Data Requirement 

EPA OPPTS 870.3700 

OECD 414 

HLS STUDY NO. 09-4348 

SPONSOR STUDY NO. MA-252E-93-23 

EMBRYO-FETAL TOXICITY STUDY IN RABBITS 

VIA WHOLE-BODY INHALATION EXPOSURE (GLP) 

Final Report 

Author: Gary M. Hoffman, BA, DABT 

Performed by: Huntingdon Life Sciences 

100 Mettlers Road 

East Millstone, New Jersey 08875-2360 

 Submitted to:

Attn: 

Date: 2 September 2010 

Page 1 of 298 

SANITIZED
SANITIZED
SANITIZED
SANITIZED
Huntingdon Life Sciences 09-4348 Page 5 

 MA-252E-93-23 Final Report

LIST OF ABBREVIATIONS 

Abbreviation Term 

F Female(s) 

GD or GES Gestation Day 

hr(s) hour(s) 

Lpm Liters per minute 

mg/L milligrams of test substance per Liter of air 

min(s) minute(s) 

NOAEL No-Observed-Adverse-Effect-Level 

NOEL No-Observed-Effect-Level 

np non-pregnant 

ppm parts per million 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 6 

 MA-252E-93-23 Final Report

Embryo-Fetal Toxicity Study in Rabbits 

via Whole-Body Inhalation Exposure (GLP) 

SUMMARY 

The objective of this study was to assess any gross maternal and/or embryo-fetal toxicity 

of in a non-rodent species, the rabbit, following whole-body inhalation 

exposure (every day from Gestation Day (GD) 6 to GD 28, inclusively), covering the 

period of major organogenesis (which extends from implantation to the day prior to 

expected delivery, approximately GD 6 to GD 28). 

Eighty-eight timed-mated female New Zealand White rabbits were distributed into 

4 groups (1 control and 3 treated groups) of 22 dams/group. These animals received 

whole-body inhalation exposures every day with clean air (air control) or with 

at exposure concentrations of 2500, 10000 or 15000 ppm from GD 6 to GD 28 

(GD 0 = day of mating confirmation). The exposure durations were 6 hours per day for 

7 days per week. 

All animals were euthanized and necropsied on GD 29. The following parameters were 

evaluated for all animals: viability, clinical observations, body weights and feed 

consumption. A macroscopic postmortem evaluation was performed on GD 29, during 

which corpora lutea and implantation sites were counted and uterine and placental 

weights were recorded. Fetuses were removed, weighed (live fetuses only), sexed and 

examined externally for defects, for soft tissue abnormalities and for skeletal 

abnormalities and ossification state. 

The target and mean analytical (IR) and nominal concentrations are summarized as follows: 

Group 

Target

Concentration 

(ppm)

Analytical

Concentration 

(ppm)

Nominal 

Concentration 

(ppm) 

1 0 0.0 0 

2 2500 2513 NA 

3 10000 9945 NA 

4 15000 14942 30154* 

*the combined nominal concentration for all 3 exposed groups 

There were no effects of exposures on survival, clinical signs, body weights, food 

consumption or maternal or fetal findings at sacrifice. Therefore, the no-observed-effect 

level (NOEL) for adult pregnant female rabbits for systemic and reproductive and 

developmental toxicity was 15,000 ppm of 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 7 

 MA-252E-93-23 Final Report

TABLE OF CONTENTS 

COVER PAGE .....................................................................................................................1

STATEMENT OF COMPLIANCE .....................................................................................2

SIGNATURE PAGE ...........................................................................................................3

QUALITY ASSURANCE STATEMENT ..........................................................................4

LIST OF ABBREVIATIONS ..............................................................................................5

SUMMARY .........................................................................................................................6

TABLE OF CONTENTS .....................................................................................................7

1. INTRODUCTION ................................................................................................10

2. MATERIALS AND METHODS

2.1. Study Management ....................................................................................10

2.2. Study Dates ................................................................................................10

2.3. Experimental Outline .................................................................................11

2.4. Justifications ..............................................................................................11

2.5. Test Substance ...........................................................................................13

2.6. Test Animals ..............................................................................................15

2.7. Animal Assignment ...................................................................................15

2.8. Animal Identification .................................................................................16

2.9. Veterinary Care ..........................................................................................16

2.10. Husbandry During Non-Exposure Periods ................................................16

2.11. Husbandry During Exposure Periods ........................................................18

2.12. Test Substance Administration and Chamber Operation ...........................19

2.13. Experimental Evaluations ..........................................................................23

2.14. Terminal Observations ...............................................................................23

2.15. Statistical Analysis .....................................................................................25

2.16. Major Computer Systems ..........................................................................28

2.17. Data Storage ...............................................................................................28

2.18. Regulatory References ...............................................................................29

2.19. Protocol Deviation .....................................................................................29

3. RESULTS AND DISCUSSION

3.1. Chamber Monitoring ..................................................................................30

3.2. Maternal Data ............................................................................................31

3.3. Fetal Data ...................................................................................................32

4. CONCLUSION ....................................................................................................33

SANITIZED
Huntingdon Life Sciences 09-4348 Page 8 

 MA-252E-93-23 Final Report

TABLE OF CONTENTS 

REFERENCES ..................................................................................................................34

CALCULATIONS .............................................................................................................35

GENERAL PREFACE ......................................................................................................36

FIGURES 

1. Diagram of Generation System and Exposure Chamber - Group 1 .............................37

Diagram of Generation System and Exposure Chamber - Groups 2-4 ........................38

2. Mean Body Weights ....................................................................................................39

3. Mean Food Consumption Values.................................................................................40

TABLES

1. Summary of Survival ...................................................................................................41

2. Summary of In-Chamber Observations .......................................................................42

3. Summary of Daily Observations ..................................................................................44

4. Summary of Physical Examination Findings ...............................................................48

5. Mean Body Weights ....................................................................................................53

6. Mean Body Weight Changes .......................................................................................54

7. Mean Food Consumption Values.................................................................................55

8. Incidence Summary Report for Gross Necropsy Observations ...................................56

9. Summary of Pregnancy ................................................................................................58

10. Summary of Cesarean Section Data ............................................................................59

11. Summary of Gravid Uterine Adjusted Body Weights .................................................60

12. Mean Litter Weights ....................................................................................................61

13. Summary of Fetal Observations ...................................................................................62

External ........................................................................................................................63

Visceral ........................................................................................................................64

Skeletal .........................................................................................................................67

Ossification Parameters ...............................................................................................71

APPENDICES

A. Chamber Monitoring Results .......................................................................................74

B. Individual Animal Termination History ......................................................................82

C. Individual Daily Observations .....................................................................................86

D. Individual Physical Examination Findings ..................................................................93

E. Individual Body Weights ...........................................................................................114

F. Individual Body Weight Changes ..............................................................................122

G. Individual Food Consumption Values .......................................................................130

H. Raw Data Listing of Gross Observations with Modifiers ..........................................138

SANITIZED
Huntingdon Life Sciences 09-4348 Page 9 

 MA-252E-93-23 Final Report

TABLE OF CONTENTS 

I. Individual Cesarean Section Data ..............................................................................144

J. Individual Gravid Uterine Adjusted Body Weights ..................................................152

K. Individual Litter Weight Data ....................................................................................160

Individual Litter Fetal and Placental Weights ...........................................................161

Individual Fetal Weights ............................................................................................169

Individual Placental Weights .....................................................................................177

L. Individual Fetal Observations ....................................................................................185

M. Equipment List ...........................................................................................................284

N. Pre-study Chamber Distribution Samples ..................................................................286

O. Miran Calibration .....................................................................................................288

P. Certificates of Analysis ..............................................................................................291

Q. Scientists and Supervising Personnel ........................................................................298

SANITIZED
Huntingdon Life Sciences 09-4348 Page 10 

 MA-252E-93-23 Final Report

1. INTRODUCTION 

The purpose of this study was to assess gross maternal and/or embryo-fetal 

toxicity of in a non-rodent species, the rabbit, following whole 

body inhalation exposure (every day from Gestation Day (GD) 6 to GD 28, 

inclusively), covering the period of major organogenesis (which extends from 

implantation to the day prior to expected delivery, approximately GD 6 to 

GD 28). 

2. MATERIALS AND METHODS 

2.1. STUDY MANAGEMENT 

2.1.1. SPONSOR 

2.1.2. SPONSOR REPRESENTATIVE 

2.1.3. TESTING FACILITY 

Huntingdon Life Sciences 

100 Mettlers Road 

East Millstone, New Jersey 08875-2360 

2.1.4. STUDY DIRECTOR 

Gary M. Hoffman, BA, DABT 

2.2. STUDY DATES 

2.2.1. STUDY INITIATION 

17 February 2010 (Date Study Director signed the Protocol) 

2.2.2. ANIMAL RECEIPT 

10 and 12 March 2010 (Experimental Start Date) 

2.2.3. EXPOSURE INITIATION 

14, 15, 16 or 17 March 2010 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 11 

 MA-252E-93-23 Final Report

2.2.4. EXPOSURE TERMINATION 

5, 6, 7 or 8 April 2010 

2.2.5. TERMINAL SACRIFICE 

6, 7, 8 or 9 April 2010 

2.2.6. EXPERIMENTAL COMPLETION 

26 May 2010 

2.2.7. STUDY COMPLETION 

2 September 2010 (Date Study Director signed the Final Report) 

2.3. EXPERIMENTAL OUTLINE 

Four (4) experimental groups (1 control and 3 treated groups) received 

whole-body inhalation exposures every day with clean air or with 

 at the exposure concentrations summarized below. The 

animals were exposed every day from Gestation Day (GD) 6 to GD 28 

(GD 0 = day of mating). Each group consisted of 22 time-mated females 

as outlined below: 

 Exposure Levelsa Test Animals (females) 

Group 

Group 

Designation (ppm) (mg/L) 

Total on 

Study Necropsy

1 Air Control 0 0 22 22 

2 Low 2500 13 22 22 

3 Mid 10000 53 22 22 

4 High 15000 80 22 22 a

Exposures were 6 hours per day for 7 consecutive days per week. Exposure levels are 

expressed as ppm and equivalent mg/L of test substance; results are reported in ppm. 

The exposures were conducted via whole-body exposure. 

2.4. JUSTIFICATIONS 

2.4.1. ROUTE, DURATION AND FREQUENCY OF 

ADMINISTRATION 

The inhalation route is one of the potential routes of human 

exposure to this test article. The duration and frequency of 

exposures were as recommended in the relevant test guideline, 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 12 

 MA-252E-93-23 Final Report

ensuring adequate exposure of post-implantation embryo-fetal 

development.

2.4.2. EXPOSURE LEVEL SELECTION 

A pilot 4-week inhalation study was performed with male and 

female CD rats exposed to target levels of 0, 2000, 4500, 7500 and 

10000 ppm of Effects were limited to mild 

reversible alterations in some hematology parameters at 

10000 ppm and to a lesser extent at 7500 ppm. Based on the above 

information, a high exposure level of 15000 ppm was selected for a 

range-find rabbit inhalation study (Huntingdon Life Sciences 

Study No. 09-4347) to provide slight maternal toxicity while lower 

levels of 10000 and 2500 ppm were selected to provide a range of 

possible effects including a NOEL or NOAEL. The range-find 

rabbit inhalation study showed no effect at all exposure levels and 

therefore the same exposure levels were selected for this study. 

2.4.3. TEST ANIMAL SELECTION 

The rabbit is accepted by Regulatory Authorities as a surrogate for 

humans in the detection of effects on embryo-fetal development. It 

is the preferred non-rodent species for developmental toxicity 

testing, for practical reasons and in view of the large amount of 

accumulated background information in the species. Also a rangefind rabbit inhalation study (Huntingdon Life Sciences Study 

No. 09-4347) was conducted with this test substance in preparation 

for this definitive study. 

2.4.4. NUMBER OF ANIMALS 

The number of animals in this study (22 time-mated 

females/group) was considered the minimum necessary to allow 

for meaningful interpretation of the outcome and was based on the 

following key concepts: 

• The OECD and EPA guidelines indicate that no less than 

16 pregnancies/group be evaluated. Some of the endpoints of 

principal interest (e.g., embryonic death, malformation) are 

low-frequency events. 

• A reduction in litters available for evaluations is possible due 

to the following events: 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 13 

 MA-252E-93-23 Final Report

Low pregnancy rate. The normally expected pregnancy rate in 

this strain from our current vendors is approximately 

85 to 95%. 

Unexpected deaths and/or abortions. 

A number of 22 time-mated females provides a sufficient 

number of pregnancies for evaluation in each group (as it 

allows for ≥16 pregnancies/group, even if half the potentially 

not pregnant females are assigned to a particular group). 

Further to this, 22 rabbits per group reduces the risk of having 

to repeat the study due to an insufficient number of litters for 

evaluation. 

At least three exposure levels are required by regulatory agencies 

to describe an exposure relationship in any effects observed, in 

comparison with an air control group. 

2.5. TEST SUBSTANCE 

2.5.1. TEST SUBSTANCE CATEGORY 

Industrial chemical used as a possible refrigerant and solvent. 

2.5.2. MANUFACTURER/SUPPLIER 

2.5.3. LOT NUMBERS 

41024-38 

KP-4 

BZ056A-1 

BZ056A-2 

BZ056A-3 

BZ056A-4 

BZ056B-1 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 14 

 MA-252E-93-23 Final Report

2.5.4. PURITY 

Approximately 100% as per Certificates of Analysis 

2.5.5. DESCRIPTION 

Liquid in cylinder 

2.5.6. DATES RECEIVED 

41024-38: 18 May 2009 

KP-4: 17 June 2009 

BZ056A-1: 2 March 2010 

BZ056A-2: 2 March 2010 

BZ056A-3: 2 March 2010 

BZ056A-4: 2 March 2010 

BZ056B-1: 17 March 2010 

2.5.7. EXPIRATION DATES 

41024-38: 13 May 2010 

KP-4: 15 June 2010 

BZ056A-1: 3 March 2011 

BZ056A-2: 3 March 2011 

BZ056A-3: 3 March 2011 

BZ056A-4: 3 March 2011 

BZ056B-1: 3 March 2011 

2.5.8. ANALYSIS 

Documentation of the identity, strength, purity, composition, 

stability, and method of synthesis, fabrication, and/or derivation of 

the test substance and the maintenance of these records were the 

responsibility of the Sponsor. Certificates of Analysis were 

provided by the Sponsor and are included in Appendix P. 

2.5.9. STORAGE 

Room temperature, protected from light 

2.5.10. ARCHIVAL SAMPLE 

A sample from each lot of test substance is stored in the Archives 

of the Testing Facility. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 15 

 MA-252E-93-23 Final Report

2.5.11. DISPOSITION 

The unused portion of the test substance and any empty test 

substance containers were returned to the Sponsor on 12 July 2010. 

2.6. TEST ANIMALS 

2.6.1. SPECIES 

Albino Rabbits 

New Zealand White 

2.6.2. SUPPLIER 

Covance Research Products 

Denver, Pennsylvania 

2.6.3. NUMBER OF ANIMALS 

Received/Placed on test: 

88 timed-mated females 

2.6.4. AGE AT RECEIPT 

Approximately 5 to 6 months at receipt on GD 1 or 2, where GD 0 

is the day of mating. 

2.6.5. AGE AT INITIATION OF EXPOSURE 

Approximately 5 to 6 months old. 

2.6.6. WEIGHT AT INITIATION OF EXPOSURE (KILOGRAMS) 

Mean Range 

3.4 2.9 to 4.1 

2.6.7. STABILIZATION PERIOD 

The stabilization period was restricted to approximately 4 to 5 days 

prior to commencement of exposures, as dictated by the mating 

and delivery schedule for the animals. 

2.7. ANIMAL ASSIGNMENT 

The exact number of animals required for the study were purchased and 

stabilized. Animals were randomly assigned to control or treated groups 

upon arrival (4 groups of 22 animals/group). Prior to exposures, the body 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 16 

 MA-252E-93-23 Final Report

weights obtained on GD 4 were reviewed to ensure minimization of intergroup variability based on those body weights. 

2.8. ANIMAL IDENTIFICATION 

Each animal was identified with an ear-tag bearing its assigned animal 

number. The assigned animal number plus the study number comprised 

the unique animal number for each animal. In addition, each cage was 

provided with a cage card which was color-coded for exposure level 

identification and contained study number and animal number 

information. 

2.9. VETERINARY CARE 

Animals were monitored by the technical staff for any conditions requiring 

possible veterinary care and treated as necessary. Miscellaneous, non-test 

article-related veterinarian evaluations and treatments (as necessary) for 

individual animals were reviewed by the study director and are 

documented in the study file. 

2.10. HUSBANDRY DURING NON-EXPOSURE PERIODS 

2.10.1. FACILITIES MANAGEMENT/ANIMAL HUSBANDRY 

Currently acceptable practices of good animal husbandry were 

followed e.g., Guide for the Care and Use of Laboratory Animals; 

National Academy Press, 1996. Huntingdon Life Sciences, East 

Millstone, New Jersey is fully accredited by the Association for 

Assessment and Accreditation of Laboratory Animal Care 

International (AAALAC). 

2.10.2. HOUSING 

All animals were housed individually in stainless steel suspended 

cages with grid bottoms. A stainless steel food hopper was 

attached to each cage. 

2.10.3. FEED 

Global High Fiber Diet #2031C (Harlan Teklad, Madison, 

Wisconsin) was available in measured amounts, up to 200 grams 

per day. Fresh feed was presented daily. Feed was presented at 

approximately the same time each day. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 17 

 MA-252E-93-23 Final Report

2.10.4. FEED ANALYSIS 

Analysis of each feed lot used during this study was performed by 

the manufacturer. Results were provided to the Testing Facility 

and are maintained on file at the Testing Facility. There were no 

known contaminants in the feed that were expected to interfere 

with the results of this study. 

2.10.5. WATER 

Water (Elizabethtown Water Company, Westfield, New Jersey) 

was available without restriction via an automated watering 

system. 

2.10.6. WATER ANALYSIS 

Water analyses are conducted by Elizabethtown Water Company, 

Westfield, New Jersey (Raritan-Millstone Plant) to ensure that 

water meets standards specified under the EPA Federal Safe 

Drinking Water Act Regulations (40 CFR Part 141). In addition, 

water samples are collected biannually from representative rooms 

in the Testing Facility; chemical and microbiological water 

analyses are conducted on these samples by a subcontract 

laboratory. Results of all water analyses are maintained on file at 

the Testing Facility. There were no known contaminants in the 

water which were expected to interfere with the results of this 

study. 

2.10.7. ENVIRONMENTAL CONDITIONS 

Light/Dark Cycle 

A twelve-hour light/dark cycle controlled via an automatic timer 

was provided. 

Temperature 

Temperature was monitored in accordance with Testing Facility 

SOPs and maintained within the specified range to the maximum 

extent possible. 

Desired Range: 16 to 22 °C 

Daily Average Range: 18 to 20 °C 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 18 

 MA-252E-93-23 Final Report

Relative Humidity 

Relative humidity was monitored in accordance with Testing 

Facility SOPs and maintained within the specified range to the 

maximum extent possible. 

Desired Range: 30 to 70% 

Daily Average Range: 39 to 68% 

Air Changes 

The number of air changes in the animal rooms is maintained at 10 

to 15 air changes per hour and monitored at least twice each year 

in accordance with Testing Facility SOPs. 

2.10.8. ENRICHMENT 

Animals were provided with enrichment devices (toys and/or feed 

treats) in accordance with Testing Facility SOPs. 

2.11. HUSBANDRY DURING EXPOSURE PERIODS 

Animals were individually housed in suspended, stainless steel wire mesh 

cages. 

2.11.1. FEED 

None was provided during exposure. 

2.11.2. WATER 

None was provided during exposure. 

2.11.3. ENVIRONMENTAL CONDITIONS 

Chamber temperature and relative humidity were recorded every 

half-hour during exposure and maintained, to the maximum extent 

possible, within the ranges presented below. Excursions outside 

the specified range did not affect the integrity of the study. 

Temperature 

Desired: 16 to 22 °C 

Actual: 17 to 23 °C 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 19 

 MA-252E-93-23 Final Report

Relative Humidity 

Desired: 40 to 60% 

Actual: 47 to 66% 

2.12. TEST SUBSTANCE ADMINISTRATION AND 

CHAMBER OPERATION 

2.12.1. ROUTE OF ADMINISTRATION 

Inhalation as a vapor by whole-body exposure. 

2.12.2. FREQUENCY AND DURATION OF ADMINISTRATION 

Animals were exposed for 6 hours per day once every day at 

approximately the same time each day, on GD 6 to 28 inclusive, 

where GD 0 was the day of mating. 

2.12.3. PRE-STUDY TRIALS 

The test substance was administered as a vapor in the breathing air 

of the animals. The test atmosphere was generated by an 

appropriate procedure determined during pre-study trials. The 

trials were performed to evaluate the optimal set of conditions and 

equipment to generate a stable atmosphere at the target exposure 

levels and maintain uniform conditions throughout the exposure 

chambers. 

2.12.4. EXPOSURE PROCEDURE 

Group 1 

Houseline nitrogen was delivered from a regulator with a 

backpressure gauge via 1⁄4” tubing to a flowmeter regulated by a 

metering valve. This nitrogen flow (~ 30 Lpm) was then directed 

into the chamber turret of the 1.5 m3 glass and stainless steel 

exposure chamber where it was mixed with room air as it was 

drawn into the chamber. 

Groups 2, 3 and 4 

Houseline nitrogen was delivered from a regulator with a 

backpressure gauge through a stainless steel fitting to create three 

flow systems: the test substance pressurization flow, the purge 

flow and the volatilization flow. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 20 

 MA-252E-93-23 Final Report

The nitrogen for the test substance pressurization flow was 

delivered from a regulator with a backpressure gauge via 1⁄4” 

tubing through a toggle valve attached to a backpressure gauge into 

the vapor inlet valve of the test substance cylinder. The houseline 

nitrogen regulator valve was used to adjust and maintain the 

pressure within the cylinder. From the pressurized cylinder, the 

test substance flowed from the liquid outlet valve through another 

toggle valve then through a filter to prevent equipment 

contamination. From the filter, the test substance was directed into 

a stainless steel “T” piece where one side of the “T” was directed 

towards the Group 2 exposure system, while the other side of the 

“T” piece was directed towards the Groups 3 and 4 systems where 

a second stainless steel “T” split the test substance flow and 

directed it to the Group 3 and Group 4 exposure systems. 

The exposure system set-up and delivery was the same for all test 

groups. From the “T” pieces after the filter, the test substance 

flowed to a liquid flowmeter with a built-in regulator via 1⁄8” 

tubing. The outlet of the flowmeter was regulated by a metering 

valve. From this metering valve, the test substance flowed via 1⁄8” 

tubing onto the glass helix of a counter current volatilization 

chamber. This glass helix was heated by a nichrome wire which 

was controlled by an autotransformer and was inserted in the 

center of the glass tube that supported the helix. Due to the flow 

rate necessary to reach the required chamber concentrations, single 

flowmeter/glass helix and counter current systems were used for 

Groups 2 and 3, while two identical flowmeter/glass helix and 

counter current systems were used for Group 4. 

The nitrogen for the purge flow system was directed, via 1⁄4” tubing 

to single flowmeters regulated by metering valves for Groups 2 

and 3 or to two flowmeters regulated by metering valves for 

Group 4. The purge nitrogen was delivered via 1⁄8” tubing to the 

bottom of the tube containing the nichrome wire. This nitrogen 

flow continuously purged the area surrounding the nichrome wire 

within the tube, thereby, protecting the wire from oxidation. 

The nitrogen for the volatilization system was directed via 1⁄4” 

tubing to single flowmeters regulated by metering valves for 

Groups 2 and 3 or to two flowmeters regulated by metering valves 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 21 

 MA-252E-93-23 Final Report

for Group 4. From the flowmeter, the volatilization nitrogen 

(Groups 2 and 3 ~ 30 Lpm; Group 4 ~ 15 Lpm) flowed via 1⁄4” 

tubing to a ball and socket joint at the bottom of the volatilization 

chamber. This nitrogen flowed up through the volatilization 

chamber passing over the coil and volatilizing the test substance. 

This test substance laden nitrogen exited the top of the 

volatilization chamber via 1⁄4” tubing, to the turret of the 1.5 m3

glass and stainless steel exposure chamber. As the test substance 

laden nitrogen was drawn into each of the chambers, it was mixed 

with room air. 

See Figure 1 and Appendix M for equipment details. 

2.12.5. CHAMBER OPERATION 

The whole-body exposure chambers each had a volume of 

approximately 1500 Liters (1.5 m3). Each chamber was operated 

at a minimum flow rate of 300 Liters per minute. The final airflow 

was set to provide at least one air change (calculated by dividing 

the chamber volume by the airflow rate) in 5.0 minutes (12 air 

changes/hour) and a T99 equilibrium time (calculated by 

multiplying the air change by the exponential factor 4.6) of at most 

23 minutes: 

Group Airflow Rate Air Change T99

(Lpm) (min) (min)

1 312 4.8 22

2 308 4.9 22

3 302 5.0 23

4 305 4.9 23

This chamber size and airflow rates were considered adequate to 

maintain the oxygen level at least 19% and the animal loading 

factor below 5%. At the end of each exposure, all animals 

generally remained in the chamber for a minimum of 23 minutes. 

During this time, each chamber was operated at approximately the 

same flow rate using clean air only. The chambers were exhausted 

through the in house filtering system, which consisted of a coarse 

filter, a HEPA filter and activated charcoal. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 22 

 MA-252E-93-23 Final Report

See Figure 1 and Appendix M for equipment details. 

2.12.6. EXPOSURE CONCENTRATION DETERMINATION 

The nominal concentration was determined by weighing the 

generation apparatus containing the test substance before and after 

the exposure and calculating as follows: 

Conc (ppm) = amount consumed (g) x 1000 (mg/g) x 1000 (μg/mg) x 22.4 (μL/μmole) x 292 K

exposure duration (min) x airflow (Lpm) x MW (μg/μmole) x 273 K 

MW = 130.5 μg/μmole 

The nominal concentration was calculated for all three test 

substance-treated groups combined. 

The flow of air through the chamber was monitored using 

appropriate calibrated equipment. The test substance consumed 

(weight difference of the cylinder) during the exposure (kg) was 

divided by the total volume of air (m3) passing through the 

chamber to calculate the nominal concentration (ppm). 

During each exposure, measurements of airborne concentrations 

were performed in the animals’ breathing zone at least 4 times using 

an appropriate sampling procedure and infrared (IR) 

spectrophotometric analytical procedure. 

See Appendix M for equipment details. 

2.12.7. PARTICLE SIZE DISTRIBUTION ANALYSIS 

During each week of exposure, particle size determinations were 

performed using a TSI Aerodynamic Particle Sizer to characterize 

the aerodynamic particle size distribution of any aerosol present. 

The samples were drawn for 20 seconds at a flow rate of 5.0 Lpm. 

The mass median aerodynamic diameter, geometric standard 

deviation and total mass concentration were calculated based on 

the amount of particles collected. 

See Appendix M for equipment details. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 23 

 MA-252E-93-23 Final Report

2.13. EXPERIMENTAL EVALUATIONS 

2.13.1. VIABILITY CHECKS 

Animals were observed in their cages twice daily (once in the 

morning and once in the afternoon) for mortality, morbidity and 

signs of severe toxicity. 

2.13.2. PHYSICAL OBSERVATIONS 

Exposure Observations 

All visible animals were observed as a group at least once during 

each exposure. 

Detailed Physical Examinations 

Animals were removed from their cages and examined daily from 

receipt to terminal euthanasia. Examinations included 

observations of general condition, skin and fur, eyes, nose, oral 

cavity, abdomen and external genitalia as well as evaluations of 

respiration. Examinations were performed after exposures during 

the exposures period. 

2.13.3. BODY WEIGHT 

Body weights were recorded for all animals on the day of arrival 

(for health monitoring; weights not included in this report) and on 

presumed GD 4, 6, 9, 12, 15, 18, 20, 23, 26 and 29. 

2.13.4. FOOD CONSUMPTION 

Feed was available without restriction 7 days/week. Animals were 

presented with a known quantity of feed each day. The weight of 

the feed remaining on the following day was subtracted from the 

quantity fed original to obtain the grams consumed. Food 

consumption was measured (weighed) daily from GD 4 through 

GD 29. 

2.14. TERMINAL OBSERVATIONS 

2.14.1. NECROPSY INFORMATION 

Necropsy was performed on 22 animals/group after 23 days of 

exposure. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 24 

 MA-252E-93-23 Final Report

Method of Euthanasia 

Adult females: intravenous injection of sodium pentobarbital. 

Fetuses: intraperitoneal injection of sodium pentobarbital. 

2.14.2. TERMINAL EXAMINATIONS (SCHEDULED 

TERMINATIONS) 

A necropsy (macroscopic examination) comprised of an external 

examination followed by an internal examination (excluding the 

cranial cavity) was performed. Minor sporadic lesions common to 

this strain of animal (i.e. localized patchy hair loss) were not 

sampled or recorded. 

The intact uterus (ovaries attached) was removed from the 

abdominal cavity and weighed intact for visibly pregnant animals 

only. Corpora lutea were counted and the number per ovary 

recorded. 

The uterus was then opened and the number and location of the 

following were recorded for each uterine horn: 

• live fetuses 

• dead fetuses (recently dead, no significant degeneration) 

• late embryo-fetal deaths (recognizable dead embryo/fetus 

undergoing degeneration, regardless of size) 

• early embryonic deaths (evidence of implantation, but no 

recognizable embryo), or stain-positive 

The placenta associated with each fetus was examined 

macroscopically and weighed. 

For apparently non-pregnant animals, and for apparently empty 

uterine horns, the number of uterine implantation sites were 

checked after staining with ammonium sulphide [modification of 

the Salewski staining technique (Salewski, 1964)]. 

The uteri and ovaries of apparently non-pregnant rabbits were 

retained in neutral buffered 10% formalin and will be discarded at 

the end of the study when authorized by the Study Director. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 25 

 MA-252E-93-23 Final Report

2.14.3. FETAL EXAMINATIONS 

External Examinations 

All live GD 29 fetuses were individually identified within the litter 

and weighed. Each live fetus was given a macroscopic external 

examination for defects (including observation of the palate). 

Dead fetuses without significant necrosis were also examined. 

Late embryo-fetal deaths were examined for abnormalities to the 

extent possible, observed abnormalities were recorded and the 

tissues were discarded. All resorptions were discarded. 

Visceral 

All fetuses were evaluated for soft tissue abnormalities using a 

micro-dissection procedure similar to that described by Staples

(1974) for examination of the torso. A single partial cross-section 

was made through the coronal suture to permit in situ examination 

of the brain in that region. During the examination, the sex of each 

fetus was determined by inspection of the gonads. 

Skeletal 

After evaluation for soft tissue abnormalities each fetus was 

processed for staining with Alizarin Red S. Fetal skeletal 

specimens were evaluated for skeletal abnormalities, including any 

affecting discernable cartilaginous structures, and ossification 

state. 

Recently-Dead Fetuses, Late Embryo-fetal Deaths 

Recently-dead fetuses were processed and examined as for the live 

fetuses. 

Where possible, late embryo-fetal deaths were examined for 

external abnormalities. Any with obvious external abnormalities 

were preserved in neutral buffered formalin for possible future 

examination. All other late resorptions (and early resorptions) 

were discarded. 

2.15. STATISTICAL ANALYSIS 

All statistical analyses were performed using the individual animal (or litter) 

as the basic experimental unit. The following data types were analyzed as 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 26 

 MA-252E-93-23 Final Report

necessary: 

Continuous Data 

• Maternal body weight, absolute weights and changes 

• Gravid uterine adjusted body weights and changes 

• Food consumption 

• Fetal and placental weights per litter 

Discrete Data (Count, Binomial, or Incidence) 

• Litter size 

• Corpora lutea, implantation sites, percent pre- and post-implantation 

loss (resorptions and early/fetal deaths), sex ratio per litter 

• Incidence of fetal external, visceral and skeletal abnormalities per litter 

2.15.1. METHOD OF ANALYSIS 

Mean values of all exposure groups were compared to the mean 

value for the control group at each time interval. 

