# STUDY NO. 00

- 4206

SPONSOR STUDY NO. 211

- TBA

- 1G

GASOLINE TBA VAPOR CONDENSATE:

A ONE

- GENERATION WHOLE

- BODY INHALATION REPRODUCTION

TOXICITY STUDY IN RATS

Final Report

Submitted to: American Petroleum Institute

1220 L Street, Northwest

Washington, D.C. 20005

- 4070

Attn: Thomas M. Gray, M.S., D.A.B.T.

Date: 1 September 2011

Page 1 of 652

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Gasoline TBA Vapor Condensate: A One- Generation

Whole-Body Inhalation Reproduction Toxicity Study in Rats

SUMMARY

This study was designed to assess the potential reproductive toxicity of Gasoline TBA

Vapor Condensate when administered via whole

- body inhalation exposures to Sprague

Dawley rats (26/sex/group). The test substance was administered (using a nitrogen

enric hed air mixture) at target concentrations of 2000, 10000 and 20000 mg/m 3 

# for

6 hours/day, 7 days/week for 10 weeks before mating, for up to 2 weeks during mating,

for 3 weeks during gestation and for 4 weeks during lactation prior to weaning. In

addition, a control group (26/sex) received nitrogen enriched air only while in chamber.

Exposure levels were determined using an infra

- red spectrophotometer 4 times per

chamber per day. Additionally, the test substance’s major components were assayed once

per chamber per week. Particle size distribution measurements were also made once per

chamber per week using a TSI Aerodynamic Particle Sizer.

Viability checks were performed twice daily to check for mortality and signs of severe

toxic or pharmacologic effect s. Physical observations and body weight measurements

were made at least once pretest and at least weekly during the study. Feed consumption

measurements were obtained beginning the week prior to treatment initiation and at least

weekly during the study. After completion of ~ 16 weeks of exposures, all parental male

animals (P 0 

# generation) were sacrificed. All parental female animals (P 0 

# generation)

were sacrificed on Lactation Day 28. Selected organs were weighed and organ/body

weight and organ/brain weight ratios calculated. Complete macroscopic postmortem

examinations were performed on all parental animals. Histopathological evaluations of

selected tissues were conducted on randomly selected parental animals.

Pups (F 1 

# generation) were observed as soon as possible after delivery for their sex, the

number of live and dead pups and pup abnormalities. Thereafter, litters were observed

twice daily. On the 4 th 

# day after parturition, litters with more than ten pups were

randomly culled to ten pups with sex distribution equalized when possible. Pups were

given a gross physical examination, were weighed and were sexed frequently until

weaning on the 28 th 

# day after parturition at which time they were sacrificed. A

macroscopic postmortem evaluation and the weighing of selected organs were performed

on selected pups.

The mean ( ± standard deviation) analytical exposure concentrations of Gasoline TBA

Vapor Condensate were determined to be 0 ± 0, 2015 ± 176, 10254 ± 355 and

20382 ± 928 mg/m 3 

# for the Air Contro l and the exposure groups, respectively. The

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SUMMARY

analytically measured exposure levels of the airborne test substance were acceptably

close , in the opinion of the study director, to the targeted exposure levels. The measured

and nominal concentrations varied somewhat (less than 11%), but reasonably, from the

expected 1:1 ratio for this type of vapor exposure. Chamber environmental conditions

averaged 24oC temperature and 45% relative humidity. Particle sizing results indicated

that the atmospheres were esse ntially vapor only, as expected, since there was no

substantial difference between the particulate levels in the test substance chambers and

the Air Control chamber. Analysis of the major components in the neat test substance

and the test atmospheres show ed an acceptably close, in the opinion of the study director,

comparison between the neat test substance and the vaporized test substance. This data

demonstrated that the test animals were exposed, as expected, to all of the major

components of the test s ubstance in their reasonably proper proportion. The data was

consistent from week

- to

- week during the study indicating stability of the test substance

and the atmosphere generation techniques.

There was no effect of treatment on survival, clinical observat ions, body weights and feed

consumption except an exposure

- related decrease in body weight gain was seen in the

20000 mg/m 3 

# animals during the premating period. There was no effect of treatment on

estrous cycle (as measured by cycle length and number of e strous cycles), mating indices

for the male rats and mating or fertility or gestation indices for the female rats except the

animals in the 20000 mg/m3 group took slightly longer to mate than those in the other

groups. There were no treatment

- related differences in the other reproductive parameters

up to the time of parturition including the percent of females completing delivery and the

duration of gestation, when compared to the Air Control group. There were also no

treatment

- related differences in all parturition parameters including the total number of

pups delivered, the number of pups dying, the viability (4 day survival) and lactation

(21 day survival) indices, the number of implantation sites per litter, the pup sex ratio and

the number of live pup s/litter, when compared to the Air Control group. Mean percent

motility, epididymal sperm count, homogenization

- resistant testicular sperm count, sperm

morphology and caudal epididymis weight were not affected by treatment with the test

substance at an ex posure level of 20000 mg/m 3



# .

There were no exposure

- related differences in terminal estrous and macroscopic

postmortem evaluations in the test substance exposed animals compared to the Air

Control animals. Exposure

- related effects on organ weights include d statistically

significant increases in kidney weights (absolute and/or relative to body and brain weight)

at the 2 higher exposure levels in the males. These differences for the males were

consistent with the hydrocarbon nephropathy findings at these exposure levels in the

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SUMMARY

13- week inhalation study with this same test substance. This species - and gender- specific

change has been well documented in male rats exposed to a variety of hydrocarbon

compounds and is not considered relevant to humans. Statistica lly significant decreases

were seen in spleen weights (absolute and/or relative to body and brain weight) at all

exposure levels in the males and females. No other test substance related microscopic

changes were noted in male and female reproductive organ s or other protocol

- specified

tissues in this study.

The pups were generally unremarkable during the lactation period. There were no

exposure- related differences in body weights or weight gains in the pups feeding from

test substance exposed animals in th e 2000 or 10000 mg/m3 groups, compared to the pups

feeding from Air Control animals. Early in lactation, up to about post

- natal day 14, a

slight but statistically significant deficit in pup weight gain occurred at 20000 mg/m3.

There were no exposure

- relate d differences in macroscopic postmortem evaluations in the

pups feeding from test substance exposed animals compared to the pups feeding from Air

Control animals. However, there were exposure

- related decreases in spleen weights in

the pups feeding from the 20000 mg/m 3 

# exposed animals compared to the pups feeding

from Air Control animals.

In conclusion, exposure of rats to 2000, 10000 and 20000 mg/m 3 

# of vapor of Gasoline

TBA Vapor Condensate resulted in decreased body weight gains in the 20000 mg/m 3



# exposed parental animals during the premating interval and decreased spleen weights in

the 2000, 10000 and 20000 mg/m 3 

# exposed parental animals at their termination.

Increases in kidney weights in parental male animals exposed to the 2 higher exposure

levels of vapor were consistent with hydrocarbon nephropathy seen in similar animals

exposed for 13 weeks to this test substance. However, this finding has been generally

accepted (US EPA 1991. Alpha 2 microglobulin: association with chemically induced

renal toxici ty and neoplasia in male rat. In Risk Assessment Forum, p.85. US Govt.

Printing Office, Washington, DC.) not to be relevant to human risk assessment.

Therefore, a no observable adverse effect level (NOAEL) was not determined. There was

no effect at any of the exposure levels on reproductive performance in the study,

including mating, fertility, parturition, lactation and offspring survival except a slightly

extended mating period for the 20000 mg/m 3 

# test substance exposed animals and

decreases in spleen weights in pups feeding from 20000 mg/m 3 

# test substance exposed

animals were seen. Therefore, with respect to reproductive performance, a no observable

effect level ( NOEL) of 10000 mg/m3 of Gasoline TBA Vapor Condensate was also

concluded with an NOAEL of 20000 mg/m 3



# .

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TABLE OF CONTENTS

COVER PAGE .................................................................................................................... .1

STATEMENT OF COMPLIANCE .....................................................................................2

SIGNATURE PAGE ...........................................................................................................3

QUALITY ASSURANCE STATEMENT ..........................................................................4

SUMMARY ....................................................................................................................... ..5

TABLE OF CONTENTS .....................................................................................................8

1. 

# INTRODUCTION ................................................................................................11

2. 

# MATERIALS AND METHODS

2.1. Study Management ....................................................................................11

2.2. Study Dates ................................................................................................11

2.3. Experimental Outline .................................................................................13

2.4. Test Substance ...........................................................................................14

2.5. Test Animals ..............................................................................................15

2.6. Animal Assignment ...................................................................................16

2.7. Animal Identification .................................................................................17

2.8. Veterinary Care ..........................................................................................17

2.9. Animal Husbandry During Non-Exposure Periods ...................................17

2.10. Test Substance Administration and Chamber Operation ...........................20

2.11. Experimental Ev aluations ..........................................................................20

2.12. Mating, Gestation and L actation Procedures .............................................21

2.13. Postmortem ................................................................................................23

2.14. Sperm Count, Motility and Morphology Assessments ..............................27

2.15. Statistical An alysis .....................................................................................28

2.16. Data Storage ...............................................................................................29

2.17. Regulatory References ...............................................................................30

2.18. Protocol Deviations ....................................................................................30

3. 

# RESULTS AND DISCUSSION

3.1. Chamber Monitoring ..................................................................................32

3.2. Parental Data ..............................................................................................33

3.3. Pup Data .....................................................................................................38

3.4. Postmortem Data ........................................................................................39

4. 

# CONCLUSION ....................................................................................................42

REFERENCES ..................................................................................................................43

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TABLE OF CONTENTS

CALCULATIONS .............................................................................................................44

GENERAL PREFACE ......................................................................................................46

TABLES

1. Summary of Survival and Pregnancy ............................................................................... 47

2. Summary of Weekly Clinical Observations - (Premating/Mating/Postmating)) ............ 48

3. Summary of Clinical Observ ations During Gestation ..................................................... 54

4. Summary of Maternal Clinical Ob servations During Lactation ..................................... 55

5. Mean Body Weight Values – (P remating/Mating/Postmating) ...................................... 56

6. Mean Body Weight Gain - (Pre mating /Mating/Po stmating) ......................................... 61

7. Summary of Gestation Body Weights ............................................................................. 67

8. Summary of Gestation Body Weight Gain ...................................................................... 68

9. Summary of Maternal Lactation Body Weights .............................................................. 69

10. Summary of Maternal Lactation Body Weight Gain ....................................................... 70

11. Mean Feed Consumption (grams/kg/ day) - (Premating/Postmating) ............................ 71

12. Summary of Gestation Feed Consumption (grams/kg/day) ........................................... 75

13. Summary of Lactation Feed Consumption (grams/kg/day) ........................................... 76

14. Summary of Estrous Stages .............................................................................................. 77

15. Summary of Cohabitation Data ........................................................................................ 78

16. Summary of Delivery and Litter Data .............................................................................. 79

17. Summary of Pup Clinical Observations During Lactation .............................................. 83

18. Summary of Mean Pup Body Weights ............................................................................ 84

19. Summary of Parental Necropsy Observations ................................................................. 90

20. Summary of Pup Necropsy Observations

Summary of Necropsy Observations for Dead Pups ....................................................... 95

Summary of Necropsy Observations for Stillborn Pups ................................................. 97

Summary of Necropsy Observations for Culled Pups ..................................................... 99

Summary of Necropsy Observations for Cannibalized Pups ........................................ 100

Summary of Necropsy Observations for Weanling Pups .............................................. 101

21. Summary of Parental Organ Weights ............................................................................ 102

22. Summary of Pup Organ Weight Data ............................................................................ 115

23. Lesion Incidence Summary with Expanded Severity Levels ........................................ 119

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TABLE OF CONTENTS

APPENDICES

A. Inhalation Report ......................................................................................................... 125

B. Analytical Report ......................................................................................................... 175

C. Individual Animal Term ination History ..................................................................... 206

D. Individual Weekly Clinical Observations - (Premating/Mating/Postmating) ............ 214

E. Individual Clinical Observa tions During Gestation ................................................... 239

F. Individual Maternal Clinical Ob servations During Lactation .................................... 247

G. Individual Body Weights - (Prema ting/Mating/Postmating) ..................................... 259

H. Individual Body Weight Gain - (P remating/Mating/Postmating) .............................. 275

I. Individual Gestati on Body Weights ............................................................................ 291

J. Individual Gestation B ody Weight Gain .................................................................... 295

K. Individual Maternal Lact ation Body Weights ............................................................ 299

L. Individual Maternal Lacta tion Body Weight Gain ..................................................... 303

M. Individual Feed Consumption (grams/kg/day) - (Premating/Postmating) ................. 307

N. Individual Gestation Feed Cons umption (grams/kg/day) .......................................... 315

O. Individual Maternal Lactation Feed Consumption (grams/kg/day) ........................... 319

P. Individual Estrous Cycl e and Mating Data ................................................................. 323

Q. Individual Mating Assignments .................................................................................. 331

R. Individual Delivery a nd Litter Data ............................................................................ 335

S. Individual Pup Sex and Stat us During Lactation ........................................................ 339

T. Individual Pup Clinical Observ ations During Lactation ............................................ 343

U. Individual Pup Body Weights ..................................................................................... 383

V. Individual Terminal Estr ous Determinations .............................................................. 407

W. Individual Parental Necr opsy Observations ............................................................... 411

X. Individual Pup Necrops y Observations ...................................................................... 434

Y. Individual Parental Organ and Fi nal Body We ights and Ratios ................................ 466

Z. Individual Pup Organ Weights .................................................................................... 495

AA. Individual Animal Gross and Mi croscopic Observations .......................................... 528

BB. Sperm Analysis Report ...........................................................................................571

CC. Certificates of Analysis – (Feed, Water and Bedding) ...........................................585

DD. Protocol and Protocol Amendments ........................................................................... 616

EE. Historical Control Data ............................................................................................... 648

FF. Testing Facility Personnel ........................................................................................... 651

GG. Report Amendments .................................................................................................... 652

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1. INTRODUCTION

This reproductive study was designed to pr ovide general information concerning

the effects of Gasoline TBA Vapor Conde nsate on the integrity and performance

of the male and female reproductive systems, including gonadal function, the

estrous cycle, mating behavior, conception, gestation, parturition and lactation,

and to provide information about the e ffects of the test substance on neonatal

morbidity and mortality, and data on prenatal and postnatal developmental

toxicity.

2. MATERIALS AND METHODS

2.1. STUDY MANAGEMENT

2.1.1. SPONSOR

American Petroleum Institute (API)

1220 L Street, Northwest

Washington, DC 20005-4070

2.1.2. SPONSOR REPRESENTATIVE

Thomas M. Gray, M.S., D.A.B.T.

2.1.3. TESTING FACILITY

Huntingdon Life Sciences (HLS)

P.O. Box 2360

Mettlers Road

East Millstone, New Jersey 08875-2360

2.1.4. STUDY DIRECTOR

Gary M. Hoffman, B.A., D.A.B.T.

2.2. STUDY DATES

2.2.1. STUDY INITIATION

02 August 2002 (Date Study Director signed the Protocol)

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2.2.2. DATE OF ANIMAL RECEIPT

06 August 2002

2.2.3. EXPOSURE INITIATION

20 August 2002 (Experimental Start Date)

2.2.4. MATING INITIATION

28 October 2002

2.2.5. EXPOSURE TERMINATION

28 December 2002

2.2.6. TERMINAL SACRIFICE

P 0 

# Males: 11 – 12 December 2002

P 0 

# Females and F 1 

# Pups: 17 – 29 December 2002

2.2.7. EXPERIMENTAL TERMINATION DATE

31 August 2011 (Date of last data co llection = Date final report is

signed by the Pathologist)

2.2.8. STUDY COMPLETION

1 September 2011 (Date Study Director signed the Final Report)Huntingdon Life Sciences00-4206Page 13211-TBA-1GFinalReport2.3.EXPERIMENTAL OUTLINEGroupGroupDesignationExposureLevels(mg/m3)aNumber of AnimalsMated AdultsMicroscopicPathology AdultGenerationsbMacroscopic PostmortemExaminations-PupsP0P0M F M F F1I Control 0 (air only) 26 26 10 103/sex/litterII Low 2,000 26 26 0 0 3/sex/litterIII Middle 10,000 26 26 0 0 3/sex/litterIV High 20,000 26 26 10 10 3/sex/litteraExposures daily (7 days/week) for 6 hours per day.bHistologic examinations were performed on reproductivetissues for the control and high-exposure animals.M = Male; F = Female; The first day of exposure was Day 0.

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2.4. TEST SUBSTANCE

Gasoline TBA Vapor Condensate

2.4.1. SUPPLIER

Chevron Global Technology Services Company

100 Chevron Way

Richmond, CA 94802

2.4.2. LOT NUMBER

API 01-07

2.4.3. PURITY/ANALYTICAL CONCENTRATION

100% Gasoline TBA Vapor Condensate

2.4.4. DESCRIPTION

Colorless Liquid

2.4.5. DATES RECEIVED

18 March 2002 and 05 August 2002

2.4.6. EXPIRATION DATE

Not available, stable per MSDS. The test substance stability was

tested concurrently with the st udy and the results (see Appendix B)

showed it to be stable for the duration of the study.

2.4.7. ANALYSIS

Documentation of the identity, strength, composition, and method

of synthesis, fabrication and/or de rivation of the batch of the test

substance and the maintenance of these records was the

responsibility of the Sponsor. The Sponsor conducted a purity

analysis of the test substance by GC prior to the start of this study.

2.4.8. STORAGE

Ambient conditions in an outdoor solvent shed or in an indoor

laboratory.

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2.4.9. DISPENSING

The test substance was received from Chevron Global Technology

Services Company in 6 of the 100 gallon cylinders. Since only 5

gallon cylinders were practical to be used for exposure operations,

the test substance was dispensed, as needed, at the Testing Facility

from the 100 gallon cylinders into 5 gallon cylinders using

nitrogen pressurization.

2.4.10. ARCHIVAL SAMPLE

A 50 mL sample from the lot of test substance is stored in the

Archives of the Testing Facility under ambient conditions in an

indoor laboratory.

2.4.11. DISPOSITION

The unused portion of the test substa nce, as well as any empty test

substance containers, were returned to the Sponsor. Empty test

substance containers also were returned to the Sponsor on an as

needed basis. The Sponsor was responsible for tracking their

disposition.

2.5. TEST ANIMALS

Albino Rats (Outbred) VAF/Plus 



# 2.5.1. SPECIES

Sprague-Dawley derived CD ®



# [Crl: CD ® 

# IGS BR]

2.5.2. SUPPLIER

Charles River Laboratories

Kingston, New York 12484

2.5.3. JUSTIFICATION FOR TEST ANIMAL SELECTION

The rat was used as a surrogate for humans in the detection of

reproductive effects and is a species in which known reproductive

toxicants have been detected. The rat is a rodent animal model

commonly utilized in reproduction studies and is recommended in

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the referenced regulatory guidelines. In addition, a historical

control database with this strain of animal and supplier facility is

available for comparative evaluation.

2.5.4. NUMBER OF ANIMALS

Received:

220 total (110 males, 110 females)

Placed on test:

208 total (104 males, 104 females)

Females were nulliparous and non-pregnant.

2.5.5. AGE AT RECEIPT

Approximately 28 days.

2.5.6. AGE AT INITIATION OF EXPOSURES

Approximately 42 days.

2.5.7. WEIGHT AT INITIATION OF EXPOSURES (GRAMS)

Mean Range

Male: 183 160-209

Female: 167 146-191

2.5.8. ACCLIMATION PERIOD

Animals were acclimated for 14 days. All animals were checked

for viability twice daily. All animals were examined during the

acclimation period to confirm suitability for study.

2.6. ANIMAL ASSIGNMENT

More animals than required for the study were purchased and acclimated.

Animals considered suitable for the study on the basis of pretest physical

examinations and body weight data were randomly assigned, by sex, to

control or treated group, in an attempt to equalize mean group body

weights. Individual weights of anim als placed on test did not exceed 20%

of the mean weight for each sex. Di sposition of all animals not utilized in

the study is maintained in the study file.

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2.7. ANIMAL IDENTIFICATION

Each rat was assigned a temporary identification number upon receipt.

After selection for study (P 0 

# generation), each rat was ear-tagged with a

number assigned by the Testing Facility. This assigned animal number

plus the study number comprised the unique animal number for each

animal. If the tag was lost, it was replaced. In addition, each cage was

provided with a cage card that was color-coded for exposure level

identification and contained the study number and animal number

information.

2.8. VETERINARY CARE

Animals were monitored by the t echnical staff for any conditions

requiring possible veterinary care.

2.9. ANIMAL HUSBANDRY DURING NON-EXPOSURE PERIODS

2.9.1. FACILITIES MANAGEMENT/ANIMAL HUSBANDRY

Currently acceptable practices of good animal husbandry were

followed e.g., Guide for the Care and Use of Laboratory Animals ;

National Academy Press, 1996. Huntingdon Life Sciences, East

Millstone, New Jersey is fully accredited by the Association for

Assessment and Accreditation of Laboratory Animal Care

International (AAALAC).

2.9.2. HOUSING

Animals were housed in suspended, stainless steel cages with wire

mesh fronts and floors. Animals were doubly housed during the

initial week of acclimation and individually housed thereafter,

except as follows.

Mating: One male and one female were co-housed continuously

(except during treatment) until mating occurred or for a maximum

of 14 days. Mating pairs were assigned randomly.

Lactation: Dam and litter were housed together in a solid plastic

“shoebox” cage until weaning.

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2.9.3. FEED

Certified Rodent Diet, No. 5002; (Meal) (PMI Nutrition

International, St. Louis, Missouri) was available without restriction

except during exposures.

2.9.4. FEED ANALYSIS

Analysis of each feed lot used during this study was performed by

the manufacturer. Results can be found in Appendix CC. There

were no known contaminants in the feed which were expected to

interfere with the results of this study.

2.9.5. WATER

Water (Elizabethtown Water Company, Westfield, New Jersey)

was available without restriction, except during exposures, via an

automated watering system.

2.9.6. WATER ANALYSIS

Water analyses are conducted by Elizabethtown Water Company,

Westfield, New Jersey (Raritan-Millstone Plant) to ensure that

water meets standards specified under the EPA Federal Safe

Drinking Water Act Regulations (40 CFR Part 141). In addition,

water samples are collected biannually from representative rooms

in the Testing Facility; chemical and microbiological water

analyses are conducted on these samples by a subcontract

laboratory (Lancaster Laboratories, La ncaster, PA). Results of all

water analyses can be found in Appendix CC. There were no

known contaminants in the water that were expected to interfere

with the results of this study.

2.9.7. BEDDING SUBSTANCE

Ground corncob bedding (Bed-O’-Cobs ® 

# 1⁄4 inch irradiated, The

Anderson’s Inc., Maunee, OH), was provided for each mated

female on Day 18 of gestation. Fresh bedding was provided at

least weekly and as needed until Lactation Day 28.

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2.9.8. BEDDING ANALYSIS

Analyses for a representative ba tch of bedding as used on study

can be found in Appendix CC. There were no known

contaminants in the bedding that were expected to interfere with

the results of this study.

2.9.9. ENVIRONMENTAL CONDITIONS

Light/Dark Cycle

A twelve-hour light/dark cycle controlled by an automatic timer

was provided.

Temperature

Temperature was monitored in accordance with Testing Facility

SOPs and maintained within the specified range to the maximum

extent possible. Excursions out side the specified range were

generally brief in duration and cons idered not to have affected the

integrity of the study.

Desired: 19 to 25 ° C

Actual: 14.3 to 26.0 ° C

Average: 17.7 to 24.1 ° C

Relative Humidity

Relative humidity was monitored in accordance with Testing

Facility SOPs and maintained with in the specified range to the

maximum extent possible. Excursions outside the specified range

were generally brief in duration and considered not to have

affected the integrity of the study.

Desired: 30 to 70%

Actual: 14.26 to 99.81%

Average: 23.46 to 75.42%

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Air Changes

Air changes were maintained at a rate of 10-15 per hour range to

the maximum extent possible and were recorded once prior to

exposures.

Desired: 10 to 15 per hour

Actual: 11.5 to 14.6 per hour

2.10. TEST SUBSTANCE ADMINISTRATION AND CHAMBER

OPERATION

Inhalation (whole-body exposures).

Details of test article administration and chamber operation information

are presented in the Inhalation Report (Appendix A).

2.11. EXPERIMENTAL EVALUATIONS

2.11.1. VIABILITY EXAMINATION (CAGESIDE)

Observations for mortality and signs of severe toxic or

pharmacological effects were made at least twice daily (morning

and afternoon).

2.11.2. DETAILED PHYSICAL EXAMINATIONS

The animals were observed as a group at least once during each

exposure.

In addition, each parental animal was removed from its cage and

examined at least once pretest, at the study start (first day of

treatment), and weekly thereafter during the study period. Females

were then observed weekly until there was evidence of mating.

Once mated, females were observed on Gestation Days 0, 7, 14,

and 20 and on Lactation Days 1, 4, 7, 14, 21, and 28. Females that

showed evidence of mating but did not deliver were observed on

Gestation Days 0, 4, 7, 14, and 20 and were euthanized on

Gestation Day 25. Females w ithout evidence of mating were

observed weekly until euthanized on the last possible Gestation

Day 25. Examinations included, but were not limited to,

observations of general condition, skin and fur, eyes, nose, oral

cavity, abdomen and external genitalia, as well as evaluations of

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respiration and palpation for tissue masses. Physical examinations

were performed post-exposure except during the gestation and

lactation periods when they were performed pre-exposure.

2.11.3. BODY WEIGHT

Each parental animal was remove d from its cage and weighed at

least once pretest, at the time of randomizing into study groups, on

the day treatment initiated, and weekly thereafter during the study

period (including the mating period) and prior to termination.

Once mated, females were weighed on Gestation Days 0, 4, 7, 14,

and 20 and on Lactation Days 1, 4, 7, 14, 21, and 28. Females that

did not show evidence of mating a nd/or did not deliver pups were

weighed weekly until euthanasia.

2.11.4. FEED CONSUMPTION

Feed consumption was measured once pretest and weekly

thereafter during the study period. Once mated, female feed

consumption was measured on Gesta tion Days 0, 4, 7, 14, and 20

and on Lactation Days 1, 4, 7, 14, 21, and 28 until euthanasia.

Females without evidence of mating and/or that did not deliver

pups had food consumption measured weekly until euthanasia.

Feed consumption was not measured for any animal during the

mating period when males and females were co-housed.

2.12. MATING, GESTATION AND LACTATION PROCEDURES

2.12.1. ESTROUS CYCLING

Daily vaginal smears were taken at approximately the same time

each day, and the stage of estrus was determined for each P 0 

# female beginning three weeks prior to cohabitation and continuing

until there was evidence of mating or until the 14-day mating

period ended. Care was taken to reduce the induction of pseudopregnancy.

2.12.2. MATING PROCEDURE

Following a 10-week pre-mating treatment period, one male and

one female (non-siblings) from the same exposure group were

caged together until evidence of mating was observed (a

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copulation plug in the vagina and/or microscopic observation of

sperm in the vaginal smear) or 14 consecutive days had elapsed.

Males and females were not caged together during the mating

period while being exposed. The day evidence of mating was

observed was defined as Day 0 of gestation. If mating did not

occur after 14 days, the animals were separated without further

opportunity for mating.

2.12.3. PARTURITION AND LACTATION

On Day 18 of gestation, several da ys prior to expected parturition,

each female was transferred to a solid plastic “shoe-box” cage.

Bedding substance was provided and changed at least weekly and

as needed. Examinations for signs of parturition were made twice

daily (morning and afternoon). The day on which parturition was

first observed was defined as Day 0 of lactation. Females that

exhibited no evidence of mating were transferred to plastic “shoebox” cages when the first animal s mated reached their Day 18 of

gestation.

2.12.4. LITTER EVALUATIONS (F 1 

# )

Observations

F 1 

# litters were observed as soon as possible after delivery for the

number of live and dead pups, sex of the pups and pup

abnormalities. The sex was determined on the day of delivery

completion for all pups by inspection of the ano-genital distance.

All pups were uniquely identified within the litter by toe tattoo on

the day of delivery completion. Thereafter, litters were observed

twice daily (morning and afternoon) for the presence of dead pups

which were removed for examination. Litter size was recorded

daily from Lactation Day 1 to 28.

Culling of Pups

On Day 4 of lactation, each litte r with more that 10 pups was

culled to that number, with sex distribution equalized (five/sex)

when possible. The pups were culled randomly. Preferential

culling of runts was not performed.

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Physical Examinations

Each F 1 

# pup was given a physical examination on the day of

delivery completion and on Day 4, 7, 14, 21 and 28 of lactation.

Body Weight

Individual F 1 

# pup weights were recorded on Day 1, 4 (precull

intervals), 7, 14, 21 and 28 of lactation (postcull intervals).

Sexing

The sex of each pup was recorded on the day of delivery

completion, then reconfirmed on Lactation Day 4, 7, 14, 21, and

28.

2.13. POSTMORTEM

2.13.1. PARENTAL ANIMALS (P 0 

# )

Necropsy was performed on all 26 animals/sex/group. All P 0



# males were euthanized after the last F 1 

# litters were delivered. All

P 0 

# females with litters were euthanized on Day 28 of lactation. P 0 

# females with no confirmed day of mating were euthanized on the

last possible Gestation Day 25. P 0 

# females with confirmed days of

mating that did not deliver were euthanized on Gestation Day 25.

Method of Euthanasia

Exsanguination following anesthesia with inhaled carbon dioxide.

Macroscopic Examination

Complete macroscopic postmortem examinations were performed

on all adult animals, including an imals found dead and dams with

total litter loss. All abnormal obs ervations were recorded. The

necropsy of the parental animal s included examination of the

external surface and all orifices; th e external surfaces of the brain

and spinal cord; the organs and ti ssues of the cranial, thoracic,

abdominal and pelvic cavities and neck; and the remainder of the

carcass. Examination of all P 0 

# females included a vaginal smear to

determine the stage of the estrous cycle and a count of uterine

implantation scars, if present.

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Organ Weights

Organs indicated in Table I (page 25) were weighed for all adult

animals at the scheduled sacrifice intervals, except for dams which

did not produce a litter or had total litter loss. Prior to weighing,

the organs were carefully dissected and properly trimmed to

remove adipose and other contiguous tissues in a uniform manner.

Organs were weighed as soon as possible after dissection in order

to avoid drying. Paired organs were weighed together (gonads

were also weighed separately). Organ weight data are presented as

absolute values and relative to terminal body weight and brain

weight.

Tissues Preserved and Examined Histopathologically

The tissues listed in Table I (p age 25) were obtained at the

scheduled sacrifice intervals and preserved for all animals, except

for dams which did not produce a litter or had total litter loss.

Slides of the indicated tissues were prepared and examined

microscopically for 10 randomly selected animals in the control

and high exposure groups.

Histopathological examinations of the testes were conducted to

identify potential treatment-related effects such as retained

spermatids, missing germ cell layers or types, multinucleated giant

cells, or sloughing of spermatogenic cells into the lumen. The

examination of the intact epididym is was of a longitudinal section

that permitted examination of the caput, corpus and cauda regions,

to identify such lesions as sperm granulomas, leukocytic

infiltration (inflammation), aberrant cell types within the lumen, or

the absence of clear cells in the cauda epididymal epithelium.

Histopathological examination of the ovary included evaluation of

five sections taken at least 100 μ m apart from the inner third of

each ovary. These examinations were to detect depletion of the

primordial follicle population and enumerated the total number of

primordial follicles for comparison with the ovaries from control

animals. These examinations were also to confirm the presence or

absence of growing follicles and corpora lutea in comparison to

control ovaries.

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TABLE I

ORGAN NAME WEIGHED PRESERVED EXAMINED

MICROSCOPICALLY

(Groups I and IV)

adrenal glands X X X

brain (medulla, pons, cerebrum and

cerebellum) X X

epididymides (total and caudal) X X a 

## X a



## kidneys X X

liver X X

lungs (with mainstem bronchi) X X X

ovaries X X X

pituitary gland X X X

prostate gland X X X

seminal vesicles with coagulating glands

(and fluids) X X X

spleen X X

testes X X a 

## X a



## uterus (body/horns) with cervix and

oviducts X X X

vagina X X

tissues with macroscopic findings

including tissue masses X X

target organs X X

a 

## Right testis and epididymis only unless a macroscopic abnormality was noted on the left testis or left

epididymis, then the left testis and left epididymis were preserved instead for possible histopathology.



# Preservatives

All tissues - 10% neutral buffered formalin (NBF).

Testes and epididymides were placed in Modified Davidson’s

solution initially and then retained in 10% formalin. Lungs were

infused with formalin prior to their immersion into a larger volume

of the same fixative.

Processing

After fixation, the tissues and organs from randomly selected

animals (10/sex/Groups I and IV) were routinely processed,

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embedded in paraffin, cut at a microtome setting of 4-7 microns,

mounted on glass slides and stained with hematoxylin and eosin.

2.13.2. F 1 

# PUPS AND WEANLINGS

Method of Euthanasia

F 1 

# pups (on Lactation Day 4) were euthanized via an

intraperitoneal injection of sodium pentobarbital. F 1 

# pups (after

Lactation Day 4) and weanlings were euthanized by

exsanguination after anesthesia with inhaled carbon dioxide.

Dead and Culled Pups

F 1 

# pups found dead at birth were identified as stillborn or born

alive but found dead (lung floatation test). F 1 

# pups found dead

during the lactation period were examined to the maximum extent

possible for defects and/or the cause of death, and for the presence

or absence of milk in the stomach. Dead pups were preserved

intact in 10% NBF. Partially cannibalized pups were examined to

the maximum extent possible and discarded. Culled F 1 

# pups were

examined for external abnormalities. If unremarkable, these pups

were then euthanized and discarded. Culled F 1 

# pups with external

abnormalities were preserved intact in 10% NBF.

Terminal Necropsy:

Macroscopic Examination and Tissues Preserved

Macroscopic examinations were performed on up to 3 randomly

selected pups/sex/litter on Day 28 of lactation. Examinations

included observations of any structural abnormalities or

pathological changes. Special atte ntion was given to the organs of

the reproductive system, brain, liver, kidneys, pituitary, adrenal

gland, spleen, heart and thymus gland.

All remaining F 1 

# pups were examined for external abnormalities

and euthanized. Pups with external abnormalities were preserved

intact in 10% NBF. Pups with no external abnormalities were

discarded. No further macroscopic postmortem examinations were

performed.

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Organ Weights

The following organs were weighe d from one randomly selected

pup/sex/litter sacrificed on Day 28 of lactation:

brain

spleen

thymus gland

Organ weight data are presented as absolute values and relative to

terminal body weight and brain weight.

2.14. SPERM COUNT, MOTILITY AND MORPHOLOGY

ASSESSMENTS

All P 0 

# males euthanized at termination in each group had the following

sperm evaluations available for analysis: 1) motility; 2) a count of

homogenization-resistant testicular sperm; 3) a count of caudal epididymal

sperm; and 4) sperm morphology (cauda epididymis). Evaluations were

performed by Pathology Associates International, Frederick, MD (PAI) as

follows:

The right testis and right epididym is from each animal were removed

intact, weighed (testes weighed together and also separately) and fixed in

modified Davidson’s solution for at least 48 hours prior to permanent

storage in 10% NBF.

The left epididymis was removed int act, weighed, and frozen on dry ice

for transport to PAI. The epididymides were stored frozen at -70 ° C until

evaluation for caudal sperm count. Each epididymis was thawed and the

caudal portion removed and weighed. A homogenized sample of the

caudal epididymis was stained and then examined using the Hamilton

Thorne IVOS sperm analyzer. For each stained preparation, 20 fields

were counted. The total number of sperm in the caudal epididymis was

calculated and adjusted for the cauda l epididymal weight. Additionally,

for each male, two sperm morphology slid es were prepared, stained with

Eosin and evaluated for morphological development.

Note: If a macroscopic abnormality was noted on the left testis or left

epididymis, then the right epididymis was transferred to PAI and the left

testis and left epididymis was preserved as indicated for possible

histopathology.

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The left vas deferens was excised a nd placed in a prewarmed solution of

phosphate buffered saline and 1% B ovine Serum Albumin. After a

minimum three minute “swimout” period, a sample was placed in a

Hamilton Thorne IVOS sperm analyzer . Five fields were selected and

stored as digital images. The images were analyzed for percent motility

and transferred to optical media for permanent storage.

The left testis was removed and frozen on dry ice for transport to PAI.

The testis were stored frozen at -20 ° C until processed for counting of

homogenization-resistant sperm.

(See Appendix BB for details and methodology.)

Tissues retained from these evaluations will be discarded following

issuance of the final report following consultation with the Sponsor. The

Testing Facility was responsible for the GLP compliance of this

subcontractor.

2.15. STATISTICAL ANALYSIS

2.15.1. CONTINUOUS DATA

The following parameters were analyzed statistically:

Body weights

Body weight change

Feed consumption values

Organ weight data

Estrous cycle data

Gestation length

Pup body weights

Number of pups (live, dead, total)

Method of Analysis

Mean values of all exposure groups were compared to the mean

value for the control group at each time interval.

One-way analysis of variance (ANOVA) was applied (Dunlap and

Duffy, 1975). If a significant F-ratio indicated inequality of group

means, Dunnett’s test (Dunlap et al., 1981) was applied to

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determine which group(s), if a ny, differed significantly from

control.

All tests were conducted at the 5% and 1% significance levels.

2.15.2. SPERM AND MOTILITY ANALYSIS

The following parameters were analyzed statistically:

Mean sperm count (homogenization-resistant testicular sperm

count and caudal epididymal sperm count) and motility data

.



# A Kruskal-Wallis non-parametric ANOVA test was performed to

assess significance (Kruskal and Wallis, 1952, 1953). If a

significant difference occured (p < 0.05), the Wilcoxon (MannWhitney U) test (Kruskal, 1957) was used for pair-wise

comparisons of each treated group to the vehicle control group.

Statistics were performed using an IBM compatible computer with

SAS computer programs (SAS/STAT User’s Guide, 1989).

2.15.3. INCIDENCE DATA

The following parameters were analyzed statistically:

Mortality rates

Mating indices (male and female)

Pregnancy rates

Male fertility indices

Live birth indices

Pup viability indices (Days 0-4) and lactation indices (Days 4-28)

Incidence Data Analysis

A Fisher Exact Test with Bonfe rroni correction was performed to

identify differences between the groups (Siegel, 1956). All

statistical tests were conducted at the 5% and 1%, two-sided risk

levels.

2.16. DATA STORAGE

All raw data, preserved specimens, and retained samples, as well as the

original study protocol and the original final report are to be maintained in

the Archives of the Testing Facility upon completion of the study.

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All raw data and all reports generated by Pathology Associates

International (PAI), Frederick, MD were shipped to Huntingdon Life

Sciences for archival storage.

The Sponsor will determine the final disposition of these materials.

2.17. REGULATORY REFERENCES

2.17.1. TEST GUIDELINE

This study was designed to meet or exceed the pertinent

requirements of:

US EPA Vehicle Emissions Inha lation Exposure Guideline 79.61,

CFR Vol. 59, No. 122, 27 June 1994.

US EPA (Environmental Protection Agency) Health Effects Test

Guidelines, OPPTS 870.3800, Reproduction and Fertility Effects

(August 1998) except this study will only continue through the 1st

generation of offspring.

2.17.2. GOOD LABORATORY PRACTICES

This study was conducted in accordance with US EPA 79.60, CFR

Vol. 59, No. 122, 27 June 1994 and was performed according to

the protocol and Huntingdon Li fe Sciences’ and Pathology

Associates International’s Standard Operating Procedures.

2.17.3. ANIMAL WELFARE ACT COMPLIANCE

This study complied with all appropriate parts of the Animal

Welfare Act regulations: 9 CFR Parts 1 and 2 Final Rules, Federal

Register, Volume 54, No. 168, August 31, 1989, pp. 36112-36163

effective October 30, 1989 and 9 CFR Part 3 Animal Welfare

Standards; Final Rule, Federal Register, Volume 56, No. 32,

February 15, 1991, pp. 6426-6505 effective March 18, 1991.

2.18. PROTOCOL DEVIATIONS

The following protocol deviations o ccurred during the study but were not

considered to have compromised the validity or integrity of the study:

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1. Due to a faulty timer, the twelve-hour light/dark cycle was interrupted

from 21-Oct-02 to 24-Oct-02 with th e result that the lights did not go

off during those three nights. Only a slight but transient effect on

body weights was noted and no appare nt effect on the estrous cycle

was noted.

2. Due to technician error, Animal Number 1535 was not sacrificed on

23 November 2002 (Gestation Day 25) as per protocol. The animal

was sacrificed on 24 November 2002 (Gestation Day 26).

3. Due to technician error, Dam Nu mber 3528’s Pup Number 5 was not

preserved in 10% NBF after necropsy was performed.

4. Due to technician error, the left epididymis for Animal Number 3029

was lost during necropsy on Study Day 113. As a result, the weight

for left epididymis and total orga n weight for this organ was not

recorded.

5. Due to technician error, no vagina l smear was taken prior to necropsy

of Animal Number 1527 which was found dead on 24 November 2002

(Lactation Day 1).

6. Although required in the protocol, an alyses for each batch of bedding

used on study was not provided by the Supplier and therefore not

maintained on file at the Testing Facility. However, representative lot

numbers of bedding which were analyzed and provided by the

Supplier are maintained on file.

7. Due to technician error, for Dam Number 1529, a body weight and

physical observation were not recorded on Lactation Day 1.

8. Due to technician error, the Week 15 (Study Day 105) observations

were not recorded for unmated female animal numbers 1548, 1550,

2527, 2538, 2542, 3538, 3539, 3550 and 4541).

9. Group 1 animals were removed from the whole-body exposure

chamber after 20 minutes post-exposur e on Day 5 instead of after at

least 30 minutes.

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3. RESULTS AND DISCUSSION

3.1. CHAMBER MONITORING

(Appendices A and B)

Chamber distribution analyses (see Tabl e IV in Appendix A) showed that

the test substance was evenly distri buted within each chamber. Chamber

monitoring (see Table VI in Appendix A) showed that the chamber oxygen

levels were at least 19%. Chambe r room monitoring (see Table VI in

Appendix A) showed that no test substance was present in the room and that

the sound and light levels were acceptable.

The target and mean (± standard de viation) analytical (IR) and nominal

concentrations are summarized as follows:

Group Test

Substance Target

Concentration

(mg/m 3 

## ) Analytical

Concentration

(mg/m 3 

## ) Nominal

Concentration

(mg/m 3 

## )

I Air Control 0 0 ± 0 0 ± 0

II Gasoline TBA

Vapor Condensate 2,000 2015 ± 176 2220 ± 113

III Gasoline TBA

Vapor Condensate 10,000 10254 ± 355 10443 ± 294

IV Gasoline TBA

Vapor Condensate 20,000 20382 ± 928 19985 ± 864



# The analytically measured exposure levels of the airborne test substance

were reasonably close to the targeted exposure levels. The measured and

nominal concentrations varied somewhat (less than 11%), but acceptably, in

the opinion of the study director, from the expected 1:1 ratio for this type of

vapor exposure. Chamber environmental conditions averaged 24oC

temperature and 45% relative humidity.

Mean particle size distribution measurements for the exposures are

summarized as follows:

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Group Test

Substance Mass Median

Aerodynamic

Diameter

(μm) Geometric

Standard

Deviation Total

Mass

Concentration

(mg/m 3 

## )

I Air Control 1.713 1.797 5.20 x 10 -3



## II Gasoline TBA

Vapor Condensate 1.792 1.785 5.12 x 10 -3



## III Gasoline TBA

Vapor Condensate 1.642 1.788 5.72 x 10 -3



## IV Gasoline TBA

Vapor Condensate 2.466 1.881 5.44 x 10 -3



# These results indicated that the atmos pheres were essentially vapor only, as

expected, since there was no substantial difference between the test

substance chambers and the Air Control chamber.

Analysis of the major components in the neat test substance and the test

atmospheres (see Appendix B) showed an acceptably close, in the opinion

of the study director, comparison between the neat test substance and the

vaporized test substance. This data demonstrated that the test animals were

exposed, as expected, to all of the majo r components of the test substance in

their proper proportion. The data were consistent from week-to-week

during the study indicating stability of th e test substance and the atmosphere

generation techniques.

3.2. PARENTAL DATA (P 0 

# GENERATION)

3.2.1. MORTALITY

(Table 1, Appendix C)

There was no effect of treatment on survival. All animals survived

until their scheduled termination except:

1. Female #4549 of the 20000 mg/m 3 

# group that was found dead

on study day 39. This animal ha d no prior clinical signs or body

weight effects but was noted at necropsy with a broken incisor,

discolored thymus and discolored lungs.

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2. Female #1527 of the Air Control group that was found dead on

lactation day 1. This animal had no prior clinical signs or body

weight effects but was noted at n ecropsy with a discolored thymus,

discolored adrenals and a fetus retained in the left uterine horn.

3. Female #2549 of the 2000 mg/m3 group and females # 3536 and

3548 of the 10000 mg/m3 group after total litter loss on study day

119.

3.2.2. PHYSICAL OBSERVATION DATA

(Tables 2, 3 and 4; Appendices A, D, E and F)

The test animals were unremarkable in-chamber during the

exposure periods.

An increased incidence of alopecia of the extremities/snout was

seen in the males at 10000 and 20000 mg/m3. Otherwise, the test

animals were generally unremarkable during the non-exposure

periods during the premating period in both sexes, the

mating/postmating period in the male rats, and the gestation and

lactation periods in the female rats. Scattered observations such as

chromodacryorrhea were noted but were considered not treatmentrelated.

3.2.3. BODY WEIGHTS – PREMATING PERIOD (BOTH SEXES)

AND PREMATING/POSTMATING PERIOD (MALES)

(Tables 5 and 6; Appendices G and H)

Over approximately the first 3 weeks of exposure at 20000 mg/m3,

especially the males and to a lesser extent the females showed

statistically significantly reduced weight gain, resulting in an

approximate 6% deficit in body weight as compared with Control.

This deficit subsequently remained until termination for the males

and into late lactation for the females (see section 3.2.5 below).

Other transient weight gain differences from Control that occurred

sporadically in all the test substance exposed groups were

considered not exposure-related.

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3.2.4. MATERNAL BODY WEIGHTS - GESTATION AND

LACTATION PERIODS

(Tables 7, 8, 9 and 10; Appendices I, J, K and L)

The deficit in female body weight at 20000 mg/m3 (approximately

5% as compared with Control) that was noted during the premating period persisted through gestation and into lactation (~3-

5%), but apparently began to resolve after about post-natal day 14.

There was no difference in body weight gains among groups

during gestation. Body weight gains were significantly increased

in the 20000 mg/m 3 

# group for the entire lactation period (days 1-

28).

3.2.5. FEED CONSUMPTION – PREMATING PERIOD

(Table 11; Appendix M)

There were no exposure-related differences in feed consumption in

the test substance exposed animal s compared to the Air Control

animals. While a few statistically significant differences were

noted at various intervals, the absolute differences were generally

small.

3.2.6. MATERNAL FEED CONSUMPTION - GESTATION AND

LACTATION PERIODS

(Tables 12 and 13; Appendices N and O)

There were no exposure-related differences in feed consumption

during the gestation and lactation periods in the test substance

exposed animals compared to th e Air Control animals. While

statistically significant decreases were noted at two intervals

during the lactation period in the 2000 mg/m 3 

# group, these were

not considered treatment-related.

3.2.7. ESTROUS CYCLE DATA

(Table 14; Appendix P)

There was no effect of treatment on estrous cycle (as measured by

cycle length and number of estrous cycles) for the female rats.

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During the pre-mating period, 16 fema les in the study (3 in the Air

Control group, 4 in the 2000 mg/m3 group, 8 in the 10000 mg/m3

group and 1 in the 20000 mg/m3 group) commenced extended

periods of diestrus (10 days or greater), indicative of incidental

pseudopregnancy. One of these fe males (1529) in the Air Control

group showed persistent diestrus for 15 days (10 days premating

and 15 days cohabitation). One of these females (3550) in the

10000 mg/m3 group showed persistent diestrus for 24 days (10

days premating and 14 days cohabitation). These females showed

no signs of mating and were not pregnant. It is possible that these

animals were “triggered” into sequential periods of

pseudopregnancy.

During the mating period, one female in the Air Control group

(1548) and one female in the 20000 mg/m3 group (2547) became

pseudopregnant at the start and did not recommence normal

cycling before the end of the 2-week mating period. One other

female in each of the 2000 mg/m3 (2538) and 10000 mg/m3 (3539)

groups showed evidence of pseudopregnancy during the latter half

of the mating period, but these 2 females nevertheless had 2

opportunities to mate (2 estruses with the male). One female in the

2000 mg/m3 group (2542) went into a persistent estrus state during

the mating period (having previously shown one normal estrus

with the male, without mating) an d did not become pregnant. One

female in the 20000 mg/m3 group (4541) during the mating period

showed prolonged estrus interspersed with brief diestrus states,

and again no pregnancy resulted.

Of the 16 females that were acyclic during the premating phase, 13

mated with 11 becoming pregnant. The pattern of incidence of

irregular or abnormal cycles acro ss the study was considered not to

indicate an effect of treatment. Adequate assessment of cyclicity

was possible despite the incidental episodes of pseudopregnancy.

3.2.8. MATING, FERTILITY AND GESTATION INDICES

(Tables 15 and 16; Appendices Q and R)

Mating indices for the male rats treated with the test substance

were comparable to the Air Control group. Mating, fertility and

gestation indices for the female rats treated with the test substance

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were comparable to the Air Control group. The animals in the

20000 mg/m3 group took slightly longer to mate than those in the

other groups. The Mating, Fertility , and Gestation Indices in each

group were as follows:

Parameter Air

Control 2000

mg/m 3 

## 10000

mg/m 3 

## 20000

mg/m 3



## Number Cohabitated 26 26 26 25

Number Mated 24 23 23 23

Number of Females Missing First Opportunity to Mate 2 3 3 7

Number of Females Missing Two or More Opportunities

to Mate 1 3 2 1

Number Pregnant 21 22 22 22

Number Pregnant with Total Litter Loss 0 1 2 0

Mating Index 92.3% 88.5% 88.5% 92.0%

Fertility Index 87.5% 95.7% 95.7% 95.7%

Gestation Index 100% 100% 100% 100%

Premating Treatment Period

Number of Irregular Cycles1 7 10 6 11

Number of Acyclic Intervals2 3 4 8 1

Cohabitation Treatment Period

Number of Irregular Cycles 0 2 0 1

Number of Acyclic Intervals 1 1 3 0

Outcome of Females with Acyclic Intervals

Number of Females with Persistent Estrus3 0 1 0 1

Total Number of Females with Acyclic Intervals 4 6 10 1

Number of Previously Acyclic Females that Mated 3 5 8 1

Number of Previously Acyclic Females Becoming

Pregnant 3 3 5 0

1Irregular Cycles - Cycles of 2 to 3 days or 6, 7, 8 or 9 days

2Acyclic Intervals - Persistent diestrus of 10 or greater days

3Persistent Estrus - At least 4 consecutive days of estrus



# The apparent similar trend in the 10000 mg/m3 group, indicated by

the number of pairings exceeding 4 da ys to mate, was an artifact of

the pattern of (incidental) pseudopregnancy in the group. There

were no treatment-related differences in the other reproductive

parameters up to the time of part urition, including the duration of

gestation, when compared to the Air Control group.

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3.2.9. PARTURITION AND LITTER SURVIVAL DATA

(Table 16; Appendices R and S)

Parturition data for the female rats treated with the test substance

were comparable to the Air Control group. There were no

exposure-related differences in the number of conceptuses (as

indicated by implantation sites), the number of pups delivered, the

live litter size or the pup sex ratio. The slightly reduced group pup

viability to post-natal day (PND) 4 at 10000 and 20000 mg/m3 was

a result of 2 total litter losses at 10000 mg/m3 and one litter at

20000 mg/m3 that lost 7 of 17 pups, occurrences that were

considered incidental and not rela ted to exposure. The number of

stillborn were significantly increased in the 10000 mg/m3 group.

This increase in the number of stillborn, related to the two dams

with total litter loss, was considered incidental and not related to

exposure since there was one dam in the 2000 mg/m3 group that

also had total litter loss. The to tal litter losses were considered

incidental since they did not occu r in an exposure-related pattern.

Overall, pup survival in all groups was within normal limits for the

strain of animals.

3.3. PUP DATA (F 1 

# GENERATION)

3.3.1. OBSERVATION DATA

(Table 17; Appendix T)

The pups were unremarkable during the lactation period.

3.3.2. BODY WEIGHT DATA

(Tables 16 and 18; Appendix U)

There were no exposure-related differences in body weights or

weight gains in the pups feeding from test substance exposed

animals in the 2000 or 10000 mg/m3 groups, compared to the pups

feeding from Air Control animals. The statistically increased pup

weights at 2000 mg/m3 were related to the statistically significant

decrease in litter size in this group, a common finding unrelated to

exposure. Early in lactation, up to about post-natal day 14, a slight

but statistically significant defic it in pup weight gain occurred at

# Huntingdon Life Sciences 00-4206 Page 39

211-TBA-1G Final Report

20000 mg/m3, such that pup weight at PND 14 was statistically

significantly reduced and was approximately 10% less than the

other groups. Subsequently, weight gain was more comparable

with Control, but at weaning (pos t-natal day 28 in this study) there

remained an approximate 6% deficit in absolute pup weight at

20000 mg/m3 (difference from Control no longer statistically

significant). Other minor inter-group differences in pup body

weight performance were transitory and considered to be not

exposure-related.

3.4. POSTMORTEM DATA

3.4.1. TERMINAL ESTROUS AND MACROSCOPIC

POSTMORTEM EVALUATIONS - PARENTAL

GENERATION

(Table 19; Appendices V, W and AA)

The incidence of estrus or pro-estrus stages among the females in

each group at termination indicated that a normal pattern of

cyclicity was re-established. In 3 of the 4 groups, about onequarter of the females were in one or other of these stages, which

is what would be expected. Only the 2000 mg/m3 group departed

from this pattern, with 10 of the 26 animals in either estrus or proestrus. This was considered to be of no concern and within this

laboratory’s normal range of results.

Compared with controls there was an increased incidence of

discolored foci in the lungs of males administered 10000 and

20000 and in the lungs of females administered 20000 mg/m3.

Most of these foci were tan colored and less than 0.1 cm diameter.

In the absence of any other microscopic finding that correlated

with the tan foci, the increased incidence in this macroscopic

finding is considered potentially related to the test article, but nonadverse. Otherwise, there were no exposure-related differences in

macroscopic postmortem findings in the test substance-exposed

animals compared to the Air Control animals.

# Huntingdon Life Sciences 00-4206 Page 40

211-TBA-1G Final Report

3.4.2. MACROSCOPIC POSTMORTEM EVALUATIONS - PUPS

(Table 20; Appendix X)

There were no exposure-related differences in macroscopic

postmortem evaluations in the pups feeding from test substance

exposed animals compared to th e pups feeding from Air Control

animals including stillborns, pups found dead and pups

cannibalized at the end of th e weaning period. Scattered

observations such as dilated renal pelvis were noted but were not

treatment-related.

3.4.3. ORGAN WEIGHTS - PARENTAL GENERATION

(Table 21; Appendix Y)

Exposure-related effects on organ weights included statistically

significant increases in kidney wei ghts (absolute and/or relative to

body and brain weight) at the 2 higher exposure levels in the

males. These differences for the males were consistent with the

hydrocarbon nephropathy findings at the 20000 mg/m 3 

# exposure

level in the 13-week inhalation study 00-6131 with this same test

substance. However, this finding has been generally accepted (US

EPA 1991. Alpha 2 microglobulin: association with chemically

induced renal toxicity and neoplasia in male rat. In Risk

Assessment Forum, p.85. US Govt. Printing Office, Washington,

DC.) not to be relevant to human risk assessment.

Statistically significant decreases were seen in spleen weights

(absolute and/or relative to body a nd brain weight) at all exposure

levels in the males and/or fe males. Although no effect on the

spleen was noted in the 13-week inhalation study 00-6131

including the histopathological and immunotoxicological

assessments, the differences in this study were considered an

adverse effect since a similar e ffect was seen in the pups (see

below) and considering that th is study involved more exposures

overall (112 vs 65) and more exposures per week (7 vs 5 days)

than the 13-week study. Otherwise, there were no other exposurerelated differences in organ weights in the test substance exposed

animals compared to the Air Control animals. A few other

statistically significant differences in organ weights (absolute

# Huntingdon Life Sciences 00-4206 Page 41

211-TBA-1G Final Report

and/or relative to body and brain weight) were noted for some

exposed groups. However, these were not in a treatment-related

pattern and were in some cases related to decreased body weight

gains earlier discussed.

3.4.4. ORGAN WEIGHTS - PUPS

(Table 22; Appendix Z)

Exposure-related effects on organ weights included statistically

significant decreases in spleen weights (absolute and relative to

brain weight) at the 20000 mg/m 3 

# exposure level. There were no

other exposure-related differences in organ weights in the pups

feeding from test substance exposed animals compared to the pups

feeding from Air Control animals.

3.4.5. HISTOPATHOLOGICAL EVALUATIONS - PARENTAL

GENERATION

(Table 23; Appendix AA)

No test substance related microscopic changes were noted in male

and female reproductive organs or other protocol-specified tissues

in this study. The remaining micr oscopic findings were similar to

those routinely observed in animals of this age and species and

were considered incidental.

3.4.6. SPERM EVALUATIONS – PARENTAL GENERATION

(Appendix BB)

A statistically significant decrease in the caudal epididymis weight

was observed in the high exposure group compared to the control.

This decrease was not considered biologically meaningful because

there was not a similar decrease in the epididymal sperm count.

Group mean values were 0.28 grams for the control group and

0.26 grams for the high-exposure group. Mean percent motility,

epididymal sperm count, homogenization-resistant testicular sperm

count, sperm morphology and caudal epididymis weight were not

affected by treatment at an exposure level of 20000 mg/m 3 

# . No

biologically meaningful differences were observed between the

study groups.

# Huntingdon Life Sciences 00-4206 Page 42

211-TBA-1G Final Report

4. CONCLUSION

Exposure of rats to 2000, 10000 and 20000 mg/m 3 

# of vapor of Gasoline TBA

Vapor Condensate resulted in decreas ed body weight gains in the 20000 mg/m 3



# exposed parental animals during the premating interval and decreased spleen

weights in the 2000, 10000 and 20000 mg/m 3 

# exposed parental animals at their

termination. Increases in kidney weights in parental male animals exposed to the

2 higher exposure levels of vapor were consistent with hydrocarbon nephropathy

seen in similar animals exposed for 13 week s to this test substance. However,

this finding has been generally accepte d (US EPA 1991. Alpha 2 microglobulin:

association with chemically induced renal t oxicity and neoplasia in male rat. In

Risk Assessment Forum, p.85. US Govt. Pr inting Office, Washington, DC.) not to

be relevant to human risk assessment. Therefore, a no observable adverse effect

level (NOAEL) was not determined. There was no effect at any of the exposure

levels on reproductive performance in the study, including mating, fertility,

parturition, lactation and offspring surv ival except a slightly extended mating

period for the 20000 mg/m 3 

# test substance exposed animals and decreases in

spleen weights in pups feeding from 20000 mg/m 3 

# test substance exposed animals

were seen. Therefore, with respect to reproductive performance, a no observable

effect level (NOEL) of 10000 mg/m3 of Gasoline TBA Vapor Condensate was

also concluded with an NOAEL of 20000 mg/m 3



# .

# Huntingdon Life Scien ces 00-4206 Page 43

211- TBA -1G Final Report

REFERENCES

Dunlap, W.P. and Duffy, J.A. 1975. Fortran IV functions for calculating exact

probabilities associated with z, chi-square, t and f values. Behav. Res. Methods and

Instrumentations 7: 59-60.

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV functions for calculating

probabilities associated with Dunnett’s test. Behav. Res. Methods and Instrumentation

13: 363-366.

Kruskal, W.H. 1957. Historical Notes on the Wilcoxon Unpaired Two- Sample Test.

Journal of the American Statistical Association 52: 356-360.

Kruskal, W.H. and Wallis, W.A. 1952. Use of Ranks in One- Criterion Variance Analysis.

Journal of the American Statistical Association 47: 583-621.

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis (1952). Journal of the

American Stati stical Association 48: 907-911.

SAS Institute. 1989. SAS/STAT User’s Guide 1989a. Version 6, Fourth Edition. Cary,

NC: SAS Institute.

Seigel, S. 1956. Nonparametric Statistics for the Behavioral Sciences. McGraw

- Hill.

# Huntingdon Life Sciences 00-4206 Page 44

211- TBA -1G Final Report

CALCULATIONS

Feed Consumption:

grams of feed consumed /kilograms of body weight /day (g/kg/day) =

[(total grams of feed presented - amount of feed remaining) ÷ (average

kilogram body weight)] ÷ no. of days

Female Mating Index:

no. of females with confirmed mating (sperm and/or vaginal plug) plus no. of pregnant

females without evidence of mating (no sperm or vaginal plug)/no. of females placed

with males

Female Fertility Index:

no. of females pregnant/no. of females confirmed mating or pregnancy for females

witho ut evidence of mating

Pregnancy Index:

no. of females pregnant/no. of females inseminated × 100

Male Mating Index:

no. of males with confirmed mating with a female or pregnancy for females without

evidence of mating/no. of males placed with females

M ale Fertility Index:

no. of males mating and impregnating a female plus the no. of males with a pregnant

female without evidence of mating/no. of males with confirmed mating plus no. of males

with a pregnant female without evidence of mating

Gestation In dex:

no. of females with liveborn/no. of females with confirmed pregnancy

Viability Index:

no. of pups alive Day 4 precull/no. of liveborn pups

# Huntingdon Life Sciences 00-4206 Page 45

211- TBA -1G Final Report

CALCULATIONS

Lactation Index:

no. of pups alive Day 21/no. of pups Day 4 postcull

Live Birth Index:

total no. of liveborn pups/total no. of pups born

# Huntingdon Life Sciences 00-4206 Page 46

211-TBA-1G Final Report

General Preface

General Notes

Individual animal data values presented in this report m ay be rounded. Unrounded

individual animal data values are used to calculate the reported m ean and standard

deviation values. Therefore, use of the reported individual values to reproduce m eans,

standard deviations and/or to perform any subsequent calculations m ay produce m inor

discrepancies between the calculated values and those presented in this report.

Key to Abbreviations

M = Male

F = Female

Gen. = General

PG = Pregnant

Group Group

Designation Exposure

Levels

(mg/m 3 

# )

I Control 0 (air only)

II Low 2000

III Middle 10000

IV High 20000

Note:

Dam # 1527 was found dead on Lactation Day 1 and therefore her pups were

subsequently sacrificed. Individual data for this animal and her pups is presented in the

appropriate appendices, but as a sum mary of litter data would be of no value, this data

has not been included in the appropriate summary tables.23

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TABLE 1

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF SURVIVAL AND PREGNANCY

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

No. of males at start N 26 26 26 26

Premating

- Died/sacrificed N 0 0 0 0

Postmating

- Died/sacrificed N 0 0 0 0

No. o f females at start N 26 26 26 26

Died/sacrificed premating N 0 0 0 1

No. of females cohab itated N 26 26 26 25

- Without evidence of mating N 3 3 3 2

Pregnant N 1 0 0 1

Nonpregnant N 2 3 3 1

Pregnant N 21 22 22 22

- Died/sacrificed N 0 0 0 0

- Died delivering N 0 0 0 0

- Died/sacrificed post partum a N 1 1 2 0

- Aborted died/sacrificed N 0 0 0 0

Nonpregnant N 5 4 4 3

- Died/sacrificed N 0 0 0 0

Total females died/sacrificed N 1 1 2 1

% 3.8 3.8 7.7 3.8

Dams delivering N 21 22 22 22

- With liveborn pups N 21 22 22 22

% 100.0 100.0 100.0 100.0

- With all pups stillborn N 0 0 0 0

% 0.0 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significa nt differences

a Includes animals sacrificed following total litter loss.

# Huntingdon Life Sciences 00-4206 Page 48

211-TBA-1G Final Report

Summary of Weekly Clinical Observations Table 2

Total represents a cum ulative total of all an imals with the indicated observation one or

more times during the study.

