Results Table Extracted from SCO Lymphocyte Chromosomal Aberration Study

Study Title

EVALUATION OF THE ABILITY OF CASTOR OIL, SULFATED, 

SODIUM SALT, 75% TO INDUCE CHROMOSOME ABERRATIONS 

IN CULTURED PERIPHERAL HUMAN LYMPHOCYTES

(WITH REPEAT EXPERIMENT)

Author

C.A.F. Buskens, MSc.

Tables 6, 7 and 8
Table 1 Chromosome aberrations in human lymphocyte cultures treated with Castor Oil, 

Sulfated, Sodium Salt, 75% in the absence of S9-mix in the second cytogenetic 

assay (24 h exposure time, 24 h fixation time)

Conc Ethanol

(0.5% v/v)

10

μg/ml

200

μg/ml

300

μg/ml

MMC-C

0.2 μg/ml

Culture A B A+B A B A+B A B A+B A B A+B A B A+B

Mitotic

Index (%) 100 100 89 41 65

No. of

Cells 

scored

100 100 200 100 100 200 100 100 200 100 100 200 100 100 200

No. of

Cells with

aberrations

(+ gaps) a)

2 0 2 1 1 2 0 1 1 1 0 1 13 11

***)

24

No. of

Cells with

aberrations

(- gaps)

1 0 1 1 0 1 0 1 1 1 0 1 11 8

***)

19

g’ 1 1 2

g” 2 3

b’ 1 1 4 4

b” 1 6 4

m’ 1 1

m” 7 1

exch. 1

dic

d’

misc. poly poly poly

total aberr

(+ gaps) 2 0 1 1 0 1 7 0 15 14

total aberr

(- gaps) 1 0 1 0 0 1 7 0 13 9

a)

Abbreviations used for various types of aberrations (see listing in robust endpoint summary).

misc. = (miscellaneous) aberrations not belonging to the ones mentioned above.

The numerical variation polyploidy (poly) was not counted as an aberration.

*) Significantly different from control group (Chi-square test), * P < 0.05, ** P < 0.01 or *** P < 0.001.
Table 2 Chromosome aberrations in human lymphocyte cultures treated with Castor Oil, 

Sulfated, Sodium Salt, 75% in the absence of S9-mix in the second cytogenetic 

assay (48 h exposure time, 48 h fixation time)

Conc Ethanol

(0.5% v/v)

10

μg/ml

100

μg/ml

200

μg/ml

MMC-C

0.1 μg/ml

Culture A B A+B A B A+B A B A+B A B A+B A B A+B

Mitotic

Index (%) 100 83 70 54 66

No. of

Cells 

scored

100 100 200 100 100 200 100 100 200 100 100 200 100 100 200

No. of

Cells with

aberrations

(+ gaps) a)

0 2 2 0 2 2 0 1 1 0 2 2 8 18

***)

26

No. of

Cells with

aberrations

(- gaps)

0 1 1 0 0 0 0 1 1 0 1 1 8 15

***)

23

g’ 2

g” 1 3 1 2 2

b’ 2 7

b” 1 1 1 5 6

m’

m” 2

exch.

dic

d’

misc. p 3p

total aberr

(+ gaps) 0 2 0 3 0 1 0 2 10 22

total aberr

(- gaps) 0 1 0 0 0 1 0 1 8 18

a)

Abbreviations used for various types of aberrations (see endpoint robust summary)

misc. = (miscellaneous) aberrations not belonging to the ones mentioned above.

*) Significantly different from control group (Chi-square test), * P < 0.05, ** P < 0.01 or *** P < 0.001.
Table 3 Chromosome aberrations in human lymphocyte cultures treated with Castor Oil, 

Sulfated, Sodium Salt, 75% in the presence of S9-mix in the second cytogenetic 

assay (3 h exposure time, 48 h fixation time)

Conc Ethanol

(0.5% v/v)

100

μg/ml

300

μg/ml

500

μg/ml

CP

10 μg/ml

Culture A B A+B A B A+B A B A+B A B A+B A B A+B

Mitotic

Index (%) 100 66 57 68 -

b)

No. of

Cells 

scored

100 100 200 100 100 200 100 100 200 100 100 200 100 100 200

No. of

Cells with

aberrations

(+ gaps) a)

0 0 0 0 0 0 0 1 1 1 0 1 28 25

***)

53

No. of

Cells with

aberrations

(- gaps)

0 0 0 0 0 0 0 0 0 1 0 1 24 22

***)

46

g’ 1 5 2

g” 3

b’ 1 15 12

b” 9 9

m’ 2

m” 2

exch. 3

dic

d’

misc.

total aberr

(+ gaps) 0 0 0 0 0 1 1 0 31 31

total aberr

(- gaps) 0 0 0 0 0 0 1 0 26 26

a)

Abbreviations used for various types of aberrations (see endpoint robust summary)

misc. = (miscellaneous) aberrations not belonging to the ones mentioned above.

b) CP was fixed after 24 hours. Therefore, the mitotic index could not be calculated as percentage of control.

*) Significantly different from control group (Chi-square test), * P < 0.05, ** P < 0.01 or *** P < 0.001.