Continuous Data 

Test article-treated groups were compared to the control using the 

following procedures: If Bartlett's test for variance homogeneity 

(Bartlett, 1937) was not significant at the 1% level, then parametric 

methods were applied. If the F1 approximate test for monotonicity 

(described below) of dose-response was not significant at the 1% 

level, Williams' test (Williams’, 1971, 1972) for a monotonic trend 

was applied. If the F1 test was significant, suggesting that the 

dose-response was not monotone, Dunnett's test (Dunnett, 1955,

1964) was performed instead. If Bartlett's test was significant at 

the 1% level, then logarithmic and square-root transformations 

were tried. If Bartlett's test was still significant, then nonparametric methods were applied to mean ranks. If the H1

approximate test for monotonicity (described below) was not 

significant at the 1% level, Shirley's test for a monotonic trend 

(Shirley, 1977) was applied. If the H1 test was significant, then 

Steel's test (Steel, 1959) was performed instead. 

For all parameters, significant differences between control and test 

article-treated groups are expressed at the 5% (p<0.05), 

1% (p<0.01) or the 0.1% (p<0.001) level. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 27 

 MA-252E-93-23 Final Report

The F1 test is designed to detect significant departure from 

monotonicity of an ordered set of means. The test statistic 

compares the mean square, NMS, for the deviations of the absolute 

means from the maximum likelihood means, calculated under a 

constraint of monotonicity, with the usual error mean square, EMS. 

The null hypothesis is that the true means are monotonically 

ordered. The test statistic is F1 = NMS/EMS which can be 

compared with standard tables of the F-distribution with 1 and 

error degrees of freedom. 

The H1 test is the non-parametric equivalent of the F1 test. The test 

statistic compares the non-monotonicity sums of squares, NRSS, 

for the deviations of the mean ranks from the maximum likelihood 

mean ranks, with the non-parametric equivalent of the error sums 

of squares, ERSS=N(N+1)/12. The test statistic is H1 = 

NRSS/ERSS which can be compared to standard tables of the 

χ

2

-distribution with 1 degree of freedom. 

Discrete Data 

For litter size, corpora lutea, implantation sites, percent pre- and 

post-implantation loss (resorptions and early/fetal deaths) and sex 

ratio per litter, test article-treated groups were compared to the 

control using the following procedures: If the Jonckheere-Terpstra 

test (Jonckheere, 1954) was significant at the 5% level, then the 

direction of the trend was established and one-tailed step-down 

testing in this direction was performed. If the Jonckheere-Terpstra 

test was not significant at the 5% level, then the Kruskal-Wallis 

test (Kruskal and Wallis, 1952, 1953) was applied. If the KruskalWallis test was significant at the 5% level, the test-article treated 

groups were compared to the control using exact Wilcoxon rank 

sum tests (Wilcoxon, 1945); otherwise, no further comparisons 

were made. 

For incidence of fetal external, visceral and skeletal abnormalities 

per litter, test article-treated groups were compared to the control 

using the following procedures: If the Cochran-Armitage test 

(Armitage, 1955) was significant at the 5% level, then the direction 

of the trend was established and one-tailed step-down testing in 

this direction was performed. If the Cochran-Armitage test was 

not significant at the 5% level, then the χ2 test (Armitage et al

SANITIZED
Huntingdon Life Sciences 09-4348 Page 28 

 MA-252E-93-23 Final Report

2002) was applied. If the χ2 test was significant at the 5% level, 

the test-article treated groups were compared to the control using 

Fisher’s Exact tests (Fisher, 1973), otherwise, no further 

comparisons were made. 

For all parameters, significant differences between control and test 

substance-treated groups are expressed at the 5% (p<0.05), 

1% (p<0.01) or the 0.1% (p<0.001) level. 

2.16. MAJOR COMPUTER SYSTEMS 

The following major computer/software systems were utilized at 

Huntingdon Life Sciences, East Millstone, New Jersey. 

System Version Use 

Liberate Reporting 

System 

2.2 Generation of report tables for the clinical 

observations, body weight, food 

consumption, organ weight and fetal 

pathology data in MS Word formats 

Microsoft Word 

and/or Excel 

 Additional formatting of report tables. 

Quasar 1.0 or 

higher 

Statistical analysis of data 

REES Scientific 

Environmental 

Monitoring System 

WIN 1.5 Monitor and record temperature, humidity 

and/or light intensity readings of applicable 

rooms as well as the temperature readings 

of applicable refrigerators and/freezers 

TSI Aerodynamic 

Particle Sizer 

Spectrometer 

2.9 Acquisition of particle size data 

Xybion Path/Tox 

System 

4.2.2 Direct online capture of clinical 

observations, body weights, food 

consumption, organ weights, anatomic and 

fetal pathology data 

2.17. DATA STORAGE 

All raw data, preserved specimens, and retained samples, as well as the 

original study protocol and the original final report will be maintained in 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 29 

 MA-252E-93-23 Final Report

the Archives of the Testing Facility upon completion of the study. The 

Sponsor will determine the final disposition of these materials. 

2.18. REGULATORY REFERENCES 

2.18.1. TEST GUIDELINES 

The design of this study was based on the OECD 414 and US 

EPA 870.3700 guidelines for embryo-fetal toxicity studies. 

2.18.2. GOOD LABORATORY PRACTICES 

This study was conducted in compliance with the United States 

Environmental Protection Agency's Good Laboratory Practices as 

set forth in 40 CFR Part 792 (TSCA) and the Organization for 

Economic Cooperation and Development (OECD) Principles of 

Good Laboratory Practices ENV/MC/CHEM/(98)17. 

2.18.3. ANIMAL WELFARE ACT COMPLIANCE 

This study complied with all appropriate parts of the Animal 

Welfare Act Regulations: 9 CFR Parts 1 and 2 Final Rules, 

Federal Register, Volume 54, No. 168, August 31, 1989, pp. 

36112-36163 effective October 30, 1989 and 9 CFR Part 3 Animal 

Welfare Standards; Final Rule, Federal Register, Volume 56, No. 

32, February 15, 1991, pp. 6426-6505 effective March 18, 1991. 

2.19. PROTOCOL DEVIATION 

The following protocol deviation occurred during the study but was not 

considered to have compromised the validity or integrity of the study: 

The temperature, humidity, liquid flowmeter setting, airflow rate and static 

pressure readings were not recorded for the Group 4 chamber at the 

2.5 hour interval on Exposure Day 13. Values and settings recorded at the 

2.0 and 3.0 hour intervals on this day were within the protocol required 

ranges and were within the required settings. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 30 

 MA-252E-93-23 Final Report

3. RESULTS AND DISCUSSION 

3.1. CHAMBER MONITORING 

(Appendix A) 

Pre-study chamber distribution analyses showed the test substance was 

evenly distributed within each chamber. The target and mean analytical 

(IR) and nominal concentrations are summarized as follows: 

Group 

Target 

Concentration 

(ppm)

Analytical

Concentration 

(ppm) 

Nominal 

Concentration 

(ppm)

1 0 0.0 0

2 2500 2513 NA 

3 10000 9945 NA 

4 15000 14942 30154* 

*the combined nominal concentration for all 3 exposed groups 

The achieved mean exposure concentration for each group was very close to 

the respective target concentration. The differences between measured 

(total of 27400 ppm) and nominal (30154 ppm) concentrations were 

considered representative of minor differences in calibration of the IR 

spectrophotometer and/or the chamber airflows. Chamber environmental 

conditions averaged 22°C and 56% relative humidity. 

Mean particle size distribution measurements for the exposures are 

summarized as follows: 

Group Test 

Substance 

Mass Median 

Aerodynamic 

Diameter 

(μm) 

Geometric 

Standard 

Deviation 

Total 

Mass 

Concentration 

(mg/m3

) 

1 Air Control 6.244 2.117 3.62 x 10-3

2 2.803 1.694 1.59 x 10-3

3 2.697 1.847 1.47 x 10-3

4 1.916 1.618 1.14 x 10-3

These results indicated that the atmospheres were essentially vapor only, as 

expected, since there was no substantial difference between the test 

substance chambers and the air control chamber. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 31 

 MA-252E-93-23 Final Report

3.2. MATERNAL DATA 

3.2.1. MORTALITY 

(Table 1; Appendix B) 

There was no effect of exposure on survival for females at 

exposures up to 15,000 ppm. All females survived until the 

termination of the study. 

3.2.2. CLINICAL OBSERVATIONS 

(Tables 2, 3 and 4; Appendices C and D) 

In-chamber observations were within normal limits on all exposure 

days for all test groups. 

There were no exposure-related findings in the test substance 

exposed animals during the non-exposure intervals. Only 

transient, minor, non-adverse findings were observed in these 

exposure groups. 

3.2.3. GESTATION BODY WEIGHTS 

(Figure 2; Tables 5 and 6; Appendices E and F) 

There were no exposure-related differences in body weights and 

body weight gains in the test substance exposed animals during the 

gestation period as compared with the air control group. 

3.2.4. GESTATION FOOD CONSUMPTION 

(Figure 3; Table 7; Appendix G) 

There were no exposure-related differences in feed consumption in 

the test substance exposed animals, compared to the air control 

animals during the gestation period. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 32 

 MA-252E-93-23 Final Report

3.2.5. MATERNAL MACROSCOPIC POSTMORTEM 

EVALUATIONS 

(Table 8; Appendix H) 

There were no test substance-related macroscopic abnormalities. 

Macroscopic findings were typical of the background findings seen 

in rabbits of this age. 

3.2.6. PREGNANCY OUTCOME AND CESAREAN SECTION 

DATA 

(Tables 9 and 10; Appendix I) 

There were no test substance-related effects on pregnancy status 

and pregnancy rate. 

There was no difference between the groups pertaining to the 

number of corpora lutea, number of implantations, sex ratio 

(% male) or post-implantation losses (%). The increase in preimplantation loss (%) at ≥10,000 ppm was unlikely affected by 

treatment as these parameters were established prior to initiation of 

exposure. 

3.3. FETAL DATA 

3.3.1. GRAVID UTERINE, PLACENTAL AND FETAL WEIGHTS 

(Tables 11 and 12; Appendices J and K) 

There were no test substance-related effects on gravid uterine, 

placental or fetal weights. 

Body weight changes (GD 6 to 29) were reduced at ≥2,500 ppm, 

while adjusted body weight changes were increased when 

compared with control values. This is considered due to the 

exclusions of the non-pregnant animals from group means at the 

end of the study. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 33 

 MA-252E-93-23 Final Report

3.3.2. FETAL OBSERVATIONS 

(Table13; Appendix L) 

There was no evidence of an exposure-related qualitative or 

quantitative increase in the incidences of external, visceral or 

skeletal malformations/variations or ossifications. 

4. CONCLUSION 

There were no effects of exposures on survival, clinical signs, body weights, food 

consumption or maternal or fetal findings at sacrifice. Therefore, the noobserved-effect level (NOEL) for adult pregnant female rabbits for systemic and 

reproductive and developmental toxicity was 15,000 ppm of 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 34 

 MA-252E-93-23 Final Report

REFERENCES 

Armitage, P. 1955. Tests for linear trends in proportions and frequencies. Biometrics 

(Sept.): 375-386. 

Armitage, P., Berry, G. and Matthews, J.N.S. 2002. Statistical Methods in Medical 

Research. 4th edition, p.227. UK: Blackwell Science. 

Bartlett, M.S. 1937. Properties of sufficiency and statistical tests. Proceedings of the 

Royal Society, Series A, 160: 268-282. 

Dunnett, C.W. 1955. A multiple comparison procedure for comparing several treatments 

with a control. Journal of the American Statistical Association 50: 1096-1121. 

Dunnett, C.W. 1964. New tables for multiple comparisons with a control. Biometrics 20-

3: 482-491. 

Fisher, R.A. 1973. Statistical Methods for Research Workers. 14th edition. New York: 

Hafner Publishing Company. 

Jonckheere, A.R. 1954. A distribution-free k-sample test against ordered alternatives. 

Biometrics 41: 133-145. 

Kruskal, W.H. and Wallis, W.A. 1952. Use of Ranks in One-Criterion Variance Analysis. 

Journal of the American Statistical Association, 47, 583-621. 

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis 1952. Journal of the 

American Statistical Association, 48, 907-911. 

Salewski, E. 1964. Farbemethode zum makroskopischen machweis von 

implantationsstellen am uterus der ratte. Archiv. Path. Exp. Pharmakol. 247: 367. 

Shirley, E.A.C. 1977. A non-parametric equivalent of Williams’ test for contrasting 

increasing dose levels of a treatment. Biometrics 33: 386-389. 

Staples, R.E. 1974. Detection of visceral alteration in mammalian fetuses. Teratology 9: 

A37. 

Steel, R.G.D. 1959. A multiple comparison rank sum test: treatments versus control. 

Biometrics 15: 560-572. 

Wilcoxon, F. 1945. Individual comparisons by ranking methods. Biometrics Bulletin 1: 

80-83. 

Williams, D.A. 1971. A test for differences between treatment means when several dose 

levels are compared with a zero dose control. Biometrics 27: 103-117. 

Williams, D.A. 1972. The comparison of several dose levels with a zero dose control. 

Biometrics 28: 519-531. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 35 

 MA-252E-93-23 Final Report 

CALCULATIONS 

Adjusted Body Weight Day 29: 

Body weight on Day 29 – Gravid Uterine Weight 

Adjusted Body Weight Change Days 6 – 29: 

Adjusted body weight Day 29 – Body weight Day 6 

Food Consumption: 

grams of food consumed per interval ÷ number of days = grams/animal/day 

Preimplantation Loss: 

Number of corpora lutea - Number of implantation sites 

Percent Preimplantation Loss: 

Number of corpora lutea - Number of implantation sites/Number of corpora lutea x 100 

Postimplantation Loss: 

Total number of early and late resorptions and dead fetuses 

Percent Postimplantation Loss: 

Number of early/late resorptions and dead fetuses/Number of implantation sites x 100 

Sex Ratio (% Males): 

Number of male pups/number of total pups x 100 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 36 

 MA-252E-93-23 Final Report 

 

General Preface 

General Notes

Individual animal data presented in this report may be rounded. Unrounded individual 

animal data were used to calculate the reported mean and standard deviation values. 

Therefore, use of the reported individual values to reproduce means, standard deviations 

and/or to perform any subsequent calculations may produce minor discrepancies between 

the calculated values and those presented in this report. 

The data collection system used for collecting the in-life and post-life data divides the 

study into phases. The phase utilized on this study was: 

Gestation Phase (GES): Begins the on day of mating (GES 0). Animals 

were exposed to the test material on GES 6 to 28. 

Corresponding exposure levels for each group were as follows: 

Group 1 - 0 ppm 

Group 2 - 2500 ppm 

Group 3 - 10000 ppm 

Group 4 - 15000 ppm 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 37

 MA-252E-93-23 Final Report

Group 1 

Chamber Generation System 

and Whole-Body Exposure Chamber Figure 1 

Note: Sampling Ports H-3 and H-4, used for pretest distribution sampling, were located 

on center of the left wall and Sampling Ports H-9 and H-10 were located on the center of 

the right wall of the chambers. Sampling Ports H-11, H-12, H-13 and H-14 were located 

on the back wall of the chambers. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 38

 MA-252E-93-23 Final Report

Groups 2, 3 and 4 

Chamber Generation System 

and Whole-Body Exposure Chamber Figure 1 

Notes: 

1. Sampling Ports H-3 and H-4, used for pretest distribution sampling, were located 

on center of the left wall and Sampling Ports H-9 and H-10 were located on the 

center of the right wall of the chambers. Sampling Ports H-11, H-12, H-13 and 

H-14 were located on the back wall of the chambers. 

2. The generation system used for Group 4 included 2 liquid flowmeters and 

volatization chambers. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 39

 MA-252E-93-23 Final Report

 

Mean Body Weights Figure 2 

3300

3350

3400

3450

3500

3550

3600

3650

3700

3750

3800

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29

Gestation Day

Body Weight (grams)

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppm

SANITIZED
Huntingdon Life Sciences 09-4348 Page 40

 MA-252E-93-23 Final Report

 

Mean Food Consumption Values Figure 3 

100

105

110

115

120

125

130

135

140

145

150

155

160

165

4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29

Gestation Days

Food Consumption (g/day)

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppm

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Survival 

Dose Group 1 2 3 4 Dose Level (ppm) 0 2500 10000 15000 

 n (%) n (%) n (%) n (%) Number of females entered on study 22 22 22 22 

Removed before scheduled termination 

Died 0 (0) 0 (0) 0 (0) 0 (0) Moribund/elective sacrifice 0 (0) 0 (0) 0 (0) 0 (0) Total 0 (0) 0 (0) 0 (0) 0 (0) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Summary of In-Chamber Observationsa 

aObservations are based on total animals present in the chamber each day. 

All = 100% of animals exhibiting a given observation. 

Exposure Day 1 2 3 4 5 6 7 8 9 10 11 Group 1 – 0 ppm Within Normal Limits All All All All All All All All All All All 

Group 2 – 2500 ppm Within Normal Limits All All All All All All All All All All All Group 3 – 10000 ppm Within Normal Limits All All All All All All All All All All All Group 4 – 15000 ppm Within Normal Limits All All All All All All All All All All All 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Summary of In-Chamber Observationsa 

aObservations are based on total animals present in the chamber each day. 

All = 100% of animals exhibiting a given observation. 

Exposure Day 15 16 17 18 19 20 21 22 23 24 25 Group 1 – 0 ppm Within Normal Limits All All All All All All All All All All All 

Group 2 – 2500 ppm Within Normal Limits All All All All All All All All All All All 

Group 3 – 10000 ppm Within Normal Limits All All All All All All All All All All All 

Group 4 – 15000 ppm Within Normal Limits All All All All All All All All All All All 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 44 

 MA-252E-93-23 Final Report 

Summary of Daily Observations 

Preface Table 3 

Viability observations (cage-side) were performed twice daily (once in the morning and 

once in the afternoon). For summarization purposes, only time-points with animals 

exhibiting abnormal findings were presented. Animals were considered to be within 

normal limits at all other time-points. 

Corresponding exposure levels for each group were as follows: 

Group 1 - 0 ppm 

Group 2 - 2500 ppm 

Group 3 - 10000 ppm 

Group 4 - 15000 ppm 

Key to Abbreviations: 

AM = Morning Viability Observation 

PM = Afternoon Viability Observation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Daily Observations 

 Phase GES 

Day 4 6 9 10 11 12 13 14 15 15 16 16 

Session AM AM AM AM AM AM AM AM AM PM AM PM A

Observations Group General Appearance, Red Exudate (Limbs/Tail) 4 1 1 1 Dermal General, Ulceration (Limbs/Tail) 4 1 1 1 Dermal General, Laceration (Limbs/Tail) 4 1 Daily Observations, Decreased Fecal Volume 1 1 1 

2 1 2 3 2 1 1 1 3 2 4 1 1 1 2 2 2 

Miscellaneous, Red Exudate Source Unknown 3 1 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Daily Observations 

 Phase GES 

Day 18 18 19 19 20 20 21 21 22 22 23 23 2

Session AM PM AM PM AM PM AM PM AM PM AM PM A

Observations Group Dermal General, Ulceration (Limbs/Tail) 4 1 1 1 1 1 1 1 1 1 1 1 Dermal General, Scab(s) 4 1 Daily Observations, Decreased Fecal Volume 1 1 

2 1 1 3 2 2 2 1 1 4 2 2 1 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Daily Observations 

 Phase GES 

Day 25 25 26 26 27 27 28 29 

Session AM PM AM PM AM PM AM AM 

Observations Group General Appearance, Ear Part Missing Unilateral 4 1 1 1 1 1 Dermal General, Alopecia (Head/Neck) 2 1 Dermal General, Scab(s) 4 1 1 1 1 1 1 1 1 Daily Observations, Decreased Fecal Volume 2 1 1 

3 1 1 2 2 1 4 2 2 2 2 

Daily Observations, No Stool 3 1 Miscellaneous, Red Exudate Source Unknown 4 1 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 48 

 MA-252E-93-23 Final Report 

Summary of Physical Examination Findings 

Preface Table 4 

Physical examinations were performed once daily. During the exposure period 

(Gestation Days 6 to 20), these observations were performed after daily exposures. 

Number of animals examined represents the total number of animals observed for a given 

interval. 

For summarization purposes, descriptive comments [i.e., location of scab(s) and/or 

sore(s), etc.] are not presented in this table. These data are contained in the study raw 

data if needed. 

Corresponding exposure levels for each group were as follows: 

Group 1 - 0 ppm 

Group 2 - 2500 ppm 

Group 3 - 10000 ppm 

Group 4 - 15000 ppm 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Physical Examination Findings 

 Phase GES 

Day 1 2 3 4 5 6 7 8 9 10 11 12 

Observations Group Examined 1 10 22 22 22 22 22 22 22 22 22 22 22 2

2 10 22 22 22 22 22 22 22 22 22 22 22 23 12 22 22 22 22 22 22 22 22 22 22 22 24 12 22 22 22 22 22 22 22 22 22 22 22 2

Within Normal Limits 1 10 22 22 21 22 22 22 22 22 22 22 22 2

2 10 21 21 20 20 21 22 22 22 22 21 21 23 12 22 21 20 19 19 20 20 21 21 20 20 24 12 20 18 18 18 18 19 18 18 18 18 18 

General Appearance, Ear Part Missing Unilateral 4 1 1 1 1 1 1 1 1 1 1 1 General Appearance, Red Exudate (Limbs/Tail) 4 Dermal General, Alopecia (Head/Neck) 2 1 1 

3 1 1 4 1 1 1 

Dermal General, Alopecia (Torso) 4 Dermal General, Ano-Genital Staining 2 1 1 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Physical Examination Findings 

 Phase GES 

Day 1 2 3 4 5 6 7 8 9 10 11 12 

Observations Group Dermal General, Ulceration (Limbs/Tail) 4 Dermal General, Abrasion (Head/Neck) 3 1 1 2 2 2 1 

4 1 2 2 2 2 1 1 1 1 1 1 

Dermal General, Abrasion (Torso) 2 1 1 1 1 1 Dermal General, Stains on Fur (Torso) 2 1 1 Dermal General, Scab(s) 1 1 

3 1 1 1 1 1 1 1 1 4 1 2 2 2 2 2 3 3 3 2 2 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Physical Examination Findings 

 Phase GES 

Day 16 17 18 19 20 21 22 23 24 25 26 27 2

Observations Group Examined 1 22 22 22 22 22 22 22 22 22 22 22 22 2

2 22 22 22 22 22 22 22 22 22 22 22 22 23 22 22 22 22 22 22 22 22 22 22 22 22 24 22 22 22 22 22 22 22 22 22 22 22 22 2

Within Normal Limits 1 22 22 22 22 22 22 22 22 22 22 22 21 2

2 21 21 21 21 21 21 21 20 20 20 20 20 23 21 21 21 21 20 21 21 21 21 21 21 21 24 17 17 17 17 17 17 17 16 16 16 17 16 

General Appearance, Ear Part Missing Unilateral 4 1 1 1 1 1 1 1 1 1 1 1 1 General Appearance, Red Exudate Ano-Genital Area 4 General Appearance, Red Exudate (Limbs/Tail) 4 1 1 Dermal General, Alopecia (Limbs) 1 1 

2 

SANITIZED 3 
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Physical Examination Findings 

 Phase GES 

Day 16 17 18 19 20 21 22 23 24 25 26 27 2

Observations Group Dermal General, Alopecia (Head/Neck) 2 1 1 1 1 1 1 1 1 1 1 1 1 

3 1 1 1 1 1 1 1 1 1 1 1 1 4 2 2 2 2 1 1 1 2 2 2 1 2 

Dermal General, Alopecia (Torso) 1 

2 1 1 1 1 1 4 1 1 1 1 1 1 1 1 1 1 1 1 

Dermal General, Ano-Genital Staining 4 Dermal General, Ulceration (Limbs/Tail) 4 1 1 1 1 1 1 1 Dermal General, Scab(s) 3 1 

4 1 1 1 1 1 1 1 2 2 2 2 2 

Miscellaneous, Red Exudate Source Unknown 4 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Mean Body Weights 

Females (grams) 

 Phase GES  Day 4 6 9 12 15 18 20 23 26 29 Group 1 - 0 ppm Mean 3327.7 3390.4 3397.8 3428.0 3499.9 3512.8 3558.9 3613.4 3658.3 3702.7 SD 264.03 262.60 293.38 303.32 308.02 286.20 304.93 313.10 307.56 308.81 N 22 22 22 22 22 22 22 22 22 22 

Group 2 - 2500 ppm 

Mean 3371.0 3431.8 3445.6 3475.6 3535.8 3555.5 3587.0 3625.5 3662.3 3693.3 SD 205.98 221.89 215.41 217.60 208.41 221.49 222.69 236.83 235.96 226.93 

N 21 21 21 21 21 21 21 21 21 21 

Group 3 - 10000 ppm 

Mean 3367.5 3410.4 3399.0 3400.1 3453.6 3477.1 3500.3 3561.0 3592.7 3614.6 SD 312.54 301.03 288.77 297.00 276.99 262.25 264.58 265.27 267.23 284.11 

N 20 20 20 20 20 20 20 20 20 20 

Group 4 - 15000 ppm 

Mean 3462.1 3526.5 3513.6 3531.2 3608.1 3626.1 3638.8 3681.9 3728.3 3747.6 SD 225.83 232.00 232.82 232.80 246.11 240.50 244.52 252.01 248.98 288.19 

N 22 22 22 22 22 22 22 22 22 22 No statistically significant differences from control mean 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Mean Body Weight Changes 

Females (grams) 

 Phase GES  Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 1 - 0 ppm Mean 62.7 7.3 30.2 71.9 12.9 46.1 54.6 44.9 44.4 168.5 SD 52.74 56.58 43.28 49.36 90.15 71.27 38.83 42.08 38.98 105.24 N 22 22 22 22 22 22 22 22 22 22 

Group 2 - 2500 ppm 

Mean 60.8 13.8 30.0 60.2 19.7 31.6 38.5 36.7 31.0 155.3 SD 63.58 46.68 44.07 46.89 57.48 30.81 73.33 37.80 44.66 118.70 

N 21 21 21 21 21 21 21 21 21 21 

Group 3 - 10000 ppm 

Mean 42.9 -11.4 1.1 53.5 23.5 23.3 60.6 31.7 21.9 90.0 SD 46.00 46.11 46.11 87.25 52.03 32.71 31.89 52.92 54.37 167.86 

N 20 20 20 20 20 20 20 20 20 20 

Group 4 - 15000 ppm 

Mean 64.4 -12.9 17.6 76.9 18.1 12.7 43.1 46.5 19.3 112.3 SD 54.05 53.91 33.75 65.05 34.64 39.92 36.71 59.91 146.79 123.37 

N 22 22 22 22 22 22 22 22 22 22 No statistically significant differences from control mean 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Mean Food Consumption Values 

Females (grams/day) 

 Phase GES  Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 1 - 0 ppm Mean 150.3 149.0 149.6 134.4 143.9 161.2 149.6 131.5 128.1 146.6 SD 42.79 38.03 36.69 40.66 44.69 36.59 36.79 36.40 30.14 35.33 N 22 22 22 22 22 22 22 22 22 22 

Group 2 - 2500 ppm 

Mean 152.0 149.5 144.5 128.0 142.9 157.7 144.2 119.4 120.2 143.6 SD 36.10 33.31 36.41 36.32 46.61 36.43 35.02 40.06 41.45 33.61 

N 21 21 21 21 21 21 21 21 21 21 

Group 3 - 10000 ppm 

Mean 142.4 131.5 124.3 106.2 120.5 132.4 129.6 114.5 115.9 122.5 SD 33.80 33.32 36.76 49.59 54.23 47.67 46.01 43.28 44.04 39.80 

N 20 20 20 20 20 20 20 20 20 20 

Group 4 - 15000 ppm 

Mean 155.7 138.8 139.7 126.4 140.2 151.3 142.7 125.2 127.5 138.4 SD 33.15 36.22 38.78 48.22 47.47 47.45 41.55 48.65 47.16 41.56 

N 22 22 22 22 22 22 22 22 22 22 No statistically significant differences from control mean 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 56 

 MA-252E-93-23 Final Report

 Incidence Summary Report 

for Gross Necropsy Observations 

Preface Table 8 

Key to Abbreviations

Oviducts/Fallop = Oviducts/Fallopian 

w/ = with 

Corresponding exposure levels for each group were as follows: 

Group 1 - 0 ppm 

Group 2 - 2500 ppm 

Group 3 - 10000 ppm 

Group 4 - 15000 ppm 

SANITIZED
 Huntingdon Life Sciences Incidence Summary Report for Gross Necropsy Observations by Animal 

 Mettlers Road Study number: 09-4348  P.O. Box 2360 All Sacrifices  Rabbit/New Zealand White Study start date: 08-Mar-10 Se ________________________________________________________________________________________________________________ -- Females --  Group: 1 2 3 4  Number in group: 22 22 22 22  ---------------------------------------------------------------------------------------------------------------- Within Normal Limits .............. 21 20 20 19  Oviducts/Fallop  Cyst ........................... 0 2 0 0  Placenta  Abnormal Contents .............. 0 0 1 0  Discolored ..................... 1 0 0 2  Enlarged ....................... 0 0 0 1  Small .......................... 0 0 0 1  Spleen  Small .......................... 0 0 0 1  Uterus w/ Cervix  Absent ......................... 0 0 1 0 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Summary of Pregnancy 

Dose Group 1 2 3 4 Dose Level (ppm) 0 2500 10000 15000 

 n (%) n (%) n (%) n (%) Number of females entered on study (mated) 22 22 22 22 

- Non pregnant 0 (0) 1 (5) 2 (9) 0 (0) 

- Pregnant 22 (100) 21 (95) 20 (91) 22 (100) 

Pregnant with termination before scheduled date 0 0 0 0 

Pregnant at scheduled termination 22 21 20 22 

- With total implantation loss 0 (0) 0 (0) 0 (0) 0 (0) 

- With viable fetuses 22 (100) 21 (100) 20 (100) 22 (100) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Summary of Cesarean Section Data 

 Corpora Implant- Resorptions Dead Live Fetuses Sex Rat Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 1 - 0 ppm Mean 10.0 9.5 0.5 0.1 0.6 0.0 4.5 4.4 8.9 51.1 SD 1.93 1.95 0.86 0.29 0.96 0.00 1.84 2.17 1.81 20.6

N 22 22 22 22 22 22 22 22 22 22 

Group 2 - 2500 ppm Mean 9.2 8.8 0.1 0.1 0.2 0.0 4.1 4.4 8.5 48.7 SD 1.67 1.87 0.36 0.30 0.44 0.00 1.80 1.86 1.78 19.9

N 21 21 21 21 21 21 21 21 21 21 

Group 3 - 10000 ppm Mean 10.2 8.6 0.3 0.2 0.5 0.0 4.1 4.1 8.1 51.7 SD 2.16 2.33 0.44 0.52 0.69 0.00 2.06 2.35 2.29 23.6

N 20 20 20 20 20 20 20 20 20 20 

Group 4 - 15000 ppm Mean 10.4 9.2 0.2 0.1 0.4 0.2 4.4 4.2 8.6 52.1 SD 1.32 1.84 0.54 0.36 0.59 1.09 2.04 2.25 2.04 20.3

N 21 21 21 21 21 21 21 21 21 21 

Impl. = Implantation * = p < 0.05 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Mean Gravid Uterine Adjusted Body Weights 

Females (grams) 

 Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 1 - 0 ppm 

Mean 3390 3703 312 504.52 3198 -192 

SD 263 309 127 86.445 277 110 

N 22 22 22 22 22 22 

Group 2 - 2500 ppm 

Mean 3432 3693 262 487.06 3206 -226 

SD 222 227 172 83.754 200 154 

N 21 21 21 21 21 21 

Group 3 - 10000 ppm 

Mean 3410 3615 204 468.79 3146 -265 

SD 301 284 213 94.317 290 213 

N 20 20 20 20 20 20 

Group 4 - 15000 ppm 

Mean 3516 3777 261 530.42 3246 -270 

SD 232 260 138 76.468 251 164 

N 21 21 21 21 21 21 

Adj = Adusted, Bwt = Bodyweight No statistically significant differences from control mean 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Mean Litter Weights 

 (grams) 

 Placental Total Litter Male Fetal Female Fetal  Weight Weight Weight Weight Group 1 - 0 ppm Mean 4.8 345.7 39.7 38.2 SD 0.51 61.12 4.11 5.34 N 22 22 22 22 

