PROGRESS REPORT: SEPTEMBER 2010 

BIODEGRADATION TESTING SUPPLEMENTAL ENVIRONMENTAL PROJECT 

Submitted to 

E.I du Pont de Nemours and Company 

Wilmington, Delaware 19898 

U.S.A. 

United States Environmental Protection Agency 

Washington, DC 

U.S.A. 

Wildlife International, Ltd.

8598 Commerce Drive 

Easton, Maryland 21601 USA 

1 (410) 822-8600 

September 29, 2010 

Page 1 of 5 
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2010_September_Progress Report 2

Table of Contents 

Title Page ...................................................................................................................................1 

Table of Contents.......................................................................................................................2 

Introduction................................................................................................................................3 

Progress Summary .....................................................................................................................3 

Appendix A Invoice...................................................................................................................4 
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2010_September_Progress Report 3

Introduction 

This document presents the progress of the technical work for the Biodegradation Testing 

Supplemental Environmental Project (“Biodegradation SEP”) performed from 25 August 

2010 up to 24 September 2010. Studies conducted under protocol will be examined by the 

Wildlife International Quality Assurance Unit for compliance with Good Laboratory 

Practices, Standard Operating Procedures and the specified protocol. Raw data that has been 

audited by the Wildlife International Quality Assurance will be included in the progress 

report, as appropriate. 

Progress Summary 

The total costs associated with the project for the period covered in this Progress Report are 

approximately $116,578 for labor and $18,900 for instrumentation. The invoice and an 

allocation of costs with associated tasks are presented in Appendix A. 

The technical work consisted of analytical processing of samples from the initial pilot study. 

Bench processing and determination of study analytes by GC/MS and LC/MS/MS has been 

completed for all pilot study samples. 

Estimated completion dates for un-audited and audited draft report are 22 October and 17 

November, respectively. 
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2010_September_Progress Report 4

Appendix A 

Invoice 
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2010_September_Progress Report 

Allocation of Costs 

Month, Year Tasks 

 Design and 

Procurement of 

SCAS Units 

(Task A) 

Acquisition 

of Standards 

(Task B) 

Adaptation of 

Analytical 

Method (Task C) 

Pilot Studies 

Task (D) 

Refinement of 

Methods 

Task (E) 

Method 

Validation

s (Task F) 

Storage 

Stability 

(Task G) 

Biod

dat

Stu

(Tas

Dec 2009 $1,803 $1,085 $42,422 Jan 2010 $29,227 Feb 2010 $1,300 $36,745 Mar 2010 $14,350 Apr 2010 $570 $15,935 May 2010 $760 $71,860 June 2010 $57,870 $35,003 July 2010 $42,430 $15,130 August 2010 $32,530 $113,718 September 2010 $1,350 $115,228 Labor Total $1,803 $3,715 $344,719 $279,078 Materials $6,800 $10,500 $20,644 $1,724 Total Cost $8,603 $14,215 $365,363 $280,802