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m 3



# Group II - 2000 mg/m 3



# Group III - 10000 mg/m 3



# Group IV - 20000 mg/m 3



# Note:

Observations for unmated females were not performed on Study Day 105, due to

technician error.23-JUN-2009 10:42Huntingdon Life Sciences 00-4206 PAGE 49TABLE 2GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS-----------------------------------------------------------------------------------------------------------------------------------DAY OF STUDYGROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119 TOTAL-----------------------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26III 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26NormalWITHIN NORMAL LIMITS I 26 26 26 25 25 25 24 23 23 23 22 21 21 21 20 20 20 21 0 26II 26 26 26 24 25 23 22 22 21 20 20 18 18 19 19 18 18 18 0 26III 26 26 26 24 22 23 20 20 16 16 15 15 16 16 16 15 16 19 0 26IV 26 26 26 26 26 23 23 25 22 19 19 19 19 20 20 20 19 19 0 26DeadTERMINAL SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 26Dermal-GeneralALOPECIA - I 0 0 0 1 1 1 1 1 1 1 2 3 3 3 3 3 3 2 0 3EXTREMITIES/SNOUT II 0 0 0 0 0 0 1 1 3 4 4 4 5 3 3 3 3 3 0 5III 0 0 0 1 3 3 6 6 10 10 10 10 9 9 9 9 9 6 0 10IV 0 0 0 0 0 3 3 1 4 6 6 6 6 5 5 5 6 5 0 9ULCERATION - CERVICAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0II 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0ALOPECIA - GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1II 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 023-JUN-2009 10:42Huntingdon Life Sciences 00-4206 PAGE 50TABLE 2GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS-----------------------------------------------------------------------------------------------------------------------------------DAY OF STUDYGROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119 TOTAL-----------------------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26III 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26BLACK/BROWN STAINS ON I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0SNOUT II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0OcularCHROMODACRYORRHEA - I 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1BILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0CHROMODACRYORRHEA - I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0UNILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Oral/BuccalINCISORS MALOCCLUDED I 0 0 0 0 0 0 1 2 2 2 2 2 2 2 2 2 2 2 0 3II 0 0 0 0 0 2 2 4 3 3 3 4 4 4 4 4 4 4 0 5III 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1IV 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 2 1 0 2INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 2 2 2 0 2II 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 3 3 3 0 4III 0 0 0 0 0 0 0 0 0 1 2 1 1 1 1 1 1 1 0 2IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 123-JUN-2009 10:42Huntingdon Life Sciences 00-4206 PAGE 51TABLE 2GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS-----------------------------------------------------------------------------------------------------------------------------------DAY OF STUDYGROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 119 TOTAL-----------------------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26III 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26ORAL SORE I 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 023-JUN-2009 10:43Huntingdon Life Sciences 00-4206 PAGE 52TABLE 2GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSFEMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS-----------------------------------------------------------------------------------------------------------------------------------DAY OF STUDYGROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL-----------------------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 21 3 3 3 3 0 2II 26 26 26 26 26 26 26 26 26 26 26 23 4 3 3 3 0 3III 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 3 0 3IV 26 26 26 26 26 26 26 26 25 25 25 21 3 2 2 1 0 1NormalWITHIN NORMAL LIMITS I 26 26 26 26 26 26 26 25 25 25 26 21 3 3 3 3 0 0 26II 26 26 26 26 26 26 25 24 24 24 24 21 4 3 3 3 0 0 26III 26 26 26 25 25 25 23 25 25 24 23 21 3 2 2 2 0 0 26IV 26 26 26 26 26 26 25 24 20 20 22 18 3 2 2 1 0 0 26DeadFOUND DEAD I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0IV 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1TERMINAL SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1Dermal-GeneralALOPECIA - I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0EXTREMITIES/SNOUT II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 1 1 1 2 0 0 1 1 1 0 0 0 0 0 0 2IV 0 0 0 0 0 0 0 0 2 2 2 2 0 0 0 0 0 0 2ALOPECIA - GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 023-JUN-2009 10:43Huntingdon Life Sciences 00-4206 PAGE 53TABLE 2GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSFEMALES SUMMARY OF WEEKLY CLINICAL OBSERVATIONS-----------------------------------------------------------------------------------------------------------------------------------DAY OF STUDYGROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL-----------------------------------------------------------------------------------------------------------------------------------# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 21 3 3 3 3 0 2II 26 26 26 26 26 26 26 26 26 26 26 23 4 3 3 3 0 3III 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 3 0 3IV 26 26 26 26 26 26 26 26 25 25 25 21 3 2 2 1 0 1YELLOW ANO-GENITAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0STAINING II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0IV 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 2OcularCHROMODACRYORRHEA - I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0UNILATERAL II 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1III 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1IV 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1Oral/BuccalINCISORS MALOCCLUDED I 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1II 0 0 0 0 0 0 1 2 2 2 2 2 0 0 0 0 0 0 2III 0 0 0 0 0 0 1 1 1 1 1 1 0 0 0 0 0 0 1IV 0 0 0 0 0 0 1 1 1 1 2 2 0 0 0 0 0 0 2INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0IV 0 0 0 0 0 0 1 1 1 1 2 2 0 0 0 0 0 0 223

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TABLE 3

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF CLINICAL OBSERVATIONS DURING GESTATION - (frequency/animals)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 to 25

Normal

----------

WITHIN NORMAL LIMITS 89/23 79/21 80/21 86/22

TERMINAL SACRIFICE 2/ 2 1/ 1 1/ 1 2/ 2

Dermal

- General

------------------

ALOPECIA - EXTREMI TIES/SNOUT 0/ 0 2/ 1 4/ 1 3/ 2

Ocular

----------

CHROMODACRYORRHEA - UNILATERAL 0/ 0 5/ 2 1/ 1 0/ 0

Oral/Buccal

---------------

INCISORS MALOCCLUDED 3/ 1 7/ 3 5/ 2 4/ 1

INCISORS BROKEN/MISSING 0/ 0 4/ 1 3/ 3 4/ 123

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TABLE 4

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MATERNAL CLINICAL OBSERVATIONS DURING LACTATION - (frequency/animals)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 1 to 28

Normal

----------

WITHIN NORMAL LIMITS 106/18 107/18 107/18 122/21

FOUND DEAD 1/ 1 0/ 0 0/ 0 0/ 0

TERMINAL SACRIFICE 20/20 22/22 22/22 22/22

Gen. Appearance

-------------------

EARS SWOLLEN/RED

- BILATERAL 0/ 0 0/ 0 6/ 1 0/ 0

Dermal

- General

------------------

ALOPECIA - EXTREMITIES/SNOUT 0/ 0 0/ 0 4/ 1 0/ 0

Ocular

----------

CHROMODACRYORRHEA - UNILATERAL 1/ 1 8/ 2 6/ 1 0/ 0

Oral/Buccal

---------------

INCISORS MALOCCLUDED 13/ 3 18/ 3 12/ 2 6/ 1

INCISOR S BROKEN/MISSING 6/ 1 17/ 3 4/ 1 10/ 223

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TABLE 5

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY

- 7 MEAN 116 116 116 116

S.D. 9.1 9.1 9.2 9.5

N 26 26 26 26

DAY 0 MEAN 185 183 182 181

S.D. 11.2 12.6 11.9 9.8

N 26 26 26 26

DAY 7 MEAN 241 240 239 232

S.D. 15.2 15.1 15.5 10.4

N 26 26 26 26

DAY 14 MEAN 299 298 294 284*

S.D. 18.8 18.8 20.5 12.6

N 26 26 26 26

DAY 21 MEAN 348 344 338 327**

S.D. 24.4 21.8 24 .8 15.8

N 26 26 26 26

DAY 28 MEAN 377 377 369 358*

S.D. 28.5 25.7 25.8 17.8

N 26 26 26 26

D AY 35 MEAN 409 410 398 385**

S.D. 33.2 27.8 28.8 20.1

N 26 26 26 26

DAY 42 MEAN 432 435 419 413

S.D. 36.6 30.4 31.0 23.3

N 26 26 26 26

DAY 49 MEAN 455 457 441 430*

S.D. 39.8 31.7 34.3 25.9

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

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TABLE 5

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALAT ION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 56 MEAN 468 472 458 444*

S.D. 41.4 33.6 35.9 26.6

N 26 26 26 26

DAY 63 MEAN 484 491 475 460*

S.D. 43.1 34.1 39.9 29.2

N 26 26 26 26

DAY 69 MEAN 498 503 487 468*

S.D. 46.2 35.6 42.1 31.2

N 26 26 26 26

DAY 77 MEAN 510 514 498 479*

S.D. 47.1 35.4 42.2 29.5

N 26 26 26 26

DAY 84 MEAN 523 528 511 488*

S.D. 50.1 35.7 47.1 33.3

N 26 26 26 26

DAY 91 MEAN 534 539 521 497**

S.D. 48.2 37.6 47.6 35.3

N 26 26 26 26

DAY 98 MEAN 546 550 528 504**

S.D. 51.6 38.1 49.4 35.8

N 26 26 26 26

DAY 105 MEAN 555 563 540 518*

S.D. 52.2 39.7 49.7 35.9

N 26 26 26 26

DAY 112 MEAN 567 573 553 531*

S.D. 56.8 41.1 51.6 35.7

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Stati stical key: * = p<0.05 ** = p<0.0123

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- 2009 10:49

Huntingdon Life Sciences 00

- 4206 PAGE 58

TABLE 5

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

----------------------------------------------------------------------------------------------------------------------------- - -----

DAY

- 7 MEAN 132 132 131 131

S.D. 9.0 9.0 9.1 8.9

N 26 26 26 26

DAY 0 MEAN 169 167 166 165

S.D. 10.9 10.9 10.1 9.8

N 26 26 26 26

DAY 7 MEAN 194 193 194 185

S.D. 13.7 13.4 13.8 12.2

N 26 26 26 26

DAY 14 MEAN 218 215 213 206*

S.D. 15.1 16.8 16.3 12.6

N 26 26 26 26

DAY 21 MEAN 237 234 232 223**

S.D. 16.5 17.7 16.9 13.2

N 26 26 26 26

DAY 28 MEAN 248 246 244 236

S.D. 17.7 18.8 18.1 15.5

N 26 26 26 26

DAY 35 MEAN 261 260 254 247*

S.D. 19.6 21.2 18.6 16.4

N 26 26 26 26

DAY 42 MEAN 268 270 263 259

S.D. 21.5 21.0 18.8 15.9

N 26 26 26 25

DAY 49 MEAN 280 278 270 264*

S.D. 22.6 23.6 17.5 15.7

N 26 26 26 25

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 10:49

Huntingdon Life Sciences 00

- 4206 PAGE 59

TABLE 5

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT VALUES (GR AMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 56 MEAN 284 284 277 269*

S.D. 23.7 23.1 19.1 16.8

N 26 26 26 25

DAY 63 MEAN 290 291 286 273*

S.D. 25.0 24.1 19.8 16.8

N 26 26 26 25

DAY 69 MEAN 291 293 289 275*

S.D. 24.6 25.3 20.6 18.1

N 26 26 26 25

DAY 77 MEAN 311 298 322 304

S.D. 32.1 23.9 22.9 3.9

N 3 4 3 3

DAY 84 MEAN 324 300 338 318

S.D. 49.8 13.5 29.7 15.3

N 3 3 3 2

DAY 91 MEAN 328 297 322 343

S.D. 6 4.9 5.7 25.5 49.9

N 3 3 3 2

DAY 98 MEAN 364 318 325 311

S.D. 114.4 20.2 24.5 0.0

N 3 4 5 1

DAY 105 MEAN 310 324 330 325

S.D. 34.6 16.6 27.4 0.0

N 2 4 5 1

DAY 112 MEAN 354 329

S.D. 0.0 15.7

N 1 2

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 10:49

Huntingdon Life Sciences 00

- 4206 PAGE 60

TABLE 5

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 113 MEAN 351 331

S.D. 0.0 24.0

N 1 2

DAY 114 MEAN 342 331

S.D. 0.0 11.6

N 1 2

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant diffe rences23

- JUN

- 2009 10:51

Huntingdon Life Sciences 00

- 4206 PAGE 61

TABLE 6

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY

- 7 TO 0 MEAN 69 67 66 65*

S.D. 4.9 4.6 5.1 3.3

N 26 26 26 26

DAY 0 TO 7 MEAN 56 56 57 51**

S.D. 6.3 4.5 6.2 5.4

N 26 26 26 26

DAY 7 TO 14 MEAN 58 58 55 52**

S.D. 7.8 6.0 8.1 5.7

N 26 26 26 26

DAY 14 TO 21 MEAN 49 46 45 43**

S.D. 7.9 6.5 7.3 6.5

N 26 26 26 26

DAY 21 TO 28 MEAN 29 33 30 31

S.D. 6.8 6.9 7.2 4.7

N 26 26 26 26

DAY 28 TO 35 MEAN 32 33 30 27*

S.D. 7.4 6.0 6.3 6.4

N 26 26 26 26

DA Y 35 TO 42 MEAN 23 24 21 28*

S.D. 5.3 6.9 6.0 9.9

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p< 0.05 ** = p<0.0123

- JUN

- 2009 10:51

Huntingdon Life Sciences 00

- 4206 PAGE 62

TABLE 6

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEV EL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 42 T O 49 MEAN 23 22 22 17**

S.D. 5.9 5.1 6.2 9.4

N 26 26 26 26

DAY 49 TO 56 MEAN 13 16 17 14

S.D. 6.4 5.0 5.7 6.3

N 26 26 26 26

DAY 56 TO 63 MEAN 16 18 17 16

S.D. 5.6 4.8 6.9 5.3

N 26 26 26 26

DAY 63 TO 69 MEAN 13 12 12 9*

S.D. 7.2 6.3 5.6 6.1

N 26 26 26 26

DAY 69 TO 77 MEAN 12 11 10 11

S.D. 7.2 7.3 11.8 8.2

N 26 26 26 26

DAY 77 TO 84 MEAN 13 14 13 9

S.D. 5.3 7.4 13.2 7.0

N 26 26 26 26

DAY 84 TO 91 MEAN 11 11 10 9

S.D. 6.2 4.5 5.3 7.1

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 10:51

Huntingdon Lif e Sciences 00

- 4206 PAGE 63

TABLE 6

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES ME AN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 91 TO 98 MEAN 13 11 7* 7*

S.D. 8.3 5.0 5.9 9.2

N 26 26 26 26

DAY 98 TO 105 MEAN 9 13* 12 14*

S.D. 5.9 7.8 4.9 7.2

N 26 26 26 26

DAY 105 TO 112 MEAN 12 9 13 13

S.D. 6.6 6.3 6.2 4.6

N 26 26 26 26

DAY 0 TO 69 MEAN 313 319 306 287*

S.D. 41.1 29.9 36.9 30.7

N 26 26 26 26

DAY 0 TO 112 MEAN 382 389 371 350*

S.D. 51.3 36.5 47.9 35.5

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key : * = p<0.0523

- JUN

- 2009 10:53

Huntingdon Life Sciences 00

- 4206 PAGE 64

TABLE 6

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DA Y

- 7 TO 0 MEAN 37 35 35 34

S.D. 5.3 5.6 5.8 5.7

N 26 26 26 26

DAY 0 TO 7 MEAN 25 26 28 20*

S.D. 5.8 6.6 6.5 7.4

N 26 26 26 26

DAY 7 TO 14 MEAN 24 22 19 21

S.D. 6.1 6.9 6.3 7.2

N 26 26 26 26

DAY 14 TO 21 MEAN 19 19 19 17

S.D. 5.8 5.6 6.4 7.1

N 26 26 26 26

DAY 21 TO 28 MEAN 11 12 12 14

S.D. 6.2 5.9 6.9 7.7

N 26 26 26 26

DAY 28 TO 35 MEAN 13 14 10 11

S.D. 5.3 6.5 6.3 8.3

N 26 26 26 26

DAY 35 TO 42 MEAN 7 10 9 11

S.D. 6.8 4.8 6.2 8.5

N 26 26 26 25

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 10:53

Huntin gdon Life Sciences 00

- 4206 PAGE 65

TABLE 6

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 42 TO 49 MEAN 11 9 8 5**

S.D. 6.4 6.3 7.4 7.4

N 26 26 26 25

DAY 49 TO 56 MEAN 5 6 7 5

S.D. 5.4 7.2 7.2 6.2

N 26 26 26 25

DAY 56 TO 63 MEAN 5 6 9 4

S.D. 8.1 10.2 8.1 7.4

N 26 26 26 25

DAY 63 TO 69 MEAN 2 2 3 3

S.D. 8.5 8.5 11.1 8.5

N 26 26 26 25

DAY 69 TO 77 MEAN 18 6 9 16

S.D. 13.0 8.3 19.5 12.1

N 3 4 3 3

DAY 77 TO 84 MEAN 13 14 16 13

S.D. 23.3 13.4 10.9 12.4

N 3 3 3 2

DAY 84 TO 91 MEAN 4

- 3

- 16 25

S.D. 23.9 8.4 7.1 34.6

N 3 3 3 2

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0123

- JUN

- 2009 10:53

Huntingdon Life Sciences 00

- 4206 PAGE 66

TABLE 6

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 91 TO 98 MEAN 36 10 4 3

S.D. 53.5 4.1 11.4 0.0

N 3 3 3 1

DAY 98 TO 105 MEAN 11 6 5 14

S.D. 8.1 3.7 5.1 0.0

N 2 4 5 1

DAY 0 TO 69 MEAN 122 126 123 110*

S.D. 17.2 20.1 15.6 13.6

N 26 26 26 25

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 10:57

Huntingdon Life Sciences 00

- 4206 PAGE 67

TABLE 7

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF GESTATION BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 MEAN 294 291 283 275*

S.D. 26.4 26.5 19.5 15.8

N 20 22 22 21

DAY 4 MEAN 317 317 309 301

S.D. 27.9 29.2 19.7 19.5

N 20 22 22 21

DAY 7 MEAN 327 328 319 310

S.D. 30.6 29.2 20.4 19.1

N 20 22 22 21

DAY 14 MEAN 357 355 347 341

S.D. 30.8 30.9 21.3 21.2

N 20 22 22 21

DAY 20 MEAN 418 410 407 403

S.D. 33.0 33.6 31.5 28.0

N 20 22 22 21

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 10:57

Huntingdon Life Sciences 00

- 4206 PAGE 68

TABLE 8

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF GESTATION BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 0 TO 4 MEAN 23 26 27 25

S.D. 5.9 6.6 6.5 7.4

N 20 22 22 21

DAYS 4 TO 7 MEAN 10 11 10 10

S.D. 5.0 3.1 3.6 3.8

N 20 22 22 21

DAYS 7 TO 14 MEAN 30 27 28 31

S.D. 4.6 5.9 8.1 5.5

N 20 22 22 21

DAYS 14 TO 20 MEAN 61 55 60 62

S.D. 12.9 16.6 24.1 13.2

N 20 22 22 21

DAYS 0 TO 20 MEAN 124 119 125 128

S.D. 11.5 19.7 28.4 16.9

N 20 22 22 21

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 10:59

Huntingdon Life Sciences 00

- 4206 PAGE 69

TABLE 9

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MATERNAL LACTATION BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 1 MEAN 324 319 318 307

S.D. 26.3 29.0 21.7 18.2

N 19 22 21 22

DAY 4 MEAN 332 327 323 321

S.D. 29.1 28.3 21.6 23.4

N 20 21 20 22

DAY 7 MEAN 350 334 341 333

S.D. 31.1 30.3 21.2 21.7

N 20 21 20 22

DAY 14 MEAN 370 355 365 350*

S.D. 23.9 26.0 21.3 24.3

N 20 21 20 22

DAY 21 MEAN 355 351 350 342

S.D. 26.2 23.7 16.1 23.2

N 20 21 20 22

DAY 28 MEAN 330 329 329 325

S.D. 23.4 25.6 18.6 23.2

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 11:00

Huntingdon Life Sciences 00

- 4206 PAGE 70

TABLE 10

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 1 TO 4 MEAN 7 9 7 14

S.D. 11.9 10.4 14.1 12.4

N 19 21 20 22

DAYS 4 TO 7 MEAN 18 7 18 12

S.D. 15.3 15.9 13.7 12.9

N 20 21 20 22

DAYS 7 TO 14 MEAN 20 21 24 17

S.D. 9.7 20.0 11.1 15.4

N 20 21 20 22

DAYS 14 TO 21 MEAN

- 15

- 4*

- 15

- 8

S.D. 12.3 9.7 14.8 16.0

N 20 21 20 22

DAYS 21 TO 28 MEAN

- 25

- 21

- 21

- 17

S.D. 14.3 15.8 12.2 16.5

N 20 21 20 22

DAYS 1 TO 28 MEAN 5 11 13 19**

S.D. 14.6 11.7 13.8 16.2

N 19 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:01

Huntingdon Life Sciences 00

- 4206 PAGE 71

TABLE 11

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 MEAN 153 155 149 148

S.D. 8.1 8.7 6.9 7.5

N 26 26 26 25

DAY 7 MEAN 117 115 116 114

S.D. 5.1 5.1 4.5 5.7

N 26 26 26 26

DAY 14 MEAN 103 100* 99** 98**

S.D. 4.8 5.3 3.6 4.6

N 26 26 26 26

DAY 21 MEAN 92 89* 88** 91

S.D. 4.4 4.0 3.9 5.2

N 26 26 26 26

DAY 28 MEAN 79 78 79 80

S.D. 4.1 3.7 5.0 4.3

N 26 26 26 26

DAY 35 MEAN 74 72 71 73

S.D. 4.1 2.7 3.6 4.5

N 26 26 26 26

DA Y 42 MEAN 68 66 66 68

S.D. 4.3 2.7 2.5 4.1

N 26 26 26 26

DAY 49 MEAN 64 63 63 63

S.D. 3.4 2.7 3.0 3.2

N 26 26 26 26

DAY 56 MEAN 62 60* 60 62

S.D. 3.5 2.6 2.3 2.9

N 26 26 26 26

DAY 63 MEAN 60 58 60 60

S.D. 3.8 2.7 2.0 2.8

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:01

Huntingdon Life Sciences 00

- 4206 PAGE 72

TABLE 11

GASOLINE T BA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 69 MEAN 56 55 54 53

S.D. 3.9 3.0 2.2 10.0

N 26 26 26 26

DAY 91 MEAN 51 49 51 52

S.D. 3.6 2.6 2.3 3.3

N 26 26 26 26

DAY 98 MEAN 50 48 48 51

S.D. 3.6 2.4 2.2 4.0

N 26 26 26 26

DAY 105 MEAN 48 48 47 50

S. D. 4.2 2.6 2.3 3.3

N 26 26 26 26

DAY 112 MEAN 47 45 47 50*

S.D. 2.9 3.0 2.7 3.4

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 11:03

Huntingdon Life Sciences 00

- 4206 PAGE 73

TABLE 11

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATI ON REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 MEAN 127 130 130 133

S.D. 8.3 6.2 9.7 9.2

N 25 26 25 26

DAY 7 MEAN 98 101 100 97

S.D. 6.1 4.8 7.0 7.1

N 26 26 26 25

DAY 14 MEAN 94 95 94 93

S.D. 4.5 6.5 6.7 4.8

N 26 26 26 26

DAY 21 MEAN 88 91 89 89

S.D. 4.9 5.6 5.4 4.9

N 26 26 26 26

DAY 28 MEAN 80 85** 83 84

S.D. 4.6 5.4 7.2 4.0

N 26 26 26 26

DAY 35 MEAN 78 81* 79 81*

S.D. 5.0 5.4 4.7 4.8

N 26 26 26 26

DAY 42 MEAN 71 76** 75* 78**

S.D. 4.4 5.4 5.7 4.6

N 26 26 26 25

DAY 49 MEAN 71 72 72 72

S.D. 3.5 5.4 4.8 4.4

N 26 26 26 25

DAY 56 MEAN 67 68 67 68

S.D. 3.3 3.8 4.4 2.8

N 26 26 26 25

DAY 63 MEAN 66 69 70 68

S.D. 4.4 7.5 5.9 4.7

N 26 26 26 25

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:03

Hu ntingdon Life Sciences 00

- 4206 PAGE 74

TABLE 1 1

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 69 MEAN 63 65 62 65

S.D. 3.6 7.1 9.8 4.6

N 25 25 26 25

DAY 91 MEAN 59 59 56 60

S.D. 11.2 3.5 0.9 0.4

N 3 3 3 2

DAY 98 MEAN 57 56 51 53

S.D. 2.0 6.7 4.5 0.0

N 3 3 3 1

DAY 105 MEAN 57 54 53 58

S.D. 2.6 5.7 4.4 0.0

N 2 4 5 1

DAY 112 MEAN 49 51

S.D. 0.0 7.8

N 1 2

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 11:04

Huntingdon Life Sciences 00

- 4206 PAGE 75

TABLE 12

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF GESTATION FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 0 TO 4 MEAN 74 74 74 74

S.D. 6.3 7.6 5.3 6.5

N 20 22 21 21

DAYS 4 TO 7 MEAN 74 76 76 76

S.D. 4.3 10.0 6.3 6.2

N 20 22 22 21

DAYS 7 TO 14 MEAN 70 71 71 71

S.D. 3.9 6.0 4.2 9.6

N 20 22 22 21

DAYS 14 TO 20 MEAN 62 62 61 65

S.D. 5.7 5.7 11.1 3.7

N 20 22 22 21

DAYS 0 TO 20 MEAN 70 71 70 72

S.D. 3.4 6.5 4.9 5.1

N 20 22 22 21

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 11:05

Huntingdon Life Sciences 00

- 4206 PAGE 76

TABLE 13

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF LACTATION FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 1 TO 4 MEAN 106 92* 102 100

S.D. 10.3 17.6 17.9 14.0

N 19 21 20 22

DAYS 4 TO 7 MEAN 115 108 113 109

S.D. 15.3 16.3 14.1 11.4

N 20 21 20 22

DAYS 7 TO 14 MEAN 144 128** 137 136

S.D. 7.7 16.8 13.0 6.8

N 20 20 20 22

DAYS 14 TO 21 MEAN 172 174 165 171

S.D. 14.7 23.3 18.2 12.0

N 20 21 20 22

DAYS 21 TO 28 MEAN 307 299 301 291

S.D. 37.0 45.1 26.1 29.0

N 20 21 20 22

DAYS 1 TO 28 MEAN 169 160 164 162

S.D. 12.8 19.5 13.5 9.0

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0108

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 77

TABLE 1 4

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ESTROUS STAGES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

PREMATING TREATMENT PERIOD (21 days evaluated) a

F EMALES EXAMINED N 26 26 26 26

CYCLE LENGTH (days) MEAN 4.3 4.2 4. 3 4.1

S.D. 0.56 0.43 0. 80 0.49

N 26 2 4 23 25

NUMBER OF E S TROUS CYCLES MEAN 3.8 3.4 3. 2 3.8

S.D. 0.99 0.97 1. 24 0.85

N 26 2 4 23 25

-----------------------------------------------------------------------------------------------------------------------------------

a A ny periods of pseudopregnancy were excluded from these calculations (see also Appendix P).

No statistically significant differences23

- JUN

- 2009 11:07

Huntingdon Life Sciences 00

- 4206 PAGE 78

TABLE 15

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF COHABITATION DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Males placed with females N 26 26 26 25

T otal number mated N 24 23 23 24

male mating index % 92.3 88.5 88.5 96.0

with females pre gnant N 21 22 22 22

male fertility index % 80.8 84.6 84.6 88.0

Females with defined

day 0 of Gestation N 23 23 23 23

No. of days until Mating MEAN 2.7 3.0 3.0 4.0

S.D. 1.60 1.69 1.77 2.70

Day 1 to 4 N 21 21 18 14

% 91.3 91.3 78.3 60.9

Day 5 to 8 N 2 1 5 8

% 8.7 4.3 21.7 34.8

Day 9 to 14 N 0 1 0 1

% 0.0 4.3 0.0 4.3

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 11:10

Huntingdon Life Sciences 00

- 4206 PAGE 79

TABLE 16

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Females on Study N 26 26 26 26

Fem ales Mated N 24 23 23 24

Mating Index % 92.3 88.5 88.5 92.3

Females Pregnant N 21 22 22 22

Female Fertility Index % 87.5 95.7 95.7 91.7

Females with Liveborn N 21 22 22 22

Gestation Index % 100.0 100.0 100.0 100.0

Females Completing Delivery N 21 22 22 22

% 100.0 100.0 100.0 100.0

with Stillborn Pups N 1 5 5 0

% 4.8 22.7 22.7 0.0

with all Stillborn N 0 0 0 0

% 0.0 0.0 0.0 0.0

Litters with Liveborn,

but no Pups Alive

day 4 N 0 1 1 0

% 0.0 4.5 4.5 0.0

day 21 N 0 1 1 0

% 0.0 4.5 4.5 0.0

Duration of Gestation MEAN 22.0 22 .2 22.0 22.0

S.D. 0.00 0.39 0.58 0.45

N 20 22 22 21

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 11:10

Huntingdon Life Sciences 00

- 4206 PAGE 80

TABLE 16

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters with Liveborn Pups N 21 22 22 22

Pups Delivered (total) N 300 264 303 310

MEAN 14.3 12.0* 13.8 14.1

S.D. 1.23 3.83 2.45 2.09

Liveborn N 299 259 291** 310

Live Birth Index % 99.7 98.1 96.0 100.0

Stillborn N 1 5 12** 0

% 0.3 1.9 4.0 0.0

Culled day 4 84 54 76 78

Liveborn, not culled N 215 205 215 232

prior to day 21

Pups Dying, Missing, and/or Cannibalized

day 0 N 0 0 0 0

% 0.0 0.0 0.0 0.0

days 1

- 4 N 1 4 18** 12**

% 0.3 1.5 6.2 3.9

days 5

- 21 N 1 2 0 1

% 0.3 0.8 0.0 0.3

days 0

- 4 N 1 4 18** 12**

% 0.3 1.5 6.2 3.9

days 0

- 21 N 2 6 18** 13*

% 0.7 2.3 6.2 4.2

Pups Surviving 4 days N 286 255 273 298

Viability Index % 95. 7 98.5 93.8 96.1

Pups Surviving 21 days N 201 199 197 219

Lactation Index % 99.5 99.0 100.0 99.5

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:10

Huntingdon Life Sciences 00

- 4206 PAGE 81

TABLE 16

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION R EPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Implantation Sites N 318 296 336 336

per Litter MEAN 15.1 14.1 15.3 15.3

S.D. 1.53 2.43 1.32 1.16

Sex Ratio

- Male Pups:Total Pups

day 0 N 153 118 143 159

% 51.2 45.6 49.1 51.3

day 21 N 104 93 100 113

% 51.7 46.7 50.8 51.6

Live Pups/Litter

day 1 MEAN 14.2 11.7* 13.2 13.8

S.D. 1.22 3.8 5 3.51 2.16

N 21 22 21 22

day 4 preculling MEAN 13.6 12.1 13.0 13.5

S.D. 2.97 3.05 3.55 2.04

N 21 21 21 22

day 4 postculling MEAN 9.6 9.6 9.4 10.0

S.D. 1.75 1.66 1.91 0.31

N 21 21 21 22

day 7 MEAN 9.6 9.6 9.4 10.0

S.D. 1.75 1.66 1.91 0.38

N 21 21 21 22

day 14 MEAN 9.6 9.5 9.4 10.0

S.D. 1.75 1.63 1.91 0.38

N 21 21 21 22

day 21 MEAN 9.6 9.5 9.4 10.0

S.D. 1.75 1.63 1.91 0.38

N 21 21 21 22

day 28 MEAN 9.6 9.5 9.4 10.0

S.D. 1.75 1.63 1.91 0.38

N 21 21 21 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 11:10

Huntingdon Life Sciences 00

- 4206 PAGE 82

TABLE 16

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STU DY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

----------------------------------------------------------------------------------------------------------------------------------- Pup Weight/Litter (grams)

day 1 MEAN 6.9 7.5* 6.9 7.0

S.D. 0.56 0.73 0.71 0.64

N 21 22 20 22

day 4 MEAN 9.9 11.0* 10.0 9.9

S.D. 1.15 1.39 1.58 1.02

N 20 21 20 22

day 7 MEAN 13.9 14.7 13.9 13.1

S.D. 1.85 1.44 2.14 1.30

N 20 21 20 22

day 14 MEAN 25.1 24.6 24.3 22.7*

S.D. 3.17 2.64 2.86 2.09

N 20 21 20 22

day 21 MEAN 40.2 41.3 40.6 37.9

S. D. 5.32 4.40 6.03 3.85

N 20 21 20 22

day 28 MEAN 76.3 77.6 76.5 71.9

S.D. 8.39 6.14 9.45 5.30

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 11:13

Huntingdon Life Sciences 00

- 4206 PAGE 83

TABLE 17

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP CLINICAL OBSERVATIONS DURING LACTATION - (frequency/animals)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 1 to 28

Normal

----------

WITHIN NORMAL LIMITS 1081/20 1 038/21 1046/20 1169/2223

- JUN

- 2009 11:16

Huntingdon Life Sciences 00

- 4206 PAGE 84

TABLE 18

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 1 males MEAN 7.2 7.8* 7.1 7.2

S.D. 0.52 0.74 0.68 0.69

N 20 21 20 22

1 females MEAN 6.7 7.3** 6.6 6.7

S.D. 0.52 0.72 0.50 0.64

N 20 22 19 22

1 males+females MEAN 7.0 7.5* 6.9 7.0

S.D. 0.50 0.73 0.71 0.64

N 20 22 20 22

day 4 males MEAN 10.2 11.3* 10.3 10.1

preculling S.D. 1.16 1.38 1.57 1.09

N 20 21 20 22

4 females MEAN 9.6 10.8** 9.5 9.6

preculling S.D. 1.19 1.44 0.77 1.03

N 20 21 19 22

4 males+females MEAN 9.9 11.0* 10.0 9.9

preculling S.D. 1.15 1.39 1.58 1.02

N 20 21 20 22

da y 4 males MEAN 10.2 11.3* 10.3 10.1

postculling S.D. 1.20 1.41 1.57 1.12

N 20 21 20 22

4 females MEAN 9.6 10.8** 9.5 9.6

postculling S.D. 1.21 1.44 0.75 1.02

N 20 21 19 22

4 males+females MEAN 9.9 11.0* 10.0 9.9

postculling S.D. 1.18 1.40 1.58 1.04

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:16

Huntingdon Life Sciences 00

- 4206 PAGE 85

TABLE 18

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 7 males MEAN 14.3 15.0 14.2 13.5

S.D. 2.03 1.39 2.12 1.38

N 20 21 20 22

7 females MEAN 13.5 14.5 13.2 12.8

S.D. 1.74 1.61 1.24 1.32

N 20 21 19 22

7 males+females MEAN 13.9 14.7 13.9 13.1

S.D. 1.85 1.44 2.14 1.30

N 20 21 20 22

day 14 males MEAN 25.5 25.0 24.7 23.1*

S.D. 3.39 2.65 2.84 2.12

N 20 21 20 22

14 females MEAN 24.6 24.2 23.4 22.3*

S. D. 3.03 2.74 1.88 2.17

N 20 21 19 22

14 males+females MEAN 25.1 24.6 24.3 22.7*

S.D. 3.17 2.64 2.86 2.09

N 20 21 20 22

day 21 males MEAN 40.8 41.9 41.1 38.2

S.D. 6.09 4.52 6.12 3.86

N 20 21 20 22

21 females MEAN 39.6 40.7 39 .2 37.5

S.D. 4.75 4.46 4.37 4.03

N 20 21 19 22

21 males+females MEAN 40.2 41.3 40.6 37.9

S.D. 5.32 4.40 6.03 3.85

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistic al key: * = p<0.0523

- JUN

- 2009 11:16

Huntingdon Life Sciences 00

- 4206 PAGE 86

TABLE 18

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 28 males MEAN 79.3 81.0 79.1 73.9

S.D. 10.13 6.83 9.35 5.45

N 20 21 20 22

28 females MEAN 73.0 74.5 72.1 69.7

S.D. 7.04 5.79 6.27 5.56

N 20 21 19 22

28 males+females MEAN 76.3 77.6 76.5 71.9

S.D. 8.39 6.14 9.45 5.30

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 11:16

Huntingdon Life Sciences 00

- 4206 PAGE 87

TABLE 18

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHAL ATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP BODY WEIGHT GAIN

-- GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 1 - 4 males MEAN 3.0 3.5 3.2 2.9

S.D. 0.77 0.82 0.97 0.66

N 20 21 20 22

females MEAN 2.9 3.4 2.9 2.9

S.D. 0.80 0.89 0.40 0.63

N 20 21 19 22

males+females MEAN 3.0 3.4 3.1 2.9

S.D. 0.77 0.85 0.96 0.62

N 20 21 20 22

day 4 - 7 males MEAN 4.1 3.8 4.0 3.4*

S.D. 0.97 0.77 0.78 0.65

N 20 21 20 22

females MEAN 3.9 3.7 3.7 3.2**

S.D. 0.77 0.78 0.69 0.69

N 20 21 19 22

males+females MEAN 4.0 3.7 3.9 3.3**

S.D. 0.85 0.74 0.77 0.66

N 20 21 20 22

day 4

- 21 males MEAN 30.6 30.6 30.8 28.1

S.D. 5.24 4. 04 4.86 3.57

N 20 21 20 22

females MEAN 30.0 29.9 29.7 27.9

S.D. 4.11 3.75 3.82 3.68

N 20 21 19 22

males+females MEAN 30.3 30.3 30.6 28.0

S.D. 4.59 3.80 4.71 3.54

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Sta tistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:16

Huntingdon Life Sciences 00

- 4206 PAGE 88

TABLE 18

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP BODY WEIGHT GAIN

-- GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

----------------------------------------------------------------------------------------------------------------------------- - -----

day 4

- 28 males MEAN 69.2 69.8 68.9 63.8

S.D. 9.25 6.43 8.10 5.11

N 20 21 20 22

females MEAN 63.4 63.7 62.6 60.1

S.D. 6.29 5.18 5.80 5.11

N 20 21 19 22

males+females MEAN 66.4 66.6 66.4 62.1

S.D. 7.61 5.64 8.13 4.93

N 20 21 20 22

day 7

- 14 males MEAN 11.2 10.0 10.4 9.6**

S.D. 1.74 2.10 1.09 1.47

N 20 21 20 22

females MEAN 11.1 9.7* 10.3 9.5**

S.D. 1.91 1.70 0.95 1.47

N 20 21 19 22

males+females MEAN 11.2 9.9* 10.4 9.6**

S.D. 1.81 1.84 1.01 1.45

N 20 21 20 22

day 7

- 28 males MEAN 65.1 66.0 64.9 60.4

S.D. 8.37 6.02 7.62 4.76

N 20 21 20 22

females MEAN 59.5 60.0 58.9 56.9

S.D. 5.63 4.62 5.43 4.67

N 20 21 19 22

males+females MEAN 62.4 62.9 62.6 58.8

S.D. 6.84 5.18 7.62 4.54

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0123

- JUN

- 2009 11:16

Huntingdon Life Sciences 00

- 4206 PAGE 89

TABLE 18

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP BODY WEIGHT GAIN

-- GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I I I III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 14

- 21 males MEAN 15.3 16.9 16.4 15.1

S.D. 3.00 2.33 3.49 2.06

N 20 21 20 22

females MEAN 15.0 16.5 15.8 15.2

S.D. 2.20 2.05 2.61 2.02

N 20 21 19 22

males+females MEAN 15.2 16.7 16.3 15.2

S.D. 2.49 2.09 3.31 1.96

N 20 21 20 22

day 14

- 28 males MEAN 53.9 56.0 54.5 50.9

S.D. 6.98 4.43 6.80 3.72

N 20 21 20 22

females MEAN 48.4 50.3 48.7 47.4

S.D. 4.44 3.28 4.60 3.60

N 20 21 19 22

males+females MEAN 51.3 53.0 52.2 49.2

S.D. 5.52 3.71 6.75 3.49

N 20 21 20 22

day 21

- 28 males MEAN 38.6 39.2 38.1 35.8*

S.D. 4.56 2.85 3.79 2.04

N 20 21 20 22

females MEAN 33.4 33.8 32.9 32.2

S.D. 3.00 1.88 2.65 1.92

N 20 21 19 22

males+females MEAN 36.1 36.3 35.8 34 .1

S.D. 3.60 2.27 3.86 1.93

N 20 21 20 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0523

- JUN

- 2009 11:22

Huntingdon Life Sciences 00

- 4206 PAGE 90

TABLE 19

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26

TH YMUS N 1 3 4 4

------

DISCOLORED FOCI N 1 3 4 4

% 3.8 11.5 15.4 15.4

LUNGS N 4 8 10 12

-----

DISCOLORED FOCI N 4 8 10 12

% 15.4 30.8 38.5 46.2

ABDOMINAL CAVITY N 1 0 0 0

----------------

DISCOLORED LYMPH NODE(S) N 1 0 0 0

% 3.8 0.0 0.0 0.0

KIDNEY N 3 3 5 4

------

DILATED RENAL PELVIS N 2 3 5 4

% 7.7 11.5 19.2 15.4

IRREGULAR SURFACE N 1 0 0 0

% 3.8 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JUN

- 2009 11:22

Huntingdon Life Sciences 00

- 4206 PAGE 91

TABLE 19

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26

BLADDER N 0 1 0 0

-------

CALCULI N 0 1 0 0

% 0.0 3.8 0.0 0.0

GONADS N 1 0 0 1

------

TESTE(S) SMALL N 1 0 0 0

% 3.8 0.0 0.0 0.0

TESTE(S) SOFT N 1 0 0 0

% 3.8 0.0 0.0 0.0

EPIDIDYMIS(IDES) SMALL N 1 0 0 0

% 3.8 0.0 0.0 0.0

UNDESCENDED TESTE(S) N 0 0 0 1

% 0.0 0.0 0.0 3.8

-----------------------------------------------------------------------------------------------------------------------------------

N o statistically significant differences23

- JUN

- 2009 11:22

Huntingdon Life Sciences 00

- 4206 PAGE 92

TABLE 19

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATI ON WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

---------------------------------------------------------------------------------------------------------------------------- -------

FEMALES N 26 26 26 26

THYMUS N 1 0 0 1

------

DISCOLORED FOCI N 1 0 0 1

% 3.8 0.0 0.0 3.8

LUNGS N 2 9 4 14

-----

DISCOLORED FOCI N 2 9 4 14**

% 7.7 34.6 15.4 53.8

ABDOMINAL CAVITY N 0 1 0 0

----------------

MASS N 0 1 0 0

% 0.0 3.8 0.0 0.0

LIVER N 2 0 0 0

-----

ADHESION N 2 0 0 0

% 7.7 0.0 0.0 0.0

MASS N 1 0 0 0

% 3.8 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0123

- JUN

- 2009 11:22

Huntingdon Life Sciences 00

- 4206 PAGE 93

TABLE 19

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

FEMALES N 26 26 26 26

KIDNEY N 1 0 0 1

------

DILATED RENAL PELVIS N 0 0 0 1

% 0.0 0.0 0.0 3.8

MASS N 1 0 0 0

% 3.8 0.0 0.0 0.0

ADRENALS N 1 1 1 0

--------

DISCOLORED ADRENALS N 1 1 0 0

% 3.8 3.8 0.0 0.0

DISCOLORED FOCI N 0 0 1 0

% 0.0 0.0 3.8 0.0

GONADS N 1 0 0 0

------

FLUID

- FILLED VAGINA N 1 0 0 0

% 3.8 0.0 0.0 0.0

UTERUS N 1 0 0 0

------

FETUS(ES) IN UTERUS N 1 0 0 0

% 3.8 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences23

- JU N- 2009 11:22

Huntingdon Life Sciences 00

- 4206 PAGE 94

TABLE 19

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

---------------------------------------------------------------------------------------------------------------------------------- -

FEMALES N 26 26 26 26

OVARY N 1 0 0 0

-----

CYST(S) N 1 0 0 0

% 3.8 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically sig nificant differences23

- JUN

- 2009 12:49

Huntingdon Life Sciences 00

- 4206 PAGE 95

TABLE 20

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF NECROPSY OBSERVATIONS FOR DEAD PUPS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 2 4 4 5

Pups Evaluated N 2 4 6 11

GROSS EXAM

----------

Litter Incidence N 1 0 0 2

Pup Incidence N 1 0 0 3

AUTOLYSIS

Pup Incidence N 1 0 0 3

% 50.0 0.0 0.0 27.3

Litter Incidence N 1 0 0 2

% 50.0 0.0 0.0 40.0

CANNIBALIZED

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 9.1

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 20.0

LUNGS

-----

Litter Incidence N 0 3 2 2

Pup Incidence N 0 3 3 6

LUNG FLOATATION TEST - FOUND DEAD

Pup Incidence N 0 3 3 6

% 0.0 75.0 50.0 54.5

Litter Incidence N 0 3 2 2

% 0.0 75.0 50.0 40.0

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 12:49

Huntingdon Life Sciences 00

- 4206 PAGE 96

TABLE 20

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF NECROPSY OBSERVATIONS FOR DEAD PUPS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOS E LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters E valuated N 2 4 4 5

Pups Evaluated N 2 4 6 11

STOMACH

------ -

Litter Incidence N 1 4 4 4

Pup Incidence N 1 4 6 8

MILK IN STOM ACH

Pup Incidence N 1 2 3 0

% 50.0 50.0 50.0 0.0

Litter Incidence N 1 2 3 0

% 50.0 50.0 75.0 0.0

NO MILK IN STOMACH

Pup Incidence N 0 2 3 8

% 0.0 50.0 50.0 72.7

Litter Incidence N 0 2 2 4

% 0.0 50.0 50.0 80.0

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 12:51

Huntingdon Life Sciences 00

- 4206 PAGE 97

TABLE 20

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF NECROPSY OBSERVATIONS FOR STILLBORN P UPS a

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 1 5 5 0

Pups Evaluated N 1 5 12 0

GROSS EXAM

----------

Litter Incidence N 0 1 2 0

Pup Incidence N 0 1 2 0

AUTOLYSIS

Pup Incidence N 0 1 2

% 0.0 20.0 16.7

Litter Incidence N 0 1 2

% 0.0 2 0 .0 40.0

CANNIBALIZED

Pup Incidence N 0 1 2

% 0.0 2 0 .0 16.7

Litter Incidence N 0 1 1

% 0.0 2 0 .0 20 .0

SEX UNDETERMINED

Pup Incidence N 0 1 2

% 0.0 2 0 .0 16.7

Litter Incidence N 0 1 1

% 0.0 2 0 .0 20 .0

LUNGS

-----

Litter Incidence N 1 3 4 0

Pup Incidence N 1 3 8 0

LUNG FLOATATION TEST - STILLBORN

Pup Incidence N 1 3 8

% 100.0 60 .0 66.7

Litter Incidence N 1 3 4

% 100.0 60 .0 80.0

-----------------------------------------------------------------------------------------------------------------------------------

a Includes autolytic or cannibalized pups for which a lung floatation test could not be performed.23

- JUN

- 2009 12:51

Huntingdon Life Sciences 00

- 4206 PAGE 98

TABLE 20

GASOL INE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF NECROPSY OBSERVATIONS FOR STILLBORN PUPS a

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II I II IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 1 5 5 0

Pups Evaluated N 1 5 12 0

STOMACH

-------

Litter Incidence N 1 3 4 0

Pup Incidence N 1 3 8 0

NO MILK IN STOMACH

Pup Incidence N 1 3 8

% 100.0 60 .0 66.7

Litter Incidence N 1 3 4

% 100.0 60 .0 80.0

-----------------------------------------------------------------------------------------------------------------------------------

a Includes autolytic or cannibalized pups for whi ch a lung floatation test could not be performed.23

- JUN

- 2009 12:53

Huntingdon Life Sciences 00

- 4206 PAGE 99

TABLE 20

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTIO N TOXICITY STUDY IN RATS

SUMMARY OF NECROPSY OBSERVATIONS FOR CULLED PUPS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 20 17 18 20

Pups Evaluated N 84 54 76 78

TOTAL PUPS CULLED OBSERVATIONS

N 0 0 0 0

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 12:55

Huntingdon Life Sciences 00

- 4206 PAGE 100

TABLE 20

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF NECROPSY OBSERVA TIONS FOR CANNIBALIZED PUPS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 0 0 2 0

Pups Evaluated N 0 0 6 0

GROSS EXAM

----------

Litter Incidence N 0 0 2 0

Pup Incidence N 0 0 6 0

CANNIBALIZED

Pup Incidence N 6

% 100.0

Litter Incidence N 2

% 100.0

SEX UNDETERMINED

Pup Incidence N 6

% 100.0

Litter Incidence N 2

% 100.0

STOMACH

-------

Litter Incidence N 0 0 1 0

Pup Incidence N 0 0 5 0

MILK IN STOMACH UNDETERMINED

Pup Incidence N 5

% 83.3

Litter Incidence N 1

% 50.0

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 12:56

Huntingdon Life Sciences 00

- 4206 PAGE 101

TABLE 20

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHA LATION REPRODUCTION TOXICITY STUDY IN RATS

SUMARY OF NECROPSY OBSERVATIONS FOR WEANLING PUPS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 20 21 20 22

Pups Evaluated N 120 123 117 132

KIDNEY

------

Litter Incidence N 1 0 4 2

Pup Incidence N 1 0 5 2

DILATED RENAL PELVIS (PAPILLA REDUCED)

Pup Incidence N 1 0 5 2

% 0.8 0.0 4.3 1.5

Litter Incidence N 1 0 4 2

% 5.0 0.0 20.0 9.1

GONADS

------

Litter Incidence N 2 4 2 1

Pup Incidence N 2 4 3 1

UNDESCENDED TEST E(S)

Pup Incidence N 2 3 3 1

% 1.7 2.4 2.6 0.8

Litter Incidence N 2 3 2 1

% 10.0 14.3 10.0 4.5

SMALL TESTE(S)

Pup Incidence N 0 1 0 0

% 0.0 0.8 0.0 0.0

Litter Incidence N 0 1 0 0

% 0.0 4.8 0.0 0.0

SMALL EPIDIDYMIS(IDES)

Pup Incidence N 0 1 0 0

% 0.0 0.8 0.0 0.0

Litter Incidence N 0 1 0 0

% 0.0 4.8 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

# Huntingdon Life Sciences 00-4206 Page 102

211-TBA-1G Final Report

Summary of Parental Organ Weights

Preface Table 21

Table of Contents

Summary of absolute organ weights ................................................................................103

Summary of organ to body weight ratio ..........................................................................107

Summary of organ to br ain weight ratio ..........................................................................111

Key to Abbreviations:

g = Grams

SEM. VES & CO. GL. = Seminal Vesicles and Coagulating Gland

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m 3



# Group II - 2000 mg/m 3



# Group III - 10000 mg/m 3



# Group IV - 20000 mg/m 323-JUN-2009 13:04Huntingdon Life Sciences 00-4206 PAGE 103TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ABSOLUTE ORGAN WEIGHTSMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 569 576 556 536*S.D. 57.0 41.8 52.2 35.1N 26 26 26 26TESTIS - RIGHT g MEAN 1.7925 1.7901 1.7888 1.7910S.D. .13517 .15635 .11505 .16589N 26 26 26 26TESTIS - LEFT g MEAN 1.7624 1.7816 1.7859 1.7944S.D. .21606 .14279 .10812 .16313N 26 26 26 26TESTES g MEAN 3.5384 3.5596 3.5631 3.5752S.D. .31536 .29683 .21703 .32569N 26 26 26 26EPIDIDYMIS RIGHT g MEAN 0.7381 0.7569 0.7505 0.7086S.D. .06263 .06071 .07239 .05080N 26 26 26 26EPIDIDYMIS LEFT g MEAN 0.7234 0.7505 0.7388 0.6980S.D. .07847 .06446 .07537 .05588N 26 26 25 26EPIDIDYMIDES g MEAN 1.4520 1.5015 1.4805 1.3983S.D. .12840 .12046 .14157 .09845N 26 26 25 26PROSTATE g MEAN 1.0457 1.1186 1.0995 1.1301S.D. .16987 .18424 .11776 .22795N 26 26 26 26SEM.VES & CO.GL. g MEAN 2.2416 2.0959 2.3462 2.1574S.D. .31168 .27162 .33621 .35744N 26 26 26 26-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.0523-JUN-2009 13:04Huntingdon Life Sciences 00-4206 PAGE 104TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ABSOLUTE ORGAN WEIGHTSMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------ADRENALS g MEAN 0.0663 0.0656 0.0691 0.0716S.D. .00764 .01163 .01139 .01019N 26 26 26 26BRAIN g MEAN 2.1416 2.1758 2.1519 2.1151S.D. .09677 .09573 .08358 .08256N 26 26 26 26KIDNEYS g MEAN 4.1621 4.3807 4.8115** 4.8779**S.D. .46126 .46640 .46759 .44047N 26 26 26 26LIVER g MEAN 20.116 20.907 20.804 21.925S.D. 2.2338 2.4937 2.6955 2.8147N 26 26 26 26LUNGS g MEAN 1.9724 2.0157 1.8955 1.8640S.D. .21622 .23339 .18587 .17998N 26 26 26 26PITUITARY g MEAN 0.0126 0.0129 0.0120 0.0129S.D. .00170 .00167 .00261 .00191N 26 26 26 26SPLEEN g MEAN 0.8980 0.8472 0.8148* 0.7545**S.D. .14468 .11408 .12958 .08771N 26 26 26 26-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.0123-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 105TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ABSOLUTE ORGAN WEIGHTSFEMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 330 329 329 325S.D. 23.3 25.6 18.6 23.2N 20 21 20 22OVARY RIGHT g MEAN 0.0624 0.0614 0.0640 0.0538S.D. .01422 .01367 .01260 .01214N 20 21 20 22OVARY LEFT g MEAN 0.0628 0.0552 0.0604 0.0539S.D. .02558 .01187 .01423 .01246N 20 21 20 22OVARIES g MEAN 0.1229 0.1152 0.1238 0.1097S.D. .03215 .02283 .02447 .01643N 20 21 20 22UTERUS/OVIDUCTS g MEAN 0.7183 0.6427 0.6228 0.6791S.D. .19003 .19923 .19070 .22233N 20 21 20 22ADRENALS g MEAN 0.0832 0.0801 0.0809 0.1050S.D. .00741 .01310 .01201 .10360N 20 21 20 22BRAIN g MEAN 1.9762 1.9704 1.9640 1.9685S.D. .12457 .07731 .07489 .08655N 20 21 20 22KIDNEYS g MEAN 3.3461 2.8058 2.8555 2.9382S.D. 2.6250 .32166 .15930 .24147N 20 21 20 22-----------------------------------------------------------------------------------------------------------------------------------No statistically significant differences23-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 106TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ABSOLUTE ORGAN WEIGHTSFEMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------LIVER g MEAN 15.244 15.752 15.573 16.410S.D. 1.7866 2.2077 1.6969 1.9907N 20 21 20 22LUNGS g MEAN 1.5451 1.5705 1.5477 1.5193S.D. .17124 .13311 .11356 .11689N 20 21 20 22PITUITARY g MEAN 0.0141 0.0150 0.0130 0.0135S.D. .00241 .00297 .00389 .00237N 20 21 20 22SPLEEN g MEAN 0.7124 0.6256* 0.6363* 0.6030**S.D. .10949 .10738 .09522 .07556N 20 21 20 22-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.0123-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 107TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIOMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 569 576 556 536*S.D. 57.0 41.8 52.2 35.1N 26 26 26 26TESTIS - RIGHT Ratio MEAN 0.0032 0.0031 0.0032 0.0034S.D. .00029 .00028 .00040 .00034N 26 26 26 26TESTIS - LEFT Ratio MEAN 0.0031 0.0031 0.0032 0.0034*S.D. .00043 .00026 .00040 .00033N 26 26 26 26TESTES Ratio MEAN 0.0063 0.0062 0.0065 0.0067S.D. .00067 .00054 .00079 .00066N 26 26 26 26EPIDIDYMIS RIGHT Ratio MEAN 0.0013 0.0013 0.0014 0.0013S.D. .00012 .00011 .00016 .00010N 26 26 26 26EPIDIDYMIS LEFT Ratio MEAN 0.0013 0.0013 0.0013 0.0013S.D. .00015 .00010 .00014 .00012N 26 26 25 26EPIDIDYMIDES Ratio MEAN 0.0026 0.0026 0.0027 0.0026S.D. .00025 .00021 .00030 .00021N 26 26 25 26PROSTATE Ratio MEAN 0.0018 0.0019 0.0020 0.0021**S.D. .00027 .00032 .00021 .00044N 26 26 26 26SEM.VES & CO.GL. Ratio MEAN 0.0040 0.0037 0.0042 0.0041S.D. .00064 .00052 .00047 .00082N 26 26 26 26-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.0523-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 108TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIOMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------ADRENALS Ratio MEAN 0.0001 0.0001 0.0001 0.0001**S.D. .00002 .00002 .00002 .00002N 26 26 26 26BRAIN Ratio MEAN 0.0038 0.0038 0.0039 0.0040S.D. .00031 .00028 .00036 .00025N 26 26 26 26KIDNEYS Ratio MEAN 0.0073 0.0076 0.0087** 0.0091**S.D. .00051 .00070 .00060 .00078N 26 26 26 26LIVER Ratio MEAN 0.0354 0.0363 0.0374 0.0410**S.D. .00277 .00301 .00279 .00537N 26 26 26 26LUNGS Ratio MEAN 0.0035 0.0035 0.0034 0.0035S.D. .00028 .00041 .00031 .00031N 26 26 26 26PITUITARY Ratio MEAN 0.0000 0.0000 0.0000 0.0000S.D. .00000 .00000 .00000 .00000N 26 26 26 26SPLEEN Ratio MEAN 0.0016 0.0015 0.0015 0.0014**S.D. .00019 .00018 .00019 .00017N 26 26 26 26-----------------------------------------------------------------------------------------------------------------------------------Statistical key: ** = p<0.0123-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 109TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIOFEMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------FINAL BODY WEIGHT g MEAN 330 329 329 325S.D. 23.3 25.6 18.6 23.2N 20 21 20 22OVARY RIGHT Ratio MEAN 0.0002 0.0002 0.0002 0.0002S.D. .00005 .00004 .00004 .00004N 20 21 20 22OVARY LEFT Ratio MEAN 0.0002 0.0002 0.0002 0.0002S.D. .00007 .00004 .00004 .00004N 20 21 20 22OVARIES Ratio MEAN 0.0004 0.0004 0.0004 0.0003S.D. .00010 .00007 .00007 .00005N 20 21 20 22UTERUS/OVIDUCTS Ratio MEAN 0.0022 0.0020 0.0019 0.0021S.D. .00063 .00066 .00066 .00074N 20 21 20 22ADRENALS Ratio MEAN 0.0003 0.0002 0.0002 0.0003S.D. .00003 .00004 .00004 .00033N 20 21 20 22BRAIN Ratio MEAN 0.0060 0.0060 0.0060 0.0061S.D. .00031 .00045 .00031 .00048N 20 21 20 22KIDNEYS Ratio MEAN 0.0101 0.0085 0.0087 0.0091S.D. .00746 .00077 .00061 .00063N 20 21 20 22-----------------------------------------------------------------------------------------------------------------------------------No statistically significant differences23-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 110TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIOFEMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------LIVER Ratio MEAN 0.0461 0.0477 0.0473 0.0504**S.D. .00326 .00449 .00388 .00355N 20 21 20 22LUNGS Ratio MEAN 0.0047 0.0048 0.0047 0.0047S.D. .00047 .00034 .00027 .00033N 20 21 20 22PITUITARY Ratio MEAN 0.0000 0.0000 0.0000 0.0000S.D. .00001 .00001 .00001 .00001N 20 21 20 22SPLEEN Ratio MEAN 0.0022 0.0019** 0.0019* 0.0019**S.D. .00030 .00024 .00024 .00024N 20 21 20 22-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.0123-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 111TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIOMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------TESTIS - RIGHT Ratio MEAN 0.8373 0.8231 0.8315 0.8483S.D. .05536 .06740 .04600 .08856N 26 26 26 26TESTIS - LEFT Ratio MEAN 0.8233 0.8194 0.8303 0.8498S.D. .09647 .06319 .04547 .08611N 26 26 26 26TESTES Ratio MEAN 1.6529 1.6370 1.6564 1.6933S.D. .13469 .12900 .08857 .17344N 26 26 26 26EPIDIDYMIS RIGHT Ratio MEAN 0.3448 0.3479 0.3487 0.3351S.D. .02614 .02341 .02959 .02184N 26 26 26 26EPIDIDYMIS LEFT Ratio MEAN 0.3379 0.3451 0.3432 0.3301S.D. .03330 .02756 .03074 .02423N 26 26 25 26EPIDIDYMIDES Ratio MEAN 0.6782 0.6904 0.6877 0.6613S.D. .05291 .05007 .05684 .04200N 26 26 25 26PROSTATE Ratio MEAN 0.4878 0.5141 0.5119 0.5361S.D. .07167 .08093 .06030 .11587N 26 26 26 26SEM.VES & CO.GL. Ratio MEAN 1.0491 0.9629 1.0922 1.0234S.D. .15495 .11441 .16551 .18901N 26 26 26 26-----------------------------------------------------------------------------------------------------------------------------------No statistically significant differences23-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 112TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIOMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------ADRENALS Ratio MEAN 0.0310 0.0301 0.0321 0.0339S.D. .00329 .00508 .00482 .00490N 26 26 26 26KIDNEYS Ratio MEAN 1.9417 2.0134 2.2378** 2.3098**S.D. .17306 .19453 .22671 .23028N 26 26 26 26LIVER Ratio MEAN 9.3948 9.6069 9.6641 10.366**S.D. .97181 1.0286 1.1604 1.2671N 26 26 26 26LUNGS Ratio MEAN 0.9201 0.9259 0.8800 0.8826S.D. .08183 .09174 .06760 .09290N 26 26 26 26PITUITARY Ratio MEAN 0.0059 0.0059 0.0056 0.0061S.D. .00066 .00085 .00126 .00096N 26 26 26 26SPLEEN Ratio MEAN 0.4184 0.3892 0.3784* 0.3570**S.D. .05797 .04791 .05577 .04239N 26 26 26 26-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.0123-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 113TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIOFEMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------OVARY RIGHT Ratio MEAN 0.0316 0.0312 0.0326 0.0274S.D. .00726 .00689 .00613 .00620N 20 21 20 22OVARY LEFT Ratio MEAN 0.0317 0.0280 0.0307 0.0274S.D. .01216 .00595 .00687 .00620N 20 21 20 22OVARIES Ratio MEAN 0.0622 0.0585 0.0630 0.0557S.D. .01537 .01124 .01170 .00798N 20 21 20 22UTERUS/OVIDUCTS Ratio MEAN 0.3670 0.3260 0.3169 0.3457S.D. .10933 .09837 .09618 .11551N 20 21 20 22ADRENALS Ratio MEAN 0.0423 0.0407 0.0412 0.0541S.D. .00500 .00649 .00594 .05638N 20 21 20 22KIDNEYS Ratio MEAN 1.6996 1.4245 1.4549 1.4950S.D. 1.3591 .15917 .08203 .13425N 20 21 20 22LIVER Ratio MEAN 7.7172 8.0030 7.9290 8.3431S.D. .79859 1.1485 .81625 1.0011N 20 21 20 22LUNGS Ratio MEAN 0.7809 0.7983 0.7886 0.7721S.D. .06068 .07763 .05766 .05379N 20 21 20 22-----------------------------------------------------------------------------------------------------------------------------------No statistically significant differences23-JUN-2009 13:06Huntingdon Life Sciences 00-4206 PAGE 114TABLE 21GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIOFEMALES-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------PITUITARY Ratio MEAN 0.0072 0.0076 0.0067 0.0069S.D. .00141 .00155 .00203 .00129N 20 21 20 22SPLEEN Ratio MEAN 0.3605 0.3180* 0.3238 0.3070**S.D. .05207 .05668 .04550 .04055N 20 21 20 22-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01

# Huntingdon Life Sciences 00-4206 Page 115

211-TBA-1G Final Report

Summary of Pup Organ Weight Data

Preface Table 22

Table of Contents

Pup Organ Weights in Grams ..........................................................................................116

Pup Organ Weight to Body Weight Ratio .......................................................................117

Pup Organ Weight to Br ain Weight Ratio .......................................................................11823-JUN-2009 14:13Huntingdon Life Sciences 00-4206 PAGE 116TABLE 22GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF PUP ORGAN WEIGHT DATAPUP ORGAN WEIGHTS IN GRAMS-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------BRAIN MEAN 1.5335 1.5409 1.5486 1.5004S.D. .08286 .06367 .07232 .05175N 20 21 20 22of Male Pups MEAN 1.5485 1.5762 1.5718 1.5274S.D. .09903 .06840 .08513 .05409N 20 21 20 22of Female Pups MEAN 1.5185 1.5056 1.5175 1.4734S.D. .08048 .07767 .06365 .07842N 20 21 19 22SPLEEN MEAN 0.3202 0.3194 0.3199 0.2785**S.D. .03742 .03289 .05166 .03192N 20 21 20 22of Male Pups MEAN 0.3380 0.3348 0.3333 0.2934*S.D. .05149 .04731 .05394 .04229N 20 21 20 22of Female Pups MEAN 0.3023 0.3041 0.2985 0.2636*S.D. .04396 .03927 .04979 .03955N 20 21 19 22THYMUS MEAN 0.3527 0.3487 0.3749 0.3389S.D. .05634 .03975 .06559 .04169N 20 21 20 22of Male Pups MEAN 0.3570 0.3356 0.3718 0.3279S.D. .06910 .05711 .06407 .04576N 20 21 20 22of Female Pups MEAN 0.3484 0.3618 0.3682 0.3500S.D. .05876 .04392 .06318 .06116N 20 21 19 22-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.0123-JUN-2009 14:13Huntingdon Life Sciences 00-4206 PAGE 117TABLE 22GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF PUP ORGAN WEIGHT DATAPUP ORGAN WEIGHT TO BODY WEIGHT RATIO-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------BRAIN MEAN 2.0117 1.9891 2.0493 2.1030S.D. .18356 .14575 .18269 .12760N 20 21 20 22of Male Pups MEAN 1.9522 1.9402 2.0129 2.0364S.D. .21156 .17893 .17334 .15630N 20 21 20 22of Female Pups MEAN 2.0712 2.0379 2.1095 2.1695S.D. .18942 .15056 .18912 .19698N 20 21 19 22SPLEEN MEAN 0.4169 0.4114 0.4183 0.3879S.D. .04349 .05104 .04353 .03854N 20 21 20 22of Male Pups MEAN 0.4230 0.4125 0.4235 0.3895S.D. .05278 .07058 .05118 .05034N 20 21 20 22of Female Pups MEAN 0.4107 0.4103 0.4115 0.3863S.D. .05451 .04627 .05162 .05555N 20 21 19 22THYMUS MEAN 0.4572 0.4504 0.4911 0.4723S.D. .04710 .05477 .05135 .03781N 20 21 20 22of Male Pups MEAN 0.4443 0.4123 0.4731 0.4347S.D. .06135 .07036 .06382 .04554N 20 21 20 22of Female Pups MEAN 0.4700 0.4885 0.5070 0.5100S.D. .05496 .05513 .05892 .06224N 20 21 19 22-----------------------------------------------------------------------------------------------------------------------------------No statistically significant differences23-JUN-2009 14:14Huntingdon Life Sciences 00-4206 PAGE 118TABLE 22GASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSSUMMARY OF PUP ORGAN WEIGHT DATAPUP ORGAN WEIGHT TO BRAIN WEIGHT RATIO-----------------------------------------------------------------------------------------------------------------------------------DOSE GROUP I II III IVDOSE LEVEL (MG/M3) 0 2000 10000 20000-----------------------------------------------------------------------------------------------------------------------------------SPLEEN MEAN 20.878 20.711 20.622 18.523**S.D. 2.3979 1.8125 2.9267 1.8402N 20 21 20 22of Male Pups MEAN 21.822 21.266 21.199 19.202*S.D. 3.0676 3.0595 3.1438 2.5890N 20 21 20 22of Female Pups MEAN 19.934 20.156 19.671 17.844*S.D. 2.9284 1.9680 3.1341 2.1674N 20 21 19 22THYMUS MEAN 22.953 22.699 24.181 22.553S.D. 3.2980 2.6458 3.6712 2.4317N 20 21 20 22of Male Pups MEAN 22.987 21.368 23.639 21.438S.D. 3.9137 3.9079 3.6607 2.6464N 20 21 20 22of Female Pups MEAN 22.918 24.029 24.252 23.669S.D. 3.6502 2.6330 3.9064 3.4772N 20 21 19 22-----------------------------------------------------------------------------------------------------------------------------------Statistical key: * = p<0.05 ** = p<0.01

# Huntingdon Life Sciences 00-4206 Pa ge 119

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Lesion Incidence Summary

with Expanded Severity Levels

(P0 Generation)

Preface Table 23

Key to Abbreviations

Gl = Gland

Nad = No abnormal diagnoses

Ctls = Controls (Group I)

LN = Lymph Node

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m 3



# Group IV - 20000 mg/m 3Page 120Page 121Page 122Page 123Page 124

# Huntingdon Life Sciences 00-4206 Page 125

211- TBA -1G Final Report

Inhalation Report Appendix A

TABLE OF CONTENTS

1. INTRODUCTION ..............................................................................................126

2. MATERIALS AND METHODS

2.1. Husbandry During Exposure Periods .......................................................126

2.2. Test Substance Administration and Chamber Operation .........................127

2.3. Exposure Concentration Determination ...................................................131

3. TABLES

I. Summary of In -Chamber Observations ...................................................133

II. Chamber Monitoring Results ...................................................................142

III. Equ ipment List .........................................................................................164

IV. Chamber Distribution Records ................................................................167

V. Miran Calibration .....................................................................................169

VI. Testing Room Environmental Monitoring ...............................................172

4. FIGURES

I. Chamber Generation System and Whole-Body Exposure

Chamber – Group I ..............................................................................173

II. Chamber Generation System and Whole-Body Exposure

Chamber

– Groups II, III, IV ...............................................................174

# Huntingdon Life Sciences 00-4206 Page 126

211- TBA -1G Final Report

Inhalation Report Appendix A

1. INTRODUCTION

This appendix presents the methodology for exposure atmosphere generation

monitoring and results.

2. MATERIALS AND METHOD S

2.1. HUSBANDRY DURING EXPOSURE PERIODS

2.1.1. HOUSING

Animals were individually housed in stainless steel, wire mesh

cages within a 1.5 m 3 

# glass and stainless steel whole -body

exposure chamber. The placement of the animals in the whole

body exposure chamber was rotated once weekly to ensure uniform

exposure of the animals. A description of the animal rotation is

included in the raw data.

2.1.2. FEED

None was provided during exposure.

2.1.3. WATER

None was provided during exposure.

2.1.4. ENVIRONMENTAL CONDITIONS

Chamber temperature and relative humidity every half

- hour during

exposure and maintained, to the maximum extent possible, within

the ranges presente d below. Excursions outside the specified range

did not affect the integrity of the study.

Temperature

Desired: 20 to 24 ° C

Actual: 18 to 28 ° C

Average: 21 to 27 ° C

# Huntingdon Life Sciences 00-4206 Page 127

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Inhalation Report Appendix A

Relative Humidity

Desired: 40 to 60%

Actual: 25 to 82%

Average: 30 to 68%

2.2. TEST SUBSTANCE ADMINISTRATION AND C HAMBER

OPERATIONS

2.2.1. ROUTE OF ADMINISTRAT ION

Inhalation via whole-body exposures

2.2.2. TEST SUBSTANCE ADMINISTRATION

The test substance was administered as a vapor in the breathing air

of the animals. The test atmosphere was generated by an

ap propriate procedure determined during the pre

- study trials. The

trials were performed to evaluate the optimal set of conditions and

equipment to generate a stable atmosphere at the target exposure

levels and maintain uniform conditions throughout the expo sure

chambers.

2.2.3. JUSTIFICATION FOR ROUTE OF ADMINISTRATIO N

The inhalation route is one of the potential routes of human

exposure to this test substance and is the route specified in the

referenced US EPA 79.61 guidelines and US OPPTS 870.3800

guidelines.

2.2.4. TAR GET EXPOSURE LEVELS

Group I - 0 mg/m 3



# Group II - 2,000 mg/m 3



# Group III - 10,000 mg/m 3



# Group IV - 20,000 mg/m 3

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Inhalation Report Appendix A

2.2.5. JUSTIFICATION FOR EX POSURE LEVEL SELECTI ON

Exposure levels were selected by the Sponsor, based on results

from a 13

- week inhalation study conduct ed at the Testing Facility,

that utilized this test substance in rats (00

- 6131). The exposure

levels were also selected based on the lower flammable limits of

the test substance.

2.2.6. FREQUENCY OF ADMINISTRATION

Frequency

P 0 

# males and females received 70 cons ecutive days (ten weeks) of

exposure prior to mating for six hours/day. P 0 

# males and females

continued to be exposed daily throughout a 14

- day cohabitation

period. The mated females continued to be exposed daily from

Day 0 through 19 of gestation. Beginning on Day 5 of lactation,

nursing P 0 

# females were exposed daily until weaning of the F 1 

# offspring on Day 28. P 0 

# females with no confirmed day of mating

but with evidence of pregnancy (weight gain) continued to be

exposed until the presumed Day 19 of gest ation. P 0 

# females with

no confirmed day of mating and with no evidence of pregnancy

(weight gain) were exposed for 25 days following completion of

the mating period and then euthanized. P 0 

# females with a

confirmed day of mating that did not deliver were euthanized on

presumed Day 25 of gestation.

P 0 

# males were exposed daily until euthanasia, after the last F 1



# litters were delivered.

2.2.7. DURATION

The test substance was administered for 6 hours/day during all

segments of the study.

# Huntingdon Life Sciences 00-4206 Page 129

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Inhalation Report Appendix A

2.2.8. EXPOSURE PROCEDURE AND CHAMBER OPERATIONS

Group I

Houseline nitrogen was delivered from a regulator with a

backpressure gauge via 1⁄4” tubing to a flowmeter regulated by a

metering valve. This nitrogen flow (~ 20 Lpm) was then directed

into the turret of the 1.5 m 3 

# glass and stainless steel exposure

chamber where it was mixed with room air as it was drawn into the

chamber. This nitrogen flow simulated the generation nitrogen

flow for Groups II, III, and IV.

Groups II, III and IV

Houseline nitrogen was delivered from a regulator with a

backpressure gauge through a stainless steel fitting to create three

flow systems: the test substance pressurization flow, the purge flow

and the volatilization flow.

The nitrogen for the test substance pressurization flow was directed

via 1⁄4” tubing throu gh a metering valve, attached to a backpressure

gauge, into the vapor inlet valve of the test substance cylinder. The

metering valve was used to adjust and maintain the pressure within

the cylinder. From the pressurized cylinder, the test substance

flowe d from the liquid outlet valve through a quick -disconnect

fitting and through a filter to prevent equipment contamination.

From the filter, the test substance flowed to a liquid flowmeter via

1 

# / 8 

# ” tubing. The outlet of the flowmeter was regulated by a bu ilt -in

metering valve. From this metering valve, the test substance

flowed via 1 

# / 8 

# ” tubing onto the glass helix of a counter current

volatilization chamber. The glass helix was heated by a nichrome

wire, which was controlled by an autotransformer and was inserted

in the center of the glass tube that supported the helix.

The nitrogen for the purge flow system was directed, via 1⁄4” tubing

to a flowmeter regulated by a metering valve. The purge nitrogen

was delivered via 1 

# / 8 

# ” tubing to the bottom of the tube containing

the nichrome wire. This nitrogen flow continuously purged the

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Inhalation Report Appendix A

area surrounding the nichrome wire within the tube, thereby

protecting the wire from oxidation.

The nitrogen for the volatilization system was directed via 1⁄4”

tubing to a flowmeter regulated by a metering valve. From the

flowmeter, the volatilization nitrogen (~ 18 Lpm for Group II and

~ 20 Lpm for Groups III and IV ) flowed via 1⁄4” tubing to a ball

and socket joint at the bottom of the volatilization chamber. This

nitrogen flowed up through the volatilization chamber passing over

the coil and volatilizing the test substance. The pressure within the

counter- current volatilization chamber was maintained slightly

negative to the room and was monitored with a pressure gauge.

This test substance laden nitrogen exited the top of the

volatilization chamber through a glass elbow, which directed the

flow, via 1⁄2” tubing, to the turret of 1.5 m 3 

# glass and stainless steel

exposure chamber. As the test substance laden nitrogen was drawn

into t he chamber, it was mixed with room air.

The whole

- body exposure chambers each had a volume of

approximately 1500 Liters (1.5 m 3 

# ). Each chamber was operated at

a minimum flow rate of 300 Liters per minute. The final airflow

was set to provide at least one air change (calculated by dividing

the chamber volume by the airflow rate) in 5.0 minutes (12 air

changes/hour) and a T 99 

# equilibrium time (calculated by

multiplying the air change by the exponential factor 4.6) of at most

23 minutes:

Group Airflow Rate A ir Change T 99 

# (Lpm) (min) (min)

I 317 4.7 22

II 312 4.8 22

III 320 4.7 22

IV 303 5.0 23

This chamber size and airflow rate was considered adequate to

maintain the oxygen level at least 19% and the animal -loading

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Inhalation Report Appendix A

factor below 5%. Chamber temperature, humidity, airflow rate and

static pressure were monitored continuously and recorded every

30 minutes during exposure. At the end of the 6

- hour exposure, all

animals remained in the chamber for a minimum of 30 minutes.

During this time the chamber was o perated at approximately the

same flow rate using clean air only. The chambers were exhausted

through the in housed filtering system, which consisted of a coarse

filter, a HEPA filter and activated charcoal.

See Chamber Figures I and II and Table III for equipment details.

2.3. EXPOSURE CONCENTRATION DETERMINATION

2.3.1. NOMINAL COCENTRATION

A nominal exposure concentration was calculated. The flow of air

through the chamber was monitored using appropriate calibrated

equipment. The test substance consumed (weight di fference of the

test substance cylinder) during the exposure (mg) was divided by

the total volume of air (m 3 

# ) passing through the chamber

(volumetric flow rate times total exposure time) to give the

nominal concentration (mg/m 3 

# ).

2.3.2. CHAMBER SAMPLING

During each exposure, measurements of airborne concentrations

were performed in the animals’ breathing zone at least 4 times

using an appropriate sampling procedure (~ 20 Lpm) and Infra

- Red

Spectrophotometric (IR) analytical method. Also, one charcoal

tube sample per chamber per week was collected (at ~ 20 Lpm) and

was analyzed by gas chromatography (GC) to characterize at least

10 major components (comprising at least 80% by weight of the

test substance) to show test substance stability and comparison

between the neat liquid test substance and the vaporized test

atmospheres.

See Table III for equipment details. See Appendix B for Analytical

Report.

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2.3.3. PARTICLE SIZE DISTRIBUTION

During each week of exposure, particle size determinations were

performed using a TSI A erodynamic Particle Sizer to characterize

the aerodynamic particle size distribution of any aerosol present.

Samples were drawn for 20 seconds at 5 Lpm. The mass median

aerodynamic diameter, geometric standard deviation and total

concentration were calcul ated based on the amount of particle

collected.

See Table III for equipment details.

2.3.4. CHAMBER AND EXPOSURE ROOM ENVIRONMENT

Chamber oxygen levels (maintained at least 19%) were measured

pretest and at the beginning, middle, and end of the study.

Air sampl es were taken in the vapor generation area pretest and at

the beginning, middle and end of the study. Light (maintained

approximately 30 foot

- candles at 1.0 meters above the floor) and

noise levels (maintained below 85 decibels) in the exposure room

were measured pretest and at the beginning, middle and end of the

study.

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

# Huntingdon Life Sciences 00-4206 Page 134

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32

Group I

– 0 mg/m 3



## Normal

Within Normal Limi ts All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

# Huntingdon Life Sciences 00-4206 Page 135

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

# Huntingdon Life Sciences 00-4206 Page 136

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## No rmal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All A ll All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure D ay 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All A ll All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 m g/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Withi n Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112

Group I

– 0 mg/m 3



## Normal

With in Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All A ll All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All

- -

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All

-

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All

- -

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time. 

