Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON, D.C. 20460 

OFFICE OF CHEMICAL SAFETY 

AND POLLUTION PREVENTION 

MEMORANDUM 

Date: 25 January 2013 

SUBJECT: Cyantraniliprole. Petition for the Establishment of Permanent Tolerances and 

Registration for Use on Brassica (cole) Leafy Vegetables (Group 5), Bulb 

Vegetables (Group 3-07), Bushberries (Group 13-07B), Oilseed (Group 20), 

Citrus Fruit (Group 10-10), Cucurbit Vegetables (Group 9), Fruiting Vegetables 

(Group 8-10), Leafy Vegetables (non-Brassica) (Group 4), Pome Fruit (Group 

11-10), Stone Fruit (Group 12), Tree Nuts (Group 14), Tuberous and Corm 

Vegetables (Subgroup 1C), and Food/Feed Handling Establishments. Summary 

of Analytical Chemistry and Residue Data. 

PC Code: 090098 DP Barcodes: D407961 

Decision No.: 451670 Registration Nos.: 352-ILA, 352-ILT, 352-ILO, 

352-IAN, 352-ILI, 352-IA T 

Petition No.: IF7894 Regulatory Action: Section 3 Registration 

Risk Assessment Type: NA CaseNo.: NA 

TXRNo.: NA CAS No.: 736994-63-1 

MRID No.: See MRID Summary Table 40 CFR: To be detennined 

FROM: Steve Funk, Senior Scientist ~ ~ l _/ 

Risk Assessment Branch III ¥....-

Health Effects Division (7509P 

THROUGH: Jeff Dawson, Acting Branch Chief 

Risk Assessment Branch III 

Health Effects Division (7509P) 

TO: 

And 

Thurston Morton, Senior Chemist~· sk Assessment Branch IV 

Donna 

Donald 

Davis, 

Wilbur, 

Co-Chair 

Co-Chair 

~ , ~ 

r~ 

Chemistry Science Advisory oun I hemSAC) 

Health Effects Division (7509P) 

John Hebert, RM07 

Thomas Harris 

Insecticide Branch 

Registration Division (7505P) 

Page 1 of196 

Ver.Apr.08 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961 

Summary of Submitted Residue Chemistry Studies (DuPont) 

OCSPP 860 MRIDNumber Monograph Title 

Series AnnexB 

Guideline Reference 

860.1300 48 119988 B.7.1.3 The Metabolism of [Carbon 14)CYANTRANILIPROLE in Cotton 

860.1300 48 119989 B.7.l.4 The Metabolism of [Carbon 14)CYANTRANILIPROLE in 

Tomatoes 

860.1300 48119990 B.7.l.2 The Metabolism of [Carbon 14)CYANTRANILIPROLE in Lettuce 

860.1300 48119991 B.7.2.2 Metabolism of[Carbon 14) CYANTRANILIPROLE in the 

Lactating Goat 

860.1300 48119992 B.7.2.1 The Metabolism of[Carbon 14] CYANTRANILIPROLE in the 

Laying Hen 

860.1340 48119922 8.5.2.1.2 Analytical Method for the Detennination of 

CY ANTRANILIPROLE and Metabolites in Crops Using 

LC/MS/MS 

860.1340 48 119923 B.5.2.l Analytical Method for the Detennination of 

CY ANTRANILIPROLE and Metabolites in Processed Fractions 

Using LC/MS/MS 

860.1340 48119924 B.5.2.l.2 Analytical Method for the Detennination of 

CY ANTRANILIPROLE and Metabolites in Bovine Tissues, Dairy, 

and Eggs Using LC/MS/MS 

860.1340 48119925 8.5.2.1.2 Method Validation for the Analysis of CY ANTRANILIPROLE and 

its Metabolites (IN-N7B69, IN-JCZ38, IN-K7H19, IN-MYX98, INMLA84 and IN-J9Z38) in Various Crops with LC-MS/MS 

Detection 

860.1340 48119928 8.5.2.1.1 Method Assessment and Validation for the Determination of 

Residues of CY ANTRANILIPROLE in Plants Using Multi-Residue 

Method DFG S19 (LC-MS/MS Module) 

860.1340 48119929 8.5.2.1.3 Multiresidue Method Testing for CY ANTRANILIPROLE and Six 

Metabolites According to the FDA Pesticide Analytical Manual 

Volume I (PAM, Vol. I as Revised in October 1999) 

860.1340 48119932 8.5.2.2.2 Validation of an Analytical Method for the Detennination of 

CYANTRANILIPROLE, IN-HGW87, IN-N7B69, IN-K7H19, INJCZ38, IN-MYX98, IN-J9Z38 and IN-MLA84 in Bovine Tissues, 

Milk and Eggs 

860.1340 48119933 8.5.2.2.1 Method Assessment and Validation for the Determination of 

Residues of CY ANTRANILIPROLE and Metabolites in Animal 

Tissues Using Multi-Residue Method DFG S 19 (LC/MS/MS 

Module) 

860.1340 48119934 8.5.2.1.2 Independent Laboratory Validation of Analytical Method for the 

Detennination of CY ANTRANILIPROLE and Metabolites in Crops 

and Processed Fractions Using LC/MS/MS 

860.1340 48119998 8.5.2.1.2 Extraction Efficiency of(Carbon 14]CYANTRANILIPROLE from 

Crops 

860.1340 48119999 8 .5.2.2.1 Independent Laboratory Validation for the Detennination of 

Residues of CY ANTRANILIPROLE in Animal Tissues Using 

Multi-Residue Method DFG S 19 (LC-MS/MS Module) 

860. 1340 48120001 8.5.2.2.2 Extraction Efficiency of (Carbon 14 ]CY ANTRANILIPROLE from 

Muscle, Liver and Milk from Goats and Eggs from Hens 

860.1340 48122526 B.5.2.1 Independent Laboratory Validation for the Detennination of 

Residues of CY ANTRANILIPROLE in Plants using Multi-Residue 

Method DFG S 19 (LC-MS/MS Module) 

860. 1340 48 122534 8.5.2.2 Independent Laboratory Validation of DuPont-18844 Method for the 

Detennination ofResidues ofCYANTRANILIPROLE and 

Metabolites in Milk and Bovine Tissues Using LC/MS/MS 

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Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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860.1380 48119993 B.7.6.2 Stability of CYANTRANILIPROLE and Metabolite/Degradation 

Product Residues in Representative Crops Stored Frozen

860.1380 48120014 B.7.8.2 CYANTRANILIPROLE: Magnitude of Residues of 

Cyantraniliprole (CYANTRANILIPROLE) and Metabolites in 

Edible Tissues and Milk of Lactating Diary Cows following Dosing 

with Cyantraniliprole

860.1380 48120015 B.7.8.1 Magnitude of Residues of Cyantraniliprole 

(CYANTRANILIPROLE) and Metabolites in Edible Tissues and 

Eggs of Poultry following Dosing with Cyantraniliprole

860.1480 48120014 B.7.8.2 CYANTRANILIPROLE: Magnitude of Residues of 

Cyantraniliprole (CYANTRANILIPROLE) and Metabolites in 

Edible Tissues and Milk of Lactating Diary Cows following Dosing 

with Cyantraniliprole

860.1480 48120015 B.7.8.1 Magnitude of Residues of Cyantraniliprole 

(CYANTRANILIPROLE) and Metabolites in Edible Tissues and 

Eggs of Poultry following Dosing with Cyantraniliprole

860.1500 48120007 B.7.6.1.5

(Europe)

Magnitude and Decline of CYANTRANILIPROLE and Metabolite 

Residues in Protected Peppers (Fruiting Vegetables, Soanacea) 

following Foliar Application of CYANTRANILIPROLE 100 g/L 

OD or Drip Irrigation Application of CYANTRANILIPROLE 200 

g/L SC – Europe 2008

860.1500 48120008 B.7.1.4 Magnitude and Decline of CYANTRANILIPROLE and Metabolite 

Residues in Brassica Vegetables following Foliar Applications of 

CYANTRANILIPROLE 100 g/L OD, CYANTRANILIPROLE 100 

g/L SE, and Soil Applications of CYANTRANILIPROLE 200 g/L 

SC

860.1500 48120009 B.7.1.6 Magnitude and Decline of CYANTRANILIPROLE and Metabolite 

Residues in Cucurbits (Cucumber, Cantaloupe/Muskmelon, Summer 

Squash) following Foliar Applications of CYANTRANILIPROLE

100 g/L OD, and Soil Applications of CYANTRANILIPROLE 200 

g/L SC

860.1500 48120010 B.7.1.5 Magnitude and Decline of CYANTRANILIPROLE and Metabolite 

Residues in Fruiting Vegetables (Tomato, Bell Pepper, Non-Bell 

Pepper) following Foliar Applications of CYANTRANILIPROLE

100 g/L OD, and Soil Applications of CYANTRANILIPROLE 200 

g/L SC

860.1500 48120011 B.7.1.3 Combined Processing and Magnitude and Decline of 

CYANTRANILIPROLE and Metabolite Residues in Leafy 

Vegetables following Foliar 

Applications of CYANTRANILIPROLE 100 g/L OD, 

CYANTRANILIPROLE 100 g/L SE and Soil Applications of 

CYANTRANILIPROLE 200 g/L SC

860.1500 48120016 B.7.1.2 Magnitude of the Residue on Dry Bulb and Green Onion

860.1500 48120017 B.7.1.9 Combined Processing and Magnitude and Decline of 

Cyantraniliprole and Metabolite Residues in Stone Fruit (Peach, 

Plum, and Cherry) following Foliar Applications of 

CYANTRANILIPROLE 100 g/L SE – NAFTA 2009

860.1500 48120018 B.7.1.8 Combined Processing and Magnitude and Decline of 

Cyantraniliprole and Metabolite Residues in Pome Fruit (Apple and 

Pear) following Foliar Applications of CYANTRANILIPROLE 100 

g/L SE – NAFTA 2009

860.1500 48120019 B.7.1.11 Magnitude of Cyantraniliprole and Metabolite Residues in Tree Nuts 

(Almonds and Pecans) following Foliar Applications (DPX-W86 

100 g/L OD and DPX-W86 100 g/L SE) and Soil Applications 

(DPX-W86 200 g/LSC)- NAFTA, 2009

860.1500 48120020 B.7.1.1 Combined Processing and Magnitude of Cyantraniliprole and 

Metabolite Residues in Citrus Fruit (Orange, Lemon, and 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Grapefruit) Following Foliar Applications of 

CYANTRANILIPROLE 100 g/L SE and Systemic Delivery 

Applications of CYANTRANILIPROLE 200 g/L SC.

860.1500 48120021 B.7.1.11 Combined Processing and Magnitude of Cyantraniliprole and 

Metabolite Residues in Cotton following Foliar Applications of 

CYANTRANILIPROLE 100 g/L OD and Soil Applications of 

CYANTRANILIPROLE 200 g/L SC- USA 2009

860.1500 48120022 B.7.1.12 Combined Processing and Magnitude of Cyantraniliprole and 

Metabolite Residues in Oil Seeds (Canola and Sunflowers) 

following Foliar Applications of CYANTRANILIPROLE 100 g/L 

OD and Seed Treatment with 625 g/L FS- NAFTA 2009

860.1500 48120023 B.7.1.4 Combined Processing and Magnitude of Cyantraniliprole and 

Metabolite Residues in Tuberous and Corm Vegetables following 

Foliar Applications (HGW86 100 g/L OD and 100 g/L SE), Soil 

Applications (200 g/L SC),and Seed Treatment (625 g/L FS)-

NAFTA 2009

860.1500 48120034 B.7.1.10 Magnitude of Cyantraniliprole and Metabolite Residues in 

Blueberries following Foliar Applications of 

CYANTRANILIPROLE 100 g/L SE- USA, 2009

860.1500 48120037 B.7.6.1.5

(Europe)

Magnitude and Decline of Cyantraniliprole and Metabolite Residues 

in Protected Tomatoes and Cherry Tomatoes following Foliar 

Application of CYANTRANILIPROLE 100 g/L OD and Soil 

Application of DAPX-W86 200 g/L SC- Europe, 2009

860.1500 48120038 B.7.6.1.5

(Europe)

Magnitude and Decline of Cyantraniliprole and Metabolite Residues 

in Protected Peppers and Hot Peppers following Foliar Application 

of CYANTRANILIPROLE 100 g/L OD and Soil Application of 

DAPX-W86 200 g/L SC- Europe, 2009

860.1520 48120044 B.7.7.1 High Temperature Hydrolysis of [Carbon 14]-

CYANTRANILIPROLE in Buffered Aqueous Solution at pH 4, 5, 

and 6

860.1520 48120011 B.7.1.5

(NAFTA)

Combined Processing and Magnitude and Decline of 

CYANTRANILIPROLE and Metabolite Residues in Leafy 

Vegetables following Foliar 

Applications of CYANTRANILIPROLE 100 g/L OD, 

CYANTRANILIPROLE 100 g/L SE and Soil Applications of 

CYANTRANILIPROLE 200 g/L SC

860.1520 48120012 B.7.1.6

(NAFTA)

Magnitude of CYANTRANILIPROLE and Metabolite Residues in 

Processed Fractions of Tomatoes following Foliar Applications of 

CYANTRANILIPROLE 100 g/L OD – U.S. 2008

860.1520 48120017 B.7.1.11

(NAFTA)

Combined Processing and Magnitude and Decline of 

Cyantraniliprole and Metabolite Residues in Stone Fruit (Peach, 

Plum, and Cherry) following Foliar Applications of 

CYANTRANILIPROLE 100 g/L SE – NAFTA 2009

860.1520 48120018 B.7.1.10

(NAFTA)

Combined Processing and Magnitude and Decline of 

Cyantraniliprole and Metabolite Residues in Pome Fruit (Apple and 

Pear) following Foliar Applications of CYANTRANILIPROLE 100 

g/L SE – NAFTA 2009

860.1520 48120020 B.7.1.8

(NAFTA

Combined Processing and Magnitude of Cyantraniliprole and 

Metabolite Residues in Citrus Fruit (Orange, Lemon, and 

Grapefruit) Following Foliar Applications of 

CYANTRANILIPROLE 100 g/L SE and Systemic Delivery 

Applications of CYANTRANILIPROLE 200 g/L SC.

860.1520 48120021 B.7.1.12

(NAFTA)

Combined Processing and Magnitude of Cyantraniliprole and 

Metabolite Residues in Cotton following Foliar Applications of 

CYANTRANILIPROLE 100 g/L OD and Soil Applications of 

CYANTRANILIPROLE 200 g/L SC- USA 2009

860.1520 48120023 B.7.1.4 Combined Processing and Magnitude of Cyantraniliprole and 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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(NAFTA) Metabolite Residues in Tuberous and Corm Vegetables following 

Foliar Applications (HGW86 100 g/L OD and 100 g/L SE), Soil 

Applications (200 g/L SC),and Seed Treatment (625 g/L FS)-

NAFTA 2009

860.1850 48119986 B.7.9.2 CYANTRANILIPROLE Pilot Confined Crop Rotation Study 

(Wheat, Soybeans, and Red Beets)

860.1850 48119987 B.7.9.2 Confined Rotational Crop Study Using [Carbon 14] 

CYANTRANILIPROLE

860.1900 48119995 B.7.9.3.2 Field Crop Rotation Study with CYANTRANILIPROLE 10OD 

Insecticide - EPA Region 10, USA, 2006

860.1900 48119996 B.7.9.3.1 Field Crop Rotation Study with CYANTRANILIPROLE 10OD 

Insecticide - EPA Region 11, USA, 2006

860.1900 48120005 B.7.9.3.3 Magnitude of CYANTRANILIPROLE and Metabolite and/or 

Degradation Product Residues in Field Rotational Crops following 

Application of CYANTRANILIPROLE 100 g/L OD Insecticide -

Ontario, Canada Location 2008/2009

860.1900 48120035 B.7.9.3.4 Magnitude of Cyantraniliprole and Metabolite Residues in Field 

Rotational Crops following Bare Ground Applications of DPX-100 

G/L SE - USA, 2009

860.1900 48519713 B.7.9.3.5 Magnitude of Cyantraniliprole and Metabolite Residues in Field 

Rotational Crops following Bare Ground Applications of DPX-100 

g/L SE - USA and Canada, 2010

Executive Summary

Cyantraniliprole is a second-generation, systemic insecticide belonging to the diamide class of 

chemistry, mobile in both the xylem and phloem. The mode of action is through its binding with 

insect ryanodine receptors. This causes muscle contraction, which leads to paralysis and eventual 

death of the insect. It is structurally identical to chlorantraniliprole except with the substitution of 

the chlorine atom with a cyano group.

This is the first registration and tolerance request for cyantranilirprole under a global joint review 

(Australia, Canada, France, United Kingdom, USA).

DuPont has proposed tolerances for residues of the insecticide cyantraniliprole, including its 

metabolites and degradates, in or on the commodities below. DuPont has stated that compliance 

with the tolerance levels specified below is to be determined by measuring cyantraniliprole, 3-

bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1Hpyrazole-5-carboxamide in or on the following commodities:

Almond, hulls..........................................................................................................................30 ppm

Berries and small fruits, bushberries (crop subgroup 13-07B)................................................. 4 ppm

Brassica (cole) leafy vegetables, head and stem Brassica (crop subgroup 5A)....................... 2 ppm

Brassica (cole) leafy vegetables, leafy Brassica greens (crop subgroup 5B)..........................30 ppm

Bulb vegetables, onion, bulb (crop subgroup 3-07A)...........................................................0.04 ppm

Bulb vegetables, onion, green (crop subgroup 3-07B)............................................................. 8 ppm

Cattle, fat..............................................................................................................................0.01 ppm

Cattle, liver...........................................................................................................................0.04 ppm

Cattle, meat ..........................................................................................................................0.01 ppm

Cattle, meat byproducts, except liver ...................................................................................0.01 ppm

Cherries.................................................................................................................................... 6 ppm
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Citrus fruits (crop group 10-10) .............................................................................................0.7 ppm

Cotton, gin byproduct..............................................................................................................10 ppm

Cucurbit vegetables (crop group 9) ........................................................................................0.3 ppm

Fruiting vegetables (crop group 8-10)...................................................................................... 2 ppm

Goat, fat................................................................................................................................0.01 ppm

Goat, liver.............................................................................................................................0.04 ppm

Goat, meat ............................................................................................................................0.01 ppm

Goat, meat byproducts, except liver.....................................................................................0.01 ppm

Hog, fat.................................................................................................................................0.01 ppm

Hog, liver..............................................................................................................................0.04 ppm

Hog, meat .............................................................................................................................0.01 ppm

Hog, meat byproducts, except liver......................................................................................0.01 ppm

Horse, fat..............................................................................................................................0.01 ppm

Horse, liver...........................................................................................................................0.04 ppm

Horse, meat ..........................................................................................................................0.01 ppm

Horse, meat byproducts, except liver ...................................................................................0.01 ppm

Leafy vegetables (except Brassica vegetables) (crop group 4) ...............................................15 ppm

Milk......................................................................................................................................0.01 ppm

Milk fat.................................................................................................................................0.04 ppm

Oilseeds, except cotton byproduct (crop group 20).................................................................. 1 ppm

Pome fruits (crop group 11-10)..............................................................................................0.8 ppm

Root and tuber vegetables, tuberous and corm vegetables (crop subgroup 1C)...................0.15 ppm

Sheep, fat..............................................................................................................................0.01 ppm

Sheep, liver...........................................................................................................................0.04 ppm

Sheep, meat ..........................................................................................................................0.01 ppm

Sheep, meat byproducts, except liver...................................................................................0.01 ppm

Stone fruits, except cherries (crop group 12) .........................................................................1.5 ppm

Tree nuts, except almond hulls (crop group 14)...................................................................0.06 ppm

Also, the registrant has proposed tolerances for residues of the insecticide cyantraniliprole, 

including its metabolites and degradates, in or on the [processed] commodities below. DuPont 

has stated that compliance with the tolerance levels specified below is to be determined by 

measuring the sum of cyantraniliprole, 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl6-[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide and its metabolite, IN-J9Z38, 2-

[3-bromo-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-yl]-3,4-dihydro-3,8-dimethyl-4-oxo-6-

quinazolinecarbonitrile, in or on the following commodities, when present therein as a result of 

the application of the pesticide to growing crops listed as follows:

Citrus, oil.................................................................................................................................. 6 ppm

Citrus, raw peel ........................................................................................................................ 2 ppm

Potato, wet peel ......................................................................................................................0.3 ppm

Furthermore, the registrant has proposed tolerances for indirect or inadvertent residues of the 

insecticide cyantraniliprole, including its metabolites and degradates, in or on the commodities 

below. DuPont has stated that compliance with the tolerance levels specified below is to be 

determined by measuring cyantraniliprole, 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-

methyl-6-[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide, in or on the following 

commodities, when present therein as a result of the application of the pesticide to growing crops 

listed as follows:

Foliage of legume vegetables (crop group 7), forage ...........................................................0.15 ppm

Foliage of legume vegetables (crop group 7), hay .................................................................0.6 ppm

Forage, fodder and straw of cereal grains (crop group 16)), forage .....................................0.06 ppm

Forage, fodder and straw of cereal grains (crop group 16)), hay and straw .........................0.15 ppm
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Grass forage, fodder, and hay (crop group 17), forage.........................................................0.06 ppm

Grass forage, fodder, and hay (crop group 17), hay .............................................................0.15 ppm

Leaves of root and tuber vegetables (human food or animal feed) (crop group 2)...............0.04 ppm

Nongrass animal feeds (forage, fodder, straw, and hay) (crop group 18), forage ................0.06 ppm

Nongrass animal feeds (forage, fodder, straw, and hay) (crop group 18), hay.....................0.15 ppm

Peanut hay ............................................................................................................................0.03 ppm

Root and tuber vegetables, root vegetables (crop subgroup 1A)..........................................0.03 ppm

Plant metabolism studies were conducted in rice, lettuce, cotton, and tomato, representing 

cereals, leafy, oilseeds, and fruit groups. Results indicate that plant metabolism is similar 

following foliar and soil application. The metabolic fate of this insecticide is complex with the 

formation of many low level metabolites. However, cyantraniliprole is the major component in 

rice, lettuce, cotton, and tomato. Results in crop field trials confirm that the parent compound is 

the predominant residue.

In rotational crops, parent is the major residue present in soybean, wheat grain, lettuce, and beet 

root. As in primary crops, there exist many minor metabolites. In livestock feed stuffs such as 

wheat forage, hay, straw, and soybean foliage, parent plus one or two metabolites (IN-J9Z38, INJSE76, and/or IN-MLA84) are present as major residues. 

The metabolism in livestock is extensive and the amount of unchanged parent present in milk, 

eggs, and tissues is matrix-dependent. The major components present in various tissues include a 

combination of parent, IN-N7B69, IN-J9Z38, IN-MLA-84, and/or IN-MYX98.

The results of processing studies indicate the formation of three major degradates (IN-J9Z38, INN5M09, and IN-F6L99) under conditions of heat and/or hydrolysis. All three degradates are 

present in apple sauce, whereas only IN-J9Z38 is present in cooked spinach, orange oil, tomato 

paste, cottonseed oil, and cottonseed meal.

Storage stability data have been submitted for residues of cyantraniliprole and various 

metabolites in five categories of plant commodity matrices and in milk and eggs. The intervals 

of demonstrated storage stability cover the actual intervals of frozen storage of plant and 

livestock commodity samples from the crop field trials, processing studies, and livestock feeding 

studies. No corrections for loss during storage are needed for any plant or livestock commodity 

for purposes of tolerance considerations.

Based on the livestock metabolism studies which did not show a change in the metabolic profile 

after 10 months of frozen storage for eggs, muscle and fat of hens and 6 months of frozen storage 

for milk, fat, kidney and liver of goats, and since the egg, milk, and tissue samples from the 

livestock feeding studies were stored frozen and analyzed within 30 days of collection, HED 

concludes that storage stability data for the livestock matrices are unnecessary.

Adequate field trial data are available to support the proposed uses on Brassica (cole) Leafy 

Vegetables (Group 5), Bulb Vegetables (Group 3-07), Bushberries (Group 13-07B), Oilseed 

(Group 20), Citrus Fruit (Group 10-10), Cucurbit Vegetables (Group 9), Fruiting Vegetables 

(Group 8-10), Leafy Vegetables (non-Brassica) (Group 4), Pome Fruit (Group 11-10), Stone 

Fruit (Group 12-12), Tree Nuts (Group 14), and Tuberous and Corm Vegetables (Subgroup 1C). 

There are no data to support use in food/feed handling, storage, and transport facilities.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Methods for measuring cyantraniliprole include EU multi-residue method DFG S19 (LC/MS/MS 

module, Dupont-21328) and NAFTA LC/MS/MS 1187 & 1552 methods. These methods utilize 

two mass transitions per analyte, which is sufficiently specific so that confirmatory methods are 

not required. NAFTA method 1187 has been independently validated with a limit of 

quantification (LOQ) of 0.01 ppm parent and its metabolites in almond, onion, tomato paste and 

sun dried tomatoes. Method 1552 has been independently validated with an LOQ of 0.01 ppm 

parent and its metabolites in milk, muscle and kidney. DFG S19 has been independently 

validated with an LOQ of 0.01 ppm parent for cereals and dry products, matrices with high water 

content, acidic matrices and fatty product.

Cyantraniliprole is not recovered using the FDA multi-residue methods. Cyantraniliprole is 

successfully recovered and quantitiated by the European DFG method.

Processing studies have been submitted for several crops including potato, tomato, orange, apple, 

plum, and cottonseed. No tolerances are needed for most processed commodities, as the 

cyantraniliprole concentration did not increase from the raw agricultural commodity (RAC) to 

the processed commodity. Cyantraniliprole did concentrate in tomato dry pomace, sundried 

tomatoes, orange oil, apple puree, apple dry pomace (but not wet), and dried plums. Dried plums 

and citrus oil are recognized processed commodities for purposes of tolerances.

Adequate cattle and poultry feeding studies have been submitted. The data indicate that 

tolerances for milk and the fat, meat, and meat byproducts of cattle, goat, horse, and sheep are 

needed to support the proposed cyantraniliprole uses, but tolerances are not required for hogs and 

poultry commodities. 

The confined rotational crop studies are adequate. Adequate limited and extended field 

rotational crop studies have been submitted. The studies indicate non-quantifiable levels of 

parent residues in/on cereal grains, soybean seed, and legumes, and quantifiable levels in leafy 

vegetables, foliage of legume vegetables, and some root commodities, at a plantback interval 

(PBI) of 30 days. The studies also indicate quantifiable levels of parent residues in livestock feed 

stuffs such as alfalfa, grass hay, and soybean hay at intervals up to 365 days. Inadvertent or 

indirect tolerances for these rotational crops are needed at a PBI of 30 days. 

Currently there are no Codex MRLs/tolerances for cyantraniliprole. Therefore, there are no 

issues with harmonization of tolerances. Canadian MRLs are being proposed concurrently with 

US tolerances and are harmonized for primary crop commodities and livestock commodities 

except fruiting vegetables. The US fruiting vegetables tolerance is harmonized with the 

European Union.

Regulatory Recommendations and Residue Chemistry Deficiencies

HED has examined the residue chemistry database for cyantraniliprole. Pending submission of a 

revised Section B (see requirements under Directions for Use), a revised Section F (see 

requirements under Proposed Tolerances and Table 140), and analytical reference standards (see 

requirements under Submittal of Analytical Reference Standards), there are no residue chemistry 

issues that would preclude granting Section 3 registration for the requested crop commodity uses

of cyantraniliprole (except food/feed establishment uses), or establishment of tolerances for 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 9 of 193

residues of cyantraniliprole. It is recommended that tolerances be established for residues of the 

insecticide cyantraniliprole (3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-

[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide), including its metabolites and 

degradates, in or on the plant commodities specified below and detailed in Table 140. 

Compliance with the tolerance levels specified is to be determined by measuring only 

cyantraniliprole in or on the commodity.

Almond, hulls.........................................................................................................................8.0 ppm

Bushberry subgroup 13-07B ..................................................................................................4.0 ppm

Brassica, head and stem, subgroup 5A...................................................................................3.0 ppm

Brassica, leafy greens, subgroup 5B .......................................................................................30 ppm

Onion, bulb, subgroup 3-07A...............................................................................................0.04 ppm

Onion, green, subgroup 3-07B ...............................................................................................8.0 ppm

Cherry subgroup 12-12A........................................................................................................6.0 ppm

Fruit, citrus group 10-10.......................................................................................................0.70 ppm

Citrus, oil................................................................................................................................2.4 ppm

Cotton, gin byproducts............................................................................................................10 ppm

Vegetable, cucurbit, group 9 ................................................................................................0.40 ppm

Vegetable, fruiting, group 8-10 ..............................................................................................2.0 ppm

Vegetable, leafy, except Brassica, group 4 .............................................................................20 ppm

Oilseed group 20 ....................................................................................................................1.5 ppm

Fruit, pome, group 11-10........................................................................................................1.5 ppm

Vegetable, tuberous and corm, subgroup 1C........................................................................0.15 ppm

Peach subgroup 12-12B..........................................................................................................1.5 ppm

Plum subgroup 12-12C..........................................................................................................0.50ppm

Nut, tree, group 14................................................................................................................0.04 ppm

It is recommended that tolerances be established for residues of the insecticide cyantraniliprole 

(3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1Hpyrazole-5-carboxamide), including its metabolites and degradates, in or on the livestock 

commodities below and in Table 140. Compliance with the tolerance levels specified below is 

to be determined by measuring only cyantraniliprole in or on the commodity.

Cattle, fat..............................................................................................................................0.01 ppm

Cattle, meat ..........................................................................................................................0.01 ppm

Cattle, meat byproducts........................................................................................................0.01 ppm

Goat, fat................................................................................................................................0.01 ppm

Goat, meat ............................................................................................................................0.01 ppm

Goat, meat byproducts..........................................................................................................0.01 ppm

Horse, fat..............................................................................................................................0.01 ppm

Horse, meat ..........................................................................................................................0.01 ppm

Horse, meat byproducts........................................................................................................0.01 ppm

Milk......................................................................................................................................0.01 ppm

Sheep, fat..............................................................................................................................0.01 ppm

Sheep, meat ..........................................................................................................................0.01 ppm

Sheep, meat byproducts........................................................................................................0.01 ppm

It is recommended that indirect or inadvertent tolerances be established for residues of 

insecticide cyantraniliprole (3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-

[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide), including its metabolites and 

degradates, in or on the commodities below and in Table 140. Compliance with the tolerance 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 10 of 193

levels specified below is to be determined by measuring only cyantraniliprole in or on the 

commodity.

Vegetable, foliage of legume, group 7 .................................................................................0.70 ppm

Grain, cereal, forage, fodder and straw, group 16 ................................................................0.50 ppm

Grass, forage, fodder and hay, group 17 ..............................................................................0.50 ppm

Vegetable, leaves of root and tuber, group 2........................................................................0.04 ppm

Animal feed, nongrass, group 18..........................................................................................0.20 ppm

Peanut, hay ...........................................................................................................................0.01 ppm

Vegetable, root, subgroup 1A...............................................................................................0.02 ppm

A human health risk assessment is forthcoming.

Pre-Registration Data Needs

860.1200 Directions for Use

The use in food/feed handling establishments is not supported by the required studies of 

OCCPS Guideline 860.1460. Therefore, all uses related to treatment of food and feed 

handling/storage/ transportation are not allowed. This applies specifically to DuPont 

HGW86 suspension concentrate 352-IAI.

The rotational crop restrictions must be amended, as follows:

(1) Cereal grains (crop group 15) may not be planted immediately after use of 

cyantraniliprole. Cereal grain (crop group 15) must have a 30 day plantback interval 

(PBI). This is to be consistent with the 30 day PBI for crop group 16 (forage, fodder, 

and straw of cereal grains).

(2) Legume vegetables (crop group 6) may not be planted immediately after use of 

cyantraniliprole. Legume vegetables (crop group 6) must have a 30 day PBI. This is 

to be consistent with the 30 day PBI for crop group7 (foliage of legume vegetables).

(3) Tuberous and corm vegetables (subgroup 1C) may not be planted immediately after 

use of cyantraniliprole. The subgroup may be expanded to the entire tuberous and 

corm vegetables group with a PBI of 30 days. Although a primary crop use exists for 

subgroup 1C for the roots, the PBI of 30 days is needed to cover the foliage (under 

crop group 2).

860.1550 Proposed Tolerances

A revised Section F should be submitted reflecting the tolerances and commodity 

definitions that appear in Table 140. The proposed tolerances for almond hulls, 

Brassica head and stem (crop subgroup 5A), cattle liver, citrus raw peel, cucurbit 

vegetables (crop group 9), goat liver, sheep liver, horse liver, hog fat, hog liver, hot 

meat, hog byproducts, leafy vegetables except Brassica (crop group 4), milk fat, 

oilseeds (crop group 20), pome fruits (crop group 11-10), potato wet peel, stone fruits 

except cherry (crop group 12-12), citrus oil, and tree nuts (crop group 14), need to be 

modified. The recommended primary plant commodity tolerances are derived from 

use of the Organization of Economic Cooperation and Development (OECD) 

statistical calculation procedure. The livestock commodity tolerances are derived 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 11 of 193

from consideration of the maximum reasonalbly balanced livestock diets and the 

livestock feeding studies. The recommended processed commodity tolerance is based 

on the median processing factor and the HAFT (highest average field trial). The 

proposed tolerances for inadvertent residues for group 7, group 16, group 17, group 

18, peatnut hay, and subgroup 1A, with a PHI of 30 days, need to be revised to 

reflect the recommended tolerances based on the use of the OECD statistical 

calculation procedure. These calculation procedures explain the differences observed 

in proposed and recommended tolerances. . For some crop groups, more than one 

tolerance was proposed for various components of the group, for example, crop group 

6 (forage, fodder, and straw of cereal grains). Only one tolerance is possible per 

group, and the tolerance is based on the member commodity with the highest residue 

levels.

860.1650 Submittal of Analytical Reference Standards

analytical reference standards for cyantraniliprole must be submitted to the 

Analytical Chemistry Lab, which is located at Fort Meade, to the attention of either 

Theresa Cole or Thuy Nygen at the following address:

USEPA

National Pesticide Standards Repository/Analytical Chemistry 

Branch/OPP

701 Mapes Road

Fort George G. Meade, MD 20755-5350

Condition of Registration Data Needs

There are no conditions of registration data needs. 

Background

The chemical names and structures of major cyantraniliprole metabolites are presented in 

Appendix I.

Table 1. Chemical Nomenclature.

Chemical structure

Br

Cl

O

O

NH

N

N

HN

N

N

CH3

CH3

Common name Cyantraniliprole

Company experimental name Cyantraniliprole; DPX-HGW86
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 12 of 193

Table 1. Chemical Nomenclature.

IUPAC name 3-Bromo-1-(3-chloro-2-pyridyl)-4’-cyano-2’-methyl-6’-(methylcarbamoyl)-

pyrazole-5-carboxanilide

CAS name 3-Bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-

[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide

CAS registry number 736994-63-1

Formula C19H14BrClN6O2

End-use product (EP) 352-IAG, 18.66% SX; 352-IAI, 18.66% SC; 352-IAN, 18.66% SC; 352-IAT, 

50% FS; 352-ILT, 50% FS; 352-ILD, 10.2% SE; 352-ILT, 10.26% OD.

Table 2. Physicochemical Properties of the Technical Grade of Cyantraniliprole.

Parameter Value Reference

Physical state White fine powder solid MRID 48208438

Melting point 224 oC MRID 48208438

Density 1.4965 g/cm3at 20 oC MRID 48208438

Water solubility (20 °C) 14.24 mg/L MRID 48208438

Solvent solubility (g/L at 20 °C) Acetone 6.54

Ethyl acetate 1.96

Dichloromethane 5.05

Toluene 0.576

n-Octanol 0.79

Methanol 4.73

o-Xylene 0.29

Acetonitrile 2.45

MRID 48208438

Vapor pressure 5.13 x 10-15 Pa at 20 oC MRID 48208438

Henry’s Law Constant (at 20 °C) pH7 = 1.7 x 10-18 atmosphere.m3/mole MRID 48208438

Octanol/water partition coefficient Log Kow = 1.94 + 0.11 at 20 oC MRID 48208438

Hydrolysis and photolysis pH 4: Stable

pH 7: Stable

pH 9: Hydrolyzed very rapidly with a half-life 

of <1 day. One major metabolite was 

observed, IN-J9Z38.

Photolysis (DT50): 0.233 days at 40o latitude in 

the summer to 4.12 days at 60o latitude in the 

winter

MRID 48208438

Dissociation constant (pKa) 8.80 + 1.38 at 20 oC MRID 48208438

860.1200 Directions for Use

The petitioner has submitted draft specimen labels for the end-use products containing 

cyantraniliprole listed in Table 3. The products include suspoemulsion (SE), suspension

concentrate (SC), flowable suspension (FS), and oil dispersion (OD) formulations from DuPont. 

A summary of the proposed use patterns on food and feed crops is detailed in Table 4.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 13 of 193

Table 3. Summary of Proposed End-Use Products for Agricultural and Food/Feed Use.

Trade Name File 

Symbol

No.

ai (% of 

formulation)

Formulation 

Type

Target Crops Label

Date

DuPont HGW86

GH & N Insect 

Control

352-IAG 18.66

(1.67 lb 

ai/gallon)

SC

(suspension 

concentrate)

Vegetable transplants in greenhouses

(fruiting vegetables, leafy vegetables, 

tuberous and corm vegetables)

-

DuPont HGW86 

SC Insect 

Control

352-IAI 18.66

(1.67 lb 

ai/gallon)

SC

(suspension 

concentrate)

Residential, Commercial, and Livestock

buildings and transport, including food and 

feed.

-

DuPont 

VerimarkTM 

Insect Control

352-IAN 18.66

(1.67 lb 

ai/gallon)

SC

(suspension 

concentrate)

Brassica vegetables

Cucurbit vegetables

Fruiting vegetables

Leafy vegetables

Tuberous and corm vegetables

Citrus

-

DuPont 

DermacorTM Z103 Insecticide 

Seed Treatment

352-ILI 50.

(5.21 lb 

ai/gallon)

FS

(flowable 

suspension)

Rapeseed (including canola)

Mustard seed

-

DuPont ExirelTM 

Insect Control

352-ILO 10.20

(0.83 lb 

ai/gallon)

SE

(suspoemuls

ion or oil in 

water)

Brassica (Cole) leafy vegetables

Bulb vegetables

Cucurbit vegetables

Fruiting vegetables

Commercial greenhouse grown (to 

harvest): eggplant, pepper, tomato

Leafy vegetables

Bushberries (subgroup 13-7B)

Citrus fruit

Pome fruit

Stone fruit

Tree nuts

-

DuPont 

BeneviaTM

Insect Control

352-ILT 10.26%

(0.83 lb 

ai/gallon)

OD

(oil 

dispersion)

Bulb vegetables

Cotton

Oil seed crops

Tuberous and corm vegetables

Tree nuts

-

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

Brassica (cole) Leafy Vegetables (Group 5)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.045 –

0.133 N/S 0.4 5 1

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 14 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

non-ionics; 

organosilicones/organosilicat

es).

For diamondback moth, do 

NOT apply more than twice 

to any generation within a 30 

day period nor more than 6 

applications per year.

Soil at 

Planting (infurrow spray, 

transplant 

water 

treatment, hill 

drench, 

surface band, 

soil shank 

injection)

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.065 –

0.130 1

0.176 at-plant;

0.4 all N/A N/A

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

For diamondback moth, do 

NOT apply(all 

cyantraniliprole 

formulations) more than 

twice to any generation 

within a 30 day period nor 

more than 6 applications per 

year.

Bulb Vegetables (Group 3-07)

Foliar 

(ground, 

aerial)

Chemigation 

(overhead 

only)

10.26% OD

(BeneviaTM)

0.088 –

0.133 N/S 0.4 5 1

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.088 –

0.133 N/S 0.4 5 1

Bushberries (Group 13-07B)

Foliar 

(ground, 

aerial)

10.20% SE

(susp 

emulsion)

(ExirelTM)

0.065 –

0.133 N/S 0.4 5 3

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Do NOT apply dilute 

applications of more than 

200 gallons per acre. 

Do NOT apply less than 30 

gallons of water per acre

Canola (Rapeseed)

Seed 

treatment

50% FS

(flowable 

suspension)

0.15 –

1.0 lb 

ai/cwt

1 0.4 all N/A N/A

Apply as a water-based 

slurry.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 15 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

Dermacor®

Z-103

Citrus Fruit (Group 10-10)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemulsion)

(ExirelTM)

0.088 –

0.133 N/S 0.4 7 1

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es).

For best results, use 100 –

150 gallons of water per acre 

with airblast equipment, but 

not less than 30 gallons of 

water per acre.

Do NOT apply less than 2 

gallons of water per acre 

with fogger equipment for 

Asian citrus psyllid contro. 

Soil drench or 

microsprinkler 

chemigation

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.261 –

0.391 1 0.4 all N/A N/A

For use on citrus nursery 

stock (trees less than 4 years 

old).

Do NOT make aerial or 

airblast applications.

Soil drench should be in a 

minimum of 1⁄2 to 1 pint of 

water per tree distributed 

evenly around the tree root 

zone.

Microsprinkler chemigation 

application pattern must 

include a majority of the 

tree’s root zone.

Cotton

Foliar 

(ground, 

aerial)

10.26% OD

(BeneviaTM)

0.045 –

0.133 N/S 0.4 7 7`

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Cucurbit Vegetables (Group 9)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemulsion)

(ExirelTM)

0.045 –

0.133 N/S 0.4 1 1

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 16 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Soil at 

Planting (infurrow spray, 

transplant 

water 

treatment, hill 

drench, 

surface band, 

soil shank 

injection)

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.065 –

0.176 1

0.176

0.4 all N/A 1

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

Drip 

chemigation

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.065 –

0.130

2

(1 if atplant 

application 

made)

0.26

0.4 all 10 1

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

Must be applied uniformly in 

the root zone.

Make application up to peak 

bloom crop stage, about 40 

days after crop emergence or 

transplant

Fruiting Vegetables (Group 8-10)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.045 –

0.133 N/S 0.4 1 5

May be used in greenhouses 

for the production of 

eggplant, pepper, tomato.

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Foliar 

(greenhouse)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.088 –

0.133 N/S 0.4 1 1

Eggplant, pepper, tomato.

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 17 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

Soil at 

Planting (infurrow spray, 

transplant 

water 

treatment, hill 

drench, 

surface band, 

soil shank 

injection)

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.065 –

0.176 1

0.176

0.4 all N/A N/A

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

Drip 

chemigation

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.065 –

0.130

2

(1 if atplant 

application 

made)

0.26

0.4 all 10 1

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

Must be applied uniformly in 

the root zone.

Leafy Vegetables (non-Brassica) (Group 4)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.045 –

0.133 N/S 0.4 5 1

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Soil at 

Planting (infurrow spray, 

transplant 

water 

treatment, hill 

drench, 

surface band, 

soil shank 

injection)

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.065 –

0.176 1

0.176

0.4 all N/A N/A

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

Mustard Seed

Seed 

treatment

50% FS

(flowable 

suspension)

Dermacor®

Z-103

0.15 –

1.0 lb 

ai/cwt

1 0.4 all 7 7

Apply as a water-based 

slurry.

Oil Seed Crops (Group 20) (see also rapeseed)

Foliar 

(ground, 

aerial)

10.26% OD

(BeneviaTM)

0.045 –

0.133 N/S 0.4 7 7

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 18 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Pome Fruit (Group 11-10)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.055 –

0.133 N/S 0.4 7 3

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es).

