23-JUN-2009 12:53

Huntingdon Life Sciences 00-4206 PAGE 99 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF NECROPSY OBSERVATIONS FOR CULLED PUPS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 20 17 18 20 

Pups Evaluated N 84 54 76 78 

TOTAL PUPS CULLED OBSERVATIONS 

 N 0 0 0 0 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 12:55

Huntingdon Life Sciences 00-4206 PAGE 100 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF NECROPSY OBSERVATIONS FOR CANNIBALIZED PUPS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 0 0 2 0 

Pups Evaluated N 0 0 6 0 

GROSS EXAM 

----------

 Litter Incidence N 0 0 2 0 

 Pup Incidence N 0 0 6 0 

CANNIBALIZED 

 Pup Incidence N 6 

 % 100.0 

 Litter Incidence N 2 

 % 100.0 

SEX UNDETERMINED 

 Pup Incidence N 6 

 % 100.0 

 Litter Incidence N 2 

 % 100.0 

STOMACH 

-------

 Litter Incidence N 0 0 1 0 

 Pup Incidence N 0 0 5 0 

MILK IN STOMACH UNDETERMINED 

 Pup Incidence N 5 

 % 83.3 

 Litter Incidence N 1 

 % 50.0 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 12:56

Huntingdon Life Sciences 00-4206 PAGE 101 

 

 TABLE 20 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMARY OF NECROPSY OBSERVATIONS FOR WEANLING PUPS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 20 21 20 22 

Pups Evaluated N 120 123 117 132 

KIDNEY 

------

 Litter Incidence N 1 0 4 2 

 Pup Incidence N 1 0 5 2 

DILATED RENAL PELVIS (PAPILLA REDUCED) 

 Pup Incidence N 1 0 5 2 

 % 0.8 0.0 4.3 1.5 

 Litter Incidence N 1 0 4 2 

 % 5.0 0.0 20.0 9.1 

GONADS 

------

 Litter Incidence N 2 4 2 1 

 Pup Incidence N 2 4 3 1 

UNDESCENDED TESTE(S) 

 Pup Incidence N 2 3 3 1 

 % 1.7 2.4 2.6 0.8 

 Litter Incidence N 2 3 2 1 

 % 10.0 14.3 10.0 4.5 

SMALL TESTE(S) 

 Pup Incidence N 0 1 0 0 

 % 0.0 0.8 0.0 0.0 

 Litter Incidence N 0 1 0 0 

 % 0.0 4.8 0.0 0.0 

SMALL EPIDIDYMIS(IDES) 

 Pup Incidence N 0 1 0 0 

 % 0.0 0.8 0.0 0.0 

 Litter Incidence N 0 1 0 0 

 % 0.0 4.8 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------
Huntingdon Life Sciences 00-4206 Page 102 

211-TBA-1G Final Report 

Summary of Parental Organ Weights 

Preface Table 21 

Table of Contents 

Summary of absolute organ weights ................................................................................103 

Summary of organ to body weight ratio ..........................................................................107 

Summary of organ to brain weight ratio ..........................................................................111 

Key to Abbreviations: 

g = Grams 

SEM. VES & CO. GL. = Seminal Vesicles and Coagulating Gland 

Corresponding exposure levels for each group were as follows: 

Group I - 0 mg/m3

Group II - 2000 mg/m3

Group III - 10000 mg/m3

Group IV - 20000 mg/m3
23-JUN-2009 13:04 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ABSOLUTE ORGAN WEIGHTS MALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 569 576 556  S.D. 57.0 41.8 52.2 3 N 26 26 26 TESTIS - RIGHT g MEAN 1.7925 1.7901 1.7888 1.7 S.D. .13517 .15635 .11505 .16 N 26 26 26 TESTIS - LEFT g MEAN 1.7624 1.7816 1.7859 1.7 S.D. .21606 .14279 .10812 .16 N 26 26 26 TESTES g MEAN 3.5384 3.5596 3.5631 3.5 S.D. .31536 .29683 .21703 .32 N 26 26 26 EPIDIDYMIS RIGHT g MEAN 0.7381 0.7569 0.7505 0.7 S.D. .06263 .06071 .07239 .05 N 26 26 26 EPIDIDYMIS LEFT g MEAN 0.7234 0.7505 0.7388 0.6 S.D. .07847 .06446 .07537 .05 N 26 26 25 EPIDIDYMIDES g MEAN 1.4520 1.5015 1.4805 1.3 S.D. .12840 .12046 .14157 .09 N 26 26 25 PROSTATE g MEAN 1.0457 1.1186 1.0995 1.1 S.D. .16987 .18424 .11776 .22 N 26 26 26 SEM.VES & CO.GL. g MEAN 2.2416 2.0959 2.3462 2.1 S.D. .31168 .27162 .33621 .35 N 26 26 26 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 
23-JUN-2009 13:04 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ABSOLUTE ORGAN WEIGHTS MALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------ADRENALS g MEAN 0.0663 0.0656 0.0691 0.0 S.D. .00764 .01163 .01139 .01 N 26 26 26 BRAIN g MEAN 2.1416 2.1758 2.1519 2.1 S.D. .09677 .09573 .08358 .08 N 26 26 26 KIDNEYS g MEAN 4.1621 4.3807 4.8115** 4.8 S.D. .46126 .46640 .46759 .44 N 26 26 26 LIVER g MEAN 20.116 20.907 20.804 21. S.D. 2.2338 2.4937 2.6955 2.8 N 26 26 26 LUNGS g MEAN 1.9724 2.0157 1.8955 1.8 S.D. .21622 .23339 .18587 .17 N 26 26 26 PITUITARY g MEAN 0.0126 0.0129 0.0120 0.0 S.D. .00170 .00167 .00261 .00 N 26 26 26 SPLEEN g MEAN 0.8980 0.8472 0.8148* 0.7 S.D. .14468 .11408 .12958 .08 N 26 26 26 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ABSOLUTE ORGAN WEIGHTS FEMALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 330 329 329  S.D. 23.3 25.6 18.6 2 N 20 21 20 OVARY RIGHT g MEAN 0.0624 0.0614 0.0640 0.0 S.D. .01422 .01367 .01260 .01 N 20 21 20 OVARY LEFT g MEAN 0.0628 0.0552 0.0604 0.0 S.D. .02558 .01187 .01423 .01 N 20 21 20 OVARIES g MEAN 0.1229 0.1152 0.1238 0.1 S.D. .03215 .02283 .02447 .01 N 20 21 20 UTERUS/OVIDUCTS g MEAN 0.7183 0.6427 0.6228 0.6 S.D. .19003 .19923 .19070 .22 N 20 21 20 ADRENALS g MEAN 0.0832 0.0801 0.0809 0.1 S.D. .00741 .01310 .01201 .10 N 20 21 20 BRAIN g MEAN 1.9762 1.9704 1.9640 1.9 S.D. .12457 .07731 .07489 .08 N 20 21 20 KIDNEYS g MEAN 3.3461 2.8058 2.8555 2.9 S.D. 2.6250 .32166 .15930 .24 N 20 21 20 ------------------------------------------------------------------------------------------------------------------No statistically significant differences 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ABSOLUTE ORGAN WEIGHTS FEMALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------LIVER g MEAN 15.244 15.752 15.573 16. S.D. 1.7866 2.2077 1.6969 1.9 N 20 21 20 LUNGS g MEAN 1.5451 1.5705 1.5477 1.5 S.D. .17124 .13311 .11356 .11 N 20 21 20 PITUITARY g MEAN 0.0141 0.0150 0.0130 0.0 S.D. .00241 .00297 .00389 .00 N 20 21 20 SPLEEN g MEAN 0.7124 0.6256* 0.6363* 0.6 S.D. .10949 .10738 .09522 .07 N 20 21 20 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO MALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 569 576 556  S.D. 57.0 41.8 52.2 3 N 26 26 26 TESTIS - RIGHT Ratio MEAN 0.0032 0.0031 0.0032 0.0 S.D. .00029 .00028 .00040 .00 N 26 26 26 TESTIS - LEFT Ratio MEAN 0.0031 0.0031 0.0032 0.0 S.D. .00043 .00026 .00040 .00 N 26 26 26 TESTES Ratio MEAN 0.0063 0.0062 0.0065 0.0 S.D. .00067 .00054 .00079 .00 N 26 26 26 EPIDIDYMIS RIGHT Ratio MEAN 0.0013 0.0013 0.0014 0.0 S.D. .00012 .00011 .00016 .00 N 26 26 26 EPIDIDYMIS LEFT Ratio MEAN 0.0013 0.0013 0.0013 0.0 S.D. .00015 .00010 .00014 .00 N 26 26 25 EPIDIDYMIDES Ratio MEAN 0.0026 0.0026 0.0027 0.0 S.D. .00025 .00021 .00030 .00 N 26 26 25 PROSTATE Ratio MEAN 0.0018 0.0019 0.0020 0.0 S.D. .00027 .00032 .00021 .00 N 26 26 26 SEM.VES & CO.GL. Ratio MEAN 0.0040 0.0037 0.0042 0.0 S.D. .00064 .00052 .00047 .00 N 26 26 26 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO MALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------ADRENALS Ratio MEAN 0.0001 0.0001 0.0001 0.0 S.D. .00002 .00002 .00002 .00 N 26 26 26 BRAIN Ratio MEAN 0.0038 0.0038 0.0039 0.0 S.D. .00031 .00028 .00036 .00 N 26 26 26 KIDNEYS Ratio MEAN 0.0073 0.0076 0.0087** 0.0 S.D. .00051 .00070 .00060 .00 N 26 26 26 LIVER Ratio MEAN 0.0354 0.0363 0.0374 0.0 S.D. .00277 .00301 .00279 .00 N 26 26 26 LUNGS Ratio MEAN 0.0035 0.0035 0.0034 0.0 S.D. .00028 .00041 .00031 .00 N 26 26 26 PITUITARY Ratio MEAN 0.0000 0.0000 0.0000 0.0 S.D. .00000 .00000 .00000 .00 N 26 26 26 SPLEEN Ratio MEAN 0.0016 0.0015 0.0015 0.0 S.D. .00019 .00018 .00019 .00 N 26 26 26 ------------------------------------------------------------------------------------------------------------------Statistical key: ** = p<0.01 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO FEMALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 330 329 329  S.D. 23.3 25.6 18.6 2 N 20 21 20 OVARY RIGHT Ratio MEAN 0.0002 0.0002 0.0002 0.0 S.D. .00005 .00004 .00004 .00 N 20 21 20 OVARY LEFT Ratio MEAN 0.0002 0.0002 0.0002 0.0 S.D. .00007 .00004 .00004 .00 N 20 21 20 OVARIES Ratio MEAN 0.0004 0.0004 0.0004 0.0 S.D. .00010 .00007 .00007 .00 N 20 21 20 UTERUS/OVIDUCTS Ratio MEAN 0.0022 0.0020 0.0019 0.0 S.D. .00063 .00066 .00066 .00 N 20 21 20 ADRENALS Ratio MEAN 0.0003 0.0002 0.0002 0.0 S.D. .00003 .00004 .00004 .00 N 20 21 20 BRAIN Ratio MEAN 0.0060 0.0060 0.0060 0.0 S.D. .00031 .00045 .00031 .00 N 20 21 20 KIDNEYS Ratio MEAN 0.0101 0.0085 0.0087 0.0 S.D. .00746 .00077 .00061 .00 N 20 21 20 ------------------------------------------------------------------------------------------------------------------No statistically significant differences 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO FEMALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------LIVER Ratio MEAN 0.0461 0.0477 0.0473 0.0 S.D. .00326 .00449 .00388 .00 N 20 21 20 LUNGS Ratio MEAN 0.0047 0.0048 0.0047 0.0 S.D. .00047 .00034 .00027 .00 N 20 21 20 PITUITARY Ratio MEAN 0.0000 0.0000 0.0000 0.0 S.D. .00001 .00001 .00001 .00 N 20 21 20 SPLEEN Ratio MEAN 0.0022 0.0019** 0.0019* 0.0 S.D. .00030 .00024 .00024 .00 N 20 21 20 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO MALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------TESTIS - RIGHT Ratio MEAN 0.8373 0.8231 0.8315 0.8 S.D. .05536 .06740 .04600 .08 N 26 26 26 TESTIS - LEFT Ratio MEAN 0.8233 0.8194 0.8303 0.8 S.D. .09647 .06319 .04547 .08 N 26 26 26 TESTES Ratio MEAN 1.6529 1.6370 1.6564 1.6 S.D. .13469 .12900 .08857 .17 N 26 26 26 EPIDIDYMIS RIGHT Ratio MEAN 0.3448 0.3479 0.3487 0.3 S.D. .02614 .02341 .02959 .02 N 26 26 26 EPIDIDYMIS LEFT Ratio MEAN 0.3379 0.3451 0.3432 0.3 S.D. .03330 .02756 .03074 .02 N 26 26 25 EPIDIDYMIDES Ratio MEAN 0.6782 0.6904 0.6877 0.6 S.D. .05291 .05007 .05684 .04 N 26 26 25 PROSTATE Ratio MEAN 0.4878 0.5141 0.5119 0.5 S.D. .07167 .08093 .06030 .11 N 26 26 26 SEM.VES & CO.GL. Ratio MEAN 1.0491 0.9629 1.0922 1.0 S.D. .15495 .11441 .16551 .18 N 26 26 26 ------------------------------------------------------------------------------------------------------------------No statistically significant differences 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO MALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------ADRENALS Ratio MEAN 0.0310 0.0301 0.0321 0.0 S.D. .00329 .00508 .00482 .00 N 26 26 26 KIDNEYS Ratio MEAN 1.9417 2.0134 2.2378** 2.3 S.D. .17306 .19453 .22671 .23 N 26 26 26 LIVER Ratio MEAN 9.3948 9.6069 9.6641 10. S.D. .97181 1.0286 1.1604 1.2 N 26 26 26 LUNGS Ratio MEAN 0.9201 0.9259 0.8800 0.8 S.D. .08183 .09174 .06760 .09 N 26 26 26 PITUITARY Ratio MEAN 0.0059 0.0059 0.0056 0.0 S.D. .00066 .00085 .00126 .00 N 26 26 26 SPLEEN Ratio MEAN 0.4184 0.3892 0.3784* 0.3 S.D. .05797 .04791 .05577 .04 N 26 26 26 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO FEMALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------OVARY RIGHT Ratio MEAN 0.0316 0.0312 0.0326 0.0 S.D. .00726 .00689 .00613 .00 N 20 21 20 OVARY LEFT Ratio MEAN 0.0317 0.0280 0.0307 0.0 S.D. .01216 .00595 .00687 .00 N 20 21 20 OVARIES Ratio MEAN 0.0622 0.0585 0.0630 0.0 S.D. .01537 .01124 .01170 .00 N 20 21 20 UTERUS/OVIDUCTS Ratio MEAN 0.3670 0.3260 0.3169 0.3 S.D. .10933 .09837 .09618 .11 N 20 21 20 ADRENALS Ratio MEAN 0.0423 0.0407 0.0412 0.0 S.D. .00500 .00649 .00594 .05 N 20 21 20 KIDNEYS Ratio MEAN 1.6996 1.4245 1.4549 1.4 S.D. 1.3591 .15917 .08203 .13 N 20 21 20 LIVER Ratio MEAN 7.7172 8.0030 7.9290 8.3 S.D. .79859 1.1485 .81625 1.0 N 20 21 20 LUNGS Ratio MEAN 0.7809 0.7983 0.7886 0.7 S.D. .06068 .07763 .05766 .05 N 20 21 20 ------------------------------------------------------------------------------------------------------------------No statistically significant differences 
23-JUN-2009 13:06 

Huntingdon Life Sciences 00-4206  TABLE 21  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO FEMALES ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------PITUITARY Ratio MEAN 0.0072 0.0076 0.0067 0.0 S.D. .00141 .00155 .00203 .00 N 20 21 20 SPLEEN Ratio MEAN 0.3605 0.3180* 0.3238 0.3 S.D. .05207 .05668 .04550 .04 N 20 21 20 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
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Summary of Pup Organ Weight Data 

Preface Table 22 

Table of Contents 

Pup Organ Weights in Grams ..........................................................................................116 

Pup Organ Weight to Body Weight Ratio .......................................................................117 

Pup Organ Weight to Brain Weight Ratio .......................................................................118 
23-JUN-2009 14:13 

Huntingdon Life Sciences 00-4206  TABLE 22  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF PUP ORGAN WEIGHT DATA  PUP ORGAN WEIGHTS IN GRAMS ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------BRAIN MEAN 1.5335 1.5409 1.5486 1.5 S.D. .08286 .06367 .07232 .05 N 20 21 20  of Male Pups MEAN 1.5485 1.5762 1.5718 1.5 S.D. .09903 .06840 .08513 .05 N 20 21 20  of Female Pups MEAN 1.5185 1.5056 1.5175 1.4 S.D. .08048 .07767 .06365 .07 N 20 21 19 SPLEEN MEAN 0.3202 0.3194 0.3199 0.2 S.D. .03742 .03289 .05166 .03 N 20 21 20  of Male Pups MEAN 0.3380 0.3348 0.3333 0.2 S.D. .05149 .04731 .05394 .04 N 20 21 20  of Female Pups MEAN 0.3023 0.3041 0.2985 0.2 S.D. .04396 .03927 .04979 .03 N 20 21 19 THYMUS MEAN 0.3527 0.3487 0.3749 0.3 S.D. .05634 .03975 .06559 .04 N 20 21 20  of Male Pups MEAN 0.3570 0.3356 0.3718 0.3 S.D. .06910 .05711 .06407 .04 N 20 21 20  of Female Pups MEAN 0.3484 0.3618 0.3682 0.3 S.D. .05876 .04392 .06318 .06 N 20 21 19 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
23-JUN-2009 14:13 

Huntingdon Life Sciences 00-4206  TABLE 22  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF PUP ORGAN WEIGHT DATA  PUP ORGAN WEIGHT TO BODY WEIGHT RATIO ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------BRAIN MEAN 2.0117 1.9891 2.0493 2.1 S.D. .18356 .14575 .18269 .12 N 20 21 20  of Male Pups MEAN 1.9522 1.9402 2.0129 2.0 S.D. .21156 .17893 .17334 .15 N 20 21 20  of Female Pups MEAN 2.0712 2.0379 2.1095 2.1 S.D. .18942 .15056 .18912 .19 N 20 21 19 SPLEEN MEAN 0.4169 0.4114 0.4183 0.3 S.D. .04349 .05104 .04353 .03 N 20 21 20  of Male Pups MEAN 0.4230 0.4125 0.4235 0.3 S.D. .05278 .07058 .05118 .05 N 20 21 20  of Female Pups MEAN 0.4107 0.4103 0.4115 0.3 S.D. .05451 .04627 .05162 .05 N 20 21 19 THYMUS MEAN 0.4572 0.4504 0.4911 0.4 S.D. .04710 .05477 .05135 .03 N 20 21 20  of Male Pups MEAN 0.4443 0.4123 0.4731 0.4 S.D. .06135 .07036 .06382 .04 N 20 21 20  of Female Pups MEAN 0.4700 0.4885 0.5070 0.5 S.D. .05496 .05513 .05892 .06 N 20 21 19 ------------------------------------------------------------------------------------------------------------------No statistically significant differences 
23-JUN-2009 14:14 

Huntingdon Life Sciences 00-4206  TABLE 22  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  SUMMARY OF PUP ORGAN WEIGHT DATA  PUP ORGAN WEIGHT TO BRAIN WEIGHT RATIO ------------------------------------------------------------------------------------------------------------------ DOSE GROUP I II III I DOSE LEVEL (MG/M3) 0 2000 10000 200------------------------------------------------------------------------------------------------------------------SPLEEN MEAN 20.878 20.711 20.622 18. S.D. 2.3979 1.8125 2.9267 1.8 N 20 21 20  of Male Pups MEAN 21.822 21.266 21.199 19. S.D. 3.0676 3.0595 3.1438 2.5 N 20 21 20  of Female Pups MEAN 19.934 20.156 19.671 17. S.D. 2.9284 1.9680 3.1341 2.1 N 20 21 19 THYMUS MEAN 22.953 22.699 24.181 22. S.D. 3.2980 2.6458 3.6712 2.4 N 20 21 20  of Male Pups MEAN 22.987 21.368 23.639 21. S.D. 3.9137 3.9079 3.6607 2.6 N 20 21 20  of Female Pups MEAN 22.918 24.029 24.252 23. S.D. 3.6502 2.6330 3.9064 3.4 N 20 21 19 ------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01 
Huntingdon Life Sciences 00-4206 Page 119 

211-TBA-1G Final Report

Lesion Incidence Summary

with Expanded Severity Levels 

(P0 Generation) 

Preface Table 23

Key to Abbreviations

Gl = Gland

Nad = No abnormal diagnoses 

Ctls = Controls (Group I) 

LN = Lymph Node 

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m3

Group IV - 20000 mg/m3
Page 
Page 
Page 
Page 
Page 
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Inhalation Report Appendix A

TABLE OF CONTENTS

1. INTRODUCTION..............................................................................................126

2. MATERIALS AND METHODS

2.1. Husbandry During Exposure Periods .......................................................126 

2.2. Test Substance Administration and Chamber Operation .........................127 

2.3. Exposure Concentration Determination ...................................................131 

3. TABLES

I. Summary of In-Chamber Observations ...................................................133 

II. Chamber Monitoring Results ...................................................................142 

III. Equipment List .........................................................................................164 

IV. Chamber Distribution Records ................................................................167 

V. Miran Calibration .....................................................................................169 

VI. Testing Room Environmental Monitoring ...............................................172 

4. FIGURES

I. Chamber Generation System and Whole-Body Exposure 

Chamber – Group I ..............................................................................173 

II. Chamber Generation System and Whole-Body Exposure 

Chamber – Groups II, III, IV ...............................................................174 
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Inhalation Report Appendix A

1. INTRODUCTION

This appendix presents the methodology for exposure atmosphere generation 

monitoring and results. 

2. MATERIALS AND METHODS 

2.1. HUSBANDRY DURING EXPOSURE PERIODS

2.1.1. HOUSING

Animals were individually housed in stainless steel, wire mesh 

cages within a 1.5 m3 glass and stainless steel whole-body 

exposure chamber. The placement of the animals in the wholebody exposure chamber was rotated once weekly to ensure uniform 

exposure of the animals. A description of the animal rotation is 

included in the raw data. 

2.1.2. FEED

None was provided during exposure. 

2.1.3. WATER

None was provided during exposure. 

2.1.4. ENVIRONMENTAL CONDITIONS

Chamber temperature and relative humidity every half-hour during 

exposure and maintained, to the maximum extent possible, within 

the ranges presented below. Excursions outside the specified range 

did not affect the integrity of the study. 

Temperature

Desired: 20 to 24°C 

Actual: 18 to 28°C 

Average: 21 to 27°C 
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Inhalation Report Appendix A

Relative Humidity

Desired: 40 to 60% 

Actual: 25 to 82% 

Average: 30 to 68% 

2.2. TEST SUBSTANCE ADMINISTRATION AND CHAMBER 

OPERATIONS 

2.2.1. ROUTE OF ADMINISTRATION

Inhalation via whole-body exposures 

2.2.2. TEST SUBSTANCE ADMINISTRATION

The test substance was administered as a vapor in the breathing air 

of the animals. The test atmosphere was generated by an 

appropriate procedure determined during the pre-study trials. The 

trials were performed to evaluate the optimal set of conditions and 

equipment to generate a stable atmosphere at the target exposure 

levels and maintain uniform conditions throughout the exposure 

chambers.

2.2.3. JUSTIFICATION FOR ROUTE OF ADMINISTRATION 

The inhalation route is one of the potential routes of human 

exposure to this test substance and is the route specified in the 

referenced US EPA 79.61 guidelines and US OPPTS 870.3800 

guidelines. 

2.2.4. TARGET EXPOSURE LEVELS

Group I - 0 mg/m3

Group II - 2,000 mg/m3

Group III - 10,000 mg/m3

Group IV - 20,000 mg/m3
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Inhalation Report Appendix A

2.2.5. JUSTIFICATION FOR EXPOSURE LEVEL SELECTION

Exposure levels were selected by the Sponsor, based on results 

from a 13-week inhalation study conducted at the Testing Facility, 

that utilized this test substance in rats (00-6131). The exposure 

levels were also selected based on the lower flammable limits of 

the test substance.

2.2.6. FREQUENCY OF ADMINISTRATION

Frequency 

P0 males and females received 70 consecutive days (ten weeks) of 

exposure prior to mating for six hours/day. P0 males and females 

continued to be exposed daily throughout a 14-day cohabitation 

period. The mated females continued to be exposed daily from 

Day 0 through 19 of gestation. Beginning on Day 5 of lactation, 

nursing P0 females were exposed daily until weaning of the F1

offspring on Day 28. P0 females with no confirmed day of mating 

but with evidence of pregnancy (weight gain) continued to be 

exposed until the presumed Day 19 of gestation. P0 females with 

no confirmed day of mating and with no evidence of pregnancy 

(weight gain) were exposed for 25 days following completion of 

the mating period and then euthanized. P0 females with a 

confirmed day of mating that did not deliver were euthanized on 

presumed Day 25 of gestation. 

P0 males were exposed daily until euthanasia, after the last F1

litters were delivered.

2.2.7. DURATION 

The test substance was administered for 6 hours/day during all 

segments of the study. 
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Inhalation Report Appendix A

2.2.8. EXPOSURE PROCEDURE AND CHAMBER OPERATIONS

Group I

Houseline nitrogen was delivered from a regulator with a 

backpressure gauge via 1⁄4” tubing to a flowmeter regulated by a 

metering valve. This nitrogen flow (~ 20 Lpm) was then directed 

into the turret of the 1.5 m3 glass and stainless steel exposure 

chamber where it was mixed with room air as it was drawn into the 

chamber. This nitrogen flow simulated the generation nitrogen 

flow for Groups II, III, and IV. 

Groups II, III and IV

Houseline nitrogen was delivered from a regulator with a

backpressure gauge through a stainless steel fitting to create three 

flow systems: the test substance pressurization flow, the purge flow 

and the volatilization flow.

The nitrogen for the test substance pressurization flow was directed 

via 1⁄4” tubing through a metering valve, attached to a backpressure 

gauge, into the vapor inlet valve of the test substance cylinder. The 

metering valve was used to adjust and maintain the pressure within 

the cylinder. From the pressurized cylinder, the test substance 

flowed from the liquid outlet valve through a quick-disconnect 

fitting and through a filter to prevent equipment contamination. 

From the filter, the test substance flowed to a liquid flowmeter via 

1

/8” tubing. The outlet of the flowmeter was regulated by a built-in 

metering valve. From this metering valve, the test substance 

flowed via 1/8” tubing onto the glass helix of a counter current 

volatilization chamber. The glass helix was heated by a nichrome 

wire, which was controlled by an autotransformer and was inserted 

in the center of the glass tube that supported the helix. 

The nitrogen for the purge flow system was directed, via 1⁄4” tubing 

to a flowmeter regulated by a metering valve. The purge nitrogen 

was delivered via 1/8” tubing to the bottom of the tube containing 

the nichrome wire. This nitrogen flow continuously purged the 
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211-TBA-1G Final Report

Inhalation Report Appendix A

area surrounding the nichrome wire within the tube, thereby 

protecting the wire from oxidation. 

The nitrogen for the volatilization system was directed via 1⁄4” 

tubing to a flowmeter regulated by a metering valve. From the 

flowmeter, the volatilization nitrogen (~ 18 Lpm for Group II and 

~ 20 Lpm for Groups III and IV ) flowed via 1⁄4” tubing to a ball 

and socket joint at the bottom of the volatilization chamber. This 

nitrogen flowed up through the volatilization chamber passing over 

the coil and volatilizing the test substance. The pressure within the 

counter-current volatilization chamber was maintained slightly 

negative to the room and was monitored with a pressure gauge. 

This test substance laden nitrogen exited the top of the 

volatilization chamber through a glass elbow, which directed the 

flow, via 1⁄2” tubing, to the turret of 1.5 m3 glass and stainless steel 

exposure chamber. As the test substance laden nitrogen was drawn 

into the chamber, it was mixed with room air.

The whole-body exposure chambers each had a volume of 

approximately 1500 Liters (1.5 m3). Each chamber was operated at 

a minimum flow rate of 300 Liters per minute. The final airflow 

was set to provide at least one air change (calculated by dividing 

the chamber volume by the airflow rate) in 5.0 minutes (12 air 

changes/hour) and a T99 equilibrium time (calculated by 

multiplying the air change by the exponential factor 4.6) of at most 

23 minutes: 

Group Airflow Rate Air Change T99

(Lpm) (min) (min)

I 317 4.7 22

II 312 4.8 22

III 320 4.7 22

IV 303 5.0 23

This chamber size and airflow rate was considered adequate to 

maintain the oxygen level at least 19% and the animal-loading 
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Inhalation Report Appendix A

factor below 5%. Chamber temperature, humidity, airflow rate and 

static pressure were monitored continuously and recorded every 

30 minutes during exposure. At the end of the 6-hour exposure, all 

animals remained in the chamber for a minimum of 30 minutes. 

During this time the chamber was operated at approximately the 

same flow rate using clean air only. The chambers were exhausted 

through the in housed filtering system, which consisted of a coarse 

filter, a HEPA filter and activated charcoal.

See Chamber Figures I and II and Table III for equipment details.

2.3. EXPOSURE CONCENTRATION DETERMINATION

2.3.1. NOMINAL COCENTRATION

A nominal exposure concentration was calculated. The flow of air 

through the chamber was monitored using appropriate calibrated 

equipment. The test substance consumed (weight difference of the 

test substance cylinder) during the exposure (mg) was divided by 

the total volume of air (m3) passing through the chamber 

(volumetric flow rate times total exposure time) to give the 

nominal concentration (mg/m3).

2.3.2. CHAMBER SAMPLING

During each exposure, measurements of airborne concentrations 

were performed in the animals’ breathing zone at least 4 times 

using an appropriate sampling procedure (~ 20 Lpm) and Infra-Red 

Spectrophotometric (IR) analytical method. Also, one charcoal 

tube sample per chamber per week was collected (at ~ 20 Lpm) and

was analyzed by gas chromatography (GC) to characterize at least 

10 major components (comprising at least 80% by weight of the 

test substance) to show test substance stability and comparison 

between the neat liquid test substance and the vaporized test 

atmospheres.

See Table III for equipment details. See Appendix B for Analytical 

Report. 
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Inhalation Report Appendix A

2.3.3. PARTICLE SIZE DISTRIBUTION 

During each week of exposure, particle size determinations were 

performed using a TSI Aerodynamic Particle Sizer to characterize 

the aerodynamic particle size distribution of any aerosol present. 

Samples were drawn for 20 seconds at 5 Lpm. The mass median 

aerodynamic diameter, geometric standard deviation and total 

concentration were calculated based on the amount of particle 

collected.

See Table III for equipment details.

2.3.4. CHAMBER AND EXPOSURE ROOM ENVIRONMENT

Chamber oxygen levels (maintained at least 19%) were measured 

pretest and at the beginning, middle, and end of the study. 

Air samples were taken in the vapor generation area pretest and at 

the beginning, middle and end of the study. Light (maintained 

approximately 30 foot-candles at 1.0 meters above the floor) and 

noise levels (maintained below 85 decibels) in the exposure room 

were measured pretest and at the beginning, middle and end of the 

study. 
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All - -

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All -

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All - -

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations 

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 129 130

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All
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Table II

Chamber Monitoring Results 

Preface

Key to Abbreviations 

MMAD = Mass Median Aerodynamic Diameter

GSD = Geometric Standard Deviation

TMC = Total Mass Concentration





















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Table III

Equipment List 

Exposure Chamber

1500 Liter glass and stainless steel chamber (Unifab Cages).

Chamber Air-flow Gauges

Dwyer® Magnehelic® gauge (Dwyer Instruments Inc.), calibrated prestudy with a Side 

Trak III, equipped with a Digital Meter, Model 831-N2 (Sierra Instruments, Inc.). 

Chamber Static Pressure

Dwyer® Magnehelic® gauge (Dwyer® Instruments Inc.); calibrated prestudy with a 

Dwyer® Mark II Manometer, Model 25 (Dwyer® Instruments Inc.) 

Compound Generator

Counter-Current Volatilization Unit, coiled glass rod insert with nichrome wire 

 (Crown Glass Co., Inc.). 

Flowmeter with built-in valve, size 0-65 mm, tube #’s 6G02R3, 6G03R3, 6G04R3 

 (Key Instruments). 

Variable Autotransformers: Type 3PN 1010 (Staco Energy Products Company) 

and Type PL 312, Minitrol (Glas-col® Apparatus Company) 

T° Sentry Digital Alarm Module, Model 110 (Hampshire Controls Corp.). 

Balston® Microfibre Disposable Filter Units, No. L9933-05 (Grade DQ).

Quick-disconnect fitting with toggle valve (Rego).

Compound Reservoir

5-gallon cylinder (Manchester Tank).

Balance 

Mettler PM30000K (Mettler Instrument Corporation). 

Pelouze, Model No. 4010. 

Flowmeters

Flowmeter, size 0-4, 0-5, 0-20, 0-30, 0-40 Lpm (Dwyer® Instruments Inc.). 

Top Trak Mass Flow Meter size 0-1 Lpm, Model 821-1 (Sierra Instruments), 

calibrated prestudy with a GilibratorBubble Generator, P/N D800286, S/N 569-S, flow 

cell assembly P/N D80026B, BD #1860). 
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Inhalation Report Appendix A

Table III

Equipment List 

Pressure/Vacuum Gauges

Ashcroft backpressure gauge, P/N 733-47 

Matheson® backpressure gauge, P/N 63-3161. 

Norgreen backpressure gauge, P/N 9892K23. 

Dwyer® Magnehelic® gauge (Dwyer® Instruments Inc.). 

Gast® vacuum gauge, Model 169-01 

Union Carbide backpressure gauge (P/N SG 8383) 

Regulator

Norgreen (P/N 9892K23) 

Valves 

Metering Valve, Model SS-4L Series (Nupro® Co.). 

Tubing

Teflon®, size 1/8”, 1/4”, 1/2”. 

Plastic Size 1/4”, 1/2” (Norton, Baxter). 

Stainless steel 1/2”. 

T-Tube, stainless steel.

Glass elbow tube (Crown Glass Co.). 

Stainless steel cross (Swage).

Filter

Balston® Microfibre Disposable Filter Unit, No. L9933-05 (Grade DQ).

Air Analyzer

MIRAN® 1A-CVF Ambient Air Analyzer (Wilks) with a Cole Parmer strip recorder 

Model 201 and a Micronta® LCD Benchtop Digital Multimeter No. 22-195. 

Oxygen/Gas Analyzer, Model 12145 (Gastech). 

Digital Sound Level Meter, Model 840029 (Speer Scientific).

Photometer/Light Meter (Quantum Instruments).

Particle Sizer/Analyzer
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Inhalation Report Appendix A

Table III

Equipment List 

TSI Aerodynamic Particle Sizer, Model 331001 and a DELL computer, Model 486P/25, 

equipped with an Epson LQ-570+ Dot matrix printer, Model P630B. 

Absorbent Tube Sampling

Charcoal Tubes, ORBO-32, Lot #’s 15350 and 13843 (Supelco). 

Vacuum Pumps

Thomas Industries Inc., Model 707CM50, 107CA183. 

Timer

Gralab Universal Timer, Model 171. 

Environmental Monitoring

VWR Big Digit Temperature and Humidity Gauge, tested prestudy with a VWR NIST 

Traceable Digital Hygrometer/Thermometer.
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Inhalation Report Appendix A

Table IV

Chamber Distribution Records 

Group

(target)

Date Port IR Conc

(mg/m3)

Ratio to H-3

II (2,000 mg/m3) 12-August-02 H-3 1950 1.00

H-1 1420 0.73

H-2 1970 1.01

H-9 1700 0.87

H-3 1920 1.00

H-7 1360 0.71

H-8 1960 1.02 

H-11 1410 0.73

H-3 2080 1.00

H-12 2160 1.03

H-13 1660 0.80

H-14 2210 1.06

H-3 2200 1.00

H-1 1660 0.75

H-7 1690 0.77 

H-11 1680 0.76

II (2,000 mg/m3) 14-August-02 H-3 2260 1.00

H-1 1720 0.76

H-7 1580 0.70

H-11 1660 0.73

II (2,000 mg/m3) 15-August-02 H-3 1950 1.00

H-1 1920 0.98 

H-2 1900 0.97

H-9 1900 0.97

H-7 1910 0.98

H-3 1950 1.00

H-8 1920 0.98

H-11 1930 0.99

H-12 1910 0.98

H-13 1940 0.99 

H-14 1910 0.98
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Inhalation Report Appendix A

Table IV

Chamber Distribution Records 

Group

(target)

Date Port IR Conc

(mg/m3)

Ratio to H-3

III (10,000 mg/m3) 13-August-02 H-3 10100 1.00

H-1 10000 0.99

H-2 9940 0.98

H-9 9780 0.97

H-3 9720 1.00

H-7 9750 1.00

H-8 9590 0.99 

H-11 9560 0.98

H-3 9560 1.00

H-12 9490 0.99

H-13 9300 0.97

H-14 9270 0.97

IV (20,000 mg/m3) 14-August-02 H-3 19500 1.00

H-1 19300 0.99 

H-2 21000 1.08

H-9 18800 0.96

H-3 19800 1.00

H-7 17900 0.90

H-8 20400 1.03

H-11 20900 1.06

H-3 20500 1.00

H-12 21600 1.05

H-13 21300 1.04 

H-14 22100 1.08
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Inhalation Report Appendix A

Table V

Miran Calibration

Methodology for Gasoline TBA Vapor Condensate 

Settings: The instrument settings for the Miran 04 Unit are summarized below: 

wavelength, microns 10.4 

pathlength, dial setting 5.68 

slit width, mm 1 

range, absorbance 1A 

response, seconds 1 

gain High 

chart speed, cm/min 1 

chart volts 1 

Calibrations: The Miran® was turned on and allowed to warm up for approximately 

10 minutes. The cell was flushed with room air for approximately one minute. The loop 

was closed, the unit was zeroed and the calibration series was performed as shown below. 

The resultant data were plotted to obtain a calibration curve. Each observer used a 

separate syringe for calibration.

Injection Calculated Absorbance . 

Volume Concentration1 Operator 1 Operator 2 Average

(μL) (mg/m3) (volts) (volts) (volts) 

 9 1053 0.0485 0.0344 0.0415 

 17 1989 0.0717 0.0652 0.0685 

 86 10064 0.331 0.325 0.328 

 170 19894 0.632 0.631 0.632 

 210 24575 0.748 0.770 0.759 

1

Calculated Conc. (mg/m3) = Injection volume (μL) x Density ( 0.66 mg/μL) x 1000 L/m3

 5.64 L (Volume of Miran cell) 
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Inhalation Report Appendix A

Table V

Miran Calibration

Calibration Checks: A three-point calibration check of the Miran® was performed for 

each exposure prior to sampling the chambers. The parameters are shown below: 

 Expected Acceptable

Injection Calculated Absorbance Absorbance 

 Volume Concentration Reading Range

(μL) (mg/m3) (volts) (volts) 

 

 17 1989 0.0685 0.0582 – 0.0788 

 86 10064 0.328 0.279 – 0.377 

 170 19894 0.632 0.537 – 0.727 

The absorbance was recorded after each injection. The absorbance was considered 

satisfactory if it was within 15% of the original calibration series. If any of the 

absorbance values fell outside the 15% range, the injection was rechecked as follows. 

The volume for the value that was out of range was reinjected twice. The closer pair of 

the three injections were averaged and the results were compared to the original curve. If 

the average of the pair was within the 15% range, the original was accepted. If the value 

of the average was outside the 15% range, the Study Director decided if a new graph was 

to be prepared. 
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Inhalation Report Appendix A

Table V

Miran Calibration

Calibration Curve for Gasoline TBA Vapor Condensate 

(Groups I – IV)

y = 22403x4

 - 33856x3 + 19834x2 + 26953x

R2

 = 0.9994

0

5000

10000

15000

20000

25000

30000

0.0000 0.2000 0.4000 0.6000 0.8000 1.0000

Absorbance (Volts)

Concentration (mg/m3)
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Inhalation Report Appendix A

Table VI

Testing Room Environmental Monitoring 

Interval Location

Test

Substance

(mg/m3)

Light 

(Ft Candles)

Noise

(dB)

Oxygen 

(%)

Particle

Sizing

(mg/m3)

a

Pretest Room 809 0 33.4 60.5 -

Group I Chamber - - - 20 3.26 x 10-3

Group II Chamber - - - 20 1.99 x 10-3

Group III Chamber - - - 20 3.51 x 10-3

Group IV Chamber - - - 20 2.52 x 10-3

Week 0 Room 809 0 34.5 62.0 -

Group I Chamber - - - 20

Group II Chamber - - - 20

Group III Chamber - - - 20 

Group IV Chamber - - - 20 

Week 9 Room 809 0 37.2 66.8 -

Group I Chamber - - - 20

Group II Chamber - - - 20

Group III Chamber - - - 20

Group IV Chamber - - - 20

Week 17 Room 809 0 34.8 65.3 - 

Group I Chamber - - - 19

Group II Chamber - - - 20

Group III Chamber - - - 20

Group IV Chamber - - - 20

a

Pretest results presented below. For Weeks 0, 9 and 17 results, see Chamber Monitoring 

Records (Table II).
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Group I Inhalation Report Appendix A

Figure I

Chamber Generation System 

and Whole-Body Exposure Chamber

Notes:

1. Sampling Ports H-11 (left-bottom), H-12 (left-top), H-13 (right-bottom) and H-14 

(right-top) used for pretest distribution sampling, were located on the back wall of the 

chambers.

2. Animals were individually housed on five levels within the exposure chamber.
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Groups II, III and 

IV Inhalation Report Appendix A

Figure II

Chamber Generation System 

and Whole-Body Exposure Chamber 

Notes:

1. Sampling Ports H-11 (left-bottom), H-12 (left-top), H-13 (right-bottom) and H-14 

(right-top) used for pretest distribution sampling, were located on the back wall of the 

chambers.

2. Animals were individually housed on five levels within the exposure chamber. 































23-JUN-2009 17:31

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 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 TERMINAL SACRIFICE 11-DEC-02 113 

 1027 TERMINAL SACRIFICE 11-DEC-02 113 

 1028 TERMINAL SACRIFICE 11-DEC-02 113 

 1029 TERMINAL SACRIFICE 11-DEC-02 113 

 1030 TERMINAL SACRIFICE 11-DEC-02 113 

 1031 TERMINAL SACRIFICE 11-DEC-02 113 

 1032 TERMINAL SACRIFICE 11-DEC-02 113 

 1033 TERMINAL SACRIFICE 11-DEC-02 113 

 1034 TERMINAL SACRIFICE 11-DEC-02 113 

 1035 TERMINAL SACRIFICE 11-DEC-02 113 

 1036 TERMINAL SACRIFICE 11-DEC-02 113 

 1037 TERMINAL SACRIFICE 11-DEC-02 113 

 1038 TERMINAL SACRIFICE 11-DEC-02 113 

 1039 TERMINAL SACRIFICE 12-DEC-02 114 

 1040 TERMINAL SACRIFICE 12-DEC-02 114 

 1041 TERMINAL SACRIFICE 12-DEC-02 114 

 1042 TERMINAL SACRIFICE 12-DEC-02 114 

 1043 TERMINAL SACRIFICE 12-DEC-02 114 

 1044 TERMINAL SACRIFICE 12-DEC-02 114 

 1045 TERMINAL SACRIFICE 12-DEC-02 114 

 1046 TERMINAL SACRIFICE 12-DEC-02 114 

 1047 TERMINAL SACRIFICE 12-DEC-02 114 

 1048 TERMINAL SACRIFICE 12-DEC-02 114 

 1049 TERMINAL SACRIFICE 12-DEC-02 114 

 1050 TERMINAL SACRIFICE 12-DEC-02 114 

 1051 TERMINAL SACRIFICE 12-DEC-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 207 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 TERMINAL SACRIFICE 11-DEC-02 113 

 2027 TERMINAL SACRIFICE 11-DEC-02 113 

 2028 TERMINAL SACRIFICE 11-DEC-02 113 

 2029 TERMINAL SACRIFICE 11-DEC-02 113 

 2030 TERMINAL SACRIFICE 11-DEC-02 113 

 2031 TERMINAL SACRIFICE 11-DEC-02 113 

 2032 TERMINAL SACRIFICE 11-DEC-02 113 

 2033 TERMINAL SACRIFICE 11-DEC-02 113 

 2034 TERMINAL SACRIFICE 11-DEC-02 113 

 2035 TERMINAL SACRIFICE 11-DEC-02 113 

 2036 TERMINAL SACRIFICE 11-DEC-02 113 

 2037 TERMINAL SACRIFICE 11-DEC-02 113 

 2038 TERMINAL SACRIFICE 11-DEC-02 113 

 2039 TERMINAL SACRIFICE 12-DEC-02 114 

 2040 TERMINAL SACRIFICE 12-DEC-02 114 

 2041 TERMINAL SACRIFICE 12-DEC-02 114 

 2042 TERMINAL SACRIFICE 12-DEC-02 114 

 2043 TERMINAL SACRIFICE 12-DEC-02 114 

 2044 TERMINAL SACRIFICE 12-DEC-02 114 

 2045 TERMINAL SACRIFICE 12-DEC-02 114 

 2046 TERMINAL SACRIFICE 12-DEC-02 114 

 2047 TERMINAL SACRIFICE 12-DEC-02 114 

 2048 TERMINAL SACRIFICE 12-DEC-02 114 

 2049 TERMINAL SACRIFICE 12-DEC-02 114 

 2050 TERMINAL SACRIFICE 12-DEC-02 114 

 2051 TERMINAL SACRIFICE 12-DEC-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 208 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 TERMINAL SACRIFICE 11-DEC-02 113 

 3027 TERMINAL SACRIFICE 11-DEC-02 113 

 3028 TERMINAL SACRIFICE 11-DEC-02 113 

 3029 TERMINAL SACRIFICE 11-DEC-02 113 

 3030 TERMINAL SACRIFICE 11-DEC-02 113 

 3031 TERMINAL SACRIFICE 11-DEC-02 113 

 3032 TERMINAL SACRIFICE 11-DEC-02 113 

 3033 TERMINAL SACRIFICE 11-DEC-02 113 

 3034 TERMINAL SACRIFICE 11-DEC-02 113 

 3035 TERMINAL SACRIFICE 11-DEC-02 113 

 3036 TERMINAL SACRIFICE 11-DEC-02 113 

 3037 TERMINAL SACRIFICE 11-DEC-02 113 

 3038 TERMINAL SACRIFICE 11-DEC-02 113 

 3039 TERMINAL SACRIFICE 12-DEC-02 114 

 3040 TERMINAL SACRIFICE 12-DEC-02 114 

 3041 TERMINAL SACRIFICE 12-DEC-02 114 

 3042 TERMINAL SACRIFICE 12-DEC-02 114 

 3043 TERMINAL SACRIFICE 12-DEC-02 114 

 3044 TERMINAL SACRIFICE 12-DEC-02 114 

 3045 TERMINAL SACRIFICE 12-DEC-02 114 

 3046 TERMINAL SACRIFICE 12-DEC-02 114 

 3047 TERMINAL SACRIFICE 12-DEC-02 114 

 3048 TERMINAL SACRIFICE 12-DEC-02 114 

 3049 TERMINAL SACRIFICE 12-DEC-02 114 

 3050 TERMINAL SACRIFICE 12-DEC-02 114 

 3051 TERMINAL SACRIFICE 12-DEC-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 209 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 TERMINAL SACRIFICE 11-DEC-02 113 

 4027 TERMINAL SACRIFICE 11-DEC-02 113 

 4028 TERMINAL SACRIFICE 11-DEC-02 113 

 4029 TERMINAL SACRIFICE 11-DEC-02 113 

 4030 TERMINAL SACRIFICE 11-DEC-02 113 

 4031 TERMINAL SACRIFICE 11-DEC-02 113 

 4032 TERMINAL SACRIFICE 11-DEC-02 113 

 4033 TERMINAL SACRIFICE 11-DEC-02 113 

 4034 TERMINAL SACRIFICE 11-DEC-02 113 

 4035 TERMINAL SACRIFICE 11-DEC-02 113 

 4052 TERMINAL SACRIFICE 11-DEC-02 113 

 4037 TERMINAL SACRIFICE 11-DEC-02 113 

 4038 TERMINAL SACRIFICE 11-DEC-02 113 

 4039 TERMINAL SACRIFICE 12-DEC-02 114 

 4040 TERMINAL SACRIFICE 12-DEC-02 114 

 4041 TERMINAL SACRIFICE 12-DEC-02 114 

 4042 TERMINAL SACRIFICE 12-DEC-02 114 

 4043 TERMINAL SACRIFICE 12-DEC-02 114 

 4044 TERMINAL SACRIFICE 12-DEC-02 114 

 4045 TERMINAL SACRIFICE 12-DEC-02 114 

 4046 TERMINAL SACRIFICE 12-DEC-02 114 

 4047 TERMINAL SACRIFICE 12-DEC-02 114 

 4048 TERMINAL SACRIFICE 12-DEC-02 114 

 4049 TERMINAL SACRIFICE 12-DEC-02 114 

 4050 TERMINAL SACRIFICE 12-DEC-02 114 

 4051 TERMINAL SACRIFICE 12-DEC-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 210 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 TERMINAL SACRIFICE 18-DEC-02 120 P 

 1527 DPP FOUND DEAD 24-NOV-02 96 P 

 1528 TERMINAL SACRIFICE 21-DEC-02 123 P 

 1529 PNMD TERMINAL SACRIFICE 25-DEC-02 127 P 

 1530 TERMINAL SACRIFICE 21-DEC-02 123 P 

 1531 TERMINAL SACRIFICE 18-DEC-02 120 P 

 1532 TERMINAL SACRIFICE 26-NOV-02 98 NP

 1533 TERMINAL SACRIFICE 18-DEC-02 120 P 

 1534 TERMINAL SACRIFICE 18-DEC-02 120 P 

 1535 TERMINAL SACRIFICE 24-NOV-02 96 NP

 1536 TERMINAL SACRIFICE 23-DEC-02 125 P 

 1537 TERMINAL SACRIFICE 20-DEC-02 122 P 

 1538 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1539 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1540 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1541 TERMINAL SACRIFICE 25-NOV-02 97 NP

 1542 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1543 TERMINAL SACRIFICE 24-DEC-02 126 P 

 1544 TERMINAL SACRIFICE 21-DEC-02 123 P 

 1545 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1546 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1547 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1548 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 1549 TERMINAL SACRIFICE 19-DEC-02 121 P 

 1550 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 1551 TERMINAL SACRIFICE 20-DEC-02 122 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 211 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP II 2000 MG/M3 

----------------------------------------------------------------------------------------------------------------------------------- 

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 TERMINAL SACRIFICE 19-DEC-02 121 P 

 2527 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 2528 TERMINAL SACRIFICE 20-DEC-02 122 P 

 2529 TERMINAL SACRIFICE 21-DEC-02 123 P 

 2530 TERMINAL SACRIFICE 26-DEC-02 128 P 

 2531 TERMINAL SACRIFICE 20-DEC-02 122 P 

 2532 TERMINAL SACRIFICE 25-NOV-02 97 NP

 2533 TERMINAL SACRIFICE 19-DEC-02 121 P 

 2534 TERMINAL SACRIFICE 21-DEC-02 123 P 

 2535 TERMINAL SACRIFICE 18-DEC-02 120 P 

 2536 TERMINAL SACRIFICE 22-DEC-02 124 P 

 2537 TERMINAL SACRIFICE 18-DEC-02 120 P 

 2538 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 2539 TERMINAL SACRIFICE 19-DEC-02 121 P 

 2540 TERMINAL SACRIFICE 21-DEC-02 123 P 

 2541 TERMINAL SACRIFICE 19-DEC-02 121 P 

 2542 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 2543 TERMINAL SACRIFICE 19-DEC-02 121 P 

 2544 TERMINAL SACRIFICE 21-DEC-02 123 P 

 2545 TERMINAL SACRIFICE 21-DEC-02 123 P 

 2546 TERMINAL SACRIFICE 19-DEC-02 121 P 

 2547 TERMINAL SACRIFICE 22-DEC-02 124 P 

 2548 TERMINAL SACRIFICE 20-DEC-02 122 P 

 2549 TLL TERMINAL SACRIFICE 17-DEC-02 119 P 

 2550 TERMINAL SACRIFICE 18-DEC-02 120 P 

 2551 TERMINAL SACRIFICE 20-DEC-02 122 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS 
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 212 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 TERMINAL SACRIFICE 24-NOV-02 96 NP

 3527 TERMINAL SACRIFICE 18-DEC-02 120 P 

 3528 TERMINAL SACRIFICE 22-DEC-02 124 P 

 3529 TERMINAL SACRIFICE 17-DEC-02 119 P 

 3530 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3531 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3532 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3533 TERMINAL SACRIFICE 20-DEC-02 122 P 

 3534 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3535 TERMINAL SACRIFICE 25-DEC-02 127 P 

 3536 TLL TERMINAL SACRIFICE 17-DEC-02 119 P 

 3537 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3538 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 3539 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 3540 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3541 TERMINAL SACRIFICE 18-DEC-02 120 P 

 3542 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3543 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3544 TERMINAL SACRIFICE 21-DEC-02 123 P 

 3545 TERMINAL SACRIFICE 19-DEC-02 121 P 

 3546 TERMINAL SACRIFICE 22-DEC-02 124 P 

 3547 TERMINAL SACRIFICE 20-DEC-02 122 P 

 3548 TLL TERMINAL SACRIFICE 17-DEC-02 119 P 

 3549 TERMINAL SACRIFICE 22-DEC-02 124 P 

 3550 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 3551 TERMINAL SACRIFICE 19-DEC-02 121 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS 
23-JUN-2009 17:31

Huntingdon Life Sciences 00-4206 PAGE 213 

 

 APPENDIX C 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 TERMINAL SACRIFICE 25-DEC-02 127 P 

 4527 TERMINAL SACRIFICE 21-DEC-02 123 P 

 4528 TERMINAL SACRIFICE 22-DEC-02 124 P 

 4529 TERMINAL SACRIFICE 22-DEC-02 124 P 

 4530 TERMINAL SACRIFICE 27-NOV-02 99 NP

 4531 TERMINAL SACRIFICE 20-DEC-02 122 P 

 4532 TERMINAL SACRIFICE 23-DEC-02 125 P 

 4533 TERMINAL SACRIFICE 18-DEC-02 120 P 

 4534 TERMINAL SACRIFICE 22-DEC-02 124 P 

 4535 TERMINAL SACRIFICE 18-DEC-02 120 P 

 4536 TERMINAL SACRIFICE 19-DEC-02 121 P 

 4537 TERMINAL SACRIFICE 24-DEC-02 126 P 

 4538 TERMINAL SACRIFICE 18-DEC-02 120 P 

 4539 TERMINAL SACRIFICE 24-NOV-02 96 NP

 4540 TERMINAL SACRIFICE 18-DEC-02 120 P 

 4541 NNMD TERMINAL SACRIFICE 7-DEC-02 109 NP

 4542 TERMINAL SACRIFICE 20-DEC-02 122 P 

 4543 TERMINAL SACRIFICE 19-DEC-02 121 P 

 4544 TERMINAL SACRIFICE 18-DEC-02 120 P 

 4545 TERMINAL SACRIFICE 26-DEC-02 128 P 

 4546 TERMINAL SACRIFICE 20-DEC-02 122 P 

 4547 PNMD TERMINAL SACRIFICE 21-DEC-02 123 P 

 4548 TERMINAL SACRIFICE 20-DEC-02 122 P 

 4549 DIED FOUND DEAD 28-SEP-02 39 - 

 4550 TERMINAL SACRIFICE 29-DEC-02 131 P 

 4551 TERMINAL SACRIFICE 19-DEC-02 121 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

DIED=FOUND DEAD PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 
Huntingdon Life Sciences 00-4206 Page 214 

211-TBA-1G Final Report 

Individual Clinical Observations 

(P0 Generation) 

Preface Appendix D 

For summarization purposes, descriptive com ments [i.e., location of scab(s) and sore(s), 

etc.] are not presented in this appendix. Thes e data are contained in the study raw data if 

needed. 