## Exposure Day 129 130

Group IV

– 20,00 0 mg/m 3



## Normal

Within Normal Limits All All

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Inhalation Report Appendix A

Ta ble II

Chamber Monitoring Results

Preface

Key to Abbreviations

MMAD = Mass Median Aerodynamic Diameter

GSD = Geometric Standard Deviation

TMC = Total Mass Concentration

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Table III

Equipment List

Exposure Chamber

1500 Liter glass and stainless steel chamber (Unifab Cages).

Chamber Air -flow Gauges

Dwyer ® 

# Magnehelic ® 

# gauge (Dwyer  Instruments Inc.), calibrated prestudy with a Side

Trak  III, equipped with a Digital Meter, Model 831- N2 (Sierra Instru ments, Inc.).

Chamber Static Pressure

Dwyer ® 

# Magnehelic ® 

# gauge (Dwyer ® 

# Instruments Inc.); calibrated prestudy with a

Dwyer ® 

# Mark II Manometer, Model 25 (Dwyer ® 

# Instruments Inc.)

Compound Generator

Counter- Current Volatilization Unit, coiled glass rod insert with nichrome wire

(Crown Glass Co., Inc.).

Flowmeter with built -in valve, size 0-65 mm, tube #’s 6G02R3, 6G03R3, 6G04R3

(Key Instruments).

Variable Autotransformers: Type 3PN 1010 (Staco Energy Products Company)

and Type PL 312, Minitrol (Glas

- col ® 

# Apparatus Company)

T ° Sentry Digital Alarm Module, Model 110 (Hampshire Controls Corp.).

Balston ® 

# Microfibre  Disposable Filter Units, No. L9933- 05 (Grade DQ).

Quick

- disconnect fitting with toggle valve (Rego  

# ).

Compound Reservoir

5-gallon cylinder ( Manchester Tank).

Balance

Mettler PM30000K (Mettler Instrument Corporation).

Pelouze, Model No. 4010.

Flowmeters

Flowmeter, size 0 -4, 0-5, 0-20, 0-30, 0-40 Lpm (Dwyer ® 

# Instruments Inc.).

Top Trak  Mass Flow Meter size 0 -1 Lpm, Model 821- 1 (Sierra Instrum ents),

calibrated prestudy with a Gilibrator  

# Bubble Generator, P/N D800286, S/N 569-S, flow

cell assembly P/N D80026B, BD #1860).

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Table III

Equipment List

Pressure/Vacuum Gauges

Ashcroft backpressure gauge, P/N 733-47

Matheson ® 

# backpressure gauge, P/N 63-3161.

Norgreen backpress ure gauge, P/N 9892K23.

Dwyer ® 

# Magnehelic ® 

# gauge (Dwyer ® 

# Instruments Inc.).

Gast ® 

# vacuum gauge, Model 169-01

Union Carbide backpressure gauge (P/N SG 8383)

Regulator

Norgreen (P/N 9892K23)

Valves

Metering Valve, Model SS

- 4L Series (Nupro ® 

# Co.).

Tubing

T eflon ® 

# , size 1/8”, 1/4”, 1/2”.

Plastic Size 1/4”, 1/2” (Norton, Baxter).

Stainless steel 1/2”.

T- Tube, stainless steel.

Glass elbow tube (Crown Glass Co.).

Stainless steel cross (Swage).

Filter

Balston ® 

# Microfibre  Disposable Filter Unit, No. L9933-05 (G rade DQ).

Air Analyzer

MIRAN ® 

# 1A- CVF Ambient Air Analyzer (Wilks) with a Cole Parmer strip recorder

Model 201 and a Micronta ® 

# LCD Benchtop Digital Multimeter No. 22 -195.

Oxygen/Gas Analyzer, Model 12145 (Gastech).

Digital Sound Level Meter, Model 840029 ( Speer Scientific).

Photometer/Light Meter (Quantum Instruments).

Particle Sizer/Analyzer

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Inhalation Report Appendix A

Table III

Equipment List

TSI Aerodynamic Particle Sizer, Model 331001 and a DELL computer, Model 486P/25,

equipped with an Epson LQ-570+ Dot matrix printer, Model P630B.

Absorbent Tube Sampl ing

Charcoal Tubes, ORBO-32, Lot #’s 15350 and 13843 (Supelco).

Vacuum Pumps

Thomas Industries Inc., Model 707CM50, 107CA183.

Timer

Gralab Universal Timer, Model 171.

Environmental Monitoring

VWR Big Digit Temperature and Humidity Gauge, tested prestudy with a VWR NIST

Traceable Digital Hygrometer/Thermometer.

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Inhalation Report Appendix A

Table IV

Chamber Distribution Records

Group

(target) Date Port IR Conc

(mg/m 3 

# ) Ratio to H

- 3

II (2,000 mg/m 3 

# ) 12

- August

- 02 H

- 3 1950 1.00

H

- 1 1420 0.73

H

- 2 1970 1.01

H

- 9 1700 0.87

H

- 3 1920 1.00

H

- 7 1360 0.71

H-8 1960 1.02

H

- 11 1410 0.73

H

- 3 2080 1.00

H

- 12 2160 1.03

H

- 13 1660 0.80

H

- 14 2210 1.06

H

- 3 2200 1.00

H

- 1 1660 0.75

H-7 1690 0.77

H

- 11 1680 0.76

II (2,000 mg/m 3 

# ) 14

- August

- 02 H

- 3 2260 1.00

H

- 1 1720 0.76

H

- 7 1580 0.70

H

- 11 1660 0.73

II (2,000 mg/m 3 

# ) 15

- August

- 02 H

- 3 1950 1.00

H-1 1920 0.98

H

- 2 1900 0.97

H

- 9 1900 0.97

H

- 7 1910 0.98

H

- 3 1950 1.00

H

- 8 1920 0.98

H

- 11 1930 0.99

H

- 12 1910 0.98

H-13 1940 0.99

H

- 14 1910 0.98

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Inhalation Report Appendix A

Table IV

Chamber Distribution Records

Group

(target) Date Port IR Conc

(mg/m 3 

# ) Ratio to H

- 3

III (10,000 mg/m 3 

# ) 13

- Au gust

- 02 H

- 3 10100 1.00

H

- 1 10000 0.99

H

- 2 9940 0.98

H

- 9 9780 0.97

H

- 3 9720 1.00

H

- 7 9750 1.00

H-8 9590 0.99

H

- 11 9560 0.98

H

- 3 9560 1.00

H

- 12 9490 0.99

H

- 13 9300 0.97

H

- 14 9270 0.97

IV (20,000 mg/m 3 

# ) 14

- August

- 02 H

- 3 19500 1.00

H-1 19300 0.99

H

- 2 21000 1.08

H

- 9 18800 0.96

H

- 3 19800 1.00

H

- 7 17900 0.90

H

- 8 20400 1.03

H

- 11 20900 1.06

H

- 3 20500 1.00

H

- 12 21600 1.05

H-13 21300 1.04

H

- 14 22100 1.08

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Table V

Miran Calibration

Methodology for Gasoline TBA Vapor Condensate

Settings: The instrument settings for the Miran 04 Unit are summarized below:

wavelength, microns 10.4

pathlength, dial setting 5.68

slit width, mm 1

range, absorbance 1A

response, seconds 1

gain Hig h

chart speed, cm/min 1

chart volts 1

Calibrations: The Miran ® 

# was turned on and allowed to warm up for approximately

10 minutes. The cell was flushed with room air for approximately one minute. The loop

was closed, the unit was zeroed and the calib ration series was performed as shown below.

The resultant data were plotted to obtain a calibration curve. Each observer used a

separate syringe for calibration.

Injection Calculated Absorbance

. 

# Volume Con centration 1 

# Operator 1 Operator 2 Average

(μL) (mg/m 3 

# ) (volts) (volts) (volts)

9 1053 0.0485 0.0344 0.0415

17 1989 0.0717 0.0652 0.0685

86 10064 0.331 0.325 0.328

170 19894 0.632 0.631 0.632

210 24575 0.748 0.770 0.759

1 

# Calcul ated Conc. (mg/m 3 

# ) = Injection volume (μL) x Density ( 0.66 mg/μL) x 1000 L/m 3



# 5.64 L (Volume of Miran cell)

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Table V

Miran Calibration

Calibration Checks: A three -point calibration check of the Miran ® 

# was performed for

each exposure prior to sampling the chambers. The parameters are shown below:

Expected Acceptable

Injection Calculated Absorbance Absorbance

Volume Concentration Reading Range

(μL) (mg/m 3 

# ) (volts) (volts)

17 1989 0.0685 0.0582 – 0.0788

86 10064 0.328 0.279 – 0.377

170 19894 0.632 0.537 – 0.727

The absorbance was recorded after each injection. The absorbance was considered

satisfactory if it was within 15% of the original calibration series. If any of the

absorbance values fell outside the 15% range, the injection was rechecked as follows.

The volume for the value that was out of range was reinjected twice. The closer pair of

the three injections were averaged and the results were compared to the original curve. If

the average of the pair wa s within the 15% range, the original was accepted. If the value

of the average was outside the 15% range, the Study Director decided if a new graph was

to be prepared.

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Table V

Miran Calibration

Calibration Curve for Gasoline TBA Vapor Condensate

(Groups I

– IV)

y = 22403x 4 

## - 33856x 3 

## + 19834x 2 

## + 26953x

R 2 

## = 0.9994

0 5000 10000 15000 20000 25000 30000

0.0000 0.2000 0.4000 0.6000 0.8000 1.0000

Absorbance (Volts)Concentration (mg/m3)

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Inhalation Report Appendix A

Table VI

Testing Room Environmental Monitoring

Interval Location Test

Substance

(mg/m 3 

# ) Light

(Ft Candles) Noise

(dB) Oxygen

(%) Particle

Sizing

(mg/m 3 

# ) a



# Pretest Room 809 0 33.4 60.5

- Group I Chamber

- - - 20 3.26 x 10 - 3



# Group II Chamber

- - - 20 1 .99 x 10 - 3



# Group III Chamber

- - - 20 3.51 x 10 - 3



# Group IV Chamber

- - - 20 2.52 x 10 - 3



# Week 0 Room 809 0 34.5 62.0

- Group I Chamber

- - - 20

Group II Chamber

- - - 20

Group III Chamber

- - - 20

Group IV Chamber

- - - 20

Week 9 Room 809 0 37.2 66.8

- Group I Chamber

- - - 20

Group II Chamber

- - - 20

Group III Chamber

- - - 20

Group IV Chamber

- - - 20

Week 17 Room 809 0 34.8 65.3

- Group I Chamber

- - - 19

Group II Chamber

- - - 20

Group III Chamber

- - - 20

Group IV Chamber

- - - 20

a 

# Pretest results presented below. For Weeks 0, 9 and 17 results, see Chamber Monitoring

Records (Table II).

# Huntingdon Life Sciences 00-4206 Page 173

211- TBA -1G Final Report

Group I Inhalation Report Appendix A

Figure I

Chamber Generation System

and Whole-Body Exposure Chamber

Notes:

1. Sampling Ports H

- 11 (left

- bottom), H

- 12 (left

- top), H

- 13 (right

- bottom) and H -14

(right

- top) used for pretest distribution sampling, were located on the back wall of the

chambers.

2. Animals were individually housed on five levels within the exp osure chamber.

# Huntingdon Life Sciences 00-4206 Page 174

211- TBA -1G Final Report

Groups II, III and

IV Inhalation Report Appendix A

Figure II

Chamber Generation System

and Whole-Body Exposure Chamber

Notes:

1. Sampling Ports H

- 11 (left

- bottom), H

- 12 (left

- top), H

- 13 (right

- bottom) and H -14

(right

- top) used for pretest distribution sampling, were located on the back wall of the

chambers.

2. Animals were individually housed on five levels within the exposure chamber.23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 206

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

1026 TERMINAL SACRIFICE 11

- DEC

- 02 113

1027 T ERMINAL SACRIFICE 11

- DEC

- 02 113

1028 TERMINAL SACRIFICE 11

- DEC

- 02 113

1029 TERMINAL SACRIFICE 11

- DEC

- 02 113

1030 TERMINAL SACRIFICE 11

- DEC

- 02 113

1031 TERMINAL SACRIFICE 11

- DEC

- 02 113

1032 TERMINAL SACRIFICE 11

- DEC

- 02 113

1033 TERMINAL SACRIFICE 11

- DEC

- 02 113

1034 TERMINAL SACRIFICE 11

- DEC

- 02 113

1035 TERMINAL SACRIFICE 11

- DEC

- 02 113

1036 TERMINAL SACRIFICE 11

- DEC

- 02 113

1037 TERMINAL SACRIFICE 11

- DEC

- 02 113

1038 TERMINAL SACRIFICE 11

- DEC

- 02 113

1039 TERMINAL SACRIFICE 12

- DEC

- 02 114

1040 TERMINAL SACRIFICE 12

- DEC

- 02 114

1041 TERMINAL SACRIFICE 12

- DEC

- 02 114

1042 TERMINAL SACRIFICE 12

- DEC

- 02 114

1043 TERMINAL SACRIFICE 12

- DEC

- 02 114

104 4 TERMINAL SACRIFICE 12

- DEC

- 02 114

1045 TERMINAL SACRIFICE 12

- DEC

- 02 114

1046 TERMINAL SACRIFICE 12

- DEC

- 02 114

1047 TERMINAL SACRIFIC E 12

- DEC

- 02 114

1048 TERMINAL SACRIFICE 12

- DEC

- 02 114

1049 TERMINAL SACRIFICE 12

- DEC

- 02 114

1050 TERMINAL SACRIFICE 12

- DEC

- 02 11 4

1051 TERMINAL SACRIFICE 12

- DEC

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 207

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

I NDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-------------------------------------------------------------------------------------------------------------------------------- ---

2026 TERMINAL SACRIFICE 11

- DEC

- 02 113

2027 TERMINAL SACRIFICE 11

- DEC

- 02 113

2028 TERMINAL SACRIFICE 11

- DEC

- 02 113

2029 TERMINAL SACRIFICE 11

- DEC

- 02 113

2030 TERMINAL SACRIFICE 11

- DEC

- 02 113

2031 TERMINAL SACRIFICE 11

- DEC

- 02 113

2032 TERMINAL SACRIFICE 11

- DEC

- 02 113

2033 TERMINAL SACRIFICE 11

- DEC

- 02 113

2034 TERMINAL SACR IFICE 11

- DEC

- 02 113

2035 TERMINAL SACRIFICE 11

- DEC

- 02 113

2036 TERMINAL SACRIFICE 11

- DEC

- 02 113

2037 TERMINAL SACRIFICE 11

- DEC

- 02 113

2038 TERMINAL SACRIFICE 11

- DEC

- 02 113

2039 TERMINAL SACRIFICE 12

- DEC

- 02 114

2040 TERMINAL SACRIFICE 12

- DEC

- 02 114

2041 TERMINAL SACRIFICE 12

- DEC

- 02 114

2042 TERMINAL SACRIFICE 12

- DEC

- 02 114

2043 TERMINAL SACRIFICE 12

- DEC

- 02 114

2044 TERMINAL SACRIFICE 12

- DEC

- 02 114

2045 TERMINAL SACRIFICE 12

- DEC

- 02 114

2046 TERMINAL SACRIFICE 12

- DEC

- 02 114

2047 TERMINAL SACRIFICE 12

- DEC

- 02 114

2048 TERMINAL SACRIFICE 12

- DEC

- 02 114

2049 TERMINAL SACRIFICE 12

- DEC

- 02 114

2050 TERMINAL SACRIFICE 12

- DEC

- 02 114

2051 T ERMINAL SACRIFICE 12

- DEC

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 208

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

3026 TERMINAL SACRIFICE 11

- DEC

- 02 113

3027 TERMINAL SACRIFICE 11

- DEC

- 02 113

302 8 TERMINAL SACRIFICE 11

- DEC

- 02 113

3029 TERMINAL SACRIFICE 11

- DEC

- 02 113

3030 TERMINAL SACRIFICE 11

- DEC

- 02 113

3031 TERMINAL SACRIFIC E 11

- DEC

- 02 113

3032 TERMINAL SACRIFICE 11

- DEC

- 02 113

3033 TERMINAL SACRIFICE 11

- DEC

- 02 113

3034 TERMINAL SACRIFICE 11

- DEC

- 02 11 3

3035 TERMINAL SACRIFICE 11

- DEC

- 02 113

3036 TERMINAL SACRIFICE 11

- DEC

- 02 113

3037 TERMINAL SACRIFICE 11

- DEC

- 02 113

3038 TERMINAL SACRIFICE 11

- DEC

- 02 113

3039 TERMINAL SACRIFICE 12

- DEC

- 02 114

3040 TERMINAL SACRIFICE 12

- DEC

- 02 114

3041 TERMINAL SACRIFICE 12

- DEC

- 02 114

3042 TERMINAL SACRIFICE 12

- DEC

- 02 114

3043 TERMINAL SACRIFICE 12

- DEC

- 02 114

3044 TERMINAL SACRIFICE 12

- DEC

- 02 114

3045 TERMINAL SACRIFICE 12

- DEC

- 02 114

3046 TERMINAL SACRIFICE 12

- DEC

- 02 114

3047 TERMINAL SACRIFICE 12

- DEC

- 02 114

3048 TERMI NAL SACRIFICE 12

- DEC

- 02 114

3049 TERMINAL SACRIFICE 12

- DEC

- 02 114

3050 TERMINAL SACRIFICE 12

- DEC

- 02 114

3051 TERMINAL SACRIFICE 12

- DEC

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 209

APPENDIX C

GASOLINE TBA VAP OR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

4026 TERMINAL SACRIFICE 11

- DEC

- 02 113

4027 TERMINAL SACRIFICE 11

- DEC

- 02 113

4028 TERMINAL SACRIFICE 11

- DEC

- 02 113

4029 TERMINAL SACRIFICE 11

- DEC

- 02 113

4030 TERMINAL SACRIFICE 11

- DEC

- 02 113

4031 TERMINAL SACRIFICE 11

- DEC

- 02 113

4032 TERMINAL SACRIFICE 11

- DEC

- 02 113

4033 TERMINAL SACRIFICE 11

- DEC

- 02 113

4034 TERMINAL SACRIFICE 11

- DEC

- 02 113

4035 T ERMINAL SACRIFICE 11

- DEC

- 02 113

4052 TERMINAL SACRIFICE 11

- DEC

- 02 113

4037 TERMINAL SACRIFICE 11

- DEC

- 02 113

4038 TERMINAL SACRIFICE 11

- DEC

- 02 113

4039 TERMINAL SACRIFICE 12

- DEC

- 02 114

4040 TERMINAL SACRIFICE 12

- DEC

- 02 114

4041 TERMINAL SACRIFICE 12

- DEC

- 02 114

4042 TERMINAL SACRIFICE 12

- DEC

- 02 114

4043 TERMINAL SACRIFICE 12

- DEC

- 02 114

4044 TERMINAL SACRIFICE 12

- DEC

- 02 114

4045 TERMINAL SACRIFICE 12

- DEC

- 02 114

4046 TERMINAL SACRIFICE 12

- DEC

- 02 114

4047 TERMINAL SACRIFICE 12

- DEC

- 02 114

4048 TERMINAL SACRIFICE 12

- DEC

- 02 114

4049 TERMINAL SACRIFICE 12

- DEC

- 02 114

4050 TERMINAL SACRIFICE 12

- DEC

- 02 114

4051 TERMINAL SACRIFICE 12

- DEC

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 210

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GR OUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

1526 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

1527 DPP FOUND DEAD 24

- NOV

- 02 96 P

1528 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

1529 PNMD TERMINAL SACRIFICE 25

- DEC

- 02 127 P

1530 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

1531 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

1532 TERMI NAL SACRIFICE 26

- NOV

- 02 98 NP

1533 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

1534 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

1535 TERMINAL SACRIFICE 24

- NOV

- 02 96 NP

1536 TERMINAL SACRIFICE 23

- DEC

- 02 125 P

1537 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

1538 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1539 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1540 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1541 TERMINAL SACRIFICE 25

- NOV

- 02 97 NP

1542 TERMINAL SACR IFICE 19

- DEC

- 02 121 P

1543 TERMINAL SACRIFICE 24

- DEC

- 02 126 P

1544 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

1545 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1546 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1547 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1548 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

1549 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

1550 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

1551 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 211

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP II 2000 MG/M3

---------------------------------------------------------------------------------------------------------------------------------- -

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

2526 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

2527 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

2528 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

2529 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

2530 TERMINAL SACRIFICE 26

- DEC

- 02 128 P

2531 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

2532 TERMINAL SACRIFICE 25

- NOV

- 02 97 NP

2533 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

2534 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

2535 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

2536 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

2537 TERMINA L SACRIFICE 18

- DEC

- 02 120 P

2538 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

2539 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

2540 TERMINAL SACRIFICE 21

- DEC -0 2 123 P

2541 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

2542 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

2543 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

2 544 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

2545 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

2546 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

2547 TERMINAL SACRIF ICE 22

- DEC

- 02 124 P

2548 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

2549 TLL TERMINAL SACRIFICE 17

- DEC

- 02 119 P

2550 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

2551 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

NNM D=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 212

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERAT ION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

3526 TERMINAL SACRIFICE 24

- NOV

- 02 96 NP

3527 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

3528 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

3529 TERMINAL SACRIFICE 17

- DEC

- 02 119 P

3530 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3531 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3532 T ERMINAL SACRIFICE 19

- DEC

- 02 121 P

3533 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

3534 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3535 TERMINAL SACRIFICE 25

- DEC

- 02 127 P

3536 TLL TERMINAL SACRIFICE 17

- DEC

- 02 119 P

3537 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3538 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

3539 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

3540 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3541 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

3542 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3543 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3544 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

3545 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

3546 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

3547 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

3548 TLL TERMINAL SACRIFICE 17

- DEC

- 02 119 P

354 9 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

3550 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

3551 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS23

- JUN

- 2009 17:31

Huntingdon Life Sciences 00

- 4206 PAGE 213

APPENDIX C

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP IV 20000 MG/M3

- ----------------------------------------------------------------------------------------------------------------------------- -----

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

4526 TERMINAL SACRIFICE 25

- DEC

- 02 127 P

4527 TERMINAL SACRIFICE 21

- DEC

- 02 123 P

4528 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

4529 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

4530 TERMINAL SACRIFICE 27

- NOV

- 02 99 NP

4531 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

4532 TERMINAL SACRIFICE 23

- DEC

- 02 125 P

4533 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

4534 TERMINAL SACRIFICE 22

- DEC

- 02 124 P

4535 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

4536 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

4537 TER MINAL SACRIFICE 24

- DEC

- 02 126 P

4538 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

4539 TERMINAL SACRIFICE 24

- NOV

- 02 96 NP

4540 TERMINAL SACRIFICE 18 -D EC

- 02 120 P

4541 NNMD TERMINAL SACRIFICE 7

- DEC

- 02 109 NP

4542 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

4543 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

4544 TERMINAL SACRIFICE 18

- DEC

- 02 120 P

4545 TERMINAL SACRIFICE 26

- DEC

- 02 128 P

4546 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

4547 PNMD TERMINAL SA CRIFICE 21

- DEC

- 02 123 P

4548 TERMINAL SACRIFICE 20

- DEC

- 02 122 P

4549 DIED FOUND DEAD 28

- SEP

- 02 39

-

4550 TERMINAL SACRIFICE 29

- DEC

- 02 131 P

4551 TERMINAL SACRIFICE 19

- DEC

- 02 121 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

DIED=FOUND DEAD PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

# Huntingdon Life Sciences 00-4206 Page 214

211-TBA-1G Final Report

Individual Clinical Observations

(P0 Generation)

Preface Appendix D

For summarization purposes, descriptive com ment s [i.e., location of scab(s) and sore(s),

etc.] are not presented in this appendix. Thes e data are contained in the study raw data if

needed.

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m 3



# Group II - 2,000 mg/m 3



# Group III - 10,000 mg/m 3



# Group IV - 20,000 mg/m 3



# Note:

If an anim al died am id two scheduled obser vation days, the death is presented at the

scheduled observation day prior to the death (for example, if an anim al died on Study

Day 39, its’ death is presented as Study Day 35). Term inal Sacrifices for m ales are

presented as Study Day 112 rather than St udy Day 119, and term inal sacrifices for

unmated females are presented as Study Day 105 rather than Study Day 112, due to

computer limitations.24-JUN-2009 11:35Huntingdon Life Sciences 00-4206 PAGE 215APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------1026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1027 WITHIN NORMAL LIMITS P P P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED P P P P P P P P P P P1028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P PTERMINAL SACRIFICE PINCISORS BROKEN/MISSING P P P P1030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:35Huntingdon Life Sciences 00-4206 PAGE 216APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------1035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1040 WITHIN NORMAL LIMITS P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 3ALOPECIA - GENERAL 31041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1042 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:35Huntingdon Life Sciences 00-4206 PAGE 217APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------1044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1046 WITHIN NORMAL LIMITS P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 3 3 3 2 2 2 21047 WITHIN NORMAL LIMITS P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 3 3 3 3 3CHROMODACRYORRHEA - BILATERAL PINCISORS MALOCCLUDED P1048 WITHIN NORMAL LIMITS P P P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED P P P P P P P P P P PINCISORS BROKEN/MISSING P P P P P P P PORAL SORE P1049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 218APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------2026 WITHIN NORMAL LIMITS P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2ULCERATION - CERVICAL 22027 WITHIN NORMAL LIMITS P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 3 3 3 32028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P PTERMINAL SACRIFICE PULCERATION - CERVICAL P P PALOPECIA - GENERAL 22030 WITHIN NORMAL LIMITS P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 22031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P PTERMINAL SACRIFICE PINCISORS BROKEN/MISSING P P P2033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 219APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------2034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2038 WITHIN NORMAL LIMITS P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 3 32039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - GENERAL 22040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2041 WITHIN NORMAL LIMITS P P P P P P P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED P P P P P P PINCISORS BROKEN/MISSING P P P P2042 WITHIN NORMAL LIMITS P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED P P P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 220APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------2043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED PINCISORS BROKEN/MISSING P2044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2048 WITHIN NORMAL LIMITS P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED P P P P P P P P P P P P PINCISORS BROKEN/MISSING P P P P2049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2050 WITHIN NORMAL LIMITS P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2INCISORS MALOCCLUDED P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 221APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------2051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 222APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------3026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3029 WITHIN NORMAL LIMITS P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3BLACK/BROWN STAINS ON SNOUT 13030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 223APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------3035 WITHIN NORMAL LIMITS P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 2 23036 WITHIN NORMAL LIMITS P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 2 2 2 2 2 23037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3038 WITHIN NORMAL LIMITS P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 2 2 2 2 23039 WITHIN NORMAL LIMITS P P P P P P P PTERMINAL SACRIFICE PCHROMODACRYORRHEA - BILATERAL PCHROMODACRYORRHEA - UNILATERAL PINCISORS MALOCCLUDED P PINCISORS BROKEN/MISSING P P P P P P P P3040 WITHIN NORMAL LIMITS P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 3 3 3 3 3 3 2 2 2 23041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 224APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------3042 WITHIN NORMAL LIMITS P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 2 2 23043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3044 WITHIN NORMAL LIMITS P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 3 3 3 3 3 3ALOPECIA - GENERAL 3 3 33045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3046 WITHIN NORMAL LIMITS P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3 3 3 3 2 2 2 2 2 2INCISORS BROKEN/MISSING P P3047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 225APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------3050 WITHIN NORMAL LIMITS P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 3 3 3 3 23051 WITHIN NORMAL LIMITS P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 2 2 2 2 2 2 2 2 2-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 226APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------4026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4027 WITHIN NORMAL LIMITS P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 24028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4029 WITHIN NORMAL LIMITS P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 2INCISORS MALOCCLUDED PINCISORS BROKEN/MISSING P P4030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 24033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 227APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------4034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4052 WITHIN NORMAL LIMITS P P P P P P P P PTERMINAL SACRIFICE PINCISORS MALOCCLUDED P P P P P P P P P4037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4038 WITHIN NORMAL LIMITS P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 34039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4040 WITHIN NORMAL LIMITS P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 3 3 3 24041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4042 WITHIN NORMAL LIMITS P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 2 2 2 2 2-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:34Huntingdon Life Sciences 00-4206 PAGE 228APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1 1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2-----------------------------------------------------------------------------------------------------------------------------------4043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 24044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4047 WITHIN NORMAL LIMITS P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 2 2 24048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 229APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------1526 WITHIN NORMAL LIMITS P P P P P P P P P P P1527 WITHIN NORMAL LIMITS P P P P P P P P P P P P1528 WITHIN NORMAL LIMITS P P P P P P P P P P P P1529 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P1530 WITHIN NORMAL LIMITS P P P P P P P P P P P P1531 WITHIN NORMAL LIMITS P P P P P P P P P P P1532 WITHIN NORMAL LIMITS P P P P P P P P P P P P1533 WITHIN NORMAL LIMITS P P P P P P P P P P P1534 WITHIN NORMAL LIMITS P P P P P P P P P P P1535 WITHIN NORMAL LIMITS P P P P P P P P P P P1536 WITHIN NORMAL LIMITS P P P P P P P P P P P P1537 WITHIN NORMAL LIMITS P P P P P P P P PINCISORS MALOCCLUDED P P P1538 WITHIN NORMAL LIMITS P P P P P P P P P P P P1539 WITHIN NORMAL LIMITS P P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 230APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------1540 WITHIN NORMAL LIMITS P P P P P P P P P P P P1541 WITHIN NORMAL LIMITS P P P P P P P P P P P P1542 WITHIN NORMAL LIMITS P P P P P P P P P P P P1543 WITHIN NORMAL LIMITS P P P P P P P P P P P P1544 WITHIN NORMAL LIMITS P P P P P P P P P P P P1545 WITHIN NORMAL LIMITS P P P P P P P P P P P P1546 WITHIN NORMAL LIMITS P P P P P P P P P P P P1547 WITHIN NORMAL LIMITS P P P P P P P P P P P P1548 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1549 WITHIN NORMAL LIMITS P P P P P P P P P P P P1550 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P1551 WITHIN NORMAL LIMITS P P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 231APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------2526 WITHIN NORMAL LIMITS P P P P P PINCISORS MALOCCLUDED P P P P P P2527 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2528 WITHIN NORMAL LIMITS P P P P P P P P P P P P2529 WITHIN NORMAL LIMITS P P P P P P P P P P P P2530 WITHIN NORMAL LIMITS P P P P P P P P P P P P P2531 WITHIN NORMAL LIMITS P P P P P P P P P P P P2532 WITHIN NORMAL LIMITS P P P P P P P P P P P P2533 WITHIN NORMAL LIMITS P P P P P P P P P P P P2534 WITHIN NORMAL LIMITS P P P P P P P P P P P P2535 WITHIN NORMAL LIMITS P P P P P P P P P P P2536 WITHIN NORMAL LIMITS P P P P P P P P P P P P2537 WITHIN NORMAL LIMITS P P P P P P P P P P P2538 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 232APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------2539 WITHIN NORMAL LIMITS P P P P P P P P P P P P2540 WITHIN NORMAL LIMITS P P P P P P PCHROMODACRYORRHEA - UNILATERAL P PINCISORS MALOCCLUDED P P P P P2541 WITHIN NORMAL LIMITS P P P P P P P P P P P P2542 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P2543 WITHIN NORMAL LIMITS P P P P P P P P P P P P2544 WITHIN NORMAL LIMITS P P P P P P P P P P P P2545 WITHIN NORMAL LIMITS P P P P P P P P P P P P2546 WITHIN NORMAL LIMITS P P P P P P P P P P P P2547 WITHIN NORMAL LIMITS P P P P P P P P P P P P2548 WITHIN NORMAL LIMITS P P P P P P P P P P P P2549 WITHIN NORMAL LIMITS P P P P P P P P P P P P2550 WITHIN NORMAL LIMITS P P P P P P P P P P P2551 WITHIN NORMAL LIMITS P P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 233APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------3526 WITHIN NORMAL LIMITS P P P P P P P P P P P P3527 WITHIN NORMAL LIMITS P P P P P P P P P P P3528 WITHIN NORMAL LIMITS P P P P P P P P P P P P3529 WITHIN NORMAL LIMITS P P P P P P P P P P P3530 WITHIN NORMAL LIMITS P P P P P P P P P P P P3531 WITHIN NORMAL LIMITS P P P P P P P P P P P P3532 WITHIN NORMAL LIMITS P P P P P P P P P P P P3533 WITHIN NORMAL LIMITS P P P P P PINCISORS MALOCCLUDED P P P P P P3534 WITHIN NORMAL LIMITS P P P P P P P P P P P P3535 WITHIN NORMAL LIMITS P P P P P P P P P P P P3536 WITHIN NORMAL LIMITS P P P P P P P PALOPECIA - EXTREMITIES/SNOUT 2 2 2 23537 WITHIN NORMAL LIMITS P P P P P P P P P P P P3538 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 234APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------3539 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P3540 WITHIN NORMAL LIMITS P P P P P P P P P P P P3541 WITHIN NORMAL LIMITS P P P P P P P P P P P3542 WITHIN NORMAL LIMITS P P P P P P P P P P PCHROMODACRYORRHEA - UNILATERAL P3543 WITHIN NORMAL LIMITS P P P P P P P P P P P P3544 WITHIN NORMAL LIMITS P P P P P P P P P P P P3545 WITHIN NORMAL LIMITS P P P P P P P P P P P P3546 WITHIN NORMAL LIMITS P P P P P P P P P P P P3547 WITHIN NORMAL LIMITS P P P P P P P P P P P P3548 WITHIN NORMAL LIMITS P P P P P P P P P P P P3549 WITHIN NORMAL LIMITS P P P P P P P P P P P P3550 WITHIN NORMAL LIMITS P P P P P P P P PTERMINAL SACRIFICE PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2ALOPECIA - GENERAL 2 2 2-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 235APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------3551 WITHIN NORMAL LIMITS P P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 236APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------4526 WITHIN NORMAL LIMITS P P P P P P P P P P P P4527 WITHIN NORMAL LIMITS P P P P P P P PALOPECIA - EXTREMITIES/SNOUT 2 2 2 24528 WITHIN NORMAL LIMITS P P P P P P P P P P P P4529 WITHIN NORMAL LIMITS P P P P P P P P P P P P4530 WITHIN NORMAL LIMITS P P P P P P P P P P P P4531 WITHIN NORMAL LIMITS P P P P P P P P P P P P4532 WITHIN NORMAL LIMITS P P P P P P P P P P P P4533 WITHIN NORMAL LIMITS P P P P P P P P P P P4534 WITHIN NORMAL LIMITS P P P P P P P P P P P P4535 WITHIN NORMAL LIMITS P P P P P P P P P PCHROMODACRYORRHEA - UNILATERAL P4536 WITHIN NORMAL LIMITS P P P P P P P P P P P P4537 WITHIN NORMAL LIMITS P P P P P P P P P P P P4538 WITHIN NORMAL LIMITS P P P P P PINCISORS MALOCCLUDED P P P P P PINCISORS BROKEN/MISSING P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 237APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------4539 WITHIN NORMAL LIMITS P P P P P P P P P P P P4540 WITHIN NORMAL LIMITS P P P P P P P P P P P4541 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P PTERMINAL SACRIFICE P4542 WITHIN NORMAL LIMITS P P P P P P P P P P P P4543 WITHIN NORMAL LIMITS P P P P P P P PALOPECIA - EXTREMITIES/SNOUT 2 2 2 2INCISORS MALOCCLUDED P PINCISORS BROKEN/MISSING P P4544 WITHIN NORMAL LIMITS P P P P P P P P P P P4545 WITHIN NORMAL LIMITS P P P P P P P P P P PYELLOW ANO-GENITAL STAINING 14546 WITHIN NORMAL LIMITS P P P P P P P P P P P P4547 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P4548 WITHIN NORMAL LIMITS P P P P P P P P P P P P4549 WITHIN NORMAL LIMITS P P P P P P PFOUND DEAD P4550 WITHIN NORMAL LIMITS P P P P P P P P P P P P P-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT24-JUN-2009 11:36Huntingdon Life Sciences 00-4206 PAGE 238APPENDIX DGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL WEEKLY CLINICAL OBSERVATIONSFEMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------1DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5-----------------------------------------------------------------------------------------------------------------------------------4551 WITHIN NORMAL LIMITS P P P P P P P P P PYELLOW ANO-GENITAL STAINING 1 1-----------------------------------------------------------------------------------------------------------------------------------CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT23

- JUN

- 2009 18:00

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APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEM ALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

1526 WITHIN NORMAL LIMITS P P P P

1527 WITHIN NORMAL LIMITS P P P P

1528 WITHIN NORMAL LIMITS P P P P

1530 WITHIN NORMAL LIMITS P P P P

1531 WITHIN NORMAL LIMITS P P P P

1532 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

1533 WITHIN NORMAL LIMITS P P P P

1534 WITHIN NORMAL LIMITS P P P P

1535 WITHIN NORMAL LIMITS P P P P

1536 WITHIN NORMAL LIMITS P P P P

1537 INCISORS MALOCCLUDED P P P

WITHIN NORMAL LIMITS P

1538 WITHIN NORMAL LIMITS P P P P

1539 WITHIN NORMAL LIMITS P P P P

1540 WITHIN NORMAL LIMITS P P P P

1541 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P -P RESENT23

- JUN

- 2009 18:00

Huntingdon Life Sciences 00

- 4206 PAGE 240

APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

---------------------------------------------------------------------------------------------------------------------------- -------

1542 WITHIN NORMAL LIMITS P P P P

1543 WITHIN NORMAL LIMITS P P P P

1544 WITHIN NORMAL LIMITS P P P P

1545 WITHIN NORMAL LIMITS P P P P

1546 WITHIN NORMAL LIMITS P P P P

1547 WITHIN NORMAL LIMITS P P P P

1549 WITHIN NORMAL LIMITS P P P P

1551 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:00

Hunting don Life Sciences 00

- 4206 PAGE 241

APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

IND IVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

2526 INCISORS BROKE N/MISSING P P P P

2528 WITHIN NORMAL LIMITS P P P P

2529 WITH IN NORMAL LIMITS P P P P

2530 WITHIN NORMAL LIMITS P P P P

2531 WITHIN NORMAL LIMITS P P P P

2532 TERMINAL SACRIFICE P

ALOPECIA - EXTREMITIES/SNOUT 3 3

WITHIN NORMAL LIMITS P P

2533 WITHIN NORMAL LIMITS P P P P

2534 WITHIN NORMAL LIMITS P P P P

2535 WITHIN NORMAL LIMITS P P P P

2536 WITHIN NORMAL LIMITS P P P P

2537 WITHIN NORMAL LIMITS P P P P

2539 INCISORS MALOCCLUDED P P

WITHIN NORMAL LIMITS P P

2540 CHROMODACRYORRHEA

- UNILATERAL P P P P

INCISORS MALOCCLUDED P P P P

2541 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:00

Huntingdon Life Sciences 00

- 4206 PAGE 242

APPE NDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIO NS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

2543 WITHIN NORMAL LIMITS P P P P

2544 WITHIN NORMAL LIMITS P P P P

2545 WITHIN NORMAL LIMITS P P P P

2546 WITHIN NORMAL LIMITS P P P P

2547 WITHIN NORMAL LIMITS P P P P

2548 WITHIN NORMAL LIMITS P P P P

2549 WITHIN NORMAL LIMITS P P P P

2550 WITHIN NORMAL LIMITS P P P P

2551 CHROMODACRYORRHEA

- UNILATERAL P

INCISORS MALOCCLUDED P

WITHIN NORMAL LIMITS P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:00

Huntingdon Life Sciences 00

- 4206 PAGE 243

APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

3526 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

3527 INCISORS BROKEN/MISSING P

WITHIN NORMAL LIMITS P P P

3528 WITHIN NORMAL LIMITS P P P P

3529 WITHIN N ORMAL LIMITS P P P P

3530 WITHIN NORMAL LIMITS P P P P

3531 WITHIN NORMAL LIMITS P P P P

3532 WITHIN NORMAL LIMITS P P P P

3533 INCISORS MALOCCLUDED P P P P

3534 WITHIN NORMAL LIMITS P P P P

3535 WITHIN NORMAL LIMITS P P P P

3536 ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

3537 WITHIN NORMAL LIMITS P P P P

3540 CHROMODACRYORRHEA

- UNILATERAL P

INCISORS MALOCCLUDED P

WITHIN NORMAL LIMITS P P P

3541 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:00

Huntingdon Life Sciences 00

- 4206 PAGE 244

APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

3542 INCISORS BROKEN/MISSING P

WITHIN NORMAL LIMITS P P P

3543 INCISORS BROKEN/MISSING P

WITHIN NORMAL LIMITS P P P

3544 WITHIN NORMAL LIMITS P P P P

3545 WITHIN NORMAL LIMITS P P P P

3546 WITHIN NORMAL LIMITS P P P P

3 547 WITHIN NORMAL LIMITS P P P P

3548 WITHIN NORMAL LIMITS P P P P

3549 WITHIN NORMAL LIMITS P P P P

3551 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:00

Huntingdon Life Sciences 00

- 4206 PAGE 245

APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURI NG GESTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

4526 WITHIN NORMAL LIMITS P P P P

4527 ALOPECIA

- EXTREMITIES/SNOUT 2 2

WITHIN NORMAL LIMITS P P

4528 WITHIN NORMAL LIMITS P P P P

4529 WITHIN NORMAL LIMITS P P P P

4530 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

4531 WITHIN NORMAL LIMITS P P P P

4532 WITHIN NOR MAL LIMITS P P P P

4533 WITHIN NORMAL LIMITS P P P P

4534 WITHIN NORMAL LIMITS P P P P

4535 WITHIN NORMAL LIMITS P P P P

4 536 WITHIN NORMAL LIMITS P P P P

4537 WITHIN NORMAL LIMITS P P P P

4538 WITHIN NORMAL LIMITS P P P P

4539 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

4540 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:00

Huntingdon Life Sciences 00

- 420 6 PAGE 246

APPENDIX E

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVAT IONS DURING GESTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTA TION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

4542 WITHIN NORMAL LIMITS P P P P

4543 ALOPECIA

- EXTREMITIES/SNOUT 2

INCISORS MALOCCLUDED P P P P

INCISORS BROKEN/MISSING P P P P

4544 WITHIN NORMAL LIMITS P P P P

4545 WITHIN NORMAL LIMITS P P P P

4546 WITHIN NORM AL LIMITS P P P P

4548 WITHIN NORMAL LIMITS P P P P

4550 W ITHIN NORMAL LIMITS P P P P

4551 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 247

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1526 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1527 FOUND DEAD P

1528 WITHIN NORMAL LIMITS P P P P P

TERMINAL SACRIFICE P

CHROMODACRYORRHEA - UNILATERAL P

INCISORS MALOCCLUDED P

1529 WITHIN NORMAL LIMITS a P P P P P

TERMINAL SACRIFICE P

1530 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1533 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1534 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1536 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1537 TERMINAL SACRIFICE P

INCISORS MALOCCLUDED P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT

a Observations were not performed on Lactation Day 1 due to technician error.23

- JUN

- 2009 18:01

Huntingdon Life Sciences 0 0- 4206 PAGE 248

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1538 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1539 WITHIN NORMAL LIM ITS P P P P P P

TERMINAL SACRIFICE P

1540 WITHIN NOR MAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1542 WIT HIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1543 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

154 4 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1546 TERMINAL SACRIFICE P

INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P P

1547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1549 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 249

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1551 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MA RKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 250

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2526 TERMINAL SACRIFICE P

INCISORS BROKEN/MISSING P P P P P P

2528 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2529 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2530 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2533 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2534 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2535 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2536 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MAR KED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 251

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2539 TERMINAL SACRIFICE P

INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P

2540 TERMINAL SACRIFICE P

CHROMODACRYORRHEA - UNILATERAL P P P

INCISORS MALOCCLUDED P P P P P P

2541 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2543 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2544 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2546 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2548 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2549 WITHIN NORMAL LIMITS P P P P P

TERMINAL SACRIF ICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3- MARKED P

- PRESENT23

- JUN

- 2009 18:01

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- 4206 PAGE 252

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2550 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2551 TERMINAL SACRIFICE P

CHROMODACRYORRHEA - UNILATERAL P P P P P

INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingd on Life Sciences 00

- 4206 PAGE 253

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUA L MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3527 WITHI N NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3528 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3529 TERMINAL SACRIFICE P

EARS SWOLLEN/RED

- BILATERAL P P P P P P

3530 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3532 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3533 TERMINAL SACRIFICE P

INCISORS MALOCCLUDED P P P P P P

3534 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3535 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3536 TERMINAL SACRIFICE P

ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2

INCISORS BROKEN/MISSING P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 254

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3540 TERMINAL SACRIFICE P

CHROMODACRYORRHEA - UNILATERAL P P P P P P

INCISORS MALOCCLUDED P P P P P P

3541 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3542 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3543 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3544 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3546 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3548 WITHIN NORMAL LIMITS P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Hunt ingdon Life Sciences 00

- 4206 PAGE 255

APPENDIX F

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIV IDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVAT IONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3549 W ITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3551 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 256

APPENDIX F

GASO LINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4526 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4527 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4528 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4529 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4532 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4533 WITHIN NORMAL LIM ITS P P P P P P

TERMINAL SACRIFICE P

4534 WITHIN NOR MAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4535 WIT HIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4536 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 257

APPENDIX F

GASOLI NE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4538 WITHIN NORMAL LIMITS P P

TERMINAL SACRIFICE P

INCISORS BROKEN/MISSING P P P P

4540 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4542 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4543 TERMINAL SACRIFICE P

INCISORS MALOCCLUDED P P P P P P

INCISORS BROKEN/MISSING P P P P P P

4544 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4546 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4548 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:01

Huntingdon Life Sciences 00

- 4206 PAGE 258

APPENDIX F

GA SOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4550 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4551 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 18:04

Huntingdon Life Sciences 00

- 4206 PAGE 259

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

1026 135 194 250 300 340 368 391 414 434 456 472 489 494 507 511 516

1027 107 169 226 279 325 352 378 393 415 428 454 464 470 483 496 500

1028 117 189 242 293 332 361 383 405 429 438 443 461 476 491 515 516

1029 121 193 251 297 348 377 413 439 465 475 484 501 504 518 521 529

1030 121 190 250 304 358 386 416 449 474 493 516 533 545 565 575 584

1031 105 168 222 282 332 358 385 415 439 451 468 477 488 496 497 503

1032 124 190 239 290 323 346 369 390 418 428 444 469 477 492 499 502

1033 122 203 269 345 412 455 506 537 574 596 614 642 647 674 668 693

1034 110 179 237 300 358 393 439 460 481 501 527 539 564 578 587 603

1035 128 198 245 305 348 373 405 424 438 441 461 479 489 504 515 551

1036 112 179 228 284 339 37 1 411 430 450 465 490 488 496 512 518 526

1037 115 187 243 310 362 397 426 450 481 490 504 518 518 528 541 568

1038 117 191 256 323 3 83 409 438 466 496 499 509 517 540 554 563 581

1039 108 178 228 290 335 356 392 417 439 453 470 484 502 514 526 541

1040 114 186 250 319 379 420 449 473 500 518 535 550 574 588 601 624

1041 104 169 221 276 318 355 389 410 431 451 462 484 505 512 531 549

1042 125 195 251 303 352 384 424 446 476 491 506 521 537 548 564 574

1043 130 194 252 299 344 374 411 428 456 464 477 495 505 519 535 544

1044 112 186 243 295 347 372 410 430 443 450 466 478 488 501 514 531

1045 109 174 219 268 303 313 332 343 360 375 384 385 396 408 423 433

1046 108 173 225 280 333 365 395 418 443 458 478 486 505 518 526 529

1047 113 181 244 316 372 405 437 467 484 502 528 535 538 556 568 576

104 8 104 170 223 282 326 349 372 388 401 418 434 434 436 436 445 458

1049 105 169 217 283 334 369 398 426 447 444 457 470 492 508 525 538

1050 115 187 254 312 360 391 417 444 468 480 494 510 522 524 539 550

1051 133 209 273 338 388 411 442 466 484 503 517 535 543 559 574 583

MEAN 116 185 241 299 348 377 409 432 455 468 484 498 510 523 534 54 6

S.D. 9.1 11.2 15.2 18.8 24.4 28.5 33.2 36.6 39.8 41.4 43.1 46.2 47.1 50.1 48.2 51.6

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:04

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- 4206 PAGE 260

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GE NERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MA LES GROUP II 2000 MG/M3

----------------------------------------------------------------------------------------------------------------------------- - -----

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

2026 131 200 259 314 358 404 439 470 489 506 525 525 530 548 562 574

2027 112 175 223 281 335 372 410 438 466 475 492 509 525 535 546 552

2028 118 189 246 309 356 397 432 451 484 504 521 530 532 545 555 566

2029 109 179 236 297 349 391 436 471 495 517 544 556 579 592 610 618

2030 127 201 255 317 366 407 439 468 497 515 538 556 562 567 585 596

2031 125 195 251 310 355 389 420 443 468 481 498 507 515 527 539 555

2032 120 190 246 298 346 379 400 436 457 482 500 512 536 552 568 576

2033 125 190 255 321 365 400 437 473 492 512 530 551 567 589 599 619

2034 112 171 224 283 328 351 391 416 434 455 473 490 500 512 525 533

2035 105 171 226 285 330 364 399 421 439 454 472 481 492 506 510 521

2036 132 209 268 339 384 416 441 463 479 496 517 536 538 554 560 574

2037 109 174 234 290 340 363 397 421 449 464 482 493 506 522 529 529

2038 108 173 229 279 317 350 369 399 415 429 454 461 478 497 507 515

2039 121 192 253 314 363 401 434 465 480 506 516 528 533 561 575 582

2040 106 168 215 261 302 337 367 387 413 430 447 459 455 484 499 516

2041 135 2 04 262 315 364 400 440 457 477 495 514 512 531 535 545 564

2042 115 186 253 318 381 423 460 490 519 534 551 575 581 592 606 619

2043 106 172 223 279 334 361 396 419 443 452 458 469 484 501 516 526

2044 104 168 219 267 303 317 344 364 381 400 420 429 446 457 469 477

2045 122 190 249 305 353 381 413 422 446 455 472 481 486 490 506 517

2046 116 189 247 313 368 399 430 455 471 484 498 513 524 531 538 557

2047 114 183 240 300 340 368 411 430 456 472 491 498 513 517 523 532

2048 117 187 239 297 338 368 396 406 425 432 445 451 467 472 473 487

2049 114 176 229 277 325 357 388 411 440 448 461 480 488 498 500 519

2050 103 160 218 282 322 361 392 412 428 442 470 482 505 518 52 3 529

2051 111 177 231 291 327 358 389 414 431 445 464 490 495 524 535 541

MEAN 116 183 240 298 344 377 410 435 457 472 491 503 514 528 539 550

S.D. 9.1 12.6 15.1 18.8 21.8 25.7 27.8 30.4 31.7 33.6 34.1 35.6 35.4 35.7 37.6 38. 1

N 26 26 26 26 26 26 26 26 26 26 26 26 26 2 6 26 2623

- JUN

- 2009 18:04

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- 4206 PAGE 261

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

3026 104 165 209 255 286 314 333 347 366 383 389 401 419 429 436 447

3027 115 182 237 295 339 358 395 420 428 446 460 474 494 512 507 512

3028 117 186 240 307 353 385 420 444 470 496 524 544 562 569 571 591

3029 118 185 250 304 355 384 416 434 457 480 492 502 517 533 540 541

3030 104 166 223 280 331 370 406 443 468 486 510 517 545 560 575 581

3031 118 187 237 280 319 343 373 393 410 428 441 453 464 473 481 475

3032 106 171 215 269 310 336 359 380 394 413 425 436 439 445 459 468

3033 108 175 240 288 329 360 387 405 426 439 446 451 461 470 479 478

3034 113 175 231 283 335 363 398 424 443 462 482 498 516 532 545 553

3035 131 188 239 289 321 344 367 391 402 415 431 437 448 449 458 461

3036 123 191 249 307 352 385 413 436 457 479 497 511 519 533 537 548

3037 113 174 230 280 319 347 363 372 393 400 419 414 425 423 425 433

3038 137 207 262 314 360 383 410 425 460 472 501 516 526 535 540 553

3039 106 166 225 260 300 346 385 407 431 442 461 473 485 494 503 512

3040 109 172 228 285 328 367 403 433 455 477 502 506 530 542 551 559

3041 127 202 270 331 376 402 437 455 476 496 528 543 563 571 580 591

3042 103 162 211 257 302 341 368 385 405 424 437 450 463 475 487 497

3043 112 170 226 283 319 335 360 379 401 413 421 436 441 454 469 471

3 044 116 186 243 308 363 387 413 441 470 472 496 513 529 549 560 563

3045 110 175 241 294 345 380 412 428 449 462 469 483 497 493 512 524

3046 110 179 238 300 362 399 441 468 492 517 534 538 518 570 588 602

3047 126 189 246 295 335 358 386 408 430 454 474 493 499 508 519 519

3048 122 194 255 326 377 412 435 451 479 501 514 534 507 559 571 582

3049 131 194 249 303 341 372 398 411 433 452 468 484 489 502 515 526

3050 123 197 263 325 371 407 438 460 483 497 512 524 529 526 541 556

3051 114 186 248 314 369 407 435 455 492 505 525 543 560 577 588 586

MEAN 116 182 239 294 338 369 398 419 441 458 475 487 498 511 521 52 8

S.D. 9.2 11.9 15.5 20.5 24.8 25.8 28.8 31.0 34.3 35.9 39.9 42.1 42.2 47.1 47.6 49.4

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:04

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- 4206 PAGE 262

APPENDIX G

GASOLINE TBA VAPOR CO NDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

4026 112 178 234 287 328 362 391 428 433 447 465 470 487 503 494 507

4027 138 199 234 281 317 345 360 373 402 421 430 444 455 453 454 458

4028 109 181 239 295 328 364 394 419 441 463 483 492 481 500 515 532

4029 121 194 254 312 364 394 429 452 478 486 495 510 520 532 542 547

4030 114 181 234 287 324 357 383 418 431 448 470 488 502 513 520 512

4031 116 179 223 272 315 346 362 391 410 421 443 453 469 475 482 477

4032 102 162 215 266 314 352 384 413 437 454 468 479 485 483 504 525

4033 115 182 236 286 331 367 388 415 440 454 474 485 506 526 541 522

4034 108 172 220 265 300 330 356 383 387 403 421 427 439 445 453 471

4035 130 198 247 310 358 391 411 436 462 480 497 507 514 529 552 566

4052 101 166 213 269 307 331 367 414 424 433 452 461 476 490 493 498

4037 128 192 243 283 317 342 362 378 401 405 410 425 444 446 447 448

4038 123 191 243 301 345 381 408 434 456 464 485 485 503 507 511 523

4039 110 178 234 282 331 361 388 409 434 446 455 462 470 483 491 500

4040 106 169 218 264 307 336 362 380 398 415 427 437 448 454 460 470

4041 123 186 231 288 332 370 402 450 448 470 493 497 510 522 527 536

4042 132 193 243 286 323 344 372 406 417 428 440 443 446 451 461 464

4043 113 174 221 280 324 352 386 398 410 427 445 454 474 486 495 504

4044 112 179 234 286 335 364 400 432 459 483 505 521 528 538 554 5 54

4045 115 182 234 293 344 377 408 439 478 476 494 510 508 531 537 553

4046 106 172 225 281 333 357 378 407 416 431 451 459 462 460 471 478

4047 118 180 229 279 328 360 389 421 429 444 464 458 472 483 483 497

4048 110 176 230 279 328 368 387 405 424 441 454 458 458 463 476 483

4049 120 183 232 278 309 336 351 370 381 390 396 391 409 417 429 438

4050 104 170 228 279 315 340 371 422 428 430 441 456 482 478 495 488

4051 126 190 248 303 352 381 412 442 462 482 497 505 517 529 545 556

MEAN 116 181 232 284 327 358 385 413 430 444 460 468 479 488 497 504

S.D. 9.5 9.8 10.4 12.6 15.8 17.8 20.1 23.3 25.9 26.6 29.2 31.2 29.5 33.3 35.3 35. 8

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:04

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- 4206 PAGE 263

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STU DY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

1026 527 543

1027 500 516

1028 536 549

1029 541 555

1030 594 613

1031 520 522

1032 509 520

1033 712 742

1034 600 610

1035 562 566

1036 533 541

1037 567 581

1038 586 593

1039 542 546

1040 637 659

1041 557 566

1042 585 594

1043 558 574

1044 539 547

1045 444 449

1046 529 540

1047 585 600

1048 470 477

1049 550 562

1050 558 563

1051 591 613

MEAN 555 567

S.D. 52.2 56.8

N 26 2623

- JUN

- 2009 18:04

Huntingdon Life Sciences 00

- 4206 PAGE 264

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY ST UDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

2026 588 600

2027 570 587

2028 572 587

2029 636 643

2030 611 612

2031 555 561

2032 605 599

2033 637 658

2034 557 568

2035 541 551

2036 584 601

2037 545 553

2038 528 537

2039 590 603

2040 518 528

2041 566 574

2042 637 648

2043 538 551

2044 493 491

2045 531 535

2046 583 588

2047 543 564

2048 506 514

2049 530 538

2050 539 545

2051 537 548

MEAN 563 573

S.D. 39.7 41.1

N 26 2623

- JUN

- 2009 18:04

Huntingdon Life Sciences 00

- 4206 PAGE 265

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY S TUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

3026 461 471

3027 537 553

3028 605 629

3029 557 571

3030 598 614

303 1 486 499

3032 485 495

3033 490 496

3034 566 584

3035 463 477

3036 557 561

3037 452 463

3038 565 575

3039 524 528

3040 568 583

3041 599 625

3042 509 519

3043 477 492

3044 577 596

3045 525 545

3046 617 629

3047 534 544

3048 595 597

3049 539 546

3050 569 587

3051 596 602

MEAN 540 553

S.D. 49.7 51.6

N 26 2623

- JUN

- 2009 18:04

Huntingdon Life Sciences 00

- 4206 PAGE 266

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

4026 536 550

4027 480 498

4028 544 562

4029 554 564

4030 534 554

40 31 495 515

4032 540 557

4033 538 556

4034 474 486

4035 580 582

4052 513 522

4037 480 499

4038 531 545

4039 520 532

4040 481 493

4041 545 549

4042 474 488

4043 515 526

4044 572 590

4045 569 581

4046 491 506

4047 506 514

4048 498 508

4049 439 451

4050 500 512

4051 562 580

MEAN 518 531

S.D. 35. 9 35.7

N 26 2623

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 267

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

1526 121 155 187 210 223 24 3 256 263 266 279 277 272

1527 144 182 208 236 256 277 291 297 304 320 322 318

1528 130 179 204 224 2 54 267 280 282 297 308 299 316

1529 145 181 200 232 235 255 264 284 301 300 304 305 327 367 397 495

1530 139 189 211 235 253 258 280 289 297 309 327 312

1531 137 172 208 225 249 266 283 280 300 298 329 332

1532 134 165 190 214 234 251 257 264 275 277 278 283

1533 128 167 193 210 229 243 257 261 267 270 276 277

1534 132 164 191 211 230 242 250 253 264 272 277 281

1535 130 165 181 211 232 237 244 261 266 266 273 268

1536 132 167 188 207 218 235 251 255 256 262 271 269

1537 127 163 192 209 235 242 251 251 266 265 270 271

153 8 128 171 198 222 239 246 267 272 279 284 297 300

1539 120 157 181 208 222 239 257 269 268 277 277 284

1540 125 164 186 217 238 249 255 273 281 287 283 293

1541 122 157 178 204 221 220 237 248 255 252 265 260

1542 149 191 220 247 268 272 283 297 315 313 315 322

1543 123 160 191 216 234 244 255 264 274 284 286 297

1544 133 178 208 239 256 276 301 313 324 329 340 330

1545 142 182 214 232 264 273 292 293 316 327 323 341

1546 135 162 182 187 209 219 227 225 241 245 248 254

1547 123 155 183 201 215 220 231 236 252 256 256 264

1548 148 182 217 239 258 270 278 294 305 306 313 302 331 335 319 318

1549 135 173 196 226 241 244 265 265 282 284 292 292

1550 118 155 170 193 211 224 236 239 254 261 257 270 274 269 268 281

1551 122 161 176 207 227 238 252 245 267 26 4 277 268

MEAN 132 169 194 218 237 248 261 268 280 284 290 291 311 324 328 36 4

S.D. 9.0 10.9 13.7 15.1 16.5 17.7 19.6 21.5 22.6 23.7 25.0 24.6 32.1 49.8 64.9 114.4

N 26 26 26 26 26 26 26 26 26 26 26 26 3 3 3 323

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 268

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

2526 126 164 192 196 219 232 244 247 264 274 277 276

2527 136 170 191 221 244 253 255 267 276 273 272 278 278 289 294 308

2528 149 178 209 228 251 256 272 277 287 282 283 288

2529 137 172 204 229 246 272 289 297 295 304 307 301

2530 149 177 208 237 260 258 285 291 306 315 308 327 332

2531 124 15 4 189 209 219 238 252 265 261 266 297 312

2532 123 157 180 198 217 230 244 254 257 265 287 278

2533 1 28 161 188 210 234 247 250 267 280 284 284 295

2534 129 159 181 209 219 237 246 259 275 276 273 280

2535 132 165 193 222 232 240 256 268 275 295 321 306

2536 122 161 195 216 240 254 273 278 295 296 299 300

2537 122 154 181 194 218 231 240 246 253 260 253 255

2538 130 176 194 227 246 252 267 271 284 303 295 285 287 315 304 310

2539 134 167 195 217 234 242 259 268 279 282 286 288

2540 117 149 174 200 210 229 242 250 261 263 274 267

2541 144 191 231 270 290 313 324 340 358 347 358 364

2542 135 171 192 210 228 239 241 258 265 274 277 277 295 297 294 306

2543 144 184 212 233 244 260 284 292 309 324 324 333

2544 132 182 194 209 237 246 271 275 286 286 299 296

2545 133 167 191 214 232 242 258 264 271 277 283 285

2546 120 162 171 199 215 228 229 247 250 255 253 264

2547 120 146 171 189 205 212 224 234 237 238 248 248

2548 124 159 186 209 227 242 256 262 270 281 300 317

2549 130 163 191 206 233 245 259 279 278 290 305 296 348 a

2550 139 175 199 227 238 245 259 269 274 275 282 280

2551 141 176 203 222 246 265 280 284 296 307 315 318

MEAN 132 167 193 215 234 246 260 270 278 284 291 293 298 300 297 318

S.D. 9.0 10.9 13.4 16.8 17.7 18.8 21.2 21.0 23.6 23.1 24.1 25.3 23.9 13.5 5.7 20. 2

N 26 26 26 26 26 26 26 26 26 26 26 26 4 3 3 4

a Animal was pregnant but had total litter loss. Recording of body weights was resumed.23

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 269

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE : A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP III 10000 MG/M3

-------------------------------------------------------------------------------------------------------------------- ---------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

3526 135 173 204 219 242 256 255 272 279 288 283 293

3527 140 178 208 224 246 264 276 273 295 303 308 312

3528 138 166 184 207 216 222 236 238 256 265 280 273

3529 133 166 189 210 216 235 244 251 250 259 266 260

3530 118 157 184 194 224 236 247 257 274 264 275 290

3531 130 160 183 195 220 233 244 246 257 262 267 268

3532 148 178 202 225 241 256 262 271 282 280 294 301

3533 142 181 217 247 27 2 273 294 300 300 306 312 309

3534 136 169 195 206 227 242 246 248 260 262 260 271

3535 121 160 188 2 06 216 237 251 268 258 269 275 299

3536 124 173 191 218 239 247 255 271 282 284 281 295 295 a

3537 129 158 175 203 218 231 229 243 255 252 279 291

3538 130 167 200 222 240 255 264 273 282 280 304 311 300 305 293 307

3539 126 160 190 213 222 248 262 272 273 288 296 291 320 346 335 327

3540 122 154 181 198 218 237 248 258 268 274 277 281

3541 117 164 186 212 226 238 250 253 263 275 289 277

3542 149 189 219 238 257 272 284 292 294 308 320 316

354 3 125 159 178 198 215 218 233 238 243 262 275 269

3544 123 157 181 201 212 222 231 244 246 256 260 256

3545 121 150 181 199 221 228 246 244 261 272 286 272

3546 134 161 194 207 235 244 245 259 259 268 282 306

3547 127 153 177 183 209 207 226 239 247 244 256 259

3548 140 179 215 245 257 282 291 300 296 314 330 310 353 a

3549 131 164 196 220 234 246 243 264 269 266 273 273

3550 144 180 220 240 259 267 276 291 295 301 321 337 346 362 338 345

3551 133 169 210 221 248 249 268 270 288 300 294 303

MEAN 131 166 194 213 232 244 254 263 270 277 286 28 9 322 338 322 325

S.D. 9.1 10.1 13.8 16.3 16.9 18.1 18.6 18.8 17.5 19.1 19.8 20.6 22.9 29.7 25.5 24. 5

N 26 26 26 26 26 26 26 26 26 26 26 26 3 3 3 5

a Animal was pregnant but had total litter loss. Recording of body weights was resumed.23

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 270

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BOD Y

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

4526 130 157 179 199 223 238 255 259 271 279 277 272

4527 134 166 200 224 247 265 267 284 296 308 297 307

4528 123 157 178 207 228 238 259 273 280 278 288 283

4529 131 175 184 207 224 240 248 263 271 269 278 271

4530 134 169 182 200 201 229 235 234 252 262 260 263

4531 123 154 165 191 210 218 225 250 244 247 247 248

4532 131 159 174 184 211 215 220 241 249 246 249 261

4533 149 178 194 200 220 235 250 261 26 6 266 277 278

4534 147 182 201 224 240 250 269 279 284 276 290 287

4535 118 154 168 186 198 209 218 2 23 231 232 240 242

4536 134 162 172 207 222 235 241 260 260 266 259 275

4537 137 170 181 210 214 242 246 262 264 272 278 279

4538 121 153 180 200 213 230 240 254 249 259 270 262

4539 125 154 179 191 212 215 234 242 244 254 267 251

4540 126 153 168 191 210 211 230 237 249 252 248 257

4541 144 181 213 234 237 259 275 285 278 287 299 298 304 308 308 311

4542 118 160 185 213 217 242 248 259 269 261 279 283

4543 129 174 195 211 235 251 245 273 267 281 285 296

4544 124 160 175 202 217 227 232 244 249 250 250 258

4545 145 179 201 221 233 247 253 255 270 279 275 277

4546 139 178 191 224 242 251 251 275 283 286 281 299

4547 12 7 168 182 211 230 247 281 271 262 276 287 278 308 329 378

4548 135 178 204 216 244 259 269 273 287 292 294 309

4549 140 165 193 204 220 216 235

4550 132 159 190 208 233 243 254 262 269 275 278 287 300

4551 122 158 185 201 215 237 250 256 257 267 266 265

MEAN 131 165 185 206 223 236 247 259 264 269 273 275 304 318 343 31 1

S.D. 8.9 9.8 12.2 12.6 13.2 15.5 16.4 15.9 15.7 16.8 16.8 18.1 3.9 15.3 49.9 0. 0

N 26 26 26 26 26 26 26 25 25 25 25 25 3 2 2 123

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 271

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

1548 334

1550 285

MEAN 310

S.D. 34.6

N 223

- JUN

- 2009 18:05

Huntingdon Life Sc iences 00

- 4206 PAGE 272

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVI DUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

2527 315

2538 318

2542 316

2549 349 354 351 342

MEAN 324 354 351 342

S.D. 16.6 0.0 0.0 0.0

N 4 1 1 123

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 273

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 113 114

----------------------------------------------------------------------------------------------------------------------------- ------

3536 298 318 314 322

3538 312

3539 324

3548 364 340 348 339

3550 351

MEAN 330 329 331 331

S.D. 27.4 15.7 24.0 11.6

N 5 2 2 223

- JUN

- 2009 18:05

Huntingdon Life Sciences 00

- 4206 PAGE 274

APPENDIX G

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RAT S

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 113 114

-----------------------------------------------------------------------------------------------------------------------------------

4541 325

MEAN 3 25

S.D. 0.0

N 123

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 275

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

1026 59 56 51 40 28 23 22 20 23 16 17 6 12 4 5 12

1027 63 56 54 45 27 26 15 22 14 25 10 6 13 13 4 0

1028 71 53 51 39 28 22 22 25 9 5 19 15 15 24 1 19

1029 72 57 47 51 29 36 27 26 10 9 17 4 13 3 8 12

1030 69 60 55 54 28 30 33 25 19 23 17 12 19 10 9 11

1031 63 54 60 49 27 27 30 24 12 16 10 10 8 2 6 17

1032 66 49 51 33 23 24 21 28 10 16 25 7 15 7 3 8

1033 81 66 76 67 43 51 31 37 22 19 28 5 28

- 7 25 20

1034 69 58 64 58 35 46 21 21 20 26 12 25 14 10 16

- 4

1035 70 47 60 43 24 32 19 14 3 20 18 10 15 11 36 10

1036 67 49 57 55 31 40 19 20 15 25

- 3 9 15 6 8 7

1037 72 56 66 52 36 29 24 31 9 14 14 1 9 14 26 0

1038 75 65 67 60 26 29 28 30 3 10 8 22 15 8 18 5

1039 70 50 61 46 21 36 25 22 14 17 14 18 12 12 15 1

1040 72 64 69 59 41 29 24 27 18 17 16 24 13 13 23 13

1041 65 52 55 43 36 35 21 21 20 11 22 21 7 19 18 8

1042 71 5 6 52 49 32 40 22 30 15 15 15 17 11 16 10 11

1043 64 59 47 44 31 37 17 29 8 13 18 10 14 16 9 14

1044 74 57 52 52 25 37 21 12 7 16 13 10 13 13 17 8

1045 64 45 50 35 10 19 11 17 15 9 1 11 12 15 11 11

1046 64 52 56 53 32 29 23 24 16 20 7 19 14 8 3 1

1047 67 64 71 56 34 32 30 17 18 26 7 3 18 12 8 9

1048 66 53 59 44 23 23 16 13 17 17

- 1 2 1 9 13 12

1049 64 48 65 51 35 29 28 21

- 4 14 13 23 16 17 13 12

1050 73 67 58 48 30 26 28 23 13 14 16 12 1 15 11 8

1051 76 64 65 50 23 31 24 18 19 14 19 8 16 15 9 8

MEAN 69 56 58 49 29 32 23 23 13 16 13 12 13 11 13 9

S.D. 4.9 6.3 7.8 7.9 6.8 7.4 5.3 5.9 6.4 5.6 7.2 7.2 5.3 6.2 8.3 5.9

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 276

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ON E- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP II 2000 MG/M3

-------------------------------------------------------------------------------------------------------------------------- ---------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

2026 70 59 55 44 46 35 31 19 17 18 1 4 19 13 12 14

2027 62 49 58 54 37 38 29 27 9 18 16 16 10 11 6 19

2028 71 57 63 47 41 35 19 33 20 17 9 2 13 11 11 6

2029 69 58 61 52 42 45 35 24 22 27 12 23 13 18 8 19

2030 73 55 61 50 40 33 29 29 18 23 18 7 5 18 11 16

2031 70 56 59 45 35 31 22 25 13 17 9 8 12 11 16 0

2032 70 55 53 47 33 21 35 21 26 18 12 24 16 15 9 29

2033 65 65 66 44 36 36 37 19 20 18 20 17 22 10 20 17

2034 59 53 59 45 24 40 25 18 21 18 17 10 12 13 8 25

2035 66 55 60 45 34 36 22 19 14 18 10 11 14 4 11 20

2036 77 59 72 45 32 25 22 16 17 22 18 2 17 6 13 11

2037 65 60 5 7 50 22 35 24 28 15 18 11 14 15 8 0 16

2038 65 55 51 37 34 19 30 16 13 25 7 18 19 10 8 13

2039 71 61 61 49 38 33 31 15 26 11 11 5 29 14 7 8

2040 63 47 46 40 35 30 21 26 17 18 12

- 4 29 16 17 1

2041 69 58 53 49 36 40 18 20 18 19

- 2 18 5 10 19 2

2042 71 67 65 63 42 37 29 29 15 17 24 6 12 14 13 18

2043 66 51 57 55 27 35 23 24 9 6 11 15 18 14 10 13

2044 64 51 48 36 14 27 20 17 19 20 10 17 11 12 9 15

2045 68 59 57 47 28 32 9 25 9 17 9 6 4 16 11 14

2046 73 59 65 55 31 31 25 17 12 14 15 11 7 7 19 26

2047 69 57 60 40 29 43 19 26 16 19 7 15 3 6 9 11

2048 70 53 58 40 31 28 10 18 7 13 7 16 5 1 1 4 18

2049 62 53 48 48 32 31 23 28 9 13 18 9 10 2 19 11

2050 56 58 64 40 39 31 19 17 13 29 11 24 13 5 6 10

2051 66 54 60 36 31 31 25 18 14 19 25 6 29 11 6

- 4

MEAN 67 56 58 46 33 33 24 22 16 18 12 11 14 11 11 13

S.D. 4.6 4.5 6.0 6.5 6.9 6.0 6.9 5.1 5.0 4.8 6.3 7.3 7.4 4.5 5.0 7. 8

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 277

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 4 9- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

