# Data Requirement

EPA OPPTS 870.3700

OECD 414

HLS STUDY NO. 09-4348

SPONSOR STUDY NO. MA-252E-93-23

EMBRYO-FETAL TOXICITY STUDY IN RABBITS

VIA WHOLE-BODY INHALATION EXPOSURE (GLP)

Final Report

Author: Gary M. Hoffman, BA, DABT

Performed by: Huntingdon Life Sciences

100 Mettlers Road

East Millstone, New Jersey 08875-2360

Submitted to:

Attn:

Date: 2 September 2010

Page 1 of 298

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LIST OF ABBREVIATIONS

Abbreviation Term

F Female(s)

GD or GES Gestation Day

hr(s) hour(s)

Lpm Liters per minute

mg/L milligrams of test substance per Liter of air

min(s) minute(s)

NOAEL No-Observed-Adverse-Effect-Level

NOEL No-Observed-Effect-Level

np non-pregnant

ppm parts per million

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Embryo-Fetal Toxicity Study in Rabbits

via Whole-Body Inhalation Exposure (GLP)

SUMMARY

The objective of this study was to assess any gr oss maternal and/or embryo-fetal toxicity

of in a non-rodent species , the rabbit, following whole-body inhalation

exposure (every day from Gestation Day ( GD) 6 to GD 28, inclusively), covering the

period of major organogenesis (which extend s from implantation to the day prior to

expected delivery, approximately GD 6 to GD 28).

Eighty-eight timed-mated female New Zeala nd White rabbits were distributed into

4 groups (1 control and 3 treated groups) of 22 dams/group. These animals received

whole-body inhalation exposures every day with clean air (air control) or with

at exposure concentrations of 2500, 10000 or 15000 ppm from GD 6 to GD 28

(GD 0 = day of mating confirmation). The exposure durations were 6 hours per day for

7 days per week.

All animals were euthanized and necropsie d on GD 29. The following parameters were

evaluated for all animals: viability, c linical observations, body weights and feed

consumption. A macroscopic postmortem evaluation was performed on GD 29, during

which corpora lutea and implantation site s were counted and uterine and placental

weights were recorded. Fetuses were rem oved, weighed (live fetuses only), sexed and

examined externally for defects, for soft tissue abnormalities and for skeletal

abnormalities and ossification state.

The target and mean analytical (IR) and nominal concentrations are summarized as follows:

Group Target

Concentration

(ppm) Analytical

Concentration

(ppm) Nominal

Concentration

(ppm)

1 0 0.0 0

2 2500 2513 NA

3 10000 9945 NA

4 15000 14942 30154*

*the combined nominal concentration for all 3 exposed groups

There were no effects of exposures on su rvival, clinical signs, body weights, food

consumption or maternal or fetal findings at sacrifice. Therefore, the no-observed-effect

level (NOEL) for adult pregnant female rabbits for systemic and reproductive and

developmental toxicity was 15,000 ppm of

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TABLE OF CONTENTS

COVER PAGE ....................................................................................................................

. 1

STATEMENT OF COMPLIANCE ..................................................................................... 2

SIGNATURE PAGE ........................................................................................................... 3

QUALITY ASSURANCE STATEMENT .......................................................................... 4

LIST OF ABBREVIATIONS .............................................................................................. 5

SUMMARY .......................................................................................................................

.. 6

TABLE OF CONTENTS ..................................................................................................... 7

1. 

# INTRODUCTION

................................................................................................ 10

2. 

# MATERIALS AND METHODS

2.1. Study Management .................................................................................... 10

2.2. Study Dates ................................................................................................ 10

2.3. Experimental Outline ................................................................................. 11

2.4. Justifications .............................................................................................. 11

2.5. Test Substance ........................................................................................... 13

2.6. Test Animals .............................................................................................. 15

2.7. Animal Assignment ................................................................................... 15

2.8. Animal Identification ................................................................................. 16

2.9. Veterinary Care .......................................................................................... 16

2.10. Husbandry During Non-Exposure Periods ................................................ 16

2.11. Husbandry During Exposure Periods ........................................................ 18

2.12. Test Substance Administration and Chamber Operation ........................... 19

2.13. Experimental Ev aluations .......................................................................... 23

2.14. Terminal Observations ............................................................................... 23

2.15. Statistical An alysis ..................................................................................... 25

2.16. Major Computer Systems .......................................................................... 28

2.17. Data Storage ............................................................................................... 28

2.18. Regulatory References ............................................................................... 29

2.19. Protocol Deviation ..................................................................................... 29

3. 