Corresponding exposure levels for each group were as follows: 

Group I - 0 mg/m3

Group II - 2,000 mg/m3

Group III - 10,000 mg/m3

Group IV - 20,000 mg/m3 

Note: 

If an anim al died am id two scheduled obser vation days, the death is presented at the 

scheduled observation day prior to the death (for example, if an anim al died on Study 

Day 39, its’ death is presented as Study Day 35). Term inal Sacrifices for m ales are 

presented as Study Day 112 rather than St udy Day 119, and term inal sacrifices for 

unmated females are presented as Study Day 105 rather than Study Day 112, due to 

computer limitations. 
24-JUN-2009 11:35 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 1026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1027 WITHIN NORMAL LIMITS P P P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P P P P P P P P P P P  1028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  INCISORS BROKEN/MISSING P P P P  1030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:35 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 1035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1040 WITHIN NORMAL LIMITS P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 3  ALOPECIA - GENERAL 3  1041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1042 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:35 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 1044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1046 WITHIN NORMAL LIMITS P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 3 3 3 2 2 2 2  1047 WITHIN NORMAL LIMITS P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 3 3 3 3 3  CHROMODACRYORRHEA - BILATERAL P  INCISORS MALOCCLUDED P  1048 WITHIN NORMAL LIMITS P P P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P P P P P P P P P P P  INCISORS BROKEN/MISSING P P P P P P P P  ORAL SORE P  1049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 2026 WITHIN NORMAL LIMITS P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2  ULCERATION - CERVICAL 2  2027 WITHIN NORMAL LIMITS P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 3 3 3 3  2028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ULCERATION - CERVICAL P P P  ALOPECIA - GENERAL 2  2030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2  2031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  INCISORS BROKEN/MISSING P P P  2033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 2034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2038 WITHIN NORMAL LIMITS P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 3 3  2039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - GENERAL 2  2040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2041 WITHIN NORMAL LIMITS P P P P P P P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P P P P P P P  INCISORS BROKEN/MISSING P P P P  2042 WITHIN NORMAL LIMITS P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P P P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 2043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P  INCISORS BROKEN/MISSING P  2044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2048 WITHIN NORMAL LIMITS P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P P P P P P P P P P P P P  INCISORS BROKEN/MISSING P P P P  2049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2050 WITHIN NORMAL LIMITS P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2  INCISORS MALOCCLUDED P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 2051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 3026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3  BLACK/BROWN STAINS ON SNOUT 1  3030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 3035 WITHIN NORMAL LIMITS P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 2 2  3036 WITHIN NORMAL LIMITS P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 2 2 2 2 2 2  3037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3038 WITHIN NORMAL LIMITS P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 2 2 2 2 2  3039 WITHIN NORMAL LIMITS P P P P P P P P  TERMINAL SACRIFICE P  CHROMODACRYORRHEA - BILATERAL P  CHROMODACRYORRHEA - UNILATERAL P  INCISORS MALOCCLUDED P P  INCISORS BROKEN/MISSING P P P P P P P P  3040 WITHIN NORMAL LIMITS P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 2 2 2 2  3041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 3042 WITHIN NORMAL LIMITS P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 2 2 2  3043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3044 WITHIN NORMAL LIMITS P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 3 3 3 3 3 3  ALOPECIA - GENERAL 3 3 3  3045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3046 WITHIN NORMAL LIMITS P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3 3 3 3 2 2 2 2 2 2  INCISORS BROKEN/MISSING P P  3047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 3050 WITHIN NORMAL LIMITS P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 3 3 3 3 2  3051 WITHIN NORMAL LIMITS P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 2 2 2 2 2 2 2 2 2 ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 4026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4027 WITHIN NORMAL LIMITS P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2  4028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4029 WITHIN NORMAL LIMITS P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 2  INCISORS MALOCCLUDED P  INCISORS BROKEN/MISSING P P  4030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2  4033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 4034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4052 WITHIN NORMAL LIMITS P P P P P P P P P  TERMINAL SACRIFICE P  INCISORS MALOCCLUDED P P P P P P P P P  4037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3  4039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4040 WITHIN NORMAL LIMITS P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 3 3 3 2  4041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4042 WITHIN NORMAL LIMITS P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 2 2 2 2 2 ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:34 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 ------------------------------------------------------------------------------------------------------------------ 4043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2  4044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4047 WITHIN NORMAL LIMITS P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 2 2 2  4048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 1526 WITHIN NORMAL LIMITS P P P P P P P P P P P  1527 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1528 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1529 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  1530 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1531 WITHIN NORMAL LIMITS P P P P P P P P P P P  1532 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1533 WITHIN NORMAL LIMITS P P P P P P P P P P P  1534 WITHIN NORMAL LIMITS P P P P P P P P P P P  1535 WITHIN NORMAL LIMITS P P P P P P P P P P P  1536 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1537 WITHIN NORMAL LIMITS P P P P P P P P P  INCISORS MALOCCLUDED P P P  1538 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1539 WITHIN NORMAL LIMITS P P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 1540 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1541 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1542 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1543 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1544 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1545 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1546 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1547 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1548 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1549 WITHIN NORMAL LIMITS P P P P P P P P P P P P  1550 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  1551 WITHIN NORMAL LIMITS P P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 2526 WITHIN NORMAL LIMITS P P P P P P  INCISORS MALOCCLUDED P P P P P P  2527 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2528 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2529 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2530 WITHIN NORMAL LIMITS P P P P P P P P P P P P P  2531 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2532 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2533 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2534 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2535 WITHIN NORMAL LIMITS P P P P P P P P P P P  2536 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2537 WITHIN NORMAL LIMITS P P P P P P P P P P P  2538 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 2539 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2540 WITHIN NORMAL LIMITS P P P P P P P  CHROMODACRYORRHEA - UNILATERAL P P  INCISORS MALOCCLUDED P P P P P  2541 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2542 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  2543 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2544 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2545 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2546 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2547 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2548 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2549 WITHIN NORMAL LIMITS P P P P P P P P P P P P  2550 WITHIN NORMAL LIMITS P P P P P P P P P P P  2551 WITHIN NORMAL LIMITS P P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 3526 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3527 WITHIN NORMAL LIMITS P P P P P P P P P P P  3528 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3529 WITHIN NORMAL LIMITS P P P P P P P P P P P  3530 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3531 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3532 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3533 WITHIN NORMAL LIMITS P P P P P P  INCISORS MALOCCLUDED P P P P P P  3534 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3535 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3536 WITHIN NORMAL LIMITS P P P P P P P P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2  3537 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3538 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 3539 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  3540 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3541 WITHIN NORMAL LIMITS P P P P P P P P P P P  3542 WITHIN NORMAL LIMITS P P P P P P P P P P P  CHROMODACRYORRHEA - UNILATERAL P  3543 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3544 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3545 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3546 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3547 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3548 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3549 WITHIN NORMAL LIMITS P P P P P P P P P P P P  3550 WITHIN NORMAL LIMITS P P P P P P P P P  TERMINAL SACRIFICE P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2  ALOPECIA - GENERAL 2 2 2 ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 3551 WITHIN NORMAL LIMITS P P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 4526 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4527 WITHIN NORMAL LIMITS P P P P P P P P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2  4528 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4529 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4530 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4531 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4532 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4533 WITHIN NORMAL LIMITS P P P P P P P P P P P  4534 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4535 WITHIN NORMAL LIMITS P P P P P P P P P P  CHROMODACRYORRHEA - UNILATERAL P  4536 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4537 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4538 WITHIN NORMAL LIMITS P P P P P P  INCISORS MALOCCLUDED P P P P P P  INCISORS BROKEN/MISSING P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 4539 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4540 WITHIN NORMAL LIMITS P P P P P P P P P P P  4541 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P  TERMINAL SACRIFICE P  4542 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4543 WITHIN NORMAL LIMITS P P P P P P P P  ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2  INCISORS MALOCCLUDED P P  INCISORS BROKEN/MISSING P P  4544 WITHIN NORMAL LIMITS P P P P P P P P P P P  4545 WITHIN NORMAL LIMITS P P P P P P P P P P P  YELLOW ANO-GENITAL STAINING 1  4546 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4547 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P  4548 WITHIN NORMAL LIMITS P P P P P P P P P P P P  4549 WITHIN NORMAL LIMITS P P P P P P P  FOUND DEAD P  4550 WITHIN NORMAL LIMITS P P P P P P P P P P P P P ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
24-JUN-2009 11:36 

Huntingdon Life Sciences 00-4206  APPENDIX D  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL WEEKLY CLINICAL OBSERVATIONS FEMALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ 1  DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0  ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 ------------------------------------------------------------------------------------------------------------------ 4551 WITHIN NORMAL LIMITS P P P P P P P P P P  YELLOW ANO-GENITAL STAINING 1 1 ------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 239 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP I 0 MG/M3 

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 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

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 1526 WITHIN NORMAL LIMITS P P P P 

 1527 WITHIN NORMAL LIMITS P P P P 

 1528 WITHIN NORMAL LIMITS P P P P 

 1530 WITHIN NORMAL LIMITS P P P P 

 1531 WITHIN NORMAL LIMITS P P P P 

 1532 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

 1533 WITHIN NORMAL LIMITS P P P P 

 1534 WITHIN NORMAL LIMITS P P P P 

 1535 WITHIN NORMAL LIMITS P P P P 

 1536 WITHIN NORMAL LIMITS P P P P 

 1537 INCISORS MALOCCLUDED P P P 

 WITHIN NORMAL LIMITS P 

 1538 WITHIN NORMAL LIMITS P P P P 

 1539 WITHIN NORMAL LIMITS P P P P 

 1540 WITHIN NORMAL LIMITS P P P P 

 1541 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 240 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 1542 WITHIN NORMAL LIMITS P P P P 

 1543 WITHIN NORMAL LIMITS P P P P 

 1544 WITHIN NORMAL LIMITS P P P P 

 1545 WITHIN NORMAL LIMITS P P P P 

 1546 WITHIN NORMAL LIMITS P P P P 

 1547 WITHIN NORMAL LIMITS P P P P 

 1549 WITHIN NORMAL LIMITS P P P P 

 1551 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 241 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 INCISORS BROKEN/MISSING P P P P 

 2528 WITHIN NORMAL LIMITS P P P P 

 2529 WITHIN NORMAL LIMITS P P P P 

 2530 WITHIN NORMAL LIMITS P P P P 

 2531 WITHIN NORMAL LIMITS P P P P 

 2532 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 3 3 

 WITHIN NORMAL LIMITS P P 

 2533 WITHIN NORMAL LIMITS P P P P 

 2534 WITHIN NORMAL LIMITS P P P P 

 2535 WITHIN NORMAL LIMITS P P P P 

 2536 WITHIN NORMAL LIMITS P P P P 

 2537 WITHIN NORMAL LIMITS P P P P 

 2539 INCISORS MALOCCLUDED P P 

 WITHIN NORMAL LIMITS P P 

 2540 CHROMODACRYORRHEA - UNILATERAL P P P P 

 INCISORS MALOCCLUDED P P P P 

 2541 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 242 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 2543 WITHIN NORMAL LIMITS P P P P 

 2544 WITHIN NORMAL LIMITS P P P P 

 2545 WITHIN NORMAL LIMITS P P P P 

 2546 WITHIN NORMAL LIMITS P P P P 

 2547 WITHIN NORMAL LIMITS P P P P 

 2548 WITHIN NORMAL LIMITS P P P P 

 2549 WITHIN NORMAL LIMITS P P P P 

 2550 WITHIN NORMAL LIMITS P P P P 

 2551 CHROMODACRYORRHEA - UNILATERAL P 

 INCISORS MALOCCLUDED P 

 WITHIN NORMAL LIMITS P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 243 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

 3527 INCISORS BROKEN/MISSING P 

 WITHIN NORMAL LIMITS P P P 

 3528 WITHIN NORMAL LIMITS P P P P 

 3529 WITHIN NORMAL LIMITS P P P P 

 3530 WITHIN NORMAL LIMITS P P P P 

 3531 WITHIN NORMAL LIMITS P P P P 

 3532 WITHIN NORMAL LIMITS P P P P 

 3533 INCISORS MALOCCLUDED P P P P 

 3534 WITHIN NORMAL LIMITS P P P P 

 3535 WITHIN NORMAL LIMITS P P P P 

 3536 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 3537 WITHIN NORMAL LIMITS P P P P 

 3540 CHROMODACRYORRHEA - UNILATERAL P 

 INCISORS MALOCCLUDED P 

 WITHIN NORMAL LIMITS P P P 

 3541 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 244 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 3542 INCISORS BROKEN/MISSING P 

 WITHIN NORMAL LIMITS P P P 

 3543 INCISORS BROKEN/MISSING P 

 WITHIN NORMAL LIMITS P P P 

 3544 WITHIN NORMAL LIMITS P P P P 

 3545 WITHIN NORMAL LIMITS P P P P 

 3546 WITHIN NORMAL LIMITS P P P P 

 3547 WITHIN NORMAL LIMITS P P P P 

 3548 WITHIN NORMAL LIMITS P P P P 

 3549 WITHIN NORMAL LIMITS P P P P 

 3551 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 245 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 WITHIN NORMAL LIMITS P P P P 

 4527 ALOPECIA - EXTREMITIES/SNOUT 2 2 

 WITHIN NORMAL LIMITS P P 

 4528 WITHIN NORMAL LIMITS P P P P 

 4529 WITHIN NORMAL LIMITS P P P P 

 4530 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

 4531 WITHIN NORMAL LIMITS P P P P 

 4532 WITHIN NORMAL LIMITS P P P P 

 4533 WITHIN NORMAL LIMITS P P P P 

 4534 WITHIN NORMAL LIMITS P P P P 

 4535 WITHIN NORMAL LIMITS P P P P 

 4536 WITHIN NORMAL LIMITS P P P P 

 4537 WITHIN NORMAL LIMITS P P P P 

 4538 WITHIN NORMAL LIMITS P P P P 

 4539 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

 4540 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:00

Huntingdon Life Sciences 00-4206 PAGE 246 

 

 APPENDIX E 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 4542 WITHIN NORMAL LIMITS P P P P 

 4543 ALOPECIA - EXTREMITIES/SNOUT 2 

 INCISORS MALOCCLUDED P P P P 

 INCISORS BROKEN/MISSING P P P P 

 4544 WITHIN NORMAL LIMITS P P P P 

 4545 WITHIN NORMAL LIMITS P P P P 

 4546 WITHIN NORMAL LIMITS P P P P 

 4548 WITHIN NORMAL LIMITS P P P P 

 4550 WITHIN NORMAL LIMITS P P P P 

 4551 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 247 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1526 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1527 FOUND DEAD P 

 1528 WITHIN NORMAL LIMITS P P P P P 

 TERMINAL SACRIFICE P

CHROMODACRYORRHEA - UNILATERAL P 

 INCISORS MALOCCLUDED P

 1529 WITHIN NORMAL LIMITS a P P P P P

 TERMINAL SACRIFICE P

 1530 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1531 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1533 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1534 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1536 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1537 TERMINAL SACRIFICE P 

 INCISORS MALOCCLUDED P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT a

Observations were not performed on Lactation Day 1 due to technician error.
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 248 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1538 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1539 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1540 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1542 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1543 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1544 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1545 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1546 TERMINAL SACRIFICE P 

 INCISORS MALOCCLUDED P P P P P P 

INCISORS BROKEN/MISSING P P P P P P

 1547 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1549 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 249 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1551 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 250 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2526 TERMINAL SACRIFICE P 

 INCISORS BROKEN/MISSING P P P P P P

 2528 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2529 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2530 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2531 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2533 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2534 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2535 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2536 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2537 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 251 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2539 TERMINAL SACRIFICE P 

 INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P

 2540 TERMINAL SACRIFICE P 

 CHROMODACRYORRHEA - UNILATERAL P P P 

 INCISORS MALOCCLUDED P P P P P P

 2541 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2543 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2544 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2545 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2546 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2547 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2548 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2549 WITHIN NORMAL LIMITS P P P P P 

 TERMINAL SACRIFICE P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 252 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2550 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2551 TERMINAL SACRIFICE P 

 CHROMODACRYORRHEA - UNILATERAL P P P P P 

 INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 253 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3527 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 3528 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 3529 TERMINAL SACRIFICE P

 EARS SWOLLEN/RED - BILATERAL P P P P P P

 3530 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3531 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3532 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3533 TERMINAL SACRIFICE P 

 INCISORS MALOCCLUDED P P P P P P

 3534 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3535 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3536 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 INCISORS BROKEN/MISSING P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 254 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3537 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 3540 TERMINAL SACRIFICE P 

 CHROMODACRYORRHEA - UNILATERAL P P P P P P

 INCISORS MALOCCLUDED P P P P P P

 3541 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3542 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3543 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3544 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3545 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3546 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3547 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3548 WITHIN NORMAL LIMITS P P P P P 

 TERMINAL SACRIFICE P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 255 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3549 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 3551 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 256 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4526 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4527 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4528 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4529 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4531 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4532 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4533 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4534 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4535 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4536 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 257 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4537 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4538 WITHIN NORMAL LIMITS P P 

 TERMINAL SACRIFICE P

INCISORS BROKEN/MISSING P P P P

 4540 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4542 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4543 TERMINAL SACRIFICE P 

 INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P P

 4544 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4545 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4546 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4547 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4548 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:01

Huntingdon Life Sciences 00-4206 PAGE 258 

 

 APPENDIX F 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4550 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4551 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 259 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 1026 135 194 250 300 340 368 391 414 434 456 472 489 494 507 511 516 

 1027 107 169 226 279 325 352 378 393 415 428 454 464 470 483 496 500 

 1028 117 189 242 293 332 361 383 405 429 438 443 461 476 491 515 516 

 1029 121 193 251 297 348 377 413 439 465 475 484 501 504 518 521 529 

 1030 121 190 250 304 358 386 416 449 474 493 516 533 545 565 575 584 

 1031 105 168 222 282 332 358 385 415 439 451 468 477 488 496 497 503 

 1032 124 190 239 290 323 346 369 390 418 428 444 469 477 492 499 502 

 1033 122 203 269 345 412 455 506 537 574 596 614 642 647 674 668 693 

 1034 110 179 237 300 358 393 439 460 481 501 527 539 564 578 587 603 

 1035 128 198 245 305 348 373 405 424 438 441 461 479 489 504 515 551 

 1036 112 179 228 284 339 371 411 430 450 465 490 488 496 512 518 526 

 1037 115 187 243 310 362 397 426 450 481 490 504 518 518 528 541 568 

 1038 117 191 256 323 383 409 438 466 496 499 509 517 540 554 563 581 

 1039 108 178 228 290 335 356 392 417 439 453 470 484 502 514 526 541 

 1040 114 186 250 319 379 420 449 473 500 518 535 550 574 588 601 624 

 1041 104 169 221 276 318 355 389 410 431 451 462 484 505 512 531 549 

 1042 125 195 251 303 352 384 424 446 476 491 506 521 537 548 564 574 

 1043 130 194 252 299 344 374 411 428 456 464 477 495 505 519 535 544 

 1044 112 186 243 295 347 372 410 430 443 450 466 478 488 501 514 531 

 1045 109 174 219 268 303 313 332 343 360 375 384 385 396 408 423 433 

 1046 108 173 225 280 333 365 395 418 443 458 478 486 505 518 526 529 

 1047 113 181 244 316 372 405 437 467 484 502 528 535 538 556 568 576 

 1048 104 170 223 282 326 349 372 388 401 418 434 434 436 436 445 458 

 1049 105 169 217 283 334 369 398 426 447 444 457 470 492 508 525 538 

 1050 115 187 254 312 360 391 417 444 468 480 494 510 522 524 539 550 

 1051 133 209 273 338 388 411 442 466 484 503 517 535 543 559 574 583 

MEAN 116 185 241 299 348 377 409 432 455 468 484 498 510 523 534 546 

S.D. 9.1 11.2 15.2 18.8 24.4 28.5 33.2 36.6 39.8 41.4 43.1 46.2 47.1 50.1 48.2 51.6

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
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Huntingdon Life Sciences 00-4206 PAGE 260 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 2026 131 200 259 314 358 404 439 470 489 506 525 525 530 548 562 574 

 2027 112 175 223 281 335 372 410 438 466 475 492 509 525 535 546 552 

 2028 118 189 246 309 356 397 432 451 484 504 521 530 532 545 555 566 

 2029 109 179 236 297 349 391 436 471 495 517 544 556 579 592 610 618 

 2030 127 201 255 317 366 407 439 468 497 515 538 556 562 567 585 596 

 2031 125 195 251 310 355 389 420 443 468 481 498 507 515 527 539 555 

 2032 120 190 246 298 346 379 400 436 457 482 500 512 536 552 568 576 

 2033 125 190 255 321 365 400 437 473 492 512 530 551 567 589 599 619 

 2034 112 171 224 283 328 351 391 416 434 455 473 490 500 512 525 533 

 2035 105 171 226 285 330 364 399 421 439 454 472 481 492 506 510 521 

 2036 132 209 268 339 384 416 441 463 479 496 517 536 538 554 560 574 

 2037 109 174 234 290 340 363 397 421 449 464 482 493 506 522 529 529 

 2038 108 173 229 279 317 350 369 399 415 429 454 461 478 497 507 515 

 2039 121 192 253 314 363 401 434 465 480 506 516 528 533 561 575 582 

 2040 106 168 215 261 302 337 367 387 413 430 447 459 455 484 499 516 

 2041 135 204 262 315 364 400 440 457 477 495 514 512 531 535 545 564 

 2042 115 186 253 318 381 423 460 490 519 534 551 575 581 592 606 619 

 2043 106 172 223 279 334 361 396 419 443 452 458 469 484 501 516 526 

 2044 104 168 219 267 303 317 344 364 381 400 420 429 446 457 469 477 

 2045 122 190 249 305 353 381 413 422 446 455 472 481 486 490 506 517 

 2046 116 189 247 313 368 399 430 455 471 484 498 513 524 531 538 557 

 2047 114 183 240 300 340 368 411 430 456 472 491 498 513 517 523 532 

 2048 117 187 239 297 338 368 396 406 425 432 445 451 467 472 473 487 

 2049 114 176 229 277 325 357 388 411 440 448 461 480 488 498 500 519 

 2050 103 160 218 282 322 361 392 412 428 442 470 482 505 518 523 529 

 2051 111 177 231 291 327 358 389 414 431 445 464 490 495 524 535 541 

MEAN 116 183 240 298 344 377 410 435 457 472 491 503 514 528 539 550

S.D. 9.1 12.6 15.1 18.8 21.8 25.7 27.8 30.4 31.7 33.6 34.1 35.6 35.4 35.7 37.6 38.1 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 261 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 3026 104 165 209 255 286 314 333 347 366 383 389 401 419 429 436 447 

 3027 115 182 237 295 339 358 395 420 428 446 460 474 494 512 507 512 

 3028 117 186 240 307 353 385 420 444 470 496 524 544 562 569 571 591 

 3029 118 185 250 304 355 384 416 434 457 480 492 502 517 533 540 541 

 3030 104 166 223 280 331 370 406 443 468 486 510 517 545 560 575 581 

 3031 118 187 237 280 319 343 373 393 410 428 441 453 464 473 481 475 

 3032 106 171 215 269 310 336 359 380 394 413 425 436 439 445 459 468 

 3033 108 175 240 288 329 360 387 405 426 439 446 451 461 470 479 478 

 3034 113 175 231 283 335 363 398 424 443 462 482 498 516 532 545 553 

 3035 131 188 239 289 321 344 367 391 402 415 431 437 448 449 458 461 

 3036 123 191 249 307 352 385 413 436 457 479 497 511 519 533 537 548 

 3037 113 174 230 280 319 347 363 372 393 400 419 414 425 423 425 433 

 3038 137 207 262 314 360 383 410 425 460 472 501 516 526 535 540 553 

 3039 106 166 225 260 300 346 385 407 431 442 461 473 485 494 503 512 

 3040 109 172 228 285 328 367 403 433 455 477 502 506 530 542 551 559 

 3041 127 202 270 331 376 402 437 455 476 496 528 543 563 571 580 591 

 3042 103 162 211 257 302 341 368 385 405 424 437 450 463 475 487 497 

 3043 112 170 226 283 319 335 360 379 401 413 421 436 441 454 469 471 

 3044 116 186 243 308 363 387 413 441 470 472 496 513 529 549 560 563 

 3045 110 175 241 294 345 380 412 428 449 462 469 483 497 493 512 524 

 3046 110 179 238 300 362 399 441 468 492 517 534 538 518 570 588 602 

 3047 126 189 246 295 335 358 386 408 430 454 474 493 499 508 519 519 

 3048 122 194 255 326 377 412 435 451 479 501 514 534 507 559 571 582 

 3049 131 194 249 303 341 372 398 411 433 452 468 484 489 502 515 526 

 3050 123 197 263 325 371 407 438 460 483 497 512 524 529 526 541 556 

 3051 114 186 248 314 369 407 435 455 492 505 525 543 560 577 588 586 

MEAN 116 182 239 294 338 369 398 419 441 458 475 487 498 511 521 528 

S.D. 9.2 11.9 15.5 20.5 24.8 25.8 28.8 31.0 34.3 35.9 39.9 42.1 42.2 47.1 47.6 49.4

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 262 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 4026 112 178 234 287 328 362 391 428 433 447 465 470 487 503 494 507 

 4027 138 199 234 281 317 345 360 373 402 421 430 444 455 453 454 458 

 4028 109 181 239 295 328 364 394 419 441 463 483 492 481 500 515 532 

 4029 121 194 254 312 364 394 429 452 478 486 495 510 520 532 542 547 

 4030 114 181 234 287 324 357 383 418 431 448 470 488 502 513 520 512 

 4031 116 179 223 272 315 346 362 391 410 421 443 453 469 475 482 477 

 4032 102 162 215 266 314 352 384 413 437 454 468 479 485 483 504 525 

 4033 115 182 236 286 331 367 388 415 440 454 474 485 506 526 541 522 

 4034 108 172 220 265 300 330 356 383 387 403 421 427 439 445 453 471 

 4035 130 198 247 310 358 391 411 436 462 480 497 507 514 529 552 566 

 4052 101 166 213 269 307 331 367 414 424 433 452 461 476 490 493 498 

 4037 128 192 243 283 317 342 362 378 401 405 410 425 444 446 447 448 

 4038 123 191 243 301 345 381 408 434 456 464 485 485 503 507 511 523 

 4039 110 178 234 282 331 361 388 409 434 446 455 462 470 483 491 500 

 4040 106 169 218 264 307 336 362 380 398 415 427 437 448 454 460 470 

 4041 123 186 231 288 332 370 402 450 448 470 493 497 510 522 527 536 

 4042 132 193 243 286 323 344 372 406 417 428 440 443 446 451 461 464 

 4043 113 174 221 280 324 352 386 398 410 427 445 454 474 486 495 504 

 4044 112 179 234 286 335 364 400 432 459 483 505 521 528 538 554 554 

 4045 115 182 234 293 344 377 408 439 478 476 494 510 508 531 537 553 

 4046 106 172 225 281 333 357 378 407 416 431 451 459 462 460 471 478 

 4047 118 180 229 279 328 360 389 421 429 444 464 458 472 483 483 497 

 4048 110 176 230 279 328 368 387 405 424 441 454 458 458 463 476 483 

 4049 120 183 232 278 309 336 351 370 381 390 396 391 409 417 429 438 

 4050 104 170 228 279 315 340 371 422 428 430 441 456 482 478 495 488 

 4051 126 190 248 303 352 381 412 442 462 482 497 505 517 529 545 556 

MEAN 116 181 232 284 327 358 385 413 430 444 460 468 479 488 497 504

S.D. 9.5 9.8 10.4 12.6 15.8 17.8 20.1 23.3 25.9 26.6 29.2 31.2 29.5 33.3 35.3 35.8 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 263 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 1026 527 543 

 1027 500 516 

 1028 536 549 

 1029 541 555 

 1030 594 613 

 1031 520 522 

 1032 509 520 

 1033 712 742 

 1034 600 610 

 1035 562 566 

 1036 533 541 

 1037 567 581 

 1038 586 593 

 1039 542 546 

 1040 637 659 

 1041 557 566 

 1042 585 594 

 1043 558 574 

 1044 539 547 

 1045 444 449 

 1046 529 540 

 1047 585 600 

 1048 470 477 

 1049 550 562 

 1050 558 563 

 1051 591 613 

MEAN 555 567

S.D. 52.2 56.8

 N 26 26
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 264 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 2026 588 600 

 2027 570 587 

 2028 572 587 

 2029 636 643 

 2030 611 612 

 2031 555 561 

 2032 605 599 

 2033 637 658 

 2034 557 568 

 2035 541 551 

 2036 584 601 

 2037 545 553 

 2038 528 537 

 2039 590 603 

 2040 518 528 

 2041 566 574 

 2042 637 648 

 2043 538 551 

 2044 493 491 

 2045 531 535 

 2046 583 588 

 2047 543 564 

 2048 506 514 

 2049 530 538 

 2050 539 545 

 2051 537 548 

MEAN 563 573

S.D. 39.7 41.1

 N 26 26
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 265 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 3026 461 471 

 3027 537 553 

 3028 605 629 

 3029 557 571 

 3030 598 614 

 3031 486 499 

 3032 485 495 

 3033 490 496 

 3034 566 584 

 3035 463 477 

 3036 557 561 

 3037 452 463 

 3038 565 575 

 3039 524 528 

 3040 568 583 

 3041 599 625 

 3042 509 519 

 3043 477 492 

 3044 577 596 

 3045 525 545 

 3046 617 629 

 3047 534 544 

 3048 595 597 

 3049 539 546 

 3050 569 587 

 3051 596 602 

MEAN 540 553

S.D. 49.7 51.6

 N 26 26
23-JUN-2009 18:04

Huntingdon Life Sciences 00-4206 PAGE 266 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 4026 536 550 

 4027 480 498 

 4028 544 562 

 4029 554 564 

 4030 534 554 

 4031 495 515 

 4032 540 557 

 4033 538 556 

 4034 474 486 

 4035 580 582 

 4052 513 522 

 4037 480 499 

 4038 531 545 

 4039 520 532 

 4040 481 493 

 4041 545 549 

 4042 474 488 

 4043 515 526 

 4044 572 590 

 4045 569 581 

 4046 491 506 

 4047 506 514 

 4048 498 508 

 4049 439 451 

 4050 500 512 

 4051 562 580 

MEAN 518 531

S.D. 35.9 35.7

 N 26 26
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 267 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 1526 121 155 187 210 223 243 256 263 266 279 277 272 

 1527 144 182 208 236 256 277 291 297 304 320 322 318 

 1528 130 179 204 224 254 267 280 282 297 308 299 316 

 1529 145 181 200 232 235 255 264 284 301 300 304 305 327 367 397 495 

 1530 139 189 211 235 253 258 280 289 297 309 327 312 

 1531 137 172 208 225 249 266 283 280 300 298 329 332 

 1532 134 165 190 214 234 251 257 264 275 277 278 283 

 1533 128 167 193 210 229 243 257 261 267 270 276 277 

 1534 132 164 191 211 230 242 250 253 264 272 277 281 

 1535 130 165 181 211 232 237 244 261 266 266 273 268 

 1536 132 167 188 207 218 235 251 255 256 262 271 269 

 1537 127 163 192 209 235 242 251 251 266 265 270 271 

 1538 128 171 198 222 239 246 267 272 279 284 297 300 

 1539 120 157 181 208 222 239 257 269 268 277 277 284 

 1540 125 164 186 217 238 249 255 273 281 287 283 293 

 1541 122 157 178 204 221 220 237 248 255 252 265 260 

 1542 149 191 220 247 268 272 283 297 315 313 315 322 

 1543 123 160 191 216 234 244 255 264 274 284 286 297 

 1544 133 178 208 239 256 276 301 313 324 329 340 330 

 1545 142 182 214 232 264 273 292 293 316 327 323 341 

 1546 135 162 182 187 209 219 227 225 241 245 248 254 

 1547 123 155 183 201 215 220 231 236 252 256 256 264 

 1548 148 182 217 239 258 270 278 294 305 306 313 302 331 335 319 318 

 1549 135 173 196 226 241 244 265 265 282 284 292 292 

 1550 118 155 170 193 211 224 236 239 254 261 257 270 274 269 268 281 

 1551 122 161 176 207 227 238 252 245 267 264 277 268 

MEAN 132 169 194 218 237 248 261 268 280 284 290 291 311 324 328 364 

S.D. 9.0 10.9 13.7 15.1 16.5 17.7 19.6 21.5 22.6 23.7 25.0 24.6 32.1 49.8 64.9 114.4

 N 26 26 26 26 26 26 26 26 26 26 26 26 3 3 3 3
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 268 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 2526 126 164 192 196 219 232 244 247 264 274 277 276 

 2527 136 170 191 221 244 253 255 267 276 273 272 278 278 289 294 308 

 2528 149 178 209 228 251 256 272 277 287 282 283 288 

 2529 137 172 204 229 246 272 289 297 295 304 307 301 

 2530 149 177 208 237 260 258 285 291 306 315 308 327 332 

 2531 124 154 189 209 219 238 252 265 261 266 297 312 

 2532 123 157 180 198 217 230 244 254 257 265 287 278 

 2533 128 161 188 210 234 247 250 267 280 284 284 295 

 2534 129 159 181 209 219 237 246 259 275 276 273 280 

 2535 132 165 193 222 232 240 256 268 275 295 321 306 

 2536 122 161 195 216 240 254 273 278 295 296 299 300 

 2537 122 154 181 194 218 231 240 246 253 260 253 255 

 2538 130 176 194 227 246 252 267 271 284 303 295 285 287 315 304 310 

 2539 134 167 195 217 234 242 259 268 279 282 286 288 

 2540 117 149 174 200 210 229 242 250 261 263 274 267 

 2541 144 191 231 270 290 313 324 340 358 347 358 364 

 2542 135 171 192 210 228 239 241 258 265 274 277 277 295 297 294 306 

 2543 144 184 212 233 244 260 284 292 309 324 324 333 

 2544 132 182 194 209 237 246 271 275 286 286 299 296 

 2545 133 167 191 214 232 242 258 264 271 277 283 285 

 2546 120 162 171 199 215 228 229 247 250 255 253 264 

 2547 120 146 171 189 205 212 224 234 237 238 248 248 

 2548 124 159 186 209 227 242 256 262 270 281 300 317 

 2549 130 163 191 206 233 245 259 279 278 290 305 296 348a

 

 2550 139 175 199 227 238 245 259 269 274 275 282 280 

 2551 141 176 203 222 246 265 280 284 296 307 315 318 

MEAN 132 167 193 215 234 246 260 270 278 284 291 293 298 300 297 318

S.D. 9.0 10.9 13.4 16.8 17.7 18.8 21.2 21.0 23.6 23.1 24.1 25.3 23.9 13.5 5.7 20.2 

 N 26 26 26 26 26 26 26 26 26 26 26 26 4 3 3 4

a

Animal was pregnant but had total litter loss. Recording of body weights was resumed.
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 269 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 3526 135 173 204 219 242 256 255 272 279 288 283 293 

 3527 140 178 208 224 246 264 276 273 295 303 308 312 

 3528 138 166 184 207 216 222 236 238 256 265 280 273 

 3529 133 166 189 210 216 235 244 251 250 259 266 260 

 3530 118 157 184 194 224 236 247 257 274 264 275 290 

 3531 130 160 183 195 220 233 244 246 257 262 267 268 

 3532 148 178 202 225 241 256 262 271 282 280 294 301 

 3533 142 181 217 247 272 273 294 300 300 306 312 309 

 3534 136 169 195 206 227 242 246 248 260 262 260 271 

 3535 121 160 188 206 216 237 251 268 258 269 275 299 

 3536 124 173 191 218 239 247 255 271 282 284 281 295 295a

 

 3537 129 158 175 203 218 231 229 243 255 252 279 291 

 3538 130 167 200 222 240 255 264 273 282 280 304 311 300 305 293 307 

 3539 126 160 190 213 222 248 262 272 273 288 296 291 320 346 335 327 

 3540 122 154 181 198 218 237 248 258 268 274 277 281 

 3541 117 164 186 212 226 238 250 253 263 275 289 277 

 3542 149 189 219 238 257 272 284 292 294 308 320 316 

 3543 125 159 178 198 215 218 233 238 243 262 275 269 

 3544 123 157 181 201 212 222 231 244 246 256 260 256 

 3545 121 150 181 199 221 228 246 244 261 272 286 272 

 3546 134 161 194 207 235 244 245 259 259 268 282 306 

 3547 127 153 177 183 209 207 226 239 247 244 256 259 

 3548 140 179 215 245 257 282 291 300 296 314 330 310 353a

 

 3549 131 164 196 220 234 246 243 264 269 266 273 273 

 3550 144 180 220 240 259 267 276 291 295 301 321 337 346 362 338 345 

 3551 133 169 210 221 248 249 268 270 288 300 294 303 

MEAN 131 166 194 213 232 244 254 263 270 277 286 289 322 338 322 325

S.D. 9.1 10.1 13.8 16.3 16.9 18.1 18.6 18.8 17.5 19.1 19.8 20.6 22.9 29.7 25.5 24.5 

 N 26 26 26 26 26 26 26 26 26 26 26 26 3 3 3 5

a

Animal was pregnant but had total litter loss. Recording of body weights was resumed.
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 270 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 4526 130 157 179 199 223 238 255 259 271 279 277 272 

 4527 134 166 200 224 247 265 267 284 296 308 297 307 

 4528 123 157 178 207 228 238 259 273 280 278 288 283 

 4529 131 175 184 207 224 240 248 263 271 269 278 271 

 4530 134 169 182 200 201 229 235 234 252 262 260 263 

 4531 123 154 165 191 210 218 225 250 244 247 247 248 

 4532 131 159 174 184 211 215 220 241 249 246 249 261 

 4533 149 178 194 200 220 235 250 261 266 266 277 278 

 4534 147 182 201 224 240 250 269 279 284 276 290 287 

 4535 118 154 168 186 198 209 218 223 231 232 240 242 

 4536 134 162 172 207 222 235 241 260 260 266 259 275 

 4537 137 170 181 210 214 242 246 262 264 272 278 279 

 4538 121 153 180 200 213 230 240 254 249 259 270 262 

 4539 125 154 179 191 212 215 234 242 244 254 267 251 

 4540 126 153 168 191 210 211 230 237 249 252 248 257 

 4541 144 181 213 234 237 259 275 285 278 287 299 298 304 308 308 311 

 4542 118 160 185 213 217 242 248 259 269 261 279 283 

 4543 129 174 195 211 235 251 245 273 267 281 285 296 

 4544 124 160 175 202 217 227 232 244 249 250 250 258 

 4545 145 179 201 221 233 247 253 255 270 279 275 277 

 4546 139 178 191 224 242 251 251 275 283 286 281 299 

 4547 127 168 182 211 230 247 281 271 262 276 287 278 308 329 378 

 4548 135 178 204 216 244 259 269 273 287 292 294 309 

 4549 140 165 193 204 220 216 235 

 4550 132 159 190 208 233 243 254 262 269 275 278 287 300 

 4551 122 158 185 201 215 237 250 256 257 267 266 265 

MEAN 131 165 185 206 223 236 247 259 264 269 273 275 304 318 343 311 

S.D. 8.9 9.8 12.2 12.6 13.2 15.5 16.4 15.9 15.7 16.8 16.8 18.1 3.9 15.3 49.9 0.0 

 N 26 26 26 26 26 26 26 25 25 25 25 25 3 2 2 1
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 271 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

 1548 334 

 1550 285 

MEAN 310 

S.D. 34.6 

 N 2 
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 272 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

 2527 315 

 2538 318 

 2542 316 

 2549 349 354 351 342 

MEAN 324 354 351 342

S.D. 16.6 0.0 0.0 0.0

 N 4 1 1 1
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 273 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

 3536 298 318 314 322 

 3538 312 

 3539 324 

 3548 364 340 348 339 

 3550 351 

MEAN 330 329 331 331

S.D. 27.4 15.7 24.0 11.6

 N 5 2 2 2
23-JUN-2009 18:05

Huntingdon Life Sciences 00-4206 PAGE 274 

 

 APPENDIX G 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

 4541 325 

MEAN 325 

S.D. 0.0 

 N 1 
23-JUN-2009 18:12

Huntingdon Life Sciences 00-4206 PAGE 275 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 59 56 51 40 28 23 22 20 23 16 17 6 12 4 5 12 

 1027 63 56 54 45 27 26 15 22 14 25 10 6 13 13 4 0 

 1028 71 53 51 39 28 22 22 25 9 5 19 15 15 24 1 19 

 1029 72 57 47 51 29 36 27 26 10 9 17 4 13 3 8 12 

 1030 69 60 55 54 28 30 33 25 19 23 17 12 19 10 9 11 

 1031 63 54 60 49 27 27 30 24 12 16 10 10 8 2 6 17 

 1032 66 49 51 33 23 24 21 28 10 16 25 7 15 7 3 8 

 1033 81 66 76 67 43 51 31 37 22 19 28 5 28 -7 25 20 

 1034 69 58 64 58 35 46 21 21 20 26 12 25 14 10 16 -4 

 1035 70 47 60 43 24 32 19 14 3 20 18 10 15 11 36 10 

 1036 67 49 57 55 31 40 19 20 15 25 -3 9 15 6 8 7 

 1037 72 56 66 52 36 29 24 31 9 14 14 1 9 14 26 0 

 1038 75 65 67 60 26 29 28 30 3 10 8 22 15 8 18 5 

 1039 70 50 61 46 21 36 25 22 14 17 14 18 12 12 15 1 

 1040 72 64 69 59 41 29 24 27 18 17 16 24 13 13 23 13 

 1041 65 52 55 43 36 35 21 21 20 11 22 21 7 19 18 8 

 1042 71 56 52 49 32 40 22 30 15 15 15 17 11 16 10 11 

 1043 64 59 47 44 31 37 17 29 8 13 18 10 14 16 9 14 

 1044 74 57 52 52 25 37 21 12 7 16 13 10 13 13 17 8 

 1045 64 45 50 35 10 19 11 17 15 9 1 11 12 15 11 11 

 1046 64 52 56 53 32 29 23 24 16 20 7 19 14 8 3 1 

 1047 67 64 71 56 34 32 30 17 18 26 7 3 18 12 8 9 

 1048 66 53 59 44 23 23 16 13 17 17 -1 2 1 9 13 12 

 1049 64 48 65 51 35 29 28 21 -4 14 13 23 16 17 13 12 

 1050 73 67 58 48 30 26 28 23 13 14 16 12 1 15 11 8 

 1051 76 64 65 50 23 31 24 18 19 14 19 8 16 15 9 8 

MEAN 69 56 58 49 29 32 23 23 13 16 13 12 13 11 13 9 

S.D. 4.9 6.3 7.8 7.9 6.8 7.4 5.3 5.9 6.4 5.6 7.2 7.2 5.3 6.2 8.3 5.9

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
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 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 70 59 55 44 46 35 31 19 17 18 1 4 19 13 12 14 

 2027 62 49 58 54 37 38 29 27 9 18 16 16 10 11 6 19 

 2028 71 57 63 47 41 35 19 33 20 17 9 2 13 11 11 6 

 2029 69 58 61 52 42 45 35 24 22 27 12 23 13 18 8 19 

 2030 73 55 61 50 40 33 29 29 18 23 18 7 5 18 11 16 

 2031 70 56 59 45 35 31 22 25 13 17 9 8 12 11 16 0 

 2032 70 55 53 47 33 21 35 21 26 18 12 24 16 15 9 29 

 2033 65 65 66 44 36 36 37 19 20 18 20 17 22 10 20 17 

 2034 59 53 59 45 24 40 25 18 21 18 17 10 12 13 8 25 

 2035 66 55 60 45 34 36 22 19 14 18 10 11 14 4 11 20 

 2036 77 59 72 45 32 25 22 16 17 22 18 2 17 6 13 11 

 2037 65 60 57 50 22 35 24 28 15 18 11 14 15 8 0 16 

 2038 65 55 51 37 34 19 30 16 13 25 7 18 19 10 8 13 

 2039 71 61 61 49 38 33 31 15 26 11 11 5 29 14 7 8 

 2040 63 47 46 40 35 30 21 26 17 18 12 -4 29 16 17 1 

 2041 69 58 53 49 36 40 18 20 18 19 -2 18 5 10 19 2 

 2042 71 67 65 63 42 37 29 29 15 17 24 6 12 14 13 18 

 2043 66 51 57 55 27 35 23 24 9 6 11 15 18 14 10 13 

 2044 64 51 48 36 14 27 20 17 19 20 10 17 11 12 9 15 

 2045 68 59 57 47 28 32 9 25 9 17 9 6 4 16 11 14 

 2046 73 59 65 55 31 31 25 17 12 14 15 11 7 7 19 26 

 2047 69 57 60 40 29 43 19 26 16 19 7 15 3 6 9 11 

 2048 70 53 58 40 31 28 10 18 7 13 7 16 5 1 14 18 

 2049 62 53 48 48 32 31 23 28 9 13 18 9 10 2 19 11 

 2050 56 58 64 40 39 31 19 17 13 29 11 24 13 5 6 10 

 2051 66 54 60 36 31 31 25 18 14 19 25 6 29 11 6 -4 

MEAN 67 56 58 46 33 33 24 22 16 18 12 11 14 11 11 13

S.D. 4.6 4.5 6.0 6.5 6.9 6.0 6.9 5.1 5.0 4.8 6.3 7.3 7.4 4.5 5.0 7.8 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
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Huntingdon Life Sciences 00-4206 PAGE 277 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 61 44 46 31 28 19 13 19 17 6 12 19 10 8 11 14 

 3027 67 55 58 43 19 37 25 8 18 14 14 20 18 -5 5 25 

 3028 69 54 68 46 32 35 25 26 26 28 20 18 7 2 20 14 

 3029 66 65 54 52 29 32 18 23 23 12 11 14 17 6 2 16 

 3030 62 56 58 51 39 37 37 25 18 24 7 29 15 15 7 17 

 3031 69 50 43 38 25 30 20 17 18 12 12 11 9 8 -6 11 

 3032 65 45 54 41 25 23 21 14 20 11 11 3 6 14 9 17 

 3033 68 65 48 41 31 28 18 21 12 7 5 10 9 10 -1 12 

 3034 62 57 52 52 29 34 27 19 19 20 16 19 15 13 8 13 

 3035 57 51 51 32 22 24 24 11 13 16 6 11 1 10 3 2 

 3036 68 58 58 45 33 29 23 21 23 18 14 8 14 4 11 9 

 3037 61 57 50 39 28 16 9 21 7 19 -5 11 -2 2 8 19 

 3038 71 55 52 46 23 27 14 36 11 29 15 10 10 4 13 12 

 3039 60 59 35 40 46 39 23 23 12 19 12 12 9 10 9 13 

 3040 63 56 57 44 38 36 30 22 21 25 5 24 13 9 8 9 

 3041 75 68 61 46 26 35 18 21 20 32 15 19 8 9 12 8 

 3042 59 49 46 45 39 27 17 20 19 13 12 13 12 13 10 12 

 3043 58 55 57 36 17 24 19 22 13 8 14 5 13 15 2 6 

 3044 71 56 66 55 23 26 28 29 3 24 17 16 20 12 3 13 

 3045 66 66 52 52 34 32 16 21 13 7 13 15 -4 19 12 1 

 3046 69 60 62 62 37 43 27 24 25 17 5 -21 52 18 15 15 

 3047 62 57 50 40 23 28 22 22 24 20 19 6 9 11 1 15 

 3048 72 62 70 52 34 24 16 28 22 13 19 -27 53 12 11 14 

 3049 62 56 54 38 31 26 13 22 19 17 15 5 13 13 11 13 

 3050 74 66 62 46 35 32 22 23 14 15 12 5 -4 16 15 13 

 3051 72 62 66 54 39 28 20 37 13 20 18 17 17 11 -2 10 

MEAN 66 57 55 45 30 30 21 22 17 17 12 10 13 10 7 12 

S.D. 5.1 6.2 8.1 7.3 7.2 6.3 6.0 6.2 5.7 6.9 5.6 11.8 13.2 5.3 5.9 4.9

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
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Huntingdon Life Sciences 00-4206 PAGE 278 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 65 56 53 41 33 29 37 5 15 17 5 18 16 -10 14 29 

 4027 62 35 47 37 28 15 14 29 19 9 14 11 -2 0 4 22 

 4028 72 58 55 33 36 30 25 22 22 20 9 -11 19 15 18 12 

 4029 73 60 58 51 30 35 23 25 9 9 15 10 12 10 5 7 

 4030 67 53 53 37 33 26 36 13 17 22 18 13 11 7 -8 22 

 4031 62 45 49 43 31 17 29 19 11 22 10 17 5 8 -5 18 

 4032 60 53 52 48 37 32 29 24 17 14 11 5 -1 21 21 15 

 4033 67 54 50 45 36 21 27 25 15 19 11 21 21 15 -20 16 

 4034 65 48 45 35 30 26 27 4 16 18 6 12 6 8 18 3 

 4035 68 48 64 48 33 20 24 26 18 17 10 7 15 23 15 13 

 4052 64 47 56 38 25 36 47 10 9 19 9 15 14 3 5 15 

 4037 64 51 40 35 25 20 17 23 3 5 15 19 2 1 1 32 

 4038 68 52 58 44 37 26 26 22 8 21 0 18 4 4 11 8 

 4039 68 56 48 49 30 27 21 25 13 9 7 8 13 8 10 20 

 4040 63 49 46 43 29 26 19 18 17 12 9 12 5 6 10 11 

 4041 63 45 58 43 38 33 48 -2 22 23 4 14 11 5 10 9 

 4042 62 49 43 37 22 28 34 10 11 12 3 4 5 10 3 10 

 4043 61 46 59 44 28 35 12 12 17 18 9 20 12 8 9 11 

 4044 68 55 52 50 29 36 32 27 25 21 16 7 10 16 -1 19 

 4045 67 51 59 51 33 32 31 38 -2 18 17 -2 23 6 16 16 

 4046 67 53 56 52 24 21 29 9 16 19 9 2 -1 10 7 13 

 4047 62 48 50 49 32 29 32 8 15 20 -6 15 11 0 15 8 

 4048 66 54 50 49 40 20 18 19 17 13 4 0 5 13 7 15 

 4049 63 49 46 30 27 16 18 11 10 5 -4 18 8 12 8 1 

 4050 66 58 51 36 26 30 51 6 2 11 15 26 -4 17 -7 12 

 4051 64 58 55 49 30 31 29 21 19 15 9 12 12 16 11 6 

MEAN 65 51 52 43 31 27 28 17 14 16 9 11 9 9 7 14

S.D. 3.3 5.4 5.7 6.5 4.7 6.4 9.9 9.4 6.3 5.3 6.1 8.2 7.0 7.1 9.2 7.2 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
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Huntingdon Life Sciences 00-4206 PAGE 279 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 16 295 350 

 1027 16 295 347 

 1028 13 273 360 

 1029 14 307 362 

 1030 19 343 423 

 1031 2 309 354 

 1032 10 280 330 

 1033 30 439 540 

 1034 10 360 431 

 1035 4 281 368 

 1036 7 309 362 

 1037 14 330 394 

 1038 8 326 402 

 1039 3 306 368 

 1040 22 365 474 

 1041 9 315 397 

 1042 10 325 399 

 1043 16 302 381 

 1044 8 292 361 

 1045 5 212 275 

 1046 10 313 367 

 1047 14 354 419 

 1048 7 264 307 

 1049 12 301 392 

 1050 5 323 375 

 1051 22 326 404 

MEAN 12 313 382

S.D. 6.6 41.1 51.3

 N 26 26 26
23-JUN-2009 18:12

Huntingdon Life Sciences 00-4206 PAGE 280 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 12 325 400 

 2027 17 334 412 

 2028 15 341 398 

 2029 7 378 464 

 2030 1 355 411 

 2031 6 313 366 

 2032 -6 322 409 

 2033 22 360 468 

 2034 11 319 397 

 2035 10 310 380 

 2036 17 327 392 

 2037 8 319 379 

 2038 9 287 364 

 2039 14 335 411 

 2040 11 291 360 

 2041 8 308 370 

 2042 11 389 462 

 2043 13 297 379 

 2044 -2 262 323 

 2045 4 291 345 

 2046 5 325 400 

 2047 21 315 381 

 2048 9 264 328 

 2049 9 304 363 

 2050 6 322 385 

 2051 11 313 371 

MEAN 9 319 389

S.D. 6.3 29.9 36.5

 N 26 26 26
23-JUN-2009 18:12

Huntingdon Life Sciences 00-4206 PAGE 281 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 11 236 307 

 3027 16 292 371 

 3028 25 358 444 

 3029 14 318 386 

 3030 16 350 447 

 3031 13 266 313 

 3032 10 265 324 

 3033 6 276 321 

 3034 18 323 409 

 3035 14 249 289 

 3036 4 320 370 

 3037 11 240 289 

 3038 10 308 368 

 3039 3 307 362 

 3040 16 334 411 

 3041 26 342 423 

 3042 10 288 357 

 3043 14 265 321 

 3044 20 327 410 

 3045 20 307 369 

 3046 12 360 451 

 3047 10 304 355 

 3048 1 340 403 

 3049 8 290 353 

 3050 18 327 390 

 3051 6 357 416 

MEAN 13 306 371

S.D. 6.2 36.9 47.9

 N 26 26 26
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Huntingdon Life Sciences 00-4206 PAGE 282 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 14 292 372 

 4027 18 245 299 

 4028 18 310 381 

 4029 10 316 370 

 4030 20 307 372 

 4031 20 274 336 

 4032 17 318 395 

 4033 19 303 375 

 4034 12 255 314 

 4035 3 309 384 

 4052 9 296 357 

 4037 19 234 307 

 4038 14 294 354 

 4039 12 284 354 

 4040 12 268 324 

 4041 5 311 364 

 4042 15 249 295 

 4043 11 280 351 

 4044 18 342 411 

 4045 13 328 399 

 4046 15 287 334 

 4047 8 277 333 

 4048 10 282 332 

 4049 12 209 268 

 4050 12 285 342 

 4051 18 315 390 

MEAN 13 287 350

S.D. 4.6 30.7 35.5

 N 26 26 26
23-JUN-2009 18:25

Huntingdon Life Sciences 00-4206 PAGE 283 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 34 32 24 12 21 13 8 2 13 -2 -5 

 1527 38 26 28 20 21 14 6 7 15 2 -5 

 1528 49 25 20 30 13 14 2 15 11 -9 17 

 1529 36 19 32 3 20 9 20 17 -1 4 2 22 40 30 98 

 1530 51 22 24 18 5 22 8 8 13 18 -16 

 1531 35 36 17 24 17 17 -4 20 -2 31 3 

 1532 31 25 25 19 18 6 8 11 2 1 5 

 1533 39 26 17 19 14 13 5 6 3 6 1 

 1534 32 28 19 19 12 8 3 11 8 4 5 

 1535 35 16 30 21 5 7 18 5 -1 7 -5 

 1536 35 21 19 11 17 16 4 1 7 9 -2 

 1537 36 29 17 26 7 9 0 15 -1 5 1 

 1538 42 27 24 18 6 21 5 7 5 13 3 

 1539 37 24 27 14 17 18 12 -1 9 0 7 

 1540 40 22 30 22 11 6 17 9 6 -4 10 

 1541 35 21 26 17 -2 17 11 6 -3 13 -5 

 1542 42 29 27 20 5 10 14 19 -2 3 7 

 1543 37 31 26 18 10 11 9 10 10 2 11 

 1544 45 30 31 17 20 25 12 11 5 10 -10 

 1545 40 32 18 33 9 18 2 23 10 -4 18 

 1546 27 19 6 22 10 7 -2 16 4 4 5 

 1547 31 29 18 14 4 11 5 16 4 0 9 

 1548 33 35 22 19 12 9 16 10 1 7 -11 29 4 -17 -1 16 

 1549 38 23 30 15 2 21 0 18 1 8 1 

 1550 37 15 23 19 13 12 3 15 7 -4 12 4 -5 -1 12 5 

 1551 39 15 31 20 11 14 -7 22 -3 13 -9 

MEAN 37 25 24 19 11 13 7 11 5 5 2 18 13 4 36 11 

S.D. 5.3 5.8 6.1 5.8 6.2 5.3 6.8 6.4 5.4 8.1 8.5 13.0 23.3 23.9 53.5 8.1

 N 26 26 26 26 26 26 26 26 26 26 26 3 3 3 3 2
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Huntingdon Life Sciences 00-4206 PAGE 284 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 38 28 5 22 13 12 4 17 9 4 -1 

 2527 34 21 30 23 9 2 12 9 -3 0 6 -1 11 6 13 7 

 2528 29 31 19 23 5 16 5 10 -5 0 5 

 2529 35 33 25 17 26 17 9 -2 9 4 -6 

 2530 29 31 28 23 -2 27 6 16 9 -8 19 6 

 2531 30 35 20 10 19 14 13 -5 6 31 14 

 2532 34 23 18 19 13 15 9 4 8 22 -9 

 2533 33 27 22 24 13 3 17 13 4 0 11 

 2534 30 23 28 10 18 8 13 16 1 -3 7 

 2535 33 28 29 10 8 16 12 7 20 26 -15 

 2536 39 34 21 24 15 19 5 17 0 4 1 

 2537 33 27 13 24 13 10 6 6 8 -8 2 

 2538 47 17 33 19 6 15 4 13 20 -8 -10 2 28 -11 6 8 

 2539 33 28 23 17 8 17 9 11 3 4 2 

 2540 32 25 25 10 19 13 8 12 1 12 -8 

 2541 46 41 38 20 23 11 16 18 -11 12 5 

 2542 36 21 19 18 11 2 17 8 8 3 0 18 2 -3 12 9 

 2543 40 28 21 11 17 23 9 17 15 0 9 

 2544 50 13 15 28 10 24 5 11 -1 13 -2 

 2545 34 24 23 18 9 16 7 6 7 6 2 

 2546 42 10 28 16 13 1 18 3 6 -2 11 

 2547 26 24 18 15 8 12 10 3 1 10 1 

 2548 34 27 23 19 14 14 6 7 12 19 16 

 2549 33 28 15 27 11 15 20 -1 12 15 -9 1a

 