3026 61 44 46 31 28 19 13 19 17 6 12 19 10 8 11 14

3027 67 55 58 43 19 37 25 8 18 14 14 20 18

- 5 5 25

3028 69 54 68 46 32 35 25 26 26 28 20 18 7 2 20 14

3029 66 65 54 52 29 32 18 23 23 12 11 14 17 6 2 16

3030 62 56 58 51 39 37 37 25 18 24 7 29 15 15 7 17

3031 69 50 43 38 25 30 20 17 18 12 12 11 9 8

- 6 11

3032 65 45 54 4 1 25 23 21 14 20 11 11 3 6 14 9 17

3033 68 65 48 41 31 28 18 21 12 7 5 10 9 10

- 1 12

3034 62 57 52 52 29 34 27 19 19 20 16 19 15 13 8 13

3035 57 51 51 32 22 24 24 11 13 16 6 11 1 10 3 2

3036 68 58 58 45 33 29 23 21 23 18 14 8 14 4 11 9

3037 61 57 50 39 28 16 9 21 7 19

- 5 11

- 2 2 8 19

3038 71 55 52 46 23 27 14 36 11 29 15 10 10 4 13 12

3039 60 59 35 40 46 39 23 23 12 19 12 12 9 10 9 13

3040 63 56 57 44 38 36 30 22 21 25 5 24 13 9 8 9

3041 75 68 61 46 26 35 18 21 20 32 15 19 8 9 12 8

3042 59 49 46 45 39 27 17 20 19 13 12 13 12 13 10 12

3043 58 55 57 36 17 24 19 22 13 8 14 5 13 15 2 6

3044 71 56 66 55 23 26 28 29 3 24 17 16 20 12 3 13

3045 66 66 52 52 34 32 16 21 13 7 13 15

- 4 19 12 1

3046 69 60 62 62 37 43 27 24 25 17 5

- 21 52 18 15 15

3047 62 57 50 40 23 28 22 22 24 20 19 6 9 11 1 15

3048 72 62 70 52 34 24 16 28 22 13 19

- 27 53 12 11 14

3049 62 56 54 38 31 26 13 22 19 17 15 5 13 13 11 13

3050 74 66 62 46 35 32 22 23 14 15 12 5

- 4 16 15 13

3051 72 62 66 54 39 28 20 37 13 20 18 17 17 11

- 2 10

MEAN 66 57 55 45 30 30 21 22 17 17 12 10 13 10 7 1 2

S.D. 5.1 6.2 8.1 7.3 7.2 6.3 6.0 6.2 5.7 6.9 5 .6 11.8 13.2 5.3 5.9 4.9

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 278

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

4026 65 56 53 41 33 29 37 5 15 17 5 18 16

- 10 14 29

4027 62 35 47 37 2 8 15 14 29 19 9 14 11

- 2 0 4 22

4028 72 58 55 33 36 30 25 22 22 20 9

- 11 19 15 18 12

4029 73 60 58 51 30 35 23 25 9 9 15 10 12 10 5 7

4030 67 53 53 37 33 26 36 13 17 22 18 13 11 7

- 8 22

4031 62 45 49 43 31 17 29 19 11 22 10 17 5 8

- 5 18

4032 60 53 52 48 37 32 29 24 17 14 11 5

- 1 21 21 15

4033 67 54 50 45 36 21 27 25 15 19 11 21 21 15

- 20 16

4034 65 48 45 35 30 26 27 4 16 18 6 12 6 8 18 3

4035 68 48 64 48 33 20 24 26 18 17 10 7 15 23 15 13

4052 64 47 56 38 25 36 47 10 9 19 9 15 14 3 5 15

4037 64 51 40 35 25 20 17 23 3 5 15 19 2 1 1 32

4038 68 52 58 44 37 26 26 22 8 21 0 18 4 4 11 8

4039 68 56 48 49 30 27 21 25 13 9 7 8 13 8 10 20

4040 63 49 46 43 29 26 19 18 17 12 9 12 5 6 10 11

4041 63 45 58 43 38 33 48

- 2 22 23 4 14 11 5 10 9

4042 62 49 43 37 22 28 34 10 11 12 3 4 5 10 3 10

4043 61 46 59 44 28 35 12 12 17 18 9 20 12 8 9 11

4044 68 55 52 50 29 36 32 27 25 21 16 7 10 16

- 1 19

4045 67 51 59 51 33 32 31 38

- 2 18 17

- 2 23 6 16 16

4046 67 53 56 52 24 21 29 9 16 19 9 2

- 1 10 7 13

4047 62 48 50 49 32 29 32 8 15 20

- 6 15 11 0 15 8

4048 66 54 50 49 40 20 18 19 17 13 4 0 5 13 7 15

4049 63 49 46 30 27 16 18 11 10 5

- 4 18 8 12 8 1

4050 66 58 51 36 26 30 51 6 2 11 15 26

- 4 17

- 7 12

4051 64 58 55 49 30 31 29 21 19 15 9 12 12 16 11 6

MEAN 65 51 52 43 31 27 28 17 14 16 9 11 9 9 7 14

S.D. 3.3 5.4 5.7 6.5 4.7 6.4 9.9 9.4 6.3 5.3 6.1 8.2 7.0 7.1 9.2 7. 2

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 279

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCT ION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

1026 16 295 350

1027 16 295 347

1028 13 273 360

1029 14 307 362

1030 19 343 423

1031 2 309 354

1032 10 280 330

1033 30 439 540

103 4 10 360 431

1035 4 281 368

1036 7 309 362

1037 14 330 394

1038 8 326 402

1039 3 306 368

1040 22 365 474

1041 9 315 397

1042 10 325 399

1043 16 302 381

1044 8 292 361

1045 5 212 275

1046 10 313 367

1047 14 354 419

1048 7 264 307

1049 12 301 392

1050 5 323 375

1051 22 326 404

MEAN 12 313 382

S.D. 6.6 41.1 51.3

N 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 280

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

2026 12 325 400

2027 17 334 412

2028 15 341 398

2029 7 378 464

2030 1 355 411

2031 6 313 366

2032

- 6 322 409

2033 22 360 468

2034 11 319 397

203 5 10 310 380

2036 17 327 392

2037 8 319 379

2038 9 287 364

2039 14 335 411

2040 11 291 360

2041 8 308 370

2042 11 389 462

2043 13 297 379

2044

- 2 262 323

2045 4 291 345

2046 5 325 400

2047 21 315 381

2048 9 264 328

2049 9 304 363

2050 6 322 385

2051 11 313 371

MEAN 9 319 389

S.D. 6.3 29.9 36.5

N 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 281

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXI CITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

3026 11 236 307

3027 16 292 371

3028 25 358 444

3029 14 318 386

3030 16 350 447

3031 13 266 313

3032 10 265 324

3033 6 276 321

3034 18 323 409

3035 14 249 289

303 6 4 320 370

3037 11 240 289

3038 10 308 368

3039 3 307 362

3040 16 334 411

3041 26 342 423

3042 10 288 357

3043 14 265 321

3044 20 327 410

3045 20 307 369

3046 12 360 451

3047 10 304 355

3048 1 340 403

3049 8 290 353

3050 18 327 390

3051 6 357 416

MEAN 13 306 371

S.D. 6.2 36.9 47.9

N 26 26 2623

- JUN

- 2009 18:12

Huntingdon Life Sciences 00

- 4206 PAGE 282

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

4026 14 292 372

4027 18 245 299

4028 18 310 381

4029 10 316 370

4030 20 307 372

4031 20 274 336

4032 17 318 395

4033 19 303 375

4034 12 255 314

4035 3 309 384

4052 9 296 357

403 7 19 234 307

4038 14 294 354

4039 12 284 354

4040 12 268 324

4041 5 311 364

4042 15 249 295

4043 11 280 351

4044 18 342 411

4045 13 328 399

4046 15 287 334

4047 8 277 333

4048 10 282 332

4049 12 209 268

4050 12 285 342

4051 18 315 390

MEAN 13 287 350

S.D. 4.6 30.7 35.5

N 26 26 2623

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 283

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STU DY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

1526 34 32 24 12 21 1 3 8 2 13

- 2

- 5

1527 38 26 28 20 21 14 6 7 15 2

- 5

1528 49 25 20 30 13 14 2 15 11

- 9 17

1529 36 19 32 3 20 9 20 17

- 1 4 2 22 40 30 98

1530 51 22 24 18 5 22 8 8 13 18

- 16

1531 35 36 17 24 17 17

- 4 20

- 2 31 3

1532 31 25 25 19 18 6 8 11 2 1 5

1533 39 26 17 19 14 13 5 6 3 6 1

1534 32 28 19 19 12 8 3 11 8 4 5

1535 35 16 30 21 5 7 18 5

- 1 7

- 5

1536 35 21 19 11 17 16 4 1 7 9

- 2

1537 36 29 17 26 7 9 0 15

- 1 5 1

153 8 42 27 24 18 6 21 5 7 5 13 3

1539 37 24 27 14 17 18 12

- 1 9 0 7

1540 40 22 30 22 11 6 17 9 6

- 4 10

1541 35 21 26 17

- 2 17 11 6

- 3 13

- 5

1542 42 29 27 20 5 10 14 19

- 2 3 7

1543 37 31 26 18 10 11 9 10 10 2 11

1544 45 30 31 17 20 25 12 11 5 10

- 10

1545 40 32 18 33 9 18 2 23 10

- 4 18

1546 27 19 6 22 10 7

- 2 16 4 4 5

1547 31 29 18 14 4 11 5 16 4 0 9

1548 33 35 22 19 12 9 16 10 1 7

- 11 29 4

- 17

- 1 16

1549 38 23 30 15 2 21 0 18 1 8 1

1550 37 15 23 19 13 12 3 15 7

- 4 12 4

- 5

- 1 12 5

1551 39 15 31 20 11 14

- 7 22

- 3 1 3

- 9

MEAN 37 25 24 19 11 13 7 11 5 5 2 18 13 4 36 1 1

S.D. 5.3 5.8 6.1 5.8 6.2 5.3 6.8 6.4 5.4 8.1 8.5 13.0 23.3 23.9 53.5 8.1

N 26 26 26 26 26 26 26 26 26 26 26 3 3 3 3 223

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 284

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

2526 38 28 5 22 13 12 4 17 9 4

- 1

2527 34 21 30 23 9 2 12 9

- 3 0 6

- 1 11 6 13 7

2528 29 31 19 23 5 16 5 10

- 5 0 5

2529 35 33 25 17 26 17 9

- 2 9 4

- 6

2530 29 31 28 23

- 2 27 6 16 9

- 8 19 6

2531 30 35 20 10 19 14 13

- 5 6 31 14

2532 34 23 18 19 13 15 9 4 8 22

- 9

2533 33 27 22 24 13 3 17 13 4 0 11

2534 30 23 28 10 18 8 13 16 1

- 3 7

253 5 33 28 29 10 8 16 12 7 20 26

- 15

2536 39 34 21 24 15 19 5 17 0 4 1

2537 33 27 13 24 13 10 6 6 8

- 8 2

2538 47 17 33 19 6 15 4 13 20

- 8

- 10 2 28

- 11 6 8

2539 33 28 23 17 8 17 9 11 3 4 2

2540 32 25 25 10 19 13 8 12 1 12

- 8

2541 46 41 38 20 23 11 16 18

- 11 12 5

2542 36 21 19 18 11 2 17 8 8 3 0 18 2

- 3 12 9

2543 40 28 21 11 17 23 9 17 15 0 9

2544 50 13 15 28 10 24 5 11

- 1 13

- 2

2545 34 24 23 18 9 16 7 6 7 6 2

2546 42 10 28 16 13 1 18 3 6

- 2 11

2547 26 24 18 15 8 12 10 3 1 10 1

2548 34 27 23 19 14 14 6 7 12 1 9 16

2549 33 28 15 27 11 15 20

- 1 12 15

- 9 1 a

2550 36 24 28 11 7 14 10 5 2 7

- 3

2551 35 26 19 25 18 15 4 12 11 8 3

MEAN 35 26 22 19 12 14 10 9 6 6 2 6 14

- 3 10 6

S.D. 5.6 6.6 6.9 5.6 5.9 6.5 4.8 6.3 7.2 10.2 8.5 8.3 13.4 8.4 4.1 3. 7

N 26 26 26 26 26 26 26 26 26 26 26 4 3 3 3 4

a Animal was pregnant but had total litter loss. Recording of body weights was resumed.23

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 285

APPENDIX H

GASOLINE TBA VAPOR CONDENSA TE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP III 10000 MG/M3

------------------------------------------------------------------------------------------------------------------ -----------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

3526 38 31 15 23 14 0 16 8 8

- 4 10

3527 38 29 17 22 18 12

- 3 22 8 5 4

3528 28 18 23 8 7 14 2 18 9 15

- 7

3529 32 24 21 7 18 9 7 0 9 7

- 6

3530 39 27 10 30 12 11 11 16

- 10 11 15

3531 31 23 11 26 13 10 3 11 4 5 1

3532 29 24 23 17 14 7 9 11

- 2 14 7

3533 39 36 29 25 1 22 6 0 6 6

- 4

3534 34 25 12 21 14 4 2 12 2

- 2 10

3535 39 28 1 9 10 21 14 17

- 9 11 5 25

3536 50 18 27 21 8 8 16 10 2

- 3 13 3 a

3537 28 17 28 16 12

- 1 13 12

- 3 27 12

3538 37 33 22 18 15 9 8 9

- 2 24 7

- 10 4

- 12 15 5

3539 35 29 24 9 25 15 10 1 15 8

- 5 29 26

- 11

- 8

- 3

3540 32 27 17 21 18 11 10 10 6 3 4

3541 47 22 27 14 12 12 2 10 12 14

- 12

3542 40 29 20 19 15 12 9 2 15 12

- 4

3543 34 19 20 17 3 15 5 5 19 14

- 7

3544 34 24 20 12 10 9 12 3 10 4

- 5

3545 29 31 18 22 7 18

- 2 17 11 14

- 13

3546 27 33 13 28 9 1 14 0 9 14 24

3547 26 24 7 26

- 2 18 13 8

- 3 12 4

3548 39 35 30 13 25 9 9

- 3 18 16

- 20 12 a

3549 33 32 24 14 12

- 3 21 6

- 3 7 0

3550 36 40 20 19 8 9 16 4 6 19 16 9 17

- 24 6 6

3551 37 40 12 27 1 19 2 18 12

- 6 9

MEAN 35 28 19 19 12 10 9 8 7 9 3 9 16

- 16 4 5

S.D. 5.8 6.5 6.3 6.4 6.9 6.3 6.2 7.4 7.2 8.1 11.1 19.5 10.9 7.1 11.4 5. 1

N 26 26 26 26 26 26 26 26 26 26 26 3 3 3 3 5

a Animal was pregnant but had total litter loss. Recording of body weights was resumed.23

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 286

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP I V 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

4526 27 22 20 25 15 16 5 12 8

- 2

- 4

4527 32 33 24 23 18 2 17 12 12

- 11 11

4528 34 21 28 21 10 22 13 7

- 2 10

- 5

4529 44 9 23 17 16 8 14 9

- 3 9

- 7

4530 35 13 18 2 27 6

- 1 18 9

- 1 2

4531 32 11 26 19 8 7 25

- 6 3

- 1 1

4532 28 15 10 27 4 5 21 8

- 2 2 13

4533 29 15 6 20 15 15 11 5 0 12 1

4534 35 20 23 15 10 19 11 5

- 8 14

- 3

4535 36 14 18 12 12 9 5 8 1 8 2

4536 29 10 34 16 13 6 19 0 5

- 7 16

4537 33 12 28 5 28 4 15 2 8 7 1

4538 32 27 20 13 17 10 14

- 5 10 11

- 7

4539 29 25 12 21 4 19 8 2 10 13

- 16

4540 28 15 23 19 2 19 7 12 3

- 4 9

4541 37 33 20 4 22 16 10

- 7 9 11

- 1 6 4 0 3 14

4542 42 25 27 4 25 6 11 10

- 8 17 4

4543 45 22 15 24 17

- 7 28

- 5 14 4 11

4544 36 15 28 15 9 5 12 5 1 1 8

4545 3 4 22 20 13 13 6 2 16 9

- 4 2

4546 39 13 33 18 9 0 24 8 3

- 5 17

4547 41 14 29 19 17 34

- 10

- 10 15 11

- 9 30 21 49

4548 43 26 13 27 15 10 4 14 5 2 15

4549 24 28 12 15

- 4 19

4550 27 32 18 25 11 10 8 7 6 3 9 13

4551 36 27 16 14 22 13 6 2 9 0

- 1

MEAN 34 20 21 17 14 11 11 5 5 4 3 16 13 25 3 14

S.D. 5.7 7.4 7.2 7.1 7.7 8.3 8.5 7.4 6.2 7.4 8.5 12.1 12.4 34.6 0.0 0. 0

N 26 26 26 26 26 26 25 25 25 25 25 3 2 2 1 123

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 287

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

1526 117

1527 136

1528 136

1529 125

1530 122

1531 160

1532 119

1533 110

1534 118

1535 103

1536 102

1537 108

1538 129

1539 128

1540 129

1541 103

1542 13 1

1543 137

1544 151

1545 159

1546 91

1547 110

1548 120

1549 119

1550 115

1551 107

MEAN 122

S.D. 17.2

N 2623

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 288

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0- 69

-----------------------------------------------------------------------------------------------------------------------------------

2526 112

2527 108

2528 110

2529 130

2530 149

2531 157

2532 121

2533 134

2534 121

2535 141

2536 139

2537 100

2538 108

2539 122

2540 117

2541 173

2542 106

2543 149

2544 115

2545 118

2546 103

2547 102

2548 158

2549 133

2550 104

2551 142

MEAN 126

S.D. 20.1

N 2623

- JUN

- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 289

APPENDIX H

GASOLIN E TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

3526 121

3527 133

3528 107

3529 94

3530 132

3531 108

3532 123

3533 128

3534 101

3535 140

3536 121

35 37 133

3538 144

3539 131

3540 127

3541 113

3542 126

3543 110

3544 99

3545 123

3546 146

3547 106

3548 131

3549 110

3550 157

3551 133

MEAN 123

S.D. 15.6

N 2623

- JU N- 2009 18:25

Huntingdon Life Sciences 00

- 4206 PAGE 290

APPENDIX H

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

4526 116

4527 141

4528 126

4529 96

4530 94

4531 94

4532 103

4533 100

4534 105

4535 88

4536 113

4537 109

4538 110

4539 96

4540 104

4541 117

4542 123

4543 122

4544 98

4545 98

4546 121

4547 110

4548 131

4550 128

4551 107

MEAN 110

S.D. 13.6

N 2523

- JUN

- 2009 18:28

Huntingdon Life Sciences 00

- 4206 PAGE 291

APPENDIX I

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

1526 270 297 305 338 401

1527 DPP 320 343 362 385 453

1528 304 334 347 373 442

1529xP NMD

1530 305 329 340 370 423

1531 330 347 356 388 457

1532x NP 274 298 300 321 317

1533 272 299 305 331 362

1534 278 296 304 340 403

1535x NP 259 285 300 326 299

1536 282 307 315 344 392

1537 265 284 284 320 379

1538 297 314 327 351 424

1539 287 313 316 352 402

1540 298 319 327 356 430

1541x NP 263 283 289 297 283

1542 325 344 350 382 445

1543 305 322 335 362 439

1544 335 375 393 427 460

1545 341 365 379 410 485

1546 251 275 288 319 379

1547 258 278 291 313 379

1548xNNMD

1549 282 312 322 345 404

1550xNNMD

1551 272 291 293 324 398

MEAN 294 317 327 357 418

S.D. 26.4 27.9 30.6 30.8 33.0

N 20 20 20 20 20

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:28

Huntingdon Life Sciences 00

- 4206 PAGE 292

APPENDIX I

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3

------------------------------------------------------------------------------------------------------------------------ -----------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

252 6 265 291 303 330 394

2527xNNMD

2528 288 310 320 340 419

2529 303 325 336 366 422

2530 327 372 383 417 473

2531 290 317 328 352 436

2532x NP 275 280 286 287 289

2533 293 316 325 351 421

2534 268 298 309 333 380

2535 306 330 340 369 433

2536 298 331 338 375 447

2537 252 283 297 333 385

2538xNNMD

2539 280 300 312 349 404

2540 271 293 307 330 381

2541 356 387 401 432 481

2542xNNMD

2543 326 344 352 371 442

2544 301 333 344 367 405

2545 288 313 325 348 392

2546 255 275 292 315 384

2547 243 263 268 296 357

2548 307 324 329 357 378

2549 TLL 291 326 335 352 369

2550 282 305 312 333 382

2551 309 340 353 386 435

MEAN 291 317 328 355 410

S.D. 26.5 29.2 29.2 30.9 33.6

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:28

Huntingdon Life Sciences 00

- 4206 PAGE 293

APPENDIX I

GASOLINE TBA VAPOR CON DENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

----------------------------------------------------------------------------------------------------------------------------- ------

3526x NP 299 297 301 305 307

3527 301 346 353 388 470

3528 267 290 293 328 378

3529 260 288 298 334 406

3530 280 316 325 352 409

3531 258 288 301 343 422

3532 295 321 329 356 427

3533 302 327 337 366 433

3534 264 294 310 335 409

3535 305 321 329 353 399

3536 TLL 291 313 324 347 326

3537 270 298 303 332 402

3538xNNMD

3539xNNMD

3540 274 305 314 349 368

3541 280 302 316 336 393

3542 318 342 347 384 457

3543 270 292 299 303 3 91

3544 259 282 290 314 368

3545 276 293 301 33 2 400

3546 294 317 324 351 426

3547 259 296 303 335 405

3548 TLL 319 347 362 381 443

3549 273 299 312 349 424

3550xNNMD

3551 303 327 344 369 407

MEAN 283 309 319 347 407

S.D. 19.5 19.7 20.4 21.3 31.5

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:28

Huntingdon Life Sciences 00

- 4206 PAGE 294

APPENDIX I

GASOLINE TB A VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

4526 284 301 309 340 404

4527 298 336 340 374 438

4528 292 313 318 352 422

4529 279 305 317 351 404

4530x NP 256 277 295 306 272

4531 254 267 274 300 356

4532 259 289 296 332 405

4533 278 307 324 359 434

4534 300 319 332 365 435

4535 243 257 267 293 346

4536 263 285 295 335 358

4537 286 317 330 349 403

4538 273 288 301 329 408

4539x NP 249 264 281 290 262

4540 253 285 297 319 368

4541xNNMD

4542 273 302 306 337 399

4543 286 313 318 357 410

4544 262 282 296 326 402

4545 278 302 310 342 390

4546 279 319 325 355 430

4547xPNMD

4548 289 316 328 357 432

4549 DIED

4550 290 322 332 368 436

4551 261 287 301 323 381

MEAN 275 301 310 341 403

S.D. 15.8 19.5 19.1 21.2 28.0

N 21 21 21 21 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:30

Huntingdon Life Sciences 00

- 4206 PAGE 295

APPENDIX J

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

1526 27 8 33 63 131

1527 DPP 23 19 24 68 134

1528 30 12 26 70 138

1529xPNMD

1530 24 11 30 53 119

1531 17 9 32 69 127

1532x NP 24 2 21

- 4 44

1533 27 6 26 31 90

1534 17 8 36 63 125

1535x NP 26 14 27

- 27 40

1536 24 9 29 48 110

1537 19 1 36 59 114

1538 17 13 24 73 127

1539 26 3 37 50 116

1540 21 8 29 75 132

1541x NP 20 6 8

- 14 21

1542 20 5 33 62 120

1543 16 14 27 77 134

1544 41 18 34 33 125

1545 24 15 31 74 144

1546 24 13 32 60 128

1547 20 13 21 66 120

1548xNNMD

1549 30 10 23 59 122

1550xNNMD

1551 19 1 31 74 126

MEAN 23 10 30 61 124

S.D. 5.9 5.0 4.6 12.9 11.5

N 20 20 20 20 20

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:30

Huntingdon Life Sciences 00

- 4206 PAGE 296

APPENDIX J

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENE RATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROU P II 2000 MG/M3

----------------------------------------------------------------------------------------------------------------------------- ------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

2526 25 12 28 64 128

2527xNNMD

2528 22 10 19 80 131

2529 23 11 30 56 119

253 0 44 12 34 56 146

2531 28 10 25 83 146

2532x NP 5 7 0 2 14

2533 22 9 26 70 128

2534 30 12 24 47 112

2535 25 10 29 63 127

2536 33 7 36 73 150

2537 31 15 36 52 133

2538xNNMD

2539 20 12 36 56 124

2540 22 14 23 50 109

2541 31 14 31 50 125

2542xNNMD

2543 18 8 19 71 116

2544 32 11 23 38 104

2545 24 12 22 44 103

2546 21 17 22 70 130

2547 19 5 28 62 114

2548 17 5 28 21 71

2549 TLL 35 9 17 17 79

2550 23 7 21 49 100

2551 31 13 34 49 126

MEAN 26 11 27 55 119

S.D. 6.6 3.1 5.9 16.6 19.7

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:30

Huntingdon Life Sciences 00

- 4206 PAGE 297

APPENDIX J

GASOLINE TBA VAPOR CONDENSATE: A ONE -G ENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

G ROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

3526x NP

- 2 4 4 1 8

3527 44 8 34 83 169

3528 23 4 35 50 112

3529 28 10 36 72 147

3530 36 9 27 57 129

3531 30 13 41 80 164

3532 26 9 27 71 132

3533 26 10 28 67 131

3534 30 16 25 75 145

3535 16 8 25 45 94

3536 TLL 22 10 23

- 21 35

3537 28 5 29 71 133

3538xNNMD

3539xNNMD

3540 31 9 36 18 94

3541 22 14 20 57 113

3542 24 5 37 73 139

3543 22 8 4 88 122

3544 23 8 25 53 108

3545 17 8 32 68 124

3546 23 7 27 75 132

3547 37 7 32 70 147

3548 TLL 28 15 18 63 124

3549 26 14 36 75 150

3550xNNMD

3551 25 16 25 3 8 104

MEAN 27 10 28 60 125

S.D. 6.5 3.6 8.1 24.1 28.4

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:30

Huntingdon Life Sciences 00

- 4206 PAGE 298

APPENDIX J

GASOLINE TBA VAPOR CONDENSATE: A ON E- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROUP IV 20000 MG/M3

-------------------------------------------------------------------------------------------------------------------------- ---------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

4526 17 8 31 64 120

4527 38 4 35 64 139

4528 21 4 34 71 130

4529 26 13 33 53 125

4530x NP 21 18 11

- 34 16

4531 13 7 26 56 102

4532 30 7 36 73 146

4533 30 17 35 75 157

4534 18 14 33 70 134

4535 14 10 26 53 103

4536 22 10 39 24 95

4537 31 13 19 54 117

4538 16 12 28 79 136

4539x NP 16 17 9

- 28 13

4540 32 12 22 50 115

4541xNNMD

4542 30 4 31 61 126

4543 28 5 39 53 124

4544 20 14 30 76 140

4545 23 8 32 48 112

4546 40 6 30 75 151

4547xPNMD

4548 27 12 29 75 143

4549 DIED

4550 32 10 36 67 146

4551 26 14 22 57 120

MEAN 25 10 31 62 128

S.D. 7.4 3.8 5.5 13.2 16.9

N 21 21 21 21 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:32

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APPENDIX K

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

1526 296 318 290 336 318 319

1527 DPP

1528 339 357 371 387 382 350

1529 PNMD a 363 375 391 398 358

1530 326 332 359 369 365 341

1531 366 362 376 399 369 347

1532x NP

1533 301 315 332 356 335 340

1534 317 319 345 361 353 317

1535x NP

1536 308 327 337 357 373 322

1537 286 303 326 350 326 302

1538 3 19 312 343 362 348 320

1539 331 337 360 371 354 330

1540 326 335 352 361 350 320

1541x NP

1542 341 342 368 382 365 347

1543 331 341 352 372 358 320

1544 361 387 422 427 399 360

1545 384 387 403 416 391 381

1546 288 279 313 341 309 299

1547 305 287 319 345 326 309

1548xNNMD

1549 315 311 344 371 356 332

1550xNNMD

1551 308 328 318 356 327 286

MEAN 324 332 350 370 355 330

S.D. 26.3 29.1 31.1 23.9 26.2 23.4

N 19 20 20 20 20 20

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

x=E XCLUDED FROM MEAN

a Body weight was not taken on Lactation Day 1 due to technician error.23

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- 2009 18:32

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APPENDIX K

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

2526 291 295 300 345 340 320

2527xNNMD

2528 301 308 324 348 352 315

2529 318 324 344 353 356 348

2530 380 398 419 434 425 371

2531 299 319 328 342 336 310

2532x NP

2533 324 312 287 361 354 334

2534 279 279 307 313 320 295

2535 325 340 355 359 355 349

2536 338 358 352 367 360 338

2537 312 328 318 350 339 308

2538xNNMD

2539 316 323 332 354 338 344

2540 298 307 319 331 342 316

2541 388 373 342 398 386 392

2542xNNMD

2543 334 345 380 373 349 343

2544 330 341 349 355 344 337

2545 311 326 343 337 335 319

2546 291 296 292 338 321 292

2547 261 289 300 317 324 286

2548 322 337 343 358 351 342

2549 TLL 335

2550 322 321 337 349 355 325

2551 336 345 346 368 379 333

MEAN 319 327 334 355 351 329

S.D. 29.0 28.3 30.3 26.0 23.7 25.6

N 22 21 21 21 21 21

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

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APPENDIX K

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 14 21 28

-------------------------------------------------------------------------------------------------------------------------------- ---

3526x NP

3527 347 345 376 392 365 355

3528 294 314 317 335 338 307

3529 302 315 331 355 345 305

3530 319 331 354 363 323 325

3531 317 320 342 366 342 318

3532 338 355 376 401 381 362

3533 321 358 343 389 380 357

3534 321 314 332 374 343 313

3535 325 330 341 358 355 345

3536 TLL

3537 281 286 319 339 333 312

3538xNNMD

3539xNNMD

3540 318 299 324 335 342 321

3541 324 325 337 360 352 326

3542 352 333 384 403 362 355

3543 303 304 332 363 347 310

3544 280 292 315 327 341 304

3545 305 312 316 359 341 326

3546 312 343 355 3 74 359 345

3547 292 294 317 356 324 324

3548 TLL 353

3549 319 340 351 376 363 329

3550xNNMD

3551 348 350 358 373 366 337

MEAN 318 323 341 365 350 329

S.D. 21.7 21.6 21.2 21.3 16.1 18.6

N 21 20 20 20 20 20

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:32

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APPENDIX K

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

4526 320 315 333 341 336 292

4527 330 340 366 390 377 348

4528 315 328 323 340 333 336

4529 317 341 328 353 352 324

4530x NP

4531 267 292 306 330 316 318

4532 298 317 311 324 339 321

4533 322 337 358 388 351 359

4534 312 355 355 372 359 330

4535 271 273 292 302 300 277

4536 291 293 315 344 324 321

4537 330 352 366 373 352 361

4538 293 302 329 317 312 291

4539x NP

4540 293 290 306 333 300 304

4541xNNMD

4542 310 332 332 343 331 314

4543 318 321 312 376 351 350

4544 299 321 333 342 334 303

4545 298 322 341 328 365 322

4546 308 325 355 377 363 333

4547 PNMD 295 320 335 347 361 325

4548 323 336 348 364 360 349

4549 DIED

4550 339 360 368 380 381 359

4551 296 287 319 337 322 317

MEAN 307 321 333 350 342 325

S.D. 18.2 23.4 21.7 24.3 23.2 23.2

N 22 22 22 22 22 22

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN23

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- 2009 18:34

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APPENDIX L

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

1526 21

- 27 45

- 18 1 23

1527 DPP

1528 19 14 15

- 5

- 32 12

1529 PNMD a 12 16 7

- 40

1530 6 27 11

- 4

- 24 15

1531

- 3 14 23

- 30

- 22

- 19

1532x NP

1533 14 18 24

- 22 5 39

1534 2 27 16

- 8

- 36 1

1535x NP

1536 19 10 20 16

- 51 13

1537 17 23 24

- 24

- 24 16

1538

- 6 31 19

- 14

- 28 1

1539 6 23 12

- 17

- 24

- 2

1540 10 17 9

- 12

- 30

- 6

1541x NP

1542 1 26 14

- 17

- 18 6

1543 11 11 20

- 15

- 38

- 11

1544 26 35 5

- 28

- 39

- 1

1545 3 16 13

- 25

- 10

- 2

1546

- 9 34 29

- 32

- 10 11

1547

- 19 33 26

- 20

- 17 4

1548xNNMD

1549

- 5 33 27

- 15

- 24 17

1550xNNMD

1551 20

- 10 37

- 28

- 42

- 22

MEAN 7 18 20

- 15

- 25 5

S.D. 11.9 15.3 9.7 12.3 14.3 14.6

N 19 20 20 20 20 19

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

x=EXCLUDED FROM MEAN

a Body weight was not taken on Lactation Day 1 due to technician error.23

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- 2009 18:34

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- 4206 PAGE 304

APPENDIX L

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

2526 4 5 45

- 5

- 20 29

2527xNNMD

2528 6 16 25 4

- 37 13

2529 7 20 9 4

- 8 30

2530 18 22 15

- 9

- 54

- 8

2531 20 9 14

- 6

- 26 12

2532x NP

2 533

- 12

- 25 74

- 7

- 20 10

2534

- 1 29 6 7

- 25 16

2535 15 14 5

- 4

- 7 24

2536 20

- 6 15

- 7

- 22 0

2537 16

- 10 32

- 11

- 31

- 4

2538xNNMD

2539 6 9 23

- 17 7 28

2540 10 12 11 11

- 26 18

2541

- 16

- 31 56

- 12 6 4

2542xNNMD

2543 11 36

- 7

- 25

- 5 9

2544 12 8 6

- 11

- 7 7

2545 14 17

- 6

- 2

- 17 7

2546 5

- 4 46

- 17

- 30 0

2547 28 11 17 6

- 38 25

2548 14 6 15

- 7

- 9 20

2549 TLL

2550

- 1 16 12 6

- 30 3

2551 9 2 21 11

- 46

- 3

MEAN 9 7 21

- 4

- 21 11

S.D. 10.4 15.9 20.0 9.7 15.8 11.7

N 21 21 21 21 21 21

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:34

Huntingdon Life Sciences 00

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APPENDIX L

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RAT S

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

3526x NP

3527

- 1 31 16

- 27

- 10 8

3528 21 2 18 3

- 31 14

3529 12 16 25

- 10

- 40 3

3530 12 23 9

- 40 2 6

3531 3 22 24

- 24

- 24 1

3532 17 21 25

- 20

- 19 23

3533 37

- 15 46

- 10

- 22 36

3534

- 7 19 41

- 30

- 30

- 8

3535 4 11 17

- 3

- 10 19

3536 TLL

3537 5 33 19

- 6

- 21 32

3538xNNMD

3539xNNMD

3540

- 18 25 10 7

- 21 4

3541 1 11 23

- 8

- 26 2

3542

- 19 51 19

- 41 -7 4

3543 1 28 32

- 17

- 37 8

3544 12 23 12 14

- 37 24

3545 7 4 44

- 18

- 15 21

3546 31 12 19

- 14

- 15 33

3547 2 24 39

- 33 1 32

3548 TLL

3549 21 11 25

- 13

- 34 10

3550xNNMD

3551 2 8 16

- 7

- 29

- 11

MEAN 7 18 24

- 15

- 21 13

S.D. 14.1 13.7 11.1 14.8 12.2 13.8

N 20 20 20 20 20 20

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITT ER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:34

Huntingdon Life Sciences 00

- 4206 PAGE 306

APPENDIX L

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALA TION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

4526

- 5 18 8

- 5

- 44

- 28

4527 10 26 24

- 13

- 30 18

4528 13

- 5 17

- 7 3 21

4529 25

- 13 25

- 1

- 28 7

4530x NP

4531 24 15 23

- 14 3 51

4532 19

- 6 13 15

- 19 23

4533 15 21 30

- 36 7 37

4534 43 1 17

- 13

- 29 18

4535 2 19 10

- 2

- 23 6

4536 2 22 29

- 20

- 3 30

4537 22 13 7

- 21 9 31

4538 9 27

- 13

- 4

- 21

- 3

4539x NP

4540

- 3 15 27

- 33 4 11

4541xNNMD

4542 22 0 11

- 12

- 17 5

4543 3

- 9 64

- 25

- 1 32

4544 22 12 9

- 8

- 31 4

4545 25 19

- 13 37

- 43 24

4546 17 29 23

- 15

- 30 25

4547 PNMD 25 14 13 13

- 35 30

4548 14 12 16

- 4

- 11 26

4549 DIED

4550 21 8 13 1

- 22 21

4551

- 9 32 18

- 15

- 5 21

MEAN 14 12 17

- 8

- 17 19

S.D. 12.4 12.9 15.4 16.0 16.5 16.2

N 22 22 22 22 22 22

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

1026 135 109 96 89 80 71 71 66 62 61 60 53 50 50 50

1027 150 117 101 87 76 67 59 62 57 56 56 51 47 44 47

1028 149 121 92 94 80 71 70 68 62 61 58 55 51 50 48

1029 144 112 100 89 73 69 65 63 59 58 56 49 45 43 45

1030 151 117 102 92 76 75 69 65 59 59 54 48 46 44 46

1031 145 114 101 90 74 66 64 60 60 56 56 45 44 48 47

1032 148 114 103 86 81 76 67 64 61 61 59 48 45 46 46

1033 153 116 104 93 78 71 65 62 59 57 54 49 48 47 49

1034 148 119 104 91 78 72 63 60 59 60 53 49 49 44 43

1035 144 106 95 85 75 74 66 57 61 62 58 52 54 52 50

1036 152 113 101 94 79 75 63 61 62 58 50 48 47 44 45

1037 162 122 109 98 89 79 73 64 64 60 55 51 53 49 50

1038 160 127 105 94 81 72 68 65 59 55 49 46 47 43 44

1039 157 120 104 89 81 77 70 65 62 63 59 54 50 46 46

1040 165 121 10 6 94 83 74 68 66 61 57 54 51 53 49 47

1041 154 116 100 89 80 73 64 62 63 60 58 54 51 48 47

1042 153 1 15 101 93 73 70 67 63 55 55 52 51 48 48 45

1043 142 115 100 88 75 74 67 65 61 61 57 52 51 49 48

1044 155 118 106 95 79 77 71 65 63 61 57 52 51 50 48

1045 163 118 106 100 85 84 77 75 74 74 68 62 59 62 57

1046 162 121 104 92 79 75 67 64 62 62 58 50 49 46 46

1047 153 120 107 93 79 72 67 60 63 63 57 53 47 46 48

1048 168 121 108 97 79 74 65 65 66 60 52 50 54 52 47

1049 164 126 114 103 87 82 78 65 64 65 63 58 56 53 51

1050 162 126 109 95 82 77 73 65 62 62 56 51 50 47 44

1051 154 112 98 86 74 71 66 60 59 57 53 47 46 43 4 5

MEAN 153 117 103 92 79 74 68 64 62 60 56 51 50 48 47

S.D. 8.1 5.1 4.8 4.4 4.1 4.1 4.3 3.4 3.5 3.8 3.9 3.6 3.6 4.2 2.9

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE -B ODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

2026 141 109 94 83 79 72 66 60 59 59 53 52 51 48 49

2027 145 111 98 92 78 72 68 62 58 58 54 49 48 49 46

2028 154 111 94 89 78 68 63 64 58 51 54 48 47 47 46

2029 151 118 106 95 80 74 69 64 63 64 56 51 48 47 47

2030 147 111 99 88 77 71 67 62 57 55 53 48 48 46 42

2031 141 110 96 85 74 67 63 58 57 56 54 46 46 47 44

2032 159 114 100 87 77 69 68 61 59 58 56 51 49 47 42

2033 151 117 105 88 76 69 65 5 8 59 57 54 48 47 49 46

2034 143 115 99 87 76 74 68 62 63 61 55 51 50 51 48

2035 163 117 102 89 82 72 66 63 58 58 54 49 47 51 51

2036 148 107 95 81 71 67 63 60 59 59 55 46 46 49 46

2037 156 117 100 90 72 70 65 61 55 58 51 47 44 46 43

2038 151 114 96 83 78 70 65 64 61 59 52 47 46 45 43

2039 155 117 102 91 82 74 69 66 65 61 57 51 48 47 45

2040 164 125 106 99 85 77 68 69 66 63 61 56 54 49 48

2041 144 108 85 85 73 68 60 58 58 58 47 46 49 44 40

2042 159 120 100 92 78 69 62 60 55 55 52 47 46 44 40

2043 166 119 103 91 75 71 66 66 60 58 55 49 49 49 47

2044 166 122 103 90 74 72 67 66 62 62 60 54 52 52 47

2045 151 114 94 86 77 71 64 62 59 57 51 49 49 49 45

2046 171 122 106 93 84 75 70 64 60 61 59 54 54 54 52

2047 164 117 98 87 75 72 68 62 60 59 55 48 46 46 47

2048 156 110 105 93 81 76 71 64 58 61 56 48 49 49 46

2 049 154 113 93 85 78 71 66 65 59 58 57 50 50 48 47

2050 157 126 107 89 84 75 69 67 59 57 55 48 48 44 44

2051 169 120 104 88 81 72 67 63 61 59 57 52 47 45 43

MEAN 155 115 100 89 78 72 66 63 60 58 55 49 48 48 45

S.D. 8.7 5.1 5.3 4.0 3.7 2.7 2.7 2.7 2.6 2.7 3.0 2.6 2.4 2.6 3.0

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

3026 152 118 104 89 81 71 65 65 62 61 55 52 5 1 50 48

3027 143 113 96 82 72 67 63 57 55 56 51 46 44 46 47

3028 132 114 98 83 73 67 62 58 58 58 53 46 44 45 45

3029 148 116 97 88 76 70 64 59 59 57 50 48 46 46 46

3030 148 116 99 90 79 72 66 59 55 55 50 49 46 45 46

3031 157 123 101 90 79 75 70 65 62 61 55 53 49 52 52

3032 153 116 100 90 76 71 67 63 61 59 55 54 51 51 50

3033 149 118 97 91 82 74 68 63 60 58 54 51 48 49 49

3034 158 118 100 92 79 71 67 61 60 62 56 49 48 49 49

3035 137 105 95 81 73 70 64 58 59 59 54 51 48 49 52

3036 149 115 99 89 80 70 65 63 59 60 54 48 47 46 45

3037 145 114 100 86 82 73 65 64 61 62 54 51 48 52 52

3038 148 109 94 84 74 66 63 63 59 58 54 48 46 44 44

3039 158 126 100 88 91 82 73 69 63 62 53 51 49 48 43

3040 148 114 99 88 8 1 71 64 62 60 59 53 51 48 45 44

3041 149 118 95 84 72 70 64 59 59 63 54 50 46 46 47

3042 146 114 99 89 85 73 67 65 61 59 52 50 49 48 46

3043 150 119 101 87 73 69 65 66 62 60 55 52 50 48 48

3044 155 117 100 87 72 70 66 63 59 61 56 51 48 45 48

3045 144 117 97 90 78 70 65 63 58 60 56 54 52 47 50

3046 154 118 96 93 82 77 69 62 64 61 53 53 51 48 48

3047 141 113 96 86 72 67 66 63 60 60 54 48 45 44 45

3048 158 117 105 96 83 72 70 68 64 64 60 53 51 50 47

3049 143 110 96 86 79 68 67 64 60 60 57 55 51 50 48

3050 153 119 107 93 81 75 70 65 62 61 56 51 48 48 48

3051 161 125 108 97 85 74 67 66 59 60 55 49 46 45 41

MEAN 149 116 99 88 79 71 66 63 60 60 54 51 48 47 47

S.D. 6.9 4.5 3.6 3.9 5.0 3.6 2.5 3.0 2.3 2.0 2.2 2.3 2.2 2.3 2.7

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2623

- JUN

- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP IV 20000 MG/ M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

4026 140 110 91 81 73 66 58 56 58 57 55 47 47 48 48

4027 129 97 90 82 74 68 63 62 62 60 57 50 47 52 54

4028 162 127 106 95 88 80 76 70 69 65 63 58 57 55 53

4029 151 112 96 87 79 72 66 62 59 58 54 53 50 45 44

4030 141 109 97 89 77 71 65 61 59 57 51 48 45 46 46

4031 150 116 98 90 81 70 68 63 60 60 55 51 48 50 50

4032 150 122 102 92 77 70 65 62 59 59 53 49 52 48 48

4033 150 114 94 88 77 68 65 60 60 6 0 56 51 42 45 49

4034 150 113 96 84 75 71 63 57 59 59 51 52 52 48 49

4035 141 112 103 93 79 73 68 63 58 55 51 50 49 47 43

4052 IW 116 101 92 82 76 72 67 58 63 57 55 49 52 49

4037 141 108 89 81 73 66 64 61 59 56 54 47 45 52 51

4038 144 112 95 88 77 70 68 65 64 61 54 50 54 52 53

4039 156 121 104 93 79 72 67 64 59 57 54 52 53 50 48

4040 158 114 99 96 85 78 72 66 64 61 57 52 56 52 52

4041 144 112 98 90 81 74 63 59 60 58 51 50 49 46 45

4042 139 107 95 90 80 74 70 66 64 64 60 56 51 50 53

4043 147 114 101 97 86 84 70 65 67 65 58 58 55 56 53

4044 152 111 99 91 81 76 68 64 63 61 57 51 47 48 47

4045 154 116 99 93 82 76 69 64 61 60 7 52 51 50 48

4046 162 120 103 99 77 72 69 62 63 59 54 47 48 47 47

4047 150 12 0 108 103 90 82 76 69 66 64 57 57 58 56 56

4048 152 119 99 95 85 71 67 63 60 58 53 53 51 53 51

4049 1 47 115 98 88 79 74 67 64 65 62 53 57 56 55 54

4050 150 117 101 93 80 78 65 62 63 63 62 57 52 52 52

4051 150 114 99 89 80 75 70 63 61 60 53 52 51 48 46

MEAN 148 114 98 91 80 73 68 63 62 60 53 52 51 50 50

S.D. 7.5 5.7 4.6 5.2 4.3 4.5 4.1 3.2 2.9 2.8 10.0 3.3 4.0 3.3 3.4

N 25 26 26 26 26 26 26 26 26 26 26 26 26 26 26

IW=Invalid Weight: Feed le ft greater than feed fed weight23

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- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

1526 126 98 91 85 80 76 68 68 67 59 55

1527 125 94 95 87 81 78 69 72 70 64 62

1528 134 95 93 89 78 74 70 71 68 63 61

1529 133 96 96 83 78 76 71 74 69 65 67 72 58

1530 131 93 92 86 81 82 74 76 70 73 62

1531 121 99 92 91 81 75 66 70 65 72 70

1532 119 93 83 80 75 70 66 67 63 63 62

1533 133 103 94 93 86 81 72 73 72 69 62

1534 SF 102 94 91 83 74 74 74 72 69 SF

1535 120 94 91 86 72 76 74 72 65 70 62

1536 125 98 91 92 87 83 77 76 73 69 65

1537 133 108 98 97 90 85 78 7 8 67 73 65

1538 116 91 88 84 74 74 65 66 65 64 61

1539 122 95 92 82 81 78 72 67 65 61 63

1540 139 100 98 90 80 78 77 70 66 64 64

1541 128 96 100 90 81 83 74 72 71 73 61

1542 127 103 96 88 83 76 73 69 65 62 63

1543 143 110 102 89 82 76 71 70 70 68 68

1544 130 101 86 89 87 78 68 68 65 59 60

1545 111 86 93 76 74 70 65 66 62 63 62

1546 109 89 85 85 75 69 70 72 63 63 64

1547 134 108 97 100 85 88 76 77 71 71 68

1548 119 100 94 87 84 79 75 71 70 69 59 49 55 59

1549 130 108 99 89 84 87 77 76 65 68 63

1550 135 96 96 87 77 73 65 70 65 63 67 57 58 55

1551 130 94 98 86 75 74 63 70 63 63 56

MEAN 127 98 94 88 80 78 71 71 67 66 63 59 57 57

S.D. 8.3 6.1 4.5 4.9 4.6 5.0 4.4 3.5 3.3 4.4 3.6 11.2 2.0 2.6

N 25 26 26 26 26 26 26 26 26 26 25 3 3 2

SF=Spilled Feeder23

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- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF S TUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

2526 133 104 93 92 81 79 76 78 71 68 67

2527 123 100 98 89 83 79 77 74 70 70 68 63 62 6 0

2528 121 97 90 88 76 76 72 68 64 66 62

2529 118 94 85 89 83 74 65 61 64 57 52

2530 124 98 91 83 80 79 74 70 70 62 69

2531 129 109 99 93 91 82 76 73 71 79 80

2532 128 97 90 91 84 79 78 70 69 78 64

2533 128 97 91 92 82 78 74 71 69 67 66

2534 129 100 98 92 91 85 85 79 69 70 67

2535 127 109 100 91 85 83 73 73 73 86 64

2536 140 110 100 90 88 83 79 76 66 71 62

2537 129 102 95 96 89 87 82 75 74 70 66

2538 132 94 90 82 76 74 69 65 70 61 53 59 49 46

2539 135 106 104 95 85 85 85 77 73 72 77

2540 136 105 110 98 96 90 76 85 71 85 SF

2541 133 101 95 90 86 7 8 70 68 62 62 59

2542 128 96 90 80 83 80 73 65 60 61 61 56 56 54

2543 127 104 105 93 87 86 83 75 68 64 62

2544 130 94 101 105 96 97 76 75 71 70 66

2545 125 99 95 87 80 79 70 75 72 69 70

2546 146 100 103 96 90 85 84 71 70 74 73

2547 122 94 81 90 81 73 70 64 63 66 59

2548 134 105 99 93 90 83 77 73 71 74 76

2549 129 102 94 98 88 82 80 70 71 71 58 55 a 49

2550 126 101 92 82 78 75 68 67 66 65 61

2551 138 97 95 95 84 77 73 64 61 56 54

MEAN 130 101 95 91 85 81 76 72 68 69 65 59 56 54 49

S.D. 6.2 4.8 6.5 5.6 5.4 5.4 5.4 5.4 3.8 7.5 7.1 3.5 6.7 5.7 0.0

N 26 26 26 26 26 26 26 26 26 26 25 3 3 4 1

SF=Spilled Feeder

a Animal was pregnant but had total litter loss. Recording of food consumption was resumed.23

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRA MS/KG/DAY)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

3526 123 93 88 85 74 70 70 68 67 62 51

3527 12 6 95 89 85 76 74 70 72 64 64 56

3528 123 98 91 85 75 78 72 76 69 78 50

3529 117 88 85 82 76 72 70 67 67 65 48

3530 138 105 91 95 85 81 77 77 64 69 57

3531 150 101 93 87 79 74 68 68 64 62 54

3532 121 91 88 82 75 76 71 69 61 67 51

3533 120 97 87 86 75 72 65 59 57 59 41

3534 122 103 93 92 84 79 77 76 65 67 55

3535 131 100 96 90 87 76 75 69 69 70 68

3536 130 92 92 81 76 76 76 71 62 66 64 61 a 56

3537 120 96 97 83 78 76 74 67 67 74 73

3538 130 102 96 91 84 79 73 72 66 74 71 57 54 52

3539 125 96 88 88 87 77 72 71 68 67 63 55 46 51

3540 154 120 111 102 94 93 92 86 77 74 80

3541 140 104 102 90 84 82 74 75 74 84 60

3542 SF 105 103 90 93 83 85 72 67 74 66

3543 127 97 91 87 81 80 71 66 68 77 61

3544 128 103 95 91 87 80 77 75 76 71 71

3545 130 103 93 92 84 84 70 74 64 72 66

3546 142 112 110 102 86 83 77 73 73 74 80

3547 128 100 92 94 80 82 81 75 69 74 65

3548 136 102 93 92 90 77 73 69 68 77 53 51 a 45

3549 114 93 90 87 104 82 83 71 70 67 65

3550 126 109 104 84 79 80 76 70 70 71 74 55 53 51

3551 138 105 93 90 79 77 72 72 70 65 65

MEAN 130 100 94 89 83 79 75 72 67 70 62 56 51 53 51

S.D. 9.7 7.0 6.7 5.4 7.2 4.7 5.7 4.8 4.4 5.9 9.8 0.9 4.5 4.4 7.8

N 25 26 26 26 26 26 26 26 26 26 26 3 3 5 2

SF=Spilled Feeder

a Animal was pregnant but had total litter loss. Recording of foo d consumption was resumed.23

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- 2009 18:38

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APPENDIX M

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN R ATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 4 9 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

4526 122 93 93 88 84 80 76 73 66 63 58

4527 132 SF 94 86 85 80 75 73 66 61 65

4528 145 100 98 90 87 84 76 72 66 69 64

4529 128 90 89 81 80 78 74 66 64 65 60

4530 124 83 91 82 86 80 78 78 67 66 65

4531 123 95 88 81 74 76 74 65 63 60 59

4532 131 96 99 101 90 91 88 75 70 74 73

4533 129 90 90 91 87 86 81 76 75 77 67

4534 116 85 84 84 81 78 73 71 65 68 62

4535 135 92 90 85 82 79 78 74 66 63 66

4536 119 88 91 85 79 79 76 70 67 68 69

4537 125 93 93 85 86 79 78 76 71 74 72

4538 130 98 92 87 84 78 77 71 71 65 62

4539 135 102 93 92 82 88 91 75 68 76 58

4 540 135 91 88 89 80 86 83 77 69 67 67

4541 127 97 88 85 85 77 73 68 68 65 60 60 53 58

4542 149 111 104 94 91 85 80 77 68 71 69

4543 144 101 92 89 82 73 77 66 68 64 64

4544 145 101 99 91 85 80 76 70 66 69 68

4545 126 96 86 86 81 72 72 69 65 64 61

4546 131 101 97 88 84 80 82 70 70 74 75

4547 137 102 98 90 84 86 74 62 68 64 65 60 b

4548 136 102 89 98 86 78 76 75 68 67 65

4549 132 104 91 90 79 84

4550 134 100 94 87 82 76 73 65 65 66 62

4551 154 112 98 96 92 89 79 77 75 71 69

MEAN 133 97 93 89 84 81 78 72 68 68 65 60 53 58

S.D. 9.2 7.1 4.8 4.9 4.0 4.8 4.6 4.4 2.8 4.7 4.6 0.4 0.0 0.0

N 26 25 26 26 26 26 25 25 25 25 25 2 1 1

SF=Spilled Feeder

b Food consumption continued until the last day before delivery because this animal had no Gestation Day 0 (but the animal was found to

be pregnant).23

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APPENDIX N

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

1526 88 76 68 60 73

1527 DPP 65 74 67 58 66

1528 74 75 69 57 69

1529xPNMD

1530 65 73 68 67 68

1531 64 66 63 56 62

1532x NP 62 68 66 64 65

1 533 72 74 69 60 69

1534 73 77 76 70 74

1535x NP 80 76 60 72

1536 80 80 74 52 71

1537 69 82 76 68 74

1538 67 68 65 59 65

1539 75 71 70 60 69

1540 79 76 72 65 73

1541x NP 77 74 69 54 69

1542 83 71 64 59 69

1543 71 72 69 76 72

1544 73 79 74 57 71

1545 71 74 67 61 68

1546 77 77 75 63 73

1547 80 81 73 66 75

1548xNNMD

1549 78 75 71 65 72

1550xNNMD

1551 72 67 66 63 67

MEAN 74 74 70 62 70

S.D. 6.3 4.3 3.9 5.7 3.4

N 20 20 20 20 20

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

x=EXCLUDED FROM MEAN

SF=Spilled Feeder23

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APPENDIX N

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

2526 77 80 70 63 72

2527xNNMD

2528 70 58 69 64 65

2529 66 68 67 56 64

2530 70 73 72 62 69

2 531 77 78 71 60 72

2532x NP 61 65 63 63 63

2533 70 72 69 64 69

2534 75 80 73 64 73

2535 73 75 71 56 69

2536 77 80 75 66 75

2537 85 89 84 70 82

2538xNNMD

2539 76 84 78 59 74

2540 92 98 82 72 86

2541 69 74 68 57 67

2542xNNMD

2543 61 68 56 61 61

2544 79 76 67 62 71

2545 77 88 71 68 76

2546 88 94 79 70 83

2547 62 59 66 57 61

2548 70 68 70 66 68

2549 TLL 76 76 68 63 71

2550 72 69 69 62 6 8

2551 70 75 71 47 66

MEAN 74 76 71 6 2 71

S.D. 7.6 10.0 6.0 5.7 6.5

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

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APPENDIX N

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

3526x NP 59 62 57 57 59

3527 8 6 76 68 57 72

3528 75 73 71 63 71

3529 70 75 70 65 70

3530 78 77 73 60 72

3 531 72 80 78 68 74

3532 72 74 70 67 71

3533 65 63 61 58 62

3534 81 82 73 68 76

3535 66 65 65 55 63

3536 TLL 71 71 65 16 56

3537 73 70 69 61 68

3538xNNMD

3539xNNMD

3540 90 78 47 72

3541 80 80 71 65 74

3542 73 76 72 67 72

3543 73 74 71 66 71

3544 77 75 71 65 72

3545 68 71 68 64 68

3546 74 74 72 64 71

3547 82 81 78 67 77

3548 TLL 73 79 68 62 71

3549 74 87 75 64 75

3550xNNMD

3551 75 76 71 63 71

MEAN 74 76 71 6 1 70

S.D. 5.3 6.3 4.2 11.1 4.9

N 21 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

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- 2009 18:45

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APPENDIX N

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7 - 14 14 - 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

4526 71 69 71 66 69

4527 7 2 69 67 62 67

4528 73 72 72 63 70

4529 68 74 67 61 67

4530x NP 77 75 72 42 67

4 531 64 65 63 61 63

4532 78 86 81 65 78

4533 79 88 83 70 80

4534 74 75 71 67 72

4535 71 75 71 68 71

4536 71 74 74 63 70

4537 89 77 75 70 78

4538 72 75 68 60 69

4539x NP 77 78 70 50 69

4540 85 87 71 62 76

4541xNNMD

4542 74 80 80 70 76

4543 68 71 68 62 67

4544 78 80 73 67 74

4545 73 73 73 69 72

4546 87 76 77 70 77

4547xPNMD

4548 67 74 35 64 60

4549 DIED

4550 69 73 71 63 6 9

4551 71 85 77 73 76

MEAN 74 76 71 6 5 72

S.D. 6.5 6.2 9.6 3.7 5.1

N 21 21 21 21 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEA N23

- JUN

- 2009 18:46

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APPENDIX O

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

1526 107 87 148 184 328 171

1527 DPP

1528 105 114 139 149 256 153

1529 PNM D a 98 137 174 256 166

1530 99 100 150 167 312 166

1531 105 93 138 168 304 161

1532x NP

1533 113 109 146 169 292 166

1534 111 122 140 197 343 183

1535x NP

1536 101 115 146 152 213 146

1537 103 127 154 176 308 174

1538 83 115 136 183 316 166

1539 113 121 141 174 316 173

1540 123 125 144 170 351 183

1541x NP

1542 94 106 134 164 302 160

1543 115 122 158 210 373 196

1544 97 119 143 155 306 164

1545 94 100 129 156 271 150

1546 106 126 147 163 298 168

1547 125 155 149 176 348 191

1549 110 123 150 177 323 177

1551 109 126 156 177 323 178

MEAN 106 115 144 172 307 169

S.D. 10.3 15.3 7.7 14.7 37.0 12.8

N 19 20 20 20 20 20

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE x=EXCLUDED FROM MEAN

a Feed consumption data was not collected on Lactation Day 1 due to technician error.23

- JUN

- 2009 18:46

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- 4206 PAGE 320

APPENDIX O

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

2526 102 126 1 48 176 307 172

2528 59 128 144 168 316 163

2529 82 11 1 CF 180 324 174

2530 102 116 125 168 269 156

2531 74 99 131 170 307 156

2532x NP

2533 86 110 141 175 309 164

2534 90 106 104 140 221 132

25 35 104 101 99 154 276 147

2536 90 102 133 165 303 159

2537 113 87 121 190 279 158

2539 99 101 136 179 283 160

2540 93 132 147 208 388 194

2541 74 85 125 165 325 155

2543 108 108 112 174 339 168

2544 64 73 86 101 185 102

2545 119 127 124 164 308 168

2546 123 133 147 195 368 193

2547 105 120 134 194 333 177

2548 71 91 139 189 258 150

2549 TLL

2550 95 110 129 186 274 159

2551 80 99 133 203 297 162

MEAN 92 108 128 174 299 160

S.D. 17.6 16.3 16.8 23.3 45.1 19.5

N 21 21 20 21 21 21

NP=NOT PREGNANT TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN

CF=Contaminated Feeder23

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- 2009 18:46

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APPENDIX O

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY ST UDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

3526x NP

3527 110 123 137 175 322 173

3528 60 77 106 129 240 122

3529 97 106 139 162 299 161

3530 99 106 126 162 325 164

3531 103 114 135 190 331 175

3532 108 108 133 156 279 157

3533 91 96 137 174 316 163

3534 122 120 149 178 315 177

3535 97 110 131 125 308 154

3536 TLL

3537 101 121 135 166 259 156

3540 115 113 129 171 313 168

3541 118 115 147 168 290 168

3542 82 140 140 180 313 171

3543 100 121 146 162 293 164

3544 122 124 150 185 316 179

3545 136 134 161 170 278 176

3546 115 123 145 181 331 179

3547 74 105 153 176 303 162

3548 TLL

3549 107 107 134 131 333 162

3551 86 94 113 156 265 143

MEAN 102 113 137 165 301 164

S.D. 17.9 14.1 13.0 18.2 26.1 13.5

N 20 20 20 20 20 20

NP=NOT PREGNANT TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN23

- JUN

- 2009 18:46

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- 4206 PAGE 322

APPENDIX O

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7 - 14 14 - 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

4526 90 108 140 171 262 154

4527 95 114 133 150 275 154

4528 74 80 121 150 287 142

4529 78 87 136 154 311 153

4530x NP

4531 91 109 132 168 266 154

4532 84 103 145 182 329 169

4533 124 112 141 177 323 175

4534 104 117 138 155 243 151

4535 88 103 124 173 314 160

4536 105 112 141 182 330 174

4537 116 112 136 1 79 296 168

4538 119 124 143 187 312 177

4539x NP

4540 102 112 131 169 317 166

4542 79 94 131 171 326 160

4543 108 114 145 174 288 166

4544 117 116 134 184 310 172

4545 103 120 141 155 253 155

4546 114 124 146 185 266 167

4547 PNMD 99 98 134 163 312 161

454 8 102 109 139 183 262 159

4550 103 114 127 185 284 163

4551 106 123 139 169 239 156

MEAN 100 109 136 171 291 162

S.D. 14.0 11.4 6.8 12.0 29.0 9.0

N 22 22 22 22 22 22

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE x=EXCLUDED FROM MEAN08

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 323

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

1526 /E D D D /E D D D /E D D D /E M D D /E D D D /P 14 1 5 1 5 4.0

1527 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

1528 D /E M D D /E D D D /E M D D /E D D D D D D D 15 0 4 2 3 4.0

1529 D /E E D D D /E E E D D D D /E M D D D /E E D 12 0 8 1 3 5.7

1530 /E D D D /E M D D D D D D D D D D D D D D /E 17 0 3 1 1 4.0 +

1531 D /E M D D D /E M D D D D D D D D D D D D D 17 0 2 2 1 5.0 +

1532 D D /E D D D /E D D D /E D D /E E M D D /E M D 13 0 6 2 4 4.0

1533 D D D /E D D D /E M D D /E M D D /E E M D D /P 12 1 5 3 4 4.3

1534 D /E D D D /E D D D /E M D D /E M D D /E M D D 13 0 5 3 4 4.0

1535 /P E D D D /E E D D D D /P D D /E M D D D /E D 13 2 5 1 4 4.8

1536 /E D D D /E D D D /E M D D /E M D D /P P D D D 13 2 4 2 4 4.0

1537 D /E D D D /E D D D /E M D D /E D D D /E E D D 14 0 6 1 4 4.0

1538 D D D /E D D D /E D D D /E E M D D D D /E M D 1 4 0 5 2 3 5.0

1539 /E D D D /E D D D /E M D D D /E M D D D /E M D 13 0 5 3 4 4.5

1540 D D /E D D D /E D D D /E D D D /E D D D /E M D 15 0 5 1 4 4.0

1541 D D D /E M D D /E D D D /E D D D /E M D D /E D 14 0 5 2 4 4.0

1542 D D /E M D D /E D D D /E M D D /E D D D /E M D 13 0 5 3 4 4.0

1543 D D /E D D D /E D /E D /E D D D /E D D D /E D D 15 0 6 0 5 3.2

1544 /E D D D /E M D D D D D /E E M D D /E M D D /E 12 0 6 3 4 5.0

1545 D /E D D D /E D D D /E E D D D /E D D D /E D D 15 0 6 0 4 4.3

1546 D /E M D D /E D D D /E D D D /E D D D /E D D D 15 0 5 1 4 4.0

1547 D /P E D D D /E D /E D /E D D D /E M D D /E D D 13 1 6 1 5 3.4

1548 D D D /E M D D D /E M D D /E M D D /E M D D /E 12 0 5 4 4 4.3

1549 D /E D D D /E M D D /E M D D /E M D D /E E D D 12 0 6 3 4 4.0

1550 D D /E D D D /E D D D /E M D D /E D D D /E D D 15 0 5 1 4 4.0

1551 D D /P E M D D D /E M D D /E E M D D D /E M D 11 1 5 4 3 5.3

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+ Period of pseudopregnancy excluded.08

- SEP

- 2009 20:29

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- 4206 PAGE 324

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

2526 D /E D D D /E M D D /E D D D /E D D D /E M D D 14 0 5 2 4 4.0

2527 D D D D D D /E D D D /E D D D /E D D D /E M D 16 0 4 1 3 4.0

2528 D D D D D D /E M D D /E D D D /E D D D /P E M 14 1 4 2 3 4.0

2529 /E D D D /E D D D /E D D D /E M D D /E D D /E E 13 0 7 1 5 3.8

2530 D D D D /E D D D /P E D D D /E M D D D D /P D 15 2 3 1 3 5.0

2531 /E D D D /E D D D /E D D D D /E D D D D D D D 17 0 4 0 3 4.3

2532 D D D /P E M D D D D D D D D D D D D D D D 18 1 1 1

- - +

2533 D D D D D D /E D D D /E D D D /E D D D /E M D 16 0 4 1 3 4.0

2534 D D D D D D /E M D D /E M D D /E D D D /E D D 15 0 4 2 3 4 .0

2535 D D D /E D D D D D D D D D D D D D D D D D 20 0 1 0

- - +

2536 D D D D D D /E M D D /E D D D /E M D D /E E D 14 0 5 2 3 4.0

2537 D /E D D D /E M D D /E M D D /E D D D D /E E D 13 0 6 2 4 4.3

2538 D D D D D D D D D D D /E E M D D /E D D D /E 16 0 4 1 2 4.5 +

2539 D D D D D D /E D D D /P E D D D /E E D D D /P 15 2 4 0 3 4.7

2540 /E D D D /E M D D /E M D D /E M D D /E M D D /E 11 0 6 4 5 4.0

2541 D D D D D D /E E D D D /E M D D D D /E D D D 16 0 4 1 2 5.5

2542 D D D D D D /E D D D /E M D D /E M D D /E E D 14 0 5 2 3 4.0

2543 D /E D D /P E M D D /E D D D /E D D /P E E M D 11 2 6 2 4 3.8

2544 /M D D /E D D D /E M D D /E E M D D /E D D D /E 12 0 6 3 5 4.0

2545 D D D /E D D D /E D D D /E D D D /E D D D /E E 15 0 6 0 4 4.0

2546 D D D D D D /E D D D /E D D D /E D D D /E D D 17 0 4 0 3 4.0

2547 D D D /E D D D /E E D D /E D D D /E D D D /E D 15 0 6 0 4 4.0

2548 /E D D D /E D D D D D D D D D D D D D D D D 19 0 2 0 1 4.0 +

2549 /E E D D D D /E D D D /P E D D D D /E D D D /E 14 1 6 0 4 5.0

2550 D D D /E D D D /E D D D /E D D D / E D D D /P E 15 1 5 0 4 4.0

2551 D /E D D D /E D D D /E D D D /E D D D /E E D D 15 0 6 0 4 4.0

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+ Period of pseudopregnancy excluded.08

- SEP

- 200 9 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 325

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

3526 D D /E M D D /E E D D /E D D /E E D D D /E M D 12 0 7 2 4 4.0

3527 D D D D D /E D D D /E D D D /E M D D /E D D D 16 0 4 1 3 4.0

3528 D /E E D D D D D D D D D D D D D D D /E M D 17 0 3 1

- - +

3529 /E D D D /E M D D /E D D D /E D D D /E D D D /P 14 1 5 1 5 4.0

3530 D D /E D D D /P E M D D /P E D D D /E E M D D 12 2 5 2 3 4.7

3531 D /E D D D /E D D D /E D D D /E D D D /E D D D 16 0 5 0 4 4.0

3532 D D /E D D D D /E D D D /P M M D D /E E M D D 13 1 4 3 3 4.7

3 533 D D D D /P E M D D D D /E M D D D /E E M D D 13 1 4 3 2 6.0

3534 D /E M D D /E D D D /E M D D /E M D D /E M D D 12 0 5 4 4 4.0

3535 /E M D D /P E D D D /E E D D D /E E M D D D D 12 1 6 2 3 4.7

3536 D D /E M D D /E M D D /E D D D /E D D D /E M D 13 0 5 3 4 4.0

3537 D D D /E D D D /E E M D D D D D D D D D D D 17 0 3 1 1 4.0 +

3538 D /E E M D D /E E D D D D D D D D D D D D D 16 0 4 1 1 5.0 +

3539 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

3540 D /E D D D /E M D D /E M D D /E M D /E E D D D 12 0 6 3 4 3.8

3541 D D /E E D D D D D D D D D D /E D /E E D D D 16 0 5 0 1 2 .0 +

3542 /E M D D /E D D D D D D D D D /E D D D /E D D 16 0 4 1 3 6.0

3543 D D D /E D D D D D D D D D D D D D D /E D D 19 0 2 0

- - +

3544 /E D D /P E D D D /E D D /P E D D D /E D D D /E 13 2 6 0 5 4.0

3545 D D D /E D D D D D D D D D D D D /E E D D D 18 0 3 0

- - +

3546 /P E M D D /E D D D /P E D D D D D D D D D D 15 2 3 1 2 4.5 +

3547 D D D /E D D D /E D D D /E D D /E E D D D /E D 15 0 6 0 4 4.0

3548 /E D D D /E D D D D D D D D /E D D /E D D /E E 15 0 6 0 4 4.8

3549 D D D /E D D D /E D D D /E D D D /E E D D D /E 15 0 6 0 4 4.3

3550 D D /E D D D /E M D D /E D D D D D D D D D D 17 0 3 1 2 4.0 +

3551 D D D /P E D D D /E E D D D D /E D D D /E E D 14 1 6 0 3 5.0

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+ Period of pseudopregnancy excluded.08