Group 2 - 2500 ppm Mean 4.9 330.4 39.7 39.1 SD 0.54 62.33 4.16 4.92 

N 21 21 21 21 

Group 3 - 10000 ppm Mean 5.1 314.1 40.0 39.5 SD 0.74 73.41 6.10 5.74 

N 20 20 20 19 

Group 4 - 15000 ppm Mean 5.1 356.8 42.2 40.2 SD 0.54 53.92 4.80 4.65 

N 21 21 21 21 No statistically significant differences from control mean 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 62 

 MA-252E-93-23 Final Report

Summary of Fetal Observations 

Preface Table 13 

External Examinations .......................................................................................................63

Visceral Examinations .......................................................................................................64

Skeletal Examinations ........................................................................................................67

Ossification Parameters .....................................................................................................71

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 External Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation External Examinations 195 179 162 186 22 Abdomen/trunk  Abdomen, gastroschisis 1  Tail, rudimentary 1  Umbilicus, hernia 1 Paws/limbs  Forelimb(s), flexure(s) 4  Forelimb(s), lower, upper, thickened 1  Forepaw(s), digit(s), second, misshapen 1  Forepaw(s), flexure(s) 3  Hindlimb(s), flexure(s) 5  Left forepaw, digit(s), third, misshapen 1 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Visceral Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Visceral Examinations 195 179 162 186 22 Abdomen  Abdominal cavity, fluid filled 1  Jejunum, cyst 1  Spleen, small 1 Brain  Lateral ventricle, dilated 1 1 Lateral ventricle, hydrocephaly 1 1Cervical  Thymus gland, enlarged 1  Thymus gland, partially undescended 2 2 4  Thymus gland, partially undescended, left side 1 3 2 1 1  Thymus gland, partially undescended, right side 2 4  Thymus gland, small 1 Eye  Eye, small 1 Heart  Atrium, distended 1 2 1  Atrium, enlarged 1  Atrium, irregular 1 †Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence. 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Visceral Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Visceral Examinations 195 179 162 186 22 Heart  Bilateral ventricles, enlarged 1  Heart, malrotated 1  Heart, small 5  Papillary muscle, rudimentary 1  Pericardium, distended 1  Ventricle, av-valve, nodule 1  Ventricle, small 1  Ventricular septum, small, membranous, ventricular septal defect 1 Liver  All lobes, enlarged 1  Gallbladder, absent 1  Gallbladder, rudimentary 3 1 4 1 3 Lungs  Accessory lung lobe, absent 1 8 2 1 1  Accessory lung lobe, rudimentary 1 1 1 1  Accessory lung lobe, small 1 1 1 1  Left lung lobe, cyst 1  Lungs (general), rudimentary 1  Lungs (general), small 1 1 av = atrio-ventricular †Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence. 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Visceral Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Visceral Examinations 195 179 162 186 22 Thorax  Aorta, distended 1  Aortic arch, interrupted 1 1 Caudal vena cava, distended, malpositioned 1  Common carotid artery, branching variation, left side 1 

 Common carotid artery, displaced, left side 4 3 Subclavian artery, branching variation, left side 5 4 2 6 3  Subclavian artery, left side, additional 6 2 5 8 5  Thoracic cavity, fluid filled 1 

Whole body  Skin/subcutis, edema 1  Lumbar spine, musculature, enlarged 1 

†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence. 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Skeletal Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Caudal vert.  Caudal vertebra(e), absent 1  Caudal vertebra(e), fused 1 Cervical vert.  Cervical rib, complete with costal cart. 1  Cervical rib, without costal cartilage 1 2 1 1 Girdles  Clavicle, bent 2 1 4 6 1  Clavicle, enlarged 1  Clavicle, thickened 1  Pelvic girdle, unilateral caudal shift 3 2 1 3  Pelvic girdle, unilateral cranial shift 2 2 2  Scapula, enlarged 1 Head  Frontal, fissure(s) 1  Frontal, suture within, partially fused 1  Frontal, unossified area(s) 10 7 6 1 7  Hyoid cornua, bent inwards 11 7 5 8 5  Hyoid cornua, bent outwards 1 2 1 1 1  Interparietal, supraoccipital, bridge of ossification 2 2 1  Interparietal, supraoccipital, fused 1 2  Interparietal, supraoccipital, partially fused 1 1vert. = vertebra(e) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Skeletal Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Head  Nasal, suture within 1  Parietal, fissure(s) 2 1  Parietal, unossified area(s) 4 3 2 2 Limb bones  Humerus, thickened 1  Metacarpal, thickened 1  Radius, thickened 1  Ulna, misshapen, thickened 1  1st phalanx, thickened 1 Lumbar vert.  Lumbar region, scoliosis 1 1 Lumbar transverse process, enlarged 1  Lumbar vertebral arch(es), absent 1 1 Lumbar vertebral arch(es), misaligned 1 1 Lumbar vertebral centrum(a), fused 1  Lumbar vertebral centrum(a), misaligned 1 1Ribs  13th, interrupted 1  8th, branched 1  Distal, nodulated 1 vert. = vertebra(e) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Skeletal Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Sternebrae  Sternebral center(s), bridge of ossification 7 1 3 3 1  Sternebral center(s), cranial to, supernumerary ossification 2 1 1  Sternebral center(s), fused 1 1 1  Sternebral center(s), misaligned 1 3  Sternebral center(s), supernumerary ossification 1 1 Sternebral center(s), supernumerary ossification, between 1 1  Sternebral center(s), supernumerary ossification, partially fused, between 1 Suture/fontanel  Anterior fontanelle, enlarged 3 3 2 3  Frontal/parietal suture, irregular 1  Frontal suture, plaque of bone 4 2 9 3  Frontal suture, sutural bone 1 

 Nasal suture, sutural bone 1 1 1 1 1  Parietal suture, enlarged 1 

 Parietal suture, irregular 2 2 

 Parietal suture, plaque of bone 2  Parietal suture, sutural bone 4 4 

 Posterior fontanelle, enlarged 4 1 2 2 Thoracic vert.  18 thoracolumbar vertebrae, present 1  20 thoracolumbar vertebrae, present 6 9 6 11 6  Thoracic hemicentrum(a), fused 1 

vert. = vertebra(e) 

†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence. 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Skeletal Examinations 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Thoracic vert.  Thoracic vert. centrum(a), bipartite 1  Thoracic vert. centrum(a), misshapen 1  Thoracic vertebral arch(es), fused 1  Thoracic vertebral arch(es), small 1 Variants  13th bilateral rib, complete 53 52 48 54 14  13th bilateral rib, rudimentary 3  13th bilateral rib, short 12 8 13 11 7  13th bilateral rib, short, non-articulated 1  13th left rib, complete 16 18 9 22 10  13th left rib, rudimentary 1 1 1 1  13th left rib, rudimentary, non-articulated 3 2 5 3  13th left rib, short 11 15 8 15 10  13th left rib, short, non-articulated 1 4 2 4 1  13th right rib, complete 10 9 5 18 7  13th right rib, rudimentary 3 1 3 2 3  13th right rib, rudimentary, non-articulated 1 2 1 1  13th right rib, short 25 17 7 31 15  13th right rib, short, floating 1  13th right rib, short, non-articulated 2 2 1 4 2 vert. = vertebra(e) †Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence. 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Ossification Parameters 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Caudal vert.  Caudal vertebra centrum(a), bipartite ossified 1  Caudal vertebra(e), asymmetrically ossified 1 Cervical vert.  Cervical hemicentrum(a), unossified 1  Odontoid process, incompletely ossified 3 1 4 2 2  Odontoid process, ossified 1  Odontoid process, unossified 1 Girdles  Pubis, incompletely ossified 1 1 1 1  Pubis, unossified 1 Head  Anterior supraorbital notch, incompletely ossified 6 4 10 2 1  Hyoid body, incompletely ossified 1  Hyoid body, unossified 3 1 8 3 1  Hyoid cornua, unossified 1  Interparietal, incompletely ossified 3 3  Jugal, incompletely ossified 1  Premaxilla, incompletely ossified 1 2 1 Limb bones  Metacarpal, incompletely ossified 27 20 30 34 13 vert. = vertebra(e) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Ossification Parameters 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Limb bones  Metacarpal, unossified 16 12 14 4 9  Talus, incompletely ossified 2 1 1 1  Talus, unossified 1 1 1  1st phalanx, bilateral forelimb, unossified 1  2nd phalanx, bilateral, unossified 1 1 1  2nd phalanx, bilateral forelimb, incompletely ossified 36 44 24 45 17  2nd phalanx, bilateral forelimb, unossified 31 68 64 59 13  2nd phalanx, bilateral hind limb, incompletely ossified 3 10 13 7 3  2nd phalanx, bilateral hind limb, unossified 5 5 5  2nd phalanx, left forelimb, incompletely ossified 8 2 11 9 6  2nd phalanx, left forelimb, unossified 3 6 6 4 3  2nd phalanx, right forelimb, incompletely ossified 2 10 7 8 2  2nd phalanx, right forelimb, unossified 2 2 4 5 2  2nd phalanx, right hind limb, incompletely ossified 1 1  Epiphyses, incompletely ossified 26 28 22 20 12  Epiphyses, unossified 7 4 3 3 6  Phalanx, bilateral forelimb, incompletely ossified 1 3 1 

 Phalanx, bilateral forelimb, unossified 1  Phalanx, incompletely ossified 1 

 Phalanx, unossified 1 Sternebrae  Omosternum, ossified 2 2

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Summary of Fetal Observations 

 Ossification Parameters 

Fetuses Litters 

 Group 1 2 3 4 1 Observation Skeletal Examinations 195 179 162 186 22 Sternebrae  Sternebral center(s), bipartite ossified 1 2 1 Thoracic vert.  Thoracic vert. centrum(a), asymmetrically ossified 2  Thoracic vert. centrum(a), bipartite ossified 1  Thoracic vert. centrum(a), dumbbell ossified 1  Thoracic vert. centrum(a), incompletely ossified 1 Variants  Sternebra(e), incompletely ossified 29 30 31 28 13  Sternebra(e), unossified 22 46 47 35 9 vert. = vertebra(e) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration 

Group 1 - 0 ppm 

Day Date 

Exposure 

Number Nominal

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

1 14-Mar-10 1 0 0.0 0.0 0.0 0.0 0.0 2 15-Mar-10 2 0 0.0 0.0 0.0 0.0 0.0 3 16-Mar-10 3 0 0.0 0.0 0.0 0.0 0.0 4 17-Mar-10 4 0 0.0 0.0 0.0 0.0 0.0 1.965 1.883 2.63E-03 5 18-Mar-10 5 0 0.0 0.0 0.0 0.0 0.0 6 19-Mar-10 6 0 0.0 0.0 0.0 0.0 0.0 7 20-Mar-10 7 0 0.0 0.0 0.0 0.0 0.0 8 21-Mar-10 8 0 0.0 0.0 0.0 0.0 0.0 9 22-Mar-10 9 0 0.0 0.0 0.0 0.0 0.0 

10 23-Mar-10 10 0 0.0 0.0 0.0 0.0 0.0 11 24-Mar-10 11 0 0.0 0.0 0.0 0.0 0.0 2.418 1.845 1.11E-03 12 25-Mar-10 12 0 0.0 0.0 0.0 0.0 0.0 13 26-Mar-10 13 0 0.0 0.0 0.0 0.0 0.0 14 27-Mar-10 14 0 0.0 0.0 0.0 0.0 0.0 15 28-Mar-10 15 0 0.0 0.0 0.0 0.0 0.0 16 29-Mar-10 16 0 0.0 0.0 0.0 0.0 0.0 17 30-Mar-10 17 0 0.0 0.0 0.0 0.0 0.0 18 31-Mar-10 18 0 0.0 0.0 0.0 0.

SANITIZED 0 0.0 3.561 1.978 2.09E-03 
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration 

Group 1 - 0 ppm 

Day Date 

Exposure 

Number Nominal

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

19 1-Apr-10 19 0 0.0 0.0 0.0 0.0 0.0 20 2-Apr-10 20 0 0.0 0.0 0.0 0.0 0.0 21 3-Apr-10 21 0 0.0 0.0 0.0 0.0 0.0 22 4-Apr-10 22 0 0.0 0.0 0.0 0.0 0.0 23 5-Apr-10 23 0 0.0 0.0 0.0 0.0 0.0 17.03 2.763 8.65E-03 24 6-Apr-10 24 0 0.0 0.0 0.0 0.0 0.0 25 7-Apr-10 25 0 0.0 0.0 0.0 0.0 0.0 26 8-Apr-10 26 0 0.0 0.0 0.0 0.0 0.0 

Mean 0 0.0 6.244 2.117 3.62E-03 

S.D. 0 0.0 7.222 0.434 3.41E-03 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not a*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results. 

Group 2 - 2500 ppm 

Day Date 

Exposure 

Number Nominal*

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

1 14-Mar-10 1 NA 2100 1100 2700 2200 2500 2 15-Mar-10 2 NA 2500 3100 2200 2800 2000 3 16-Mar-10 3 NA 2500 3800 2000 2000 2000 4 17-Mar-10 4 NA 2400 2400 2400 2800 2100 1.304 1.513 1.58E-03 5 18-Mar-10 5 NA 2600 2600 2900 2400 2300 6 19-Mar-10 6 NA 2400 2500 2500 2400 2200 7 20-Mar-10 7 NA 2500 2500 2500 2100 3000 8 21-Mar-10 8 NA 2500 2500 2600 2500 2300 9 22-Mar-10 9 NA 2400 1800 2700 2600 2500 

10 23-Mar-10 10 NA 2400 2200 2500 2300 2400 11 24-Mar-10 11 NA 2500 2500 2300 2600 2400 2.014 1.796 9.13E-04 12 25-Mar-10 12 NA 2600 3000 2700 2300 2400 13 26-Mar-10 13 NA 2800 3200 2800 2800 2500 14 27-Mar-10 14 NA 2700 2900 2900 2300 2600 15 28-Mar-10 15 NA 2600 2400 2100 3100 2700 16 29-Mar-10 16 NA 2500 2600 2600 2400 2400 17 30-Mar-10 17 NA 2500 2900 2500 2200 2200 18 31-Mar-10 18 NA 2700 2700 2700 3000 2400 6.359 1.709 2.01E-03 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not a*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results. 

Group 2 - 2500 ppm 

Day Date 

Exposure 

Number Nominal*

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

19 1-Apr-10 19 NA 2500 2400 2300 2400 2800 20 2-Apr-10 20 NA 2500 2600 2400 2400 2600 21 3-Apr-10 21 NA 2500 2800 2600 2400 2100 22 4-Apr-10 22 NA 2800 3000 2900 2800 2600 23 5-Apr-10 23 NA 2700 3000 2700 2400 2500 1.535 1.759 1.84E-03 24 6-Apr-10 24 NA 2700 2500 2600 2700 2900 25 7-Apr-10 25 NA 2300 2500 2000 2300 2300 26 8-Apr-10 26 NA 2500 2500 2200 2600 2600 

 Mean NA 2513 2.803 1.694 1.59E-03  S.D. 337 2.389 0.126 4.82E-04 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not a*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results. 

Group 3 - 10000 ppm 

Day Date 

Exposure 

Number Nominal*

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

1 14-Mar-10 1 NA 9800 10000 9300 10000 9900 2 15-Mar-10 2 NA 10000 10000 9800 10000 10000 3 16-Mar-10 3 NA 10000 9800 11000 10000 10000 4 17-Mar-10 4 NA 10000 11000 10000 10000 10000 1.675 1.756 2.00E-03 5 18-Mar-10 5 NA 10000 10000 11000 10000 10000 6 19-Mar-10 6 NA 9900 9700 10000 10000 10000 7 20-Mar-10 7 NA 10000 9900 10000 11000 10000 8 21-Mar-10 8 NA 9800 9700 9600 10000 10000 9 22-Mar-10 9 NA 9600 10000 9400 9600 9500 

10 23-Mar-10 10 NA 9700 9500 9300 10000 10000 11 24-Mar-10 11 NA 10000 10000 10000 10000 10000 2.159 1.805 8.37E-04 12 25-Mar-10 12 NA 10000 10000 10000 10000 10000 13 26-Mar-10 13 NA 9900 10000 9600 9800 10000 14 27-Mar-10 14 NA 9800 10000 9900 9300 9800 15 28-Mar-10 15 NA 10000 10000 9900 9900 10000 16 29-Mar-10 16 NA 10000 11000 10000 10000 10000 17 30-Mar-10 17 NA 10000 10000 10000 9900 10000 18 31-Mar-10 18 NA 9700 9600 9900 9300 10000 5.498 1.838 1.37E-03 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not a*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results. 

Group 3 - 10000 ppm 

Day Date 

Exposure 

Number Nominal*

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

19 1-Apr-10 19 NA 10000 10000 10000 10000 10000 20 2-Apr-10 20 NA 10000 10000 10000 10000 10000 21 3-Apr-10 21 NA 10000 10000 9900 11000 10000 22 4-Apr-10 22 NA 9900 10000 9900 10000 9800 23 5-Apr-10 23 NA 9800 9700 9700 9800 9800 1.454 1.989 1.69E-03 24 6-Apr-10 24 NA 10000 10000 10000 10000 10000 25 7-Apr-10 25 NA 9800 10000 9400 9700 10000 26 8-Apr-10 26 NA 9700 9400 9900 9400 10000 

 Mean NA 9945 2.697 1.847 1.47E-03  S.D. 328 1.891 0.100 4.97E-04 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration. *Nominal concentration calculated for all 3 treated groups combined. 

Group 4 - 15000 ppm 

Day Date 

Exposure 

Number Nominal*

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

1 14-Mar-10 1 31000 15000 15000 15000 15000 15000 2 15-Mar-10 2 30000 15000 15000 14000 15000 15000 3 16-Mar-10 3 30000 15000 14000 15000 15000 15000 4 17-Mar-10 4 30000 16000 16000 15000 16000 15000 1.176 1.530 1.36E-03 5 18-Mar-10 5 30000 15000 15000 16000 15000 15000 6 19-Mar-10 6 29000 15000 15000 15000 15000 15000 7 20-Mar-10 7 30000 15000 14000 14000 15000 15000 8 21-Mar-10 8 30000 15000 15000 15000 16000 14000 9 22-Mar-10 9 29000 14000 15000 14000 14000 14000 

10 23-Mar-10 10 30000 15000 15000 15000 15000 15000 11 24-Mar-10 11 31000 15000 14000 15000 15000 15000 1.889 1.576 9.64E-04 12 25-Mar-10 12 31000 15000 14000 15000 15000 15000 13 26-Mar-10 13 31000 15000 15000 15000 15000 15000 14 27-Mar-10 14 31000 15000 15000 15000 14000 15000 15 28-Mar-10 15 31000 15000 14000 15000 15000 14000 16 29-Mar-10 16 31000 15000 16000 15000 15000 15000 17 30-Mar-10 17 30000 15000 15000 15000 14000 15000 18 31-Mar-10 18 30000 16000 16000 15000 16000 15000 3.271 1.797 9.63E-04 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Chamber Monitoring Results 

Cumulative Exposure Record 

GSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration. *Nominal concentration calculated for all 3 treated groups combined. 

Group 4 - 15000 ppm 

Day Date 

Exposure 

Number Nominal*

Analytical Chamber Concentration

Particle Size 

Determinations 

C

Mean Individual MMAD GSD TMC Tem

(ppm) (ppm) (ppm) (μm) (mg/m3) 

19 1-Apr-10 19 29000 15000 15000 15000 15000 16000 20 2-Apr-10 20 30000 15000 15000 15000 15000 15000 21 3-Apr-10 21 30000 15000 15000 15000 15000 15000 22 4-Apr-10 22 30000 16000 16000 16000 15000 15000 23 5-Apr-10 23 29000 15000 14000 15000 15000 15000 1.326 1.567 1.28E-03 24 6-Apr-10 24 30000 15000 15000 15000 15000 15000 25 7-Apr-10 25 30000 15000 15000 15000 15000 14000 26 8-Apr-10 26 31000 15000 15000 15000 15000 15000 

 Mean 30154 14942 1.916 1.618 1.14E-03  S.D. 675 499 0.954 0.121 2.08E-04 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 82

 MA-252E-93-23 Final Report

 

Females Individual Animal Termination History Appendix B 

Animal Date of Phase 

Number Death Type of Death Phase Daya

 

Group 1 - 0 ppm 

 

1551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1556 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1567 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

1572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

 

 

a

First day of test article administration defined as Gestation Day 6 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 83

 MA-252E-93-23 Final Report

 

Females Individual Animal Termination History Appendix B 

Animal Date of Phase 

Number Death Type of Death Phase Daya

 

Group 2 - 2500 ppm 

 

2551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2556 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2567 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

2572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

 

a

First day of test article administration defined as Gestation Day 6 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 84

 MA-252E-93-23 Final Report

 

Females Individual Animal Termination History Appendix B 

Animal Date of Phase 

Number Death Type of Death Phase Daya

 

Group 3 - 10000 ppm 

 

3551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3556 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3567 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

3572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

 

a

First day of test article administration defined as Gestation Day 6 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 85

 MA-252E-93-23 Final Report

 

Females Individual Animal Termination History Appendix B 

Animal Date of Phase 

Number Death Type of Death Phase Daya

 

Group 4 - 15000 ppm 

 

4551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4556 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4567 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

4572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29 

 

a

First day of test article administration defined as Gestation Day 6 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 86 

 MA-252E-93-23 Final Report 

Individual Daily Observations 

Preface Appendix C 

Viability observations (cage-side) were performed twice daily (once in the morning and 

once in the afternoon). For summarization purposes, only time-points with animals 

exhibiting abnormal findings were presented. Animals were considered to be within 

normal limits at all other time-points. 

Key to Abbreviations: 

AM = Morning Viability Observation 

PM = Afternoon Viability Observation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Daily Observations 

 Phase GES Animal Day 4 6 9 10 11 12 13 14 15 15 1Number Observations Session AM AM AM AM AM AM AM AM AM PM AGroup 1 - 0 ppm 1553 Daily observations, decreased fecal volume P 

1557 Daily observations, decreased fecal volume P 1564 Daily observations, decreased fecal volume Group 2 - 2500 ppm 2554 Daily observations, decreased fecal volume P 2557 Daily observations, decreased fecal volume P P 2563 Daily observations, decreased fecal volume Group 3 - 10000 ppm 3551 Daily observations, decreased fecal volume P 

3555 Daily observations, decreased fecal volume P 

3560 Daily observations, decreased fecal volume P 

P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Daily Observations 

 Phase GES Animal Day 4 6 9 10 11 12 13 14 15 15 1Number Observations Session AM AM AM AM AM AM AM AM AM PM AGroup 3 - 10000 ppm 3561 Daily observations, decreased fecal volume P P P P P 

3570 Daily observations, decreased fecal volume P P 

Miscellaneous, red exudate source unknown P 

Group 4 - 15000 ppm 4551 General appearance, red exudate (limbs/tail), tail P P 

Dermal general, ulceration (limbs/tail), tail P P Dermal general, laceration (limbs/tail), tail 

4554 Daily observations, decreased fecal volume P 

4565 Daily observations, decreased fecal volume P 

4567 Daily observations, decreased fecal volume P P P P 

4569 Daily observations, decreased fecal volume P P P P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Daily Observations 

 Phase GES Animal Day 18 18 19 19 20 20 21 21 22 22 2Number Observations Session AM PM AM PM AM PM AM PM AM PM AGroup 1 - 0 ppm 1564 Daily observations, decreased fecal volume P 

Group 2 - 2500 ppm 2563 Daily observations, decreased fecal volume P P 2569 Daily observations, decreased fecal volume 

Group 3 - 10000 ppm 3555 Daily observations, decreased fecal volume P 3561 Daily observations, decreased fecal volume P P 

3570 Daily observations, decreased fecal volume P P P P P Group 4 - 15000 ppm 4551 Dermal general, ulceration (limbs/tail), tail P P P P P P P P P P P 

Dermal general, scab(s), tail 

4561 Daily observations, decreased fecal volume 

P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Daily Observations 

 Phase GES Animal Day 18 18 19 19 20 20 21 21 22 22 2Number Observations Session AM PM AM PM AM PM AM PM AM PM AGroup 4 - 15000 ppm 4567 Daily observations, decreased fecal volume P P 

4569 Daily observations, decreased fecal volume P P P 

P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Daily Observations 

 Phase GES Animal Day 25 25 26 26 27 27 28 29 Number Observations Session AM PM AM PM AM PM AM AM Group 2 - 2500 ppm 2555 Dermal general, alopecia (head/neck) 3 

Daily observations, decreased fecal volume P 

2557 Daily observations, decreased fecal volume P Group 3 - 10000 ppm 3554 Daily observations, decreased fecal volume P P 

Daily observations, no stool P 

3555 Daily observations, decreased fecal volume P 

3566 Daily observations, decreased fecal volume P P P P 

Group 4 - 15000 ppm 4551 Dermal general, scab(s), tail P P P P P P P P 

4557 General appearance, ear part missing unilateral P P P P P 

Daily observations, decreased fecal volume P P P P 

4561 Daily observations, decreased fecal volume P P = present, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Daily Observations 

 Phase GES Animal Day 25 25 26 26 27 27 28 29 Number Observations Session AM PM AM PM AM PM AM AM Group 4 - 15000 ppm 4566 Miscellaneous, red exudate source unknown P 

4569 Daily observations, decreased fecal volume P P P 

P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 1 - 0 ppm 1551 Within normal limits P P P P P P P P P P 

1552 Within normal limits P P P P P P P P P P 1553 Within normal limits P P P P P P P P P P 1554 Within normal limits P P P P P P P P P P 1555 Within normal limits P P P P P P P P P P 1556 Within normal limits P P P P P P P P P P 1557 Within normal limits P P P P P P P P P P P 

1558 Within normal limits P P P P P P P P P P P 

1559 Within normal limits P P P P P P P P P P P 

1560 Within normal limits P P P P P P P P P P P 1561 Within normal limits P P P P P P P P P P P P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 1 - 0 ppm 1562 Within normal limits P P P P P P P P P P 

1563 Within normal limits P P P P P P P P P 

Dermal general, scab(s), ear(s), right P 

1564 Within normal limits P P P P P P P P P P 

1565 Within normal limits P P P P P P P P P P 1566 Within normal limits P P P P P P P P P P 1567 Within normal limits P P P P P P P P P P 1568 Within normal limits P P P P P P P P P P P 

1569 Within normal limits P P P P P P P P P P P 1570 Within normal limits P P P P P P P P P P P 1571 Within normal limits P P P P P P P P P P P P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 1 - 0 ppm 1572 Within normal limits P P P P P P P P P P P 

Group 2 - 2500 ppm 

2551 Within normal limits P P P P P P P P P P 2552 Within normal limits P P P P P P P P P P 2553 Within normal limits P P P P P P P P P P 2554 Within normal limits P P P P P P P P P P 2555 Within normal limits P P P P P P P P P 

Dermal general, alopecia (head/neck) 

2556 np Within normal limits P P P P P P P P P P 

2557 Within normal limits P P P P P P P P P P P 

2558 Within normal limits P P P P P P P P P P P np = not pregnant, P = present, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 2 - 2500 ppm 2559 Within normal limits P P P P P P P P P P P 

2560 Within normal limits P P P P P P P P P P P 2561 Within normal limits P P P P P P P P P P P 2562 Within normal limits P P P P P P P P P P 

2563 Within normal limits P P P P P P P P P P 2564 Within normal limits P P P P P 

Dermal general, abrasion (torso), vaginal area P P P P P 

2565 Within normal limits P P P P P P P P P P 

2566 Within normal limits P P P P P P P P P P 2567 Within normal limits P P P P P P P P P P 2568 Within normal limits P P P P P P P P P P P 

P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 2 - 2500 ppm 2569 Within normal limits P P P P P P P P P P P 

2570 Within normal limits P P P P P P P P P P P 2571 Within normal limits P P P P P P P P P P P 2572 Within normal limits P P P P P P P P P 

Dermal general, ano-genital staining, black 2 2 Dermal general, stains on fur (torso), ventral, black 1 1 

Group 3 - 10000 ppm 

3551 Within normal limits P P P P P P P P P P 

3552 Within normal limits P P P P P P P P P P 3553 Within normal limits P P P P P P P P P P 3554 Within normal limits P P P P P P P P P P P = present, 1 = slight, 2 = moderate 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 3 - 10000 ppm 3555 Within normal limits P P P P P P P 

Dermal general, abrasion (head/neck), ear(s), right P P P 

3556 Within normal limits P P P P P P P P P P P 

3557 Within normal limits P P P P P P P 

Dermal general, scab(s), ear(s), right P P P P 

3558 Within normal limits P P P P P P P P P P P 

3559 Within normal limits P P P P P P P P 

Dermal general, abrasion (head/neck), ear(s), bilateral P P P 

3560 Within normal limits P P P P P P P P P P P 

3561 Within normal limits P P P P P P P P P P P 3562 Within normal limits P P P P P P P P P 

Dermal general, alopecia (head/neck) 2 

P = present, 2 = moderate 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 3 - 10000 ppm 3563 Within normal limits P P P P P P P 

Dermal general, scab(s), ear(s), left P P P 

3564 np Within normal limits P P P P P P P P P P 

3565 Within normal limits P P P P P P P P P P 3566 Within normal limits P P P P P P P P P P 3567 Within normal limits P P P P P P P P 

Dermal general, abrasion (head/neck), ear(s), right P P P 

3568 np Within normal limits P P P P P P P P P P P 

3569 Within normal limits P P P P P P P P P P P 3570 Within normal limits P P P P P P P P P P P 3571 Within normal limits P P P P P P P P P P P np = not pregnant, P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 3 - 10000 ppm 3572 Within normal limits P P P P P P P P P P P 

Group 4 - 15000 ppm 

4551 Within normal limits P P P P P P P P P P 

General appearance, red exudate (limbs/tail), tail Dermal general, ulceration (limbs/tail), tail 

4552 Within normal limits P P P P P P P P P P 

4553 Within normal limits P P P P P P P P P P 4554 Within normal limits P P P P P P P P P P 4555 Within normal limits P P P P P P P P P P 4556 Within normal limits P P P P P P P P P P P 

P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 4 - 15000 ppm 4557 Within normal limits P 

General appearance, ear part missing unilateral P P P P P P P P P P Dermal general, alopecia (head/neck) 2 2 Dermal general, scab(s), ear(s) P Dermal general, scab(s), ear(s), bilateral P P P P P P P P 

4558 Within normal limits P P P P P P P P P P P 

4559 Within normal limits P P P P P P P P P P P 4560 Within normal limits P 

Dermal general, abrasion (head/neck), ear(s), left P P Dermal general, abrasion (head/neck), ear(s), bilateral P P P P P P P P 

4561 Within normal limits P P P P P P P P P P P 

4562 Within normal limits P P P P P P P P P P 

4563 Within normal limits P P P P P P P P P P P = present, 2 = moderate 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 4 - 15000 ppm 4564 Within normal limits P P P P P P 

Dermal general, abrasion (head/neck), ear(s), left P P P P 

4565 Within normal limits P 

Dermal general, scab(s), ear(s), bilateral P P P P P P Dermal general, scab(s), ear(s), unilateral P P P 

4566 Within normal limits P P P P P P P P P P 

4567 Within normal limits P P P P P P P P P P P 

4568 Within normal limits P P P P P P P 

Dermal general, scab(s), ear(s), bilateral P P P P Dermal general, scab(s), ear(s), unilateral, right 

4569 Within normal limits P P P P P P P P P P P 

4570 Within normal limits P P P P P P P P P P P 

Dermal general, alopecia (torso) 

P = present, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 1 2 3 4 5 6 7 8 9 10 1Group 4 - 15000 ppm 4571 Within normal limits P P P P P P P P P P P 

4572 Within normal limits P P P P P P P P P P P 

Dermal general, alopecia (head/neck) 

P = present, 2 = moderate 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 1 - 0 ppm 1551 Within normal limits P P P P P P P P P P P 

1552 Within normal limits P P P P P P P P P P P 1553 Within normal limits P P P P P P P P P P P 1554 Within normal limits P P P P P P P P P P P 1555 Within normal limits P P P P P P P P P P P 1556 Within normal limits P P P P P P P P P P P 1557 Within normal limits P P P P P P P P P P P 1558 Within normal limits P P P P P P P P P P P 1559 Within normal limits P P P P P P P P P P P 1560 Within normal limits P P P P P P P P P P P 1561 Within normal limits P P P P P P P P P P P P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 1 - 0 ppm 1562 Within normal limits P P P P P P P P P P P 