# RESULTS AND DISCUSSION

3.1. Chamber Monitoring .................................................................................. 30

3.2. Maternal Data ............................................................................................ 31

3.3. Fetal Data ................................................................................................... 32

4. 

# CONCLUSION

.................................................................................................... 33

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TABLE OF CONTENTS

REFERENCES .................................................................................................................. 34

CALCULATIONS ............................................................................................................. 35

GENERAL PREFACE ...................................................................................................... 36

FIGURES

1. Diagram of Generation System and Exposure Chamber - Group 1 ............................. 37

Diagram of Generation System and Exposure Chamber - Groups 2-4 ........................ 38

2. Mean Body Weights .................................................................................................... 39

3. Mean Food Consumption Values................................................................................. 40

TABLES

1. Summary of Survival ................................................................................................... 41

2. Summary of In-Chamber Observations ....................................................................... 42

3. Summary of Daily Observations .................................................................................. 44

4. Summary of Physical Examination Findings ............................................................... 48

5. Mean Body Weights .................................................................................................... 53

6. Mean Body Weight Changes ....................................................................................... 54

7. Mean Food Consumption Values................................................................................. 55

8. Incidence Summary Report for Gross Necropsy Observations ................................... 56

9. Summary of Pregnancy ................................................................................................ 58

10. Summary of Cesarean Section Data ............................................................................ 59

11. Summary of Gravid Uterine Adjusted Body Weights ................................................. 60

12. Mean Litter Weights .................................................................................................... 61

13. Summary of Fetal Observations ................................................................................... 62

External ......................................................................................................................

.. 63

Visceral ......................................................................................................................

.. 64

Skeletal ......................................................................................................................

... 67

Ossification Parameters ............................................................................................... 71

APPENDICES

A. Chamber Monitori ng Results ....................................................................................... 74

B. Individual Animal Term ination History ...................................................................... 82

C. Individual Daily Ob servations ..................................................................................... 86

D. Individual Physical Ex amination Findings .................................................................. 93

E. Individual Body Weights ........................................................................................... 114

F. Individual Body Weight Changes .............................................................................. 122

G. Individual Food Consum ption Values ....................................................................... 130

H. Raw Data Listing of Gross Obse rvations with Modifiers .......................................... 138

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TABLE OF CONTENTS

I. Individual Cesarean Section Data .............................................................................. 144

J. Individual Gravid Uterine Adjusted Body Weights .................................................. 152

K. Individual Litter We ight Data .................................................................................... 160

Individual Litter Fetal a nd Placental Weights ........................................................... 161

Individual Feta l Weights ............................................................................................ 169

Individual Placenta l Weights ..................................................................................... 177

L. Individual Fetal Ob servations .................................................................................... 185

M. Equipment List ........................................................................................................... 284

N. Pre-study Chamber Dist ribution Samples .................................................................. 286

O. Miran  

# Calibration ..................................................................................................... 288

P. Certificates of Analysis .............................................................................................. 291

Q. Scientists and Supe rvising Personnel ........................................................................ 298

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1. INTRODUCTION

The purpose of this study was to assess gross maternal and/or embryo-fetal

toxicity of in a non-r odent species, the ra bbit, following whole

body inhalation exposure (every day from Gestation Day (GD) 6 to GD 28,

inclusively), covering the period of ma jor organogenesis (which extends from

implantation to the day prior to expected delivery, approximately GD 6 to

GD 28).

2. MATERIALS AND METHODS

2.1. STUDY MANAGEMENT

2.1.1. SPONSOR

2.1.2. SPONSOR REPRESENTATIVE

2.1.3. TESTING FACILITY

Huntingdon Life Sciences

100 Mettlers Road

East Millstone, New Jersey 08875-2360

2.1.4. STUDY DIRECTOR

Gary M. Hoffman, BA, DABT

2.2. STUDY DATES

2.2.1. STUDY INITIATION

17 February 2010 (Date Study Dire ctor signed the Protocol)

2.2.2. ANIMAL RECEIPT

10 and 12 March 2010 (Experimental Start Date)