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 326

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

4526 D /E M D D /E D D D /E M D D /E M D D /P E E D 11 1 6 3 4 4.0

4527 D D /E E M D D D /E E D D D /E E M D D D /E D 12 0 7 2 3 5.7

4528 D D D /E D D D /E D D D /E D D D /E D D D /P E 15 1 5 0 4 4.0

4529 D D D /E M D D /E M D D /E M D D /E M D D /E E 11 0 6 4 4 4.0

4530 D /E D D D /E D D D /E D D D /E M D D /E M D D 14 0 5 2 4 4.0

4531 D D /E D D D /E D D D /E D D D /E D D D /E E D 15 0 6 0 4 4.0

4532 D D /E D D D /E M D D /E D D D /E D D D /E D D 15 0 5 1 4 4.0

453 3 D D D /E D D D /E D D D /E M D /E E E E E D D 12 0 8 1 3 3.7

4534 D D D /E M D D /E M D D /E D D D /E D D D D /E 14 0 5 2 4 4.3

4535 D /E M D D /E D D /P E D D D /E D D /E E D D D 13 1 6 1 4 3.8

4536 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0

4537 /P E D D D /E E D D D /P E M D D /E E D D D D 12 2 6 1 3 5.0

4538 /E M D D /E D D D /E M D D /E D D D /E D D D /E 13 0 6 2 5 4.0

4539 D D D /E D D D /E D D D D D D D D D D D D D 19 0 2 0 1 4.0 +

4540 D D D /P E D D D D /E D D D /P E D D D /E D /E 14 2 5 0 4 4.3

4541 /E D D D /E D D D /E D D D /E D D D /E E E D D 14 0 7 0 4 4.0

4542 D /P E D D D /P E D D D D /E D D D /E E E D D 13 2 6 0 3 5.0

4543 /E M D D /E M D D /E M D D D /M D D D /E M D D 12 0 4 5 4 4.3

4544 /E M D D /E E M D D /E M D /E D /E D D D D /E E 10 0 8 3 5 3.8

4545 D /E M D D /E E D D D /E D D D /E D D D /E D D 14 0 6 1 4 4.3

4546 D D /E E D D /E D D D /E D D D /E M D D /E D D 14 0 6 1 4 4.0

4547 /E D D D /E D D D /E D D D /E D D D /E M D D /E 14 0 6 1 5 4.0

4548 D /E D D D /E D D D /P E D D /P E D D D /E D D 14 2 5 0 4 4.3

4550 D D /E D D D /E D D D /E M D D /E D /E D /E D D 14 0 6 1 5 3.2

4551 /E D D D /E D D D /E D D /E E D D D D D D D D 16 0 5 0 3 3.7

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

+ Period of pseudopregnancy excluded.08

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 327

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

1526 S

1527 D D D S

1528 D D D C

1529 D D D D D D D D D D D D D D

1530 D D D S

1531 C

1532 D E E C

1533 C

1534 C

1535 C

1536 E D D D P S

1537 D E C

1538 D S

1539 D C

1540 D C

1541 D D C

1542 D C

1543 D E E D D E S

1544 M D D C

1545 D C

1546 P C

1547 D C

1548 M D D D D D D D D D D D D D

1549 E C

1550 D E D D D E M D D E D D D E

1551 D P C

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG08

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 328

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

2526 E C

2527 D E D D D E D D D E D E E E

2528 D D S

2529 D D E S

2530 D D D D D D D D C

2531 D D C

2532 D D C

2533 D C

2534 D E C

2535 S

2536 D P E E C

2537 C

2538 M D D E D D D E E D D D D D

2539 E C

2540 E M D C

2541 E C

2542 D E D D E E E E E E E E E E

2543 D C

2544 M D C

2545 E E E C

2546 D C

2547 D D D S

2548 D D C

2549 E C

2550 C

2551 D E C

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG08

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 329

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATIN G DATA

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

3526 D S

3527 S

3528 D E E D D C

3529 C

3530 D C

3531 P S

3532 D C

3533 D P S

3534 D C

3535 D D D D D D D C

3536 D E S

3537 D D S

3538 D E M D D E M D D E D D D E

3539 E E D D D E D D D D D D D D

3540 E C

3541 S

3542 D C

3543 D S

3544 D D D S

3545 E S

3546 D D D D S

3547 D D S

3548 D D D E C

3549 D D D P C

3550 D D D D D D D D D D D D D D

3551 D C

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG08

- SEP

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 330

APPENDIX P

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVAL UATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

4526 D D P E D D D S

4527 D D P S

4528 E D D D C

4529 M D D P C

4530 E E M D C

4531 D D C

4532 D E D D D S

4533 S

4534 E E D D D C

4535 S

4536 D C

4537 E E D D D D C

4538 D C

4539 D S

4540 S

4541 D D E E E D E E E D E E E E

4542 D P C

4543 P S

4544 S

4545 D D E D D D D S

4546 D E C

4547 D D D D D D D D D D D D D D

4548 D P S

4550 D E M D D E M D D P C

4551 E C

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 331

APPENDIX Q

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Date of Date of Date of

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition

-----------------------------------------------------------------------------------------------------------------------------------

1526 1026 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

1527 1027 + DPP, P 28

- OCT

- 02 1

- NOV

- 02 23- NOV

- 02

1528 1028 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

1529 1029

- PNMD 28

- OCT

- 02 27- NOV

- 02

1530 1030 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

1531 1031 + P 28

- OCT

- 02 29

- OCT

- 02 20- NOV

- 02

1532 1032 + NP 28

- OCT

- 02 1

- NOV

- 02

1533 1033 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

1534 1034 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

1535 1035 + NP 28

- OCT

- 02 29

- OCT

- 02

1536 1036 + P 28

- OCT

- 02 3

- NOV

- 02 25

- NOV

- 02

1537 1037 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

1538 1038 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1539 1039 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1540 1040 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1541 1041 + NP 28

- OCT

- 02 31

- OCT

- 02

1542 104 2 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1543 1043 + P 28

- OCT

- 0 2 4

- NOV

- 02 26

- NOV

- 02

1544 104 4 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

1545 1045 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1546 104 6 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1547 1047 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1548 104 8

- NNMD 28

- OCT

- 02

1549 1049 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

1550 1050

- NNMD 28

- OCT

- 02

1551 1051 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE P=PREGNANT23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 332

APPENDIX Q

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Date of Date of Date of

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition

-----------------------------------------------------------------------------------------------------------------------------------

2526 2026 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

2527 2027

- NNMD 28

- OCT

- 02

2528 2028 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

2529 2029 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

2530 2030 + P 28

- OCT

- 02 6

- NOV

- 02 28

- NOV

- 02

2531 2031 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

2532 2032 + NP 28

- OCT

- 02 31

- OCT

- 02

2533 2033 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

2534 2034 + P 28

- OCT

- 02 31

- OCT

- 02 23

- NOV

- 02

2535 2035 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

2536 2036 + P 28

- OCT

- 02 2

- NOV

- 02 24

- NOV

- 02

2537 2037 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

2538 2038

- NNMD 28

- OCT

- 02

2539 2039 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

2540 2040 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

2541 2041 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

2542 2042

- NNMD 28

- OCT

- 02

2543 2043 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

2544 2044 + P 28

- OCT

- 02 31

- OCT

- 02 23

- NOV

- 02

2545 2045 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

2546 2046 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

2547 2047 + P 28

- OCT

- 02 1

- NOV

- 02 24

- NOV

- 02

2548 2048 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

2549 2049 + TLL,P 28

- OCT

- 02 30

- OCT

- 02 22

- NOV

- 02

2550 2050 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

2551 2051 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS P=PREGNA NT23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 333

APPENDIX Q

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXI CITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Da te of Date of Date of

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition

-----------------------------------------------------------------------------------------------------------------------------------

3526 3026 + NP 28

- OCT

- 02 30

- OCT

- 02

3527 3027 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

3528 3028 + P 28

- OCT

- 02 3

- NOV

- 02 24

- NOV

- 02

3529 3029 + P 28

- OCT

- 02 29

- OCT

- 02 19

- NOV

- 02

3530 3030 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3531 3031 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3532 3032 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3533 3033 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

3534 3034 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3535 3035 + P 28

- OCT

- 02 5

- NOV

- 02 27

- NOV

- 02

3536 3036 + TLL,P 28

- OCT

- 02 31

- OCT

- 02 24

- NOV

- 02

3537 3037 + P 28

- OCT

- 02 31

- OCT

- 02 21

- NOV

- 02

3538 3038

- NNMD 28

- OCT

- 02

3539 3039

- NNMD 28

- OCT

- 02

3540 3040 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3541 3041 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

3542 3042 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3543 3043 + P 28

- OCT

- 0 2 30

- OCT

- 02 21

- NOV

- 02

3544 3044 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

3545 3045 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

3546 3046 + P 28

- OCT

- 02 2

- NOV

- 02 24

- NOV

- 02

3547 3047 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

3548 3048 + TLL,P 28

- OCT

- 02 2

- NOV

- 02 24

- NOV

- 02

3549 3049 + P 28

- OCT

- 02 2

- NOV

- 02 24

- NOV

- 02

3550 3050

- NNMD 28

- OCT

- 02

3551 3051 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LIT TER LOSS P=PREGNANT23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 334

APPENDIX Q

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALA TION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Date of Date of Date of

Female No. Male No. Sperm/Plug Outcome Cohabitation Insemination Parturition

-----------------------------------------------------------------------------------------------------------------------------------

4526 4026 + P 28

- OCT

- 02 5

- NOV

- 02 27

- NOV

- 02

4527 4027 + P 28

- OCT

- 02 1

- NOV

- 02 23

- NOV

- 02

4528 4028 + P 28

- OCT

- 02 2

- NOV

- 02 24

- NOV

- 02

4529 4029 + P 28

- OCT

- 02 2

- NOV

- 02 24

- NOV

- 02

4530 4030 + NP 28

- OCT

- 02 2

- NOV

- 02

4531 4031 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

4532 4032 + P 28

- OCT

- 02 3

- NOV

- 02 25

- NOV

- 02

4533 4033 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

4534 4034 + P 28

- OCT

- 02 3

- NOV

- 02 24

- NOV

- 02

4535 4035 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

4536 4052 + P 28

- OCT

- 02 30

- OCT

- 02 21

- NOV

- 02

4537 4037 + P 28

- OCT

- 02 4

- NOV

- 02 26

- NOV

- 02

4538 4038 + P 28

- OCT

- 02 30

- OCT

- 02 20

- NOV

- 02

4539 4039 + NP 28

- OCT

- 02 30

- OCT

- 02

4540 4040 + P 28

- OCT

- 02 29

- OCT

- 02 20

- NOV

- 02

4541 4041

- N NMD 28

- OCT

- 02

4542 4042 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

4543 4043 + P 28 -O CT

- 02 30

- OCT

- 02 21

- NOV

- 02

4544 4044 + P 28

- OCT

- 0 2 29

- OCT

- 02 20

- NOV

- 02

4545 4045 + P 28 -O CT

- 02 5

- NOV

- 02 28

- NOV

- 02

4546 4046 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

4547 4047

- PNMD 28 -O CT

- 02 23

- NOV

- 02

4548 4048 + P 28

- OCT

- 02 31

- OCT

- 02 22

- NOV

- 02

4549 N DIED

4550 4050 + P 28

- OCT

- 02 8

- NOV

- 02 1

- DEC

- 02

4551 4051 + P 28

- OCT

- 02 30

- OC T- 02 21

- NOV

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE P=PREGNANT23

- JUN

- 2009 19:21

Huntingdon Life Sciences 00

- 4206 PAGE 335

APPENDIX R

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION O F

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS ( DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

1526 14 1 15 8 6 8 6 5 5 5 5 5 5 5 5 15 22

1527x DPP 14 0 14 3 9 0 0 0 0 0 0 0 0 0 0 15 22

1528 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 1 7 22

1529 PNMD 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 15 no day 0

1530 12 0 12 6 6 5 6 5 5 5 5 5 5 5 5 13 22

1531 16 0 16 7 9 7 9 5 5 5 5 5 5 5 5 17 22

1532x NP

1533 16 0 16 8 8 8 8 5 5 5 5 5 5 5 5 16 22

1534 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 14 22

1535x NP

1536 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 16 22

1537 13 0 13 10 3 10 3 7 3 7 3 7 3 7 3 15 22

1538 12 0 12 5 7 5 7 5 5 5 5 5 5 5 5 12 22

1539 13 0 13 7 6 7 6 5 5 5 5 5 5 5 5 13 22

1540 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22

1541x NP

1542 14 0 14 6 8 6 8 5 5 5 5 5 5 5 5 16 22

1543 16 0 16 9 7 9 7 5 5 5 5 5 5 5 5 16 22

1544 15 0 15 8 7 8 7 5 5 5 4 5 4 5 4 19 22

1545 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 15 22

1546 14 0 14 9 5 9 5 5 5 5 5 5 5 5 5 15 22

1547 14 0 14 11 3 11 3 7 3 7 3 7 3 7 3 14 22

1548xNNMD

1549 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 15 22

1550xNNMD

1551 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22

MEAN 14.3 0.1 14.3 7.5 6.8 7.4 6.8 5.2 4.8 5.2 4.8 5.2 4.8 5.2 4.8 15.1 22.0

S.D. 1.3 0.2 1.3 1.8 1.9 1.8 1.9 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 1.6 0.0

N 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 19

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

x =EXCLUDED FROM MEAN

DAY 4 COLUMN = PRECULLING23

- JUN

- 2009 19:21

Huntingdon Life Sciences 00

- 4206 PAGE 336

APPENDIX R

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

2526 12 1 13 5 6 5 6 5 5 4 5 4 5 4 5 14 22

2527xNNMD

2528 14 1 15 7 7 7 7 5 5 5 5 5 5 5 5 15 22

2529 12 0 12 6 6 6 6 5 5 5 5 5 5 5 5 14 22

2530 15 0 15 9 6 9 6 5 5 4 5 4 5 4 5 18 22

2531 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 16 22

2532x NP

2533 15 0 15 5 10 5 1 0 5 5 5 5 5 5 5 5 22

2534 6 0 6 2 4 2 4 2 4 2 4 2 4 2 4 14 23

2535 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 15 22

2536 15 1 16 4 11 4 10 4 6 4 6 4 6 4 6 17 22

2537 11 0 11 5 6 5 6 5 5 5 5 5 5 5 5 14 22

2538xNNMD

2539 14 0 14 7 6 7 6 5 5 5 5 5 5 5 5 16 22

2540 10 1 11 6 4 6 4 6 4 6 4 6 4 6 4 14 22

2541 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 16 22

2542xNNMD

2543 14 0 14 3 10 3 10 3 7 3 7 3 7 3 7 14 22

2544 4 0 4 2 2 2 2 2 2 2 2 2 2 2 2 13 23

2545 12 0 12 7 5 7 5 5 5 5 5 5 5 5 5 13 22

2546 12 1 13 6 6 6 6 5 5 5 5 5 5 5 5 14 22

2547 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 13 23

2548 11 0 11 6 5 6 5 5 5 5 5 5 5 5 5 15 22

2549 TLL 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0 7 23

2550 8 0 8 4 4 4 4 4 4 4 4 4 4 4 4 9 22

2551 15 0 15 4 10 4 10 4 6 4 6 4 6 4 6 15 22

MEAN 11.8 0.2 12.0 5.4 6.2 5.6 6.4 4.5 4.9 4.4 4.9 4.4 4.9 4.4 4.9 14.1 22.2

S.D. 3.8 0.4 3.8 2.4 2.5 2.1 2.2 1.0 0.9 1.0 0.9 1.0 0.9 1.0 0.9 2.4 0.4

N 22 22 22 22 22 21 21 21 21 21 21 21 21 21 21 21 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN

DAY 4 COLUMN = PRECULLING23

- JUN

- 2009 19:21

Huntingdon Life Sciences 00

- 4206 PAGE 337

APPENDIX R

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

3526x NP

3527 18 0 18 8 10 8 10 5 5 5 5 5 5 5 5 18 22

3528 12 0 12 3 8 3 5 3 5 3 5 3 5 3 5 14 21

3529 14 0 14 7 7 7 7 5 5 5 5 5 5 5 5 15 21

3530 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 15 22

3531 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22

3532 13 0 13 10 3 10 3 7 3 7 3 7 3 7 3 16 22

3533 13 1 14 7 6 7 6 5 5 5 5 5 5 5 5 15 22

3534 16 0 16 7 9 7 8 5 5 5 5 5 5 5 5 16 22

3535 15 0 15 6 9 6 9 5 5 5 5 5 5 5 5 16 22

3536 TLL 2 8 10 0 0 0 0 0 0 0 0 0 0 0 0 17 24

3537 17 0 17 9 8 9 8 5 5 5 5 5 5 5 5 17 21

3538xNNMD

3539xNNMD

3540 14 0 14 4 10 4 10 4 6 4 6 4 6 4 6 14 22

35 41 12 1 13 5 7 5 7 5 5 5 5 5 5 5 5 14 22

3542 14 0 14 10 4 10 4 6 4 6 4 6 4 6 4 15 22

3543 13 0 13 3 10 3 10 3 7 3 7 3 7 3 7 14 22

3544 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 14 22

3545 16 0 16 10 6 9 6 5 5 5 5 5 5 5 5 17 22

3546 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 17 22

3547 14 1 15 6 7 6 7 5 5 5 5 5 5 5 5 15 22

3548 TLL 12 1 13 0 0 0 0 0 0 0 0 0 0 0 0 13 22

3549 14 0 14 7 7 7 7 5 5 5 5 5 5 5 5 15 22

3550xNNMD

3551 6 0 6 6 0 6 0 6 0 6 0 6 0 6 0 14 22

MEAN 13.2 0.5 13.8 6.7 7.1 6.7 6.8 4.9 4.8 4.9 4.8 4.9 4.8 4.9 4.8 15.3 22.0

S.D. 3.4 1.7 2.4 2.1 2.5 2.0 2.5 0.9 1.3 0.9 1.3 0.9 1.3 0.9 1.3 1.3 0.6

N 22 22 22 20 20 20 20 20 20 20 20 20 20 20 20 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN

DAY 4 COLUMN = PRECULLING23

- JUN

- 2009 19:21

Huntingdon Life Scien ces 00

- 4206 PAGE 338

APPENDIX R

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

4526 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22

4527 14 0 14 8 6 8 5 5 5 5 5 5 5 5 5 15 22

4528 16 0 16 11 5 11 3 6 3 6 3 6 3 6 3 16 22

4529 12 0 12 9 3 9 3 7 3 7 3 7 3 7 3 15 22

4530x NP

4531 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 13 22

4532 17 0 17 5 5 5 5 5 5 5 5 5 5 5 5 17 22

4533 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22

4534 17 0 17 11 6 11 6 5 5 5 5 5 5 5 5 18 21

4535 9 0 9 4 5 4 5 4 5 4 5 4 5 4 5 15 22

4536 15 0 15 7 8 7 7 5 5 5 5 5 5 5 5 15 22

4537 11 0 11 5 6 5 6 5 5 5 5 5 5 5 5 14 22

4538 16 0 16 10 5 10 5 5 5 5 5 5 5 5 5 16 21

4539x NP

4540 12 0 12 6 6 6 6 5 5 5 5 5 5 5 5 14 22

4541xNNMD

4542 12 0 12 9 3 9 3 7 3 7 3 7 3 7 3 15 22

4543 15 0 15 6 9 6 9 5 5 5 5 5 5 5 5 15 22

4544 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22

4545 13 0 13 10 3 10 3 7 3 7 3 7 3 7 3 15 23

4546 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 17 22

4547 PNMD 14 0 14 4 10 4 10 4 6 4 6 4 6 4 6 15 no day 0

4548 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 15 22

4549xDIED

4550 16 0 16 8 8 7 8 5 5 5 5 5 5 5 5 17 23

4551 12 0 12 3 9 3 9 3 7 3 7 3 7 3 7 14 22

MEAN 14.1 0.0 14.1 7.2 6.5 7.1 6.4 5.1 4.8 5.1 4.8 5.1 4.8 5.1 4.8 15.3 22.0

S.D. 2.1 0.0 2.1 2.4 2.2 2.4 2.3 0.9 1.0 0.9 1.0 0.9 1.0 0.9 1.0 1.2 0.4

N 22 22 22 22 2 2 22 22 22 22 22 22 22 22 22 22 22 21

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE x=EXCLUDED FROM MEAN

DAY 4 COLUMN = PRECULLING23

- JUN

- 2009 19:24

Huntingdon Life Sciences 00

- 4206 PAGE 339

APPENDIX S

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

1526 FS MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4

1527 FD 0 FD 0 MU 1 MU 1 MU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1 FU 1

1528 MC 4 MK28 MK28 MK28 MC 4 MC 4 MK28 MK28 MC 4 FK28 FK28 FK28 FC 4 FK28 FK28

1529 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MC 4 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

1530 MK28 MK28 MK28 MD 2 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28

1531 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4 FC 4 FK28

1533 MK28 MC 4 MK28 MK28 MC 4 MC 4 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 F K28 FC 4 FC 4

1534 MK28 MK28 MK28 MK28 MC 4 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

1536 MC 4 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FC 4

1537 MC 4 MK28 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28

1538 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FC 4

1539 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

1540 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK 28

1542 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FC 4

1543 MC 4 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

1544 MC 4 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FD 9

1545 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 MC 4 FK28 FK28 FK28 FC 4 FK28 FK28

1546 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

1547 MK28 MK28 MK28 MC 4 MK28 MK28 MC 4 MK28 MK2 8 MC 4 MC 4 FK28 FK28 FK28

1549 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FC 4 FK28 FK28 FK28 FC 4 FK28

1551 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FC 4 FK28 FC 4 FC 4 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDI CATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCERTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE U

- UNSCHEDULED SACRIFICE23

- JUN

- 2009 19:24

Huntingdon Life Sciences 00

- 4206 PAGE 340

APPENDIX S

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

2526 MS FD 0 MM10 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FK28

2528 MS MK28 MK28 MK28 MC 4 MK28 MK28 MC 4 FC 4 FK28 FK28 FC 4 FK28 FK28 FK28

2529 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FC 4 FK28

2530 MC 4 MK28 MK28 MK28 MK28 MC 4 MM14 MC 4 MC 4 FK28 FK28 FK28 FC 4 FK28 FK28

2531 MK28 MC 4 MC 4 MC 4 MC 4 MK28 MK28 MC 4 MK28 MK28 FK28 FK2 8 FK28 FC 4 FK28 FK28

2533 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FC 4 FC 4 FC 4 FC 4 FK28 FK28 FK28 FK28

2534 MK28 MK28 FK28 FK28 FK28 FK28

2535 MC 4 MC 4 MK28 MK28 MC 4 MK28 MC 4 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28

25 36 MS MK28 MK28 MK28 MK28 FK28 FD 3 FC 4 FC 4 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

2537 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FK28

2539 FD 0 MK28 MC 4 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

2540 FZ MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

2541 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

2543 FD 0 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FC 4 FC 4 FK28 FK28

2544 MK28 MK28 F K28 FK28

2545 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

2546 FS MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28

2547 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4

2548 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

2549 FM 2 FM 1

2550 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

2551 MK28 MK28 MK28 MK28 FM 1 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4

SEX CODE PRECEDES PU P STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCERTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED M

- MISSING K

- SCHEDULED SACRIFICE23

- JUN

- 2009 19:24

Huntingdon Life Sciences 00

- 4206 PAGE 341

APP ENDIX S

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

3527 MK28 MC 4 MK28 MC 4 MK28 MK28 MK28 MC 4 FK28 FK28 FC 4 FC 4 FC 4 FC 4 FK28 FC 4 FK28 FK28

3528 MD 0 MK28 MK28 MK28 FD 4 FK28 FK28 FK28 FK28 FM 2 FM 2 FK28

3529 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 FC 4 FK28 FK28 FC 4 FK28 FK28 FK28

3530 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FK28

3531 MK28 MK28 MC 4 MC 4 MK28 MC 4 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FC 4 FK28

3532 MK28 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MK28 MC 4 FK28 FK28 FK28

3533 FS MK28 MK28 MC 4 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FK28

3534 MK28 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FD 4 FC 4 FK28 FK28 FC 4 FC 4

3535 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28 FC 4 FC 4

3536 U Z UZ MS MS MS FS FD 0 FS FS FD 0

3537 MK28 MC 4 MC 4 MK28 FK28 MC 4 MC 4 MK28 MK28 MK28 FC 4 FK28 FC 4 FK28 FC 4 FK28 FK28

3540 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FC 4 FK28

3541 MS MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

3542 M K28 MK28 MC 4 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 FK28 FK28 FK28 FK28

3543 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FC 4 FK28

3544 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28

3545 MK28 MK28 MK28 MD 2 MC 4 MC 4 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FK28 FC 4 FK28

3546 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FC 4 FK28 FK28 FC 4 FK28 FC 4 FK28

3547 MS MZ 1 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FC 4

3548 MS MZ 1 MZ 1 MM 1 MM 1 MM 1 MM 1 MM 1 FZ 1 FZ 1 FM 1 FZ 1 FM 1

3549 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

3551 MK28 MK28 MK28 MK28 MK28 MK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCERTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED M

- MISSING K

- SCHEDULED SACRIFICE23

- JUN

- 2009 19:24

Huntingdon Life Science s 00

- 4206 PAGE 342

APPENDIX S

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

4526 MC 4 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FK28

4527 MC 4 MC 4 MK28 MK28 MC 4 MK28 MK28 MK28 FD 3 FK28 FK28 FK28 FK28 FK28

4528 MK28 MK28 MK28 MK28 MC 4 MC 4 MM 5 MK28 MK28 MC 4 MC 4 FK28 FK28 FM 4 FK28 FM 4

4529 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

4531 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK 28 FK28 FK28 FC 4 FC 4 FK28

4532 MD 1 MK28 MK28 MK28 MK28 FK28 MK28 FD 1 FK28 FK28 FD 1 FK28 FD 1 FK28 FD 1 FD 1 FD 1

4533 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FC 4

4534 MK28 MK28 MK28 MC 4 MC 4 MK28 MC 4 MK28 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4

4535 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

4536 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FD 4 FC 4 FK28 FK28 FC 4

4537 MK28 MK28 MK28 MK28 MK2 8 FK28 FK28 FK28 FC 4 FK28 FK28

4538 FD 0 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

4540 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

4542 MK28 MK28 MK28 MK28 MK28 MK28 MC 4 M C 4 MK28 FK28 FK28 FK28

4543 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4 FC 4

4544 MK28 MC 4 MK28 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

4545 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 FK28 FK28 FK28

4546 MK28 MC 4 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 MC 4 FK28 FK28 FC 4 FK28 FK28 FK28

4547 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FK28

4548 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FC 4

4549 FOUND DEAD

4550 MD 4 MC 4 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FK28 FC 4 FK28 FK28 FC 4

4551 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCERTAIN

PUP STATUS CODES: A

- ALIVE D

- DIED C

- CU LLED M

- MISSING K

- SCHEDULED SACRIFICE23

- JUN

- 2009 19:29

Huntingdon Life Sciences 00

- 4206 PAGE 343

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1526 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

1527 PUP# 1 FOUND DEAD P

PUP# 2 FOUND DEAD P

PUP# 3 WITHIN NORMAL LIMITS P

PUP# 4 WITHIN NORMAL LIMITS P

PUP# 5 WITHIN NORMAL LIMITS P

PUP# 6 WITHIN NORMAL LIMITS P

PUP# 7 WITHIN NORMAL LIMITS P

PUP# 8 WITHIN NORMAL LIMITS P

PUP# 9 WITHIN NORMAL LIMITS P

PUP# 10 WITHIN NORMAL LIMITS P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P

PUP# 13 WITHIN NORMAL LIMITS P

PUP# 14 WITHIN NORMAL LIMITS P

1528 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 344

APPENDIX T

GASOLINE TBA VAPOR CONDE NSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1528 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

1529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMI TS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMA L LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

1530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PU P# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P

----------------------------------------------------------------------------------------------------------------------------- ----- -

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 345

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1530 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

1531 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN N ORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WI THIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

1533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 346

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL O BSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1533 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIM ITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORM AL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

PUP# 16 WITHI N NORMAL LIMITS P P

1534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

P UP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1536 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 347

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1536 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

1537 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1538 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23 -J UN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 348

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEM ALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1539 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 349

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1542 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

1543 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL L IMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NO RMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WIT HIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 350

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1544 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P

1545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMI TS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP # 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 351

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODU CTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LI MITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NOR MAL LIMITS P P P P P P

1547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

-------------------------------------------------------------------------------------------------------------- ---------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:26

Huntingdon Life Sciences 00

- 4206 PAGE 352

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHA LATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1549 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN N ORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WI THIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 353

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2526 PUP# 2 FOUND DEAD P

PUP# 3 WITHIN NORMAL LIMIT S P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

2528 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

2529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 354

APPEND IX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSER VATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2529 PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

P UP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

2530 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

2531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 355

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2531 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

2533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

2534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 356

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2535 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

2536 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN N ORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WI THIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 357

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

2539 PUP# 1 FOUND DEAD P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMI TS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMA L LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP # 14 WITHIN NORMAL LIMITS P P P P P P

2540 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

---------------------------------------------------------------------------------------------------------------------- -------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 358

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION R EPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2540 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

2541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL L IMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NO RMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WIT HIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 1 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

2543 PUP# 1 FOUND DEAD P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Scien ces 00

- 4206 PAGE 359

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINIC AL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2544 PUP# 1 WITHIN NORMAL LIMIT S P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

2545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP # 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

2546 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntin gdon Life Sciences 00

- 4206 PAGE 360

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVI DUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATION S LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2547 PUP# 1 WITH IN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

P UP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

2548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

2549 PUP# 1 WITHIN NORMAL LIMITS P

PUP# 2 WITHIN NORMAL LIMITS P

2550 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:30

Huntingdon Life Sciences 00

- 4206 PAGE 361

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE # OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2550 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

2551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 362

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3527 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PU P# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P

PUP# 17 WITHIN NORMAL LIMITS P P P P P P

PUP# 18 WITHIN NORMAL LIMITS P P P P P P

3528 PUP# 1 FOUND DEAD P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 363

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION W HOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

3530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMI TS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERA TE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 364

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3531 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

3532 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LI MITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NOR MAL LIMITS P P P P P P

3533 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

-------------------------------------------------------------------------------------------------------------------------------- ---

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 365

APPENDIX T

G ASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3533 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

3534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMIT S P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 W ITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

PUP# 16 WITHIN NORMAL LIMITS P P

35 35 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 366

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

I NHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3535 PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIM ITS P P

3536 PUP# 7 FOUND DEAD P

PUP# 10 FOUND DEAD P

3537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

PUP# 17 WITHIN NORMAL LIMITS P P P P P P

3540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 367

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3540 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

3541 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 200 9 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 368

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3542 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

3543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3544 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 369

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

3546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIM ITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHI N NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 370

APPENDIX T

GASOLINE TBA VA POR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3547 PUP# 2 WITHIN NORMAL LIMITS P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

3548 PUP# 2 WITHIN NORMAL LIMITS P

PUP# 3 WITHIN NORMAL LIMITS P

PUP# 4 WITHIN NORMAL LIMITS P

PUP# 5 WITHIN NORMAL LIMITS P

PUP# 6 WITHIN NORMAL L IMITS P

PUP# 7 WITHIN NORMAL LIMITS P

PUP# 8 WITHIN NO RMAL LIMITS P

PUP# 9 WITHIN NORMAL LIMITS P

PUP# 10 WIT HIN NORMAL LIMITS P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 1 2 WITHIN NORMAL LIMITS P

PUP# 13 WITHIN NORMAL LIMITS P

3549 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:31

Huntingdon Life Sciences 00

- 4206 PAGE 371

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPR ODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3549 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

3551 PUP# 1 WITHIN NORMAL LIMI TS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMA L LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 372

APPENDIX T

GASOLINE TBA VAPO R CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4526 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

4527 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIM ITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORM AL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PU P# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

----------------------------------------------------------------------------------------------------------------------------- ----- -

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 373

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION T OXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIM ITS P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORM AL LIMITS P

4529 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITH IN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

P UP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 374

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL L IMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

4532 PUP# 1 WI THIN NORMAL LIMITS P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P

PUP# 16 WITHIN NORMAL LIMITS P

PUP# 17 WITHIN NORMAL LIMITS P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 375

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMIT S P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

4534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

PUP# 17 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 376

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PU P# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

4536 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

4537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESEN T23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 377

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-------------------------------------------------------------------------------------------------------------------------- ---------

4537 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

4538 PUP# 1 FOUND DEAD P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

4540 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIM ITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORM AL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 378

APPENDIX T

GASOLINE TBA VAPOR CO NDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4540 PUP# 12 WITHIN NORMAL LIMITS P P

4542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

4543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN N ORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WI THIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 379

APPENDIX T

GASOLI NE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4544 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMIT S P P P P P P

4545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMA L LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP # 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 380

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIM ITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHI N NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

4547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

P UP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 381

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSER VATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL L IMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 1 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

4550 PUP# 1 WITHIN NORMAL LIMITS P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:33

Huntingdon Life Sciences 00

- 4206 PAGE 382

APPENDIX T

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

------------------------------------------------------------------------------------------------------------------ -----------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT23

- JUN

- 2009 19:41

Huntingdon Life Sciences 00

- 4206 PAGE 383

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 7.0 S 7.8 7.0 7.1 7.3 7.0 7.7 7.5 7.4 6.6 6.4 6.7 6.6 6.4 6.1

1527 5.7 D D 5.3 5.9 4.9 6.1 5.1 6.1 5.5 6.3 5.9 5.8 5.5 5.9

1528 7.0 7.0 8.3 6.2 6.7 6.5 7.3 6.8 7.8 7.5 7.1 6.8 6.2 6.9 7.0 6.6

1529 7.2 7.2 7.6 7.8 7.9 6.6 7.3 7.7 7.3 7.6 6.4 6.5 7.1 6.5 7.7 6.7

1530 7.3 7.7 7.2 7.9 7.6 6.4 7.8 7.5 6.9 6.4 7.8 6 .9 7.6

1531 6.3 6.8 6.0 6.7 6.3 6.9 6.7 5.5 5.9 6.2 6.5 6.6 5.9 5.8 6.1 6.3 6.3

1532 NOT PREGNANT

1533 6.2 6.2 6.3 6.4 6.6 6.2 7.1 6.7 5.8 5.4 5.8 6.6 5.5 5.3 6.0 6.1 6.4

1534 7.4 7.6 7.6 7.3 7.9 7.5 7.5 7.4 7.4 7.4 7.5 6.8 7.5 7.4

1535 NOT PREGNANT

1536 5.8 6.5 6.3 5.6 6.4 6.0 5.5 5.8 5.1 5.6 5.8 6.0 6.3 6.3 5.3 4.1

1537 7.4 7.6 8.1 7.1 6.9 7.7 7.2 6.9 7.8 7.9 7.2 7.0 7.7 7.5

1538 8.0 8.8 8.3 7.7 8.3 8.8 7.9 7.3 8.1 7.4 8.1 8.0 7.5

1539 6.9 7.0 7.8 6.6 6.8 7.1 6.4 7.2 6.6 6.9 6.2 6.9 7.5 6.5

1540 7.1 7.4 8.0 7.5 7.2 7.5 6.7 7.1 6.7 6.5 7.7 6.2 7.4 6.9 7.2 7.1

1541 NOT PREGNANT

1542 7.4 7.8 6.6 8 .2 7.3 8.4 8.0 7.0 7.7 4.6 7.9 7.5 7.8 7.6 7.8

1543 7.2 7.6 7.6 7.2 7.7 8.1 7.0 7.2 7.8 7.6 6.5 6.6 7.0 6.9 6.5 6.3 6.8

1544 7.0 7.4 7.5 7.7 7.7 7.6 7.2 7.1 7.0 6.4 6.9 6.9 5.8 7.0 6.9 5.4

1545 7.0 7.2 7.6 7.4 7.5 7.0 7.0 7.5 7.0 6.7 7.3 6.7 6.8 6.4 6.3 6.3

1546 7.0 6.8 7.5 6.3 7.1 7.9 7.7 7.5 7.5 6.7 6.8 6.9 5.7 6.4 6.7

1547 6.9 7.2 6.1 7.7 7.1 6.6 6.1 7.6 7.3 6.9 6.5 7.1 6.5 6.7 7.1

1548 NOT PREG., NO MATING DATE

1549 6.9 7.0 6.7 7.1 6.7 7.0 7.4 7.3 6.8 6.6 6.7 7.4 6.7 6.9

1550 NOT PREG., NO MATING DATE

1551 6.5 7.7 6.7 7.1 5.5 7.3 5.9 6.6 6.6 6.3 6.7 6.0 6.2 6.5 6.2 6.6

MEAN 7.0

S.D. 0.50

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED23

- JUN

- 2009 19:41

Huntingdon Life Sciences 00

- 4206 PAGE 384

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRA MS)

GROUP II 2000 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 7.5 S D 8.1 7.8 8.2 7.9 7.5 7.0 6.9 6.9 6.9 7.3 7.8

2527 NOT PREG., NO MATIN G DATE

2528 7.8 S 7.3 8.2 8.4 7.8 7.6 7.9 7.1 8.0 7.8 7.5 7.9 8.2 7.3 7.7

2529 7.9 8 .2 7.9 8.1 8.2 7.9 8.0 7.4 7.8 7.6 7.8 7.6 8.1

2530 7.3 8.3 7.3 7.1 7.1 6.9 7.4 7.2 7.5 7.4 7.5 7.7 7.2 6.5 6.9 8.2

2531 7.2 7.4 7.6 6.9 7.4 7.1 7.1 6.8 8.2 7.7 7.9 6.4 7.1 7.4 6.8 6.9 6.6

2532 NOT PREGNANT

2533 7.4 7.7 7.8 8.3 7.6 7.7 6.7 7.5 6.3 7.2 7.7 7.4 7.0 7.5 7.3 7.1

2534 8.8 9.6 9.3 8.1 8.8 8.3 8.6

2535 7.0 7.1 8.0 7.3 6.9 7.7 7.6 7.1 6.9 7.6 6.2 6.6 7.1 5.7 6.8 6.8

2536 6.3 S 7.0 6.8 6.9 5.7 6.2 6.4 6.3 6.6 6.8 6.2 6.0 6.0 6.1 6.8 5.2

2537 7.3 7.3 7.1 8.0 7.9 7.5 7.0 7.4 6.9 7.5 7.1 6.8

2538 NOT PREG., NO MATING DATE

2539 6.8 D 6.2 7.6 6.9 7.3 6.7 7.7 7.6 6.0 6.6 6.9 6.8 6.3 6.4

2540 7.4 Z 7.5 7.0 7.7 7.9 7.7 7.3 6.9 7.5 7.2 7.2

2541 7.5 7.2 7.6 7.3 8.1 8.1 7.9 6.9 7.8 7.4 7.1 7.4 7.3 7.2

2542 NOT PREG., NO MATING DATE

2543 7.5 D 7.6 7.3 7.3 7.8 7.7 7.0 7.2 7.6 7.0 7.5 7.4 7.7 8.4

2544 9.5 9.8 9.6 8.9 9.5

2545 7.0 7.3 7.3 7.6 7.3 7.3 6.6 7.3 6.5 7.0 7.0 6.4 6.7

2546 7.4 S 7.8 7.4 7.6 7.6 7.8 8.2 7.6 7.1 7.4 7.2 7.3 6.3

2547 7.4 7.1 6.9 7.8 7.2 7.0 7.5 7.9 7.5 7.7 7.0 7.3 7.5 8.0

2548 7.9 8.3 8.3 7.3 7.6 8.6 8.2 8.1 7.6 7.6 7.2 8.0

2549 6.2 6.2 M

2550 8.6 8.9 8.5 8.6 9.0 8.4 8.4 8.3 8.4

2551 7.5 8.3 8.2 7.9 8.2 M 7.2 6.8 7.5 7.3 7.4 7.2 7.7 7.4 6.8 7.0

MEAN 7.5

S.D. 0.73

N 22

PUP ST ATUS CODES: S

- STILLBORN D

- DIED M

- MISSING Z- CANNIBALIZED23

- JUN

- 2009 19:41

Huntingdon Life Sciences 00

- 4206 PAGE 385

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FE MALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREGNANT

3527 6.8 7.6 7.7 6.2 6.9 6.8 7.5 6.3 7.5 6.9 6.8 6.1 7.1 7.1 7.2 6.0 6.7 6.1 6.4

3528 5.6 D 6.4 6.1 5.6 5.4 5.7 5.6 5.6 5.3 4.9 4.9 5.8

3529 6.4 6.1 6.7 7.0 6.6 5.9 6.4 6.4 6.1 6.1 6.7 6.7 6.2 6.5 6.3

3530 6.6 6.9 6.3 7.2 7.0 6.7 6.5 6.3 6.1 6.5 6.3 6.6 6.6 6.5

3531 7.1 7.2 7.6 7.2 7.1 7.6 7.6 7.4 7.1 6.6 7.3 6.9 7.3 6.5 6.4 7.0

3532 7.0 7.6 7.4 7.3 6.9 6.6 6.9 7.1 6.3 7.2 6.9 6.6 7.0 7.0

3533 7.3 S 7.1 7.7 6.1 7.4 7.7 8.1 7.6 7.5 7.1 7.2 6.5 7.6 7.4

3534 6.4 6.5 6.8 6.8 6.6 6.3 7.0 6.1 6.1 6.6 6.5 6.7 6.2 6.7 5.8 6.6 5.8

3535 6.7 6.0 7.3 6.8 7.5 7.0 7.2 6.9 6.5 6.6 5.7 6.1 5.7 7.0 6.4 7.2

3536 TOTAL LITTER LOSS

3537 5.8 5.9 5.7 6.0 5.1 6.5 5.8 5.9 6.1 6.3 5.8 5.7 5.3 5.3 5.8 5.9 5.1 6.0

3538 NOT PREG., NO MATING DATE

3539 NOT PREG., NO MATING DATE

3540 6.7 6.7 7.5 7.7 6.9 5.8 6.2 6.3 7.1 6.5 6.2 7.0 7.0 6.9 6.7

3541 6.7 S 7.5 6.8 6.7 6.4 7.2 5.8 7.4 5.9 6.9 6.5 6.4 6.8

3542 7.7 7.8 7.8 7.6 7.9 7.6 7.6 7.8 8.1 7.8 8.3 6.9 7.4 7.7 6.9

3543 7.1 7.7 7.5 7.5 6.8 7.2 7.0 6.8 7.2 7.0 6.1 6.9 7.2 7.4

3544 7.0 7.6 7.8 7.0 7.4 7.1 7.2 3.9 7.2 6.8 7.6 7.2 7.2 7.6

3545 6.5 5 .9 6.8 6.3 6.3 6.3 6.3 7.2 7.1 7.3 6.3 6.6 5.7 6.3 6.7 6.8 6.6

3546 7.5 7.8 7.6 7.5 7.6 8.1 7.4 7.8 7.0 7.9 7.3 7.7 7.4 7.3 7.6 7.2

3547 7.1 S Z 7.6 7.1 7.4 6.9 7.4 6.7 7.4 7.0 7.1 7.0 7.1 6.9 7.1

3548 TOTAL LITTER LOSS

3549 6.9 7.6 7.6 6.9 7.2 6.6 7.1 6.5 7.3 6.5 6.8 6.6 6.9 6.8 6.4

3550 NOT PREG., NO MATING DATE

3551 9.0 8.7 9.3 9.6 9.0 8.9 8.5

MEAN 6.9

S.D. 0.71

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED Z

- CANNIBALIZED23

- JUN

- 2009 19:41

Huntingdon Life Sciences 00

- 4206 PAGE 386

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATI ON REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 6.6 6.8 7.0 6 .6 6.7 6.4 7.0 6.6 6.6 6.9 6.3 7.0 5.9 6.7 6.1 5.9

4527 7.4 7.9 7.5 7.7 7.6 7.5 7.7 7.9 7.7 7.2 7.0 6.9 7.0 7.2 7.2

4528 6.9 7.8 7.0 6.5 7.7 6.9 7.3 6.8 6.5 6.9 7.1 6.7 7.2 6.6 6.8 6.8 6.1

4529 7.6 8.1 7.0 7.8 7.5 8.2 7.9 7.6 7.9 7.5 6.8 6.8 8.1

4530 NOT PRE GNANT

4531 6.6 6.5 7.1 5.9 6.8 6.9 7.0 6.7 6.8 6.9 6.4 5.9 6.1 6.5

4532 5.6 D 6.0 6.4 6.0 6.5 4.6 6.4 D 5.3 5.9 D 4.6 D 4.7 D D D

4533 7.3 7.7 7.5 7.2 7.2 7.8 7.3 7.2 7.1 7.1 7.8 7.4 7.1 6.3 7.4 7.1

4534 6.2 6.3 6.0 6.7 6.3 6.3 6.7 6.4 5.7 6.5 6.6 6.3 6.0 5.7 6.2 5.2 6.7 6.3

4535 7.9 8.4 8.5 8.3 8.8 7.1 6.1 8.4 7.8 7.6

4536 5.9 5.5 6.5 6.1 6.1 5.9 5.2 6.3 5.4 5.7 5.9 5.9 5.9 6.3 5.5 5.6

4537 6.7 6.2 7.3 6.7 6.3 6.7 6.5 6.6 7.5 6.7 6.4 6.9

4538 6.7 D 7.3 6.8 7.3 6.7 7.1 7.1 6.5 6.8 6.8 7.0 6.6 5.6 5.7 6.8 6.0

4539 NOT PREGNANT

4540 7.0 7.8 7.2 7.2 6.6 7.5 6.9 6.8 6.6 6.6 7.5 7.1 6.2

4541 NOT PREG., NO MATING DATE

4542 6.6 6.8 5.8 7.0 6.9 6.8 6.7 6.5 6.9 6.8 6.4 6.6 6.3

4543 6.7 7.2 6.8 6.0 7.0 6.7 6.3 7.2 7.0 6.9 6.0 6.7 7.0 6 .4 6.9 6.7

4544 6.7 7.3 7.4 6.2 7.8 6.5 5.8 6.4 7.4 7.0 6.1 6.9 6.2 6.5 6.8 5.9

4545 8.3 8.0 8.7 8.4 8.2 8.5 8.8 8.5 8.0 8.4 8.7 8.0 8.0 8.0

4546 7.5 8.0 7.6 7.7 7.8 7.4 7.5 7.8 7.8 8.2 7.7 6.8 7.3 7.8 6.5 7.5 6.8

4547 6.9 7.6 6.9 7.1 6.9 6.4 6.7 6.9 6.6 6.6 7.0 6.9 7.4 7.0 6.5

4548 7.0 7.9 6.5 7.1 7.0 7.0 6.3 6.6 6.7 7.1 7.2 7.2 7.7 6.2 7.3 6.9

4549 FOUND DEAD

4550 7.5 6.7 7.6 7.7 7.8 8.6 7.9 8.5 7.6 7.3 7.8 7.1 7.5 6.9 7.2 6.8 6.6

4551 7.7 8.3 8.1 7.8 7.5 7.8 7.1 7.7 6.8 7.8 7.8 7.9 7.4

MEAN 7.0

S.D. 0.64

N 22

PUP STATUS CODES: D

- DIED23

- JUN

- 2009 19:44

Huntingdon Life Sciences 00

- 4206 PAGE 387

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 9.9 S 10.9 9.6 10.1 10.0 9.8 10.8 10.4 10.7 9.8 9.0 10.0 9.5 9.6 9.1

1527 DIED POST PARTUM

1528 9.3 9.6 9.9 8.6 9.7 8.9 9.3 9.1 10.8 9.4 9.8 9.0 8.7 8.9 8.9 9.4

1529 10.3 10.2 10.6 10.9 10.6 9.3 10.4 10.4 10.4 11.0 9.5 9.9 10.2 9.6 11.0 10.0

1530 10.9 10.9 10.8 11.6 D 9.5 11.9 11.5 10.8 9.4 11.5 10 .3 11.2

1531 9.7 10.8 9.8 10.5 9.3 11.2 10.6 8.5 9.1 10.0 10.1 9.7 9.2 8.4 9.1 10.1 8.4

1532 NOT PREGNANT

1533 8.9 8.8 9.6 8.7 9.3 8.5 10.0 10.0 8.9 8.1 8.1 9.4 8.6 8.4 8.8 8.8 9.2

1534 11.2 11.6 11.6 11.2 12.0 11.3 11.5 11.3 11.1 11.2 11.0 9.9 11.4 11.1

1535 NOT PREGNANT

1536 6.1 6.9 6.7 6.0 7.4 6.3 5.8 6.4 5.6 5.9 5.9 6.1 6.6 7.0 5.1 4.1

1537 9.9 10.3 9.9 10.5 9.7 10.7 9.8 9.4 9.9 10.4 9.7 9.5 9.3 9.7

1538 11.9 12.5 12.5 11.7 12.0 12.4 11.0 14.6 12.0 10.7 11.4 11.2 10.9

1539 9.8 9.3 11.2 9.7 9.2 9.7 8.9 10.6 9.6 10.7 8.6 10.0 10.8 8.8

1540 10.8 11.2 11.4 11.7 11.4 10.8 9.9 11.1 9.9 10.0 11.9 8.5 11.2 11.5 10.8 11.3

1541 NOT PREGNANT

1542 10.7 10.9 8.4 12 .2 10.2 12.6 11.1 9.9 10.8 6.3 12.0 11.0 11.0 11.6 11.4

1543 10.2 11.1 10.7 10.0 11.3 10.7 10.3 10.2 10.8 10.9 10.0 9.4 10.6 9.3 9.0 9.0 10.2

1544 10.4 11.0 11.5 11.7 11.5 11.5 10.4 9.9 10.2 9.8 10.6 9.8 8.7 10.8 10.7 8.0

1545 10.0 10.4 11.0 10.6 11.2 9.6 10.4 10.1 10.0 9.9 10.4 9.2 10.0 8.9 8.8 8.8

1546 9.3 8.9 9.6 7.9 10.7 10.6 10.2 10.0 9.2 9.1 9.4 9.3 7.6 8.6 9.0

1547 9.3 9.0 9.1 9.8 10.1 9.3 8.8 10.1 9.7 9.2 8.1 9.5 9.0 9.1 9.2

1548 NOT PREG., NO MATING DATE

1549 10.2 10.1 10.0 10.4 9.7 10.3 10.8 10.7 10.1 10.2 10.1 10.6 9.6 10.2

1550 NOT PREG., NO MATING DATE

1551 9.6 11.8 10.0 10.1 7.6 10.7 8.9 9.8 9.7 9.4 9.4 8.9 9.1 9.9 9.0 9.8

MEAN 9.9

S.D. 1.15

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED23

- JUN

- 2009 19:44

Huntingdon Life Sciences 00

- 4206 PAGE 388

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRA MS)

GROUP II 2000 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 11.2 S D 12.1 11.7 12.2 11.7 11.2 10.1 10.3 10.7 10.2 11.1 11.8

2527 NOT PREG., NO MATIN G DATE

2528 10.9 S 10.8 11.6 11.7 11.4 10.4 10.7 10.1 10.9 11.1 11.2 11.3 11.2 9.8 10.8

2529 11.6 11 .5 11.5 12.1 11.7 11.7 11.8 11.1 11.6 11.8 11.2 11.5 12.0

2530 10.5 11.7 11.0 10.8 10.6 9.7 11.2 7.1 11.1 11.3 10.7 11.3 10.8 10.4 10.0 10.5

2531 9.2 9.9 9.8 8.3 10.0 8.9 9.3 9.3 9.5 9.3 10.5 7.9 9.4 9.4 8.4 8.2 8.7

2532 NOT PREGNANT

2533 9.7 10.6 9.7 10.9 10.4 10.1 8.6 9.4 8.8 9.9 9.9 9.7 8.9 9.6 10.0 9.2

2534 12.6 13.2 13.5 11.6 13.1 11.5 12.8

2535 8.7 9.6 9.6 9.0 8.3 9.5 9.2 8.5 9.1 8.2 7.2 7.9 9.1 7.6 8.5 9.2

2536 8.9 S 9.6 10.0 9.9 8.1 8.8 D 8.9 9.1 9.9 8.1 7.8 8.1 8.4 9.9 7.6

2537 11.0 11.0 9.7 11.5 11.8 10.6 10.8 11.3 10.7 11.5 11.4 10.5

2538 NOT PREG., NO MATING DATE

2539 10.1 D 9.6 11.1 9.7 10.7 9.5 11.5 10.8 9.1 9.9 10.2 10.5 9.2 9.8

2540 11.2 Z 11.2 11.0 11.7 11.8 11.6 10.8 10.5 10.9 11.4 11.1

2541 11.7 11.2 12.1 12.0 12.0 12.6 12.0 11.1 12.0 12.1 10.9 11.7 11.2 11.3

2542 NOT PREG., NO MATING DATE

2543 11.0 D 11.4 10.9 10.8 11.3 11.5 10.6 10.7 10.7 10.3 11.1 10.8 11.5 11.6

2544 13.8 14.0 14.2 13.3 13.8

2545 10.5 11.1 10.7 11.3 10.7 10.7 9.4 10.7 9.9 10.8 10.6 9.1 10.4

2546 11.1 S 11.5 11.2 11.3 11.2 12.2 12.2 10.9 10.6 10.9 10.6 11.0 9.0

2547 10.3 10.4 10.4 10.7 10.2 9.4 10.5 10.6 10.3 10.8 9.6 10.0 10.1 10.6

2548 11.9 12.3 12.2 11.3 11.7 12.6 11.9 12.2 11.8 11.7 11.7 11.7

2549 TOTAL LITTER LOSS

2550 14.1 14.1 14.0 13.9 14.7 14.5 13.4 14.0 13.9

2551 10.9 12.1 11.1 11.7 11.4 M 10.6 10.3 11.0 10.1 10.3 11.0 11.0 11.7 9.9 10.5

MEAN 11.0

S.D. 1.39

N 21

PUP ST ATUS CODES: S

- STILLBORN D

- DIED M

- MISSING Z

- CANNIBALIZED23

- JUN

- 2009 19:44

Huntingdon Life Sciences 00

- 4206 PAGE 389

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FE MALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREGNANT

3527 10.2 11.9 11.2 9.9 10.7 11.2 9.8 10.3 10.9 10.2 9.6 8.5 10.7 10 .2 9.6 9.3 9.4 9.4 10.3

3528 8.0 D 8.5 8.4 8.2 D 7.7 8.0 7.8 7.5 M M 7.9

3529 8.7 7.8 9.1 9.7 9.4 7.8 9.0 8.3 8.9 8.5 9.6 8.4 8.8 8.9 8.2

3530 9.8 10.0 9.3 10.6 10.0 10.0 10.0 9.8 9.6 9.4 9.4 10.0 9.3 9.5

3531 9.8 9.7 9.7 9.4 10.4 10.1 10.9 10.4 10.3 9.3 9.7 9.4 9.3 9.5 8.6 9.6

3532 10.6 11.1 11.4 11.3 10.5 10.6 10.2 10.6 9.1 10.9 10.9 10.3 10.6 10.8

3533 10.3 S 9.9 10.7 8.8 10.6 10.6 11.0 10.3 10.8 9.6 10.6 9.8 10.4 10.4

3534 9.5 9.1 9.4 10.0 9.7 9.4 9.5 9.2 9.1 10.4 9.8 D 9.3 10.0 8.3 9.8 9.0

3535 9.2 8.5 9.9 9.5 10.3 9.6 9.7 9.6 9.2 9.3 7.3 8.5 8.0 9.5 9.2 9.5

3536 TOTAL LITTER LOSS

3537 7.9 7.9 7.4 8.5 7.6 8.7 7.3 7.9 7.8 8.5 8.0 8.0 7.5 7.9 8.1 7.7 6.6 8.6

3538 NOT PREG., NO MATING DATE

3539 NOT PREG., NO MATING DATE

3540 9.8 9.6 10.8 10.8 10.1 8.4 8.9 9.8 10.2 9.3 9.3 9.9 10.2 9.7 9.5

3541 10.0 S 10.9 9.9 9.9 10.1 10.4 9.3 10.7 9.1 10.5 9.8 9.3 10.1

3542 11.0 10.8 11.0 10.7 12.0 10.6 11.1 11.5 11.5 11.8 11.7 10.0 9.9 11.3 10.6

3543 9.9 10.7 9.9 10.7 9.8 9.9 9.9 9.6 10.0 10.2 8.5 9.5 9.8 10.5

3544 10.2 11.4 11.5 10.6 10.8 10.4 10.2 5.0 10.6 9.9 10.7 10.4 9.8 10.8

3545 9.4 8 .6 9.8 9.8 D 8.9 9.4 10.2 9.9 10.0 10.0 8.8 8.2 9.0 9.6 9.9 9.6

3546 10.9 11.4 11.2 10.7 11.3 12.0 10.3 11.2 9.9 11.4 10.2 11.0 10.4 10.6 10.9 10.3

3547 9.5 S Z 9.8 9.3 10.1 9.1 10.4 9.0 9.9 9.2 9.1 9.2 9.4 9.2 9.8

3548 TOTAL LITTER LOSS

3549 9.9 10.6 10.3 9.8 10.4 9.6 10.2 9.4 10.0 9.4 10.2 9.4 9.5 9.7 9.5

3550 NOT PREG., NO MATING DATE

3551 15.8 15.2 16.4 16.6 15.8 15.5 15.2

MEAN 10.0

S.D. 1.58

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED Z

- CANNIBALIZED M

- MISSING23

- JUN

- 2009 19:44

Huntingdon Life Sciences 00

- 4206 PAGE 390

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATI ON REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 8.4 8.2 8.8 8 .3 8.0 8.4 9.2 8.2 8.9 9.1 8.4 8.9 8.1 8.5 8.0 7.7

4527 10.7 11.5 10.4 10.7 11.4 10.7 10.4 11.4 10.9 D 10.2 11.2 9.6 10.2 10.2

4528 8.5 7.5 8.9 7.6 10.1 10.3 9.4 7.7 6.6 7.3 9.0 9.4 7.5 8.7 M 8.4 M

4529 10.2 11.2 9.2 11.1 10.9 11.2 9.7 9.3 10.9 9.6 9.3 9.4 10.4

4530 NOT PRE GNANT

4531 9.2 8.9 9.6 8.7 9.2 9.7 9.7 8.8 9.4 9.6 9.2 8.9 8.3 9.5

4532 7.9 D 8.5 9.0 8.2 8.7 6.4 9.1 D 7.7 8.4 D 6.5 D 6.1 D D D

4533 10.4 11.5 10.7 9.6 9.6 10.8 10.8 10.2 10.4 10.0 10.4 10.7 9.6 9.4 10.6 11.0

4534 9.1 8.9 9.4 10.1 9.5 9.5 9.9 9.0 8.0 10.0 9.0 9.3 9.6 8.6 8.3 7.4 9.2 9.2

4535 11.7 12.3 13.0 12.0 12.7 11.0 9.3 12.0 11.9 11.5

4536 8.1 8.0 8.3 9.2 8.4 7.9 7.3 7.5 8.1 8.4 8.3 D 7.9 8.6 8.0 7.7

4537 10.8 9.8 12.2 10.6 9.9 10.5 10.3 10.7 12.3 10.7 10.3 11.1

4538 10.1 D 11.5 9.7 11.1 10.3 10.4 10.8 9.7 10.8 10.4 10.4 10.0 8.8 8.5 10.5 9.1

4539 NOT PREGNANT

4540 10.5 11.6 11.0 11.0 9.6 11.3 10.4 10.4 10.3 9.9 10.8 10.8 9.1

4541 NOT PREG., NO MATING DATE

4542 9.1 9.0 7.9 10.3 10.4 9.2 8.8 8.6 9.4 9.0 9.0 9.0 8.4

4543 10.2 10.7 10.6 9.3 10.8 10.4 10.1 10.6 10.2 10.0 9.6 10.3 10.5 10 .2 10.6 9.6

4544 9.6 10.5 9.6 9.7 10.7 9.4 8.3 9.7 10.7 10.3 8.7 10.5 9.1 9.5 9.3 8.4

4545 10.9 10.7 11.1 11.4 10.6 11.4 11.8 11.2 10.7 10.4 11.2 9.9 10.3 10.6

4546 10.1 10.3 10.2 9.6 10.6 9.5 10.4 10.4 10.0 11.3 10.5 9.5 10.1 10.6 9.5 9.8 9.7

4547 9.8 9.6 9.9 9.9 9.7 9.5 9.7 10.0 10.5 9.5 9.7 9.8 10.0 10.1 8.9

4548 10.3 11.6 9.0 10.0 10.4 10.0 9.8 10.2 9.7 10.4 10.6 11.1 10.9 9.3 11.6 9.9

4549 FOUND DEAD

4550 10.7 D 10.8 10.7 11.0 11.9 11.4 11.9 11.1 10.8 11.5 10.7 9.7 9.8 10.0 9.9 9.4

4551 10.8 11.7 11.7 10.9 10.5 10.6 10.7 10.9 9.7 11.2 10.9 10.7 10.4

MEAN 9.9

S.D. 1.02

N 22

PUP STATUS CODES: D

- DIED M

- MISSING23

- JUN

- 2009 19:45

Huntingdon Life Sciences 00

- 4206 PAGE 391

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 13.8 S 14.4 13.6 13.8 14.4 13.6 C C C 13.9 13.0 14.0 13.4 13.4 C

1527 DIED POST PARTUM

1528 12.8 C 13.3 11.5 13.2 C C 12.0 14.5 C 13.2 12.3 11.9 C 11.9 13.7

1529 14.3 14.2 14.4 14.9 C C C 14.2 C 15.2 13.7 14.0 14.2 12.8 15.0 C

1530 14.2 14.6 14.2 15.3 D 12.3 15.6 14.7 C 12.5 14.6 13 .5 14.7

1531 14.1 C 14.3 C 14.4 16.5 15.2 12.6 12.8 14.6 C C 13.4 12.8 C C 14.2

1532 NOT PREGNANT

1533 13.0 13.1 C 13.2 13.6 C C 14.3 13.3 11.9 12.4 C 12.6 12.8 12.9 C C

1534 15.8 15.9 16.3 15.8 16.7 C 15.9 C C 15.2 15.4 14.6 15.5 16.2

1535 NOT PREGNANT

1536 7.7 C 8.8 7.3 8.9 7.4 6.9 C 6.8 7.9 7.3 7.1 8.5 C C C

1537 13.6 C 13.6 14.3 13.2 14.7 14.0 12.7 C C 13.6 13.4 13.4 13.6

1538 16.5 17.6 17.2 16.7 16.5 17.4 15.9 14.7 17.1 C 16.1 15.8 C

1539 13.9 C C 14.2 13.8 13.6 12.6 15.1 14.3 15.3 12.2 C 14.9 12.7

1540 16.4 16.7 17.7 16.9 16.5 15.8 C 16.5 C 14.7 C C C 16.2 15.5 17.1

1541 NOT PREGNANT

1542 14.0 C 11.7 16 .3 14.2 15.9 15.2 13.6 14.1 8.6 16.1 C C 14.6 C

1543 14.9 C 15.6 15.0 16.2 C C C 16.2 16.3 14.7 13.9 C C 12.9 13.0 15.0

1544 15.6 C 16.5 17.3 17.2 18.2 C C 16.0 15.0 16.3 14.8 13.0 C C 12.1

1545 14.2 15.2 15.2 16.2 C C C 14.6 14.1 C 15.0 12.8 13.5 C 12.4 12.6

1546 12.5 12.4 12.9 11.5 13.9 C C 13.6 C C 12.6 13.2 10.9 11.5 12.5

1547 12.8 12.4 12.1 13.9 C 12.6 12.4 C 13.3 12.4 C C 13.3 12.7 12.8

1548 NOT PREG., NO MATING DATE

1549 13.6 11.4 13.9 13.7 13.2 13.8 C 14.6 C 13.9 13.7 14.4 C 13.8

1550 NOT PREG., NO MATING DATE

1551 14.4 17.2 15.0 15.3 11.7 14.5 13.5 C 14.9 14.0 C C 13.7 C C 14.5

MEAN 13.9

S.D. 1.85

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED23

- JUN

- 2009 19:45

Huntingdon Life Sciences 00

- 4206 PAGE 392

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAM S)

GROUP II 2000 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 14.0 S D 11.5 14.6 15.2 14.6 14.7 C 13.5 13.7 13.2 14.3 14.7

2527 NOT PREG., NO MATING DATE

2528 14.8 S 14.2 16.2 15.3 C 13.6 15.3 C C 14.3 14.7 C 15.2 13.7 15.4

2529 14.6 14. 7 14.9 15.0 14.6 14.8 C 14.3 14.4 14.5 14.1 C 15.1

2530 15.5 C 17.7 16.1 16.5 15.2 C 7.9 C C 16.9 17.1 16.2 C 15.3 16.4

2531 12.7 14.0 C C C C 12.7 12.9 C 13.7 13.9 11.2 12.8 12.4 C 10.8 12.3

2532 NOT PREGNANT

2 533 13.7 14.4 13.6 15.0 14.3 14.3 12.0 C C C C C 12.8 13.6 14.0 13.1

2534 15.6 16.3 16.6 14.7 15.8 13.7 16.3

2535 11.9 C C 12.6 11.9 C 13.0 C 11.9 11.9 10.2 C 12.5 10.3 11.8 12.5

2536 13.7 S 15.4 14.9 14.9 12.7 13.2 D C C 14.6 12.0 12.4 C C 15.0 12.0

2537 14.0 14.0 13.1 14.6 14.9 13.8 C 14.1 13.6 14.6 14.3 13.2

2538 NOT PREG., NO MATING DATE

2539 13.8 D 13.3 C 13.5 15.1 C 15.7 14.5 12.7 13.9 14.1 C 12.2 12.6

2540 14.7 Z 15.0 14.4 15.3 14.8 15.6 13.9 13.8 14.6 14.9 14.4

2541 16.8 16.1 16.6 17.3 18.2 17.2 C 15.7 17.0 17.3 C C 15.9 16.6

2542 NOT PREG., NO MATING DATE

2543 15.6 D 15.7 15.4 15.0 16.0 15.4 15.2 C 15.8 14.5 C C 16.8 15.8

2544 16.9 18.1 15.8 16.5 17.0

2545 13.9 14.9 13.6 15.2 14.2 14.1 C C 12.8 14.6 13.9 12.3 13.9

2546 14.8 S 15.6 15.2 C 15.3 16.1 16.8 14.5 13.7 C 14.1 14.1 12.3

2547 13.4 13.1 13.6 13.9 C 12.7 13.8 13.6 13.5 13.8 12.8 13.0 C C

2548 15.1 14.8 C 14.3 15.3 15.3 14.8 16.0 15.8 14.5 15.0 15.5

2549 TOTAL LITTER LOSS

2550 18.0 18.7 17.5 17.7 18.5 18.3 17.3 18.0 17.6

2551 15.7 16.9 15.8 16.9 16.3 M C C 15.5 14.4 15.3 16.0 15.6 C 14.5 C

MEAN 14.7

S.D. 1.44

N 21

PUP STA TUS CODES: S

- STILLBORN D

- DIED M

- MISSING Z

- CANNIBALIZED23

- JUN

- 2009 19:45

Huntingdon Life Sciences 00

- 4206 PAGE 393

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WH OLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMA LE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREGNANT

3527 15.2 17.1 C 15.0 C 16.6 14.9 16.0 C 15.1 14.7 C C C C 13.5 C 14.1 15.3

3528 10.4 D 10.9 10.8 10.3 D 10.0 10.1 10.5 9.9 M M 10.3

3529 13.0 11.6 13.7 13.4 14.0 C C 12.1 C 12.3 14.2 C 13 .3 12.9 12.4

3530 14.0 13.8 14.3 C 14.2 14.4 13.8 14.1 C 13.8 13.6 14.0 C 14.1

3531 14.0 13.9 14.1 C C 14.1 C 14.3 14.5 C 14.2 14.0 13.5 13.5 C 13.5

3532 14.6 15.4 15.1 15.6 14.1 14.6 14.3 C C 14.3 C 13.7 14.3 14.7

3533 14.1 S 13.8 14.6 C 14.4 C 14.9 14.6 14.3 13.2 14.1 13.3 C 14.0

3534 13.9 14.0 C C 14.2 13.5 14.2 13.8 13.4 14.3 14.4 D C 14.3 12.4 C C

3535 12.3 11.4 13.4 C 13.8 12.6 12.4 13.0 11 .9 C C 11.0 10.7 12.4 C C

3536 TOTAL LITTER LOSS

3537 11.6 11.6 C C 11.4 12.5 C C 11.2 12.2 12.0 C 11.1 C 11.6 C 9.6 12.6

3538 NOT PREG., NO MATING DATE

3539 NOT PREG., NO MATING DATE

3540 13.3 13.2 14.5 14.7 14.5 11.5 12.3 C C C 13.2 13.0 13.4 C 12.6

3541 15.0 S 16.1 14.5 14.9 15.3 15.1 13.7 15.9 C C 14.7 13.9 15.5

3542 15.5 15.3 15.4 C 16.8 15.1 C C C 16.7 16.5 14.3 14.1 15.5 14.9

3543 13.3 14.1 13.2 14.3 12.9 13.5 13.2 12.8 13.5 C 11.6 C C 13.6

3544 13.3 15.4 15.0 13.7 14.3 13.7 C 5.9 14.0 13.2 14.3 C C 13.6

3545 12.6 11.7 13.4 13.1 D C C 14.3 C 13.3 C 11.7 11.3 11.8 12.9 C 12.7

3546 14.9 15.3 15.5 15.1 15.0 15.8 C C 13.8 C 14.2 15.3 C 15.0 C 14.2

3547 12. 6 S Z 12.3 12.1 13.2 C 13.2 11.8 13.6 12.6 12.3 C 13.1 11.4 C

3548 TOTAL LITTER LOSS

35 49 13.5 C C 14.1 14.0 13.1 14.0 13.0 C C 13.5 13.8 12.7 13.5 12.9

3550 NOT PREG., NO MATING DATE

3551 21.2 20.7 21.7 22.4 21.2 21.0 20.4

MEAN 13.9

S.D. 2.14

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED M

- MISSING23

- JUN

- 2009 19:45

Huntingdon Life Sciences 00

- 4206 PAGE 394

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 11.4 C C C 11.2 11.6 12.4 10.8 11.9 12.1 C 11.6 10.8 C 10.8 10.3

4527 14.5 C C 14.7 15.4 C 14.1 15.2 15.7 D 13.5 14.3 13.4 14.2 14.2

4528 10.9 9.9 11. 4 10.0 14.1 C C M 9.5 10.4 C C 10.9 11.2 M 11.1 M

4529 12.1 C C 12.8 12.9 13.3 12.1 11.9 12.6 11.3 10.7 11.6 12.0

4530 NOT PREGNA NT

4531 12.1 11.9 12.6 11.3 C 13.2 12.3 11.7 12.0 12.6 11.9 C C 11.8

4532 10.3 D 11.0 11.9 11.0 11.6 8.5 11.4 D 10.5 11.5 D 8.6 D 7.4 D D D

4533 14.4 15.4 14.0 14.1 13.8 14.7 15.0 C 14.0 C C C 14.1 13.3 15.6 C

4534 12.6 11.8 13.4 14.5 C C 14.3 C 12.0 C C C 12.9 11.9 11.6 10.6 13.1 C

4535 14.9 15.8 16.2 14.9 16.0 13.9 12.0 15.2 15.4 14.5

4536 11.9 11.5 13.1 12.5 11.9 C 10.0 C 11.6 11.8 11.8 D C 13.0 11.4 C

4537 13.5 12.2 15.7 12.8 12.2 13.0 12.6 13.0 15.9 C 12.6 14.8

4538 14.3 D 16.0 14.6 15.3 13.8 15.2 C C C C C 14.6 12.5 12.0 15.6 13.8

4539 NOT PREGNANT

4540 13.5 C 14.1 14.2 12.5 14.4 13.6 13.6 12.8 12.8 13.9 13.1 C

4541 NOT PREG., NO MATING DATE

4542 12.3 12.0 10.4 14.2 13.4 12.6 11.7 C C 12.7 12.2 12.1 11.4

4543 13.7 14.2 14.2 C 13.6 13.8 13.7 14.4 13.6 C 12.4 13.7 13.5 C C C

4544 14.1 14.9 C 14.4 14.8 13.8 C C 15.2 14.9 12.6 14.4 C C 13.6 12.4

4545 14.1 13.6 14.7 15.1 C 14.9 C C 14.3 13.4 14.8 12.9 13. 5 13.6

4546 13.7 14.2 C C 14.2 C C 14.0 13.5 14.8 C 13.0 13.7 C 12.9 13.6 13.1

4547 12.6 12.5 12.8 12.8 12.4 12.3 C 12.8 C C C 1 2.7 12.9 12.9 11.4

4548 14.8 16.6 12.7 14.8 14.9 14.9 C C 14.3 14.8 15.2 15.7 C 13.6 C C

4549 FOUND DEAD

4550 14.3 D C 13.8 15.2 15.3 C 15.3 15.0 13.9 15.0 C 13.1 C 13.4 12.9 C

4551 13.4 14.6 14.5 13.9 13.1 13.4 13.5 C 11.6 C 13.2 13.3 12.8

MEAN 13.1

S.D. 1.30

N 22

PUP STATUS CODES: D

- DIED C

- CULLED M

- MISSING23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 395

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 24.6 S 25.6 24.1 25.6 25.2 24.4 C C C 24.3 23.8 24.1 24.5 24.3 C

