### Results Table Extracted from SCO Lymphocyte Chromosomal Aberration Study

Study Title



# EVALUATION OF THE AB ILITY OF CASTOR OIL, SULFATED,

SODIUM SALT, 75% TO INDUCE CHROMOSOME AB ERRATIONS

IN CULTURED PERIPHER AL HUMAN LYMPHOCYTES

(WITH REPEAT EXPERIM ENT)

Author

C.A.F. Buskens, MSc.



### Tables 6, 7 and 8

### Table 1 Chromosome aberrations in human lymphocyte cultures treated with Castor Oil,

Sulfated, Sodium Salt, 75% in the absence of S9

- mix in the second cytogenetic

assay (24 h exposure time, 24 h fixation time)

Conc Ethanol

(0.5% v/v) 10

μ g/ml 200

μ g/ml 300

μ g/ml MMC

- C

0.2 μ g/ml

Culture A B A+B A B A+B A B A+B A B A+B A B A+B

Mitotic

Index (%) 100 100 89 41 65

No. of

Cells

scored 100 100 200 100 100 200 100 100 200 100 100 200 100 100 200

No. of

Cells with

aberrations

(+ gaps) a) 2 0 2 1 1 2 0 1 1 1 0 1 13 11 *** )

24

No. of

Cells with

aberrations

(

- gaps) 1 0 1 1 0 1 0 1 1 1 0 1 11 8 *** )

19

g’ 1 1 2

g” 2 3

b’ 1 1 4 4

b” 1 6 4

m’ 1 1

m” 7 1

exch. 1

dic

d’

misc. poly poly poly

total aberr

(+ gaps) 2 0 1 1 0 1 7 0 15 14

total aberr

(

- gaps) 1 0 1 0 0 1 7 0 13 9

a) Abbreviations used for various types of aberrations (see listing in robust endpoint summary)

. misc. = (miscellaneous) aberrations not belonging to the ones mentioned above.

The numerical variation polyploidy (p oly) was not counted as an aberration.

*) Significantly different from control group (Chi

- square test), * P < 0.05, ** P < 0.01 or *** P < 0.001.

### Table 2 Chromosome aberrations in human lymphocyte cultures treated with Castor Oil,

Sulfated, Sodium Salt, 75% in the absence of S9

- mix in the second cytogenetic

assay (48 h exposure time, 48 h fixation time)

Conc Ethanol

(0.5% v/v) 10

μ g/ml 100

μ g/ml 200

μ g/ml MMC

- C

0.1 μ g/ml

Culture A B A+B A B A+B A B A+B A B A+B A B A+B

Mitotic

Index (%) 100 83 70 54 66

No. of

Cells

scored 100 100 200 100 100 200 100 100 200 100 100 200 100 100 200

No. of

Cells with

aberrations

(+ gaps) a) 0 2 2 0 2 2 0 1 1 0 2 2 8 18 *** )

26

No. of

Cells with

aberrations

(

- gaps) 0 1 1 0 0 0 0 1 1 0 1 1 8 15 *** )

23

g’ 2

g” 1 3 1 2 2

b’ 2 7

b” 1 1 1 5 6

m’

m” 2

exch.

dic

d’

misc. p 3p

total aberr

(+ gaps) 0 2 0 3 0 1 0 2 10 22

total aberr

(

- gaps) 0 1 0 0 0 1 0 1 8 18

a) Abbreviations used for various types of aberrations (see endpoint robust summary)

mis c. = (miscellaneous) aberrations not belonging to the ones mentioned above.

*) Significantly different from control group (Ch i

- square test), * P < 0.05, ** P < 0.01 or *** P < 0.001.

### Table 3 Chromosome aberrations in human lymphocyte cultures treated with Castor Oil,

Sulfated, Sodium Salt, 75% in the presence of S9

- mix in the second cytogenetic

assay (3 h exposure time, 48 h fixation time)

Conc Ethanol

(0.5% v/v) 100

μ g/ml 300

μ g/ml 500

μ g/ml CP

10 μ g/ml

Culture A B A+B A B A+B A B A+B A B A+B A B A+B

Mitotic

Index (%) 100 66 57 68

- b)

No. of

Cells

scored 100 100 200 100 100 200 100 100 200 100 100 200 100 100 200

No. of

Cells with

aberrations

(+ gaps) a) 0 0 0 0 0 0 0 1 1 1 0 1 28 25 *** )

53

No. of

Cells with

aberrations

(

- gaps) 0 0 0 0 0 0 0 0 0 1 0 1 24 22 *** )

46

g’ 1 5 2

g” 3

b’ 1 15 12

b” 9 9

m’ 2

m” 2

exch. 3

dic

d’

misc.

total aberr

(+ gaps) 0 0 0 0 0 1 1 0 31 31

total aberr

(

- gaps) 0 0 0 0 0 0 1 0 26 26

a) Abbreviations used for various types of aberrations (see endpoint robust summary)

misc. = (miscellaneous) aberrations not belonging to the ones mentioned above.

b) CP was fixed after 24 hours. Therefore, the mi totic index could not be calculated as percentage of control.

*) Significantly different from control group (Chi

- square test), * P < 0.05, ** P < 0.01 or *** P < 0.001.