1563 Within normal limits P P P P P P P P P P P 1564 Within normal limits P P P P P P P P P P P 1565 Within normal limits P P P P P P P P P P P 

Dermal general, alopecia (limbs) Dermal general, alopecia (torso) 

1566 Within normal limits P P P P P P P P P P P 

1567 Within normal limits P P P P P P P P P P P 

Dermal general, alopecia (limbs) 

1568 Within normal limits P P P P P P P P P P P 

1569 Within normal limits P P P P P P P P P P P 1570 Within normal limits P P P P P P P P P P P P = present, 2 = moderate, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 1 - 0 ppm 1571 Within normal limits P P P P P P P P P P P 

1572 Within normal limits P P P P P P P P P P P Group 2 - 2500 ppm 

2551 Within normal limits P P P P P P P P P P P 

2552 Within normal limits P P P P P P P P P P P 2553 Within normal limits P P P P P P P P P P P 2554 Within normal limits P P P P P P P P P P P 2555 Dermal general, alopecia (limbs) 

Dermal general, alopecia (head/neck) 3 3 3 2 2 2 2 2 3 3 

2556 np Within normal limits P P P P P P P P P P P 

2557 Within normal limits P P P P P P P P P P P np = not pregnant, P = present, 2 = moderate, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 2 - 2500 ppm 2558 Within normal limits P P P P P P P P P P P 

2559 Within normal limits P P P P P P P P P P P 2560 Within normal limits P P P P P P P P P P P 2561 Within normal limits P P P P P P P P P P P 2562 Within normal limits P P P P P P P P P P P 2563 Within normal limits P P P P P P P P P P P 2564 Within normal limits P P P P P P P P P P P 2565 Within normal limits P P P P P P P P P P P 2566 Within normal limits P P P P P P P 

Dermal general, alopecia (torso) 2 2 2 2 

2567 Within normal limits P P P P P P P P P P P 

P = present, 2 = moderate, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 2 - 2500 ppm 2568 Within normal limits P P P P P P P P P P P 

2569 Within normal limits P P P P P P P P P P P 2570 Within normal limits P P P P P P P P P P P 2571 Within normal limits P P P P P P P P P P P 2572 Within normal limits P P P P P P P P P P P Group 3 - 10000 ppm 

3551 Within normal limits P P P P P P P P P P P 

3552 Within normal limits P P P P P P P P P P P 3553 Within normal limits P P P P P P P P P P P 3554 Within normal limits P P P P P P P P P P P 3555 Within normal limits P P P P P P P P P P P P = present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 3 - 10000 ppm 3556 Within normal limits P P P P P P P P P P P 

3557 Within normal limits P P P P P P P P P P P 3558 Within normal limits P P P P P P P P P P P 3559 Within normal limits P P P P P P P P P P 

Dermal general, scab(s), ear(s), right P 

3560 Within normal limits P P P P P P P P P P P 

3561 Within normal limits P P P P P P P P P P P 

3562 Dermal general, alopecia (head/neck) 2 2 2 2 2 2 2 2 2 2 2 

3563 Within normal limits P P P P P P P P P P P 

3564 np Within normal limits P P P P P P P P P P P 3565 Within normal limits P P P P P P P P P P P np = not pregnant, P = present, 2 = moderate 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 3 - 10000 ppm 3566 Within normal limits P P P P P P P P P P P 

3567 Within normal limits P P P P P P P P P P P 3568 np Within normal limits P P P P P P P P P P P 3569 Within normal limits P P P P P P P P P P P 

Dermal general, alopecia (limbs) 

3570 Within normal limits P P P P P P P P P P P 

3571 Within normal limits P P P P P P P P P P P 3572 Within normal limits P P P P P P P P P P P Group 4 - 15000 ppm 

4551 General appearance, red exudate (limbs/tail), tail P P 

Dermal general, ulceration (limbs/tail), tail P P P P P P P Dermal general, scab(s), tail P P P P 

np = not pregnant, P = present, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 4 - 15000 ppm 4552 Within normal limits P P P P P P P P P P P 

4553 Within normal limits P P P P P P P P P P P 4554 Within normal limits P P P P P P P P P P P 4555 Within normal limits P P P P P P P 

Dermal general, alopecia (head/neck) 2 2 2 2 Dermal general, alopecia (head/neck), snout 

4556 Within normal limits P P P P P P P P P P P 

4557 General appearance, ear part missing unilateral P P P P P P P P P P P 

Dermal general, alopecia (head/neck) 2 2 2 2 

4558 Within normal limits P P P P P P P P P P P 

4559 Within normal limits P P P P P P P P P P P 4560 Within normal limits P P P P P P P P P P P P = present, 2 = moderate 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 4 - 15000 ppm 4561 Within normal limits P P P P P P P P P P P 

4562 Within normal limits P P P P P P P P P P P 4563 Within normal limits P P P P P P P P P P P 

Dermal general, ano-genital staining, yellow 

4564 Within normal limits P P P P P P P P P P P 

4565 Within normal limits P P P P P P P P P P P 4566 Within normal limits P P P P P P P P P P P 

Miscellaneous, red exudate source unknown 

4567 Within normal limits P P P P P P P P P P P 

4568 Within normal limits 

Dermal general, scab(s), ear(s), unilateral, right P P P P P P P P P P P 

P = present, 1 = slight 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Females Individual Physical Examination Findings 

Animal Phase GES Number Observations Day 16 17 18 19 20 21 22 23 24 25 2Group 4 - 15000 ppm 4569 Within normal limits P P P P P P P P P P P 

General appearance, red exudate ano-genital area 

4570 Dermal general, alopecia (torso) 3 3 3 3 3 3 3 3 3 3 

4571 Within normal limits P P P P P P P P P P P 

4572 Within normal limits P 

Dermal general, alopecia (head/neck) 2 2 2 2 2 2 2 2 2 2 

P = present, 2 = moderate, 3 = extreme 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 1 - 0 ppm 1551 3597.5 3702.4 3750.7 3804.3 3838.3 3871.7 3942.8 3956.2 4027.3 4050.9 1552 3822.6 3880.1 3882.5 3844.1 3879.2 3935.4 3963.1 4047.5 4074.3 4136.5 1553 3163.6 3157.6 3153.3 3184.0 3131.7 3166.2 3238.2 3235.4 3261.6 3336.8 1554 3113.4 3159.5 3179.3 3186.8 3293.6 3333.1 3357.5 3429.9 3502.2 3569.7 1555 3236.0 3362.3 3381.7 3435.2 3542.6 3564.9 3589.5 3670.1 3698.9 3800.0 1556 3133.9 3170.5 3171.7 3121.3 3211.6 3258.5 3307.3 3330.1 3375.1 3356.4 1557 3194.1 3141.4 3075.1 3088.6 3160.1 3136.4 3153.4 3175.1 3192.1 3216.5 1558 3384.7 3459.5 3498.9 3523.5 3645.2 3653.0 3688.7 3708.9 3781.8 3827.5 1559 3349.1 3338.8 3312.1 3334.6 3375.5 3385.9 3428.6 3427.8 3464.2 3484.9 1560 3500.7 3564.6 3544.1 3551.2 3582.5 3657.5 3643.2 3656.2 3663.4 3700.7 1561 3835.1 3859.1 3836.3 3962.9 3938.1 3935.2 4020.1 4066.8 4093.3 4091.0 1562 3018.6 3067.4 2923.7 2956.6 2988.9 3101.8 3130.5 3221.2 3216.5 3264.8 1563 3368.4 3413.8 3502.9 3576.4 3667.2 3688.3 3709.8 3825.1 3892.0 3914.4 1564 3840.7 3875.5 3979.2 3994.5 4132.9 3780.3 4092.7 4187.1 4204.3 4259.6 1565 3327.8 3429.9 3534.5 3604.7 3696.7 3766.1 3795.1 3897.8 3923.0 3944.0 1566 3135.1 3192.0 3201.7 3240.6 3350.9 3356.7 3388.7 3464.3 3548.2 3626.3 1567 3276.0 3317.1 3329.2 3386.2 3439.5 3485.1 3429.1 3526.6 3701.6 3692.6 1568 3176.4 3282.4 3287.5 3329.8 3445.9 3500.8 3587.3 3617.3 3667.4 3645.9 1569 3116.7 3163.1 3129.1 3172.2 3237.1 3279.7 3238.9 3334.7 3413.5 3474.9 1570 2948.6 3119.6 3065.9 3015.4 3096.2 3015.0 3106.9 3160.3 3244.9 3310.0 1571 3566.9 3690.1 3729.5 3727.7 3861.0 3904.2 3898.2 3970.4 3951.6 4078.5 1572 3104.2 3242.6 3281.7 3375.0 3482.5 3504.7 3585.8 3586.9 3586.4 3678.4 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 1 - 0 ppm Mean 3327.7 3390.4 3397.8 3428.0 3499.9 3512.8 3558.9 3613.4 3658.3 3702.7 SD 264.03 262.60 293.38 303.32 308.02 286.20 304.93 313.10 307.56 308.81 N 22 22 22 22 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 2 - 2500 ppm 2551 3512.9 3457.3 3530.3 3570.3 3631.4 3674.7 3690.9 3740.3 3751.4 3764.8 2552 3158.1 3194.1 3218.7 3216.1 3346.8 3376.3 3353.7 3437.8 3453.6 3492.0 2553 3482.2 3570.4 3506.8 3490.5 3483.8 3509.8 3564.3 3616.3 3599.1 3585.5 2554 3344.0 3347.6 3274.6 3231.2 3256.0 3229.8 3233.3 3260.6 3284.1 3288.1 2555 3748.8 3845.1 3845.4 3884.1 3888.0 3916.0 3915.6 3908.2 3890.8 3907.7 2556 np 3366.6x 3352.7x 3325.0x 3363.8x 3354.1x 3376.8x 3379.1x 3392.9x 3392.1x 3403.9x 2557 3773.5 3760.0 3706.2 3700.2 3763.4 3708.5 3717.9 3709.7 3745.8 3700.1 2558 3040.2 3006.5 2994.5 2996.5 3077.4 3043.3 3056.8 3081.5 3129.8 3168.1 2559 3605.1 3693.5 3718.8 3702.5 3772.5 3793.9 3850.2 3847.4 3873.9 3881.4 2560 3352.4 3348.7 3368.0 3404.3 3471.0 3532.9 3545.7 3607.7 3658.3 3726.2 2561 3171.0 3152.8 3135.3 3147.8 3283.7 3280.0 3311.8 3363.9 3403.0 3517.1 2562 3174.8 3207.9 3275.4 3329.8 3392.9 3464.1 3518.8 3587.1 3606.9 3687.8 2563 3367.2 3462.2 3490.3 3526.6 3512.7 3357.9 3477.2 3474.7 3501.9 3517.0 2564 3177.8 3274.4 3278.7 3350.2 3351.9 3413.9 3457.1 3534.3 3589.4 3632.4 2565 3623.4 3730.3 3703.9 3733.8 3738.9 3855.2 3908.4 3929.7 3966.0 3995.7 2566 3460.6 3590.5 3626.1 3576.6 3690.8 3673.9 3739.1 3806.6 3836.6 3892.7 2567 3295.0 3374.9 3425.6 3465.0 3521.2 3592.4 3627.3 3702.2 3771.6 3797.9 2568 3536.2 3619.9 3612.7 3673.9 3782.1 3812.3 3830.2 3933.4 4066.4 4113.0 2569 3212.8 3235.3 3258.7 3360.8 3427.5 3509.5 3549.3 3331.8 3437.4 3556.7 2570 3202.9 3324.2 3443.1 3501.3 3527.2 3557.0 3603.3 3629.8 3640.2 3647.8 2571 3341.6 3505.1 3524.1 3649.2 3722.4 3731.8 3738.3 3919.7 3906.4 3841.0 2572 3210.1 3366.3 3420.6 3477.6 3610.0 3632.1 3638.8 3713.6 3795.0 3846.6 np = not pregnant, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 2 - 2500 ppm Mean 3371.0 3431.8 3445.6 3475.6 3535.8 3555.5 3587.0 3625.5 3662.3 3693.3 SD 205.98 221.89 215.41 217.60 208.41 221.49 222.69 236.83 235.96 226.93 N 21 21 21 21 21 21 21 21 21 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 3 - 10000 ppm 3551 3045.6 3038.4 3011.7 3042.6 3063.5 3067.0 3090.7 3205.7 3231.3 3283.2 3552 3603.4 3617.7 3659.4 3655.7 3742.6 3767.9 3787.2 3828.6 3934.4 3979.0 3553 3592.8 3632.4 3606.3 3609.1 3732.6 3744.7 3764.0 3873.0 3917.8 3918.2 3554 3456.7 3454.6 3437.5 3459.6 3494.4 3537.7 3568.7 3624.8 3601.9 3499.5 3555 3031.4 3098.2 3119.2 3069.5 3023.3 3069.1 3045.9 3056.0 3100.7 3073.4 3556 3092.7 3093.9 3084.2 3141.2 3187.3 3212.6 3218.2 3285.1 3266.2 3190.6 3557 3182.3 3203.6 3174.8 3188.8 3276.3 3304.3 3307.5 3370.8 3445.2 3486.8 3558 3575.6 3620.1 3600.3 3630.6 3714.5 3735.1 3821.8 3881.6 3931.4 3982.8 3559 3680.9 3646.3 3616.1 3608.4 3733.5 3801.7 3799.5 3890.5 3881.1 3918.1 3560 3667.4 3676.5 3608.2 3547.8 3534.1 3516.4 3554.2 3615.4 3666.0 3722.8 3561 3400.1 3460.6 3336.4 3278.2 3168.9 3146.2 3233.4 3330.9 3358.2 3399.9 3562 3194.2 3318.1 3366.6 3406.7 3553.5 3581.5 3616.0 3704.0 3783.0 3834.6 3563 3132.5 3228.3 3218.4 3319.7 3404.3 3475.1 3504.4 3602.2 3658.8 3753.8 3564 np 3264.4x 3233.4x 3269.5x 3325.0x 3318.8x 3388.6x 3397.1x 3426.0x 3455.2x 3463.5x 3565 3483.7 3528.9 3542.0 3574.7 3642.1 3688.5 3737.1 3740.4 3776.0 3792.1 3566 3810.7 3832.1 3806.9 3813.5 3742.2 3761.1 3728.6 3743.6 3635.6 3545.9 3567 3105.4 3178.2 3182.4 3163.9 3280.1 3327.8 3397.5 3440.8 3468.2 3534.8 3568 np 3051.0x 3162.2x 3171.2x 3282.5x 3250.6x 3368.5x 3371.1x 3347.4x 3326.6x 3432.4x 3569 2836.2 2862.3 2857.6 2755.3 2946.9 3035.0 3046.1 3079.7 3181.2 3206.4 3570 4011.7 4068.2 4018.1 4013.9 3898.8 3735.1 3768.5 3815.1 3920.1 3978.3 3571 3422.6 3466.5 3448.2 3467.0 3514.0 3570.3 3558.6 3614.2 3587.7 3619.1 3572 3023.4 3182.1 3285.3 3255.9 3419.3 3464.6 3458.8 3516.8 3509.2 3572.2 np = not pregnant, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 3 - 10000 ppm Mean 3367.5 3410.4 3399.0 3400.1 3453.6 3477.1 3500.3 3561.0 3592.7 3614.6 SD 312.54 301.03 288.77 297.00 276.99 262.25 264.58 265.27 267.23 284.11 N 20 20 20 20 20 20 20 20 20 20 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 4 - 15000 ppm 4551 3753.4 3757.4 3724.6 3762.2 3819.5 3771.2 3805.6 3799.4 3840.8 3912.1 4552 3058.7 3180.9 3130.2 3188.1 3320.5 3312.8 3334.2 3392.4 3499.9 3616.1 4553 3223.1 3258.7 3233.4 3245.3 3332.4 3345.7 3402.1 3446.3 3558.0 3591.3 4554 3156.1 3263.1 3246.0 3283.2 3309.6 3340.9 3300.6 3408.1 3485.4 3476.1 4555 3782.9 3783.1 3767.6 3789.3 3857.5 3910.4 3917.3 3974.9 4036.6 4105.4 4556 3196.8 3215.2 3222.3 3216.1 3303.6 3329.4 3329.7 3311.7 3268.8 3232.1 4557 3442.7 3489.8 3441.3 3526.1 3577.6 3656.0 3678.7 3745.3 3622.0 3566.0 4558 3308.5 3283.3 3279.7 3295.5 3425.0 3404.1 3381.7 3418.3 3482.3 3550.3 4559 3588.5 3620.5 3572.3 3622.8 3706.2 3680.2 3706.2 3793.8 3879.3 4012.4 4560 3455.3 3491.2 3499.8 3542.8 3600.1 3667.1 3577.2 3680.5 3653.8 3769.4 4561 3483.9 3505.3 3451.6 3419.8 3541.4 3557.8 3547.5 3534.9 3574.6 3697.4 4562 3083.9 3118.6 3204.9 3228.1 3334.7 3351.8 3410.1 3408.8 3570.9 3554.5 4563 3311.9 3425.8 3442.0 3419.3 3497.6 3552.3 3589.3 3654.0 3656.9 3699.8 4564 3337.5 3462.4 3430.6 3427.9 3553.9 3586.6 3656.5 3665.0 3733.5 3853.2 4565 3616.3 3670.4 3615.9 3588.7 3601.7 3595.3 3618.2 3613.6 3693.7 3744.3 4566 3670.2 3844.9 3832.3 3861.2 4025.6 4037.9 4048.6 4119.3 4177.2 3994.3 4567 3521.0 3580.8 3434.2 3398.9 3349.8 3365.8 3375.7 3426.9 3517.9 3614.9 4568 3796.2 3955.2 3962.3 3926.1 4139.3 4107.3 4116.0 4184.1 4250.5 4316.8 4569 3710.7 3748.6 3688.0 3692.2 3615.5 3672.9 3621.1 3672.1 3682.4 3138.3 4570 3535.6 3661.8 3711.1 3754.3 3814.2 3829.6 3819.2 3846.3 3878.6 3980.4 4571 3623.5 3671.1 3777.9 3820.4 3938.5 3924.8 3971.0 4031.3 4074.7 4161.7 4572 3509.9 3594.6 3630.5 3677.3 3713.5 3775.2 3847.5 3874.2 3885.7 3860.7 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weights 

Females (grams) 

Animal Phase GES Number Day 4 6 9 12 15 18 20 23 26 29 Group 4 - 15000 ppm Mean 3462.1 3526.5 3513.6 3531.2 3608.1 3626.1 3638.8 3681.9 3728.3 3747.6 SD 225.83 232.00 232.82 232.80 246.11 240.50 244.52 252.01 248.98 288.19 N 22 22 22 22 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 1 - 0 ppm 1551 104.9 48.3 53.6 34.0 33.4 71.1 13.4 71.1 23.6 240.4 1552 57.5 2.4 -38.4 35.1 56.2 27.7 84.4 26.8 62.2 83.0 1553 -6.0 -4.3 30.7 -52.3 34.5 72.0 -2.8 26.2 75.2 80.6 1554 46.1 19.8 7.5 106.8 39.5 24.4 72.4 72.3 67.5 198.0 1555 126.3 19.4 53.5 107.4 22.3 24.6 80.6 28.8 101.1 227.2 1556 36.6 1.2 -50.4 90.3 46.9 48.8 22.8 45.0 -18.7 136.8 1557 -52.7 -66.3 13.5 71.5 -23.7 17.0 21.7 17.0 24.4 12.0 1558 74.8 39.4 24.6 121.7 7.8 35.7 20.2 72.9 45.7 229.2 1559 -10.3 -26.7 22.5 40.9 10.4 42.7 -0.8 36.4 20.7 89.8 1560 63.9 -20.5 7.1 31.3 75.0 -14.3 13.0 7.2 37.3 78.6 1561 24.0 -22.8 126.6 -24.8 -2.9 84.9 46.7 26.5 -2.3 161.0 1562 48.8 -143.7 32.9 32.3 112.9 28.7 90.7 -4.7 48.3 63.1 1563 45.4 89.1 73.5 90.8 21.1 21.5 115.3 66.9 22.4 296.0 1564 34.8 103.7 15.3 138.4 -352.6 312.4 94.4 17.2 55.3 217.2 1565 102.1 104.6 70.2 92.0 69.4 29.0 102.7 25.2 21.0 365.2 1566 56.9 9.7 38.9 110.3 5.8 32.0 75.6 83.9 78.1 196.7 1567 41.1 12.1 57.0 53.3 45.6 -56.0 97.5 175.0 -9.0 112.0 1568 106.0 5.1 42.3 116.1 54.9 86.5 30.0 50.1 -21.5 304.9 1569 46.4 -34.0 43.1 64.9 42.6 -40.8 95.8 78.8 61.4 75.8 1570 171.0 -53.7 -50.5 80.8 -81.2 91.9 53.4 84.6 65.1 -12.7 1571 123.2 39.4 -1.8 133.3 43.2 -6.0 72.2 -18.8 126.9 208.1 1572 138.4 39.1 93.3 107.5 22.2 81.1 1.1 -0.5 92.0 343.2 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 1 - 0 ppm Mean 62.7 7.3 30.2 71.9 12.9 46.1 54.6 44.9 44.4 168.5 SD 52.74 56.58 43.28 49.36 90.15 71.27 38.83 42.08 38.98 105.24 N 22 22 22 22 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 2 - 2500 ppm 2551 -55.6 73.0 40.0 61.1 43.3 16.2 49.4 11.1 13.4 233.6 2552 36.0 24.6 -2.6 130.7 29.5 -22.6 84.1 15.8 38.4 159.6 2553 88.2 -63.6 -16.3 -6.7 26.0 54.5 52.0 -17.2 -13.6 -6.1 2554 3.6 -73.0 -43.4 24.8 -26.2 3.5 27.3 23.5 4.0 -114.3 2555 96.3 0.3 38.7 3.9 28.0 -0.4 -7.4 -17.4 16.9 70.5 2556 np -13.9x -27.7x 38.8x -9.7x 22.7x 2.3x 13.8x -0.8x 11.8x 26.4x 2557 -13.5 -53.8 -6.0 63.2 -54.9 9.4 -8.2 36.1 -45.7 -42.1 2558 -33.7 -12.0 2.0 80.9 -34.1 13.5 24.7 48.3 38.3 50.3 2559 88.4 25.3 -16.3 70.0 21.4 56.3 -2.8 26.5 7.5 156.7 2560 -3.7 19.3 36.3 66.7 61.9 12.8 62.0 50.6 67.9 197.0 2561 -18.2 -17.5 12.5 135.9 -3.7 31.8 52.1 39.1 114.1 159.0 2562 33.1 67.5 54.4 63.1 71.2 54.7 68.3 19.8 80.9 310.9 2563 95.0 28.1 36.3 -13.9 -154.8 119.3 -2.5 27.2 15.1 15.0 2564 96.6 4.3 71.5 1.7 62.0 43.2 77.2 55.1 43.0 182.7 2565 106.9 -26.4 29.9 5.1 116.3 53.2 21.3 36.3 29.7 178.1 2566 129.9 35.6 -49.5 114.2 -16.9 65.2 67.5 30.0 56.1 148.6 2567 79.9 50.7 39.4 56.2 71.2 34.9 74.9 69.4 26.3 252.4 2568 83.7 -7.2 61.2 108.2 30.2 17.9 103.2 133.0 46.6 210.3 2569 22.5 23.4 102.1 66.7 82.0 39.8 -217.5 105.6 119.3 314.0 2570 121.3 118.9 58.2 25.9 29.8 46.3 26.5 10.4 7.6 279.1 2571 163.5 19.0 125.1 73.2 9.4 6.5 181.4 -13.3 -65.4 233.2 2572 156.2 54.3 57.0 132.4 22.1 6.7 74.8 81.4 51.6 272.5 np = not pregnant, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 2 - 2500 ppm Mean 60.8 13.8 30.0 60.2 19.7 31.6 38.5 36.7 31.0 155.3 SD 63.58 46.68 44.07 46.89 57.48 30.81 73.33 37.80 44.66 118.70 N 21 21 21 21 21 21 21 21 21 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 3 - 10000 ppm 3551 -7.2 -26.7 30.9 20.9 3.5 23.7 115.0 25.6 51.9 52.3 3552 14.3 41.7 -3.7 86.9 25.3 19.3 41.4 105.8 44.6 169.5 3553 39.6 -26.1 2.8 123.5 12.1 19.3 109.0 44.8 0.4 131.6 3554 -2.1 -17.1 22.1 34.8 43.3 31.0 56.1 -22.9 -102.4 114.1 3555 66.8 21.0 -49.7 -46.2 45.8 -23.2 10.1 44.7 -27.3 -52.3 3556 1.2 -9.7 57.0 46.1 25.3 5.6 66.9 -18.9 -75.6 124.3 3557 21.3 -28.8 14.0 87.5 28.0 3.2 63.3 74.4 41.6 103.9 3558 44.5 -19.8 30.3 83.9 20.6 86.7 59.8 49.8 51.4 201.7 3559 -34.6 -30.2 -7.7 125.1 68.2 -2.2 91.0 -9.4 37.0 153.2 3560 9.1 -68.3 -60.4 -13.7 -17.7 37.8 61.2 50.6 56.8 -122.3 3561 60.5 -124.2 -58.2 -109.3 -22.7 87.2 97.5 27.3 41.7 -227.2 3562 123.9 48.5 40.1 146.8 28.0 34.5 88.0 79.0 51.6 297.9 3563 95.8 -9.9 101.3 84.6 70.8 29.3 97.8 56.6 95.0 276.1 3564 np -31.0x 36.1x 55.5x -6.2x 69.8x 8.5x 28.9x 29.2x 8.3x 163.7x 3565 45.2 13.1 32.7 67.4 46.4 48.6 3.3 35.6 16.1 208.2 3566 21.4 -25.2 6.6 -71.3 18.9 -32.5 15.0 -108.0 -89.7 -103.5 3567 72.8 4.2 -18.5 116.2 47.7 69.7 43.3 27.4 66.6 219.3 3568 np 111.2x 9.0x 111.3x -31.9x 117.9x 2.6x -23.7x -20.8x 105.8x 208.9x 3569 26.1 -4.7 -102.3 191.6 88.1 11.1 33.6 101.5 25.2 183.8 3570 56.5 -50.1 -4.2 -115.1 -163.7 33.4 46.6 105.0 58.2 -299.7 3571 43.9 -18.3 18.8 47.0 56.3 -11.7 55.6 -26.5 31.4 92.1 3572 158.7 103.2 -29.4 163.4 45.3 -5.8 58.0 -7.6 63.0 276.7 np = not pregnant, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 3 - 10000 ppm Mean 42.9 -11.4 1.1 53.5 23.5 23.3 60.6 31.7 21.9 90.0 SD 46.00 46.11 46.11 87.25 52.03 32.71 31.89 52.92 54.37 167.86 N 20 20 20 20 20 20 20 20 20 20 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 4 - 15000 ppm 4551 4.0 -32.8 37.6 57.3 -48.3 34.4 -6.2 41.4 71.3 48.2 4552 122.2 -50.7 57.9 132.4 -7.7 21.4 58.2 107.5 116.2 153.3 4553 35.6 -25.3 11.9 87.1 13.3 56.4 44.2 111.7 33.3 143.4 4554 107.0 -17.1 37.2 26.4 31.3 -40.3 107.5 77.3 -9.3 37.5 4555 0.2 -15.5 21.7 68.2 52.9 6.9 57.6 61.7 68.8 134.2 4556 18.4 7.1 -6.2 87.5 25.8 0.3 -18.0 -42.9 -36.7 114.5 4557 47.1 -48.5 84.8 51.5 78.4 22.7 66.6 -123.3 -56.0 188.9 4558 -25.2 -3.6 15.8 129.5 -20.9 -22.4 36.6 64.0 68.0 98.4 4559 32.0 -48.2 50.5 83.4 -26.0 26.0 87.6 85.5 133.1 85.7 4560 35.9 8.6 43.0 57.3 67.0 -89.9 103.3 -26.7 115.6 86.0 4561 21.4 -53.7 -31.8 121.6 16.4 -10.3 -12.6 39.7 122.8 42.2 4562 34.7 86.3 23.2 106.6 17.1 58.3 -1.3 162.1 -16.4 291.5 4563 113.9 16.2 -22.7 78.3 54.7 37.0 64.7 2.9 42.9 163.5 4564 124.9 -31.8 -2.7 126.0 32.7 69.9 8.5 68.5 119.7 194.1 4565 54.1 -54.5 -27.2 13.0 -6.4 22.9 -4.6 80.1 50.6 -52.2 4566 174.7 -12.6 28.9 164.4 12.3 10.7 70.7 57.9 -182.9 203.7 4567 59.8 -146.6 -35.3 -49.1 16.0 9.9 51.2 91.0 97.0 -205.1 4568 159.0 7.1 -36.2 213.2 -32.0 8.7 68.1 66.4 66.3 160.8 4569 37.9 -60.6 4.2 -76.7 57.4 -51.8 51.0 10.3 -544.1 -127.5 4570 126.2 49.3 43.2 59.9 15.4 -10.4 27.1 32.3 101.8 157.4 4571 47.6 106.8 42.5 118.1 -13.7 46.2 60.3 43.4 87.0 299.9 4572 84.7 35.9 46.8 36.2 61.7 72.3 26.7 11.5 -25.0 252.9 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Body Weight Changes 