1527 DIED POST PARTUM

1528 23.7 C 23.1 21.6 25.4 C C 23.9 24.3 C 24.9 24.2 21.9 C 22.3 25.9

1529 27.2 29.1 27.0 28.0 C C C 26.3 C 28.0 25.7 26.2 28.2 25.2 28.4 C

1530 24.9 25.0 24.7 24.4 D 22.1 27.2 26.0 C 23.4 26.2 24.9 25.5

1531 25.4 C 25.1 C 25.2 28.7 26.5 23.5 22.6 26.9 C C 25.1 23.8 C C 26.2

1532 NOT PREGNANT

1533 24.0 24.6 C 24.4 23.2 C C 25.9 24.8 23.9 23.3 C 22.4 23.7 23.9 C C

1534 26.0 26.6 26.5 25.8 27.6 C 26.7 C C 25.5 25.8 23.7 25.0 26.3

1535 NOT PREGNANT

1536 16.1 C 17.8 15.5 20.9 16.2 12.7 C 12.9 16.9 15.3 15.3 17.8 C C C

1537 24.4 C 25.8 24.3 25.9 25.0 22.5 25.1 C C 23.6 23.7 24.5 23.3

1538 27.0 28.6 27.4 27.8 27.7 27.2 26.8 25.0 27.2 C 26.7 25.4 C

1539 25.9 C C 24.9 24.0 24.7 23.7 30.0 25.4 29.5 23.3 C 28.8 24.3

1540 28.5 28.7 31.4 28.5 28.7 27 .6 C 29.5 C 25.8 C C C 27.7 27.5 29.4

1541 NOT PREGNANT

1542 22.6 C 20.6 26.2 23.5 24.2 25.8 22.3 22.8 13.0 25.1 C C 22.4 C

1543 28.8 C 29.7 29.9 30.0 C C C 31.6 30.2 28.6 27.6 C C 25.9 25.9 28.6

1544 31.7 C 31.2 32.5 32.2 36.1 C C 31.9 31.4 32.8 30.5 26.7 C C D

1545 26.9 28.5 27.0 29.9 C C C 27.1 26.3 C 28.3 25.4 26.7 C 25.3 24.9

1546 22.3 22 .1 23.0 21.0 24.1 C C 23.5 C C 21.6 23.7 20.7 21.2 22.0

1547 22.5 22.1 22.1 24.4 C 22.7 22.2 C 23.1 22.0 C C 22.8 21.6 21.9

1548 NOT PREG., NO MATING DATE

1549 23.1 19.3 22.5 22.3 22.4 24.3 C 24.9 C 23.1 24.0 25.0 C 22.9

1550 NOT PREG., NO MATING DATE

1551 25.6 28.1 26.1 27.0 23.2 25.6 25.5 C 26.1 24.8 C C 24.4 C C 24.9

MEAN 25.1

S.D. 3.17

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 396

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 25.5 S D M 26.2 27.2 25.3 26.4 C 24.0 24.9 24.2 25.9 25.5

2527 NOT PREG., NO MATING DAT E

2528 25.5 S 25.1 28.2 25.8 C 24.6 26.2 C C 24.1 25.7 C 25.8 24.0 25.8

2529 23.3 24.0 2 3.7 23.5 22.4 23.2 C 22.7 22.5 23.2 23.0 C 24.9

2530 29.9 C 33.1 30.1 31.3 27.5 C M C C 26.2 28.7 30.3 C 31.8 30.3

2531 22.4 24.3 C C C C 23.5 23.1 C 23.3 24.1 18.4 21.9 22.5 C 19.8 22.6

2532 NOT PREGNANT

2533 24.1 24.3 23.7 24.6 25.3 24.6 22.1 C C C C C 23.8 23.2 25.2 23.9

2534 22.4 23.4 22.7 21.6 23.2 20.5 22.7

2535 18.3 C C 19.4 18.0 C 20.5 C 18.3 19.1 15.8 C 19.0 16.1 18.2 18.3

2536 25.3 S 28.4 25.7 27.1 24.5 25.2 D C C 25.4 24.0 23.3 C C 26.8 23.0

2537 22.1 21.4 21.4 23.1 23.6 22.0 C 21.7 21.6 22.1 22.0 21.6

2538 NOT PREG., NO MATING DATE

2539 24.7 D 23.9 C 23.3 25.0 C 28.3 25.1 24.0 25.6 25.9 C 22.3 23.1

2540 24.4 Z 24.6 25.0 24.5 24.8 25.4 23.8 22.2 24.4 24.9 24.3

2541 28.5 27.6 28.7 29.4 29.4 29.7 C 27.0 28.3 29.2 C C 28.1 28.1

2542 NOT PREG., NO MATING DATE

2543 23.8 D 23.3 22.9 22.5 24.3 21.3 24.5 C 25.4 23.3 C C 25.1 25.0

2544 25.7 27.0 24.0 24.9 26.8

2545 23.1 23.7 22.6 24.5 24.2 23.4 C C 20.7 23.2 23.5 21.8 23.2

2546 25.1 S 25.9 26.0 C 27.1 26.5 26.7 24.4 23.5 C 23.8 2 4.0 23.3

2547 21.4 21.5 21.2 22.1 C 21.3 22.0 21.2 21.1 21.5 20.6 21.8 C C

2548 25.7 25.9 C 26.4 24.0 26.3 24.7 28.0 27.0 25.2 25.1 24.4

2549 TOTAL LITTER LOSS

2550 28.5 30.7 27.0 27.3 29.2 29.1 27.8 29.5 27.4

2551 26.5 29.2 26.0 27.3 27.2 M C C 26.4 25.2 26.4 26.7 26.4 C 24.4 C

MEAN 24.6

S.D. 2.64

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING Z

- CANNIBALIZED23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 397

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 100 00 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREGNANT

3527 25.4 27.8 C 25.9 C 29.2 26.1 17.5 C 25.9 25.6 C C C C 23.9 C 25.3 26.3

3528 19.6 D 19.6 18.6 20.5 D 20.0 20.3 20.7 18.2 M M 18.9

3529 22.8 21.3 23.3 23.0 22.9 C C 22.0 C 21.0 24.7 C 23.9 22.0 24.4

3530 24.3 24.4 23.4 C 24.7 25.3 25.0 24.2 C 25.2 23.5 23.1 C 24.3

3531 24.5 23.6 24.9 C C 23.8 C 25.1 25.1 C 25.0 24.3 24.9 24.0 C 23.8

3532 24.4 25.9 20.6 26.2 24.4 25.7 24.8 C C 24.3 C 23.8 23.7 24.8

3533 26.3 S 25.2 26.9 C 27.9 C 25.9 27.3 26.1 25 .3 26.4 26.0 C 26.2

3534 24.3 25.1 C C 24.9 23.9 25.0 24.5 23.2 25.0 24.6 D C 24.2 22.1 C C

3535 22.2 21.9 24.4 C 25.5 23.1 21.2 23.4 21.4 C C 19.1 20.5 21.6 C C

3536 TOTAL LITTER LOSS

3537 20.7 20.6 C C 20.1 22.3 C C 19.1 21.6 21.7 C 20.5 C 20.6 C 18.5 21.7

3538 NOT PREG., NO MATING DATE

3539 NOT PREG., NO MATING DATE

3540 22.1 21.5 24.6 23.4 23.0 19.0 21.4 C C C 22.0 22.9 21.8 C 21.4

3541 24.9 S 26.8 24.0 24.6 25.0 25.4 22.8 25.8 C C 25.1 24.0 25.2

3542 26.7 26.6 26.3 C 29.0 26.4 C C C 28.2 27.5 26.4 24.6 26.4 25.4

3543 22.7 23.0 22.8 23.3 21.5 23.7 23.0 22.7 22.5 C 21.0 C C 23.7

3544 23.3 26.1 26.3 24.8 25.2 23.4 C 10.7 23.9 23.9 25.1 C C 23.2

3545 23.6 22.3 23 .2 26.6 D C C 26.4 C 24.2 C 22.3 22.5 20.6 22.2 C 25.4

3546 26.4 27.6 26.3 26.0 26.1 28.4 C C 25.0 C 25.2 27.1 C 26.4 C 25.7

3547 24.1 S Z 24.3 22.7 24.7 C 23.7 23.6 25.1 24.0 23.7 C 25.6 23.7 C

3548 TOTAL LITTER LOSS

3549 24.5 C C 25.2 25.2 24.2 24.9 23.2 C C 24.9 24.7 23.4 25.5 23.4

3550 NOT PREG., NO MATING DATE

3551 33.7 33.6 34.0 34.5 33.0 34.1 33.1

MEAN 24.3

S.D. 2.86

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED M

- MISSING23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 398

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPR ODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 19.2 C C C 19. 7 19.1 21.1 18.0 19.9 19.7 C 19.9 18.5 C 18.9 17.1

4527 23.2 C C 24.1 23.6 C 22.8 24.2 25.6 D 22.1 23.2 21.6 22.6 22.5

4528 18.8 17.5 20.0 1 7.6 22.6 C C M 15.0 17.2 C C 19.1 19.9 M 20.1 M

4529 20.4 C C 22.2 21.1 22.3 19.4 19.8 21.9 19.2 18.0 19.2 20.7

4530 NOT PREGNANT

4531 19.5 19.6 19.4 18.7 C 20.7 19.8 19.9 19.1 19.3 19.7 C C 18.9

4532 21.0 D 22.3 22.5 21.4 23.8 17.9 21.8 D 21.9 23.3 D 19.3 D 16.0 D D D

4533 25.5 26.5 24.8 26.3 23.8 24.5 27.9 C 25.2 C C C 26.0 23.1 27.4 C

453 4 24.0 23.3 25.6 25.9 C C 27.2 C 24.1 C C C 23.5 22.0 22.6 21.8 24.4 C

4535 22.7 24.8 24.9 22.3 23.0 20.8 19.0 24.1 22.3 22.7

4536 22.8 22.6 23.5 23.9 22.5 C 20.7 C 22.8 22.2 22.9 D C 24.4 22.8 C

4537 25.6 23.7 29.0 23.9 24.0 24.3 23.4 24.8 30.7 C 23.4 28.8

4538 24.3 D 26.0 24.5 25.2 22.4 25.2 C C C C C 25.3 22.6 21.4 25.7 24.4

4539 NOT PREGNANT

4540 20.6 C 20.7 21.3 20.3 21.7 20.4 20.4 20.2 19.9 21.1 19.7 C

4541 NOT PREG., NO MATING DATE

4542 22.3 23.4 19.6 23.5 24.2 22.7 22.8 C C 22.6 22.0 21.5 21.0

4543 23.2 22.7 24.9 C 24.2 22.2 23.1 24.8 22.7 C 21.9 23.3 22.4 C C C

4544 23.5 24.5 C 23.8 23.8 22.9 C C 24.5 25.0 22.0 23.1 C C 23.0 22.4

4545 24.2 23.9 24.9 25.1 C 26.5 C C 23.6 23.4 24.4 22.2 24.0 2 3.7

4546 24.4 24.0 C C 24.8 C C 25.8 23.4 25.5 C 24.2 24.7 C 23.3 23.5 25.0

4547 22.1 23.3 22.6 21.8 21.6 22.9 C 19.8 C C C 20.4 23.2 23.3 22.4

4548 26.0 28.2 23.9 25.8 26.5 27.0 C C 24.0 25.9 27.3 26.1 C 25.7 C C

4549 FOUND DEAD

4550 24.1 D C 24.0 25.3 25.7 C 24.6 25.0 23.6 25.9 C 22.7 C 22.2 21.7 C

4551 22.1 22.2 22.9 23.0 23.1 21.9 22.4 C 20.7 C 22.3 21.4 21.6

MEAN 22.7

S.D. 2.09

N 22

PUP STATUS CODES: D

- DIED C

- CULLED M

- MISSING23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 399

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 6 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 41.0 S 43.3 36.6 43.6 43.0 40.8 C C C 40.4 41.3 40.4 40.1 40.5 C

1527 DIED POST PARTUM

1528 35.0 C 32.8 33.1 39.0 C C 34.9 35.5 C 37.3 33.3 31.7 C 33.5 39.2

1529 42.7 44.4 43.0 46.9 C C C 41.0 C 42.2 39.1 42.7 45.1 36.6 45.5 C

1530 38.4 37.4 40.4 36.7 D 33.7 43.5 40.8 C 35.7 38.2 38.1 39.4

1531 40.0 C 37.0 C 39.8 43.0 41.5 38.8 37.2 42.7 C C 41.7 35.7 C C 42.9

1532 NOT PREGNANT

1533 38.6 37.1 C 38.3 37.6 C C 43.3 39.5 37.9 36.9 C 35.2 40.0 39.9 C C

1534 43.9 48.3 45.3 44.2 47.3 C 42.4 C C 43.3 39.1 42.4 41.7 44.6

1535 NOT PREGNANT

1536 25.2 C 26.8 24.0 30.5 24.3 19.4 C 22.0 27.0 23.9 25.9 28.5 C C C

1537 39.2 C 42.5 36.6 37.6 41.5 42.5 33.3 C C 42.2 38.8 39.1 38.2

1538 41.9 44.2 38.0 44.2 43.8 41.9 41.9 38 .8 43.5 C 42.0 40.6 C

1539 40.1 C C 37.3 38.0 39.0 35.4 41.4 45.9 39.2 46.0 C 39.1 39.6

1540 46.2 49.3 52.4 49.1 45.9 39.9 C 49.3 C 41.3 C C C 47.2 43.0 44.5

1541 NOT PREGNANT

1542 36.4 C 32.6 43.2 38.8 37.4 42.3 36.4 36.3 17.5 42.9 C C 36.4 C

1543 49.5 C 49.7 52.6 51.6 C C C 53.5 52.1 50.9 48.1 C C 45.2 44.0 47.6

1544 48.8 C 48.8 52 .2 51.4 54.4 C C 49.4 48.4 48.9 46.3 39.8 C C D

1545 44.5 48.4 45.3 48.2 C C C 48.7 41.5 C 45.4 42.6 43.2 C 40.4 40.8

1546 36.4 34.8 37.6 32.4 40.1 C C 39.8 C C 36.8 39.7 32.7 34.2 36.0

1547 38.7 37.7 38.4 42.9 C 38.7 39.7 C 36.6 36.0 C C 41.2 37.8 38.0

1548 NOT PRE G., NO MATING DATE

1549 37.2 29.1 34.1 34.4 35.4 39.0 C 42.3 C 38.7 40.8 41.8 C 36.1

1550 NOT PREG., NO MATING DATE

1551 40.5 41.3 46.0 43.7 37.1 40.8 42.7 C 43.2 37.1 C C 39.3 C C 33.3

MEAN 40.2

S.D. 5.32

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 400

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 41.4 S D M 41.0 42.2 44.5 44.5 C 37.7 39.9 39.5 41.8 41.3

2527 NOT PREG., NO MATING DATE

2528 41.1 S 41.9 43.7 42.8 C 41.4 39.8 C C 36.2 39.6 C 42.0 39.5 43.7

2529 38.5 38.4 37.9 38.8 36.3 38.3 C 39.1 37.0 37.9 38.6 C 42.4

2530 48.7 C 53.6 49.2 43.1 45.0 C M C C 52.5 46.2 47.8 C 49.3 51.6

2531 38.3 41. 5 C C C C 39.7 39.6 C 38.7 43.3 32.1 36.1 40.2 C 33.9 38.2

2532 NOT PREGNANT

2533 40 .3 40.5 38.4 41.9 39.5 41.4 39.3 C C C C C 39.9 39.4 41.9 40.6

2534 37.6 36.8 37.8 36.2 40.1 34.0 40.8

2 535 29.0 C C 31.1 27.8 C 32.0 C 27.0 29.8 26.8 C 30.5 25.6 29.0 30.8

2536 39.8 S 42.8 40.2 44.6 37.5 39.6 D C C 42.5 37.4 35.3 C C 40.4 37.4

2537 39.2 38.0 35.6 40.4 40.6 38.7 C 38.6 40.9 39.3 39.9 39.8

2538 NOT PREG., NO MATING DATE

2539 41.0 D 37.4 C 39.0 43.7 C 46.3 44.2 40.2 43.5 41.9 C 35.9 38.3

2540 41.0 Z 43.5 41.4 40.7 42.4 43.5 38.9 35.8 42.2 41.1 40.0

2541 46.4 43.2 49.0 42.2 49.8 52.0 C 45.0 44.2 48.2 C C 44.7 46.0

2542 NOT PREG., NO MATING DATE

2543 40.3 D 40.0 37.2 40.3 40.7 35.6 41.9 C 42.3 39.4 C C 42.0 43.3

2544 44.1 47.3 42.2 40.6 46.4

2545 39.5 41.8 36.7 41.8 40.9 41.1 C C 35.7 42.6 40.4 36.6 37.2

2546 42.7 S 42.7 42.3 C 45.9 46.1 45.4 40.8 42.2 C 41.4 40.7 39.5

2547 39.0 38.8 38.9 40.5 C 38.8 40.6 38.6 39.4 40.9 36.5 37.5 C C

2548 43.3 44.3 C 44.1 40.1 46.2 41.9 47.1 44.1 41.3 43.4 40.8

2549 TOTAL LITTER LOSS

2550 49.6 51.2 48.0 50.7 51.3 50.0 48.2 50.5 46.9

2551 46.2 50.9 46.1 48.0 47.3 M C C 44.6 45.4 46.0 46.3 46.1 C 41.4 C

MEAN 41.3

S.D. 4.40

N 21

PUP STATUS CODE S: S

- STILLBORN D

- DIED M

- MISSING Z

- CANNIBALIZED23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 401

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 M G/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# ME AN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-------------------------------------------------------------------------------------------------------------- ---------------------

3526 NOT PREGNANT

3527 45.6 48.6 C 45.9 C 49.3 45.8 46.5 C 44.7 43.8 C C C C 41.3 C 43.7 46.7

3528 31.7 D 30.0 29.0 34.3 D 32.9 33.0 34.3 29.8 M M 29.9

3529 36.2 33.2 36.6 35.6 37.7 C C 34.8 C 33.1 39.8 C 39.1 35.7 36.0

3530 40.2 43.5 41.0 C 35.8 38.9 41.0 42.7 C 41.1 39.6 36.9 C 41.5

3531 42.3 42.0 42.1 C C 39.7 C 42.6 43.0 C 46.9 41.0 43 .1 42.4 C 39.8

3532 39.5 39.5 33.2 42.5 40.1 41.1 39.4 C C 39.5 C 39.9 37.2 42.4

3533 45.9 S 43.8 46.8 C 48.0 C 44.3 46.9 46.7 45.1 47.0 45.7 C 45.0

3534 42.4 40.6 C C 44.4 43.5 44.8 41.3 40.8 44.5 42.3 D C 44.1 38.0 C C

3535 37.2 36.2 36.7 C 43.2 39.9 36.7 39.6 37.5 C C 33.1 32.1 36.8 C C

3536 TOTAL LITTER LOSS

3537 33.2 29.9 C C 33.2 33.8 C C 32 .9 36.7 34.2 C 32.9 C 32.7 C 28.7 36.6

3538 NOT PREG., NO MATING DATE

3539 NOT PREG., NO MATING DATE

3540 33.9 31.6 36.9 35.6 35.2 29.5 31.5 C C C 35.0 37.4 32.2 C 33.8

3541 41.1 S 43.1 38.2 39.6 42.8 42.9 38.7 42.3 C C 41.0 40.1 42.2

3542 47.5 47.5 47.9 C 50.5 48.0 C C C 48.7 48.1 45.7 43.1 49.0 46.5

3543 36.5 36.6 31.6 39.4 37 .6 38.3 34.4 37.0 36.8 C 33.8 C C 39.0

3544 37.2 41.1 40.5 41.7 39.3 38.0 C 16.8 39.4 37.0 39.5 C C 38.3

3545 38.4 37.9 36.0 45.3 D C C 41.7 C 38.8 C 34.4 39.6 33.6 35.4 C 41.6

3546 44.4 45.6 45.5 44.5 42.4 47.5 C C 41.0 C 44.0 44.6 C 46.5 C 42.1

3547 39.7 S Z 40.6 36.9 41.1 C 39.0 38.2 38.9 41.5 39.7 C 43.4 37.8 C

3548 TOTAL LITTER LOSS

3549 41. 3 C C 42.0 43.0 41.1 43.3 39.5 C C 41.6 40.4 39.5 42.9 40.1

3550 NOT PREG., NO MATING DATE

35 51 58.5 59.2 62.6 61.0 53.1 57.8 57.3

MEAN 40.6

S.D. 6.03

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED M

- MISSING23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 402

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUC TION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 32.3 C C C 34.9 3 2.6 34.9 29.1 33.1 35.3 C 33.2 30.5 C 31.1 28.6

4527 37.4 C C 39.2 39.0 C 35.0 38.2 40.3 D 36.0 35.4 36.3 38.9 35.6

4528 32.0 30.0 34.2 29.6 38.4 C C M 28.3 30.1 C C 32.1 32.5 M 32.6 M

4529 31.8 C C 35.4 32.6 36.8 28.5 29.8 32.7 30.1 27.7 30.6 33.4

4530 NOT PREGNANT

4531 33.0 32.5 32.1 30.9 C 34.0 33.0 35.2 33.9 33.2 33.6 C C 32.0

4532 35.0 D 37.5 37.6 34.6 39.8 30.0 35.1 D 36.2 40.3 D 32.9 D 25.7 D D D

4533 43.9 45.5 44.3 43.1 40.1 43.7 47.4 C 42.5 C C C 44.2 41.9 46.0 C

4534 38.1 37.7 40.4 39.1 C C 42.3 C 38.0 C C C 39.1 34.2 34.4 35.1 40.3 C

4535 37.2 43.0 39.9 36.0 35.0 33.1 29.7 42.6 36.4 39.0

4536 38.5 37.6 41.2 39.3 38.1 C 33.7 C 39.1 38.3 38.3 D C 41.1 37.8 C

4537 42.9 38.4 49.8 40.0 40.5 40.3 40.0 40.3 51.5 C 39.6 48.5

4538 39.1 D 42.3 38.5 39.6 37.6 41.3 C C C C C 41.8 35.3 33.8 41.8 38.7

4539 NOT PREGNANT

4540 33.4 C 31.7 32.9 33.2 35.6 33.8 34.2 31.3 31.4 35.9 33.9 C

4541 NOT PREG., NO MATING DATE

4542 37.5 38.3 31.6 40.1 41.0 37.2 36.8 C C 38.2 37.3 36.7 37.8

4543 39.7 40.1 43.0 C 40.1 36.8 39.1 43.5 36.7 C 38.3 38.9 40.8 C C C

4544 41.4 44.4 C 42.3 39.2 42.1 C C 43.6 42.7 38.3 41.6 C C 42.1 38.1

4545 39.8 40.1 38.6 40.2 C 42.8 C C 36.5 38.6 42.5 37.5 38.6 43.0

4546 41.9 42.5 C C 42.8 C C 44.4 40.3 43.0 C 40.5 42.3 C 40.0 40.5 42.6

4547 36.6 38.4 36.3 36.5 35.3 37.2 C 33.4 C C C 35.5 40.2 36.7 36.8

4548 43.7 46.2 38.5 43.6 44.1 45.2 C C 42.4 42.0 46.9 44.4 C 43.3 C C

4549 FOUND DEAD

4550 42.0 D C 43.1 45.0 43.1 C 41.9 42.4 40.8 46.8 C 40.0 C 39.7 37.3 C

4551 35.9 35.9 35.9 32.8 36.4 37.4 40.3 C 32.0 C 36.0 38.4 33.9

MEAN 37.9

S.D. 3.85

N 22

PUP STATUS CODES: D

- DIED C

- CULLED M

- MISSING23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 403

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 74.8 S 81.1 67.6 81.2 80.5 77.8 C C C 73.9 70.7 73.3 71.3 70.6 C

1527 DIED POST PARTUM

1528 72.7 C 73.5 68.1 79.6 C C 75.3 76.9 C 73.8 70.5 66.4 C 69.3 73.2

1529 82.0 84.3 82.3 91.9 C C C 84.8 C 85.0 71.7 78.2 80.8 75.1 86.3 C

1530 72.3 73.2 76.2 71.1 D 64.9 81.4 75.5 C 67.6 72.4 69.7 71.3

1531 75.9 C 77.3 C 81.7 83.5 80.5 70.5 67.5 78.6 C C 75.3 67.8 C C 75.9

1532 NOT PREGNANT

1533 75.7 78.9 C 80.8 77.5 C C 85.3 76.5 70.3 69.7 C 69.0 76.6 72.8 C C

1534 78.2 84.9 80.8 80.9 85.6 C 79.3 C C 74.8 72.6 71.5 74.0 78.0

1535 NOT PREGNANT

1536 49.5 C 52.8 48.7 58.9 51.9 38.9 C 42.8 53 .9 44.9 50.2 52.3 C C C

1537 72.1 C 73.6 71.3 72.2 75.6 75.0 65.5 C C 79.1 69.4 70.1 69.1

1538 80.2 87.7 84.6 85.1 86.6 81.1 77.5 72.8 77.8 C 74.3 74.4 C

1539 74.7 C C 70.8 72.0 73.1 71.0 85.6 71.8 78.8 69.9 C 84.5 69.5

1540 87.4 94.1 98.9 95.2 91.4 78.5 C 89.7 C 81.9 C C C 82.5 81.9 80.2

1541 NOT PREGNANT

1542 71.3 C 63.8 83.9 79.0 72 .9 81.6 69.6 71.0 38.3 82.2 C C 70.6 C

1543 89.5 C 96.8 95.1 100.3 C C C 98.0 97.6 85.6 83.4 C C 76.1 77.9 84.2

1544 87.6 C 86.2 95.8 93.6 104.5 C C 93.7 81.4 82.4 81.4 69.6 C C D

1545 81.5 87.6 81.6 92.4 C C C 87.0 78.6 C 83.4 77.2 79.0 C 70.5 77.6

1546 72.1 70.7 74.2 70.2 79.4 C C 75.5 C C 70.5 77.3 65.6 66.6 71.3

1547 75.2 72.5 76.9 84.6 C 75.8 78.1 C 76.4 72.3 C C 71.0 73.7 70.9

1548 NOT PREG., NO MATING DATE

1549 73.4 63.9 69.1 72.9 74.0 75.2 C 84.1 C 74.3 73.8 75.0 C 72.0

1550 NOT PREG., NO MATING DATE

1551 79.8 88.0 89.4 81.4 76.5 87.5 80.0 C 82.9 72.5 C C 66.1 C C 73.7

MEA N 76.3

S.D. 8.39

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 404

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 77.1 S D M 79.0 80.2 85.7 80.6 C 71.7 70.9 73.2 74.8 77.9

2527 NOT PREG., NO MATING DATE

2528 79.7 S 82.3 86.3 83.5 C 84.2 84.6 C C 75.3 69.9 C 77.8 74.3 78.5

2529 73.5 76.5 74.3 72. 4 72.5 72.6 C 78.9 67.1 69.1 74.8 C 77.1

2530 89.2 C 102.3 91.4 91.3 82.9 C M C C 89.0 89.0 84.4 C 79.6 92.6

2531 73.2 78.0 C C C C 78.7 78.9 C 73.3 82.6 65.2 70.5 71.4 C 62.0 71.3

2532 NOT PREGNANT

2533 75.2 79.5 74.4 77.3 75.6 74.9 71.2 C C C C C 68.7 74.0 81.2 74.8

2534 70.6 76.2 72.6 64.2 72.7 63.2 74.9

2535 62.5 C C 65.9 62.4 C 66.0 C 60.2 71.2 59.1 C 62.2 54.7 61.4 62.3

2536 79.5 S 88.5 83.0 92.3 78.7 74.7 D C C 82.7 71.5 69.8 C C 78.2 75.5

2537 73.5 75.7 71.3 78.1 76.3 75.3 C 70.7 75.4 70.6 72.5 69.0

2538 NOT PREG., NO MATING DATE

2539 76.4 D 73.0 C 76.0 85.5 C 87.8 81.2 73.8 73.4 79.0 C 64.7 69.8

2540 78.7 Z 82.9 81.2 80.4 81.8 86.9 76.9 68.8 75.2 77.4 75.5

2541 85.6 86.4 91.8 84.8 88.8 93.2 C 80.6 81.1 84.5 C C 82.2 82.4

2542 NOT PREG., NO MATING DATE

2543 72.6 D 80.2 69.5 72.4 71.7 64.1 75.1 C 74.6 69.1 C C 71.3 77.7

2544 83.1 91.1 83.1 76.0 82.1

2545 74.7 79.5 73.7 83.4 77.2 78.1 C C 68.8 73.5 73.3 68.9 70.3

2546 79.7 S 79.3 84.9 C 85.5 89.3 86.6 75.4 78.0 C 74.0 75.9 67. 7

2547 75.0 76.8 75.4 77.8 C 78.7 75.9 70.3 78.3 78.0 67.7 71.5 C C

2548 79.0 83.1 C 83.3 77.8 87.3 77.5 80.5 78.5 73.0 75.3 73.5

2549 TOTAL LITTER LOSS

2550 87.2 94.3 85.9 92.4 92.4 86.2 80.9 82.6 82.9

2551 84.1 96.3 86.3 87.2 88.8 M C C 80.0 77.6 86.4 81.9 82.6 C 74.0 C

MEAN 77.6

S.D. 6.14

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING Z

- CANNIBALIZED23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 405

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

------------------------------------------------------------------------------------------------------------------ -----------------

3526 NOT PREGNANT

3527 82.8 92.5 C 89.4 C 94.0 83.4 85.9 C 75.9 74.7 C C C C 72.4 C 78.4 81.1

3528 66.0 D 68.1 64.0 71.6 D 65.0 68.8 69.1 59.4 M M 61.8

3529 73.7 69.7 77.7 78.1 78.0 C C 70.5 C 67.5 80.8 C 76.9 70.8 67 .1

3530 75.1 81.5 79.1 C 69.7 76.8 77.4 74.7 C 71.9 74.3 69.4 C 76.4

3531 77.4 76.1 77.6 C C 77.2 C 77.4 82.2 C 84.9 74.3 78.0 75.5 C 70.7

3532 75.5 80.2 67.1 83.9 76.2 74.6 78.0 C C 74.6 C 72.4 68.9 78.7

3533 86.5 S 86.7 89.5 C 91.8 C 83.1 89.5 85.8 81.2 86.3 86.8 C 84.4

3534 77.3 76.7 C C 82.4 79.4 82.3 77.5 76.5 75.3 75.7 D C 81.7 65.8 C C

3535 70.1 73.3 75.8 C 75.8 72.1 69.9 69.3 70.0 C C 61.1 63.3 70.5 C C

3536 TOTAL LITTER LOSS

3537 65.5 60.7 C C 65.6 64.3 C C 64.2 71.3 70.7 C 63.3 C 66.0 C 58.1 70.7

3538 NOT PREG., NO MATING DATE

3539 NOT PREG., NO MATING DATE

3540 63.6 62.0 70.9 67.7 67.4 57.1 58.4 C C C 62.9 68.9 59.5 C 61.5

3541 74.6 S 81.9 70.5 72.4 79.2 79.1 67.8 74.8 C C 72.4 72.4 75.6

3542 82.9 83.5 87.0 C 88.7 86.8 C C C 86.7 85.8 77.0 73.6 80.4 79.2

3543 69.6 72.1 64.1 78.9 69.4 74.6 69.3 68.3 64.6 C 63.1 C C 71.9

3544 70.9 81.4 79.6 78.7 76.8 76.2 C 32.8 71.0 68.8 72.1 C C 71.6

3545 71.5 70.8 76.9 79.1 D C C 79.2 C 73.5 C 68.6 64.2 66.1 64.9 C 72.0

3546 85.2 88.5 90.6 92.8 84.0 92.9 C C 75.4 C 82.2 81.8 C 86.4 C 77.6

3547 75.3 S Z 77.8 71.5 81.4 C 80.9 73.2 72.4 77.0 75.0 C 77.0 66.6 C

3548 TOTAL LITTER LOSS

3549 79.8 C C 84.9 83.6 82.5 85.5 79.7 C C 77.8 80.2 74.1 74.8 75.3

3550 NOT PREG., NO MATING DATE

3551 106.0 103.2 117.1 105.2 102.6 106.4 101.7

MEAN 76.5

S.D. 9.45

N 20

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED M

- MISSING23

- JUN

- 2009 19:46

Huntingdon Life Sciences 00

- 4206 PAGE 406

APPENDIX U

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 64.2 C C C 69.5 67.7 70.5 61.4 65.4 66.9 C 64.0 58.4 C 59.3 58.5

4527 72.1 C C 74.2 69.9 C 71.1 72.8 79.4 D 68.6 67.5 69.3 72.9 74.9

4528 64.7 62.6 67.5 61.2 75. 2 C C M 62.2 63.6 C C 63.8 63.6 M 62.4 M

4529 66.2 C C 72.8 70.4 74.2 63.1 62.8 69.1 63.4 57.7 61.4 67.2

4530 NOT PREGNANT

4531 65.2 64.9 65.8 61.4 C 70.1 67.9 66.4 66.5 63.7 64.6 C C 61.1

4532 68.5 D 73.1 72.5 71.2 78.9 59.5 70.4 D 71.6 73.2 D 61.9 D 52.3 D D D

4533 83.5 87.8 83.8 82.9 83.2 84.8 87.0 C 80.4 C C C 81.2 78.4 85.8 C

4534 71.2 72.4 76.2 77.6 C C 81.7 C 69.7 C C C 73.6 64.2 63.6 63.2 70.1 C

4535 70.7 81.4 78.3 70.9 69.4 62.8 59.6 78.5 67.5 68.2

453 6 71.7 71.4 77.1 75.5 73.4 C 65.9 C 72.9 67.3 71.1 D C 73.8 68.3 C

4537 78.1 72.5 87.3 74.3 76.0 76.1 72.4 73.9 89.2 C 73.0 85.8

4538 72.0 D 75.9 72.9 76.7 69.8 75.9 C C C C C 72.0 71.3 63.2 73.6 68.3

4539 NOT PREGNANT

4540 65.3 C 64.3 66.1 65.2 67.5 67.6 64.9 61.1 63.8 67.3 65.6 C

4541 NOT PREG., NO MATING DATE

4542 71.6 74.9 62.8 73.2 78.6 73.1 70.3 C C 76.6 68.6 70.9 67.0

4543 75.2 77.0 78.6 C 77.3 75.9 74.0 76.4 69.8 C 73.6 73.5 76.0 C C C

4544 75.5 81.6 C 79.4 74.9 75.7 C C 80.8 76.7 68.5 73.3 C C 73.5 70.7

4545 77.9 76.3 78.8 81.2 C 86.4 C C 76.2 76.7 81.6 72.9 72.7 75.8

4546 76.2 78.8 C C 79.5 C C 77.6 79.1 81.9 C 72.5 74.2 C 71.3 72.2 74.6

4547 69.3 74.7 69.0 72.6 72.7 72.2 C 63.2 C C C 64.9 69.8 67.9 65.6

4548 78.0 87.5 71.6 76.5 77.3 84.3 C C 73.7 76.3 81.6 75.4 C 75.3 C C

4549 FOUND DEAD

4550 77.7 D C 79.1 85.3 84.4 C 80.0 78.8 73.4 83.7 C 72.3 C 73.6 66.3 C

4551 67.9 69.7 67.4 67.4 65.7 69.7 73.6 C 62.8 C 66. 9 71.3 64.8

MEAN 71.9

S.D. 5.30

N 22

PUP STATUS CODES: D

- DIED C

- CULLED M

- MISSING23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 407

APPENDIX V

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL # O RGAN OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

1526 ESTROUS STAGE DIESTRUS

1527 DPP ESTROUS STAGE a

1528 ESTROUS STAGE DIESTRUS

1529 PNMD ESTROUS STAGE DIESTRUS

1530 ESTROUS STAGE DIESTRUS

1531 ESTROUS STAGE METESTRUS

1532 NP ESTROUS STAGE ESTRUS

1533 ESTROUS STAGE DIESTRUS

1534 ESTROUS STAGE ESTRUS

1535 NP ESTROUS STAGE DIESTRUS

1536 ESTROUS STAGE DIESTRUS

1537 ESTROUS STAGE DIESTRUS

1538 ESTROUS STAGE DIESTRUS

1539 ESTROUS STAGE DIESTRUS

1540 ESTROUS STAGE DIESTRUS

1541 NP ESTROUS STAGE ESTRUS

1542 ESTROUS STAGE DIESTRUS

1543 ESTROUS STAGE DIESTRUS

1544 ESTROUS STAGE DIESTRUS

1545 ES TROUS STAGE DIESTRUS

1546 ESTROUS STAGE DIESTRUS

1547 ESTROUS STAGE DIESTRUS

1548 NNMD ESTROUS STAGE DIESTRUS

1549 ESTROUS STAGE DIESTRUS

1550 NNMD ESTROUS STAGE DIESTRUS

1551 ESTROUS STAGE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PRE GNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

a No vaginal smear taken for this animal (found dead on Lactation Day 1).23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 408

APPENDIX V

GASOLI NE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL # O RGAN OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

2526 ESTROUS STAGE PROESTRUS

2527 NNMD ESTROUS STAGE ESTRUS

2528 ESTROUS STAGE DIESTRUS

2529 ESTROUS STAGE DIESTRUS

2530 ESTROUS STAGE PROESTRUS

2531 ESTROUS STAGE DIESTRUS

2532 NP ESTRO US STAGE ESTRUS

2533 ESTROUS STAGE DIESTRUS

2534 ESTROUS STAGE DIESTRUS

2535 ESTROUS STAGE DIESTRUS

2536 ESTROUS STAGE DIESTRUS

2537 ESTROUS STAGE DIESTRUS

2538 NNMD ESTROUS STAGE DIESTRUS

2539 ESTROUS STAGE DIESTRUS

2540 ESTROUS STAGE DIESTRUS

2541 ESTROUS STAGE DIESTRUS

2542 NNMD ESTROUS STAGE ESTRUS

2543 ESTROUS ST AGE DIESTRUS

2544 ESTROUS STAGE DIESTRUS

2545 ESTROUS STAGE ESTRUS

2546 ESTROUS STAGE ESTRUS

2547 ESTROUS STAGE DIESTRUS

2548 ESTROUS STAGE DIESTRUS

2549 TLL ESTROUS STAGE ESTRUS

2550 ESTROUS STAGE ESTRUS

2551 ESTROUS STAGE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DAT E TLL=TOTAL LITTER LOSS23

- JUN

- 2009 19:01

H untingdon Life Sciences 00

- 4206 PAGE 409

APPENDIX V

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL # O RGAN OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

3526 NP ESTROUS STAGE PROESTRUS

3527 ESTROUS STAGE DIESTRUS

3528 ESTROUS STAGE DIESTRUS

3529 ESTROUS STAGE ESTRUS

3530 ESTROUS STAGE DIESTRUS

3531 ESTROUS STAGE DIESTRUS

3532 ESTROUS STAGE DIESTRUS

3533 ESTROUS STAGE DIESTRUS

3534 ESTROUS STAGE DIESTRUS

3535 ESTROUS STAGE DIESTRUS

3536 TLL ESTROUS STAGE DIESTRUS

3537 ESTROUS STAGE DIESTRUS

3538 NNMD ESTROUS STAGE ESTRUS

3539 NNMD ESTROUS STAGE ESTRUS

3540 ESTROUS STAGE DIESTRUS

3541 ESTROUS STAGE DIESTRUS

3542 ESTROUS STAGE DIESTRUS

3543 ESTROUS STAGE METESTRUS

3544 ESTROUS STAGE DIESTRUS

3545 ESTROUS STAGE DIESTRUS

3546 ESTROUS STAGE DIESTRUS

3547 ESTROUS STAGE DIESTRUS

3548 TLL ESTROUS STAGE DIESTRUS

3549 ESTROUS STAGE DIESTRUS

3550 NNMD ESTROUS STAGE DIESTRUS

3551 ESTROUS STAGE PROESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=N OT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS23

- JUN

- 2009 19:01

Huntingdon Life Sciences 00

- 4206 PAGE 410

APPENDIX V

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTI ON TOXICITY STUDY IN RATS

INDIVIDUAL TERMINAL ESTROUS DETERMINATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL # O RGAN OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

4526 ESTROUS STAGE ESTRUS

4527 ESTROUS STAGE DIESTRUS

4528 ESTROUS STAGE DIESTRUS

4529 ESTROUS STAGE DIESTRUS

4530 ESTROUS STAGE DIESTRUS

4531 ESTROUS STAGE DIESTRUS

4532 ESTROUS STAGE DIESTRUS

4533 ESTROUS STAGE DIESTRUS

4534 ESTROUS STAGE DIESTRUS

4535 ESTROUS STAGE ESTRUS

4536 ESTROUS STAG E DIESTRUS

4537 ESTROUS STAGE DIESTRUS

4538 ESTROUS STAGE PROESTRUS

4539 NP ESTROUS STAGE DIESTRUS

4540 ESTROUS STAGE DIESTRUS

4541 NNMD ESTROUS STAGE ESTRUS

4542 ESTROUS STAGE DIESTRUS

4543 ESTROUS STAGE DIESTRUS

4544 ESTROUS STAGE DIESTRUS

4545 ESTROUS STAGE DIESTRUS

4546 ESTROUS STAGE DIESTRUS

4547 PNMD ESTROUS STAGE PROESTRUS

4548 ESTROUS STAGE DIESTRUS

4549 DIED ESTROUS STAGE b

4550 ESTROUS STAGE DIESTRUS

4551 ESTROUS STAGE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE

b Animal died before mating.23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 411

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1026 NO REMARKABLE OBSERVATIONS

1027 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

1028 ABDOMINAL CAVITY DISCOLORED LYMPH NODE(S); MODERATE; RIGHT

PERI

- RENAL, RED

TISSUES SAVED

1029 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

1030 NO REMARKABLE OBSERVATIONS

1031 NO REMARKABLE OBSERVATIONS

1032 NO REMARKABLE OBSERVATIONS

1033 NO REMARKABLE OBSERVATIONS

1034 NO REMARKABLE OBSERVATIONS

1035 NO REMARKABLE OBSERVATIONS

1036 NO REMARKABLE OBSERVATIONS

103 7 NO REMARKABLE OBSERVATIONS

1038 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

1039 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

AND DIAPHRAGMATIC LOBES - 0.1 CM IN DIAMETER, TAN

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 412

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOL E- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL # ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1040 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

AND DIAPHRAGMATIC LOBE, TAN, 0.1 CM IN DIAMETER

KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM ALOPECIA GENERAL

EXTERNAL FINDING NOT CONFIRMED

1041 NO REMARKABLE OBSERVATIONS

1042 GONADS TESTE(S) SMALL; SLIGHT; LEFT

GONADS TESTE(S) SOFT; MODERATE; LEFT

GONADS EPIDIDYMIS(IDES) SMALL; SLIGHT; LEFT

1043 THYMUS DISCOLORED FOCI; SLIGHT; LEFT

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

1044 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

1045 NO REMARKABLE OBSERVATIONS

1046 NO REMARKABLE OBSERVATIONS

1047 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

EXTERNAL EXAM ALOPECIA - E XTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

1048 KIDNEY IRREGULAR SURFACE; SLIGH T; LEFT

ROUGH

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 413

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY S TUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1048 (CONTINUED)

EXTERNAL EXAM INCISORS BRO KEN/MISSING

EXTERNAL FINDING CONFIRMED

1049 NO REMARKABLE OBSERVATIONS

1050 NO REMARKABLE OBSERVATIONS

1051 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 414

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2026 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

2027 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

EXTERNAL EXAM ALOPEC IA - EXTREMITIES/SNOUT; SLIGHT

EXTERNAL FINDINGS CONFIRMED

2028 NO REMARKABLE OBSERVATIONS

2029 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, RED, LESS THEN 0.1 CM IN DIAMETER,

2030 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - 0.1 CM IN DIAMETER, TAN

BLADDER CALCULI; MODERATE

WHITE, 0.5 CM IN DIAMETER, FIRM

TISSUE SAVED

2031 THYMUS DISCOLORED FOCI; EXTREME; BILATERAL

RED, 0.2 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1

- 0.2 CM IN DIAMETER

KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

2032 THYMUS DISCOLORED FOCI; MODERATE; RIGHT

LESS THAN 0.1 CM IN DIAMETER, RED

TISSUE SAVED

EXTERNAL EXAM INCISOR S BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

2033 NO REMARKABLE OBS ERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 415

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECRO PSY OBSERVATIONS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2034 NO REMARKABLE OBSERVATIONS

2035 NO REMARKABLE OBSERVATIONS

2036 NO REMARKABLE OBSERVATIONS

2037 NO REMARKABLE OBSERVATIONS

2038 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

2039 NO REMARKABLE OBSERVATIONS

2040 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER

2041 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES - 0.1 CM IN DIAMETER, TAN

EXTERNAL EXAM INCISORS MALOCC LUDED

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

2042 THYMUS DISCOLORED FOCI; EXTREME; LEFT

RED, 0.1

- 0.2 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

2043 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 416

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2044 NO REMARKABLE OBSERVATIONS

2045 NO REMARKABLE OBSERVATIONS

2046 NO REMARKABLE OBSERVATIONS

2047 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN

2048 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

2049 NO REMARKABLE OBSERVATIONS

2050 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

2051 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 417

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3026 NO REMARKABLE OBSERVATIONS

3027 KIDNEY DILATED RENAL PELVIS; EXTREME; RIGHT

3028 LUNGS DISCOLORED FOCI; SLIGHT

RIGHT DIAPHRAGMATIC LOBE, TAN, 0.1 CM IN DIAMETER

KIDNEY DILATED RENAL PELVIS; SLIGHT; BILATERAL

3029 NO REMARKABLE OBSERVATIONS

3030 NO REMARKABLE OBSERVATIONS

3031 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER

3032 NO REMARKABLE OBSERVATIONS

3033 NO REMARKABLE OBSERVATIONS

3034 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

3035 NO REMARKABLE OBSERVATIONS

3036 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING NOT CONFIRMED

3037 NO REMARKABLE OBSERVATIONS

3038 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

EXTERNA L EXAM ALOPECIA - EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 418

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3039 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - 0.1 CM IN DIAMETER, TAN

EXTERNAL EXAM INCI SORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

3040 NO REMARKABLE OBSERVATIONS

3041 THYMUS DISCOLORED FOCI; MODERATE; RIGHT

RED, LESS THAN 0.1 CM IN DIAMETER,

TISSUE SAVED

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, LESS THEN 0.1 CM IN DIAMETER, TAN

3042 NO REMARKABLE OBSERVATIONS

3043 THYMUS DISCOLORED FOCI; SLIGHT; BILATERAL

RED, LESS THAN 0.1 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, WHITE, 0.1 CM IN DIAMETER

KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

3044 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER

EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; EXTREME

EXTERNAL FINDING CONFIRMED

E XTERNAL EXAM ALOPECIA GENERAL

EXTERNAL FINDING CONFIRMED

3045 NO REMARKABLE OBSERVATIONS

3046 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

FILLED WITH THIN RED FLUID

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Scienc es 00

- 4206 PAGE 419

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PAR ENTAL NECROPSY OBSERVATIONS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3047 NO REMARKABLE OBSERVATIONS

3048 LUNG S DISCOLORED FOCI; SLIGHT

ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN

3049 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

3050 THYMUS DISCOLORED FOCI; SLIGHT

BOTH LOBES, RED, LESS THEN 0.1 CM IN DIAMETER

TISSUE SAVED

EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT

EXTERNAL FINDING CONFIRMED

3051 THYMUS DISCOLORED FOCI; SLIGHT; BILATERAL

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT

EXTERNAL FINDING NOT CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN -2 009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 420

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4026 NO REMARKABLE OBSERVATIONS

4027 NO REMARKABLE OBSERVATIONS

4028 NO REMARKABLE OBSERVATIONS

4029 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

4030 NO REMARKABLE OBSERVATIONS

4031 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - LESS THAN O.1 CM IN DIAMETER, TAN

LEFT LOBE - DISCOLORED FOCUS - 0.2 CM IN DIAMETER, RED

KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

4032 NO REMARKABLE OBSERVATIONS

4033 NO REMARKABLE OBSERVATIONS

4034 NO REMARKABLE OBSERVATIONS

4035 THYMUS DISCOLORED FOCI; MODERATE; BILATERAL

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

4052 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBE S, TAN, 0.1 CM IN DIAMETER

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONF IRMED

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 421

APPENDIX W

G ASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4037 KIDNEY DILATED RENAL PELVIS; SLIGHT; LEFT

4038 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/S NOUT; MODERATE

EXTERNAL FINDING CONFIRMED

4039 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THEN O.1 CM IN DIAMETER

4040 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

4041 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

4042 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

4043 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT; SLIGHT

EXTERNAL FINDING CONFIRMED

4044 THYMUS DISCOLORED FOCI; SLIGHT; BILATERAL

RED, LESS THAN 0.1 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, LESS THAN 0.1 CM IN DIAMETER, TAN

KIDNEY DILATED R ENAL PELVIS; MODERATE; RIGHT

FILLED WITH THIN RED FLUID

4045 LUNGS DISCOLORED FOCI; SLIG HT

ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER

4046 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:25

Huntingdon Life Sciences 00

- 4206 PAGE 422

APPENDIX W

GASOL INE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4046 (CONTINUED)

GONADS UNDESCENDED TESTE(S); LEFT

4047 THYMUS DI SCOLORED FOCI; EXTREME; BILATERAL

RED, 0.1

- 0.2 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THEN 0.1 CM IN DIAMETER

EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT; SLIGHT

EXTERNAL FINDING CONFIRMED

4048 NO REMARKABLE OBSERVATIONS

4049 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES - O.1 CM IN DIAMETER, TAN

4050 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN

4051 THYMUS DISCOLORED FOCI; EXTREME; BILATERAL

0.2 CM IN DIAMETER, RED

TISSUE SAVED

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 423

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1526 NO REMARKABLE OBSERVATIONS

1527 DPP THYMUS DISCOLORED FOCI; MODERATE; BILATERAL

RED - 0.1 CM IN DIAMETER

TISSUE SAVED

ADRENALS DISCOLORED ADRENALS; EXTREME; BILATERAL

RED

UTERUS FETUS (ES) IN UTERUS

ONE FETUS IN LEFT HORN

EXTERNAL EXAM - WITHIN NORMAL LIMITS

1528 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

1529 PNMD NO REMARKABLE OBSERVATIONS

1530 NO REMARKABLE OBSERVATIONS

1531 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAM ETER

1532 NP SALEWSKI'S TEST NO FOCI PRESENT

1533 OVARY CYST(S); LEFT

1.5 CM IN DIAMETER, CLEAR

1534 NO REMARKABLE OBSERVATIONS

1535 NP LIVER ADHESION

MEDIAN LOBE TO DIAPHRAGM - 1.5 CM IN DIAMETE R

TISSUE SAVED

GONADS FLUID

- FILLED VAGINA

1.5 X 1.0 X 1.0 CM, THICK WHITE FLUID

TISSUE SAVED

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT DPP=DIED POST PARTUM PNMD=PREGNANT, NO MATING DATE23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 424

A PPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1535 (CONTINUED)

SALEWSKI'S TEST NO FOCI PRESENT

1536 LIVER ADHESION

BETWEEN RIGHT LOBES - EXTREME

LIVER MASS; RIGHT

1.5 X 0.7 CM, RED, FIRM

15 37 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

1538 NO REMARKABLE OBSERVATIONS

1539 NO REMARKABLE OBSERVATIONS

1540 NO REMARKABLE OBSERVATIONS

1541 NP SALEWSKI'S TEST NO FOCI PRESENT

1542 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

O.2 CM IN DIAMETER, RED

KIDNEY MASS; LEFT

4.O X 3.0 CM, TAN, FIRM

1543 NO REMARKABLE OBSERVATIONS

1544 NO REMARKABLE OBSERVATIONS

1545 NO REMARKABLE OBSERVATIONS

1546 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 425

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1547 NO REMARKABLE OBSERVATIONS

1548 NNMD SALEWSKI'S TEST NO FOCI PRESENT

1549 NO REMARKABLE OBSERVATIONS

1550 NNMD SALEWSKI'S TEST NO FOCI PRESENT

1551 NO REMARKABLE OBSERVATIONS

-------------------------------------------------------------------------------------------------------------------------- ---------

NNMD=NOT PREG., NO MATING DATE23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 426

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2526 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

ABDOMINAL CAVITY MASS; MODERATE; RIGHT

RED, FIRM, 0.8 CM X 1.5 CM

ADHESION TO RENAL LYMPH NODE, RIGHT KIDNEY, RIGHT ADRENAL, AND

ACCESSORY LOBE OF LIVER

TISSUE SAVED

ADRENALS DISCOLORED ADRENALS; MODERATE; RIGHT

RED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

2527 NNMD SALEWSKI'S TEST NO FOCI PRESENT

2528 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN

2529 NO REMARKABLE OBSERVATIONS

2530 NO REMARKABLE OBSERVATIONS

2531 NO REMARKABLE OBSERVATIONS

2532 NP EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT; SLIGHT

EXTERNAL FINDING CON FIRMED

SALEWSKI'S TEST NO FOCI PRESENT

2533 NO REMARKABLE OBSERVATIONS

2534 NO REMARKABLE OBSERVATIONS

2535 LUNGS DISCOLORED FOCI; SLIGH T

ALL LOBES, WHITE, LESS THAN 0.1 CM IN DIAMETER,

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 427

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2536 NO REMARKABLE OBSERVATIONS

2537 LUNGS DISCOLORED FOC I; SLIGHT

ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN

2538 NNMD SALEWSKI'S TEST NO FOCI PRESENT

2539 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

2540 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

2541 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

2542 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

2543 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES - 0.2 CM IN DIAMETER, RED

2544 NO REMARKABLE OBSERVATIONS

2545 NO REMARKABLE OBSERVATIONS

2546 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 428

APP ENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2547 NO REMARKABLE OBSERVATIONS

2548 NO REMARKABLE OBSERVATIONS

2549 TLL NO REMARKABLE OBSERVATIONS

2550 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

TAN, LESS THAN 0.1 CM IN DIAMETER

2551 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------

TLL=TOTAL LITTER LOSS23

- JUN

- 2009 20:26

Hunt ingdon Life Sciences 00

- 4206 PAGE 429

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3526 NP SALEWSKI'S TEST NO FOCI PRESENT

3527 NO REMARKABLE OBSERVATIONS

3528 NO REMARKABLE OBSERVATIONS

35 29 EXTERNAL EXAM EAR(S) SWOLLEN/RED

EXTERNAL FINDING CONFIRMED

TISSUE SAVED

3530 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

3531 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

3532 NO REMARKABLE OBSERVATIONS

3533 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

3534 NO REMARKABLE OBSERVATIONS

3535 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES - TAN, 0.1 CM IN DIAMETER

RED, SLIGHT, 0.1 CM IN DIAMETER

3536 TLL EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT; SLIGHT

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

-------------------------------------------------------------------------------------------------------------------- ---------------

NP=NOT PREGNANT TLL=TOTAL LITTER LOSS23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 430

APPENDIX W

GASOLINE TBA VAPOR CONDENS ATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3537 NO REMARKABLE OBSERVATIONS

3538 NNMD SALEWSKI'S TEST NO FOCI PRESENT

3539 NNMD LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - LESS THAN 0.1 CM IN DIAMET ER, TAN

SALEWSKI'S TEST NO FOCI PRESENT

3540 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

3541 NO REMARKABLE OBSERVATIONS

3542 NO REMARKABLE OBSERVATIONS

3543 NO REMARKABLE OBSERVATIONS

3544 NO REMARKABLE OBSERVATIONS

3545 NO REMARKABLE OBSERVATIONS

3546 NO REMARKABLE OBSERVATIONS

3547 NO REMARKABLE OBSERVATIONS

3548 TLL NO REMARKABLE OBSERVATIONS

3549 NO REMARKABLE OBSERVATIONS

3550 NNMD SALEWSKI'S TEST NO FOCI PRESENT

3551 ADRENALS DISCOLORED FOC I; SLIGHT; LEFT

0.1 CM IN DIAMETER, RED

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 431

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4526 LUNGS DISCOLORED FOCI; SLIGHT

ALL L OBES - TAN, 0.1 CM IN DIAMETER

4527 NO REMARKABLE OBSERVATIONS

4528 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, TAN, 0.1 CM IN DIAMETER

4529 NO REMARKABLE OBSERVATIONS

4530 NP SALEWSKI'S TEST NO FOCI PRESENT

4531 LUNGS DISCOLORED FOCI; S LIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

4532 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

4533 NO REMARKABLE OBSERVATIONS

4534 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, TAN, 0.1 CM IN DIAMETE R

4535 NO REMARKABLE OBSERVATIONS

4536 NO REMARKABLE OBSERVATIONS

4537 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

4538 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 432

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4539 NP SALEWSKI'S TEST NO FOCI PRESENT

4540 NO REMARKABLE OBSERVATIONS

4541 NNMD LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN

SALEWSKI'S TEST NO FOCI PRESENT

4542 NO REMARKABLE OBSERVATIONS

4543 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

4544 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, WHITE, LESS THAN 0.1 CM IN DIAMETER

4545 NO REMARKABLE OBSERVATIONS

45 46 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

4547 PNMD LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN AND RED, 0.1 CM IN DIAMETER

4548 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

LEFT

- SLIGHT

LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, 0.1 CM IN DIAMETER, TAN

4549 DIED THYMUS DISCOLORE D FOCI; MODERATE; BILATERAL

RED, 0.2 CM IN DIAMETER

TISSUE SAVED

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT P REG., NO MATING DATE23

- JUN

- 2009 20:26

Huntingdon Life Sciences 00

- 4206 PAGE 433

APPENDIX W

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUC TION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4549 (CONTINUED)

LUNGS DISCOLORED FOCI; EXTREME

ALL LOBES, RED, 0.2 CM IN DIAMETER

4550 NO REMARKABLE OBSERVATIONS

4551 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, LESS THAN 0.1 CM IN DIAMETER

-----------------------------------------------------------------------------------------------------------------------------------23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 434

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1526 1F S STOMACH NO MILK IN STOMACH

LUNGS LUNG FLOATATION TEST - STILLBORN

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

1527 DPP 1F D a 0 LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH MILK IN STOMACH

2F D a 0 LUNGS LUNG FLOATA TION TEST - STILLBORN

STOMACH MILK IN STOMACH

3M U 1 NO REMARKABLE OBSERVATIONS

4M U 1 NO REMARKABLE OBSERVATIONS

5M U 1 NO REMARKABLE OBSERVATIONS

6F U 1 NO REMARKABLE OBSERVATIONS

7F U 1 NO REMARKABLE OBSERVATIONS

8F U 1 NO REMARKABLE OBSERVATIONS

9F U 1 NO REMARKABLE OBSERVATIONS

10F U 1 NO REMARKABLE OBSERVATIONS

11 F U 1 NO REMARKABLE OBSERVATIONS

12F U 1 NO REMARKABLE OBSERVATIONS

13F U 1 NO REMARKABLE OBSERVATIONS

14F U 1 NO REMARKABLE OBSERVATIONS

1528 1M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE U

- UNSCHEDULED SACRIFICE

DPP=DIED POST PARTUM, LITTER EXCLUDED

a Lung floatation test result superseded by observation of milk in stomach.23

- JUN

- 2009 20:28

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- 4206 PAGE 435

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1528 (CONTINUED)

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVA TIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO RE MARKABLE OBSERVATIONS

1529 PNMD 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

1530 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

6M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); SLIGHT; RIGHT

TISSUE SAVED

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

1531 1M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

---------------------------------------------------------------------------------------------------------------------- -------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE

PNMD=PREGNANT, NO MATING DATE23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 436

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INH ALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1531 (CONTINUED)

6M K 2 8 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

1533 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

1534 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMA RKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 437

APPENDIX X

GASOLINE TBA VAPOR CONDENSA TE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

------------------------------------------------------------------------------------------------------------------ -----------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1536 1M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSER VATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

1537 1M C 4 NO REMARKA BLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

1538 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUE SAVED

6F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 438

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTIO N TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1539 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKA BLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

1540 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

1542 1M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K -S CHEDULED SACRIFICE23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 439

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DA Y ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1543 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

1544 1M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE O BSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F D 9 STOMACH MILK IN STOMACH

1545 2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

4M C 4 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKAB LE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Un determined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 440

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

---------------------------------------------------------------------------------------------------------------------- -------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-------------------------------------------------------------------------------------------------------------- ---------------------

1545 (CONTINUED)

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

1546 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABL E OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO R EMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

1547 4M C 4 NO REMARKABLE OBSERVATIONS

5 M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11M C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

1549 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCH EDULED SACRIFICE23

- JUN

- 2009 20:28

Huntingdon Life Sciences 00

- 4206 PAGE 441

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1549 (CONTINUED)

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

1551 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20: 29

Huntingdon Life Sciences 00

- 4206 PAGE 442

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2526 1M S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2F D 0 LUNGS LUNG FLOATATION TEST

- FOUND DEAD

STOMACH MILK IN STOMACH

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

2528 1M S GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

2M K 28 GONADS SMALL TESTE(S); MODERATE; BILATERAL

TISSUE SAVED

GONADS SMALL EPIDIDYMIS(IDES); MODERATE; BILATERAL

TISSUE SAVED

3M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

2529 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:2 9

Huntingdon Life Sciences 00

- 4206 PAGE 443

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2529 (CONTINUED)

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

2530 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSE RVATIONS

2531 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARK ABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M C 4 N O REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

2533 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

------------------------------------------------------------------------------------------------------------------ -----------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 444

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSER VATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2533 (CONTINUED)

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

14 F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

2534 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3F K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

2535 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

5M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

2536 1M S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F D 3 STOMACH MILK IN STOMACH

8F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARK ABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 445

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATIO N WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2536 (CONTINUED)

10F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

2537 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSER VATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKAB LE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

2539 1F D 0 LUNGS LU NG FLOATATION TEST - FOUND DEAD

STOMACH NO MILK IN STOMACH

3M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

1 0F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

2540 1F Z GROSS EXAM CANNIBALIZED

GROSS EXAM SEX UNDETERMINED

2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED K

- SCHED ULED SACRIFICE23

- JUN

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 446

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALAT ION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2541 1M K 28 NO REMARKAB LE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

2543 1F D 0 LUNGS LUNG FLOATATION TEST

- FOUND DEAD

STOMACH NO MILK IN STOMACH

2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

2544 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3F K 28 NO REMARKABLE OBSERVATIONS

4F K 28 NO REMARKABLE OBSERVATIONS

2545 1M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:29

Hun tingdon Life Sciences 00

- 4206 PAGE 447

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2545 (CONTINUED)

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

2546 1F S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

2547 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKA BLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

2548 2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:29

Huntingdon Life Sciences 00

- 4206 PAGE 448

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TO XICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2550 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

2551 1M K 28 NO RE MARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 2 8 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 449

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICI TY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3527 1M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); SLIGHT; RIGHT

TISSUE SAVED

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT

TISSUE SAVED

4M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

17F K 28 NO REMARKABLE OBSERV ATIONS

18F K 28 NO REMARKABLE OBSERVATIONS

3528 1M D 0 LUNGS LUNG FLOATA TION TEST - FOUND DEAD

STOMACH MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5F D 4 STOMACH NO MILK IN STOMACH

6F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

3529 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 450

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3529 (CONTINUED)

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F K 2 8 NO REMARKABLE OBSERVATIONS

3530 2M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

3531 1M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

3532 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REM ARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 451

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3532 (CONTINUED)

13F K 28 NO REMARKABLE OBSERVATIONS

3533 1F S GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); SLIGHT; RIGHT

TISSUE SAVED

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

3534 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBS ERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARK ABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 N O REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F D 4 STOMACH MILK IN STOMACH

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

3535 2M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT

TISSUE SAVED

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 2 8 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 452

APPENDIX X

GASOLINE T BA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3535 (CONTINUED)

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMAR KABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

3536 TLL 1U Z GROSS EXAM CANNIBALIZED

GROSS EXAM SEX UNDETERMINED

2U Z GROSS EXAM CANNIBALIZED

GROSS EXAM SEX UNDETERMINED

3M S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

4M S LUNGS LUNG FLOATATION TEST - STILLBORN

STOMACH NO MILK IN STOMACH

5M S LUNGS LUNG FLOATATION TEST - STILLBORN

STOMACH NO MILK IN STOMACH

6F S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

7F D 0 LUNGS LUNG FLOATATION TEST

- FOUND DEAD

STOMACH NO MILK IN STOMACH

8F S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

9F S GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

10F D 0 LUNGS LUNG FLOATATION TEST

- FOUND DEAD

STOMACH NO MILK IN STOMACH

3537 2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED K

- SCHEDULED SACRIFICE

TLL=TOTAL LITTER LOSS23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 453

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STAT US DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3537 (CONTINUED)

8M K 28 NO REMARKABLE OBSERVATIONS

10M K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

3540 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIO NS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OB SERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMAR KABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

3541 1M S LUNGS LUNG FLOATATION TEST - STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

4M K 28 NO R EMARKABLE OBSERVATIONS

6M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARK ABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 N O REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 454

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3542 3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

10M K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

3543 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4F K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO RE MARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

3544 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

6F C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX COD ES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 455

APPENDIX X

GASOLI NE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3545 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M D 2 STOMACH MILK IN STOMACH

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

10M C 4 NO REM ARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

3546 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 2 8 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

3547 1M S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M Z 1 GROSS EXAM CANNIBALIZED

GROSS EXAM SEX UNDETERMINED

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT

TISSUE SAVED

6M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED Z

- CANNIBALIZED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:30

Huntingdon Life Science s 00

- 4206 PAGE 456

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3547 (CONTINUED)

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

3548 TLL 1M S LUNGS LUNG FLOATATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M Z 1 GROSS EXAM CANNIBALIZED

PARTIALLY

GROSS EXAM SEX UNDETERMINED

STOMACH MILK IN STOMACH UNDETERMINED

3M Z 1 GROSS EXAM CANNIBALIZED

PARTIALLY

GROSS EXAM SEX UNDETERMINED

STOMACH MILK IN STOMACH UNDETERMINED

9F Z 1 GROSS EXAM CANNIBALIZED

PARTIALLY

GROSS EXAM SEX UNDETERMINED

STOMACH MILK IN STOMACH UNDETERMINED

10F Z 1 GROSS EXAM CANNIBALIZED

PARTIALLY

GROSS EXAM SEX UNDETERMINED

STOMACH MILK IN STOMACH UNDETERMINED

12F Z 1 GROSS EXAM CANNIBALIZED

PARTIALLY

GROSS EXAM SEX UNDETERMINED

STOMACH MILK IN STOMACH UNDETERMINED

3549 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED Z

- CANNIBALIZED K

- SCHEDULED SACRIFICE

TLL=TOTAL LITTER LOSS23

- JUN

- 2009 20:30

Huntingdon Life Sciences 00

- 4206 PAGE 457

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INH ALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3549 (CONTINUED)

8F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

3551 1M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 458

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION RE PRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4526 1M C 4 NO REMARKABLE OBS ERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARK ABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 N O REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

4527 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F D 3 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

4528 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11M C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K- SCHEDULED SACRIFICE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 459

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORG AN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4529 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

4531 2M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

4532 1M D 1 LUNGS LUNG FLOATATION TEST

- FOUND DEAD

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8F D 1 GROSS EXAM AUTOLYSIS

POST

- MORT EM CHANGES OBVIOUS AT THE TIME OF NECROPSY

GROSS EXAM CANNIBALIZED

9F K 28 NO REMARKABLE OBSERVATIONS

11F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD

STOMACH NO MILK IN STOMACH

12F K 28 NO REMARKABLE OBSERVATIONS

13F D 1 LUNGS LUNG FLO ATATION TEST - FOUND DEAD

STOMACH NO MILK IN STOMACH

14F K 28 NO REMARKABLE OBSERVATIONS

15F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 460

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4532 (CONTINUED)

STOMACH NO MILK IN STOMACH

16F D 1 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIO US AT THE TIME OF NECROPSY

17F D 1 LUNGS LUNG FLOATATION TEST - FOUND DEAD

STOMACH NO MILK IN STOMACH

4533 1M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMA RKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

4534 1M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11M C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

17F C 4 NO REMARKABLE OBSERVATIONS

---------------------------------------------------------------------------------------------------------------- -------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 461

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBS ERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4535 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

4536 1M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT

TISSUE SAVED

2M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUE SAVED

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO R EMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F D 4 STOMACH NO MILK IN STOMACH

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

4537 1 M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

4538 1F D 0 LUNGS LUNG FLOATATION TEST

- FOUND DEAD

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SA CRIFICE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 462

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4538 (CONTINUED)

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11M C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

4540 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

4542 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE O BSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMA RKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

4543 2M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 463

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICIT Y STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4543 (CONTINUED)

12F K 28 NO REMARK ABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 N O REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

4544 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 KIDNEY DILATED RENAL PELVIS (PAPILLA REDUCED); MODERATE; RIGHT

TISSUE SAVED

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

4545 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

10M K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

4546 2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE23

- JUN

- 2009 20:31

H untingdon Life Sciences 00

- 4206 PAGE 464

APPENDI X X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4546 (CONTINUED)

9M K 28 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

4547 PNMD 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATION S

10F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

4548 1M K 28 NO REMAR KABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

4550 1M D 4 STOMACH NO MILK IN STOMACH

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE

PNMD=PREGNANT, NO MATING DATE23

- JUN

- 2009 20:31

Huntingdon Life Sciences 00

- 4206 PAGE 465

APPENDIX X

GASOLINE TBA VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALAT ION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4550 (CONTINUED)

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

4551 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE

# Huntingdon Life Sciences 00-4206 Page 466

211-TBA-1G Final Report

Individual Parental

Organ and Final Body Weights and Ratios

Preface Appendix Y

Key to Abbreviations:

g = Grams

SEM. VES. & CO. GL. = Seminal Vesicles and Coagulating Gland

Corresponding exposure levels for each group were as follows:

Group 1 - 0 mg/m 3



# Group 2 - 2000 mg/m 3



# Group 3 - 10000 mg/m 3



# Group 4 - 20000 mg/m 323-JUN-2009 20:39Huntingdon Life Sciences 00-4206 PAGE 467APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------TESTIS - RIGHT TESTIS - LEFT TESTES EPIDIDYMIS RIGHTAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------1026 542 1.6775 0.0031 0.8559 1.7092 0.0032 0.8720 3.3663 0.0062 1.7175 0.6914 0.0013 0.35281027 525 1.7957 0.0034 0.8581 1.8504 0.0035 0.8842 3.6221 0.0069 1.7308 0.7072 0.0013 0.33791028 542 1.6472 0.0030 0.8071 1.6779 0.0031 0.8222 3.3027 0.0061 1.6183 0.6588 0.0012 0.32281029 564 1.8337 0.0033 0.7928 1.8113 0.0032 0.7831 3.6387 0.0065 1.5732 0.6703 0.0012 0.28981030 614 1.7799 0.0029 0.7614 1.7344 0.0028 0.7419 3.4934 0.0057 1.4943 0.8438 0.0014 0.36091031 528 1.8174 0.0034 0.8606 1.8857 0.0036 0.8929 3.6881 0.0070 1.7464 0.7412 0.0014 0.35101032 518 1.5362 0.0030 0.7109 1.5810 0.0031 0.7317 3.1007 0.0060 1.4350 0.6475 0.0013 0.29971033 751 2.0144 0.0027 0.8732 2.1401 0.0028 0.9277 4.1334 0.0055 1.7918 0.8605 0.0011 0.37301034 610 1.8013 0.0030 0.8520 1.7688 0.0029 0.8367 3.5437 0.0058 1.6762 0.7314 0.0012 0.34601035 568 1.8811 0.0033 0.9111 1.8295 0.0032 0.8861 3.6990 0.0065 1.7915 0.7648 0.0013 0.37041036 547 1.9700 0.0036 0.9035 1.9397 0.0035 0.8896 3.8845 0.0071 1.7816 0.6719 0.0012 0.30821037 576 1.9895 0.0035 0.9305 2.0247 0.0035 0.9469 3.9868 0.0069 1.8646 0.7785 0.0014 0.36411038 598 1.8070 0.0030 0.7960 1.8445 0.0031 0.8125 3.6340 0.0061 1.6008 0.7194 0.0012 0.31691039 548 1.5714 0.0029 0.7671 1.5326 0.0028 0.7481 3.0998 0.0057 1.5131 0.6665 0.0012 0.32531040 665 1.8513 0.0028 0.8780 1.7928 0.0027 0.8502 3.6206 0.0054 1.7171 0.7602 0.0011 0.36051041 564 1.5634 0.0028 0.7665 1.5697 0.0028 0.7696 3.1172 0.0055 1.5283 0.7182 0.0013 0.35211042 595 1.7805 0.0030 0.8321 0.9571 0.0016 0.4473 2.7309 0.0046 1.2763 0.7795 0.0013 0.36431043 582 1.6597 0.0029 0.7601 1.6597 0.0029 0.7601 3.3021 0.0057 1.5124 0.7456 0.0013 0.34151044 550 1.8219 0.0033 0.8653 1.8634 0.0034 0.8850 3.6689 0.0067 1.7424 0.6967 0.0013 0.33091045 451 1.6719 0.0037 0.8174 1.7057 0.0038 0.8339 3.3692 0.0075 1.6472 0.7198 0.0016 0.35191046 545 1.9943 0.0037 0.9200 1.9373 0.0036 0.8938 3.9129 0.0072 1.8052 0.8787 0.0016 0.40541047 603 1.8357 0.0030 0.8714 1.8692 0.0031 0.8873 3.6885 0.0061 1.7508 0.7541 0.0012 0.35801048 487 1.6826 0.0035 0.8289 1.6291 0.0033 0.8025 3.3025 0.0068 1.6268 0.6643 0.0014 0.32721049 556 1.7775 0.0032 0.8282 1.7990 0.0032 0.8382 3.5629 0.0064 1.6601 0.8219 0.0015 0.38301050 560 1.9336 0.0035 0.8822 1.8884 0.0034 0.8616 3.8223 0.0068 1.7439 0.7487 0.0013 0.34161051 613 1.9099 0.0031 0.8390 1.8219 0.0030 0.8004 3.7071 0.0060 1.6286 0.7492 0.0012 0.3291-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:40Huntingdon Life Sciences 00-4206 PAGE 468APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------TESTIS - RIGHT TESTIS - LEFT TESTES EPIDIDYMIS RIGHTAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------2026 607 1.6941 0.0028 0.7441 1.6565 0.0027 0.7276 3.3340 0.0055 1.4644 0.7434 0.0012 0.32652027 585 1.6057 0.0027 0.7936 1.6643 0.0028 0.8226 3.2515 0.0056 1.6070 0.7584 0.0013 0.37482028 589 1.7704 0.0030 0.8151 1.7615 0.0030 0.8110 3.5215 0.0060 1.6213 0.7744 0.0013 0.35652029 645 1.7032 0.0026 0.7238 1.7232 0.0027 0.7323 3.4130 0.0053 1.4504 0.8355 0.0013 0.35502030 613 1.8846 0.0031 0.8379 1.8015 0.0029 0.8009 3.6716 0.0060 1.6323 0.7782 0.0013 0.34602031 552 1.7984 0.0033 0.8024 1.7839 0.0032 0.7960 3.5641 0.0065 1.5903 0.8168 0.0015 0.36442032 598 1.9493 0.0033 0.8477 1.8694 0.0031 0.8129 3.8133 0.0064 1.6582 0.7606 0.0013 0.33082033 671 1.8059 0.0027 0.9097 1.8474 0.0028 0.9306 3.6321 0.0054 1.8296 0.6794 0.0010 0.34222034 573 1.6133 0.0028 0.7636 1.5910 0.0028 0.7530 3.1927 0.0056 1.5111 0.6915 0.0012 0.32732035 551 1.6294 0.0030 0.7891 1.6923 0.0031 0.8196 3.3063 0.0060 1.6013 0.7380 0.0013 0.35742036 605 1.7887 0.0030 0.8190 1.8153 0.0030 0.8312 3.5825 0.0059 1.6404 0.7516 0.0012 0.34422037 558 1.7860 0.0032 0.8413 1.7683 0.0032 0.8330 3.5350 0.0063 1.6653 0.7731 0.0014 0.36422038 538 1.5582 0.0029 0.7542 1.5816 0.0029 0.7656 3.1261 0.0058 1.5132 0.5949 0.0011 0.28802039 604 1.9730 0.0033 0.9120 2.0268 0.0034 0.9369 3.9862 0.0066 1.8426 0.7854 0.0013 0.36312040 531 1.7556 0.0033 0.8167 1.6959 0.0032 0.7889 3.4346 0.0065 1.5977 0.7572 0.0014 0.35222041 575 2.0638 0.0036 0.9320 2.0127 0.0035 0.9090 4.0674 0.0071 1.8369 0.7748 0.0013 0.34992042 653 1.9203 0.0029 0.8402 1.9439 0.0030 0.8505 3.8527 0.0059 1.6857 0.8588 0.0013 0.37582043 553 2.1654 0.0039 0.9826 2.1116 0.0038 0.9582 4.2677 0.0077 1.9366 0.7642 0.0014 0.34682044 493 1.5646 0.0032 0.7034 1.6059 0.0033 0.7219 3.1588 0.0064 1.4201 0.6267 0.0013 0.28172045 542 1.7487 0.0032 0.8008 1.7634 0.0033 0.8075 3.5011 0.0065 1.6032 0.8040 0.0015 0.36822046 596 2.0407 0.0034 0.8824 2.0318 0.0034 0.8785 4.0674 0.0068 1.7587 0.8397 0.0014 0.36312047 569 1.7547 0.0031 0.7806 1.7229 0.0030 0.7664 3.4621 0.0061 1.5401 0.7599 0.0013 0.33802048 522 1.6505 0.0032 0.7717 1.6470 0.0032 0.7701 3.2903 0.0063 1.5384 0.7256 0.0014 0.33932049 539 1.7000 0.0032 0.7884 1.7402 0.0032 0.8070 3.4343 0.0064 1.5926 0.7748 0.0014 0.35932050 553 1.8422 0.0033 0.9142 1.7230 0.0031 0.8551 3.5599 0.0064 1.7667 0.7050 0.0013 0.34992051 556 1.7750 0.0032 0.8350 1.7391 0.0031 0.8181 3.5223 0.0063 1.6570 0.8083 0.0015 0.3803-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 469APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------TESTIS - RIGHT TESTIS - LEFT TESTES EPIDIDYMIS RIGHTAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------3026 470 1.8077 0.0038 0.8812 1.8807 0.0040 0.9167 3.6776 0.0078 1.7926 0.7150 0.0015 0.34853027 560 1.9940 0.0036 0.9256 1.9662 0.0035 0.9127 3.9495 0.0070 1.8334 0.7203 0.0013 0.33443028 629 1.6855 0.0027 0.8002 1.7225 0.0027 0.8178 3.4042 0.0054 1.6162 0.7593 0.0012 0.36053029 578 1.7467 0.0030 0.8147 1.8088 0.0031 0.8437 3.5498 0.0061 1.6558 0.7152 0.0012 0.33363030 612 1.7683 0.0029 0.8157 1.7762 0.0029 0.8194 3.5260 0.0058 1.6266 0.7672 0.0013 0.35393031 509 1.7894 0.0035 0.8028 1.7429 0.0034 0.7819 3.5276 0.0069 1.5826 0.7850 0.0015 0.35223032 492 1.6839 0.0034 0.8095 1.7322 0.0035 0.8327 3.3993 0.0069 1.6340 0.6425 0.0013 0.30883033 503 1.8313 0.0036 0.8348 1.8700 0.0037 0.8524 3.6859 0.0073 1.6802 0.7452 0.0015 0.33973034 583 1.6200 0.0028 0.8241 1.6104 0.0028 0.8192 3.2177 0.0055 1.6368 0.6900 0.0012 0.35103035 480 1.8133 0.0038 0.8991 1.7706 0.0037 0.8780 3.5694 0.0074 1.7699 0.7561 0.0016 0.37493036 563 1.8143 0.0032 0.8376 1.8395 0.0033 0.8492 3.6414 0.0065 1.6811 0.7604 0.0014 0.35103037 464 2.0015 0.0043 0.8948 1.9580 0.0042 0.8754 3.9469 0.0085 1.7646 0.7031 0.0015 0.31433038 576 1.7407 0.0030 0.8374 1.6858 0.0029 0.8110 3.4080 0.0059 1.6396 0.7400 0.0013 0.35603039 515 1.6915 0.0033 0.7876 1.6885 0.0033 0.7862 3.3639 0.0065 1.5662 0.7287 0.0014 0.33933040 584 1.9116 0.0033 0.8539 1.9279 0.0033 0.8611 3.8225 0.0066 1.7074 0.8188 0.0014 0.36573041 633 1.9975 0.0032 0.8795 1.9809 0.0031 0.8722 3.9663 0.0063 1.7463 0.9415 0.0015 0.41453042 523 1.8043 0.0035 0.8897 1.8187 0.0035 0.8968 3.6080 0.0069 1.7792 0.6817 0.0013 0.33623043 495 1.8241 0.0037 0.8701 1.7641 0.0036 0.8415 3.5758 0.0072 1.7057 0.9058 0.0018 0.43213044 595 1.6881 0.0028 0.7606 1.6719 0.0028 0.7533 3.3453 0.0056 1.5072 0.7515 0.0013 0.33863045 546 1.8612 0.0034 0.8313 1.8379 0.0034 0.8209 3.6862 0.0068 1.6464 0.7477 0.0014 0.33393046 637 1.9213 0.0030 0.8503 1.8485 0.0029 0.8181 3.7580 0.0059 1.6632 0.7582 0.0012 0.33563047 553 1.6293 0.0029 0.7630 1.6722 0.0030 0.7831 3.2902 0.0060 1.5408 0.6906 0.0012 0.32343048 603 1.6465 0.0027 0.7652 1.6631 0.0028 0.7730 3.3032 0.0055 1.5352 0.6651 0.0011 0.30913049 558 1.6468 0.0030 0.7863 1.6451 0.0029 0.7855 3.3144 0.0059 1.5826 0.6898 0.0012 0.32943050 589 1.8873 0.0032 0.8342 1.8845 0.0032 0.8330 3.7407 0.0063 1.6535 0.9014 0.0015 0.39843051 608 1.7028 0.0028 0.7690 1.6659 0.0027 0.7524 3.3616 0.0055 1.5182 0.7328 0.0012 0.3310-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 470APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------TESTIS - RIGHT TESTIS - LEFT TESTES EPIDIDYMIS RIGHTAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------4026 554 1.7263 0.0031 0.7462 1.7537 0.0032 0.7580 3.4667 0.0063 1.4984 0.7823 0.0014 0.33814027 503 1.5599 0.0031 0.7258 1.5541 0.0031 0.7231 3.1031 0.0062 1.4438 0.6594 0.0013 0.30684028 565 1.7875 0.0032 0.8668 1.7637 0.0031 0.8553 3.5403 0.0063 1.7168 0.6965 0.0012 0.33784029 564 2.0014 0.0035 0.9830 1.9344 0.0034 0.9501 3.9209 0.0069 1.9257 0.7567 0.0013 0.37164030 557 1.6792 0.0030 0.7959 1.7768 0.0032 0.8422 3.4468 0.0062 1.6337 0.6889 0.0012 0.32654031 513 1.9754 0.0039 0.9414 1.8831 0.0037 0.8974 3.8533 0.0075 1.8363 0.6962 0.0014 0.33184032 558 1.8769 0.0034 0.8547 1.8801 0.0034 0.8561 3.7478 0.0067 1.7066 0.6775 0.0012 0.30854033 561 2.1379 0.0038 1.0467 2.1572 0.0038 1.0561 4.2852 0.0076 2.0979 0.7110 0.0013 0.34814034 492 1.8611 0.0038 0.9292 1.7740 0.0036 0.8857 3.6213 0.0074 1.8079 0.6824 0.0014 0.34074035 586 2.1499 0.0037 0.9477 2.1259 0.0036 0.9371 4.2575 0.0073 1.8768 0.7740 0.0013 0.34124052 522 1.7044 0.0033 0.8013 1.7873 0.0034 0.8403 3.4703 0.0066 1.6315 0.6915 0.0013 0.32514037 498 1.6462 0.0033 0.8055 1.7005 0.0034 0.8320 3.3314 0.0067 1.6300 0.6047 0.0012 0.29594038 551 1.7085 0.0031 0.7713 1.6888 0.0031 0.7624 3.3838 0.0061 1.5277 0.7560 0.0014 0.34134039 534 1.6160 0.0030 0.7498 1.5942 0.0030 0.7397 3.2021 0.0060 1.4857 0.7170 0.0013 0.33274040 503 2.0579 0.0041 0.9950 2.1043 0.0042 1.0175 4.1492 0.0082 2.0062 0.8116 0.0016 0.39244041 562 1.6441 0.0029 0.8022 1.6477 0.0029 0.8040 3.2787 0.0058 1.5998 0.6879 0.0012 0.33574042 492 1.7865 0.0036 0.8797 1.7886 0.0036 0.8807 3.5693 0.0073 1.7576 0.6431 0.0013 0.31674043 532 1.6007 0.0030 0.7668 1.5841 0.0030 0.7589 3.1792 0.0060 1.5230 0.6885 0.0013 0.32984044 586 1.8316 0.0031 0.8917 1.8256 0.0031 0.8888 3.6479 0.0062 1.7759 0.7026 0.0012 0.34204045 590 1.7733 0.0030 0.7805 1.8175 0.0031 0.8000 3.5824 0.0061 1.5768 0.7718 0.0013 0.33974046 511 1.6875 0.0033 0.7997 1.7226 0.0034 0.8164 3.3976 0.0067 1.6102 0.7120 0.0014 0.33744047 525 1.6193 0.0031 0.7698 1.5763 0.0030 0.7493 3.1847 0.0061 1.5139 0.7768 0.0015 0.36934048 515 1.6987 0.0033 0.7793 1.7239 0.0033 0.7909 3.4074 0.0066 1.5632 0.7269 0.0014 0.33354049 459 1.7213 0.0038 0.8235 1.6985 0.0037 0.8126 3.4138 0.0074 1.6333 0.6218 0.0014 0.29754050 515 1.9544 0.0038 0.9645 1.9880 0.0039 0.9811 3.9455 0.0077 1.9470 0.7201 0.0014 0.35544051 587 1.7597 0.0030 0.8378 1.8042 0.0031 0.8589 3.5684 0.0061 1.6988 0.6651 0.0011 0.3166-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:39Huntingdon Life Sciences 00-4206 PAGE 471APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM.VES & CO.GL.Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------1026 542 0.6660 0.0012 0.3398 1.3521 0.0025 0.6898 0.9057 0.0017 0.4621 2.6832 0.0049 1.36901027 525 0.7206 0.0014 0.3443 1.4207 0.0027 0.6789 0.8817 0.0017 0.4213 2.3377 0.0045 1.11711028 542 0.7365 0.0014 0.3609 1.3754 0.0025 0.6740 0.8784 0.0016 0.4304 2.2008 0.0041 1.07841029 564 0.6382 0.0011 0.2759 1.2987 0.0023 0.5615 1.0690 0.0019 0.4622 2.5715 0.0046 1.11181030 614 0.8631 0.0014 0.3692 1.6928 0.0028 0.7241 1.4388 0.0023 0.6155 1.7340 0.0028 0.74171031 528 0.8036 0.0015 0.3805 1.5308 0.0029 0.7249 0.8645 0.0016 0.4094 2.1202 0.0040 1.00401032 518 0.6476 0.0013 0.2997 1.2796 0.0025 0.5922 1.0039 0.0019 0.4646 2.0313 0.0039 0.94011033 751 0.8961 0.0012 0.3885 1.7390 0.0023 0.7539 1.1209 0.0015 0.4859 2.3333 0.0031 1.01151034 610 0.7235 0.0012 0.3422 1.4371 0.0024 0.6798 1.0890 0.0018 0.5151 1.9893 0.0033 0.94101035 568 0.7159 0.0013 0.3467 1.4696 0.0026 0.7118 1.1267 0.0020 0.5457 2.6228 0.0046 1.27031036 547 0.6905 0.0013 0.3167 1.3528 0.0025 0.6205 1.1173 0.0020 0.5125 1.7191 0.0031 0.78851037 576 0.7965 0.0014 0.3725 1.5591 0.0027 0.7292 1.1906 0.0021 0.5568 2.0920 0.0036 0.97841038 598 0.7499 0.0013 0.3303 1.4609 0.0024 0.6435 1.2779 0.0021 0.5629 2.8878 0.0048 1.27211039 548 0.6088 0.0011 0.2972 1.2710 0.0023 0.6204 1.1972 0.0022 0.5844 2.1402 0.0039 1.04471040 665 0.7573 0.0011 0.3591 1.5067 0.0023 0.7146 1.2602 0.0019 0.5976 2.5024 0.0038 1.18681041 564 0.6726 0.0012 0.3298 1.3880 0.0025 0.6805 0.9892 0.0018 0.4850 2.1511 0.0038 1.05461042 595 0.5621 0.0009 0.2627 1.3337 0.0022 0.6233 0.8234 0.0014 0.3848 1.9761 0.0033 0.92351043 582 0.7194 0.0012 0.3295 1.4581 0.0025 0.6678 1.2367 0.0021 0.5664 2.6118 0.0045 1.19621044 550 0.7206 0.0013 0.3422 1.4072 0.0026 0.6683 0.9603 0.0017 0.4561 1.9238 0.0035 0.91371045 451 0.7273 0.0016 0.3556 1.4380 0.0032 0.7030 0.9303 0.0021 0.4548 2.5091 0.0056 1.22671046 545 0.8515 0.0016 0.3928 1.7163 0.0032 0.7918 1.1426 0.0021 0.5271 2.3376 0.0043 1.07841047 603 0.7823 0.0013 0.3713 1.5349 0.0025 0.7286 1.0079 0.0017 0.4784 2.6165 0.0043 1.24201048 487 0.6223 0.0013 0.3066 1.2770 0.0026 0.6291 0.7636 0.0016 0.3762 1.9307 0.0040 0.95111049 556 0.7397 0.0013 0.3447 1.5521 0.0028 0.7232 1.1567 0.0021 0.5390 2.0049 0.0036 0.93421050 560 0.6960 0.0012 0.3175 1.4589 0.0026 0.6656 0.9894 0.0018 0.4514 1.9378 0.0035 0.88411051 613 0.7015 0.0011 0.3082 1.4420 0.0024 0.6335 0.7654 0.0012 0.3362 2.3169 0.0038 1.0178-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:40Huntingdon Life Sciences 00-4206 PAGE 472APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM.VES & CO.GL.Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------2026 607 0.7131 0.0012 0.3132 1.4480 0.0024 0.6360 1.1328 0.0019 0.4976 1.8866 0.0031 0.82872027 585 0.7245 0.0012 0.3581 1.4726 0.0025 0.7278 1.1009 0.0019 0.5441 1.6055 0.0027 0.79352028 589 0.8070 0.0014 0.3715 1.5725 0.0027 0.7240 1.0919 0.0019 0.5027 2.3523 0.0040 1.08302029 645 0.8439 0.0013 0.3586 1.6665 0.0026 0.7082 1.5187 0.0024 0.6454 2.2828 0.0035 0.97012030 613 0.7113 0.0012 0.3162 1.4737 0.0024 0.6552 1.0588 0.0017 0.4707 2.0901 0.0034 0.92922031 552 0.7482 0.0014 0.3338 1.5430 0.0028 0.6885 1.0254 0.0019 0.4575 2.3120 0.0042 1.03162032 598 0.7828 0.0013 0.3404 1.5411 0.0026 0.6702 1.4670 0.0025 0.6379 2.6780 0.0045 1.16462033 671 0.7161 0.0011 0.3607 1.3786 0.0021 0.6944 1.1150 0.0017 0.5617 2.1388 0.0032 1.07742034 573 0.7155 0.0012 0.3387 1.3940 0.0024 0.6598 1.1615 0.0020 0.5497 1.7326 0.0030 0.82002035 551 0.7674 0.0014 0.3717 1.4911 0.0027 0.7222 1.3706 0.0025 0.6638 2.2360 0.0041 1.08292036 605 0.7253 0.0012 0.3321 1.4619 0.0024 0.6694 1.3917 0.0023 0.6373 1.9760 0.0033 0.90482037 558 0.7478 0.0013 0.3523 1.5110 0.0027 0.7118 0.8153 0.0015 0.3841 2.2832 0.0041 1.07562038 538 0.5976 0.0011 0.2893 1.1797 0.0022 0.5710 0.8928 0.0017 0.4322 1.9856 0.0037 0.96112039 604 0.8388 0.0014 0.3877 1.6211 0.0027 0.7494 0.8486 0.0014 0.3923 1.8095 0.0030 0.83652040 531 0.7005 0.0013 0.3259 1.4516 0.0027 0.6753 1.0540 0.0020 0.4903 2.5760 0.0049 1.19832041 575 0.7423 0.0013 0.3352 1.5104 0.0026 0.6821 1.2041 0.0021 0.5438 2.1296 0.0037 0.96172042 653 0.9001 0.0014 0.3938 1.7532 0.0027 0.7671 0.9186 0.0014 0.4019 2.0465 0.0031 0.89542043 553 0.7304 0.0013 0.3314 1.4873 0.0027 0.6749 1.0866 0.0020 0.4931 1.8370 0.0033 0.83362044 493 0.6578 0.0013 0.2957 1.2797 0.0026 0.5753 1.1374 0.0023 0.5113 1.8954 0.0038 0.85212045 542 0.7590 0.0014 0.3476 1.5594 0.0029 0.7141 1.2199 0.0023 0.5586 1.9958 0.0037 0.91392046 596 0.8375 0.0014 0.3621 1.6791 0.0028 0.7260 1.1027 0.0018 0.4768 2.5145 0.0042 1.08732047 569 0.6987 0.0012 0.3108 1.4471 0.0025 0.6438 1.1655 0.0021 0.5185 2.2601 0.0040 1.00542048 522 0.6951 0.0013 0.3250 1.4157 0.0027 0.6619 1.0777 0.0021 0.5039 2.0656 0.0040 0.96582049 539 0.7987 0.0015 0.3704 1.5698 0.0029 0.7280 1.2332 0.0023 0.5719 2.2375 0.0042 1.03762050 553 0.7461 0.0013 0.3703 1.5279 0.0028 0.7583 1.1182 0.0020 0.5549 1.8408 0.0033 0.91352051 556 0.8064 0.0015 0.3794 1.6042 0.0029 0.7547 0.7759 0.0014 0.3650 1.7254 0.0031 0.8117-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 473APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM.VES & CO.GL.Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------3026 470 0.7020 0.0015 0.3422 1.4032 0.0030 0.6840 0.9439 0.0020 0.4601 2.0941 0.0045 1.02083027 560 0.6993 0.0012 0.3246 1.4095 0.0025 0.6543 0.9840 0.0018 0.4568 2.4736 0.0044 1.14833028 629 0.7754 0.0012 0.3681 1.5200 0.0024 0.7216 1.2795 0.0020 0.6075 2.6763 0.0043 1.27063029 578 NT - - NT - - 1.0106 0.0017 0.4714 2.1564 0.0037 1.00583030 612 0.7565 0.0012 0.3490 1.4998 0.0025 0.6919 1.3336 0.0022 0.6152 2.9086 0.0048 1.34183031 509 0.7999 0.0016 0.3589 1.5799 0.0031 0.7088 1.0587 0.0021 0.4750 2.1199 0.0042 0.95113032 492 0.6503 0.0013 0.3126 1.2823 0.0026 0.6164 1.0527 0.0021 0.5060 1.6005 0.0033 0.76943033 503 0.6882 0.0014 0.3137 1.4175 0.0028 0.6462 0.8402 0.0017 0.3830 2.0951 0.0042 0.95513034 583 0.7170 0.0012 0.3647 1.4015 0.0024 0.7129 1.0953 0.0019 0.5572 2.8447 0.0049 1.44713035 480 0.6766 0.0014 0.3355 1.4152 0.0029 0.7017 1.1480 0.0024 0.5692 2.2294 0.0046 1.10553036 563 0.7437 0.0013 0.3433 1.4952 0.0027 0.6903 1.2016 0.0021 0.5547 2.6152 0.0046 1.20733037 464 0.6767 0.0015 0.3025 1.3642 0.0029 0.6099 0.9638 0.0021 0.4309 1.9637 0.0042 0.87793038 576 0.7201 0.0013 0.3464 1.4413 0.0025 0.6934 1.1506 0.0020 0.5535 2.3384 0.0041 1.12503039 515 0.6946 0.0013 0.3234 1.4115 0.0027 0.6572 0.9147 0.0018 0.4259 1.7086 0.0033 0.79553040 584 0.7304 0.0013 0.3262 1.5408 0.0026 0.6882 1.0910 0.0019 0.4873 2.1297 0.0036 0.95133041 633 0.8839 0.0014 0.3892 1.8126 0.0029 0.7981 1.0632 0.0017 0.4681 2.3970 0.0038 1.05543042 523 0.6610 0.0013 0.3260 1.3353 0.0026 0.6585 1.1815 0.0023 0.5826 2.4976 0.0048 1.23163043 495 0.8462 0.0017 0.4036 1.7450 0.0035 0.8324 1.2342 0.0025 0.5887 2.4680 0.0050 1.17733044 595 0.8436 0.0014 0.3801 1.5894 0.0027 0.7161 1.1518 0.0019 0.5189 2.7763 0.0047 1.25093045 546 0.6880 0.0013 0.3073 1.4264 0.0026 0.6371 1.1350 0.0021 0.5069 2.2056 0.0040 0.98513046 637 0.7203 0.0011 0.3188 1.4963 0.0024 0.6622 1.1458 0.0018 0.5071 2.7745 0.0044 1.22793047 553 0.6789 0.0012 0.3179 1.3636 0.0025 0.6386 1.0390 0.0019 0.4866 2.0352 0.0037 0.95313048 603 0.6771 0.0011 0.3147 1.3305 0.0022 0.6184 1.0670 0.0018 0.4959 2.5289 0.0042 1.17543049 558 0.7114 0.0013 0.3397 1.3959 0.0025 0.6665 1.1725 0.0021 0.5599 2.5642 0.0046 1.22443050 589 0.9502 0.0016 0.4200 1.8274 0.0031 0.8078 1.0546 0.0018 0.4662 2.5846 0.0044 1.14253051 608 0.7796 0.0013 0.3521 1.5075 0.0025 0.6808 1.2741 0.0021 0.5754 2.2146 0.0036 1.0002-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTNT=NOT TAKEN: left epididymis lost during necropsy; no weight for the left epididymis or total epididymis was taken.23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 474APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------EPIDIDYMIS LEFT EPIDIDYMIDES PROSTATE SEM.VES & CO.GL.Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------4026 554 0.7099 0.0013 0.3068 1.4820 0.0027 0.6406 1.0049 0.0018 0.4343 2.3174 0.0042 1.00164027 503 0.6080 0.0012 0.2829 1.2687 0.0025 0.5903 0.9801 0.0019 0.4560 2.0843 0.0041 0.96984028 565 0.6362 0.0011 0.3085 1.3242 0.0023 0.6422 1.1984 0.0021 0.5812 2.2365 0.0040 1.08464029 564 0.6739 0.0012 0.3310 1.4150 0.0025 0.6950 1.1026 0.0020 0.5415 2.2827 0.0040 1.12114030 557 0.7389 0.0013 0.3502 1.4187 0.0025 0.6724 1.1533 0.0021 0.5466 2.3080 0.0041 1.09394031 513 0.6876 0.0013 0.3277 1.3730 0.0027 0.6543 1.4275 0.0028 0.6803 2.4848 0.0048 1.18414032 558 0.6610 0.0012 0.3010 1.3301 0.0024 0.6057 1.2606 0.0023 0.5740 2.2693 0.0041 1.03344033 561 0.7674 0.0014 0.3757 1.4691 0.0026 0.7192 1.9336 0.0034 0.9466 2.2281 0.0040 1.09084034 492 0.6866 0.0014 0.3428 1.3558 0.0028 0.6769 1.1357 0.0023 0.5670 3.2960 0.0067 1.64554035 586 0.7470 0.0013 0.3293 1.5079 0.0026 0.6647 0.7536 0.0013 0.3322 1.7678 0.0030 0.77934052 522 0.6550 0.0013 0.3079 1.3367 0.0026 0.6284 1.0195 0.0020 0.4793 1.6185 0.0031 0.76094037 498 0.6072 0.0012 0.2971 1.2009 0.0024 0.5876 0.9774 0.0020 0.4782 2.3974 0.0048 1.17304038 551 0.7624 0.0014 0.3442 1.5048 0.0027 0.6794 1.2689 0.0023 0.5729 1.9664 0.0036 0.88784039 534 0.7093 0.0013 0.3291 1.4159 0.0027 0.6569 0.8636 0.0016 0.4007 1.9148 0.0036 0.88844040 503 0.7995 0.0016 0.3866 1.6030 0.0032 0.7751 0.9294 0.0018 0.4494 2.0037 0.0040 0.96884041 562 0.6360 0.0011 0.3103 1.3183 0.0023 0.6433 0.9908 0.0018 0.4835 1.8708 0.0033 0.91294042 492 0.6517 0.0013 0.3209 1.2895 0.0026 0.6350 1.2384 0.0025 0.6098 2.5260 0.0051 1.24384043 532 0.6515 0.0012 0.3121 1.3365 0.0025 0.6403 0.9862 0.0019 0.4725 2.2523 0.0042 1.07904044 586 0.6785 0.0012 0.3303 1.3712 0.0023 0.6675 1.2708 0.0022 0.6187 2.2590 0.0039 1.09984045 590 0.8010 0.0014 0.3526 1.5660 0.0027 0.6893 1.0441 0.0018 0.4596 2.0211 0.0034 0.88964046 511 0.7240 0.0014 0.3431 1.4275 0.0028 0.6765 1.3984 0.0027 0.6627 2.6634 0.0052 1.26224047 525 0.7471 0.0014 0.3552 1.5166 0.0029 0.7210 1.1587 0.0022 0.5508 1.7321 0.0033 0.82344048 515 0.7786 0.0015 0.3572 1.4957 0.0029 0.6862 1.1645 0.0023 0.5342 1.9966 0.0039 0.91604049 459 0.6674 0.0015 0.3193 1.2819 0.0028 0.6133 1.1250 0.0025 0.5383 2.1268 0.0046 1.01764050 515 0.6757 0.0013 0.3334 1.4037 0.0027 0.6927 1.0605 0.0021 0.5233 1.7297 0.0034 0.85364051 587 0.6876 0.0012 0.3274 1.3430 0.0023 0.6394 0.9352 0.0016 0.4452 1.7378 0.0030 0.8273-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:39Huntingdon Life Sciences 00-4206 PAGE 475APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------1026 542 0.0525 0.0001 0.0268 1.9600 0.0036 1.0000 4.0573 0.0075 2.0701 22.1260 0.0408 11.28881027 525 0.0568 0.0001 0.0271 2.0927 0.0040 1.0000 4.1085 0.0078 1.9633 21.3042 0.0406 10.18021028 542 0.0721 0.0001 0.0353 2.0408 0.0038 1.0000 3.7434 0.0069 1.8343 17.9095 0.0330 8.77571029 564 0.0739 0.0001 0.0320 2.3129 0.0041 1.0000 3.9539 0.0070 1.7095 20.1313 0.0357 8.70391030 614 0.0814 0.0001 0.0348 2.3378 0.0038 1.0000 4.7951 0.0078 2.0511 24.1073 0.0393 10.31201031 528 0.0593 0.0001 0.0281 2.1118 0.0040 1.0000 3.7363 0.0071 1.7692 18.1974 0.0345 8.61701032 518 0.0764 0.0001 0.0354 2.1608 0.0042 1.0000 4.0704 0.0079 1.8837 20.1219 0.0389 9.31221033 751 0.0652 0.0001 0.0283 2.3068 0.0031 1.0000 5.6246 0.0075 2.4383 24.7549 0.0330 10.73131034 610 0.0786 0.0001 0.0372 2.1141 0.0035 1.0000 4.1620 0.0068 1.9687 19.4239 0.0319 9.18781035 568 0.0737 0.0001 0.0357 2.0647 0.0036 1.0000 3.7682 0.0066 1.8251 19.0380 0.0335 9.22071036 547 0.0594 0.0001 0.0272 2.1803 0.0040 1.0000 4.3142 0.0079 1.9787 18.6908 0.0342 8.57261037 576 0.0762 0.0001 0.0356 2.1382 0.0037 1.0000 4.9742 0.0086 2.3263 21.3333 0.0370 9.97721038 598 0.0698 0.0001 0.0307 2.2701 0.0038 1.0000 4.2047 0.0070 1.8522 17.9105 0.0300 7.88971039 548 0.0627 0.0001 0.0306 2.0486 0.0037 1.0000 3.7899 0.0069 1.8500 18.4642 0.0337 9.01311040 665 0.0569 0.0001 0.0270 2.1086 0.0032 1.0000 4.4344 0.0067 2.1030 24.1149 0.0363 11.43651041 564 0.0640 0.0001 0.0314 2.0397 0.0036 1.0000 3.7713 0.0067 1.8489 20.0194 0.0355 9.81491042 595 0.0639 0.0001 0.0299 2.1397 0.0036 1.0000 3.9015 0.0066 1.8234 20.3243 0.0342 9.49871043 582 0.0674 0.0001 0.0309 2.1834 0.0038 1.0000 4.1631 0.0072 1.9067 22.7770 0.0391 10.43191044 550 0.0620 0.0001 0.0294 2.1056 0.0038 1.0000 3.9725 0.0072 1.8866 19.7231 0.0359 9.36701045 451 0.0623 0.0001 0.0305 2.0454 0.0045 1.0000 3.5813 0.0079 1.7509 17.1439 0.0380 8.38171046 545 0.0652 0.0001 0.0301 2.1676 0.0040 1.0000 4.2761 0.0078 1.9727 18.6120 0.0342 8.58651047 603 0.0588 0.0001 0.0279 2.1067 0.0035 1.0000 4.6003 0.0076 2.1837 21.8246 0.0362 10.35961048 487 0.0646 0.0001 0.0318 2.0300 0.0042 1.0000 3.5655 0.0073 1.7564 15.5860 0.0320 7.67781049 556 0.0718 0.0001 0.0335 2.1462 0.0039 1.0000 4.0360 0.0073 1.8805 19.7612 0.0355 9.20751050 560 0.0714 0.0001 0.0326 2.1918 0.0039 1.0000 4.0888 0.0073 1.8655 18.7089 0.0334 8.53591051 613 0.0574 0.0001 0.0252 2.2763 0.0037 1.0000 4.5208 0.0074 1.9860 20.9055 0.0341 9.1840-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:40Huntingdon Life Sciences 00-4206 PAGE 476APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------2026 607 0.0589 0.0001 0.0259 2.2767 0.0038 1.0000 4.5702 0.0075 2.0074 25.5318 0.0421 11.21442027 585 0.0713 0.0001 0.0352 2.0233 0.0035 1.0000 4.8185 0.0082 2.3815 21.5290 0.0368 10.64052028 589 0.0426 0.0001 0.0196 2.1720 0.0037 1.0000 4.5067 0.0076 2.0749 20.3840 0.0346 9.38492029 645 0.0704 0.0001 0.0299 2.3532 0.0037 1.0000 5.5555 0.0086 2.3608 26.7394 0.0415 11.36302030 613 0.0677 0.0001 0.0301 2.2493 0.0037 1.0000 4.7144 0.0077 2.0959 23.4842 0.0383 10.44072031 552 0.0785 0.0001 0.0350 2.2412 0.0041 1.0000 4.3966 0.0080 1.9617 20.0163 0.0363 8.93112032 598 0.0686 0.0001 0.0298 2.2996 0.0038 1.0000 4.1701 0.0070 1.8134 21.4018 0.0358 9.30672033 671 0.0594 0.0001 0.0299 1.9852 0.0030 1.0000 3.8794 0.0058 1.9542 22.4460 0.0335 11.30672034 573 0.0594 0.0001 0.0281 2.1128 0.0037 1.0000 3.7074 0.0065 1.7547 20.3094 0.0355 9.61262035 551 0.0808 0.0001 0.0391 2.0648 0.0037 1.0000 4.2406 0.0077 2.0538 20.5866 0.0374 9.97032036 605 0.0794 0.0001 0.0364 2.1839 0.0036 1.0000 4.4923 0.0074 2.0570 21.0306 0.0348 9.62982037 558 0.0569 0.0001 0.0268 2.1228 0.0038 1.0000 4.0845 0.0073 1.9241 18.8533 0.0338 8.88132038 538 0.0634 0.0001 0.0307 2.0659 0.0038 1.0000 4.0393 0.0075 1.9552 20.3720 0.0379 9.86112039 604 0.0703 0.0001 0.0325 2.1633 0.0036 1.0000 4.8134 0.0080 2.2250 23.0134 0.0381 10.63812040 531 0.0749 0.0001 0.0348 2.1497 0.0041 1.0000 4.2977 0.0081 1.9992 21.3710 0.0403 9.94142041 575 0.0774 0.0001 0.0350 2.2143 0.0039 1.0000 4.6990 0.0082 2.1221 20.4692 0.0356 9.24412042 653 0.0683 0.0001 0.0299 2.2855 0.0035 1.0000 5.1026 0.0078 2.2326 25.3389 0.0388 11.08682043 553 0.0714 0.0001 0.0324 2.2037 0.0040 1.0000 4.1064 0.0074 1.8634 21.2498 0.0385 9.64282044 493 0.0587 0.0001 0.0264 2.2244 0.0045 1.0000 3.9720 0.0081 1.7857 19.6869 0.0399 8.85042045 542 0.0634 0.0001 0.0290 2.1838 0.0040 1.0000 4.2644 0.0079 1.9527 18.2469 0.0337 8.35562046 596 0.0931 0.0002 0.0403 2.3127 0.0039 1.0000 4.4168 0.0074 1.9098 21.9979 0.0369 9.51182047 569 0.0569 0.0001 0.0253 2.2479 0.0040 1.0000 4.0449 0.0071 1.7994 16.6868 0.0294 7.42332048 522 0.0605 0.0001 0.0283 2.1388 0.0041 1.0000 3.7223 0.0071 1.7404 17.0217 0.0326 7.95852049 539 0.0618 0.0001 0.0287 2.1564 0.0040 1.0000 4.0904 0.0076 1.8969 19.3021 0.0358 8.95112050 553 0.0454 0.0001 0.0225 2.0150 0.0036 1.0000 3.9172 0.0071 1.9440 17.7877 0.0322 8.82762051 556 0.0463 0.0001 0.0218 2.1257 0.0038 1.0000 5.2743 0.0095 2.4812 18.7173 0.0337 8.8052-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 477APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------3026 470 0.0651 0.0001 0.0317 2.0515 0.0044 1.0000 4.3501 0.0092 2.1204 19.4364 0.0413 9.47423027 560 0.0676 0.0001 0.0314 2.1542 0.0038 1.0000 5.5738 0.0099 2.5874 21.2057 0.0378 9.84393028 629 0.0665 0.0001 0.0316 2.1063 0.0034 1.0000 5.4729 0.0087 2.5983 25.0903 0.0399 11.91203029 578 0.0646 0.0001 0.0301 2.1439 0.0037 1.0000 4.7685 0.0083 2.2242 22.0735 0.0382 10.29603030 612 0.0689 0.0001 0.0318 2.1677 0.0035 1.0000 4.9991 0.0082 2.3062 23.3981 0.0382 10.79403031 509 0.0762 0.0001 0.0342 2.2290 0.0044 1.0000 4.6741 0.0092 2.0969 18.7364 0.0368 8.40573032 492 0.0643 0.0001 0.0309 2.0803 0.0042 1.0000 4.1657 0.0085 2.0025 17.3445 0.0353 8.33753033 503 0.0675 0.0001 0.0308 2.1937 0.0044 1.0000 4.4314 0.0088 2.0201 20.4151 0.0406 9.30623034 583 0.0677 0.0001 0.0344 1.9658 0.0034 1.0000 5.8366 0.0100 2.9691 23.1594 0.0397 11.78123035 480 0.0523 0.0001 0.0259 2.0167 0.0042 1.0000 3.9855 0.0083 1.9762 17.7456 0.0369 8.79933036 563 0.0767 0.0001 0.0354 2.1661 0.0038 1.0000 4.7159 0.0084 2.1771 20.8790 0.0371 9.63903037 464 0.0612 0.0001 0.0274 2.2367 0.0048 1.0000 4.3945 0.0095 1.9647 17.4592 0.0376 7.80583038 576 0.0603 0.0001 0.0290 2.0786 0.0036 1.0000 4.9385 0.0086 2.3759 18.2503 0.0317 8.78013039 515 0.0619 0.0001 0.0288 2.1478 0.0042 1.0000 4.2287 0.0082 1.9689 17.0769 0.0332 7.95093040 584 0.0868 0.0001 0.0388 2.2388 0.0038 1.0000 5.2370 0.0090 2.3392 26.2080 0.0449 11.70633041 633 0.0548 0.0001 0.0241 2.2712 0.0036 1.0000 5.4608 0.0086 2.4044 25.0291 0.0396 11.02023042 523 0.0701 0.0001 0.0346 2.0279 0.0039 1.0000 4.3563 0.0083 2.1482 18.7716 0.0359 9.25673043 495 0.0730 0.0001 0.0348 2.0964 0.0042 1.0000 4.7973 0.0097 2.2884 17.8664 0.0361 8.52243044 595 0.0830 0.0001 0.0374 2.2195 0.0037 1.0000 4.7853 0.0080 2.1560 21.3215 0.0358 9.60643045 546 0.0878 0.0002 0.0392 2.2390 0.0041 1.0000 4.6883 0.0086 2.0939 20.5298 0.0376 9.16923046 637 0.1033 0.0002 0.0457 2.2595 0.0035 1.0000 5.1714 0.0081 2.2887 25.3733 0.0399 11.22963047 553 0.0633 0.0001 0.0296 2.1354 0.0039 1.0000 4.5468 0.0082 2.1292 19.4227 0.0352 9.09563048 603 0.0749 0.0001 0.0348 2.1516 0.0036 1.0000 4.5839 0.0076 2.1305 21.0167 0.0349 9.76793049 558 0.0582 0.0001 0.0278 2.0943 0.0038 1.0000 4.6459 0.0083 2.2184 18.9172 0.0339 9.03273050 589 0.0623 0.0001 0.0275 2.2623 0.0038 1.0000 5.0528 0.0086 2.2335 22.3741 0.0380 9.89003051 608 0.0586 0.0001 0.0265 2.2142 0.0036 1.0000 5.2383 0.0086 2.3658 21.7969 0.0358 9.8441-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 478APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------4026 554 0.0683 0.0001 0.0295 2.3136 0.0042 1.0000 4.4343 0.0080 1.9166 25.2198 0.0455 10.90074027 503 0.0863 0.0002 0.0402 2.1493 0.0043 1.0000 5.2125 0.0104 2.4252 28.2262 0.0561 13.13274028 565 0.0684 0.0001 0.0332 2.0621 0.0037 1.0000 5.8276 0.0103 2.8261 26.2643 0.0465 12.73674029 564 0.0856 0.0002 0.0420 2.0361 0.0036 1.0000 4.6920 0.0083 2.3044 20.3377 0.0360 9.98864030 557 0.0673 0.0001 0.0319 2.1098 0.0038 1.0000 4.7593 0.0085 2.2558 24.1424 0.0433 11.44304031 513 0.0780 0.0002 0.0372 2.0984 0.0041 1.0000 4.6146 0.0090 2.1991 19.9476 0.0389 9.50614032 558 0.0743 0.0001 0.0338 2.1960 0.0039 1.0000 5.0668 0.0091 2.3073 26.9067 0.0483 12.25264033 561 0.0702 0.0001 0.0344 2.0426 0.0036 1.0000 5.6209 0.0100 2.7518 21.0550 0.0375 10.30794034 492 0.0621 0.0001 0.0310 2.0030 0.0041 1.0000 4.6279 0.0094 2.3105 19.7124 0.0400 9.84144035 586 0.0646 0.0001 0.0285 2.2685 0.0039 1.0000 4.6238 0.0079 2.0383 19.7820 0.0338 8.72034052 522 0.0759 0.0001 0.0357 2.1270 0.0041 1.0000 4.4327 0.0085 2.0840 18.4764 0.0354 8.68664037 498 0.0524 0.0001 0.0256 2.0438 0.0041 1.0000 4.6940 0.0094 2.2967 20.4451 0.0411 10.00354038 551 0.0822 0.0001 0.0371 2.2150 0.0040 1.0000 5.5779 0.0101 2.5182 20.4384 0.0371 9.22734039 534 0.0906 0.0002 0.0420 2.1553 0.0040 1.0000 5.3236 0.0100 2.4700 23.1827 0.0434 10.75614040 503 0.0695 0.0001 0.0336 2.0682 0.0041 1.0000 5.0749 0.0101 2.4538 25.8386 0.0513 12.49334041 562 0.0754 0.0001 0.0368 2.0494 0.0036 1.0000 5.0627 0.0090 2.4703 20.7616 0.0369 10.13064042 492 0.0706 0.0001 0.0348 2.0308 0.0041 1.0000 4.0653 0.0083 2.0018 19.9851 0.0406 9.84104043 532 0.0629 0.0001 0.0301 2.0874 0.0039 1.0000 4.5341 0.0085 2.1721 23.7428 0.0447 11.37434044 586 0.0800 0.0001 0.0389 2.0541 0.0035 1.0000 5.5625 0.0095 2.7080 22.5998 0.0386 11.00234045 590 0.0641 0.0001 0.0282 2.2720 0.0038 1.0000 4.8709 0.0083 2.1439 22.7772 0.0386 10.02524046 511 0.0657 0.0001 0.0311 2.1101 0.0041 1.0000 4.9233 0.0096 2.3332 21.4057 0.0419 10.14444047 525 0.0911 0.0002 0.0433 2.1036 0.0040 1.0000 4.7923 0.0091 2.2781 21.4898 0.0409 10.21574048 515 0.0663 0.0001 0.0304 2.1798 0.0042 1.0000 4.7179 0.0092 2.1644 21.5532 0.0419 9.88774049 459 0.0752 0.0002 0.0360 2.0901 0.0046 1.0000 4.2238 0.0092 2.0209 17.4827 0.0381 8.36454050 515 0.0610 0.0001 0.0301 2.0264 0.0039 1.0000 4.9284 0.0096 2.4321 17.7900 0.0345 8.77914051 587 0.0542 0.0001 0.0258 2.1005 0.0036 1.0000 4.5607 0.0078 2.1712 20.4977 0.0349 9.7585-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:39Huntingdon Life Sciences 00-4206 PAGE 479APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------1026 542 1.9402 0.0036 0.9899 0.0099 0.0000 0.0051 0.9620 0.0018 0.49081027 525 1.6292 0.0031 0.7785 0.0132 0.0000 0.0063 0.8048 0.0015 0.38461028 542 1.7804 0.0033 0.8724 0.0135 0.0000 0.0066 0.6995 0.0013 0.34281029 564 2.0914 0.0037 0.9042 0.0119 0.0000 0.0051 0.9429 0.0017 0.40771030 614 2.3016 0.0038 0.9845 0.0154 0.0000 0.0066 1.1808 0.0019 0.50511031 528 1.6212 0.0031 0.7677 0.0110 0.0000 0.0052 0.7903 0.0015 0.37421032 518 1.8551 0.0036 0.8585 0.0137 0.0000 0.0063 0.9342 0.0018 0.43231033 751 2.4965 0.0033 1.0822 0.0166 0.0000 0.0072 1.0930 0.0015 0.47381034 610 2.1346 0.0035 1.0097 0.0126 0.0000 0.0060 0.7984 0.0013 0.37771035 568 2.1062 0.0037 1.0201 0.0113 0.0000 0.0055 0.7272 0.0013 0.35221036 547 2.2192 0.0041 1.0178 0.0134 0.0000 0.0061 0.8791 0.0016 0.40321037 576 2.0988 0.0036 0.9816 0.0121 0.0000 0.0057 0.9870 0.0017 0.46161038 598 2.0166 0.0034 0.8883 0.0127 0.0000 0.0056 1.1235 0.0019 0.49491039 548 1.7335 0.0032 0.8462 0.0100 0.0000 0.0049 0.6377 0.0012 0.31131040 665 2.0142 0.0030 0.9552 0.0135 0.0000 0.0064 1.1035 0.0017 0.52331041 564 1.8671 0.0033 0.9154 0.0113 0.0000 0.0055 0.9211 0.0016 0.45161042 595 1.9357 0.0033 0.9047 0.0147 0.0000 0.0069 0.9742 0.0016 0.45531043 582 1.9858 0.0034 0.9095 0.0115 0.0000 0.0053 0.8861 0.0015 0.40581044 550 2.1179 0.0039 1.0058 0.0136 0.0000 0.0065 0.8627 0.0016 0.40971045 451 1.6045 0.0036 0.7844 0.0101 0.0000 0.0049 0.6563 0.0015 0.32091046 545 2.1282 0.0039 0.9818 0.0132 0.0000 0.0061 0.8278 0.0015 0.38191047 603 1.8697 0.0031 0.8875 0.0140 0.0000 0.0066 0.9746 0.0016 0.46261048 487 1.6858 0.0035 0.8304 0.0107 0.0000 0.0053 0.7909 0.0016 0.38961049 556 2.0741 0.0037 0.9664 0.0123 0.0000 0.0057 0.9373 0.0017 0.43671050 560 1.9825 0.0035 0.9045 0.0111 0.0000 0.0051 0.7880 0.0014 0.35951051 613 1.9922 0.0032 0.8752 0.0142 0.0000 0.0062 1.0652 0.0017 0.4680-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:40Huntingdon Life Sciences 00-4206 PAGE 480APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------2026 607 2.0061 0.0033 0.8811 0.0117 0.0000 0.0051 0.8287 0.0014 0.36402027 585 1.9720 0.0034 0.9746 0.0140 0.0000 0.0069 0.7211 0.0012 0.35642028 589 1.8646 0.0032 0.8585 0.0139 0.0000 0.0064 1.0093 0.0017 0.46472029 645 2.5436 0.0039 1.0809 0.0098 0.0000 0.0042 1.0029 0.0016 0.42622030 613 2.1366 0.0035 0.9499 0.0130 0.0000 0.0058 0.9406 0.0015 0.41822031 552 2.7423 0.0050 1.2236 0.0136 0.0000 0.0061 0.9004 0.0016 0.40172032 598 1.9917 0.0033 0.8661 0.0142 0.0000 0.0062 0.6750 0.0011 0.29352033 671 2.0950 0.0031 1.0553 0.0127 0.0000 0.0064 0.8326 0.0012 0.41942034 573 1.8724 0.0033 0.8862 0.0136 0.0000 0.0064 0.6829 0.0012 0.32322035 551 1.8542 0.0034 0.8980 0.0133 0.0000 0.0064 0.8365 0.0015 0.40512036 605 2.0372 0.0034 0.9328 0.0134 0.0000 0.0061 1.0287 0.0017 0.47102037 558 1.8588 0.0033 0.8756 0.0119 0.0000 0.0056 0.9062 0.0016 0.42692038 538 1.7794 0.0033 0.8613 0.0124 0.0000 0.0060 0.7929 0.0015 0.38382039 604 2.1551 0.0036 0.9962 0.0120 0.0000 0.0055 0.7501 0.0012 0.34672040 531 2.0996 0.0040 0.9767 0.0149 0.0000 0.0069 0.8319 0.0016 0.38702041 575 1.9061 0.0033 0.8608 0.0161 0.0000 0.0073 0.9873 0.0017 0.44592042 653 1.9567 0.0030 0.8561 0.0145 0.0000 0.0063 0.9523 0.0015 0.41672043 553 1.9619 0.0036 0.8903 0.0119 0.0000 0.0054 1.0128 0.0018 0.45962044 493 2.0369 0.0041 0.9157 0.0085 0.0000 0.0038 0.7496 0.0015 0.33702045 542 1.9541 0.0036 0.8948 0.0112 0.0000 0.0051 0.7655 0.0014 0.35052046 596 2.4195 0.0041 1.0462 0.0134 0.0000 0.0058 0.9032 0.0015 0.39052047 569 1.8637 0.0033 0.8291 0.0126 0.0000 0.0056 0.9421 0.0017 0.41912048 522 1.8195 0.0035 0.8507 0.0115 0.0000 0.0054 0.6863 0.0013 0.32092049 539 1.8527 0.0034 0.8592 0.0114 0.0000 0.0053 0.6996 0.0013 0.32442050 553 1.8160 0.0033 0.9012 0.0143 0.0000 0.0071 0.7683 0.0014 0.38132051 556 1.8129 0.0033 0.8528 0.0151 0.0000 0.0071 0.8214 0.0015 0.3864-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 481APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------3026 470 1.7183 0.0037 0.8376 0.0110 0.0000 0.0054 0.6711 0.0014 0.32713027 560 1.7635 0.0031 0.8186 0.0088 0.0000 0.0041 0.8236 0.0015 0.38233028 629 1.9330 0.0031 0.9177 0.0164 0.0000 0.0078 0.7990 0.0013 0.37933029 578 2.1961 0.0038 1.0243 0.0048 0.0000 0.0022 0.7956 0.0014 0.37113030 612 1.7779 0.0029 0.8202 0.0148 0.0000 0.0068 0.9166 0.0015 0.42283031 509 1.8567 0.0036 0.8330 0.0135 0.0000 0.0061 0.8177 0.0016 0.36683032 492 1.7313 0.0035 0.8322 0.0113 0.0000 0.0054 0.8392 0.0017 0.40343033 503 1.7909 0.0036 0.8164 0.0100 0.0000 0.0046 0.5691 0.0011 0.25943034 583 1.7033 0.0029 0.8665 0.0160 0.0000 0.0081 0.7715 0.0013 0.39253035 480 1.6747 0.0035 0.8304 0.0091 0.0000 0.0045 0.7932 0.0017 0.39333036 563 1.8271 0.0032 0.8435 0.0130 0.0000 0.0060 0.7930 0.0014 0.36613037 464 1.9074 0.0041 0.8528 0.0110 0.0000 0.0049 0.7645 0.0016 0.34183038 576 1.6950 0.0029 0.8155 0.0159 0.0000 0.0076 0.9165 0.0016 0.44093039 515 1.8226 0.0035 0.8486 0.0116 0.0000 0.0054 0.8370 0.0016 0.38973040 584 2.2045 0.0038 0.9847 0.0138 0.0000 0.0062 0.9701 0.0017 0.43333041 633 2.1469 0.0034 0.9453 0.0132 0.0000 0.0058 1.1318 0.0018 0.49833042 523 1.7223 0.0033 0.8493 0.0121 0.0000 0.0060 0.7340 0.0014 0.36203043 495 1.7071 0.0034 0.8143 0.0118 0.0000 0.0056 0.5375 0.0011 0.25643044 595 2.1811 0.0037 0.9827 0.0129 0.0000 0.0058 0.8552 0.0014 0.38533045 546 1.7942 0.0033 0.8013 0.0094 0.0000 0.0042 0.6157 0.0011 0.27503046 637 2.1426 0.0034 0.9483 0.0126 0.0000 0.0056 0.9975 0.0016 0.44153047 553 2.0711 0.0037 0.9699 0.0097 0.0000 0.0045 0.7999 0.0014 0.37463048 603 1.9389 0.0032 0.9011 0.0135 0.0000 0.0063 0.7877 0.0013 0.36613049 558 1.8626 0.0033 0.8894 0.0099 0.0000 0.0047 0.8947 0.0016 0.42723050 589 2.2451 0.0038 0.9924 0.0145 0.0000 0.0064 0.9580 0.0016 0.42353051 608 1.8680 0.0031 0.8436 0.0112 0.0000 0.0051 0.7961 0.0013 0.3595-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:41Huntingdon Life Sciences 00-4206 PAGE 482APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------4026 554 1.7862 0.0032 0.7720 0.0110 0.0000 0.0048 0.8137 0.0015 0.35174027 503 1.9986 0.0040 0.9299 0.0147 0.0000 0.0068 0.6680 0.0013 0.31084028 565 1.8421 0.0033 0.8933 0.0126 0.0000 0.0061 0.7723 0.0014 0.37454029 564 2.1121 0.0037 1.0373 0.0159 0.0000 0.0078 0.8917 0.0016 0.43794030 557 2.2741 0.0041 1.0779 0.0131 0.0000 0.0062 0.8875 0.0016 0.42074031 513 1.7523 0.0034 0.8351 0.0127 0.0000 0.0061 0.6539 0.0013 0.31164032 558 1.6215 0.0029 0.7384 0.0145 0.0000 0.0066 0.7545 0.0014 0.34364033 561 1.7530 0.0031 0.8582 0.0164 0.0000 0.0080 0.6664 0.0012 0.32634034 492 1.6287 0.0033 0.8131 0.0129 0.0000 0.0064 0.8213 0.0017 0.41004035 586 1.9064 0.0033 0.8404 0.0140 0.0000 0.0062 0.8296 0.0014 0.36574052 522 1.9100 0.0037 0.8980 0.0129 0.0000 0.0061 0.6865 0.0013 0.32284037 498 1.8465 0.0037 0.9035 0.0105 0.0000 0.0051 0.7646 0.0015 0.37414038 551 1.8696 0.0034 0.8441 0.0142 0.0000 0.0064 0.7459 0.0014 0.33674039 534 1.8699 0.0035 0.8676 0.0112 0.0000 0.0052 0.8789 0.0016 0.40784040 503 1.7503 0.0035 0.8463 0.0143 0.0000 0.0069 0.7595 0.0015 0.36724041 562 1.8181 0.0032 0.8871 0.0124 0.0000 0.0061 0.6229 0.0011 0.30394042 492 1.6220 0.0033 0.7987 0.0116 0.0000 0.0057 0.8098 0.0016 0.39884043 532 2.0453 0.0038 0.9798 0.0111 0.0000 0.0053 0.7608 0.0014 0.36454044 586 2.3214 0.0040 1.1301 0.0162 0.0000 0.0079 0.6029 0.0010 0.29354045 590 1.9521 0.0033 0.8592 0.0133 0.0000 0.0059 0.8167 0.0014 0.35954046 511 1.7747 0.0035 0.8411 0.0153 0.0000 0.0073 0.7520 0.0015 0.35644047 525 1.8851 0.0036 0.8961 0.0110 0.0000 0.0052 0.8364 0.0016 0.39764048 515 1.7573 0.0034 0.8062 0.0104 0.0000 0.0048 0.6338 0.0012 0.29084049 459 1.6718 0.0036 0.7999 0.0092 0.0000 0.0044 0.5985 0.0013 0.28634050 515 1.9732 0.0038 0.9737 0.0126 0.0000 0.0062 0.7831 0.0015 0.38644051 587 1.7222 0.0029 0.8199 0.0117 0.0000 0.0056 0.8046 0.0014 0.3831-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:43Huntingdon Life Sciences 00-4206 PAGE 483APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------OVARY RIGHT OVARY LEFT OVARIES UTERUS/OVIDUCTSAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------1526 319 0.0361 0.0001 0.0191 0.0554 0.0002 0.0294 0.0893 0.0003 0.0474 0.5245 0.0016 0.27821528 350 0.0509 0.0001 0.0230 0.0560 0.0002 0.0253 0.1080 0.0003 0.0488 0.6691 0.0019 0.30211529 PNMD 358 0.0580 0.0002 0.0262 0.0535 0.0001 0.0242 0.1069 0.0003 0.0483 0.7184 0.0020 0.32481530 341 0.0594 0.0002 0.0290 0.0619 0.0002 0.0302 0.1212 0.0004 0.0592 0.5966 0.0017 0.29131531 347 0.0817 0.0002 0.0384 0.0623 0.0002 0.0293 0.1399 0.0004 0.0657 0.5760 0.0017 0.27051533 339 0.0714 0.0002 0.0345 0.1611 0.0005 0.0777 0.2281 0.0007 0.1101 0.5591 0.0016 0.26981534 317 0.0513 0.0002 0.0279 0.0568 0.0002 0.0309 0.1071 0.0003 0.0583 0.9397 0.0030 0.51141536 322 0.0696 0.0002 0.0383 0.0590 0.0002 0.0325 0.1281 0.0004 0.0705 0.7344 0.0023 0.40441537 302 0.0908 0.0003 0.0470 0.0538 0.0002 0.0279 0.1361 0.0005 0.0705 0.5009 0.0017 0.25951538 320 0.0342 0.0001 0.0184 0.0367 0.0001 0.0198 0.0693 0.0002 0.0373 1.0990 0.0034 0.59161539 330 0.0779 0.0002 0.0381 0.0708 0.0002 0.0346 0.1469 0.0004 0.0718 0.7145 0.0022 0.34941540 320 0.0638 0.0002 0.0331 0.0555 0.0002 0.0288 0.1171 0.0004 0.0608 1.0916 0.0034 0.56701542 347 0.0585 0.0002 0.0302 0.0834 0.0002 0.0431 0.1387 0.0004 0.0716 0.9121 0.0026 0.47081543 320 0.0643 0.0002 0.0336 0.0647 0.0002 0.0338 0.1280 0.0004 0.0669 0.9897 0.0031 0.51721544 360 0.0545 0.0002 0.0276 0.0513 0.0001 0.0260 0.1042 0.0003 0.0528 0.5743 0.0016 0.29121545 381 0.0594 0.0002 0.0282 0.0565 0.0001 0.0268 0.1152 0.0003 0.0546 0.5912 0.0016 0.28031546 299 0.0537 0.0002 0.0275 0.0383 0.0001 0.0196 0.0892 0.0003 0.0456 0.5430 0.0018 0.27771547 309 0.0686 0.0002 0.0379 0.0480 0.0002 0.0265 0.1160 0.0004 0.0641 0.6849 0.0022 0.37841549 332 0.0800 0.0002 0.0399 0.0759 0.0002 0.0379 0.1517 0.0005 0.0757 0.5715 0.0017 0.28531551 286 0.0638 0.0002 0.0345 0.0550 0.0002 0.0297 0.1169 0.0004 0.0632 0.7763 0.0027 0.4197-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTPNMD=PREGNANT, NO MATING DATE23-JUN-2009 20:43Huntingdon Life Sciences 00-4206 PAGE 484APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------OVARY RIGHT OVARY LEFT OVARIES UTERUS/OVIDUCTSAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------2526 320 0.0607 0.0002 0.0309 0.0591 0.0002 0.0301 0.1159 0.0004 0.0590 0.6193 0.0019 0.31522528 315 0.0547 0.0002 0.0292 0.0657 0.0002 0.0351 0.1041 0.0003 0.0557 0.5201 0.0017 0.27802529 348 0.0565 0.0002 0.0272 0.0501 0.0001 0.0242 0.1075 0.0003 0.0518 0.4817 0.0014 0.23222530 371 0.0485 0.0001 0.0228 0.0455 0.0001 0.0214 0.0990 0.0003 0.0465 0.6215 0.0017 0.29222531 310 0.0526 0.0002 0.0277 0.0462 0.0001 0.0244 0.0964 0.0003 0.0508 0.9657 0.0031 0.50942533 334 0.0699 0.0002 0.0349 0.0600 0.0002 0.0300 0.1267 0.0004 0.0633 0.5117 0.0015 0.25572534 295 0.0737 0.0002 0.0369 0.0702 0.0002 0.0351 0.1439 0.0005 0.0720 0.6088 0.0021 0.30482535 349 0.0561 0.0002 0.0305 0.0444 0.0001 0.0242 0.1007 0.0003 0.0548 0.4523 0.0013 0.24612536 338 0.0701 0.0002 0.0341 0.0548 0.0002 0.0267 0.1260 0.0004 0.0613 0.4528 0.0013 0.22032537 308 0.0649 0.0002 0.0345 0.0483 0.0002 0.0257 0.1116 0.0004 0.0593 0.7499 0.0024 0.39862539 344 0.0572 0.0002 0.0304 0.0563 0.0002 0.0299 0.1120 0.0003 0.0596 0.4681 0.0014 0.24902540 316 0.0497 0.0002 0.0249 0.0416 0.0001 0.0208 0.0907 0.0003 0.0454 0.5177 0.0016 0.25892541 392 0.0858 0.0002 0.0446 0.0505 0.0001 0.0263 0.1338 0.0003 0.0696 0.5874 0.0015 0.30552543 343 0.0821 0.0002 0.0411 0.0763 0.0002 0.0382 0.1554 0.0005 0.0777 0.5691 0.0017 0.28472544 337 0.0576 0.0002 0.0279 0.0445 0.0001 0.0216 0.1016 0.0003 0.0493 1.2428 0.0037 0.60272545 319 0.0877 0.0003 0.0436 0.0829 0.0003 0.0412 0.1700 0.0005 0.0846 0.7208 0.0023 0.35862546 292 0.0513 0.0002 0.0259 0.0627 0.0002 0.0317 0.1144 0.0004 0.0578 0.9454 0.0032 0.47752547 286 0.0301 0.0001 0.0159 0.0332 0.0001 0.0175 0.0663 0.0002 0.0350 0.5510 0.0019 0.29122548 342 0.0517 0.0002 0.0263 0.0545 0.0002 0.0278 0.1049 0.0003 0.0534 0.5308 0.0016 0.27042550 325 0.0663 0.0002 0.0344 0.0574 0.0002 0.0298 0.1239 0.0004 0.0643 0.6278 0.0019 0.32562551 333 0.0616 0.0002 0.0303 0.0554 0.0002 0.0272 0.1154 0.0003 0.0567 0.7515 0.0023 0.3694-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 485APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------OVARY RIGHT OVARY LEFT OVARIES UTERUS/OVIDUCTSAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------3527 355 0.0911 0.0003 0.0432 0.0890 0.0003 0.0422 0.1780 0.0005 0.0843 0.6203 0.0017 0.29393528 307 0.0390 0.0001 0.0206 0.0435 0.0001 0.0230 0.0830 0.0003 0.0439 0.5311 0.0017 0.28103529 305 0.0563 0.0002 0.0291 0.0478 0.0002 0.0247 0.1057 0.0003 0.0547 0.7288 0.0024 0.37723530 325 0.0595 0.0002 0.0310 0.0625 0.0002 0.0325 0.1222 0.0004 0.0636 0.4169 0.0013 0.21693531 318 0.0736 0.0002 0.0393 0.0568 0.0002 0.0303 0.1295 0.0004 0.0692 0.5599 0.0018 0.29913532 362 0.0570 0.0002 0.0292 0.0657 0.0002 0.0337 0.1198 0.0003 0.0614 0.4499 0.0012 0.23073533 357 0.0774 0.0002 0.0390 0.0872 0.0002 0.0439 0.1658 0.0005 0.0835 0.4130 0.0012 0.20793534 313 0.0882 0.0003 0.0458 0.0714 0.0002 0.0370 0.1569 0.0005 0.0814 1.0475 0.0033 0.54343535 345 0.0462 0.0001 0.0236 0.0489 0.0001 0.0250 0.0961 0.0003 0.0492 0.4499 0.0013 0.23023537 312 0.0592 0.0002 0.0295 0.0434 0.0001 0.0217 0.1032 0.0003 0.0515 0.5341 0.0017 0.26663540 321 0.0642 0.0002 0.0319 0.0571 0.0002 0.0283 0.1228 0.0004 0.0609 0.6667 0.0021 0.33083541 326 0.0673 0.0002 0.0369 0.0570 0.0002 0.0312 0.1224 0.0004 0.0671 0.4490 0.0014 0.24613542 355 0.0632 0.0002 0.0299 0.0494 0.0001 0.0234 0.1117 0.0003 0.0529 0.6632 0.0019 0.31383543 310 0.0534 0.0002 0.0276 0.0545 0.0002 0.0282 0.1065 0.0003 0.0551 0.8786 0.0028 0.45483544 304 0.0585 0.0002 0.0293 0.0434 0.0001 0.0218 0.1017 0.0003 0.0510 1.0545 0.0035 0.52853545 326 0.0556 0.0002 0.0295 0.0557 0.0002 0.0295 0.1099 0.0003 0.0582 0.5160 0.0016 0.27343546 345 0.0632 0.0002 0.0321 0.0485 0.0001 0.0246 0.1136 0.0003 0.0577 0.5364 0.0016 0.27263547 324 0.0651 0.0002 0.0337 0.0742 0.0002 0.0384 0.1366 0.0004 0.0707 0.5587 0.0017 0.28913549 329 0.0731 0.0002 0.0354 0.0742 0.0002 0.0360 0.1459 0.0004 0.0707 0.6046 0.0018 0.29313551 337 0.0688 0.0002 0.0344 0.0768 0.0002 0.0384 0.1452 0.0004 0.0725 0.7778 0.0023 0.3884-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 486APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------OVARY RIGHT OVARY LEFT OVARIES UTERUS/OVIDUCTSAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------4526 292 0.0414 0.0001 0.0201 0.0488 0.0002 0.0237 0.0898 0.0003 0.0436 0.4836 0.0017 0.23514527 348 0.0604 0.0002 0.0280 0.0636 0.0002 0.0295 0.1229 0.0004 0.0570 0.6306 0.0018 0.29234528 336 0.0589 0.0002 0.0297 0.0402 0.0001 0.0203 0.0991 0.0003 0.0500 0.4494 0.0013 0.22674529 324 0.0732 0.0002 0.0374 0.0665 0.0002 0.0340 0.1375 0.0004 0.0703 0.9971 0.0031 0.51004531 318 0.0479 0.0002 0.0254 0.0408 0.0001 0.0216 0.0862 0.0003 0.0457 0.4396 0.0014 0.23304532 321 0.0378 0.0001 0.0186 0.0196 0.0001 0.0096 0.1027 0.0003 0.0504 0.5414 0.0017 0.26594533 359 0.0436 0.0001 0.0225 0.0517 0.0001 0.0267 0.0968 0.0003 0.0499 0.5021 0.0014 0.25904534 330 0.0438 0.0001 0.0225 0.0646 0.0002 0.0332 0.1080 0.0003 0.0555 1.0670 0.0032 0.54784535 277 0.0450 0.0002 0.0233 0.0483 0.0002 0.0250 0.0864 0.0003 0.0447 0.7424 0.0027 0.38404536 321 0.0799 0.0002 0.0381 0.0749 0.0002 0.0357 0.1511 0.0005 0.0720 0.5337 0.0017 0.25424537 361 0.0521 0.0001 0.0268 0.0714 0.0002 0.0367 0.1265 0.0004 0.0651 0.4286 0.0012 0.22054538 291 0.0567 0.0002 0.0291 0.0532 0.0002 0.0273 0.1098 0.0004 0.0564 0.9779 0.0034 0.50224540 304 0.0369 0.0001 0.0194 0.0528 0.0002 0.0277 0.0884 0.0003 0.0465 0.5491 0.0018 0.28854542 314 0.0577 0.0002 0.0311 0.0552 0.0002 0.0298 0.1094 0.0003 0.0590 0.4888 0.0016 0.26354543 350 0.0779 0.0002 0.0412 0.0502 0.0001 0.0265 0.1266 0.0004 0.0669 0.6563 0.0019 0.34704544 303 0.0483 0.0002 0.0246 0.0586 0.0002 0.0298 0.1044 0.0003 0.0531 0.9878 0.0033 0.50264545 322 0.0621 0.0002 0.0306 0.0483 0.0002 0.0238 0.1178 0.0004 0.0581 0.8326 0.0026 0.41064546 333 0.0565 0.0002 0.0271 0.0590 0.0002 0.0283 0.1119 0.0003 0.0536 0.7698 0.0023 0.36894547 PNMD 325 0.0462 0.0001 0.0244 0.0638 0.0002 0.0337 0.1107 0.0003 0.0585 1.1007 0.0034 0.58194548 349 0.0510 0.0001 0.0266 0.0648 0.0002 0.0338 0.1151 0.0003 0.0600 0.4625 0.0013 0.24114550 359 0.0435 0.0001 0.0211 0.0505 0.0001 0.0244 0.1041 0.0003 0.0504 0.6736 0.0019 0.32614551 317 0.0622 0.0002 0.0342 0.0390 0.0001 0.0215 0.1084 0.0003 0.0596 0.6258 0.0020 0.3443-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTPNMD=PREGNANT, NO MATING DATE23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 487APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------1526 319 0.0797 0.0002 0.0423 1.8855 0.0059 1.0000 2.5994 0.0081 1.3786 15.9652 0.0500 8.46741528 350 0.0892 0.0003 0.0403 2.2149 0.0063 1.0000 3.0228 0.0086 1.3648 15.5199 0.0443 7.00701529 PNMD 358 0.0712 0.0002 0.0322 2.2118 0.0062 1.0000 2.9845 0.0083 1.3494 16.6851 0.0466 7.54371530 341 0.0858 0.0003 0.0419 2.0481 0.0060 1.0000 3.1346 0.0092 1.5305 17.1079 0.0501 8.35311531 347 0.0694 0.0002 0.0326 2.1290 0.0061 1.0000 2.9063 0.0084 1.3651 13.8805 0.0400 6.51971533 339 0.0805 0.0002 0.0388 2.0725 0.0061 1.0000 2.8494 0.0084 1.3749 18.0052 0.0531 8.68771534 317 0.0836 0.0003 0.0455 1.8375 0.0058 1.0000 2.7259 0.0086 1.4835 14.3507 0.0452 7.80991536 322 0.0844 0.0003 0.0465 1.8161 0.0056 1.0000 2.6384 0.0082 1.4528 13.9716 0.0434 7.69321537 302 0.0989 0.0003 0.0512 1.9305 0.0064 1.0000 2.8796 0.0095 1.4916 12.8743 0.0426 6.66891538 320 0.0799 0.0002 0.0430 1.8577 0.0058 1.0000 2.5295 0.0079 1.3616 15.2784 0.0477 8.22441539 330 0.0797 0.0002 0.0390 2.0452 0.0062 1.0000 2.8847 0.0088 1.4105 14.7976 0.0449 7.23531540 320 0.0935 0.0003 0.0486 1.9252 0.0060 1.0000 2.7780 0.0087 1.4430 15.0931 0.0471 7.83981542 347 0.0961 0.0003 0.0496 1.9372 0.0056 1.0000 14.4597 0.0417 7.4642 16.8785 0.0487 8.71281543 320 0.0834 0.0003 0.0436 1.9136 0.0060 1.0000 2.6349 0.0082 1.3769 14.9966 0.0469 7.83691544 360 0.0895 0.0002 0.0454 1.9723 0.0055 1.0000 2.8769 0.0080 1.4587 17.8918 0.0497 9.07151545 381 0.0784 0.0002 0.0372 2.1095 0.0055 1.0000 2.8856 0.0076 1.3679 18.2400 0.0478 8.64661546 299 0.0790 0.0003 0.0404 1.9550 0.0065 1.0000 2.2913 0.0077 1.1720 13.0830 0.0438 6.69211547 309 0.0793 0.0003 0.0438 1.8102 0.0059 1.0000 2.4128 0.0078 1.3329 13.9238 0.0451 7.69191549 332 0.0811 0.0002 0.0405 2.0031 0.0060 1.0000 2.9424 0.0089 1.4689 14.3955 0.0434 7.18661551 286 0.0819 0.0003 0.0443 1.8496 0.0065 1.0000 2.4848 0.0087 1.3434 11.9407 0.0418 6.4558-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTPNMD=PREGNANT, NO MATING DATE23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 488APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------2526 320 0.0923 0.0003 0.0470 1.9648 0.0061 1.0000 2.5608 0.0080 1.3033 14.8140 0.0463 7.53972528 315 0.0665 0.0002 0.0356 1.8706 0.0059 1.0000 2.6440 0.0084 1.4135 15.0611 0.0479 8.05152529 348 0.0892 0.0003 0.0430 2.0742 0.0060 1.0000 3.1762 0.0091 1.5313 17.9404 0.0516 8.64932530 371 0.0764 0.0002 0.0359 2.1273 0.0057 1.0000 2.6547 0.0071 1.2479 15.5799 0.0420 7.32382531 310 0.0710 0.0002 0.0374 1.8959 0.0061 1.0000 2.5763 0.0083 1.3589 14.4542 0.0466 7.62392533 334 0.0734 0.0002 0.0367 2.0013 0.0060 1.0000 2.5590 0.0077 1.2787 15.1291 0.0453 7.55962534 295 0.0945 0.0003 0.0473 1.9976 0.0068 1.0000 3.1052 0.0105 1.5545 13.0412 0.0442 6.52842535 349 0.0852 0.0002 0.0464 1.8378 0.0053 1.0000 2.9100 0.0083 1.5834 17.2314 0.0494 9.37612536 338 0.0683 0.0002 0.0332 2.0552 0.0061 1.0000 3.1500 0.0093 1.5327 15.8841 0.0470 7.72872537 308 0.0735 0.0002 0.0391 1.8813 0.0061 1.0000 2.5923 0.0084 1.3779 14.1336 0.0459 7.51272539 344 0.0870 0.0003 0.0463 1.8800 0.0055 1.0000 3.0654 0.0089 1.6305 16.7921 0.0488 8.93202540 316 0.0605 0.0002 0.0303 1.9997 0.0063 1.0000 2.4494 0.0078 1.2249 14.1950 0.0449 7.09862541 392 0.0843 0.0002 0.0438 1.9229 0.0049 1.0000 3.3702 0.0086 1.7527 20.4544 0.0522 10.63732543 343 0.0847 0.0002 0.0424 1.9989 0.0058 1.0000 3.1425 0.0092 1.5721 17.1332 0.0499 8.57132544 337 0.0839 0.0002 0.0407 2.0620 0.0061 1.0000 3.0825 0.0091 1.4949 13.3880 0.0397 6.49272545 319 0.1196 0.0004 0.0595 2.0102 0.0063 1.0000 2.9113 0.0091 1.4483 16.4452 0.0516 8.18092546 292 0.0807 0.0003 0.0408 1.9798 0.0068 1.0000 2.4123 0.0083 1.2185 13.8196 0.0474 6.98032547 286 0.0618 0.0002 0.0327 1.8924 0.0066 1.0000 2.0980 0.0073 1.1086 11.6273 0.0407 6.14422548 342 0.0752 0.0002 0.0383 1.9631 0.0057 1.0000 2.8647 0.0084 1.4593 18.7377 0.0547 9.54502550 325 0.0727 0.0002 0.0377 1.9280 0.0059 1.0000 2.6098 0.0080 1.3536 15.4699 0.0476 8.02382551 333 0.0811 0.0002 0.0399 2.0344 0.0061 1.0000 2.9879 0.0090 1.4687 19.4564 0.0584 9.5637-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 489APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------3527 355 0.0877 0.0002 0.0416 2.1104 0.0059 1.0000 2.9815 0.0084 1.4128 16.2401 0.0458 7.69533528 307 0.0634 0.0002 0.0335 1.8898 0.0061 1.0000 2.5007 0.0081 1.3233 12.8494 0.0418 6.79933529 305 0.0810 0.0003 0.0419 1.9321 0.0063 1.0000 3.0248 0.0099 1.5656 15.2935 0.0501 7.91553530 325 0.0662 0.0002 0.0344 1.9218 0.0059 1.0000 2.9210 0.0090 1.5199 15.4342 0.0474 8.03113531 318 0.0673 0.0002 0.0360 1.8717 0.0059 1.0000 2.6510 0.0083 1.4164 14.2479 0.0449 7.61233532 362 0.0737 0.0002 0.0378 1.9498 0.0054 1.0000 2.8674 0.0079 1.4706 16.2160 0.0448 8.31683533 357 0.0970 0.0003 0.0488 1.9864 0.0056 1.0000 2.8448 0.0080 1.4321 18.1003 0.0507 9.11213534 313 0.1018 0.0003 0.0528 1.9276 0.0062 1.0000 2.9026 0.0093 1.5058 13.3284 0.0426 6.91453535 345 0.0695 0.0002 0.0356 1.9547 0.0057 1.0000 2.7409 0.0080 1.4022 16.4886 0.0478 8.43543537 312 0.0793 0.0003 0.0396 2.0035 0.0064 1.0000 2.5365 0.0081 1.2660 14.4783 0.0464 7.22653540 321 0.0976 0.0003 0.0484 2.0154 0.0063 1.0000 2.7446 0.0085 1.3618 15.0811 0.0469 7.48293541 326 0.0714 0.0002 0.0391 1.8242 0.0056 1.0000 2.8925 0.0089 1.5856 15.3179 0.0470 8.39713542 355 0.0861 0.0002 0.0407 2.1134 0.0059 1.0000 2.9229 0.0082 1.3830 16.3857 0.0461 7.75323543 310 0.0979 0.0003 0.0507 1.9318 0.0062 1.0000 2.8065 0.0091 1.4528 12.0504 0.0389 6.23793544 304 0.0728 0.0002 0.0365 1.9951 0.0066 1.0000 3.0668 0.0101 1.5372 15.1003 0.0496 7.56873545 326 0.0887 0.0003 0.0470 1.8871 0.0058 1.0000 2.7984 0.0086 1.4829 15.8975 0.0487 8.42433546 345 0.0674 0.0002 0.0342 1.9679 0.0057 1.0000 2.9546 0.0086 1.5014 19.2074 0.0558 9.76043547 324 0.0911 0.0003 0.0471 1.9327 0.0060 1.0000 2.8802 0.0089 1.4902 16.9666 0.0524 8.77873549 329 0.0779 0.0002 0.0378 2.0627 0.0063 1.0000 2.9898 0.0091 1.4495 17.0718 0.0519 8.27643551 337 0.0806 0.0002 0.0402 2.0026 0.0059 1.0000 3.0820 0.0091 1.5390 15.7045 0.0466 7.8421-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 490APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------ADRENALS BRAIN KIDNEYS LIVERAnimal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g g-----------------------------------------------------------------------------------------------------------------------------------4526 292 0.0779 0.0003 0.0379 2.0574 0.0070 1.0000 2.4938 0.0085 1.2121 13.0099 0.0446 6.32354527 348 0.0915 0.0003 0.0424 2.1576 0.0062 1.0000 3.3619 0.0097 1.5582 18.3593 0.0528 8.50914528 336 0.0731 0.0002 0.0369 1.9822 0.0059 1.0000 2.8423 0.0085 1.4339 15.7148 0.0468 7.92804529 324 0.0696 0.0002 0.0356 1.9550 0.0060 1.0000 3.0380 0.0094 1.5540 15.9810 0.0494 8.17444531 318 0.0827 0.0003 0.0438 1.8867 0.0059 1.0000 2.5675 0.0081 1.3608 15.5333 0.0488 8.23314532 321 0.0707 0.0002 0.0347 2.0364 0.0064 1.0000 2.6582 0.0083 1.3053 15.7336 0.0491 7.72624533 359 0.0853 0.0002 0.0440 1.9383 0.0054 1.0000 3.0342 0.0085 1.5654 18.3623 0.0512 9.47344534 330 0.0833 0.0003 0.0428 1.9477 0.0059 1.0000 2.9925 0.0091 1.5364 15.8291 0.0480 8.12714535 277 0.0756 0.0003 0.0391 1.9333 0.0070 1.0000 2.7033 0.0098 1.3983 12.9745 0.0469 6.71114536 321 0.0851 0.0003 0.0405 2.0993 0.0065 1.0000 2.6610 0.0083 1.2676 19.1754 0.0598 9.13424537 361 0.1116 0.0003 0.0574 1.9440 0.0054 1.0000 3.1030 0.0086 1.5962 19.2288 0.0533 9.89144538 291 0.0955 0.0003 0.0490 1.9472 0.0067 1.0000 2.8642 0.0098 1.4709 13.3085 0.0458 6.83474540 304 0.0671 0.0002 0.0353 1.9031 0.0063 1.0000 2.6022 0.0086 1.3673 14.4358 0.0475 7.58544542 314 0.5661 0.0018 0.3052 1.8549 0.0059 1.0000 2.9713 0.0095 1.6019 15.6630 0.0498 8.44414543 350 0.0820 0.0002 0.0434 1.8913 0.0054 1.0000 2.8672 0.0082 1.5160 18.8812 0.0540 9.98324544 303 0.0812 0.0003 0.0413 1.9652 0.0065 1.0000 2.9504 0.0097 1.5013 15.0090 0.0495 7.63744545 322 0.0730 0.0002 0.0360 2.0279 0.0063 1.0000 3.1900 0.0099 1.5731 15.5356 0.0483 7.66094546 333 0.1000 0.0003 0.0479 2.0865 0.0063 1.0000 3.0054 0.0090 1.4404 18.4687 0.0554 8.85154547 PNMD 325 0.0711 0.0002 0.0376 1.8916 0.0058 1.0000 3.1696 0.0097 1.6756 17.7000 0.0544 9.35724548 349 0.0986 0.0003 0.0514 1.9179 0.0055 1.0000 3.1811 0.0091 1.6586 18.0740 0.0518 9.42384550 359 0.0896 0.0002 0.0434 2.0657 0.0057 1.0000 3.2526 0.0091 1.5746 18.0449 0.0502 8.73554551 317 0.0796 0.0003 0.0438 1.8178 0.0057 1.0000 3.1310 0.0099 1.7224 16.0040 0.0505 8.8040-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTPNMD=PREGNANT, NO MATING DATE23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 491APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP I 0 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------1526 319 1.3591 0.0043 0.7208 0.0116 0.0000 0.0062 0.7950 0.0025 0.42161528 350 1.7753 0.0051 0.8015 0.0117 0.0000 0.0053 0.7311 0.0021 0.33011529 PNMD 358 1.6839 0.0047 0.7613 0.0114 0.0000 0.0052 0.7129 0.0020 0.32231530 341 1.6074 0.0047 0.7848 0.0156 0.0000 0.0076 0.8858 0.0026 0.43251531 347 1.4982 0.0043 0.7037 0.0150 0.0000 0.0070 0.7081 0.0020 0.33261533 339 1.9369 0.0057 0.9346 0.0144 0.0000 0.0069 0.7417 0.0022 0.35791534 317 1.5106 0.0048 0.8221 0.0165 0.0001 0.0090 0.6681 0.0021 0.36361536 322 1.2301 0.0038 0.6773 0.0166 0.0001 0.0091 0.6222 0.0019 0.34261537 302 1.5359 0.0051 0.7956 0.0140 0.0000 0.0073 0.6294 0.0021 0.32601538 320 1.3536 0.0042 0.7286 0.0153 0.0000 0.0082 0.5974 0.0019 0.32161539 330 1.7169 0.0052 0.8395 0.0166 0.0001 0.0081 0.8462 0.0026 0.41371540 320 1.5956 0.0050 0.8288 0.0136 0.0000 0.0071 0.6587 0.0021 0.34211542 347 1.6463 0.0047 0.8498 0.0131 0.0000 0.0068 0.9854 0.0028 0.50871543 320 1.4105 0.0044 0.7371 0.0135 0.0000 0.0071 0.6787 0.0021 0.35471544 360 1.5207 0.0042 0.7710 0.0162 0.0000 0.0082 0.7281 0.0020 0.36921545 381 1.5751 0.0041 0.7467 0.0157 0.0000 0.0074 0.7540 0.0020 0.35741546 299 1.5459 0.0052 0.7907 0.0106 0.0000 0.0054 0.7774 0.0026 0.39761547 309 1.3357 0.0043 0.7379 0.0131 0.0000 0.0072 0.5591 0.0018 0.30891549 332 1.6857 0.0051 0.8415 0.0086 0.0000 0.0043 0.5718 0.0017 0.28551551 286 1.3778 0.0048 0.7449 0.0181 0.0001 0.0098 0.5961 0.0021 0.3223-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTPNMD=PREGNANT, NO MATING DATE23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 492APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP II 2000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------2526 320 1.5004 0.0047 0.7636 0.0176 0.0001 0.0090 0.5283 0.0017 0.26892528 315 1.5037 0.0048 0.8039 0.0131 0.0000 0.0070 0.6025 0.0019 0.32212529 348 1.6356 0.0047 0.7885 0.0118 0.0000 0.0057 0.7276 0.0021 0.35082530 371 1.6208 0.0044 0.7619 0.0137 0.0000 0.0064 0.5416 0.0015 0.25462531 310 1.6434 0.0053 0.8668 0.0155 0.0000 0.0082 0.7439 0.0024 0.39242533 334 1.5393 0.0046 0.7692 0.0161 0.0000 0.0080 0.5957 0.0018 0.29772534 295 1.6789 0.0057 0.8405 0.0159 0.0001 0.0080 0.5153 0.0017 0.25802535 349 1.7536 0.0050 0.9542 0.0108 0.0000 0.0059 0.6562 0.0019 0.35712536 338 1.4730 0.0044 0.7167 0.0123 0.0000 0.0060 0.6362 0.0019 0.30962537 308 1.5744 0.0051 0.8369 0.0148 0.0000 0.0079 0.4934 0.0016 0.26232539 344 1.6275 0.0047 0.8657 0.0184 0.0001 0.0098 0.6572 0.0019 0.34962540 316 1.4192 0.0045 0.7097 0.0142 0.0000 0.0071 0.5383 0.0017 0.26922541 392 1.9041 0.0049 0.9902 0.0223 0.0001 0.0116 0.9280 0.0024 0.48262543 343 1.6752 0.0049 0.8381 0.0118 0.0000 0.0059 0.7869 0.0023 0.39372544 337 1.6066 0.0048 0.7791 0.0185 0.0001 0.0090 0.6668 0.0020 0.32342545 319 1.5440 0.0048 0.7681 0.0141 0.0000 0.0070 0.6414 0.0020 0.31912546 292 1.5170 0.0052 0.7662 0.0179 0.0001 0.0090 0.5453 0.0019 0.27542547 286 1.2356 0.0043 0.6529 0.0103 0.0000 0.0054 0.4857 0.0017 0.25672548 342 1.5003 0.0044 0.7643 0.0132 0.0000 0.0067 0.5746 0.0017 0.29272550 325 1.5010 0.0046 0.7785 0.0151 0.0000 0.0078 0.6181 0.0019 0.32062551 333 1.5263 0.0046 0.7502 0.0172 0.0001 0.0085 0.6552 0.0020 0.3221-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 493APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP III 10000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------3527 355 1.7438 0.0049 0.8263 0.0058 0.0000 0.0027 0.6935 0.0020 0.32863528 307 1.4060 0.0046 0.7440 0.0091 0.0000 0.0048 0.5148 0.0017 0.27243529 305 1.5504 0.0051 0.8024 0.0160 0.0001 0.0083 0.7111 0.0023 0.36803530 325 1.5954 0.0049 0.8302 0.0159 0.0000 0.0083 0.6143 0.0019 0.31963531 318 1.5147 0.0048 0.8093 0.0030 0.0000 0.0016 0.6419 0.0020 0.34303532 362 1.8452 0.0051 0.9464 0.0081 0.0000 0.0042 0.8715 0.0024 0.44703533 357 1.6252 0.0045 0.8182 0.0123 0.0000 0.0062 0.6495 0.0018 0.32703534 313 1.4891 0.0048 0.7725 0.0187 0.0001 0.0097 0.6075 0.0019 0.31523535 345 1.3353 0.0039 0.6831 0.0130 0.0000 0.0067 0.5742 0.0017 0.29383537 312 1.4879 0.0048 0.7427 0.0128 0.0000 0.0064 0.6773 0.0022 0.33813540 321 1.5540 0.0048 0.7711 0.0111 0.0000 0.0055 0.6196 0.0019 0.30743541 326 1.5029 0.0046 0.8239 0.0141 0.0000 0.0077 0.5714 0.0018 0.31323542 355 1.6227 0.0046 0.7678 0.0140 0.0000 0.0066 0.8477 0.0024 0.40113543 310 1.4825 0.0048 0.7674 0.0151 0.0000 0.0078 0.5341 0.0017 0.27653544 304 1.4301 0.0047 0.7168 0.0137 0.0000 0.0069 0.5683 0.0019 0.28483545 326 1.6313 0.0050 0.8644 0.0156 0.0000 0.0083 0.6818 0.0021 0.36133546 345 1.5147 0.0044 0.7697 0.0155 0.0000 0.0079 0.6342 0.0018 0.32233547 324 1.5820 0.0049 0.8185 0.0159 0.0000 0.0082 0.5541 0.0017 0.28673549 329 1.5177 0.0046 0.7358 0.0152 0.0000 0.0074 0.5214 0.0016 0.25283551 337 1.5234 0.0045 0.7607 0.0160 0.0000 0.0080 0.6370 0.0019 0.3181-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT23-JUN-2009 20:42Huntingdon Life Sciences 00-4206 PAGE 494APPENDIX YGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSORGAN AND FINAL BODY WEIGHTSFEMALES GROUP IV 20000 MG/M3-----------------------------------------------------------------------------------------------------------------------------------LUNGS PITUITARY SPLEENAnimal FINAL BODY ------------------------- ------------------------- -------------------------Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Braing g g g-----------------------------------------------------------------------------------------------------------------------------------4526 292 1.4390 0.0049 0.6994 0.0119 0.0000 0.0058 0.5538 0.0019 0.26924527 348 1.7250 0.0050 0.7995 0.0148 0.0000 0.0069 0.7691 0.0022 0.35654528 336 1.3700 0.0041 0.6912 0.0071 0.0000 0.0036 0.5424 0.0016 0.27364529 324 1.5672 0.0048 0.8016 0.0116 0.0000 0.0059 0.5796 0.0018 0.29654531 318 1.3459 0.0042 0.7134 0.0144 0.0000 0.0076 0.6365 0.0020 0.33744532 321 1.5009 0.0047 0.7370 0.0102 0.0000 0.0050 0.5041 0.0016 0.24754533 359 1.6258 0.0045 0.8388 0.0143 0.0000 0.0074 0.5640 0.0016 0.29104534 330 1.5198 0.0046 0.7803 0.0121 0.0000 0.0062 0.5780 0.0018 0.29684535 277 1.2940 0.0047 0.6693 0.0146 0.0001 0.0076 0.5542 0.0020 0.28674536 321 1.5455 0.0048 0.7362 0.0136 0.0000 0.0065 0.5123 0.0016 0.24404537 361 1.5636 0.0043 0.8043 0.0115 0.0000 0.0059 0.5744 0.0016 0.29554538 291 1.4900 0.0051 0.7652 0.0141 0.0000 0.0072 0.5983 0.0021 0.30734540 304 1.5125 0.0050 0.7948 0.0137 0.0000 0.0072 0.6391 0.0021 0.33584542 314 1.4750 0.0047 0.7952 0.0174 0.0001 0.0094 0.6495 0.0021 0.35024543 350 1.6689 0.0048 0.8824 0.0156 0.0000 0.0082 0.6530 0.0019 0.34534544 303 1.5542 0.0051 0.7909 0.0155 0.0001 0.0079 0.6200 0.0020 0.31554545 322 1.5360 0.0048 0.7574 0.0131 0.0000 0.0065 0.4642 0.0014 0.22894546 333 1.7892 0.0054 0.8575 0.0169 0.0001 0.0081 0.7455 0.0022 0.35734547 PNMD 325 1.4298 0.0044 0.7559 0.0123 0.0000 0.0065 0.7135 0.0022 0.37724548 349 1.5524 0.0045 0.8094 0.0168 0.0000 0.0088 0.6485 0.0019 0.33814550 359 1.4954 0.0042 0.7239 0.0122 0.0000 0.0059 0.5713 0.0016 0.27664551 317 1.4236 0.0045 0.7831 0.0132 0.0000 0.0073 0.5951 0.0019 0.3274-----------------------------------------------------------------------------------------------------------------------------------%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHTPNMD=PREGNANT, NO MATING DATE