 2550 36 24 28 11 7 14 10 5 2 7 -3 

 2551 35 26 19 25 18 15 4 12 11 8 3 

MEAN 35 26 22 19 12 14 10 9 6 6 2 6 14 -3 10 6

S.D. 5.6 6.6 6.9 5.6 5.9 6.5 4.8 6.3 7.2 10.2 8.5 8.3 13.4 8.4 4.1 3.7 

 N 26 26 26 26 26 26 26 26 26 26 26 4 3 3 3 4

a

Animal was pregnant but had total litter loss. Recording of body weights was resumed.
23-JUN-2009 18:25

Huntingdon Life Sciences 00-4206 PAGE 285 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 38 31 15 23 14 0 16 8 8 -4 10 

 3527 38 29 17 22 18 12 -3 22 8 5 4 

 3528 28 18 23 8 7 14 2 18 9 15 -7 

 3529 32 24 21 7 18 9 7 0 9 7 -6 

 3530 39 27 10 30 12 11 11 16 -10 11 15 

 3531 31 23 11 26 13 10 3 11 4 5 1 

 3532 29 24 23 17 14 7 9 11 -2 14 7 

 3533 39 36 29 25 1 22 6 0 6 6 -4 

 3534 34 25 12 21 14 4 2 12 2 -2 10 

 3535 39 28 19 10 21 14 17 -9 11 5 25 

 3536 50 18 27 21 8 8 16 10 2 -3 13 3a 

 3537 28 17 28 16 12 -1 13 12 -3 27 12 

 3538 37 33 22 18 15 9 8 9 -2 24 7 -10 4 -12 15 5 

 3539 35 29 24 9 25 15 10 1 15 8 -5 29 26 -11 -8 -3 

 3540 32 27 17 21 18 11 10 10 6 3 4 

 3541 47 22 27 14 12 12 2 10 12 14 -12 

 3542 40 29 20 19 15 12 9 2 15 12 -4 

 3543 34 19 20 17 3 15 5 5 19 14 -7 

 3544 34 24 20 12 10 9 12 3 10 4 -5 

 3545 29 31 18 22 7 18 -2 17 11 14 -13 

 3546 27 33 13 28 9 1 14 0 9 14 24 

 3547 26 24 7 26 -2 18 13 8 -3 12 4 

 3548 39 35 30 13 25 9 9 -3 18 16 -20 12a

 

 3549 33 32 24 14 12 -3 21 6 -3 7 0 

 3550 36 40 20 19 8 9 16 4 6 19 16 9 17 -24 6 6 

 3551 37 40 12 27 1 19 2 18 12 -6 9 

MEAN 35 28 19 19 12 10 9 8 7 9 3 9 16 -16 4 5

S.D. 5.8 6.5 6.3 6.4 6.9 6.3 6.2 7.4 7.2 8.1 11.1 19.5 10.9 7.1 11.4 5.1 

 N 26 26 26 26 26 26 26 26 26 26 26 3 3 3 3 5

a

Animal was pregnant but had total litter loss. Recording of body weights was resumed.
23-JUN-2009 18:25

Huntingdon Life Sciences 00-4206 PAGE 286 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 27 22 20 25 15 16 5 12 8 -2 -4 

 4527 32 33 24 23 18 2 17 12 12 -11 11 

 4528 34 21 28 21 10 22 13 7 -2 10 -5 

 4529 44 9 23 17 16 8 14 9 -3 9 -7 

 4530 35 13 18 2 27 6 -1 18 9 -1 2 

 4531 32 11 26 19 8 7 25 -6 3 -1 1 

 4532 28 15 10 27 4 5 21 8 -2 2 13 

 4533 29 15 6 20 15 15 11 5 0 12 1 

 4534 35 20 23 15 10 19 11 5 -8 14 -3 

 4535 36 14 18 12 12 9 5 8 1 8 2 

 4536 29 10 34 16 13 6 19 0 5 -7 16 

 4537 33 12 28 5 28 4 15 2 8 7 1 

 4538 32 27 20 13 17 10 14 -5 10 11 -7 

 4539 29 25 12 21 4 19 8 2 10 13 -16 

 4540 28 15 23 19 2 19 7 12 3 -4 9 

 4541 37 33 20 4 22 16 10 -7 9 11 -1 6 4 0 3 14 

 4542 42 25 27 4 25 6 11 10 -8 17 4 

 4543 45 22 15 24 17 -7 28 -5 14 4 11 

 4544 36 15 28 15 9 5 12 5 1 1 8 

 4545 34 22 20 13 13 6 2 16 9 -4 2 

 4546 39 13 33 18 9 0 24 8 3 -5 17 

 4547 41 14 29 19 17 34 -10 -10 15 11 -9 30 21 49 

 4548 43 26 13 27 15 10 4 14 5 2 15 

 4549 24 28 12 15 -4 19 

 4550 27 32 18 25 11 10 8 7 6 3 9 13 

 4551 36 27 16 14 22 13 6 2 9 0 -1 

MEAN 34 20 21 17 14 11 11 5 5 4 3 16 13 25 3 14

S.D. 5.7 7.4 7.2 7.1 7.7 8.3 8.5 7.4 6.2 7.4 8.5 12.1 12.4 34.6 0.0 0.0 

 N 26 26 26 26 26 26 25 25 25 25 25 3 2 2 1 1 
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Huntingdon Life Sciences 00-4206 PAGE 287 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 117 

 1527 136 

 1528 136 

 1529 125 

 1530 122 

 1531 160 

 1532 119 

 1533 110 

 1534 118 

 1535 103 

 1536 102 

 1537 108 

 1538 129 

 1539 128 

 1540 129 

 1541 103 

 1542 131 

 1543 137 

 1544 151 

 1545 159 

 1546 91 

 1547 110 

 1548 120 

 1549 119 

 1550 115 

 1551 107 

MEAN 122

S.D. 17.2

 N 26
23-JUN-2009 18:25

Huntingdon Life Sciences 00-4206 PAGE 288 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 112 

 2527 108 

 2528 110 

 2529 130 

 2530 149 

 2531 157 

 2532 121 

 2533 134 

 2534 121 

 2535 141 

 2536 139 

 2537 100 

 2538 108 

 2539 122 

 2540 117 

 2541 173 

 2542 106 

 2543 149 

 2544 115 

 2545 118 

 2546 103 

 2547 102 

 2548 158 

 2549 133 

 2550 104 

 2551 142 

MEAN 126

S.D. 20.1

 N 26
23-JUN-2009 18:25

Huntingdon Life Sciences 00-4206 PAGE 289 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 121 

 3527 133 

 3528 107 

 3529 94 

 3530 132 

 3531 108 

 3532 123 

 3533 128 

 3534 101 

 3535 140 

 3536 121 

 3537 133 

 3538 144 

 3539 131 

 3540 127 

 3541 113 

 3542 126 

 3543 110 

 3544 99 

 3545 123 

 3546 146 

 3547 106 

 3548 131 

 3549 110 

 3550 157 

 3551 133 

MEAN 123

S.D. 15.6

 N 26
23-JUN-2009 18:25

Huntingdon Life Sciences 00-4206 PAGE 290 

 

 APPENDIX H 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 116 

 4527 141 

 4528 126 

 4529 96 

 4530 94 

 4531 94 

 4532 103 

 4533 100 

 4534 105 

 4535 88 

 4536 113 

 4537 109 

 4538 110 

 4539 96 

 4540 104 

 4541 117 

 4542 123 

 4543 122 

 4544 98 

 4545 98 

 4546 121 

 4547 110 

 4548 131 

 4550 128 

 4551 107 

MEAN 110

S.D. 13.6

 N 25
23-JUN-2009 18:28

Huntingdon Life Sciences 00-4206 PAGE 291 

 

 APPENDIX I 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 1526 270 297 305 338 401 

 1527 DPP 320 343 362 385 453 

 1528 304 334 347 373 442 

 1529xPNMD 

 1530 305 329 340 370 423 

 1531 330 347 356 388 457 

 1532x NP 274 298 300 321 317 

 1533 272 299 305 331 362 

 1534 278 296 304 340 403 

 1535x NP 259 285 300 326 299 

 1536 282 307 315 344 392 

 1537 265 284 284 320 379 

 1538 297 314 327 351 424 

 1539 287 313 316 352 402 

 1540 298 319 327 356 430 

 1541x NP 263 283 289 297 283 

 1542 325 344 350 382 445 

 1543 305 322 335 362 439 

 1544 335 375 393 427 460 

 1545 341 365 379 410 485 

 1546 251 275 288 319 379 

 1547 258 278 291 313 379 

 1548xNNMD 

 1549 282 312 322 345 404 

 1550xNNMD 

 1551 272 291 293 324 398 

MEAN 294 317 327 357 418

S.D. 26.4 27.9 30.6 30.8 33.0

 N 20 20 20 20 20

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 

 x=EXCLUDED FROM MEAN 
23-JUN-2009 18:28

Huntingdon Life Sciences 00-4206 PAGE 292 

 

 APPENDIX I 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 2526 265 291 303 330 394 

 2527xNNMD 

 2528 288 310 320 340 419 

 2529 303 325 336 366 422 

 2530 327 372 383 417 473 

 2531 290 317 328 352 436 

 2532x NP 275 280 286 287 289 

 2533 293 316 325 351 421 

 2534 268 298 309 333 380 

 2535 306 330 340 369 433 

 2536 298 331 338 375 447 

 2537 252 283 297 333 385 

 2538xNNMD 

 2539 280 300 312 349 404 

 2540 271 293 307 330 381 

 2541 356 387 401 432 481 

 2542xNNMD 

 2543 326 344 352 371 442 

 2544 301 333 344 367 405 

 2545 288 313 325 348 392 

 2546 255 275 292 315 384 

 2547 243 263 268 296 357 

 2548 307 324 329 357 378 

 2549 TLL 291 326 335 352 369 

 2550 282 305 312 333 382 

 2551 309 340 353 386 435 

MEAN 291 317 328 355 410

S.D. 26.5 29.2 29.2 30.9 33.6

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:28

Huntingdon Life Sciences 00-4206 PAGE 293 

 

 APPENDIX I 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 3526x NP 299 297 301 305 307 

 3527 301 346 353 388 470 

 3528 267 290 293 328 378 

 3529 260 288 298 334 406 

 3530 280 316 325 352 409 

 3531 258 288 301 343 422 

 3532 295 321 329 356 427 

 3533 302 327 337 366 433 

 3534 264 294 310 335 409 

 3535 305 321 329 353 399 

 3536 TLL 291 313 324 347 326 

 3537 270 298 303 332 402 

 3538xNNMD 

 3539xNNMD 

 3540 274 305 314 349 368 

 3541 280 302 316 336 393 

 3542 318 342 347 384 457 

 3543 270 292 299 303 391 

 3544 259 282 290 314 368 

 3545 276 293 301 332 400 

 3546 294 317 324 351 426 

 3547 259 296 303 335 405 

 3548 TLL 319 347 362 381 443 

 3549 273 299 312 349 424 

 3550xNNMD 

 3551 303 327 344 369 407 

MEAN 283 309 319 347 407

S.D. 19.5 19.7 20.4 21.3 31.5

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:28

Huntingdon Life Sciences 00-4206 PAGE 294 

 

 APPENDIX I 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 4526 284 301 309 340 404 

 4527 298 336 340 374 438 

 4528 292 313 318 352 422 

 4529 279 305 317 351 404 

 4530x NP 256 277 295 306 272 

 4531 254 267 274 300 356 

 4532 259 289 296 332 405 

 4533 278 307 324 359 434 

 4534 300 319 332 365 435 

 4535 243 257 267 293 346 

 4536 263 285 295 335 358 

 4537 286 317 330 349 403 

 4538 273 288 301 329 408 

 4539x NP 249 264 281 290 262 

 4540 253 285 297 319 368 

 4541xNNMD 

 4542 273 302 306 337 399 

 4543 286 313 318 357 410 

 4544 262 282 296 326 402 

 4545 278 302 310 342 390 

 4546 279 319 325 355 430 

 4547xPNMD 

 4548 289 316 328 357 432 

 4549 DIED 

 4550 290 322 332 368 436 

 4551 261 287 301 323 381 

MEAN 275 301 310 341 403

S.D. 15.8 19.5 19.1 21.2 28.0

 N 21 21 21 21 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN 
23-JUN-2009 18:30

Huntingdon Life Sciences 00-4206 PAGE 295 

 

 APPENDIX J 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 1526 27 8 33 63 131 

 1527 DPP 23 19 24 68 134 

 1528 30 12 26 70 138 

 1529xPNMD 

 1530 24 11 30 53 119 

 1531 17 9 32 69 127 

 1532x NP 24 2 21 -4 44 

 1533 27 6 26 31 90 

 1534 17 8 36 63 125 

 1535x NP 26 14 27 -27 40 

 1536 24 9 29 48 110 

 1537 19 1 36 59 114 

 1538 17 13 24 73 127 

 1539 26 3 37 50 116 

 1540 21 8 29 75 132 

 1541x NP 20 6 8 -14 21 

 1542 20 5 33 62 120 

 1543 16 14 27 77 134 

 1544 41 18 34 33 125 

 1545 24 15 31 74 144 

 1546 24 13 32 60 128 

 1547 20 13 21 66 120 

 1548xNNMD 

 1549 30 10 23 59 122 

 1550xNNMD 

 1551 19 1 31 74 126 

MEAN 23 10 30 61 124

S.D. 5.9 5.0 4.6 12.9 11.5

 N 20 20 20 20 20

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 

 x=EXCLUDED FROM MEAN 
23-JUN-2009 18:30

Huntingdon Life Sciences 00-4206 PAGE 296 

 

 APPENDIX J 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 2526 25 12 28 64 128 

 2527xNNMD 

 2528 22 10 19 80 131 

 2529 23 11 30 56 119 

 2530 44 12 34 56 146 

 2531 28 10 25 83 146 

 2532x NP 5 7 0 2 14 

 2533 22 9 26 70 128 

 2534 30 12 24 47 112 

 2535 25 10 29 63 127 

 2536 33 7 36 73 150 

 2537 31 15 36 52 133 

 2538xNNMD 

 2539 20 12 36 56 124 

 2540 22 14 23 50 109 

 2541 31 14 31 50 125 

 2542xNNMD 

 2543 18 8 19 71 116 

 2544 32 11 23 38 104 

 2545 24 12 22 44 103 

 2546 21 17 22 70 130 

 2547 19 5 28 62 114 

 2548 17 5 28 21 71 

 2549 TLL 35 9 17 17 79 

 2550 23 7 21 49 100 

 2551 31 13 34 49 126 

MEAN 26 11 27 55 119

S.D. 6.6 3.1 5.9 16.6 19.7

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:30

Huntingdon Life Sciences 00-4206 PAGE 297 

 

 APPENDIX J 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 3526x NP -2 4 4 1 8 

 3527 44 8 34 83 169 

 3528 23 4 35 50 112 

 3529 28 10 36 72 147 

 3530 36 9 27 57 129 

 3531 30 13 41 80 164 

 3532 26 9 27 71 132 

 3533 26 10 28 67 131 

 3534 30 16 25 75 145 

 3535 16 8 25 45 94 

 3536 TLL 22 10 23 -21 35 

 3537 28 5 29 71 133 

 3538xNNMD 

 3539xNNMD 

 3540 31 9 36 18 94 

 3541 22 14 20 57 113 

 3542 24 5 37 73 139 

 3543 22 8 4 88 122 

 3544 23 8 25 53 108 

 3545 17 8 32 68 124 

 3546 23 7 27 75 132 

 3547 37 7 32 70 147 

 3548 TLL 28 15 18 63 124 

 3549 26 14 36 75 150 

 3550xNNMD 

 3551 25 16 25 38 104 

MEAN 27 10 28 60 125

S.D. 6.5 3.6 8.1 24.1 28.4

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:30

Huntingdon Life Sciences 00-4206 PAGE 298 

 

 APPENDIX J 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 4526 17 8 31 64 120 

 4527 38 4 35 64 139 

 4528 21 4 34 71 130 

 4529 26 13 33 53 125 

 4530x NP 21 18 11 -34 16 

 4531 13 7 26 56 102 

 4532 30 7 36 73 146 

 4533 30 17 35 75 157 

 4534 18 14 33 70 134 

 4535 14 10 26 53 103 

 4536 22 10 39 24 95 

 4537 31 13 19 54 117 

 4538 16 12 28 79 136 

 4539x NP 16 17 9 -28 13 

 4540 32 12 22 50 115 

 4541xNNMD 

 4542 30 4 31 61 126 

 4543 28 5 39 53 124 

 4544 20 14 30 76 140 

 4545 23 8 32 48 112 

 4546 40 6 30 75 151 

 4547xPNMD 

 4548 27 12 29 75 143 

 4549 DIED 

 4550 32 10 36 67 146 

 4551 26 14 22 57 120 

MEAN 25 10 31 62 128

S.D. 7.4 3.8 5.5 13.2 16.9

 N 21 21 21 21 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN 
23-JUN-2009 18:32

Huntingdon Life Sciences 00-4206 PAGE 299 

 

 APPENDIX K 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 1526 296 318 290 336 318 319 

 1527 DPP 

 1528 339 357 371 387 382 350 

 1529 PNMD a 363 375 391 398 358 

 1530 326 332 359 369 365 341 

 1531 366 362 376 399 369 347 

 1532x NP 

 1533 301 315 332 356 335 340 

 1534 317 319 345 361 353 317 

 1535x NP 

 1536 308 327 337 357 373 322 

 1537 286 303 326 350 326 302 

 1538 319 312 343 362 348 320 

 1539 331 337 360 371 354 330 

 1540 326 335 352 361 350 320 

 1541x NP 

 1542 341 342 368 382 365 347 

 1543 331 341 352 372 358 320 

 1544 361 387 422 427 399 360 

 1545 384 387 403 416 391 381 

 1546 288 279 313 341 309 299 

 1547 305 287 319 345 326 309 

 1548xNNMD 

 1549 315 311 344 371 356 332 

 1550xNNMD 

 1551 308 328 318 356 327 286 

MEAN 324 332 350 370 355 330

S.D. 26.3 29.1 31.1 23.9 26.2 23.4

 N 19 20 20 20 20 20

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 

 x=EXCLUDED FROM MEAN 

a

Body weight was not taken on Lactation Day 1 due to technician error. 
23-JUN-2009 18:32

Huntingdon Life Sciences 00-4206 PAGE 300 

 

 APPENDIX K 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 2526 291 295 300 345 340 320 

 2527xNNMD 

 2528 301 308 324 348 352 315 

 2529 318 324 344 353 356 348 

 2530 380 398 419 434 425 371 

 2531 299 319 328 342 336 310 

 2532x NP 

 2533 324 312 287 361 354 334 

 2534 279 279 307 313 320 295 

 2535 325 340 355 359 355 349 

 2536 338 358 352 367 360 338 

 2537 312 328 318 350 339 308 

 2538xNNMD 

 2539 316 323 332 354 338 344 

 2540 298 307 319 331 342 316 

 2541 388 373 342 398 386 392 

 2542xNNMD 

 2543 334 345 380 373 349 343 

 2544 330 341 349 355 344 337 

 2545 311 326 343 337 335 319 

 2546 291 296 292 338 321 292 

 2547 261 289 300 317 324 286 

 2548 322 337 343 358 351 342 

 2549 TLL 335 

 2550 322 321 337 349 355 325 

 2551 336 345 346 368 379 333 

MEAN 319 327 334 355 351 329

S.D. 29.0 28.3 30.3 26.0 23.7 25.6

 N 22 21 21 21 21 21

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:32

Huntingdon Life Sciences 00-4206 PAGE 301 

 

 APPENDIX K 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 3526x NP 

 3527 347 345 376 392 365 355 

 3528 294 314 317 335 338 307 

 3529 302 315 331 355 345 305 

 3530 319 331 354 363 323 325 

 3531 317 320 342 366 342 318 

 3532 338 355 376 401 381 362 

 3533 321 358 343 389 380 357 

 3534 321 314 332 374 343 313 

 3535 325 330 341 358 355 345 

 3536 TLL 

 3537 281 286 319 339 333 312 

 3538xNNMD 

 3539xNNMD 

 3540 318 299 324 335 342 321 

 3541 324 325 337 360 352 326 

 3542 352 333 384 403 362 355 

 3543 303 304 332 363 347 310 

 3544 280 292 315 327 341 304 

 3545 305 312 316 359 341 326 

 3546 312 343 355 374 359 345 

 3547 292 294 317 356 324 324 

 3548 TLL 353 

 3549 319 340 351 376 363 329 

 3550xNNMD 

 3551 348 350 358 373 366 337 

MEAN 318 323 341 365 350 329

S.D. 21.7 21.6 21.2 21.3 16.1 18.6

 N 21 20 20 20 20 20

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:32

Huntingdon Life Sciences 00-4206 PAGE 302 

 

 APPENDIX K 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 4526 320 315 333 341 336 292 

 4527 330 340 366 390 377 348 

 4528 315 328 323 340 333 336 

 4529 317 341 328 353 352 324 

 4530x NP 

 4531 267 292 306 330 316 318 

 4532 298 317 311 324 339 321 

 4533 322 337 358 388 351 359 

 4534 312 355 355 372 359 330 

 4535 271 273 292 302 300 277 

 4536 291 293 315 344 324 321 

 4537 330 352 366 373 352 361 

 4538 293 302 329 317 312 291 

 4539x NP 

 4540 293 290 306 333 300 304 

 4541xNNMD 

 4542 310 332 332 343 331 314 

 4543 318 321 312 376 351 350 

 4544 299 321 333 342 334 303 

 4545 298 322 341 328 365 322 

 4546 308 325 355 377 363 333 

 4547 PNMD 295 320 335 347 361 325 

 4548 323 336 348 364 360 349 

 4549 DIED 

 4550 339 360 368 380 381 359 

 4551 296 287 319 337 322 317 

MEAN 307 321 333 350 342 325

S.D. 18.2 23.4 21.7 24.3 23.2 23.2

 N 22 22 22 22 22 22

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN 
23-JUN-2009 18:34

Huntingdon Life Sciences 00-4206 PAGE 303 

 

 APPENDIX L 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 1526 21 -27 45 -18 1 23 

 1527 DPP 

 1528 19 14 15 -5 -32 12 

 1529 PNMD a 12 16 7 -40 

 1530 6 27 11 -4 -24 15 

 1531 -3 14 23 -30 -22 -19 

 1532x NP 

 1533 14 18 24 -22 5 39 

 1534 2 27 16 -8 -36 1 

 1535x NP 

 1536 19 10 20 16 -51 13 

 1537 17 23 24 -24 -24 16 

 1538 -6 31 19 -14 -28 1 

 1539 6 23 12 -17 -24 -2 

 1540 10 17 9 -12 -30 -6 

 1541x NP 

 1542 1 26 14 -17 -18 6 

 1543 11 11 20 -15 -38 -11 

 1544 26 35 5 -28 -39 -1 

 1545 3 16 13 -25 -10 -2 

 1546 -9 34 29 -32 -10 11 

 1547 -19 33 26 -20 -17 4 

 1548xNNMD 

 1549 -5 33 27 -15 -24 17 

 1550xNNMD 

 1551 20 -10 37 -28 -42 -22 

MEAN 7 18 20 -15 -25 5

S.D. 11.9 15.3 9.7 12.3 14.3 14.6

 N 19 20 20 20 20 19

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 

 x=EXCLUDED FROM MEAN 

a

Body weight was not taken on Lactation Day 1 due to technician error. 
23-JUN-2009 18:34

Huntingdon Life Sciences 00-4206 PAGE 304 

 

 APPENDIX L 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 2526 4 5 45 -5 -20 29 

 2527xNNMD 

 2528 6 16 25 4 -37 13 

 2529 7 20 9 4 -8 30 

 2530 18 22 15 -9 -54 -8 

 2531 20 9 14 -6 -26 12 

 2532x NP 

 2533 -12 -25 74 -7 -20 10 

 2534 -1 29 6 7 -25 16 

 2535 15 14 5 -4 -7 24 

 2536 20 -6 15 -7 -22 0 

 2537 16 -10 32 -11 -31 -4 

 2538xNNMD 

 2539 6 9 23 -17 7 28 

 2540 10 12 11 11 -26 18 

 2541 -16 -31 56 -12 6 4 

 2542xNNMD 

 2543 11 36 -7 -25 -5 9 

 2544 12 8 6 -11 -7 7 

 2545 14 17 -6 -2 -17 7 

 2546 5 -4 46 -17 -30 0 

 2547 28 11 17 6 -38 25 

 2548 14 6 15 -7 -9 20 

 2549 TLL 

 2550 -1 16 12 6 -30 3 

 2551 9 2 21 11 -46 -3 

MEAN 9 7 21 -4 -21 11

S.D. 10.4 15.9 20.0 9.7 15.8 11.7

 N 21 21 21 21 21 21

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:34

Huntingdon Life Sciences 00-4206 PAGE 305 

 

 APPENDIX L 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28 

-----------------------------------------------------------------------------------------------------------------------------------

 3526x NP 

 3527 -1 31 16 -27 -10 8 

 3528 21 2 18 3 -31 14 

 3529 12 16 25 -10 -40 3 

 3530 12 23 9 -40 2 6 

 3531 3 22 24 -24 -24 1 

 3532 17 21 25 -20 -19 23 

 3533 37 -15 46 -10 -22 36 

 3534 -7 19 41 -30 -30 -8 

 3535 4 11 17 -3 -10 19 

 3536 TLL 

 3537 5 33 19 -6 -21 32 

 3538xNNMD 

 3539xNNMD 

 3540 -18 25 10 7 -21 4 

 3541 1 11 23 -8 -26 2 

 3542 -19 51 19 -41 -7 4 

 3543 1 28 32 -17 -37 8 

 3544 12 23 12 14 -37 24 

 3545 7 4 44 -18 -15 21 

 3546 31 12 19 -14 -15 33 

 3547 2 24 39 -33 1 32 

 3548 TLL 

 3549 21 11 25 -13 -34 10 

 3550xNNMD 

 3551 2 8 16 -7 -29 -11 

MEAN 7 18 24 -15 -21 13

S.D. 14.1 13.7 11.1 14.8 12.2 13.8

 N 20 20 20 20 20 20

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:34

Huntingdon Life Sciences 00-4206 PAGE 306 

 

 APPENDIX L 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 4526 -5 18 8 -5 -44 -28 

 4527 10 26 24 -13 -30 18 

 4528 13 -5 17 -7 3 21 

 4529 25 -13 25 -1 -28 7 

 4530x NP 

 4531 24 15 23 -14 3 51 

 4532 19 -6 13 15 -19 23 

 4533 15 21 30 -36 7 37 

 4534 43 1 17 -13 -29 18 

 4535 2 19 10 -2 -23 6 

 4536 2 22 29 -20 -3 30 

 4537 22 13 7 -21 9 31 

 4538 9 27 -13 -4 -21 -3 

 4539x NP 

 4540 -3 15 27 -33 4 11 

 4541xNNMD 

 4542 22 0 11 -12 -17 5 

 4543 3 -9 64 -25 -1 32 

 4544 22 12 9 -8 -31 4 

 4545 25 19 -13 37 -43 24 

 4546 17 29 23 -15 -30 25 

 4547 PNMD 25 14 13 13 -35 30 

 4548 14 12 16 -4 -11 26 

 4549 DIED 

 4550 21 8 13 1 -22 21 

 4551 -9 32 18 -15 -5 21 

MEAN 14 12 17 -8 -17 19

S.D. 12.4 12.9 15.4 16.0 16.5 16.2

 N 22 22 22 22 22 22

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN 
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 307 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 135 109 96 89 80 71 71 66 62 61 60 53 50 50 50 

 1027 150 117 101 87 76 67 59 62 57 56 56 51 47 44 47 

 1028 149 121 92 94 80 71 70 68 62 61 58 55 51 50 48 

 1029 144 112 100 89 73 69 65 63 59 58 56 49 45 43 45 

 1030 151 117 102 92 76 75 69 65 59 59 54 48 46 44 46 

 1031 145 114 101 90 74 66 64 60 60 56 56 45 44 48 47 

 1032 148 114 103 86 81 76 67 64 61 61 59 48 45 46 46 

 1033 153 116 104 93 78 71 65 62 59 57 54 49 48 47 49 

 1034 148 119 104 91 78 72 63 60 59 60 53 49 49 44 43 

 1035 144 106 95 85 75 74 66 57 61 62 58 52 54 52 50 

 1036 152 113 101 94 79 75 63 61 62 58 50 48 47 44 45 

 1037 162 122 109 98 89 79 73 64 64 60 55 51 53 49 50 

 1038 160 127 105 94 81 72 68 65 59 55 49 46 47 43 44 

 1039 157 120 104 89 81 77 70 65 62 63 59 54 50 46 46 

 1040 165 121 106 94 83 74 68 66 61 57 54 51 53 49 47 

 1041 154 116 100 89 80 73 64 62 63 60 58 54 51 48 47 

 1042 153 115 101 93 73 70 67 63 55 55 52 51 48 48 45 

 1043 142 115 100 88 75 74 67 65 61 61 57 52 51 49 48 

 1044 155 118 106 95 79 77 71 65 63 61 57 52 51 50 48 

 1045 163 118 106 100 85 84 77 75 74 74 68 62 59 62 57 

 1046 162 121 104 92 79 75 67 64 62 62 58 50 49 46 46 

 1047 153 120 107 93 79 72 67 60 63 63 57 53 47 46 48 

 1048 168 121 108 97 79 74 65 65 66 60 52 50 54 52 47 

 1049 164 126 114 103 87 82 78 65 64 65 63 58 56 53 51 

 1050 162 126 109 95 82 77 73 65 62 62 56 51 50 47 44 

 1051 154 112 98 86 74 71 66 60 59 57 53 47 46 43 45 

MEAN 153 117 103 92 79 74 68 64 62 60 56 51 50 48 47

S.D. 8.1 5.1 4.8 4.4 4.1 4.1 4.3 3.4 3.5 3.8 3.9 3.6 3.6 4.2 2.9

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 308 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 141 109 94 83 79 72 66 60 59 59 53 52 51 48 49 

 2027 145 111 98 92 78 72 68 62 58 58 54 49 48 49 46 

 2028 154 111 94 89 78 68 63 64 58 51 54 48 47 47 46 

 2029 151 118 106 95 80 74 69 64 63 64 56 51 48 47 47 

 2030 147 111 99 88 77 71 67 62 57 55 53 48 48 46 42 

 2031 141 110 96 85 74 67 63 58 57 56 54 46 46 47 44 

 2032 159 114 100 87 77 69 68 61 59 58 56 51 49 47 42 

 2033 151 117 105 88 76 69 65 58 59 57 54 48 47 49 46 

 2034 143 115 99 87 76 74 68 62 63 61 55 51 50 51 48 

 2035 163 117 102 89 82 72 66 63 58 58 54 49 47 51 51 

 2036 148 107 95 81 71 67 63 60 59 59 55 46 46 49 46 

 2037 156 117 100 90 72 70 65 61 55 58 51 47 44 46 43 

 2038 151 114 96 83 78 70 65 64 61 59 52 47 46 45 43 

 2039 155 117 102 91 82 74 69 66 65 61 57 51 48 47 45 

 2040 164 125 106 99 85 77 68 69 66 63 61 56 54 49 48 

 2041 144 108 85 85 73 68 60 58 58 58 47 46 49 44 40 

 2042 159 120 100 92 78 69 62 60 55 55 52 47 46 44 40 

 2043 166 119 103 91 75 71 66 66 60 58 55 49 49 49 47 

 2044 166 122 103 90 74 72 67 66 62 62 60 54 52 52 47 

 2045 151 114 94 86 77 71 64 62 59 57 51 49 49 49 45 

 2046 171 122 106 93 84 75 70 64 60 61 59 54 54 54 52 

 2047 164 117 98 87 75 72 68 62 60 59 55 48 46 46 47 

 2048 156 110 105 93 81 76 71 64 58 61 56 48 49 49 46 

 2049 154 113 93 85 78 71 66 65 59 58 57 50 50 48 47 

 2050 157 126 107 89 84 75 69 67 59 57 55 48 48 44 44 

 2051 169 120 104 88 81 72 67 63 61 59 57 52 47 45 43 

MEAN 155 115 100 89 78 72 66 63 60 58 55 49 48 48 45

S.D. 8.7 5.1 5.3 4.0 3.7 2.7 2.7 2.7 2.6 2.7 3.0 2.6 2.4 2.6 3.0

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 309 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 152 118 104 89 81 71 65 65 62 61 55 52 51 50 48 

 3027 143 113 96 82 72 67 63 57 55 56 51 46 44 46 47 

 3028 132 114 98 83 73 67 62 58 58 58 53 46 44 45 45 

 3029 148 116 97 88 76 70 64 59 59 57 50 48 46 46 46 

 3030 148 116 99 90 79 72 66 59 55 55 50 49 46 45 46 

 3031 157 123 101 90 79 75 70 65 62 61 55 53 49 52 52 

 3032 153 116 100 90 76 71 67 63 61 59 55 54 51 51 50 

 3033 149 118 97 91 82 74 68 63 60 58 54 51 48 49 49 

 3034 158 118 100 92 79 71 67 61 60 62 56 49 48 49 49 

 3035 137 105 95 81 73 70 64 58 59 59 54 51 48 49 52 

 3036 149 115 99 89 80 70 65 63 59 60 54 48 47 46 45 

 3037 145 114 100 86 82 73 65 64 61 62 54 51 48 52 52 

 3038 148 109 94 84 74 66 63 63 59 58 54 48 46 44 44 

 3039 158 126 100 88 91 82 73 69 63 62 53 51 49 48 43 

 3040 148 114 99 88 81 71 64 62 60 59 53 51 48 45 44 

 3041 149 118 95 84 72 70 64 59 59 63 54 50 46 46 47 

 3042 146 114 99 89 85 73 67 65 61 59 52 50 49 48 46 

 3043 150 119 101 87 73 69 65 66 62 60 55 52 50 48 48 

 3044 155 117 100 87 72 70 66 63 59 61 56 51 48 45 48 

 3045 144 117 97 90 78 70 65 63 58 60 56 54 52 47 50 

 3046 154 118 96 93 82 77 69 62 64 61 53 53 51 48 48 

 3047 141 113 96 86 72 67 66 63 60 60 54 48 45 44 45 

 3048 158 117 105 96 83 72 70 68 64 64 60 53 51 50 47 

 3049 143 110 96 86 79 68 67 64 60 60 57 55 51 50 48 

 3050 153 119 107 93 81 75 70 65 62 61 56 51 48 48 48 

 3051 161 125 108 97 85 74 67 66 59 60 55 49 46 45 41 

MEAN 149 116 99 88 79 71 66 63 60 60 54 51 48 47 47

S.D. 6.9 4.5 3.6 3.9 5.0 3.6 2.5 3.0 2.3 2.0 2.2 2.3 2.2 2.3 2.7

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 310 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 140 110 91 81 73 66 58 56 58 57 55 47 47 48 48 

 4027 129 97 90 82 74 68 63 62 62 60 57 50 47 52 54 

 4028 162 127 106 95 88 80 76 70 69 65 63 58 57 55 53 

 4029 151 112 96 87 79 72 66 62 59 58 54 53 50 45 44 

 4030 141 109 97 89 77 71 65 61 59 57 51 48 45 46 46 

 4031 150 116 98 90 81 70 68 63 60 60 55 51 48 50 50 

 4032 150 122 102 92 77 70 65 62 59 59 53 49 52 48 48 

 4033 150 114 94 88 77 68 65 60 60 60 56 51 42 45 49 

 4034 150 113 96 84 75 71 63 57 59 59 51 52 52 48 49 

 4035 141 112 103 93 79 73 68 63 58 55 51 50 49 47 43 

 4052 IW 116 101 92 82 76 72 67 58 63 57 55 49 52 49 

 4037 141 108 89 81 73 66 64 61 59 56 54 47 45 52 51 

 4038 144 112 95 88 77 70 68 65 64 61 54 50 54 52 53 

 4039 156 121 104 93 79 72 67 64 59 57 54 52 53 50 48 

 4040 158 114 99 96 85 78 72 66 64 61 57 52 56 52 52 

 4041 144 112 98 90 81 74 63 59 60 58 51 50 49 46 45 

 4042 139 107 95 90 80 74 70 66 64 64 60 56 51 50 53 

 4043 147 114 101 97 86 84 70 65 67 65 58 58 55 56 53 

 4044 152 111 99 91 81 76 68 64 63 61 57 51 47 48 47 

 4045 154 116 99 93 82 76 69 64 61 60 7 52 51 50 48 

 4046 162 120 103 99 77 72 69 62 63 59 54 47 48 47 47 

 4047 150 120 108 103 90 82 76 69 66 64 57 57 58 56 56 

 4048 152 119 99 95 85 71 67 63 60 58 53 53 51 53 51 

 4049 147 115 98 88 79 74 67 64 65 62 53 57 56 55 54 

 4050 150 117 101 93 80 78 65 62 63 63 62 57 52 52 52 

 4051 150 114 99 89 80 75 70 63 61 60 53 52 51 48 46 

MEAN 148 114 98 91 80 73 68 63 62 60 53 52 51 50 50

S.D. 7.5 5.7 4.6 5.2 4.3 4.5 4.1 3.2 2.9 2.8 10.0 3.3 4.0 3.3 3.4

 N 25 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 IW=Invalid Weight: Feed left greater than feed fed weight 
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 311 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 126 98 91 85 80 76 68 68 67 59 55 

 1527 125 94 95 87 81 78 69 72 70 64 62 

 1528 134 95 93 89 78 74 70 71 68 63 61 

 1529 133 96 96 83 78 76 71 74 69 65 67 72 58 

 1530 131 93 92 86 81 82 74 76 70 73 62 

 1531 121 99 92 91 81 75 66 70 65 72 70 

 1532 119 93 83 80 75 70 66 67 63 63 62 

 1533 133 103 94 93 86 81 72 73 72 69 62 

 1534 SF 102 94 91 83 74 74 74 72 69 SF 

 1535 120 94 91 86 72 76 74 72 65 70 62 

 1536 125 98 91 92 87 83 77 76 73 69 65 

 1537 133 108 98 97 90 85 78 78 67 73 65 

 1538 116 91 88 84 74 74 65 66 65 64 61 

 1539 122 95 92 82 81 78 72 67 65 61 63 

 1540 139 100 98 90 80 78 77 70 66 64 64 

 1541 128 96 100 90 81 83 74 72 71 73 61 

 1542 127 103 96 88 83 76 73 69 65 62 63 

 1543 143 110 102 89 82 76 71 70 70 68 68 

 1544 130 101 86 89 87 78 68 68 65 59 60 

 1545 111 86 93 76 74 70 65 66 62 63 62 

 1546 109 89 85 85 75 69 70 72 63 63 64 

 1547 134 108 97 100 85 88 76 77 71 71 68 

 1548 119 100 94 87 84 79 75 71 70 69 59 49 55 59 

 1549 130 108 99 89 84 87 77 76 65 68 63 

 1550 135 96 96 87 77 73 65 70 65 63 67 57 58 55 

 1551 130 94 98 86 75 74 63 70 63 63 56 

MEAN 127 98 94 88 80 78 71 71 67 66 63 59 57 57 

S.D. 8.3 6.1 4.5 4.9 4.6 5.0 4.4 3.5 3.3 4.4 3.6 11.2 2.0 2.6 

 N 25 26 26 26 26 26 26 26 26 26 25 3 3 2 

 SF=Spilled Feeder 
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 312 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 133 104 93 92 81 79 76 78 71 68 67 

 2527 123 100 98 89 83 79 77 74 70 70 68 63 62 60 

 2528 121 97 90 88 76 76 72 68 64 66 62 

 2529 118 94 85 89 83 74 65 61 64 57 52 

 2530 124 98 91 83 80 79 74 70 70 62 69 

 2531 129 109 99 93 91 82 76 73 71 79 80 

 2532 128 97 90 91 84 79 78 70 69 78 64 

 2533 128 97 91 92 82 78 74 71 69 67 66 

 2534 129 100 98 92 91 85 85 79 69 70 67 

 2535 127 109 100 91 85 83 73 73 73 86 64 

 2536 140 110 100 90 88 83 79 76 66 71 62 

 2537 129 102 95 96 89 87 82 75 74 70 66 

 2538 132 94 90 82 76 74 69 65 70 61 53 59 49 46 

 2539 135 106 104 95 85 85 85 77 73 72 77 

 2540 136 105 110 98 96 90 76 85 71 85 SF 

 2541 133 101 95 90 86 78 70 68 62 62 59 

 2542 128 96 90 80 83 80 73 65 60 61 61 56 56 54 

 2543 127 104 105 93 87 86 83 75 68 64 62 

 2544 130 94 101 105 96 97 76 75 71 70 66 

 2545 125 99 95 87 80 79 70 75 72 69 70 

 2546 146 100 103 96 90 85 84 71 70 74 73 

 2547 122 94 81 90 81 73 70 64 63 66 59 

 2548 134 105 99 93 90 83 77 73 71 74 76 

 2549 129 102 94 98 88 82 80 70 71 71 58 55a

 49 

 2550 126 101 92 82 78 75 68 67 66 65 61 

 2551 138 97 95 95 84 77 73 64 61 56 54 

MEAN 130 101 95 91 85 81 76 72 68 69 65 59 56 54 49

S.D. 6.2 4.8 6.5 5.6 5.4 5.4 5.4 5.4 3.8 7.5 7.1 3.5 6.7 5.7 0.0

 N 26 26 26 26 26 26 26 26 26 26 25 3 3 4 1

 SF=Spilled Feeder 

a

Animal was pregnant but had total litter loss. Recording of food consumption was resumed.
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 313 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 123 93 88 85 74 70 70 68 67 62 51 

 3527 126 95 89 85 76 74 70 72 64 64 56 

 3528 123 98 91 85 75 78 72 76 69 78 50 

 3529 117 88 85 82 76 72 70 67 67 65 48 

 3530 138 105 91 95 85 81 77 77 64 69 57 

 3531 150 101 93 87 79 74 68 68 64 62 54 

 3532 121 91 88 82 75 76 71 69 61 67 51 

 3533 120 97 87 86 75 72 65 59 57 59 41 

 3534 122 103 93 92 84 79 77 76 65 67 55 

 3535 131 100 96 90 87 76 75 69 69 70 68 

 3536 130 92 92 81 76 76 76 71 62 66 64 61a

 56 

 3537 120 96 97 83 78 76 74 67 67 74 73 

 3538 130 102 96 91 84 79 73 72 66 74 71 57 54 52 

 3539 125 96 88 88 87 77 72 71 68 67 63 55 46 51 

 3540 154 120 111 102 94 93 92 86 77 74 80 

 3541 140 104 102 90 84 82 74 75 74 84 60 

 3542 SF 105 103 90 93 83 85 72 67 74 66 

 3543 127 97 91 87 81 80 71 66 68 77 61 

 3544 128 103 95 91 87 80 77 75 76 71 71 

 3545 130 103 93 92 84 84 70 74 64 72 66 

 3546 142 112 110 102 86 83 77 73 73 74 80 

 3547 128 100 92 94 80 82 81 75 69 74 65 

 3548 136 102 93 92 90 77 73 69 68 77 53 51a

 45 

 3549 114 93 90 87 104 82 83 71 70 67 65 

 3550 126 109 104 84 79 80 76 70 70 71 74 55 53 51 

 3551 138 105 93 90 79 77 72 72 70 65 65 

MEAN 130 100 94 89 83 79 75 72 67 70 62 56 51 53 51

S.D. 9.7 7.0 6.7 5.4 7.2 4.7 5.7 4.8 4.4 5.9 9.8 0.9 4.5 4.4 7.8

 N 25 26 26 26 26 26 26 26 26 26 26 3 3 5 2

 SF=Spilled Feeder 

a

Animal was pregnant but had total litter loss. Recording of food consumption was resumed.
23-JUN-2009 18:38

Huntingdon Life Sciences 00-4206 PAGE 314 

 

 APPENDIX M 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 122 93 93 88 84 80 76 73 66 63 58 

 4527 132 SF 94 86 85 80 75 73 66 61 65 

 4528 145 100 98 90 87 84 76 72 66 69 64 

 4529 128 90 89 81 80 78 74 66 64 65 60 

 4530 124 83 91 82 86 80 78 78 67 66 65 

 4531 123 95 88 81 74 76 74 65 63 60 59 

 4532 131 96 99 101 90 91 88 75 70 74 73 

 4533 129 90 90 91 87 86 81 76 75 77 67 

 4534 116 85 84 84 81 78 73 71 65 68 62 

 4535 135 92 90 85 82 79 78 74 66 63 66 

 4536 119 88 91 85 79 79 76 70 67 68 69 

 4537 125 93 93 85 86 79 78 76 71 74 72 

 4538 130 98 92 87 84 78 77 71 71 65 62 

 4539 135 102 93 92 82 88 91 75 68 76 58 

 4540 135 91 88 89 80 86 83 77 69 67 67 

 4541 127 97 88 85 85 77 73 68 68 65 60 60 53 58 

 4542 149 111 104 94 91 85 80 77 68 71 69 

 4543 144 101 92 89 82 73 77 66 68 64 64 

 4544 145 101 99 91 85 80 76 70 66 69 68 

 4545 126 96 86 86 81 72 72 69 65 64 61 

 4546 131 101 97 88 84 80 82 70 70 74 75 

 4547 137 102 98 90 84 86 74 62 68 64 65 60b 

 4548 136 102 89 98 86 78 76 75 68 67 65 

 4549 132 104 91 90 79 84 

 4550 134 100 94 87 82 76 73 65 65 66 62 

 4551 154 112 98 96 92 89 79 77 75 71 69 

MEAN 133 97 93 89 84 81 78 72 68 68 65 60 53 58 

S.D. 9.2 7.1 4.8 4.9 4.0 4.8 4.6 4.4 2.8 4.7 4.6 0.4 0.0 0.0 

 N 26 25 26 26 26 26 25 25 25 25 25 2 1 1 

 SF=Spilled Feeder 

bFood consumption continued until the last day before delivery because this animal had no Gestation Day 0 (but the animal was found to

be pregnant).
23-JUN-2009 18:45

Huntingdon Life Sciences 00-4206 PAGE 315 

 

 APPENDIX N 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 1526 88 76 68 60 73 

 1527 DPP 65 74 67 58 66 

 1528 74 75 69 57 69 

 1529xPNMD 

 1530 65 73 68 67 68 

 1531 64 66 63 56 62 

 1532x NP 62 68 66 64 65 

 1533 72 74 69 60 69 

 1534 73 77 76 70 74 

 1535x NP 80 76 60 72 

 1536 80 80 74 52 71 

 1537 69 82 76 68 74 

 1538 67 68 65 59 65 

 1539 75 71 70 60 69 

 1540 79 76 72 65 73 

 1541x NP 77 74 69 54 69 

 1542 83 71 64 59 69 

 1543 71 72 69 76 72 

 1544 73 79 74 57 71 

 1545 71 74 67 61 68 

 1546 77 77 75 63 73 

 1547 80 81 73 66 75 

 1548xNNMD 

 1549 78 75 71 65 72 

 1550xNNMD 

 1551 72 67 66 63 67 

MEAN 74 74 70 62 70

S.D. 6.3 4.3 3.9 5.7 3.4

 N 20 20 20 20 20

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 

 x=EXCLUDED FROM MEAN 

 SF=Spilled Feeder 
23-JUN-2009 18:45

Huntingdon Life Sciences 00-4206 PAGE 316 

 

 APPENDIX N 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 2526 77 80 70 63 72 

 2527xNNMD 

 2528 70 58 69 64 65 

 2529 66 68 67 56 64 

 2530 70 73 72 62 69 

 2531 77 78 71 60 72 

 2532x NP 61 65 63 63 63 

 2533 70 72 69 64 69 

 2534 75 80 73 64 73 

 2535 73 75 71 56 69 

 2536 77 80 75 66 75 

 2537 85 89 84 70 82 

 2538xNNMD 

 2539 76 84 78 59 74 

 2540 92 98 82 72 86 

 2541 69 74 68 57 67 

 2542xNNMD 

 2543 61 68 56 61 61 

 2544 79 76 67 62 71 

 2545 77 88 71 68 76 

 2546 88 94 79 70 83 

 2547 62 59 66 57 61 

 2548 70 68 70 66 68 

 2549 TLL 76 76 68 63 71 

 2550 72 69 69 62 68 

 2551 70 75 71 47 66 

MEAN 74 76 71 62 71

S.D. 7.6 10.0 6.0 5.7 6.5

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:45

Huntingdon Life Sciences 00-4206 PAGE 317 

 

 APPENDIX N 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 3526x NP 59 62 57 57 59 

 3527 86 76 68 57 72 

 3528 75 73 71 63 71 

 3529 70 75 70 65 70 

 3530 78 77 73 60 72 

 3531 72 80 78 68 74 

 3532 72 74 70 67 71 

 3533 65 63 61 58 62 

 3534 81 82 73 68 76 

 3535 66 65 65 55 63 

 3536 TLL 71 71 65 16 56 

 3537 73 70 69 61 68 

 3538xNNMD 

 3539xNNMD 

 3540 90 78 47 72 

 3541 80 80 71 65 74 

 3542 73 76 72 67 72 

 3543 73 74 71 66 71 

 3544 77 75 71 65 72 

 3545 68 71 68 64 68 

 3546 74 74 72 64 71 

 3547 82 81 78 67 77 

 3548 TLL 73 79 68 62 71 

 3549 74 87 75 64 75 

 3550xNNMD 

 3551 75 76 71 63 71 

MEAN 74 76 71 61 70

S.D. 5.3 6.3 4.2 11.1 4.9

 N 21 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:45

Huntingdon Life Sciences 00-4206 PAGE 318 

 

 APPENDIX N 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 4526 71 69 71 66 69 

 4527 72 69 67 62 67 

 4528 73 72 72 63 70 

 4529 68 74 67 61 67 

 4530x NP 77 75 72 42 67 

 4531 64 65 63 61 63 

 4532 78 86 81 65 78 

 4533 79 88 83 70 80 

 4534 74 75 71 67 72 

 4535 71 75 71 68 71 

 4536 71 74 74 63 70 

 4537 89 77 75 70 78 

 4538 72 75 68 60 69 

 4539x NP 77 78 70 50 69 

 4540 85 87 71 62 76 

 4541xNNMD 

 4542 74 80 80 70 76 

 4543 68 71 68 62 67 

 4544 78 80 73 67 74 

 4545 73 73 73 69 72 

 4546 87 76 77 70 77 

 4547xPNMD 

 4548 67 74 35 64 60 

 4549 DIED 

 4550 69 73 71 63 69 

 4551 71 85 77 73 76 

MEAN 74 76 71 65 72

S.D. 6.5 6.2 9.6 3.7 5.1

 N 21 21 21 21 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN 
23-JUN-2009 18:46

Huntingdon Life Sciences 00-4206 PAGE 319 

 

 APPENDIX O 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 1526 107 87 148 184 328 171 

 1527 DPP 

 1528 105 114 139 149 256 153 

 1529 PNMD a 98 137 174 256 166 

 1530 99 100 150 167 312 166 

 1531 105 93 138 168 304 161 

 1532x NP 

 1533 113 109 146 169 292 166 

 1534 111 122 140 197 343 183 

 1535x NP 

 1536 101 115 146 152 213 146 

 1537 103 127 154 176 308 174 

 1538 83 115 136 183 316 166 

 1539 113 121 141 174 316 173 

 1540 123 125 144 170 351 183 

 1541x NP 

 1542 94 106 134 164 302 160 

 1543 115 122 158 210 373 196 

 1544 97 119 143 155 306 164 

 1545 94 100 129 156 271 150 

 1546 106 126 147 163 298 168 

 1547 125 155 149 176 348 191 

 1549 110 123 150 177 323 177 

 1551 109 126 156 177 323 178 

MEAN 106 115 144 172 307 169

S.D. 10.3 15.3 7.7 14.7 37.0 12.8

 N 19 20 20 20 20 20

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE x=EXCLUDED FROM MEAN 

a

Feed consumption data was not collected on Lactation Day 1 due to technician error.
23-JUN-2009 18:46

Huntingdon Life Sciences 00-4206 PAGE 320 

 

 APPENDIX O 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 2526 102 126 148 176 307 172 

 2528 59 128 144 168 316 163 

 2529 82 111 CF 180 324 174 

 2530 102 116 125 168 269 156 

 2531 74 99 131 170 307 156 

 2532x NP 

 2533 86 110 141 175 309 164 

 2534 90 106 104 140 221 132 

 2535 104 101 99 154 276 147 

 2536 90 102 133 165 303 159 

 2537 113 87 121 190 279 158 

 2539 99 101 136 179 283 160 

 2540 93 132 147 208 388 194 

 2541 74 85 125 165 325 155 

 2543 108 108 112 174 339 168 

 2544 64 73 86 101 185 102 

 2545 119 127 124 164 308 168 

 2546 123 133 147 195 368 193 

 2547 105 120 134 194 333 177 

 2548 71 91 139 189 258 150 

 2549 TLL 

 2550 95 110 129 186 274 159 

 2551 80 99 133 203 297 162 

MEAN 92 108 128 174 299 160

S.D. 17.6 16.3 16.8 23.3 45.1 19.5

 N 21 21 20 21 21 21

 NP=NOT PREGNANT TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

 CF=Contaminated Feeder
23-JUN-2009 18:46

Huntingdon Life Sciences 00-4206 PAGE 321 

 

 APPENDIX O 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 3526x NP 

 3527 110 123 137 175 322 173 

 3528 60 77 106 129 240 122 

 3529 97 106 139 162 299 161 

 3530 99 106 126 162 325 164 

 3531 103 114 135 190 331 175 

 3532 108 108 133 156 279 157 

 3533 91 96 137 174 316 163 

 3534 122 120 149 178 315 177 

 3535 97 110 131 125 308 154 

 3536 TLL 

 3537 101 121 135 166 259 156 

 3540 115 113 129 171 313 168 

 3541 118 115 147 168 290 168 

 3542 82 140 140 180 313 171 

 3543 100 121 146 162 293 164 

 3544 122 124 150 185 316 179 

 3545 136 134 161 170 278 176 

 3546 115 123 145 181 331 179 

 3547 74 105 153 176 303 162 

 3548 TLL 

 3549 107 107 134 131 333 162 

 3551 86 94 113 156 265 143 

MEAN 102 113 137 165 301 164

S.D. 17.9 14.1 13.0 18.2 26.1 13.5

 N 20 20 20 20 20 20

 NP=NOT PREGNANT TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
23-JUN-2009 18:46

Huntingdon Life Sciences 00-4206 PAGE 322 

 

 APPENDIX O 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 4526 90 108 140 171 262 154 

 4527 95 114 133 150 275 154 

 4528 74 80 121 150 287 142 

 4529 78 87 136 154 311 153 

 4530x NP 

 4531 91 109 132 168 266 154 

 4532 84 103 145 182 329 169 

 4533 124 112 141 177 323 175 

 4534 104 117 138 155 243 151 

 4535 88 103 124 173 314 160 

 4536 105 112 141 182 330 174 

 4537 116 112 136 179 296 168 

 4538 119 124 143 187 312 177 

 4539x NP 

 4540 102 112 131 169 317 166 

 4542 79 94 131 171 326 160 

 4543 108 114 145 174 288 166 

 4544 117 116 134 184 310 172 

 4545 103 120 141 155 253 155 

 4546 114 124 146 185 266 167 

 4547 PNMD 99 98 134 163 312 161 

 4548 102 109 139 183 262 159 

 4550 103 114 127 185 284 163 

 4551 106 123 139 169 239 156 

MEAN 100 109 136 171 291 162

S.D. 14.0 11.4 6.8 12.0 29.0 9.0

 N 22 22 22 22 22 22

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE x=EXCLUDED FROM MEAN 
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 323 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 /E D D D /E D D D /E D D D /E M D D /E D D D /P 14 1 5 1 5 4.0 

 1527 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 1528 D /E M D D /E D D D /E M D D /E D D D D D D D 15 0 4 2 3 4.0 

 1529 D /E E D D D /E E E D D D D /E M D D D /E E D 12 0 8 1 3 5.7 

 1530 /E D D D /E M D D D D D D D D D D D D D D /E 17 0 3 1 1 4.0+

 

 1531 D /E M D D D /E M D D D D D D D D D D D D D 17 0 2 2 1 5.0+

 