Do NOT apply dilute 

applications of more than 

200 gallons of water per 

acre. 

Do NOT apply less than 30 

gallons of water per acre,

Rapeseed

Seed 

treatment

50% FS

(flowable 

suspension)

Dermacor®

Z-103

0.15 –

1.0 lb 

ai/cwt

1 0.4 all 7 7

Apply as a water-based 

slurry.

Stone Fruit (Group 12)

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.065 –

0.133 N/S 0.4 7 3

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es).

Do NOT apply dilute 

applications of more than 

200 gallons of water per 

acre. 

Do NOT apply less than 30 

gallons of water per acre,

Tree Nuts (Group 14) and Pistachio

Foliar 

(ground, 

aerial)

10.26% OD

(BeneviaTM)

0.055 

0.133 N/S 0.4

7

(14 

coddli

ng 

moth)

5

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Do NOT apply dilute 

Foliar 

(ground, 

aerial)

10.20% SE

(suspoemuls

ion)

(ExirelTM)

0.055 

0.133 N/S 0.4

7

(14 

coddli

ng 

moth)

5
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 19 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

application of more than 300 

gallons of water per acre.

Do NOT apply less than 30 

gallons of water per acre.

Tuberous and Corm Vegetables (Subgroup 1C)

Foliar 

(ground, 

aerial)

Chemigation 

(overhead 

only)

10.26% OD

(BeneviaTM)

0.045 –

0.133 N/S 0.4 5 7

Do not USE in greenhouses 

for transplant production;

Do NOT apply with drip 

irrigation;

Do NOT apply to soil.

Adjuvants may be used 

(methylated seed oils; 

petroleum oil concentrates: 

non-ionics; 

organosilicones/organosilicat

es)

Do NOT apply more than 

twice to a generation of 

Colorado potato bettle.

For potato tuberworm, avoid 

treating successive

generations with the same 

mode of action pesticide.

Soil at 

Planting (infurrow spray, 

transplant 

water 

treatment, hill 

drench, 

surface band, 

soil shank 

injection)

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.088 -

0.176 1

0.176

0.4 all N/A N/A

Do not USE in greenhouses 

for transplant production;

Do NOT make aerial or 

airblast applications.

Potato seed 

piece 

treatment

18.66% SC 

(suspension 

concentrate)

(Verimark

TM)

0.131 –

0.176

[0.006 –

0.010 lb

ai/ cwt]

1

0.176

0.4 all N/A N/A

Apply as a water-based

slurry prior to planting. Use 

approved dye or colorant.

Food and Feed Handling Establishments

Crack and 

crevice, 

including 

indoor areas 

with exposed 

food/feed 

present.

For use in 

residential 

buildings,

schools, 

institutional, 

commercial, 

18.66% SC 

(suspension 

concentrate)

HGW86

0.018 –

0.035 lb 

ai/1000 

sq ft

As needed Not specified 7 N/A

Avoid spraying food and 

feed handling equipment.

Do not apply to areas that are 

routinely washed such as

cracks and crevices in tops of 

tables, food/feed preparation

and prepared food holding 

surfaces.

Do not apply a broadcast or 

general surface application to

interior surfaces of 

residential or commercial 

structures.

Do not apply this product to 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 20 of 193

Table 4. Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Application

Timing, Type, 

and Equip.

Formulation Applic. 

Rate 

(lb ai/A)

Max. No. 

Applic. per 

Season

Max. Seasonal 

Applic. Rate

(lb ai/A)

RTI 

(days)

PHI

(days)

Use Directions and 

Limitations

agricultural 

and industrial

facilities 

(including 

warehouses, 

apartments, 

supermarkets,

restaurants, 

hospitals, and 

food

handling/stora

ge/processing 

establishments

, animal 

production

facilities, 

feedlots, 

broiler houses, 

livestock 

barns, pet

kennels) and 

transportation

equipment.

pets, edible crops, or sources

of electricity.

Do not make applications to 

structures while occupied by

livestock, poultry or 

companion animals.

Do not apply to milk rooms 

or feed rooms.

Do not make applications to 

any animal feedstuffs, water,

or watering equipment.

Do not contaminate any 

animal food, feed, or water 

in and around livestock, 

poultry, or companion 

animal housing when making 

applications.

Each DuPont end-use product label contains similar crop rotation instructions, as follows:

Crops on this label and the following crops or crop groups may be planted immediately following the last 

application of this product:

Brassica Leafy Vegetables (Crop Group 5); 

Bulb Vegetables (Crop Group 3- 07); 

Bushberries (Berry

and Fruit Crop Group subgroup 13- 07B); 

Cereal Grains (Crop Group 15); 

Citrus (Crop Group 10-10); 

Cotton; 

Cucurbit Vegetables (Crop Group 9);

Fruiting Vegetables (Crop Group 8-10);

Leafy Vegetables (except Brassicas) (Crop Group 4);

Legume Vegetables (succulent or dried) (Crop Group 6);

Low Growing Berries (Berry and Fruit Crop Group subgroup 13-07G); 

Oilseeds (Crop Group 20); 

Pome Fruits (Crop Group 11-10);

Tuberous and Corm Vegetables (Root and Tuber Vegetables Crop Group subgroup 1C); 

Stone Fruits (Crop Group12); 

Tree Nuts (Crop Group 14).

The following crops or crop groups may be planted 30 days following the last application of the product:

Foliage of Legume Vegetables (Crop Group 7);

Forage, Fodder and Straw of Cereal Grains (Crop Group 16); 

Grass Forage, Fodder and Hay (Crop Group 17);
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 21 of 193

Nongrass Animal Feeds (forage, fodder, straw and hay) (Crop Group 18); 

Peanuts; 

Leaves of Root and Tuber Vegetables (Crop Group 2); 

Root Vegetables (Root and Tuber Vegetables Crop Group subgroup 1A).

All other crops cannot be planted until 12 months after the last application of the product.’

As cereal grains and forage, fodder, hay, and straw of cereal grains are all part of the same cereal 

crop, there cannot be separate PBIs for the grains and the livestock commodities (forage, fodder, 

hay, and straw of cereal grains). Cereal grains (crop group 15) are subject to the 30 day PBI and 

may not be planted immediately following the last application of cyantraniliprole. Similar 

considerations apply to Legume Vegetables (succulent or dried) (Crop Group 6) and Tuberous 

and Corm Vegetables (Root and Tuber Vegetables Crop Group subgroup 1A), where 30 day 

PBIs may be used.

Conclusions. The use directions are adequate to allow evaluation of the residue data relative to 

the proposed uses in the U.S. The proposed maximum seasonal rate and minimum RTIs are 

supported by the submitted field trial data. The proposed minimum PHIs are supported by the 

submitted field trial data. The proposed plantback intervals are supported by the rotational crop 

field trial data.

The rotational crop restrictions must be amended, as follows: 

(1) Cereal grains (crop group 15) may not be planted immediately after use of 

cyantraniliprole. Cereal grain (crop group 15) must have a 30 day plantback interval 

(PBI). This is to be consistent with the 30 day PBI for crop group 16 (forage, fodder, 

and straw of cereal grains).

(2) Legume vegetables (crop group 6) may not be planted immediately after use of 

cyantraniliprole. Legume vegetables (crop group 6) must have a 30 day PBI. This is 

to be consistent with the 30 day PBI for crop group7 (foliage of legume vegetables).

(3) Tuberous and corm vegetables (subgroup 1C) may not be planted immediately after 

use of cyantraniliprole. The subgroup may be expanded to the entire tuberous and 

corm vegetables group with a PBI of 30 days. Although a primary crop use exists for 

subgroup 1C for the roots, the PBI of 30 days is needed to cover the foliage (under 

crop group 2). For example, sweet potato is a member of crop group a 1C, and sweet 

potato leaves are in crop group 2. 

Many of the field trials with foliar application were conducted with an OD formulation, but the 

end-use product labels are for an SE formulation. Both are dispersions or suspensions in oil to 

be diluted in water prior to application. Therefore, the formulations are considered equivalent, 

and bridging trials will not be needed. Side-by-side trials show similar residues for the two 

formulations when applied at the same rate, for example, Brassica flowerhead vegetables and 

leaf and head lettuce.

860.1300 Nature of the Residue - Plants

Monograph for Cyantraniliprole, Sections B.7.1.1 through B.7.1.4; B.7.1.6 (MRIDs 48119988-48119990, 

48122525)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 22 of 193

The petitioner submitted studies pertaining to the metabolism of cyantraniliprole in cotton, 

lettuce, tomato, and rice. Two different radiolabels were used in the plant studies, either in the 

cyano group [CN] or in the carbonyl next to the pyrazole ring [PC]. The label positions are 

presented below:

1. [CN14C]-cyantraniliprole

2. [PC14C]-cyantraniliprole

Numerous metabolites were identified in these studies. The chemical structures and report 

names for these metabolites are provided in Appendix I. 

Cotton

MRID 48119988: Three 150 g ai/ha applications were made to cotton via foliar or soil drench 

application. Foliar applications were made to plants 3 weeks post-emergence and at 7 and 14 

days after the initial application (BBCH 16, 18 and 19). Soil drench applications were made 7 

weeks post-emergence and at 7 and 14 days after the first application (BBCH 19 to 51).

TRRs after soil drench treatment were 0.01 ppm or less in all immature leaf samples, <0.01 ppm 

in lint and seed, and 0.02-0.10 ppm in cotton gin byproducts. TRRs after foliar treatments ranged 

from 2.71-14.53 ppm at day 0 after the first treatment and 0.42 ppm in leaves 13 days after the 

last treatment, and 0.13 ppm in gin byproducts, 0.01 ppm in lint and <0.01 ppm in seed at 

harvest (125 days after the last treatment).

Cyantraniliprole was the major residue in cotton leaves and gin byproducts. Additional 

metabolites include: IN-DBC80, IN-J9Z38, IN-JCZ38, IN-JSE76, IN-K5A77, IN-K7H19, INMLA84, IN-MYX98, IN-N7B69, IN-NXX70, and IN-QKV54.

Table 5. Distribution of residues in leaves and cotton gin by-products following three foliar applications of [CN/ PC14C]-

cyantraniliprole

0 DAT1

a

7 DAT1 7 DAT2 7 DAT3 13 DAT3

124 DAT3

Gin byproducts

TRR, mg/kg 2.709 5.414 2.654 0.505 0.425 0.131

mg/kg (% TRR)

Extracted residues 86.8% 100% 96.7% 97.8% 97.9% 83.8%

NH

O

HN

O N

N

Br

N

Cl

NC

1

2
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 23 of 193

Table 5. Distribution of residues in leaves and cotton gin by-products following three foliar applications of [CN/ PC14C]-

cyantraniliprole

0 DAT1

a

7 DAT1 7 DAT2 7 DAT3 13 DAT3

124 DAT3

Gin byproducts

Unextracted residues 13.2% <0.1% 3.4% 2.2% 2.0% 16.2%

Extracted residues after 

processing and treatment with 

acid, alkali and enzymes

2.338

(86.3%)

5.231

(96.6%)

2.399

(90.4%)

0.454

(89.9%)

0.429

(100.9%)

0.103

(79.0%)

Loss during processing 0.014

(0.5%)

0.179

(3.3%)

0.165

(6.2%)

0.040

(7.9%)

-0.012

(-2.9%)

0.006

(4.8%)

Cyantraniliprole 1.889

(69.7%)

1.066

(19.7%)

1.873

(70.7%)

0.187

(37.3%)

0.115

(27.1%)

0.043

(34.4%)

IN-DBC80 NDb 0.030

(0.6%) ND ND ND ND

IN-J9Z38 0.028

(1.0%)

0.190

(3.5%)

0.078

(2.9%)

0.011

(2.3%)

0.006

(1.5%)

0.008

(5.7%)

IN-JCZ38 ND 0.069

(1.3%) ND ND ND 0.007(6.1%)

IN-JSE76 ND 0.039

(0.7%)

0.003

(0.1%) ND ND ND

IN-K5A77 ND 0.050

(0.9%) ND ND ND ND

IN-K7H19 ND 0.089

(1.7%)

0.018

(0.7%)

0.019

(4.1%)

0.021

(4.9%) ND

IN-MLA84 0.091

(3.3%)c

0.049

(0.8%)

0.013

(0.5%)c

0.017

(3.5%)

0.014

(3.3%)c NDc

IN-MYX98 ND 0.106

(2.0%) ND ND ND ND

IN-N7B69 ND 0.017

(0.3%) ND ND 0.005(1.1%)

0.001

(1.2%)

IN-NXX70 --

1.172

(21.7%) --

0.006

(1.1%) -- --

IN-QKV54 ND 0.557

(10.3%)

0.039

(1.5%)

0.025

(5.0%)

0.016

(3.8%) ND

Unidentified 0.120

(4.4%)d

0.749

(13.9%)e

0.167

(6.9%)f

0.122

(25.2%)g

0.176

(41.3%)h

0.002

(7.3%) i

Total Characterized/Identified 2.120

(78.4%)

4.312

(78.2%)

2.211

(83.3%)

0.399

(79%)

0.352

(82.9%)

0.072

(54.7%)

Uncharacterized 0.214

(7.9%)

0.996

(18.4%)

0.188

(7.1%)

0.055

(10.9%)

0.076

(18.0%)

0.032

(24.3%)

a: DAT1 = days after treatment 1 ; DAT2 = days after treatment 2; DAT3 = days after treatment 3

b: ND = Not detected

c: Corresponds to a mixture of IN-MLA84 and IN-NXX70 (which co-eluted in the HPLC system)

d: 8 components; none greater than 0.035 mg/kg (1.3%TRR)

e: 47 components; none greater than 0.163 mg/kg (3.0%TRR)

f: 28 components; none greater than 0.047 mg/kg (1.8%TRR) 

g: 32 components; none greater than 0.031 mg/kg (6.2%TRR)

h: 31 components; none greater than 0.042 mg/kg (9.9%TRR)

i: 20 components; none greater than 0.001 mg/kg (0.9%TRR)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 24 of 193

Figure 1: Proposed Metabolic Pathway of Cyantraniliprole on Cotton

Lettuce

MRID 48119990: Three 150 g ai/ha applications were made to lettuce via foliar or soil drench 

application. Foliar applications were made to plants 3 weeks post-emergence (BBCH 14/15 

equivalent to BBCH 33 for fruit development) and at 7 (BBCH 16 equivalent to BBCH 35) and 

14 (BBCH 18 equivalent to BBCH 36) days after the initial application. Soil drench applications 

were made 7 weeks post-emergence (BBCH 18/19 equivalent to BBCH 36) and at 7 (BBCH 19 

equivalent to BBCH 37/38) and 14 (BBCH 19 equivalent to BBCH 37/38) days after the first 

application.

TRR following soil drench treatment declined from 0.14 ppm 7 days after the first application to 

0.01 ppm at harvest (32 days after the last application). Higher residues were observed 

following foliar application and residues declined from 10.84 ppm initially to 0.03 ppm at 

maturity.

N

N N

NH

O

Cl

Br

N

O

N

N

N

O

N

N

N

Br

Cl

N

O

H2N O

N

NH

O

N

N N

Cl

Br

NH

O

N

O

N

N N

Cl

Br

O

HO

N

N N

NH

O

Cl

Br

N

O

HO

N

NH

O

N

N

N

Br

Cl

N

H

O OH

N

NH

N

O

N

N

N

Br

Cl

N

OH

O

N

N

N

Br

Cl

O

H2N

N

N N

N

H

O

NH2

O

Br

Cl

NH

O

N

N

N

Br

Cl

NH2

O

N

N

N

O

N

N

N

Br

OH

N

N

N

O

N

N

H

Br

N

O

H2N N

N

O

N

N N

Br

Cl

Cyantraniliprole

(DPX-HGW86)

IN-J9Z38

IN-JCZ38

IN-JSE76

IN-N7B69

IN-MYX98

IN-MLA84

IN-DBC80

IN-K7H19

IN-HGW87

IN-NXX70

IN-QKV54

IN-K5A77
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 25 of 193

Cyantraniliprole was the major residue in lettuce. Additional metabolites include: IN-K5A79, 

IN-MLA84, IN-NXXX70, IN-J9Z38, IN-DBC80, IN-N7B69, IN-K5A79, IN-JSE76, INHGW87, IN-K5A77, and IN-QKV54. IN-J9Z38 was a major metabolite in mature lettuce from 

foliar treatment.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 26 of 193

Table 6. Distribution of residues in lettuce following three foliar applications of [CN/PC14C]-cyantraniliprole

14C-Residue

Sampling point

0DAT1 7DAT1 0DAT2 7DAT2 0DAT3 7DAT3 14DAT3

32DAT3

Maturity

TRR, mg/kg 10.844 1.669 9.662 2.801 7.789 1.993 0.983 0.032

mg/kg (% TRR)

Extracted residues 99.9% 99.0% 98.6% 98.4% 97.7% 91.7%

Unextracted residues <0.1% 1.1% 1.3% 1.5% 2.3% 8.3%

Extracted residues after processing 

and treatment with acid, alkali and 

enzymes

10.790

(99.5%)

1.570

(94.1%)

Extraction 

not 

performed

2.812(100.4%)

Extraction 

not 

performed

1.771 

(89.0%)

0.936 

(95.2%)

0.030 

(92.2%)

Loss during processing 0.054

(0.5%)

0.080

(4.8%)

-0.048

(-1.7%)

0.189 

(9.5%)

0.025 

(2.5%)

<0.001

(-0.5%)

Cyantraniliprole 10.618

(97.9%)

1.320

(79.1%)

2.446

(87.3%)

1.564

(78.5%)

0.716

(72.6%)

0.016

(50.3%)

IN-DBC80 ND 0.009 

(0.5%)

0.041

(1.6%) 0.031

(1.6%)

ND ND

IN-N7B69 ND 0.017

(1.0%)

0.012

(0.4%) ND ND

IN-K5A79 ND 0.018

(1.2%) ND ND ND ND

IN-JSE76 ND 0.012

(0.7%) ND 0.014(0.7%) ND ND

IN-HGW87 ND 0.010

(0.6%) ND 0.018(0.9%) ND ND

IN-K5A77 ND ND 0.036

(1.2%) ND ND ND

IN-QKV54 ND ND 0.042

(1.4%)

0.017

(0.9%) ND ND

IN-MLA84/

IN-NXX70 a

0.034

(0.3%)

0.050

(3.0%)

0.058

(2.0%)

0.027

(1.4%)

0.027

(2.6%)

0.001

(4.9%)

IN-J9Z38 0.042

(0.4%) ND 0.028(1.0%)

0.021

(0.8%)

0.023

(2.3%)

0.011

(23.3%)

Unidentified 0.035

(0.3%)b

0.103

(6.6%)

c

0.117

(4.1%)d

0.078

(3.8%)e

0.077

(7.4%)f

<0.001

(1.3%)

Total Characterized/ Identified 10.729 

(98.9%)

1.539 

(92.7%) 2.780 (99%) 1.770 (88.6%)

0.843 

(84.9%)

0.028 

(79.8%)

Uncharacterized 0.065

(0.6%)

0.023

(1.4%)

0.039

(1.4%)

0.008

(0.4%)

0.101

(10.3%)

0.004

(12.4%)

a: HPLC method 1 did not resolve IN-MLA84 from IN-NXX70

b: 7 components, none greater than 0.006 mg/kg (0.1% TRR) 

c: 46 components, none greater than 0.009 mg/kg (0.6% TRR) 

d: 29 components, none greater than 0.011 mg/kg (0.4% TRR)

e: 27 components, none greater than 0.009 mg/kg (0.4% TRR)

f: 33 components, none greater than 0.007 mg/kg (0.7% TRR)

ND = Not detected
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 27 of 193

Figure 2: Proposed Metabolic Pathway of Cyantraniliprole on Lettuce

Tomato

MRID 48119989: Three 150 g ai/ha applications were made to tomatoes via foliar or soil drench 

application. Foliar applications were made to plants 3 weeks post-emergence (BBCH 14-15) and 

at 7 (BBCH 16) and 14 days (BBCH 53, 61) after the initial application. Soil drench applications 

N

N N

NH

O

Cl

Br

HN

O

N

N

N

O

N

N

N

Br

Cl

N

O

H2N O

HN

NH

O

N

N N

Cl

Br

NH

O

HN

O

N

N N

Cl

Br

O

HO

N

N N

NH

O

Cl

Br

HN

O

HO

N

NH

O

N

N

N

Br

Cl

N

H

O OH

N

NH

N

O

N

N

N

Br

Cl

N

OH

O

N

N

N

Br

Cl

O

H2N

N

N N

N

H

O

NH2

O

Br

Cl

O

H N2 N

N

O

N

N N

Br

Cl

NH

O

N

N

N

Br

Cl

NH2

O

N

O

HO

N

N N

N

H

O

NH2

O

Br

Cl

N

N

O

N

N

N

Br

OH

N

N

N

O

N

N

H

Br

N

Cyantraniliprole

(DPX-HGW86)

IN-J9Z38

IN-JCZ38

IN-JSE76

IN-N7B69

IN-MYX98

IN-MLA84

IN-DBC80

IN-K7H19

IN-K5A79

IN-K5A77

IN-HGW87

IN-NXX70 IN-QKV54
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 28 of 193

were made 7 weeks post-emergence (BBCH 19, 51) and at 7 (BBCH 51) and 14 days (BBCH 55, 

61) after the first application.

TRRs after soil drench treatment were 0.01 ppm in immature leaves 7 and 14 days after the third 

application, and <0.01 ppm in leaves and fruit at harvest (125 days after the third application. 

TRRs after foliar treatments ranged from 2.55-8.50 ppm at day 0 after the first treatment and 

declined to 1.30 ppm 14 days after the last application. Residues at harvest were <0.01 ppm in 

leaves and fruit (125 days after the last treatment).

Cyantraniliprole was the major residue in tomato leaves. Additional metabolites include: INK7H19, IN-JCZ38, IN-DBC80, IN-N7B69, IN-MYX98, IN-JSE76, IN-HGW87, IN-QKV54, 

IN-MLA84, IN-NXX70 and IN-J9Z38.

Table 7. Distribution of residues in tomato leaves following three foliar applications of [CN/PC14C]-

cyantraniliprole

14C-Residue Sample point

0DAT1 7DAT1 7DAT2 7DAT3 14DAT3

TRR, mg/kg 2.546 1.849 4.806 2.216 1.298

Extracted residues 99.0% 99.8% 100.0% 99.2% 97.7%

Unextracted residues 1.0% 0.1% 0.1% 0.8% 2.3%

Extracted residues after 

processing and treatment with 

acid, alkali and enzymes

2.683

(105.4%)

1.951

(105.5%)

4.878

(101.5%)

2.411

(108.8%)

1.328

(102.3%)

Loss during processing -0.163

(-6.4%)

-0.104

(-5.6%)

-0.077

(-1.6%)

-0.213

(-9.6%)

-0.059

(-4.6%)

Cyantraniliprole 2.429

(95.3%)

1.132

(61.1%)

4.154

(86.5%)

1.377

(62.2%)

0.562

(43.4%)

IN-K7H19 ND ND ND 0.025

(1.2%)

0.019

(1.4%)

IN-JCZ38 ND 0.019

(1.0%)

0.010

(0.2%)

0.022

(1.0%)

0.036

(2.8%)

IN-DBC80 ND 0.033

(1.9%)

0.021

(0.5%)

0.034

(1.5%) ND

IN-N7B69 ND ND 0.016

(0.3%)

0.019

(0.8%)

0.037

(2.9%)

IN-MYX98 ND ND ND 0.023

(1.0%)

0.018

(1.5%)

IN-JSE76 ND ND ND ND 0.012

(1.0%)

IN-HGW87 ND ND 0.028

(0.6%) ND ND

IN-QKV54 ND ND 0.040

(0.8%)

0.062

(2.8%)

0.054

(4.3%)

IN-MLA84/

IN-NXX70

0.023

(0.9%)

0.213

(11.5%)

0.149

(3.1%)

0.147

(6.6%)

0.074

(5.8%)

IN-J9Z38 0.022

(0.9%)

0.029

(1.6%)

0.051

(1.2%)

0.114

(5.1%)

0.055

(4.4%)

Unidentified 0.102

(5.0%)a

0.197

(10.7%)b

0.137

(2.6%)c

0.352

(15.9%)d

0.265

(22.2%)e

Total Characterized 2.599

(102.1%)

1.623

(87.8%)

4.605

(95.8%)

2.174

(98.1%)

1.164

(89.7%)

Uncharacterized 0.084

(3.3%)

0.327

(17.7%)

0.273

(5.7%)

0.237

(10.7%)

0.164

(12.6%)

a: 32 components, none greater than 0.009 mg/kg (0.4%TRR)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 29 of 193

b: 33 components, none greater than 0.025 mg/kg (1.3%TRR)

c: 29 components, none greater than 0.019 mg/kg (0.4%TRR)

d: 51 components, none greater than 0.069 mg/kg (3.1%TRR)

e: 45 components, none greater than 0.072 mg/kg (5.6%TRR)

ND = Not detected

Figure 3: Proposed Metabolic Pathway for Cyantraniliprole on Tomato

Rice

MRID 48122525: Rice was grown in a glasshouse under flooded conditions. Three 150 g ai/ha 

applications were made to rice via foliar spray at the 3-4 leaf stage and at 7 and 14 days after the 

initial application (BBCH 13-14 at each application). One 300 g ai/ha application was made to 

rice via soil application at the 3-4 leaf stage (BBCH 13).

Following foliar applications, TRR ranged from 0.383-2.13 ppm in foliage when sampled within 

14 days of first 3 treatments, 0.446 ppm in straw, 0.024 in grain and 0.447 ppm in roots at 

N

N N

NH

O

Cl

Br

HN

O

N

N

N

O

N

N

N

Br

Cl

N

O

H2N O

HN

NH

O

N

N N

Cl

Br

NH

O

HN

O

N

N N

Cl

Br

O

HO

N

N N

NH

O

Cl

Br

HN

O

HO

N

NH

O

N N

N

Br

Cl

N

H

O OH

N

NH

N

O

N

N

N

Br

Cl

N

OH

O

N

N

N

Br

Cl

O

H2N

N

N N

N

H

O

NH2

O

Br

Cl

NH

O

N

N

N

Br

Cl

NH2

O

N

N

N

O

N

N

N

Br

OH

N

N

N

O

N

N

H

Br

N

Cyantraniliprole

(DPX-HGW86)

IN-J9Z38

IN-JCZ38

IN-JSE76

IN-N7B69

IN-MYX98

IN-MLA84

IN-DBC80

IN-K7H19

IN-HGW87

IN-NXX70

IN-QKV54
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 30 of 193

harvest (140 days after the last application). Following one soil application, TRR ranged from 

0.40-0.42 ppm 56 days after treatment, 0.278-0.297 ppm in straw, 0.012-0.029 ppm in grain, and 

0.282-0.367 ppm in roots at harvest (175 days after treatment).

Cyantraniliprole was the major residue in rice foliage, straw, and grain. Additional minor 

metabolites include: IN-JCZ38, IN-JSE76, IN-K7H19, IN-MLA84, IN-J9Z38, IN-DBC80, INN7B69, IN-K5A77, and IN-K5A78. 

Table 8: Distribution of residues in rice following three foliar applications with [CN/PC14C]-cyantraniliprole

Tissue Foliage Strawa Grain

a

Days after foliar treatment (DAT) 0 DAT1 7 DAT1 7 DAT2 7 DAT3 14 DAT3

140 

DAT3

140 

DAT3

TRR, mg/kg 2.126 0.383 1.000 1.560 1.207 0.446 0.024

Extracted residues 99.9 92.1 99.0 96.6 95.3 83.7 48.7

Unextracted residues 0.002

(0.1%)

0.030

(7.8%)

0.010

(1.0%)

0.053

(3.4%)

0.057

(4.7%)

0.073

(16.3%)e

0.012

(51.3%)

Extracted residues after processing and 

treatment with acid, alkali and enzymes

2.152

(101.2%)

0.357

(93.2%)

1.030

(103.0%)

1.445

(92.6%)

1.168

(96.8%)

0.437

(98.0%)e

0.010

(42.9%)

Loss during processing -0.028

(-1.3%)

-0.004

(-1.0%)

-0.040

(-4.0%)

0.062

(4.0%)

-0.018

(-1.5%)

-0.016

(-3.5%)

0.001

(5.8%)

Cyantraniliprole 2.127

(100.0%)

0.347

(90.7%)

0.955

(95.5%)

1.179

(75.6%)

0.979

(81.1%)

0.109

(24.4%)

0.005

(20.9%)

IN-JCZ38 ND ND ND 0.009

(0.6%)

0.009

(0.8%)

0.042

(9.4%) ND

IN-JSE76 ND ND ND ND 0.002

(0.2%)

0.040

(9.0%) ND

IN-K7H19 ND ND ND ND ND 0.011

(2.4%) ND

IN-MLA84 ND ND 0.006

(0.6%)

0.024

(1.5%)

0.023

(1.9%) ND ND

IN-J9Z38 0.014

(0.6%)

0.010

(2.5%)

0.063

(6.3%)

0.112

(7.2%)

0.131

(10.9%)

0.018

(4.0%)

<0.001

(1.5%)

IN-DBC80 ND ND ND ND ND 0.014

(3.2%) ND

IN-N7B69 ND ND ND 0.027

(1.7%) ND ND 0.001(2.2%)

IN-K5A77 0.012

(0.6%) ND ND ND 0.010(0.8%)

0.024

(5.3%) ND

IN-K5A78 ND ND ND 0.012

(0.8%)

0.005

(0.2%) ND 0.001(2.6%)

Unidentified ND ND 0.005

(0.5%)

0.084b

(5.3%)b

0.011

(0.9%)

0.131c

(29.4%)c

0.003d

(15.7%)d

Total identified and characterized 2.152

(101.2%)

0.357

(93.2%)

1.029

(102.9%)

1.446

(92.7%)

1.168

(96.8%)

0.388

(87.1%)

0.010

(42.9%)

Uncharacterized <0.001 <0.001 0.001 -0.002

(-0.1%) <0.001 0.049(10.9%) <0.001

a: Final harvest

b: 8 components, none greater than 0.035 mg/kg (2.3%)

c: 7 components, none greater than 0.036 mg/kg (8.1%)

d: 17 components, none greater than 0.002 mg/kg (8%)

e: 10.9% of the TRR was further extracted with enzymes (1.2%) , acid (3.2%) and alkali treatment (8.4%)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Figure 4: Proposed Metabolic Pathway for Cyantraniliprole on Rice

Conclusions. The submitted plant metabolism studies are adequate. The results of the submitted 

studies with cotton, lettuce, tomato and rice indicate that the metabolism of cyantraniliprole is

similar in these crops. Overall, total radioactive residues were greater following foliar treatment. 

Cyantraniliprole was the principal residue in most crop fractions at various sampling points up to 

crop maturity. Numerous metabolites were detected in crop matrices. The metabolism of 

cyantraniliprole in plants appears to proceed via loss of water leading to the formation of 

quinazolinone ring, oxidation of the cyano group, and hydroxylation of the N-methyl and/or tolyl 

methyl group.

The submitted studies are adequate to satisfy plant metabolism data requirements. It should be 

pointed out that use of a mixture of two radiolabeled substances for foliar applications as a single 

formulation is acceptable since cleaved metabolites such as IN-DBC80 and IN-QKV54 were 

N

N N

NH

O

Cl

Br

N

O

N

N

N

O

N

N

N

Br

Cl

N

O

H2N O

N

NH

O

N

N N

Cl

Br

NH

O

N

O

N

N N

Cl

Br

O

HO

N

N N

NH

O

Cl

Br

N

O

HO

N

NH

O

N N

N

Br

Cl

N

H

O OH

N

NH

N

O

N

N N

Br

Cl

N

OH

O

N N

N

Br

Cl

O

H2N

N

N N

N

H

O

NH2

O

Br

Cl

O

HO N

N

O

N

N N

Br

Cl

O

H2N N

N

O

N

N N

Br

Cl

N

N

O

N

N

N

Br

OH

N

Cyantraniliprole

(DPX-HGW86)

IN-J9Z38

IN-JCZ38

IN-JSE76

IN-N7B69

IN-MLA84

IN-DBC80

IN-K7H19

IN-K5A77

IN-K5A78

IN-NXX70
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 32 of 193

found in low concentrations in some plant studies. Even after doubling the concentration of INDBC80 to account for the mixed label, the change in concentration is insignificant. IN-QKV54 

was a low level metabolite found primarily in immature plant samples and thus considered to be 

insignificant as a metabolite. It is also noted that the samples collected at harvest from the 

metabolism studies were substantially longer (125 days after last application) than the proposed 

treatment-to-harvest intervals (7 days or less). A different metabolic pathway on fruits and grain 

could not be excluded. However, immature and mature samples of four different crop groups 

covering a wide range of agro-climatic conditions were taken that showed very similar metabolic 

pathways. The formation of a new metabolite soon after application would be very unlikely, and 

even so, it would be very low.

The residue of concern in plant commodities for tolerance enforcement and risk assessment is 

cyantraniliprole (ROCKS decision memo, D404411, 9/25/2012).

860.1300 Nature of the Residue - Livestock

Monograph for Cyantraniliprole, Sections B.7.2.1 and B.7.2.2, B.7.2.5 (MRIDs 48119991-48119992)

There are several livestock feedstuffs associated with the proposed uses of cyantraniliprole. The 

petitioner provided livestock metabolism studies with laying hens and lactating goats, which are 

summarized below. 

Hen

MRID 48119992: In a metabolism study with [CN14C] - or [PC14C]-cyantraniliprole, 5 hens

per radiolabel and 2 concurrent controls were orally dosed for 14 consecutive days in 24 h 

intervals with 1.07 mg per kg body weight per day (corresponding to 12.8-21.3 mg ai/kg feed or 

10 ppm in the diet) and sacrificed about 23 hours after the last dose. The dosing level 

corresponds to ~300x the estimated dietary burden of cyantraniliprole to poultry (see Table 8). 

Total radioactivity was measured in the excreta and eggs, which were collected once or twice 

daily, as well as in the dissected tissues muscle, fat, liver, and skin collected at sacrifice. The 

excreta, eggs (white, yolk) and liver were analyzed for parent compound and metabolites. 

The overall recovery (sum of radioactivity in the excreta, eggs as well as organs and tissues) was 

101-103% of the administered dose. The majority of the radioactivity (97.0-99.7% of the total 

dose) was detected in the excreta collected before sacrifice. Radioactivity in eggs accounted for 

0.61-0.47% of the total dose. At sacrifice, the residues in the edible organs and tissues dissected 

from the bodies amounted to 0.66-0.54% of the total dose. From this, the bulk of the residue was 

detected in egg white.

For analysis of parent compound and metabolites, Day 1-14 egg white and egg yolk, liver, and 

Day 1-14 excreta samples from all animals were respectively pooled, and the pooled samples 

were extracted with 9:1 v/v CAN (acetonitrile):water. The resulting extracts of eggs, liver, and 

excreta were combined and the radioactive content was determined by LSC of duplicate aliquots.

The post extracted solids (PES) of liver samples were further treated with protease in pH 7 buffer 

at 37C for 24 hours before LSC analysis. Extracts were analyzed using reversed phase HPLC 

equipped with an UV detector at 254 nm. Identification of residues was by comparison of HPLC 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 33 of 193

retentions times with those of authentic reference standards. Confirmation of metabolites was by 

LC/MS/MS.

The major component in egg white and egg yolk was cyantraniliprole, at 32.5-41.7% and 10.3-

9.33% TRR, respectively; no parent was detected in liver. Other metabolites identified in eggs 

were IN-HGW87, IN-J9Z38, IN-MLA84, IN-MYX98, IN-NBC94, IN-JSE76, IN-K5A77, and 

IN-K5A79; in liver were IN-J9Z38, IN-MLA84, IN-MYX98, IN-NBC94, IN-K5A78, INK5A79, and IN-N7B69. In eggs, IN-HGW87, IN-J9Z38, and IN-MLA84 were present at >10%. 

In liver, all the metabolites were present at <0.01 ppm. Unchanged cyantraniliprole was the 

major residue (68-77%) found in excreta.

Table 9. Distribution of residues (mg/kg parent equivalents and % TRR) in eggs and liver from hens dosed with 

[CN14C]-cyantraniliprole

Residue

Egg white

(Day 1-14 

Composite)

Egg yolk 

(Day 1-14 

Composite)

Liver

Aqueous ACN 

extract Protease digest

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

Total 100.0 0.259 100.0 0.092 100.0 0.137 100.0 0.137

Total extracted 99.01 0.256 79.30 0.073 17.07 0.023 38.09 0.052

Cyantraniliprole 32.48 0.084 10.28 0.009 ND ND

IN-HGW87 0.61 0.002 11.98 0.011 ND ND

IN-J9Z38 29.21 0.075 7.42 0.006 2.08 0.003 ND

IN-MLA84 18.66 0.049 11.56 0.011 0.89 0.001 ND

IN-MYX98 6.40 0.017 5.42 0.006 0.42 0.001 1.10 0.002

IN-NBC94 0.96 0.002 0.86 0.001 ND 0.40 0.001

Total characterized unknownsa7.75 0.021 23.62 0.025 7.58 0.010 22.33 0.042

Total characterized/

identifiedb 96.07 0.250 71.14 0.068 10.97 0.015 23.82 0.045

Total uncharacterized/ 

unidentifiedc 2.94 0.008 8.16 0.005 6.10 0.008 16.94 0.023

Total unextracted 0.99 0.003 20.70 0.019 82.93 0.114 44.97 0.062

a: Consists of HPLC peaks that do not match the retention time of any known reference standard

b: Total characterized/identified = Sum of identified metabolites + Total characterized unknowns

c: Total uncharacterized/unidentified = Total extracted – Total characterized/identified

ND = Not Detected

Table 10. Concentration of residues (mg/kg parent equivalents and % TRR) in, eggs and liver from hens dosed with 

[PC14C]-cyantraniliprole

Residue

Egg white

Day 1-14 

composite

Egg yolk

Day 1-14 

composite

Liver

% TRR mg/kg % TRR mg/kg

Aqueous ACN 

extract Protease digest

% TRR mg/kg % 

TRR mg/kg

Total 100.0 0.203 100.0 0.087 100.0 0.174 100.0 0.174

Total extracted 99.7 0.202 83.1 0.072 23.0 0.040 37.6 0.065

Cyantraniliprole 41.7 0.085 9.33 0.008 ND ND

IN-HGW87 0.74 0.001 ND ND ND

IN-J9Z38 17.1 0.034 13.1 0.011 ND ND

IN-JSE76 ND 1.90 0.002 ND ND

IN-JCZ38 ND ND ND 0.23 <0.001

IN-K5A77 ND 6.19 0.005 ND ND

IN-K5A78 ND ND 0.23 <0.001 ND
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Page 34 of 193

IN-K5A79 3.90 0.007 1.52 0.001 0.27 <0.001 0.45 0.001

IN-K7H19 ND ND ND 0.46 <0.001

IN-MLA84 18.2 0.037 16.8 0.015 3.98 0.007 ND

IN-MYX98 8.13 0.016 2.09 0.002 0.27 <0.001 ND

IN-N7B69 ND ND ND 0.55 0.001

IN-NBC94 2.86 0.005 1.24 0.001 ND ND

Total characterized 

unknownsa 0.98 0.002 17.4 0.015 10.5 0.020 25.0 0.045

Total characterized/ 

identifiedb 96.2 93.6 0.187 69.6 0.060 15.2 0.027 26.7 0.047

Total 

uncharacterized 

/unidentifiedc

0.00 6.09 0.014 13.5 0.012 7.8 0.013 10.9 0.018

Total unextracted 3.84 0.33 0.001 16.9 0.015 77.0 0.134 39.5 0.053

a: Consists of HPLC peaks that do not match the retention time of any known reference standard

b: Total characterized/Identified = Sum of identified metabolites + Total characterized unknowns

c: Total uncharacterized/unidentified = Total extracted – Total characterized/identified

ND = Not Detected

Figure 5: Proposed Metabolic Pathway for Cyantraniliprole in Egg-Laying Hens

N

N N

NH

O

Cl

Br

HN

O

N

N

N

O

N

N

N

Br

Cl

N

O

H2N O

HN

NH

O

N

N N

Cl

Br

NH

O

HN

O

N

N N

Cl

Br

O

HO

N

N N

NH

O

Cl

Br

HN

O

HO

N

NH

O

N

N

N

Br

Cl

N

H

O OH

N

OH

O

N

N

N

Br

Cl

O

H2N

N

N N

N

H

O

NH2

O

Br

Cl

O

HO N

N

O

N

N N

Br

Cl

O

H2N N

N

O

N

N N

Br

Cl

NH

O

N

N

N

Br

Cl

NH2

O

N

O

HO

N

N

N

N

H

O

NH2

O

Br

Cl

N

N

O

N

N N

Br

Cl

N

HO

NH

N

O

N

N N

Br

Cl

N

Cyantraniliprole

(DPX-HGW86)

IN-J9Z38

IN-JCZ38

IN-JSE76

IN-N7B69

IN-MYX98

IN-MLA84

IN-DBC80

IN-K7H19 IN-K5A79

IN-K5A77

IN-K5A78

IN-HGW87

IN-NBC94
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 35 of 193

Conclusions - Hen. The submitted hen metabolism studies are adequate. The majority (97-

100%) of the dose was excreted. Unchanged parent was the major residue in excreta. Transfer of 

residues to eggs, fat, muscle, and liver was low. Residues in fat and muscle were <0.01 ppm. 

Unchanged cyantraniliprole was the major residue in egg white and egg yolk. Other residues 

found in eggs include IN-J9Z38 and IN-MLA84. Parent was not detected in the liver and a 

number of metabolites formed instead. These metabolites resulted from cyclization, hydrolysis of 

the cyano group, hydroxylation of the benzilic and N-methyl carbons, and cleavage at the 

pyrazole carbonyl.

Goat

MRID 48119991: A goat was orally dosed for 7 consecutive days, at 24 h intervals, with 0.44

mg [CN14C]- or [PC14C ]-cyantraniliprole per kg body weight per day (corresponding to ~35

mg ai/kg feed or 10 ppm in the diet) and sacrificed at about 24 hours after the last dose. The 

dosing level corresponds to ~24x the estimated dietary burden of cyantraniliprole to beef cattle 

and dairy cattle (see Table 8). Total radioactivity was measured in feces, urine, bile, milk, liver, 

kidney, muscle, and fat (omental, renal, subcutaneous). The milk, liver, kidney, muscle and fat 

were analyzed for parent compound and metabolites. 

The overall recovery (sum of radioactivity in the excreta, milk as well as organs and tissues) was 

96-97% of the total administered dose. As percentages of the total administered dose, 

radioactive residues were 84-88% in feces, 6.7-6.9% in urine, and 1.4-2.3% in cage wash. 

In tissues, TRR were highest in liver, at 0.46-0.495 ppm. The TRR for the other organs and 

tissues, were 0.117-0.177 ppm in kidney, 0.080-0.147 ppm in milk, 0.045-0.114 ppm in fat, and 

0.020-0.043 ppm in muscle. In total, the residues in all tissue samples were estimated to be 

about 2.0% of the total dose.

The major component in all the edible matrices was cyantraniliprole (17.1% to 49.6% TRR). 