# Huntingdon Life Sciences 00-4206 Page 495

211-TBA-1G Final Report

Individual Pup Organ Weights

Preface Appendix Z

Table of Contents

Organ Weights and Organ/Body Weight Ratios ..............................................................496

Organ Weights and Organ/ Brain Weight Ratios .............................................................51223-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 496APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP I 0 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1526 4 M 81.2 1.4662 1.8057 0.4375 0.5388 0.3820 0.47041528 7 M 75.3 1.4576 1.9357 0.2902 0.3854 0.3140 0.41701529 9 M 85.0 1.5902 1.8708 0.3638 0.4280 0.3794 0.44641530 6 M 81.4 1.4794 1.8174 0.3220 0.3956 0.2276 0.27961531 6 M 80.5 1.6676 2.0716 0.3587 0.4456 0.3317 0.41201533 8 M 76.5 1.5730 2.0562 0.3300 0.4314 0.4077 0.53291534 4 M 85.6 1.5476 1.8079 0.3381 0.3950 0.3183 0.37181536 4 M 58.9 1.3937 2.3662 0.2669 0.4531 0.2490 0.42281537 6 M 75.0 1.6971 2.2628 0.3518 0.4691 0.3689 0.49191538 3 M 85.1 1.6348 1.9210 0.3408 0.4005 0.4317 0.50731539 6 M 71.0 1.5964 2.2485 0.3018 0.4251 0.2845 0.40071540 1 M 94.1 1.5845 1.6838 0.4186 0.4448 0.4031 0.42841542 3 M 83.9 1.5730 1.8749 0.3482 0.4150 0.4631 0.55201543 4 M 100.3 1.5916 1.5868 0.3998 0.3986 0.4749 0.47351544 3 M 95.8 1.5413 1.6089 0.3117 0.3254 0.4063 0.42411545 8 M 78.6 1.6895 2.1495 0.3027 0.3851 0.3718 0.47301546 4 M 79.4 1.5294 1.9262 0.3805 0.4792 0.3716 0.468023-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 497APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP I 0 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1547 5 M 75.8 1.4437 1.9046 0.2423 0.3197 0.3477 0.45871549 1 M 63.9 1.3112 2.0520 0.2697 0.4221 0.2415 0.37791551 4 M 76.5 1.6021 2.0942 0.3852 0.5035 0.3655 0.477823-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 498APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP II 2000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2526 5 M 80.2 1.6586 2.0681 0.2658 0.3314 0.3872 0.48282528 2 M 82.3 1.5478 1.8807 0.2972 0.3611 0.4224 0.51322529 3 M 72.4 1.5318 2.1157 0.2763 0.3816 0.3112 0.42982530 2 M 102.3 1.5732 1.5378 0.3536 0.3457 0.4290 0.41942531 6 M 78.7 1.5021 1.9086 0.3855 0.4898 0.4383 0.55692533 1 M 79.5 1.5442 1.9424 0.3489 0.4389 0.3377 0.42482534 1 M 76.2 1.5038 1.9735 0.2791 0.3663 0.3251 0.42662535 9 M 71.2 1.5193 2.1338 0.3829 0.5378 0.3195 0.44872536 4 M 92.3 1.5042 1.6297 0.3411 0.3696 0.3112 0.33722537 2 M 71.3 1.5105 2.1185 0.3422 0.4799 0.3245 0.45512539 7 M 87.8 1.6699 1.9019 0.3168 0.3608 0.2477 0.28212540 2 M 82.9 1.6289 1.9649 0.3956 0.4772 0.3991 0.48142541 3 M 84.8 1.6642 1.9625 0.3794 0.4474 0.2777 0.32752543 3 M 69.5 1.5745 2.2655 0.2969 0.4272 0.2637 0.37942544 2 M 83.1 1.4789 1.7797 0.2593 0.3120 0.3362 0.40462545 4 M 77.2 1.5038 1.9479 0.3537 0.4582 0.3097 0.40122546 5 M 85.5 1.6632 1.9453 0.3795 0.4439 0.3267 0.382123-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 499APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP II 2000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2547 1 M 76.8 1.6349 2.1288 0.3199 0.4165 0.2786 0.36282548 4 M 77.8 1.5963 2.0518 0.4234 0.5442 0.3340 0.42932550 3 M 92.4 1.5981 1.7295 0.3502 0.3790 0.4045 0.43782551 1 M 96.3 1.6928 1.7578 0.2827 0.2936 0.2646 0.274823-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 500APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP III 10000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3527 7 M 85.9 1.5073 1.7547 0.3728 0.4340 0.3472 0.40423528 2 M 68.1 1.5068 2.2126 0.2470 0.3627 0.3633 0.53353529 7 M 70.5 1.5598 2.2125 0.3992 0.5662 0.3299 0.46793530 4 M 69.7 1.5995 2.2948 0.2594 0.3722 0.4297 0.61653531 8 M 82.2 1.5579 1.8953 0.3649 0.4439 0.3537 0.43033532 1 M 80.2 1.5923 1.9854 0.3791 0.4727 0.4028 0.50223533 7 M 83.1 1.6573 1.9943 0.3285 0.3953 0.4351 0.52363534 7 M 77.5 1.4399 1.8579 0.2819 0.3637 0.4220 0.54453535 4 M 75.8 1.6212 2.1388 0.2928 0.3863 0.3213 0.42393537 8 M 64.2 1.3576 2.1146 0.3257 0.5073 0.3139 0.48893540 2 M 70.9 1.5702 2.2147 0.2857 0.4030 0.3019 0.42583541 2 M 81.9 1.5827 1.9325 0.3044 0.3717 0.3573 0.43633542 5 M 86.8 1.6429 1.8927 0.4063 0.4681 0.3056 0.35213543 3 M 78.9 1.5605 1.9778 0.3281 0.4158 0.3397 0.43053544 3 M 78.7 1.6998 2.1598 0.3396 0.4315 0.4152 0.52763545 1 M 70.8 1.5110 2.1342 0.2724 0.3847 0.3294 0.46533546 1 M 88.5 1.6972 1.9177 0.3671 0.4148 0.3744 0.423123-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 501APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP III 10000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3547 8 M 73.2 1.5184 2.0743 0.3080 0.4208 0.3050 0.41673549 3 M 84.9 1.5786 1.8594 0.3492 0.4113 0.4245 0.50003551 4 M 102.6 1.6755 1.6330 0.4548 0.4433 0.5643 0.550023-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 502APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4526 4 M 69.5 1.5032 2.1629 0.2873 0.4134 0.3032 0.43634527 3 M 74.2 1.4992 2.0205 0.2866 0.3863 0.3303 0.44514528 4 M 75.2 1.5573 2.0709 0.3389 0.4507 0.3234 0.43014529 7 M 62.8 1.4582 2.3220 0.2406 0.3831 0.2605 0.41484531 2 M 65.8 1.5362 2.3347 0.2384 0.3623 0.2896 0.44014532 2 M 73.1 1.5373 2.1030 0.2342 0.3204 0.3216 0.43994533 5 M 84.8 1.5387 1.8145 0.2972 0.3505 0.4583 0.54044534 6 M 81.7 1.4248 1.7439 0.2755 0.3372 0.3027 0.37054535 1 M 81.4 1.5965 1.9613 0.3041 0.3736 0.3817 0.46894536 2 M 77.1 1.6120 2.0908 0.2132 0.2765 0.3172 0.41144537 3 M 74.3 1.4964 2.0140 0.2941 0.3958 0.2777 0.37384538 3 M 72.9 1.5527 2.1299 0.3418 0.4689 0.3554 0.48754540 3 M 66.1 1.4597 2.2083 0.2910 0.4402 0.3062 0.46324542 1 M 74.9 1.5602 2.0830 0.3493 0.4664 0.3338 0.44574543 4 M 77.3 1.4852 1.9213 0.2810 0.3635 0.2539 0.32854544 4 M 74.9 1.5156 2.0235 0.2466 0.3292 0.3058 0.40834545 10 M 81.6 1.5904 1.9490 0.3206 0.3929 0.3853 0.472223-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 503APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4546 7 M 77.6 1.5982 2.0595 0.3022 0.3894 0.3446 0.44414547 1 M 74.7 1.4433 1.9321 0.2829 0.3787 0.2999 0.40154548 5 M 84.3 1.6055 1.9045 0.3843 0.4559 0.3712 0.44034550 4 M 85.3 1.5156 1.7768 0.3387 0.3971 0.3490 0.40914551 1 M 69.7 1.5156 2.1745 0.3052 0.4379 0.3427 0.491723-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 504APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP I 0 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1526 10 F 73.9 1.5050 2.0365 0.3057 0.4137 0.3198 0.43271528 10 F 73.8 1.4975 2.0291 0.2533 0.3432 0.3190 0.43221529 11 F 78.2 1.5844 2.0261 0.2751 0.3518 0.3385 0.43291530 9 F 67.6 1.4427 2.1342 0.2728 0.4036 0.3266 0.48311531 8 F 67.5 1.5932 2.3603 0.2536 0.3757 0.3044 0.45101533 10 F 69.7 1.5287 2.1933 0.3086 0.4428 0.3539 0.50771534 10 F 72.6 1.4871 2.0483 0.2604 0.3587 0.3590 0.49451536 12 F 52.3 1.3412 2.5644 0.2239 0.4281 0.1947 0.37231537 13 F 69.1 1.5780 2.2836 0.3141 0.4546 0.3108 0.44981538 10 F 74.3 1.6453 2.2144 0.3453 0.4647 0.3881 0.52231539 9 F 78.8 1.6369 2.0773 0.2956 0.3751 0.3566 0.45251540 14 F 81.9 1.4941 1.8243 0.3834 0.4681 0.3602 0.43981542 13 F 70.6 1.5236 2.1581 0.3161 0.4477 0.3946 0.55891543 15 F 77.9 1.4437 1.8533 0.2840 0.3646 0.3296 0.42311544 10 F 82.4 1.5117 1.8346 0.2519 0.3057 0.4839 0.58731545 10 F 83.4 1.6269 1.9507 0.2948 0.3535 0.4087 0.49001546 11 F 77.3 1.4154 1.8310 0.3319 0.4294 0.3615 0.467723-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 505APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP I 0 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1547 13 F 73.7 1.4416 1.9560 0.3481 0.4723 0.3890 0.52781549 13 F 72.0 1.4848 2.0622 0.3687 0.5121 0.2723 0.37821551 6 F 80.0 1.5888 1.9860 0.3594 0.4492 0.3970 0.496323-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 506APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP II 2000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2526 13 F 77.9 1.5212 1.9528 0.2802 0.3597 0.4103 0.52672528 11 F 69.9 1.4666 2.0981 0.3052 0.4366 0.3699 0.52922529 12 F 77.1 1.4865 1.9280 0.2887 0.3744 0.3932 0.51002530 14 F 79.6 1.5197 1.9092 0.2592 0.3256 0.3699 0.46472531 12 F 70.5 1.4574 2.0672 0.3061 0.4342 0.3843 0.54512533 15 F 74.8 1.4805 1.9793 0.2798 0.3741 0.3771 0.50412534 6 F 74.9 1.4331 1.9134 0.2968 0.3963 0.3674 0.49052535 13 F 54.7 1.3557 2.4784 0.2304 0.4212 0.3047 0.55702536 16 F 75.5 1.4340 1.8993 0.2546 0.3372 0.2881 0.38162537 7 F 70.7 1.5016 2.1239 0.2816 0.3983 0.4132 0.58442539 9 F 73.8 1.3791 1.8687 0.2919 0.3955 0.3205 0.43432540 9 F 75.2 1.6194 2.1535 0.3625 0.4820 0.3642 0.48432541 8 F 81.1 1.6324 2.0128 0.3703 0.4566 0.4311 0.53162543 10 F 69.1 1.5538 2.2486 0.3396 0.4915 0.3618 0.52362544 3 F 76.0 1.4702 1.9345 0.2938 0.3866 0.3654 0.48082545 8 F 68.8 1.4535 2.1126 0.3321 0.4827 0.3538 0.51422546 13 F 67.7 1.4992 2.2145 0.2516 0.3716 0.2520 0.372223-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 507APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP II 2000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2547 9 F 78.0 1.5616 2.0021 0.3267 0.4188 0.3181 0.40782548 8 F 78.5 1.6389 2.0878 0.3490 0.4446 0.3600 0.45862550 8 F 82.9 1.5717 1.8959 0.3598 0.4340 0.3987 0.48092551 12 F 82.6 1.5823 1.9156 0.3266 0.3954 0.3946 0.477723-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 508APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP III 10000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3527 17 F 78.4 1.4353 1.8307 0.3123 0.3983 0.3991 0.50913528 12 F 61.8 1.4357 2.3231 0.2300 0.3722 0.3103 0.50213529 10 F 80.8 1.6082 1.9903 0.4290 0.5309 0.3172 0.39263530 9 F 71.9 1.5833 2.2021 0.2848 0.3961 0.4238 0.58943531 11 F 74.3 1.5203 2.0462 0.2528 0.3402 0.4032 0.54273532 11 F 72.4 1.4882 2.0555 0.3472 0.4796 0.3093 0.42723533 11 F 86.3 1.5715 1.8210 0.3271 0.3790 0.5430 0.62923534 8 F 76.5 1.4586 1.9067 0.3340 0.4366 0.3844 0.50253535 7 F 69.3 1.4507 2.0934 0.3344 0.4825 0.3139 0.45303537 12 F 63.3 1.4542 2.2973 0.2851 0.4504 0.3712 0.58643540 12 F 59.5 1.5167 2.5491 0.2512 0.4222 0.2793 0.46943541 8 F 74.8 1.4917 1.9943 0.3230 0.4318 0.4042 0.54043542 11 F 77.0 1.5517 2.0152 0.3293 0.4277 0.3808 0.49453543 4 F 69.4 1.5094 2.1749 0.2195 0.3163 0.3196 0.46053544 10 F 72.1 1.6425 2.2781 0.2762 0.3831 0.3566 0.49463545 14 F 64.9 1.5169 2.3373 0.2749 0.4236 0.3368 0.51903546 15 F 77.6 1.5901 2.0491 0.3030 0.3905 0.3732 0.480923-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 509APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP III 10000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3547 14 F 66.6 1.4402 2.1625 0.2427 0.3644 0.3143 0.47193549 11 F 80.2 1.5666 1.9534 0.3158 0.3938 0.4554 0.567823-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 510APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4526 15 F 58.5 1.4212 2.4294 0.2816 0.4814 0.3387 0.57904527 11 F 67.5 1.4317 2.1210 0.2417 0.3581 0.3008 0.44564528 12 F 63.8 1.5089 2.3650 0.2719 0.4262 0.3514 0.55084529 12 F 67.2 1.3413 1.9960 0.2294 0.3414 0.2726 0.40574531 8 F 66.5 1.4198 2.1350 0.2951 0.4438 0.3369 0.50664532 14 F 52.3 1.3287 2.5405 0.1691 0.3233 0.2382 0.45544533 13 F 78.4 1.4541 1.8547 0.2782 0.3548 0.4788 0.61074534 14 F 63.6 1.6255 2.5558 0.3514 0.5525 0.4048 0.63654535 6 F 59.6 1.3900 2.3322 0.2059 0.3455 0.2388 0.40074536 14 F 68.3 1.5484 2.2671 0.2536 0.3713 0.3801 0.55654537 11 F 85.8 1.5585 1.8164 0.2984 0.3478 0.4207 0.49034538 15 F 73.6 1.5644 2.1255 0.2617 0.3556 0.4235 0.57544540 11 F 65.6 1.4976 2.2829 0.2629 0.4008 0.3220 0.49094542 10 F 68.6 1.4896 2.1714 0.2468 0.3598 0.3337 0.48644543 7 F 76.4 1.4954 1.9573 0.3038 0.3976 0.4368 0.57174544 15 F 70.7 1.4402 2.0371 0.2363 0.3342 0.3187 0.45084545 13 F 75.8 1.5201 2.0054 0.2483 0.3276 0.3577 0.471923-JUN-2009 21:29Huntingdon Life Sciences 00-4206 PAGE 511APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------Final BRAIN SPLEEN THYMUSAnimal Pup Body -------------- -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4546 16 F 74.6 1.5655 2.0985 0.3002 0.4024 0.3597 0.48224547 7 F 63.2 1.3757 2.1767 0.2622 0.4149 0.3232 0.51144548 11 F 75.4 1.5421 2.0452 0.3167 0.4200 0.4014 0.53244550 15 F 66.3 1.4782 2.2296 0.2289 0.3452 0.3199 0.48254551 12 F 64.8 1.4172 2.1870 0.2550 0.3935 0.3410 0.526223-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 512APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP I 0 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1526 4 M 1.4662 0.4375 29.839 0.3820 26.0541528 7 M 1.4576 0.2902 19.909 0.3140 21.5421529 9 M 1.5902 0.3638 22.878 0.3794 23.8591530 6 M 1.4794 0.3220 21.766 0.2276 15.3851531 6 M 1.6676 0.3587 21.510 0.3317 19.8911533 8 M 1.5730 0.3300 20.979 0.4077 25.9191534 4 M 1.5476 0.3381 21.847 0.3183 20.5671536 4 M 1.3937 0.2669 19.150 0.2490 17.8661537 6 M 1.6971 0.3518 20.729 0.3689 21.7371538 3 M 1.6348 0.3408 20.847 0.4317 26.4071539 6 M 1.5964 0.3018 18.905 0.2845 17.8211540 1 M 1.5845 0.4186 26.418 0.4031 25.4401542 3 M 1.5730 0.3482 22.136 0.4631 29.4411543 4 M 1.5916 0.3998 25.119 0.4749 29.8381544 3 M 1.5413 0.3117 20.223 0.4063 26.3611545 8 M 1.6895 0.3027 17.917 0.3718 22.0071546 4 M 1.5294 0.3805 24.879 0.3716 24.29723-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 513APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP I 0 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1547 5 M 1.4437 0.2423 16.783 0.3477 24.0841549 1 M 1.3112 0.2697 20.569 0.2415 18.4181551 4 M 1.6021 0.3852 24.043 0.3655 22.81423-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 514APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP II 2000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2526 5 M 1.6586 0.2658 16.026 0.3872 23.3452528 2 M 1.5478 0.2972 19.201 0.4224 27.2902529 3 M 1.5318 0.2763 18.038 0.3112 20.3162530 2 M 1.5732 0.3536 22.476 0.4290 27.2692531 6 M 1.5021 0.3855 25.664 0.4383 29.1792533 1 M 1.5442 0.3489 22.594 0.3377 21.8692534 1 M 1.5038 0.2791 18.560 0.3251 21.6192535 9 M 1.5193 0.3829 25.202 0.3195 21.0292536 4 M 1.5042 0.3411 22.677 0.3112 20.6892537 2 M 1.5105 0.3422 22.655 0.3245 21.4832539 7 M 1.6699 0.3168 18.971 0.2477 14.8332540 2 M 1.6289 0.3956 24.286 0.3991 24.5012541 3 M 1.6642 0.3794 22.798 0.2777 16.6872543 3 M 1.5745 0.2969 18.857 0.2637 16.7482544 2 M 1.4789 0.2593 17.533 0.3362 22.7332545 4 M 1.5038 0.3537 23.520 0.3097 20.5942546 5 M 1.6632 0.3795 22.817 0.3267 19.64323-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 515APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP II 2000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2547 1 M 1.6349 0.3199 19.567 0.2786 17.0412548 4 M 1.5963 0.4234 26.524 0.3340 20.9232550 3 M 1.5981 0.3502 21.914 0.4045 25.3112551 1 M 1.6928 0.2827 16.700 0.2646 15.63123-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 516APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP III 10000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3527 7 M 1.5073 0.3728 24.733 0.3472 23.0353528 2 M 1.5068 0.2470 16.392 0.3633 24.1113529 7 M 1.5598 0.3992 25.593 0.3299 21.1503530 4 M 1.5995 0.2594 16.218 0.4297 26.8653531 8 M 1.5579 0.3649 23.423 0.3537 22.7043532 1 M 1.5923 0.3791 23.808 0.4028 25.2973533 7 M 1.6573 0.3285 19.821 0.4351 26.2543534 7 M 1.4399 0.2819 19.578 0.4220 29.3083535 4 M 1.6212 0.2928 18.061 0.3213 19.8193537 8 M 1.3576 0.3257 23.991 0.3139 23.1223540 2 M 1.5702 0.2857 18.195 0.3019 19.2273541 2 M 1.5827 0.3044 19.233 0.3573 22.5753542 5 M 1.6429 0.4063 24.731 0.3056 18.6013543 3 M 1.5605 0.3281 21.025 0.3397 21.7693544 3 M 1.6998 0.3396 19.979 0.4152 24.4263545 1 M 1.5110 0.2724 18.028 0.3294 21.8003546 1 M 1.6972 0.3671 21.630 0.3744 22.06023-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 517APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP III 10000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3547 8 M 1.5184 0.3080 20.285 0.3050 20.0873549 3 M 1.5786 0.3492 22.121 0.4245 26.8913551 4 M 1.6755 0.4548 27.144 0.5643 33.67923-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 518APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4526 4 M 1.5032 0.2873 19.113 0.3032 20.1704527 3 M 1.4992 0.2866 19.117 0.3303 22.0324528 4 M 1.5573 0.3389 21.762 0.3234 20.7674529 7 M 1.4582 0.2406 16.500 0.2605 17.8644531 2 M 1.5362 0.2384 15.519 0.2896 18.8524532 2 M 1.5373 0.2342 15.235 0.3216 20.9204533 5 M 1.5387 0.2972 19.315 0.4583 29.7854534 6 M 1.4248 0.2755 19.336 0.3027 21.2454535 1 M 1.5965 0.3041 19.048 0.3817 23.9094536 2 M 1.6120 0.2132 13.226 0.3172 19.6774537 3 M 1.4964 0.2941 19.654 0.2777 18.5584538 3 M 1.5527 0.3418 22.013 0.3554 22.8894540 3 M 1.4597 0.2910 19.936 0.3062 20.9774542 1 M 1.5602 0.3493 22.388 0.3338 21.3954543 4 M 1.4852 0.2810 18.920 0.2539 17.0954544 4 M 1.5156 0.2466 16.271 0.3058 20.1774545 10 M 1.5904 0.3206 20.158 0.3853 24.22723-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 519APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 MALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4546 7 M 1.5982 0.3022 18.909 0.3446 21.5624547 1 M 1.4433 0.2829 19.601 0.2999 20.7794548 5 M 1.6055 0.3843 23.936 0.3712 23.1214550 4 M 1.5156 0.3387 22.348 0.3490 23.0274551 1 M 1.5156 0.3052 20.137 0.3427 22.61223-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 520APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP I 0 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1526 10 F 1.5050 0.3057 20.312 0.3198 21.2491528 10 F 1.4975 0.2533 16.915 0.3190 21.3021529 11 F 1.5844 0.2751 17.363 0.3385 21.3651530 9 F 1.4427 0.2728 18.909 0.3266 22.6381531 8 F 1.5932 0.2536 15.918 0.3044 19.1061533 10 F 1.5287 0.3086 20.187 0.3539 23.1501534 10 F 1.4871 0.2604 17.511 0.3590 24.1411536 12 F 1.3412 0.2239 16.694 0.1947 14.5171537 13 F 1.5780 0.3141 19.905 0.3108 19.6961538 10 F 1.6453 0.3453 20.987 0.3881 23.5881539 9 F 1.6369 0.2956 18.059 0.3566 21.7851540 14 F 1.4941 0.3834 25.661 0.3602 24.1081542 13 F 1.5236 0.3161 20.747 0.3946 25.8991543 15 F 1.4437 0.2840 19.672 0.3296 22.8301544 10 F 1.5117 0.2519 16.663 0.4839 32.0101545 10 F 1.6269 0.2948 18.120 0.4087 25.1211546 11 F 1.4154 0.3319 23.449 0.3615 25.54023-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 521APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP I 0 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------1547 13 F 1.4416 0.3481 24.147 0.3890 26.9841549 13 F 1.4848 0.3687 24.832 0.2723 18.3391551 6 F 1.5888 0.3594 22.621 0.3970 24.98723-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 522APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP II 2000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2526 13 F 1.5212 0.2802 18.420 0.4103 26.9722528 11 F 1.4666 0.3052 20.810 0.3699 25.2222529 12 F 1.4865 0.2887 19.421 0.3932 26.4512530 14 F 1.5197 0.2592 17.056 0.3699 24.3402531 12 F 1.4574 0.3061 21.003 0.3843 26.3692533 15 F 1.4805 0.2798 18.899 0.3771 25.4712534 6 F 1.4331 0.2968 20.710 0.3674 25.6372535 13 F 1.3557 0.2304 16.995 0.3047 22.4752536 16 F 1.4340 0.2546 17.755 0.2881 20.0912537 7 F 1.5016 0.2816 18.753 0.4132 27.5172539 9 F 1.3791 0.2919 21.166 0.3205 23.2402540 9 F 1.6194 0.3625 22.385 0.3642 22.4902541 8 F 1.6324 0.3703 22.684 0.4311 26.4092543 10 F 1.5538 0.3396 21.856 0.3618 23.2852544 3 F 1.4702 0.2938 19.984 0.3654 24.8542545 8 F 1.4535 0.3321 22.848 0.3538 24.3412546 13 F 1.4992 0.2516 16.782 0.2520 16.80923-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 523APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP II 2000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------2547 9 F 1.5616 0.3267 20.921 0.3181 20.3702548 8 F 1.6389 0.3490 21.295 0.3600 21.9662550 8 F 1.5717 0.3598 22.892 0.3987 25.3672551 12 F 1.5823 0.3266 20.641 0.3946 24.93823-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 524APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP III 10000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3527 17 F 1.4353 0.3123 21.759 0.3991 27.8063528 12 F 1.4357 0.2300 16.020 0.3103 21.6133529 10 F 1.6082 0.4290 26.676 0.3172 19.7243530 9 F 1.5833 0.2848 17.988 0.4238 26.7673531 11 F 1.5203 0.2528 16.628 0.4032 26.5213532 11 F 1.4882 0.3472 23.330 0.3093 20.7833533 11 F 1.5715 0.3271 20.815 0.5430 34.5533534 8 F 1.4586 0.3340 22.899 0.3844 26.3543535 7 F 1.4507 0.3344 23.051 0.3139 21.6383537 12 F 1.4542 0.2851 19.605 0.3712 25.5263540 12 F 1.5167 0.2512 16.562 0.2793 18.4153541 8 F 1.4917 0.3230 21.653 0.4042 27.0973542 11 F 1.5517 0.3293 21.222 0.3808 24.5413543 4 F 1.5094 0.2195 14.542 0.3196 21.1743544 10 F 1.6425 0.2762 16.816 0.3566 21.7113545 14 F 1.5169 0.2749 18.122 0.3368 22.2033546 15 F 1.5901 0.3030 19.055 0.3732 23.47023-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 525APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP III 10000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------3547 14 F 1.4402 0.2427 16.852 0.3143 21.8233549 11 F 1.5666 0.3158 20.158 0.4554 29.06923-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 526APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4526 15 F 1.4212 0.2816 19.814 0.3387 23.8324527 11 F 1.4317 0.2417 16.882 0.3008 21.0104528 12 F 1.5089 0.2719 18.020 0.3514 23.2884529 12 F 1.3413 0.2294 17.103 0.2726 20.3244531 8 F 1.4198 0.2951 20.785 0.3369 23.7294532 14 F 1.3287 0.1691 12.727 0.2382 17.9274533 13 F 1.4541 0.2782 19.132 0.4788 32.9284534 14 F 1.6255 0.3514 21.618 0.4048 24.9034535 6 F 1.3900 0.2059 14.813 0.2388 17.1804536 14 F 1.5484 0.2536 16.378 0.3801 24.5484537 11 F 1.5585 0.2984 19.147 0.4207 26.9944538 15 F 1.5644 0.2617 16.728 0.4235 27.0714540 11 F 1.4976 0.2629 17.555 0.3220 21.5014542 10 F 1.4896 0.2468 16.568 0.3337 22.4024543 7 F 1.4954 0.3038 20.316 0.4368 29.2104544 15 F 1.4402 0.2363 16.407 0.3187 22.1294545 13 F 1.5201 0.2483 16.334 0.3577 23.53123-JUN-2009 21:31Huntingdon Life Sciences 00-4206 PAGE 527APPENDIX ZGASOLINE TBA VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODYINHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%)GROUP IV 20000 MG/M3 FEMALE PUPS-----------------------------------------------------------------------------------------------------------------------------------SPLEEN THYMUSAnimal Pup Brain -------------- --------------Number No. Sex Weight Weight Ratio Weight Ratio-----------------------------------------------------------------------------------------------------------------------------------4546 16 F 1.5655 0.3002 19.176 0.3597 22.9774547 7 F 1.3757 0.2622 19.059 0.3232 23.4934548 11 F 1.5421 0.3167 20.537 0.4014 26.0294550 15 F 1.4782 0.2289 15.485 0.3199 21.6414551 12 F 1.4172 0.2550 17.993 0.3410 24.062

# Huntingdon Life Sciences 00-4206 Page 528

211-TBA-1G Final Report

Individual Animal Gross

and Microscopic Observations

Preface Appendix AA

Key to Abbreviations

Gl = Gland

LN = Lymph Node

Notes

1. Unless otherwise specified in a histopathology note, the organ/tissue exam ined was

the required (routine) section.

2. The dimensions of gross lesions are presen ted as a general range based on the actual

dimensions noted during macroscopic examin ations. The actual dim ensions of gross

lesions can be found in the raw data.

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m 3



# Group IV - 20000 mg/m3Page 529Page 530Page 531Page 532Page 533Page 534Page 535Page 536Page 537Page 538Page 539Page 540Page 541Page 542Page 543Page 544Page 545Page 546Page 547Page 548Page 549Page 550Page 551Page 552Page 553Page 554Page 555Page 556Page 557Page 558Page 559Page 560Page 561Page 562Page 563Page 564Page 565Page 566Page 567Page 568Page 569Page 570

# AUDITED FINAL REPORT

Study Phase: Sperm Analysis

Test Site Phase Reference Number 93075134

Te s ting Facility Study Number 00-4206

Sponsor Reference Number 211-TBA-1G



# Gas oline TBA Vapor Condensate:

A One-Generation Whole-Body Inhalation Reproduction

Toxicity Study in Rats

TEST SITE:

Cha rles River Laboratories, Pathology Associates - Maryland

15 Worman’s Mill Court, Suite I

Frederick, MD 21701

TESTING FACILITY:

Huntington Life Sciences (HLS)

Mettlers Road

P.O. Box 2360

East Millstone, NJ 08875-2360

SPONSOR:

American Petroleum Institute (API)

1220 L Street, Northwest

Washington, D.C. 20005-4070

09-APR-2009

Page 1 of 14 Appendix BB Page 571

## Final Report

HLS Study No.: 00-4206

2 

# TABLE OF CONTENTS

Section Page No.

I. QUALITY ASSURANCE STATEMENT .............................. 4

II. MATERIALS AND METHODS .................................... 5

A. Sample Collection................................... 5

B. Sperm Motility Evaluation........................... 5

C. Caudal Epididymis Weight and Total Sperm Count

Determination ...................................... 6

D. Sperm Morphology Evaluation......................... 6

E. Statistical Analyses................................ 6

F. GLP Compliance Statement............................ 7

G. Sample and Data Archiving........................... 7

III. RESULTS .................................................. 7

A. Sperm Motility...................................... 7

B. Total Sperm Count................................... 7

C. Sperm Morphology.................................... 8

D. Caudal Epididymis Weight............................ 8

IV. DISCUSSION AND CONCLUSIONS ............................... 8

V. REFERENCES ............................................... 9 Page 572

## Final Report

HLS Study No.: 00-4206

3 

# INDEX OF TABLES AND APPENDICES

Table Page No.

1. Summary of Male Reproductive Parameters .................10

Appendix Page No.

A. Individual Caudal Epididymis Weight,

Sperm Motility and Sperm Count Data .....................11

B. Individual Sperm Morphology Data .........................13 Page 573Page 574

## Final Report

HLS Study No.: 00-4206

5 

# Gasoline TBA Vapor Condensate:

A One-Generation Whole-Body Inhalation Reproduction Toxicity

Study in Rats

II. MATERIALS AND METHODS

A. Sample Collection

All surviving male rats were euthanized by exsanguination

following anesthesia with inhaled carbon dioxide after the last F 1 

# litters were weaned. For all animals, the abdominal cavity was

opened and the reproductive organs exposed. For motility

assessment, the left vas deferens was dissected away from the

testis and immediately placed in a petri dish containing 10 ml of

a solution consisting of 1% Bovine Serum Albumin dissolved in

Phosphate Buffered Saline. The solution was prewarmed to a

temperature of approximately 38 o 

# C. A minimum 3-minute period was

allowed for the sperm to swim out.

For total sperm count assessment, the left epididymis and

left testis were then removed and placed on dry ice. The frozen

epididymides and testes were then transferred to Pathology

Associates, A Division of Charles River Laboratories, Inc.

(formerly known as Pathology Associates International until

January 8, 2001), Frederick, MD. and stored frozen at -70 o 

# C until

evaluation for caudal epididymal sperm count and homogenizationresistant testicular sperm count.

Only the control and high-exposure animals were examined for

percent motility, total epididymal sperm, homogenization-resistant

testicular spermatid count, sperm morphology and caudal epididymis

weight.

B. Sperm Motility Evaluation

Following the swim out period, a sperm sample was obtained

using a 100 μ m deep cannula. The cannula was immediately loaded

into the prewarmed stage of the Hamilton Thorne IVOS (Ver. 12.1c)

automated sperm analyzer. The analyzer automatically selected

five fields and each motion image was digitally saved and

permanently stored on optical media. The images were

subsequently analyzed and the percent motility determined for

each animal. Page 575

## Final Report

HLS Study No.: 00-4206

6 

# C. Caudal Epididymis Weight and Total Sperm Count Determination

Each frozen epididymis was removed from the freezer, thawed

and the caudal section was trimmed and weighed. Each frozen

testis was removed from the freezer, thawed, the tunica removed

and the testicular parenchyma weighed. The cauda epididymis and

testicular parenchyma were homogenized in deionized water and the

suspensions were transferred to plastic test tubes and vortexed. A

100 μ l sample was transferred to a violet reaction vial containing

a Hoechst dye (H33342) which uniquely stains the head of the

sperm. A sample of the stained sperm was placed into a 20 μ m deep

Cell-Vu glass slide which was loaded into the Hamilton Thorne IVOS

(Ver. 12.1c) analyzer. Twenty fields were automatically selected

by the analyzer for each animal and the number of homogenizationresistant spermatid and cauda epididymal sperm determined. The

counts reported were adjusted for caudal epididymal or testicular

weight.

D. Sperm Morphology Evaluation

Two Eosin stained slides were prepared for each animal from

the caudal epididymis total count preparation. The slides were

evaluated and a minimum of 200 sperm cells/animal was examined for

morphological development.

E. Statistical Analyses

The means and standard deviations for the sperm motility,

caudal epididymal sperm count, testicular homogenization-resistant

spermatid count and sperm morphology data were calculated and

compared across groups using the Kruskal-Wallis nonparametric

ANOVA test. If a significant effect occurred (p<0.05), the MannWhitney U test was used for pair-wise comparisons of each treated

group to the control group. Animals with fewer than 25 sperm

cells present in the motility analysis were excluded from the

group mean calculation and subsequent statistical analysis.

Animals with at least 25 sperm cells were included in the group

mean calculation subsequent and statistical analysis; however,

animals with fewer than 50 cells were identified to have a low

count. The mean caudal epididymis weight for each group was

calculated and compared using one-way analysis of variance (ANOVA)

technique. If ANOVA is significant, Dunnett's test was used for

pair-wise comparisons of each treated group to the control group

at the 5% and 1% risk levels. Statistical analyses were

performed using an IBM TM 

# compatible computer with SAS computer

programs (SAS/STAT User's Guide, 1989). Page 576

## Final Report

HLS Study No.: 00-4206

7 

# F. GLP Compliance Statement

The portion of this study performed by PAI was conducted in

compliance with US EPA 79.60, CFR Vol. 59, No. 122, 27 June 1994.

G. Sample and Data Archiving

Frozen epididymis and testis samples not processed for sperm

count determination were transferred to and stored frozen at -70 o 

# C

in PAI's long-term archive facility. The optical media used for

permanent storage of the sperm motility images, raw data printouts

from the sperm motility, epididymal and testicular sperm count and

sperm morphology analysis, as well as any supporting documentation

and the sperm morphology slides will be maintained at PAI until

the study has been completed. Upon finalizing the report, all

non-frozen study material will be returned to HLS for archiving.

Disposition of the frozen tissue samples will be directed by

correspondence from HLS.

III. RESULTS

A. Sperm Motility

Table 1 (Summary Data)

Appendix A (Individual Data)

No apparent treatment-related effects were observed in the

sperm motility data. Group mean values were 89% and 90% for the

control and high-exposure groups, respectively. There was one

animal (animal 1042) in the control group with no motile sperm.

B. Total Sperm Count

Table 1 (Summary Data)

Appendix A (Individual Data)

The number of caudal epididymal sperm and homogenizationresistant testicular spermatids were comparable between the

control and high-exposure groups. Group mean values were 876.5 and

793.9 million sperm/gram of epididymis and 108.3 million

spermatids/gram of testis for both the control and high-exposure

group. Page 577Page 578

## Final Report

HLS Study No.: 00-4206

9 

# V. REFERENCES

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV

functions for calculating probabilities associated with

Dunnett’s test. Behav. Res. Methods and Instrumentation 13:

363-366.

Dunnett, C. W. 1955. A multiple comparison procedure for

comparing several treatments with a control. Journal of the

American Statistical Association 50:1096-1121.

Dunnett, C. W. 1964. New tables for multiple comparisons with a

control. Biometrics 20:482-491.

Kruskal, W.H. and Wallis, W.A. 1952. Use of ranks in onecriterion variance analysis. Journal of the American

Statistical Association 47: 583-621.

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis

(1952). Journal of the American Statistical Association 48:

907-911.

Kruskal, W.H. 1957. Historical notes on the Wilcoxon unpaired

two-sample test. Journal of the American Statistical

Association 52: 356-360.

SAS/STAT User's Guide, 1989a. SAS Institute Inc., Version 6,

Fourth Edition, Cary, NC:SAS Institute Inc., 1989. Vol. 1,

p. 209-244. Vol. 2 p. 1195-1210. Page 579

### 10 

## HLS STUDY NO.: 00-4206

TABLE 1

GASOLINE TBA VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MALE REPRODUCTIVE PARAMETERS

Group: I IV

Exposure Level: GTBAVC a 

## (mg/m 3 

## ) 0 20,000

MOTILITY (%)

Mean 89 90

SD 6 5

N 25 26

EPIDIDYMAL COUNT

(MILLION SPERM/GRAM)

Mean 876.5 793.9

SD 174.0 115.7

N 26 26

TESTICULAR COUNT

(MILLION SPERM/GRAM)

Mean 108.3 108.3

SD 16.0 13.5

N 26 26

SPERM MORPHOLOGY b



## (% ABNORMAL)

Mean 0.7 0.5

SD 0.6 0.5

N 26 26

CAUDAL EPIDIDYMIS WEIGHTS

(GRAM)

Mean 0.28 0.26 *****



## SD 0.03 0.03

N 26 26

a 

## GASOLINE TBA VAPOR CONDENSATE.

b 

## MEAN AND STANDARD DEVIATIONS WERE CALCULATED USING THE TOTAL NUMBER OF ABNORMAL SPERM

AS A PERCENTAGE OF THE NUMBER OF SPERM EXAMINED.

***** 

## SIGNIFICANTLY DIFFERENT FROM CONTROL GROUP (p<0.05). 

# Page 580

### 11 

## HLS STUDY NO.: 00-4206

APPENDIX A

GASOLINE TBA VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT,

SPERM MOTILITY AND SPERM COUNT DATA

Group I: 0 mg/m 3 

## (GASOLINE TBA VAPOR CONDENSATE)

S P E R M C O U N T

Animal Caudal Motility Caudal Homogenization Resistant

No. Epididymis Epididymal Testicular

Weight (g) (%) (million sperm/gram)

1026 0.25 83 1166.9 80.5

1027 0.26 94 997.4 105.8

1028 0.30 91 920.2 118.5

1029 0.25 86 1422.1 123.9

1030 0.30 90 780.7 128.6

1031 0.30 90 853.9 100.9

1032 0.25 94 723.6 88.8

1033 0.32 92 1032.6 84.4

1034 0.28 91 765.6 93.5

1035 0.25 94 832.3 131.9

1036 0.24 84 1019.5 120.7

1037 0.30 89 913.2 85.7

1038 0.27 90 782.3 104.6

1039 0.20 94 716.3 117.6

1040 0.33 92 817.5 97.1

1041 0.26 88 969.2 135.5

1042 0.28 a 

## 743.1 123.6

1043 0.31 95 731.9 122.0

1044 0.30 91 742.4 111.4

1045 0.29 94 1031.2 117.5

1046 0.30 95 745.9 105.2

1047 0.30 92 979.4 98.5

1048 0.22 89 627.4 124.4

1049 0.28 89 855.2 110.9

1050 0.29 68 948.3 88.0

1051 0.24 78 670.9 97.1

a 

## NO SPERM PRESENT FOR ANALYSIS. 

# Page 581

### 12 

## HLS STUDY NO.: 00-4206

APPENDIX A

GASOLINE TBA VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT,

SPERM MOTILITY AND SPERM COUNT DATA

Group IV: 20,000 mg/m 3 

## (GASOLINE TBA VAPOR CONDENSATE)

S P E R M C O U N T

Animal Caudal Motility Caudal Homogenization Resistant

No. Epididymis Epididymal Testicular

Weight (g) (%) (million sperm/gram)

4026 0.30 94 923.6 128.6

4027 0.23 96 1063.8 137.9

4028 0.23 88 600.1 118.6

4029 0.25 83 824.0 101.6

4030 0.28 89 832.8 82.9

4031 0.22 92 803.2 105.1

4032 0.29 90 757.2 114.3

4033 0.32 92 895.3 101.9

4034 0.26 96 744.0 129.2

4035 0.28 84 956.0 85.3

4037 0.20 84 569.9 109.9

4038 0.23 95 581.9 119.5

4039 0.26 97 852.6 109.4

4040 0.31 94 823.0 101.4

4041 0.23 93 681.9 127.8

4042 0.23 93 768.3 104.6

4043 0.25 93 828.1 112.5

4044 0.28 77 881.3 103.9

4045 0.28 96 911.2 98.3

4046 0.30 95 829.5 85.0

4047 0.28 91 858.9 105.3

4048 0.23 89 722.9 104.6

4049 0.26 92 744.0 106.5

4050 0.25 86 723.6 118.0

4051 0.21 84 687.1 101.5

4052 0.23 86 777.4 103.2 

# Page 582HLS STUDY NO.: 00-4206APPENDIX BGASOLINE TBA VAPOR CONDENSATE:A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL SPERM MORPHOLOGY DATA13Group I: 0 mg/m3(GASOLINE TBA VAPOR CONDENSATE)Animal ----------------H e a d---------------- --------T a i l--------No. NormalAmorphous Small Enlarged Double Coiled Bent Double Other1026 199 1 0 0 0 0 0 0 01027 197 3 0 0 0 0 0 0 01028 199 1 0 0 0 0 0 0 01029 196 4 0 0 0 0 0 0 01030 200 0 0 0 0 0 0 0 01031 199 1 0 0 0 0 0 0 01032 198 1 0 0 0 0 1 0 01033 199 1 0 0 0 0 0 0 01034 200 0 0 0 0 0 0 0 01035 198 1 1 0 0 0 0 0 01036 200 0 0 0 0 0 0 0 01037 198 1 1 0 0 0 0 0 01038 199 1 0 0 0 0 0 0 01039 200 0 0 0 0 0 0 0 01040 200 0 0 0 0 0 0 0 01041 199 1 0 0 0 0 0 0 01042 199 1 0 0 0 0 0 0 01043 200 0 0 0 0 0 0 0 01044 197 2 1 0 0 0 0 0 01045 196 4 0 0 0 0 0 0 01046 199 0 1 0 0 0 0 0 01047 200 0 0 0 0 0 0 0 01048 198 0 0 2 0 0 0 0 01049 200 0 0 0 0 0 0 0 01050 199 0 0 1 0 0 0 0 01051 197 2 1 0 0 0 0 0 0Page 583HLS STUDY NO.: 00-4206APPENDIX BGASOLINE TBA VAPOR CONDENSATE:A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL SPERM MORPHOLOGY DATA14Group IV: 20,000 mg/m3(GASOLINE TBA VAPOR CONDENSATE)Animal ----------------H e a d---------------- --------T a i l--------No. NormalAmorphous Small Enlarged Double Coiled Bent Double Other4026 199 1 0 0 0 0 0 0 04027 197 3 0 0 0 0 0 0 04028 198 1 0 1 0 0 0 0 04029 200 0 0 0 0 0 0 0 04030 199 1 0 0 0 0 0 0 04031 198 1 1 0 0 0 0 0 04032 200 0 0 0 0 0 0 0 04033 197 3 0 0 0 0 0 0 04034 198 2 0 0 0 0 0 0 04035 199 1 0 0 0 0 0 0 04037 200 0 0 0 0 0 0 0 04038 200 0 0 0 0 0 0 0 04039 199 1 0 0 0 0 0 0 04040 200 0 0 0 0 0 0 0 04041 200 0 0 0 0 0 0 0 04042 198 2 0 0 0 0 0 0 04043 200 0 0 0 0 0 0 0 04044 199 0 1 0 0 0 0 0 04045 198 1 1 0 0 0 0 0 04046 199 1 0 0 0 0 0 0 04047 200 0 0 0 0 0 0 0 04048 199 1 0 0 0 0 0 0 04049 198 2 0 0 0 0 0 0 04050 200 0 0 0 0 0 0 0 04051 198 1 1 0 0 0 0 0 04052 200 0 0 0 0 0 0 0 0Page 584

# Huntingdon Life Sciences 00-4206 Page 585

211-TBA-1G Final Report

Certificates of Analysis Appendix CC

1. Feed Certificates of Analysis .....................................................................................586

2. Water Certificates of Analysis ...................................................................................598

3. Bedding Certificates of Analysis ...............................................................................609

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# Huntingdon Life Sciences 00-4206 Page 648

211-TBA-1G Final Report

Historical Control Data

Preface Appendix EE

1. Huntingdon Life Sciences: CD Rat - Mating, Fertility and Gestation

Data used for historical control purposes was obtained from inhalation studies run

prior to this study (from April 2001 to May 2002).

2. Charles River Pathology Associates: Sprague-Dawley Rat Sperm AnalysisHuntingdon Life Sciences00-4206Page 649211-TBA-1GFinal ReportHistorical Control DataCD Rat - Mating, Fertility and GestationAppendix EEFemalesStudy Initiation Paired w/Mated Mating Index PregnantFertilityIndexNo. withLivebornGestationIndexID Date MalesNo. No. % No. % No. %00-4208 P0 12-Jun-01 262492.32395.823100.000-4208 F1262388.52191.321100.000-4207 P0 30-Apr-01 262596.22496.024100.000-4207 F1262596.225100.025100.000-4205 20-May-02 262596.22288.022100.000-4204 19-Mar-02 2626100.02596.225100.000-4203 21-Jan-02 2626100.02596.225100.000-4202 22-Aug-01 2626100.02492.324100.0MEAN25 96.2 24 94.5 24 100SD1.1 4.10 1.5 3.73 1.5 -N8 8 8 8 8 8MIN23 88.5 21 88.0 21 100MAX26 100 25 100 25 100Page 650

# Huntingdon Life Sciences 00-4206 Page 651

211-TBA-1G Final Report

Testing Facility Personnel Appendix FF

TITLE/DEPARTMENT NAME/DEGREE

SENIOR VICE PRESIDENT,

SAFETY ASSESSMENT Sylvie J. Gosselin, D.V.M., Ph.D.,

Diplomate A.C.V.P.

SENIOR DIRECTOR, SAFETY

ASSESSMENT Carol S. Auletta, M.B.A., D.A.B.T.,

R.A.C.

DIRECTOR, ANALYTICAL SERVICES Barbara A. Litzenberger, B.S.,

M.T. (ASCP)

DIRECTOR, TOXICOLOGY

OPERATIONS Ian Vanterpool, F.I.A.T.

DIRECTOR, QUALITY ASSURANCE Nicki S. Iacono, B.S.

STUDY DIRECTOR Gary M. Hoffman, B.A., D.A.B.T.

PATHOLOGIST Katharine M. Whitney, D.V.M.,

Ph.D., Diplomate A.C.V.P.

STUDY MONITOR Janet E. Scimone, B.A.

VETERINARIAN Teresa S. Kusznir, V.M.D.

MANAGER/SUPERVISOR

Reproductive and Inhalation Toxicology

Analytical Services

Histology Robert Faust, B.S.

Kay Saladdin, B.S.

Janet Kusisto

# Huntingdon Life Sciences 00-4206 Page 652

211-TBA-1G Final Report

Report Amendments Appendix GG

There are no amendments for this report at this time.