 1532 D D /E D D D /E D D D /E D D /E E M D D /E M D 13 0 6 2 4 4.0 

 1533 D D D /E D D D /E M D D /E M D D /E E M D D /P 12 1 5 3 4 4.3 

 1534 D /E D D D /E D D D /E M D D /E M D D /E M D D 13 0 5 3 4 4.0 

 1535 /P E D D D /E E D D D D /P D D /E M D D D /E D 13 2 5 1 4 4.8 

 1536 /E D D D /E D D D /E M D D /E M D D /P P D D D 13 2 4 2 4 4.0 

 1537 D /E D D D /E D D D /E M D D /E D D D /E E D D 14 0 6 1 4 4.0 

 1538 D D D /E D D D /E D D D /E E M D D D D /E M D 14 0 5 2 3 5.0 

 1539 /E D D D /E D D D /E M D D D /E M D D D /E M D 13 0 5 3 4 4.5 

 1540 D D /E D D D /E D D D /E D D D /E D D D /E M D 15 0 5 1 4 4.0 

 1541 D D D /E M D D /E D D D /E D D D /E M D D /E D 14 0 5 2 4 4.0 

 1542 D D /E M D D /E D D D /E M D D /E D D D /E M D 13 0 5 3 4 4.0 

 1543 D D /E D D D /E D /E D /E D D D /E D D D /E D D 15 0 6 0 5 3.2 

 1544 /E D D D /E M D D D D D /E E M D D /E M D D /E 12 0 6 3 4 5.0 

 1545 D /E D D D /E D D D /E E D D D /E D D D /E D D 15 0 6 0 4 4.3 

 1546 D /E M D D /E D D D /E D D D /E D D D /E D D D 15 0 5 1 4 4.0 

 1547 D /P E D D D /E D /E D /E D D D /E M D D /E D D 13 1 6 1 5 3.4 

 1548 D D D /E M D D D /E M D D /E M D D /E M D D /E 12 0 5 4 4 4.3 

 1549 D /E D D D /E M D D /E M D D /E M D D /E E D D 12 0 6 3 4 4.0 

 1550 D D /E D D D /E D D D /E M D D /E D D D /E D D 15 0 5 1 4 4.0 

 1551 D D /P E M D D D /E M D D /E E M D D D /E M D 11 1 5 4 3 5.3 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+

Period of pseudopregnancy excluded.
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 324 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 D /E D D D /E M D D /E D D D /E D D D /E M D D 14 0 5 2 4 4.0 

 2527 D D D D D D /E D D D /E D D D /E D D D /E M D 16 0 4 1 3 4.0 

 2528 D D D D D D /E M D D /E D D D /E D D D /P E M 14 1 4 2 3 4.0 

 2529 /E D D D /E D D D /E D D D /E M D D /E D D /E E 13 0 7 1 5 3.8 

 2530 D D D D /E D D D /P E D D D /E M D D D D /P D 15 2 3 1 3 5.0 

 2531 /E D D D /E D D D /E D D D D /E D D D D D D D 17 0 4 0 3 4.3 

 2532 D D D /P E M D D D D D D D D D D D D D D D 18 1 1 1 - - +

 

 2533 D D D D D D /E D D D /E D D D /E D D D /E M D 16 0 4 1 3 4.0 

 2534 D D D D D D /E M D D /E M D D /E D D D /E D D 15 0 4 2 3 4.0 

 2535 D D D /E D D D D D D D D D D D D D D D D D 20 0 1 0 - - +

 

 2536 D D D D D D /E M D D /E D D D /E M D D /E E D 14 0 5 2 3 4.0 

 2537 D /E D D D /E M D D /E M D D /E D D D D /E E D 13 0 6 2 4 4.3 

 2538 D D D D D D D D D D D /E E M D D /E D D D /E 16 0 4 1 2 4.5+

 

 2539 D D D D D D /E D D D /P E D D D /E E D D D /P 15 2 4 0 3 4.7 

 2540 /E D D D /E M D D /E M D D /E M D D /E M D D /E 11 0 6 4 5 4.0 

 2541 D D D D D D /E E D D D /E M D D D D /E D D D 16 0 4 1 2 5.5 

 2542 D D D D D D /E D D D /E M D D /E M D D /E E D 14 0 5 2 3 4.0 

 2543 D /E D D /P E M D D /E D D D /E D D /P E E M D 11 2 6 2 4 3.8 

 2544 /M D D /E D D D /E M D D /E E M D D /E D D D /E 12 0 6 3 5 4.0 

 2545 D D D /E D D D /E D D D /E D D D /E D D D /E E 15 0 6 0 4 4.0 

 2546 D D D D D D /E D D D /E D D D /E D D D /E D D 17 0 4 0 3 4.0 

 2547 D D D /E D D D /E E D D /E D D D /E D D D /E D 15 0 6 0 4 4.0 

 2548 /E D D D /E D D D D D D D D D D D D D D D D 19 0 2 0 1 4.0+

 

 2549 /E E D D D D /E D D D /P E D D D D /E D D D /E 14 1 6 0 4 5.0 

 2550 D D D /E D D D /E D D D /E D D D /E D D D /P E 15 1 5 0 4 4.0 

 2551 D /E D D D /E D D D /E D D D /E D D D /E E D D 15 0 6 0 4 4.0 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+

Period of pseudopregnancy excluded.
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 325 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 D D /E M D D /E E D D /E D D /E E D D D /E M D 12 0 7 2 4 4.0 

 3527 D D D D D /E D D D /E D D D /E M D D /E D D D 16 0 4 1 3 4.0 

 3528 D /E E D D D D D D D D D D D D D D D /E M D 17 0 3 1 - - +

 

 3529 /E D D D /E M D D /E D D D /E D D D /E D D D /P 14 1 5 1 5 4.0 

 3530 D D /E D D D /P E M D D /P E D D D /E E M D D 12 2 5 2 3 4.7 

 3531 D /E D D D /E D D D /E D D D /E D D D /E D D D 16 0 5 0 4 4.0 

 3532 D D /E D D D D /E D D D /P M M D D /E E M D D 13 1 4 3 3 4.7 

 3533 D D D D /P E M D D D D /E M D D D /E E M D D 13 1 4 3 2 6.0 

 3534 D /E M D D /E D D D /E M D D /E M D D /E M D D 12 0 5 4 4 4.0 

 3535 /E M D D /P E D D D /E E D D D /E E M D D D D 12 1 6 2 3 4.7 

 3536 D D /E M D D /E M D D /E D D D /E D D D /E M D 13 0 5 3 4 4.0 

 3537 D D D /E D D D /E E M D D D D D D D D D D D 17 0 3 1 1 4.0+

 

 3538 D /E E M D D /E E D D D D D D D D D D D D D 16 0 4 1 1 5.0+

 

 3539 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 3540 D /E D D D /E M D D /E M D D /E M D /E E D D D 12 0 6 3 4 3.8 

 3541 D D /E E D D D D D D D D D D /E D /E E D D D 16 0 5 0 1 2.0+

 

 3542 /E M D D /E D D D D D D D D D /E D D D /E D D 16 0 4 1 3 6.0 

 3543 D D D /E D D D D D D D D D D D D D D /E D D 19 0 2 0 - - +

 

 3544 /E D D /P E D D D /E D D /P E D D D /E D D D /E 13 2 6 0 5 4.0 

 3545 D D D /E D D D D D D D D D D D D /E E D D D 18 0 3 0 - - +

 

 3546 /P E M D D /E D D D /P E D D D D D D D D D D 15 2 3 1 2 4.5+

 

 3547 D D D /E D D D /E D D D /E D D /E E D D D /E D 15 0 6 0 4 4.0 

 3548 /E D D D /E D D D D D D D D /E D D /E D D /E E 15 0 6 0 4 4.8 

 3549 D D D /E D D D /E D D D /E D D D /E E D D D /E 15 0 6 0 4 4.3 

 3550 D D /E D D D /E M D D /E D D D D D D D D D D 17 0 3 1 2 4.0+

 

 3551 D D D /P E D D D /E E D D D D /E D D D /E E D 14 1 6 0 3 5.0 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+

Period of pseudopregnancy excluded.
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 326 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 D /E M D D /E D D D /E M D D /E M D D /P E E D 11 1 6 3 4 4.0 

 4527 D D /E E M D D D /E E D D D /E E M D D D /E D 12 0 7 2 3 5.7 

 4528 D D D /E D D D /E D D D /E D D D /E D D D /P E 15 1 5 0 4 4.0 

 4529 D D D /E M D D /E M D D /E M D D /E M D D /E E 11 0 6 4 4 4.0 

 4530 D /E D D D /E D D D /E D D D /E M D D /E M D D 14 0 5 2 4 4.0 

 4531 D D /E D D D /E D D D /E D D D /E D D D /E E D 15 0 6 0 4 4.0 

 4532 D D /E D D D /E M D D /E D D D /E D D D /E D D 15 0 5 1 4 4.0 

 4533 D D D /E D D D /E D D D /E M D /E E E E E D D 12 0 8 1 3 3.7 

 4534 D D D /E M D D /E M D D /E D D D /E D D D D /E 14 0 5 2 4 4.3 

 4535 D /E M D D /E D D /P E D D D /E D D /E E D D D 13 1 6 1 4 3.8 

 4536 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0 

 4537 /P E D D D /E E D D D /P E M D D /E E D D D D 12 2 6 1 3 5.0 

 4538 /E M D D /E D D D /E M D D /E D D D /E D D D /E 13 0 6 2 5 4.0 

 4539 D D D /E D D D /E D D D D D D D D D D D D D 19 0 2 0 1 4.0+

 

 4540 D D D /P E D D D D /E D D D /P E D D D /E D /E 14 2 5 0 4 4.3 

 4541 /E D D D /E D D D /E D D D /E D D D /E E E D D 14 0 7 0 4 4.0 

 4542 D /P E D D D /P E D D D D /E D D D /E E E D D 13 2 6 0 3 5.0 

 4543 /E M D D /E M D D /E M D D D /M D D D /E M D D 12 0 4 5 4 4.3 

 4544 /E M D D /E E M D D /E M D /E D /E D D D D /E E 10 0 8 3 5 3.8 

 4545 D /E M D D /E E D D D /E D D D /E D D D /E D D 14 0 6 1 4 4.3 

 4546 D D /E E D D /E D D D /E D D D /E M D D /E D D 14 0 6 1 4 4.0 

 4547 /E D D D /E D D D /E D D D /E D D D /E M D D /E 14 0 6 1 5 4.0 

 4548 D /E D D D /E D D D /P E D D /P E D D D /E D D 14 2 5 0 4 4.3 

 4550 D D /E D D D /E D D D /E M D D /E D /E D /E D D 14 0 6 1 5 3.2 

 4551 /E D D D /E D D D /E D D /E E D D D D D D D D 16 0 5 0 3 3.7 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+

Period of pseudopregnancy excluded.
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 327 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 1526 S 

 1527 D D D S 

 1528 D D D C 

 1529 D D D D D D D D D D D D D D 

 1530 D D D S 

 1531 C 

 1532 D E E C 

 1533 C 

 1534 C 

 1535 C 

 1536 E D D D P S 

 1537 D E C 

 1538 D S 

 1539 D C 

 1540 D C 

 1541 D D C 

 1542 D C 

 1543 D E E D D E S 

 1544 M D D C 

 1545 D C 

 1546 P C 

 1547 D C 

 1548 M D D D D D D D D D D D D D 

 1549 E C 

 1550 D E D D D E M D D E D D D E 

 1551 D P C 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 328 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 2526 E C 

 2527 D E D D D E D D D E D E E E 

 2528 D D S 

 2529 D D E S 

 2530 D D D D D D D D C 

 2531 D D C 

 2532 D D C 

 2533 D C 

 2534 D E C 

 2535 S 

 2536 D P E E C 

 2537 C 

 2538 M D D E D D D E E D D D D D 

 2539 E C 

 2540 E M D C 

 2541 E C 

 2542 D E D D E E E E E E E E E E 

 2543 D C 

 2544 M D C 

 2545 E E E C 

 2546 D C 

 2547 D D D S 

 2548 D D C 

 2549 E C 

 2550 C 

 2551 D E C 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 329 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 3526 D S 

 3527 S 

 3528 D E E D D C 

 3529 C 

 3530 D C 

 3531 P S 

 3532 D C 

 3533 D P S 

 3534 D C 

 3535 D D D D D D D C 

 3536 D E S 

 3537 D D S 

 3538 D E M D D E M D D E D D D E 

 3539 E E D D D E D D D D D D D D 

 3540 E C 

 3541 S 

 3542 D C 

 3543 D S 

 3544 D D D S 

 3545 E S 

 3546 D D D D S 

 3547 D D S 

 3548 D D D E C 

 3549 D D D P C 

 3550 D D D D D D D D D D D D D D 

 3551 D C 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
08-SEP-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 330 

 

 APPENDIX P 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 4526 D D P E D D D S 

 4527 D D P S 

 4528 E D D D C 

 4529 M D D P C 

 4530 E E M D C 

 4531 D D C 

 4532 D E D D D S 

 4533 S 

 4534 E E D D D C 

 4535 S 

 4536 D C 

 4537 E E D D D D C 

 4538 D C 

 4539 D S 

 4540 S 

 4541 D D E E E D E E E D E E E E 

 4542 D P C 

 4543 P S 

 4544 S 

 4545 D D E D D D D S 

 4546 D E C 

 4547 D D D D D D D D D D D D D D 

 4548 D P S 

 4550 D E M D D E M D D P C 

 4551 E C 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 331 

 

 APPENDIX Q 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Date of Date of Date of 

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 1026 + P 28-OCT-02 29-OCT-02 20-NOV-02

 1527 1027 + DPP,P 28-OCT-02 1-NOV-02 23-NOV-02

 1528 1028 + P 28-OCT-02 1-NOV-02 23-NOV-02

 1529 1029 - PNMD 28-OCT-02 27-NOV-02

 1530 1030 + P 28-OCT-02 1-NOV-02 23-NOV-02

 1531 1031 + P 28-OCT-02 29-OCT-02 20-NOV-02

 1532 1032 + NP 28-OCT-02 1-NOV-02 

 1533 1033 + P 28-OCT-02 29-OCT-02 20-NOV-02

 1534 1034 + P 28-OCT-02 29-OCT-02 20-NOV-02

 1535 1035 + NP 28-OCT-02 29-OCT-02 

 1536 1036 + P 28-OCT-02 3-NOV-02 25-NOV-02

 1537 1037 + P 28-OCT-02 31-OCT-02 22-NOV-02

 1538 1038 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1539 1039 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1540 1040 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1541 1041 + NP 28-OCT-02 31-OCT-02 

 1542 1042 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1543 1043 + P 28-OCT-02 4-NOV-02 26-NOV-02

 1544 1044 + P 28-OCT-02 1-NOV-02 23-NOV-02

 1545 1045 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1546 1046 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1547 1047 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1548 1048 - NNMD 28-OCT-02 

 1549 1049 + P 28-OCT-02 30-OCT-02 21-NOV-02

 1550 1050 - NNMD 28-OCT-02 

 1551 1051 + P 28-OCT-02 31-OCT-02 22-NOV-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE P=PREGNANT 
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 332 

 

 APPENDIX Q 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Date of Date of Date of 

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 2026 + P 28-OCT-02 30-OCT-02 21-NOV-02

 2527 2027 - NNMD 28-OCT-02 

 2528 2028 + P 28-OCT-02 31-OCT-02 22-NOV-02

 2529 2029 + P 28-OCT-02 1-NOV-02 23-NOV-02

 2530 2030 + P 28-OCT-02 6-NOV-02 28-NOV-02

 2531 2031 + P 28-OCT-02 31-OCT-02 22-NOV-02

 2532 2032 + NP 28-OCT-02 31-OCT-02 

 2533 2033 + P 28-OCT-02 30-OCT-02 21-NOV-02

 2534 2034 + P 28-OCT-02 31-OCT-02 23-NOV-02

 2535 2035 + P 28-OCT-02 29-OCT-02 20-NOV-02

 2536 2036 + P 28-OCT-02 2-NOV-02 24-NOV-02

 2537 2037 + P 28-OCT-02 29-OCT-02 20-NOV-02

 2538 2038 - NNMD 28-OCT-02 

 2539 2039 + P 28-OCT-02 30-OCT-02 21-NOV-02

 2540 2040 + P 28-OCT-02 1-NOV-02 23-NOV-02

 2541 2041 + P 28-OCT-02 30-OCT-02 21-NOV-02

 2542 2042 - NNMD 28-OCT-02 

 2543 2043 + P 28-OCT-02 30-OCT-02 21-NOV-02

 2544 2044 + P 28-OCT-02 31-OCT-02 23-NOV-02

 2545 2045 + P 28-OCT-02 1-NOV-02 23-NOV-02

 2546 2046 + P 28-OCT-02 30-OCT-02 21-NOV-02

 2547 2047 + P 28-OCT-02 1-NOV-02 24-NOV-02

 2548 2048 + P 28-OCT-02 31-OCT-02 22-NOV-02

 2549 2049 + TLL,P 28-OCT-02 30-OCT-02 22-NOV-02

 2550 2050 + P 28-OCT-02 29-OCT-02 20-NOV-02

 2551 2051 + P 28-OCT-02 31-OCT-02 22-NOV-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS P=PREGNANT 
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 333 

 

 APPENDIX Q 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Date of Date of Date of 

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 3026 + NP 28-OCT-02 30-OCT-02 

 3527 3027 + P 28-OCT-02 29-OCT-02 20-NOV-02

 3528 3028 + P 28-OCT-02 3-NOV-02 24-NOV-02

 3529 3029 + P 28-OCT-02 29-OCT-02 19-NOV-02

 3530 3030 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3531 3031 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3532 3032 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3533 3033 + P 28-OCT-02 31-OCT-02 22-NOV-02

 3534 3034 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3535 3035 + P 28-OCT-02 5-NOV-02 27-NOV-02

 3536 3036 + TLL,P 28-OCT-02 31-OCT-02 24-NOV-02

 3537 3037 + P 28-OCT-02 31-OCT-02 21-NOV-02

 3538 3038 - NNMD 28-OCT-02 

 3539 3039 - NNMD 28-OCT-02 

 3540 3040 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3541 3041 + P 28-OCT-02 29-OCT-02 20-NOV-02

 3542 3042 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3543 3043 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3544 3044 + P 28-OCT-02 1-NOV-02 23-NOV-02

 3545 3045 + P 28-OCT-02 30-OCT-02 21-NOV-02

 3546 3046 + P 28-OCT-02 2-NOV-02 24-NOV-02

 3547 3047 + P 28-OCT-02 31-OCT-02 22-NOV-02

 3548 3048 + TLL,P 28-OCT-02 2-NOV-02 24-NOV-02

 3549 3049 + P 28-OCT-02 2-NOV-02 24-NOV-02

 3550 3050 - NNMD 28-OCT-02 

 3551 3051 + P 28-OCT-02 30-OCT-02 21-NOV-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS P=PREGNANT 
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 334 

 

 APPENDIX Q 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Date of Date of Date of 

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 4026 + P 28-OCT-02 5-NOV-02 27-NOV-02

 4527 4027 + P 28-OCT-02 1-NOV-02 23-NOV-02

 4528 4028 + P 28-OCT-02 2-NOV-02 24-NOV-02

 4529 4029 + P 28-OCT-02 2-NOV-02 24-NOV-02

 4530 4030 + NP 28-OCT-02 2-NOV-02 

 4531 4031 + P 28-OCT-02 31-OCT-02 22-NOV-02

 4532 4032 + P 28-OCT-02 3-NOV-02 25-NOV-02

 4533 4033 + P 28-OCT-02 29-OCT-02 20-NOV-02

 4534 4034 + P 28-OCT-02 3-NOV-02 24-NOV-02

 4535 4035 + P 28-OCT-02 29-OCT-02 20-NOV-02

 4536 4052 + P 28-OCT-02 30-OCT-02 21-NOV-02

 4537 4037 + P 28-OCT-02 4-NOV-02 26-NOV-02

 4538 4038 + P 28-OCT-02 30-OCT-02 20-NOV-02

 4539 4039 + NP 28-OCT-02 30-OCT-02 

 4540 4040 + P 28-OCT-02 29-OCT-02 20-NOV-02

 4541 4041 - NNMD 28-OCT-02 

 4542 4042 + P 28-OCT-02 31-OCT-02 22-NOV-02

 4543 4043 + P 28-OCT-02 30-OCT-02 21-NOV-02

 4544 4044 + P 28-OCT-02 29-OCT-02 20-NOV-02

 4545 4045 + P 28-OCT-02 5-NOV-02 28-NOV-02

 4546 4046 + P 28-OCT-02 31-OCT-02 22-NOV-02

 4547 4047 - PNMD 28-OCT-02 23-NOV-02

 4548 4048 + P 28-OCT-02 31-OCT-02 22-NOV-02

 4549 N DIED 

 4550 4050 + P 28-OCT-02 8-NOV-02 1-DEC-02

 4551 4051 + P 28-OCT-02 30-OCT-02 21-NOV-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE P=PREGNANT 
23-JUN-2009 19:21

Huntingdon Life Sciences 00-4206 PAGE 335 

 

 APPENDIX R 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF 

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 1526 14 1 15 8 6 8 6 5 5 5 5 5 5 5 5 15 22 

 1527x DPP 14 0 14 3 9 0 0 0 0 0 0 0 0 0 0 15 22 

 1528 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 17 22 

 1529 PNMD 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 15 no day 0

 1530 12 0 12 6 6 5 6 5 5 5 5 5 5 5 5 13 22 

 1531 16 0 16 7 9 7 9 5 5 5 5 5 5 5 5 17 22 

 1532x NP 

 1533 16 0 16 8 8 8 8 5 5 5 5 5 5 5 5 16 22 

 1534 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 14 22 

 1535x NP 

 1536 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 16 22 

 1537 13 0 13 10 3 10 3 7 3 7 3 7 3 7 3 15 22 

 1538 12 0 12 5 7 5 7 5 5 5 5 5 5 5 5 12 22 

 1539 13 0 13 7 6 7 6 5 5 5 5 5 5 5 5 13 22 

 1540 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22 

 1541x NP 

 1542 14 0 14 6 8 6 8 5 5 5 5 5 5 5 5 16 22 

 1543 16 0 16 9 7 9 7 5 5 5 5 5 5 5 5 16 22 

 1544 15 0 15 8 7 8 7 5 5 5 4 5 4 5 4 19 22 

 1545 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 15 22 

 1546 14 0 14 9 5 9 5 5 5 5 5 5 5 5 5 15 22 

 1547 14 0 14 11 3 11 3 7 3 7 3 7 3 7 3 14 22 

 1548xNNMD 

 1549 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 15 22 

 1550xNNMD 

 1551 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22 

MEAN 14.3 0.1 14.3 7.5 6.8 7.4 6.8 5.2 4.8 5.2 4.8 5.2 4.8 5.2 4.8 15.1 22.0 

S.D. 1.3 0.2 1.3 1.8 1.9 1.8 1.9 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 1.6 0.0 

 N 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 19 

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 

 x=EXCLUDED FROM MEAN 

DAY 4 COLUMN = PRECULLING
23-JUN-2009 19:21

Huntingdon Life Sciences 00-4206 PAGE 336 

 

 APPENDIX R 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 2526 12 1 13 5 6 5 6 5 5 4 5 4 5 4 5 14 22 

 2527xNNMD 

 2528 14 1 15 7 7 7 7 5 5 5 5 5 5 5 5 15 22 

 2529 12 0 12 6 6 6 6 5 5 5 5 5 5 5 5 14 22 

 2530 15 0 15 9 6 9 6 5 5 4 5 4 5 4 5 18 22 

 2531 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 16 22 

 2532x NP 

 2533 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 22 

 2534 6 0 6 2 4 2 4 2 4 2 4 2 4 2 4 14 23 

 2535 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 15 22 

 2536 15 1 16 4 11 4 10 4 6 4 6 4 6 4 6 17 22 

 2537 11 0 11 5 6 5 6 5 5 5 5 5 5 5 5 14 22 

 2538xNNMD 

 2539 14 0 14 7 6 7 6 5 5 5 5 5 5 5 5 16 22 

 2540 10 1 11 6 4 6 4 6 4 6 4 6 4 6 4 14 22 

 2541 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 16 22 

 2542xNNMD 

 2543 14 0 14 3 10 3 10 3 7 3 7 3 7 3 7 14 22 

 2544 4 0 4 2 2 2 2 2 2 2 2 2 2 2 2 13 23 

 2545 12 0 12 7 5 7 5 5 5 5 5 5 5 5 5 13 22 

 2546 12 1 13 6 6 6 6 5 5 5 5 5 5 5 5 14 22 

 2547 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 13 23 

 2548 11 0 11 6 5 6 5 5 5 5 5 5 5 5 5 15 22 

 2549 TLL 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0 7 23 

 2550 8 0 8 4 4 4 4 4 4 4 4 4 4 4 4 9 22 

 2551 15 0 15 4 10 4 10 4 6 4 6 4 6 4 6 15 22 

MEAN 11.8 0.2 12.0 5.4 6.2 5.6 6.4 4.5 4.9 4.4 4.9 4.4 4.9 4.4 4.9 14.1 22.2 

S.D. 3.8 0.4 3.8 2.4 2.5 2.1 2.2 1.0 0.9 1.0 0.9 1.0 0.9 1.0 0.9 2.4 0.4 

 N 22 22 22 22 22 21 21 21 21 21 21 21 21 21 21 21 22 

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

DAY 4 COLUMN = PRECULLING
23-JUN-2009 19:21

Huntingdon Life Sciences 00-4206 PAGE 337 

 

 APPENDIX R 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 3526x NP 

 3527 18 0 18 8 10 8 10 5 5 5 5 5 5 5 5 18 22 

 3528 12 0 12 3 8 3 5 3 5 3 5 3 5 3 5 14 21 

 3529 14 0 14 7 7 7 7 5 5 5 5 5 5 5 5 15 21 

 3530 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 15 22 

 3531 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22 

 3532 13 0 13 10 3 10 3 7 3 7 3 7 3 7 3 16 22 

 3533 13 1 14 7 6 7 6 5 5 5 5 5 5 5 5 15 22 

 3534 16 0 16 7 9 7 8 5 5 5 5 5 5 5 5 16 22 

 3535 15 0 15 6 9 6 9 5 5 5 5 5 5 5 5 16 22 

 3536 TLL 2 8 10 0 0 0 0 0 0 0 0 0 0 0 0 17 24 

 3537 17 0 17 9 8 9 8 5 5 5 5 5 5 5 5 17 21 

 3538xNNMD 

 3539xNNMD 

 3540 14 0 14 4 10 4 10 4 6 4 6 4 6 4 6 14 22 

 3541 12 1 13 5 7 5 7 5 5 5 5 5 5 5 5 14 22 

 3542 14 0 14 10 4 10 4 6 4 6 4 6 4 6 4 15 22 

 3543 13 0 13 3 10 3 10 3 7 3 7 3 7 3 7 14 22 

 3544 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 14 22 

 3545 16 0 16 10 6 9 6 5 5 5 5 5 5 5 5 17 22 

 3546 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 17 22 

 3547 14 1 15 6 7 6 7 5 5 5 5 5 5 5 5 15 22 

 3548 TLL 12 1 13 0 0 0 0 0 0 0 0 0 0 0 0 13 22 

 3549 14 0 14 7 7 7 7 5 5 5 5 5 5 5 5 15 22 

 3550xNNMD 

 3551 6 0 6 6 0 6 0 6 0 6 0 6 0 6 0 14 22 

MEAN 13.2 0.5 13.8 6.7 7.1 6.7 6.8 4.9 4.8 4.9 4.8 4.9 4.8 4.9 4.8 15.3 22.0 

S.D. 3.4 1.7 2.4 2.1 2.5 2.0 2.5 0.9 1.3 0.9 1.3 0.9 1.3 0.9 1.3 1.3 0.6 

 N 22 22 22 20 20 20 20 20 20 20 20 20 20 20 20 22 22 

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

DAY 4 COLUMN = PRECULLING
23-JUN-2009 19:21

Huntingdon Life Sciences 00-4206 PAGE 338 

 

 APPENDIX R 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 4526 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22 

 4527 14 0 14 8 6 8 5 5 5 5 5 5 5 5 5 15 22 

 4528 16 0 16 11 5 11 3 6 3 6 3 6 3 6 3 16 22 

 4529 12 0 12 9 3 9 3 7 3 7 3 7 3 7 3 15 22 

 4530x NP 

 4531 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 13 22 

 4532 17 0 17 5 5 5 5 5 5 5 5 5 5 5 5 17 22 

 4533 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22 

 4534 17 0 17 11 6 11 6 5 5 5 5 5 5 5 5 18 21 

 4535 9 0 9 4 5 4 5 4 5 4 5 4 5 4 5 15 22 

 4536 15 0 15 7 8 7 7 5 5 5 5 5 5 5 5 15 22 

 4537 11 0 11 5 6 5 6 5 5 5 5 5 5 5 5 14 22 

 4538 16 0 16 10 5 10 5 5 5 5 5 5 5 5 5 16 21 

 4539x NP 

 4540 12 0 12 6 6 6 6 5 5 5 5 5 5 5 5 14 22 

 4541xNNMD 

 4542 12 0 12 9 3 9 3 7 3 7 3 7 3 7 3 15 22 

 4543 15 0 15 6 9 6 9 5 5 5 5 5 5 5 5 15 22 

 4544 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22 

 4545 13 0 13 10 3 10 3 7 3 7 3 7 3 7 3 15 23 

 4546 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 17 22 

 4547 PNMD 14 0 14 4 10 4 10 4 6 4 6 4 6 4 6 15 no day 0

 4548 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22 

 4549xDIED 

 4550 16 0 16 8 8 7 8 5 5 5 5 5 5 5 5 17 23 

 4551 12 0 12 3 9 3 9 3 7 3 7 3 7 3 7 14 22 

MEAN 14.1 0.0 14.1 7.2 6.5 7.1 6.4 5.1 4.8 5.1 4.8 5.1 4.8 5.1 4.8 15.3 22.0 

S.D. 2.1 0.0 2.1 2.4 2.2 2.4 2.3 0.9 1.0 0.9 1.0 0.9 1.0 0.9 1.0 1.2 0.4 

 N 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 21 

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN 

DAY 4 COLUMN = PRECULLING
23-JUN-2009 19:24

Huntingdon Life Sciences 00-4206 PAGE 339 

 

 APPENDIX S 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 1526 FS MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4

 1527 FD 0 FD 0 MU 1 MU 1 MU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1

 1528 MC 4 MK28 MK28 MK28 MC 4 MC 4 MK28 MK28 MC 4 FK28 FK28 FK28 FC 4 FK28 FK28

 1529 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MC 4 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 

 1530 MK28 MK28 MK28 MD 2 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28

 1531 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4 FC 4 FK28

 1533 MK28 MC 4 MK28 MK28 MC 4 MC 4 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4

 1534 MK28 MK28 MK28 MK28 MC 4 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

 1536 MC 4 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FC 4

 1537 MC 4 MK28 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28

 1538 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FC 4

 1539 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

 1540 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28

 1542 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FC 4

 1543 MC 4 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

 1544 MC 4 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FD 9

 1545 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 MC 4 FK28 FK28 FK28 FC 4 FK28 FK28

 1546 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

 1547 MK28 MK28 MK28 MC 4 MK28 MK28 MC 4 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28

 1549 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FC 4 FK28 FK28 FK28 FC 4 FK28

 1551 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FC 4 FK28 FC 4 FC 4 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE U-UNSCHEDULED SACRIFICE 
23-JUN-2009 19:24

Huntingdon Life Sciences 00-4206 PAGE 340 

 

 APPENDIX S 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 2526 MS FD 0 MM10 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FK28

 2528 MS MK28 MK28 MK28 MC 4 MK28 MK28 MC 4 FC 4 FK28 FK28 FC 4 FK28 FK28 FK28

 2529 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FC 4 FK28

 2530 MC 4 MK28 MK28 MK28 MK28 MC 4 MM14 MC 4 MC 4 FK28 FK28 FK28 FC 4 FK28 FK28

 2531 MK28 MC 4 MC 4 MC 4 MC 4 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

 2533 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FC 4 FC 4 FC 4 FC 4 FK28 FK28 FK28 FK28

 2534 MK28 MK28 FK28 FK28 FK28 FK28

 2535 MC 4 MC 4 MK28 MK28 MC 4 MK28 MC 4 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28

 2536 MS MK28 MK28 MK28 MK28 FK28 FD 3 FC 4 FC 4 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

 2537 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FK28

 2539 FD 0 MK28 MC 4 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

 2540 FZ MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 2541 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

 2543 FD 0 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FC 4 FC 4 FK28 FK28

 2544 MK28 MK28 FK28 FK28

 2545 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

 2546 FS MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28

 2547 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4

 2548 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 2549 FM 2 FM 1

 2550 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 2551 MK28 MK28 MK28 MK28 FM 1 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED M-MISSING K-SCHEDULED SACRIFICE 
23-JUN-2009 19:24

Huntingdon Life Sciences 00-4206 PAGE 341 

 

 APPENDIX S 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 3527 MK28 MC 4 MK28 MC 4 MK28 MK28 MK28 MC 4 FK28 FK28 FC 4 FC 4 FC 4 FC 4 FK28 FC 4 FK28 FK28

 3528 MD 0 MK28 MK28 MK28 FD 4 FK28 FK28 FK28 FK28 FM 2 FM 2 FK28

 3529 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FC 4 FK28 FK28 FC 4 FK28 FK28 FK28

 3530 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FK28

 3531 MK28 MK28 MC 4 MC 4 MK28 MC 4 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FC 4 FK28

 3532 MK28 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 MC 4 FK28 FK28 FK28

 3533 FS MK28 MK28 MC 4 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FK28

 3534 MK28 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FD 4 FC 4 FK28 FK28 FC 4 FC 4

 3535 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28 FC 4 FC 4

 3536 UZ UZ MS MS MS FS FD 0 FS FS FD 0

 3537 MK28 MC 4 MC 4 MK28 FK28 MC 4 MC 4 MK28 MK28 MK28 FC 4 FK28 FC 4 FK28 FC 4 FK28 FK28

 3540 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FC 4 FK28

 3541 MS MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

 3542 MK28 MK28 MC 4 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 FK28 FK28 FK28 FK28

 3543 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FC 4 FK28

 3544 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28

 3545 MK28 MK28 MK28 MD 2 MC 4 MC 4 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FK28 FC 4 FK28

 3546 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FC 4 FK28 FK28 FC 4 FK28 FC 4 FK28

 3547 MS MZ 1 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FC 4

 3548 MS MZ 1 MZ 1 MM 1 MM 1 MM 1 MM 1 MM 1 FZ 1 FZ 1 FM 1 FZ 1 FM 1

 3549 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

 3551 MK28 MK28 MK28 MK28 MK28 MK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED M-MISSING K-SCHEDULED SACRIFICE 
23-JUN-2009 19:24

Huntingdon Life Sciences 00-4206 PAGE 342 

 

 APPENDIX S 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 4526 MC 4 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FK28

 4527 MC 4 MC 4 MK28 MK28 MC 4 MK28 MK28 MK28 FD 3 FK28 FK28 FK28 FK28 FK28

 4528 MK28 MK28 MK28 MK28 MC 4 MC 4 MM 5 MK28 MK28 MC 4 MC 4 FK28 FK28 FM 4 FK28 FM 4

 4529 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

 4531 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28

 4532 MD 1 MK28 MK28 MK28 MK28 FK28 MK28 FD 1 FK28 FK28 FD 1 FK28 FD 1 FK28 FD 1 FD 1 FD 1

 4533 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FC 4

 4534 MK28 MK28 MK28 MC 4 MC 4 MK28 MC 4 MK28 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4

 4535 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 4536 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FD 4 FC 4 FK28 FK28 FC 4

 4537 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

 4538 FD 0 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

 4540 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

 4542 MK28 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28

 4543 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4 FC 4

 4544 MK28 MC 4 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

 4545 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 FK28 FK28 FK28

 4546 MK28 MC 4 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 MC 4 FK28 FK28 FC 4 FK28 FK28 FK28

 4547 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FK28

 4548 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FC 4

 4549 FOUND DEAD 

 4550 MD 4 MC 4 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FK28 FC 4 FK28 FK28 FC 4

 4551 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE D-DIED C-CULLED M-MISSING K-SCHEDULED SACRIFICE 
23-JUN-2009 19:29

Huntingdon Life Sciences 00-4206 PAGE 343 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1526 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 1527 PUP# 1 FOUND DEAD P 

 PUP# 2 FOUND DEAD P 

 PUP# 3 WITHIN NORMAL LIMITS P 

 PUP# 4 WITHIN NORMAL LIMITS P 

 PUP# 5 WITHIN NORMAL LIMITS P 

 PUP# 6 WITHIN NORMAL LIMITS P 

 PUP# 7 WITHIN NORMAL LIMITS P 

 PUP# 8 WITHIN NORMAL LIMITS P 

 PUP# 9 WITHIN NORMAL LIMITS P 

 PUP# 10 WITHIN NORMAL LIMITS P 

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P 

 PUP# 13 WITHIN NORMAL LIMITS P 

 PUP# 14 WITHIN NORMAL LIMITS P 

 1528 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 344 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1528 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 1529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 1530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P 

----------------------------------------------------------------------------------------------------------------------------------- 

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 345 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1530 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 1531 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 1533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 346 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1533 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 PUP# 16 WITHIN NORMAL LIMITS P P 

 1534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1536 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 347 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1536 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

 1537 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1538 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 348 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 

-----------------------------------------------------------------------------------------------------------------------------------

 1539 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 349 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1542 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 1543 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 350 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1544 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P 

 1545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 351 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 1547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:26

Huntingdon Life Sciences 00-4206 PAGE 352 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1549 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 353 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2526 PUP# 2 FOUND DEAD P 

 PUP# 3 WITHIN NORMAL LIMITS P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 2528 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 2529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 354 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2529 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 2530 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 2531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 355 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2531 PUP# 10 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 2533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 2534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 356 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2535 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 2536 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 357 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 2539 PUP# 1 FOUND DEAD P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 2540 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 358 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2540 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 2541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 2543 PUP# 1 FOUND DEAD P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 359 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2544 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 2545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P 

 2546 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 360 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 2548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 2549 PUP# 1 WITHIN NORMAL LIMITS P 

 PUP# 2 WITHIN NORMAL LIMITS P 

 2550 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:30

Huntingdon Life Sciences 00-4206 PAGE 361 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2550 PUP# 5 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 2551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 362 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3527 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P 

 PUP# 17 WITHIN NORMAL LIMITS P P P P P P

 PUP# 18 WITHIN NORMAL LIMITS P P P P P P

 3528 PUP# 1 FOUND DEAD P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P 

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 363 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 

-----------------------------------------------------------------------------------------------------------------------------------

 3529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 3530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 364 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3531 PUP# 5 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 3532 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3533 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 365 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3533 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 3534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 PUP# 16 WITHIN NORMAL LIMITS P P 

 3535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 366 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3535 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

 3536 PUP# 7 FOUND DEAD P 

 PUP# 10 FOUND DEAD P 

 3537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 PUP# 17 WITHIN NORMAL LIMITS P P P P P P

 3540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 367 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3540 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 3541 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 368 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3542 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P 

 3543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3544 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 369 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 3546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 370 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3547 PUP# 2 WITHIN NORMAL LIMITS P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 3548 PUP# 2 WITHIN NORMAL LIMITS P 

 PUP# 3 WITHIN NORMAL LIMITS P 

 PUP# 4 WITHIN NORMAL LIMITS P 

 PUP# 5 WITHIN NORMAL LIMITS P 

 PUP# 6 WITHIN NORMAL LIMITS P 

 PUP# 7 WITHIN NORMAL LIMITS P 

 PUP# 8 WITHIN NORMAL LIMITS P 

 PUP# 9 WITHIN NORMAL LIMITS P 

 PUP# 10 WITHIN NORMAL LIMITS P 

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P 

 PUP# 13 WITHIN NORMAL LIMITS P 

 3549 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:31

Huntingdon Life Sciences 00-4206 PAGE 371 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3549 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 3551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 372 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4526 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 4527 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

----------------------------------------------------------------------------------------------------------------------------------- 

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 373 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P 

 4529 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 374 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 4532 PUP# 1 WITHIN NORMAL LIMITS P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P 

 PUP# 16 WITHIN NORMAL LIMITS P 

 PUP# 17 WITHIN NORMAL LIMITS P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 375 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 4534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 PUP# 17 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 376 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 4536 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 4537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT 
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 377 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4537 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 4538 PUP# 1 FOUND DEAD P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 4540 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 378 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4540 PUP# 12 WITHIN NORMAL LIMITS P P 

 4542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 4543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 379 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4544 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 4545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 380 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 4547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 381 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

 4550 PUP# 1 WITHIN NORMAL LIMITS P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:33

Huntingdon Life Sciences 00-4206 PAGE 382 

 

 APPENDIX T 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
23-JUN-2009 19:41

Huntingdon Life Sciences 00-4206 PAGE 383 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 7.0 S 7.8 7.0 7.1 7.3 7.0 7.7 7.5 7.4 6.6 6.4 6.7 6.6 6.4 6.1 

 1527 5.7 D D 5.3 5.9 4.9 6.1 5.1 6.1 5.5 6.3 5.9 5.8 5.5 5.9 

 1528 7.0 7.0 8.3 6.2 6.7 6.5 7.3 6.8 7.8 7.5 7.1 6.8 6.2 6.9 7.0 6.6 

 1529 7.2 7.2 7.6 7.8 7.9 6.6 7.3 7.7 7.3 7.6 6.4 6.5 7.1 6.5 7.7 6.7 

 1530 7.3 7.7 7.2 7.9 7.6 6.4 7.8 7.5 6.9 6.4 7.8 6.9 7.6 

 1531 6.3 6.8 6.0 6.7 6.3 6.9 6.7 5.5 5.9 6.2 6.5 6.6 5.9 5.8 6.1 6.3 6.3 

 1532 NOT PREGNANT 

 1533 6.2 6.2 6.3 6.4 6.6 6.2 7.1 6.7 5.8 5.4 5.8 6.6 5.5 5.3 6.0 6.1 6.4 

 1534 7.4 7.6 7.6 7.3 7.9 7.5 7.5 7.4 7.4 7.4 7.5 6.8 7.5 7.4 

 1535 NOT PREGNANT 

 1536 5.8 6.5 6.3 5.6 6.4 6.0 5.5 5.8 5.1 5.6 5.8 6.0 6.3 6.3 5.3 4.1 

 1537 7.4 7.6 8.1 7.1 6.9 7.7 7.2 6.9 7.8 7.9 7.2 7.0 7.7 7.5 

 1538 8.0 8.8 8.3 7.7 8.3 8.8 7.9 7.3 8.1 7.4 8.1 8.0 7.5 

 1539 6.9 7.0 7.8 6.6 6.8 7.1 6.4 7.2 6.6 6.9 6.2 6.9 7.5 6.5 

 1540 7.1 7.4 8.0 7.5 7.2 7.5 6.7 7.1 6.7 6.5 7.7 6.2 7.4 6.9 7.2 7.1 

 1541 NOT PREGNANT 

 1542 7.4 7.8 6.6 8.2 7.3 8.4 8.0 7.0 7.7 4.6 7.9 7.5 7.8 7.6 7.8 

 1543 7.2 7.6 7.6 7.2 7.7 8.1 7.0 7.2 7.8 7.6 6.5 6.6 7.0 6.9 6.5 6.3 6.8 

 1544 7.0 7.4 7.5 7.7 7.7 7.6 7.2 7.1 7.0 6.4 6.9 6.9 5.8 7.0 6.9 5.4 

 1545 7.0 7.2 7.6 7.4 7.5 7.0 7.0 7.5 7.0 6.7 7.3 6.7 6.8 6.4 6.3 6.3 

 1546 7.0 6.8 7.5 6.3 7.1 7.9 7.7 7.5 7.5 6.7 6.8 6.9 5.7 6.4 6.7 

 1547 6.9 7.2 6.1 7.7 7.1 6.6 6.1 7.6 7.3 6.9 6.5 7.1 6.5 6.7 7.1 

 1548 NOT PREG., NO MATING DATE 

 1549 6.9 7.0 6.7 7.1 6.7 7.0 7.4 7.3 6.8 6.6 6.7 7.4 6.7 6.9 

 1550 NOT PREG., NO MATING DATE 

 1551 6.5 7.7 6.7 7.1 5.5 7.3 5.9 6.6 6.6 6.3 6.7 6.0 6.2 6.5 6.2 6.6 

MEAN 7.0

S.D. 0.50

N 20

PUP STATUS CODES: S-STILLBORN D-DIED 
23-JUN-2009 19:41

Huntingdon Life Sciences 00-4206 PAGE 384 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 7.5 S D 8.1 7.8 8.2 7.9 7.5 7.0 6.9 6.9 6.9 7.3 7.8 

 2527 NOT PREG., NO MATING DATE 

 2528 7.8 S 7.3 8.2 8.4 7.8 7.6 7.9 7.1 8.0 7.8 7.5 7.9 8.2 7.3 7.7 

 2529 7.9 8.2 7.9 8.1 8.2 7.9 8.0 7.4 7.8 7.6 7.8 7.6 8.1 

 2530 7.3 8.3 7.3 7.1 7.1 6.9 7.4 7.2 7.5 7.4 7.5 7.7 7.2 6.5 6.9 8.2 

 2531 7.2 7.4 7.6 6.9 7.4 7.1 7.1 6.8 8.2 7.7 7.9 6.4 7.1 7.4 6.8 6.9 6.6 

 2532 NOT PREGNANT 

 2533 7.4 7.7 7.8 8.3 7.6 7.7 6.7 7.5 6.3 7.2 7.7 7.4 7.0 7.5 7.3 7.1 

 2534 8.8 9.6 9.3 8.1 8.8 8.3 8.6 

 2535 7.0 7.1 8.0 7.3 6.9 7.7 7.6 7.1 6.9 7.6 6.2 6.6 7.1 5.7 6.8 6.8 

 2536 6.3 S 7.0 6.8 6.9 5.7 6.2 6.4 6.3 6.6 6.8 6.2 6.0 6.0 6.1 6.8 5.2 

 2537 7.3 7.3 7.1 8.0 7.9 7.5 7.0 7.4 6.9 7.5 7.1 6.8 

 2538 NOT PREG., NO MATING DATE 

 2539 6.8 D 6.2 7.6 6.9 7.3 6.7 7.7 7.6 6.0 6.6 6.9 6.8 6.3 6.4 

 2540 7.4 Z 7.5 7.0 7.7 7.9 7.7 7.3 6.9 7.5 7.2 7.2 

 2541 7.5 7.2 7.6 7.3 8.1 8.1 7.9 6.9 7.8 7.4 7.1 7.4 7.3 7.2 

 2542 NOT PREG., NO MATING DATE 

 2543 7.5 D 7.6 7.3 7.3 7.8 7.7 7.0 7.2 7.6 7.0 7.5 7.4 7.7 8.4 

 2544 9.5 9.8 9.6 8.9 9.5 

 2545 7.0 7.3 7.3 7.6 7.3 7.3 6.6 7.3 6.5 7.0 7.0 6.4 6.7 

 2546 7.4 S 7.8 7.4 7.6 7.6 7.8 8.2 7.6 7.1 7.4 7.2 7.3 6.3 

 2547 7.4 7.1 6.9 7.8 7.2 7.0 7.5 7.9 7.5 7.7 7.0 7.3 7.5 8.0 

 2548 7.9 8.3 8.3 7.3 7.6 8.6 8.2 8.1 7.6 7.6 7.2 8.0 

 2549 6.2 6.2 M 

 2550 8.6 8.9 8.5 8.6 9.0 8.4 8.4 8.3 8.4 

 2551 7.5 8.3 8.2 7.9 8.2 M 7.2 6.8 7.5 7.3 7.4 7.2 7.7 7.4 6.8 7.0 

MEAN 7.5

S.D. 0.73

N 22

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING Z-CANNIBALIZED 
23-JUN-2009 19:41

Huntingdon Life Sciences 00-4206 PAGE 385 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREGNANT 

 3527 6.8 7.6 7.7 6.2 6.9 6.8 7.5 6.3 7.5 6.9 6.8 6.1 7.1 7.1 7.2 6.0 6.7 6.1 6.4 

 3528 5.6 D 6.4 6.1 5.6 5.4 5.7 5.6 5.6 5.3 4.9 4.9 5.8 

 3529 6.4 6.1 6.7 7.0 6.6 5.9 6.4 6.4 6.1 6.1 6.7 6.7 6.2 6.5 6.3 

 3530 6.6 6.9 6.3 7.2 7.0 6.7 6.5 6.3 6.1 6.5 6.3 6.6 6.6 6.5 

 3531 7.1 7.2 7.6 7.2 7.1 7.6 7.6 7.4 7.1 6.6 7.3 6.9 7.3 6.5 6.4 7.0 

 3532 7.0 7.6 7.4 7.3 6.9 6.6 6.9 7.1 6.3 7.2 6.9 6.6 7.0 7.0 

 3533 7.3 S 7.1 7.7 6.1 7.4 7.7 8.1 7.6 7.5 7.1 7.2 6.5 7.6 7.4 

 3534 6.4 6.5 6.8 6.8 6.6 6.3 7.0 6.1 6.1 6.6 6.5 6.7 6.2 6.7 5.8 6.6 5.8 

 3535 6.7 6.0 7.3 6.8 7.5 7.0 7.2 6.9 6.5 6.6 5.7 6.1 5.7 7.0 6.4 7.2 

 3536 TOTAL LITTER LOSS 

 3537 5.8 5.9 5.7 6.0 5.1 6.5 5.8 5.9 6.1 6.3 5.8 5.7 5.3 5.3 5.8 5.9 5.1 6.0 

 3538 NOT PREG., NO MATING DATE 

 3539 NOT PREG., NO MATING DATE 

 3540 6.7 6.7 7.5 7.7 6.9 5.8 6.2 6.3 7.1 6.5 6.2 7.0 7.0 6.9 6.7 

 3541 6.7 S 7.5 6.8 6.7 6.4 7.2 5.8 7.4 5.9 6.9 6.5 6.4 6.8 

 3542 7.7 7.8 7.8 7.6 7.9 7.6 7.6 7.8 8.1 7.8 8.3 6.9 7.4 7.7 6.9 

 3543 7.1 7.7 7.5 7.5 6.8 7.2 7.0 6.8 7.2 7.0 6.1 6.9 7.2 7.4 

 3544 7.0 7.6 7.8 7.0 7.4 7.1 7.2 3.9 7.2 6.8 7.6 7.2 7.2 7.6 

 3545 6.5 5.9 6.8 6.3 6.3 6.3 6.3 7.2 7.1 7.3 6.3 6.6 5.7 6.3 6.7 6.8 6.6 

 3546 7.5 7.8 7.6 7.5 7.6 8.1 7.4 7.8 7.0 7.9 7.3 7.7 7.4 7.3 7.6 7.2 

 3547 7.1 S Z 7.6 7.1 7.4 6.9 7.4 6.7 7.4 7.0 7.1 7.0 7.1 6.9 7.1 

 3548 TOTAL LITTER LOSS 

 3549 6.9 7.6 7.6 6.9 7.2 6.6 7.1 6.5 7.3 6.5 6.8 6.6 6.9 6.8 6.4 

 3550 NOT PREG., NO MATING DATE 

 3551 9.0 8.7 9.3 9.6 9.0 8.9 8.5 

MEAN 6.9

S.D. 0.71

N 20

PUP STATUS CODES: S-STILLBORN D-DIED Z-CANNIBALIZED 
23-JUN-2009 19:41

Huntingdon Life Sciences 00-4206 PAGE 386 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 6.6 6.8 7.0 6.6 6.7 6.4 7.0 6.6 6.6 6.9 6.3 7.0 5.9 6.7 6.1 5.9 

 4527 7.4 7.9 7.5 7.7 7.6 7.5 7.7 7.9 7.7 7.2 7.0 6.9 7.0 7.2 7.2 

 4528 6.9 7.8 7.0 6.5 7.7 6.9 7.3 6.8 6.5 6.9 7.1 6.7 7.2 6.6 6.8 6.8 6.1 

 4529 7.6 8.1 7.0 7.8 7.5 8.2 7.9 7.6 7.9 7.5 6.8 6.8 8.1 

 4530 NOT PREGNANT 

 4531 6.6 6.5 7.1 5.9 6.8 6.9 7.0 6.7 6.8 6.9 6.4 5.9 6.1 6.5 

 4532 5.6 D 6.0 6.4 6.0 6.5 4.6 6.4 D 5.3 5.9 D 4.6 D 4.7 D D D 

 4533 7.3 7.7 7.5 7.2 7.2 7.8 7.3 7.2 7.1 7.1 7.8 7.4 7.1 6.3 7.4 7.1 

 4534 6.2 6.3 6.0 6.7 6.3 6.3 6.7 6.4 5.7 6.5 6.6 6.3 6.0 5.7 6.2 5.2 6.7 6.3 

 4535 7.9 8.4 8.5 8.3 8.8 7.1 6.1 8.4 7.8 7.6 

 4536 5.9 5.5 6.5 6.1 6.1 5.9 5.2 6.3 5.4 5.7 5.9 5.9 5.9 6.3 5.5 5.6 

 4537 6.7 6.2 7.3 6.7 6.3 6.7 6.5 6.6 7.5 6.7 6.4 6.9 

 4538 6.7 D 7.3 6.8 7.3 6.7 7.1 7.1 6.5 6.8 6.8 7.0 6.6 5.6 5.7 6.8 6.0 

 4539 NOT PREGNANT 

 4540 7.0 7.8 7.2 7.2 6.6 7.5 6.9 6.8 6.6 6.6 7.5 7.1 6.2 

 4541 NOT PREG., NO MATING DATE 

 4542 6.6 6.8 5.8 7.0 6.9 6.8 6.7 6.5 6.9 6.8 6.4 6.6 6.3 

 4543 6.7 7.2 6.8 6.0 7.0 6.7 6.3 7.2 7.0 6.9 6.0 6.7 7.0 6.4 6.9 6.7 

 4544 6.7 7.3 7.4 6.2 7.8 6.5 5.8 6.4 7.4 7.0 6.1 6.9 6.2 6.5 6.8 5.9 

 4545 8.3 8.0 8.7 8.4 8.2 8.5 8.8 8.5 8.0 8.4 8.7 8.0 8.0 8.0 

 4546 7.5 8.0 7.6 7.7 7.8 7.4 7.5 7.8 7.8 8.2 7.7 6.8 7.3 7.8 6.5 7.5 6.8 

 4547 6.9 7.6 6.9 7.1 6.9 6.4 6.7 6.9 6.6 6.6 7.0 6.9 7.4 7.0 6.5 

 4548 7.0 7.9 6.5 7.1 7.0 7.0 6.3 6.6 6.7 7.1 7.2 7.2 7.7 6.2 7.3 6.9 

 4549 FOUND DEAD 

 4550 7.5 6.7 7.6 7.7 7.8 8.6 7.9 8.5 7.6 7.3 7.8 7.1 7.5 6.9 7.2 6.8 6.6 

 4551 7.7 8.3 8.1 7.8 7.5 7.8 7.1 7.7 6.8 7.8 7.8 7.9 7.4 

MEAN 7.0

S.D. 0.64

N 22

PUP STATUS CODES: D-DIED 
23-JUN-2009 19:44

Huntingdon Life Sciences 00-4206 PAGE 387 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 9.9 S 10.9 9.6 10.1 10.0 9.8 10.8 10.4 10.7 9.8 9.0 10.0 9.5 9.6 9.1 

 1527 DIED POST PARTUM 

 1528 9.3 9.6 9.9 8.6 9.7 8.9 9.3 9.1 10.8 9.4 9.8 9.0 8.7 8.9 8.9 9.4 

 1529 10.3 10.2 10.6 10.9 10.6 9.3 10.4 10.4 10.4 11.0 9.5 9.9 10.2 9.6 11.0 10.0 

 1530 10.9 10.9 10.8 11.6 D 9.5 11.9 11.5 10.8 9.4 11.5 10.3 11.2 

 1531 9.7 10.8 9.8 10.5 9.3 11.2 10.6 8.5 9.1 10.0 10.1 9.7 9.2 8.4 9.1 10.1 8.4 

 1532 NOT PREGNANT 

 1533 8.9 8.8 9.6 8.7 9.3 8.5 10.0 10.0 8.9 8.1 8.1 9.4 8.6 8.4 8.8 8.8 9.2 

 1534 11.2 11.6 11.6 11.2 12.0 11.3 11.5 11.3 11.1 11.2 11.0 9.9 11.4 11.1 

 1535 NOT PREGNANT 

 1536 6.1 6.9 6.7 6.0 7.4 6.3 5.8 6.4 5.6 5.9 5.9 6.1 6.6 7.0 5.1 4.1 

 1537 9.9 10.3 9.9 10.5 9.7 10.7 9.8 9.4 9.9 10.4 9.7 9.5 9.3 9.7 

 1538 11.9 12.5 12.5 11.7 12.0 12.4 11.0 14.6 12.0 10.7 11.4 11.2 10.9 

 1539 9.8 9.3 11.2 9.7 9.2 9.7 8.9 10.6 9.6 10.7 8.6 10.0 10.8 8.8 

 1540 10.8 11.2 11.4 11.7 11.4 10.8 9.9 11.1 9.9 10.0 11.9 8.5 11.2 11.5 10.8 11.3 