Other metabolites identified at >10% TRR were IN-N7B69, IN-MYX98, and IN-J9Z38 (fat). 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 36 of 193

Table 11.: Distribution of radioactive residues in goat milk and tissues following administration of [CN14C]-cyantraniliprole

Component

Milka Liver Kidney Muscle Fatb

%TRR mg/kg Solvent Extract Protease Digest %TRR mg/kg %TRR mg/kg %TRR mg/kg %TRR mg/kg % TRR mg/kg

TRR 100.0 0.076 100.0 0.430 100.0 0.430 100.0 0.136 100. 0.027 100. 0.052

Extracted 99.3 0.075 54.3 0.233 26.8 0.115 62.7 0.085 61.0 0.016 90.4 0.047

Cyantraniliprole 39.5 0.030 17.1 0.073 0.3 0.002 12.7 0.017 30.3 0.009 30.8 0.016

IN-K7H19 2.6 0.001 1.0 0.004 0.9 0.005 3.0 0.004 4.6 0.001 1.9 <0.001

IN-JCZ38 7.2 0.005 0.83 0.004 0.4 0.003 ND ND ND

IN-N7B69 11.8 0.008 0.64 0.002 1.8 0.008 ND ND 1.9 <0.001

IN-K5A79 ND 0.61 0.003 ND ND ND 1.9 <0.001

IN-MYX98 15.1 0.011 2.5 0.011 0.1 <0.001 7.1 0.010 ND 1.9 <0.001

IN-JSE76 ND ND ND ND ND ND

IN-HGW87 1.1 <0.001 1.1 0.004 0.1 0.001 0.61 0.001 ND ND

IN-K5A77 ND 5.4 0.023 ND ND ND ND

IN-NBC94 ND ND ND ND ND 1.9 <0.001

IN-K5A78 ND 0.83 0.003 0.2 0.001 ND ND 1.9 <0.001

IN-MLA84 ND 3.7 0.017 0.2 0.001 4.1 0.005 ND 1.9 0.001

IN-J9Z38 0.48 <0.001 ND ND 1.1 0.001 ND 26.9 0.014

Unknowns 12.7 0.006 13.2c

0.053c18.6ed 0.080d13.2 0.018 7.6 0.003 11.5 0.006

Total Char./Ident. 90.5 0.061 46.9 0.202 22.6 0.101 41.8 0.056 42.5 0.013 75.0 0.039

Total Unextracted 0.7 0.001 45.7 0.197 18.9e

0.082e37.4 0.051 39.2 0.011 9.6 0.005

a: Day 1-7 Composite Sample

b: Concentrations are a mean of omental, renal, and subcutaneous fat samples analyzed.

d: 13 components, none greater than 0.006 mg/kg (1.4%TRR)

e: 32 components, none greater than 0.006 mg/kg (1.5%TRR)

f: Terminal unextracted residues following protease digestion

ND = Not Detected.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 37 of 193

Table 12.: Distribution of radioactive residues in goat milk and tissues following administration of [PC14C]-cyantraniliprole

Component

Milka Liver Kidney Muscle Fatb

%

TRR mg/kg Solvent Extract Protease Digest %TRR mg/kg %TRR mg/kg %TRR mg/kg % TRR mg/kg % TRR mg/kg

TRR 100. 0.140 100.0 0.500 100.0 0.500 100.0 0.206 100.0 0.040 100.0 0.119

Extracted 99.0 0.139 59.5 0.296 20.7 0.103 78.6 0.162 80.9 0.032 98.3 0.117

Cyantraniliprole 49.6 0.070 27.3 0.136 ND 18.9 0.040 15.3 0.006 45.4 0.054

IN-K7H19 0.57 0.001 0.95 0.005 0.5 0.003 2.3 0.004 ND <0.8 <0.001

IN-JCZ38 1.32 0.002 0.42 0.002 ND 0.64 0.001 ND ND

IN-DBC80 ND ND ND ND ND <0.8 <0.001

IN-N7B69 2.01 0.003 0.32 0.002 ND 1.7 0.003 ND <0.8 <0.001

IN-K5A79 ND 0.61 0.003 ND ND ND 2.5 <0.003

IN-MYX98 18.3 0.026 3.6 0.017 ND 7.1 0.014 32.8 0.014 <0.8 <0.001

IN-JSE76 ND ND ND ND ND ND

IN-HGW87 0.69 0.001 1.0 0.005 ND 1.1 0.002 ND ND

IN-K5A77 ND 5.7 0.028 ND 0.70 0.001 4.4 0.001 <0.8 <0.001

IN-NBC94 ND ND ND ND ND 1.7 0.002

IN-K5A78 1.26 0.002 3.4 0.017 0.3 0.002 ND ND <0.8 <0.001

IN-MLA84 2.24 0.003 5.3 0.026 0.4 0.002 1.8 0.004 ND 1.7 0.002

IN-J9Z38 3.72 0.005 0.9 0.004 ND ND 1.1 <0.001 24.4 0.029

Unknowns 8.81 0.012 6.2c0.031c11.6d0.059d36.9e0.076e17.1 0.006 5.0 0.006

Total Charac./Ident. 88.4 0.125 55.7 0.279 12.8 0.066 71.0 0.145 70.9 0.027 79.8 0.095

Total Unextracted 0.83 0.001 40.5 0.204 19.9f0.101 21.5 0.044 19.2 0.008 1.7 0.002

a Day 1-7 Composite Sample

b Concentrations are a mean of omental, renal and subcutaneous fat samples analyzed

c 7 components, none greater than 0.008 mg/kg (1.6%TRR)

d 19 components, none greater than 0.009 mg/kg (1.8%TRR)

e 10 components, none greater than 0.023 mg/kg (11.4%TRR)

f Terminal unextracted residues following protease digestion

ND = Not Detected
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Figure 6: Proposed Metabolic Pathway of Cyantranilirprole in Lactating Goats

Conclusions - Goat. The submitted goat metabolism studies are adequate to satisfy data 

requirements. Cyantraniliprole residues were rapidly eliminated by the lactating goat primarily 

in the excreta (94-96%). In feces, unchanged cyantraniliprole was the major residue accounting

for 67-71% of the dose. In urine, cyantraniliprole was extensively metabolized and represented 

no greater than 0.5% of the dose. Transfer of residues to edible tissues was low at 1-2% of the 

dose. Also, about 1-2% of the dose was eliminated through the milk. The major residue in milk, 

liver, kidney, muscle and fat was cyantraniliprole. About a dozen metabolites were detected at 

difference levels in different tissues. The formation of these metabolites indicate a variety of 

chemical reactions similar to those found in hens, such as cyclization, hydrolysis of the cyano 

group, hydroxylation of the benzilic and N-methyl carbons, and cleavage of the pyrazole 

carbonyl. 

N

N N

NH

O

Cl

Br

HN

O

N

N

N

O

N

N

N

Br

Cl

N

O

H2N O

HN

NH

O

N

N N

Cl

Br

NH

O

HN

O

N

N N

Cl

Br

O

HO

N

N N

NH

O

Cl

Br

HN

O

HO

N

NH

O

N

N

N

Br

Cl

N

H

O OH

N

NH

N

O

N

N N

Br

Cl

N

OH

O

N

N

N

Br

Cl

O

H2N

N

N N

N

H

O

NH2

O

Br

Cl

O

HO N

N

O

N

N N

Br

Cl

O

H2N N

N

O

N

N N

Br

Cl

NH

O

N

N

N

Br

Cl

NH2

O

N

O

HO

N

N

N

N

H

O

NH2

O

Br

Cl

N

N

O

N

N N

Br

Cl

N

HO

Cyantraniliprole

(DPX-HGW86)

IN-J9Z38

IN-JCZ38

IN-JSE76

IN-N7B69

IN-MYX98

IN-MLA84

IN-DBC80

IN-K7H19

IN-K5A79

IN-K5A77

IN-K5A78

IN-HGW87

IN-NBC94
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Overall Conclusions - Livestock Metabolism. The nature of the residue in livestock is adequately 

understood. The metabolism of cyantraniliprole in poultry and ruminants was found to be 

similar. The residue of concern for tolerance enforcement is cyantraniliprole, and the residues of 

concern for risk assessment include cyantraniliprole and its metabolites IN-J9Z38, IN-N7B69, 

IN-MLA84, and IN-MYX98 (ROCKS decision memo, D404411, 9/25/2012). 

860.1340 Residue Analytical Methods

Monograph for Cyantraniliprole, Section B.5.2 (MRIDs 48119922, 48119923, 48119924, 48119925, 48119928, 

48119929, 48119932, 48119933, 48119934, 48119998, 48119999, 48120001, 48122526, 48122534)

Analytical methods are available for tolerance enforcement in plants and processed commodities, 

and livestock commodities.

DuPont -18846, method for the analysis of cyantraniliprole and its metabolites (IN-N7B69, INJCZ38, IN-K7H19, IN-MYX98, IN-MLA84 and IN-J9Z38) in various crops with LC/MS/MS 

detection, was validated in grapes, apples, peaches, tomatoes, almonds (nut meal), lettuce, wheat 

grain, wheat straw, potatoes, lemons, rape seed, tomato paste, and sun-dried tomato with an LOQ 

of 0.01 ppm cyantraniliprole. 

Briefly, A 10 g crop sample is extracted twice with 20 mL of a 90:10 mixture of 

acetonitrile:water solution by shaking at high speed on a Geno Grinder Shaker. Ball bearings are 

present during the extraction to aid the effectively extracting cyantraniliprole and metabolites INJ9Z38, IN-JCZ38, IN-K5A79, IN-K7H19, IN-MLA84, IN-MYX98, and IN-N7B69 from the 

plant material. The samples were shaken at 700 cycles per minute on a Geno Grinder Shaker for 

2 minutes. The extract was then centrifuged at 3000 RPM for 5 minutes. Next, the supernatant 

was decanted into a clean 50-mL centrifuge tube. An additional 20 mL of acetonitrile:water 

extraction solution was added to the sample pellet, shaken at 700 cycles per minute on a Geno 

Grinder Shaker for 2 minutes, then the extract was centrifuged at 3000 RPM for 5 minutes. The 

supernatant was combined into the same 50-mL centrifuge tube used previously in the process. 

The extract was diluted to 50 mL using acetonitrile:water extraction solution and vigorously mix 

the extract. Approximately 1 mL of extract was filtered with a 3-mL syringe and a 0.2-μm PTFE 

filter into a 13-mL centrifuge tube. Then, 0.10 mL of the filtered extract was pipetted into a 

HPLC vial and an additional 0.40 mL of methanol and 0.50 mL of de-ionized water solution 

were pipetted and mixed well. Samples were then analyzed using LC-MS/MS (ESI MRM 

positive mode). IN-MYX98 was also quantified in wheat using the negative mode. 

The following mass transitions are used for qualitative identification and quantification.

Analyte Mass transition 1 Mass transition 2

Cyantraniliprole 475 → 444 475 → 286

IN-J9Z38 457 → 188 457 → 299

IN-JCZ38 493 → 286 493 → 462
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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INK7H19 479 → 286 479 → 462

IN-MLA84 443 → 298 443 → 407

IN-MYX98 491 → 444

489 → 379 (negative mode)

491 → 473

Recoveries for all matrices except tomato paste and sun-dried tomato ranged from 78-103%, and 

from 77-94% for tomato paste and sun-dried tomato when fortified at 0.01 ppm cyantraniliprole.

DuPont-25543, analytical method for the determination of cyantraniliprole, IN-HGW87, INN7B69, IN-K7H19, IN-JCZ38, IN-MYX98, IN-J9Z38 and IN-MLA84 in bovine tissues, milk 

and eggs using LC/MS/MS, was validated with an LOQ of 0.01 ppm cyantraniliprole in muscle, 

liver, kidney, fat, milk, and egg.

Briefly, a 10 g sample is extracted twice with 20 to 25 mL of acetonitrile using a Geno-Grinder 

for 2 minutes. The two extractions are combined and brought to a volume of 50 mL using 

acetonitrile. All of the extracts were purified using the same extraction procedure. Using the 10-

mL aliquot previously extracted, 3 mL of hexane was added and shaken by hand for 1 minute. 

The sample was then centrifuged on the bench top centrifuge for 5 minutes. The sample was 

removed from the centrifuge and the upper layer of hexane removed and discarded. A sample 

was taken and put on a nitrogen evaporator until the sample was approximately 0.5 mL. The 

sample was then increased to a volume of 5 mL by adding methanol and then vortexed and 

sonicated. Then, 5 mL of water was added for a 50/50 solution, sonicated, vortexed, and filtered 

before analyzing. In cases where matrix interferences is noted, an additional purification step 

involving salt out should be included after the hexane partition. This step consists of adding 0.5 

grams of salt to the sample, followed by shaking for 1 minute by hand. The sample is then 

centrifuged for 10 minutes at 3000 RPM, and the upper layer is transferred to a new 50-mL 

centrifuge tube.

Cyantraniliprole, IN-HGW87, IN-J9Z38, IN-JCZ38, IN-K5A79, IN-K7H19, IN-N7B69, INMLA84, and IN-MYX98 were separated from co-extracts by reversed phase liquid 

chromatography (LC) and detected by mass spectrometry/mass spectrometry (MS/MS) using 

positive ion atmospheric pressure chemical ionization (APCI). The transitions used are:

Analyte Mass transition 1 Mass transition 2

Cyantraniliprole 475 → 443 475 → 286

IN-J9Z38 457 → 298 457 → 188

IN-JCZ38 493 → 462

INK7H19 479 → 462 479 → 286

IN-MLA84 443 → 298 443 → 407

IN-MYX98 491 → 444
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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IN-N7B69 491 → 286

IN-K5A79 480 → 463 480 → 286

IN-HGW87 459 → 284 459 → 112

Extraction efficiency

For plant commodities, DuPont submitted data for the enforcement method using samples of 

lettuce, beet foliage, wheat grain, and wheat hay from the metabolism studies. From 89-100% 

TRR were extracted from [

14C] cyantraniliprole-treated wheat grain, wheat hay, beet foliage and 

lettuce using the extraction procedures of the enforcement method. The extraction procedures of 

the plant and livestock commodity analytical methods are similar to those used in the metabolism 

studies except with the use of a Geno Grinder in place of Tekmar Tissue-mizer probe. Also 

submitted were validation data using samples of liver, muscle, milk, egg white, and egg yolk. 

The levels of extracted radioactivity obtained using the residue method were equivalent of those 

found in the metabolism extraction: 75.5% of liver, 92.7% for muscle, 104% for milk, 99.5% for 

egg white, and 125% for egg yolk. These data are adequate to satisfy extraction efficiency 

requirement for the plant and livestock methods. 

The methods used for data collection in the field trials and processing studies (Method 15736) 

and livestock feeding studies (Method 18844) are the same as the enforcement methods and 

were shown to be adequate via concurrent recoveries for fortified samples. See field trial 

sections for specific crops for recovery summaries.

Conclusions. The submitted residue analytical method data are adequate. Adequate validation 

data have been provided for the data collection and the proposed enforcement methods. 

Adequate ILV data have been submitted for the proposed enforcement methods. 

860.1360 Multiresidue Methods

Monograph for Cyantraniliprole, Section B.5.2.1 (MRIDs 48119928, 48119929, 48119933, 48119999, 48122526)

Cyantraniliprole has been evaluated using the multi-residue methodologies (MRM). The analysis 

of cyantraniliprole residues in crops was evaluated using US FDA protocol A through F. The 

results from this study indicate that cyantraniliprole residues cannot be analyzed using the FDA 

multi-residue methodology.

Protocol A

Some of the test substances, cyantraniliprole and metabolites IN-J9Z38, IN-JCZ38, IN-K7H19, 

IN-MLA84, IN-MYX98, and IN-N7B69, fluoresced by the methods outlined in Protocol A, but 

none were capable of being evaluated further without modifying the methods; therefore, 

recovery through the complete method was not performed.

Protocol B

The test substance was not evaluated through Protocol B because it does not possess a carboxylic 

acid or phenolic moiety.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Protocol C

IN-JCZ38-004 and IN-K7H19-001 can be chromatographed and detected by module DG 18, 

Level II (ECD with DB-225 type column). Module DG 18 was chosen as the most appropriate 

determinative step for use in Protocols D, E, and F.

Protocols D, E, and F

The test substances proved insoluble in the solvents required by the Florisil cleanup methods; 

therefore, testing was suspended.

EU Multiresidue Method

The DFG multiresidue method is adequate for determination of cyantranailiprole in plant 

matrices. 

Cyantraniliprole was extracted from the plant tissue according to extraction module E 1 (tomato), 

E 2 (wheat grain), E 3 (orange), and E 7 for (almond) of the DFG Method S 19 method. Extracts 

of tomato, orange, wheat grain, and almond were analyzed by liquid chromatography with 

electro spray ionization (MRM positive mode) and tandem mass spectrometry detection. The 

DFG Method S 19, using gas chromatography for detection could not be applied to 

cyantraniliprole as degradation of cyantraniliprole was observed under GC/MS conditions.

Recovery data from various matrix fortifications are summarized in Table 13.

Table 13. Fortification Recoveries for method DFG S19 for the determination of cyantraniliprole in 

plant commodities

Matrix

mass 

transition 

(m/z)

fortification 

level (mg/kg)

number of 

values Min (%) Max (%)

Mean 

(%)

RSD 

(%)

Tomato 475 -> 286 unfortified 1* nd

0.01 5 93 101 98.2 3.2

0.1 5 111 119 114.6 2.7

475 -> 112 unfortified 1* nd

0.01 5 90 104 99.8 5.6

0.1 5 112 119 115.2 2.2

Wheat grain 475 -> 286 unfortified 2 nd**

0.01 5 92 100 96.8 3.5

0.1 5 89 108 101.0 7.2

475 -> 112 unfortified 2 nd*

0.01 5 91 103 96.0 5.9

0.1 5 91 110 102.6 7.2

Orange 475 -> 286 unfortified 2 nd**

0.01 5 87 104 92.4 7.4

0.1 5 89 98 92.8 4.0

475 -> 112 unfortified 2 nd**

0.01 5 80 99 89.2 8.7

0.1 5 87 96 91.0 4.3
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Almond 475 -> 286 unfortified 2 nd**

0.01 5 101 107 104.2 2.9

0.1 5 96 107 102.4 4.3

475 -> 112 unfortified 2 nd**

0.01 5 100 114 106.6 5.3

0.1 5 95 110 103.2 5.5

* a blank control has been analyzed with a content of 0.0037 or 0.0040 mg/kg, this sample was contaminated and not 

representative of blank tomato. 

** value found in the samples were below the LOD of 0.002 mg/kg

Conclusions. The above data are adequate to satisfy the FDA MRM data requirements. At the 

same time, DFG S19 is adequate as a multi-residue method in quantifying residues of 

cyantraniliprole in plant commodities.

860.1380 Storage Stability

Monograph for Cyantraniliprole, Section B.7.6.2, B.7.8.2, B.7.8.1 (MRIDs 48119993, 48120014, 48120015)

The stability of cyantraniliprole and metabolites IN-F6L99, IN-J9Z38, cyantraniliprole, INF6L99, INJ9Z38, IN-JCZ38, IN-K7H, IN-MLA84, IN-JCZ38, IN-K7H, IN-MLA, IN-MYZ98, 

IN-N7B, IN-N5M, IN-MYX, IN-N7B, and IN-N5M under conditions of frozen storage were 

studied for the following commodities: apple, peanut, dried beans, potato, and grape. The results 

are summarized in Table 14.

Table 14: Summary of stability of residue components in representative plant matrices under frozen storage (≤-

18°C).

Residue 

component

High-water

content matrix

High-starch

content matrix

High-acid

content matrix

High-protein

content matrix

High-oil

content matrix

Crop Months Crop Months Crop Months Crop Months Crop Months

Cyantraniliprole Apple 24 Potato 24 Grapes 24 Dried 

beans 18 Peanuts 18

IN-F6L99 Apple 24 Potato 24 Grapes 24 Dried 

beans 18 Peanuts 18

IN-J9Z38 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts 24

IN-JCZ38 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts Not stable

IN-K7H19 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts Not stable

IN-MLA84 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts 24

IN-MYX98 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts 18

IN-N7B69 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts 12

IN-N5M09 Apple 24 Potato 24 Grapes 24 Dried 

beans 24 Peanuts 24
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Table 15: Summary of Storage Conditions and Durations for Crop Field Trials, Rotational Crop 

Field Trials, and Processing Studies

Matrix

Storage 

Temperature

(oC)

Longest Actual Storage 

Interval from Sampling 

to Extraction

Longest Actual 

Storage 

Interval from 

Extraction to 

Analysis

Interval of 

Demonstrated 

Storage 

Stability 

Days Months Days Months Months

Crop Field Trials

Potato tubers -20±10 327 10.8 84 3.0

Potatoes (high 

starch/root 

vegetable: 24 

months for all 

analytes

Dry bulb onion

-20±10

370 12.2

55 1.8

High water content 

commodities: 24 

months for all 

analytes

Green onion 220 7.2

Head/Leaf Lettuce

-20±10

167 5.5

70 2.3

High water content 

commodities: 24 

months for all 

analytes

Celery 271 8.9

Spinach 184 6.0

Broccoli/Cauliflower

-20±10

190 6.2

148 4.9

High water content 

commodities: 24 

months for all 

analytes.

Cabbage (RAC) 123 4.0

Cabbage (prepared for 

consumption) 94 3.1

Mustard Greens 238 7.8

Broccoli/Cauliflower 190 6.2

Fruiting Vegetables

(peppers, tomatoes) -20±10 236 7.8 86 2.8

High water content 

commodities: 24 

months for all 

analytes

Fruiting Vegetables

(peppers, tomatoes)

Europe

-20 240 8.0 - -

High water content 

commodities: 24 

months for all 

analytes

Cucurbit Vegetables

(melon, cucumber, 

summer squash)

-20±10 282 9.3 70 2.3

High water content 

commodities: 24 

months for all 

analytes

Citrus fruits 

(orange, grapefruit, 

lemon) -20±10

Peel/pulp:157

(for 98% of the 

samples, with 

less than 2% 

stored for 370 

days).

5.1

(for 98% of 

samples, and 

12.1 for the 

remainder)

46 1.5

High acid content 

commodities: 24 

months for all 

analytes.

Apple

-20±10

210 6.9

76 2.5

High water content 

commodity: 24 

months for all 

analytes

Pear 172 5.7

Stone fruits

(peach, plum, cherry) -20±10 206 6.8 101 3.3

High water content 

commodity: 24 

months for all 

analytes

Blueberry -20±10 114 3.7 4 0.1

High acid content 

commodity: 24 

months for all 

analytes
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 45 of 193

Table 15: Summary of Storage Conditions and Durations for Crop Field Trials, Rotational Crop 

Field Trials, and Processing Studies

Matrix

Storage 

Temperature

(oC)

Longest Actual Storage 

Interval from Sampling 

to Extraction

Longest Actual 

Storage 

Interval from 

Extraction to 

Analysis

Interval of 

Demonstrated 

Storage 

Stability 

Days Months Days Months Months

Tree nuts

(almond, pecan) -20±10 188 6.2 37 1.2

High oil content 

commodity: 24 

months for INJ9Z38, IN-MLA84, 

and IN-N5M09; 18 

months for parent, 

IN-FL6L99, and 

IN-MYX98; 12 

months for INN7B69; not stable 

for IN-JCZ38 and 

IN-K7H19.

Cotton -20±10 237 7.8 88 2.9

High oil content 

commodity: 24 

months for INJ9Z38, IN-MLA84, 

and IN-N5M09; 18 

months for parent, 

IN-FL6L99, and 

IN-MYX98; 12 

months for INN7B69; not stable 

for IN-JCZ38 and 

IN-K7H19.

Canola and Sunflower -20±10 194 6.4 112 3.7

High oil content 

commodity: 24 

months for INJ9Z38, IN-MLA84, 

and IN-N5M09; 18 

months for parent, 

IN-FL6L99, and 

IN-MYX98; 12 

months for INN7B69; not stable

for IN-JCZ38 and 

IN-K7H19.

Rotational Crop Field Trials

Lettuce, radish, oats, 

soybean -20 655 22 76 2.5

High water, starch, 

acid content: 24 

months for 

cyantraniliprole.

High protein, high 

oil content:

18 months for 

cyantraniliprole 

Turnip, sugar beet, 

garden beet, carrot, 

pea, soybean, sweet 

corn, sorghum, rice, 

wheat, Bermuda grass, 

alfalfa, bromegrass, 

-20 364 12 148 5

High water, starch, 

acid content: 24 

months for 

cyantraniliprole.

High protein, high 

oil content:
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Table 15: Summary of Storage Conditions and Durations for Crop Field Trials, Rotational Crop 

Field Trials, and Processing Studies

Matrix

Storage 

Temperature

(oC)

Longest Actual Storage 

Interval from Sampling 

to Extraction

Longest Actual 

Storage 

Interval from 

Extraction to 

Analysis

Interval of 

Demonstrated 

Storage 

Stability 

Days Months Days Months Months

clover, bluegrass, 

radish, peanut, 

strawberry

18 months for 

cyantraniliprole

Processing

Potato tubers and 

processed commodities -20±10 189 6.2 84 3.0

Potatoes (high 

starch/root 

vegetable: 24 

months for all 

analytes

Cooked Spinach

Cooking water -20±10 163104

5.4

3.4 70 2.3

High water content 

commodities: 24 

months for all 

analytes

Tomatoes Processed 

Commodities -20±10 183 6.0 16 0.5

High water content 

commodities: 24 

months for all 

analytes

Orange Processed 

Commodities -20±10 200 6.6 46 1.5

High acid content 

commodities: 24 

months for all 

analytes.

Apple processed 

commodities -20±10 155 5.1 76 2.5

High water content 

commodity: 24 

months for all 

analytes

Dried plums -20±10 101 3.3 68 2.2

High water content 

commodity: 24 

months for all 

analytes

Cotton Processed 

Commodities -20±10 148 4.9 88 2.9

High oil content 

commodity: 24 

months for INJ9Z38, IN-MLA84, 

and IN-N5M09; 18 

months for parent, 

IN-FL6L99, and 

IN-MYX98; 12 

months for INN7B69; not stable 

for IN-JCZ38 and 

IN-K7H19.

Poultry matrix samples were stored at ca. -80 C (-112 F) for a maximum of 97 days. 

Cyantraniliprole and its 7 metabolites (IN-HGW87, IN-J9Z38, IN-JCZ38, IN-K7H19, 

IN-MLA84, IN-MYX98, and IN-N7B69) were shown to be stable over a period of 99 days in 

eggs. All muscle and skin with fat samples were analyzed within 20 days. All liver samples were 

extracted on the day of collection and analyzed. Stability in tissues was not investigated, but as 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 47 of 193

all tissue samples were stored less than 30 days in feeding studies, no data are required.

Table 16. Recovery data following storage in eggs

Analyte

Time zero 99 days of frozen storage in eggs

% 

Recovery

Mean 

recovery 

(%)

Fresh % 

recovery

Mean 

fresh 

recovery 

(%)

Stored 

recovery 

(%)

Mean 

stored 

recovery 

(%)

Corrected Mean 

Recovery in 

Stored Samples 

(%)

Cyantraniliprole 100, 96 98 101, 99 100 99, 102 101 101

IN-HGW87 102, 97 99 97, 95 96 103, 105 104 108

IN-J9Z38 100, 96 98 84, 87 85 83, 85 84 99

IN-JCZ38 94, 90 92 94, 93 93 90, 92 91 98

IN-K7H19 98, 92 95 95, 95 95 94, 97 95 100

IN-MLA84 105, 100 103 72, 73 73 83, 80 81 111

IN-MYX98 107, 102 105 98, 97 97 96, 99 98 101

IN-N7B69 113, 105 109 82, 81 81 77, 80 79 98

Ruminant matrix samples were stored at ca. -80 C (-112 F) for a maximum of 42 days. 

Cyantraniliprole and its 7 metabolites (IN-HGW87, IN-J9Z38, IN-JCZ38, IN-K7H19, 

IN-MLA84, IN-MYX98, and IN-N7B69) were shown to be stable over a period of 99 days in 

milk. All muscle and fat samples were analyzed within 24 days. All liver and kidney samples 

were extracted on the day of collection and analyzed. Stability in tissues was not investigated, 

but as all samples were stored less than 30 days in feeding studies, no data are required.

Table 17: Recovery data following storage in milk

Analyte

Time zero 99 days of frozen storage in milk

% 

Recovery

Mean 

recovery 

(%)

Fresh % 

recovery

Mean 

fresh 

recovery 

(%)

Stored 

recovery 

(%)

Mean stored 

recovery (%)

Corrected Mean 

Recoveries in 

Stored Samples 

(%)

Cyantraniliprole 93, 99 96 104, 95 100 94, 104 99 99

IN-HGW87 99, 105 102 112, 103 108 106, 119 113 105

IN-J9Z38 90, 98 94 85, 69 77 64, 73 69 90

IN-JCZ38 89, 92 90 99, 92 96 85, 100 93 97

IN-K7H19 96, 100 98 105, 95 100 92, 106 99 99

IN-MLA84 90, 97 93 66, 50 58 47, 66 56 97

IN-MYX98 101, 105 103 101, 92 96 91, 104 98 102

IN-N7B69 113, 119 116 97, 88 93 80, 94 87 94

Conclusions. 

Cyantraniliprole was stable (70 – 120% recovery) for 24 months in high water content plant 

matrices, high starch content matrices, and high acid content matrices and was stable for 18 

months in high protein content matrices and high oil content matrices. A similar stability pattern 

was observed for the metabolites, except IN-JCZ38 and IN-K7H19 were not stable in a high oil 

content matrix. These results cover the five major types of matrices and may be extrapolated to 

all plant commodity primary and processed matrices. These intervals of demonstrated storage 

stability cover the storage intervals of samples from the primary crop field trials, rotational crop 

field trials, and processing studies. No corrections need be applied to the residue study results.

Cyantraniliprole and seven metabolites were stable (70 – 120% recovery) for 99 days in egg 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 48 of 193

samples and milk samples stored frozen at -80 C. This interval is longer than the storage 

intervals for egg and milk samples from the feeding studies, and storage stability has been 

demonstrated. As tissue samples were analyzed immediately or within 30 days, storage stability 

data are not required, and no correction of residue values from livestock commodities are 

needed. 

860.1400 Water, Fish, and Irrigated Crops

There are no proposed uses that are relevant to this guideline topic.

860.1460 Food Handling

DuPont has proposed use of HG-W86-SC, an 18.66% or 1.67 lb ai/gallon suspension concentrate 

formulation for use in food handling establishments. This includes use when food/feed is

present. No studies were submitted in support of the use. 

Conclusion:

Cyantraniliprole may not be used in food and feed handling establishments, pending submission 

and evaluation of the appropriate studies per guideline 860.1460.

860.1480 Meat, Milk, Poultry, and Eggs

Monograph for Cyantraniliprole, Sections B.7.8.1 and B.7.8.2 (MRIDs 48120014 and 48120015)

There are livestock feedstuffs associated with the proposed uses of cyantraniliprole, and the 

following ones are considered important and represent maximum dietary burdens: almond hulls, 

cotton gin byproducts, cotton meal, field corn grain (rotation), grass hay (rotation), potato culls, 

soybean hay (rotation), and sunflower meal. As a result, the estimated dietary burdens of 

cyantraniliprole to livestock, as presented in Table 9, are conservative. The estimated dietary 

burdens are 0.37 ppm for beef cattle, 0.41 ppm for dairy cattle, 0.01 ppm for poultry, and 0.011

ppm for swine. The submitted feeding studies with cattle and poultry are summarized below.

Table18. Calculation of Dietary Burdens of Cyantraniliprole Residues to Livestock.

Feedstuff Type1 % Dry 

Matter2

% Diet2 Expected Residue Dietary Contribution 

(ppm) 3

Value used ppm

Beef Cattle: 15% R, 80% CC, 5% PC

Cotton, gin byproducts R 90 5 Median 3.15 0.175

Grass, hay (rotation) R 90 10 HAFT 0.23 0.026

Potato, culls CC 20 30 HAFT 0.11 0.165

Corn, field, grain 

(rotation)

CC 88 50 Median <0.01 0.005

Cotton, meal PC 89 5 PF x Median 0.016 0.000

TOTAL BURDEN -- -- 100 -- -- 0.37

Dairy Cattle: 45% R, 45% CC, 10% PC

Almond, hulls R 90 10 Median 1.60 0.178

Soybean, hay (rotation) R 89 20 HAFT 0.63 0.142

Grass, hay (rotation) R 90 15 HAFT 0.23 0.038
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Table18. Calculation of Dietary Burdens of Cyantraniliprole Residues to Livestock.

Feedstuff Type1 % Dry 

Matter2

% Diet2 Expected Residue Dietary Contribution 

(ppm) 3

Value used ppm

Potato, culls CC 20 10 HAFT 0.11 0.055

Corn, field, grain 

(rotation)

CC 88 35 Median <0.01 0.004

Cotton, meal PC 89 10 PF x Median 0.016 0.0018

TOTAL BURDEN -- -- 100 -- -- 0.42

Poultry: 75% CC, 25% PC

Corn, field, grain 

(rotation)

CC 88 75 Median <0.01 0.007

Cotton, meal PC 89 20 PF x Median 0.016 0.003

Sunflower, meal PC 92 5 PF x Median 0.007 0.000

TOTAL BURDEN -- -- 100 -- 0.01

Swine: 85% CC, 15% PC

Corn, field, grain 

(rotation)

CC 88 85 Median <0.01 0.0085

Cotton, meal PC 89 15 PF x Median 0.016 0.0024

TOTAL BURDEN -- -- 100 -- 0.011

1

 R: Roughage; CC: Carbohydrate concentrate; PC: Protein concentrate.

2

 OCSPP 860.1000 Table 1 Feedstuffs (June 2008). 

3

 Contribution = ([expected residue /% DM] X % diet) for beef and dairy cattle; contribution = ([expected residue] 

X % diet) for swine and poultry.

Cattle

MRID 48120014: Four treatment groups of three dairy cows each were dosed orally in gelatin 

capsules (twice daily) for 28 consecutive days with cyantraniliprole at 3, 10, 30, and 100 ppm in 

the animal diet on a dry weight basis. Two cows were not dosed to serve as controls. An 

additional 3 cows were dosed at 100 ppm for 28 days to obtain depuration data. The dosing 

levels of the groups represent 8.1x, 27x, 81x, and 270x, respectively, the dietary burden to beef 

cattle and 7.3x, 24x, 73x, and 244x, respectively, the dietary burden to dairy cattle. 

Cows were milked twice daily and samples from the afternoon samplings were combined with 

samples from the next morning. Milk samples were collected from individual cows daily and 

milk collected on Days -1, 1,3, 5, 10, 14, 21, 28 were analyzed. Residues in milk reached a 

plateau after approximately of 5 days and did not appear to accumulate in milk. Cows (except for 

the depuration group) were sacrificed less than 24 hours after the final dose. Samples of liver, 

muscle (loin and flank or round leg), kidney, and fat (omental, perirenal, and subcutaneous) were 

taken for analysis. Samples of cream (milk fat) and skim milk were generated from milk 

samples collected on study Day 14 and 21. 

Samples of cattle (and poultry) matrices were analyzed for residues of cyantraniliprole and seven 

of its metabolites using HPLC/MS/MS (DuPont-18844). Residues were expressed in terms of 

each analyte. This method was adequate for data collection based on acceptable method 

recoveries: 80-102% for parent, 79-100% for IN-HGW87, 84-94% for IN-J9Z38, 80-94% for 

IN-JCZ38, 87-101% for IN-K7H19, 80-97% for IN-MLA84, 84-105% for IN-MYX98, and 87-
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98% for IN-N7B69. The validated LOQs in each matrix were 0.01 ppm for cyantraniliprole and 

metabolites. 

Milk and tissue samples were stored frozen (≤-80 oC) for a maximum of 42 days. Freezer storage 

stability data demonstrated that residues of cyantraniliprole and metabolites are stable in fortified 

samples of milk at -80 oC for up to 99 days. All muscle and fat samples were analyzed within 24 

days, and all liver and kidney samples were extracted on the day of collection and analyzed. 

The results of the cattle feeding study are summarized in Table 19. Residues were highest in 

liver, followed by kidney, fat and muscle. Residue levels in tissues were dose dependent.

In whole milk, the maximum average cyantraniliprole residues were 0.030, 0.11, 0.25, and 0.71 

ppm, respectively, in the 3, 10, 30 and 100 ppm dose groups. The metabolite that had the highest 

residues in milk was IN-N7B69, present at 0.028, 0.074, 0.17 and 0.28 ppm, respectively. 

Residue levels in cream were higher than in skim milk. 

Table 19. Residues of cyantraniliprole and metabolites in milk products from lactating cows 

dosed with cyantraniliprole for 28 days

Matrix Analyte

Values in milk represent the highest pm + am pooled sample value

3 mg/kg 10 mg/kg 30 mg/kg 100 mg/kg

Maximum Residue (Day 5 – 28) ppm

Milk

Cyantraniliprol

e

0.030 0.11 0.25 0.71

IN-HGW87 ND ND <0.01 <0.01

IN-J9Z38 <0.01 <0.01 0.010 0.034

IN-JCZ38 ND ND ND ND

IN-K7H19 ND ND ND ND

IN-MLA84 <0.01 <0.01 <0.01 <0.01

IN-MYX98 <0.01 <0.01 0.025 0.085

IN-N7B69 0.028 0.074 0.17 0.28

Study day (100 mg/kg) 14 21 14 21 14 21 14 21

Cream

Cyantraniliprol

e

0.072 0.059 0.2 0.15 0.63 0.46 1.9 1.7

IN-HGW87 ND ND ND ND <0.01 <0.01 <0.01 <0.01

IN-J9Z38 0.014 0.011 0.032 0.027 0.085 0.066 0.37 0.31

IN-JCZ38 ND ND ND ND ND ND ND ND

IN-K7H19 ND ND ND ND ND ND ND ND

IN-MLA84 <0.01 <0.01 <0.01 <0.01 0.026 0.021 0.039 0.041

IN-MYX98 <0.01 <0.01 <0.01 <0.01 0.020 0.023 0.066 0.079

IN-N7B69 0.019 0.022 0.048 0.053 0.12 0.14 0.19 0.25

Skim milk

Cyantraniliprol

e

0.019 0.014 0.049 0.039 0.15 0.13 0.47 0.47

IN-HGW87 ND ND ND ND ND ND <0.01 <0.01

IN-J9Z38 ND ND ND ND ND <0.01 <0.01 <0.01

IN-JCZ38 ND ND ND ND ND ND 0.030 ND

IN-K7H19 ND ND ND ND ND ND ND ND

IN-MLA84 ND ND ND ND <0.01 ND <0.01 <0.01

IN-MYX98 <0.01 <0.01 <0.01 <0.01 0.022 0.020 0.066 0.051

IN-N7B69 0.019 0.020 0.047 0.057 0.12 0.13 0.22 0.18

ND = Not detected (<0.002 mg/kg)
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Table 20. Residues of cyantraniliprole and metabolites in tissues from lactating cows dosed with 

cyantraniliprole for 28 days

Matrix Analyte Mean (Max) residues of cyantraniliprole and metabolites in tissues

3 mg/kg 10 mg/kg 30 mg/kg 100 mg/kg

Muscle

Cyantraniliprole <0.01

(0.011)

0.026

(0.037)

0.071

(0.092)

0.28

(0.33)

IN-HGW87 ND ND ND <0.01

IN-J9Z38 ND <0.01 0.010

(0.018)

0.027

(0.043)

IN-JCZ38 ND ND ND ND

IN-K7H19 ND ND ND ND

IN-MLA84 ND ND <0.01 <0.01

IN-MYX98 ND <0.01 <0.01 0.01

(0.011)

IN-N7B69 ND <0.01 <0.01 <0.01

Liver

Cyantraniliprole 0.054

(0.066)

0.15

(0.16)

0.46

(0.60)

1.7

(2.1)

IN-HGW87 ND ND <0.01 0.010

(0.010)

IN-J9Z38 ND <0.01 <0.01 0.015

(0.026)

IN-JCZ38 ND ND ND <0.01

(0.011)

IN-K7H19 ND ND ND ND

IN-MLA84 0.032

(0.043)

0.075

(0.099)

0.22

(0.29)

0.41

(0.57)

IN-MYX98 ND <0.01 <0.01

(0.026)

0.025

(0.025)

IN-N7B69 <0.01

(0.01)

0.021

(0.024)

0.042

(0.046)

0.076

(0.079)

Kidney

Cyantraniliprole 0.023

(0.031)

0.084

(0.14)

0.20

(0.25)

0.73

(0.89)

IN-HGW87 ND ND <0.01 0.011

(0.012)

IN-J9Z38 ND <0.01 <0.01

(0.013)

0.024

(0.031)

IN-JCZ38 ND ND ND ND

IN-K7H19 ND ND ND ND

IN-MLA84 <0.01

(0.011)

0.013

(0.017)

0.041

(0.044)

0.099

(0.13)

IN-MYX98 <0.01 <0.01 0.034

(0.039)

0.14

(0.15)

IN-N7B69 0.012

(0.015)

0.031

(0.031)

0.071

(0.081)

0.12

(0.15)

Fat

Cyantraniliprole 0.014

(0.015)

0.042

(0.066)

0.12

(0.15)

0.51

(0.58)

IN-HGW87 ND ND ND ND

IN-J9Z38 0.01

(0.012)

0.023

(0.031)

0.082

(0.12)

0.38

(0.45)

IN-JCZ38 ND ND ND ND

IN-K7H19 ND ND ND ND

IN-MLA84 ND <0.01 <0.01 <0.01

IN-MYX98 ND <0.01 <0.01 <0.01
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Table 20. Residues of cyantraniliprole and metabolites in tissues from lactating cows dosed with 

cyantraniliprole for 28 days

Matrix Analyte Mean (Max) residues of cyantraniliprole and metabolites in tissues

3 mg/kg 10 mg/kg 30 mg/kg 100 mg/kg

IN-N7B69 <0.01 <0.01 0.010

(0.012)

0.020

(0.024)

ND = Not detected (<0.002 mg/kg)

The data indicate that quantifiable residues of cyantraniliprole occur in all cattle matrices (except 

in muscle at the lowest 3 ppm dose level) at all dosing levels. Transfer factors for 

cyantraniliprole and metabolites were calculated for each matrix at each feeding level, using the 

maximum observed residue level for tissues. The calculated transfer factors are presented in 

Table 21. 

Table 21. Maximum (Mean) Residues of Cyantraniliprole in Cattle Commodities by Feeding Level.

Cattle Matrix 3.0 ppm 10 ppm 30 ppm 100 ppm

Cyantraniliprole (MW=473.7)

Milk 0.03 0.11 0.25 0.71

Muscle 0.011

(<0.01)

0.037

(0.026)

0.092

(0.071)

0.33

(0.28)

Liver 0.066

(0.054)

0.16

(0.15)

0.60

(0.46)

2.1

(1.7)

Kidney 0.031

(0.023)

0.14

(0.084)

0.25

(0.20)

0.89

(0.73)

Fat10.015

(0.014)

0.066

(0.042)

0.15

(0.12)

0.58

(0.51)

IN-J9Z38 (MW=491.7)

Milk <0.01 <0.01 0.010 0.034

Muscle <0.002 <0.01 0.018

(0.010)

0.043

(0.027)

Liver <0.002 <0.01 <0.01 0.026

(0.015)

Kidney <0.002 <0.01 0.013

(<0.01)

0.031

(0.024)

Fat10.012

(0.01)

0.031

(0.023)

0.12

(0.082)

0.45

(0.38)

IN-MLA84 (MW=441.7)

Milk <0.01 <0.01 <0.01 <0.01

Muscle <0.002 <0.002 <0.01 <0.01

Liver 0.043

(0.032)

0.099

(0.075)

0.29

(0.22)

0.57

(0.41)

Kidney 0.011

(<0.01)

0.017

(0.013)

0.044

(0.041)

0.13

(0.099)

Fat1<0.002 <0.01 <0.01 <0.01

IN-MYX98 (MW=489.7)

Milk <0.01 <0.01 0.025 0.085

Muscle <0.002 <0.01 <0.01 0.011

(0.01)
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Table 21. Maximum (Mean) Residues of Cyantraniliprole in Cattle Commodities by Feeding Level.