Females (grams) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 4 - 15000 ppm Mean 64.4 -12.9 17.6 76.9 18.1 12.7 43.1 46.5 19.3 112.3 SD 54.05 53.91 33.75 65.05 34.64 39.92 36.71 59.91 146.79 123.37 N 22 22 22 22 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 1 - 0 ppm 1551 166.2 154.3 158.3 149.4 163.8 171.4 163.3 152.9 132.3 158.6 1552 131.9 152.8 139.2 101.3 176.3 193.2 167.3 131.7 153.8 149.7 1553 102.5 101.0 111.6 61.1 49.4 100.6 78.6 57.9 70.7 83.6 1554 128.8 136.3 149.1 148.7 162.9 146.0 129.0 117.5 111.0 148.8 1555 174.0 185.2 183.3 148.0 180.3 197.3 184.6 136.9 136.5 177.5 1556 117.0 135.6 103.8 113.6 130.2 129.8 116.3 94.7 89.2 122.1 1557 82.9 75.8 90.3 69.3 107.5 89.5 114.3 113.7 111.8 86.3 1558 181.8 175.8 173.4 173.1 172.0 181.4 178.4 183.6 141.0 174.7 1559 102.8 100.5 107.4 87.4 116.2 116.4 96.5 93.4 99.9 104.8 1560 118.3 122.6 134.0 96.1 141.6 137.0 95.0 69.2 92.7 125.5 1561 201.8 192.7 190.0 169.2 151.2 185.3 184.6 166.2 139.5 177.1 1562 53.4 77.9 86.3 86.7 101.8 115.9 129.5 120.1 129.8 92.2 1563 191.3 184.2 174.7 167.3 188.4 182.7 154.5 129.3 128.6 179.3 1564 191.3 191.2 192.9 172.0 30.4 190.8 179.0 175.8 137.2 152.9 1565 195.4 192.7 186.6 174.4 187.6 189.1 185.0 145.0 155.4 185.9 1566 121.1 120.6 103.4 85.5 114.6 126.6 117.8 95.8 106.9 109.0 1567 188.6 183.4 183.8 185.5 188.9 195.9 197.7 167.3 113.8 186.9 1568 137.4 144.0 149.1 151.0 161.9 186.9 165.2 128.1 114.3 156.6 1569 151.6 124.0 129.9 110.4 111.7 128.6 110.5 107.9 124.8 120.4 1570 182.4 169.5 195.0 163.5 180.8 204.9 186.8 179.1 196.6 179.3 1571 190.9 164.0 155.2 153.0 155.4 176.6 165.4 139.1 140.5 159.7 1572 195.1 194.5 194.3 190.6 193.5 201.5 191.6 186.7 191.9 194.4 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 1 - 0 ppm Mean 150.3 149.0 149.6 134.4 143.9 161.2 149.6 131.5 128.1 146.6 SD 42.79 38.03 36.69 40.66 44.69 36.59 36.79 36.40 30.14 35.33 N 22 22 22 22 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 2 - 2500 ppm 2551 97.4 150.4 149.4 150.2 166.5 172.9 153.8 143.2 145.9 156.8 2552 129.2 126.6 125.9 123.7 125.4 117.4 126.0 121.6 140.3 124.3 2553 158.2 151.8 134.1 113.8 128.8 119.1 104.1 46.5 59.1 130.3 2554 109.0 78.6 47.8 39.7 66.4 78.2 76.2 79.1 66.5 61.0 2555 148.8 169.6 166.4 143.9 156.0 151.8 109.4 45.3 6.6 158.0 2556 np 104.7x 95.6x 110.9x 115.6x 119.5x 102.9x 113.5x 109.9x 120.9x 109.3x 2557 119.7 111.7 103.9 58.4 47.9 115.6 73.8 44.3 109.8 85.5 2558 95.8 105.5 103.9 90.9 123.8 137.0 112.0 110.0 109.8 110.4 2559 166.1 157.9 158.2 152.8 161.3 181.2 168.2 136.6 117.6 160.9 2560 123.3 137.7 130.1 134.7 164.0 159.1 139.7 114.8 133.4 144.1 2561 84.4 79.2 78.5 90.9 114.4 130.4 146.8 120.7 138.0 96.4 2562 154.1 144.1 165.2 169.8 177.5 183.0 148.3 126.1 125.4 166.8 2563 195.4 191.3 180.1 112.3 26.3 91.3 135.2 123.8 102.0 122.3 2564 155.9 157.6 150.5 120.2 142.6 178.1 174.2 147.1 122.5 147.8 2565 186.0 159.7 152.5 118.9 159.8 177.7 166.9 136.4 146.1 152.0 2566 168.4 164.3 149.7 136.1 147.7 176.4 163.8 128.9 144.6 153.3 2567 150.2 162.3 148.8 134.2 156.0 175.4 164.5 154.6 135.8 153.9 2568 176.2 163.8 166.4 155.0 191.5 194.4 194.9 184.9 151.2 172.8 2569 185.1 154.7 161.5 150.5 175.9 177.4 121.9 93.3 185.6 163.1 2570 201.4 194.7 187.1 129.8 182.2 200.2 173.4 110.8 111.3 177.3 2571 192.1 183.5 179.1 169.8 187.8 199.1 187.3 154.7 85.2 182.8 2572 194.8 193.6 194.7 192.2 198.5 197.2 188.5 185.1 188.3 195.1 np = not pregnant, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 2 - 2500 ppm Mean 152.0 149.5 144.5 128.0 142.9 157.7 144.2 119.4 120.2 143.6 SD 36.10 33.31 36.41 36.32 46.61 36.43 35.02 40.06 41.45 33.61 N 21 21 21 21 21 21 21 21 21 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 3 - 10000 ppm 3551 70.6 89.7 114.0 121.8 130.7 131.3 128.0 131.9 136.6 116.5 3552 152.6 148.5 138.0 116.7 133.1 141.7 148.0 139.0 146.9 135.2 3553 121.3 134.4 131.9 116.7 150.0 159.5 167.8 132.6 111.2 137.0 3554 141.3 110.7 109.1 107.9 132.1 135.9 108.4 39.7 1.5 116.9 3555 105.0 116.9 113.8 35.2 60.0 46.2 46.9 55.9 107.5 76.4 3556 161.3 158.4 161.6 122.4 136.9 152.6 135.7 113.2 126.0 145.9 3557 122.5 120.3 119.0 123.7 123.8 130.6 117.8 109.5 106.5 123.0 3558 140.7 127.6 131.4 112.6 145.5 161.5 165.4 151.7 158.0 133.9 3559 95.9 75.7 85.7 100.4 128.7 147.9 152.1 125.9 110.0 104.8 3560 120.5 100.2 80.1 33.9 44.6 83.5 115.6 108.6 112.7 67.4 3561 105.1 75.6 37.6 6.2 11.5 54.1 82.0 90.6 103.5 40.7 3562 188.9 189.4 188.3 185.9 193.1 191.3 195.7 191.2 192.3 189.5 3563 187.6 180.7 158.8 147.1 160.5 170.5 150.0 116.4 145.2 163.0 3564 np 124.2x 152.3x 168.6x 163.2x 167.3x 162.0x 162.2x 173.8x 170.1x 162.7x 3565 163.4 153.2 164.8 157.3 171.1 174.0 178.1 147.5 121.8 163.4 3566 173.4 144.9 136.7 74.3 61.2 96.2 49.6 17.5 24.2 103.1 3567 133.4 118.2 111.5 108.6 136.1 146.4 128.7 123.2 127.6 122.6 3568 np 167.1x 166.4x 174.5x 170.9x 175.6x 172.0x 160.0x 164.0x 161.8x 171.9x 3569 154.2 119.0 79.4 77.4 124.7 138.6 131.0 122.0 106.0 105.6 3570 142.2 131.5 106.3 39.5 10.0 24.0 38.0 60.0 83.1 65.0 3571 170.3 144.5 142.1 149.4 164.8 172.6 162.3 146.5 121.7 153.4 3572 199.1 189.7 175.8 187.0 191.0 190.3 190.4 166.3 176.4 186.5 np = not pregnant, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 3 - 10000 ppm Mean 142.4 131.5 124.3 106.2 120.5 132.4 129.6 114.5 115.9 122.5 SD 33.80 33.32 36.76 49.59 54.23 47.67 46.01 43.28 44.04 39.80 N 20 20 20 20 20 20 20 20 20 20 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 4 - 15000 ppm 4551 138.9 127.9 110.3 75.3 87.7 96.6 127.4 110.2 120.4 99.8 4552 123.3 118.9 124.0 116.6 146.7 156.4 131.0 181.3 168.1 130.8 4553 110.8 102.0 125.6 134.9 137.1 162.3 155.1 142.5 151.1 130.3 4554 136.5 153.0 144.4 134.8 147.8 178.9 135.7 108.4 77.1 149.8 4555 171.7 135.9 133.3 147.4 167.1 173.1 184.9 169.4 168.6 149.8 4556 133.4 132.7 138.9 125.8 145.8 158.2 127.8 51.5 74.5 139.0 4557 147.2 154.2 162.0 133.2 159.6 175.2 143.5 45.3 42.0 155.5 4558 93.4 87.1 84.1 84.2 101.3 111.7 104.0 108.3 121.7 92.4 4559 154.6 181.9 173.5 158.8 172.9 176.2 165.0 159.8 159.1 172.4 4560 182.3 166.0 161.6 157.4 149.3 118.4 131.4 152.0 147.8 152.9 4561 107.2 70.2 60.8 65.3 70.8 90.1 73.9 46.7 129.9 70.1 4562 195.4 168.8 180.8 191.3 195.7 195.4 198.4 182.9 161.3 185.8 4563 189.9 156.5 162.0 140.6 157.0 181.6 172.6 145.8 135.4 158.0 4564 168.8 140.9 133.2 133.8 143.6 177.8 169.6 161.7 176.7 143.6 4565 146.1 117.0 109.1 61.8 89.4 116.6 97.2 88.7 121.6 97.5 4566 196.1 173.7 184.1 182.4 191.5 183.4 191.1 142.8 88.5 183.0 4567 131.5 62.0 67.7 44.4 45.4 61.6 73.0 85.7 118.3 55.8 4568 193.2 159.7 161.4 162.2 172.9 191.8 178.6 150.3 151.7 168.0 4569 128.1 108.8 100.3 21.9 39.1 34.6 55.4 44.9 12.5 62.8 4570 201.0 179.7 182.7 168.3 181.3 195.5 184.6 183.8 193.5 180.5 4571 192.4 190.4 194.6 199.0 198.5 202.8 188.7 190.5 191.4 196.6 4572 183.9 166.1 179.1 142.2 183.6 190.7 149.8 101.8 93.9 171.0 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Food Consumption Values 

Females (grams/day) 

Animal Phase GES Number Day 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 Group 4 - 15000 ppm Mean 155.7 138.8 139.7 126.4 140.2 151.3 142.7 125.2 127.5 138.4 SD 33.15 36.22 38.78 48.22 47.47 47.45 41.55 48.65 47.16 41.56 N 22 22 22 22 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 138 

 MA-252E-93-23 Final Report

 Individual Animal Gross 

and Microscopic Observations 

Preface Appendix H 

Key to Abbreviations 

Oviducts/Fallop = Oviducts/Fallopian 

w/ = with 

Corresponding exposure levels for each group were as follows: 

Group 1 - 0 ppm 

Group 2 - 2500 ppm 

Group 3 - 10000 ppm 

Group 4 - 15000 ppm 

SANITIZED
 Huntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers 

 Mettlers Road Study number: 09-4348  P.O. Box 2360  Rabbit/New Zealand White Study start date: 08-Mar-10 Se ________________________________________________________________________________________________________________ Animal Group/ Date and time Date data Opr  number Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measure Severity levels  ---------------------------------------------------------------------------------------------------------------- 1551 F 1/1 06-Apr-10 09:32 06-Apr-10 358 Within Normal Limits  1552 F 1/1 06-Apr-10 09:27 06-Apr-10 322 Within Normal Limits  1553 F 1/1 06-Apr-10 09:59 06-Apr-10 322 Within Normal Limits  1554 F 1/1 06-Apr-10 10:06 06-Apr-10 250 Within Normal Limits  1555 F 1/1 06-Apr-10 10:41 06-Apr-10 358 Within Normal Limits  1556 F 1/1 06-Apr-10 11:23 06-Apr-10 362 Within Normal Limits  1557 F 1/1 07-Apr-10 09:12 07-Apr-10 362 Within Normal Limits  1558 F 1/1 07-Apr-10 09:13 07-Apr-10 322 Within Normal Limits  1559 F 1/1 07-Apr-10 09:43 07-Apr-10 358 Within Normal Limits  1560 F 1/1 07-Apr-10 09:52 07-Apr-10 322 Within Normal Limits  1561 F 1/1 07-Apr-10 10:45 07-Apr-10 362 Within Normal Limits  1562 F 1/1 08-Apr-10 09:15 08-Apr-10 362 Within Normal Limits  1563 F 1/1 Placenta  08-Apr-10 09:29 08-Apr-10 200 Discolored, , Tan, Areas, 0.2 - 0.5 cm, Moderate  #11  Tissues marked 'Within Normal Limits':  08-Apr-10 09:33 08-Apr-10 200 Body (entire)  1564 F 1/1 08-Apr-10 10:01 08-Apr-10 362 Within Normal Limits  1565 F 1/1 08-Apr-10 10:16 08-Apr-10 205 Within Normal Limits  1566 F 1/1 08-Apr-10 10:48 08-Apr-10 222 Within Normal Limits  1567 F 1/1 08-Apr-10 11:39 08-Apr-10 222 Within Normal Limits  1568 F 1/1 09-Apr-10 09:15 09-Apr-10 358 Within Normal Limits  1569 F 1/1 09-Apr-10 09:23 09-Apr-10 322 Within Normal Limits  1570 F 1/1 09-Apr-10 09:57 09-Apr-10 358 Within Normal Limits 

SANITIZED
 Huntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers 

 Mettlers Road Study number: 09-4348  P.O. Box 2360  Rabbit/New Zealand White Study start date: 08-Mar-10 Se ________________________________________________________________________________________________________________ Animal Group/ Date and time Date data Opr  number Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measure Severity levels  ---------------------------------------------------------------------------------------------------------------- 1571 F 1/1 09-Apr-10 10:37 09-Apr-10 362 Within Normal Limits  1572 F 1/1 09-Apr-10 11:16 09-Apr-10 362 Within Normal Limits  2551 F 2/1 06-Apr-10 09:41 06-Apr-10 362 Within Normal Limits  2552 F 2/1 06-Apr-10 09:25 06-Apr-10 250 Within Normal Limits  2553 F 2/1 06-Apr-10 10:03 06-Apr-10 358 Within Normal Limits  2554 F 2/1 06-Apr-10 10:34 06-Apr-10 222 Within Normal Limits  2555 F 2/1 06-Apr-10 10:41 06-Apr-10 46 Within Normal Limits  2556 F 2/1 Oviducts/Fallop  13-Apr-10 12:43 06-Apr-10 200 Cyst, Right, Clear, Fluid, 0.2 - 0.5 cm,  Tissues marked 'Within Normal Limits':  06-Apr-10 11:01 06-Apr-10 200 Body (entire) Placenta  2557 F 2/1 07-Apr-10 09:15 07-Apr-10 358 Within Normal Limits  2558 F 2/1 Oviducts/Fallop  07-Apr-10 09:14 07-Apr-10 205 Cyst, Left, Clear, Fluid, 0.2 - 0.5 cm,  Tissues marked 'Within Normal Limits':  07-Apr-10 09:18 07-Apr-10 205 Body (entire) Placenta  2559 F 2/1 07-Apr-10 09:46 07-Apr-10 200 Within Normal Limits  2560 F 2/1 07-Apr-10 10:01 07-Apr-10 205 Within Normal Limits  2561 F 2/1 07-Apr-10 10:52 07-Apr-10 222 Within Normal Limits  2562 F 2/1 08-Apr-10 09:29 08-Apr-10 222 Within Normal Limits  2563 F 2/1 08-Apr-10 09:28 08-Apr-10 205 Within Normal Limits  2564 F 2/1 08-Apr-10 09:58 08-Apr-10 358 Within Normal Limits  2565 F 2/1 08-Apr-10 10:09 08-Apr-10 322 Within Normal Limits  2566 F 2/1 08-Apr-10 10:48 08-Apr-10 362 Within Normal Limits  2567 F 2/1 08-Apr-10 11:30 08-Apr-10 362 Within Normal Limits 

SANITIZED
 Huntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers 

 Mettlers Road Study number: 09-4348  P.O. Box 2360  Rabbit/New Zealand White Study start date: 08-Mar-10 Se ________________________________________________________________________________________________________________ Animal Group/ Date and time Date data Opr  number Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measure Severity levels  ---------------------------------------------------------------------------------------------------------------- 2568 F 2/1 09-Apr-10 09:24 09-Apr-10 222 Within Normal Limits  2569 F 2/1 09-Apr-10 09:27 09-Apr-10 250 Within Normal Limits  2570 F 2/1 09-Apr-10 10:04 09-Apr-10 222 Within Normal Limits  2571 F 2/1 09-Apr-10 10:45 09-Apr-10 358 Within Normal Limits  2572 F 2/1 09-Apr-10 11:19 09-Apr-10 358 Within Normal Limits  3551 F 3/1 Placenta  06-Apr-10 09:43 06-Apr-10 222 Abnormal Contents, , Red, Gelatinous material, , Mode Number 2.  Tissues marked 'Within Normal Limits':  06-Apr-10 09:45 06-Apr-10 222 Body (entire)  3552 F 3/1 06-Apr-10 09:49 06-Apr-10 205 Within Normal Limits  3553 F 3/1 06-Apr-10 10:01 06-Apr-10 46 Within Normal Limits  3554 F 3/1 06-Apr-10 10:34 06-Apr-10 362 Within Normal Limits  3555 F 3/1 06-Apr-10 11:30 06-Apr-10 222 Within Normal Limits  3556 F 3/1 07-Apr-10 09:22 07-Apr-10 222 Within Normal Limits  3557 F 3/1 07-Apr-10 09:22 07-Apr-10 46 Within Normal Limits  3558 F 3/1 07-Apr-10 09:47 07-Apr-10 250 Within Normal Limits  3559 F 3/1 07-Apr-10 09:57 07-Apr-10 46 Within Normal Limits  3560 F 3/1 07-Apr-10 10:47 07-Apr-10 358 Within Normal Limits  3561 F 3/1 07-Apr-10 11:27 07-Apr-10 362 Within Normal Limits  3562 F 3/1 Uterus w/ Cervix  08-Apr-10 09:11 08-Apr-10 358 Absent, Right Horn, , , ,  Some rudimentary uterine tissue present.  Tissues marked 'Within Normal Limits':  08-Apr-10 09:24 08-Apr-10 358 Body (entire) Placenta  3563 F 3/1 08-Apr-10 09:34 08-Apr-10 250 Within Normal Limits 

SANITIZED
 Huntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers 

 Mettlers Road Study number: 09-4348  P.O. Box 2360  Rabbit/New Zealand White Study start date: 08-Mar-10 Se ________________________________________________________________________________________________________________ Animal Group/ Date and time Date data Opr  number Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measure Severity levels  ---------------------------------------------------------------------------------------------------------------- 3564 F 3/1 08-Apr-10 09:56 08-Apr-10 222 Within Normal Limits  3565 F 3/1 08-Apr-10 10:17 08-Apr-10 250 Within Normal Limits  3566 F 3/1 08-Apr-10 10:49 08-Apr-10 358 Within Normal Limits  3567 F 3/1 09-Apr-10 09:17 09-Apr-10 205 Within Normal Limits  3568 F 3/1 09-Apr-10 09:21 09-Apr-10 46 Within Normal Limits  3569 F 3/1 09-Apr-10 10:05 09-Apr-10 46 Within Normal Limits  3570 F 3/1 09-Apr-10 10:51 09-Apr-10 222 Within Normal Limits  3571 F 3/1 09-Apr-10 11:27 09-Apr-10 222 Within Normal Limits  3572 F 3/1 09-Apr-10 11:54 09-Apr-10 362 Within Normal Limits  4551 F 4/1 06-Apr-10 09:31 06-Apr-10 200 Within Normal Limits  4552 F 4/1 06-Apr-10 09:36 06-Apr-10 46 Within Normal Limits  4553 F 4/1 06-Apr-10 10:01 06-Apr-10 200 Within Normal Limits  4554 F 4/1 06-Apr-10 10:33 06-Apr-10 205 Within Normal Limits  4555 F 4/1 06-Apr-10 11:24 06-Apr-10 205 Within Normal Limits  4556 F 4/1 07-Apr-10 09:11 07-Apr-10 200 Within Normal Limits  4557 F 4/1 07-Apr-10 09:57 07-Apr-10 362 Within Normal Limits  4558 F 4/1 07-Apr-10 10:02 07-Apr-10 222 Within Normal Limits  4559 F 4/1 07-Apr-10 10:20 07-Apr-10 358 Within Normal Limits  4560 F 4/1 07-Apr-10 10:51 07-Apr-10 205 Within Normal Limits  4561 F 4/1 07-Apr-10 11:34 07-Apr-10 222 Within Normal Limits  4562 F 4/1 08-Apr-10 09:20 08-Apr-10 322 Within Normal Limits 

SANITIZED
 Huntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers 

 Mettlers Road Study number: 09-4348  P.O. Box 2360  Rabbit/New Zealand White Study start date: 08-Mar-10 Se ________________________________________________________________________________________________________________ Animal Group/ Date and time Date data Opr  number Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measure Severity levels  ---------------------------------------------------------------------------------------------------------------- 4563 F 4/1 Placenta  08-Apr-10 09:34 08-Apr-10 46 Discolored, , Pale, , , Moderate  #5  Tissues marked 'Within Normal Limits':  08-Apr-10 09:38 08-Apr-10 46 Body (entire)  4564 F 4/1 08-Apr-10 10:06 08-Apr-10 200 Within Normal Limits  4565 F 4/1 08-Apr-10 10:21 08-Apr-10 46 Within Normal Limits  4566 F 4/1 Placenta  08-Apr-10 11:01 08-Apr-10 205 Discolored, , Red, , , Severe  Placenta 1  08-Apr-10 11:02 08-Apr-10 205 Enlarged, , , , , Severe  Placenta 1  08-Apr-10 11:07 08-Apr-10 205 Discolored, , Pale, , , Moderate  Placenta # 2, 6, 7, 8.  08-Apr-10 11:07 08-Apr-10 205 Small, , , , , Moderate  Placenta 6  Tissues marked 'Within Normal Limits':  08-Apr-10 11:11 08-Apr-10 205 Body (entire)  4567 F 4/1 09-Apr-10 09:20 09-Apr-10 200 Within Normal Limits  4568 F 4/1 09-Apr-10 10:00 09-Apr-10 362 Within Normal Limits  4569 F 4/1 Spleen  09-Apr-10 08:37 09-Apr-10 250 Small, , , , , Moderate  Tissues marked 'Within Normal Limits':  09-Apr-10 08:37 09-Apr-10 250 Body (entire) Placenta  4570 F 4/1 09-Apr-10 10:03 09-Apr-10 205 Within Normal Limits  4571 F 4/1 09-Apr-10 10:51 09-Apr-10 205 Within Normal Limits  4572 F 4/1 09-Apr-10 11:34 09-Apr-10 205 Within Normal Limits 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 1 - 0 ppm 1551 9 8 0 0 0 0 4 4 8 50 1552 9 9 0 0 0 0 3 6 9 33 1553 10 10 0 0 0 0 1 9 10 10 1554 8 8 0 0 0 0 4 4 8 50 1555 14 11 0 0 0 0 6 5 11 55 1556 10 10 0 0 0 0 4 6 10 40 1557 8 8 0 0 0 0 7 1 8 88 1558 7 7 0 0 0 0 2 5 7 29 1559 7 5 0 0 0 0 4 1 5 80 1560 10 8 0 0 0 0 5 3 8 63 1561 11 10 0 0 0 0 5 5 10 50 1562 7 7 2 0 2 0 2 3 5 40 1563 12 12 0 0 0 0 4 8 12 33 1564 12 12 1 1 2 0 5 5 10 50 1565 13 13 1 1 2 0 6 5 11 55 1566 12 12 1 0 1 0 5 6 11 45 1567 10 10 0 0 0 0 9 1 10 90 1568 9 9 0 0 0 0 4 5 9 44 1569 10 9 1 0 1 0 7 1 8 88 1570 11 10 2 0 2 0 3 5 8 38 1571 11 11 3 0 3 0 5 3 8 63 1572 10 9 0 0 0 0 3 6 9 33 Impl. = Implantations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 1 - 0 ppm Mean 10.0 9.5 0.5 0.1 0.6 0.0 4.5 4.4 8.9 51.1 SD 1.93 1.95 0.86 0.29 0.96 0.00 1.84 2.17 1.81 20.6

N 22 22 22 22 22 22 22 22 22 22 

Impl. = Implantations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 2 - 2500 ppm 2551 9 8 1 0 1 0 6 1 7 86 2552 4 4 0 0 0 0 3 1 4 75 2553 11 11 1 0 1 0 6 4 10 60 2554 9 8 0 0 0 0 5 3 8 63 2555 10 9 0 0 0 0 7 2 9 78 2556 np - - - - - - - - - - 2557 10 9 0 0 0 0 5 4 9 56 2558 8 5 0 0 0 0 1 4 5 20 2559 6 6 0 0 0 0 1 5 6 17 2560 9 9 0 0 0 0 4 5 9 44 2561 10 9 0 0 0 0 3 6 9 33 2562 12 12 1 0 1 0 6 5 11 55 2563 9 9 0 1 1 0 4 4 8 50 2564 9 9 0 0 0 0 3 6 9 33 2565 9 9 0 1 1 0 4 4 8 50 2566 10 10 0 0 0 0 8 2 10 80 2567 10 10 0 0 0 0 3 7 10 30 2568 10 8 0 0 0 0 4 4 8 50 2569 10 10 0 0 0 0 2 8 10 20 2570 9 9 0 0 0 0 4 5 9 44 2571 10 10 0 0 0 0 4 6 10 40 2572 10 10 0 0 0 0 4 6 10 40 Impl. = Implantations, np = not pregnant 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 2 - 2500 ppm Mean 9.2 8.8 0.1 0.1 0.2 0.0 4.1 4.4 8.5 48.7 SD 1.67 1.87 0.36 0.30 0.44 0.00 1.80 1.86 1.78 19.9

N 21 21 21 21 21 21 21 21 21 21 

Impl. = Implantations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 3 - 10000 ppm 3551 11 7 1 0 1 0 1 5 6 17 3552 14 13 1 0 1 0 9 3 12 75 3553 9 9 0 0 0 0 6 3 9 67 3554 9 9 0 0 0 0 4 5 9 44 3555 10 9 1 0 1 0 1 7 8 13 3556 9 9 0 0 0 0 1 8 9 11 3557 10 10 0 0 0 0 5 5 10 50 3558 7 7 0 0 0 0 4 3 7 57 3559 6 6 0 0 0 0 3 3 6 50 3560 12 8 0 0 0 0 4 4 8 50 3561 8 8 0 1 1 0 4 3 7 57 3562 11 7 0 0 0 0 5 2 7 71 3563 13 13 0 0 0 0 3 10 13 23 3564 np - - - - - - - - - - 3565 9 6 0 0 0 0 3 3 6 50 3566 9 8 0 0 0 0 7 1 8 88 3567 9 9 1 1 2 0 4 3 7 57 3568 np - - - - - - - - - - 3569 12 11 0 0 0 0 7 4 11 64 3570 13 10 1 0 1 0 3 6 9 33 3571 13 3 0 0 0 0 3 0 3 100 3572 9 9 0 2 2 0 4 3 7 57 Impl. = Implantations, np = not pregnant 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 3 - 10000 ppm Mean 10.2 8.6 0.3 0.2 0.5 0.0 4.1 4.1 8.1 51.7 SD 2.16 2.33 0.44 0.52 0.69 0.00 2.06 2.35 2.29 23.6

N 20 20 20 20 20 20 20 20 20 20 

Impl. = Implantations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 4 - 15000 ppm 4551 12 12 1 0 1 0 8 3 11 73 4552 9 8 0 0 0 0 4 4 8 50 4553 9 8 0 0 0 0 7 1 8 88 4554 9 9 0 0 0 0 8 1 9 89 4555 11 11 2 0 2 0 4 5 9 44 4556 8 7 1 0 1 0 3 3 6 50 4557 10 10 0 1 1 0 6 3 9 67 4558 9 9 0 0 0 0 3 6 9 33 4559 10 5 0 0 0 0 3 2 5 60 4560 11 9 0 0 0 0 3 6 9 33 4561 8 7 0 1 1 0 4 2 6 67 4562 10 9 0 0 0 0 5 4 9 56 4563 12 12 0 0 0 0 6 6 12 50 4564 11 11 1 0 1 0 7 3 10 70 4565 12 11 0 0 0 0 2 9 11 18 4566 11 10 0 0 0 5 3 2 5 60 4567 11 10 0 0 0 0 1 9 10 10 4568 11 11 0 0 0 0 5 6 11 45 4569 pd 10x 10x 0x 0x 0x 0x 3x 7x 10x 30x 4570 12 7 0 1 1 0 2 4 6 33 4571 10 8 0 0 0 0 3 5 8 38 4572 12 10 0 0 0 0 6 4 10 60 Impl. = Implantations, pd = premature delivery, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Cesarean Section Data 

Animal Corpora Implant- Resorptions Dead Live Fetuses Sex Rat

Number Lutea ations Early Late Total Fetuses Male Female Total (% Mal

Group 4 - 15000 ppm Mean 10.4 9.2 0.2 0.1 0.4 0.2 4.4 4.2 8.6 52.1 SD 1.32 1.84 0.54 0.36 0.59 1.09 2.04 2.25 2.04 20.3

N 21 21 21 21 21 21 21 21 21 21 

Impl. = Implantations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 1 - 0 ppm 

1551 3702 4051 349 487.10 3564 -139 1552 3880 4137 256 521.20 3615 -265 1553 3158 3337 179 563.00 2774 -384 1554 3160 3570 410 501.00 3069 -91 1555 3362 3800 438 610.50 3190 -173 1556 3171 3356 186 545.00 2811 -359 1557 3141 3217 75 420.70 2796 -346 1558 3460 3828 368 418.90 3409 -51 1559 3339 3485 146 285.00 3200 -139 1560 3565 3701 136 506.30 3194 -370 1561 3859 4091 232 578.20 3513 -346 1562 3067 3265 197 323.93 2941 -127 1563 3414 3914 501 567.60 3347 -67 1564 3876 4260 384 613.10 3647 -229 1565 3430 3944 514 560.40 3384 -46 1566 3192 3626 434 623.70 3003 -189 1567 3317 3693 376 492.50 3200 -117 1568 3282 3646 364 532.40 3114 -169 1569 3163 3475 312 461.40 3014 -150 1570 3120 3310 190 449.20 2861 -259 1571 3690 4079 388 512.70 3566 -124 1572 3243 3678 436 525.70 3153 -90 

Adj = Adjusted, Bwt = Bodyweight 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 1 - 0 ppm 

Mean 3390 3703 312 504.52 3198 -192 

SD 263 309 127 86.445 277 110 

N 22 22 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 2 - 2500 ppm 

2551 3457 3765 308 467.90 3297 -160 2552 3194 3492 298 269.00 3223 29 2553 3570 3586 15 537.30 3048 -522 2554 3348 3288 -60 450.20 2838 -510 2555 3845 3908 63 479.20 3429 -417 

2556 np - - - - - - 

2557 3760 3700 -60 434.90 3265 -495 2558 3007 3168 162 327.80 2840 -166 2559 3694 3881 188 396.40 3485 -209 2560 3349 3726 378 567.50 3159 -190 2561 3153 3517 364 520.40 2997 -156 2562 3208 3688 480 553.18 3135 -73 2563 3462 3517 55 441.10 3076 -386 2564 3274 3632 358 561.00 3071 -203 2565 3730 3996 265 446.90 3549 -181 2566 3591 3893 302 555.80 3337 -254 2567 3375 3798 423 553.20 3245 -130 2568 3620 4113 493 557.60 3555 -65 2569 3235 3557 321 469.10 3088 -148 2570 3324 3648 324 487.90 3160 -164 2571 3505 3841 336 595.90 3245 -260 2572 3366 3847 480 555.90 3291 -76 

Adj = Adjusted, Bwt = Bodyweight, np = not pregnant 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 2 - 2500 ppm 

Mean 3432 3693 262 487.06 3206 -226 

SD 222 227 172 83.754 200 154 

N 21 21 21 21 21 21 

Adj = Adjusted, Bwt = Bodyweight 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 3 - 10000 ppm 

3551 3038 3283 245 354.20 2929 -109 3552 3618 3979 361 593.00 3386 -232 3553 3632 3918 286 483.90 3434 -198 3554 3455 3500 45 419.30 3080 -374 3555 3098 3073 -25 439.00 2634 -464 3556 3094 3191 97 561.00 2630 -464 3557 3204 3487 283 581.50 2905 -298 3558 3620 3983 363 496.70 3486 -134 3559 3646 3918 272 414.00 3504 -142 3560 3677 3723 46 421.50 3301 -375 3561 3461 3400 -61 479.50 2920 -540 3562 3318 3835 517 410.03 3425 106 3563 3228 3754 526 655.70 3098 -130 

3564 np - - - - - - 

3565 3529 3792 263 452.60 3340 -189 3566 3832 3546 -286 428.60 3117 -715 3567 3178 3535 357 488.70 3046 -132 

3568 np - - - - - - 

3569 2862 3206 344 533.10 2673 -189 3570 4068 3978 -90 536.90 3441 -627 3571 3467 3619 153 236.60 3383 -84 3572 3182 3572 390 390.00 3182 0 

Adj = Adjusted, Bwt = Bodyweight, np = not pregnant 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 3 - 10000 ppm 

Mean 3410 3615 204 468.79 3146 -265 

SD 301 284 213 94.317 290 213 

N 20 20 20 20 20 20 

Adj = Adjusted, Bwt = Bodyweight 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 4 - 15000 ppm 

4551 3757 3912 155 629.90 3282 -475 4552 3181 3616 435 542.70 3073 -108 4553 3259 3591 333 522.80 3069 -190 4554 3263 3476 213 513.40 2963 -300 4555 3783 4105 322 448.90 3657 -127 4556 3215 3232 17 380.60 2852 -364 4557 3490 3566 76 521.90 3044 -446 4558 3283 3550 267 519.60 3031 -253 4559 3621 4012 392 403.90 3609 -12 4560 3491 3769 278 559.70 3210 -282 4561 3505 3697 192 456.40 3241 -264 4562 3119 3555 436 486.90 3068 -51 4563 3426 3700 274 644.50 3055 -371 4564 3462 3853 391 551.50 3302 -161 4565 3670 3744 74 554.80 3190 -481 4566 3845 3994 149 629.60 3365 -480 4567 3581 3615 34 607.10 3008 -573 4568 3955 4317 362 650.90 3666 -289 

4569 pd 3749x 3138x -610x 88.60x 3050x -699x 

4570 3662 3980 319 447.40 3533 -129 4571 3671 4162 491 502.80 3659 -12 4572 3595 3861 266 563.50 3297 -297 