 1541 NOT PREGNANT 

 1542 10.7 10.9 8.4 12.2 10.2 12.6 11.1 9.9 10.8 6.3 12.0 11.0 11.0 11.6 11.4 

 1543 10.2 11.1 10.7 10.0 11.3 10.7 10.3 10.2 10.8 10.9 10.0 9.4 10.6 9.3 9.0 9.0 10.2 

 1544 10.4 11.0 11.5 11.7 11.5 11.5 10.4 9.9 10.2 9.8 10.6 9.8 8.7 10.8 10.7 8.0 

 1545 10.0 10.4 11.0 10.6 11.2 9.6 10.4 10.1 10.0 9.9 10.4 9.2 10.0 8.9 8.8 8.8 

 1546 9.3 8.9 9.6 7.9 10.7 10.6 10.2 10.0 9.2 9.1 9.4 9.3 7.6 8.6 9.0 

 1547 9.3 9.0 9.1 9.8 10.1 9.3 8.8 10.1 9.7 9.2 8.1 9.5 9.0 9.1 9.2 

 1548 NOT PREG., NO MATING DATE 

 1549 10.2 10.1 10.0 10.4 9.7 10.3 10.8 10.7 10.1 10.2 10.1 10.6 9.6 10.2 

 1550 NOT PREG., NO MATING DATE 

 1551 9.6 11.8 10.0 10.1 7.6 10.7 8.9 9.8 9.7 9.4 9.4 8.9 9.1 9.9 9.0 9.8 

MEAN 9.9

S.D. 1.15

N 20

PUP STATUS CODES: S-STILLBORN D-DIED 
23-JUN-2009 19:44

Huntingdon Life Sciences 00-4206 PAGE 388 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 11.2 S D 12.1 11.7 12.2 11.7 11.2 10.1 10.3 10.7 10.2 11.1 11.8 

 2527 NOT PREG., NO MATING DATE 

 2528 10.9 S 10.8 11.6 11.7 11.4 10.4 10.7 10.1 10.9 11.1 11.2 11.3 11.2 9.8 10.8 

 2529 11.6 11.5 11.5 12.1 11.7 11.7 11.8 11.1 11.6 11.8 11.2 11.5 12.0 

 2530 10.5 11.7 11.0 10.8 10.6 9.7 11.2 7.1 11.1 11.3 10.7 11.3 10.8 10.4 10.0 10.5 

 2531 9.2 9.9 9.8 8.3 10.0 8.9 9.3 9.3 9.5 9.3 10.5 7.9 9.4 9.4 8.4 8.2 8.7 

 2532 NOT PREGNANT 

 2533 9.7 10.6 9.7 10.9 10.4 10.1 8.6 9.4 8.8 9.9 9.9 9.7 8.9 9.6 10.0 9.2 

 2534 12.6 13.2 13.5 11.6 13.1 11.5 12.8 

 2535 8.7 9.6 9.6 9.0 8.3 9.5 9.2 8.5 9.1 8.2 7.2 7.9 9.1 7.6 8.5 9.2 

 2536 8.9 S 9.6 10.0 9.9 8.1 8.8 D 8.9 9.1 9.9 8.1 7.8 8.1 8.4 9.9 7.6 

 2537 11.0 11.0 9.7 11.5 11.8 10.6 10.8 11.3 10.7 11.5 11.4 10.5 

 2538 NOT PREG., NO MATING DATE 

 2539 10.1 D 9.6 11.1 9.7 10.7 9.5 11.5 10.8 9.1 9.9 10.2 10.5 9.2 9.8 

 2540 11.2 Z 11.2 11.0 11.7 11.8 11.6 10.8 10.5 10.9 11.4 11.1 

 2541 11.7 11.2 12.1 12.0 12.0 12.6 12.0 11.1 12.0 12.1 10.9 11.7 11.2 11.3 

 2542 NOT PREG., NO MATING DATE 

 2543 11.0 D 11.4 10.9 10.8 11.3 11.5 10.6 10.7 10.7 10.3 11.1 10.8 11.5 11.6 

 2544 13.8 14.0 14.2 13.3 13.8 

 2545 10.5 11.1 10.7 11.3 10.7 10.7 9.4 10.7 9.9 10.8 10.6 9.1 10.4 

 2546 11.1 S 11.5 11.2 11.3 11.2 12.2 12.2 10.9 10.6 10.9 10.6 11.0 9.0 

 2547 10.3 10.4 10.4 10.7 10.2 9.4 10.5 10.6 10.3 10.8 9.6 10.0 10.1 10.6 

 2548 11.9 12.3 12.2 11.3 11.7 12.6 11.9 12.2 11.8 11.7 11.7 11.7 

 2549 TOTAL LITTER LOSS 

 2550 14.1 14.1 14.0 13.9 14.7 14.5 13.4 14.0 13.9 

 2551 10.9 12.1 11.1 11.7 11.4 M 10.6 10.3 11.0 10.1 10.3 11.0 11.0 11.7 9.9 10.5 

MEAN 11.0

S.D. 1.39

N 21

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING Z-CANNIBALIZED 
23-JUN-2009 19:44

Huntingdon Life Sciences 00-4206 PAGE 389 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREGNANT 

 3527 10.2 11.9 11.2 9.9 10.7 11.2 9.8 10.3 10.9 10.2 9.6 8.5 10.7 10.2 9.6 9.3 9.4 9.4 10.3 

 3528 8.0 D 8.5 8.4 8.2 D 7.7 8.0 7.8 7.5 M M 7.9 

 3529 8.7 7.8 9.1 9.7 9.4 7.8 9.0 8.3 8.9 8.5 9.6 8.4 8.8 8.9 8.2 

 3530 9.8 10.0 9.3 10.6 10.0 10.0 10.0 9.8 9.6 9.4 9.4 10.0 9.3 9.5 

 3531 9.8 9.7 9.7 9.4 10.4 10.1 10.9 10.4 10.3 9.3 9.7 9.4 9.3 9.5 8.6 9.6 

 3532 10.6 11.1 11.4 11.3 10.5 10.6 10.2 10.6 9.1 10.9 10.9 10.3 10.6 10.8 

 3533 10.3 S 9.9 10.7 8.8 10.6 10.6 11.0 10.3 10.8 9.6 10.6 9.8 10.4 10.4 

 3534 9.5 9.1 9.4 10.0 9.7 9.4 9.5 9.2 9.1 10.4 9.8 D 9.3 10.0 8.3 9.8 9.0 

 3535 9.2 8.5 9.9 9.5 10.3 9.6 9.7 9.6 9.2 9.3 7.3 8.5 8.0 9.5 9.2 9.5 

 3536 TOTAL LITTER LOSS 

 3537 7.9 7.9 7.4 8.5 7.6 8.7 7.3 7.9 7.8 8.5 8.0 8.0 7.5 7.9 8.1 7.7 6.6 8.6 

 3538 NOT PREG., NO MATING DATE 

 3539 NOT PREG., NO MATING DATE 

 3540 9.8 9.6 10.8 10.8 10.1 8.4 8.9 9.8 10.2 9.3 9.3 9.9 10.2 9.7 9.5 

 3541 10.0 S 10.9 9.9 9.9 10.1 10.4 9.3 10.7 9.1 10.5 9.8 9.3 10.1 

 3542 11.0 10.8 11.0 10.7 12.0 10.6 11.1 11.5 11.5 11.8 11.7 10.0 9.9 11.3 10.6 

 3543 9.9 10.7 9.9 10.7 9.8 9.9 9.9 9.6 10.0 10.2 8.5 9.5 9.8 10.5 

 3544 10.2 11.4 11.5 10.6 10.8 10.4 10.2 5.0 10.6 9.9 10.7 10.4 9.8 10.8 

 3545 9.4 8.6 9.8 9.8 D 8.9 9.4 10.2 9.9 10.0 10.0 8.8 8.2 9.0 9.6 9.9 9.6 

 3546 10.9 11.4 11.2 10.7 11.3 12.0 10.3 11.2 9.9 11.4 10.2 11.0 10.4 10.6 10.9 10.3 

 3547 9.5 S Z 9.8 9.3 10.1 9.1 10.4 9.0 9.9 9.2 9.1 9.2 9.4 9.2 9.8 

 3548 TOTAL LITTER LOSS 

 3549 9.9 10.6 10.3 9.8 10.4 9.6 10.2 9.4 10.0 9.4 10.2 9.4 9.5 9.7 9.5 

 3550 NOT PREG., NO MATING DATE 

 3551 15.8 15.2 16.4 16.6 15.8 15.5 15.2 

MEAN 10.0

S.D. 1.58

N 20

PUP STATUS CODES: S-STILLBORN D-DIED Z-CANNIBALIZED M-MISSING 
23-JUN-2009 19:44

Huntingdon Life Sciences 00-4206 PAGE 390 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 8.4 8.2 8.8 8.3 8.0 8.4 9.2 8.2 8.9 9.1 8.4 8.9 8.1 8.5 8.0 7.7 

 4527 10.7 11.5 10.4 10.7 11.4 10.7 10.4 11.4 10.9 D 10.2 11.2 9.6 10.2 10.2 

 4528 8.5 7.5 8.9 7.6 10.1 10.3 9.4 7.7 6.6 7.3 9.0 9.4 7.5 8.7 M 8.4 M 

 4529 10.2 11.2 9.2 11.1 10.9 11.2 9.7 9.3 10.9 9.6 9.3 9.4 10.4 

 4530 NOT PREGNANT 

 4531 9.2 8.9 9.6 8.7 9.2 9.7 9.7 8.8 9.4 9.6 9.2 8.9 8.3 9.5 

 4532 7.9 D 8.5 9.0 8.2 8.7 6.4 9.1 D 7.7 8.4 D 6.5 D 6.1 D D D 

 4533 10.4 11.5 10.7 9.6 9.6 10.8 10.8 10.2 10.4 10.0 10.4 10.7 9.6 9.4 10.6 11.0 

 4534 9.1 8.9 9.4 10.1 9.5 9.5 9.9 9.0 8.0 10.0 9.0 9.3 9.6 8.6 8.3 7.4 9.2 9.2 

 4535 11.7 12.3 13.0 12.0 12.7 11.0 9.3 12.0 11.9 11.5 

 4536 8.1 8.0 8.3 9.2 8.4 7.9 7.3 7.5 8.1 8.4 8.3 D 7.9 8.6 8.0 7.7 

 4537 10.8 9.8 12.2 10.6 9.9 10.5 10.3 10.7 12.3 10.7 10.3 11.1 

 4538 10.1 D 11.5 9.7 11.1 10.3 10.4 10.8 9.7 10.8 10.4 10.4 10.0 8.8 8.5 10.5 9.1 

 4539 NOT PREGNANT 

 4540 10.5 11.6 11.0 11.0 9.6 11.3 10.4 10.4 10.3 9.9 10.8 10.8 9.1 

 4541 NOT PREG., NO MATING DATE 

 4542 9.1 9.0 7.9 10.3 10.4 9.2 8.8 8.6 9.4 9.0 9.0 9.0 8.4 

 4543 10.2 10.7 10.6 9.3 10.8 10.4 10.1 10.6 10.2 10.0 9.6 10.3 10.5 10.2 10.6 9.6 

 4544 9.6 10.5 9.6 9.7 10.7 9.4 8.3 9.7 10.7 10.3 8.7 10.5 9.1 9.5 9.3 8.4 

 4545 10.9 10.7 11.1 11.4 10.6 11.4 11.8 11.2 10.7 10.4 11.2 9.9 10.3 10.6 

 4546 10.1 10.3 10.2 9.6 10.6 9.5 10.4 10.4 10.0 11.3 10.5 9.5 10.1 10.6 9.5 9.8 9.7 

 4547 9.8 9.6 9.9 9.9 9.7 9.5 9.7 10.0 10.5 9.5 9.7 9.8 10.0 10.1 8.9 

 4548 10.3 11.6 9.0 10.0 10.4 10.0 9.8 10.2 9.7 10.4 10.6 11.1 10.9 9.3 11.6 9.9 

 4549 FOUND DEAD 

 4550 10.7 D 10.8 10.7 11.0 11.9 11.4 11.9 11.1 10.8 11.5 10.7 9.7 9.8 10.0 9.9 9.4 

 4551 10.8 11.7 11.7 10.9 10.5 10.6 10.7 10.9 9.7 11.2 10.9 10.7 10.4 

MEAN 9.9

S.D. 1.02

N 22

PUP STATUS CODES: D-DIED M-MISSING 
23-JUN-2009 19:45

Huntingdon Life Sciences 00-4206 PAGE 391 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 13.8 S 14.4 13.6 13.8 14.4 13.6 C C C 13.9 13.0 14.0 13.4 13.4 C 

 1527 DIED POST PARTUM 

 1528 12.8 C 13.3 11.5 13.2 C C 12.0 14.5 C 13.2 12.3 11.9 C 11.9 13.7 

 1529 14.3 14.2 14.4 14.9 C C C 14.2 C 15.2 13.7 14.0 14.2 12.8 15.0 C 

 1530 14.2 14.6 14.2 15.3 D 12.3 15.6 14.7 C 12.5 14.6 13.5 14.7 

 1531 14.1 C 14.3 C 14.4 16.5 15.2 12.6 12.8 14.6 C C 13.4 12.8 C C 14.2 

 1532 NOT PREGNANT 

 1533 13.0 13.1 C 13.2 13.6 C C 14.3 13.3 11.9 12.4 C 12.6 12.8 12.9 C C 

 1534 15.8 15.9 16.3 15.8 16.7 C 15.9 C C 15.2 15.4 14.6 15.5 16.2 

 1535 NOT PREGNANT 

 1536 7.7 C 8.8 7.3 8.9 7.4 6.9 C 6.8 7.9 7.3 7.1 8.5 C C C 

 1537 13.6 C 13.6 14.3 13.2 14.7 14.0 12.7 C C 13.6 13.4 13.4 13.6 

 1538 16.5 17.6 17.2 16.7 16.5 17.4 15.9 14.7 17.1 C 16.1 15.8 C 

 1539 13.9 C C 14.2 13.8 13.6 12.6 15.1 14.3 15.3 12.2 C 14.9 12.7 

 1540 16.4 16.7 17.7 16.9 16.5 15.8 C 16.5 C 14.7 C C C 16.2 15.5 17.1 

 1541 NOT PREGNANT 

 1542 14.0 C 11.7 16.3 14.2 15.9 15.2 13.6 14.1 8.6 16.1 C C 14.6 C 

 1543 14.9 C 15.6 15.0 16.2 C C C 16.2 16.3 14.7 13.9 C C 12.9 13.0 15.0 

 1544 15.6 C 16.5 17.3 17.2 18.2 C C 16.0 15.0 16.3 14.8 13.0 C C 12.1 

 1545 14.2 15.2 15.2 16.2 C C C 14.6 14.1 C 15.0 12.8 13.5 C 12.4 12.6 

 1546 12.5 12.4 12.9 11.5 13.9 C C 13.6 C C 12.6 13.2 10.9 11.5 12.5 

 1547 12.8 12.4 12.1 13.9 C 12.6 12.4 C 13.3 12.4 C C 13.3 12.7 12.8 

 1548 NOT PREG., NO MATING DATE 

 1549 13.6 11.4 13.9 13.7 13.2 13.8 C 14.6 C 13.9 13.7 14.4 C 13.8 

 1550 NOT PREG., NO MATING DATE 

 1551 14.4 17.2 15.0 15.3 11.7 14.5 13.5 C 14.9 14.0 C C 13.7 C C 14.5 

MEAN 13.9

S.D. 1.85

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED 
23-JUN-2009 19:45

Huntingdon Life Sciences 00-4206 PAGE 392 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 14.0 S D 11.5 14.6 15.2 14.6 14.7 C 13.5 13.7 13.2 14.3 14.7 

 2527 NOT PREG., NO MATING DATE 

 2528 14.8 S 14.2 16.2 15.3 C 13.6 15.3 C C 14.3 14.7 C 15.2 13.7 15.4 

 2529 14.6 14.7 14.9 15.0 14.6 14.8 C 14.3 14.4 14.5 14.1 C 15.1 

 2530 15.5 C 17.7 16.1 16.5 15.2 C 7.9 C C 16.9 17.1 16.2 C 15.3 16.4 

 2531 12.7 14.0 C C C C 12.7 12.9 C 13.7 13.9 11.2 12.8 12.4 C 10.8 12.3 

 2532 NOT PREGNANT 

 2533 13.7 14.4 13.6 15.0 14.3 14.3 12.0 C C C C C 12.8 13.6 14.0 13.1 

 2534 15.6 16.3 16.6 14.7 15.8 13.7 16.3 

 2535 11.9 C C 12.6 11.9 C 13.0 C 11.9 11.9 10.2 C 12.5 10.3 11.8 12.5 

 2536 13.7 S 15.4 14.9 14.9 12.7 13.2 D C C 14.6 12.0 12.4 C C 15.0 12.0 

 2537 14.0 14.0 13.1 14.6 14.9 13.8 C 14.1 13.6 14.6 14.3 13.2 

 2538 NOT PREG., NO MATING DATE 

 2539 13.8 D 13.3 C 13.5 15.1 C 15.7 14.5 12.7 13.9 14.1 C 12.2 12.6 

 2540 14.7 Z 15.0 14.4 15.3 14.8 15.6 13.9 13.8 14.6 14.9 14.4 

 2541 16.8 16.1 16.6 17.3 18.2 17.2 C 15.7 17.0 17.3 C C 15.9 16.6 

 2542 NOT PREG., NO MATING DATE 

 2543 15.6 D 15.7 15.4 15.0 16.0 15.4 15.2 C 15.8 14.5 C C 16.8 15.8 

 2544 16.9 18.1 15.8 16.5 17.0 

 2545 13.9 14.9 13.6 15.2 14.2 14.1 C C 12.8 14.6 13.9 12.3 13.9 

 2546 14.8 S 15.6 15.2 C 15.3 16.1 16.8 14.5 13.7 C 14.1 14.1 12.3 

 2547 13.4 13.1 13.6 13.9 C 12.7 13.8 13.6 13.5 13.8 12.8 13.0 C C 

 2548 15.1 14.8 C 14.3 15.3 15.3 14.8 16.0 15.8 14.5 15.0 15.5 

 2549 TOTAL LITTER LOSS 

 2550 18.0 18.7 17.5 17.7 18.5 18.3 17.3 18.0 17.6 

 2551 15.7 16.9 15.8 16.9 16.3 M C C 15.5 14.4 15.3 16.0 15.6 C 14.5 C 

MEAN 14.7

S.D. 1.44

N 21

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING Z-CANNIBALIZED 
23-JUN-2009 19:45

Huntingdon Life Sciences 00-4206 PAGE 393 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREGNANT 

 3527 15.2 17.1 C 15.0 C 16.6 14.9 16.0 C 15.1 14.7 C C C C 13.5 C 14.1 15.3 

 3528 10.4 D 10.9 10.8 10.3 D 10.0 10.1 10.5 9.9 M M 10.3 

 3529 13.0 11.6 13.7 13.4 14.0 C C 12.1 C 12.3 14.2 C 13.3 12.9 12.4 

 3530 14.0 13.8 14.3 C 14.2 14.4 13.8 14.1 C 13.8 13.6 14.0 C 14.1 

 3531 14.0 13.9 14.1 C C 14.1 C 14.3 14.5 C 14.2 14.0 13.5 13.5 C 13.5 

 3532 14.6 15.4 15.1 15.6 14.1 14.6 14.3 C C 14.3 C 13.7 14.3 14.7 

 3533 14.1 S 13.8 14.6 C 14.4 C 14.9 14.6 14.3 13.2 14.1 13.3 C 14.0 

 3534 13.9 14.0 C C 14.2 13.5 14.2 13.8 13.4 14.3 14.4 D C 14.3 12.4 C C 

 3535 12.3 11.4 13.4 C 13.8 12.6 12.4 13.0 11.9 C C 11.0 10.7 12.4 C C 

 3536 TOTAL LITTER LOSS 

 3537 11.6 11.6 C C 11.4 12.5 C C 11.2 12.2 12.0 C 11.1 C 11.6 C 9.6 12.6 

 3538 NOT PREG., NO MATING DATE 

 3539 NOT PREG., NO MATING DATE 

 3540 13.3 13.2 14.5 14.7 14.5 11.5 12.3 C C C 13.2 13.0 13.4 C 12.6 

 3541 15.0 S 16.1 14.5 14.9 15.3 15.1 13.7 15.9 C C 14.7 13.9 15.5 

 3542 15.5 15.3 15.4 C 16.8 15.1 C C C 16.7 16.5 14.3 14.1 15.5 14.9 

 3543 13.3 14.1 13.2 14.3 12.9 13.5 13.2 12.8 13.5 C 11.6 C C 13.6 

 3544 13.3 15.4 15.0 13.7 14.3 13.7 C 5.9 14.0 13.2 14.3 C C 13.6 

 3545 12.6 11.7 13.4 13.1 D C C 14.3 C 13.3 C 11.7 11.3 11.8 12.9 C 12.7 

 3546 14.9 15.3 15.5 15.1 15.0 15.8 C C 13.8 C 14.2 15.3 C 15.0 C 14.2 

 3547 12.6 S Z 12.3 12.1 13.2 C 13.2 11.8 13.6 12.6 12.3 C 13.1 11.4 C 

 3548 TOTAL LITTER LOSS 

 3549 13.5 C C 14.1 14.0 13.1 14.0 13.0 C C 13.5 13.8 12.7 13.5 12.9 

 3550 NOT PREG., NO MATING DATE 

 3551 21.2 20.7 21.7 22.4 21.2 21.0 20.4 

MEAN 13.9

S.D. 2.14

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED M-MISSING 
23-JUN-2009 19:45

Huntingdon Life Sciences 00-4206 PAGE 394 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 11.4 C C C 11.2 11.6 12.4 10.8 11.9 12.1 C 11.6 10.8 C 10.8 10.3 

 4527 14.5 C C 14.7 15.4 C 14.1 15.2 15.7 D 13.5 14.3 13.4 14.2 14.2 

 4528 10.9 9.9 11.4 10.0 14.1 C C M 9.5 10.4 C C 10.9 11.2 M 11.1 M 

 4529 12.1 C C 12.8 12.9 13.3 12.1 11.9 12.6 11.3 10.7 11.6 12.0 

 4530 NOT PREGNANT 

 4531 12.1 11.9 12.6 11.3 C 13.2 12.3 11.7 12.0 12.6 11.9 C C 11.8 

 4532 10.3 D 11.0 11.9 11.0 11.6 8.5 11.4 D 10.5 11.5 D 8.6 D 7.4 D D D 

 4533 14.4 15.4 14.0 14.1 13.8 14.7 15.0 C 14.0 C C C 14.1 13.3 15.6 C 

 4534 12.6 11.8 13.4 14.5 C C 14.3 C 12.0 C C C 12.9 11.9 11.6 10.6 13.1 C 

 4535 14.9 15.8 16.2 14.9 16.0 13.9 12.0 15.2 15.4 14.5 

 4536 11.9 11.5 13.1 12.5 11.9 C 10.0 C 11.6 11.8 11.8 D C 13.0 11.4 C 

 4537 13.5 12.2 15.7 12.8 12.2 13.0 12.6 13.0 15.9 C 12.6 14.8 

 4538 14.3 D 16.0 14.6 15.3 13.8 15.2 C C C C C 14.6 12.5 12.0 15.6 13.8 

 4539 NOT PREGNANT 

 4540 13.5 C 14.1 14.2 12.5 14.4 13.6 13.6 12.8 12.8 13.9 13.1 C 

 4541 NOT PREG., NO MATING DATE 

 4542 12.3 12.0 10.4 14.2 13.4 12.6 11.7 C C 12.7 12.2 12.1 11.4 

 4543 13.7 14.2 14.2 C 13.6 13.8 13.7 14.4 13.6 C 12.4 13.7 13.5 C C C 

 4544 14.1 14.9 C 14.4 14.8 13.8 C C 15.2 14.9 12.6 14.4 C C 13.6 12.4 

 4545 14.1 13.6 14.7 15.1 C 14.9 C C 14.3 13.4 14.8 12.9 13.5 13.6 

 4546 13.7 14.2 C C 14.2 C C 14.0 13.5 14.8 C 13.0 13.7 C 12.9 13.6 13.1 

 4547 12.6 12.5 12.8 12.8 12.4 12.3 C 12.8 C C C 12.7 12.9 12.9 11.4 

 4548 14.8 16.6 12.7 14.8 14.9 14.9 C C 14.3 14.8 15.2 15.7 C 13.6 C C 

 4549 FOUND DEAD 

 4550 14.3 D C 13.8 15.2 15.3 C 15.3 15.0 13.9 15.0 C 13.1 C 13.4 12.9 C 

 4551 13.4 14.6 14.5 13.9 13.1 13.4 13.5 C 11.6 C 13.2 13.3 12.8 

MEAN 13.1

S.D. 1.30

N 22

PUP STATUS CODES: D-DIED C-CULLED M-MISSING 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 395 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 24.6 S 25.6 24.1 25.6 25.2 24.4 C C C 24.3 23.8 24.1 24.5 24.3 C 

 1527 DIED POST PARTUM 

 1528 23.7 C 23.1 21.6 25.4 C C 23.9 24.3 C 24.9 24.2 21.9 C 22.3 25.9 

 1529 27.2 29.1 27.0 28.0 C C C 26.3 C 28.0 25.7 26.2 28.2 25.2 28.4 C 

 1530 24.9 25.0 24.7 24.4 D 22.1 27.2 26.0 C 23.4 26.2 24.9 25.5 

 1531 25.4 C 25.1 C 25.2 28.7 26.5 23.5 22.6 26.9 C C 25.1 23.8 C C 26.2 

 1532 NOT PREGNANT 

 1533 24.0 24.6 C 24.4 23.2 C C 25.9 24.8 23.9 23.3 C 22.4 23.7 23.9 C C 

 1534 26.0 26.6 26.5 25.8 27.6 C 26.7 C C 25.5 25.8 23.7 25.0 26.3 

 1535 NOT PREGNANT 

 1536 16.1 C 17.8 15.5 20.9 16.2 12.7 C 12.9 16.9 15.3 15.3 17.8 C C C 

 1537 24.4 C 25.8 24.3 25.9 25.0 22.5 25.1 C C 23.6 23.7 24.5 23.3 

 1538 27.0 28.6 27.4 27.8 27.7 27.2 26.8 25.0 27.2 C 26.7 25.4 C 

 1539 25.9 C C 24.9 24.0 24.7 23.7 30.0 25.4 29.5 23.3 C 28.8 24.3 

 1540 28.5 28.7 31.4 28.5 28.7 27.6 C 29.5 C 25.8 C C C 27.7 27.5 29.4 

 1541 NOT PREGNANT 

 1542 22.6 C 20.6 26.2 23.5 24.2 25.8 22.3 22.8 13.0 25.1 C C 22.4 C 

 1543 28.8 C 29.7 29.9 30.0 C C C 31.6 30.2 28.6 27.6 C C 25.9 25.9 28.6 

 1544 31.7 C 31.2 32.5 32.2 36.1 C C 31.9 31.4 32.8 30.5 26.7 C C D 

 1545 26.9 28.5 27.0 29.9 C C C 27.1 26.3 C 28.3 25.4 26.7 C 25.3 24.9 

 1546 22.3 22.1 23.0 21.0 24.1 C C 23.5 C C 21.6 23.7 20.7 21.2 22.0 

 1547 22.5 22.1 22.1 24.4 C 22.7 22.2 C 23.1 22.0 C C 22.8 21.6 21.9 

 1548 NOT PREG., NO MATING DATE 

 1549 23.1 19.3 22.5 22.3 22.4 24.3 C 24.9 C 23.1 24.0 25.0 C 22.9 

 1550 NOT PREG., NO MATING DATE 

 1551 25.6 28.1 26.1 27.0 23.2 25.6 25.5 C 26.1 24.8 C C 24.4 C C 24.9 

MEAN 25.1

S.D. 3.17

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 396 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 25.5 S D M 26.2 27.2 25.3 26.4 C 24.0 24.9 24.2 25.9 25.5 

 2527 NOT PREG., NO MATING DATE 

 2528 25.5 S 25.1 28.2 25.8 C 24.6 26.2 C C 24.1 25.7 C 25.8 24.0 25.8 

 2529 23.3 24.0 23.7 23.5 22.4 23.2 C 22.7 22.5 23.2 23.0 C 24.9 

 2530 29.9 C 33.1 30.1 31.3 27.5 C M C C 26.2 28.7 30.3 C 31.8 30.3 

 2531 22.4 24.3 C C C C 23.5 23.1 C 23.3 24.1 18.4 21.9 22.5 C 19.8 22.6 

 2532 NOT PREGNANT 

 2533 24.1 24.3 23.7 24.6 25.3 24.6 22.1 C C C C C 23.8 23.2 25.2 23.9 

 2534 22.4 23.4 22.7 21.6 23.2 20.5 22.7 

 2535 18.3 C C 19.4 18.0 C 20.5 C 18.3 19.1 15.8 C 19.0 16.1 18.2 18.3 

 2536 25.3 S 28.4 25.7 27.1 24.5 25.2 D C C 25.4 24.0 23.3 C C 26.8 23.0 

 2537 22.1 21.4 21.4 23.1 23.6 22.0 C 21.7 21.6 22.1 22.0 21.6 

 2538 NOT PREG., NO MATING DATE 

 2539 24.7 D 23.9 C 23.3 25.0 C 28.3 25.1 24.0 25.6 25.9 C 22.3 23.1 

 2540 24.4 Z 24.6 25.0 24.5 24.8 25.4 23.8 22.2 24.4 24.9 24.3 

 2541 28.5 27.6 28.7 29.4 29.4 29.7 C 27.0 28.3 29.2 C C 28.1 28.1 

 2542 NOT PREG., NO MATING DATE 

 2543 23.8 D 23.3 22.9 22.5 24.3 21.3 24.5 C 25.4 23.3 C C 25.1 25.0 

 2544 25.7 27.0 24.0 24.9 26.8 

 2545 23.1 23.7 22.6 24.5 24.2 23.4 C C 20.7 23.2 23.5 21.8 23.2 

 2546 25.1 S 25.9 26.0 C 27.1 26.5 26.7 24.4 23.5 C 23.8 24.0 23.3 

 2547 21.4 21.5 21.2 22.1 C 21.3 22.0 21.2 21.1 21.5 20.6 21.8 C C 

 2548 25.7 25.9 C 26.4 24.0 26.3 24.7 28.0 27.0 25.2 25.1 24.4 

 2549 TOTAL LITTER LOSS 

 2550 28.5 30.7 27.0 27.3 29.2 29.1 27.8 29.5 27.4 

 2551 26.5 29.2 26.0 27.3 27.2 M C C 26.4 25.2 26.4 26.7 26.4 C 24.4 C 

MEAN 24.6

S.D. 2.64

N 21

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING Z-CANNIBALIZED 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 397 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREGNANT 

 3527 25.4 27.8 C 25.9 C 29.2 26.1 17.5 C 25.9 25.6 C C C C 23.9 C 25.3 26.3 

 3528 19.6 D 19.6 18.6 20.5 D 20.0 20.3 20.7 18.2 M M 18.9 

 3529 22.8 21.3 23.3 23.0 22.9 C C 22.0 C 21.0 24.7 C 23.9 22.0 24.4 

 3530 24.3 24.4 23.4 C 24.7 25.3 25.0 24.2 C 25.2 23.5 23.1 C 24.3 

 3531 24.5 23.6 24.9 C C 23.8 C 25.1 25.1 C 25.0 24.3 24.9 24.0 C 23.8 

 3532 24.4 25.9 20.6 26.2 24.4 25.7 24.8 C C 24.3 C 23.8 23.7 24.8 

 3533 26.3 S 25.2 26.9 C 27.9 C 25.9 27.3 26.1 25.3 26.4 26.0 C 26.2 

 3534 24.3 25.1 C C 24.9 23.9 25.0 24.5 23.2 25.0 24.6 D C 24.2 22.1 C C 

 3535 22.2 21.9 24.4 C 25.5 23.1 21.2 23.4 21.4 C C 19.1 20.5 21.6 C C 

 3536 TOTAL LITTER LOSS 

 3537 20.7 20.6 C C 20.1 22.3 C C 19.1 21.6 21.7 C 20.5 C 20.6 C 18.5 21.7 

 3538 NOT PREG., NO MATING DATE 

 3539 NOT PREG., NO MATING DATE 

 3540 22.1 21.5 24.6 23.4 23.0 19.0 21.4 C C C 22.0 22.9 21.8 C 21.4 

 3541 24.9 S 26.8 24.0 24.6 25.0 25.4 22.8 25.8 C C 25.1 24.0 25.2 

 3542 26.7 26.6 26.3 C 29.0 26.4 C C C 28.2 27.5 26.4 24.6 26.4 25.4 

 3543 22.7 23.0 22.8 23.3 21.5 23.7 23.0 22.7 22.5 C 21.0 C C 23.7 

 3544 23.3 26.1 26.3 24.8 25.2 23.4 C 10.7 23.9 23.9 25.1 C C 23.2 

 3545 23.6 22.3 23.2 26.6 D C C 26.4 C 24.2 C 22.3 22.5 20.6 22.2 C 25.4 

 3546 26.4 27.6 26.3 26.0 26.1 28.4 C C 25.0 C 25.2 27.1 C 26.4 C 25.7 

 3547 24.1 S Z 24.3 22.7 24.7 C 23.7 23.6 25.1 24.0 23.7 C 25.6 23.7 C 

 3548 TOTAL LITTER LOSS 

 3549 24.5 C C 25.2 25.2 24.2 24.9 23.2 C C 24.9 24.7 23.4 25.5 23.4 

 3550 NOT PREG., NO MATING DATE 

 3551 33.7 33.6 34.0 34.5 33.0 34.1 33.1 

MEAN 24.3

S.D. 2.86

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED M-MISSING 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 398 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 19.2 C C C 19.7 19.1 21.1 18.0 19.9 19.7 C 19.9 18.5 C 18.9 17.1 

 4527 23.2 C C 24.1 23.6 C 22.8 24.2 25.6 D 22.1 23.2 21.6 22.6 22.5 

 4528 18.8 17.5 20.0 17.6 22.6 C C M 15.0 17.2 C C 19.1 19.9 M 20.1 M 

 4529 20.4 C C 22.2 21.1 22.3 19.4 19.8 21.9 19.2 18.0 19.2 20.7 

 4530 NOT PREGNANT 

 4531 19.5 19.6 19.4 18.7 C 20.7 19.8 19.9 19.1 19.3 19.7 C C 18.9 

 4532 21.0 D 22.3 22.5 21.4 23.8 17.9 21.8 D 21.9 23.3 D 19.3 D 16.0 D D D 

 4533 25.5 26.5 24.8 26.3 23.8 24.5 27.9 C 25.2 C C C 26.0 23.1 27.4 C 

 4534 24.0 23.3 25.6 25.9 C C 27.2 C 24.1 C C C 23.5 22.0 22.6 21.8 24.4 C 

 4535 22.7 24.8 24.9 22.3 23.0 20.8 19.0 24.1 22.3 22.7 

 4536 22.8 22.6 23.5 23.9 22.5 C 20.7 C 22.8 22.2 22.9 D C 24.4 22.8 C 

 4537 25.6 23.7 29.0 23.9 24.0 24.3 23.4 24.8 30.7 C 23.4 28.8 

 4538 24.3 D 26.0 24.5 25.2 22.4 25.2 C C C C C 25.3 22.6 21.4 25.7 24.4 

 4539 NOT PREGNANT 

 4540 20.6 C 20.7 21.3 20.3 21.7 20.4 20.4 20.2 19.9 21.1 19.7 C 

 4541 NOT PREG., NO MATING DATE 

 4542 22.3 23.4 19.6 23.5 24.2 22.7 22.8 C C 22.6 22.0 21.5 21.0 

 4543 23.2 22.7 24.9 C 24.2 22.2 23.1 24.8 22.7 C 21.9 23.3 22.4 C C C 

 4544 23.5 24.5 C 23.8 23.8 22.9 C C 24.5 25.0 22.0 23.1 C C 23.0 22.4 

 4545 24.2 23.9 24.9 25.1 C 26.5 C C 23.6 23.4 24.4 22.2 24.0 23.7 

 4546 24.4 24.0 C C 24.8 C C 25.8 23.4 25.5 C 24.2 24.7 C 23.3 23.5 25.0 

 4547 22.1 23.3 22.6 21.8 21.6 22.9 C 19.8 C C C 20.4 23.2 23.3 22.4 

 4548 26.0 28.2 23.9 25.8 26.5 27.0 C C 24.0 25.9 27.3 26.1 C 25.7 C C 

 4549 FOUND DEAD 

 4550 24.1 D C 24.0 25.3 25.7 C 24.6 25.0 23.6 25.9 C 22.7 C 22.2 21.7 C 

 4551 22.1 22.2 22.9 23.0 23.1 21.9 22.4 C 20.7 C 22.3 21.4 21.6 

MEAN 22.7

S.D. 2.09

N 22

PUP STATUS CODES: D-DIED C-CULLED M-MISSING 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 399 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 41.0 S 43.3 36.6 43.6 43.0 40.8 C C C 40.4 41.3 40.4 40.1 40.5 C 

 1527 DIED POST PARTUM 

 1528 35.0 C 32.8 33.1 39.0 C C 34.9 35.5 C 37.3 33.3 31.7 C 33.5 39.2 

 1529 42.7 44.4 43.0 46.9 C C C 41.0 C 42.2 39.1 42.7 45.1 36.6 45.5 C 

 1530 38.4 37.4 40.4 36.7 D 33.7 43.5 40.8 C 35.7 38.2 38.1 39.4 

 1531 40.0 C 37.0 C 39.8 43.0 41.5 38.8 37.2 42.7 C C 41.7 35.7 C C 42.9 

 1532 NOT PREGNANT 

 1533 38.6 37.1 C 38.3 37.6 C C 43.3 39.5 37.9 36.9 C 35.2 40.0 39.9 C C 

 1534 43.9 48.3 45.3 44.2 47.3 C 42.4 C C 43.3 39.1 42.4 41.7 44.6 

 1535 NOT PREGNANT 

 1536 25.2 C 26.8 24.0 30.5 24.3 19.4 C 22.0 27.0 23.9 25.9 28.5 C C C 

 1537 39.2 C 42.5 36.6 37.6 41.5 42.5 33.3 C C 42.2 38.8 39.1 38.2 

 1538 41.9 44.2 38.0 44.2 43.8 41.9 41.9 38.8 43.5 C 42.0 40.6 C 

 1539 40.1 C C 37.3 38.0 39.0 35.4 41.4 45.9 39.2 46.0 C 39.1 39.6 

 1540 46.2 49.3 52.4 49.1 45.9 39.9 C 49.3 C 41.3 C C C 47.2 43.0 44.5 

 1541 NOT PREGNANT 

 1542 36.4 C 32.6 43.2 38.8 37.4 42.3 36.4 36.3 17.5 42.9 C C 36.4 C 

 1543 49.5 C 49.7 52.6 51.6 C C C 53.5 52.1 50.9 48.1 C C 45.2 44.0 47.6 

 1544 48.8 C 48.8 52.2 51.4 54.4 C C 49.4 48.4 48.9 46.3 39.8 C C D 

 1545 44.5 48.4 45.3 48.2 C C C 48.7 41.5 C 45.4 42.6 43.2 C 40.4 40.8 

 1546 36.4 34.8 37.6 32.4 40.1 C C 39.8 C C 36.8 39.7 32.7 34.2 36.0 

 1547 38.7 37.7 38.4 42.9 C 38.7 39.7 C 36.6 36.0 C C 41.2 37.8 38.0 

 1548 NOT PREG., NO MATING DATE 

 1549 37.2 29.1 34.1 34.4 35.4 39.0 C 42.3 C 38.7 40.8 41.8 C 36.1 

 1550 NOT PREG., NO MATING DATE 

 1551 40.5 41.3 46.0 43.7 37.1 40.8 42.7 C 43.2 37.1 C C 39.3 C C 33.3 

MEAN 40.2

S.D. 5.32

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 400 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 41.4 S D M 41.0 42.2 44.5 44.5 C 37.7 39.9 39.5 41.8 41.3 

 2527 NOT PREG., NO MATING DATE 

 2528 41.1 S 41.9 43.7 42.8 C 41.4 39.8 C C 36.2 39.6 C 42.0 39.5 43.7 

 2529 38.5 38.4 37.9 38.8 36.3 38.3 C 39.1 37.0 37.9 38.6 C 42.4 

 2530 48.7 C 53.6 49.2 43.1 45.0 C M C C 52.5 46.2 47.8 C 49.3 51.6 

 2531 38.3 41.5 C C C C 39.7 39.6 C 38.7 43.3 32.1 36.1 40.2 C 33.9 38.2 

 2532 NOT PREGNANT 

 2533 40.3 40.5 38.4 41.9 39.5 41.4 39.3 C C C C C 39.9 39.4 41.9 40.6 

 2534 37.6 36.8 37.8 36.2 40.1 34.0 40.8 

 2535 29.0 C C 31.1 27.8 C 32.0 C 27.0 29.8 26.8 C 30.5 25.6 29.0 30.8 

 2536 39.8 S 42.8 40.2 44.6 37.5 39.6 D C C 42.5 37.4 35.3 C C 40.4 37.4 

 2537 39.2 38.0 35.6 40.4 40.6 38.7 C 38.6 40.9 39.3 39.9 39.8 

 2538 NOT PREG., NO MATING DATE 

 2539 41.0 D 37.4 C 39.0 43.7 C 46.3 44.2 40.2 43.5 41.9 C 35.9 38.3 

 2540 41.0 Z 43.5 41.4 40.7 42.4 43.5 38.9 35.8 42.2 41.1 40.0 

 2541 46.4 43.2 49.0 42.2 49.8 52.0 C 45.0 44.2 48.2 C C 44.7 46.0 

 2542 NOT PREG., NO MATING DATE 

 2543 40.3 D 40.0 37.2 40.3 40.7 35.6 41.9 C 42.3 39.4 C C 42.0 43.3 

 2544 44.1 47.3 42.2 40.6 46.4 

 2545 39.5 41.8 36.7 41.8 40.9 41.1 C C 35.7 42.6 40.4 36.6 37.2 

 2546 42.7 S 42.7 42.3 C 45.9 46.1 45.4 40.8 42.2 C 41.4 40.7 39.5 

 2547 39.0 38.8 38.9 40.5 C 38.8 40.6 38.6 39.4 40.9 36.5 37.5 C C 

 2548 43.3 44.3 C 44.1 40.1 46.2 41.9 47.1 44.1 41.3 43.4 40.8 

 2549 TOTAL LITTER LOSS 

 2550 49.6 51.2 48.0 50.7 51.3 50.0 48.2 50.5 46.9 

 2551 46.2 50.9 46.1 48.0 47.3 M C C 44.6 45.4 46.0 46.3 46.1 C 41.4 C 

MEAN 41.3

S.D. 4.40

N 21

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING Z-CANNIBALIZED 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 401 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREGNANT 

 3527 45.6 48.6 C 45.9 C 49.3 45.8 46.5 C 44.7 43.8 C C C C 41.3 C 43.7 46.7 

 3528 31.7 D 30.0 29.0 34.3 D 32.9 33.0 34.3 29.8 M M 29.9 

 3529 36.2 33.2 36.6 35.6 37.7 C C 34.8 C 33.1 39.8 C 39.1 35.7 36.0 

 3530 40.2 43.5 41.0 C 35.8 38.9 41.0 42.7 C 41.1 39.6 36.9 C 41.5 

 3531 42.3 42.0 42.1 C C 39.7 C 42.6 43.0 C 46.9 41.0 43.1 42.4 C 39.8 

 3532 39.5 39.5 33.2 42.5 40.1 41.1 39.4 C C 39.5 C 39.9 37.2 42.4 

 3533 45.9 S 43.8 46.8 C 48.0 C 44.3 46.9 46.7 45.1 47.0 45.7 C 45.0 

 3534 42.4 40.6 C C 44.4 43.5 44.8 41.3 40.8 44.5 42.3 D C 44.1 38.0 C C 

 3535 37.2 36.2 36.7 C 43.2 39.9 36.7 39.6 37.5 C C 33.1 32.1 36.8 C C 

 3536 TOTAL LITTER LOSS 

 3537 33.2 29.9 C C 33.2 33.8 C C 32.9 36.7 34.2 C 32.9 C 32.7 C 28.7 36.6 

 3538 NOT PREG., NO MATING DATE 

 3539 NOT PREG., NO MATING DATE 

 3540 33.9 31.6 36.9 35.6 35.2 29.5 31.5 C C C 35.0 37.4 32.2 C 33.8 

 3541 41.1 S 43.1 38.2 39.6 42.8 42.9 38.7 42.3 C C 41.0 40.1 42.2 

 3542 47.5 47.5 47.9 C 50.5 48.0 C C C 48.7 48.1 45.7 43.1 49.0 46.5 

 3543 36.5 36.6 31.6 39.4 37.6 38.3 34.4 37.0 36.8 C 33.8 C C 39.0 

 3544 37.2 41.1 40.5 41.7 39.3 38.0 C 16.8 39.4 37.0 39.5 C C 38.3 

 3545 38.4 37.9 36.0 45.3 D C C 41.7 C 38.8 C 34.4 39.6 33.6 35.4 C 41.6 

 3546 44.4 45.6 45.5 44.5 42.4 47.5 C C 41.0 C 44.0 44.6 C 46.5 C 42.1 

 3547 39.7 S Z 40.6 36.9 41.1 C 39.0 38.2 38.9 41.5 39.7 C 43.4 37.8 C 

 3548 TOTAL LITTER LOSS 

 3549 41.3 C C 42.0 43.0 41.1 43.3 39.5 C C 41.6 40.4 39.5 42.9 40.1 

 3550 NOT PREG., NO MATING DATE 

 3551 58.5 59.2 62.6 61.0 53.1 57.8 57.3 

MEAN 40.6

S.D. 6.03

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED M-MISSING 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 402 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 32.3 C C C 34.9 32.6 34.9 29.1 33.1 35.3 C 33.2 30.5 C 31.1 28.6 

 4527 37.4 C C 39.2 39.0 C 35.0 38.2 40.3 D 36.0 35.4 36.3 38.9 35.6 

 4528 32.0 30.0 34.2 29.6 38.4 C C M 28.3 30.1 C C 32.1 32.5 M 32.6 M 

 4529 31.8 C C 35.4 32.6 36.8 28.5 29.8 32.7 30.1 27.7 30.6 33.4 

 4530 NOT PREGNANT 

 4531 33.0 32.5 32.1 30.9 C 34.0 33.0 35.2 33.9 33.2 33.6 C C 32.0 

 4532 35.0 D 37.5 37.6 34.6 39.8 30.0 35.1 D 36.2 40.3 D 32.9 D 25.7 D D D 

 4533 43.9 45.5 44.3 43.1 40.1 43.7 47.4 C 42.5 C C C 44.2 41.9 46.0 C 

 4534 38.1 37.7 40.4 39.1 C C 42.3 C 38.0 C C C 39.1 34.2 34.4 35.1 40.3 C 

 4535 37.2 43.0 39.9 36.0 35.0 33.1 29.7 42.6 36.4 39.0 

 4536 38.5 37.6 41.2 39.3 38.1 C 33.7 C 39.1 38.3 38.3 D C 41.1 37.8 C 

 4537 42.9 38.4 49.8 40.0 40.5 40.3 40.0 40.3 51.5 C 39.6 48.5 

 4538 39.1 D 42.3 38.5 39.6 37.6 41.3 C C C C C 41.8 35.3 33.8 41.8 38.7 

 4539 NOT PREGNANT 

 4540 33.4 C 31.7 32.9 33.2 35.6 33.8 34.2 31.3 31.4 35.9 33.9 C 

 4541 NOT PREG., NO MATING DATE 

 4542 37.5 38.3 31.6 40.1 41.0 37.2 36.8 C C 38.2 37.3 36.7 37.8 

 4543 39.7 40.1 43.0 C 40.1 36.8 39.1 43.5 36.7 C 38.3 38.9 40.8 C C C 

 4544 41.4 44.4 C 42.3 39.2 42.1 C C 43.6 42.7 38.3 41.6 C C 42.1 38.1 

 4545 39.8 40.1 38.6 40.2 C 42.8 C C 36.5 38.6 42.5 37.5 38.6 43.0 

 4546 41.9 42.5 C C 42.8 C C 44.4 40.3 43.0 C 40.5 42.3 C 40.0 40.5 42.6 

 4547 36.6 38.4 36.3 36.5 35.3 37.2 C 33.4 C C C 35.5 40.2 36.7 36.8 

 4548 43.7 46.2 38.5 43.6 44.1 45.2 C C 42.4 42.0 46.9 44.4 C 43.3 C C 

 4549 FOUND DEAD 

 4550 42.0 D C 43.1 45.0 43.1 C 41.9 42.4 40.8 46.8 C 40.0 C 39.7 37.3 C 

 4551 35.9 35.9 35.9 32.8 36.4 37.4 40.3 C 32.0 C 36.0 38.4 33.9 

MEAN 37.9

S.D. 3.85

N 22

PUP STATUS CODES: D-DIED C-CULLED M-MISSING 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 403 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 74.8 S 81.1 67.6 81.2 80.5 77.8 C C C 73.9 70.7 73.3 71.3 70.6 C 

 1527 DIED POST PARTUM 

 1528 72.7 C 73.5 68.1 79.6 C C 75.3 76.9 C 73.8 70.5 66.4 C 69.3 73.2 

 1529 82.0 84.3 82.3 91.9 C C C 84.8 C 85.0 71.7 78.2 80.8 75.1 86.3 C 

 1530 72.3 73.2 76.2 71.1 D 64.9 81.4 75.5 C 67.6 72.4 69.7 71.3 

 1531 75.9 C 77.3 C 81.7 83.5 80.5 70.5 67.5 78.6 C C 75.3 67.8 C C 75.9 

 1532 NOT PREGNANT 

 1533 75.7 78.9 C 80.8 77.5 C C 85.3 76.5 70.3 69.7 C 69.0 76.6 72.8 C C 

 1534 78.2 84.9 80.8 80.9 85.6 C 79.3 C C 74.8 72.6 71.5 74.0 78.0 

 1535 NOT PREGNANT 

 1536 49.5 C 52.8 48.7 58.9 51.9 38.9 C 42.8 53.9 44.9 50.2 52.3 C C C 

 1537 72.1 C 73.6 71.3 72.2 75.6 75.0 65.5 C C 79.1 69.4 70.1 69.1 

 1538 80.2 87.7 84.6 85.1 86.6 81.1 77.5 72.8 77.8 C 74.3 74.4 C 

 1539 74.7 C C 70.8 72.0 73.1 71.0 85.6 71.8 78.8 69.9 C 84.5 69.5 

 1540 87.4 94.1 98.9 95.2 91.4 78.5 C 89.7 C 81.9 C C C 82.5 81.9 80.2 

 1541 NOT PREGNANT 

 1542 71.3 C 63.8 83.9 79.0 72.9 81.6 69.6 71.0 38.3 82.2 C C 70.6 C 

 1543 89.5 C 96.8 95.1 100.3 C C C 98.0 97.6 85.6 83.4 C C 76.1 77.9 84.2 

 1544 87.6 C 86.2 95.8 93.6 104.5 C C 93.7 81.4 82.4 81.4 69.6 C C D 

 1545 81.5 87.6 81.6 92.4 C C C 87.0 78.6 C 83.4 77.2 79.0 C 70.5 77.6 

 1546 72.1 70.7 74.2 70.2 79.4 C C 75.5 C C 70.5 77.3 65.6 66.6 71.3 

 1547 75.2 72.5 76.9 84.6 C 75.8 78.1 C 76.4 72.3 C C 71.0 73.7 70.9 

 1548 NOT PREG., NO MATING DATE 

 1549 73.4 63.9 69.1 72.9 74.0 75.2 C 84.1 C 74.3 73.8 75.0 C 72.0 

 1550 NOT PREG., NO MATING DATE 

 1551 79.8 88.0 89.4 81.4 76.5 87.5 80.0 C 82.9 72.5 C C 66.1 C C 73.7 

MEAN 76.3

S.D. 8.39

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 404 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 77.1 S D M 79.0 80.2 85.7 80.6 C 71.7 70.9 73.2 74.8 77.9 

 2527 NOT PREG., NO MATING DATE 

 2528 79.7 S 82.3 86.3 83.5 C 84.2 84.6 C C 75.3 69.9 C 77.8 74.3 78.5 

 2529 73.5 76.5 74.3 72.4 72.5 72.6 C 78.9 67.1 69.1 74.8 C 77.1 

 2530 89.2 C 102.3 91.4 91.3 82.9 C M C C 89.0 89.0 84.4 C 79.6 92.6 

 2531 73.2 78.0 C C C C 78.7 78.9 C 73.3 82.6 65.2 70.5 71.4 C 62.0 71.3 

 2532 NOT PREGNANT 

 2533 75.2 79.5 74.4 77.3 75.6 74.9 71.2 C C C C C 68.7 74.0 81.2 74.8 

 2534 70.6 76.2 72.6 64.2 72.7 63.2 74.9 

 2535 62.5 C C 65.9 62.4 C 66.0 C 60.2 71.2 59.1 C 62.2 54.7 61.4 62.3 

 2536 79.5 S 88.5 83.0 92.3 78.7 74.7 D C C 82.7 71.5 69.8 C C 78.2 75.5 

 2537 73.5 75.7 71.3 78.1 76.3 75.3 C 70.7 75.4 70.6 72.5 69.0 

 2538 NOT PREG., NO MATING DATE 

 2539 76.4 D 73.0 C 76.0 85.5 C 87.8 81.2 73.8 73.4 79.0 C 64.7 69.8 

 2540 78.7 Z 82.9 81.2 80.4 81.8 86.9 76.9 68.8 75.2 77.4 75.5 

 2541 85.6 86.4 91.8 84.8 88.8 93.2 C 80.6 81.1 84.5 C C 82.2 82.4 

 2542 NOT PREG., NO MATING DATE 

 2543 72.6 D 80.2 69.5 72.4 71.7 64.1 75.1 C 74.6 69.1 C C 71.3 77.7 

 2544 83.1 91.1 83.1 76.0 82.1 

 2545 74.7 79.5 73.7 83.4 77.2 78.1 C C 68.8 73.5 73.3 68.9 70.3 

 2546 79.7 S 79.3 84.9 C 85.5 89.3 86.6 75.4 78.0 C 74.0 75.9 67.7 

 2547 75.0 76.8 75.4 77.8 C 78.7 75.9 70.3 78.3 78.0 67.7 71.5 C C 

 2548 79.0 83.1 C 83.3 77.8 87.3 77.5 80.5 78.5 73.0 75.3 73.5 

 2549 TOTAL LITTER LOSS 

 2550 87.2 94.3 85.9 92.4 92.4 86.2 80.9 82.6 82.9 

 2551 84.1 96.3 86.3 87.2 88.8 M C C 80.0 77.6 86.4 81.9 82.6 C 74.0 C 

MEAN 77.6

S.D. 6.14

N 21

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING Z-CANNIBALIZED 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 405 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREGNANT 

 3527 82.8 92.5 C 89.4 C 94.0 83.4 85.9 C 75.9 74.7 C C C C 72.4 C 78.4 81.1 

 3528 66.0 D 68.1 64.0 71.6 D 65.0 68.8 69.1 59.4 M M 61.8 

 3529 73.7 69.7 77.7 78.1 78.0 C C 70.5 C 67.5 80.8 C 76.9 70.8 67.1 

 3530 75.1 81.5 79.1 C 69.7 76.8 77.4 74.7 C 71.9 74.3 69.4 C 76.4 

 3531 77.4 76.1 77.6 C C 77.2 C 77.4 82.2 C 84.9 74.3 78.0 75.5 C 70.7 

 3532 75.5 80.2 67.1 83.9 76.2 74.6 78.0 C C 74.6 C 72.4 68.9 78.7 

 3533 86.5 S 86.7 89.5 C 91.8 C 83.1 89.5 85.8 81.2 86.3 86.8 C 84.4 

 3534 77.3 76.7 C C 82.4 79.4 82.3 77.5 76.5 75.3 75.7 D C 81.7 65.8 C C 

 3535 70.1 73.3 75.8 C 75.8 72.1 69.9 69.3 70.0 C C 61.1 63.3 70.5 C C 

 3536 TOTAL LITTER LOSS 

 3537 65.5 60.7 C C 65.6 64.3 C C 64.2 71.3 70.7 C 63.3 C 66.0 C 58.1 70.7 

 3538 NOT PREG., NO MATING DATE 

 3539 NOT PREG., NO MATING DATE 

 3540 63.6 62.0 70.9 67.7 67.4 57.1 58.4 C C C 62.9 68.9 59.5 C 61.5 

 3541 74.6 S 81.9 70.5 72.4 79.2 79.1 67.8 74.8 C C 72.4 72.4 75.6 

 3542 82.9 83.5 87.0 C 88.7 86.8 C C C 86.7 85.8 77.0 73.6 80.4 79.2 

 3543 69.6 72.1 64.1 78.9 69.4 74.6 69.3 68.3 64.6 C 63.1 C C 71.9 

 3544 70.9 81.4 79.6 78.7 76.8 76.2 C 32.8 71.0 68.8 72.1 C C 71.6 

 3545 71.5 70.8 76.9 79.1 D C C 79.2 C 73.5 C 68.6 64.2 66.1 64.9 C 72.0 

 3546 85.2 88.5 90.6 92.8 84.0 92.9 C C 75.4 C 82.2 81.8 C 86.4 C 77.6 

 3547 75.3 S Z 77.8 71.5 81.4 C 80.9 73.2 72.4 77.0 75.0 C 77.0 66.6 C 

 3548 TOTAL LITTER LOSS 

 3549 79.8 C C 84.9 83.6 82.5 85.5 79.7 C C 77.8 80.2 74.1 74.8 75.3 

 3550 NOT PREG., NO MATING DATE 

 3551 106.0 103.2 117.1 105.2 102.6 106.4 101.7 

MEAN 76.5

S.D. 9.45

N 20

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED M-MISSING 
23-JUN-2009 19:46