Cattle Matrix 3.0 ppm 10 ppm 30 ppm 100 ppm

Liver <0.002 <0.01 0.026

(<0.01)

0.025

(0.025)

Kidney <0.01 <0.01 0.039

(0.034)

0.15

(0.14)

Fat1<0.002 <0.01 <0.01 <0.01

IN-N7B69 (MW=489.7)

Milk 0.028 0.074 0.17 0.28

Muscle <0.002 <0.01 <0.01 <0.01

Liver 0.01

(<0.01)

0.024

(0.021)

0.046

(0.042)

0.079

(0.076)

Kidney 0.015

(0.012)

0.031

(0.031)

0.081

(0.071)

0.15

(0.15)

Fat1<0.01 <0.01 0.01 0.02

1

 Includes omental, perirenal, and subcutaneous fat.

Table 22. Calculated transfer factors in cattle.1,2 

Commodity 3.0 ppm 10 ppm 30 ppm 100 ppm

Cyantraniliprole

Milk 0.01 0.011 0.0083 0.0071

Muscle 0.0037 0.0037 0.0031 0.0033

Liver 0.022 0.016 0.02 0.021

Kidney 0.01 0.014 0.0083 0.0089

Fat 0.005 0.0066 0.005 0.0058

IN-J9Z38

Milk nd1nd 0.00033 0.00034

Muscle nd nd 0.0006 0.00043

Liver nd nd nd 0.00026

Kidney nd nd 0.00043 0.00031

Fat 0.004 0.0031 0.004 0.0045

IN-MLA84

Milk nd nd nd nd

Muscle nd nd nd nd

Liver 0.014 0.0099 0.01 0.0057

Kidney 0.0037 0.0017 0.0015 0.0013

Fat nd nd nd nd

IN-MYX98

Milk nd nd 0.00083 0.00085

Muscle nd nd nd 0.00011

Liver nd nd 0.00087 0.00025

Kidney nd nd 0.0013 0.0015

Fat nd nd nd nd

IN-N7B69 

Milk 0.0093 0.0074 0.0057 0.0028

Muscle nd nd nd nd
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Table 22. Calculated transfer factors in cattle.1,2 

Commodity 3.0 ppm 10 ppm 30 ppm 100 ppm

Liver 0.0033 0.0024 0.0015 0.00079

Kidney 0.005 0.0031 0.0027 0.0015

Fat nd nd 0.00033 0.0002

1

 Transfer factor calculated by dividing residue value by feeding level. nd = not determined; residues were below 

the LOQ.

2

 For all tissues, the transfer factor was calculated using the maximum residue value observed at the specified 

feeding level.

Poultry

MRID 48120015: Four treatment groups each consisting of 10 laying hens were dosed orally, 

via feed, for 28 consecutive days with cyantraniliprole at target dose rates of 3, 10 and 30 ppm

feed. Ten birds received capsules containing no cyantraniliprole as control. Additionally, 10 

birds were dosed at 30 ppm for 28 days to obtain depuration data. The dosing levels of the 

groups represent 300x, 1000x, and 3000x, respectively, the dietary burden to poultry. 

Eggs were collected from all groups daily during the dosing period. Eggs were collected twice 

daily. Samples from the afternoon collection were combined with samples from the next 

morning. Egg samples on study days -3, 1-7, 10, and 27 from the 4 treatment groups were 

analyzed. Egg yolk and egg white samples were prepared from eggs collected on days 27 and 28. 

Birds were sacrificed 4-6 hours after the final dose (except for the depuration group). 

Samples of liver (entire organ), muscle (leg and breast), and abdominal fat with skin were 

collected for analysis. All samples were stored frozen at ca -80 oC until analysis. Liver was 

extracted on the day of collection. Muscle and skin with fat were stored frozen for a maximum of 

20 days. Most samples were analyzed within 30 days and all samples were analyzed within 3 

months of collection. Storage stability data for residues of parent plus metabolites in eggs 

indicate that these compounds are stable under frozen for 97 days.

Samples of poultry (and cattle) matrices were analyzed for residues of cyantraniliprole and seven 

of its metabolites using HPLC/MS/MS (DuPont-18844). Residues were expressed in terms of 

each analyte. This method was adequate for data collection based on acceptable method 

recoveries: 80-102% for parent, 79-100% for IN-HGW87, 84-94% for IN-J9Z38, 80-94% for 

IN-JCZ38, 87-101% for IN-K7H19, 80-97% for IN-MLA84, 84-105% for IN-MYX98, and 87-

98% for IN-N7B69. The validated LOQs in each matrix were 0.01 ppm for cyantraniliprole and 

metabolites. 

Residues in whole eggs reached a plateau after ca. 3 days of dosing. Eggs seemed to contain the 

highest amount of residues, followed by liver, fat with skin and muscle. Parent residues were the 

highest in eggs, followed by IN-J9Z38, IN-MLA84, and IN-HGW87 (decreasing order). The 

results of the poultry feeding study are summarized in Table 23. 

Although cyantraniliprole was not found in liver in the poultry metabolism study, it was found as 

a major component of the residue in the feeding study, along with metabolites IN-MLA84 and 
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IN-MYX98. This may be a reflection of the longer duration of the feeding study as compared to 

the metabolism study, 28 days versus 14 days.

Table 23. Residues of cyantraniliprole and metabolites in eggs

Matrix Analyte 3 mg/kg 10 mg/kg 30 mg/kg

Mean residue (mg/kg)

Eggs

Cyantraniliprole 0.082 0.17 0.80

IN-HGW87 0.0053 0.0069 0.016

IN-J9Z38 0.039 0.077 0.41

IN-JCZ38 <0.002 <0.002 <0.002

IN-K7H19 <0.002 ND ND

IN-MLA84 0.016 0.038 0.12

IN-MYX98 0.014 0.035 0.10

IN-N7B69 <0.002 <0.002 0.0029

Table 24: Average residues of cyantraniliprole and metabolites in tissues

Matrix Analyte 3 mg/kg 10 mg/kg 30 mg/kg

Mean residue (mg/kg)

Muscle

Cyantraniliprole 0.0034 0.0093 0.025

IN-HGW87 <0.002 0.0052 0.011

IN-J9Z38 ND ND ND

IN-JCZ38 ND ND ND

IN-K7H19 ND ND ND

IN-MLA84 <0.002 <0.002 <0.002

IN-MYX98 0.0041 0.012 0.025

IN-N7B69 <0.002 <0.002 0.0026

Liver

Cyantraniliprole 0.017 0.041 0.13

IN-HGW87 0.0076 0.028 0.059

IN-J9Z38 <0.002 <0.002 <0.002

IN-JCZ38 0.0030 0.0094 0.0060

IN-K7H19 ND ND ND

IN-MLA84 0.015 0.043 0.096

IN-MYX98 0.023 0.068 0.19

IN-N7B69 0.0067 0.013 0.045

Skin with fat

Cyantraniliprole 0.0093 0.033 0.080

IN-HGW87 <0.002 0.0078 0.015

IN-J9Z38 0.0029 0.0064 0.018

IN-JCZ38 ND ND ND

IN-K7H19 ND ND ND

IN-MLA84 <0.002 0.0034 0.0080

IN-MYX98 0.0037 0.015 0.027

IN-N7B69 <0.002 <0.002 0.0030

ND – Not Detected
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The results of the poultry feeding study are summarized in Table 25. Residues of 

cyantraniliprole in eggs and tissues were below 0.1 ppm in all samples at the lowest dose group 

of 3 ppm (300X dietary burden). The parent compound was a major or the major residue.

Residue levels were dose dependent. 

Table 25. Maximum Residues of Cyantraniliprole in Hen Commodities by Feeding Level.

Hen Matrix 3.0 ppm 10 ppm 30 ppm

Cyantraniliprole (MW=473.7)

Eggs 0.082 0.17 0.80

Muscle 0.0034 0.0093 0.025

Liver 0.017 0.041 0.013

Skin with fat 0.0093 0.033 0.080

IN-J9Z38 (MW=491.7)

Eggs 0.039 0.077 0.41

Muscle ND ND ND

Liver <0.002 <0.002 <0.002

Skin with fat 0.0029 0.0064 0.018

IN-MLA84 (MW=441.7)

Eggs 0.016 0.038 0.12

Muscle <0.002 <0.002 <0.002

Liver 0.015 0.043 0.096

Skin with fat <0.002 0.0034 0.0080

IN-MYX98 (MW=489.7)

Eggs 0.014 0.035 0.10

Muscle 0.0041 0.012 0.025

Liver 0.023 0.068 0.19

Skin with fat 0.0037 0.015 0.027

IN-N7B69 (MW=489.7)

Eggs <0.002 <0.002 0.0029

Muscle <0.002 <0.002 0.0026

Liver 0.0067 0.013 0.045

Skin with fat <0.002 <0.002 0.0030

Transfer factors for cyantraniliprole and its major metabolites were calculated for each matrix at 

each feeding level, using the maximum observed residue level for eggs and tissue. The 

calculated transfer factors are presented in Table 26.

Table 26. Calculated transfer factors in poultry.1,2 

Commodity 3.0 ppm 10 ppm 30 ppm

Cyantraniliprole

Eggs 0.027 0.017 0.027

Muscle 0.0011 0.00093 0.00083

Liver 0.0057 0.0041 0.00043

Skin with fat 0.0031 0.0033 0.0027

IN-J9Z38
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Table 26. Calculated transfer factors in poultry.1,2 

Commodity 3.0 ppm 10 ppm 30 ppm

Eggs 0.013 0.0077 0.014

Muscle nd1nd nd

Liver nd nd nd

Skin with fat 0.00097 0.00064 0.0006

IN-MLA84

Eggs 0.0053 0.0038 0.004

Muscle nd nd nd

Liver 0.005 0.0043 0.0032

Skin with fat nd 0.00034 0.00027

IN-MYX98

Eggs 0.0047 0.0035 0.0033

Muscle 0.0014 0.0012 0.00083

Liver 0.0077 0.0068 0.0063

Skin with fat 0.0012 0.0015 0.0009

IN-N7B69

Eggs nd nd 0.0001

Muscle nd nd 0.000087

Liver 0.0022 0.0013 0.0015

Skin with fat nd nd 0.0001

1

 Transfer factor calculated by dividing residue value by feeding level. nd = not detected; residues were below the 

LOQ.

2

 For all tissues, the transfer factor was calculated using the maximum residue value observed at the specified 

feeding level.

Conclusions. The submitted studies are adequate to satisfy data requirements. The data indicate 

that tolerances for cyantraniliprole residues of concern are needed for milk, and fat, meat, and 

meat byproducts of cattle, goat, horse, and sheep egg, but not for eggs, poultry, and hogs. 

The maximum anticipated residues of cyantraniliprole were calculated using the estimated 

dietary burdens for cyantraniliprole (Table 18) and the maximum transfer factors for 

cyantraniliprole (Table 22 and Table 26) and are reported in Table 27 (cattle, poultry, and swine 

commodities).

Table 27. Maximum Anticipated Levels of Cyantraniliprole in Livestock Commodities Following Dosing at 1x 

the Dietary Burden.

Livestock Estimated Dietary 

Burden

(ppm)

Matrix Cyantraniliprole (ppm) Recommended Tolerance 

Level (ppm)

Cattle 0.42

1 Milk 0.0046 0.01

Muscle 0.0016 0.01

Liver 0.0092 0.01

Kidney 0.0059 0.01

Fat 0.0028 0.01

Poultry 0.011 Egg 0.00027 None

Muscle <0.00010 None

Liver <0.00010 None

Skin with fat <0.00010 None
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Swine 0.0111 Muscle <0.00010 None

Liver 0.00024 None

Kidney 0.00015 None

Fat <0.00010 None

1 Maximum estimated dietary burdens for cattle, poultry, or swine used to calculate maximum anticipated residues 

in ruminants, poultry, or hogs.

Based on the maximum anticipated residues, HED concludes that a tolerance of 0.01 ppm is 

appropriate for the sole residues of cyantraniliprole in milk, and fat, meat, and meat byproducts 

of cattle, goat, horse, and sheep. However, the residue transfer in eggs, poultry and hogs is 

negligible such that tolerances are not needed in these commodities (a §180.6(a) (3) situation). 

The residue definition for human dietary risk assessment includes cyantraniliprole and the four 

metabolites listed in Tables 21 and 26. The following levels are estimated in cattle commodities 

based on the transfer factor at 3.0 ppm: IN-J9Z38 in fat (0.0017 ppm); IN-MLA84 in liver 

(0.0059 ppm) and in kidney (0.0016 ppm); and IN-N7B69 in milk (0.0039 ppm), in liver (0.0014 

ppm), and in kidney (0.0021 ppm). While these will significantly increase the total exposure 

relative to cyantraniliprole only, the total exposure remains <0.01 ppm (LOQ) for each 

commodity, except liver (≈0.017 ppm)

The following levels are estimated in poultry commodities based on the transfer coefficient at 3.0 

ppm: IN-J9Z38 in eggs (0.00013 ppm) and in skin with fat (0.0000097 ppm); IN-MYX98 in 

eggs (0.000047 ppm), in muscle (0.000014 ppm), in liver (0.000077 ppm), and in skin with fat 

(0.000012 ppm); IN-MLA84 in eggs (0.000053 ppm) and in liver (0.00005 ppm), and IN-N7B69 

in liver (0.000022 ppm). Values for each metabolites in all poultry commodities are <0.00010 

except IN-J9Z38 in eggs at 0.00013 ppm, or about 50% of the cyantraniliprole concentration in 

eggs.

860.1500 Crop Field Trials

Monograph for Cyantraniliprole, Sections B.7.1.4 – B.7.1.12 NAFTA Field Trials; B.7.6.1.5 (MRIDs 4812007-

48120011, 48120016-48120023, 48120034, 48120037-4120038)

The registrant submitted crop field trial data from field trials conducted in North America with a 

variety of crops and greenhouse data from the EU for peppers. The relevant studies are 

described below, and the results are summarized in Table 28 through Table 126.

For the North American field trials, the petitioner collected residue data for cyantraniliprole and 

several metabolites. 

The Organization for Economic Cooperation and Development (OECD) tolerance calculation 

procedure was utilized for determining appropriate tolerance levels based on the submitted crop 

field trial data for raw agricultural crop commodities. All tolerance calculations are presented in 

Appendix II. 

An adequate number of trials with appropriate geographical distribution were submitted for each 

crop under consideration (Appendix III). Storage stability data were adequate to cover the 

intervals of all crop field trial samples. Acceptable analytical methods were used to analyze the 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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crop commodities, with acceptable concurrent recoveries demonstrated at appropriate 

fortification levels.

Various crops

Cyantraniliprole is the major component of the residues in raw agricultural crop commodities, 

although multiple analytes were determined for the field trial commodities. Generally 

metabolites contributed very litte (0 – 3%) of the cyantraniliprole concentration in the 

commodities analyzed. Formulation bridging trials on a representative group of crops have 

shown comparable residues among formulation types and methods of application, with limited

exception. In comparative residue testing of varied application methods, generally, foliar 

applications have shown higher residues than soil applications and residues for crops grown 

under protection (greenhouse) have shown higher residues than the same crops grown in the 

open field. An exception is seed treatment of potato pieces, where seed treatment plus foliar 

treatment (total application rate 0.4 lb ai/acre) yielded higher residues than foliar only treatment 

(total application rate 0.4 lb ai/acre).

Per trial conditions and results for each crop are detailed in the Mongraph. The per trial results 

may also be seen in the tables associated with the OECD statistical calculation of the tolerance 

(Appendix II). Summary results are presented here. Tables showing the number and 

geographic distribution of trials are given in Appendix III.

Tuberous and Corm Vegetables (Crop Sub-Group 1C)

Twenty-one potato field trials were conducted in the US and Canada, 3 of which were for 

processing studies and 1 of which was a decline trial. All trial sites included a treatment plot that 

received a seed treatment of Cyantraniliprole 625 g/L FS and one late season foliar application of 

Cyantraniliprole 100 g/L OD. Nineteen tests included a treatment plot that received three late 

season foliar applications of Cyantraniliprole 100 g/L OD. Four tests included a treatment plot 

that received three late season foliar applications of Cyantraniliprole 100 g/L SE. One trial 

employed in-furrow application of Cyantraniliprole 200 g/L SC at planting, followed by one late 

season foliar application of Cyantraniliprole 100 g/L OD. The crop growth stage at the time of 

foliar applications at the trial sites ranged from BBCH 43-97. For foliar applications, retreatment 

intervals were 4 – 6 days.

Sites with seed treated at the rate of 13.5 g ai/100 kg of seed with 2200 kg seed/ ha planting 

density (approximately 0.268 lb ai/A or 300 g ai/ha, or 1.5 x proposed label rate) also received a 

single application at approximately 0.134 lb ai/A (150 g ai/ha) for a seasonal maximum of 

approximately 0.402 lb ai/A/season (450 g ai/ha/season). Each foliar or furrow/foliar treated plot 

applied a seasonal maximum of approximately 0.402 lb ai/A/season (450 g ai/ha/season). For 

each foliar application, adjuvant was added to the spray mixture. Commodity samples were 

harvested at commercial maturity after the second or third applications. 

Samples were analyzed by DuPont Method 15736, Revision No. 1. Samples were extracted with 

acetonitrile/water (9/1, v/v) and analyzed by LC/MS-MS. The LOQ was 0.010 ppm for each 

analyte determined. Untreated control samples spiked with 0.010–0.10 mg/kg cyantraniliprole 

and metabolites were analyzed concurrently with the treated samples to verify method 

performance. Recoveries for all analyte/matrix combinations ranged from 68–132%, with most 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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recoveries between 70 and 120%. Although some of the recoveries are outside of the criteria 

range, there is sufficient data to validate the performance of the analytical method. 

Table 28. Summary Statistics for Potatoes from Seed + FoliarTreatments.1

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate: lb ai/A

(g ai/ha)

PHI

(days)

DuPont27583

Potato 

Tubers

625 g/L FS 

+ 100 g/L 

OD

Seed + 

Foliar

1 seed at 

planting + 1 

Foliar

0.332-0.414

(372-465) 6-8

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 42 42 42 42 42 42 42

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.024 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.016 0.010 0.010 0.010 0.010 0.010

Median 0.011 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.024 0.011 -- -- -- -- --

SDEV 0.025 0.002 -- -- -- -- --

1

Includes one in-furrow treatment (200 g/L SC) plus one foliar treatment (100 g/L OD, total rate 0.425 lb ai/ac.

Table 29. Summary Statistics for Potatoes from 3 Foliar Applications.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate: lb ai/A

(g ai/ha)

PHI

(days)

DuPont27583

Potato 

Tubers 100 g/L OD Foliar 3

(428-462) 6-8

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 38 38 38 38 38 38 38

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.034 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.034 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.012 -- -- -- -- -- --

SDEV 0.006 -- -- -- -- -- --

Table 30. Summary Statistics for Potatoes from Bridging Trials with 100g/L SE.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate: lb ai/A

(g ai/ha)

PHI

(days)

DuPont27583

Potato 

Tubers 100 g/L SE Foliar 3 0.399-0.406 (447-455) 6-7

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 10 10 10 10 10 10 10

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.035 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.016 -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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SDEV 0.009 -- -- -- -- -- --

aEP = End-use product

b

n= Number of samples; Min. = Minimum individual sample residue; Max. = Maximum individual sample residue; HAFT = 

Highest average residue from field trials; Median = Median of average sample residues; Mean = Mean of average sample 

residues 

SDEV. = Standard deviation of mean

The decline studies showed residues at or near the LOQ at all intervals.

Conclusions:

The Canada/US field potato field trial results were generated using an adequate data collection 

method. Adequate storage stability data are available on diverse crop type. The number and 

geographic distribution of trials are generally in accordance with OCSPP harmonized test 

guideline 860.1500 (Appendix III). A trial was not conducted in NAFTA growing zone 3 as 

requested, but a trial was conducted in zone 14, which is not requested by the US. The trials 

were conducted in accordance with the proposed DuPont GAP, except the seed treatment rates 

were in excess (1.5 X) of the proposed use. Also, only one trial was supplied in support of a soil 

at-planting treatment. Bridging studies indicated similar residue levels from foliar applications 

with the OD and SE formulations. Seed treatment plus foliar treatment yielded higher residues 

than foliar treatment only (total application rate 0.4 lb ai/acre). Overall, the trials will support a 

tolerance for the tuberous and corm subgroup 1C.

Metabolites were absent.

The potato seed treatment portion of the trials was conducted at 13.5 g ai/100 kg seed, or 0.013 

lb ai/cwt. This is consistent with the proposed label, which specifies a maximum of 0.01 lb ai/

cwt. The actual field trial rate based on amount ai per acre was 0.268 lb ai/acre, compared with a 

label maximum rate of 0.176 lb ai/acre.

Bulb Vegetables (Group 3-07)

IR-4 has conducted a dry bulb and green onion magnitude and decline of cyantraniliprole 

residues field study for DuPont Crop Protection in the 2009 and 2010 growing seasons. Thirteen 

MOR trials and two decline trials were conducted. All trials included a treatment plot that 

received three late season foliar applications of Cyantraniliprole 100 g/L OD. Commodity 

samplings were made within the window of commercial maturity for these plots, after the third 

application. One decline trial included a treatment plot that received two soil applications

(dripline) of Cyantraniliprole 200 g/L SC at 0.2 lb ai/acre/application. Commodity samplings 

were made within the window of commercial maturity for these plots, after the second 

application. Each treated plot applied a seasonal maximum of approximately 0.402 lb 

ai/A/season (450 g ai/ha/season). There were adjuvants added to the spray mixtures. 

Samples were analyzed by DuPont Method 15736, Revision No. 1. Samples were extracted with 

acetonitrile/water (9/1, v/v) and analyzed by LC/MS-MS. The LOQ was 0.010 ppm for each 

analyte determined. Untreated control samples spiked with 0.010–5.0 mg/kg cyantraniliprole 

and metabolites were analyzed concurrently with the treated samples to verify method 

performance. Recoveries for all analyte/matrix combinations ranged from 71–118%.The 

chromatograms of control samples of dry bulb and green onions are free from interferences. 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Recovery values were not corrected for control residues, since no residues were detected at 

levels greater than the LOQ of 0.01 ppm in any control sample.

Table 31. Summary of Statistics for Dry Bulb Onions for 3 Foliar Applications.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A

(g ai/ha)

PHI/DAAc

(days)

DuPont27436

Dry bulb 

Onions 100 g/L OD Foliar 3

0.40-0.42

(443-474) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 20 20 20 20 20 20 20

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.029 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.027 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.014 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.015 -- -- -- -- -- --

SDEV 0.006 -- -- -- -- -- --

Table 32. Summary of Statistics for Green Onions for 3 Foliar Applications.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A

(g ai/ha)

PHI/DAAc

(days)

DuPont27436

Green 

Onions 100 g/L OD Foliar 3

0.40-0.42

(452-465) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 10 10 10 10 10 10 10

Min 0.350 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.100 0.016 0.010 0.010 0.010 0.012 0.086

HAFT 4.100 0.016 0.010 0.010 0.010 0.012 0.085

Median 1.300 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.602 0.011 -- -- -- 0.010 0.028

SDEV 1.481 0.003 -- -- -- 0.001 0.031

Table 33. Summary of Statistics for Green Onions for Drip Line Soil Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A

(g ai/ha)

PHI/DAAc

(days)

DuPont27436

Green 

Onions 200 g/L SC Drip Line 

Soil 2

0.41

(454) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.034 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.035 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.035 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.035 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 63 of 193

SDEV -- -- -- -- -- -- --

aEP = End-use product

b

n= Number of individual samples; Min. = Minimum individual sample residue; Max. = Maximum individual sample residue; HAFT = Highest 

average residue from one field trial; Median = Median of average sample residues; Mean = Mean of average sample residues 

SDEV. = Standard deviation of mean

cDAA = Days after last application

Residue decline studies were conducted with dry onions and with green onions. All residues 

were non-detectable in the dry onion study. Samples of green onion were taken 1, 3, 7, and 13 

days after the last of 3 foliar applications of the OD formulation at a rate of 0.134 -0.136 lb 

ai/acre/application. The calculated half-life was 2.8 days.

Conclusions. 

The Canada/US field onion field trial results were generated using an adequate data collection 

method. Adequate storage stability data are available on diverse crop types. The number and 

geographic distribution of trials are generally in accordance with OCSPP harmonized test 

guideline 860.1500 (Appendix III). The 10 dry onion bulb trials exceed the requirement for 6 

trials, and all relevant regions are represented. The 5 green onion trials exceed the requirement 

for 3 trials. Although only one trial was conducted in region 10, where 2 trials are required, 

additional trials were conducted in regions (2 and 5) which are not required. The trials were 

conducted in accordance with the proposed DuPont GAP for foliar application of the OD 

formulation. Also, only one trial was supplied in support of the soil use of the SC formulation.

In this trial location, the OD(foliar) and SC formulations (soil drip line) were tested side-byside. The residues were greater (120X) with the foliar application. Overall, the trials will 

support tolerances for the bulb onion subgroup (3-07A) and the green onion subgroup (3-07B). 

Metabolites were absent, except from 3 foliar treatments of green onions, where IN-MYX98 and 

IN-N7B69 were each 1 – 2% of the cyantraniliprole concentration.

Leafy vegetables (except Brassica vegetables) (Group 4)

Leafy vegetables (head/leaf lettuce, celery, and spinach) were treated with with cyantraniliprole 

(OD, SC, SE formulations). Twelve trials were conducted for head lettuce, 12 trials for leaf 

lettuce, 11 trials for celery, and 10 trials for spinach. Treatments included three foliar 

applications with an OD formulation; one soil treatment with an SC formulation followed by two 

foliar applications with an OD formulation; three foliar applications with an SE formulation; two 

drip irrigation applications with an SC formulation followed by one foliar application with an 

OD formulation. The three foliar applications with the OD formulation were conducted on a plot 

at every trial site. In all cases the total application rate was about 0.4 lbs ai/acre. Each foliar 

application was about 0.135 lb ai/acre. Adjuvants were used for all foliar applications. 

Commodities were sampled at commercial maturity. For the three foliar application OD 

situation, samples were taken 1 day after the second application and 1 day after the third 

application.

Samples were analyzed by DuPont Method 15736, Revision No. 1. Samples were extracted with 

acetonitrile/water (9/1, v/v) and analyzed by LC/MS-MS. The LOQ was 0.01 ppm for each 

analyte. Untreated control samples spiked with 0.010–17.0 mg/kg cyantraniliprole and 

metabolites were analyzed concurrently with the treated samples to verify method performance. 

Recoveries for all analyte/matrix combinations ranged from 68–126%, with most recoveries 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 64 of 193

between 70 and 120%. The LOQ was 0.010 ppm for each analyte determined. The 

chromatograms of control samples of leaf lettuce, head lettuce, celery, and spinach were free 

from interferences (<0.01 ppm each).

Table 34. Summary Statistics for Head Lettuce (RAC) from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Head 

Lettuce 100 g/L OD Foliar 2 0.262-0.276 (294-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.013 0.010 0.010 0.010 0.010 0.010 0.010

Max 2.900 0.012 0.010 0.015 0.010 0.017 0.010

HAFT 2.500 0.010 0.010 0.010 0.010 0.015 0.010

Median 0.363 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.747 0.010 -- 0.010 -- 0.011 --

SDEV 0.956 0.001 -- 0.001 -- 0.002 --

Table 35. Summary Statistics for Head Lettuce (without wrapper leaf) from 2 Foliar Treatments.

Study No. Crop EPa Appl. 

Type

No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644

Head Lettuce 

(for 

consumption)

100 g/L 

OD Foliar 2 0.262-0.276 (294-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.210 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.210 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.087 -- -- -- -- -- --

SDEV 0.096 -- -- -- -- -- --

Table 36. Summary Statistics for Leaf Lettuce (RAC) from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Leaf Lettuce 100 g/L 

OD Foliar 2 0.262-0.276 (294-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.420 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.900 0.018 0.010 0.010 0.037 0.028 0.010

HAFT 4.350 0.016 0.010 0.010 0.035 0.027 0.010

Median 2.900 0.012 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 65 of 193

Mean 2.746 0.012 -- -- 0.016 0.011 --

SDEV 1.688 0.002 -- -- 0.010 0.003 --

Table 37. Summary Statistics for Celery (RAC) from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Celery 100 g/L OD Foliar 2 0.262-0.276 (294-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.68 0.010 0.010 0.010 0.010 0.010 0.010

Max 5.7 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 5.5 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.6 0.010 0.010 0.010 0.010 0.010 0.010

Mean 2.55 -- -- -- -- -- --

SDEV 2.14 -- -- -- -- -- --

Table 38. Summary Statistics for Celery (for consumption – no leaves) from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Celery for 

consumption

100 g/L 

OD Foliar 2 0.262-0.276 (294-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.40 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.4 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 4.0 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.73 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.71 -- -- -- -- -- --

SDEV 1.99 -- -- -- -- -- --

Table 39. Summary Statistics for Spinach (RAC) from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Spinach 100 g/L OD Foliar 2 0.262-0.276 (294-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 3.100 0.020 0.010 0.010 0.010 0.071 0.010

Max 14.000 0.066 0.010 0.010 0.010 0.330 0.010

HAFT 13.500 0.066 0.010 0.010 0.010 0.315 0.010

Median 6.050 0.043 0.010 0.010 0.010 0.240 0.010

Mean 7.225 0.043 -- -- -- 0.220 --

SDEV 4.491 0.017 -- -- -- 0.101 --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 66 of 193

Table 40. Summary Statistics for Head Lettuce (RAC) from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Head 

Lettuce 100 g/L OD Foliar 3 0.392-0.413 (440-464) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 24 24 24 24 24 24 24

Min 0.015 0.010 0.010 0.010 0.010 0.010 0.010

Max 2.900 0.021 0.010 0.010 0.010 0.023 0.010

HAFT 2.70 0.018 0.010 0.010 0.010 0.022 0.010

Median 0.695 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.955 0.011 -- -- -- 0.011 --

SDEV 0.855 0.003 -- -- -- 0.003 --

Table 41. Summary Statistics for Head Lettuce (for consumption) from 3 Foliar Treatments

Study No. Crop EPa Appl. 

Type

No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644

Head Lettuce 

(for 

consumption)

100 g/L 

OD Foliar 3 0.392-0.413 (440-464) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.600 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.600 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.200 -- -- -- -- -- --

SDEV 0.310 -- -- -- -- -- --

Table 42. Summary Statistics for Leaf Lettuce (RAC) from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Leaf 

Lettuce 100 g/L OD Foliar 3 0.392-0.413 (440-464) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 24 24 24 24 24 24 24

Min 0.920 0.010 0.010 0.010 0.010 0.010 0.010

Max 7.400 0.033 0.010 0.010 0.063 0.027 0.010

HAFT 6.80 0.032 0.010 0.010 0.062 0.024 0.010

Median 2.65 0.012 0.010 0.010 0.016 0.010 0.010

Mean 3.11 0.015 -- -- 0.020 0.013 --

SDEV 1.69 0.007 -- -- 0.016 0.005 --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 67 of 193

Table 43. Summary Statistics for Celery with Tops (RAC) from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Celery with 

Tops 100 g/L OD Foliar 3 0.392-0.413 (440-464) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 22 22 22 22 22 22 22

Min 0.240 0.010 0.010 0.010 0.010 0.010 0.010

Max 9.500 0.043 0.010 0.010 0.010 0.029 0.010

HAFT 9.100 0.043 0.010 0.010 0.010 0.026 0.010

Median 2.000 0.019 0.010 0.010 0.010 0.010 0.010

Mean 2.783 0.021 -- -- -- 0.012 --

SDEV 2.684 0.012 -- -- -- 0.005 --

Table 44. Summary Statistics for Celery (for consumption) from 3 Foliar Treatments.

Study No. Crop EPa Appl. 

Type

No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Celery (for 

consumption)

100 g/L 

OD Foliar 3 0.392-0.413 (440-464) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.34 0.010 0.010 0.010 0.010 0.010 0.010

Max 5.4 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 4.8 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.89 0.010 0.010 0.010 0.010 0.010 0.010

Mean 2.03 -- -- -- -- -- --

SDEV 2.91 -- -- -- -- -- --

Table 45. Summary Statistics for Spinach from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Spinach 100 g/L OD Foliar 30.392-0.413 

(440-464) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 14 14 14 14 14 14 14

Min 3.600 0.021 0.010 0.010 0.010 0.098 0.010

Max 13.000 0.093 0.010 0.010 0.015 0.410 0.012

HAFT 13.0 0.086 0.010 0.010 0.014 0.400 0.011

Median 4.60 0.034 0.010 0.010 0.010 0.225 0.010

Mean 6.24 0.048 -- -- 0.011 0.225 0.010

SDEV 3.34 0.026 -- -- 0.002 0.107 0.001

Table 46. Summary Statistics for Head Lettuce (RAC) from Soil + Foliar Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 68 of 193

DuPont25644Head 

Lettuce

200 g/L SC 

+ 100 g/L 

OD

1 soil + 2 

foliar 3 0.403-0.407 (453-457) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.017 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.017 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 47. Summary Statistics for Leaf Lettuce (RAC) from Soil + Foliar Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Leaf 

Lettuce

200 g/L SC 

+ 100 g/L 

OD

1 soil + 2 

foliar 3 0.403-0.407 (453-457) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 1.0 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.1 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 1.1 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.1 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.1 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 48. Summary Statistics for Celery (RAC) from Soil + Foliar Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Celery

200 g/L SC 

+ 100 g/L 

OD

1 soil + 2 

foliar 3 0.403-0.407 (453-457) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 3.000 0.015 0.010 0.010 0.010 0.010 0.010

Max 4.100 0.019 0.010 0.010 0.010 0.010 0.010

HAFT 3.500 0.017 0.010 0.010 0.010 0.010 0.010

Median 3.500 0.017 0.010 0.010 0.010 0.010 0.010

Mean 3.5 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 49. Summary Statistics for Spinach (RAC) from Soil + Foliar Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont- Spinach 200 g/L SC 1 soil + 2 3 0.403-0.407 (453-457) 1DAA3
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 69 of 193

25644 + 100 g/L 

OD

foliar

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 6.700 0.043 0.010 0.010 0.010 0.180 0.010

Max 6.800 0.049 0.010 0.010 0.010 0.190 0.010

HAFT 6.700 0.046 0.010 0.010 0.010 0.180 0.010

Median 6.700 0.046 0.010 0.010 0.010 0.180 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 50. Summary Statistics for Head Lettuce (RAC) from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Head 

Lettuce 100 g/L SE Foliar 3 446-466 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.120 0.010 0.010 0.010 0.010 0.010 0.010

Max 2.200 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 2.100 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.665 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.872 -- -- -- -- -- --

SDEV 0.786 -- -- -- -- -- --

Table 51. Summary Statistics for Leaf Lettuce (RAC) from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Leaf 

Lettuce 100 g/L SE Foliar 3 0.397-0.415 (446-466) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 2.300 0.010 0.010 0.010 0.012 0.010 0.010

Max 7.700 0.039 0.010 0.010 0.048 0.028 0.010

HAFT 6.800 0.038 0.010 0.010 0.045 0.023 0.010

Median 4.25 0.014 0.010 0.010 0.014 0.014 0.010

Mean 4.43 0.019 -- -- 0.020 0.016 --

SDEV 1.98 0.010 -- -- 0.013 0.006 --

Table 52. Summary Statistics for Head Lettuce (RAC) from Soil + Foliar Trial.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Head 

Lettuce

200 g/L SC

+ 100 g/L 

OD

Soil + 

Foliar

2 soil drip

1 foliar 0.401-0.403 (451-453) 1DAA3
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 70 of 193

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.810 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.000 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.910 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.910 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 53. Summary Statistics for Leaf Lettuce (RAC) from Soil + Foliar Trial.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644Leaf 

Lettuce

200 g/L SC

+ 100 g/L 

OD

Soil + 

Foliar

2 soil drip

1 foliar 0.401-0.403 (451-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 1.6 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.8 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 1.7 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.7 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.7 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 54. Summary Statistics for Celery (RAC) from Soil + Foliar Trial.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Celery

200 g/L SC

+ 100 g/L 

OD

Soil + 

Foliar

2 soil drip

1 foliar 0.401-0.403 (451-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.98 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.1 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 1.0 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.0 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.0 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 55. Summary Statistics for Spinach (RAC) from Soil + Foliar Trial.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25644 Spinach

200 g/L SC

+ 100 g/L 

OD

Soil + 

Foliar

2 soil drip

1 foliar 0.401-0.403 (451-453) 1DAA3

Statistic DPX- IN- IN- IN- IN- IN- IN-
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 71 of 193

HGW86 J9Z38 JCZ38 K7H19 MLA84 MYX98 N7B69

n

b

2 2 2 2 2 2 2

Min 6.7 0.024 0.010 0.010 0.010 0.078 0.010

Max 6.8 0.025 0.010 0.010 0.010 0.090 0.010

HAFT 6.8 0.025 0.010 0.010 0.010 0.084 0.010

Median 6.8 0.025 0.010 0.010 0.010 0.084 0.010

Mean 6.8 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

aEP = End-use product.

b

n = Number of individual specimens; Min. = Minimum individual specimen residue; Max. = Maximum individual 

specimen residue; HAFT = Highest average residue from one field trial; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of mean.

cDAA = Days after last application

A residue decline study was conducted on leaf lettuce treated two times (2 X 0.136 lb ai/acre) 

with a foliar application of the OD formulation. Lettuce samples were collected 0, 1, 3, and 5 

days after the second application. The half-life was 2.4 days.

Conclusions: 

The Canada/US field leafy vegetable field trial results were generated using an adequate data 

collection method. Adequate storage stability data are available on diverse crop. The number 

and geographic distribution of trials are in accordance with OCSPP harmonized test guideline 

860.1500 (Appendix III). Overall, the trials will support a tolerance for the leafy vegetables 

crop group (crop group 4, except Brassica vegetables). 

Metabolites were generally absent. IN-MYX98 was found in head lettuce, leaf lettuce, spinach, 

and celery from foliar treatments at 1 – 3% of the cyantraniliprole concentration.IN-J9Z38 was 

found in spinach, leaf lettuce, and head lettuce at 1% of the cyantraniliprole concentration. INMLA84 was found in leaf lettuce and spinach at 1% of the cyantranilirprole concentration.

Brassica (Cole) leafy vegetables (Group 5)

Twenty-five magnitude of residue (MOR), six bridging, and one decline/bridging trials were 

conducted. All 2008 trials included two treatment plots that received either two or three late 

season foliar applications of Cyantraniliprole 100 g/L OD. The 2008 bridging trials (three trials 

total) consisted of side-by-side plots that compared 1) the maximum use pattern comprised of 

late season foliar applications (Cyantraniliprole 100 g/L OD) to 2) the maximum soil application 

treatment (Cyantraniliprole 200 g/L SC) scenario at planting in combination with two late season 

foliar applications (Cyantraniliprole 100 g/L OD). All 2009 trials included one treatment plot 

that received three late season foliar applications of Cyantraniliprole 100 g/L OD. Commodity 

samplings were made within the window of commercial maturity for these plots, after the last 

(second or third) applications. The 2009 formulation bridging trials (four broccoli trials total) 

consisted of side-by-side plots that compared 1) the maximum use pattern comprised of late 

season foliar applications with Cyantraniliprole 100 g/L OD to 2) the maximum use pattern 

comprised of late season foliar applications with Cyantraniliprole 100 g/L SE. Each treated plot 

applied a seasonal maximum of approximately 0.402 lb ai/A/season (450 g ai/ha/season). There 

were adjuvants (methylated seed oil) added to the spray mixtures for all foliar applications.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 72 of 193

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole 

(CYANTRANILIPROLE) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, 

IN-J9Z38, and IN-K7H19) in/on Brassica (cole) leafy vegetables. Untreated control samples 

spiked with 0.010–25 mg/kg cyantraniliprole and metabolites were analyzed concurrently with 

the treated samples to verify method performance. Recoveries for all analyte/matrix 

combinations ranged from 67–126%, with most recoveries between 70 and 120%. Although a 

few of the recoveries are outside of the criteria range, there is sufficient data to validate the 

performance of the analytical method. The chromatograms of control samples of 

broccoli/cauliflower, cabbage, and mustard greens are free from interferences. Recovery values 

were not corrected for control residues, since no residues were detected at levels greater than the 

LOQ of 0.01 ppm in any control sample.

The maximum residues of cyantraniliprole, and IN-J9Z38 following 3 foliar applications (100 

g/L OD) in/on broccoli/cauliflower were 1.1 ppm, and 0.020 ppm, respectively. The maximum 

residues of cyantraniliprole, and IN-J9Z38 following 3 foliar applications (100 g/L SE) in/on 

broccoli/cauliflower were 1.1 ppm, and 0.017 ppm, respectively.

Table 56. Summary Statistics for Cabbage (RAC) from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Cabbage 

(RAC) 100 g/L OD Foliar 2 0.266-0.276 (299-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 0.220 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.820 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.680 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.500 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.488 -- -- -- -- -- --

SDEV 0.232 -- -- -- -- -- --

Table 57. Summary Statistics for Cabbage (without wrapper leaf; wowl) from 2 Foliar 

Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Cabbage 

(wowl) 100 g/L OD Foliar 2 0.266-0.276 (299-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.027 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.019 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.015 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.017 -- -- -- -- -- --

SDEV 0.007 -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 73 of 193

Table 58. Summary Statistics for Mustard Greens from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Mustard 

Greens 100 g/L OD Foliar 2 0.266-0.276 (299-310) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 4.200 0.010 0.010 0.010 0.025 0.014 0.010

Max 11.000 0.049 0.010 0.010 0.067 0.050 0.010

HAFT 10.000 0.048 0.010 0.010 0.063 0.047 0.010

Median 4.950 0.032 0.010 0.010 0.053 0.027 0.010

Mean 6.483 0.030 -- -- 0.047 0.030 --

SDEV 2.977 0.017 -- -- 0.018 0.015 --

Table 59. Summary Statistics for Broccoli/Cauliflower from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Broccoli/

Cauliflower

100 g/L 

OD Foliar 3 0.396-0.414 (445-465) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 20 20 20 20 20 20 20

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.100 0.020 0.010 0.010 0.010 0.010 0.010

HAFT 1.00 0.019 0.010 0.010 0.010 0.010 0.010

Median 0.53 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.47 0.011 -- -- -- -- --

SDEV 0.33 0.003 -- -- -- -- --

Table 60. Summary Statistics for Cabbage (with wrapper leaf; wwl) from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Cabbage 

(wwl) 100 g/L OD Foliar 3 0.396-0.414 (445-465) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 22 22 22 22 22 22 22

Min 0.270 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.980 0.015 0.010 0.010 0.010 0.010 0.010

HAFT 0.95 0.014 0.010 0.010 0.010 0.010 0.010

Median 0.56 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.556 0.010 -- -- -- -- --

SDEV 0.221 0.001 -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 74 of 193

Table 61. Summary Statistics for Cabbage (without wrapper leaf; wowl) from 3 Foliar 

Treatments.

Study No. Crop EPa Appl. Type No. of Appl. Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Cabbage 

(wowl)

100 g/L 

OD Foliar 3 0.396-0.414 (445-465) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.097 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.066 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.022 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.033 -- -- -- -- -- --

SDEV 0.033 -- -- -- -- -- --

Table 62. Summary Statistics for Mustard Greens from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Mustard 

Greens 100 g/L OD Foliar 3 0.396-0.414 (445-465) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 22 22 22 22 22 22 22

Min 2.200 0.010 0.010 0.010 0.022 0.010 0.010

Max 20.000 0.230 0.110 0.010 0.260 0.089 0.017

HAFT 19.0 0.220 0.110 0.010 0.230 0.085 0.016

Median 6.00 0.035 0.017 0.010 0.070 0.023 0.010

Mean 7.39 0.060 0.027 -- 0.086 0.034 0.011

SDEV 4.77 0.062 0.0283 -- 0.072 0.024 0.002

Table 63. Summary Statistics for Broccoli/Cauliflower from Soil + Foliar Treatment.