Adj = Adjusted, Bwt = Bodyweight, pd = premature delivery, x = excluded from calculation 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Gravid Uterine Adjusted Body Weights 

Females (grams) 

Animal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt change

Number Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29 

Group 4 - 15000 ppm 

Mean 3516 3777 261 530.42 3246 -270 

SD 232 260 138 76.468 251 164 

N 21 21 21 21 21 21 

Adj = Adjusted, Bwt = Bodyweight 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 160 

 MA-252E-93-23 Final Report 

Individual Litter Weight Data 

Preface Appendix K 

Individual Litter Fetal and Placental Weights .................................................................161

Individual Fetal Weights ..................................................................................................169

Individual Placental Weights ...........................................................................................177

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 1 – 0 ppm 

1551 5.0 338.5 41.6 43.0 1552 5.3 362.1 41.0 39.8 1553 5.6 394.9 33.8 40.1 1554 4.6 353.4 44.1 44.3 1555 5.2 421.2 39.5 36.8 1556 4.5 372.2 37.1 37.3 1557 4.5 293.5 37.1 33.9 1558 5.4 301.7 46.4 41.8 1559 3.9 180.6 34.9 40.9 1560 5.1 371.8 45.8 47.6 1561 4.8 399.7 38.5 41.4 1562 4.5 213.7 43.6 42.2 1563 3.9 391.2 34.6 31.6 1564 4.8 388.4 42.2 35.5 1565 4.5 362.9 32.5 33.6 1566 4.5 436.9 43.5 36.6 1567 4.1 331.6 34.2 23.9 1568 4.4 360.6 39.0 40.9 1569 5.4 321.0 41.6 29.7 1570 4.8 298.1 37.3 37.2 1571 5.6 343.2 43.7 41.6 1572 4.5 368.4 42.4 40.2 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 1 – 0 ppm 

Mean 4.8 345.7 39.7 38.2 

SD 0.51 61.12 4.11 5.34 

N 22 22 22 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 2 – 2500 ppm 

2551 5.3 308.0 43.3 48.4 2552 5.3 175.1 44.5 41.8 2553 4.3 380.7 37.7 38.6 2554 5.6 297.1 37.3 36.8 2555 4.7 304.1 33.5 34.7 2557 3.9 293.6 35.3 29.3 2558 5.4 216.4 44.5 43.0 2559 4.8 268.2 45.3 44.6 2560 5.8 379.6 43.6 41.0 2561 4.7 361.0 39.9 40.2 2562 4.6 397.3 36.1 36.2 2563 4.6 282.6 39.3 31.3 2564 4.9 382.5 43.0 42.2 2565 4.9 303.6 40.5 35.4 2566 4.9 389.7 38.3 41.6 2567 4.1 383.8 40.3 37.6 2568 5.8 376.8 47.5 46.7 2569 4.1 323.0 32.7 32.2 2570 5.2 331.1 34.9 38.3 2571 4.6 412.3 39.3 42.5 2572 5.2 371.8 35.9 38.0 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 2 – 2500 ppm 

Mean 4.9 330.4 39.7 39.1 

SD 0.54 62.33 4.16 4.92 

N 21 21 21 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 3 – 10000 ppm 

3551 4.9 252.2 45.5 41.3 3552 5.0 418.7 34.7 35.6 3553 5.0 326.2 36.5 35.7 3554 4.1 267.6 28.4 30.8 3555 4.9 306.1 41.5 37.8 3556 4.9 402.3 46.5 44.5 3557 4.6 400.7 39.6 40.6 3558 6.5 321.9 45.0 47.4 3559 5.5 268.8 46.7 42.9 3560 4.5 276.8 34.0 35.2 3561 4.2 296.4 42.0 42.8 3562 4.7 280.0 38.4 44.0 3563 4.6 459.8 34.4 35.7 3565 6.8 309.9 51.5 51.8 3566 5.0 274.5 34.4 33.8 3567 5.5 322.6 45.8 46.4 3569 4.4 381.3 36.3 31.7 3570 5.0 342.2 39.9 37.1 3571 6.4 139.6 46.5 - 3572 4.7 234.5 32.4 35.0 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 3 – 10000 ppm 

Mean 5.1 314.1 40.0 39.5 

SD 0.74 73.41 6.10 5.74 

N 20 20 20 19 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 4 – 15000 ppm 

4551 5.1 414.1 36.5 40.8 4552 5.9 354.0 44.6 43.9 4553 5.1 353.5 43.8 46.9 4554 4.7 378.1 42.0 41.9 4555 4.4 327.0 36.7 36.1 4556 4.8 270.1 46.2 43.8 4557 4.6 325.4 36.5 35.5 4558 4.9 367.1 47.8 37.3 4559 6.5 264.3 54.6 50.2 4560 5.0 390.1 45.2 42.4 4561 5.1 281.0 46.1 48.2 4562 5.3 334.5 39.8 33.9 4563 5.1 452.5 39.7 35.7 4564 4.6 378.0 37.9 37.5 4565 4.3 395.0 36.3 35.8 4566 5.8 389.9 40.7 36.5 4567 5.2 393.4 38.9 39.4 4568 5.1 438.3 42.8 37.4 

4569 pd 4.3x 306.7x 30.7x 30.6x 

4570 5.4 266.4 48.7 42.2 4571 5.1 342.4 42.2 43.1 4572 4.3 378.3 39.0 36.0 

pd = premature delivery, x = excluded from calculations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Litter Fetal and Placental Weights 

 (grams) 

Animal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Ov

Number Mean Weight Mean Mean 

Group 4 – 15000 ppm 

Mean 5.1 356.8 42.2 40.2 

SD 0.54 53.92 4.80 4.65 

N 21 21 21 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 1 – 0 ppm 

1551 42.3 43.9 41.8 42.9 44.7 41.6 40.2 40.8 42.5 1552 40.2 45.7 43.0 39.4 34.2 38.7 43.3 35.6 36.3 45.9 1553 39.5 43.8 40.5 33.8 28.3 40.9 39.0 35.5 42.5 41.3 49.2 

1554 44.2 47.1 46.2 43.1 40.7 42.4 45.5 43.5 44.9 1555 38.3 37.6 42.3 33.2 38.4 37.0 32.8 41.1 34.6 42.8 41.5 40.1 1556 37.2 40.2 33.2 36.1 36.4 37.2 37.6 36.4 36.1 39.0 40.1 1557 36.7 39.1 34.2 38.1 33.9 36.7 36.3 34.9 40.4 1558 43.1 46.9 46.5 40.2 41.2 41.0 39.6 46.3 1559 36.1 35.5 33.0 35.4 35.9 40.9 1560 46.5 44.1 48.7 45.5 55.9 42.1 43.4 43.5 48.6 1561 40.0 48.8 45.9 44.1 39.1 30.2 29.7 34.2 40.3 42.4 44.9 1562 42.8 40.5 44.4 42.8 40.7 45.4 1563 32.6 36.0 28.8 30.4 26.6 23.0 26.3 31.2 30.5 34.8 37.6 42.0 44.0 1564 38.8 47.5 38.5 41.4 29.9 23.2 41.4 43.3 38.2 42.7 42.3 1565 33.0 45.7 38.7 24.2 25.2 23.7 35.9 32.5 25.4 27.5 37.6 46.6 1566 39.7 47.2 43.9 41.2 38.7 34.0 32.7 33.4 43.8 37.0 36.6 48.5 1567 33.2 37.1 37.2 29.7 31.1 27.8 23.9 30.8 39.8 37.6 36.5 1568 40.1 42.1 44.3 41.7 39.9 29.2 40.3 39.8 40.6 42.6 1569 40.1 48.2 40.4 38.0 30.7 29.7 39.7 43.9 50.4 1570 37.3 37.0 38.0 42.2 40.5 29.2 35.5 35.3 40.5 1571 42.9 46.5 47.2 44.9 40.6 36.5 39.0 43.2 45.3 1572 40.9 42.8 40.6 40.3 40.4 32.8 38.5 40.0 46.2 46.8 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 1 – 0 ppm 

Mean 39.3 

SD 3.64 

N 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 2 – 2500 ppm 

2551 44.0 48.4 45.9 42.6 44.4 42.7 37.7 46.3 2552 43.8 41.8 40.1 48.1 45.2 2553 38.0 36.2 34.2 34.9 38.4 39.3 37.5 40.2 38.2 39.8 41.7 

2554 37.2 42.0 26.9 41.4 30.1 36.7 39.7 38.1 42.3 2555 33.8 39.5 31.3 32.3 30.9 30.5 32.2 29.0 37.2 41.2 2557 32.6 40.6 34.4 27.7 22.5 30.3 34.7 30.1 36.4 36.9 2558 43.3 44.3 41.6 41.7 44.3 44.5 2559 44.7 43.6 41.5 44.8 45.3 48.2 44.9 2560 42.2 49.3 46.7 43.5 42.1 38.6 39.2 39.9 49.3 31.1 2561 40.1 42.1 41.5 41.1 36.1 40.3 39.6 33.8 39.2 47.3 2562 36.1 43.6 33.9 33.7 35.2 33.1 41.7 26.5 28.7 37.5 39.7 43.6 2563 35.3 34.8 46.5 39.4 32.1 24.3 26.7 34.4 44.3 2564 42.5 45.3 48.3 49.5 42.6 37.8 38.8 36.9 40.7 42.5 2565 38.0 33.8 36.4 41.2 31.6 37.7 41.0 39.7 42.2 2566 39.0 44.4 44.1 41.0 33.0 32.4 29.7 36.6 42.7 42.1 43.7 2567 38.4 50.0 40.3 41.3 34.1 30.9 31.3 39.9 44.2 39.6 32.1 2568 47.1 53.7 48.4 48.0 46.1 40.0 43.2 47.3 50.1 2569 32.3 35.0 34.4 34.3 30.5 29.4 27.2 28.6 31.9 35.6 36.0 2570 36.8 40.1 42.0 38.1 30.7 38.8 36.1 32.5 35.0 37.6 2571 41.2 47.7 39.7 48.8 37.6 37.7 37.9 37.5 42.6 43.9 38.9 2572 37.2 39.4 38.2 38.1 33.8 34.3 30.8 33.7 36.9 43.8 42.9 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 2 – 2500 ppm 

Mean 39.2 

SD 4.13 

N 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 3 – 10000 ppm 

3551 42.1 45.4 41.0 45.5 38.5 37.3 44.6 3552 34.9 42.1 38.4 37.0 36.3 32.8 25.9 34.8 32.0 23.3 28.2 39.1 48.7 

3553 36.2 39.2 37.2 35.8 35.6 34.9 32.5 37.2 37.0 36.7 3554 29.7 31.6 32.6 35.7 27.5 13.9 26.4 31.8 34.9 33.2 3555 38.3 43.9 33.7 39.8 36.0 33.3 38.8 41.5 39.0 3556 44.7 48.3 43.4 44.2 45.3 45.0 38.3 44.5 46.5 46.7 3557 40.1 44.8 41.8 36.3 34.8 36.5 36.4 40.7 43.6 42.6 43.2 3558 46.0 52.3 45.0 54.1 35.6 41.4 46.7 46.9 3559 44.8 52.1 40.6 40.5 39.7 48.3 47.6 3560 34.6 36.4 32.1 35.8 30.2 35.7 33.5 33.2 39.9 3561 42.3 45.8 43.1 39.4 39.9 40.1 42.6 45.5 3562 40.0 35.0 36.8 39.5 40.8 39.8 44.7 43.4 3563 35.4 44.0 37.6 30.0 30.4 33.7 26.3 26.3 35.3 40.4 41.9 40.0 32.8 41.2 3565 51.7 54.2 50.5 50.9 49.5 50.5 54.3 3566 34.3 38.0 33.8 33.1 34.2 31.4 30.1 35.6 38.3 3567 46.1 47.7 44.8 40.8 47.6 48.2 43.1 50.3 3569 34.7 39.0 37.2 35.0 31.3 30.5 23.8 32.8 33.4 35.9 37.3 45.2 3570 38.0 33.0 41.1 41.9 40.8 39.5 33.1 29.6 44.8 38.5 3571 46.6 46.6 48.5 44.6 3572 33.5 36.3 40.1 30.0 34.5 30.4 24.2 39.1 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 3 – 10000 ppm 

Mean 39.7 

SD 5.67 

N 20 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 4 – 15000 ppm 

4551 37.7 47.2 29.8 34.1 37.6 32.8 31.1 33.1 38.3 40.9 42.9 46.4 

4552 44.2 44.6 43.8 42.7 43.9 47.1 41.7 43.3 46.8 4553 44.2 48.5 35.6 36.8 43.9 45.9 48.3 46.9 47.6 4554 42.0 47.2 43.4 36.7 41.4 37.5 38.1 42.5 41.9 49.4 4555 36.3 38.4 39.0 39.2 37.7 30.9 35.4 29.0 33.1 44.3 4556 45.0 43.5 47.3 42.9 45.1 46.1 45.2 4557 36.2 36.1 35.1 35.0 37.4 34.5 34.5 34.4 38.8 39.7 4558 40.8 47.8 42.0 38.8 35.9 30.4 37.5 45.8 39.2 49.8 4559 52.9 50.5 50.0 54.2 53.4 56.2 4560 43.3 43.5 46.7 48.0 43.7 44.9 37.0 37.7 42.8 45.8 4561 46.9 50.9 45.5 45.6 39.8 49.2 50.1 4562 37.2 42.6 40.2 38.5 33.9 33.4 33.3 35.0 36.4 41.2 4563 37.7 43.9 38.9 42.0 39.3 35.0 33.3 31.7 28.6 35.1 36.1 44.8 43.9 4564 37.8 47.7 41.9 39.9 38.2 28.9 30.6 33.4 37.3 39.1 40.9 4565 35.9 44.0 34.7 32.6 26.2 37.7 39.1 33.5 34.1 39.8 46.6 26.7 4566 39.0 41.7 47.9 42.0 43.0 33.3 27.2 40.9 33.7 37.9 42.4 4567 39.3 37.9 36.3 44.8 42.4 38.9 43.8 37.1 28.4 38.5 45.3 4568 39.9 49.9 47.4 40.7 32.8 33.9 30.0 34.7 37.7 44.3 44.4 42.6 

4569 pd 30.7x 42.5x 33.3x 21.6x 35.0x 39.0x 24.8x 30.4x 21.3x 29.8x 29.1x 

4570 44.4 51.8 42.8 45.7 39.0 45.8 41.3 4571 42.8 47.5 45.8 42.7 40.6 37.7 40.5 43.3 44.4 4572 37.8 45.2 41.9 36.3 35.6 32.3 32.1 34.3 36.9 40.2 43.5 

pd = premature delivery, x = excluded from calculations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Fetal Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 4 – 15000 ppm 

Mean 41.0 

SD 4.30 

N 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 1 – 0 ppm 

1551 5.0 5.1 5.2 5.2 4.7 5.1 5.1 4.5 5.3 1552 5.3 5.9 6.0 5.0 4.1 5.5 4.9 4.6 5.0 7.0 1553 5.6 6.3 5.5 6.8 4.2 5.2 5.2 5.0 5.6 5.7 6.1 1554 4.6 5.9 5.0 3.9 4.2 4.3 4.6 4.2 4.5 1555 5.1 5.4 5.5 4.5 5.1 4.5 4.1 5.0 4.4 5.4 6.7 6.0 1556 4.5 4.4 4.0 4.9 4.3 4.1 4.1 4.2 5.0 5.3 4.9 1557 4.6 4.7 4.4 4.7 3.8 4.3 4.7 4.1 5.7 1558 5.4 5.3 6.4 4.9 4.8 4.8 5.2 6.2 1559 3.9 3.8 3.6 3.5 4.1 4.5 1560 5.1 6.4 5.7 4.9 5.7 4.6 4.3 4.5 5.0 1561 4.8 6.4 5.2 5.3 4.7 4.1 3.7 3.9 4.5 5.0 5.4 1562 4.6 4.6 4.9 4.7 3.9 4.7 1563 3.9 4.2 3.4 3.7 3.0 2.6 3.4 4.2 4.2 3.6 4.0 5.2 5.3 1564 4.8 5.6 4.8 5.9 3.7 3.2 5.0 5.1 4.5 5.4 5.0 1565 4.5 6.4 5.6 3.8 3.4 3.7 4.5 4.5 3.8 3.1 4.9 5.8 1566 4.5 5.3 5.1 4.6 4.5 3.6 3.5 3.8 5.3 4.0 3.7 5.7 1567 4.1 4.8 4.9 3.2 3.4 3.5 2.8 3.6 5.3 4.3 4.8 1568 4.4 4.5 5.4 4.5 3.9 3.1 4.5 4.6 4.7 4.6 1569 5.4 6.4 6.1 4.6 4.5 3.6 5.4 6.1 6.2 1570 4.8 4.4 5.1 5.4 4.7 3.3 4.4 5.3 5.7 1571 5.6 6.2 6.4 6.4 5.3 4.4 5.3 5.4 5.7 1572 4.5 4.8 4.5 3.9 4.6 3.9 4.3 4.4 5.1 4.7 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 1 – 0 ppm 

Mean 4.8 

SD 0.50 

N 22 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 2 – 2500 ppm 

2551 5.3 5.8 5.9 5.0 4.6 5.5 4.7 5.7 2552 5.3 4.7 4.6 6.1 5.6 2553 4.3 4.1 4.5 4.2 4.1 4.5 4.1 4.5 4.3 4.3 4.7 2554 5.6 6.1 4.9 6.3 4.5 5.3 5.6 5.8 6.3 2555 4.7 6.1 4.1 5.1 4.4 4.9 4.5 4.2 4.2 5.2 2557 3.9 5.6 3.7 3.0 2.7 3.7 3.9 3.5 4.5 4.1 2558 5.4 5.4 4.9 4.9 5.4 6.3 2559 4.8 4.5 4.7 5.0 4.5 5.1 4.7 2560 5.8 6.7 7.0 6.0 5.9 4.5 4.9 5.5 6.6 5.5 2561 4.7 5.0 4.8 4.7 4.7 5.1 4.3 4.2 4.9 5.0 2562 4.6 5.5 4.9 4.2 4.8 4.0 5.3 3.7 3.7 4.8 4.4 5.2 2563 4.6 5.7 5.9 4.7 4.2 3.1 3.1 4.3 5.9 2564 4.9 4.5 6.0 6.2 5.1 4.1 4.7 3.9 4.8 4.8 2565 4.9 4.0 4.8 5.4 3.9 4.9 4.9 4.9 6.2 2566 4.9 5.1 5.5 5.2 4.0 4.4 4.0 4.3 5.5 5.3 5.6 2567 4.1 5.0 4.9 4.5 3.3 3.2 3.2 4.3 4.4 4.2 4.4 2568 5.8 7.2 5.9 5.7 5.6 5.1 5.5 5.5 6.0 2569 4.1 4.3 4.2 3.9 3.9 3.5 3.7 3.9 3.7 4.3 5.2 2570 5.2 6.2 5.4 4.8 4.2 5.1 5.1 5.1 4.6 6.4 2571 4.6 5.2 4.3 4.9 4.2 4.2 4.2 4.1 4.6 5.2 5.2 2572 5.2 5.6 5.1 5.2 4.7 5.0 4.0 4.8 5.8 5.8 6.1 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 2 – 2500 ppm 

Mean 4.9 

SD 0.55 

N 21 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 3 – 10000 ppm 

3551 4.9 4.7 6.0 5.0 4.6 4.2 4.8 3552 5.0 5.6 5.3 5.4 4.7 4.5 4.2 4.7 4.4 3.7 4.3 6.1 7.3 3553 5.0 5.5 5.2 5.0 4.1 4.3 5.1 5.2 5.2 5.0 3554 4.1 4.4 4.6 4.6 3.8 2.4 3.3 4.5 4.6 4.9 3555 4.9 5.4 5.1 5.2 4.7 3.9 4.8 5.6 4.8 3556 4.9 5.6 4.9 4.6 4.9 4.6 4.2 5.2 5.1 5.3 3557 4.6 5.6 5.4 4.3 3.7 4.1 3.5 4.5 4.9 5.1 4.9 3558 6.5 7.0 7.0 6.6 6.4 5.9 6.4 6.0 3559 5.5 6.0 5.0 4.8 5.4 5.6 5.9 3560 4.5 4.9 4.0 4.3 3.9 4.4 5.1 4.1 5.4 3561 4.2 4.5 4.4 3.7 4.3 4.0 3.9 4.9 3562 4.7 4.3 4.3 4.6 5.3 5.0 4.7 5.0 3563 4.6 5.8 4.1 3.5 3.8 4.2 3.8 4.3 4.1 5.1 5.0 5.5 5.5 5.1 3565 6.8 7.3 6.2 6.9 6.3 6.6 7.3 3566 5.0 5.3 5.2 5.1 4.9 4.1 4.9 4.8 5.4 3567 5.5 6.0 5.4 4.8 5.3 5.1 5.5 6.3 3569 4.4 4.9 4.9 4.5 4.0 3.8 2.9 4.4 4.3 4.4 4.4 5.4 3570 5.0 4.6 5.8 5.9 5.0 5.2 4.1 3.7 6.1 4.5 3571 6.4 6.4 6.8 6.1 3572 4.7 5.4 5.3 3.8 5.0 4.2 3.7 5.6 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 3 – 10000 ppm 

Mean 5.1 

SD 0.73 

N 20 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 4 – 15000 ppm 

4551 5.1 6.6 4.8 4.9 4.7 4.6 4.3 4.0 4.5 6.3 5.9 5.7 4552 5.9 5.9 6.0 6.2 6.0 6.1 5.5 4.9 6.2 4553 5.1 5.7 4.0 4.0 5.2 5.3 5.9 4.6 5.8 4554 4.7 5.2 4.7 4.3 4.7 3.9 4.5 5.0 4.6 5.1 4555 4.4 4.8 4.5 4.7 4.7 3.5 4.2 3.8 4.2 5.6 4556 4.8 4.8 5.2 4.4 4.7 4.6 4.9 4557 4.6 4.9 4.5 5.0 4.4 4.5 4.4 4.0 4.6 4.9 4558 4.9 5.8 4.9 4.9 4.1 4.4 4.5 5.0 4.6 6.0 4559 6.5 5.9 5.1 8.5 6.8 6.3 4560 5.0 5.1 4.6 5.1 5.0 5.4 4.7 4.5 5.0 5.3 4561 5.1 5.6 4.7 4.7 4.8 4.8 5.7 4562 5.3 6.5 5.5 5.7 4.7 5.1 4.4 4.9 5.2 5.7 4563 5.1 6.3 4.9 5.8 5.4 4.4 4.0 4.3 3.7 4.8 4.7 6.7 6.3 4564 4.7 6.8 4.5 5.3 4.4 2.8 3.5 4.3 4.8 4.7 5.5 4565 4.3 4.9 4.2 4.0 3.0 4.6 4.7 4.3 4.1 5.0 5.6 3.3 4566 5.9 15.3 5.7 5.4 4.8 4.3 1.9 4.8 4.7 5.5 6.1 4567 5.2 4.9 4.5 6.0 5.2 5.5 5.7 4.4 3.6 5.3 6.5 4568 5.1 6.6 5.6 5.1 4.5 4.4 4.2 4.5 4.6 5.5 5.6 5.8 

4569 pd 3.9x 3.9x 3.9x 2.5x 5.7x 5.5x 5.0x 4.9x 1.7x 5.5x miss 

4570 5.4 5.9 4.9 5.7 4.9 5.9 5.3 4571 5.1 5.6 5.2 5.5 5.0 4.6 4.5 5.1 5.5 4572 4.3 4.7 4.9 4.3 3.8 3.5 3.7 3.9 4.7 4.6 5.3 

pd = premature delivery, x = excluded from calculations, miss = missing at time of examination 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

 Individual Placental Weights 

 (grams) 

Animal Litter Fetus 

Number Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 

Group 4 – 15000 ppm 

Mean 5.1 

SD 0.53 

N 21 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 185 

 MA-252E-93-23 Final Report

Individual Fetal Observations Appendix L 

Key to Abbreviations 

av = atrio-ventricular 

vert. = vertebra(e) 

vs = visceral/skeletal evaluations 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1551 8vs 1 F 43.9 vs Variants: 13th bilateral rib, complete  2 M 41.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral, unossified

 Suture/fontanel: frontal suture, plaque of bone  3 M 42.9 vs No abnormality detected

 4 F 44.7 vs Variants: 13th bilateral rib, complete  5 M 41.6 vs Variants: 13th left rib, short  6 M 40.2 vs No abnormality detected 7 F 40.8 vs Variants: 13th right rib, short  8 F 42.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete  1552 9vs 1 F 45.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  2 M 43.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 3 F 39.4 vs Limb bones: metacarpal, 1st digit, right, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 4 M 34.2 vs Limb bones: epiphyses, incompletely ossified

 5 F 38.7 vs No abnormality detected

 6 F 43.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 7 F 35.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1552 9vs 7 F 35.6 vs Thorax: subclavian artery, left side, additional  Variants: 13th bilateral rib, complete  8 F 36.3 vs Thorax: subclavian artery, branching variation, left side  9 M 45.9 vs No abnormality detected

 1553 10vs 1 F 43.8 vs Variants: 13th bilateral rib, short  2 F 40.5 vs Thorax: common carotid artery, displaced, left side  Variants: 13th left rib, complete  Variants: 13th right rib, short  3 M 33.8 vs Variants: sternebra(e), 6th, unossified 4 F 28.3 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 5 F 40.9 vs No abnormality detected

 6 F 39.0 vs Variants: sternebra(e), 6th, unossified 7 F 35.5 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 8 F 42.5 vs No abnormality detected

 9 F 41.3 vs Variants: 13th bilateral rib, short  10 F 49.2 vs Variants: 13th left rib, complete  Variants: 13th right rib, rudimentary 

Variants: sternebra(e), 6th, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1554 8vs 1 M 47.1 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

 2 M 46.2 vs Suture/fontanel: frontal suture, plaque of bone 

 Variants: 13th bilateral rib, complete 

 3 F 43.1 vs Thoracic vert.: 20 thoracolumbar vertebrae, present 

 Variants: 13th bilateral rib, complete 

 4 M 40.7 vs Variants: 13th left rib, complete 

 Variants: 13th right rib, short  5 M 42.4 vs Girdles: pelvic girdle, right, unilateral caudal shift  Variants: 13th bilateral rib, complete  6 F 45.5 vs Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 7 F 43.5 vs No abnormality detected

 8 F 44.9 vs Suture/fontanel: frontal suture, plaque of bone  Variants: 13th bilateral rib, complete  1555 11vs 1 M 37.6 vs LUMBAR VERT.: LUMBAR REGION, SCOLIOSIS  LUMBAR VERT.: LUMBAR VERTEBRAL ARCH(ES), 1ST, RIGHT, ABSEN

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossified

 Lumbar vert.: lumbar vertebral arch(es), 2nd-3rd, right, misaligned 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1555 11vs 1 M 37.6 vs Lumbar vert.: lumbar vertebral centrum(a), 1st-2nd, misaligned  Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 2 M 42.3 vs Head: hyoid cornua, right, bent inwards  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 3 F 33.2 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  4 F 38.4 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 5 F 37.0 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 6 F 32.8 vs Variants: 13th right rib, complete  7 M 41.1 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Sternebrae: sternebral center(s), 6th, bipartite ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1555 11vs 8 M 34.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 9 F 42.8 vs Head: hyoid cornua, bilateral, bent inwards  Variants: 13th left rib, complete  Variants: 13th right rib, short  10 M 41.5 vs Head: parietal, left, unossified area(s) 

Limb bones: epiphyses, incompletely ossified

 Liver: gallbladder, rudimentary  Lungs: accessory lung lobe, absent  Sternebrae: sternebral center(s), 1st, supernumerary ossification  Variants: 13th right rib, short  11 M 40.1 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Thorax: common carotid artery, displaced, left side  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 1556 10vs 1 M 40.2 vs Thorax: subclavian artery, branching variation, left side  2 M 33.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 3 F 36.1 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1556 10vs 3 F 36.1 vs Limb bones: epiphyses, incompletely ossified

 4 F 36.4 vs Thorax: subclavian artery, left side, additional  5 F 37.2 vs Thorax: common carotid artery, displaced, left side  Thorax: subclavian artery, branching variation, left side  Variants: 13th left rib, rudimentary, non-articulated  Variants: 13th right rib, short  6 F 37.6 vs Variants: 13th right rib, short  7 F 36.4 vs Thorax: subclavian artery, left side, additional  8 M 36.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thorax: common carotid artery, displaced, left side 

 Variants: 13th right rib, complete  9 M 39.0 vs Variants: 13th right rib, short, non-articulated  10 F 40.1 vs Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 1557 8vs 1 M 39.1 vs Variants: 13th bilateral rib, complete  2 M 34.2 vs Head: frontal, right, unossified area(s)  Head: parietal, right, unossified area(s) 

Limb bones: epiphyses, incompletely ossified

 3 M 38.1 vs Head: parietal, right, unossified area(s)  Variants: 13th right rib, complete  4 F 33.9 vs Head: frontal, right, unossified area(s)  Variants: 13th bilateral rib, complete  5 M 36.7 vs Cervical vert.: odontoid process, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1557 8vs 5 M 36.7 vs Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 6 M 36.3 vs Head: parietal, right, unossified area(s)

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  7 M 34.9 vs Variants: 13th left rib, short  Variants: 13th right rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 8 M 40.4 vs Variants: 13th bilateral rib, complete  1558 7vs 1 F 46.9 vs Variants: 13th bilateral rib, short 

 2 M 46.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 3 F 40.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  4 F 41.2 vs Variants: 13th bilateral rib, short  5 F 41.0 vs Liver: gallbladder, rudimentary  Variants: 13th bilateral rib, short  6 F 39.6 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

 Variants: 13th left rib, rudimentary  Variants: 13th right rib, short  7 M 46.3 vs Head: frontal, left, unossified area(s)  Variants: 13th left rib, short 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1559 5vs 1 M 35.5 vs Variants: 13th bilateral rib, complete  2 M 33.0 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

 3 M 35.4 vs Limb bones: epiphyses, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  4 M 35.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 5 F 40.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  1560 8vs 1 M 44.1 vs Variants: 13th bilateral rib, complete  2 M 48.7 vs Variants: 13th bilateral rib, complete  3 M 45.5 vs Girdles: clavicle, bilateral, bent  Head: interparietal, supraoccipital, partially fused  Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  4 F 55.9 vs Variants: 13th bilateral rib, complete  5 M 42.1 vs Variants: 13th left rib, short  Variants: 13th right rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1560 8vs 6 F 43.4 vs Girdles: clavicle, right, bent  Variants: 13th left rib, short  Variants: 13th right rib, rudimentary, non-articulated  7 F 43.5 vs Variants: 13th bilateral rib, complete  8 M 48.6 vs Variants: 13th bilateral rib, complete  1561 10vs 1 F 48.8 vs Head: anterior supraorbital notch, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  2 F 45.9 vs Sternebrae: sternebral center(s), 3rd-4th, bridge of ossification  Sternebrae: sternebral center(s), 4th-5th, bridge of ossification  3 M 44.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 4 M 39.1 vs Head: anterior supraorbital notch, bilateral, incompletely ossified

 Sternebrae: sternebral center(s), 4th-5th, bridge of ossification  Variants: 13th bilateral rib, short  5 M 30.2 vs Head: anterior supraorbital notch, bilateral, incompletely ossified

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: talus, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 6 F 29.7 vs Brain: lateral ventricle, bilateral, dilated 

Limb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: talus, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1561 10vs 6 F 29.7 vs Sternebrae: sternebral center(s), 4th-5th, bridge of ossification 

 Variants: 13th left rib, complete  Variants: 13th right rib, short  7 M 34.2 vs Head: anterior supraorbital notch, right, incompletely ossified

 8 F 40.3 vs Head: anterior supraorbital notch, left, incompletely ossified

 Sternebrae: sternebral center(s), 3rd-4th, bridge of ossification  Sternebrae: sternebral center(s), 4th-5th, fused  9 F 42.4 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Sternebrae: sternebral center(s), 3rd-4th, bridge of ossification  Sternebrae: sternebral center(s), 4th-5th, bridge of ossification  10 M 44.9 vs Head: anterior supraorbital notch, bilateral, incompletely ossified

 Liver: gallbladder, rudimentary  Suture/fontanel: frontal suture, plaque of bone  1562 5vs 1 F 40.5 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thorax: subclavian artery, left side, additional  Variants: 13th bilateral rib, complete  2 M 44.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  3 M 42.8 vs Head: hyoid cornua, left, bent inwards 