Huntingdon Life Sciences 00-4206 PAGE 406 

 

 APPENDIX U 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 64.2 C C C 69.5 67.7 70.5 61.4 65.4 66.9 C 64.0 58.4 C 59.3 58.5 

 4527 72.1 C C 74.2 69.9 C 71.1 72.8 79.4 D 68.6 67.5 69.3 72.9 74.9 

 4528 64.7 62.6 67.5 61.2 75.2 C C M 62.2 63.6 C C 63.8 63.6 M 62.4 M 

 4529 66.2 C C 72.8 70.4 74.2 63.1 62.8 69.1 63.4 57.7 61.4 67.2 

 4530 NOT PREGNANT 

 4531 65.2 64.9 65.8 61.4 C 70.1 67.9 66.4 66.5 63.7 64.6 C C 61.1 

 4532 68.5 D 73.1 72.5 71.2 78.9 59.5 70.4 D 71.6 73.2 D 61.9 D 52.3 D D D 

 4533 83.5 87.8 83.8 82.9 83.2 84.8 87.0 C 80.4 C C C 81.2 78.4 85.8 C 

 4534 71.2 72.4 76.2 77.6 C C 81.7 C 69.7 C C C 73.6 64.2 63.6 63.2 70.1 C 

 4535 70.7 81.4 78.3 70.9 69.4 62.8 59.6 78.5 67.5 68.2 

 4536 71.7 71.4 77.1 75.5 73.4 C 65.9 C 72.9 67.3 71.1 D C 73.8 68.3 C 

 4537 78.1 72.5 87.3 74.3 76.0 76.1 72.4 73.9 89.2 C 73.0 85.8 

 4538 72.0 D 75.9 72.9 76.7 69.8 75.9 C C C C C 72.0 71.3 63.2 73.6 68.3 

 4539 NOT PREGNANT 

 4540 65.3 C 64.3 66.1 65.2 67.5 67.6 64.9 61.1 63.8 67.3 65.6 C 

 4541 NOT PREG., NO MATING DATE 

 4542 71.6 74.9 62.8 73.2 78.6 73.1 70.3 C C 76.6 68.6 70.9 67.0 

 4543 75.2 77.0 78.6 C 77.3 75.9 74.0 76.4 69.8 C 73.6 73.5 76.0 C C C 

 4544 75.5 81.6 C 79.4 74.9 75.7 C C 80.8 76.7 68.5 73.3 C C 73.5 70.7 

 4545 77.9 76.3 78.8 81.2 C 86.4 C C 76.2 76.7 81.6 72.9 72.7 75.8 

 4546 76.2 78.8 C C 79.5 C C 77.6 79.1 81.9 C 72.5 74.2 C 71.3 72.2 74.6 

 4547 69.3 74.7 69.0 72.6 72.7 72.2 C 63.2 C C C 64.9 69.8 67.9 65.6 

 4548 78.0 87.5 71.6 76.5 77.3 84.3 C C 73.7 76.3 81.6 75.4 C 75.3 C C 

 4549 FOUND DEAD 

 4550 77.7 D C 79.1 85.3 84.4 C 80.0 78.8 73.4 83.7 C 72.3 C 73.6 66.3 C 

 4551 67.9 69.7 67.4 67.4 65.7 69.7 73.6 C 62.8 C 66.9 71.3 64.8 

MEAN 71.9

S.D. 5.30

N 22

PUP STATUS CODES: D-DIED C-CULLED M-MISSING 
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 407 

 

 APPENDIX V 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 

ANIMAL # ORGAN OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 ESTROUS STAGE DIESTRUS

 1527 DPP ESTROUS STAGE a 

 1528 ESTROUS STAGE DIESTRUS

 1529 PNMD ESTROUS STAGE DIESTRUS

 1530 ESTROUS STAGE DIESTRUS

 1531 ESTROUS STAGE METESTRUS

 1532 NP ESTROUS STAGE ESTRUS

 1533 ESTROUS STAGE DIESTRUS

 1534 ESTROUS STAGE ESTRUS

 1535 NP ESTROUS STAGE DIESTRUS

 1536 ESTROUS STAGE DIESTRUS

 1537 ESTROUS STAGE DIESTRUS

 1538 ESTROUS STAGE DIESTRUS

 1539 ESTROUS STAGE DIESTRUS

 1540 ESTROUS STAGE DIESTRUS

 1541 NP ESTROUS STAGE ESTRUS

 1542 ESTROUS STAGE DIESTRUS

 1543 ESTROUS STAGE DIESTRUS

 1544 ESTROUS STAGE DIESTRUS

 1545 ESTROUS STAGE DIESTRUS

 1546 ESTROUS STAGE DIESTRUS

 1547 ESTROUS STAGE DIESTRUS

 1548 NNMD ESTROUS STAGE DIESTRUS

 1549 ESTROUS STAGE DIESTRUS

 1550 NNMD ESTROUS STAGE DIESTRUS

 1551 ESTROUS STAGE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

a

No vaginal smear taken for this animal (found dead on Lactation Day 1).
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 408 

 

 APPENDIX V 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 

ANIMAL # ORGAN OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 ESTROUS STAGE PROESTRUS

 2527 NNMD ESTROUS STAGE ESTRUS

 2528 ESTROUS STAGE DIESTRUS

 2529 ESTROUS STAGE DIESTRUS

 2530 ESTROUS STAGE PROESTRUS

 2531 ESTROUS STAGE DIESTRUS

 2532 NP ESTROUS STAGE ESTRUS

 2533 ESTROUS STAGE DIESTRUS

 2534 ESTROUS STAGE DIESTRUS

 2535 ESTROUS STAGE DIESTRUS

 2536 ESTROUS STAGE DIESTRUS

 2537 ESTROUS STAGE DIESTRUS

 2538 NNMD ESTROUS STAGE DIESTRUS

 2539 ESTROUS STAGE DIESTRUS

 2540 ESTROUS STAGE DIESTRUS

 2541 ESTROUS STAGE DIESTRUS

 2542 NNMD ESTROUS STAGE ESTRUS

 2543 ESTROUS STAGE DIESTRUS

 2544 ESTROUS STAGE DIESTRUS

 2545 ESTROUS STAGE ESTRUS

 2546 ESTROUS STAGE ESTRUS

 2547 ESTROUS STAGE DIESTRUS

 2548 ESTROUS STAGE DIESTRUS

 2549 TLL ESTROUS STAGE ESTRUS

 2550 ESTROUS STAGE ESTRUS

 2551 ESTROUS STAGE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 409 

 

 APPENDIX V 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 

ANIMAL # ORGAN OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NP ESTROUS STAGE PROESTRUS

 3527 ESTROUS STAGE DIESTRUS

 3528 ESTROUS STAGE DIESTRUS

 3529 ESTROUS STAGE ESTRUS

 3530 ESTROUS STAGE DIESTRUS

 3531 ESTROUS STAGE DIESTRUS

 3532 ESTROUS STAGE DIESTRUS

 3533 ESTROUS STAGE DIESTRUS

 3534 ESTROUS STAGE DIESTRUS

 3535 ESTROUS STAGE DIESTRUS

 3536 TLL ESTROUS STAGE DIESTRUS

 3537 ESTROUS STAGE DIESTRUS

 3538 NNMD ESTROUS STAGE ESTRUS

 3539 NNMD ESTROUS STAGE ESTRUS

 3540 ESTROUS STAGE DIESTRUS

 3541 ESTROUS STAGE DIESTRUS

 3542 ESTROUS STAGE DIESTRUS

 3543 ESTROUS STAGE METESTRUS

 3544 ESTROUS STAGE DIESTRUS

 3545 ESTROUS STAGE DIESTRUS

 3546 ESTROUS STAGE DIESTRUS

 3547 ESTROUS STAGE DIESTRUS

 3548 TLL ESTROUS STAGE DIESTRUS

 3549 ESTROUS STAGE DIESTRUS

 3550 NNMD ESTROUS STAGE DIESTRUS

 3551 ESTROUS STAGE PROESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS
23-JUN-2009 19:01

Huntingdon Life Sciences 00-4206 PAGE 410 

 

 APPENDIX V 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 

ANIMAL # ORGAN OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 ESTROUS STAGE ESTRUS

 4527 ESTROUS STAGE DIESTRUS

 4528 ESTROUS STAGE DIESTRUS

 4529 ESTROUS STAGE DIESTRUS

 4530 ESTROUS STAGE DIESTRUS

 4531 ESTROUS STAGE DIESTRUS

 4532 ESTROUS STAGE DIESTRUS

 4533 ESTROUS STAGE DIESTRUS

 4534 ESTROUS STAGE DIESTRUS

 4535 ESTROUS STAGE ESTRUS

 4536 ESTROUS STAGE DIESTRUS

 4537 ESTROUS STAGE DIESTRUS

 4538 ESTROUS STAGE PROESTRUS

 4539 NP ESTROUS STAGE DIESTRUS

 4540 ESTROUS STAGE DIESTRUS

 4541 NNMD ESTROUS STAGE ESTRUS

 4542 ESTROUS STAGE DIESTRUS

 4543 ESTROUS STAGE DIESTRUS

 4544 ESTROUS STAGE DIESTRUS

 4545 ESTROUS STAGE DIESTRUS

 4546 ESTROUS STAGE DIESTRUS

 4547 PNMD ESTROUS STAGE PROESTRUS

 4548 ESTROUS STAGE DIESTRUS

 4549 DIED ESTROUS STAGE b 

 4550 ESTROUS STAGE DIESTRUS

 4551 ESTROUS STAGE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

b

Animal died before mating.
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 411 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 NO REMARKABLE OBSERVATIONS 

 1027 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 1028 ABDOMINAL CAVITY DISCOLORED LYMPH NODE(S); MODERATE; RIGHT 

 PERI-RENAL, RED 

TISSUES SAVED 

 1029 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 1030 NO REMARKABLE OBSERVATIONS 

 1031 NO REMARKABLE OBSERVATIONS 

 1032 NO REMARKABLE OBSERVATIONS 

 1033 NO REMARKABLE OBSERVATIONS 

 1034 NO REMARKABLE OBSERVATIONS 

 1035 NO REMARKABLE OBSERVATIONS 

 1036 NO REMARKABLE OBSERVATIONS 

 1037 NO REMARKABLE OBSERVATIONS 

 1038 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 1039 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 AND DIAPHRAGMATIC LOBES - 0.1 CM IN DIAMETER, TAN 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 412 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1040 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 AND DIAPHRAGMATIC LOBE, TAN, 0.1 CM IN DIAMETER 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM ALOPECIA GENERAL 

 EXTERNAL FINDING NOT CONFIRMED 

 1041 NO REMARKABLE OBSERVATIONS 

 1042 GONADS TESTE(S) SMALL; SLIGHT; LEFT 

 GONADS TESTE(S) SOFT; MODERATE; LEFT 

GONADS EPIDIDYMIS(IDES) SMALL; SLIGHT; LEFT 

 1043 THYMUS DISCOLORED FOCI; SLIGHT; LEFT 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 1044 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 1045 NO REMARKABLE OBSERVATIONS 

 1046 NO REMARKABLE OBSERVATIONS 

 1047 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 1048 KIDNEY IRREGULAR SURFACE; SLIGHT; LEFT 

 ROUGH 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 413 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1048 (CONTINUED) 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 1049 NO REMARKABLE OBSERVATIONS 

 1050 NO REMARKABLE OBSERVATIONS 

 1051 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 414 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 2027 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT 

 EXTERNAL FINDINGS CONFIRMED 

 2028 NO REMARKABLE OBSERVATIONS 

 2029 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, RED, LESS THEN 0.1 CM IN DIAMETER, 

 2030 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - 0.1 CM IN DIAMETER, TAN 

 BLADDER CALCULI; MODERATE 

 WHITE, 0.5 CM IN DIAMETER, FIRM 

TISSUE SAVED 

 2031 THYMUS DISCOLORED FOCI; EXTREME; BILATERAL 

 RED, 0.2 CM IN DIAMETER 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1-0.2 CM IN DIAMETER 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 2032 THYMUS DISCOLORED FOCI; MODERATE; RIGHT 

 LESS THAN 0.1 CM IN DIAMETER, RED 

TISSUE SAVED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 2033 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 415 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2034 NO REMARKABLE OBSERVATIONS 

 2035 NO REMARKABLE OBSERVATIONS 

 2036 NO REMARKABLE OBSERVATIONS 

 2037 NO REMARKABLE OBSERVATIONS 

 2038 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 2039 NO REMARKABLE OBSERVATIONS 

 2040 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER 

 2041 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - 0.1 CM IN DIAMETER, TAN 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 2042 THYMUS DISCOLORED FOCI; EXTREME; LEFT 

 RED, 0.1-0.2 CM IN DIAMETER 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 2043 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 416 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2044 NO REMARKABLE OBSERVATIONS 

 2045 NO REMARKABLE OBSERVATIONS 

 2046 NO REMARKABLE OBSERVATIONS 

 2047 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 2048 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 2049 NO REMARKABLE OBSERVATIONS 

 2050 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 2051 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 417 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 NO REMARKABLE OBSERVATIONS 

 3027 KIDNEY DILATED RENAL PELVIS; EXTREME; RIGHT 

 3028 LUNGS DISCOLORED FOCI; SLIGHT 

 RIGHT DIAPHRAGMATIC LOBE, TAN, 0.1 CM IN DIAMETER 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; BILATERAL 

 3029 NO REMARKABLE OBSERVATIONS 

 3030 NO REMARKABLE OBSERVATIONS 

 3031 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER 

 3032 NO REMARKABLE OBSERVATIONS 

 3033 NO REMARKABLE OBSERVATIONS 

 3034 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 3035 NO REMARKABLE OBSERVATIONS 

 3036 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING NOT CONFIRMED 

 3037 NO REMARKABLE OBSERVATIONS 

 3038 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 418 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3039 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - 0.1 CM IN DIAMETER, TAN 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 3040 NO REMARKABLE OBSERVATIONS 

 3041 THYMUS DISCOLORED FOCI; MODERATE; RIGHT 

 RED, LESS THAN 0.1 CM IN DIAMETER, 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, LESS THEN 0.1 CM IN DIAMETER, TAN 

 3042 NO REMARKABLE OBSERVATIONS 

 3043 THYMUS DISCOLORED FOCI; SLIGHT; BILATERAL 

 RED, LESS THAN 0.1 CM IN DIAMETER 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, WHITE, 0.1 CM IN DIAMETER 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 3044 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; EXTREME 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM ALOPECIA GENERAL 

 EXTERNAL FINDING CONFIRMED 

 3045 NO REMARKABLE OBSERVATIONS 

 3046 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 FILLED WITH THIN RED FLUID 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 419 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3047 NO REMARKABLE OBSERVATIONS 

 3048 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 3049 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 3050 THYMUS DISCOLORED FOCI; SLIGHT 

 BOTH LOBES, RED, LESS THEN 0.1 CM IN DIAMETER 

TISSUE SAVED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT 

 EXTERNAL FINDING CONFIRMED 

 3051 THYMUS DISCOLORED FOCI; SLIGHT; BILATERAL 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING NOT CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 420 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 NO REMARKABLE OBSERVATIONS 

 4027 NO REMARKABLE OBSERVATIONS 

 4028 NO REMARKABLE OBSERVATIONS 

 4029 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 4030 NO REMARKABLE OBSERVATIONS 

 4031 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN O.1 CM IN DIAMETER, TAN 

 LEFT LOBE - DISCOLORED FOCUS - 0.2 CM IN DIAMETER, RED 

 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 4032 NO REMARKABLE OBSERVATIONS 

 4033 NO REMARKABLE OBSERVATIONS 

 4034 NO REMARKABLE OBSERVATIONS 

 4035 THYMUS DISCOLORED FOCI; MODERATE; BILATERAL 

 RED, 0.1 CM IN DIAMETER 

 TISSUE SAVED 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 4052 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 421 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4037 KIDNEY DILATED RENAL PELVIS; SLIGHT; LEFT 

 4038 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; MODERATE 

 EXTERNAL FINDING CONFIRMED 

 4039 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THEN O.1 CM IN DIAMETER 

 4040 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 4041 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 4042 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 4043 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT 

 EXTERNAL FINDING CONFIRMED 

 4044 THYMUS DISCOLORED FOCI; SLIGHT; BILATERAL 

 RED, LESS THAN 0.1 CM IN DIAMETER 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, LESS THAN 0.1 CM IN DIAMETER, TAN 

 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 FILLED WITH THIN RED FLUID 

 4045 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER 

 4046 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:25

Huntingdon Life Sciences 00-4206 PAGE 422 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4046 (CONTINUED) 

 GONADS UNDESCENDED TESTE(S); LEFT 

 4047 THYMUS DISCOLORED FOCI; EXTREME; BILATERAL 

 RED, 0.1-0.2 CM IN DIAMETER 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT 

 EXTERNAL FINDING CONFIRMED 

 4048 NO REMARKABLE OBSERVATIONS 

 4049 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - O.1 CM IN DIAMETER, TAN 

 4050 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 4051 THYMUS DISCOLORED FOCI; EXTREME; BILATERAL 

 0.2 CM IN DIAMETER, RED 

 TISSUE SAVED 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 423 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 NO REMARKABLE OBSERVATIONS 

 1527 DPP THYMUS DISCOLORED FOCI; MODERATE; BILATERAL 

 RED - 0.1 CM IN DIAMETER 

 TISSUE SAVED 

 ADRENALS DISCOLORED ADRENALS; EXTREME; BILATERAL 

 RED 

 UTERUS FETUS(ES) IN UTERUS 

 ONE FETUS IN LEFT HORN 

EXTERNAL EXAM - WITHIN NORMAL LIMITS 

 1528 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 1529 PNMD NO REMARKABLE OBSERVATIONS 

 1530 NO REMARKABLE OBSERVATIONS 

 1531 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 1532 NP SALEWSKI'S TEST NO FOCI PRESENT 

 1533 OVARY CYST(S); LEFT 

 1.5 CM IN DIAMETER, CLEAR 

 1534 NO REMARKABLE OBSERVATIONS 

 1535 NP LIVER ADHESION 

 MEDIAN LOBE TO DIAPHRAGM - 1.5 CM IN DIAMETER 

 TISSUE SAVED 

 GONADS FLUID-FILLED VAGINA 

 1.5 X 1.0 X 1.0 CM, THICK WHITE FLUID 

TISSUE SAVED 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 424 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1535 (CONTINUED) 

 SALEWSKI'S TEST NO FOCI PRESENT 

 1536 LIVER ADHESION 

 BETWEEN RIGHT LOBES - EXTREME 

 LIVER MASS; RIGHT 

 1.5 X 0.7 CM, RED, FIRM 

 1537 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 1538 NO REMARKABLE OBSERVATIONS 

 1539 NO REMARKABLE OBSERVATIONS 

 1540 NO REMARKABLE OBSERVATIONS 

 1541 NP SALEWSKI'S TEST NO FOCI PRESENT 

 1542 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 O.2 CM IN DIAMETER, RED 

 KIDNEY MASS; LEFT 

 4.O X 3.0 CM, TAN, FIRM 

 1543 NO REMARKABLE OBSERVATIONS 

 1544 NO REMARKABLE OBSERVATIONS 

 1545 NO REMARKABLE OBSERVATIONS 

 1546 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 425 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1547 NO REMARKABLE OBSERVATIONS 

 1548 NNMD SALEWSKI'S TEST NO FOCI PRESENT 

 1549 NO REMARKABLE OBSERVATIONS 

 1550 NNMD SALEWSKI'S TEST NO FOCI PRESENT 

 1551 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 426 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 ABDOMINAL CAVITY MASS; MODERATE; RIGHT 

 RED, FIRM, 0.8 CM X 1.5 CM 

ADHESION TO RENAL LYMPH NODE, RIGHT KIDNEY, RIGHT ADRENAL, AND

ACCESSORY LOBE OF LIVER 

TISSUE SAVED 

 ADRENALS DISCOLORED ADRENALS; MODERATE; RIGHT 

 RED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 2527 NNMD SALEWSKI'S TEST NO FOCI PRESENT 

 2528 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 2529 NO REMARKABLE OBSERVATIONS 

 2530 NO REMARKABLE OBSERVATIONS 

 2531 NO REMARKABLE OBSERVATIONS 

 2532 NP EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT 

 EXTERNAL FINDING CONFIRMED 

 SALEWSKI'S TEST NO FOCI PRESENT 

 2533 NO REMARKABLE OBSERVATIONS 

 2534 NO REMARKABLE OBSERVATIONS 

 2535 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, WHITE, LESS THAN 0.1 CM IN DIAMETER, 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 427 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2536 NO REMARKABLE OBSERVATIONS 

 2537 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 2538 NNMD SALEWSKI'S TEST NO FOCI PRESENT 

 2539 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 2540 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 2541 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 2542 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 2543 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - 0.2 CM IN DIAMETER, RED 

 2544 NO REMARKABLE OBSERVATIONS 

 2545 NO REMARKABLE OBSERVATIONS 

 2546 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 428 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2547 NO REMARKABLE OBSERVATIONS 

 2548 NO REMARKABLE OBSERVATIONS 

 2549 TLL NO REMARKABLE OBSERVATIONS 

 2550 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 TAN, LESS THAN 0.1 CM IN DIAMETER 

 2551 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------

TLL=TOTAL LITTER LOSS 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 429 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NP SALEWSKI'S TEST NO FOCI PRESENT 

 3527 NO REMARKABLE OBSERVATIONS 

 3528 NO REMARKABLE OBSERVATIONS 

 3529 EXTERNAL EXAM EAR(S) SWOLLEN/RED 

 EXTERNAL FINDING CONFIRMED 

TISSUE SAVED 

 3530 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 3531 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 3532 NO REMARKABLE OBSERVATIONS 

 3533 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 3534 NO REMARKABLE OBSERVATIONS 

 3535 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 RED, SLIGHT, 0.1 CM IN DIAMETER 

 3536 TLL EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT TLL=TOTAL LITTER LOSS 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 430 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3537 NO REMARKABLE OBSERVATIONS 

 3538 NNMD SALEWSKI'S TEST NO FOCI PRESENT 

 3539 NNMD LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 SALEWSKI'S TEST NO FOCI PRESENT 

 3540 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 3541 NO REMARKABLE OBSERVATIONS 

 3542 NO REMARKABLE OBSERVATIONS 

 3543 NO REMARKABLE OBSERVATIONS 

 3544 NO REMARKABLE OBSERVATIONS 

 3545 NO REMARKABLE OBSERVATIONS 

 3546 NO REMARKABLE OBSERVATIONS 

 3547 NO REMARKABLE OBSERVATIONS 

 3548 TLL NO REMARKABLE OBSERVATIONS 

 3549 NO REMARKABLE OBSERVATIONS 

 3550 NNMD SALEWSKI'S TEST NO FOCI PRESENT 

 3551 ADRENALS DISCOLORED FOCI; SLIGHT; LEFT 

 0.1 CM IN DIAMETER, RED 

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 431 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 4527 NO REMARKABLE OBSERVATIONS 

 4528 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 4529 NO REMARKABLE OBSERVATIONS 

 4530 NP SALEWSKI'S TEST NO FOCI PRESENT 

 4531 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 4532 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 4533 NO REMARKABLE OBSERVATIONS 

 4534 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 4535 NO REMARKABLE OBSERVATIONS 

 4536 NO REMARKABLE OBSERVATIONS 

 4537 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 4538 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 432 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4539 NP SALEWSKI'S TEST NO FOCI PRESENT 

 4540 NO REMARKABLE OBSERVATIONS 

 4541 NNMD LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 SALEWSKI'S TEST NO FOCI PRESENT 

 4542 NO REMARKABLE OBSERVATIONS 

 4543 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 4544 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, WHITE, LESS THAN 0.1 CM IN DIAMETER 

 4545 NO REMARKABLE OBSERVATIONS 

 4546 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

 4547 PNMD LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN AND RED, 0.1 CM IN DIAMETER 

 4548 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 LEFT-SLIGHT 

 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, 0.1 CM IN DIAMETER, TAN 

 4549 DIED THYMUS DISCOLORED FOCI; MODERATE; BILATERAL 

 RED, 0.2 CM IN DIAMETER 

TISSUE SAVED 

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE 
23-JUN-2009 20:26

Huntingdon Life Sciences 00-4206 PAGE 433 

 

 APPENDIX W 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4549 (CONTINUED) 

 LUNGS DISCOLORED FOCI; EXTREME 

 ALL LOBES, RED, 0.2 CM IN DIAMETER 

 4550 NO REMARKABLE OBSERVATIONS 

 4551 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER 

-----------------------------------------------------------------------------------------------------------------------------------
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 434 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 1F S STOMACH NO MILK IN STOMACH 

 LUNGS LUNG FLOATATION TEST - STILLBORN 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 1527 DPP 1F Da 0 LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH MILK IN STOMACH 

 2F Da 0 LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH MILK IN STOMACH 

 3M U 1 NO REMARKABLE OBSERVATIONS 

 4M U 1 NO REMARKABLE OBSERVATIONS 

 5M U 1 NO REMARKABLE OBSERVATIONS 

 6F U 1 NO REMARKABLE OBSERVATIONS 

 7F U 1 NO REMARKABLE OBSERVATIONS 

 8F U 1 NO REMARKABLE OBSERVATIONS 

 9F U 1 NO REMARKABLE OBSERVATIONS 

 10F U 1 NO REMARKABLE OBSERVATIONS 

 11F U 1 NO REMARKABLE OBSERVATIONS 

 12F U 1 NO REMARKABLE OBSERVATIONS 

 13F U 1 NO REMARKABLE OBSERVATIONS 

 14F U 1 NO REMARKABLE OBSERVATIONS 

 1528 1M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE U-UNSCHEDULED SACRIFICE 

DPP=DIED POST PARTUM, LITTER EXCLUDED a

Lung floatation test result superseded by observation of milk in stomach.
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 435 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1528 (CONTINUED) 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 1529 PNMD 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 1530 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 6M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); SLIGHT; RIGHT 

 TISSUE SAVED 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 1531 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 

PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 436 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1531 (CONTINUED) 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 1533 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 1534 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 437 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1536 1M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 1537 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 1538 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUE SAVED 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 438 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1539 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 1540 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 1542 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 439 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1543 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 1544 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F D 9 STOMACH MILK IN STOMACH 

 1545 2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 440 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1545 (CONTINUED) 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 1546 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 1547 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11M C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 1549 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:28

Huntingdon Life Sciences 00-4206 PAGE 441 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1549 (CONTINUED) 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 1551 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 442 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 1M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2F D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH MILK IN STOMACH 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2528 1M S GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 2M K 28 GONADS SMALL TESTE(S); MODERATE; BILATERAL 

 TISSUE SAVED 

 GONADS SMALL EPIDIDYMIS(IDES); MODERATE; BILATERAL 

 TISSUE SAVED 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 2529 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29 

Huntingdon Life Sciences 00-4206 PAGE 443 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2529 (CONTINUED) 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 2530 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 2531 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 2533 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 444 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2533 (CONTINUED) 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 2534 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3F K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 2535 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 2536 1M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F D 3 STOMACH MILK IN STOMACH 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 445 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2536 (CONTINUED) 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 2537 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 2539 1F D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2540 1F Z GROSS EXAM CANNIBALIZED 

 GROSS EXAM SEX UNDETERMINED 

 2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 446 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2541 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2543 1F D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 2544 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3F K 28 NO REMARKABLE OBSERVATIONS 

 4F K 28 NO REMARKABLE OBSERVATIONS 

 2545 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 447 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2545 (CONTINUED) 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 2546 1F S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2547 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 2548 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:29

Huntingdon Life Sciences 00-4206 PAGE 448 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2550 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 2551 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 449 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 1M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); SLIGHT; RIGHT 

 TISSUE SAVED 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT 

 TISSUE SAVED 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 17F K 28 NO REMARKABLE OBSERVATIONS 

 18F K 28 NO REMARKABLE OBSERVATIONS 

 3528 1M D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5F D 4 STOMACH NO MILK IN STOMACH 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 3529 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 450 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3529 (CONTINUED) 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3530 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 3531 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 3532 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 451 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3532 (CONTINUED) 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3533 1F S GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); SLIGHT; RIGHT 

 TISSUE SAVED 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 3534 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F D 4 STOMACH MILK IN STOMACH 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 3535 2M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT 

 TISSUE SAVED 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 452 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3535 (CONTINUED) 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 3536 TLL 1U Z GROSS EXAM CANNIBALIZED 

 GROSS EXAM SEX UNDETERMINED 

 2U Z GROSS EXAM CANNIBALIZED 

 GROSS EXAM SEX UNDETERMINED 

 3M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 4M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 5M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 6F S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 7F D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 8F S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 9F S GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 10F D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 3537 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED K-SCHEDULED SACRIFICE 

TLL=TOTAL LITTER LOSS 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 453 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3537 (CONTINUED) 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 10M K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 3540 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 3541 1M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 454 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3542 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 10M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3543 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4F K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 3544 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 455 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3545 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M D 2 STOMACH MILK IN STOMACH 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 3546 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 3547 1M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M Z 1 GROSS EXAM CANNIBALIZED 

 GROSS EXAM SEX UNDETERMINED 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT 

 TISSUE SAVED 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED Z-CANNIBALIZED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 456 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3547 (CONTINUED) 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 3548 TLL 1M S LUNGS LUNG FLOATATION TEST - STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M Z 1 GROSS EXAM CANNIBALIZED 

 PARTIALLY 

 GROSS EXAM SEX UNDETERMINED 

STOMACH MILK IN STOMACH UNDETERMINED 

 3M Z 1 GROSS EXAM CANNIBALIZED 

 PARTIALLY 

 GROSS EXAM SEX UNDETERMINED 

STOMACH MILK IN STOMACH UNDETERMINED 

 9F Z 1 GROSS EXAM CANNIBALIZED 

 PARTIALLY 

 GROSS EXAM SEX UNDETERMINED 

 STOMACH MILK IN STOMACH UNDETERMINED 

 10F Z 1 GROSS EXAM CANNIBALIZED 

 PARTIALLY 

 GROSS EXAM SEX UNDETERMINED 

STOMACH MILK IN STOMACH UNDETERMINED 

 12F Z 1 GROSS EXAM CANNIBALIZED 

 PARTIALLY 

 GROSS EXAM SEX UNDETERMINED 

 STOMACH MILK IN STOMACH UNDETERMINED 

 3549 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED Z-CANNIBALIZED K-SCHEDULED SACRIFICE 

TLL=TOTAL LITTER LOSS 
23-JUN-2009 20:30

Huntingdon Life Sciences 00-4206 PAGE 457 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3549 (CONTINUED) 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 3551 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 458 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 4527 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F D 3 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 4528 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11M C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 459 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4529 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 4531 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 4532 1M D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8F D 1 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 GROSS EXAM CANNIBALIZED 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 460 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4532 (CONTINUED) 

 STOMACH NO MILK IN STOMACH 

 16F D 1 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 17F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 4533 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 4534 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11M C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 17F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 461 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4535 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 4536 1M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT 

 TISSUE SAVED 

 2M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUE SAVED 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F D 4 STOMACH NO MILK IN STOMACH 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 4537 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 4538 1F D 0 LUNGS LUNG FLOATATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 462 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4538 (CONTINUED) 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11M C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 4540 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 4542 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 4543 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 463 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4543 (CONTINUED) 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 4544 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT 

 TISSUE SAVED 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 4545 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 10M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 4546 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 464 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4546 (CONTINUED) 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 4547 PNMD 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 4548 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 4550 1M D 4 STOMACH NO MILK IN STOMACH 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 

PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:31

Huntingdon Life Sciences 00-4206 PAGE 465 

 

 APPENDIX X 

 GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4550 (CONTINUED) 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 4551 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
Huntingdon Life Sciences 00-4206 Page 466 

211-TBA-1G Final Report 

Individual Parental 

 Organ and Final Body Weights and Ratios 

Preface Appendix Y 

Key to Abbreviations: 

g = Grams 

SEM. VES. & CO. GL. = Seminal Vesicles and Coagulating Gland 

Corresponding exposure levels for each group were as follows: 