Study No. Crop EPa Appl. 

Type

No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641 Broccoli/Cauliflower

200 g/L 

SC + 

100 g/L 

OD

1 soil 

(shank) + 

2 foliar

3

0.402-0.405 (451-

454) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.470 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.490 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.480 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.480 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 75 of 193

Table 64. Summary Statistics for Cabbage (RAC) from Soil + Foliar Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Cabbage 

(RAC)

200 g/L SC 

+ 100 g/L 

OD

1 soil 

(shank) + 2 

foliar

3 0.402-0.405 (451-454) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.400 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.590 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.490 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.490 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 65. Summary Statistics for Mustard Greens from Soil + Foliar Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Mustard 

Greens

200 g/L SC 

+ 100 g/L 

OD

1 soil 

(shank) + 2 

foliar

3 0.402-0.405 (451-454) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 3.000 0.019 0.010 0.010 0.026 0.010 0.010

Max 3.300 0.021 0.010 0.010 0.034 0.010 0.010

HAFT 3.100 0.020 0.010 0.010 0.030 0.010 0.010

Median 3.100 0.020 0.010 0.010 0.030 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 66. Summary Statistics for Broccoli/Cauliflower from 3 Foliar Bridging Trials (100 g/L 

SE).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25641Broccoli/

Cauliflower 100 g/L SE Foliar 3 0.393-0.401 (442-451) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 0.430 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.100 0.017 0.010 0.010 0.010 0.010 0.010

HAFT 1.100 0.017 0.010 0.010 0.010 0.010 0.010

Median 0.700 0.012 0.010 0.010 0.010 0.010 0.010

Mean 0.743 0.013 -- -- -- -- --

SDEV 0.272 0.003 -- -- -- -- --

aEP = End-use product; 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 76 of 193

b

n = Number of individual specimens; Min. = Minimum individual specimen residue; Max. = Maximum individual 

specimen residue; HAFT = Highest average residue from one field trial; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of mean

cDAA = Days after last application

A decline study was conducted for broccoli that received two foliar treatments of the OD 

formulation (3X0.13 lb ai/acre) with sampling at 0, 1, 3, and 5 days after the second application. 

The half-life was calculated at 3.8 days.

Conclusions:

The Canada/US field leafy vegetable field trial results were generated using an adequate data 

collection method. Adequate storage stability data are available on diverse crop. The number 

and geographic distribution of trials are in accordance with OCSPP harmonized test guideline 

860.1500 (Appendix III). Metabolites IN-MLA84 and IN-MY98 were found at low levels (<1 –

2% cyantraniliprole concentration) in mustard greens from foliar treatment. Overall, the trials 

will support tolerances for the Brassica vegetables crop subgroups Brassica head and stem (5A) 

and Brassica leafy greens (5B).

Fruiting Vegetables (Group 8)

NAFTA Field Trials

Sixteen magnitude of residue (MOR), two bridging, and one decline/bridging trials were 

conducted in 2008 and 21 MOR trials were conducted in 2009. All trials included a treatment 

plot that received three late season foliar applications of Cyantraniliprole 100 g/L OD. 

Commodity samplings were made within the window of commercial maturity for these plots, 

after the second and third applications in 2008 and after the third application in 2009. The 

bridging trials consisted of side-by-side plots that compared 1) the maximum use pattern 

comprised of late season foliar applications (Cyantraniliprole 100 g/L OD) to 2) the maximum 

soil application treatment (Cyantraniliprole 200 g/L SC) scenario in combination with foliar 

applications (Cyantraniliprole 100 g/L OD). Each treated plot applied a seasonal maximum of 

approximately 0.4 lb ai/A/season (450 g ai/ha/season). There were adjuvants added to the spray 

mixtures.

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole 

(CYANTRANILIPROLE) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, 

IN-J9Z38, and IN-K7H19) in/on fruiting vegetables. Untreated control samples spiked with 

0.010–4.000 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treated 

samples to verify method performance. Recoveries for all analyte/matrix combinations ranged 

from 68–128%, with most recoveries between 70 and 120%. Although some of the recoveries 

are outside of the criteria range, there is sufficient data to validate the performance of the 

analytical method. The chromatograms of control samples of tomatoes, bell-peppers, and nonbell peppers were free from interferences. Recovery values were not corrected for control 

residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm in any 

control sample.

Table 67. Summary Statistics for Bell-Peppers from 2 Foliar Treatments (1DAA2).

Study No. Crop EPa Appl. Type No. of Total Rate PHI/DAAc
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 77 of 193

Appl. lb ai/A (g ai/ha) (days)

DuPont25643BellPeppers 100 g/L OD Foliar 2 0.264-0.275 (297-309) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 10 10 10 10 10 10 10

Min 0.031 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.200 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.200 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.130 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.125 -- -- -- -- -- --

SDEV 0.055 -- -- -- -- -- --

Table 68. Summary Statistics for Non-Bell Peppers from 2 Foliar Treatments (1DAA2).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25643NonBellPeppers 100 g/L OD Foliar 2 0.264-0.275 (297-309) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.410 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.400 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.305 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.293 -- -- -- -- -- --

SDEV 0.084 -- -- -- -- -- --

Table 69. Summary Statistics for Bell-Peppers from 3 Foliar Treatments (1DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25643BellPeppers 100 g/L OD Foliar 3 0.394-0.418 (443-470) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 22 22 22 22 22 22 22

Min 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.280 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.280 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.075 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.119 -- -- -- -- -- --

SDEV 0.086 -- -- -- -- -- --

Table 70. Summary Statistics for Non-Bell Peppers from 3 Foliar Treatments (1DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont- Non-Bell 100 g/L OD Foliar 3 0.394-0.418 (443-470) 1DAA3
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 78 of 193

25643 Peppers

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.066 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.470 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.420 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.186 -- -- -- -- -- --

SDEV 0.123 -- -- -- -- -- --

Table 71. Summary Statistics for Tomatoes from 3 Foliar Treatments (1DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25643 Tomatoes 100 g/L OD Foliar 3 0.394-0.418 (443-470) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 40 40 40 40 40 40 40

Min 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.280 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.260 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.080 0.010 0.010 0.010 0.010 0.010 0.010

SDEV 0.099 -- -- -- -- -- --

SDEV 0.053 -- -- -- -- -- --

Table 72. Summary Statistics for Bell-Peppers from Soil + Foliar Treatment (1DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25643BellPeppers

200 g/L SC 

+ 100 g/L 

OD

Drip 

Irrigation + 

Foliar

3 0.399-0.402 (448-452) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.088 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.095 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.092 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.092 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 79 of 193

Table 73. Summary Statistics for Non-Bell Peppers from Soil + Foliar Treatment (1DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25643Non-Bell 

Peppers

200 g/L SC 

+ 100 g/L 

OD

Drip 

Irrigation + 

Foliar

3 0.399-0.402 (448-452) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.210 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.150 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.175 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.175 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 74. Summary Statistics for Tomatoes from Soil + Foliar Treatment (1DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25643 Tomatoes

200 g/L SC 

+ 100 g/L 

OD

Drip

Irrigation + 

Foliar

3 0.399-0.402 (448-452) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.044 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.052 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.048 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.048 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

a EP = End-use Product

b n = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual 

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; Std Dev = Standard deviation of mean.

c DAA = Days After Application. 

A residue decline study was conducted on tomatoes given two foliar applications of an OD 

formulation at about 0.13 lb ai/acre/application. Samples were collected at 0, 1, 3, and 5 days 

after the last application. The cyantraniliprole residue varied from 0.054 to 0.041 ppm. While 

residues showed a pattern of decline with increasing PHI, the data were not sufficient to allow 

calculation of a half-life.

Conclusions: 

The Canada/US field fruiting vegetable field trial results were generated using an adequate data 

collection method. Adequate storage stability data are available on diverse crop types. The 

number and geographic distribution of trials are in accordance with OCSPP harmonized test 

guideline 860.1500 (Appendix III). Overall, the trials will support a tolerance for the fruiting 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 80 of 193

vegetables crop group 8. Metabolites were not quantifiable in any sample

Europe Greenhouse Trials

Fourteen protected trials were conducted on tomato and cherry tomato during 2009 growing 

season with cyantraniliprole applied as foliar treatment with an OD formulation at an application 

rate of 4x 120 g ai/ha (4 X 0.107 lb ai/acre).

Eight comparative trials were also conducted with cyantraniliprole applied via drip irrigation 

with an SC formulation and at an application rate of 4 x 100 g ai/ha (4 X 0.089 lb ai/acre).

Mature tomatoes were taken between 1 hour and 7 days after treatment. They were analysed for 

cyantraniliprole, IN-N7B69, IN-JCZ38, IN-MYX98, IN-K7H19, IN-MLA84 and IN-J9Z38 by 

validated method 1187A with a LOQ of 0.01 mg/kg for each analyte.

No residues above the LOQ of 0.01 mg/kg could be detected in any of the corresponding control 

samples. The maximum storage period of deep frozen was 240 days.

Table 75. Summary of residue data from EU protected trial

Region Commodity Application 

rate (g ai/ha)

Type of 

application 

and 

formulation

PHI 

(days)

Number 

of trials

Cyantraniliprole Residue 

levels (mg/kg)a

Min Max Median

Cyantraniliprole

Indoor Tomato 4 x120 Foliar, OD 1 9 0.043 0.22 0.14

4 x100 Drip, SC 1 5 <0.01 0.01 0.01

Cherry 

Tomato

4 x120 Foliar, OD 1 5 0.14 0.62 0.42

4 x100 Drip, SC 1 3 <0.01 0.008 0.01

a Maximum residue level refers to the sample with the highest residue at the target PHI or later

Fourteen protected pepper trials were conducted with cyantraniliprole applied as foliar treatment 

with an OD formulation and at an application rate of 4x 120 g ai/ha (4x 0.107 lb ai/acre). 

Fourteen comparative trials were also conducted with cyantraniliprole applied via drip irrigation 

with an SC formulation at an application rate of 4 x 100 g ai/ha (4x 0.89 lb ai/acre).

Mature peppers were taken between 1 hour and 14 days after treatment. They were analysed for 

cyantraniliprole, IN-N7B69, IN-JCZ38, IN-MYX98, IN-K7H19, IN-MLA84 and IN-J9Z38 by 

validated method 1187A with a LOQ of 0.01 mg/kg for each analyte. 

No residues above the LOQ of 0.01 mg/kg could be detected in any of the corresponding control 

samples. The maximum storage period of deep frozen was 240 days.

Table 76: Summary of residue data from EU protected trials

Region Commodity Application 

rate (g ai/ha)

Type of 

application 

and 

formulation

PHI 

(days)

Number 

of trials

Residue levels (mg/kg)1

Min Max Median

Cyantraniliprole

Indoor Pepper24 x120 Foliar, OD 1 14 0.01 1 0.135

4 x100 Drip, SC 1 14 <0.01 0.01 0.01

1Maximum residue level refers to the sample with the highest residue at the target PHI or later

2

Includes 5 trials with non-bell pepper (0.086 – 1 ppm).
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 81 of 193

Conclusion:

The European greenhouse results were generated using an adequate data collection method. 

Adequate storage stability data are available on diverse crop types. Greenhouse trials from nonNAFTA locations may be translated to the US. Overall, the trials will support a tolerance for the 

fruiting vegetables crop group 8. Metabolites were not determined in the European trials.

Cucurbit Vegetables (Group 9)

Twenty-five magnitude of residue (MOR), two bridging, and one decline/bridging trials were 

conducted. All trials included a treatment plot that received three late season foliar applications 

of Cyantraniliprole 100 g/L OD. Commodity samplings were made within the window of 

commercial maturity for these plots, after the second and third applications in 2008 and after the 

third application in 2009. The bridging trials consisted of side-by-side plots that compared 1) the 

maximum use pattern comprised of late season foliar applications (Cyantraniliprole 100 g/L OD) 

to 2) the maximum soil application treatment (Cyantraniliprole 200 g/L SC) scenario in 

combination with foliar applications (Cyantraniliprole 100 g/L OD). Each treated plot applied a 

seasonal maximum of approximately 0.4 lb ai/A/season (450 g ai/ha/season). There were 

adjuvants added to the spray mixtures.

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole 

(CYANTRANILIPROLE) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, 

IN-J9Z38, and IN-K7H19) in/on cucurbit vegetables (crop group 9). Untreated control samples 

spiked with 0.010–0.60 mg/kg cyantraniliprole and metabolites were analyzed concurrently with 

the treated samples to verify method performance. Recoveries for all analyte/matrix 

combinations ranged from 64–127%, with most recoveries between 70 and 120%. Although 

some of the recoveries are outside of the criteria range, there is sufficient data to validate the 

performance of the analytical method. The chromatograms of control samples of cucumber, 

summer squash, and cantaloupes were free from interferences. Recovery values were not 

corrected for control residues, since no residues were detected at levels greater than the LOQ of 

0.01 ppm in any control sample.

Table 77. Summary Statistics for Cucumbers from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Cucumber 100 g/L 

OD Foliar 2 0.262-0.281 (293-315) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 6 6 6 6 6 6 6

Min 0.032 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.036 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.060 -- -- -- -- -- --

SDEV 0.040 -- -- -- -- -- --

Table 78. Summary Statistics for Melon Rind from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 82 of 193

DuPont25642 Melon Rind 100 g/L OD Foliar 2 0.262-0.281 (293-315) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 10 10 10 10 10 10 10

Min 0.035 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.350 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.300 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.150 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.160 -- -- -- -- -- --

SDEV 0.097 -- -- -- -- -- --

Table 79. Summary Statistics for Melon Pulp from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Melon Pulp 100 g/L OD Foliar 2 0.262-0.281 (293-315) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 10 10 10 10 10 10 10

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 80. Summary Statistics for Whole Melon from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642Whole 

Melon 100 g/L OD Foliar 2 0.262-0.281 (293-315) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 10 10 10 10 10 10 10

Min 0.042 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.138 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.121 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.095 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.091 -- -- -- -- -- --

SDEV 0.029 -- -- -- -- -- --

Table 81. Summary Summer Squash from 2 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642Summer 

Squash 100 g/L OD Foliar 2 0.262-0.281 (293-315) 1DAA2

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 83 of 193

n 6 6 6 6 6 6 6

Min 0.074 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.140 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.095 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.099 -- -- -- -- -- --

SDEV 0.024 -- -- -- -- -- --

Table 82. Summary Statistics for Cucumbers from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Cucumber 100 g/L 

OD Foliar 3 0.383-0.412 (430-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 20 20 20 20 20 20 20

Min 0.016 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.200 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.043 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.057 -- -- -- -- -- --

SDEV 0.047 -- -- -- -- -- --

Table 83. Summary Statistics for Melon Rind from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Melon Rind 100 g/L OD Foliar 3 0.383-0.412 (430-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.023 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.540 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.485 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.175 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.184 -- -- -- -- -- --

SDEV 0.129 -- -- -- -- -- --

Table 84. Summary Statistics for Melon Pulp from 3 Foliar Treatments. 

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Melon Pulp 100 g/L OD Foliar 3 0.383-0.412 (430-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 84 of 193

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 85. Summary Statistics for Whole Melon from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642Whole 

Melon 100 g/L OD Foliar 3 0.383-0.412 (430-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.048 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.202 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.185 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.105 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.110 -- -- -- -- -- --

SDEV 0.043 -- -- -- -- -- --

Table 86. Summary Statistics for Summer Squash from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642Summer 

Squash 100 g/L OD Foliar 3 0.383-0.412 (430-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.059 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.061 -- -- -- -- -- --

SDEV 0.030 -- -- -- -- -- --

Table 87. Summary Statistics for Cucumbers from Soil + Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Cucumbers

200 g/L SC 

+ 100 g/L 

OD

2 Soil + 

1 Foliar 3 0.402-0.403 (452-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.058 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.010 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 85 of 193

HAFT 0.089 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.089 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 88. Summary Statistics for Melon Rind from Soil + Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Melon Rind

200 g/L SC 

+ 100 g/L 

OD

2 Soil + 

1 Foliar 3 0.402-0.403 (452-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.039 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.045 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.042 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.042 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 89. Summary Statistics for Melon Pulp from Soil + Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642 Melon Pulp

200 g/L SC 

+ 100 g/L 

OD

2 Soil + 

1 Foliar 3 0.402-0.403 (452-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 90. Summary Statistics for Whole Melon from Soil + Foliar Treatments.

d

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642Whole 

Melond

200 g/L SC 

+ 100 g/L 

OD

2 Soil + 

1 Foliar 3 0.402-0.403 (452-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.036 0.010 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 86 of 193

Max 0.037 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 91. Summary Statistics for Summer Squash from Soil + Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont25642Summer 

Squash

200 g/L SC 

+ 100 g/L 

OD

2 Soil + 

1 Foliar 3 0.402-0.403 (452-453) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.029 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.031 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

a EP = End-use Product

b n= Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual 

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; Std Dev = Standard deviation of mean.

c DAA = Days After Application. Samples were collected 1 day after application 2 or 3, as indicated.

d Values for Melon, Whole Fruit were calculated from residues determined in melon pulp and melon peel.

A decline study was conducted for cucumber treated with 3 foliar applications of cyantraniliprole 

OD formulation, each application about 0.13 lb ai/acre. Cucumber samples were taken 0.1. 3. 5, 

and 6 days after the third application. The half-life was calculated at 6.7 days.

Conclusions: 

The Canada/US field cucurbit vegetable field trial results were generated using an adequate data 

collection method. Adequate storage stability data are available on diverse crop types. The 

number and geographic distribution of trials are in accordance with OCSPP harmonized test 

guideline 860.1500 (Appendix III). Overall, the trials will support a tolerance for the cucurbit 

vegetables crop group 9. Metabolites were not quantifiable in any sample

Citrus fruit (Group 10)

The 26 trials for oranges, grapefruits, and lemons included 23 magnitude of residue trials and 

three decline trials (for soil-band application). All trials included a treatment plot that received 

three foliar applications of Cyantraniliprole 100 g/L SE at 7 day retreatment intervals. Low 

spray volumes (3 – 75 gpa) or dilute spray volumes (150 – 260 gpa) were used. Commodities 

were sampled 1 day after the third application. Three tests included a treatment plot that 

received a single soil band application of Cyantraniliprole 200 g/L SC. Commodities were 

sampled at 1, 7, and 14 days after the application. Each treated plot received a seasonal 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 87 of 193

maximum 0.382-0.413 lb ai/A/season (429-463 g ai/ha/season). For each foliar application, 

adjuvant was added to the spray mixture. The crop growth stage at the time of foliar applications 

at the trial sites ranged from BBCH 81-89 (beginning of fruit coloring to ripe for consumption).

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole 

(CYANTRANILIPROLE) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, 

IN-J9Z38, and IN-K7H19) in/on citrus fruits. Untreated control samples spiked with 0.010–

0.10 or 1.0 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treated 

samples to verify method performance. Although recoveries for all analyte/matrix combinations 

ranged from 55–141%, most of the concurrent recoveries (approximately 97%) fell within the 

range of acceptability specified by the protocol (70–120%). The chromatograms of control 

samples of 15s, grapefruits and lemons were free from interferences. Recovery values were not 

corrected for control residues, since no residues were detected at levels greater than the LOQ of 

0.010 ppm in any control sample.

In the following tables, whole commodity results are calculated from determinations of peel and 

pulp.

Table92. Summary Statistics for Whole Orange from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Whole 

Orange 100 g/L SE Foliar 3 0.382-0.413 (429-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 26 26 26 26 26 26 26

Min 0.086 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.401 0.011 0.010 0.010 0.010 0.010 0.010

HAFT 0.390 0.011 0.010 0.010 0.010 0.010 0.010

Median 0.200 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.209 0.010 -- -- -- -- --

SDEV 0.096 0.0003 -- -- -- -- --

Table 93. Summary Statistics for Orange Peel from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Orange 

Peel 100 g/L SE Foliar 3 0.382-0.413 (429-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 26 26 26 26 26 26 26

Min 0.140 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.910 0.014 0.010 0.010 0.010 0.010 0.010

HAFT 0.885 0.012 0.010 0.010 0.010 0.010 0.010

Median 0.420 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.463 0.010 -- -- -- -- --

SDEV 0.214 0.001 -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 88 of 193

Table 94. Summary Statistics for Orange Pulp from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Orange

Pulp 100 g/L SE Foliar 3 0.382-0.413 (429-463) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 26 26 26 26 26 26 26

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.092 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.087 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.039 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.045 -- -- -- -- -- --

SDEV 0.024 -- -- -- -- -- --

Table 95. Summary Statistics for Whole Lemon from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Whole 

Lemon 100 g/L SE Foliar 3 0.403-0.413 (452-462) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.111 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.304 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.300 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.198 -- -- -- -- -- --

SDEV 0.053 -- -- -- -- -- --

Table 96. Summary Statistics for Lemon Peel from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554 Lemon Peel 100 g/L SE Foliar 3 0.403-0.413 (452-462) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.240 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.630 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.625 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.405 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.413 -- -- -- -- -- --

SDEV 0.117 -- -- -- -- -- --

Table 97. Summary Statistics for Lemon Pulp from 3 Foliar Treatments.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 89 of 193

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Lemon 

Pulp 100 g/L SE Foliar 3 0.403-0.413 (452-462) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.022 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.110 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.067 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.064 -- -- -- -- -- --

SDEV 0.028 -- -- -- -- -- --

Table 98. Summary Statistics for Whole Grapefruit from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Whole 

Grapefruit 100 g/L SE Foliar 3 0.398-0.412 (446-461) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 14 14 14 14 14 14 14

Min 0.069 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.328 0.011 0.010 0.010 0.010 0.010 0.010

HAFT 0.314 0.011 0.010 0.010 0.010 0.010 0.010

Median 0.133 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.158 0.010 -- -- -- -- --

SDEV 0.076 0.0004 -- -- -- -- --

Table 99. Summary Statistics for Grapefruit Peel from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Grapefruit 

Peel 100 g/L SE Foliar 3 0.398-0.412 (446-461) 1DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 14 14 14 14 14 14 14

Min 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.770 0.019 0.010 0.010 0.010 0.010 0.010

HAFT 0.720 0.015 0.010 0.010 0.010 0.010 0.010

Median 0.340 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.386 0.011 -- -- -- -- --

SDEV 0.159 0.002 -- -- -- -- --

Table 100. Summary Statistics for Grapefruit Pulp from 3 Foliar Treatments.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27554Grapefruit 

Pulp 100 g/L SE Foliar 3 0.398-0.412 (446-461) 1DAA3
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 90 of 193

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 14 14 14 14 14 14 14

Min 0.012 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.055 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.049 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.029 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.029 -- -- -- -- -- --

SDEV 0.012 -- -- -- -- -- --

a EP = End-use Product

b

n = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual 

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; Std Dev = Standard deviation of mean

c DAA = Days After Application. 

Conclusions: 

The US field citrus (lemon, grapefruit, orange) field trial results were generated using an 

adequate data collection method. Adequate storage stability data are available on diverse crop 

types. The number and geographic distribution of trials are in accordance with OCSPP 

harmonized test guideline 860.1500 (Appendix III). A residue decline trial was not conducted 

for the foliar application scenario with citrus. Overall, the trials will support a tolerance for the 

citrus crop group 10. Metabolites were not quantifiable in any sample

Pome Fruit (Group 11)

Twenty-five magnitude of residue (MOR) trials and one decline trial were conducted. All trials 

included a treatment plot that received three late season airblast foliar applications of 

Cyantraniliprole 100 g/L SE. The retreatment interval was 7 days, and the PHI was 3 days. 

Either concentrated sprays of 10 – 80 GPA or dilute spray volumes of 100 – 300 GPA were 

used. Commodities were sampled within the window of commercial maturity for these plots, 

after the third application. Each treated plot applied a seasonal maximum of approximately 

0.40 lb ai/A/season (45 g ai/ha/season). For each foliar application, adjuvant was added to the 

spray mixture. The crop growth stage at the time of foliar applications at the trial sites ranged 

from BBCH 75-81 (first application), BBCH 81-85 (second application), and BBCH 87-89 (third 

application).

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole 

(CYANTRANILIPROLE) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, 

IN-J9Z38, and IN-K7H19) in/on pome fruits. Untreated control samples spiked with 0.010–

1.00 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treated samples 

to verify method performance. Although recoveries for all analyte/matrix combinations ranged 

from 61–125%, most recoveries were between 70 and 120%. The chromatograms of control 

samples of apples and pears were free from interferences. Recovery values were not corrected 

for control residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm 

in any control sample.

Note that samples were analyzed for both 3 day and 7 day PHIs. The proposed labels are for a 3 

day PHI.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 91 of 193

Table 101. Summary Statistics for Pears from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27438 Pears 100 g/L SE Foliar 3 0.398-0.411 (446-460) 3DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 20 20 20 20 20 20 20

Min 0.074 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.650 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.535 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.185 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.278 -- -- -- -- -- --

SDEV 0.197 -- -- -- -- -- --

Table 102. Summary Statistics for Pears from 3 Foliar Treatments (6-8DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27438 Pears 100 g/L SE Foliar 3 0.398-0.411 (446-460) 6-8DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 20 20 20 20 20 20 20

Min 0.034 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.590 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.560 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.239 -- -- -- -- -- --

SDEV 0.181 -- -- -- -- -- --

Table 103. Summary Statistics for Apples from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27438 Apples 100 g/L SE Foliar 3 0.398-0.411 (446-460) 3DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 32 32 32 32 32 32 32

Min 0.046 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.310 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.310 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.155 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.168 -- -- -- -- -- --

SDEV 0.079 -- -- -- -- -- --

Table 104. Summary Statistics for Apples from 3 Foliar Treatments (6-8DAA3).

Study No. Crop EPa Appl. Type No. of Total Rate PHI/DAAc
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 92 of 193

Appl. lb ai/A (g ai/ha) (days)

DuPont27438 Apples 100 g/L SE Foliar 3 0.398-0.411 (446-460) 6-8DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 32 32 32 32 32 32 32

Min 0.052 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.310 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.300 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.125 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.142 -- -- -- -- -- --

SDEV 0.073 -- -- -- -- -- --

a EP = End-use Product

b N = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual 

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; Std Dev = Standard deviation of mean

c DAA = Days After Application. Samples were collected 3 or 6 days after application, as indicated. Note that the 

proposed label has a 3 day PHI.

A residue decline study was conducted on apples. Samples were collected before the third foliar 

application and at day 0, 1, 3, and 6 after the third treatment. The calculated half-life was 17 

days.

Conclusion

The US field pome fruit (apple, pear) field trial results were generated using an adequate data 

collection method. Adequate storage stability data are available on diverse crop types. The 

number and geographic distribution of trials are in accordance with OCSPP harmonized test 

guideline 860.1500 (Appendix III). Overall consistent residue behavior was found in pome fruit 

data (crop group 11-09) from NAFTA regions for application and sampling conducted according 

to critical GAP (cGAP) and the specified use pattern, providing data appropriate for setting 

tolerances/MRLs. Metabolites were not quantifiable in any sample.

Stone Fruit (Group 12-12)

Twenty-nine tests for magnitude and/or decline of residue purposes were categorized as follows: 

28 magnitude of residue (MOR), and one decline/MOR. All tests included a treatment plot that 

received three late season airblast foliar applications of Cyantraniliprole 100 g/L SE at about 

0.13 lb ai/acre/application with either low volume (13 – 75 gpa) or high volume (100 -250 gpa). 

Commodity samplings were made within the window of commercial maturity for these plots, just 

before or after the third application. Each treated plot applied a seasonal maximum of 

approximately 0.402 lb ai/A/season (450 g ai/ha/season). For each foliar application, adjuvant 

was added to the spray mixture. The crop growth stage at the time of foliar applications at the 

trial sites ranged from BBCH 75-89. Note that the proposed label PHI is 3 days.

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole 

(CYANTRANILIPROLE) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, 

IN-J9Z38, and IN-K7H19) in/on stone fruits. Untreated control samples spiked with 0.010–

2.000 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treated 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 93 of 193

samples to verify method performance. Recoveries for all analyte/matrix combinations ranged 

from 73-131%, with most recoveries between 70 and 120%. Although some of the recoveries 

were outside of the criteria range, there is sufficient data to validate the performance of the 

analytical method. The chromatograms of control samples of peaches, plums, and cherries are 

free from interferences. Recovery values were not corrected for control residues, since no 

residues were detected at levels greater than the LOQ of 0.01 ppm in any control sample.

Table 105. Summary Statistics for Peaches from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27437 Peaches 100 g/L SE Foliar 3 0.398-0.413 (446-463) 3DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 26 26 26 26 26 26 26

Min 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.400 0.025 0.010 0.010 0.010 0.010 0.010

HAFT 0.960 0.024 0.010 0.010 0.010 0.010 0.010

Median 0.340 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.386 0.012 -- -- -- -- --

SDEV 0.224 0.004 -- -- -- -- --

Table 106. Summary Statistics for Peaches from 3 Foliar Treatments (7DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27437 Peaches 100 g/L SE Foliar 3 0.398-0.413 (446-463) 7DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 26 26 26 26 26 26 26

Min 0.100 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.930 0.037 0.010 0.010 0.010 0.010 0.010

HAFT 0.890 0.032 0.010 0.010 0.010 0.010 0.010

Median 0.230 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.313 0.013 -- -- -- -- --

SDEV 0.216 0.007 -- -- -- -- --

Table 107. Summary Statistics for Plums from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27437 Plums 100 g/L SE Foliar 3 0.397-0.413 (445-463) 3DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.028 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.300 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.280 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.066 0.010 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Mean 0.104 -- -- -- -- -- --

SDEV 0.084 -- -- -- -- -- --

Table 108. Summary Statistics for Plums from 3 Foliar Treatments (7DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27437 Plums 100 g/L SE Foliar 3 0.397-0.413 (445-463) 7DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.028 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.300 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.290 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.060 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.097 -- -- -- -- -- --

SDEV 0.082 -- -- -- -- -- --

Table 109. Summary Statistics for Cherries from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27437 Cherries 100 g/L SE Foliar 3 0.388-0.415 (434-465) 3DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 14 14 14 14 14 14 14

Min 0.290 0.010 0.010 0.010 0.010 0.010 0.010

Max 3.900 0.053 0.010 0.010 0.010 0.011 0.011

HAFT 3.800 0.052 0.010 0.010 0.010 0.011 0.011

Median 0.930 0.027 0.010 0.010 0.010 0.010 0.010

Mean 1.179 0.026 -- -- -- 0.010 0.010

SDEV 1.190 0.015 -- -- -- -- --

Table 110. Summary Statistics for Cherries from 3 Foliar Treatments (7DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27437 Cherries 100 g/L SE Foliar 3 0.388-0.415 (434-465) 7DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 14 14 14 14 14 14 14

Min 0.190 0.010 0.010 0.010 0.010 0.010 0.010

Max 3.100 0.061 0.010 0.010 0.010 0.011 0.010

HAFT 2.200 0.052 0.010 0.010 0.010 0.011 0.010

Median 0.790 0.027 0.010 0.010 0.010 0.010 0.010

Mean 0.880 0.027 -- -- -- 0.010 --

SDEV 0.719 0.017 -- -- -- -- --

aEP = End-use product
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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b

n = Number of individual specimens; Min. = Minimum individual specimen residue 

Max. = Maximum individual specimen residue; HAFT = Highest average residue from one field trial; Median = 

Median of average specimen residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of 

mean. c DAA = Days after application or PHI. Note that the proposed label PHI is 3 days.

A residue decline study was conducted with peaches. Samples were collected before the third 

application and on days 0, 1, 3, and 7 after the third foliar application. The calculated half-life is 

12 days.

Conclusion:

The US field stone fruit (peach, plum, cherry) field trial results were generated using an adequate 

data collection method. Adequate storage stability data are available on diverse crop types. The 

number and geographic distribution of trials are in accordance with OCSPP harmonized test 

guideline 860.1500 (Appendix III). Overall consistent residue behavior was found in stone fruits 

(crop group 12-09) data from NAFTA regions for application and sampling conducted according 

to critical GAP (cGAP), providing data appropriate for setting tolerances/MRLs Metabolite INMYX98 was found at a maximum concentration of 0.011 ppm in cherries. 

Small fruit and berries (Group 13-07)

Eight magnitude of residue (MOR) trials and two decline trials were conducted on blueberries. 

All trials included a treatment plot that received three foliar applications of Cyantraniliprole 100 

g/L SE. Two trials received a seasonal maximum of approximately 0.134 lb ai/A/season (150 g 

ai/ha/season). The remaining trials received a seasonal maximum of approximately 0.402 lb 

ai/A/season (450 g ai/ha/season). The PHI was 3 days. There were adjuvants added to the spray 

mixtures. The crop growth stage at the time of foliar applications at the 0.134 lb ai/A/season 

trial sites ranged from BBCH 79-88. At the remaining sites, the crop growth stage at the time of 

the first foliar application ranged from 4% to 80% mature berries; and from 60% to 100% ripe 

berries at the last application.

DuPont Method 15736, LC-MS/MS, was suitable to quantitate residues of cyantraniliprole and 

the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, IN-J9Z38, and IN-K7H19) 

in/on blueberries. Untreated control samples spiked with 0.010–0.100 or 2.0 mg/kg 

cyantraniliprole (CYANTRANILIPROLE) and metabolites were analyzed concurrently with the 

treated samples to verify method performance. Concurrent recoveries for residues of 

cyantraniliprole generally fell within the range of acceptability specified by the protocol (70 to 

120%). Recoveries for all analytes ranged from 76 to 127%. The chromatograms of control 

samples of blueberries are free from interferences. Recovery values were not corrected for 

control residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm in 

any control sample.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Table 111. Summary Statistics for Blueberries from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A(g ai/ha)

PHI/DAAc

(days)

DuPont27971 Blueberries 100 g/L SE Foliar 3 0.134 (150) 3DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 4 4 4 4 4 4 4

Min 0.430 0.010 0.01 0.01 0.01 0.010 0.01

Max 1.500 0.014 0.01 0.01 0.01 0.011 0.01

HAFT 1.500 0.013 0.01 0.01 0.01 0.011 0.01

Median 1.055 0.011 0.01 0.01 0.01 0.010 0.01

Mean 1.010 0.012 -- -- -- 0.010 --

SDEV 0.571 0.002 -- -- -- 0.001 --

Table 112. Summary Statistics for Blueberries from 3 Foliar Treatments (2-4DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A

(g ai/ha)

PHI/DAAc

(days)

AAFC09-

025R Blueberries 100 g/L SE Foliar 3

0.397–0.408

(445-458) 2-4DAA3

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 16 16 16 16 16 16 16

Min 0.370 0.010 0.01 0.01 0.01 0.010 0.01

Max 2.000 0.019 0.01 0.01 0.01 0.015 0.01

HAFT 1.950 0.017 0.01 0.01 0.01 0.015 0.01

Median 0.650 0.010 0.01 0.01 0.01 0.010 0.01

Mean 0.880 0.012 -- -- -- 0.011 --

SDEV 0.536 0.003 -- -- -- 0.002 --

aEP = End-use product

b

n = Number of individual specimens ; Min. = Minimum individual specimen residue ; Max. = Maximum individual 

specimen residue; HAFT = Highest average residue from one field trial ; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues ; SDEV. = Standard deviation of mean

c DAA = Days after application

Two decline trials (one each in Region 1 and Region 5) were conducted for blueberries receiving 

3 applications at a total rate of 0.40 lb ai/acre/season. Samples were taken at 0, 2, 7, and 8 days 

after the final treatment and at 0.4, 7, and 10 days after the final treatment. The calculated halflives were 4.2 and 3.8 days.

Conclusion:

The NAFTA field blueberry field trial results were generated using an adequate data collection 

method. Adequate storage stability data are available on diverse crop types. The number and 

geographic distribution of trials are in accordance with OCSPP harmonized test guideline 

860.1500 (Appendix III). Overall consistent residue behavior was found in blueberry data (crop 

subgroup 13-07B) from NAFTA regions for application and sampling conducted according to 

critical GAP (cGAP), providing data appropriate for setting tolerances/MRLs. Metabolite INMYX98 was found at a maximum concentration of 0.015 ppm in blueberries. 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Tree Nuts (Crop Group 14)

Twelve magnitude of residue (MOR) trials were conducted. All trials included a treatment plot 

that received three foliar/airblast applications of Cyantraniliprole 100 g/L OD (TRT 1). Two 

trials included a treatment plot that received three foliar applications of Cyantraniliprole 100 g/L 

SE (TRT 2). One trial included a treatment plot that received one shank or subsurface side-dress 

application of Cyantraniliprole 200 g/L SC (TRT 3). Commodity samplings were made 5DAA3 

(TRTs 1 and 2) or within the window of commercial maturity (TRT 3). Each treated plot 

applied a seasonal maximum of approximately 0.402 lb ai/A/season (450 g ai/ha/season). There 

were adjuvants added to the spray mixtures. The crop growth stage at the time of foliar 

applications at the trial sites ranged from BBCH 75-97, or hulls splitting to shucks split and dry.

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole and the 

metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, IN-J9Z38, and IN-K7H19) in/on 

tree nuts. Untreated control samples spiked with 0.010-6.0 mg/kg cyantraniliprole and 

metabolites were analyzed concurrently with the treated samples to verify method performance. 

Most of the concurrent recoveries fell within the range of acceptability specified by the protocol 

(70–120%). Recoveries for all analyte/matrix combinations ranged from 69-120%. Therefore,

the analytical methods used perform well for the determination of cyantraniliprole and 

metabolites in treated crops. The chromatograms of control samples of almond and pecan are 

free from interferences. Recovery values were not corrected for control residues, since no 

residues were detected at levels greater than the LOQ of 0.01 ppm in any control sample.

Table 113. Summary Statistics for Almond Nutmeat from 3 Foliar Treatments (100 g/L OD).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha) PHI (days)

DuPont27446Almond 

Nutmeat 100 g/L OD Foliar 3 0.390-0.410 (437-459) 5

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.024 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.023 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.013 -- -- -- -- -- --

SDEV 0.005 -- -- -- -- -- --

Table 114. Summary Statistics for Almond Nutmeat from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27446Almond 

Nutmeat 100 g/L SE Foliar 3 0.404-0.416 (453-458) 5

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 4 4 4 4 4 4 4

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.019 0.010 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 98 of 193

HAFT 0.018 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.013 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.014 -- -- -- -- -- --

SDEV 0.004 -- -- -- -- -- --

Table 115. Summary Statistics for Almond Hulls from 3 Foliar Treatments (100 g/L OD).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha) PHI (days)

DuPont27446Almond 

Hulls 100 g/L OD Foliar 3 0.390-0.410 (437-459) 5

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.780 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.600 0.026 0.010 0.010 0.010 0.015 0.010

HAFT 4.60 0.025 0.010 0.010 0.010 0.014 0.010

Median 1.60 0.013 0.010 0.010 0.010 0.010 0.010

Mean 2.082 0.014 -- -- -- 0.011 --

SDEV 1.447 0.006 -- -- -- 0.002 --

Table 116. Summary Statistics for Almond Hulls from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27446Almond 

Hulls 100 g/L SE Foliar 3 0.390-0.410 (437-459) 5

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 4 4 4 4 4 4 4

Min 0.780 0.012 0.010 0.010 0.010 0.010 0.010

Max 4.600 0.041 0.010 0.010 0.010 0.010 0.010

HAFT 4.550 0.038 0.010 0.010 0.010 0.010 0.010

Median 1.550 0.026 0.010 0.010 0.010 0.010 0.010

Mean 2.070 0.026 -- -- -- -- --

SDEV 1.370 0.014 -- -- -- -- --

Table 117. Summary Statistics for Pecan Nutmeat from 3 Foliar Treatments (100 g/L OD).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI 

(days)

DuPont27446Pecan 

Nutmeat 100 g/L OD Foliar 3 0.40–0.42 (445-465) 4-5

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 12 12 12 12 12 12 12

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 99 of 193

SDEV -- -- -- -- -- -- --

Table 118. Summary Statistics for Pecan Nutmeat from Soil Treatment.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27446Pecan 

Nutmeat 200 g/L SC Soil 1 0.41 (462) 57

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

aEP = End-use product

b

n = Number of individual specimens ; Min. = Minimum individual specimen residue 

Max. = Maximum individual specimen residue ; HAFT = Highest average residue from one field trial;Median = 

Median of average specimen residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of 

mean

Conclusion:

The NAFTA tree nut field trial results were generated using an adequate data collection method.

Adequate storage stability data are available on diverse crop types. The number and geographic 

distribution of trials are in accordance with OCSPP harmonized test guideline 860.1500. Overall 

consistent residue behavior was found in almond and pecan data (crop group 14-11; Tree Nuts) 

from NAFTA regions for application and sampling conducted according to critical GAP (cGAP), 

providing data appropriate for setting tolerances/MRLs. Metabolite IN-MYX98 was found at a 

maximum concentration of 0.015 ppm in almond hulls with the OD formulation.

Oilseed (Group 20)

Cottonseed

The 13 cotton trials included sampling for magnitude and/or decline of residue purposes: 10 

magnitude of residue (MOR), one bridging, and two decline. All included a treatment plot that 

received three late season foliar applications of Cyantraniliprole 100 g/L OD. Commodity 

samplings were made within the window of commercial maturity for these plots, after the second 

and third applications. The bridging trial consisted of side-by-side plots that compared 1) the 

maximum use pattern comprised of late season foliar applications (Cyantraniliprole 100 g/L OD) 

to 2) the maximum soil application treatment (Cyantraniliprole 200 g/L SC) scenario in 

combination with foliar applications (Cyantraniliprole 100 g/L OD). Each treated plot received a 

seasonal maximum of approximately 0.402 lb ai/A/season (450 g ai/ha/season). For each foliar 

application, adjuvant was added to the spray mixture. The crop growth stage at the time of foliar 

applications at the trial sites ranged from BBCH 78-100, and BBCH 00 (at planting) for the soil 

application.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 100 of 193

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole (DPXHGW36) and the metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, IN-J9Z38, and 

IN-K7H19) in/on cotton. Untreated control samples spiked with 0.010–1.6 mg/kg 

cyantraniliprole and metabolites were analyzed concurrently with the treated samples to verify 

method performance. Most of the concurrent recoveries fell within the range of acceptability 

specified by the protocol (70–120%). Recoveries for all analyte/matrix combinations ranged 

from 59–128%. Although some of the recoveries were outside of the criteria range, there is 

sufficient data to validate the performance of the analytical method. The chromatograms of 

control samples of cotton are free from interferences. Recovery values were not corrected for 

control residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm in 

any control sample.

Table 119. Summary Statistic for Cottonseed from Treatment # 3 (bridging trial).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27565 Cottonseed

100 g/L 

OD

200 g/L SC

In-Furrow 

Soil + 

Foliar

1 + 2 0.398 (446) 8

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.14 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.16 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.15 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.15 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.15 -- -- -- -- -- --

SDEV -- -- -- -- -- -- --

Table 120. Summary Statistic for Cottonseed from Treatment # 3 (bridging trial).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27565 Cottonseed 100 g/L 

OD Foliar 3 0.398 (446) 8

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 2 2 2 2 2 2 2

Min 0.27 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.32 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.295 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.295 0.010 0.010 0.010 0.010 0.010 0.010

Mean -- -- -- -- -- -- --

SDEV -- -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 101 of 193

Table 121. Summary Statistic for Cottonseed from Treatment # 1.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27565 Cottonseed 100 g/L 

OD Foliar 3 0.398-0.416 (446-466) 6-9

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 26 26 26 26 26 26 26

Min 0.011 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.200 0.064 0.010 0.010 0.010 0.010 0.010

HAFT 0.990 0.061 0.010 0.010 0.010 0.010 0.010

Median 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.212 0.015 -- -- -- -- --

SDEV 0.257 0.014 -- -- -- -- --

Table 122. Summary Statistic for Cotton Gin Byproduct from Treatment # 1.