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1562 5vs 3 M 42.8 vs Variants: 13th bilateral rib, complete  4 F 40.7 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete 

 5 F 45.4 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete  1563 12vs 1 F 36.0 vs Variants: 13th left rib, short  Variants: 13th right rib, rudimentary  2 F 28.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 3 F 30.4 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, unossified

 4 F 26.6 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 5 F 23.0 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1563 12vs 5 F 23.0 vs Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, unossified

 6 F 26.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 7 M 31.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Thorax: subclavian artery, branching variation, left side  Variants: 13th right rib, short  8 M 30.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thorax: subclavian artery, branching variation, left side 

Variants: sternebra(e), 5th, unossified

 9 M 34.8 vs Cervical: thymus gland, partially undescended, left side 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 10 F 37.6 vs Thorax: subclavian artery, left side, additional 

Variants: sternebra(e), 5th, unossified

 11 M 42.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 12 F 44.0 vs Variants: sternebra(e), 5th, unossified 1564 10vs 1 M 47.5 vs Variants: 13th right rib, short  2 F 38.5 vs Girdles: pelvic girdle, left, unilateral caudal shift 

Limb bones: epiphyses, unossified

 Variants: 13th bilateral rib, complete  3 M 41.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1564 10vs 3 M 41.4 vs Variants: 13th bilateral rib, complete  4 F 29.9 vs Girdles: pelvic girdle, right, unilateral cranial shift  Variants: 13th bilateral rib, complete  5 F 23.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 6 M 41.4 vs Sternebrae: omosternum, left, ossified

 7 F 43.3 vs Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossified

 8 M 38.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, short  9 F 42.7 vs Head: frontal, left, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete  10 M 42.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 1565 11vs 1 M 45.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 2 M 38.7 vs No abnormality detected

 3 M 24.2 vs Head: hyoid cornua, bilateral, bent inwards 

Girdles: pubis, bilateral, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1565 11vs 3 M 24.2 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Suture/fontanel: anterior fontanelle, enlarged  Suture/fontanel: posterior fontanelle, enlarged  4 M 25.2 vs Cervical vert.: cervical rib, right, without costal cartilage 

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses, unossified

 Variants: 13th left rib, short, non-articulated  5 M 23.7 vs Girdles: pelvic girdle, right, unilateral caudal shift 

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossified

 Suture/fontanel: posterior fontanelle, enlarged  Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 6 F 35.9 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short, non-articulated 

Variants: sternebra(e), 6th, incompletely ossified

 7 F 32.5 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1565 11vs 7 F 32.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 8 F 25.4 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 9 F 27.5 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 10 M 37.6 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, short  11 F 46.6 vs Variants: sternebra(e), 5th, incompletely ossified

 1566 11vs 1 M 47.2 vs No abnormality detected

 2 M 43.9 vs Thorax: subclavian artery, left side, additional 

Variants: sternebra(e), 5th, unossified

 3 F 41.2 vs Variants: 13th bilateral rib, complete  4 F 38.7 vs Lungs: accessory lung lobe, small  5 M 34.0 vs Variants: 13th left rib, short  Variants: 13th right rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1566 11vs 6 F 32.7 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 7 F 33.4 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 8 M 43.8 vs Variants: 13th bilateral rib, complete 

 9 F 37.0 vs Variants: 13th bilateral rib, complete 

 10 F 36.6 vs Lungs: accessory lung lobe, rudimentary 

Variants: sternebra(e), 5th, incompletely ossified

 11 M 48.5 vs Variants: 13th bilateral rib, complete  1567 10vs 1 M 37.1 vs Variants: 13th bilateral rib, complete  2 M 37.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  3 M 29.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  4 M 31.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1567 10vs 4 M 31.1 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  5 M 27.8 vs Head: frontal, left, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  6 F 23.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  7 M 30.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  8 M 39.8 vs THORAX: AORTIC ARCH, INTERRUPTED  Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  9 M 37.6 vs Limb bones: 2nd phalanx, 1st digit, left forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

 Variants: 13th bilateral rib, complete  10 M 36.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1568 9vs 1 F 42.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 2 M 44.3 vs No abnormality detected

 3 F 41.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 4 F 39.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Suture/fontanel: nasal suture, sutural bone  5 M 29.2 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 6 F 40.3 vs Head: frontal, right, unossified area(s) 

Variants: sternebra(e), 5th, unossified

 7 M 39.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th left rib, rudimentary, non-articulated  Variants: 13th right rib, short  8 F 40.6 vs No abnormality detected

 9 M 42.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 1569 8vs 1 M 48.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  2 M 40.4 vs Head: frontal, right, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  3 M 38.0 vs Head: frontal, right, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 4 M 30.7 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1569 8vs 4 M 30.7 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 5 F 29.7 vs Variants: 13th left rib, complete  Variants: 13th right rib, rudimentary  6 M 39.7 vs Head: frontal, left, unossified area(s)  7 M 43.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short  8 M 50.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short  1570 8vs 1 F 37.0 vs Girdles: pelvic girdle, right, unilateral cranial shift 

 Heart: atrium, bilateral, distended  Variants: 13th bilateral rib, short  2 F 38.0 vs Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 3 M 42.2 vs Variants: sternebra(e), 6th, incompletely ossified

 4 F 40.5 vs Suture/fontanel: posterior fontanelle, enlarged 

Variants: sternebra(e), 6th, incompletely ossified

 5 M 29.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Suture/fontanel: anterior fontanelle, enlarged  Suture/fontanel: posterior fontanelle, enlarged 

Variants: sternebra(e), 6th, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1570 8vs 6 F 35.5 vs No abnormality detected

 7 F 35.3 vs Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, unossified

 8 M 40.5 vs No abnormality detected

 1571 8vs 1 M 46.5 vs Variants: sternebra(e), 5th, incompletely ossified

 2 M 47.2 vs Head: hyoid cornua, right, bent inwards 

Sternebrae: omosternum, left, ossified

 3 M 44.9 vs Variants: sternebra(e), 5th, unossified 4 F 40.6 vs Head: frontal, left, unossified area(s) 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 5 M 36.5 vs Cervical vert.: odontoid process, incompletely ossified

Variants: sternebra(e), 5th, unossified

 6 F 39.0 vs Head: hyoid cornua, right, bent outwards  Variants: 13th left rib, complete  Variants: 13th right rib, short  7 M 43.2 vs BRAIN: LATERAL VENTRICLE, BILATERAL, HYDROCEPHALY  Suture/fontanel: anterior fontanelle, enlarged  8 F 45.3 vs Cervical vert.: odontoid process, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 1572 9vs 1 F 42.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 1 - 0 ppm  1572 9vs 1 F 42.8 vs Variants: sternebra(e), 6th, incompletely ossified

 2 M 40.6 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

 3 F 40.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  4 M 40.4 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, short  5 F 32.8 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, rudimentary, non-articulated  6 F 38.5 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 7 F 40.0 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short  8 M 46.2 vs Variants: 13th right rib, short  9 F 46.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2551 7vs 1 F 48.4 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 2 M 45.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: frontal suture, plaque of bone  3 M 42.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th right rib, complete  4 M 44.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 5 M 42.7 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 6 M 37.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 7 M 46.3 vs No abnormality detected

 2552 4vs 1 F 41.8 vs Variants: 13th right rib, short, non-articulated  2 M 40.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, complete  3 M 48.1 vs Variants: 13th bilateral rib, complete  4 M 45.2 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2553 10vs 1 M 36.2 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 2 M 34.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 3 F 34.9 vs Cervical: thymus gland, enlarged 

 Heart: pericardium, distended  Variants: 13th left rib, short 

Variants: sternebra(e), 5th, unossified

 4 M 38.4 vs Lungs: accessory lung lobe, absent  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 5 M 39.3 vs Variants: 13th left rib, short 

 Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, unossified

 6 F 37.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

 7 F 40.2 vs Cervical: thymus gland, partially undescended, left side 

Variants: sternebra(e), 5th, unossified

 8 M 38.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 9 M 39.8 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified Variants: 13th left rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2553 10vs 9 M 39.8 vs Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 10 F 41.7 vs Variants: 13th left rib, short  Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 2554 8vs 1 M 42.0 vs Head: frontal, left, unossified area(s)  2 M 26.9 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete  3 M 41.4 vs Head: frontal, left, unossified area(s)  Variants: 13th left rib, short  Variants: 13th right rib, complete  4 F 30.1 vs No abnormality detected

 5 M 36.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 6 M 39.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 7 F 38.1 vs Variants: 13th left rib, short  8 F 42.3 vs Variants: 13th left rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2554 8vs 8 F 42.3 vs Variants: 13th right rib, short  2555 9vs 1 M 39.5 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Variants: 13th left rib, short  2 M 31.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 3 M 32.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 6th, incompletely ossified

 4 M 30.9 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  5 M 30.5 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  6 F 32.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2555 9vs 7 M 29.0 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  8 F 37.2 vs Head: interparietal, supraoccipital, fused 

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 9 M 41.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 2557 9vs 1 M 40.6 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 Thorax: subclavian artery, branching variation, left side 

Variants: sternebra(e), 6th, incompletely ossified

 2 M 34.4 vs Girdles: pelvic girdle, right, unilateral caudal shift 

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 3 F 27.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th left rib, short, non-articulated 

Variants: sternebra(e), 6th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2557 9vs 4 F 22.5 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 5 M 30.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 6 M 34.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 6th, unossified

 7 F 30.1 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 6th, unossified

 8 M 36.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, complete  9 F 36.9 vs Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  2558 5vs 1 F 44.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 2 F 41.6 vs Cervical: thymus gland, bilateral, partially undescended 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 3 F 41.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2558 5vs 3 F 41.7 vs Sternebrae: sternebral center(s), 4th-5th, bridge of ossification 

 Suture/fontanel: posterior fontanelle, enlarged 

Variants: sternebra(e), 5th, incompletely ossified

 4 F 44.3 vs Cervical: thymus gland, partially undescended, left side 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 5 M 44.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Ribs: 8th, right, branched  Thoracic vert.: 18 thoracolumbar vertebrae, present  2559 6vs 1 F 43.6 vs Variants: 13th bilateral rib, complete  2 F 41.5 vs No abnormality detected

 3 F 44.8 vs Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th left rib, short  Variants: 13th right rib, complete  4 M 45.3 vs Girdles: pelvic girdle, right, unilateral caudal shift  Variants: 13th bilateral rib, complete  5 F 48.2 vs Variants: 13th bilateral rib, complete  6 F 44.9 vs Variants: 13th bilateral rib, complete  2560 9vs 1 F 49.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, short 

 2 M 46.7 vs Head: hyoid cornua, left, bent outwards 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2560 9vs 2 M 46.7 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Lungs: accessory lung lobe, absent  Variants: 13th left rib, complete  Variants: 13th right rib, short  3 F 43.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th bilateral rib, complete  4 F 42.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th bilateral rib, complete  5 M 38.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 6 F 39.2 vs Head: hyoid cornua, right, bent outwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: parietal suture, sutural bone  Variants: 13th bilateral rib, complete  7 M 39.9 vs Girdles: pelvic girdle, right, unilateral cranial shift  Variants: 13th bilateral rib, complete  8 M 49.3 vs Head: frontal, left, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short  9 F 31.1 vs Abdomen: spleen, small 

 Abdomen/trunk: umbilicus, hernia

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, left, incompletely ossifiedLimb bones: talus, right, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2560 9vs 9 F 31.1 vs Limb bones: 1st phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: 2nd phalanx, 1st digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 2nd digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 2nd digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, unossified

 Lungs: accessory lung lobe, absent  Variants: 13th bilateral rib, complete  2561 9vs 1 M 42.1 vs Variants: 13th left rib, complete  Variants: 13th right rib, short, floating  2 M 41.5 vs Variants: 13th bilateral rib, complete  3 F 41.1 vs Head: anterior supraorbital notch, left, incompletely ossified

 Variants: 13th bilateral rib, complete  4 M 36.1 vs Thorax: subclavian artery, branching variation, left side  Variants: 13th bilateral rib, complete  5 F 40.3 vs Variants: 13th left rib, complete  Variants: 13th right rib, short  6 F 39.6 vs Head: anterior supraorbital notch, left, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2561 9vs 6 F 39.6 vs Variants: 13th bilateral rib, short 

 7 F 33.8 vs Head: anterior supraorbital notch, right, incompletely ossified

 Variants: 13th bilateral rib, complete  8 F 39.2 vs Variants: 13th bilateral rib, complete  9 F 47.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  2562 11vs 1 M 43.6 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, rudimentary, non-articulated  2 M 33.9 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  3 F 33.7 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  4 F 35.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th bilateral rib, complete  5 M 33.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2562 11vs 6 M 41.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  7 M 26.5 vs Girdles: pubis, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Thorax: subclavian artery, left side, additional  8 F 28.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  9 M 37.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thorax: subclavian artery, branching variation, left side  Variants: 13th bilateral rib, complete  10 F 39.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 11 F 43.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2563 8vs 1 F 34.8 vs Cervical: thymus gland, bilateral, partially undescended 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Sternebrae: sternebral center(s), 4th, misaligned  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 2 M 46.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 3 F 39.4 vs Suture/fontanel: parietal suture, sutural bone 

 Variants: 13th left rib, rudimentary  Variants: 13th right rib, short  4 M 32.1 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 5 F 24.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2563 8vs 5 F 24.3 vs Variants: sternebra(e), 6th, unossified 6 F 26.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, rudimentary 

Variants: sternebra(e), 6th, unossified

 7 M 34.4 vs Variants: 13th bilateral rib, short 

Variants: sternebra(e), 6th, incompletely ossified

 8 M 44.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 6th, unossified

 2564 9vs 1 F 45.3 vs Variants: 13th bilateral rib, complete  2 F 48.3 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified Variants: 13th left rib, short 

Variants: sternebra(e), 6th, incompletely ossified

 3 M 49.5 vs Variants: 13th right rib, short  4 M 42.6 vs Girdles: pelvic girdle, right, unilateral cranial shift  Variants: 13th bilateral rib, complete  5 F 37.8 vs Cervical vert.: odontoid process, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 6 F 38.8 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2564 9vs 6 F 38.8 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 7 M 36.9 vs Lungs: accessory lung lobe, absent  Variants: 13th right rib, rudimentary, non-articulated  8 F 40.7 vs Ribs: 13th, right, interrupted  Variants: 13th left rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 9 F 42.5 vs Variants: sternebra(e), 5th, incompletely ossified

 2565 8vs 1 F 33.8 vs Cervical vert.: cervical rib, bilateral, without costal cartilage 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 2 F 36.4 vs Head: interparietal, incompletely ossified

 Head: interparietal, supraoccipital, bridge of ossification 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 3 M 41.2 vs Thoracic vert.: 20 thoracolumbar vertebrae, present 

 Variants: 13th left rib, short, non-articulated  4 F 31.6 vs Head: interparietal, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2565 8vs 5 M 37.7 vs Head: interparietal, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 6 M 41.0 vs ABDOMEN/TRUNK: ABDOMEN, GASTROSCHISIS

 Cervical vert.: cervical rib, bilateral, without costal cartilage 

Variants: sternebra(e), 4th, unossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 7 F 39.7 vs Variants: sternebra(e), 5th, unossified 8 M 42.2 vs Head: interparietal, supraoccipital, bridge of ossification 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 2566 10vs 1 M 44.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

 2 M 44.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  3 F 41.0 vs Variants: 13th bilateral rib, complete  4 M 33.0 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 Variants: 13th bilateral rib, complete  5 M 32.4 vs Head: frontal, right, unossified area(s) 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2566 10vs 5 M 32.4 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 6th, unossified

 6 M 29.7 vs Variants: 13th left rib, short  7 M 36.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  8 M 42.7 vs Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 9 F 42.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 10 M 43.7 vs Variants: 13th bilateral rib, short  2567 10vs 1 F 50.0 vs Suture/fontanel: anterior fontanelle, enlarged 

Variants: sternebra(e), 5th, incompletely ossified

 2 F 40.3 vs Variants: sternebra(e), 5th, unossified 3 M 41.3 vs Variants: sternebra(e), 6th, incompletely ossified

 4 F 34.1 vs Variants: sternebra(e), 5th, unossified 5 F 30.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Sternebrae: sternebral center(s), 6th, bipartite ossified

 6 F 31.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2567 10vs 6 F 31.3 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th right rib, short  7 M 39.9 vs Head: frontal, left, unossified area(s)  8 F 44.2 vs No abnormality detected

 9 M 39.6 vs Variants: sternebra(e), 5th, incompletely ossified

 10 F 32.1 vs Head: frontal, bilateral, suture within, partially fused  Head: hyoid cornua, right, bent inwards 

Head: hyoid cornua, left, unossified

 Sternebrae: sternebral center(s), 1st, cranial to, supernumerary ossification 

Sternebrae: sternebral center(s), 2nd, bipartite ossified

 Suture/fontanel: frontal/parietal suture, bilateral, irregular  Suture/fontanel: nasal suture, sutural bone  Suture/fontanel: parietal suture, sutural bone  Variants: 13th left rib, short 

Variants: sternebra(e), 6th, incompletely ossified

 2568 8vs 1 M 53.7 vs Head: frontal, right, unossified area(s)  Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 2 M 48.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, short 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2568 8vs 3 M 48.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Suture/fontanel: frontal suture, sutural bone  Suture/fontanel: parietal suture, sutural bone  Variants: 13th left rib, short  Variants: 13th right rib, short, non-articulated  4 F 46.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 5 M 40.0 vs Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 6 F 43.2 vs Head: hyoid cornua, left, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: parietal suture, irregular  7 F 47.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Suture/fontanel: parietal suture, irregular 

Variants: sternebra(e), 5th, unossified

 8 F 50.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 2569 10vs 1 F 35.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, unossified

 Suture/fontanel: anterior fontanelle, enlarged  2 F 34.4 vs Cervical: thymus gland, partially undescended, right side 

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2569 10vs 2 F 34.4 vs Sternebrae: sternebral center(s), 1st, cranial to, supernumerary ossification 

 Suture/fontanel: anterior fontanelle, enlarged  Variants: 13th right rib, short  3 F 34.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Lungs: accessory lung lobe, absent  4 F 30.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  5 M 29.4 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Thorax: subclavian artery, branching variation, left side  6 F 27.2 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: metacarpal, 1st digit, left, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 7 F 28.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 2nd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2569 10vs 7 F 28.6 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossified

 Lungs: accessory lung lobe, absent  8 F 31.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short, non-articulated  9 F 35.6 vs Limb bones: 2nd phalanx, 2nd digit, bilateral forelimb, incompletely ossified

Limb bones: 2nd phalanx, 3rd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossified

 10 M 36.0 vs Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 2570 9vs 1 F 40.1 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2570 9vs 1 F 40.1 vs Liver: gallbladder, rudimentary 

Variants: sternebra(e), 5th, unossified

 2 F 42.0 vs Head: frontal, bilateral, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 3 F 38.1 vs Head: hyoid cornua, left, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 4 M 30.7 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 2nd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 5 M 38.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 6 F 36.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Limb bones: epiphyses, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2570 9vs 6 F 36.1 vs Variants: sternebra(e), 5th, unossified 7 M 32.5 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, right, incompletely ossifiedLimb bones: metacarpal, 1st digit, left, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 8 F 35.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 9 M 37.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 2571 10vs 1 F 47.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 2 F 39.7 vs Limb bones: metacarpal, 1st digit, right, unossified

Limb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 3 F 48.8 vs Head: anterior supraorbital notch, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2571 10vs 4 M 37.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 5 M 37.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Thorax: subclavian artery, left side, additional  6 M 37.9 vs GIRDLES: CLAVICLE, LEFT, BENT 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 7 F 37.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 8 F 42.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 9 M 43.9 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 10 F 38.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 2572 10vs 1 M 39.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 2 F 38.2 vs Variants: sternebra(e), 5th, unossified

 3 F 38.1 vs Cervical: thymus gland, partially undescended, left side 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, short  4 M 33.8 vs Head: premaxilla, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

 5 F 34.3 vs Limb bones: epiphyses, incompletely ossified

 Suture/fontanel: frontal suture, plaque of bone 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 2 - 2500 ppm  2572 10vs 5 F 34.3 vs Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, short  6 F 30.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 7 M 33.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

 8 M 36.9 vs Cervical: thymus gland, partially undescended, right side 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete  9 F 43.8 vs Lungs: accessory lung lobe, absent  10 F 42.9 vs Lungs: accessory lung lobe, absent  Variants: 13th left rib, short, non-articulated 

Variants: sternebra(e), 5th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3551 6vs 1 F 45.4 vs Variants: 13th bilateral rib, complete  2 F 41.0 vs Variants: 13th left rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 3 M 45.5 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 4 F 38.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: posterior fontanelle, enlarged  Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 5 F 37.3 vs Head: hyoid cornua, right, bent inwards

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 6 F 44.6 vs Variants: sternebra(e), 5th, unossified 3552 12vs 1 M 42.1 vs Variants: 13th bilateral rib, short 

 2 F 38.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, short  3 M 37.0 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

 4 F 36.3 vs No abnormality detected

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3552 12vs 5 M 32.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 6 M 25.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 7 M 34.8 vs Variants: 13th right rib, short  8 F 32.0 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: metacarpal, 1st digit, left, unossified

 Thorax: subclavian artery, left side, additional 

Variants: sternebra(e), 5th, incompletely ossified

 9 M 23.3 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 10 M 28.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Thorax: subclavian artery, left side, additional  11 M 39.1 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified Variants: 13th left rib, short  Variants: 13th right rib, complete  12 M 48.7 vs No abnormality detected

 3553 9vs 1 M 39.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3553 9vs 2 M 37.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 3 M 35.8 vs Head: anterior supraorbital notch, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short, non-articulated 

Variants: sternebra(e), 5th, incompletely ossified

 4 F 35.6 vs Head: anterior supraorbital notch, left, incompletely ossified

 Variants: 13th bilateral rib, complete  5 F 34.9 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

 6 M 32.5 vs Head: anterior supraorbital notch, right, incompletely ossified

 Head: frontal, right, unossified area(s) 

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 7 M 37.2 vs Head: anterior supraorbital notch, right, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  8 M 37.0 vs Head: anterior supraorbital notch, left, incompletely ossified

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 9 F 36.7 vs Head: hyoid cornua, bilateral, bent inwards 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3553 9vs 9 F 36.7 vs Head: jugal, right, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short, non-articulated  3554 9vs 1 M 31.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified

Variants: sternebra(e), 1st, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 2 F 32.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Thorax: common carotid artery, branching variation, left side  Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 3 F 35.7 vs Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 4 F 27.5 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: metacarpal, 1st digit, left, unossified

 Liver: gallbladder, rudimentary 

Variants: sternebra(e), 5th, unossified

 5 M 13.9 vs Cervical: thymus gland, small 

Girdles: pubis, bilateral, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3554 9vs 5 M 13.9 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Suture/fontanel: posterior fontanelle, enlarged  Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 6 F 26.4 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 7 F 31.8 vs Limb bones: metacarpal, 1st digit, right, unossified

Limb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete  8 M 34.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Variants: 13th bilateral rib, complete  9 M 33.2 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3555 8vs 1 F 43.9 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 2 F 33.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  3 F 39.8 vs GIRDLES: CLAVICLE, LEFT, BENT 

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified

Thoracic vert.: thoracic vert. centrum(a), 2nd, bipartite ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 4 F 36.0 vs No abnormality detected

 5 F 33.3 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

 Variants: 13th right rib, short  6 F 38.8 vs GIRDLES: CLAVICLE, RIGHT, BENT  Variants: 13th left rib, complete  Variants: 13th right rib, short  7 M 41.5 vs Sternebrae: sternebral center(s), 4th-5th, bridge of ossification  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 8 F 39.0 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Sternebrae: sternebral center(s), 4th-5th, bridge of ossification  Variants: 13th bilateral rib, complete  3556 9vs 1 F 48.3 vs Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3556 9vs 2 F 43.4 vs Thorax: subclavian artery, left side, additional  Variants: 13th bilateral rib, complete  3 F 44.2 vs Variants: 13th bilateral rib, complete  4 F 45.3 vs Variants: 13th bilateral rib, complete  5 F 45.0 vs Variants: 13th bilateral rib, complete  6 F 38.3 vs Variants: 13th left rib, complete  Variants: 13th right rib, short  7 F 44.5 vs Variants: 13th bilateral rib, complete  8 M 46.5 vs Variants: 13th bilateral rib, short  9 F 46.7 vs Variants: 13th bilateral rib, complete  3557 10vs 1 F 44.8 vs Head: anterior supraorbital notch, left, incompletely ossified

 Head: frontal, left, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, complete 

Variants: sternebra(e), 6th, unossified

 2 M 41.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Suture/fontanel: parietal suture, irregular  Variants: 13th left rib, complete 

Variants: sternebra(e), 6th, unossified

 3 F 36.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 6th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3557 10vs 4 F 34.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete  5 M 36.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th bilateral rib, short, non-articulated  6 M 36.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 6th, incompletely ossified

 7 M 40.7 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, rudimentary, non-articulated  8 F 43.6 vs Cervical: thymus gland, partially undescended, left side 

Limb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Lungs: left lung lobe, cyst 

Variants: sternebra(e), 6th, unossified

 9 M 42.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 6th, incompletely ossified

 10 F 43.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 3558 7vs 1 M 52.3 vs Cervical: thymus gland, partially undescended, left side 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3558 7vs 1 M 52.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, short  2 M 45.0 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 3 F 54.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete  4 M 35.6 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Sternebrae: sternebral center(s), 5th-6th, supernumerary ossification, between  Variants: 13th bilateral rib, complete  5 F 41.4 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 6 F 46.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 7 M 46.9 vs Cervical vert.: odontoid process, unossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: parietal suture, sutural bone  Variants: 13th bilateral rib, complete  3559 6vs 1 M 52.1 vs No abnormality detected

 2 F 40.6 vs HEART: VENTRICULAR SEPTUM, SMALL, MEMBRANOUS, VENTRICUL

Cervical vert.: cervical hemicentrum(a), 4th, left, unossified

 Eye: eye, bilateral, small 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3559 6vs 2 F 40.6 vs Head: interparietal, incompletely ossified

 Heart: heart, malrotated 

Limb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Lungs: accessory lung lobe, absent  Lungs: lungs (general), small  Sternebrae: sternebral center(s), 2nd-3rd, supernumerary ossification, partially fu Sternebrae: sternebral center(s), 3rd-4th, bridge of ossification  Thoracic vert.: 20 thoracolumbar vertebrae, present  Thorax: caudal vena cava, distended, malpositioned  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 3 M 40.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Suture/fontanel: parietal suture, sutural bone  Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  4 F 39.7 vs Cervical vert.: odontoid process, ossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3559 6vs 4 F 39.7 vs Liver: gallbladder, rudimentary  Lungs: accessory lung lobe, absent  Suture/fontanel: parietal suture, sutural bone  Thoracic vert.: 20 thoracolumbar vertebrae, present 

Thoracic vert.: thoracic vert. centrum(a), 8th, incompletely ossified

 Variants: 13th bilateral rib, complete  5 F 48.3 vs Head: premaxilla, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  6 M 47.6 vs Head: premaxilla, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Lungs: accessory lung lobe, small  Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  3560 8vs 1 M 36.4 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 2 F 32.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  3 F 35.8 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3560 8vs 3 F 35.8 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  4 M 30.2 vs Heart: heart, small 

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, complete  5 M 35.7 vs Head: frontal, right, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th left rib, short  6 M 33.5 vs Girdles: pubis, bilateral, incompletely ossified

 Heart: atrium, bilateral, enlarged  Heart: heart, small  Heart: ventricle, right, small 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 7 F 33.2 vs Heart: heart, small 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3560 8vs 7 F 33.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 8 F 39.9 vs Head: frontal, left, unossified area(s)  Heart: atrium, right, irregular 

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 4th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, right forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Liver: gallbladder, rudimentary  3561 7vs 1 F 45.8 vs Head: anterior supraorbital notch, right, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 2 M 43.1 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified Variants: 13th bilateral rib, complete  3 M 39.4 vs Girdles: clavicle, left, thickened 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 4 M 39.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 5 F 40.1 vs Variants: 13th bilateral rib, complete  6 F 42.6 vs Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3561 7vs 7 M 45.5 vs Variants: 13th bilateral rib, complete  3562 7vs 1 M 35.0 vs Variants: sternebra(e), 5th, unossified 2 M 36.8 vs Heart: atrium, bilateral, distended  3 M 39.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 4 M 40.8 vs Heart: atrium, bilateral, distended  Heart: heart, small 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 5 M 39.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified 6 F 44.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 7 F 43.4 vs Variants: sternebra(e), 5th, unossified 3563 13vs 1 M 44.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 2 F 37.6 vs Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 3 F 30.0 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Thorax: subclavian artery, branching variation, left side 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3563 13vs 4 F 30.4 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 5 F 33.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 6 F 26.3 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 7 M 26.3 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3563 13vs 8 F 35.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, right forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, right hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 9 F 40.4 vs Head: interparietal, supraoccipital, bridge of ossification 

Variants: sternebra(e), 5th, unossified

 10 F 41.9 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 11 F 40.0 vs Head: interparietal, supraoccipital, bridge of ossification 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 12 M 32.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 13 F 41.2 vs Limb bones: metacarpal, 1st digit, bilateral, unossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Thoracic vert.: thoracic vert. centrum(a), 4th-5th, dumbbell ossified

 Variants: 13th right rib, rudimentary 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3565 6vs 1 M 54.2 vs No abnormality detected

 2 F 50.5 vs Variants: sternebra(e), 5th, incompletely ossified

 3 M 50.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

 4 M 49.5 vs Variants: 13th left rib, short 

 5 F 50.5 vs Variants: sternebra(e), 5th, unossified 6 F 54.3 vs Variants: 13th bilateral rib, short  3566 8vs 1 M 38.0 vs THORACIC VERT.: THORACIC HEMICENTRUM(A), 12TH-13TH, RIGHT,  THORACIC VERT.: THORACIC VERTEBRAL ARCH(ES), 11TH, LEFT, SM

 THORACIC VERT.: THORACIC VERTEBRAL ARCH(ES), 8TH-9TH, RIGHT

Head: anterior supraorbital notch, left, incompletely ossified

 Suture/fontanel: parietal suture, sutural bone 

Thoracic vert.: thoracic vert. centrum(a), 11th, asymmetrically ossified

 Thoracic vert.: thoracic vert. centrum(a), 12th, bipartite 

Thoracic vert.: thoracic vert. centrum(a), 8th, asymmetrically ossified

 Thoracic vert.: thoracic vert. centrum(a), 9th, misshapen  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 2 F 33.8 vs Head: parietal, right, unossified area(s) 

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  3 M 33.1 vs Variants: 13th bilateral rib, complete  4 M 34.2 vs Head: anterior supraorbital notch, right, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3566 8vs 4 M 34.2 vs Head: parietal, bilateral, unossified area(s) 

Limb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossified

 Variants: 13th bilateral rib, complete  5 M 31.4 vs Variants: 13th bilateral rib, complete  6 M 30.1 vs Girdles: clavicle, bilateral, bent  Head: hyoid cornua, bilateral, bent outwards  Head: parietal, bilateral, unossified area(s) 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  7 M 35.6 vs Head: anterior supraorbital notch, bilateral, incompletely ossified

 Suture/fontanel: nasal suture, sutural bone  8 M 38.3 vs Heart: heart, small 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 3567 7vs 1 M 47.7 vs Variants: 13th bilateral rib, complete  2 M 44.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified

Variants: sternebra(e), 5th, unossified

 3 F 40.8 vs Head: frontal, right, unossified area(s) 

Limb bones: metacarpal, 1st digit, right, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3567 7vs 3 F 40.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 4 M 47.6 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

 5 F 48.2 vs Cervical: thymus gland, bilateral, partially undescended  Variants: 13th left rib, short  Variants: 13th right rib, rudimentary  6 M 43.1 vs Cervical: thymus gland, bilateral, partially undescended 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 7 F 50.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 3569 11vs 1 F 39.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

 2 M 37.2 vs Variants: 13th left rib, short  Variants: 13th right rib, complete  3 M 35.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 4 F 31.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3569 11vs 5 M 30.5 vs Head: hyoid body, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short, non-articulated 