Group 1 - 0 mg/m3

Group 2 - 2000 mg/m3

Group 3 - 10000 mg/m3 

Group 4 - 20000 mg/m3
23-JUN-2009 20:39 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ TESTIS - RIGHT TESTIS - LEFT TESTES EPID Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 1026 542 1.6775 0.0031 0.8559 1.7092 0.0032 0.8720 3.3663 0.0062 1.7175 0.6914  1027 525 1.7957 0.0034 0.8581 1.8504 0.0035 0.8842 3.6221 0.0069 1.7308 0.7072  1028 542 1.6472 0.0030 0.8071 1.6779 0.0031 0.8222 3.3027 0.0061 1.6183 0.6588  1029 564 1.8337 0.0033 0.7928 1.8113 0.0032 0.7831 3.6387 0.0065 1.5732 0.6703  1030 614 1.7799 0.0029 0.7614 1.7344 0.0028 0.7419 3.4934 0.0057 1.4943 0.8438  1031 528 1.8174 0.0034 0.8606 1.8857 0.0036 0.8929 3.6881 0.0070 1.7464 0.7412  1032 518 1.5362 0.0030 0.7109 1.5810 0.0031 0.7317 3.1007 0.0060 1.4350 0.6475  1033 751 2.0144 0.0027 0.8732 2.1401 0.0028 0.9277 4.1334 0.0055 1.7918 0.8605  1034 610 1.8013 0.0030 0.8520 1.7688 0.0029 0.8367 3.5437 0.0058 1.6762 0.7314  1035 568 1.8811 0.0033 0.9111 1.8295 0.0032 0.8861 3.6990 0.0065 1.7915 0.7648  1036 547 1.9700 0.0036 0.9035 1.9397 0.0035 0.8896 3.8845 0.0071 1.7816 0.6719  1037 576 1.9895 0.0035 0.9305 2.0247 0.0035 0.9469 3.9868 0.0069 1.8646 0.7785  1038 598 1.8070 0.0030 0.7960 1.8445 0.0031 0.8125 3.6340 0.0061 1.6008 0.7194  1039 548 1.5714 0.0029 0.7671 1.5326 0.0028 0.7481 3.0998 0.0057 1.5131 0.6665  1040 665 1.8513 0.0028 0.8780 1.7928 0.0027 0.8502 3.6206 0.0054 1.7171 0.7602  1041 564 1.5634 0.0028 0.7665 1.5697 0.0028 0.7696 3.1172 0.0055 1.5283 0.7182  1042 595 1.7805 0.0030 0.8321 0.9571 0.0016 0.4473 2.7309 0.0046 1.2763 0.7795  1043 582 1.6597 0.0029 0.7601 1.6597 0.0029 0.7601 3.3021 0.0057 1.5124 0.7456  1044 550 1.8219 0.0033 0.8653 1.8634 0.0034 0.8850 3.6689 0.0067 1.7424 0.6967  1045 451 1.6719 0.0037 0.8174 1.7057 0.0038 0.8339 3.3692 0.0075 1.6472 0.7198  1046 545 1.9943 0.0037 0.9200 1.9373 0.0036 0.8938 3.9129 0.0072 1.8052 0.8787  1047 603 1.8357 0.0030 0.8714 1.8692 0.0031 0.8873 3.6885 0.0061 1.7508 0.7541  1048 487 1.6826 0.0035 0.8289 1.6291 0.0033 0.8025 3.3025 0.0068 1.6268 0.6643  1049 556 1.7775 0.0032 0.8282 1.7990 0.0032 0.8382 3.5629 0.0064 1.6601 0.8219  1050 560 1.9336 0.0035 0.8822 1.8884 0.0034 0.8616 3.8223 0.0068 1.7439 0.7487  1051 613 1.9099 0.0031 0.8390 1.8219 0.0030 0.8004 3.7071 0.0060 1.6286 0.7492 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:40 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ TESTIS - RIGHT TESTIS - LEFT TESTES EPID Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 2026 607 1.6941 0.0028 0.7441 1.6565 0.0027 0.7276 3.3340 0.0055 1.4644 0.7434  2027 585 1.6057 0.0027 0.7936 1.6643 0.0028 0.8226 3.2515 0.0056 1.6070 0.7584  2028 589 1.7704 0.0030 0.8151 1.7615 0.0030 0.8110 3.5215 0.0060 1.6213 0.7744  2029 645 1.7032 0.0026 0.7238 1.7232 0.0027 0.7323 3.4130 0.0053 1.4504 0.8355  2030 613 1.8846 0.0031 0.8379 1.8015 0.0029 0.8009 3.6716 0.0060 1.6323 0.7782  2031 552 1.7984 0.0033 0.8024 1.7839 0.0032 0.7960 3.5641 0.0065 1.5903 0.8168  2032 598 1.9493 0.0033 0.8477 1.8694 0.0031 0.8129 3.8133 0.0064 1.6582 0.7606  2033 671 1.8059 0.0027 0.9097 1.8474 0.0028 0.9306 3.6321 0.0054 1.8296 0.6794  2034 573 1.6133 0.0028 0.7636 1.5910 0.0028 0.7530 3.1927 0.0056 1.5111 0.6915  2035 551 1.6294 0.0030 0.7891 1.6923 0.0031 0.8196 3.3063 0.0060 1.6013 0.7380  2036 605 1.7887 0.0030 0.8190 1.8153 0.0030 0.8312 3.5825 0.0059 1.6404 0.7516  2037 558 1.7860 0.0032 0.8413 1.7683 0.0032 0.8330 3.5350 0.0063 1.6653 0.7731  2038 538 1.5582 0.0029 0.7542 1.5816 0.0029 0.7656 3.1261 0.0058 1.5132 0.5949  2039 604 1.9730 0.0033 0.9120 2.0268 0.0034 0.9369 3.9862 0.0066 1.8426 0.7854  2040 531 1.7556 0.0033 0.8167 1.6959 0.0032 0.7889 3.4346 0.0065 1.5977 0.7572  2041 575 2.0638 0.0036 0.9320 2.0127 0.0035 0.9090 4.0674 0.0071 1.8369 0.7748  2042 653 1.9203 0.0029 0.8402 1.9439 0.0030 0.8505 3.8527 0.0059 1.6857 0.8588  2043 553 2.1654 0.0039 0.9826 2.1116 0.0038 0.9582 4.2677 0.0077 1.9366 0.7642  2044 493 1.5646 0.0032 0.7034 1.6059 0.0033 0.7219 3.1588 0.0064 1.4201 0.6267  2045 542 1.7487 0.0032 0.8008 1.7634 0.0033 0.8075 3.5011 0.0065 1.6032 0.8040  2046 596 2.0407 0.0034 0.8824 2.0318 0.0034 0.8785 4.0674 0.0068 1.7587 0.8397  2047 569 1.7547 0.0031 0.7806 1.7229 0.0030 0.7664 3.4621 0.0061 1.5401 0.7599  2048 522 1.6505 0.0032 0.7717 1.6470 0.0032 0.7701 3.2903 0.0063 1.5384 0.7256  2049 539 1.7000 0.0032 0.7884 1.7402 0.0032 0.8070 3.4343 0.0064 1.5926 0.7748  2050 553 1.8422 0.0033 0.9142 1.7230 0.0031 0.8551 3.5599 0.0064 1.7667 0.7050  2051 556 1.7750 0.0032 0.8350 1.7391 0.0031 0.8181 3.5223 0.0063 1.6570 0.8083 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ TESTIS - RIGHT TESTIS - LEFT TESTES EPID Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 3026 470 1.8077 0.0038 0.8812 1.8807 0.0040 0.9167 3.6776 0.0078 1.7926 0.7150  3027 560 1.9940 0.0036 0.9256 1.9662 0.0035 0.9127 3.9495 0.0070 1.8334 0.7203  3028 629 1.6855 0.0027 0.8002 1.7225 0.0027 0.8178 3.4042 0.0054 1.6162 0.7593  3029 578 1.7467 0.0030 0.8147 1.8088 0.0031 0.8437 3.5498 0.0061 1.6558 0.7152  3030 612 1.7683 0.0029 0.8157 1.7762 0.0029 0.8194 3.5260 0.0058 1.6266 0.7672  3031 509 1.7894 0.0035 0.8028 1.7429 0.0034 0.7819 3.5276 0.0069 1.5826 0.7850  3032 492 1.6839 0.0034 0.8095 1.7322 0.0035 0.8327 3.3993 0.0069 1.6340 0.6425  3033 503 1.8313 0.0036 0.8348 1.8700 0.0037 0.8524 3.6859 0.0073 1.6802 0.7452  3034 583 1.6200 0.0028 0.8241 1.6104 0.0028 0.8192 3.2177 0.0055 1.6368 0.6900  3035 480 1.8133 0.0038 0.8991 1.7706 0.0037 0.8780 3.5694 0.0074 1.7699 0.7561  3036 563 1.8143 0.0032 0.8376 1.8395 0.0033 0.8492 3.6414 0.0065 1.6811 0.7604  3037 464 2.0015 0.0043 0.8948 1.9580 0.0042 0.8754 3.9469 0.0085 1.7646 0.7031  3038 576 1.7407 0.0030 0.8374 1.6858 0.0029 0.8110 3.4080 0.0059 1.6396 0.7400  3039 515 1.6915 0.0033 0.7876 1.6885 0.0033 0.7862 3.3639 0.0065 1.5662 0.7287  3040 584 1.9116 0.0033 0.8539 1.9279 0.0033 0.8611 3.8225 0.0066 1.7074 0.8188  3041 633 1.9975 0.0032 0.8795 1.9809 0.0031 0.8722 3.9663 0.0063 1.7463 0.9415  3042 523 1.8043 0.0035 0.8897 1.8187 0.0035 0.8968 3.6080 0.0069 1.7792 0.6817  3043 495 1.8241 0.0037 0.8701 1.7641 0.0036 0.8415 3.5758 0.0072 1.7057 0.9058  3044 595 1.6881 0.0028 0.7606 1.6719 0.0028 0.7533 3.3453 0.0056 1.5072 0.7515  3045 546 1.8612 0.0034 0.8313 1.8379 0.0034 0.8209 3.6862 0.0068 1.6464 0.7477  3046 637 1.9213 0.0030 0.8503 1.8485 0.0029 0.8181 3.7580 0.0059 1.6632 0.7582  3047 553 1.6293 0.0029 0.7630 1.6722 0.0030 0.7831 3.2902 0.0060 1.5408 0.6906  3048 603 1.6465 0.0027 0.7652 1.6631 0.0028 0.7730 3.3032 0.0055 1.5352 0.6651  3049 558 1.6468 0.0030 0.7863 1.6451 0.0029 0.7855 3.3144 0.0059 1.5826 0.6898  3050 589 1.8873 0.0032 0.8342 1.8845 0.0032 0.8330 3.7407 0.0063 1.6535 0.9014  3051 608 1.7028 0.0028 0.7690 1.6659 0.0027 0.7524 3.3616 0.0055 1.5182 0.7328 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ TESTIS - RIGHT TESTIS - LEFT TESTES EPID Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 4026 554 1.7263 0.0031 0.7462 1.7537 0.0032 0.7580 3.4667 0.0063 1.4984 0.7823  4027 503 1.5599 0.0031 0.7258 1.5541 0.0031 0.7231 3.1031 0.0062 1.4438 0.6594  4028 565 1.7875 0.0032 0.8668 1.7637 0.0031 0.8553 3.5403 0.0063 1.7168 0.6965  4029 564 2.0014 0.0035 0.9830 1.9344 0.0034 0.9501 3.9209 0.0069 1.9257 0.7567  4030 557 1.6792 0.0030 0.7959 1.7768 0.0032 0.8422 3.4468 0.0062 1.6337 0.6889  4031 513 1.9754 0.0039 0.9414 1.8831 0.0037 0.8974 3.8533 0.0075 1.8363 0.6962  4032 558 1.8769 0.0034 0.8547 1.8801 0.0034 0.8561 3.7478 0.0067 1.7066 0.6775  4033 561 2.1379 0.0038 1.0467 2.1572 0.0038 1.0561 4.2852 0.0076 2.0979 0.7110  4034 492 1.8611 0.0038 0.9292 1.7740 0.0036 0.8857 3.6213 0.0074 1.8079 0.6824  4035 586 2.1499 0.0037 0.9477 2.1259 0.0036 0.9371 4.2575 0.0073 1.8768 0.7740  4052 522 1.7044 0.0033 0.8013 1.7873 0.0034 0.8403 3.4703 0.0066 1.6315 0.6915  4037 498 1.6462 0.0033 0.8055 1.7005 0.0034 0.8320 3.3314 0.0067 1.6300 0.6047  4038 551 1.7085 0.0031 0.7713 1.6888 0.0031 0.7624 3.3838 0.0061 1.5277 0.7560  4039 534 1.6160 0.0030 0.7498 1.5942 0.0030 0.7397 3.2021 0.0060 1.4857 0.7170  4040 503 2.0579 0.0041 0.9950 2.1043 0.0042 1.0175 4.1492 0.0082 2.0062 0.8116  4041 562 1.6441 0.0029 0.8022 1.6477 0.0029 0.8040 3.2787 0.0058 1.5998 0.6879  4042 492 1.7865 0.0036 0.8797 1.7886 0.0036 0.8807 3.5693 0.0073 1.7576 0.6431  4043 532 1.6007 0.0030 0.7668 1.5841 0.0030 0.7589 3.1792 0.0060 1.5230 0.6885  4044 586 1.8316 0.0031 0.8917 1.8256 0.0031 0.8888 3.6479 0.0062 1.7759 0.7026  4045 590 1.7733 0.0030 0.7805 1.8175 0.0031 0.8000 3.5824 0.0061 1.5768 0.7718  4046 511 1.6875 0.0033 0.7997 1.7226 0.0034 0.8164 3.3976 0.0067 1.6102 0.7120  4047 525 1.6193 0.0031 0.7698 1.5763 0.0030 0.7493 3.1847 0.0061 1.5139 0.7768  4048 515 1.6987 0.0033 0.7793 1.7239 0.0033 0.7909 3.4074 0.0066 1.5632 0.7269  4049 459 1.7213 0.0038 0.8235 1.6985 0.0037 0.8126 3.4138 0.0074 1.6333 0.6218  4050 515 1.9544 0.0038 0.9645 1.9880 0.0039 0.9811 3.9455 0.0077 1.9470 0.7201  4051 587 1.7597 0.0030 0.8378 1.8042 0.0031 0.8589 3.5684 0.0061 1.6988 0.6651 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:39 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM. Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 1026 542 0.6660 0.0012 0.3398 1.3521 0.0025 0.6898 0.9057 0.0017 0.4621 2.6832  1027 525 0.7206 0.0014 0.3443 1.4207 0.0027 0.6789 0.8817 0.0017 0.4213 2.3377  1028 542 0.7365 0.0014 0.3609 1.3754 0.0025 0.6740 0.8784 0.0016 0.4304 2.2008  1029 564 0.6382 0.0011 0.2759 1.2987 0.0023 0.5615 1.0690 0.0019 0.4622 2.5715  1030 614 0.8631 0.0014 0.3692 1.6928 0.0028 0.7241 1.4388 0.0023 0.6155 1.7340  1031 528 0.8036 0.0015 0.3805 1.5308 0.0029 0.7249 0.8645 0.0016 0.4094 2.1202  1032 518 0.6476 0.0013 0.2997 1.2796 0.0025 0.5922 1.0039 0.0019 0.4646 2.0313  1033 751 0.8961 0.0012 0.3885 1.7390 0.0023 0.7539 1.1209 0.0015 0.4859 2.3333  1034 610 0.7235 0.0012 0.3422 1.4371 0.0024 0.6798 1.0890 0.0018 0.5151 1.9893  1035 568 0.7159 0.0013 0.3467 1.4696 0.0026 0.7118 1.1267 0.0020 0.5457 2.6228  1036 547 0.6905 0.0013 0.3167 1.3528 0.0025 0.6205 1.1173 0.0020 0.5125 1.7191  1037 576 0.7965 0.0014 0.3725 1.5591 0.0027 0.7292 1.1906 0.0021 0.5568 2.0920  1038 598 0.7499 0.0013 0.3303 1.4609 0.0024 0.6435 1.2779 0.0021 0.5629 2.8878  1039 548 0.6088 0.0011 0.2972 1.2710 0.0023 0.6204 1.1972 0.0022 0.5844 2.1402  1040 665 0.7573 0.0011 0.3591 1.5067 0.0023 0.7146 1.2602 0.0019 0.5976 2.5024  1041 564 0.6726 0.0012 0.3298 1.3880 0.0025 0.6805 0.9892 0.0018 0.4850 2.1511  1042 595 0.5621 0.0009 0.2627 1.3337 0.0022 0.6233 0.8234 0.0014 0.3848 1.9761  1043 582 0.7194 0.0012 0.3295 1.4581 0.0025 0.6678 1.2367 0.0021 0.5664 2.6118  1044 550 0.7206 0.0013 0.3422 1.4072 0.0026 0.6683 0.9603 0.0017 0.4561 1.9238  1045 451 0.7273 0.0016 0.3556 1.4380 0.0032 0.7030 0.9303 0.0021 0.4548 2.5091  1046 545 0.8515 0.0016 0.3928 1.7163 0.0032 0.7918 1.1426 0.0021 0.5271 2.3376  1047 603 0.7823 0.0013 0.3713 1.5349 0.0025 0.7286 1.0079 0.0017 0.4784 2.6165  1048 487 0.6223 0.0013 0.3066 1.2770 0.0026 0.6291 0.7636 0.0016 0.3762 1.9307  1049 556 0.7397 0.0013 0.3447 1.5521 0.0028 0.7232 1.1567 0.0021 0.5390 2.0049  1050 560 0.6960 0.0012 0.3175 1.4589 0.0026 0.6656 0.9894 0.0018 0.4514 1.9378  1051 613 0.7015 0.0011 0.3082 1.4420 0.0024 0.6335 0.7654 0.0012 0.3362 2.3169 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:40 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM. Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 2026 607 0.7131 0.0012 0.3132 1.4480 0.0024 0.6360 1.1328 0.0019 0.4976 1.8866  2027 585 0.7245 0.0012 0.3581 1.4726 0.0025 0.7278 1.1009 0.0019 0.5441 1.6055  2028 589 0.8070 0.0014 0.3715 1.5725 0.0027 0.7240 1.0919 0.0019 0.5027 2.3523  2029 645 0.8439 0.0013 0.3586 1.6665 0.0026 0.7082 1.5187 0.0024 0.6454 2.2828  2030 613 0.7113 0.0012 0.3162 1.4737 0.0024 0.6552 1.0588 0.0017 0.4707 2.0901  2031 552 0.7482 0.0014 0.3338 1.5430 0.0028 0.6885 1.0254 0.0019 0.4575 2.3120  2032 598 0.7828 0.0013 0.3404 1.5411 0.0026 0.6702 1.4670 0.0025 0.6379 2.6780  2033 671 0.7161 0.0011 0.3607 1.3786 0.0021 0.6944 1.1150 0.0017 0.5617 2.1388  2034 573 0.7155 0.0012 0.3387 1.3940 0.0024 0.6598 1.1615 0.0020 0.5497 1.7326  2035 551 0.7674 0.0014 0.3717 1.4911 0.0027 0.7222 1.3706 0.0025 0.6638 2.2360  2036 605 0.7253 0.0012 0.3321 1.4619 0.0024 0.6694 1.3917 0.0023 0.6373 1.9760  2037 558 0.7478 0.0013 0.3523 1.5110 0.0027 0.7118 0.8153 0.0015 0.3841 2.2832  2038 538 0.5976 0.0011 0.2893 1.1797 0.0022 0.5710 0.8928 0.0017 0.4322 1.9856  2039 604 0.8388 0.0014 0.3877 1.6211 0.0027 0.7494 0.8486 0.0014 0.3923 1.8095  2040 531 0.7005 0.0013 0.3259 1.4516 0.0027 0.6753 1.0540 0.0020 0.4903 2.5760  2041 575 0.7423 0.0013 0.3352 1.5104 0.0026 0.6821 1.2041 0.0021 0.5438 2.1296  2042 653 0.9001 0.0014 0.3938 1.7532 0.0027 0.7671 0.9186 0.0014 0.4019 2.0465  2043 553 0.7304 0.0013 0.3314 1.4873 0.0027 0.6749 1.0866 0.0020 0.4931 1.8370  2044 493 0.6578 0.0013 0.2957 1.2797 0.0026 0.5753 1.1374 0.0023 0.5113 1.8954  2045 542 0.7590 0.0014 0.3476 1.5594 0.0029 0.7141 1.2199 0.0023 0.5586 1.9958  2046 596 0.8375 0.0014 0.3621 1.6791 0.0028 0.7260 1.1027 0.0018 0.4768 2.5145  2047 569 0.6987 0.0012 0.3108 1.4471 0.0025 0.6438 1.1655 0.0021 0.5185 2.2601  2048 522 0.6951 0.0013 0.3250 1.4157 0.0027 0.6619 1.0777 0.0021 0.5039 2.0656  2049 539 0.7987 0.0015 0.3704 1.5698 0.0029 0.7280 1.2332 0.0023 0.5719 2.2375  2050 553 0.7461 0.0013 0.3703 1.5279 0.0028 0.7583 1.1182 0.0020 0.5549 1.8408  2051 556 0.8064 0.0015 0.3794 1.6042 0.0029 0.7547 0.7759 0.0014 0.3650 1.7254 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM. Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 3026 470 0.7020 0.0015 0.3422 1.4032 0.0030 0.6840 0.9439 0.0020 0.4601 2.0941  3027 560 0.6993 0.0012 0.3246 1.4095 0.0025 0.6543 0.9840 0.0018 0.4568 2.4736  3028 629 0.7754 0.0012 0.3681 1.5200 0.0024 0.7216 1.2795 0.0020 0.6075 2.6763  3029 578 NT - - NT - - 1.0106 0.0017 0.4714 2.1564  3030 612 0.7565 0.0012 0.3490 1.4998 0.0025 0.6919 1.3336 0.0022 0.6152 2.9086  3031 509 0.7999 0.0016 0.3589 1.5799 0.0031 0.7088 1.0587 0.0021 0.4750 2.1199  3032 492 0.6503 0.0013 0.3126 1.2823 0.0026 0.6164 1.0527 0.0021 0.5060 1.6005  3033 503 0.6882 0.0014 0.3137 1.4175 0.0028 0.6462 0.8402 0.0017 0.3830 2.0951  3034 583 0.7170 0.0012 0.3647 1.4015 0.0024 0.7129 1.0953 0.0019 0.5572 2.8447  3035 480 0.6766 0.0014 0.3355 1.4152 0.0029 0.7017 1.1480 0.0024 0.5692 2.2294  3036 563 0.7437 0.0013 0.3433 1.4952 0.0027 0.6903 1.2016 0.0021 0.5547 2.6152  3037 464 0.6767 0.0015 0.3025 1.3642 0.0029 0.6099 0.9638 0.0021 0.4309 1.9637  3038 576 0.7201 0.0013 0.3464 1.4413 0.0025 0.6934 1.1506 0.0020 0.5535 2.3384  3039 515 0.6946 0.0013 0.3234 1.4115 0.0027 0.6572 0.9147 0.0018 0.4259 1.7086  3040 584 0.7304 0.0013 0.3262 1.5408 0.0026 0.6882 1.0910 0.0019 0.4873 2.1297  3041 633 0.8839 0.0014 0.3892 1.8126 0.0029 0.7981 1.0632 0.0017 0.4681 2.3970  3042 523 0.6610 0.0013 0.3260 1.3353 0.0026 0.6585 1.1815 0.0023 0.5826 2.4976  3043 495 0.8462 0.0017 0.4036 1.7450 0.0035 0.8324 1.2342 0.0025 0.5887 2.4680  3044 595 0.8436 0.0014 0.3801 1.5894 0.0027 0.7161 1.1518 0.0019 0.5189 2.7763  3045 546 0.6880 0.0013 0.3073 1.4264 0.0026 0.6371 1.1350 0.0021 0.5069 2.2056  3046 637 0.7203 0.0011 0.3188 1.4963 0.0024 0.6622 1.1458 0.0018 0.5071 2.7745  3047 553 0.6789 0.0012 0.3179 1.3636 0.0025 0.6386 1.0390 0.0019 0.4866 2.0352  3048 603 0.6771 0.0011 0.3147 1.3305 0.0022 0.6184 1.0670 0.0018 0.4959 2.5289  3049 558 0.7114 0.0013 0.3397 1.3959 0.0025 0.6665 1.1725 0.0021 0.5599 2.5642  3050 589 0.9502 0.0016 0.4200 1.8274 0.0031 0.8078 1.0546 0.0018 0.4662 2.5846  3051 608 0.7796 0.0013 0.3521 1.5075 0.0025 0.6808 1.2741 0.0021 0.5754 2.2146 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT NT=NOT TAKEN: left epididymis lost during necropsy; no weight for the left epididymis or total epididymis was tak
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM. Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 4026 554 0.7099 0.0013 0.3068 1.4820 0.0027 0.6406 1.0049 0.0018 0.4343 2.3174  4027 503 0.6080 0.0012 0.2829 1.2687 0.0025 0.5903 0.9801 0.0019 0.4560 2.0843  4028 565 0.6362 0.0011 0.3085 1.3242 0.0023 0.6422 1.1984 0.0021 0.5812 2.2365  4029 564 0.6739 0.0012 0.3310 1.4150 0.0025 0.6950 1.1026 0.0020 0.5415 2.2827  4030 557 0.7389 0.0013 0.3502 1.4187 0.0025 0.6724 1.1533 0.0021 0.5466 2.3080  4031 513 0.6876 0.0013 0.3277 1.3730 0.0027 0.6543 1.4275 0.0028 0.6803 2.4848  4032 558 0.6610 0.0012 0.3010 1.3301 0.0024 0.6057 1.2606 0.0023 0.5740 2.2693  4033 561 0.7674 0.0014 0.3757 1.4691 0.0026 0.7192 1.9336 0.0034 0.9466 2.2281  4034 492 0.6866 0.0014 0.3428 1.3558 0.0028 0.6769 1.1357 0.0023 0.5670 3.2960  4035 586 0.7470 0.0013 0.3293 1.5079 0.0026 0.6647 0.7536 0.0013 0.3322 1.7678  4052 522 0.6550 0.0013 0.3079 1.3367 0.0026 0.6284 1.0195 0.0020 0.4793 1.6185  4037 498 0.6072 0.0012 0.2971 1.2009 0.0024 0.5876 0.9774 0.0020 0.4782 2.3974  4038 551 0.7624 0.0014 0.3442 1.5048 0.0027 0.6794 1.2689 0.0023 0.5729 1.9664  4039 534 0.7093 0.0013 0.3291 1.4159 0.0027 0.6569 0.8636 0.0016 0.4007 1.9148  4040 503 0.7995 0.0016 0.3866 1.6030 0.0032 0.7751 0.9294 0.0018 0.4494 2.0037  4041 562 0.6360 0.0011 0.3103 1.3183 0.0023 0.6433 0.9908 0.0018 0.4835 1.8708  4042 492 0.6517 0.0013 0.3209 1.2895 0.0026 0.6350 1.2384 0.0025 0.6098 2.5260  4043 532 0.6515 0.0012 0.3121 1.3365 0.0025 0.6403 0.9862 0.0019 0.4725 2.2523  4044 586 0.6785 0.0012 0.3303 1.3712 0.0023 0.6675 1.2708 0.0022 0.6187 2.2590  4045 590 0.8010 0.0014 0.3526 1.5660 0.0027 0.6893 1.0441 0.0018 0.4596 2.0211  4046 511 0.7240 0.0014 0.3431 1.4275 0.0028 0.6765 1.3984 0.0027 0.6627 2.6634  4047 525 0.7471 0.0014 0.3552 1.5166 0.0029 0.7210 1.1587 0.0022 0.5508 1.7321  4048 515 0.7786 0.0015 0.3572 1.4957 0.0029 0.6862 1.1645 0.0023 0.5342 1.9966  4049 459 0.6674 0.0015 0.3193 1.2819 0.0028 0.6133 1.1250 0.0025 0.5383 2.1268  4050 515 0.6757 0.0013 0.3334 1.4037 0.0027 0.6927 1.0605 0.0021 0.5233 1.7297  4051 587 0.6876 0.0012 0.3274 1.3430 0.0023 0.6394 0.9352 0.0016 0.4452 1.7378 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:39 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 1026 542 0.0525 0.0001 0.0268 1.9600 0.0036 1.0000 4.0573 0.0075 2.0701 22.1260  1027 525 0.0568 0.0001 0.0271 2.0927 0.0040 1.0000 4.1085 0.0078 1.9633 21.3042  1028 542 0.0721 0.0001 0.0353 2.0408 0.0038 1.0000 3.7434 0.0069 1.8343 17.9095  1029 564 0.0739 0.0001 0.0320 2.3129 0.0041 1.0000 3.9539 0.0070 1.7095 20.1313  1030 614 0.0814 0.0001 0.0348 2.3378 0.0038 1.0000 4.7951 0.0078 2.0511 24.1073  1031 528 0.0593 0.0001 0.0281 2.1118 0.0040 1.0000 3.7363 0.0071 1.7692 18.1974  1032 518 0.0764 0.0001 0.0354 2.1608 0.0042 1.0000 4.0704 0.0079 1.8837 20.1219  1033 751 0.0652 0.0001 0.0283 2.3068 0.0031 1.0000 5.6246 0.0075 2.4383 24.7549  1034 610 0.0786 0.0001 0.0372 2.1141 0.0035 1.0000 4.1620 0.0068 1.9687 19.4239  1035 568 0.0737 0.0001 0.0357 2.0647 0.0036 1.0000 3.7682 0.0066 1.8251 19.0380  1036 547 0.0594 0.0001 0.0272 2.1803 0.0040 1.0000 4.3142 0.0079 1.9787 18.6908  1037 576 0.0762 0.0001 0.0356 2.1382 0.0037 1.0000 4.9742 0.0086 2.3263 21.3333  1038 598 0.0698 0.0001 0.0307 2.2701 0.0038 1.0000 4.2047 0.0070 1.8522 17.9105  1039 548 0.0627 0.0001 0.0306 2.0486 0.0037 1.0000 3.7899 0.0069 1.8500 18.4642  1040 665 0.0569 0.0001 0.0270 2.1086 0.0032 1.0000 4.4344 0.0067 2.1030 24.1149  1041 564 0.0640 0.0001 0.0314 2.0397 0.0036 1.0000 3.7713 0.0067 1.8489 20.0194  1042 595 0.0639 0.0001 0.0299 2.1397 0.0036 1.0000 3.9015 0.0066 1.8234 20.3243  1043 582 0.0674 0.0001 0.0309 2.1834 0.0038 1.0000 4.1631 0.0072 1.9067 22.7770  1044 550 0.0620 0.0001 0.0294 2.1056 0.0038 1.0000 3.9725 0.0072 1.8866 19.7231  1045 451 0.0623 0.0001 0.0305 2.0454 0.0045 1.0000 3.5813 0.0079 1.7509 17.1439  1046 545 0.0652 0.0001 0.0301 2.1676 0.0040 1.0000 4.2761 0.0078 1.9727 18.6120  1047 603 0.0588 0.0001 0.0279 2.1067 0.0035 1.0000 4.6003 0.0076 2.1837 21.8246  1048 487 0.0646 0.0001 0.0318 2.0300 0.0042 1.0000 3.5655 0.0073 1.7564 15.5860  1049 556 0.0718 0.0001 0.0335 2.1462 0.0039 1.0000 4.0360 0.0073 1.8805 19.7612  1050 560 0.0714 0.0001 0.0326 2.1918 0.0039 1.0000 4.0888 0.0073 1.8655 18.7089  1051 613 0.0574 0.0001 0.0252 2.2763 0.0037 1.0000 4.5208 0.0074 1.9860 20.9055 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:40 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 2026 607 0.0589 0.0001 0.0259 2.2767 0.0038 1.0000 4.5702 0.0075 2.0074 25.5318  2027 585 0.0713 0.0001 0.0352 2.0233 0.0035 1.0000 4.8185 0.0082 2.3815 21.5290  2028 589 0.0426 0.0001 0.0196 2.1720 0.0037 1.0000 4.5067 0.0076 2.0749 20.3840  2029 645 0.0704 0.0001 0.0299 2.3532 0.0037 1.0000 5.5555 0.0086 2.3608 26.7394  2030 613 0.0677 0.0001 0.0301 2.2493 0.0037 1.0000 4.7144 0.0077 2.0959 23.4842  2031 552 0.0785 0.0001 0.0350 2.2412 0.0041 1.0000 4.3966 0.0080 1.9617 20.0163  2032 598 0.0686 0.0001 0.0298 2.2996 0.0038 1.0000 4.1701 0.0070 1.8134 21.4018  2033 671 0.0594 0.0001 0.0299 1.9852 0.0030 1.0000 3.8794 0.0058 1.9542 22.4460  2034 573 0.0594 0.0001 0.0281 2.1128 0.0037 1.0000 3.7074 0.0065 1.7547 20.3094  2035 551 0.0808 0.0001 0.0391 2.0648 0.0037 1.0000 4.2406 0.0077 2.0538 20.5866  2036 605 0.0794 0.0001 0.0364 2.1839 0.0036 1.0000 4.4923 0.0074 2.0570 21.0306  2037 558 0.0569 0.0001 0.0268 2.1228 0.0038 1.0000 4.0845 0.0073 1.9241 18.8533  2038 538 0.0634 0.0001 0.0307 2.0659 0.0038 1.0000 4.0393 0.0075 1.9552 20.3720  2039 604 0.0703 0.0001 0.0325 2.1633 0.0036 1.0000 4.8134 0.0080 2.2250 23.0134  2040 531 0.0749 0.0001 0.0348 2.1497 0.0041 1.0000 4.2977 0.0081 1.9992 21.3710  2041 575 0.0774 0.0001 0.0350 2.2143 0.0039 1.0000 4.6990 0.0082 2.1221 20.4692  2042 653 0.0683 0.0001 0.0299 2.2855 0.0035 1.0000 5.1026 0.0078 2.2326 25.3389  2043 553 0.0714 0.0001 0.0324 2.2037 0.0040 1.0000 4.1064 0.0074 1.8634 21.2498  2044 493 0.0587 0.0001 0.0264 2.2244 0.0045 1.0000 3.9720 0.0081 1.7857 19.6869  2045 542 0.0634 0.0001 0.0290 2.1838 0.0040 1.0000 4.2644 0.0079 1.9527 18.2469  2046 596 0.0931 0.0002 0.0403 2.3127 0.0039 1.0000 4.4168 0.0074 1.9098 21.9979  2047 569 0.0569 0.0001 0.0253 2.2479 0.0040 1.0000 4.0449 0.0071 1.7994 16.6868  2048 522 0.0605 0.0001 0.0283 2.1388 0.0041 1.0000 3.7223 0.0071 1.7404 17.0217  2049 539 0.0618 0.0001 0.0287 2.1564 0.0040 1.0000 4.0904 0.0076 1.8969 19.3021  2050 553 0.0454 0.0001 0.0225 2.0150 0.0036 1.0000 3.9172 0.0071 1.9440 17.7877  2051 556 0.0463 0.0001 0.0218 2.1257 0.0038 1.0000 5.2743 0.0095 2.4812 18.7173 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 3026 470 0.0651 0.0001 0.0317 2.0515 0.0044 1.0000 4.3501 0.0092 2.1204 19.4364  3027 560 0.0676 0.0001 0.0314 2.1542 0.0038 1.0000 5.5738 0.0099 2.5874 21.2057  3028 629 0.0665 0.0001 0.0316 2.1063 0.0034 1.0000 5.4729 0.0087 2.5983 25.0903  3029 578 0.0646 0.0001 0.0301 2.1439 0.0037 1.0000 4.7685 0.0083 2.2242 22.0735  3030 612 0.0689 0.0001 0.0318 2.1677 0.0035 1.0000 4.9991 0.0082 2.3062 23.3981  3031 509 0.0762 0.0001 0.0342 2.2290 0.0044 1.0000 4.6741 0.0092 2.0969 18.7364  3032 492 0.0643 0.0001 0.0309 2.0803 0.0042 1.0000 4.1657 0.0085 2.0025 17.3445  3033 503 0.0675 0.0001 0.0308 2.1937 0.0044 1.0000 4.4314 0.0088 2.0201 20.4151  3034 583 0.0677 0.0001 0.0344 1.9658 0.0034 1.0000 5.8366 0.0100 2.9691 23.1594  3035 480 0.0523 0.0001 0.0259 2.0167 0.0042 1.0000 3.9855 0.0083 1.9762 17.7456  3036 563 0.0767 0.0001 0.0354 2.1661 0.0038 1.0000 4.7159 0.0084 2.1771 20.8790  3037 464 0.0612 0.0001 0.0274 2.2367 0.0048 1.0000 4.3945 0.0095 1.9647 17.4592  3038 576 0.0603 0.0001 0.0290 2.0786 0.0036 1.0000 4.9385 0.0086 2.3759 18.2503  3039 515 0.0619 0.0001 0.0288 2.1478 0.0042 1.0000 4.2287 0.0082 1.9689 17.0769  3040 584 0.0868 0.0001 0.0388 2.2388 0.0038 1.0000 5.2370 0.0090 2.3392 26.2080  3041 633 0.0548 0.0001 0.0241 2.2712 0.0036 1.0000 5.4608 0.0086 2.4044 25.0291  3042 523 0.0701 0.0001 0.0346 2.0279 0.0039 1.0000 4.3563 0.0083 2.1482 18.7716  3043 495 0.0730 0.0001 0.0348 2.0964 0.0042 1.0000 4.7973 0.0097 2.2884 17.8664  3044 595 0.0830 0.0001 0.0374 2.2195 0.0037 1.0000 4.7853 0.0080 2.1560 21.3215  3045 546 0.0878 0.0002 0.0392 2.2390 0.0041 1.0000 4.6883 0.0086 2.0939 20.5298  3046 637 0.1033 0.0002 0.0457 2.2595 0.0035 1.0000 5.1714 0.0081 2.2887 25.3733  3047 553 0.0633 0.0001 0.0296 2.1354 0.0039 1.0000 4.5468 0.0082 2.1292 19.4227  3048 603 0.0749 0.0001 0.0348 2.1516 0.0036 1.0000 4.5839 0.0076 2.1305 21.0167  3049 558 0.0582 0.0001 0.0278 2.0943 0.0038 1.0000 4.6459 0.0083 2.2184 18.9172  3050 589 0.0623 0.0001 0.0275 2.2623 0.0038 1.0000 5.0528 0.0086 2.2335 22.3741  3051 608 0.0586 0.0001 0.0265 2.2142 0.0036 1.0000 5.2383 0.0086 2.3658 21.7969 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 4026 554 0.0683 0.0001 0.0295 2.3136 0.0042 1.0000 4.4343 0.0080 1.9166 25.2198  4027 503 0.0863 0.0002 0.0402 2.1493 0.0043 1.0000 5.2125 0.0104 2.4252 28.2262  4028 565 0.0684 0.0001 0.0332 2.0621 0.0037 1.0000 5.8276 0.0103 2.8261 26.2643  4029 564 0.0856 0.0002 0.0420 2.0361 0.0036 1.0000 4.6920 0.0083 2.3044 20.3377  4030 557 0.0673 0.0001 0.0319 2.1098 0.0038 1.0000 4.7593 0.0085 2.2558 24.1424  4031 513 0.0780 0.0002 0.0372 2.0984 0.0041 1.0000 4.6146 0.0090 2.1991 19.9476  4032 558 0.0743 0.0001 0.0338 2.1960 0.0039 1.0000 5.0668 0.0091 2.3073 26.9067  4033 561 0.0702 0.0001 0.0344 2.0426 0.0036 1.0000 5.6209 0.0100 2.7518 21.0550  4034 492 0.0621 0.0001 0.0310 2.0030 0.0041 1.0000 4.6279 0.0094 2.3105 19.7124  4035 586 0.0646 0.0001 0.0285 2.2685 0.0039 1.0000 4.6238 0.0079 2.0383 19.7820  4052 522 0.0759 0.0001 0.0357 2.1270 0.0041 1.0000 4.4327 0.0085 2.0840 18.4764  4037 498 0.0524 0.0001 0.0256 2.0438 0.0041 1.0000 4.6940 0.0094 2.2967 20.4451  4038 551 0.0822 0.0001 0.0371 2.2150 0.0040 1.0000 5.5779 0.0101 2.5182 20.4384  4039 534 0.0906 0.0002 0.0420 2.1553 0.0040 1.0000 5.3236 0.0100 2.4700 23.1827  4040 503 0.0695 0.0001 0.0336 2.0682 0.0041 1.0000 5.0749 0.0101 2.4538 25.8386  4041 562 0.0754 0.0001 0.0368 2.0494 0.0036 1.0000 5.0627 0.0090 2.4703 20.7616  4042 492 0.0706 0.0001 0.0348 2.0308 0.0041 1.0000 4.0653 0.0083 2.0018 19.9851  4043 532 0.0629 0.0001 0.0301 2.0874 0.0039 1.0000 4.5341 0.0085 2.1721 23.7428  4044 586 0.0800 0.0001 0.0389 2.0541 0.0035 1.0000 5.5625 0.0095 2.7080 22.5998  4045 590 0.0641 0.0001 0.0282 2.2720 0.0038 1.0000 4.8709 0.0083 2.1439 22.7772  4046 511 0.0657 0.0001 0.0311 2.1101 0.0041 1.0000 4.9233 0.0096 2.3332 21.4057  4047 525 0.0911 0.0002 0.0433 2.1036 0.0040 1.0000 4.7923 0.0091 2.2781 21.4898  4048 515 0.0663 0.0001 0.0304 2.1798 0.0042 1.0000 4.7179 0.0092 2.1644 21.5532  4049 459 0.0752 0.0002 0.0360 2.0901 0.0046 1.0000 4.2238 0.0092 2.0209 17.4827  4050 515 0.0610 0.0001 0.0301 2.0264 0.0039 1.0000 4.9284 0.0096 2.4321 17.7900  4051 587 0.0542 0.0001 0.0258 2.1005 0.0036 1.0000 4.5607 0.0078 2.1712 20.4977 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:39 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 1026 542 1.9402 0.0036 0.9899 0.0099 0.0000 0.0051 0.9620 0.0018 0.4908  1027 525 1.6292 0.0031 0.7785 0.0132 0.0000 0.0063 0.8048 0.0015 0.3846  1028 542 1.7804 0.0033 0.8724 0.0135 0.0000 0.0066 0.6995 0.0013 0.3428  1029 564 2.0914 0.0037 0.9042 0.0119 0.0000 0.0051 0.9429 0.0017 0.4077  1030 614 2.3016 0.0038 0.9845 0.0154 0.0000 0.0066 1.1808 0.0019 0.5051  1031 528 1.6212 0.0031 0.7677 0.0110 0.0000 0.0052 0.7903 0.0015 0.3742  1032 518 1.8551 0.0036 0.8585 0.0137 0.0000 0.0063 0.9342 0.0018 0.4323  1033 751 2.4965 0.0033 1.0822 0.0166 0.0000 0.0072 1.0930 0.0015 0.4738  1034 610 2.1346 0.0035 1.0097 0.0126 0.0000 0.0060 0.7984 0.0013 0.3777  1035 568 2.1062 0.0037 1.0201 0.0113 0.0000 0.0055 0.7272 0.0013 0.3522  1036 547 2.2192 0.0041 1.0178 0.0134 0.0000 0.0061 0.8791 0.0016 0.4032  1037 576 2.0988 0.0036 0.9816 0.0121 0.0000 0.0057 0.9870 0.0017 0.4616  1038 598 2.0166 0.0034 0.8883 0.0127 0.0000 0.0056 1.1235 0.0019 0.4949  1039 548 1.7335 0.0032 0.8462 0.0100 0.0000 0.0049 0.6377 0.0012 0.3113  1040 665 2.0142 0.0030 0.9552 0.0135 0.0000 0.0064 1.1035 0.0017 0.5233  1041 564 1.8671 0.0033 0.9154 0.0113 0.0000 0.0055 0.9211 0.0016 0.4516  1042 595 1.9357 0.0033 0.9047 0.0147 0.0000 0.0069 0.9742 0.0016 0.4553  1043 582 1.9858 0.0034 0.9095 0.0115 0.0000 0.0053 0.8861 0.0015 0.4058  1044 550 2.1179 0.0039 1.0058 0.0136 0.0000 0.0065 0.8627 0.0016 0.4097  1045 451 1.6045 0.0036 0.7844 0.0101 0.0000 0.0049 0.6563 0.0015 0.3209  1046 545 2.1282 0.0039 0.9818 0.0132 0.0000 0.0061 0.8278 0.0015 0.3819  1047 603 1.8697 0.0031 0.8875 0.0140 0.0000 0.0066 0.9746 0.0016 0.4626  1048 487 1.6858 0.0035 0.8304 0.0107 0.0000 0.0053 0.7909 0.0016 0.3896  1049 556 2.0741 0.0037 0.9664 0.0123 0.0000 0.0057 0.9373 0.0017 0.4367  1050 560 1.9825 0.0035 0.9045 0.0111 0.0000 0.0051 0.7880 0.0014 0.3595  1051 613 1.9922 0.0032 0.8752 0.0142 0.0000 0.0062 1.0652 0.0017 0.4680 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:40 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 2026 607 2.0061 0.0033 0.8811 0.0117 0.0000 0.0051 0.8287 0.0014 0.3640  2027 585 1.9720 0.0034 0.9746 0.0140 0.0000 0.0069 0.7211 0.0012 0.3564  2028 589 1.8646 0.0032 0.8585 0.0139 0.0000 0.0064 1.0093 0.0017 0.4647  2029 645 2.5436 0.0039 1.0809 0.0098 0.0000 0.0042 1.0029 0.0016 0.4262  2030 613 2.1366 0.0035 0.9499 0.0130 0.0000 0.0058 0.9406 0.0015 0.4182  2031 552 2.7423 0.0050 1.2236 0.0136 0.0000 0.0061 0.9004 0.0016 0.4017  2032 598 1.9917 0.0033 0.8661 0.0142 0.0000 0.0062 0.6750 0.0011 0.2935  2033 671 2.0950 0.0031 1.0553 0.0127 0.0000 0.0064 0.8326 0.0012 0.4194  2034 573 1.8724 0.0033 0.8862 0.0136 0.0000 0.0064 0.6829 0.0012 0.3232  2035 551 1.8542 0.0034 0.8980 0.0133 0.0000 0.0064 0.8365 0.0015 0.4051  2036 605 2.0372 0.0034 0.9328 0.0134 0.0000 0.0061 1.0287 0.0017 0.4710  2037 558 1.8588 0.0033 0.8756 0.0119 0.0000 0.0056 0.9062 0.0016 0.4269  2038 538 1.7794 0.0033 0.8613 0.0124 0.0000 0.0060 0.7929 0.0015 0.3838  2039 604 2.1551 0.0036 0.9962 0.0120 0.0000 0.0055 0.7501 0.0012 0.3467  2040 531 2.0996 0.0040 0.9767 0.0149 0.0000 0.0069 0.8319 0.0016 0.3870  2041 575 1.9061 0.0033 0.8608 0.0161 0.0000 0.0073 0.9873 0.0017 0.4459  2042 653 1.9567 0.0030 0.8561 0.0145 0.0000 0.0063 0.9523 0.0015 0.4167  2043 553 1.9619 0.0036 0.8903 0.0119 0.0000 0.0054 1.0128 0.0018 0.4596  2044 493 2.0369 0.0041 0.9157 0.0085 0.0000 0.0038 0.7496 0.0015 0.3370  2045 542 1.9541 0.0036 0.8948 0.0112 0.0000 0.0051 0.7655 0.0014 0.3505  2046 596 2.4195 0.0041 1.0462 0.0134 0.0000 0.0058 0.9032 0.0015 0.3905  2047 569 1.8637 0.0033 0.8291 0.0126 0.0000 0.0056 0.9421 0.0017 0.4191  2048 522 1.8195 0.0035 0.8507 0.0115 0.0000 0.0054 0.6863 0.0013 0.3209  2049 539 1.8527 0.0034 0.8592 0.0114 0.0000 0.0053 0.6996 0.0013 0.3244  2050 553 1.8160 0.0033 0.9012 0.0143 0.0000 0.0071 0.7683 0.0014 0.3813  2051 556 1.8129 0.0033 0.8528 0.0151 0.0000 0.0071 0.8214 0.0015 0.3864 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 3026 470 1.7183 0.0037 0.8376 0.0110 0.0000 0.0054 0.6711 0.0014 0.3271  3027 560 1.7635 0.0031 0.8186 0.0088 0.0000 0.0041 0.8236 0.0015 0.3823  3028 629 1.9330 0.0031 0.9177 0.0164 0.0000 0.0078 0.7990 0.0013 0.3793  3029 578 2.1961 0.0038 1.0243 0.0048 0.0000 0.0022 0.7956 0.0014 0.3711  3030 612 1.7779 0.0029 0.8202 0.0148 0.0000 0.0068 0.9166 0.0015 0.4228  3031 509 1.8567 0.0036 0.8330 0.0135 0.0000 0.0061 0.8177 0.0016 0.3668  3032 492 1.7313 0.0035 0.8322 0.0113 0.0000 0.0054 0.8392 0.0017 0.4034  3033 503 1.7909 0.0036 0.8164 0.0100 0.0000 0.0046 0.5691 0.0011 0.2594  3034 583 1.7033 0.0029 0.8665 0.0160 0.0000 0.0081 0.7715 0.0013 0.3925  3035 480 1.6747 0.0035 0.8304 0.0091 0.0000 0.0045 0.7932 0.0017 0.3933  3036 563 1.8271 0.0032 0.8435 0.0130 0.0000 0.0060 0.7930 0.0014 0.3661  3037 464 1.9074 0.0041 0.8528 0.0110 0.0000 0.0049 0.7645 0.0016 0.3418  3038 576 1.6950 0.0029 0.8155 0.0159 0.0000 0.0076 0.9165 0.0016 0.4409  3039 515 1.8226 0.0035 0.8486 0.0116 0.0000 0.0054 0.8370 0.0016 0.3897  3040 584 2.2045 0.0038 0.9847 0.0138 0.0000 0.0062 0.9701 0.0017 0.4333  3041 633 2.1469 0.0034 0.9453 0.0132 0.0000 0.0058 1.1318 0.0018 0.4983  3042 523 1.7223 0.0033 0.8493 0.0121 0.0000 0.0060 0.7340 0.0014 0.3620  3043 495 1.7071 0.0034 0.8143 0.0118 0.0000 0.0056 0.5375 0.0011 0.2564  3044 595 2.1811 0.0037 0.9827 0.0129 0.0000 0.0058 0.8552 0.0014 0.3853  3045 546 1.7942 0.0033 0.8013 0.0094 0.0000 0.0042 0.6157 0.0011 0.2750  3046 637 2.1426 0.0034 0.9483 0.0126 0.0000 0.0056 0.9975 0.0016 0.4415  3047 553 2.0711 0.0037 0.9699 0.0097 0.0000 0.0045 0.7999 0.0014 0.3746  3048 603 1.9389 0.0032 0.9011 0.0135 0.0000 0.0063 0.7877 0.0013 0.3661  3049 558 1.8626 0.0033 0.8894 0.0099 0.0000 0.0047 0.8947 0.0016 0.4272  3050 589 2.2451 0.0038 0.9924 0.0145 0.0000 0.0064 0.9580 0.0016 0.4235  3051 608 1.8680 0.0031 0.8436 0.0112 0.0000 0.0051 0.7961 0.0013 0.3595 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:41 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS MALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 4026 554 1.7862 0.0032 0.7720 0.0110 0.0000 0.0048 0.8137 0.0015 0.3517  4027 503 1.9986 0.0040 0.9299 0.0147 0.0000 0.0068 0.6680 0.0013 0.3108  4028 565 1.8421 0.0033 0.8933 0.0126 0.0000 0.0061 0.7723 0.0014 0.3745  4029 564 2.1121 0.0037 1.0373 0.0159 0.0000 0.0078 0.8917 0.0016 0.4379  4030 557 2.2741 0.0041 1.0779 0.0131 0.0000 0.0062 0.8875 0.0016 0.4207  4031 513 1.7523 0.0034 0.8351 0.0127 0.0000 0.0061 0.6539 0.0013 0.3116  4032 558 1.6215 0.0029 0.7384 0.0145 0.0000 0.0066 0.7545 0.0014 0.3436  4033 561 1.7530 0.0031 0.8582 0.0164 0.0000 0.0080 0.6664 0.0012 0.3263  4034 492 1.6287 0.0033 0.8131 0.0129 0.0000 0.0064 0.8213 0.0017 0.4100  4035 586 1.9064 0.0033 0.8404 0.0140 0.0000 0.0062 0.8296 0.0014 0.3657  4052 522 1.9100 0.0037 0.8980 0.0129 0.0000 0.0061 0.6865 0.0013 0.3228  4037 498 1.8465 0.0037 0.9035 0.0105 0.0000 0.0051 0.7646 0.0015 0.3741  4038 551 1.8696 0.0034 0.8441 0.0142 0.0000 0.0064 0.7459 0.0014 0.3367  4039 534 1.8699 0.0035 0.8676 0.0112 0.0000 0.0052 0.8789 0.0016 0.4078  4040 503 1.7503 0.0035 0.8463 0.0143 0.0000 0.0069 0.7595 0.0015 0.3672  4041 562 1.8181 0.0032 0.8871 0.0124 0.0000 0.0061 0.6229 0.0011 0.3039  4042 492 1.6220 0.0033 0.7987 0.0116 0.0000 0.0057 0.8098 0.0016 0.3988  4043 532 2.0453 0.0038 0.9798 0.0111 0.0000 0.0053 0.7608 0.0014 0.3645  4044 586 2.3214 0.0040 1.1301 0.0162 0.0000 0.0079 0.6029 0.0010 0.2935  4045 590 1.9521 0.0033 0.8592 0.0133 0.0000 0.0059 0.8167 0.0014 0.3595  4046 511 1.7747 0.0035 0.8411 0.0153 0.0000 0.0073 0.7520 0.0015 0.3564  4047 525 1.8851 0.0036 0.8961 0.0110 0.0000 0.0052 0.8364 0.0016 0.3976  4048 515 1.7573 0.0034 0.8062 0.0104 0.0000 0.0048 0.6338 0.0012 0.2908  4049 459 1.6718 0.0036 0.7999 0.0092 0.0000 0.0044 0.5985 0.0013 0.2863  4050 515 1.9732 0.0038 0.9737 0.0126 0.0000 0.0062 0.7831 0.0015 0.3864  4051 587 1.7222 0.0029 0.8199 0.0117 0.0000 0.0056 0.8046 0.0014 0.3831 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:43 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ OVARY RIGHT OVARY LEFT OVARIES UTER Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 1526 319 0.0361 0.0001 0.0191 0.0554 0.0002 0.0294 0.0893 0.0003 0.0474 0.5245  1528 350 0.0509 0.0001 0.0230 0.0560 0.0002 0.0253 0.1080 0.0003 0.0488 0.6691  1529 PNMD 358 0.0580 0.0002 0.0262 0.0535 0.0001 0.0242 0.1069 0.0003 0.0483 0.7184  1530 341 0.0594 0.0002 0.0290 0.0619 0.0002 0.0302 0.1212 0.0004 0.0592 0.5966  1531 347 0.0817 0.0002 0.0384 0.0623 0.0002 0.0293 0.1399 0.0004 0.0657 0.5760  1533 339 0.0714 0.0002 0.0345 0.1611 0.0005 0.0777 0.2281 0.0007 0.1101 0.5591  1534 317 0.0513 0.0002 0.0279 0.0568 0.0002 0.0309 0.1071 0.0003 0.0583 0.9397  1536 322 0.0696 0.0002 0.0383 0.0590 0.0002 0.0325 0.1281 0.0004 0.0705 0.7344  1537 302 0.0908 0.0003 0.0470 0.0538 0.0002 0.0279 0.1361 0.0005 0.0705 0.5009  1538 320 0.0342 0.0001 0.0184 0.0367 0.0001 0.0198 0.0693 0.0002 0.0373 1.0990  1539 330 0.0779 0.0002 0.0381 0.0708 0.0002 0.0346 0.1469 0.0004 0.0718 0.7145  1540 320 0.0638 0.0002 0.0331 0.0555 0.0002 0.0288 0.1171 0.0004 0.0608 1.0916  1542 347 0.0585 0.0002 0.0302 0.0834 0.0002 0.0431 0.1387 0.0004 0.0716 0.9121  1543 320 0.0643 0.0002 0.0336 0.0647 0.0002 0.0338 0.1280 0.0004 0.0669 0.9897  1544 360 0.0545 0.0002 0.0276 0.0513 0.0001 0.0260 0.1042 0.0003 0.0528 0.5743  1545 381 0.0594 0.0002 0.0282 0.0565 0.0001 0.0268 0.1152 0.0003 0.0546 0.5912  1546 299 0.0537 0.0002 0.0275 0.0383 0.0001 0.0196 0.0892 0.0003 0.0456 0.5430  1547 309 0.0686 0.0002 0.0379 0.0480 0.0002 0.0265 0.1160 0.0004 0.0641 0.6849  1549 332 0.0800 0.0002 0.0399 0.0759 0.0002 0.0379 0.1517 0.0005 0.0757 0.5715  1551 286 0.0638 0.0002 0.0345 0.0550 0.0002 0.0297 0.1169 0.0004 0.0632 0.7763 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:43 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ OVARY RIGHT OVARY LEFT OVARIES UTER Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 2526 320 0.0607 0.0002 0.0309 0.0591 0.0002 0.0301 0.1159 0.0004 0.0590 0.6193  2528 315 0.0547 0.0002 0.0292 0.0657 0.0002 0.0351 0.1041 0.0003 0.0557 0.5201  2529 348 0.0565 0.0002 0.0272 0.0501 0.0001 0.0242 0.1075 0.0003 0.0518 0.4817  2530 371 0.0485 0.0001 0.0228 0.0455 0.0001 0.0214 0.0990 0.0003 0.0465 0.6215  2531 310 0.0526 0.0002 0.0277 0.0462 0.0001 0.0244 0.0964 0.0003 0.0508 0.9657  2533 334 0.0699 0.0002 0.0349 0.0600 0.0002 0.0300 0.1267 0.0004 0.0633 0.5117  2534 295 0.0737 0.0002 0.0369 0.0702 0.0002 0.0351 0.1439 0.0005 0.0720 0.6088  2535 349 0.0561 0.0002 0.0305 0.0444 0.0001 0.0242 0.1007 0.0003 0.0548 0.4523  2536 338 0.0701 0.0002 0.0341 0.0548 0.0002 0.0267 0.1260 0.0004 0.0613 0.4528  2537 308 0.0649 0.0002 0.0345 0.0483 0.0002 0.0257 0.1116 0.0004 0.0593 0.7499  2539 344 0.0572 0.0002 0.0304 0.0563 0.0002 0.0299 0.1120 0.0003 0.0596 0.4681  2540 316 0.0497 0.0002 0.0249 0.0416 0.0001 0.0208 0.0907 0.0003 0.0454 0.5177  2541 392 0.0858 0.0002 0.0446 0.0505 0.0001 0.0263 0.1338 0.0003 0.0696 0.5874  2543 343 0.0821 0.0002 0.0411 0.0763 0.0002 0.0382 0.1554 0.0005 0.0777 0.5691  2544 337 0.0576 0.0002 0.0279 0.0445 0.0001 0.0216 0.1016 0.0003 0.0493 1.2428  2545 319 0.0877 0.0003 0.0436 0.0829 0.0003 0.0412 0.1700 0.0005 0.0846 0.7208  2546 292 0.0513 0.0002 0.0259 0.0627 0.0002 0.0317 0.1144 0.0004 0.0578 0.9454  2547 286 0.0301 0.0001 0.0159 0.0332 0.0001 0.0175 0.0663 0.0002 0.0350 0.5510  2548 342 0.0517 0.0002 0.0263 0.0545 0.0002 0.0278 0.1049 0.0003 0.0534 0.5308  2550 325 0.0663 0.0002 0.0344 0.0574 0.0002 0.0298 0.1239 0.0004 0.0643 0.6278  2551 333 0.0616 0.0002 0.0303 0.0554 0.0002 0.0272 0.1154 0.0003 0.0567 0.7515 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ OVARY RIGHT OVARY LEFT OVARIES UTER Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 3527 355 0.0911 0.0003 0.0432 0.0890 0.0003 0.0422 0.1780 0.0005 0.0843 0.6203  3528 307 0.0390 0.0001 0.0206 0.0435 0.0001 0.0230 0.0830 0.0003 0.0439 0.5311  3529 305 0.0563 0.0002 0.0291 0.0478 0.0002 0.0247 0.1057 0.0003 0.0547 0.7288  3530 325 0.0595 0.0002 0.0310 0.0625 0.0002 0.0325 0.1222 0.0004 0.0636 0.4169  3531 318 0.0736 0.0002 0.0393 0.0568 0.0002 0.0303 0.1295 0.0004 0.0692 0.5599  3532 362 0.0570 0.0002 0.0292 0.0657 0.0002 0.0337 0.1198 0.0003 0.0614 0.4499  3533 357 0.0774 0.0002 0.0390 0.0872 0.0002 0.0439 0.1658 0.0005 0.0835 0.4130  3534 313 0.0882 0.0003 0.0458 0.0714 0.0002 0.0370 0.1569 0.0005 0.0814 1.0475  3535 345 0.0462 0.0001 0.0236 0.0489 0.0001 0.0250 0.0961 0.0003 0.0492 0.4499  3537 312 0.0592 0.0002 0.0295 0.0434 0.0001 0.0217 0.1032 0.0003 0.0515 0.5341  3540 321 0.0642 0.0002 0.0319 0.0571 0.0002 0.0283 0.1228 0.0004 0.0609 0.6667  3541 326 0.0673 0.0002 0.0369 0.0570 0.0002 0.0312 0.1224 0.0004 0.0671 0.4490  3542 355 0.0632 0.0002 0.0299 0.0494 0.0001 0.0234 0.1117 0.0003 0.0529 0.6632  3543 310 0.0534 0.0002 0.0276 0.0545 0.0002 0.0282 0.1065 0.0003 0.0551 0.8786  3544 304 0.0585 0.0002 0.0293 0.0434 0.0001 0.0218 0.1017 0.0003 0.0510 1.0545  3545 326 0.0556 0.0002 0.0295 0.0557 0.0002 0.0295 0.1099 0.0003 0.0582 0.5160  3546 345 0.0632 0.0002 0.0321 0.0485 0.0001 0.0246 0.1136 0.0003 0.0577 0.5364  3547 324 0.0651 0.0002 0.0337 0.0742 0.0002 0.0384 0.1366 0.0004 0.0707 0.5587  3549 329 0.0731 0.0002 0.0354 0.0742 0.0002 0.0360 0.1459 0.0004 0.0707 0.6046  3551 337 0.0688 0.0002 0.0344 0.0768 0.0002 0.0384 0.1452 0.0004 0.0725 0.7778 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ OVARY RIGHT OVARY LEFT OVARIES UTER Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 4526 292 0.0414 0.0001 0.0201 0.0488 0.0002 0.0237 0.0898 0.0003 0.0436 0.4836  4527 348 0.0604 0.0002 0.0280 0.0636 0.0002 0.0295 0.1229 0.0004 0.0570 0.6306  4528 336 0.0589 0.0002 0.0297 0.0402 0.0001 0.0203 0.0991 0.0003 0.0500 0.4494  4529 324 0.0732 0.0002 0.0374 0.0665 0.0002 0.0340 0.1375 0.0004 0.0703 0.9971  4531 318 0.0479 0.0002 0.0254 0.0408 0.0001 0.0216 0.0862 0.0003 0.0457 0.4396  4532 321 0.0378 0.0001 0.0186 0.0196 0.0001 0.0096 0.1027 0.0003 0.0504 0.5414  4533 359 0.0436 0.0001 0.0225 0.0517 0.0001 0.0267 0.0968 0.0003 0.0499 0.5021  4534 330 0.0438 0.0001 0.0225 0.0646 0.0002 0.0332 0.1080 0.0003 0.0555 1.0670  4535 277 0.0450 0.0002 0.0233 0.0483 0.0002 0.0250 0.0864 0.0003 0.0447 0.7424  4536 321 0.0799 0.0002 0.0381 0.0749 0.0002 0.0357 0.1511 0.0005 0.0720 0.5337  4537 361 0.0521 0.0001 0.0268 0.0714 0.0002 0.0367 0.1265 0.0004 0.0651 0.4286  4538 291 0.0567 0.0002 0.0291 0.0532 0.0002 0.0273 0.1098 0.0004 0.0564 0.9779  4540 304 0.0369 0.0001 0.0194 0.0528 0.0002 0.0277 0.0884 0.0003 0.0465 0.5491  4542 314 0.0577 0.0002 0.0311 0.0552 0.0002 0.0298 0.1094 0.0003 0.0590 0.4888  4543 350 0.0779 0.0002 0.0412 0.0502 0.0001 0.0265 0.1266 0.0004 0.0669 0.6563  4544 303 0.0483 0.0002 0.0246 0.0586 0.0002 0.0298 0.1044 0.0003 0.0531 0.9878  4545 322 0.0621 0.0002 0.0306 0.0483 0.0002 0.0238 0.1178 0.0004 0.0581 0.8326  4546 333 0.0565 0.0002 0.0271 0.0590 0.0002 0.0283 0.1119 0.0003 0.0536 0.7698  4547 PNMD 325 0.0462 0.0001 0.0244 0.0638 0.0002 0.0337 0.1107 0.0003 0.0585 1.1007  4548 349 0.0510 0.0001 0.0266 0.0648 0.0002 0.0338 0.1151 0.0003 0.0600 0.4625  4550 359 0.0435 0.0001 0.0211 0.0505 0.0001 0.0244 0.1041 0.0003 0.0504 0.6736  4551 317 0.0622 0.0002 0.0342 0.0390 0.0001 0.0215 0.1084 0.0003 0.0596 0.6258 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 1526 319 0.0797 0.0002 0.0423 1.8855 0.0059 1.0000 2.5994 0.0081 1.3786 15.9652  1528 350 0.0892 0.0003 0.0403 2.2149 0.0063 1.0000 3.0228 0.0086 1.3648 15.5199  1529 PNMD 358 0.0712 0.0002 0.0322 2.2118 0.0062 1.0000 2.9845 0.0083 1.3494 16.6851  1530 341 0.0858 0.0003 0.0419 2.0481 0.0060 1.0000 3.1346 0.0092 1.5305 17.1079  1531 347 0.0694 0.0002 0.0326 2.1290 0.0061 1.0000 2.9063 0.0084 1.3651 13.8805  1533 339 0.0805 0.0002 0.0388 2.0725 0.0061 1.0000 2.8494 0.0084 1.3749 18.0052  1534 317 0.0836 0.0003 0.0455 1.8375 0.0058 1.0000 2.7259 0.0086 1.4835 14.3507  1536 322 0.0844 0.0003 0.0465 1.8161 0.0056 1.0000 2.6384 0.0082 1.4528 13.9716  1537 302 0.0989 0.0003 0.0512 1.9305 0.0064 1.0000 2.8796 0.0095 1.4916 12.8743  1538 320 0.0799 0.0002 0.0430 1.8577 0.0058 1.0000 2.5295 0.0079 1.3616 15.2784  1539 330 0.0797 0.0002 0.0390 2.0452 0.0062 1.0000 2.8847 0.0088 1.4105 14.7976  1540 320 0.0935 0.0003 0.0486 1.9252 0.0060 1.0000 2.7780 0.0087 1.4430 15.0931  1542 347 0.0961 0.0003 0.0496 1.9372 0.0056 1.0000 14.4597 0.0417 7.4642 16.8785  1543 320 0.0834 0.0003 0.0436 1.9136 0.0060 1.0000 2.6349 0.0082 1.3769 14.9966  1544 360 0.0895 0.0002 0.0454 1.9723 0.0055 1.0000 2.8769 0.0080 1.4587 17.8918  1545 381 0.0784 0.0002 0.0372 2.1095 0.0055 1.0000 2.8856 0.0076 1.3679 18.2400  1546 299 0.0790 0.0003 0.0404 1.9550 0.0065 1.0000 2.2913 0.0077 1.1720 13.0830  1547 309 0.0793 0.0003 0.0438 1.8102 0.0059 1.0000 2.4128 0.0078 1.3329 13.9238  1549 332 0.0811 0.0002 0.0405 2.0031 0.0060 1.0000 2.9424 0.0089 1.4689 14.3955  1551 286 0.0819 0.0003 0.0443 1.8496 0.0065 1.0000 2.4848 0.0087 1.3434 11.9407 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 2526 320 0.0923 0.0003 0.0470 1.9648 0.0061 1.0000 2.5608 0.0080 1.3033 14.8140  2528 315 0.0665 0.0002 0.0356 1.8706 0.0059 1.0000 2.6440 0.0084 1.4135 15.0611  2529 348 0.0892 0.0003 0.0430 2.0742 0.0060 1.0000 3.1762 0.0091 1.5313 17.9404  2530 371 0.0764 0.0002 0.0359 2.1273 0.0057 1.0000 2.6547 0.0071 1.2479 15.5799  2531 310 0.0710 0.0002 0.0374 1.8959 0.0061 1.0000 2.5763 0.0083 1.3589 14.4542  2533 334 0.0734 0.0002 0.0367 2.0013 0.0060 1.0000 2.5590 0.0077 1.2787 15.1291  2534 295 0.0945 0.0003 0.0473 1.9976 0.0068 1.0000 3.1052 0.0105 1.5545 13.0412  2535 349 0.0852 0.0002 0.0464 1.8378 0.0053 1.0000 2.9100 0.0083 1.5834 17.2314  2536 338 0.0683 0.0002 0.0332 2.0552 0.0061 1.0000 3.1500 0.0093 1.5327 15.8841  2537 308 0.0735 0.0002 0.0391 1.8813 0.0061 1.0000 2.5923 0.0084 1.3779 14.1336  2539 344 0.0870 0.0003 0.0463 1.8800 0.0055 1.0000 3.0654 0.0089 1.6305 16.7921  2540 316 0.0605 0.0002 0.0303 1.9997 0.0063 1.0000 2.4494 0.0078 1.2249 14.1950  2541 392 0.0843 0.0002 0.0438 1.9229 0.0049 1.0000 3.3702 0.0086 1.7527 20.4544  2543 343 0.0847 0.0002 0.0424 1.9989 0.0058 1.0000 3.1425 0.0092 1.5721 17.1332  2544 337 0.0839 0.0002 0.0407 2.0620 0.0061 1.0000 3.0825 0.0091 1.4949 13.3880  2545 319 0.1196 0.0004 0.0595 2.0102 0.0063 1.0000 2.9113 0.0091 1.4483 16.4452  2546 292 0.0807 0.0003 0.0408 1.9798 0.0068 1.0000 2.4123 0.0083 1.2185 13.8196  2547 286 0.0618 0.0002 0.0327 1.8924 0.0066 1.0000 2.0980 0.0073 1.1086 11.6273  2548 342 0.0752 0.0002 0.0383 1.9631 0.0057 1.0000 2.8647 0.0084 1.4593 18.7377  2550 325 0.0727 0.0002 0.0377 1.9280 0.0059 1.0000 2.6098 0.0080 1.3536 15.4699  2551 333 0.0811 0.0002 0.0399 2.0344 0.0061 1.0000 2.9879 0.0090 1.4687 19.4564 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 3527 355 0.0877 0.0002 0.0416 2.1104 0.0059 1.0000 2.9815 0.0084 1.4128 16.2401  3528 307 0.0634 0.0002 0.0335 1.8898 0.0061 1.0000 2.5007 0.0081 1.3233 12.8494  3529 305 0.0810 0.0003 0.0419 1.9321 0.0063 1.0000 3.0248 0.0099 1.5656 15.2935  3530 325 0.0662 0.0002 0.0344 1.9218 0.0059 1.0000 2.9210 0.0090 1.5199 15.4342  3531 318 0.0673 0.0002 0.0360 1.8717 0.0059 1.0000 2.6510 0.0083 1.4164 14.2479  3532 362 0.0737 0.0002 0.0378 1.9498 0.0054 1.0000 2.8674 0.0079 1.4706 16.2160  3533 357 0.0970 0.0003 0.0488 1.9864 0.0056 1.0000 2.8448 0.0080 1.4321 18.1003  3534 313 0.1018 0.0003 0.0528 1.9276 0.0062 1.0000 2.9026 0.0093 1.5058 13.3284  3535 345 0.0695 0.0002 0.0356 1.9547 0.0057 1.0000 2.7409 0.0080 1.4022 16.4886  3537 312 0.0793 0.0003 0.0396 2.0035 0.0064 1.0000 2.5365 0.0081 1.2660 14.4783  3540 321 0.0976 0.0003 0.0484 2.0154 0.0063 1.0000 2.7446 0.0085 1.3618 15.0811  3541 326 0.0714 0.0002 0.0391 1.8242 0.0056 1.0000 2.8925 0.0089 1.5856 15.3179  3542 355 0.0861 0.0002 0.0407 2.1134 0.0059 1.0000 2.9229 0.0082 1.3830 16.3857  3543 310 0.0979 0.0003 0.0507 1.9318 0.0062 1.0000 2.8065 0.0091 1.4528 12.0504  3544 304 0.0728 0.0002 0.0365 1.9951 0.0066 1.0000 3.0668 0.0101 1.5372 15.1003  3545 326 0.0887 0.0003 0.0470 1.8871 0.0058 1.0000 2.7984 0.0086 1.4829 15.8975  3546 345 0.0674 0.0002 0.0342 1.9679 0.0057 1.0000 2.9546 0.0086 1.5014 19.2074  3547 324 0.0911 0.0003 0.0471 1.9327 0.0060 1.0000 2.8802 0.0089 1.4902 16.9666  3549 329 0.0779 0.0002 0.0378 2.0627 0.0063 1.0000 2.9898 0.0091 1.4495 17.0718  3551 337 0.0806 0.0002 0.0402 2.0026 0.0059 1.0000 3.0820 0.0091 1.5390 15.7045 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ ADRENALS BRAIN KIDNEYS LIVE Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------- Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight  g g g g g ------------------------------------------------------------------------------------------------------------------ 4526 292 0.0779 0.0003 0.0379 2.0574 0.0070 1.0000 2.4938 0.0085 1.2121 13.0099  4527 348 0.0915 0.0003 0.0424 2.1576 0.0062 1.0000 3.3619 0.0097 1.5582 18.3593  4528 336 0.0731 0.0002 0.0369 1.9822 0.0059 1.0000 2.8423 0.0085 1.4339 15.7148  4529 324 0.0696 0.0002 0.0356 1.9550 0.0060 1.0000 3.0380 0.0094 1.5540 15.9810  4531 318 0.0827 0.0003 0.0438 1.8867 0.0059 1.0000 2.5675 0.0081 1.3608 15.5333  4532 321 0.0707 0.0002 0.0347 2.0364 0.0064 1.0000 2.6582 0.0083 1.3053 15.7336  4533 359 0.0853 0.0002 0.0440 1.9383 0.0054 1.0000 3.0342 0.0085 1.5654 18.3623  4534 330 0.0833 0.0003 0.0428 1.9477 0.0059 1.0000 2.9925 0.0091 1.5364 15.8291  4535 277 0.0756 0.0003 0.0391 1.9333 0.0070 1.0000 2.7033 0.0098 1.3983 12.9745  4536 321 0.0851 0.0003 0.0405 2.0993 0.0065 1.0000 2.6610 0.0083 1.2676 19.1754  4537 361 0.1116 0.0003 0.0574 1.9440 0.0054 1.0000 3.1030 0.0086 1.5962 19.2288  4538 291 0.0955 0.0003 0.0490 1.9472 0.0067 1.0000 2.8642 0.0098 1.4709 13.3085  4540 304 0.0671 0.0002 0.0353 1.9031 0.0063 1.0000 2.6022 0.0086 1.3673 14.4358  4542 314 0.5661 0.0018 0.3052 1.8549 0.0059 1.0000 2.9713 0.0095 1.6019 15.6630  4543 350 0.0820 0.0002 0.0434 1.8913 0.0054 1.0000 2.8672 0.0082 1.5160 18.8812  4544 303 0.0812 0.0003 0.0413 1.9652 0.0065 1.0000 2.9504 0.0097 1.5013 15.0090  4545 322 0.0730 0.0002 0.0360 2.0279 0.0063 1.0000 3.1900 0.0099 1.5731 15.5356  4546 333 0.1000 0.0003 0.0479 2.0865 0.0063 1.0000 3.0054 0.0090 1.4404 18.4687  4547 PNMD 325 0.0711 0.0002 0.0376 1.8916 0.0058 1.0000 3.1696 0.0097 1.6756 17.7000  4548 349 0.0986 0.0003 0.0514 1.9179 0.0055 1.0000 3.1811 0.0091 1.6586 18.0740  4550 359 0.0896 0.0002 0.0434 2.0657 0.0057 1.0000 3.2526 0.0091 1.5746 18.0449  4551 317 0.0796 0.0003 0.0438 1.8178 0.0057 1.0000 3.1310 0.0099 1.7224 16.0040 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT PNMD=PREGNANT, NO MATING DATE 
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Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP I 0 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 1526 319 1.3591 0.0043 0.7208 0.0116 0.0000 0.0062 0.7950 0.0025 0.4216  1528 350 1.7753 0.0051 0.8015 0.0117 0.0000 0.0053 0.7311 0.0021 0.3301  1529 PNMD 358 1.6839 0.0047 0.7613 0.0114 0.0000 0.0052 0.7129 0.0020 0.3223  1530 341 1.6074 0.0047 0.7848 0.0156 0.0000 0.0076 0.8858 0.0026 0.4325  1531 347 1.4982 0.0043 0.7037 0.0150 0.0000 0.0070 0.7081 0.0020 0.3326  1533 339 1.9369 0.0057 0.9346 0.0144 0.0000 0.0069 0.7417 0.0022 0.3579  1534 317 1.5106 0.0048 0.8221 0.0165 0.0001 0.0090 0.6681 0.0021 0.3636  1536 322 1.2301 0.0038 0.6773 0.0166 0.0001 0.0091 0.6222 0.0019 0.3426  1537 302 1.5359 0.0051 0.7956 0.0140 0.0000 0.0073 0.6294 0.0021 0.3260  1538 320 1.3536 0.0042 0.7286 0.0153 0.0000 0.0082 0.5974 0.0019 0.3216  1539 330 1.7169 0.0052 0.8395 0.0166 0.0001 0.0081 0.8462 0.0026 0.4137  1540 320 1.5956 0.0050 0.8288 0.0136 0.0000 0.0071 0.6587 0.0021 0.3421  1542 347 1.6463 0.0047 0.8498 0.0131 0.0000 0.0068 0.9854 0.0028 0.5087  1543 320 1.4105 0.0044 0.7371 0.0135 0.0000 0.0071 0.6787 0.0021 0.3547  1544 360 1.5207 0.0042 0.7710 0.0162 0.0000 0.0082 0.7281 0.0020 0.3692  1545 381 1.5751 0.0041 0.7467 0.0157 0.0000 0.0074 0.7540 0.0020 0.3574  1546 299 1.5459 0.0052 0.7907 0.0106 0.0000 0.0054 0.7774 0.0026 0.3976  1547 309 1.3357 0.0043 0.7379 0.0131 0.0000 0.0072 0.5591 0.0018 0.3089  1549 332 1.6857 0.0051 0.8415 0.0086 0.0000 0.0043 0.5718 0.0017 0.2855  1551 286 1.3778 0.0048 0.7449 0.0181 0.0001 0.0098 0.5961 0.0021 0.3223 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT PNMD=PREGNANT, NO MATING DATE 
23-JUN-2009 20:42 

Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP II 2000 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 2526 320 1.5004 0.0047 0.7636 0.0176 0.0001 0.0090 0.5283 0.0017 0.2689  2528 315 1.5037 0.0048 0.8039 0.0131 0.0000 0.0070 0.6025 0.0019 0.3221  2529 348 1.6356 0.0047 0.7885 0.0118 0.0000 0.0057 0.7276 0.0021 0.3508  2530 371 1.6208 0.0044 0.7619 0.0137 0.0000 0.0064 0.5416 0.0015 0.2546  2531 310 1.6434 0.0053 0.8668 0.0155 0.0000 0.0082 0.7439 0.0024 0.3924  2533 334 1.5393 0.0046 0.7692 0.0161 0.0000 0.0080 0.5957 0.0018 0.2977  2534 295 1.6789 0.0057 0.8405 0.0159 0.0001 0.0080 0.5153 0.0017 0.2580  2535 349 1.7536 0.0050 0.9542 0.0108 0.0000 0.0059 0.6562 0.0019 0.3571  2536 338 1.4730 0.0044 0.7167 0.0123 0.0000 0.0060 0.6362 0.0019 0.3096  2537 308 1.5744 0.0051 0.8369 0.0148 0.0000 0.0079 0.4934 0.0016 0.2623  2539 344 1.6275 0.0047 0.8657 0.0184 0.0001 0.0098 0.6572 0.0019 0.3496  2540 316 1.4192 0.0045 0.7097 0.0142 0.0000 0.0071 0.5383 0.0017 0.2692  2541 392 1.9041 0.0049 0.9902 0.0223 0.0001 0.0116 0.9280 0.0024 0.4826  2543 343 1.6752 0.0049 0.8381 0.0118 0.0000 0.0059 0.7869 0.0023 0.3937  2544 337 1.6066 0.0048 0.7791 0.0185 0.0001 0.0090 0.6668 0.0020 0.3234  2545 319 1.5440 0.0048 0.7681 0.0141 0.0000 0.0070 0.6414 0.0020 0.3191  2546 292 1.5170 0.0052 0.7662 0.0179 0.0001 0.0090 0.5453 0.0019 0.2754  2547 286 1.2356 0.0043 0.6529 0.0103 0.0000 0.0054 0.4857 0.0017 0.2567  2548 342 1.5003 0.0044 0.7643 0.0132 0.0000 0.0067 0.5746 0.0017 0.2927  2550 325 1.5010 0.0046 0.7785 0.0151 0.0000 0.0078 0.6181 0.0019 0.3206  2551 333 1.5263 0.0046 0.7502 0.0172 0.0001 0.0085 0.6552 0.0020 0.3221 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
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Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP III 10000 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 3527 355 1.7438 0.0049 0.8263 0.0058 0.0000 0.0027 0.6935 0.0020 0.3286  3528 307 1.4060 0.0046 0.7440 0.0091 0.0000 0.0048 0.5148 0.0017 0.2724  3529 305 1.5504 0.0051 0.8024 0.0160 0.0001 0.0083 0.7111 0.0023 0.3680  3530 325 1.5954 0.0049 0.8302 0.0159 0.0000 0.0083 0.6143 0.0019 0.3196  3531 318 1.5147 0.0048 0.8093 0.0030 0.0000 0.0016 0.6419 0.0020 0.3430  3532 362 1.8452 0.0051 0.9464 0.0081 0.0000 0.0042 0.8715 0.0024 0.4470  3533 357 1.6252 0.0045 0.8182 0.0123 0.0000 0.0062 0.6495 0.0018 0.3270  3534 313 1.4891 0.0048 0.7725 0.0187 0.0001 0.0097 0.6075 0.0019 0.3152  3535 345 1.3353 0.0039 0.6831 0.0130 0.0000 0.0067 0.5742 0.0017 0.2938  3537 312 1.4879 0.0048 0.7427 0.0128 0.0000 0.0064 0.6773 0.0022 0.3381  3540 321 1.5540 0.0048 0.7711 0.0111 0.0000 0.0055 0.6196 0.0019 0.3074  3541 326 1.5029 0.0046 0.8239 0.0141 0.0000 0.0077 0.5714 0.0018 0.3132  3542 355 1.6227 0.0046 0.7678 0.0140 0.0000 0.0066 0.8477 0.0024 0.4011  3543 310 1.4825 0.0048 0.7674 0.0151 0.0000 0.0078 0.5341 0.0017 0.2765  3544 304 1.4301 0.0047 0.7168 0.0137 0.0000 0.0069 0.5683 0.0019 0.2848  3545 326 1.6313 0.0050 0.8644 0.0156 0.0000 0.0083 0.6818 0.0021 0.3613  3546 345 1.5147 0.0044 0.7697 0.0155 0.0000 0.0079 0.6342 0.0018 0.3223  3547 324 1.5820 0.0049 0.8185 0.0159 0.0000 0.0082 0.5541 0.0017 0.2867  3549 329 1.5177 0.0046 0.7358 0.0152 0.0000 0.0074 0.5214 0.0016 0.2528  3551 337 1.5234 0.0045 0.7607 0.0160 0.0000 0.0080 0.6370 0.0019 0.3181 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT 
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Huntingdon Life Sciences 00-4206  APPENDIX Y  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  ORGAN AND FINAL BODY WEIGHTS FEMALES GROUP IV 20000 MG/M3 ------------------------------------------------------------------------------------------------------------------ LUNGS PITUITARY SPLEEN  Animal FINAL BODY ------------------------- ------------------------- -------------------------  Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain  g g g g ------------------------------------------------------------------------------------------------------------------ 4526 292 1.4390 0.0049 0.6994 0.0119 0.0000 0.0058 0.5538 0.0019 0.2692  4527 348 1.7250 0.0050 0.7995 0.0148 0.0000 0.0069 0.7691 0.0022 0.3565  4528 336 1.3700 0.0041 0.6912 0.0071 0.0000 0.0036 0.5424 0.0016 0.2736  4529 324 1.5672 0.0048 0.8016 0.0116 0.0000 0.0059 0.5796 0.0018 0.2965  4531 318 1.3459 0.0042 0.7134 0.0144 0.0000 0.0076 0.6365 0.0020 0.3374  4532 321 1.5009 0.0047 0.7370 0.0102 0.0000 0.0050 0.5041 0.0016 0.2475  4533 359 1.6258 0.0045 0.8388 0.0143 0.0000 0.0074 0.5640 0.0016 0.2910  4534 330 1.5198 0.0046 0.7803 0.0121 0.0000 0.0062 0.5780 0.0018 0.2968  4535 277 1.2940 0.0047 0.6693 0.0146 0.0001 0.0076 0.5542 0.0020 0.2867  4536 321 1.5455 0.0048 0.7362 0.0136 0.0000 0.0065 0.5123 0.0016 0.2440  4537 361 1.5636 0.0043 0.8043 0.0115 0.0000 0.0059 0.5744 0.0016 0.2955  4538 291 1.4900 0.0051 0.7652 0.0141 0.0000 0.0072 0.5983 0.0021 0.3073  4540 304 1.5125 0.0050 0.7948 0.0137 0.0000 0.0072 0.6391 0.0021 0.3358  4542 314 1.4750 0.0047 0.7952 0.0174 0.0001 0.0094 0.6495 0.0021 0.3502  4543 350 1.6689 0.0048 0.8824 0.0156 0.0000 0.0082 0.6530 0.0019 0.3453  4544 303 1.5542 0.0051 0.7909 0.0155 0.0001 0.0079 0.6200 0.0020 0.3155  4545 322 1.5360 0.0048 0.7574 0.0131 0.0000 0.0065 0.4642 0.0014 0.2289  4546 333 1.7892 0.0054 0.8575 0.0169 0.0001 0.0081 0.7455 0.0022 0.3573  4547 PNMD 325 1.4298 0.0044 0.7559 0.0123 0.0000 0.0065 0.7135 0.0022 0.3772  4548 349 1.5524 0.0045 0.8094 0.0168 0.0000 0.0088 0.6485 0.0019 0.3381  4550 359 1.4954 0.0042 0.7239 0.0122 0.0000 0.0059 0.5713 0.0016 0.2766  4551 317 1.4236 0.0045 0.7831 0.0132 0.0000 0.0073 0.5951 0.0019 0.3274 ------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT PNMD=PREGNANT, NO MATING DATE 
Huntingdon Life Sciences 00-4206 Page 495 

211-TBA-1G Final Report 

Individual Pup Organ Weights 

Preface Appendix Z 

Table of Contents 

Organ Weights and Organ/Body Weight Ratios ..............................................................496 

Organ Weights and Organ/Brain Weight Ratios .............................................................512 
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Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP I 0 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1526 4 M 81.2 1.4662 1.8057 0.4375 0.5388 0.3820 0.4704  1528 7 M 75.3 1.4576 1.9357 0.2902 0.3854 0.3140 0.4170  1529 9 M 85.0 1.5902 1.8708 0.3638 0.4280 0.3794 0.4464  1530 6 M 81.4 1.4794 1.8174 0.3220 0.3956 0.2276 0.2796  1531 6 M 80.5 1.6676 2.0716 0.3587 0.4456 0.3317 0.4120  1533 8 M 76.5 1.5730 2.0562 0.3300 0.4314 0.4077 0.5329  1534 4 M 85.6 1.5476 1.8079 0.3381 0.3950 0.3183 0.3718  1536 4 M 58.9 1.3937 2.3662 0.2669 0.4531 0.2490 0.4228  1537 6 M 75.0 1.6971 2.2628 0.3518 0.4691 0.3689 0.4919  1538 3 M 85.1 1.6348 1.9210 0.3408 0.4005 0.4317 0.5073  1539 6 M 71.0 1.5964 2.2485 0.3018 0.4251 0.2845 0.4007  1540 1 M 94.1 1.5845 1.6838 0.4186 0.4448 0.4031 0.4284  1542 3 M 83.9 1.5730 1.8749 0.3482 0.4150 0.4631 0.5520  1543 4 M 100.3 1.5916 1.5868 0.3998 0.3986 0.4749 0.4735  1544 3 M 95.8 1.5413 1.6089 0.3117 0.3254 0.4063 0.4241  1545 8 M 78.6 1.6895 2.1495 0.3027 0.3851 0.3718 0.4730  1546 4 M 79.4 1.5294 1.9262 0.3805 0.4792 0.3716 0.4680 
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Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP I 0 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1547 5 M 75.8 1.4437 1.9046 0.2423 0.3197 0.3477 0.4587  1549 1 M 63.9 1.3112 2.0520 0.2697 0.4221 0.2415 0.3779  1551 4 M 76.5 1.6021 2.0942 0.3852 0.5035 0.3655 0.4778 
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Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP II 2000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2526 5 M 80.2 1.6586 2.0681 0.2658 0.3314 0.3872 0.4828  2528 2 M 82.3 1.5478 1.8807 0.2972 0.3611 0.4224 0.5132  2529 3 M 72.4 1.5318 2.1157 0.2763 0.3816 0.3112 0.4298  2530 2 M 102.3 1.5732 1.5378 0.3536 0.3457 0.4290 0.4194  2531 6 M 78.7 1.5021 1.9086 0.3855 0.4898 0.4383 0.5569  2533 1 M 79.5 1.5442 1.9424 0.3489 0.4389 0.3377 0.4248  2534 1 M 76.2 1.5038 1.9735 0.2791 0.3663 0.3251 0.4266  2535 9 M 71.2 1.5193 2.1338 0.3829 0.5378 0.3195 0.4487  2536 4 M 92.3 1.5042 1.6297 0.3411 0.3696 0.3112 0.3372  2537 2 M 71.3 1.5105 2.1185 0.3422 0.4799 0.3245 0.4551  2539 7 M 87.8 1.6699 1.9019 0.3168 0.3608 0.2477 0.2821  2540 2 M 82.9 1.6289 1.9649 0.3956 0.4772 0.3991 0.4814  2541 3 M 84.8 1.6642 1.9625 0.3794 0.4474 0.2777 0.3275  2543 3 M 69.5 1.5745 2.2655 0.2969 0.4272 0.2637 0.3794  2544 2 M 83.1 1.4789 1.7797 0.2593 0.3120 0.3362 0.4046  2545 4 M 77.2 1.5038 1.9479 0.3537 0.4582 0.3097 0.4012  2546 5 M 85.5 1.6632 1.9453 0.3795 0.4439 0.3267 0.3821 
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Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP II 2000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2547 1 M 76.8 1.6349 2.1288 0.3199 0.4165 0.2786 0.3628  2548 4 M 77.8 1.5963 2.0518 0.4234 0.5442 0.3340 0.4293  2550 3 M 92.4 1.5981 1.7295 0.3502 0.3790 0.4045 0.4378  2551 1 M 96.3 1.6928 1.7578 0.2827 0.2936 0.2646 0.2748 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP III 10000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3527 7 M 85.9 1.5073 1.7547 0.3728 0.4340 0.3472 0.4042  3528 2 M 68.1 1.5068 2.2126 0.2470 0.3627 0.3633 0.5335  3529 7 M 70.5 1.5598 2.2125 0.3992 0.5662 0.3299 0.4679  3530 4 M 69.7 1.5995 2.2948 0.2594 0.3722 0.4297 0.6165  3531 8 M 82.2 1.5579 1.8953 0.3649 0.4439 0.3537 0.4303  3532 1 M 80.2 1.5923 1.9854 0.3791 0.4727 0.4028 0.5022  3533 7 M 83.1 1.6573 1.9943 0.3285 0.3953 0.4351 0.5236  3534 7 M 77.5 1.4399 1.8579 0.2819 0.3637 0.4220 0.5445  3535 4 M 75.8 1.6212 2.1388 0.2928 0.3863 0.3213 0.4239  3537 8 M 64.2 1.3576 2.1146 0.3257 0.5073 0.3139 0.4889  3540 2 M 70.9 1.5702 2.2147 0.2857 0.4030 0.3019 0.4258  3541 2 M 81.9 1.5827 1.9325 0.3044 0.3717 0.3573 0.4363  3542 5 M 86.8 1.6429 1.8927 0.4063 0.4681 0.3056 0.3521  3543 3 M 78.9 1.5605 1.9778 0.3281 0.4158 0.3397 0.4305  3544 3 M 78.7 1.6998 2.1598 0.3396 0.4315 0.4152 0.5276  3545 1 M 70.8 1.5110 2.1342 0.2724 0.3847 0.3294 0.4653  3546 1 M 88.5 1.6972 1.9177 0.3671 0.4148 0.3744 0.4231 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP III 10000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3547 8 M 73.2 1.5184 2.0743 0.3080 0.4208 0.3050 0.4167  3549 3 M 84.9 1.5786 1.8594 0.3492 0.4113 0.4245 0.5000  3551 4 M 102.6 1.6755 1.6330 0.4548 0.4433 0.5643 0.5500 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4526 4 M 69.5 1.5032 2.1629 0.2873 0.4134 0.3032 0.4363  4527 3 M 74.2 1.4992 2.0205 0.2866 0.3863 0.3303 0.4451  4528 4 M 75.2 1.5573 2.0709 0.3389 0.4507 0.3234 0.4301  4529 7 M 62.8 1.4582 2.3220 0.2406 0.3831 0.2605 0.4148  4531 2 M 65.8 1.5362 2.3347 0.2384 0.3623 0.2896 0.4401  4532 2 M 73.1 1.5373 2.1030 0.2342 0.3204 0.3216 0.4399  4533 5 M 84.8 1.5387 1.8145 0.2972 0.3505 0.4583 0.5404  4534 6 M 81.7 1.4248 1.7439 0.2755 0.3372 0.3027 0.3705  4535 1 M 81.4 1.5965 1.9613 0.3041 0.3736 0.3817 0.4689  4536 2 M 77.1 1.6120 2.0908 0.2132 0.2765 0.3172 0.4114  4537 3 M 74.3 1.4964 2.0140 0.2941 0.3958 0.2777 0.3738  4538 3 M 72.9 1.5527 2.1299 0.3418 0.4689 0.3554 0.4875  4540 3 M 66.1 1.4597 2.2083 0.2910 0.4402 0.3062 0.4632  4542 1 M 74.9 1.5602 2.0830 0.3493 0.4664 0.3338 0.4457  4543 4 M 77.3 1.4852 1.9213 0.2810 0.3635 0.2539 0.3285  4544 4 M 74.9 1.5156 2.0235 0.2466 0.3292 0.3058 0.4083  4545 10 M 81.6 1.5904 1.9490 0.3206 0.3929 0.3853 0.4722 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4546 7 M 77.6 1.5982 2.0595 0.3022 0.3894 0.3446 0.4441  4547 1 M 74.7 1.4433 1.9321 0.2829 0.3787 0.2999 0.4015  4548 5 M 84.3 1.6055 1.9045 0.3843 0.4559 0.3712 0.4403  4550 4 M 85.3 1.5156 1.7768 0.3387 0.3971 0.3490 0.4091  4551 1 M 69.7 1.5156 2.1745 0.3052 0.4379 0.3427 0.4917 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP I 0 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1526 10 F 73.9 1.5050 2.0365 0.3057 0.4137 0.3198 0.4327  1528 10 F 73.8 1.4975 2.0291 0.2533 0.3432 0.3190 0.4322  1529 11 F 78.2 1.5844 2.0261 0.2751 0.3518 0.3385 0.4329  1530 9 F 67.6 1.4427 2.1342 0.2728 0.4036 0.3266 0.4831  1531 8 F 67.5 1.5932 2.3603 0.2536 0.3757 0.3044 0.4510  1533 10 F 69.7 1.5287 2.1933 0.3086 0.4428 0.3539 0.5077  1534 10 F 72.6 1.4871 2.0483 0.2604 0.3587 0.3590 0.4945  1536 12 F 52.3 1.3412 2.5644 0.2239 0.4281 0.1947 0.3723  1537 13 F 69.1 1.5780 2.2836 0.3141 0.4546 0.3108 0.4498  1538 10 F 74.3 1.6453 2.2144 0.3453 0.4647 0.3881 0.5223  1539 9 F 78.8 1.6369 2.0773 0.2956 0.3751 0.3566 0.4525  1540 14 F 81.9 1.4941 1.8243 0.3834 0.4681 0.3602 0.4398  1542 13 F 70.6 1.5236 2.1581 0.3161 0.4477 0.3946 0.5589  1543 15 F 77.9 1.4437 1.8533 0.2840 0.3646 0.3296 0.4231  1544 10 F 82.4 1.5117 1.8346 0.2519 0.3057 0.4839 0.5873  1545 10 F 83.4 1.6269 1.9507 0.2948 0.3535 0.4087 0.4900  1546 11 F 77.3 1.4154 1.8310 0.3319 0.4294 0.3615 0.4677 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP I 0 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1547 13 F 73.7 1.4416 1.9560 0.3481 0.4723 0.3890 0.5278  1549 13 F 72.0 1.4848 2.0622 0.3687 0.5121 0.2723 0.3782  1551 6 F 80.0 1.5888 1.9860 0.3594 0.4492 0.3970 0.4963 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP II 2000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2526 13 F 77.9 1.5212 1.9528 0.2802 0.3597 0.4103 0.5267  2528 11 F 69.9 1.4666 2.0981 0.3052 0.4366 0.3699 0.5292  2529 12 F 77.1 1.4865 1.9280 0.2887 0.3744 0.3932 0.5100  2530 14 F 79.6 1.5197 1.9092 0.2592 0.3256 0.3699 0.4647  2531 12 F 70.5 1.4574 2.0672 0.3061 0.4342 0.3843 0.5451  2533 15 F 74.8 1.4805 1.9793 0.2798 0.3741 0.3771 0.5041  2534 6 F 74.9 1.4331 1.9134 0.2968 0.3963 0.3674 0.4905  2535 13 F 54.7 1.3557 2.4784 0.2304 0.4212 0.3047 0.5570  2536 16 F 75.5 1.4340 1.8993 0.2546 0.3372 0.2881 0.3816  2537 7 F 70.7 1.5016 2.1239 0.2816 0.3983 0.4132 0.5844  2539 9 F 73.8 1.3791 1.8687 0.2919 0.3955 0.3205 0.4343  2540 9 F 75.2 1.6194 2.1535 0.3625 0.4820 0.3642 0.4843  2541 8 F 81.1 1.6324 2.0128 0.3703 0.4566 0.4311 0.5316  2543 10 F 69.1 1.5538 2.2486 0.3396 0.4915 0.3618 0.5236  2544 3 F 76.0 1.4702 1.9345 0.2938 0.3866 0.3654 0.4808  2545 8 F 68.8 1.4535 2.1126 0.3321 0.4827 0.3538 0.5142  2546 13 F 67.7 1.4992 2.2145 0.2516 0.3716 0.2520 0.3722 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP II 2000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2547 9 F 78.0 1.5616 2.0021 0.3267 0.4188 0.3181 0.4078  2548 8 F 78.5 1.6389 2.0878 0.3490 0.4446 0.3600 0.4586  2550 8 F 82.9 1.5717 1.8959 0.3598 0.4340 0.3987 0.4809  2551 12 F 82.6 1.5823 1.9156 0.3266 0.3954 0.3946 0.4777 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP III 10000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3527 17 F 78.4 1.4353 1.8307 0.3123 0.3983 0.3991 0.5091  3528 12 F 61.8 1.4357 2.3231 0.2300 0.3722 0.3103 0.5021  3529 10 F 80.8 1.6082 1.9903 0.4290 0.5309 0.3172 0.3926  3530 9 F 71.9 1.5833 2.2021 0.2848 0.3961 0.4238 0.5894  3531 11 F 74.3 1.5203 2.0462 0.2528 0.3402 0.4032 0.5427  3532 11 F 72.4 1.4882 2.0555 0.3472 0.4796 0.3093 0.4272  3533 11 F 86.3 1.5715 1.8210 0.3271 0.3790 0.5430 0.6292  3534 8 F 76.5 1.4586 1.9067 0.3340 0.4366 0.3844 0.5025  3535 7 F 69.3 1.4507 2.0934 0.3344 0.4825 0.3139 0.4530  3537 12 F 63.3 1.4542 2.2973 0.2851 0.4504 0.3712 0.5864  3540 12 F 59.5 1.5167 2.5491 0.2512 0.4222 0.2793 0.4694  3541 8 F 74.8 1.4917 1.9943 0.3230 0.4318 0.4042 0.5404  3542 11 F 77.0 1.5517 2.0152 0.3293 0.4277 0.3808 0.4945  3543 4 F 69.4 1.5094 2.1749 0.2195 0.3163 0.3196 0.4605  3544 10 F 72.1 1.6425 2.2781 0.2762 0.3831 0.3566 0.4946  3545 14 F 64.9 1.5169 2.3373 0.2749 0.4236 0.3368 0.5190  3546 15 F 77.6 1.5901 2.0491 0.3030 0.3905 0.3732 0.4809 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP III 10000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3547 14 F 66.6 1.4402 2.1625 0.2427 0.3644 0.3143 0.4719  3549 11 F 80.2 1.5666 1.9534 0.3158 0.3938 0.4554 0.5678 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4526 15 F 58.5 1.4212 2.4294 0.2816 0.4814 0.3387 0.5790  4527 11 F 67.5 1.4317 2.1210 0.2417 0.3581 0.3008 0.4456  4528 12 F 63.8 1.5089 2.3650 0.2719 0.4262 0.3514 0.5508  4529 12 F 67.2 1.3413 1.9960 0.2294 0.3414 0.2726 0.4057  4531 8 F 66.5 1.4198 2.1350 0.2951 0.4438 0.3369 0.5066  4532 14 F 52.3 1.3287 2.5405 0.1691 0.3233 0.2382 0.4554  4533 13 F 78.4 1.4541 1.8547 0.2782 0.3548 0.4788 0.6107  4534 14 F 63.6 1.6255 2.5558 0.3514 0.5525 0.4048 0.6365  4535 6 F 59.6 1.3900 2.3322 0.2059 0.3455 0.2388 0.4007  4536 14 F 68.3 1.5484 2.2671 0.2536 0.3713 0.3801 0.5565  4537 11 F 85.8 1.5585 1.8164 0.2984 0.3478 0.4207 0.4903  4538 15 F 73.6 1.5644 2.1255 0.2617 0.3556 0.4235 0.5754  4540 11 F 65.6 1.4976 2.2829 0.2629 0.4008 0.3220 0.4909  4542 10 F 68.6 1.4896 2.1714 0.2468 0.3598 0.3337 0.4864  4543 7 F 76.4 1.4954 1.9573 0.3038 0.3976 0.4368 0.5717  4544 15 F 70.7 1.4402 2.0371 0.2363 0.3342 0.3187 0.4508  4545 13 F 75.8 1.5201 2.0054 0.2483 0.3276 0.3577 0.4719 
23-JUN-2009 21:29 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ Final BRAIN SPLEEN THYMUS  Animal Pup Body -------------- -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4546 16 F 74.6 1.5655 2.0985 0.3002 0.4024 0.3597 0.4822  4547 7 F 63.2 1.3757 2.1767 0.2622 0.4149 0.3232 0.5114  4548 11 F 75.4 1.5421 2.0452 0.3167 0.4200 0.4014 0.5324  4550 15 F 66.3 1.4782 2.2296 0.2289 0.3452 0.3199 0.4825  4551 12 F 64.8 1.4172 2.1870 0.2550 0.3935 0.3410 0.5262 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP I 0 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1526 4 M 1.4662 0.4375 29.839 0.3820 26.054  1528 7 M 1.4576 0.2902 19.909 0.3140 21.542  1529 9 M 1.5902 0.3638 22.878 0.3794 23.859  1530 6 M 1.4794 0.3220 21.766 0.2276 15.385  1531 6 M 1.6676 0.3587 21.510 0.3317 19.891  1533 8 M 1.5730 0.3300 20.979 0.4077 25.919  1534 4 M 1.5476 0.3381 21.847 0.3183 20.567  1536 4 M 1.3937 0.2669 19.150 0.2490 17.866  1537 6 M 1.6971 0.3518 20.729 0.3689 21.737  1538 3 M 1.6348 0.3408 20.847 0.4317 26.407  1539 6 M 1.5964 0.3018 18.905 0.2845 17.821  1540 1 M 1.5845 0.4186 26.418 0.4031 25.440  1542 3 M 1.5730 0.3482 22.136 0.4631 29.441  1543 4 M 1.5916 0.3998 25.119 0.4749 29.838  1544 3 M 1.5413 0.3117 20.223 0.4063 26.361  1545 8 M 1.6895 0.3027 17.917 0.3718 22.007  1546 4 M 1.5294 0.3805 24.879 0.3716 24.297 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP I 0 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1547 5 M 1.4437 0.2423 16.783 0.3477 24.084  1549 1 M 1.3112 0.2697 20.569 0.2415 18.418  1551 4 M 1.6021 0.3852 24.043 0.3655 22.814 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP II 2000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2526 5 M 1.6586 0.2658 16.026 0.3872 23.345  2528 2 M 1.5478 0.2972 19.201 0.4224 27.290  2529 3 M 1.5318 0.2763 18.038 0.3112 20.316  2530 2 M 1.5732 0.3536 22.476 0.4290 27.269  2531 6 M 1.5021 0.3855 25.664 0.4383 29.179  2533 1 M 1.5442 0.3489 22.594 0.3377 21.869  2534 1 M 1.5038 0.2791 18.560 0.3251 21.619  2535 9 M 1.5193 0.3829 25.202 0.3195 21.029  2536 4 M 1.5042 0.3411 22.677 0.3112 20.689  2537 2 M 1.5105 0.3422 22.655 0.3245 21.483  2539 7 M 1.6699 0.3168 18.971 0.2477 14.833  2540 2 M 1.6289 0.3956 24.286 0.3991 24.501  2541 3 M 1.6642 0.3794 22.798 0.2777 16.687  2543 3 M 1.5745 0.2969 18.857 0.2637 16.748  2544 2 M 1.4789 0.2593 17.533 0.3362 22.733  2545 4 M 1.5038 0.3537 23.520 0.3097 20.594  2546 5 M 1.6632 0.3795 22.817 0.3267 19.643 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP II 2000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2547 1 M 1.6349 0.3199 19.567 0.2786 17.041  2548 4 M 1.5963 0.4234 26.524 0.3340 20.923  2550 3 M 1.5981 0.3502 21.914 0.4045 25.311  2551 1 M 1.6928 0.2827 16.700 0.2646 15.631 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP III 10000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3527 7 M 1.5073 0.3728 24.733 0.3472 23.035  3528 2 M 1.5068 0.2470 16.392 0.3633 24.111  3529 7 M 1.5598 0.3992 25.593 0.3299 21.150  3530 4 M 1.5995 0.2594 16.218 0.4297 26.865  3531 8 M 1.5579 0.3649 23.423 0.3537 22.704  3532 1 M 1.5923 0.3791 23.808 0.4028 25.297  3533 7 M 1.6573 0.3285 19.821 0.4351 26.254  3534 7 M 1.4399 0.2819 19.578 0.4220 29.308  3535 4 M 1.6212 0.2928 18.061 0.3213 19.819  3537 8 M 1.3576 0.3257 23.991 0.3139 23.122  3540 2 M 1.5702 0.2857 18.195 0.3019 19.227  3541 2 M 1.5827 0.3044 19.233 0.3573 22.575  3542 5 M 1.6429 0.4063 24.731 0.3056 18.601  3543 3 M 1.5605 0.3281 21.025 0.3397 21.769  3544 3 M 1.6998 0.3396 19.979 0.4152 24.426  3545 1 M 1.5110 0.2724 18.028 0.3294 21.800  3546 1 M 1.6972 0.3671 21.630 0.3744 22.060 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP III 10000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3547 8 M 1.5184 0.3080 20.285 0.3050 20.087  3549 3 M 1.5786 0.3492 22.121 0.4245 26.891  3551 4 M 1.6755 0.4548 27.144 0.5643 33.679 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4526 4 M 1.5032 0.2873 19.113 0.3032 20.170  4527 3 M 1.4992 0.2866 19.117 0.3303 22.032  4528 4 M 1.5573 0.3389 21.762 0.3234 20.767  4529 7 M 1.4582 0.2406 16.500 0.2605 17.864  4531 2 M 1.5362 0.2384 15.519 0.2896 18.852  4532 2 M 1.5373 0.2342 15.235 0.3216 20.920  4533 5 M 1.5387 0.2972 19.315 0.4583 29.785  4534 6 M 1.4248 0.2755 19.336 0.3027 21.245  4535 1 M 1.5965 0.3041 19.048 0.3817 23.909  4536 2 M 1.6120 0.2132 13.226 0.3172 19.677  4537 3 M 1.4964 0.2941 19.654 0.2777 18.558  4538 3 M 1.5527 0.3418 22.013 0.3554 22.889  4540 3 M 1.4597 0.2910 19.936 0.3062 20.977  4542 1 M 1.5602 0.3493 22.388 0.3338 21.395  4543 4 M 1.4852 0.2810 18.920 0.2539 17.095  4544 4 M 1.5156 0.2466 16.271 0.3058 20.177  4545 10 M 1.5904 0.3206 20.158 0.3853 24.227 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 MALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4546 7 M 1.5982 0.3022 18.909 0.3446 21.562  4547 1 M 1.4433 0.2829 19.601 0.2999 20.779  4548 5 M 1.6055 0.3843 23.936 0.3712 23.121  4550 4 M 1.5156 0.3387 22.348 0.3490 23.027  4551 1 M 1.5156 0.3052 20.137 0.3427 22.612 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP I 0 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1526 10 F 1.5050 0.3057 20.312 0.3198 21.249  1528 10 F 1.4975 0.2533 16.915 0.3190 21.302  1529 11 F 1.5844 0.2751 17.363 0.3385 21.365  1530 9 F 1.4427 0.2728 18.909 0.3266 22.638  1531 8 F 1.5932 0.2536 15.918 0.3044 19.106  1533 10 F 1.5287 0.3086 20.187 0.3539 23.150  1534 10 F 1.4871 0.2604 17.511 0.3590 24.141  1536 12 F 1.3412 0.2239 16.694 0.1947 14.517  1537 13 F 1.5780 0.3141 19.905 0.3108 19.696  1538 10 F 1.6453 0.3453 20.987 0.3881 23.588  1539 9 F 1.6369 0.2956 18.059 0.3566 21.785  1540 14 F 1.4941 0.3834 25.661 0.3602 24.108  1542 13 F 1.5236 0.3161 20.747 0.3946 25.899  1543 15 F 1.4437 0.2840 19.672 0.3296 22.830  1544 10 F 1.5117 0.2519 16.663 0.4839 32.010  1545 10 F 1.6269 0.2948 18.120 0.4087 25.121  1546 11 F 1.4154 0.3319 23.449 0.3615 25.540 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP I 0 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 1547 13 F 1.4416 0.3481 24.147 0.3890 26.984  1549 13 F 1.4848 0.3687 24.832 0.2723 18.339  1551 6 F 1.5888 0.3594 22.621 0.3970 24.987 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP II 2000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2526 13 F 1.5212 0.2802 18.420 0.4103 26.972  2528 11 F 1.4666 0.3052 20.810 0.3699 25.222  2529 12 F 1.4865 0.2887 19.421 0.3932 26.451  2530 14 F 1.5197 0.2592 17.056 0.3699 24.340  2531 12 F 1.4574 0.3061 21.003 0.3843 26.369  2533 15 F 1.4805 0.2798 18.899 0.3771 25.471  2534 6 F 1.4331 0.2968 20.710 0.3674 25.637  2535 13 F 1.3557 0.2304 16.995 0.3047 22.475  2536 16 F 1.4340 0.2546 17.755 0.2881 20.091  2537 7 F 1.5016 0.2816 18.753 0.4132 27.517  2539 9 F 1.3791 0.2919 21.166 0.3205 23.240  2540 9 F 1.6194 0.3625 22.385 0.3642 22.490  2541 8 F 1.6324 0.3703 22.684 0.4311 26.409  2543 10 F 1.5538 0.3396 21.856 0.3618 23.285  2544 3 F 1.4702 0.2938 19.984 0.3654 24.854  2545 8 F 1.4535 0.3321 22.848 0.3538 24.341  2546 13 F 1.4992 0.2516 16.782 0.2520 16.809 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP II 2000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 2547 9 F 1.5616 0.3267 20.921 0.3181 20.370  2548 8 F 1.6389 0.3490 21.295 0.3600 21.966  2550 8 F 1.5717 0.3598 22.892 0.3987 25.367  2551 12 F 1.5823 0.3266 20.641 0.3946 24.938 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP III 10000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3527 17 F 1.4353 0.3123 21.759 0.3991 27.806  3528 12 F 1.4357 0.2300 16.020 0.3103 21.613  3529 10 F 1.6082 0.4290 26.676 0.3172 19.724  3530 9 F 1.5833 0.2848 17.988 0.4238 26.767  3531 11 F 1.5203 0.2528 16.628 0.4032 26.521  3532 11 F 1.4882 0.3472 23.330 0.3093 20.783  3533 11 F 1.5715 0.3271 20.815 0.5430 34.553  3534 8 F 1.4586 0.3340 22.899 0.3844 26.354  3535 7 F 1.4507 0.3344 23.051 0.3139 21.638  3537 12 F 1.4542 0.2851 19.605 0.3712 25.526  3540 12 F 1.5167 0.2512 16.562 0.2793 18.415  3541 8 F 1.4917 0.3230 21.653 0.4042 27.097  3542 11 F 1.5517 0.3293 21.222 0.3808 24.541  3543 4 F 1.5094 0.2195 14.542 0.3196 21.174  3544 10 F 1.6425 0.2762 16.816 0.3566 21.711  3545 14 F 1.5169 0.2749 18.122 0.3368 22.203  3546 15 F 1.5901 0.3030 19.055 0.3732 23.470 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP III 10000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 3547 14 F 1.4402 0.2427 16.852 0.3143 21.823  3549 11 F 1.5666 0.3158 20.158 0.4554 29.069 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4526 15 F 1.4212 0.2816 19.814 0.3387 23.832  4527 11 F 1.4317 0.2417 16.882 0.3008 21.010  4528 12 F 1.5089 0.2719 18.020 0.3514 23.288  4529 12 F 1.3413 0.2294 17.103 0.2726 20.324  4531 8 F 1.4198 0.2951 20.785 0.3369 23.729  4532 14 F 1.3287 0.1691 12.727 0.2382 17.927  4533 13 F 1.4541 0.2782 19.132 0.4788 32.928  4534 14 F 1.6255 0.3514 21.618 0.4048 24.903  4535 6 F 1.3900 0.2059 14.813 0.2388 17.180  4536 14 F 1.5484 0.2536 16.378 0.3801 24.548  4537 11 F 1.5585 0.2984 19.147 0.4207 26.994  4538 15 F 1.5644 0.2617 16.728 0.4235 27.071  4540 11 F 1.4976 0.2629 17.555 0.3220 21.501  4542 10 F 1.4896 0.2468 16.568 0.3337 22.402  4543 7 F 1.4954 0.3038 20.316 0.4368 29.210  4544 15 F 1.4402 0.2363 16.407 0.3187 22.129  4545 13 F 1.5201 0.2483 16.334 0.3577 23.531 
23-JUN-2009 21:31 

Huntingdon Life Sciences 00-4206  APPENDIX Z  GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY  INHALATION REPRODUCTION TOXICITY STUDY IN RATS  INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) GROUP IV 20000 MG/M3 FEMALE PUPS ------------------------------------------------------------------------------------------------------------------ SPLEEN THYMUS  Animal Pup Brain -------------- --------------  Number No. Sex Weight Weight Ratio Weight Ratio ------------------------------------------------------------------------------------------------------------------ 4546 16 F 1.5655 0.3002 19.176 0.3597 22.977  4547 7 F 1.3757 0.2622 19.059 0.3232 23.493  4548 11 F 1.5421 0.3167 20.537 0.4014 26.029  4550 15 F 1.4782 0.2289 15.485 0.3199 21.641  4551 12 F 1.4172 0.2550 17.993 0.3410 24.062 
Huntingdon Life Sciences 00-4206 Page 528 

211-TBA-1G Final Report

Individual Animal Gross 

and Microscopic Observations 

Preface Appendix AA 

Key to Abbreviations 

Gl = Gland 

LN = Lymph Node 

Notes

1. Unless otherwise specified in a histopathology note, the organ/tissue exam ined was 

the required (routine) section. 

2. The dimensions of gross lesions are presen ted as a general range based on the actual 

dimensions noted during macroscopic examinations. The actual dim ensions of gross 

lesions can be found in the raw data. 

Corresponding exposure levels for each group were as follows: 

Group I - 0 mg/m3 

Group IV - 20000 mg/m3 










































AUDITED FINAL REPORT 

Study Phase: Sperm Analysis

Test Site Phase Reference Number 93075134 

Testing Facility Study Number 00-4206 

Sponsor Reference Number 211-TBA-1G 

Gasoline TBA Vapor Condensate: 

A One-Generation Whole-Body Inhalation Reproduction 

Toxicity Study in Rats 

TEST SITE: 

Charles River Laboratories, Pathology Associates - Maryland 

15 Worman’s Mill Court, Suite I 

Frederick, MD 21701 

TESTING FACILITY: 

Huntington Life Sciences (HLS) 

Mettlers Road 

P.O. Box 2360 

East Millstone, NJ 08875-2360 

SPONSOR: 

American Petroleum Institute (API) 

1220 L Street, Northwest 

Washington, D.C. 20005-4070 

09-APR-2009 

Page 1 of 14 

Appendix BB Page 571
Final Report 

HLS Study No.: 00-4206 

2

TABLE OF CONTENTS 

Section Page No.

I. QUALITY ASSURANCE STATEMENT .............................. 4

II. MATERIALS AND METHODS .................................... 5

A. Sample Collection................................... 5

B. Sperm Motility Evaluation........................... 5

C. Caudal Epididymis Weight and Total Sperm Count 

Determination ...................................... 6

D. Sperm Morphology Evaluation......................... 6

E. Statistical Analyses................................ 6

F. GLP Compliance Statement............................ 7

G. Sample and Data Archiving........................... 7

III. RESULTS .................................................. 7

A. Sperm Motility...................................... 7

B. Total Sperm Count................................... 7

C. Sperm Morphology.................................... 8

D. Caudal Epididymis Weight............................ 8

IV. DISCUSSION AND CONCLUSIONS ............................... 8

V. REFERENCES ............................................... 9

Page 572
Final Report 

HLS Study No.: 00-4206 

3

INDEX OF TABLES AND APPENDICES 

Table Page No.

 1. Summary of Male Reproductive Parameters .................10 

Appendix Page No.

A. Individual Caudal Epididymis Weight, 

 Sperm Motility and Sperm Count Data .....................11 

B. Individual Sperm Morphology Data .........................13 

Page 573

Final Report 

HLS Study No.: 00-4206 

5

Gasoline TBA Vapor Condensate: 

A One-Generation Whole-Body Inhalation Reproduction Toxicity 

Study in Rats 

 II. MATERIALS AND METHODS 

 A. Sample Collection

 All surviving male rats were euthanized by exsanguination 

following anesthesia with inhaled carbon dioxide after the last F1

litters were weaned. For all animals, the abdominal cavity was 

opened and the reproductive organs exposed. For motility 

assessment, the left vas deferens was dissected away from the 

testis and immediately placed in a petri dish containing 10 ml of 

a solution consisting of 1% Bovine Serum Albumin dissolved in 

Phosphate Buffered Saline. The solution was prewarmed to a 

temperature of approximately 38o

C. A minimum 3-minute period was 

allowed for the sperm to swim out. 

 For total sperm count assessment, the left epididymis and 

left testis were then removed and placed on dry ice. The frozen 

epididymides and testes were then transferred to Pathology 

Associates, A Division of Charles River Laboratories, Inc. 

(formerly known as Pathology Associates International until 

January 8, 2001), Frederick, MD. and stored frozen at -70oC until 

evaluation for caudal epididymal sperm count and homogenizationresistant testicular sperm count. 

 Only the control and high-exposure animals were examined for 

percent motility, total epididymal sperm, homogenization-resistant 

testicular spermatid count, sperm morphology and caudal epididymis 

weight. 

 B. Sperm Motility Evaluation

 Following the swim out period, a sperm sample was obtained 

using a 100 μm deep cannula. The cannula was immediately loaded 

into the prewarmed stage of the Hamilton Thorne IVOS (Ver. 12.1c) 

automated sperm analyzer. The analyzer automatically selected 

five fields and each motion image was digitally saved and 

permanently stored on optical media. The images were 

subsequently analyzed and the percent motility determined for 

each animal. 

Page 575
Final Report 

HLS Study No.: 00-4206 

6

 C. Caudal Epididymis Weight and Total Sperm Count Determination

 Each frozen epididymis was removed from the freezer, thawed 

and the caudal section was trimmed and weighed. Each frozen 

testis was removed from the freezer, thawed, the tunica removed 

and the testicular parenchyma weighed. The cauda epididymis and 

testicular parenchyma were homogenized in deionized water and the 

suspensions were transferred to plastic test tubes and vortexed. A 

100 μl sample was transferred to a violet reaction vial containing 

a Hoechst dye (H33342) which uniquely stains the head of the 

sperm. A sample of the stained sperm was placed into a 20 μm deep 

Cell-Vu glass slide which was loaded into the Hamilton Thorne IVOS 

(Ver. 12.1c) analyzer. Twenty fields were automatically selected 

by the analyzer for each animal and the number of homogenizationresistant spermatid and cauda epididymal sperm determined. The 

counts reported were adjusted for caudal epididymal or testicular 

weight. 

 D. Sperm Morphology Evaluation

 Two Eosin stained slides were prepared for each animal from 

the caudal epididymis total count preparation. The slides were 

evaluated and a minimum of 200 sperm cells/animal was examined for 

morphological development. 

 E. Statistical Analyses

 The means and standard deviations for the sperm motility, 

caudal epididymal sperm count, testicular homogenization-resistant 

spermatid count and sperm morphology data were calculated and 

compared across groups using the Kruskal-Wallis nonparametric 

ANOVA test. If a significant effect occurred (p<0.05), the MannWhitney U test was used for pair-wise comparisons of each treated 

group to the control group. Animals with fewer than 25 sperm 

cells present in the motility analysis were excluded from the 

group mean calculation and subsequent statistical analysis. 

Animals with at least 25 sperm cells were included in the group 

mean calculation subsequent and statistical analysis; however, 

animals with fewer than 50 cells were identified to have a low 

count. The mean caudal epididymis weight for each group was 

calculated and compared using one-way analysis of variance (ANOVA) 

technique. If ANOVA is significant, Dunnett's test was used for 

pair-wise comparisons of each treated group to the control group 

at the 5% and 1% risk levels. Statistical analyses were 

performed using an IBMTM compatible computer with SAS computer 

programs (SAS/STAT User's Guide, 1989). 

Page 576
Final Report 

HLS Study No.: 00-4206 

7

 F. GLP Compliance Statement

 The portion of this study performed by PAI was conducted in 

compliance with US EPA 79.60, CFR Vol. 59, No. 122, 27 June 1994. 

 G. Sample and Data Archiving

 Frozen epididymis and testis samples not processed for sperm 

count determination were transferred to and stored frozen at -70o

C 

in PAI's long-term archive facility. The optical media used for 

permanent storage of the sperm motility images, raw data printouts 

from the sperm motility, epididymal and testicular sperm count and 

sperm morphology analysis, as well as any supporting documentation 

and the sperm morphology slides will be maintained at PAI until 

the study has been completed. Upon finalizing the report, all 

non-frozen study material will be returned to HLS for archiving. 

Disposition of the frozen tissue samples will be directed by 

correspondence from HLS. 

III. RESULTS 

 A. Sperm Motility

 Table 1 (Summary Data) 

 Appendix A (Individual Data) 

 No apparent treatment-related effects were observed in the 

sperm motility data. Group mean values were 89% and 90% for the 

control and high-exposure groups, respectively. There was one 

animal (animal 1042) in the control group with no motile sperm. 

 B. Total Sperm Count

 Table 1 (Summary Data) 

 Appendix A (Individual Data) 

 The number of caudal epididymal sperm and homogenizationresistant testicular spermatids were comparable between the 

control and high-exposure groups. Group mean values were 876.5 and 

793.9 million sperm/gram of epididymis and 108.3 million 

spermatids/gram of testis for both the control and high-exposure 

group. 

Page 577

Final Report 

HLS Study No.: 00-4206 

9

 V. REFERENCES 

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV 

functions for calculating probabilities associated with 

Dunnett’s test. Behav. Res. Methods and Instrumentation 13: 

363-366. 

Dunnett, C. W. 1955. A multiple comparison procedure for 

comparing several treatments with a control. Journal of the 

American Statistical Association 50:1096-1121. 

Dunnett, C. W. 1964. New tables for multiple comparisons with a 

control. Biometrics 20:482-491. 

Kruskal, W.H. and Wallis, W.A. 1952. Use of ranks in onecriterion variance analysis. Journal of the American 

Statistical Association 47: 583-621. 

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis 

(1952). Journal of the American Statistical Association 48: 

907-911. 

Kruskal, W.H. 1957. Historical notes on the Wilcoxon unpaired 

two-sample test. Journal of the American Statistical 

Association 52: 356-360. 

SAS/STAT User's Guide, 1989a. SAS Institute Inc., Version 6, 

Fourth Edition, Cary, NC:SAS Institute Inc., 1989. Vol. 1, 

p. 209-244. Vol. 2 p. 1195-1210. 

Page 579
10

HLS STUDY NO.: 00-4206 

TABLE 1 

GASOLINE TBA VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

SUMMARY OF MALE REPRODUCTIVE PARAMETERS 

Group: I IV 

Exposure Level: GTBAVCa

 (mg/m3) 0 20,000 

MOTILITY (%) 

 Mean 89 90 

 SD 6 5 

 N 25 26 

EPIDIDYMAL COUNT 

 (MILLION SPERM/GRAM) 

 Mean 876.5 793.9 

 SD 174.0 115.7 

 N 26 26 

TESTICULAR COUNT 

 (MILLION SPERM/GRAM) 

 Mean 108.3 108.3 

 SD 16.0 13.5 

 N 26 26 

SPERM MORPHOLOGYb

 (% ABNORMAL) 

 Mean 0.7 0.5 

 SD 0.6 0.5 

 N 26 26 

CAUDAL EPIDIDYMIS WEIGHTS 

 (GRAM) 

 Mean 0.28 0.26*

 SD 0.03 0.03 

 N 26 26 

a

GASOLINE TBA VAPOR CONDENSATE. b

MEAN AND STANDARD DEVIATIONS WERE CALCULATED USING THE TOTAL NUMBER OF ABNORMAL SPERM 

AS A PERCENTAGE OF THE NUMBER OF SPERM EXAMINED. 

*

SIGNIFICANTLY DIFFERENT FROM CONTROL GROUP (p<0.05). 

Page 580
11

HLS STUDY NO.: 00-4206 

APPENDIX A 

GASOLINE TBA VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT, 

SPERM MOTILITY AND SPERM COUNT DATA 

Group I: 0 mg/m3 (GASOLINE TBA VAPOR CONDENSATE) 

S P E R M C O U N T 

Animal Caudal Motility Caudal Homogenization Resistant 

 No. Epididymis Epididymal Testicular 

Weight (g) (%) (million sperm/gram) 

1026 0.25 83 1166.9 80.5 

1027 0.26 94 997.4 105.8 

1028 0.30 91 920.2 118.5 

1029 0.25 86 1422.1 123.9 

1030 0.30 90 780.7 128.6 

1031 0.30 90 853.9 100.9 

1032 0.25 94 723.6 88.8 

1033 0.32 92 1032.6 84.4 

1034 0.28 91 765.6 93.5 

1035 0.25 94 832.3 131.9 

1036 0.24 84 1019.5 120.7 

1037 0.30 89 913.2 85.7 

1038 0.27 90 782.3 104.6 

1039 0.20 94 716.3 117.6 

1040 0.33 92 817.5 97.1 

1041 0.26 88 969.2 135.5 

1042 0.28 a

 743.1 123.6 

1043 0.31 95 731.9 122.0 

1044 0.30 91 742.4 111.4 

1045 0.29 94 1031.2 117.5 

1046 0.30 95 745.9 105.2 

1047 0.30 92 979.4 98.5 

1048 0.22 89 627.4 124.4 

1049 0.28 89 855.2 110.9 

1050 0.29 68 948.3 88.0 

1051 0.24 78 670.9 97.1 

a

NO SPERM PRESENT FOR ANALYSIS. 

Page 581
12

HLS STUDY NO.: 00-4206 

APPENDIX A 

GASOLINE TBA VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT, 

SPERM MOTILITY AND SPERM COUNT DATA 

Group IV: 20,000 mg/m3 (GASOLINE TBA VAPOR CONDENSATE) 

S P E R M C O U N T 

Animal Caudal Motility Caudal Homogenization Resistant 

 No. Epididymis Epididymal Testicular 

Weight (g) (%) (million sperm/gram) 

4026 0.30 94 923.6 128.6 

4027 0.23 96 1063.8 137.9 

4028 0.23 88 600.1 118.6 

4029 0.25 83 824.0 101.6 

4030 0.28 89 832.8 82.9 

4031 0.22 92 803.2 105.1 

4032 0.29 90 757.2 114.3 

4033 0.32 92 895.3 101.9 

4034 0.26 96 744.0 129.2 

4035 0.28 84 956.0 85.3 

4037 0.20 84 569.9 109.9 

4038 0.23 95 581.9 119.5 

4039 0.26 97 852.6 109.4 

4040 0.31 94 823.0 101.4 

4041 0.23 93 681.9 127.8 

4042 0.23 93 768.3 104.6 

4043 0.25 93 828.1 112.5 

4044 0.28 77 881.3 103.9 

4045 0.28 96 911.2 98.3 

4046 0.30 95 829.5 85.0 

4047 0.28 91 858.9 105.3 

4048 0.23 89 722.9 104.6 

4049 0.26 92 744.0 106.5 

4050 0.25 86 723.6 118.0 

4051 0.21 84 687.1 101.5 

4052 0.23 86 777.4 103.2 

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APPENDIX B 

GASOLINE TBA VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL SPERM MORPHOLOGY DATA 

13

Group I: 0 mg/m3 (GASOLINE TBA VAPOR CONDENSATE) Animal ----------------H e a d---------------- --------T a i l---- No. Normal Amorphous Small Enlarged Double Coiled Bent D

1026 199 1 0 0 0 0 0 1027 197 3 0 0 0 0 0 1028 199 1 0 0 0 0 0 1029 196 4 0 0 0 0 0 1030 200 0 0 0 0 0 0 1031 199 1 0 0 0 0 0 1032 198 1 0 0 0 0 1 1033 199 1 0 0 0 0 0 1034 200 0 0 0 0 0 0 1035 198 1 1 0 0 0 0 1036 200 0 0 0 0 0 0 1037 198 1 1 0 0 0 0 1038 199 1 0 0 0 0 0 1039 200 0 0 0 0 0 0 1040 200 0 0 0 0 0 0 1041 199 1 0 0 0 0 0 1042 199 1 0 0 0 0 0 1043 200 0 0 0 0 0 0 1044 197 2 1 0 0 0 0 1045 196 4 0 0 0 0 0 1046 199 0 1 0 0 0 0 1047 200 0 0 0 0 0 0 1048 198 0 0 2 0 0 0 1049 200 0 0 0 0 0 0 1050 199 0 0 1 0 0 0 1051 197 2 1 0 0 0 0 
HLS STUDY NO.: 00-4206 

APPENDIX B 

GASOLINE TBA VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL SPERM MORPHOLOGY DATA 

14

Group IV: 20,000 mg/m3 (GASOLINE TBA VAPOR CONDENSATE) Animal ----------------H e a d---------------- --------T a i l---- No. Normal Amorphous Small Enlarged Double Coiled Bent D

4026 199 1 0 0 0 0 0 4027 197 3 0 0 0 0 0 4028 198 1 0 1 0 0 0 4029 200 0 0 0 0 0 0 4030 199 1 0 0 0 0 0 4031 198 1 1 0 0 0 0 4032 200 0 0 0 0 0 0 4033 197 3 0 0 0 0 0 4034 198 2 0 0 0 0 0 4035 199 1 0 0 0 0 0 4037 200 0 0 0 0 0 0 4038 200 0 0 0 0 0 0 4039 199 1 0 0 0 0 0 4040 200 0 0 0 0 0 0 4041 200 0 0 0 0 0 0 4042 198 2 0 0 0 0 0 4043 200 0 0 0 0 0 0 4044 199 0 1 0 0 0 0 4045 198 1 1 0 0 0 0 4046 199 1 0 0 0 0 0 4047 200 0 0 0 0 0 0 4048 199 1 0 0 0 0 0 4049 198 2 0 0 0 0 0 4050 200 0 0 0 0 0 0 4051 198 1 1 0 0 0 0 4052 200 0 0 0 0 0 0 
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Certificates of Analysis Appendix CC 

1. Feed Certificates of Analysis .....................................................................................586 

2. Water Certificates of Analysis ...................................................................................598 

3. Bedding Certificates of Analysis ...............................................................................609 
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Appendix DD

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211-TBA-1G Final Report

Historical Control Data 

Preface Appendix EE 

1. Huntingdon Life Sciences: CD Rat - Mating, Fertility and Gestation 

Data used for historical control purposes was obtained from inhalation studies run 

prior to this study (from April 2001 to May 2002). 

2. Charles River Pathology Associates: Sprague-Dawley Rat Sperm Analysis 
Huntingdon Life Sciences 00-4206 

211-TBA-1G 

Historical Control Data 

CD Rat - Mating, Fertility and Gestation Ap

Females 

Study Initiation Paired w/ Mated Mating Index Pregnant 

Fertility 

Index 

No. w

Liveb

ID Date Males No. No. % No. % No

00-4208 P0 12-Jun-01 26 24 92.3 23 95.8 200-4208 F1 26 23 88.5 21 91.3 200-4207 P0 30-Apr-01 26 25 96.2 24 96.0 24 00-4207 F1 26 25 96.2 25 100.0 2

00-4205 20-May-02 26 25 96.2 22 88.0 200-4204 19-Mar-02 26 26 100.0 25 96.2 200-4203 21-Jan-02 26 26 100.0 25 96.2 200-4202 22-Aug-01 26 26 100.0 24 92.3 24 

 MEAN 25 96.2 24 94.5 2

SD 1.1 4.10 1.5 3.73 1.

N 8 8 8 8 8 

 MIN 23 88.5 21 88.0 2

 MAX 26 100 25 100 2

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211-TBA-1G Final Report 

 

Testing Facility Personnel Appendix FF 

TITLE/DEPARTMENT NAME/DEGREE 

SENIOR VICE PRESIDENT, 

 SAFETY ASSESSMENT 

Sylvie J. Gosselin, D.V.M., Ph.D., 

 Diplomate A.C.V.P. 

 

SENIOR DIRECTOR, SAFETY 

 ASSESSMENT 

Carol S. Auletta, M.B.A., D.A.B.T., 

 R.A.C. 

 

DIRECTOR, ANALYTICAL SERVICES Barbara A. Litzenberger, B.S., 

 M.T. (ASCP) 

 

DIRECTOR, TOXICOLOGY 

 OPERATIONS Ian Vanterpool, F.I.A.T. 

 

DIRECTOR, QUALITY ASSURANCE Nicki S. Iacono, B.S. 

STUDY DIRECTOR Gary M. Hoffman, B.A., D.A.B.T. 

PATHOLOGIST Katharine M. Whitney, D.V.M., 

 Ph.D., Diplomate A.C.V.P. 

STUDY MONITOR Janet E. Scimone, B.A. 

 

VETERINARIAN Teresa S. Kusznir, V.M.D. 

MANAGER/SUPERVISOR 

 Reproductive and Inhalation Toxicology 

 Analytical Services 

 Histology 

Robert Faust, B.S. 

Kay Saladdin, B.S. 

Janet Kusisto 
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Report Amendments Appendix GG 

There are no amendments for this report at this time.