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI/DAAc

(days)

DuPont27565

Cotton

Gin 

Byproduct

100 g/L 

OD Foliar 3 0.398-0.416 (446-466) 6-9

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 2.600 0.015 0.010 0.010 0.010 0.010 0.010

Max 5.700 0.077 0.010 0.010 0.010 0.027 0.010

HAFT 5.000 0.0695 0.010 0.010 0.010 0.025 0.010

Median 3.150 0.0495 0.010 0.010 0.010 0.0165 0.010

Mean 3.475 0.0469 -- -- -- 0.0173 --

SDEV 1.074 0.0268 -- -- -- 0.006 --

aEP = End-use product

b

n= Number of individual specimens ; Min. = Minimum individual specimen residue 

Max. = Maximum individual specimen residue; HAFT = Highest average residue from one field trial; Median = 

Median of average specimen residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of 

mean

cDAA = Days after last application

Residue decline studies were conducted in region 4 and regions 6. Cottonseed was sampled at 0, 

1, 5, and 7 days after the third foliar application. The half-lives were calculated as 3.7 and 5.2 

days.

Conclusion: 

The NAFTA cottonseed field trial results were generated using an adequate data collection 

method. Adequate storage stability data are available on diverse crops. The number and 

geographic distribution of trials are in accordance with OCSPP harmonized test guideline 

860.1500 (Appendix III). Overall consistent residue behavior was found in cotton data from 

NAFTA regions for application and sampling conducted according to critical GAP (cGAP), 

providing data appropriate for setting tolerances/MRLs. Metabolite IN-MYX98 was found at a 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 102 of 193

maximum concentration of 0.027 ppm in cotton gin byproducts. This is <1% of the 

cyantraniliprole concentration.

Canola and Sunflower

The 26 trials (17 canola and 9 sunflower) that included sampling for magnitude of residue 

purposes were categorized as follows: 26 magnitude of residue (MOR) including 5 MOR seed 

treatment trials. All trials included a treatment plot that received three late season foliar 

applications of Cyantraniliprole 100 g/L OD. For the seed treatment trials a second plot was 

planted to seed treated with Cyantraniliprole 625 g/L FS, then received three late-season foliar 

applications of Cyantraniliprole 100 g/L OD. Commodity samplings were made within the 

window of commercial maturity for these plots, after the third and fourth applications. Each 

treated plot applied a seasonal maximum of approximately 0.402 lb ai/A/season (450 g 

ai/ha/season). For each foliar application, adjuvant was added to the spray mixture. The crop 

growth stage at the time of foliar applications at the trial sites ranged from BBCH 72-93.

DuPont Method 15736, LC-MS/MS, was used to quantitate residues of cyantraniliprole and the 

metabolites (IN-N7B69, IN-JCZ38, IN-MYX98, IN-MLA84, IN-J9Z38, and IN-K7H19) in/on 

oilseed. Untreated control samples spiked with 0.010–1.0 mg/kg cyantraniliprole and metabolites 

were analyzed concurrently with the treated samples to verify method performance. Recoveries 

for all analyte/matrix combinations ranged from 62–131%, with the majority of the concurrent 

recoveries within the range of acceptability specified by the protocol (70–120%). Although some 

of the recoveries were outside of the criteria range, there is sufficient data to validate the 

performance of the analytical method. The chromatograms of control samples of canola and 

sunflower are free from interferences. Recovery values were not corrected for control residues, 

since no residues were detected at levels greater than the LOQ of 0.01 ppm in any control 

sample.

Side-by-side trials to compare 3 foliar applications in/on canola versus a seed treatment followed 

by 3 foliar applications indicated that maximum residues of cyantraniliprole were 0.132 ppm, 

and 0.098 ppm, respectively, when harvested 7 days after the last application.

Data from one of the trial sites (Trial 3, St. Marc-sur-Richelieu, QC, Canada, 2009), were not 

considered in the statistics (Table 123) since samples of canola seed were harvested 1 day after 

the last application instead of 7 days.

Table 123. Summary Statistics for Canola Seeds From Treatment # 1 (Bridging Trial; 7DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27582Canola 

Seeds 100 g/L OD Foliar 3 Foliar 0.400-0.406 (448-455) 6-7

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.340 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.315 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.039 0.010 0.010 0.010 0.010 0.010 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Mean 0.104 -- -- -- -- -- --

SDEV 0.132 -- -- -- -- -- --

Table 124. Summary Statistics for Canola Seeds From Treatment # 2 (Bridging Trial; 7DAA4).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27582Canola 

Seeds

625 g/L FS 

+ 100 g/L 

OD

Seed + 

Foliar

1 Seed + 3 

Foliar

0.070 + 0.333-0.335

(78.6 + 373-375) 6-7

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 8 8 8 8 8 8 8

Min 0.015 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.220 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.200 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.040 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.077 -- -- -- -- -- --

SDEV 0.086 -- -- -- -- -- --

Table 125. Summary Statistics for Canola Seeds From Treatment # 1 (7DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27582Canola

Seeds 100 g/L OD Foliar 3 0.396-0.409 (444-458) 7

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 32 32 32 32 32 32 32

Min 0.016 0.010 10 10 10 10 10

Max 0.650 0.025 0.010 0.010 0.010 0.010 0.010

HAFT 0.610 0.022 0.010 0.010 0.010 0.010 0.010

Median 0.075 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.148 0.012 0.010 0.010 0.010 0.010 0.010

SDEV 0.157 0.003 -- -- -- -- --

Table 126. Summary Statistics for Sunflower Seeds From Treatment # 1 (7DAA3).

Study No. Crop EPa Appl. Type No. of 

Appl.

Total Rate

lb ai/A (g ai/ha)

PHI

(days)

DuPont27582Sunflower 

Seeds 100 g/L OD Foliar 3 0.394-0.407 (441-456) 5-7

Statisticb DPXHGW86

INJ9Z38INJCZ38INK7H19INMLA84INMYX98INN7B69

n 18 18 18 18 18 18 18

Min 0.026 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.360 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.320 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.069 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.100 -- -- -- -- -- --
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 104 of 193

SDEV 0.088 -- -- -- -- -- --

aEP = End-use product

bN = Number of individual specimens; Min. = Minimum individual specimen residue; Max. = Maximum individual 

specimen residue; HAFT = Highest average residue from one field trial ; Median = Median of average specimen 

residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of mean

Conclusions

The NAFTA canola and sunflower field trial results were generated using an adequate data 

collection method. Adequate storage stability data are available on diverse crops. The number 

and geographic distribution of trials are in accordance with OCSPP harmonized test guideline 

860.1500 (Appendix III). Overall consistent residue behavior was found in cotton data from 

NAFTA regions for application and sampling conducted according to critical GAP (cGAP), 

providing data appropriate for setting tolerances/MRLs. Metabolites were not quantifiable in 

any sample.

The canola seed treatment was conducted at 0.07 lb ai/acre. The label sepecifies a maximum 

treatment rate of 1 lb ai/cwt seed. Canola is planted at an average rate of 6 lbs canola seed per 

acre (OK State University; Government of Saskatchewan). This is equivalent to 0.06 lb ai/acre. 

Therefore the trials are reflective of the proposed use. Canola seed treatment use results may be 

translated to sunflower.

860.1520 Processed Food and Feed

Monograph for Cyantraniliprole, Sections B.7.1.4 through B7.1.2 (MRIDs 48120044, 48120011, 48120012, 

48120017, 48120018, 48120020, 48120021, 48120023)

Nature of the residue in processed commodities

Note: This is not a guideline requirement in NAFTA countries.

MRID 48120044: Hydrolysis of radiolabelled cyantraniliprole at 1.0 mg ai/L was studied at 90, 

100, and 120 C at pH 4, 5, and 6, respectively, using aqueous citrate buffer. The test solutions 

were placed in 12-mL glass vessels and the headspace was minimized. Acetonitrile (1%) was 

used as a co-solvent. Samples were analyzed at zero time, after 20 min. (pH 4 and 6) and 1 h (pH 

5) by LSC and HPLC. Identification of parent and transformation products was by cochromatography and the identifications made were confirmed using LC-MS analysis. The limit 

of quantification (LOQ) for both labeled forms was 1% applied radioactivity (AR). Results are 

tabulated below.

Table 127. Distribution of radioactivity for [CN14C]-cyantraniliprole from simulated processing 

conditions

Component

pH 4 pH 5 pH 6

Controld90 C Controld100 C Controld120 C

Cyantraniliprole (parent) 97.54 90.56 96.73 74.54 96.92 93.58

IN-J9Z38 0.69 4.40 1.14 11.51 0.92 6.21

IN-N5M09 ND 1.90 ND 8.41 ND ND

Othersa ND 0.70 0.32 3.39 0.47 1.64

Apparatus washb0.77 0.84 0.80 0.74 0.74 0.68

Total % recoveryc99.00 98.40 98.99 98.59 98.92 102.11

a: Sum of minor peaks (individually 5% AR).
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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b: Apparatus wash radioactivity not characterized.

c: Recovery of administered radioactivity as determined by LSC analyses of the dosing solution.

d

Indicated pH at ambient temperature.

ND = Not detected

Table 128. Distribution of radioactivity for [PC14C]-cyantraniliprole from simulated processing 

conditions

Component

pH 4 pH 5 pH 6 

Controlb90 C Controlb100 C Controlb120 C

Cyantraniliprole 97.00 89.23 94.71 75.12 98.43 91.49

IN-J9Z38 0.14 5.36 1.05 13.64 0.36 3.71

IN-F6L99 ND 1.71 ND 5.33 ND 0.12

Othersa0.80 1.70 1.33 4.04 1.26 2.14

Total % recovery 97.94 97.99 97.09 98.12 100.05 97.46

a: Sum of minor peaks (individually 5% AR).

b

Indicated pH at ambient temperature.

ND = Not detected

Cyantraniliprole was susceptible to hydrolysis under conditions representative of pasteurization 

and sterilization, resulting in the formation of IN-J9Z38. Further degradation of cyantraniliprole 

occurred under hydrolytic conditions representative of baking, brewing, or boiling at 100 C for 

60 min in pH 5 solution, resulting in the formation of IN-J9Z38, IN-F6L99, and IN-N5M09.

Degradation pathway for cyantraniliprole at high temperature hydrolysis conditions

Magnitude of the Residue

Processing studies were reviewed for potato, spinach, tomatoes, oranges, apple, plums, and 

cottonseed. Generally residues of cyantraniliprole and metabolites IN-J9Z38, IN-JCZ38, INK7H19, IN-MLA84, IN-MYX98, IN-N7B69, IN-N5M09, and IN-F6L99 were determined with 

an LOQ of 0.01 ppm for each. All field trials were conducted at an application rate of about 0.40 

lb ai/A (1X). This yielded quantifiable residues on all RACs, except one potato trial.

N

H

O

N NCH3

Br

O

NHCH3

N

Cl

NC

N

N

N NCH3

Br

O

CH3

N

Cl

NC

N

N

CH3

O

NC

Cl

HN N

Br

H3CNH

O

DPX-HGW86

IN-J9Z38

IN-N5MO9

IN-F6L99
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Table 129: Summary of Processing Factors for Cyantraniliprole

RAC Processed Commodity Processing Factor (Median)

Cyantraniliprole IN-J9Z38

Potato 

Flakes 0.6 1

Potato waste 0.6 1

Peeled potato 0.6 1

Chips 0.6 1

Wet peel 0.9 4

Culls 1 1

Fries 0.6 1

Unpeeled boiled 0.6 1

Unpeeled microwaved 0.6 1

Spinach Leaves, cooked 0.16 95

Tomato 

Washed 0.18 1

Peeled 0.08 1

Sundried 3.5 2.7

Canned 0.08 1

Juice 0.09 1

Wet pomace 0.65 1.7

Dry pomace 1.8 3.5

Paste 0.60 4.2

Puree 0.19 1.3

Orange

Juice 0.08 1

Wet Pulp 0.20 1

Dry Pulp 0.38 1

Meal 0.39 1

Molasses 0.08 8.9

Marmalade 0.08 1

Oil 6.2 7.5

Canned 0.077 1

Apple

Washed 0.57 1

Puree 1.1 1

Canned 0.12 1

Frozen 0.95 1

Juice 0.32 1

Wet pomace 1 1

Dry pomace 2.6 1.1

Applesauce 1.4 35

Plum Dried 1.5 1.4

Cotton

seed

Oil

(solvent ext) 0.03 1

Refined oil

(solvent ext) 0.006 1.5

Meal

(solvent ext) 0.04 1

Hull 0.33 0.89

Oil

(cold press) 0.27 0.89

Refined oil

(cold press) 0.004 1.6
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 107 of 193

Table 129: Summary of Processing Factors for Cyantraniliprole

RAC Processed Commodity Processing Factor (Median)

Cyantraniliprole IN-J9Z38

Meal

(cold press) 0.09 0.88

Conclusions. 

The processing studies were conducted according to commercial processes with RAC samples 

having quantifiable residues of cyantraniliprole, except one of three potato trial samples. The 

storage stability studies support the duration of storage of the RACs and processed fractions. 

The data indicate that residues of cyantraniprole concentrated in sundried tomato, dry tomato 

pomace, orange oil, dry apple pomace, apple puree, and dried plums. The submitted processing 

data are adequate for estimating residue levels in potential livestock feedstuffs and for dietary 

exposure analysis. For the latter, residue data were presented for metabolite IN-J9Z38. 

Tolerance calculations are presented in Appendix II. Processed commodities regulated in the US 

for which tolerances are considered include orange oil and dried plums. Note that a tolerance 

consideration is appropriate for wet apple pomace (1X), but not dry.

860.1650 Submittal of Analytical Reference Standards

Analytical standard for cyantraniliprole is currently not available in the EPA National Pesticide 

Standards Repository (personal communication with Theresa Cole, ACB ,01/02/13). 

Cyantraniliprole (5 g) and the Certificate of Analysis and the MSDS must be supplied. The 

reference standard should be sent to the Analytical Chemistry Lab, which is located at Fort 

Meade, Maryland, to the attention of either Theresa Cole or Thuy Nguyen at the following 

address:

USEPA

National Pesticide Standards Repository/Analytical Chemistry Branch/OPP

701 Mapes Road

Fort George G. Meade, MD 20755-5350

(Note that the mail will be returned if the extended zip code is not used.)

860.1850 Confined Accumulation in Rotational Crops

Monograph for Cyantraniliprole, Section B.7.9.1 (MRIDs 48119986 and 48119987)

DuPont submitted confined rotational crops studies with cyantraniliprole labeled in the cyano 

and pyrazole carbonyl groups. Two separate studies were submitted. A pilot study that included 

soybeans was not conducted under GLP as supplementary information since soybeans are often 

rotated in the field.

MRID 48119986: In the pilot study, spring wheat, soybeans, and red beets were grown in a 

greenhouse. A single treatment of [PC14C] at 300 g ai/ha was applied to a sandy loam soil in 

separate pots into which seeds of each crop were sown. Samples of wheat grain, chaff and straw, 

red beet foliage and roots, and soybean foliage, pods and beans were harvested 25 days and 120 
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 108 of 193

days after treatment (DAT). TRR (ppm) in various matrices and distributions of residues in 

wheat, red beet, and soybean are tabulated below.

Table 130. Total radioactive residues in food items sampled from crops sown at intervals 

following single 300 g ai/ha applications of [14C]-cyantraniliprole

DAT (days) Commodity

Wheat Soybeans Red Beet

Grain Chaff Straw Seed Foliage Pods Root Foliage

25 0.021 0.374 1.149 0.038 0.185 0.094 0.021 0.107

120 0.019 0.201 0.749 0.018 0.127 0.073 0.008 0.039

Levels of parent and various metabolites (IN-K7H19, IN-K5A77, IN-K5A78, IN-K5A79, INJ9Z38, IN-JCZ38, IN-JSE76, IN-MLA84, IN-N7B69) in wheat grain, soybean seed and red beet 

root were all each <0.01 ppm at 25 and 125 day PBI’s. Levels of parent and various metabolites 

in wheat chaff and straw, soybean pod and foliage, and red beet foliage each ranged from <0.01 

ppm to 0.52 ppm. 

MRID 48119987: The main study was conducted under GLP and the rotational crops included 

lettuce, red beet, and wheat. One 450 g ai/ha application of either [CN14C]-cyantraniliprole or 

[PC14C]-cyantraniliprole was made to bare soil. Seeds of spring wheat, lettuce, and red beet 

were sown 30 days and 120 days after treatment. After 365 days aging, a further sowing of 

spring wheat was made into the soil which had received the 30 days sowing. Lettuce and red beet

sowing were not conducted at 365 days due to low residues levels determined at earlier plantback intervals. TRR in various matrices and distributions of residues in wheat grain, beet root, 

and lettuce are tabulated in the tables below.

Table 131. Total radioactive residues in food commodities sampled from crops sown at intervals 

following single 450 g ai/ha applications of [14C]-cyantraniliprole

RAC

Interval 

(DATa)

[CN-14C]-cyantraniliprole [PC-14C]-cyantraniliprole

TRR (ppm) TRR (ppm)

Wheat grain

30 0.056 0.054

120 0.004 0.008

365 0.014 0.018

Lettuce 30 0.114 0.078

120 0.036 0.023

Red beet roots 30 0.021 0.031

120 0.007 0.009

a: DAT Days after soil treatment

TRR in various matrices and distributions of residues in wheat forage, hay and straw, and red 

beet foliage are tabulated below.

Table 132. Total radioactive residues in feed items sampled from crops sown at intervals 

following single 450 g ai/ha applications of [14C]-cyantraniliprole

RAC

Interval 

(DATa)

[CN14C]-cyantraniliprole [PC14C]-cyantraniliprole

TRR

ppm

TRR

ppm

Wheat forage

30 0.313 0.287

120 0.129 0.103

365 0.128 0.089
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Wheat hay

30 1.463 1.625

120 0.312 0.449

365 0.561 0.555

Wheat straw

30 0.973 0.966

120 0.347 0.272

365 0.429 0.482

Red beet 

foliage

30 0.063 0.145

120 0.013 0.045

a: DAT Days after soil treatment

For wheat grain, levels of parent ranged from 0.011 to 0.020 ppm at the 30 day PBI, and was 

<0.01 ppm at the 365 day PBI. For red beet root, levels of parent were <0.01 ppm at 30 and 120 

day PBI. For lettuce, levels of parent ranged from 0.009 to 0.079 ppm at 30 and 120 day PBI. 

Levels of metabolites in these edible crop parts were all <0.01 ppm at these PBIs. 

Levels of parent in wheat forage, hay and straw were all above 0.01ppm at all 3 PBIs except 

wheat forage (0.006 ppm) at the 365 day PBI. Levels of parent in beet foliage were all <0.01 

ppm at 30 and 120 day PBI. Levels of the metabolites were matrix dependent wheat feed stuffs 

and some (IN-J9Z38, IN-MLA84, IN-MYX98) were present at >0.01 ppm but most were 

predominantly below 0.01 ppm. Levels of metabolites in beet foliage were all <0.01 ppm.

Table 133: Nature of the residue in various commodities following single 450 g ai/ha (0.40 lb 

ai/acre) application of [14C] cyantraniliprole to soil

Identificati

on

[CN14C]-cyantraniliprole [PC14C]-cyantraniliprole

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

WHEAT GRAIN

30-DAT 365-DAT 30-DAT 365-DAT

Cyantranili

prole 20.0 0.011 33.0 0.005 36.3 0.020 13.6 0.003

IN-J9Z38 2.9 0.002 2.8 <0.001 5.1 0.003 ND

IN-JCZ38 5.7 0.003 ND 0.8 <0.001 ND

IN-K5A79 ND ND ND 3.4 0.001

IN-K7H19 ND ND ND 7.2 0.002

INMLA84 1.9 0.001 ND 1.2 0.001 ND

IN-N7B69 ND ND ND 5.2 0.001

BEET ROOTS

30-DAT 30-DAT

Cyantranili

prole

21.0 0.005 23.8 0.007

IN-DBC80 NA 4.1 <0.001

IN-J9Z38 3.2 <0.001 5.5 0.002

IN-K5A77 1.2 <0.001 2.2 <0.001

INMYX98ND ND 2.4 0.001

IN-N7B69 6.3 <0.001 2.9 <0.001

LETTUCE

30 DAT 120 DAT 30 DAT 120 DAT

Cyantranili

prole

68.1 0.079 69.1 0.025 60.0 0.047 39.6 0.009

IN-J9Z38 4.5 0.005 1.4 0.001 1.2 0.001 ND ND

IN-JCZ38 3.7 0.005 1.6 0.001 ND ND ND ND

IN- 4.3 0.005 2.3 0.001 3.5 0.003 2.1 <0.001
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 110 of 193

Table 133: Nature of the residue in various commodities following single 450 g ai/ha (0.40 lb 

ai/acre) application of [14C] cyantraniliprole to soil

Identificati

on

[CN14C]-cyantraniliprole [PC14C]-cyantraniliprole

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

MLA84

INMYX980.7 0.001 16.4 0.007 ND ND 9.1 0.003

WHEAT FORAGE

30 DAT 365 DAT 30 DAT 365 DAT

Cyantranili

prole 72.7 0.226 25.2 0.032 71.0 0.205 6.4 0.006

IN-J9Z38 4.0 0.013 3.9 0.005 3.8 0.011 ND

IN-JCZ38 1.8 0.006 6.2 0.008 1.6 0.004 ND

IN-JSE76 ND ND 0.2 0.001 1.2 0.001

INHGW87 ND ND 0.5 0.002 3.4 0.003

IN-K7H19 ND ND 1.4 0.005 1.2 0.001

IN-K5A79 ND ND ND 2.1 0.002

INMYX98 ND ND ND 1.5 0.001

INMLA84 3.9 0.012 6.8 0.008 5.0 0.015 ND

IN-N7B69 ND ND ND 4.4 0.004

WHEAT HAY

30 DAT 365 DAT 30 DAT 365 DAT

Cyantranili

prole 53.4 0.782 40.8 0.230 52.2 0.848 29.5 0.163

IN-DBC80 NA NA 1.5 0.025 1.5 0.008

INHGW87 ND 0.6 0.004 0.3 0.005 ND

IN-J9Z38 12.3 0.181 12.9 0.073 10.9 0.178 7.1 0.039

IN-JCZ38 2.3 0.033 3.7 0.021 2.7 0.044 4.0 0.022

IN-JSE76 ND 1.0 0.006 0.6 0.010 2.6 0.015

IN-K5A77 ND 0.8 0.004 ND 0.2 0.001

IN-K5A78 0.1 0.001 0.2 0.001 ND 0.3 0.001

IN-K5A79 ND 0.7 0.004 ND 0.2 0.001

IN-K7H19 ND 1.3 0.007 0.9 0.014 10.9 0.060

INMLA84 6.1 0.087 5.5 0.031 6.6 0.108 2.9 0.016

INMYX98 0.8 0.013 3.3 0.018 ND ND

IN-N7B69 1.3 0.019 1.6 0.010 0.3 0.005 0.7 0.004

IN-NBC94 ND 0.3 0.002 ND ND

WHEAT STRAW

30 DAT 365 DAT 30 DAT 365 DAT
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 111 of 193

Table 133: Nature of the residue in various commodities following single 450 g ai/ha (0.40 lb 

ai/acre) application of [14C] cyantraniliprole to soil

Identificati

on

[CN14C]-cyantraniliprole [PC14C]-cyantraniliprole

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

Cyantranili

prole

44.8 0.437 33.0 0.141 41.3 0.399 35.1 0.153

IN-DBC80 NA NA ND 1.8 0.008

INHGW87ND ND 0.6 0.006 ND

IN-J9Z38 10.9 0.108 8.9 0.038 9.5 0.091 8.1 0.035

IN-JCZ38 4.3 0.040 1.8 0.007 5.5 0.053 5.5 0.024

IN-JSE76 1.5 0.013 4.8 0.021 0.8 0.008 3.9 0.017

IN-K5A77 0.3 0.002 1.2 0.005 0.3 0.003 1.3 0.007

IN-K5A78 0.2 0.002 ND ND 0.4 0.002

IN-K5A79 1.6 0.015 1.5 0.007 ND ND

IN-K7H19 0.3 0.003 0.8 0.003 ND 10.1 0.043

INMLA845.8 0.056 5.1 0.022 5.5 0.053 4.5 0.019

INMYX981.2 0.012 ND ND 1.5 0.014 1.6 0.007

IN-N7B69 ND ND 4.2 0.018 ND ND

IN-NBC94 ND ND 0.5 0.004 0.1 0.001

GARDEN BEET FOLIAGE

30 DAT 120 DAT 30 DAT 120 DAT

Cyantranili

prole

3.0 0.003 2.1 <0.001 4.1 0.005 0.8 <0.001

IN-DBC80 NDd ND 1.7 0.002 ND

INHGW87ND ND 0.2 <0.001 ND

IN-J9Z38 ND ND ND 0.7 <0.001

IN-JCZ38 1.5 0.001 2.0 <0.001 1.8 0.003 ND

IN-JSE76 ND ND 1.0 0.001 3.8 0.002

IN-K5A77 ND ND 0.2 <0.001 ND

IN-K5A78 ND ND 0.6 <0.001 ND

IN-K5A79 1.1 0.001 ND 5.0 0.007 ND

IN-K7H19 0.7 <0.001 ND 3.2 0.006 ND

INMLA84ND ND 1.7 0.002 ND

INMYX98ND ND 0.5 <0.001 ND

IN-N7B69 0.7 <0.001 ND 1.4 0.002 ND

IN-NBC94 ND ND ND 0.7 <0.001
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 112 of 193

Figure 7: Proposed metabolic pathway for cyantraniliprole in confined rotational crops

Conclusions. The submitted studies are adequate to satisfy data requirements.

Cyantraniliprole was the main residue in wheat grain and lettuce, accounting for up to 0.02 and 

0.079 ppm, respectively. Metabolite IN-MYX98 was detected in lettuce at about 10% - 15% 

TRR, but generally metabolites were individually <10% TRR in food items. In soybean seed 

and beet root, TRR was low and no compound individually accounted for >0.01 ppm.

Transfer of cyanraniliprole and its metabolites to animal feed items was higher than observed in 

the commodities for human consumption. The TRR in animal feeds ranged from 0.09 to 1.62 

ppm at a 30 day PBI. The predominant residue in wheat chaff, forage, hay, and straw and 

soybean foliage was parent.

The main metabolite detected in wheat forage was IN-J9Z38, which accounted for more than 

10% of the TRR but less than 0.05 ppm.

The main metabolites detected in wheat hay, were IN-J9Z38, IN-K7H19 which accounted for 

more than 10% TRR and 0.05 ppm, even for 365 days-long PBI. IN-MLA84 was quantified 

above at concentrations 0.05 ppm, but did not account for more than 6.6 % TRR.

N

N

O

N

N N

Br

Cl

N

NH

N

O

N

N

N

Br

Cl

N

O

H2N O

HN

NH

O

N

N N

Cl

Br

NH

O

HN

O

N

N N

Cl

Br

O

HO

N

N N

NH

O Br

HN

O

HO

N

Cl

N

N N

NH

O

Cl

Br

HN

O

N

NH

O

N N

N

Br

Cl

N

H

O OH

N

N

N N

N

H

O

NH2

O

Br

Cl

O

H2N

N

N

O

N

N N

Br

Cl

HO

O

N

N N

O

Br

Cl

HO

N

N

O

N

N N

Br

Cl

HO

O

NH

O

N N

N

Br

Cl

NH2

O

N

NH

N

O

N

N N

Br

Cl

N

HO

NH

O

N N

N

Br

Cl

N

O OH

N

HO

N

N N

NH

O Br

HN

O

GluO

N

Cl

NH

O

NH2

O

N

N N

Cl

Br

O

HO

N

N

O

N

N N

Br

Cl

N

HO

IN-N7B69

IN-MYX98

IN-J9Z38

IN-MLA84

IN-JCZ38

IN-JSE76

IN-K7H19

IN-K5A77

IN-DBC80

IN-K5A78

Cyantraniliprole

(DPX-HGW86)

IN-HGW87

Hydroxy IN-MLA84

Bis-hydroxy Cyantraniliprole

IN-K5A79

IN-NBC94

IN-N7B69 Glucoside
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The main metabolites detected in wheat straw, were IN-J9Z38, IN-K7H19, IN-MLA84, INJCZ38 and IN-JSE76, which accounted for more than 0.05 ppm. Each of these components was 

at least three times less abundant than the parent, which represented 22.9 to 44.8% TRR.

The main metabolites detected in soybean foliage, were IN-MLA84 and IN-JSE76, which 

accounted for more than 10 %TRR. None of these components was quantified above 0.05 ppm. 

The main metabolite detected in soybean pods was IN-JCZ38, which accounted for more than 

10 %TRR, but was not quantified above 0.05 ppm.

IN-N7B69 glucoside conjugate (0.02-0.05 ppm) was the principal component in red beet foliage, 

whereas parent was not quantified in this commodity. 

The metabolic pathway is similar to that for primary crops. In wheat, lettuce, red beets, and 

soybeans the cyano group underwent varying degrees of hydrolysis to amide and carboxylic acid 

(IN-K7H19, IN-JCZ38, IN-JSE76, IN-K5A77, IN-K5A78, and IN-K5A79) moieties. 

Cyantraniliprole also cyclized and was dehydrated both with and without loss of the N-methyl 

group to form IN-J9Z38 and IN-MLA84 (the demethylated metabolite of IN-J9Z38). 

Demethylation, presumably via oxidation of the N-methyl group to a hydroxymethyl group (as in 

IN-MYX98) then oxidation to the carboxylic acid and eventual loss of carbon dioxide was also a 

pathway leading to IN-HGW87, the free amide of parent. Oxidation on cyantraniliprole’s 

benzylic carbon gave the hydroxymethyl metabolite IN-N7B69. Glucose conjugation of INN7B69 was also observed. Hydroxylated IN-MLA84 and bis hydroxy cyantraniliprole were also 

reported. IN-DBC80, formed by cleavage of the carboxamide linkage between the pyridine and 

phenyl rings, could be formed from parent or a number of metabolites.

The residue of concern in rotational crops for tolerance enforcement and risk assessment is 

cyantraniliprole (ROCKS decision memo, D404411, 9/25/2012). Based on the results of the 

confined rotational crop studies (see 860.1900 below), field rotational crop studies are needed for 

cyantraniliprole.

860.1900 Field Accumulation in Rotational Crops

Monograph for Cyantraniliprole, Section B.7.9.2 (MRIDs 48119995, 48119996, 48120005, 48120035, 48519713)

DuPont submitted field rotational crop data from five studies conducted in North America. 

MRID 48119996: In two plots (120-day soybean and oat restart plots), bare soil was treated with 

two applications of cyantraniliprole 100 g/L OD in the 2006/2007 growing season. In all other 

plots, bare soil was treated with three applications of cyantraniliprole 100 g/L OD. The seasonal 

rate was 450 g ai/ha/season. Three applications were made at 150 g ai/ha in all but two plots. 

Two applications (150 g ai/ha for Application 1, and 300 g ai/ha for Application 2) were made to 

the 120-day soybean and oat restart plots. Due to unfavorable weather conditions, applications 2 

and 3 were combined by doubling the amount of test substance. No test substance was applied to 

control plots.

Four (rotational) crops were subsequently planted in the treated and untreated soil at the 

indicated intervals after the last application (plant-back intervals). 

The only residue found on lettuce, root top, root, and oat grain was cyantraniliprole, which 

declined to <LOQ (0.01 ppm) at a 120 day PBI for lettuce, at 14 days for oat grain, radish root, 
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radish top, and soybean seed. Significant residues of metabolites IN-J9Z38, IN-JCZ38, and INMLA894 were found on the livestock feed commodities oat forage, oat hay, oat straw, soybean 

forage, and soybean hay at 14 – 120 day PBIs. The levels are low, 0.01 – 0.06 ppm per 

metabolite, and the total contribution per commodity would be no more than 0.1 ppm at 14 – 30 

day PBIs. 

Table 134: Maximum Residues of Cyantraniliprole and Metabolites (IN-J9Z38, IN-JCZ38, and INMLA84) from Rotational Crop Field Trials Following Treatment with Cyantraniliprole (100 g/L OD) 

in the United States (NAFTA Region 11) in 2006-2007

Crop Part Cyantraniliprole IN-J9Z38 IN-JCZ98 IN-MLA84

Max PBI PBI* Max PBI PBI* Max PBI PBI* Max PBI PBI*

ppm days days ppm days days ppm days days ppm days days

Lettuce Im

mature 0.039 14 120 <0.01 14 14 <0.01 14 14 <0.01 14 14

Mature 0.033 14 120 <0.01 14 14 <0.01 14 14 <0.01 14 14

Oat Grain <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.036 14 120 <0.01 14 14 0.019 30 365 <0.01 14 14

Hay 0.13 14 365 0.035 14 120 0.056 30 365 <0.01 14 14

Straw 0.045 14 365 0.029 14 365 0.029 120 365 <0.01 14 14

Radish Root <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Top 0.046 14 120 <0.01 14 14 <0.01 14 14 <0.01 14 14

Soybean Seed <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.18 14 365 <0.01 14 14 0.025 120 365 0.023 14 30

Hay 0.023 14 365 <0.01 14 14 0.037 30 365 0.012 14 120

* when residues <LOQ

MRID 48119995: In a second study, bare soil was treated with three applications of 

Cyantraniliprole 100 g/L OD, 3 applications at 150 g ai/ha per application, 5-day retreatment 

interval. No test substance was applied to control plots. Four (rotational) crops were 

subsequently planted in the treated and untreated soil at plant-back intervals (PBIs) of 14, 30, 

120, and 365 days. In cereals grain, radish root, soybean seed, spinach leaves, no residue above 

0.01 ppm was quantified in any sample from each plant-back interval, except in grain from oats 

sown 14 and 30 DAT, in which up to 0.01 ppm were quantified.

For livestock feed items, in radish tops no single component was quantified above 0.01 ppm, 

except cyantraniliprole in tops from plants sown 14 DAT (0.018 ppm). Low amounts of INJ9Z38 only were quantified in tops from plants sown 120 DAT. In spring and winter forage, hay 

and straw from 14 and 30 days PBI, cyantraniliprole was the main compound. In hay and straw 

samples from 14 and 30 days PBI, sum of IN-J9Z38 and IN-JCZ38 was similar to the amount of 

cyantraniliprole. In 120 days PBI straw sample, IN-J9Z38 was the only compound quantified 

above 0.01 ppm. 
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Table 135: Maximum Residues of Cyantraniliprole and Metabolites (IN-J9Z38, IN-JCZ38, and INMLA84) from Rotational Crop Field Trials Following Treatment with Cyantraniliprole (100 g/L OD) 

in the United States (NAFTA Region 10) in 2006-2007.

Crop Part Cyantraniliprole IN-J9Z38 IN-JCZ98 IN-MLA84

Max PBI PBI* Max PBI PBI* Max PBI PBI* Max PBI PBI*

ppm days days ppm days days ppm days days ppm days days

Spinach Immature <0.01 30 30 <0.01 14 14 <0.01 14 14 <0.01 14 14

Mature <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Oat Grain 0.010 30 30 <0.01 14 14 <0.01 14 14 <0.01 14 14

Spring 

forage 0.018 14 119 <0.01 14 14 <0.01 14 14 <0.01 14 14

Winter 

forage 0.022 30 119 <0.01 30 30 0.010 30 30 <0.01 14 14

Hay 0.096 30 119 0.021 30 119 0.048 30 119 <0.01 14 14

Straw 0.073 30 119 0.033 30 365 0.029 30 119 <0.01 14 14

Radish Root <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Top 0.019 14 31 <0.01 14 14 <0.01 14 14 <0.01 14 14

Soybean Seed <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.043 14 119 <0.01 14 14 0.015 31 119 0.015 14 31

Hay 0.100 14 119 0.023 14 31 0.054 14 119 0.062 14 119

* when residues <LOQ

MRID 48120035: A study was conducted in Ontario, Canada, during the 2008–2009 growing 

seasons. Bare soil was treated with three applications of cyantraniliprole 100 g/L OD, total 

application 450 g ai/ha. Four (rotational) crops (lettuce, radish, oats, soybean) were subsequently 

planted in the treated and untreated soil at the targeted plant-back intervals (PBIs) of 14, 30, 120, 

and 365 days.

Regarding commodities for human consumption (cereals grain, radish root, soybean seed, lettuce 

leaves), no residue above 0.01 ppm was quantified in any sample from 365 days plant-back 

interval. Cyantraniliprole was the only compound quantified above 0.01 ppm in immature and 

mature leaves of lettuce from 16 and 29 days PBI. No residue was quantified in samples from 

366 days PBI, and no samples were analyzed between 29 and 365 day PBIs. Cyantraniliprole and 

IN-J9Z38 were quantified in radish roots from 30 and 120 days PBI. Residues of all metabolites 

were less than LOQ at the 16-day plantback interval. No residue was quantified in samples from 

366 days PBI. In cereal grains, no residue above 0.01 ppm was quantified in any sample.

For livestock feed commodities, in oat forage and hay, maximum residues of cyantraniliprole 

were 0.011 ppm, and 0.034 ppm, respectively at the 14-day plantback interval. The only 

metabolites detected above the LOQ were IN-J9Z38 and IN-JCZ98 in oat hay at 0.011 ppm and 

0.012 ppm, respectively at the 14-day PBI. In oat straw, residues of the parent compound and 

metabolites were at or less than 0.01 ppm at the 14-day PBI. In tops of radish, cyantraniliprole 

was quantified at 0.039 ppm at the 16-day PBI. Cyantraniliprole was quantified above 0.01 ppm

in samples from 14, 29 and 121 days PBI. IN-J9Z38 and INJCZ38 were quantified at lower 

levels (ca 0.005 ppm). In soybean forage and hay, only cyantraniliprole was quantified at the 15-

day PBI with maximum levels of 0.023 ppm, and 0.061 ppm. Levels declined to less than 0.01 

ppm at 29 days PBI (forage) and 366 days PBI (hay).
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Table 136: Maximum Residues of Cyantraniliprole and Metabolites (IN-J9Z38, IN-JCZ38, INMLA84) from Rotational Crop Field Trials Following Treatment with Cyantraniliprole (100 g/L) in 

Canada (NAFTA Region 5) in 2008-2009

Crop

Part Cyantraniliprole IN-J9Z38 IN-JCZ98 IN-MLA84

Max PBI PBI* Max PBI PBI* Max PBI PBI* Max PBI PBI*

ppm days days ppm days days ppm days days ppm days days

Lettuce Immature 0.026 16 366 <0.01 16 16 <0.01 16 16 <0.01 16 16

Mature 0.018 29 366 <0.01 16 16 <0.01 16 16 <0.01 16 16

Oat Grain <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.011 14 31 <0.01 14 <0.01 <0.01 14 14 <0.01 14 14

Hay 0.034 14 365 0.011 14 31 0.012 14 31 <0.01 14 14

Straw 0.021 14 31 0.016 14 188 <0.01 14 14 <0.01 14 14

Radish Root 0.015 29 121 <0.01 16 16 <0.01 16 16 <0.01 16 16

Top 0.039 16 121 <0.01 16 16 <0.01 16 16 <0.01 16 16

Soybean Seed <0.01 15 15 <0.01 15 15 <0.01 15 15 <0.01 15 15

Forage 0.023 15 29 <0.01 15 15 <0.01 15 15 <0.01 15 15

Hay 0.061 15 366 <0.01 15 15 0.010 15 15 0.010 15 15

* when residues <LOQ

MRID 48120035: A study was conducted in Georgia, USA; Missouri, USA; Ontario, Canada; 

California, USA; and Washington, USA in the 2009/2010 growing season. Bare soil was treated 

with three broadcast applications of cyantraniliprole 100 g/L SE, for a total application rate of 

450 g ai/ha (0.40 lb ai/acre). At each trial location, five to twelve (rotational) crops were 

subsequently planted at a targeted PBI of 30 days. Crops were turnip, sugar beet, garden beet, 

carrot, bean, pea, soybean, field corn, sweet corn, sorghum, rice, wheat, Bermuda grass, alfalfa, 

bromegrass, clover, and bluegrass.

The maximum residue of cyantraniliprole was quantified in carrot root (0.011 ppm), but not in 

other root vegetables. No residue of cyantraniliprole and metabolites (IN-J9Z38, IN-JCZ98, INMLA84) was detected in seeds (soybean, pea and bean).

For livestock feed items, residues of cyantraniliprole ranged from <0.01 to 0.012 ppm in tops 

from root crops and stover from field corn, sweet corn and sorghum, with the maximum residue 

observed in field corn stover. The only metabolite quantified in stover was IN-J9Z38 in field 

corn and sweet corn stover at levels up to 0.013 ppm.

In forage from wheat, alfalfa, bluegrass and soybean, residues of cyantraniliprole, IN-J9Z38, INJCZ38, and IN-MLA84 ranged from <0.01-0.11 ppm, <0.01-0.011 ppm, <0.01 ppm, and <0.01-

0.020 ppm, respectively. The highest residue was observed in wheat forage. In hay from wheat, 

alfalfa, bluegrass, bean, pea, and soybean, residues of cyantraniliprole ranged from 0.024 to 0.63 

ppm with the highest residue observed in sorghum hay. The residues of IN-J9Z38, IN-JCZ38, 

and IN-MLA84 in hay ranged from <0.01-0.028 ppm, <0.01-0.029 ppm, and <0.01-0.069 ppm, 

respectively.
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Table 137: Maximum Residue Data from Rotational Crop Field Trials Following Treatment with 

Cyantraniliprole (100 g/L SE) in the United States and Canada in 2009.