Variants: sternebra(e), 5th, incompletely ossified

 6 F 23.8 vs Head: hyoid body, unossified

 Head: hyoid cornua, left, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, rudimentary 

Variants: sternebra(e), 6th, unossified

 7 F 32.8 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Thorax: subclavian artery, left side, additional 

 8 M 33.4 vs Lungs: accessory lung lobe, rudimentary  Variants: 13th bilateral rib, rudimentary  9 M 35.9 vs No abnormality detected

 10 M 37.3 vs Girdles: clavicle, left, bent  Suture/fontanel: parietal suture, irregular  11 M 45.2 vs Head: frontal, right, unossified area(s)  3570 9vs 1 M 33.0 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3570 9vs 1 M 33.0 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Liver: gallbladder, rudimentary  Variants: 13th left rib, short 

Variants: sternebra(e), 5th, unossified

 2 F 41.1 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete 

Variants: sternebra(e), 5th, unossified

 3 M 41.9 vs Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossified

 4 F 40.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossified

 5 F 39.5 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  Variants: 13th right rib, rudimentary 

Variants: sternebra(e), 5th, incompletely ossified

 6 F 33.1 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3570 9vs 6 F 33.1 vs Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 7 F 29.6 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 8 M 44.8 vs Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossified

 9 F 38.5 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

 3571 3vs 1 M 46.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral, unossified

 Sternebrae: sternebral center(s), 1st, cranial to, supernumerary ossification 

 2 M 48.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Ribs: distal, 5th, right, nodulated 

Variants: sternebra(e), 6th, incompletely ossified

 3 M 44.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3572 7vs 1 M 36.3 vs Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 2 F 40.1 vs Head: interparietal, incompletely ossified

 Head: parietal, bilateral, fissure(s)  Variants: 13th bilateral rib, complete  3 M 30.0 vs Head: hyoid body, unossified

Head: interparietal, incompletely ossified

 Head: parietal, bilateral, fissure(s) 

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thorax: subclavian artery, left side, additional 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 4 F 34.5 vs Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 5 F 30.4 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 6 M 24.2 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses, unossified

 Variants: 13th left rib, rudimentary, non-articulated 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 7 M 39.1 vs Head: hyoid body, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 3 - 10000 ppm  3572 7vs 7 M 39.1 vs Thorax: subclavian artery, branching variation, left side  Variants: 13th bilateral rib, rudimentary 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4551 11vs 1 M 47.2 vs Head: hyoid cornua, bilateral, bent inwards  2 M 29.8 vs No abnormality detected

 3 M 34.1 vs Lungs: accessory lung lobe, rudimentary  Variants: 13th right rib, short  4 F 37.6 vs No abnormality detected 5 M 32.8 vs No abnormality detected 6 M 31.1 vs Cervical: thymus gland, partially undescended, right side 

Limb bones: epiphyses, incompletely ossified

 Suture/fontanel: frontal suture, plaque of bone  Variants: 13th right rib, complete  7 M 33.1 vs Cervical: thymus gland, partially undescended, right side  Thorax: subclavian artery, left side, additional  Variants: 13th bilateral rib, complete  8 F 38.3 vs Head: parietal, right, unossified area(s)  Variants: 13th bilateral rib, complete  9 M 40.9 vs Variants: sternebra(e), 5th, incompletely ossified

 10 M 42.9 vs Cervical: thymus gland, partially undescended, right side  Variants: 13th bilateral rib, short  11 F 46.4 vs Cervical: thymus gland, bilateral, partially undescended  Lungs: accessory lung lobe, small  Variants: 13th bilateral rib, complete  4552 8vs 1 M 44.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4552 8vs 1 M 44.6 vs Variants: sternebra(e), 6th, unossified 2 M 43.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thorax: subclavian artery, branching variation, left side 

 3 M 42.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short  4 F 43.9 vs Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossified

Limb bones: 2nd phalanx, 4th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  5 M 47.1 vs Cervical: thymus gland, bilateral, partially undescended  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, unossified

 6 F 41.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 7 F 43.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th right rib, short 

 8 F 46.8 vs Cervical: thymus gland, bilateral, partially undescended 

Limb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 6th, incompletely ossified

 4553 8vs 1 M 48.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4553 8vs 2 M 35.6 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: metacarpal, 1st digit, left, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short, non-articulated  Variants: 13th right rib, short  3 M 36.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete  4 M 43.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Suture/fontanel: frontal suture, plaque of bone  Variants: 13th bilateral rib, complete  5 M 45.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  6 M 48.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th left rib, short  7 F 46.9 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Sternebrae: sternebral center(s), 5th-6th, supernumerary ossification, between  Variants: 13th bilateral rib, complete  8 M 47.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4554 9vs 1 M 47.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 2 M 43.4 vs GIRDLES: CLAVICLE, LEFT, BENT 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thorax: subclavian artery, left side, additional  3 M 36.7 vs GIRDLES: CLAVICLE, RIGHT, BENT 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Variants: 13th left rib, rudimentary, non-articulated  Variants: 13th right rib, complete  4 M 41.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete  5 M 37.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short  6 M 38.1 vs GIRDLES: CLAVICLE, RIGHT, BENT 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 7 M 42.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

 8 F 41.9 vs GIRDLES: CLAVICLE, LEFT, ENLARGED 

 GIRDLES: SCAPULA, LEFT, ENLARGED  LIMB BONES: HUMERUS, LEFT, THICKENED 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4554 9vs 8 F 41.9 vs LIMB BONES: METACARPAL, 2ND DIGIT, LEFT, THICKENED  LIMB BONES: RADIUS, LEFT, THICKENED  LIMB BONES: ULNA, LEFT, MISSHAPEN, THICKENED  LIMB BONES: 1ST PHALANX, 2ND DIGIT, 3RD DIGIT, LEFT, THICKENE LUMBAR VERT.: LUMBAR TRANSVERSE PROCESS, 2ND-7TH, LEFT, EN LUMBAR VERT.: LUMBAR TRANSVERSE PROCESS, 5TH-7TH, RIGHT, E Head: parietal, left, unossified area(s)  Paws/limbs: forelimb(s), left, lower, upper, thickened

 Paws/limbs: forepaw(s), left, digit(s), second, misshapen

 Paws/limbs: left forepaw, digit(s), third, misshapen

 Whole body: lumbar spine, musculature, enlarged  9 M 49.4 vs Variants: 13th left rib, rudimentary  4555 9vs 1 F 38.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short 

 2 M 39.0 vs Cervical: thymus gland, bilateral, partially undescended 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Sternebrae: sternebral center(s), 1st, cranial to, supernumerary ossification 

Variants: sternebra(e), 5th, unossified

 3 M 39.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4555 9vs 3 M 39.2 vs Variants: 13th right rib, complete  4 F 37.7 vs Variants: 13th right rib, complete  5 F 30.9 vs Head: parietal, right, fissure(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 6 M 35.4 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

 Variants: 13th bilateral rib, short  7 F 29.0 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 6th, incompletely ossified

 8 M 33.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Liver: gallbladder, rudimentary  Variants: 13th bilateral rib, short  9 F 44.3 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 4556 6vs 1 F 43.5 vs Variants: 13th left rib, complete  Variants: 13th right rib, rudimentary  2 M 47.3 vs Girdles: pelvic girdle, right, unilateral caudal shift  Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4556 6vs 3 F 42.9 vs Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th left rib, complete  Variants: 13th right rib, short  4 F 45.1 vs Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  5 M 46.1 vs Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  6 M 45.2 vs Variants: 13th right rib, short  4557 9vs 1 M 36.1 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 Variants: 13th bilateral rib, short  2 M 35.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 3 M 35.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short  4 F 37.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete  5 F 34.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Thorax: subclavian artery, branching variation, left side  Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4557 9vs 5 F 34.5 vs Variants: sternebra(e), 6th, unossified 6 F 34.5 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thorax: subclavian artery, branching variation, left side  7 M 34.4 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 8 M 38.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 9 M 39.7 vs Head: anterior supraorbital notch, right, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

 4558 9vs 1 M 47.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  2 F 42.0 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  3 F 38.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 4 F 35.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4558 9vs 5 F 30.4 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th right rib, short, non-articulated  6 F 37.5 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

 7 M 45.8 vs Variants: 13th bilateral rib, complete  8 F 39.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified Variants: 13th right rib, short  9 M 49.8 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified 4559 5vs 1 F 50.5 vs Head: premaxilla, left, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short 

 2 F 50.0 vs Suture/fontanel: frontal suture, plaque of bone 

 3 M 54.2 vs No abnormality detected

 4 M 53.4 vs Variants: sternebra(e), 5th, unossified 5 M 56.2 vs Head: hyoid cornua, left, bent outwards 

Variants: sternebra(e), 5th, unossified

 4560 9vs 1 F 43.5 vs Limb bones: metacarpal, 1st digit, left, incompletely ossified

Variants: sternebra(e), 5th, unossified

 2 F 46.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4560 9vs 2 F 46.7 vs Variants: 13th left rib, short 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 3 M 48.0 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 4 F 43.7 vs Head: anterior supraorbital notch, left, incompletely ossified

 Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 5 M 44.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 6 F 37.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, unossified

 7 F 37.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Variants: sternebra(e), 5th, unossified

 8 M 42.8 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4560 9vs 8 M 42.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 9 F 45.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 4561 6vs 1 F 50.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th bilateral rib, complete 

 2 F 45.5 vs Variants: 13th bilateral rib, complete 

 3 M 45.6 vs Variants: 13th bilateral rib, complete 

 4 M 39.8 vs Variants: 13th left rib, complete 

 Variants: 13th right rib, short  5 M 49.2 vs Variants: 13th bilateral rib, complete  6 M 50.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  4562 9vs 1 M 42.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Suture/fontanel: frontal suture, plaque of bone  Variants: 13th left rib, complete  2 M 40.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified 

Variants: sternebra(e), 5th, incompletely ossified 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4562 9vs 2 M 40.2 vs Variants: sternebra(e), 6th, incompletely ossified

 3 M 38.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, rudimentary, non-articulated  4 F 33.9 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Thorax: subclavian artery, branching variation, left side 

 Variants: 13th bilateral rib, complete  5 F 33.4 vs Head: hyoid body, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  6 F 33.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossified

 7 F 35.0 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th bilateral rib, complete  8 M 36.4 vs Head: interparietal, supraoccipital, bridge of ossification 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 9 M 41.2 vs Variants: 13th left rib, complete  Variants: 13th right rib, short, non-articulated  4563 12vs 1 M 43.9 vs Cervical vert.: cervical rib, right, complete with costal cart.  Cervical vert.: cervical rib, left, without costal cartilage  Head: hyoid cornua, left, bent inwards 

Limb bones: 2nd phalanx, 5th digit, left forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4563 12vs 1 M 43.9 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 2 F 38.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, incompletely ossified

 3 M 42.0 vs Head: hyoid cornua, left, bent inwards 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  4 F 39.3 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 4th digit, right hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, unossified

 5 F 35.0 vs HEART: PAPILLARY MUSCLE, RUDIMENTARY  Abdomen: abdominal cavity, fluid filled 

Cervical vert.: odontoid process, incompletely ossified

Head: hyoid body, unossified

 Heart: bilateral ventricles, enlarged 

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, left, unossifiedLimb bones: talus, right, incompletely ossifiedLimb bones: 2nd phalanx, 1st digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 2nd digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4563 12vs 5 F 35.0 vs Limb bones: 2nd phalanx, 2nd digit, bilateral hind limb, unossified

Limb bones: 2nd phalanx, 3rd digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLimb bones: phalanx, 1st digit, right, incompletely ossifiedLimb bones: phalanx, 1st digit, left, unossified

 Liver: all lobes, enlarged  Lungs: lungs (general), rudimentary  Thorax: aorta, distended 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 Whole body: skin/subcutis, edema  6 F 33.3 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Suture/fontanel: frontal suture, plaque of bone  Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4563 12vs 6 F 33.3 vs Variants: sternebra(e), 6th, unossified 7 F 31.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

 8 M 28.6 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossified

 Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, unossified

 9 M 35.1 vs Head: hyoid body, unossified

 Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 10 F 36.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, rudimentary, non-articulated  Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, unossified

 11 M 44.8 vs No abnormality detected

 12 M 43.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, complete 

Variants: sternebra(e), 5th, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4563 12vs 12 M 43.9 vs Variants: sternebra(e), 6th, unossified 4564 10vs 1 M 47.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Suture/fontanel: parietal suture, plaque of bone  Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  2 F 41.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Variants: 13th bilateral rib, complete  3 F 39.9 vs Head: hyoid cornua, bilateral, bent inwards 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th left rib, complete  Variants: 13th right rib, short, non-articulated 

Variants: sternebra(e), 6th, unossified

 4 M 38.2 vs Limb bones: epiphyses, incompletely ossified Variants: 13th bilateral rib, complete  5 M 28.9 vs Limb bones: epiphyses, incompletely ossified Variants: 13th left rib, complete  Variants: 13th right rib, short, non-articulated  6 F 30.6 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4564 10vs 6 F 30.6 vs Limb bones: 2nd phalanx, 5th digit, right forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  7 M 33.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Limb bones: epiphyses, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  8 M 37.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete  9 M 39.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, short, non-articulated  Variants: 13th right rib, complete  10 M 40.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  4565 11vs 1 F 44.0 vs Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 Variants: 13th bilateral rib, complete  2 F 34.7 vs Variants: 13th bilateral rib, complete  3 F 32.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, rudimentary, non-articulated  4 F 26.2 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4565 11vs 4 F 26.2 vs Variants: 13th bilateral rib, complete  5 F 37.7 vs Head: nasal, bilateral, suture within 

Limb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete  6 M 39.1 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, rudimentary, non-articulated  Variants: 13th right rib, complete  7 M 33.5 vs No abnormality detected

 8 F 34.1 vs Variants: 13th bilateral rib, complete  9 F 39.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

 10 F 46.6 vs Variants: 13th bilateral rib, complete  11 F 26.7 vs CAUDAL VERT.: CAUDAL VERTEBRA(E), 14TH-18TH, ABSENT 

 CAUDAL VERT.: CAUDAL VERTEBRA(E), 7TH-11TH, FUSED  LUMBAR VERT.: LUMBAR VERTEBRAL CENTRUM(A), 5TH-6TH, FUSED  Abdomen/trunk: tail, rudimentary

Caudal vert.: caudal vertebra centrum(a), 11th, bipartite ossifiedCaudal vert.: caudal vertebra centrum(a), 1st, bipartite ossifiedCaudal vert.: caudal vertebra(e), 12th-13th, asymmetrically ossified

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4565 11vs 11 F 26.7 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, unossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Liver: gallbladder, absent  Lungs: lungs (general), small  Sternebrae: sternebral center(s), 4th, misaligned  Sternebrae: sternebral center(s), 4th-5th, bridge of ossification  Suture/fontanel: nasal suture, sutural bone  Variants: 13th left rib, complete 

Variants: sternebra(e), 2nd, incompletely ossified

 4566 10vs 1 M 41.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Paws/limbs: forelimb(s), bilateral, flexure(s)

 Paws/limbs: forepaw(s), bilateral, flexure(s)

 Paws/limbs: hindlimb(s), bilateral, flexure(s)

 Sternebrae: sternebral center(s), 2nd-5th, misaligned  Sternebrae: sternebral center(s), 3rd-4th, bridge of ossification  Thorax: subclavian artery, left side, additional  2 M 47.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Paws/limbs: forelimb(s), bilateral, flexure(s)

 Paws/limbs: forepaw(s), bilateral, flexure(s)

 Paws/limbs: hindlimb(s), bilateral, flexure(s)

 Thorax: thoracic cavity, fluid filled  Variants: 13th left rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4566 10vs 2 M 47.9 vs Variants: 13th right rib, short  3 F 42.0 vs Head: frontal, right, fissure(s)  Variants: 13th bilateral rib, complete  4 F 43.0 vs Thorax: subclavian artery, branching variation, left side  5 M 33.3 vs Variants: 13th right rib, short  6 F 27.2 vs Paws/limbs: forelimb(s), bilateral, flexure(s)

 Paws/limbs: hindlimb(s), bilateral, flexure(s)

 Variants: 13th bilateral rib, complete  7 M 40.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Paws/limbs: forelimb(s), bilateral, flexure(s)

 Paws/limbs: forepaw(s), bilateral, flexure(s)

 Paws/limbs: hindlimb(s), bilateral, flexure(s)

 Suture/fontanel: parietal suture, enlarged  Variants: 13th right rib, short  8 F 33.7 vs Paws/limbs: hindlimb(s), bilateral, flexure(s) Variants: 13th left rib, complete  Variants: 13th right rib, rudimentary  9 M 37.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossified

 10 M 42.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th bilateral rib, short 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4567 10vs 1 F 37.9 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossified

 2 F 36.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present  Variants: 13th left rib, complete 

Variants: sternebra(e), 5th, unossified

 3 F 44.8 vs Variants: 13th right rib, short  4 F 42.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  5 M 38.9 vs Variants: 13th right rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 6 F 43.8 vs No abnormality detected

 7 F 37.1 vs Variants: 13th bilateral rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 8 F 28.4 vs Suture/fontanel: anterior fontanelle, enlarged  Variants: 13th left rib, complete  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 9 F 38.5 vs Limb bones: epiphyses, incompletely ossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4567 10vs 10 F 45.3 vs Variants: sternebra(e), 5th, incompletely ossified

 4568 11vs 1 M 49.9 vs Abdomen: jejunum, cyst  Cervical: thymus gland, partially undescended, right side 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Sternebrae: sternebral center(s), 3rd-4th, bridge of ossification  Sternebrae: sternebral center(s), 4th, misaligned  Sternebrae: sternebral center(s), 4th-5th, fused  Suture/fontanel: anterior fontanelle, enlarged 

 Thorax: subclavian artery, left side, additional 

 Variants: 13th left rib, short  Variants: 13th right rib, complete  2 F 47.4 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified Variants: 13th right rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 3 M 40.7 vs Heart: ventricle, av-valve, right, nodule 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Variants: 13th left rib, complete  4 F 32.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th left rib, short  Variants: 13th right rib, complete  5 F 33.9 vs Head: frontal, left, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4568 11vs 5 F 33.9 vs Variants: 13th bilateral rib, short  6 F 30.0 vs Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 7 M 34.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th right rib, short  8 F 37.7 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th right rib, short  9 M 44.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Thorax: subclavian artery, branching variation, left side  Variants: 13th left rib, short, non-articulated  Variants: 13th right rib, complete  10 M 44.4 vs Variants: sternebra(e), 5th, unossified 11 F 42.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th bilateral rib, complete  4569 pd 10vs 1 F 42.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

 Thorax: subclavian artery, left side, additional 

 Variants: 13th left rib, short  2 M 33.3 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: metacarpal, 1st digit, left, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, short pd = premature delivery, excluded from summary 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4569 pd 10vs 2 M 33.3 vs Variants: 13th right rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 3 F 21.6 vs Head: hyoid body, unossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 1st digit, left forelimb, incompletely ossifiedLimb bones: epiphyses, unossified

 Suture/fontanel: anterior fontanelle, enlarged  Thorax: subclavian artery, left side, additional 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 4 F 35.0 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Lungs: accessory lung lobe, absent  Variants: 13th left rib, rudimentary  5 F 39.0 vs Head: frontal, right, unossified area(s) 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 6 F 24.8 vs Limb bones: metacarpal, 1st digit, right, incompletely ossified

Limb bones: metacarpal, 1st digit, left, unossifiedLimb bones: talus, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

pd = premature delivery, excluded from summary 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4569 pd 10vs 6 F 24.8 vs Limb bones: epiphyses, unossified

 Variants: 13th left rib, short 

Variants: sternebra(e), 5th, incompletely ossified

 7 F 30.4 vs Limb bones: epiphyses, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 8 F 21.3 vs Cervical vert.: odontoid process, incompletely ossified

Head: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 9 M 29.8 vs Cervical vert.: odontoid process, incompletely ossified

Limb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: epiphyses, unossified

Variants: sternebra(e), 6th, unossified

 10 M 29.1 vs Head: interparietal, supraoccipital, bridge of ossification 

Limb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 6th, unossified

pd = premature delivery, excluded from summary 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4570 6vs 1 M 51.8 vs Variants: sternebra(e), 5th, unossified 2 F 42.8 vs Girdles: clavicle, left, bent 

Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified

 Variants: 13th left rib, short  Variants: 13th right rib, rudimentary, non-articulated 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified

 3 M 45.7 vs Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 4 F 39.0 vs Girdles: clavicle, right, bent 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Variants: sternebra(e), 5th, unossified

 5 F 45.8 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 6 F 41.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 4571 8vs 1 F 47.5 vs Thorax: subclavian artery, left side, additional  Variants: 13th bilateral rib, short  2 M 45.8 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Thorax: subclavian artery, left side, additional 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4571 8vs 3 F 42.7 vs Limb bones: metacarpal, 1st digit, bilateral, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  4 F 40.6 vs Lungs: accessory lung lobe, absent  Thorax: subclavian artery, left side, additional  Variants: 13th left rib, complete  Variants: 13th right rib, short  5 M 37.7 vs Cervical: thymus gland, partially undescended, left side 

Limb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedLimb bones: epiphyses, incompletely ossified

 Variants: 13th left rib, complete  Variants: 13th right rib, short  6 F 40.5 vs Thorax: subclavian artery, left side, additional  7 M 43.3 vs Variants: 13th left rib, short  8 F 44.4 vs No abnormality detected 

 4572 10vs 1 M 45.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: frontal suture, plaque of bone  Suture/fontanel: parietal suture, plaque of bone  Variants: 13th left rib, short  Variants: 13th right rib, complete  2 F 41.9 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Suture/fontanel: frontal suture, plaque of bone 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4572 10vs 2 F 41.9 vs Variants: 13th bilateral rib, complete  3 M 36.3 vs Head: interparietal, supraoccipital, fused 

Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Suture/fontanel: frontal suture, plaque of bone  Variants: 13th left rib, short, non-articulated  Variants: 13th right rib, short 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 4 F 35.6 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

Variants: sternebra(e), 6th, incompletely ossified

 5 F 32.3 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified

 6 M 32.1 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

Limb bones: epiphyses, incompletely ossified

 Suture/fontanel: frontal suture, plaque of bone  Variants: 13th left rib, short  Variants: 13th right rib, complete  7 F 34.3 vs GIRDLES: CLAVICLE, BILATERAL, BENT 

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Thoracic vert.: 20 thoracolumbar vertebrae, present 

SANITIZED
Huntingdon Life Sciences 09-4348 

 MA-252E-93-23 

Individual Fetal Observations 

Animal Weight Examination Findings 

Number Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification in italics

Group 4 - 15000 ppm  4572 10vs 7 F 34.3 vs Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 6th, incompletely ossified

 8 M 36.9 vs Head: hyoid cornua, right, bent inwards  Head: interparietal, supraoccipital, fused 

Limb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified

 Variants: 13th bilateral rib, complete 

Variants: sternebra(e), 5th, incompletely ossified

 9 M 40.2 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified

 Variants: 13th bilateral rib, short, non-articulated  10 M 43.5 vs Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossified Variants: 13th bilateral rib, complete 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 284 

 MA-252E-93-23 Final Report

 

Equipment List Appendix M 

Exposure Chamber 

1500 Liter glass and stainless steel chamber (Unifab) 

Compound Generator 

Counter-Current Volatilization Unit, with a coiled glass rod insert (Crown Glass Co., Inc.) 

heated by a nichrome wire (Crown Glass Co., Inc.) monitored with a Big Digit 

Humidity/Temperature Meter (VWR) 

Compound Reservoir

Sponsor Cylinder 

Variable Auto Transformer

Variable Autotransformer, Type 3PN 1010 (Staco Energy Products Company) 

Flowmeters 

Liquid flowmeter, size 0-65 mm, Tube No. 6G03-R3, 6G04-R3 (Key Instruments) 

Flowmeter, size 0 – 4, 0 – 5, 0 – 10, 0 – 20, 0 - 30 or 0 - 40 Lpm (Dwyer®) 

Pressure/Vacuum Gauge(s)

Norgreen backpressure gauge, P/N R07-219-RGKA 

U.S. Gauge backpressure gauge 

Regulator(s)

Norgreen, P/N R07-219-RGKA 

Valves

Metering Valve, Model SS-4L Series (Nupro® Co.) 

Toggle Valve 

Tubing

Size 1/4”, 1/2”, 3/16”, PVC type, plastic (Masterkleer) 

Teflon®, size 1/8”, 1/4” 

1/2” stainless steel 

T-Tube, stainless steel 

Filters

Balston® Microfibre Disposable Filter Units (Grade DQ) 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 285 

 MA-252E-93-23 Final Report

 

Equipment List Appendix M 

Vacuum Pumps

Thomas Industries Inc., Model 707CM50 

Balance

Pelouze 4040 

Air Analyzer

MIRAN® 1A-CVF Ambient Air Analyzer (Wilks) with a Cole Parmer strip chart 

recorder, Model 201, and a Micronta® LCD Benchtop Digital Multimeter No. 22-195 

Particle Sizer/Analyzer

TSI Aerodynamic Particle Sizer, Model 331001, with a DELL computer, Model 486P/25, 

equipped with an Epson LQ-570+ Dot matrix printer, Model P630B 

Environmental Monitoring

Springfield® Apollo Temperature and Humidity Gauge, calibrated with a Digital 

Hygrometer and Thermometer, Model 11-661-18 (Fisher Scientific) 

Chamber Air-flow Gauge(s)

Dwyer® Magnahelic gauge, calibrated prestudy with Dry Gas Meter, Model 2M (Singer) 

Chamber Static Pressure

Dwyer® Magnehelic® gauge, calibrated prestudy with Dwyer Mark II Manometer, 

Model 25 (Dwyer

SANITIZED

 Instruments Inc.)
Huntingdon Life Sciences 09-4348 Page 286 

 MA-252E-93-23 Final Report

 

Pre-Study Chamber Distribution Samples Appendix N 

Group 

(target ppm) Date Port 

Concentration 

(ppm) Ratio to H-1 

2 (2500) 10 Mar 10 H-1 2500 - 

 H-2 2200 0.88 

 H-3 2300 0.92 

 H-4 2400 0.96 

 H-7 2000 0.80 

 H-8 2100 0.84 

 H-1 2400 - 

 H-9 1600 0.67 

 H-9 1500 0.63 

 H-10 1800 0.75 

 H-1 2700 - 

 H-12 3000 1.11 

 H-11 2700 1.00 

 H-14 2300 0.85 

 H-13 2000 0.74 

 11 Mar 10 H-1 2400 - 

 H-7 2000 0.83 

 H-8 2200 0.92 

 H-9 1900 0.79 

 H-1 2300 - 

 H-10 2400 1.04 

 

3 (10000) 10 Mar 10 H-1 10000 - 

 H-2 9300 0.93 

 H-3 9600 0.96 

 H-4 9200 0.92 

 H-1 10000 - 

 H-7 9300 0.93 

 H-8 9300 0.93 

 H-9 9700 0.97 

 H-10 10000 1.00 

 H-1 10000 - 

 H-14 10000 1.00 

 H-13 10000 1.00 

 H-12 10000 1.00 

 H-11 9800 0.98 

 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 287 

 MA-252E-93-23 Final Report

 

Pre-Study Chamber Distribution Samples Appendix N 

Group 

(target ppm) Date Port 

Concentration 

(ppm) Ratio to H-1 

4 (15000) 10 Mar 10 H-1 14000 - 

 H-2 14000 1.00 

 H-3 14000 1.00 

 H-4 14000 1.00 

 H-7 14000 1.00 

 H-1 14000 - 

 H-8 13000 0.93 

 H-9 14000 1.00 

 H-10 14000 1.00 

 H-12 14000 1.00 

 H-1 14000 - 

 H-11 14000 1.00 

 H-14 14000 1.00 

 H-13 14000 1.00 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 288 

 MA-252E-93-23 Final Report 

 

Miran® Calibration Appendix O 

Methodology for 

Settings: The instrument settings for the unit are summarized below: 

 wavelength, microns 3.26 

 pathlength, dial setting 14.00 

 slit width, mm 1 

 range, absorbance 1 

 response, seconds 1 

 gain high 

 chart speed, cm/min 1 

 chart volts 1 

Calibrations: The Miran® was turned on and allowed to warm up for approximately 

10 minutes. The cell was flushed with room air for approximately one minute. The loop 

was closed, the unit was zeroed and the calibration series was performed as shown below. 

The resultant data were plotted to obtain a calibration curve. Each observer used a 

separate syringe for calibration. 

Injection Calculated Absorbance 

Volume Concentration1

 Operator 1 Operator 2 Average 

(μL of liquid) (ppm) (volts) (volts) (volts) 

40 1693 0.315 0.2881 0.302 

60 2539 0.343 0.354 0.349 

240 10156 0.605 0.588 0.597 

360 15234 0.771 0.724 0.748 

430 18197 0.877 0.803 0.840 

1

Calculated Conc. (ppm): Volume injected (μL) X 1.3 mg/μL X 1000 μg/mg ÷ 5.64 L ÷ 130.5 μg/μmole 

X 22.4 μL/μmole X (292 K/273 K) 

 where: 5.64 L = volume of Miran closed loop 

 1.3 mg/μL = density of test substance 

 130.5 μg/μmole = molecular weight of test substance 

Syringes: 0-500 μL Hamilton gas-tight syringe, Model No. 1750 was used for injections. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 289 

 MA-252E-93-23 Final Report 

 

Miran® Calibration Appendix O 

Calibration Checks: A three-point calibration check of the Miran® was performed for 

each exposure prior to sampling the chambers. The parameters are shown below: 

 Expected Acceptable 

Injection Calculated Absorbance Absorbance 

Volume Concentration Reading Range 

(μL of liquid) (ppm) (volts) (volts) 

 

60 2539 0.349 0.2967 – 0.401 

240 10156 0.597 0.507 – 0.687 

360 15234 0.748 0.636 – 0.860 

The absorbance was recorded after each injection. The absorbance was considered 

satisfactory if it was within 15% of the original calibration series. If any of the 

absorbance values fell outside the 15% range, the injection was rechecked as follows. 

The volume for the value that was out of range was reinjected twice. The closer pair of 

the three injections were averaged and the results were compared to the original curve. If 

the average of the pair was within the 15% range, the original was accepted. If the value 

of the average was outside the 15% range, the Study Director decided if a new graph was 

to be prepared. 

SANITIZED
Huntingdon Life Sciences 09-4348 Page 290 

 MA-252E-93-23 Final Report 

 

Miran® Calibration Appendix O 

Calibration Curve for 

y = -50119x3 + 87520x2 - 16855x

R2

 = 0.992

0

2000

4000

6000

8000

10000

12000

14000

16000

18000

20000

0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.900 1.000

Absorbance (Volts)

Concentration (ppm)

SANITIZED
Appendix P Page 291

SANITIZED
Appendix P Page 292

SANITIZED
Appendix P Page 293

SANITIZED
Appendix P Page 294

SANITIZED
Appendix P Page 295

SANITIZED
Appendix P Page 296

SANITIZED
Appendix P Page 297

SANITIZED
Huntingdon Life Sciences 09-4348 Page 298 

 MA-252E-93-23 Final Report 

 

Scientists and Supervising Personnel Appendix Q 

TITLE/DEPARTMENT NAME/DEGREE 

SENIOR VICE PRESIDENT, 

 SAFETY ASSESSMENT 

Sylvie J Gosselin, DVM, PhD, 

 Diplomate ACVP 

 

DIRECTOR, DEVELOPMENTAL AND 

 REPRODUCTIVE TOXICOLOGY Robert M Parker, PhD, DABT 

 

DIRECTOR, ANALYTICAL SERVICES Barbara A Litzenberger, BS, 

 MT (ASCP) 

 

DIRECTOR, TOXICOLOGY 

 OPERATIONS Ian Vanterpool, FIAT 

 

STUDY DIRECTOR Gary M Hoffman, BA, DABT 

REPORT WRITER Ronald L. Brzozowski, BA, 

 AALAS LAT 

 

VETERINARIAN Teresa S Kusznir, VMD 

MANAGER/SUPERVISOR 

 Rodent Toxicology 

 Inhalation Toxicology 

 Pharmacy 

 Necropsy and Fetal Pathology 

Christina Savidge, BS, 

 AALAS RLATG 

Stuart Cracknell, CBiol, MIBiol 

Michael S McCarthy 

Donna G Chuddley, BS, 

 CLA (ASCP) HT 

 

SANITIZED