Commodity PBI

(days)

Cyantraniliprole IN-J9Z38 IN-JCZ38 IN-MLA84

(ppm) (ppm) (ppm) (ppm)

Turnip top 30 <0.01 <0.01 <0.01 <0.01

Turnip root 30 <0.01 <0.01 <0.01 <0.01

Sugar beet top 30 <0.01 <0.01 <0.01 <0.01

Sugar beet root 30 <0.01 <0.01 <0.01 <0.01

Garden beet top 30 <0.01 <0.01 <0.01 <0.01

Garden beet root 30 <0.01 <0.01 <0.01 <0.01

Carrot root 30 0.011 <0.01 <0.01 <0.01

Bean forage 30 0.029 <0.01 <0.01 <0.01

Bean hay 30 0.140 0.014 <0.01 0.017

Bean succulent seed 30 <0.01 <0.01 <0.01 <0.01

Bean dry seed 30 <0.01 <0.01 <0.01 <0.01

Bean seed 30 <0.01 <0.01 <0.01 <0.01

Pea forage 30 0.035 <0.01 <0.01 <0.01

Pea hay 30 0.13 0.010 0.016 <0.01

Pea succulent seed 30 <0.01 <0.01 <0.01 <0.01

Pea dry seed 30 <0.01 <0.01 <0.01 <0.01

Pea seed 30 <0.01 <0.01 <0.01 <0.01

Soybean forage 30 0.091 <0.01 <0.01 0.014

Soybean hay 30 0.63 0.012 0.029 0.069

Soybean seed 30 <0.01 <0.01 <0.01 <0.01

Field corn forage 30 <0.01 <0.01 <0.01 <0.01

Field corn stover 30 0.012 0.012 <0.01 <0.01

Sweet corn forage 30 <0.01 <0.01 <0.01 <0.01

Sweet corn stover 30 0.010 0.013 <0.01 <0.01

Sorghum forage 30 0.010 <0.01 <0.01 <0.01

Sorghum stover 30 <0.01 <0.01 <0.01 <0.01

Rice straw 30 <0.01 <0.01 <0.01 <0.01

Wheat forage 30 0.11 <0.01 <0.01 <0.01

Wheat hay 30 0.21 0.017 <0.01 0.012

Wheat straw 30 0.081 0.012 0.015 0.012

Bermuda grass forage 30–31 0.011 <0.01 <0.01 <0.01

Bermuda grass hay 30–31 0.025 <0.01 0.010 <0.01

Bromegrass forage 30–31 0.011 <0.01 <0.01 <0.01

Bromegrass hay 30–31 0.024 <0.01 <0.01 <0.01

Alfalfa forage 30–31 0.054 <0.01 <0.01 0.020

Alfalfa hay 30–31 0.14 0.020 0.011 0.051

Clover forage 30–31 <0.01 0.011 <0.01 <0.01

Clover hay 30–31 0.034 0.028 0.019 0.012

Bluegrass forage 30 0.092 <0.01 <0.01 <0.01

Bluegrass hay 30 0.23 0.028 <0.01 <0.01

MRID 48519713: In another study, bare soil was treated with three bare ground broadcast 

applications of cyantraniliprole 100 g/L SE with adjuvants added to the spray mixtures. The total 

application rate was 450 g ai/ha.Ten (rotational) crops were subsequently planted in the treated 

and untreated soil at 30 1 day targeted intervals after the last application (plant-back intervals). 

The crops were turnip, sugar beet, radish, carrot, pea, soybean, alfalfa, clover, peanut, and 

strawberries.

No residue of metabolites IN-K7H19, IN-MYX98 or IN-N7B69 was detected in any sample. IN-
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J9Z38 was found in carrot root at 0.01 ppm. For livestock feed commodities, in clover hay, 

soybean hay, turnip tops, soybean forage, alfalfa hay, and clover forage, cyantraniliprole was 

quantified at 0.046 ppm, 0.19 ppm, 0.020 ppm, 0.039 ppm, 0.021 ppm, 0.023 ppm, respectively. 

Metabolites were quantifiable in soybean hay and clover hay.

Table 139. Maximum Residues of Cyantraniliprole and Metabolites (IN-J9Z38, IN-JCZ38, and 

IN-MLA84) from Rotational Crop Following Treatment with Cyantraniliprole (100 g/L SE) in 

the United States and Canada (NAFTA Region 1, 2, 5, 6, 7, 8, 9, 10, 11, and 14) in 2010.

Commodity PBI

(days)

Cyantraniliprole IN-J9Z38 IN-JCZ38 IN-MLA84

ppm ppm ppm ppm

Carrot root 30-32 <0.01 0.010 <0.01 <0.01

Radish root 29-31 <0.01 <0.01 <0.01 <0.01

Sugar beet root 29-33 <0.01 <0.01 <0.01 <0.01

Sugar beet top 29-33 <0.01 <0.01 <0.01 <0.01

Turnip top 30 0.020 <0.01 <0.01 <0.01

Pea forage 30 <0.01 <0.01 <0.01 <0.01

Pea hay 30 <0.01 0.017 <0.01 <0.01

Pea seed 30 <0.01 <0.01 <0.01 <0.01

Soybean forage 29-36 0.039 <0.01 <0.01 <0.01

Soybean hay 29-36 0.19 0.021 0.026 0.025

Soybean pod 29-36 <0.01 <0.01 <0.01 <0.01

Soybean seed 29-36 <0.01 <0.01 <0.01 <0.01

Alfalfa forage 29-33 <0.01 <0.01 <0.01 <0.01

Alfalfa hay 29-33 0.021 <0.01 <0.01 <0.01

Clover forage 29-36 0.023 <0.01 0.013 <0.01

Clover hay 29-36 0.046 0.013 0.034 0.011

Peanut hay 29-35 <0.01 0.015 <0.01 <0.01

Peanut nutmeat 29-35 <0.01 <0.01 <0.01 <0.01

Strawberry berry 29-31 <0.01 <0.01 <0.01 <0.01

Conclusions:

Rotational field trials were conducted for numerous livestock feed commodities and for feed and 

food root type commodities (including tops). Generally no residues were found at a 365 day 

PBI, but the petitioner proposed a 30 day PBI. The trials were conducted with a 30 day PBI and 

with cyantranilirprole applied to the soil at an approximate 1X maximum seasonal rate (0.4 lb 

ai/acre). 

The number and distribution of trials for each commodity meet the requirements of OCSPP 

860.1500, with the exception of cereal grain forages/fodders/straws (Crop Group 16). See 

Appendix III. A total of 42 trials are suggested, but only 16 were supplied. This is acceptable, 

as residues were not found on any grain, and the concern is livestock feed items only. The trials 

were widely distributed across corn (field and sweet), wheat, oats, sorghum, and rice. 

The trials are adequate to establish inadvertent tolerances for crop subgroup 1A (based on radish 

root), crop group 2 (based on radish top), crop group 7 (based on soybean hay), crop group 16 

(based on wheat hay), crop group 17 (based on grass hay), crop group 18 (based on alfalfa hay), 

and peanut hay. In such cases, the PBI is 30 days. See Appendix II for details. 

860.1550 Proposed Tolerances
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DuPont has proposed that the residue of concern for tolerance expression in crop commodities is 

cyantraniliprole, that the residue of concern for tolerance expression in livestock commodities is 

cyantraniliprole, and that the residue of concern for tolerance expression in processed plant 

commodities is the sum of cyantarniliprole and metabolite IN-J9Z38. HED has concluded 

(ROCKS decision memo, D404411, 9/25/2012) that the residue of concern for tolerance 

enforcement in plant commodities, processed plant commodities, and livestock commodities is 

cyantraniliprole.

The tolerances proposed are listed in Table 140 along with the tolerance levels recommended by 

HED. The Organization for Economic Cooperation and Development (OECD) tolerance 

statistical calculation procedures were utilized for determining appropriate tolerance levels for 

raw agricultural crop commodities, both primary crop and rotational crop; see Appendix II for 

tolerance calculations. 

Adequate field trial data are available to support the proposed uses on Brassica (cole) Leafy 

Vegetables (Group 5), Bulb Vegetables (Group 3-07), Bushberries (Group 13-07B), Oilseed 

(Group 20), Citrus Fruit (Group 10-10), Cucurbit Vegetables (Group 9), Fruiting Vegetables 

(Group 8-10), Leafy Vegetables (non-Brassica) (Group 4), Pome Fruit (Group 11-10), Stone 

Fruit (Group 12-12), Tree Nuts (Group 14), and Tuberous and Corm Vegetables (Subgroup 1C). 

In some cases the proposed tolerance value (see Table 140 and Appendix II) needs to be 

changed, based on the application of the OECD statistical calculation procedure to the validated 

field trial data.

Seed treatment of canola indicates that seed treatment plus foliar applications yields residues less 

than those from foliar application only, where the total seasonal rate is 0.40 lb ai/acre. This is 

supplemented by numerous commodity trials where at-plant treatment plus foliar applications do 

not exceed residues from foliar treatments only, where the total seasonal rate is 0.40 lb ai/acre. 

However, this situation does not apply to root and tuber crops where seed treatment plus foliar 

application leads to higher residues than foliar treatment only, where the total seasonal rate in 

each case is 0.40 lb ai/acre. The canola seed treatment data may be extrapolated to seed treatment 

for sunflower seed and mustard seed under the same treatement rates.

There are no data to support use in Food/Feed Handling Establishments.

The proposed tolerances for almond hulls, Brassica leafy vegetables head and stem (crop 

subgroup 5A), cattle liver, citrus raw peel, cucurbit vegetables (crop group 9), leafy vegetables 

except Brassica (crop group 4), oilseeds (crop group 20), pome fruits (crop group 11-10), potato 

wet peel, stone fruits except cherry (crop group 20), and tree nuts (crop group 14), need to be 

modified. The recommended primary plant commodity tolerances are derived from use of the 

Organization of Economic Cooperation and Development (OECD) statistical calculation 

procedure. With the exception of the proposed tolerance for leaves of root and tuber vegetables, 

the proposed tolerances for inadvertent residues, with a PBI of 30 days, need to be revised to 

reflect the recommended tolerances based on the use of the OECD statistical calculation 

procedure. These calculation procedures explain the differences observed in proposed and 

recommended tolerances. The calculations are detailed in Appendix II. . Also, for some crop 

groups, more than one tolerance was proposed for various components of the group, for example, 

crop group 6 (forage, fodder, and straw of cereal grains). Only one tolerance is possible per 

group, and the tolerance is based on the member commodity with the highest residue levels.
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The proposed tolerances for processed commodities are not appropriate for citrus raw peel and 

potato wet peel. The processing studies do not show a concentration of residue in these 

commodities relative to the RAC. The proposed tolerance for citrus oil needs to be lowered from 

6 ppm to 2.4 ppm, consistent with the results from application of the median processing factor to 

the HAFT from field trials (orange). 

The proposed tolerances for livestock commodities are acceptable, except the proposals for liver

of cattle and hog and sheep and goat and horse, hog fat, hot meat, hog meat byproducts, and milk 

fat. Evaluation of the feeding study and use of the maximum reasonably balance livestock diets

indicate that a tolerance value of 0.01 ppm is appropriate for liver, to be covered the meat 

byproducts’ tolerance. The dietary exposures of hogs and poultry indicate no need for tolerances

(no residue anticipated), and there is no indication of significant concentration of cyantraniliprole 

in milk fat relative to milk.

Currently there are no Codex MRLs/tolerances for cyantraniliprole. Therefore, there are no 

issues with harmonization of tolerances. Canadian MRLs are being proposed concurrently with 

US tolerances and are harmonized for primary crop commodities and livestock commodities 

except fruiting vegetables. For fruiting vegetables, the US has a greenhouse use not registered 

in Canada, and this use necessitates a higher tolerance than the proposed Canada MRL. The US 

tolerance for fruiting vegetables is harmonized with the European Union. Also, Canada will 

establish import tolerances for grapes and olives. The US does not have this request. Canada 

does not establish MRLs for livestock only commodities, and therefore there exists disharmony. 

Finally, Canada must set MRLs for poultry commodities at the LOQ of the analytical method 

(0.01 ppm). This is in accord with Canadian policy for situations of no residues anticipated in 

the livestock commodities where there are feed items with residues. The US is not establishing 

tolerances for poultry commodities as no significant accumulation in poultry tissues or eggs is 

expected, i.e., category 40CFR §180.6(a)(3). 

The recommended tolerances for plant and livestock commodities are presented in Table 140. 

Table 140. Tolerance Summary for Cyantraniliprole.

Commodity Proposed Tolerance 

(ppm)

Recommended/ 

Harmonized

Tolerance (ppm)

Comments; Correct Commodity 

Definition

(a) It is recommended that tolerances be established for residues of the insecticide cyantraniliprole (3-bromo-1-(3-

chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide), 

including its metabolites and degradates, in or on the commodities below. Compliance with the tolerance levels 

specified below is to be determined by measuring only cyantraniliprole in or on the commodity.

Almond, hulls 30 8.0 A revised Section F must be 

submitted to propose a tolerance 

at 8 ppm

Berries and small fruits, 

bushberries (crop subgroup 13-

07B)

4 4.0 Bushberry subgroup 13-07B
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Table 140. Tolerance Summary for Cyantraniliprole.

Commodity Proposed Tolerance 

(ppm)

Recommended/ 

Harmonized

Tolerance (ppm)

Comments; Correct Commodity 

Definition

Brassica (cole) leafy 

vegetables, head and stem 

Brassica (crop subgroup 5A)

2 3.0 Brassica, head and stem, 

subgroup 5A

A revised Section F must be 

submitted to propose a tolerance 

at 3 ppm

Brassica (cole) leafy 

vegetables, leafy Brassica

greens (crop subgroup 5B)

30 30 Brassica, leafy greens, subgroup 

5B

Bulb vegetables, onion, bulb 

(crop subgroup 3-07A)

0.04 0.04 Onion, bulb, subgroup 3-07A

Bulb vegetables, onion, green 

(crop subgroup 3-07B)

8 8.0 Onion, green, subgroup 3-07B

Cattle, fat 0.01 0.01

Cattle, liver 0.04 None Included under meat byproducts

Cattle, meat 0.01 0.01

Cattle, meat byproducts, except 

liver

0.01 0.01 Cattle, meat byproducts

Cherries 6 6.0 Cherry subgroup 12-12A

Citrus fruits (crop group 10-

10)

0.7 0.70 Fruit, citrus, group 10-10

Citrus, oil 4 2.4 A revised Section F must be 

submitted to propose a tolerance 

at 2.4 ppm.

Citrus, raw peel 0.9 None Covered by citrus tolernace 

Cotton, gin byproduct 10 10 Cotton, gin byproducts

Cucurbit vegetables (crop 

group 9)

0.3 0.40 A revised Section F must be 

submitted to propose a tolerance 

at 0.4 ppm.

Vegetable, cucurbit, group 9

Fruiting vegetables (crop 

group 8-10)

2 2.0 Vegetable, fruiting, group 8-10

Goat, fat 0.01 0.01

Goat, liver 0.04 None Included under meat byproducts

Goat, meat 0.01 0.01

Goat, meat byproducts, except 

liver

0.01 0.01 Goat, meat byproducts

Hog, fat 0.01 None 40CFR§180(a)(3)

Hog, liver 0.04 None 40CFR§180(a)(3)

Hog, meat 0.01 None 40CFR§180(a)(3)

Hog, meat byproducts, except 

liver

0.01 None 40CFR§180(a)(3)

Horse, fat 0.01 0.01

Horse, liver 0.04 None Included under meat byproducts

Horse, meat 0.01 0.01
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Table 140. Tolerance Summary for Cyantraniliprole.

Commodity Proposed Tolerance 

(ppm)

Recommended/ 

Harmonized

Tolerance (ppm)

Comments; Correct Commodity 

Definition

Horse, meat byproducts, except 

liver

0.01 0.01 Horse, meat byproducts.

Leafy vegetables (except 

Brassica vegetables) (crop 

group 4)

15 20 A revised Section F must be 

submitted to propose a tolerance 

at 20 ppm.

Vegetable, leafy, except 

Brassica, group 4

Milk 0.01 0.01

Milk fat 0.04 None Covered by milk tolerance

Oilseeds, except cotton 

byproduct (crop group 20)

1 1.5 A revised Section F must be 

submitted to propose a tolerance 

at 1.5 ppm.

Oilseed group 20

Pome fruits (crop group 11-10) 0.8 1.5 A revised Section F must be 

submitted to propose a tolerance 

at 1.5 ppm.

Fruit, pome, group 11-10

Potato, wet peel 0.3 None Covered under subgroup 1C 

tolerance

Root and tuber vegetables, 

tuberous and corm vegetables 

(crop subgroup 1C)

0.15 0.15 Vegetable, tuberous and corm, 

subgroup 1C

Sheep, fat 0.01 0.01

Sheep, liver 0.04 None Included under meat byproducts 

Sheep, meat 0.01 0.01

Sheep, meat byproducts, 

except liver

0.01 0.01 Sheep meat byproducts

Stone fruits, except cherries 

(crop group 12)

1.5 1.5 Peach subgroup 12-12B

0.50 Plum subgroup 12-12C

Tree nuts, except almond hulls 

(crop group 14)

0.06 0.04 A revised Section F must be 

submitted to propose a tolerance 

at 0.04 ppm.

Nut, tree, group 14

(d) It is recommended that indirect or inadvertent tolerances be established for residues of the insecticide 

cyantraniliprole (3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1Hpyrazole-5-carboxamide), including its metabolites and degradates, in or on the commodities below. Compliance 

with the tolerance levels specified below is to be determined by measuring only cyantraniliprole in or on the 

commodity.

Foliage of legume vegetables 

(crop group 7), forage

0.15 None Only one tolerance per crop 

group.

Foliage of legume vegetables 

(crop group 7), hay

0.6 0.70 A revised Section F must be 

submitted to propose a tolerance 

at 0.7 ppm.

Vegetable, foliage of legume, 

group 7

Forage, fodder and straw of 

cereal grains (crop group 16), 

forage

0.06 None Only one tolerance per crop 

group.
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Table 140. Tolerance Summary for Cyantraniliprole.

Commodity Proposed Tolerance 

(ppm)

Recommended/ 

Harmonized

Tolerance (ppm)

Comments; Correct Commodity 

Definition

Forage, fodder and straw of 

cereal grains (crop group 16), 

hay and straw

0.15 0.50 A revised Section F must be 

submitted to propose a tolerance 

at 0.5 ppm.

Grain, cereal, forage, fodder and 

straw, group 16

Grass forage, fodder, and hay 

(crop group 17), forage

0.06 None Only one tolerance per crop 

group.

Grass forage, fodder, and hay 

(crop group 17), hay

0.15 0.50 A revised Section F must be 

submitted to propose a tolerance 

at 0.5 ppm.

Grass, forage, fodder and hay, 

group 17

Leaves of root and tuber 

vegetables (human food or 

animal feed) (crop group 2)

0.04 0.04 Vegetable, leaves of root and 

tuber, group 2

Nongrass animal feeds (forage, 

fodder, straw, and hay) (crop 

group 18), forage

0.06 None Only one tolerance per crop 

group.

Nongrass animal feeds (forage, 

fodder, straw, and hay) (crop 

group 18), hay

0.15 0.20 A revised Section F must be 

submitted to propose a tolerance 

at 0.2 ppm.

Animal feed, nongrass, group 18

Peanut, hay 0.03 0.01 A revised Section F must be 

submitted to propose a tolerance 

at 0.01 ppm.

Peanut, hay

Root and tuber vegetables, root 

vegetables (crop subgroup 1A)

0.03 0.02 A revised Section F must be 

submitted to propose a tolerance 

at 0.02 ppm.

Vegetable, root, subgroup 1A

Attachments: 

International Residue Limits

Appendix I – Summary of Cyantraniliprole and Metabolites

Appendix II - Tolerance Assessment Calculations

Template Version September 2005
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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International Residue Limits 

Cyantraniliprole (090098; 12/31/2012)

Summary of US and International Tolerances and Maximum Residue Limits 

Residue Definition:

US Canada Mexico2 Codex3

40 CFR 180.XXX:

Plants: Tolerances are established for 

residues of the insecticide cyantraniliprole (3-

bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano2-methyl-6-[(methylamino)carbonyl]phenyl]-

1H-pyrazole-5-carboxamide), including its 

metabolites and degradates, in or on the 

commodities below. Compliance with the 

tolerance levels specified below is to be 

determined by measuring only 

cyantraniliprole in or on the commodity.

Livestock: Tolerances are established for 

residues of the insecticide cyantraniliprole (3-

bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano2-methyl-6-[(methylamino)carbonyl]phenyl]-

1H-pyrazole-5-carboxamide), including its 

metabolites and degradates, in or on the 

commodities below. Compliance with the 

tolerance levels specified below is to be 

determined by measuring only 

cyantraniliprole in or on the commodity.

Identical 

to US

None

Commodity1 Tolerance (ppm) /Maximum Residue Limit (ppm)

US Canada Mexico2 Codex3

Almond, hulls 8.0 8

Bushberry subgroup 13-07B 4.0 4

Brassica, head and stem, subgroup 

5A

3.0 3

Brassica, leafy greens, subgroup B 30 30

Onion, bulb, subgroup 3-07A 0.04 0.04

Onion, green, subgroup 3-07B 8.0 8

Cattle fat 0.01 0.01

Cattle, meat 0.01 0.01

Cattle, meat byproducts 0.01 0.01

Cherry subgroup 12-12B 6.0 6 (cherry)

Fruit, citrus, group 10-10 0.7 0.7

Citrus, oil 2.4 2.4

Cotton, gin byproducts 10 10

Vegetable, cucurbit, group 9 0.4 0.4

Vegetable, fruiting, group 8-10 2.0 0.7

Goat, fat 0.01 0.01

Goat, meat 0.01 0.01

Goat, meat byproducts 0.01 0.01

Horse, fat 0.01 0.01

Horse, meat 0.01 0.01

Horse, meat byproducts 0.01 0.01
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Summary of US and International Tolerances and Maximum Residue Limits 

Residue Definition:

US Canada Mexico2 Codex3

Vegetable, leafy, except Brassica,

group 4

20 20

Milk 0.01 0.01

Oilseed group 20 1.5 1.5

Fruit, pome, group 11-10 1.5 1.5

Vegetable, tuberous and corm, 

subgroup 1C

0.15 0.15

Sheep, fat 0.01 0.01

Sheep, meat 0.01 0.01

Sheep, meat byproducts 0.01 0.01

Peach subgroup 12-12A 1.5 1.5

(stone 

fruit 

except 

cherry)

Plum subgroup 12-12C 0.5

Nut, tree, group 14 0.04 0.04

Inadvertent Tolerances

Vegetable, foliage of legume, group 

7

0.7 N/A4

Grain, cereal, forage, fodder and 

straw, group 16

0.5 N/A

Grass, forage, fodder and hay, group 

17

0.5 N/A

Vegetable, leaves of root and tuber, 

group 2

0.04 0.04

Animal feed, nongrass, group 18 0.2 N/A

Peanut, hay 0.01 N/A

Vegetable, root, subgroup 1A 0.02 0.02

1

Includes only commodities of interest for this action. Tolerance values should be the HED recommendations and 

not those proposed by the applicant.

2 Mexico adopts US tolerances and/or Codex MRLs for its export purposes.

3 * = absent at the limit of quantitation; Po = postharvest treatment, such as treatment of stored grains. PoP = 

processed postharvest treated commodity, such as processing of treated stored wheat. (fat) = to be measured on the 

fat portion of the sample. MRLs indicated as proposed have not been finalized by the CCPR and the CAC.

4 Canada does not establish tolerances for livestock feed commodities. Canada, by law, must include MRLs for 

poultry at the LOQ. Also, Canada is establishing import MRLs for olives and grapes as per request of DuPont. 

These MRLs are harmonized with the EU.
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APPENDIX I: Structures of Major Cyantraniliprole Metabolites

Common Name Chemical Name Structure

Cyantraniliprole

SYN545377

CYANTRANILIPROLE

CAS Name: 3-Bromo-1-(3-chloro-2-

pyridinyl)-N-[4-cyano-2-methyl-6 

[(methylamino)carbonyl]phenyl]-1Hpyrazole-5-carboxamide

IN-MLA84 CAS Name: 2-[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-yl]-1,4-dihydro-8-

methyl-4-oxo-6-quinazolinecarbonitrile N

NH

N

N

O

Br

N

Cl

CH3

N

IN-N7B69 CAS Name: 3-Bromo-1-(3-chloro-2-

pyridinyl)-N-[4-cyano-2-(hydroxymethyl)-

6-[(methylamino)carbonyl]phenyl]-1Hpyrazole-5-carbonitrile

Br

Cl

O

O

NH

N

N

HN

N

N

CH3

HO

IN-MYX98 CAS Name: 3-Bromo-1-(3-chloro-2-

pyridinyl)-N-[4-cyano-2-[[(hydroxymethyl) 

amino]carbonyl]-6-[(methylphenyl]-1Hpyrazole-5-carboxamide

Br

Cl

O

O

NH

N

N

NH

N

N

CH3

HO

IN-J9Z38 CAS Name: 2-[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-yl]-3,4-dihydro3,8-dimethyl-4-oxo-6-

quinazolinecarbonitrile

IN-NXX69 CAS Name: 2-[[(4Z)-2-bromo-4Hpyrazolo[1,5-d]pyrido[3,2-b][1,4]oxazin-4-

ylidene]amino]-5-cyano-N,3-

dimethylbenzamide

N

N

N

N

H

O

Cl

Br

HN O

N

N

N

O

N

N

N

Br

Cl

N

N

N

N

N

O

Br

HN O

N
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Common Name Chemical Name Structure

IN-QKV54 CAS Name: 2-(3-Bromo-1H-pyrazol-5-yl)-

1,4-dihydro-3,8-dimethyl-4-oxo-6-

quinazolinecarbonitrile

IN-RNU71 CAS Name: 2-(2-Bromo-4-

oxopyrazolo[1,5-a]pyrido[3,2-e]pyrazin5(4H)-yl)-5-cyano-N,3-dimethylbenzamide

IN-JSE76 CAS Name: 4-[[[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-

yl]carbonyl]amino]-3-methyl-5-

[(methylamino)carbonyl]benzoic acid

IN-JCZ38 CAS Name: 4-[[[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-

yl]carbonyl]amino]-N'3',5-dimethyl-1,3-

benzenedicarboxamide

IN-K5A78 CAS Name: 2-[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-yl]-3,4-dihydro3,8-dimethyl-4-oxo-6-quinazolinecarboxylic 

acid

IN-K5A77 CAS Name: 2-[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-yl]-3,4-dihydro3,8-dimethyl-4-oxo-6-

quinazolinecarboxamide

IN-K5A79 CAS Name: 3-(Aminocarbonyl)-4-[[[3-

bromo-1-(3-chloro-2-pyridinyl)-1H-pyrazol5-yl]carbonyl]amino]-5-methylbenzoic acid

N

N

O

N N

H

Br

N

O

HN

N

N

N N

O Br

N

N

H

O

O

N

N

N

Cl

Br

O

OH

HN

O

NH2

HN O

N

H

O

N

N

N

Cl

Br

OH

O

N

N

O

N

N

N

Br

Cl

H2N

O

N

N

O

N

N

N

Br

Cl

N

H

H2N O

O

N

N

N

Cl

Br

O

HO
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Common Name Chemical Name Structure

IN-PLT97 CAS Name: 2-[3-Bromo-1-(3-chloro-2-

pyridinyl)-1H-pyrazol-5-yl]-1,4-dihydro-8-

methyl-4-oxo-6-quinazolinecarboxylic acid

HO

O N

N

H

N

N

O

N

Br

Cl
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Appendix II: TOLERANCE ASSESSMENT CALCULATIONS

MRL Calculations for Raw Agricultural Commodities (Primary Crops and 

Rotational Crops) and Processed Commodities

Tolerance estimates for primary crops and rotational crops were derived using the field trial data 

and the Organization of Economic Cooperation and Development (OECD) calculation 

procedure. Details are provided below for each crop/crop group or subgroup.

Tolerances for Raw Agricultural Commodities from Primary Crop Field Trials

Tuberous and Corm vegetables (subgroup 1C)

The tolerance estimate for subgroup 1C is 0.15 ppm, based on potato seed treatment plus foliar 

treatment..

Potato

One seed treatment plus one late season foliar treatment.

Cyantraniliprole

Potato

NAFTA

Seed treatment FS (13.5 g ai/100 kg seed) + 1 Foliar OD 

(0.13 – 0.14 lb ai/acre), 7 day PHI

Total number of data (n) 21

Percentage of censored data 33%

Number of non-censored data 14

Lowest residue 0.010

Highest residue 0.110

Median residue 0.014

Mean 0.025

Standard deviation (SD) 0.025

Correction factor for censoring (CF) 0.778

Proposed MRL estimate

- Highest residue 0.110

- Mean + 4 SD 0.125

- CF x 3 Mean 0.057

Unrounded MRL 0.125

Rounded MRL 0.15
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Residues (mg/kg) n

< 0.01 7

0.01 2

0.011 1

0.014 2

0.02 1

0.023 2

0.027 1

0.028 1

0.031 1

0.052 1

0.072 1

0.11 1

Three late season foliar applications

Cyantraniliprole

Potato

USA

3 foliar OD

Total number of data (n) 19

Percentage of censored data 79%

Number of non-censored data 4

Lowest residue 0.010

Highest residue 0.034

Median residue 0.010

Mean 0.012

Standard deviation (SD) 0.006

Correction factor for censoring (CF) 0.481

Proposed MRL estimate

- Highest residue 0.034

- Mean + 4 SD 0.037

- CF x 3 Mean 0.018

Unrounded MRL 0.037

Rounded MRL 0.04

High uncertainty of MRL estimate.
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[High level of censoring]

Residues (mg/kg) n

< 0.01 15

0.011 1

0.018 1

0.02 1

0.034 1

3 foliar SE

Cyantraniliprole

Potato

NAFTA

SE, 3X0.13, 7 d PHI

Total number of data (n) 5

Percentage of censored data 60%

Number of non-censored data 2

Lowest residue 0.010

Highest residue 0.030

Median residue 0.010

Mean 0.016

Standard deviation (SD) 0.009

Correction factor for censoring (CF) 0.600

Proposed MRL estimate

- Highest residue 0.030

- Mean + 4 SD 0.052

- CF x 3 Mean 0.029

Unrounded MRL 0.052

Rounded MRL 0.06

High uncertainty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 3

0.021 1

0.03 1
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Bulb vegetables (group 3-07)

The tolerance estimate for subgroup 3-07A, based on bulb onion, is 0.04 ppm. The tolerance 

estimate for subgroup 3-07B is 8 ppm, based on green onion. A group tolerance is not possible, 

as the high and low estimates differ by more than 5X.

Dry bulb onion

Cyantraniliprole

Bulb onion, dry

USA

Foliar 0.40 lb ai/A

Total number of data (n) 10

Percentage of censored data 40%

Number of non-censored data 6

Lowest residue 0.010

Highest residue 0.027

Median residue 0.014

Mean 0.015

Standard deviation (SD) 0.006

Correction factor for censoring (CF) 0.733

Proposed MRL estimate

- Highest residue 0.027

- Mean + 4 SD 0.038

- CF x 3 Mean 0.033

Unrounded MRL 0.038

Rounded MRL 0.04

Residues (mg/kg) n

< 0.01 4

0.012 1

0.015 1

0.016 1

0.019 1

0.021 1

0.027 1

Green onion

Cyantraniliprole

Green onion

USA
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Foliar 0.4 lb ai/A

Total number of data (n) 5

Percentage of censored data 0%

Number of non-censored data 5

Lowest residue 0.380

Highest residue 4.100

Median residue 1.300

Mean 1.602

Standard deviation (SD) 1.481

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 4.100

- Mean + 4 SD 7.525

- CF x 3 Mean 4.806

Unrounded MRL 7.525

Rounded MRL 8

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.38 1

0.63 1

1.3 1

1.6 1

4.1 1

Leafy Vegetables (group 4)

The tolerance estimate for the leafy vegetables group 4, based on spinach, is 20 ppm. A group 

tolerance is possible because the difference between the low tolerance estimate (head lettuce 5 

ppm) and the high tolerance estimate (spinach 20 ppm) is <5X.

Head Lettuce

Cyantraniliprole

Head Lettuce

NAFTA

OD: 3X0.13, 1 d PHI

Total number of data (n) 12

Percentage of censored data 0%

Number of non-censored data 12

Lowest residue 0.018

Highest residue 2.700
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Median residue 0.695

Mean 0.955

Standard deviation (SD) 0.855

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 2.700

- Mean + 4 SD 4.375

- CF x 3 Mean 2.866

Unrounded MRL 4.375

Rounded MRL 5

Residues (mg/kg) n

0.018 1

0.084 1

0.16 1

0.18 1

0.53 1

0.64 1

0.75 1

1.3 1

1.6 1

1.7 1

1.8 1

2.7 1

Head Lettuce

1 X 200 g/L SC soil + 2 X 100 g/L OD foliar, 1 day PHI.

One trial. 0.017 ppm HAFT. Insufficient data.

Head Lettuce

Cyantraniliprole

Head Lettuce

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non-censored data 6

Lowest residue 0.130

Highest residue 2.100
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Median residue 0.665

Mean 0.872

Standard deviation (SD) 0.786

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 2.100

- Mean + 4 SD 4.014

- CF x 3 Mean 2.615

Unrounded MRL 4.014

Rounded MRL 4

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.13 1

0.17 1

0.5 1

0.83 1

1.5 1

2.1 1

Head lettuce

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day. One trial.

Insufficient data, 0.9 ppm HAFT.

Leaf Lettuce

Cyantraniliprole

Leaf Lettuce

USA

OD, 3X0.13, 1 d PHI

Total number of data (n) 12

Percentage of censored data 0%

Number of non-censored data 12

Lowest residue 1.100

Highest residue 6.800

Median residue 2.650

Mean 3.117

Standard deviation (SD) 1.693

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 6.800

- Mean + 4 SD 9.890
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- CF x 3 Mean 9.350

Unrounded MRL 9.890

Rounded MRL 10

Residues (mg/kg) n

1.1 1

1.2 1

1.9 1

2.1 1

2.4 2

2.9 1

3.3 1

3.9 1

4 1

5.4 1

6.8 1

Leaf Lettuce

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

One trial. 1.1 ppm HAFT, Insufficient data.

Leaf Lettuce

Cyantraniliprole

Leaf Lettuce

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non-censored data 6

Lowest residue 2.400

Highest residue 6.800

Median residue 4.250

Mean 4.433

Standard deviation (SD) 1.981

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 6.800

- Mean + 4 SD 12.356

- CF x 3 Mean 13.300

Unrounded MRL 13.300

Rounded MRL 15
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High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

2.4 1

2.5 1

3.2 1

5.3 1

6.4 1

6.8 1

Leaf lettuce

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day

Insufficient data, 1.7 ppm.

Celery

Cyantraniliprole

Celery

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non-censored data 11

Lowest residue 0.280

Highest residue 9.100

Median residue 2.000

Mean 2.783

Standard deviation (SD) 2.684

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 9.100

- Mean + 4 SD 13.517

- CF x 3 Mean 8.348

Unrounded MRL 13.517

Rounded MRL 15

Residues (mg/kg) n

0.28 1

0.73 1

1 1

1.1 1
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1.2 1

2 1

2.3 1

2.5 1

4.7 1

5.7 1

9.1 1

Cyantraniliprole

Celery

NAFTA

OD, 2X0.13, 1 day PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non-censored data 6

Lowest residue 0.680

Highest residue 5.500

Median residue 1.600

Mean 2.550

Standard deviation (SD) 2.137

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 5.500

- Mean + 4 SD 11.100

- CF x 3 Mean 7.650

Unrounded MRL 11.100

Rounded MRL 15

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.68 1

0.92 1

1.3 1

1.9 1

5 1

5.5 1

Celery

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

One trial. 3.5 ppm HAFT, Insufficient data.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 139 of 193

Celery

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day

One trial, 3.5 ppm HAFT, Insufficient data.

Spinach

Cyantraniliprole

Spinach

NAFTA

OD: 3X0.13, 1 d PHI

Total number of data (n) 7

Percentage of censored data 0%

Number of non-censored data 7

Lowest residue 3.800

Highest residue 13.000

Median residue 4.600

Mean 6.243

Standard deviation (SD) 3.345

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 13.000

- Mean + 4 SD 19.621

- CF x 3 Mean 18.729

Unrounded MRL 19.621

Rounded MRL 20

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

3.8 1

4.1 1

4.2 1

4.6 1

5.8 1

8.2 1

13 1

Spinach

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

One trial, 6.70 ppm HAFT, insufficient data.

Spinach

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day

One trial, 6.80 ppm HAFT, insufficient data.

The greatest tolerance estimate, 20 ppm from 3 foliar applications of the OD formulation to spinach, is the 

appropriate value. It varies by 5X from the lowest estimate, 4 ppm for head lettuce from three foliar applications of 

the SE formulation to head lettuce.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Brassica Leafy Vegetables (group 5)

The tolerance estimates are 2 ppm for head cabbage, 3 ppm for broccoli/cauliflower, and 30 ppm 

for mustard greens. As the estimates vary by a factor of more than 5X from low to high, a group 

tolerance is not possible. Based on the head cabbage and broccoli/cauliflower results, a tolerance 

of 3 ppm is appropriate for head and stem Brassica vegetables, subgroup 5A. Based on the 

mustard green results, a tolerance of 30 ppm is appropriate for Brassica leafy greens, subgroup 

5B.

Cabbage

Cyantraniliprole

Head Cabbage

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non-censored data 11

Lowest residue 0.290

Highest residue 0.950

Median residue 0.560

Mean 0.556

Standard deviation (SD) 0.221

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.950

- Mean + 4 SD 1.440

- CF x 3 Mean 1.669

Unrounded MRL 1.669

Rounded MRL 2

Residues (mg/kg) n

0.29 1

0.32 2

0.42 1

0.47 1

0.56 1

0.57 1

0.65 1

0.71 1

0.86 1

0.95 1

Head Cabbage
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 141 of 193

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

1 Trial, 0.49 ppm HAFT, insufficient data.

Mustard Greens

Cyantraniliprole

Mustard Greens

NAFTA

OD, 3X-0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non-censored data 11

Lowest residue 2.400

Highest residue 19.000

Median residue 6.000

Mean 7.391

Standard deviation (SD) 4.773

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 19.000

- Mean + 4 SD 26.482

- CF x 3 Mean 22.173

Unrounded MRL 26.482

Rounded MRL 30

Residues (mg/kg) n

2.4 1

3.4 1

3.9 1

5.5 1

5.8 1

6 1

7.1 1

7.2 1

8 1

13 1

19 1

Mustard Greens

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

1 Trial, 3.1 ppm HAFT, insufficient data.

Broccoli/Cauliflower
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Cyantraniliprole

Broccoli/Cauliflower

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 10

Percentage of censored data 20%

Number of non-censored data 8

Lowest residue 0.010

Highest residue 1.000

Median residue 0.530

Mean 0.468

Standard deviation (SD) 0.333

Correction factor for censoring (CF) 0.867

Proposed MRL estimate

- Highest residue 1.000

- Mean + 4 SD 1.799

- CF x 3 Mean 1.217

Unrounded MRL 1.799

Rounded MRL 2

Residues (mg/kg) n

< 0.01 2

0.22 1

0.28 1

0.51 1

0.55 1

0.59 1

0.69 1

0.82 1

1 1

Cyantraniliprole

Broccoli/Cauliflower

NAFTA

SE, 3X0.13, 1 d PHI

Total number of data (n) 4

Percentage of censored data 0%

Number of non-censored data 4

Lowest residue 0.470

Highest residue 1.100

Median residue 0.700

Mean 0.743
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 143 of 193

Standard deviation (SD) 0.272

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 1.100

- Mean + 4 SD 1.830

- CF x 3 Mean 2.228

Unrounded MRL 2.228

Rounded MRL 3

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.47 1

0.61 1

0.79 1

1.1 1

Broccoli/Cauliflower

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

1 Trial, 0.48 ppm HAFT, insufficient data.

Fruiting vegetables (group 8-10)

An appropriate tolerance for the fruiting vegetable group is 1.5 ppm, based on the highest 

estimate from the foliar treatments of peppers with the OD formulation in greenhouses. Note 

that the greenhouse data are from Europe.

Tomato (field trials)

Cyantraniliprole

Tomato

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 20

Percentage of censored data 0%

Number of non-censored data 20

Lowest residue 0.041

Highest residue 0.260

Median residue 0.080

Mean 0.099

Standard deviation (SD) 0.053

Correction factor for censoring (CF) 1.000
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Proposed MRL estimate

- Highest residue 0.260

- Mean + 4 SD 0.311

- CF x 3 Mean 0.297

Unrounded MRL 0.311

Rounded MRL 0.4

Residues (mg/kg) n

0.041 1

0.046 1

0.058 2

0.065 1

0.067 1

0.071 1

0.072 1

0.076 1

0.077 1

0.082 1

0.086 1

0.093 1

0.1 1

0.12 1

0.14 2

0.16 1

0.17 1

0.26 1

Pepper (field trials)

Cyantraniliprole

Bell Pepper

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non-censored data 11

Lowest residue 0.032

Highest residue 0.280

Median residue 0.075

Mean 0.119

Standard deviation (SD) 0.086

Correction factor for censoring (CF) 1.000
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Proposed MRL estimate

- Highest residue 0.280

- Mean + 4 SD 0.465

- CF x 3 Mean 0.358

Unrounded MRL 0.465

Rounded MRL 0.5

Residues (mg/kg) n

0.032 1

0.044 1

0.058 1

0.067 1

0.074 1

0.075 1

0.082 1

0.15 1

0.21 1

0.24 1

0.28 1

Cyantraniliprole

Non-Bell Pepper

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 9

Percentage of censored data 0%

Number of non-censored data 9

Lowest residue 0.068

Highest residue 0.420

Median residue 0.180

Mean 0.186

Standard deviation (SD) 0.123

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.420

- Mean + 4 SD 0.679

- CF x 3 Mean 0.557

Unrounded MRL 0.679

Rounded MRL 0.7
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Residues (mg/kg) n

0.068 1

0.07 1

0.079 1

0.095 1

0.18 1

0.2 1

0.25 1

0.31 1

0.42 1

Bell Peppers

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.092 ppm HAFT, insufficient data.

Non-Bell Peppers

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.15 ppm HAFT, insufficient data.

Tomatoes

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.048 ppm HAFT, insufficient data.
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Tomato and Pepper in Greenhouse in Europe

Cyantraniliprole Cyantraniliprole Cyantraniliprole Cyantrniliprole Cyantraniliprole

Tomato 

(Greenhouse)

Tomato 

(Greenhouse) Bell Pepper (GH)

Pepper 

(Greenhouse)

Non-Bell Pepper 

(GH)

Europe Europe Europe Europe Europe

OD, 4X0.107, 

foliar SC, 4X0.089, drip

OD, 4X0.107, 

foliar SC, 4X0.089, drip

OD, 4X0.107, 

foliar

14 8 9 14 5

0% 88% 0% 93% 0%

14 1 9 1 5

0.043 0.010 0.076 0.010 0.086

0.620 0.010 0.170 0.011 1.000

0.165 0.010 0.130 0.010 0.170

0.239 0.010 0.130 0.010 0.343

0.191 0.000 0.030 0.000 0.380

1.000 0.417 1.000 0.381 1.000

0.620 0.010 0.170 0.011 1.000

1.002 0.010 0.250 0.011 1.863

0.717 0.013 0.391 0.012 1.030

1.002 0.013 0.391 0.012 1.863

1 0.015 0.4 0.015 2
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High uncertainty 

of MRL estimate 

due to high level 

of censoring.

High uncertainty 

of MRL estimate 

due to high level 

of censoring.

High uncertainty 

of MRL estimate 

due to small 

dataset.

Residues (mg/kg) Residues (mg/kg) Residues (mg/kg) Residues (mg/kg) Residues (mg/kg)

0.043 0.010 * 0.130 0.010 * 0.120

0.180 0.010 * 0.140 0.010 * 1.000

0.140 0.010 * 0.120 0.010 * 0.086

0.140 0.010 * 0.096 0.010 * 0.340

0.072 0.010 * 0.076 0.010 * 0.170

0.180 0.010 * 0.160 0.010 *

0.150 0.010 * 0.130 0.010 *

0.140 0.010 0.150 0.010 *

0.053 0.170 0.010 *

0.220 0.010 *

0.620 0.010 *

0.420 0.010 *

0.590 0.010 *

0.400 0.011
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

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Cucurbit vegetables (group 9)

An appropriate tolerance for the cucurbit vegetable group 9 is 0.4 ppm, based on the highest 

estimate (melon) from the 3 foliar treatments with the OD formulation. The estimates were 0.3 

ppm for cucumber, 0.4 ppm for melon, and 0.2 ppm for summer squash, all from 3 foliar 

applications of the OD formulation at a total rate of about 0.4 lb ai/acre.

Cucumber

Cyantraniliprole

Cucumber

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 10

Percentage of censored data 0%

Number of non-censored data 10

Lowest residue 0.018

Highest residue 0.160

Median residue 0.043

Mean 0.057

Standard deviation (SD) 0.047

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.160

- Mean + 4 SD 0.246

- CF x 3 Mean 0.172

Unrounded MRL 0.246

Rounded MRL 0.3

Residues (mg/kg) n

0.018 1

0.021 1

0.023 1

0.029 1

0.041 1

0.045 2

0.07 1

0.12 1

0.16 1

Cyantraniliprole

Melon

NAFTA

OD, 3X0.13, 1 d PHI
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Total number of data (n) 9

Percentage of censored data 0%

Number of non-censored data 9

Lowest residue 0.056

Highest residue 0.185

Median residue 0.105

Mean 0.110

Standard deviation (SD) 0.043

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.185

- Mean + 4 SD 0.284

- CF x 3 Mean 0.331

Unrounded MRL 0.331

Rounded MRL 0.4

Residues (mg/kg) n

0.056 1

0.063 1

0.076 1

0.101 1

0.105 1

0.12 1

0.127 1

0.161 1

0.185 1

Cyantraniliprole

Summer Squash

NAFTA

OD, 3X0.13, 1d PHI

Total number of data (n) 9

Percentage of censored data 0%

Number of non-censored data 9

Lowest residue 0.011

Highest residue 0.110

Median residue 0.059

Mean 0.061

Standard deviation (SD) 0.030

Correction factor for censoring (CF) 1.000
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Proposed MRL estimate

- Highest residue 0.110

- Mean + 4 SD 0.181

- CF x 3 Mean 0.183

Unrounded MRL 0.183

Rounded MRL 0.2

Residues (mg/kg) n

0.011 1

0.034 1

0.043 1

0.053 1

0.059 1

0.072 1

0.08 1

0.088 1

0.11 1

Summer Squash

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.030 ppm HAFT, insufficient data.

Melon:

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.037 ppm HAFT, insufficient data.

Cucumber:

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.089 ppm HAFT, insufficient data.
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Citrus fruit (group 10-10)

The tolerance estimates are 0.5 ppm for grapefruit, 0.6 ppm for lemon, and 0.7 ppm for orange. 

The appropriate tolerance for the citrus group is 0.7 ppm.

Orange

Cyantrniliprole

Orange

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 13

Percentage of censored data 0%

Number of non-censored data 13

Lowest residue 0.098

Highest residue 0.390

Median residue 0.200

Mean 0.209

Standard deviation (SD) 0.096

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.390

- Mean + 4 SD 0.591

- CF x 3 Mean 0.627

Unrounded MRL 0.627

Rounded MRL 0.7

Residues (mg/kg) n

0.098 1

0.1 1

0.12 1

0.13 1

0.15 1

0.17 1

0.2 1

0.21 1

0.22 1

0.28 1

0.3 1

0.35 1

0.39 1
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Lemon

Cyantraniliprole

Lemon

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non-censored data 6

Lowest residue 0.160

Highest residue 0.300

Median residue 0.180

Mean 0.198

Standard deviation (SD) 0.053

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.300

- Mean + 4 SD 0.412

- CF x 3 Mean 0.595

Unrounded MRL 0.595

Rounded MRL 0.6

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.16 2

0.17 1

0.19 1

0.21 1

0.3 1

Grapefruit

Cyantraniliprole

Grapefruit

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 7

Percentage of censored data 0%

Number of non-censored data 7

Lowest residue 0.080

Highest residue 0.310

Median residue 0.140
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Page 154 of 193

Mean 0.157

Standard deviation (SD) 0.075

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.310

- Mean + 4 SD 0.457

- CF x 3 Mean 0.471

Unrounded MRL 0.471

Rounded MRL 0.5

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.08 1

0.12 2

0.14 2

0.19 1

0.31 1

Pome fruit (group 11-10)

The tolerance for the pome fruit group 11-10 is 1.5 ppm, based on the pear data at a 3 day PHI. 

The 7 day PHI data were not used for tolerance determination, in accordance with the proposed 

label.

Apple

Cyantraniliprole

Apple

NAFTA

SE, 3X0.13, 7 d PHI

Total number of data (n) 16

Percentage of censored data 0%

Number of non-censored data 16

Lowest residue 0.059

Highest residue 0.300

Median residue 0.125

Mean 0.142

Standard deviation (SD) 0.073

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.300
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- Mean + 4 SD 0.434

- CF x 3 Mean 0.426

Unrounded MRL 0.434

Rounded MRL 0.5

Residues (mg/kg) n

0.059 1

0.064 1

0.072 1

0.096 1

0.099 1

0.1 1

0.11 1

0.12 1

0.13 2

0.14 1

0.15 1

0.18 1

0.25 1

0.27 1

0.3 1

Cyantraniliprole

Apple

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 16

Percentage of censored data 0%

Number of non-censored data 16

Lowest residue 0.052

Highest residue 0.310

Median residue 0.158

Mean 0.168

Standard deviation (SD) 0.078

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.310
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- Mean + 4 SD 0.480

- CF x 3 Mean 0.505

Unrounded MRL 0.505

Rounded MRL 0.5

Residues (mg/kg) n

0.12 1

0.071 1

0.12 1

0.26 2

0.20 1

0.31 1

0.18 1

0.29 1

0.15 1

0.067 1

0.16 2

0.052 1

0.17 1

0.13 1

Pear

Cyantraniliprole

Pear

NAFTA

SE, 3X0.13, 7 d PHI

Total number of data (n) 10

Percentage of censored data 0%

Number of non-censored data 10

Lowest residue 0.042

Highest residue 0.560

Median residue 0.160

Mean 0.239

Standard deviation (SD) 0.181

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.560

- Mean + 4 SD 0.963

- CF x 3 Mean 0.716

Unrounded MRL 0.963
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Rounded MRL 1

Residues (mg/kg) n

0.042 1

0.065 1

0.1 1

0.12 1

0.16 2

0.35 1

0.38 1

0.45 1

0.56 1

Cyantraniliprole

Pear

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 10

Percentage of censored data 0%

Number of non-censored data 10

Lowest residue 0.076

Highest residue 0.580

Median residue 0.188

Mean 0.278

Standard deviation (SD) 0.194

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.580

- Mean + 4 SD 1.055

- CF x 3 Mean 0.833

Unrounded MRL 1.055

Rounded MRL 1.5

Residues (mg/kg) n

0.095 1

0.23 1

0.076 1

0.54 1

0.15 2

0.42 1
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0.14 1

0.12 1

0.44 1

0.58 1

Stone fruit (group 12-12)

The recommended tolerances are 0.5 ppm for plum, 1.5 ppm for peach, and 6 ppm for cherry. 

The difference between the low and high estimate is >5X. The difference between the estimates 

for a small stone fruit (cherry) and a large stone fruit (peach) is >5X. Therefore a stone fruit 

group tolerance is not possible, but tolerances may be set for each of the subgroups: cherry 

subgroup 12-12A at 6 ppm, plum subgroup 12-12C at 0.5 ppm, and peach subgroup 12-12B at 

1.5 ppm. 

Peach

Cyantraniliprole

peach

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 13

Percentage of censored data 0%

Number of non-censored data 13

Lowest residue 0.170

Highest residue 0.960

Median residue 0.340

Mean 0.386

Standard deviation (SD) 0.224

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.960

- Mean + 4 SD 1.281

- CF x 3 Mean 1.158

Unrounded MRL 1.281

Rounded MRL 1.5

Residues (mg/kg) n

0.17 1

0.19 2

0.23 1

0.25 1

0.27 1

0.34 1
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0.35 1

0.42 1

0.49 1

0.56 1

0.6 1

0.96 1

Plums

Cyantraniliprole

Plums

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 9

Percentage of censored data 0%

Number of non-censored data 9

Lowest residue 0.031

Highest residue 0.280

Median residue 0.066

Mean 0.104

Standard deviation (SD) 0.084

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.280

- Mean + 4 SD 0.441

- CF x 3 Mean 0.313

Unrounded MRL 0.441

Rounded MRL 0.5

Residues (mg/kg) n

0.031 1

0.044 1

0.052 1

0.064 1

0.066 1

0.071 1

0.13 1

0.2 1

0.28 1

Cherry

Cyantraniliprole

Cherry (sweet/tart)
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NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 7

Percentage of censored data 0%

Number of non-censored data 7

Lowest residue 0.330

Highest residue 3.800

Median residue 0.930

Mean 1.179

Standard deviation (SD) 1.190

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 3.800

- Mean + 4 SD 5.938

- CF x 3 Mean 3.536

Unrounded MRL 5.938

Rounded MRL 6

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.33 1

0.36 1

0.89 1

0.93 1

0.96 1

0.98 1

3.8 1

Blueberry (subgroup 13-07B)

The tolerance estimate for bushberry subgroup 13-07B is 4 ppm, based on 

blueberry field trial data.

Cyantraniliprole

blueberry

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 8

Percentage of censored data 0%

Number of non-censored data 8

Lowest residue 0.420

Highest residue 2.000
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Median residue 0.675

Mean 0.888

Standard deviation (SD) 0.563

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 2.000

- Mean + 4 SD 3.139

- CF x 3 Mean 2.663

Unrounded MRL 3.139

Rounded MRL 4

Residues (mg/kg) n

0.42 1

0.51 1

0.52 1

0.6 1

0.75 1

0.8 1

1.5 1

2 1

Tree Nuts (group 14)

The tolerance estimate for tree nuts is 0.04 ppm, based on the almond field trial data. The 

tolerance estimate for almond hulls is 8 ppm.

Almond

Cyantrniliprole

Almond nutmeat

USA

OS, 3X0.13, 5 d PHI

Total number of data (n) 6

Percentage of censored data 67%

Number of non-censored data 2

Lowest residue 0.010

Highest residue 0.023

Median residue 0.010

Mean 0.013

Standard deviation (SD) 0.005

Correction factor for censoring (CF) 0.556

Proposed MRL estimate

- Highest residue 0.023
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 162 of 193

- Mean + 4 SD 0.033

- CF x 3 Mean 0.021

Unrounded MRL 0.033

Rounded MRL 0.04

High uncertainty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 4

0.012 1

0.023 1

Almond Nutmeat

SE, 3X0.13, 5 d PHI

2 trials: 0.007, 0.018 ppm. Insufficient data for estimate. Residues do not exceed the tolerance estimate for the OD 

formulation.

Cyantraniliprole

Almond hulls

USA

OD, 3X0.13, 5 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non-censored data 6

Lowest residue 0.880

Highest residue 4.600

Median residue 1.600

Mean 2.082

Standard deviation (SD) 1.447

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 4.600

- Mean + 4 SD 7.871

- CF x 3 Mean 6.245

Unrounded MRL 7.871

Rounded MRL 8

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.88 1

0.91 1

1.3 1

1.9 1

2.9 1
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 163 of 193

4.6 1

Almond Hulls

Two trials, SE formulation, 3X0.13 lb ai/acre, 5 day PHI

2.5 ppm and 3.6 ppm are below the tolerance estimate (8 ppm) for us of the OD formulation.

Cyantraniliprole

Pecan nutmeat

USA

OD, 3X0.13, 5 d PHI

Total number of data (n) 6

Percentage of censored data 100%

Number of non-censored data 0

Lowest residue 0.010

Highest residue 0.010

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.333

Proposed MRL estimate

- Highest residue 0.010

- Mean + 4 SD 0.010

- CF x 3 Mean 0.010

Unrounded MRL 0.010

Rounded MRL 0.01

High uncertainty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 6

Pecan

Subsurface side-dress, SC, 1X0.41 lb ai/acre, PHI 57 days

One trial, cyantraniliprole and all metabolites each ND (<0.01 ppm).

Oilseed (group 20)

The tolerance estimate for the oilseed group 20 is 1.5 ppm, based on cotton field trial data. The 

OECD procedure calculated tolerances are 0.5 ppm for sunflower seed, 0.8 ppm for canola seed, 

and 1.5 ppm for cottonseed. The high and low values differ by a factor of <5X. The tolerance 

estimate for cotton gin byproducts is 10 ppm.

Cotton

Cyantraniliprole

Cottonseed

NAFTA
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 164 of 193

OD, 3X0.13, 7 d PHI

Total number of data (n) 13

Percentage of censored data 0%

Number of non-censored data 13

Lowest residue 0.012

Highest residue 0.990

Median residue 0.160

Mean 0.212

Standard deviation (SD) 0.249

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.990

- Mean + 4 SD 1.209

- CF x 3 Mean 0.635

Unrounded MRL 1.209

Rounded MRL 1.5

Residues (mg/kg) n

0.012 1

0.025 1

0.035 1

0.12 2

0.14 1

0.16 1

0.18 1

0.2 1

0.22 1

0.26 1

0.29 1

0.99 1

1 X 0.17 lb ai/A in-furrow with SC + 2 X 0.13 lb ai/A foliar

One study, 0.15 ppm

Insufficient data for estimation of tolerance.

Cyantraniliprole

Cotton gin byprod

NAFTA

OD, 3X0.13, 7 d PHI

Total number of data (n) 4
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 165 of 193

Percentage of censored data 0%

Number of non-censored data 4

Lowest residue 2.600

Highest residue 5.000

Median residue 3.100

Mean 3.450

Standard deviation (SD) 1.109

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 5.000

- Mean + 4 SD 7.886

- CF x 3 Mean 10.350

Unrounded MRL 10.350

Rounded MRL 10

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

2.6 1

2.7 1

3.5 1

5 1

Canola

Cyantraniliprole

Canola

NAFTA

OD, 3X0.13, 7 d PHI

Total number of data (n) 16

Percentage of censored data 0%

Number of non-censored data 16

Lowest residue 0.017

Highest residue 0.610

Median residue 0.102

Mean 0.157

Standard deviation (SD) 0.156

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.610

- Mean + 4 SD 0.779

- CF x 3 Mean 0.470

Unrounded MRL 0.779
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Page 166 of 193

Rounded MRL 0.8

cyantraniliprole

Canola

NAFTA

seed + 3 foliar

Total number of data (n) 4

Percentage of censored data 0%

Number of non-censored data 4

Lowest residue 0.015

Highest residue 0.210

Median residue 0.039

Mean 0.076

Standard deviation (SD) 0.090

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.210

- Mean + 4 SD 0.438

- CF x 3 Mean 0.227

Unrounded MRL 0.438

Rounded MRL 0.5

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.015 1

0.031 1

0.047 1

0.21 1

Sunflower

Cyantraniliprole

Sunflower seed

NAFTA

OD, 3X0.13, 7 d PHI

Total number of data (n) 8

Percentage of censored data 0%

Number of non-censored data 8
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Page 167 of 193

Lowest residue 0.028

Highest residue 0.320

Median residue 0.066

Mean 0.100

Standard deviation (SD) 0.095

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.320

- Mean + 4 SD 0.480

- CF x 3 Mean 0.301

Unrounded MRL 0.480

Rounded MRL 0.5

Residues (mg/kg) n

0.028 1

0.039 1

0.059 1

0.064 1

0.067 1

0.085 1

0.14 1

0.32 1
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 168 of 193

Tolerances for Raw Agricultural Commodities from Rotational Crop Field Trials

Root and Tuber Vegetables, Subgroup 1A

Rotational field trials with a 30 day PBI were conducted for turnip (n = 2), sugar beet (n = 14), 

carrot (n = 10), garden beet (n = 1), and radish (n =6). Residues were <0.01 for sugar beet root, 

garden beet root, and turnip root (n=2). Quantifiable residues were encountered with carrot and 

radish root. The tolerance estimate is 0.02 ppm, based on radish root. The value of 0.03 ppm 

petitioned by the registrant is too high, based on the data submitted.

Cyantraniliprole

Carrot (rotational)

NAFTA

1X seasonal

Total number of data (n) 10

Percentage of censored data 90%

Number of non-censored data 1

Lowest residue 0.010

Highest residue 0.011

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.400

Proposed MRL estimate

- Highest residue 0.011

- Mean + 4 SD 0.011

- CF x 3 Mean 0.012

Unrounded MRL 0.012

Rounded MRL 0.015

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 9

0.011 1

Cyantraniliprole

Radish root (rotational)

NAFTA

1X seasonal

Total number of data (n) 6
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 169 of 193

Percentage of censored data 83%

Number of non-censored data 1

Lowest residue 0.010

Highest residue 0.014

Median residue 0.010

Mean 0.011

Standard deviation (SD) 0.002

Correction factor for censoring (CF) 0.444

Proposed MRL estimate

- Highest residue 0.014

- Mean + 4 SD 0.017

- CF x 3 Mean 0.014

Unrounded MRL 0.017

Rounded MRL 0.02

High uncertainty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 5

0.014 1

Leaves of root and tuber vegetables (human food or animal feed) (crop group 2)

Trials were conducted for turnip tops (n = 6), sugar beet tops (n = 13), garden beet top (n = 1), 

and radish tops (n = 3). No quantifiable residues were found with garden beet tops and sugar 

beet tops. The OECD calculator provides an estimate of the tolerance of 0.04 ppm for leaves of 

root and tuber vegetables (crop group 2), based on radish tops. This agrees with the estimate of 

0.04 ppm from the registrant.

Cyantraniliprole

Turnip tops (rotational)

NAFTA

1X seasonal

Total number of data (n) 6

Percentage of censored data 83%

Number of non-censored data 1

Lowest residue 0.010

Highest residue 0.020

Median residue 0.010

Mean 0.012

Standard deviation (SD) 0.004

Correction factor for censoring (CF) 0.444

Proposed MRL estimate
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Page 170 of 193

- Highest residue 0.020

- Mean + 4 SD 0.028

- CF x 3 Mean 0.016

Unrounded MRL 0.028

Rounded MRL 0.03

High uncertainty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 5

0.02 1

Cyantraniliprole

Radish top

NAFTA

1X Seasonal

Total number of data (n) 3

Percentage of censored data 33%

Number of non-censored data 2

Lowest residue 0.010

Highest residue 0.021

Median residue 0.011

Mean 0.014

Standard deviation (SD) 0.006

Correction factor for censoring (CF) 0.778

Proposed MRL estimate

- Highest residue 0.021

- Mean + 4 SD 0.038

- CF x 3 Mean 0.033

Unrounded MRL 0.038

Rounded MRL 0.04

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

< 0.01 1

0.011 1

0.021 1
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Page 171 of 193

Foliage of legume vegetables, including soybean (crop group 7)

Eight trials were conducted for peas and beans. Seed (succulent and dry) cyantraniliprole 

concentrations were <0.01 ppm and detectable in only one instance. Finite residues were found 

on 3 forage samples and on 5 hay samples.

Sixteen rotational crop trials were conducted with soybean. No detectable residue was found on 

pod or seed (n = 13). The OECD calculator is used to estimate tolerances for soybean forage and 

soybean hay. 

The OECD estimates based on pea and bean data (0.3 ppm) are lower than those from soybean. 

The tolerance values are 0.2 ppm for soybean forage and 0.7 ppm for soybean hay. The 

registrant proposes 0.15 ppm for forage and 0.6 ppm for hay. Only one value may be used for 

this crop group, and the 0.6 ppm value is too low. The OECD estimate of the tolerance is 0.7 

ppm, based on soybean hay. 

Cyantraniliprole

Bean/Pea Forage (rotational)

NAFTA

1X seasonal

Total number of data (n) 8

Percentage of censored data 63%

Number of non-censored data 3

Lowest residue 0.010

Highest residue 0.031

Median residue 0.010

Mean 0.016

Standard deviation (SD) 0.009

Correction factor for censoring (CF) 0.583

Proposed MRL estimate

- Highest residue 0.031

- Mean + 4 SD 0.050

- CF x 3 Mean 0.028

Unrounded MRL 0.050

Rounded MRL 0.05

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 5

0.019 1
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 172 of 193

0.026 1

0.031 1

Cyantraniliprole

Bean/Pea Hay (rotational)

NAFTA

1X seasonal

Total number of data (n) 8

Percentage of censored data 38%

Number of non-censored data 5

Lowest residue 0.010

Highest residue 0.130

Median residue 0.022

Mean 0.054

Standard deviation (SD) 0.055

Correction factor for censoring (CF) 0.750

Proposed MRL estimate

- Highest residue 0.130

- Mean + 4 SD 0.274

- CF x 3 Mean 0.122

Unrounded MRL 0.274

Rounded MRL 0.3

Residues (mg/kg) n

< 0.01 3

0.017 1

0.027 1

0.11 1

0.12 1

0.13 1

Cyantaniliprole

Soybean forage

NAFTA

1X seasonal

Total number of data (n) 16

Percentage of censored data 69%

Number of non-censored data 5

Lowest residue 0.010

Highest residue 0.140

Median residue 0.010
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Page 173 of 193

Mean 0.026

Standard deviation (SD) 0.037

Correction factor for censoring (CF) 0.542

Proposed MRL estimate

- Highest residue 0.140

- Mean + 4 SD 0.173

- CF x 3 Mean 0.043

Unrounded MRL 0.173

Rounded MRL 0.2

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 11

0.016 1

0.024 1

0.038 1

0.091 1

0.14 1

Cyantraniliprole

Soybean hay

NAFTA

1X seasonal

Total number of data (n) 16

Percentage of censored data 19%

Number of non-censored data 13

Lowest residue 0.010

Highest residue 0.580

Median residue 0.017

Mean 0.066

Standard deviation (SD) 0.143

Correction factor for censoring (CF) 0.875

Proposed MRL estimate

- Highest residue 0.580

- Mean + 4 SD 0.639

- CF x 3 Mean 0.174

Unrounded MRL 0.639

Rounded MRL 0.7
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Page 174 of 193

Residues (mg/kg) n

< 0.01 3

0.011 2

0.013 2

0.015 1

0.018 1

0.024 1

0.029 1

0.033 1

0.048 1

0.055 1

0.18 1

0.58 1

Forage, fodder and straw of cereal grains (crop group 16)

There are 3 trials for oats, 2 for field corn, 2 for sweet corn, 3 for sorghum, 3 for wheat, and 1 for 

rice. The various forages, straws, and hays were estimated individually by crop type. The 

highest estimate of the tolerance is 0.5 ppm derived from 3 trials on wheat hay. The registrant 

proposed 0.06 ppm for cereal grain forage and 0.15 ppm for cereal grain hay and straw. Only 

one tolerance value is appropriate for a crop group, and the 0.15 ppm proposal is too low. The 

estimate is 0.5 ppm.
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Page 175 of 193

Compound

Crop

Region / Country

GAP

Total number of data (n)

Percentage of censored data

Number of non-censored data

Lowest residue

Highest residue

Median residue

Mean

Standard deviation (SD)

Correction factor for censoring (CF)

Proposed MRL estimate

- Highest residue

- Mean + 4 SD

- CF x 3 Mean

Unrounded MRL

Rounded MRL

Residues (mg/kg) Residues (mg/kg) Residues (mg/kg)

0.021 0.010 * 0.010 *

0.010 * 0.010 * 0.100

0.032 0.010 * 0.044

3

33%

Cyantraniliprole

wheat forage

Cyantraniliprole

oats forage

NAFTA rotational

1X seasonal

NAFTA roatational

1X seasonal

2

0.010

0.032

0.021

0.021

0.011

0.065

0.07

High uncertainty of MRL 

estimate due to small 

dataset.

0.778

0.032

0.065

0.049

0.010

0.010

0.010

0.010

0.000

0.333

0.010

0.010

0.010

0.010

0.01

High uncertainty of MRL 

estimate due to small 

dataset and high level 

of censoring.

2

0.010

3

33%

0.100

0.044

0.051

0.045

0.778

0.100

0.233

0.120

0.233

0.3

High uncertainty of MRL 

estimate due to small 

dataset.

0

Cyantraniliprole

sorghum forag

NAFTA rotational

1X seasonal

3

100%
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Page 176 of 193

Compound Cyantraniliprole Cyantraniliprole 

Crop Oats hay 

(rotational)

wheat hay 

(rotational)

Region / Country

GAP 1X seasonal 1X seasonal 

Total number of data (n) 3 3 

Percentage of censored data 0% 0% 

Number of non-censored data 3 3 

Lowest residue 0.019 0.022 

Highest residue 0.091 0.200 

Median residue 0.059 0.140 

Mean 0.056 0.121 

Standard deviation (SD) 0.036 0.091 

Correction factor for censoring 

(CF) 1.000 1.000 

Proposed MRL estimate

- Highest residue 0.091 0.200 

- Mean + 4 SD 0.201 0.483 

- CF x 3 Mean 0.169 0.362 

Unrounded MRL 0.201 0.483 

Rounded MRL 0.2 0.5 

High uncertainty of 

MRL estimate due to 

small dataset.

High uncertainty of 

MRL estimate due to 

small dataset.

Residues (mg/kg) Residues (mg/kg) 

0.059 0.022 

0.091 0.200 

0.019 0.140 

Cyantraniliprole

Hay All (Wheat + Straw)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 177 of 193

NAFTA Rotational

1X seasonal

Total number of data (n) 6

Percentage of censored data 0%

Number of non-censored data 6

Lowest residue 0.019

Highest residue 0.200

Median residue 0.075

Mean 0.089

Standard deviation (SD) 0.071

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.200

- Mean + 4 SD 0.373

- CF x 3 Mean 0.266

Unrounded MRL 0.373

Rounded MRL 0.4

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.019 1

0.022 1

0.059 1

0.091 1

0.14 1

0.2 1
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Page 178 of 193

Compound

Crop

Region / Country

GAP

Total number of data (n)

Percentage of censored data

Number of non-censored data

Lowest residue

Highest residue

Median residue

Mean

Standard deviation (SD)

Correction factor for censoring (CF)

Proposed MRL estimate

- Highest residue

- Mean + 4 SD

- CF x 3 Mean

Unrounded MRL

Rounded MRL

Residues (mg/kg) Residues (mg/kg) Residues (mg/kg) Residues (mg/kg) 

0.022 0.120 0.010 * 0.011 

0.049 0.010 * 0.010 * 0.079 

0.011 0.010 * 0.010 * 0.043 

0.010 * 

3

0%

Cyantraniliprole

Sorghum stover

Cyantraniliprole

Oats straw

1X seasonal 1X seasonal

3

0.011

0.049

0.022

0.027

0.020

0.106

0.15

High uncertainty of 

MRL estimate due to 

small dataset.

1.000

0.049

0.106

0.082

0.010

0.120

0.010

0.038

0.055

0.500

0.120

0.258

0.056

0.258

0.3

High uncertainty of 

MRL estimate due to 

small dataset and 

high level of 

censoring.

0

0.010

3

100%

0.010

0.010

0.010

0.000

0.333

0.010

0.010

0.010

0.010

0.01

High uncertainty of 

MRL estimate due to 

small dataset and 

high level of 

censoring.

Cyantraniliprole

Wheat straw

1X seasonal

3

0%

3

0.011

0.079

0.043

0.044

0.034

1.000

0.079

0.180

0.133

0.180

0.2

High uncertainty of 

MRL estimate due to 

small dataset.

1

Cyantraniliprole

Corn stover

1X seasaonal

4

75%
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Page 179 of 193

Grass forage, fodder, and hay (crop group 17)

Rotational crop trials were conducted for Bermuda grass (n = 2), bromegrass (n = 1), and 

bluegrass (n = 1). The crop results were combined (n = 4) for purposes of tolerance estimation. 

The tolerance estimate is 0.5 ppm, based on hay. The registrant proposed 0.06 ppm for forage 

and 0.15 ppm for hay. However, only one tolerance value may be established for a group, and 

the 0.15 ppm value is too low.

Cyantraniliprole

Grass forage

NAFTA

1X seasonal

Total number of data (n) 4

Percentage of censored data 25%

Number of non-censored data 3

Lowest residue 0.010

Highest residue 0.089

Median residue 0.011

Mean 0.030

Standard deviation (SD) 0.039

Correction factor for censoring (CF) 0.833

Proposed MRL estimate

- Highest residue 0.089

- Mean + 4 SD 0.187

- CF x 3 Mean 0.076

Unrounded MRL 0.187

Rounded MRL 0.2

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

< 0.01 1

0.011 2

0.089 1

Cyantraniliprole

Grass hay (rotat)

NAFTA

1X seasonal

Total number of data (n) 4

Percentage of censored data 0%

Number of non-censored data 4

Lowest residue 0.011
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Page 180 of 193

Highest residue 0.220

Median residue 0.024

Mean 0.070

Standard deviation (SD) 0.100

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.220

- Mean + 4 SD 0.471

- CF x 3 Mean 0.209

Unrounded MRL 0.471

Rounded MRL 0.5

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.011 1

0.023 1

0.025 1

0.22 1

Nongrass animal feeds (forage, fodder, straw, and hay) (crop group 18)

Rotational field trials were conducted for alafafa (n =12) and clover (n = 11). The tolerance 

estimate is 0.2 ppm based on alfalfa hay. The registrant proposed 0.06 ppm for forage and 0.15 

ppm for hay. However, only one tolerance value may be set for a group, and the 0.15 ppm value 

is too low.

Cyantraniliprole

alfalfa forage

NAFTA

1X seasonal

Total number of data (n) 12

Percentage of censored data 83%

Number of non-censored data 2

Lowest residue 0.010

Highest residue 0.053

Median residue 0.010

Mean 0.016

Standard deviation (SD) 0.014

Correction factor for censoring (CF) 0.444

Proposed MRL estimate

- Highest residue 0.053

- Mean + 4 SD 0.073
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Page 181 of 193

- CF x 3 Mean 0.021

Unrounded MRL 0.073

Rounded MRL 0.08

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 10

0.038 1

0.053 1

Cyantraniliprole

Clover forage

NAFTA

1X seasonal

Total number of data (n) 11

Percentage of censored data 91%

Number of non-censored data 1

Lowest residue 0.010

Highest residue 0.023

Median residue 0.010

Mean 0.011

Standard deviation (SD) 0.004

Correction factor for censoring (CF) 0.394

Proposed MRL estimate

- Highest residue 0.023

- Mean + 4 SD 0.027

- CF x 3 Mean 0.013

Unrounded MRL 0.027

Rounded MRL 0.03

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 10

0.023 1

Cyantraniliprole

Clover Hay
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 182 of 193

NAFTA

1X seasonal

Total number of data (n) 11

Percentage of censored data 73%

Number of non-censored data 3

Lowest residue 0.010

Highest residue 0.045

Median residue 0.010

Mean 0.017

Standard deviation (SD) 0.013

Correction factor for censoring (CF) 0.515

Proposed MRL estimate

- Highest residue 0.045

- Mean + 4 SD 0.067

- CF x 3 Mean 0.026

Unrounded MRL 0.067

Rounded MRL 0.07

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 8

0.029 1

0.033 1

0.045 1

Cyantraniliprole

Alfalfa Hay

NAFTA Rotational

1X Seasonal

Total number of data (n) 12

Percentage of censored data 67%

Number of non-censored data 4

Lowest residue 0.010

Highest residue 0.140

Median residue 0.010

Mean 0.029

Standard deviation (SD) 0.043

Correction factor for censoring (CF) 0.556

Proposed MRL estimate
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 183 of 193

- Highest residue 0.140

- Mean + 4 SD 0.201

- CF x 3 Mean 0.048

Unrounded MRL 0.201

Rounded MRL 0.2

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 8

0.01 1

0.021 1

0.097 1

0.14 1

Peanut hay

Thirteen rotational crop trials were conducted with peanut. The cyantraniliprole residue was 

non-detectable in all nutmeat samples. Cyantraniliprole was detected in 6 hay samples, but 

nonquantifiable (<0.01 ppm) in all 11 samples. An inadvertent tolerance estimate is not needed 

for peanut nutmeat as there is no reasonable expectation of residues of cyantraniliprole. Using 

the OECD calculator, the tolerance estimate for peanut hay is 0.01 ppm. The petitioner proposed 

0.03 ppm, but this value is too high.

Cyantraniliprole

Peanut hay

NAFTA

1X seasonal

Total number of data (n) 13

Percentage of censored data 100%

Number of non-censored data 0

Lowest residue 0.010

Highest residue 0.010

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.333

Proposed MRL estimate

- Highest residue 0.010

- Mean + 4 SD 0.010

- CF x 3 Mean 0.010

Unrounded MRL 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 184 of 193

Rounded MRL 0.01

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 13

Strawberry

Nine rotational crop trials were conducted with strawberry with a 30 day PBI. Values ranged 

from non-detectable to a maximum of 0.004 ppm (<0.01 LOQ). The OECD calculator estimates 

a tolerance of 0.01 ppm for crop subgroup 13-07G (strawberry). This represents a situation in 

which no residues are anticipated. The petitioner did not request an inadvertent tolerance, and 

none is needed.

The intervals from planting to harvest of the strawberries were 3 – 5 months. Some commercial 

production does not begin major harvest until about one year after planting. However, in 

California (80% of US production), perennial strawberries are generally managed as an annual 

crop and are removed one year after planting, although 6 – 7% of the acreage is keep for a 

second year of harvesting. Most fields are annually replanted from nursery stock to maximize 

crop vigor, avoid pest build-up in the soil, and maintain fruit quality throughout the harvest 

period. (The California Strawberry Commission (CSC); The California Minor Crops Council 

(CMCC))

Cyantraniliprole

Strawberry

NAFTA Rotational

1X Seasonal

Total number of data (n) 9

Percentage of censored data 100%

Number of non-censored data 0

Lowest residue 0.010

Highest residue 0.010

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.333

Proposed MRL estimate

- Highest residue 0.010

- Mean + 4 SD 0.010

- CF x 3 Mean 0.010

Unrounded MRL 0.010
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 185 of 193

Rounded MRL 0.01

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 9

Tolerances for Processed Commodities

Cyantraniliprole concentrated relative to the RAC in the following commodities: dry tomato 

pomace (1.8X), orange oil (6.2X), apple pomace dry (2.6X), and dried plum prune (1.5X). 

Tomato pomace is not a livestock feed item in the US, and dry apple pomace is not a feed item in 

the US. Wet apple pomace is fed, but no concentration was observed in the wet matrix. 

Cyantraniliprole concentrated slightly in apple puree (1.1X), but tolerances are not established 

for this commodity.

The OECD statistical calculation procedure is not used for processed commodities. Rather, the 

HAFT from the relevant field trials is multiplied by the median processing factor to arrive at an 

estimate.

Citrus Oil

The median processing factor for citrus oil is 6.2X. Application of this factor to the HAFT 

(orange, grapefruit, lemon), 0.39 ppm for orange, yields 2.4 ppm, that is 6.2 X 0.39. The 

tolerance estimate for citrus oil is 2.4 ppm. 

Dried Plum (Prune)

The median processing factor for dried plum is 1.5. Application of this factor to the HAFT of 

plum, 0.28 ppm, yields 0.42 ppm, that is, 1.5 X 0.28. . As 0.42 ppm is less than the 

recommended tolerance for the plum subgroup 12-12C at 0.5 ppm, a dried plum tolerance is not 

needed.
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 186 of 193

APPENDIX III: Number and Distribution of Field Trials for Various Crops Tested

NAFTA

Growing

Regions

TUBEROUS AND CORM VEGETABLES – CROP SUBGROUP 1C

POTATO TUBERS

No. of Trials Submitted Trial Requirements

CAN USA

1 7 7 2

2 1 1

3 1

4

5 4 5 2

6

7 2

8

9 1 1

10 1 1

11 5 4

12

13

14 1 2

15-21

Total 21 16 12

BULB VEGETABLES – CROP GROUP 3

NAFTA 

Growing

Region

DRY BULB ONIONS GREEN ONIONS

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA

1 1 1

2 1

3

4

5 5 5 2 3

6 1 1 1 1

7

8 1 1

9

10 2 2 1 2

11 1

12

13-21

Total 10 5 6 5 3 3

CROP GROUP 4 – LEAFY VEGETABLES

NAFTA

Growing

Region

HEAD LETTUCE LEAF LETTUCE CELERY SPINACH

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA CAN USA

1 1 1* 1 1* 1 1

2 1* 1* 1 1

3 1 1 1 1 1 1

4 2 3

5 4 5 4 5 5 5 1 1 1

6

7

8

9 1 1

10 5 4 5 4 5 4 3 2

11

12 1 1 1

13-21
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Total 12 5 6 12 5 6 11 5 6 10 3 6

NAFTA

Growing

Region

HEAD AND STEM BRASSICA VEGETABLES LEAFY BRASSICA

CSG 5A CSG 5A CSG 5A CSG 5B

BROCCOLI CAULIFLOWER CABBAGE MUSTARD GREENS

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA CAN USA

1 1 1 1 1 2

2 1 1 2 1

3 1 1 1

4 1 1

5 4 4 4 1 4 5 1 1 2 1

6 1 1 1 1 1

7

8

9

10 4 4 4 3 2 1 2 1

11

12 1 1 1 1 1 1 1

13-21

Total 9 5 6 4 5 6 11 5 6 11 3 5

FRUITING VEGETABLES – CROP GROUP 8-09

NAFTA

Growing

Region

TOMATOES BELL PEPPERS NON-BELL PEPPERS

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA CAN USA

1 1 1

2 1 1 1 1

3 2 2 1 1

4

5 8 12 1 5 5 1 5 5

6 1 1 1 1

7

8

9

10 8 7 3 2 3 2

11

12

13-21

Total 20 12 12 11 5 6 9 5 3

CUCURBIT VEGETABLES – CROP GROUP 9

NAFTA

Growing

Region

CUCUMBER CANTALOUPES SUMMER SQUASH

No. of Trials

Submitted

Trial 

Requirements No. of Trials

Submitted

Trial 

Requirements No. of Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA

1 1 1

2 2 2 1 1 1 1

3 2 1 1 1

4

5 4 5 2 3 5 1 3 4 1

6 1 1 1 1

7

8

9

10 4 3 2 1

11

12 1 1

13-21

Total 10 5 6 9 5 6 9 5 5
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CITRUS FRUITS – CROP GROUP 10

NAFTA

Growing

Region

ORANGES GRAPEFRUITS LEMONS

No. of Trials

Submitted

Trial 

Requirements No. of Trials

Submitted

Trial 

Requirements No. of Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA

1

2

3 8 8 3 3 1

4

5

6 1 1 1 1

7

8

9

10 4 3 3 2 6 4

11

12

13-21

Total 13 12 7 6 6 5

POME FRUITS – CROP GROUP 11

NAFTA

Growing

Region

APPLES PEARS

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA

1 3 3 3 1 1

2 1 1

3

4

5 5 7 2 4 4

6-8

9 1 1

10 1 1 2 2

11 4 2 4 4 1 3

12-21

Total 15 12 12 11 5 6

STONE FRUITS - CROP GROUP 12-09

NAFTA

Growing

Region

PEACHES PLUMS CHERRIES

No. of 

Trials

Submitted

Trial 

Requirements No. of 

Trials

Submitted

Trial 

Requiremen

ts

No. of Trials

Submitted

Trial Requirements

CAN USA CA

N

US

A

CAN USA

SC1 TC2SC1 TC2SC1 TC2

1 1 1 1 1

2 4 3

3

4 1

5 2 4 1 2 2 1 3 1 4 2 4

6 1 1

7

8

9 1 1 1

10 3 3 5 4 1 2

11 1 1 1 1 1 3 2

12 1 1

13

14 1

15-21

Total 13 5 9 9 3 6 2 5 5 5 6 6
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BLUEBERRIES

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1 4 4 1

2 2* 3

3

4

5 3 3 3

6

7

8

9

10

11

12 1 1 1

13-21

Total 10 8 8

TREE NUTS – CROP GROUP 14

NAFTA

Growing

Region

ALMONDS PECANS

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA

1

2 3 2

3

4 1 1

5

6 1 1

7

8 1 1

9

10 6 5

11

12

13-21

Total 6 -- 5 6 -- 5

COTTON

NAFTA

Growing Regions

No. of Trials

Submitted

Trial Requirements

CAN USA

1

2 1 1

3

4 3 3

5

6 1 1

7

8 5 4

9

10 3 3

11

12

13-21

Total 13 -- 12
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Page 190 of 193

OILSEED – CROP GROUP 20

NAFTA

Growing

Region

CANOLA SUNFLOWERS

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA

1

2 1 1

3

4

5 2 1 2 2 3 2

6

7 1 2 1 4 3

8 1 1

9

10

11 2 2

12

13

14 11 9 2 2

15-21

Total 17 12 6 9 5 6
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 191 of 193

Rotational Crops

The number and distribution of crop field trial guidance is taken from OCSPP 860.1500 and is 

based on requirements for primary crops. Some latitude is appropriate for those commodities 

which are livestock feed items only.

NAFTA

Growing

Region

ROOT VEGETABLES – CROP SUBGROUP 1A1

CARROT RADISH SUGAR BEET

No. of Trials

Submitted

Trial 

Requirements

No. of Trials

Submitted

Trial 

Requirements

No. of Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA

1 1 1 1

2 2

3 1 1 2

4

5 3 1 1 1 6 5

6 1 1

7 4 1

8 1 1

9

10 3 3 1 1 1 1

11 1 1 2 1

12

13-21

Total 10 6 6 5 14 9

1 Trials were also provided for turnip root (n =2) and garden beet (n = 1).

LEAVES OF ROOT AND TUBER VEGETABLES - CROP GROUP 21

NAFTA

Growing

Region

TURNIP SUGAR BEET

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA

1

2 2 2

3

4 1

5 3 1 4 5

6 1 1

7 4 1

8 1 1

9

10 1 1

11 2 1

12

13

14 1

15-21

Total 6 5 13 9

1 Trials were also provided for radish top (n =3) and garden beet top (n = 1)
Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961

Page 192 of 193

FOLIAGE OF LEGUME VEGETABLES – CROP GROUP 7

NAFTA

Growing

Region

BEANS PEAS SOYBEAN

No. of Trials

Submitted

Trial 

Requirements No. of Trials

Submitted

Trial 

Requirements No. of Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA

1

2 1 1 2

3

4 2

5 1 2 9 11

6

7 3

8

9

10 1

11 2 2 2

12

13-21

Total 4 3 4 3 16 15

NAFTA

Growing

Region

FORAGE,FODDER AND STRAW OF CEREAL GRAINS – CROP GROUP 161

Corn (field) Oats Sorghum Wheat

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

No. of 

Trials

Submitted

Trial 

Requirements

CAN USA CAN USA CAN USA CAN USA

1 1 1

2 2 1 1 1 1

3

4 1 1

5 2 12 1 6 2 3 1 3

6 1 1 2 1

7 2 1 4

8 1 2 4

9

10 1 1

11 1 1 1

12

13-21

Total24 15 3 -

3

3 -

3

3 15

1 One trial was also provided for rice. Total provided = 14

2 Total = 42.

3

Forages/fodders/straws other than corn and wheat should total 12.

GRASS FORAGE, FODDER, AND HAY – CROP GROUP 17

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1

2

3

4

5 3

6

7

8

9

10 1

11

12

13-21
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Page 193 of 193

Total 4 9

NONGRASS ANIMAL FEEDS – CROP GROUP 18

NAFTA

Growing

Region

ALFALFA CLOVER

No. of Trials

Submitted

Trial Requirements No. of Trials

Submitted

Trial Requirements

CAN USA CAN USA

1 1 1 2 1

2 1

3

4 1

5 3 4 3 2

6 1 1

7 3 1 2 1

8 1

9 1

10 2 1 1 1

11 1 1 1

12

13

14 2 1

15-21

Total 12 9 11 9

PEANUT HAY

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1

2 9 5

3 1 1

4

5

6 3 2

7

8 1

9

10

11

12

13-21

Total 13 9

1

1

For a no residue situation. Otherwise, 12 trials.

LOW GROWING BERRY SUBGROUP 13-07G (STRAWBERRY)

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1 2 1

2

3 1 1

4

5 1 1

6

7

8

9

10 4 2

11

12 1 1

13-21

Total 9 6

1

1

Number required for strawberry as part of crop group or more a no residue situation. Otherwise, 8.