2.2.3. EXPOSURE INITIATION

14, 15, 16 or 17 March 2010

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2.2.4. EXPOSURE TERMINATION

5, 6, 7 or 8 April 2010

2.2.5. TERMINAL SACRIFICE

6, 7, 8 or 9 April 2010

2.2.6. EXPERIMENTAL COMPLETION

26 May 2010

2.2.7. STUDY COMPLETION

2 September 2010 (Date Study Direct or signed the Final Report)

2.3. EXPERIMENTAL OUTLINE

Four (4) experimental groups (1 c ontrol and 3 treated groups) received

whole-body inhalation exposures every da y with clean air or with

at the exposure concentrations summarized below. The

animals were exposed every day from Gestation Day (GD) 6 to GD 28

(GD 0 = day of mating). Each group consisted of 22 time-mated females

as outlined below:

Exposure Levels a 

# Test Animals (females)

Group Group

Designation (ppm) (mg/L) Total on

Study Necropsy

1 Air Control 0 0 22 22

2 Low 2500 13 22 22

3 Mid 10000 53 22 22

4 High 15000 80 22 22

a 

# Exposures were 6 hours per day for 7 consecutive days per week. Exposure levels are

expressed as ppm and equivalent mg/L of test substance; results are reported in ppm.

The exposures were conducted via whole-body exposure.



# 2.4. JUSTIFICATIONS

2.4.1. ROUTE, DURATION AND FREQUENCY OF

ADMINISTRATION

The inhalation route is one of the potential routes of human

exposure to this test article. The duration and frequency of

exposures were as recommended in the relevant test guideline,

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ensuring adequate exposure of post-implantation embryo-fetal

development.

2.4.2. EXPOSURE LEVEL SELECTION

A pilot 4-week inhalation study was performed with male and

female CD rats exposed to target levels of 0, 2000, 4500, 7500 and

10000 ppm of Effects were limited to mild

reversible alterations in some hematology parameters at

10000 ppm and to a lesser extent at 7500 ppm. Based on the above

information, a high exposure level of 15000 ppm was selected for a

range-find rabbit inhalation st udy (Huntingdon Life Sciences

Study No. 09-4347) to provide slight maternal toxicity while lower

levels of 10000 and 2500 ppm were selected to provide a range of

possible effects including a NOEL or NOAEL. The range-find

rabbit inhalation study showed no eff ect at all exposure levels and

therefore the same exposure levels were selected for this study.

2.4.3. TEST ANIMAL SELECTION

The rabbit is accepted by Regulatory Authorities as a surrogate for

humans in the detection of effect s on embryo-fetal development. It

is the preferred non-rodent species for developmental toxicity

testing, for practical reasons and in view of the large amount of

accumulated background information in the species. Also a rangefind rabbit inhalation study (Huntingdon Life Sciences Study

No. 09-4347) was conducted with this test substance in preparation

for this definitive study.

2.4.4. NUMBER OF ANIMALS

The number of animals in this study (22 time-mated

females/group) was considered the minimum necessary to allow

for meaningful interpretation of the outcome and was based on the

following key concepts:

• The OECD and EPA guidelines i ndicate that no less than

16 pregnancies/group be evaluated. Some of the endpoints of

principal interest (e.g., embr yonic death, malformation) are

low-frequency events.

• A reduction in litters available for evaluations is possible due

to the following events:

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Low pregnancy rate. The normally expected pregnancy rate in

this strain from our curr ent vendors is approximately

85 to 95%.

Unexpected deaths and/or abortions.

A number of 22 time-mated females provides a sufficient

number of pregnancies for ev aluation in each group (as it

allows for ≥ 16 pregnancies/group, even if half the potentially

not pregnant females are assign ed to a particular group).

Further to this, 22 rabbits per group reduces the risk of having

to repeat the study due to an insufficient number of litters for

evaluation.

At least three exposure levels ar e required by regulatory agencies

to describe an exposure relations hip in any effects observed, in

comparison with an air control group.

2.5. TEST SUBSTANCE

2.5.1. TEST SUBSTANCE CATEGORY

Industrial chemical used as a po ssible refrigerant and solvent.

2.5.2. MANUFACTURER/SUPPLIER

2.5.3. LOT NUMBERS

41024-38

KP-4

BZ056A-1

BZ056A-2

BZ056A-3

BZ056A-4

BZ056B-1

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2.5.4. PURITY

Approximately 100% as per Ce rtificates of Analysis

2.5.5. DESCRIPTION

Liquid in cylinder

2.5.6. DATES RECEIVED

41024-38: 18 May 2009

KP-4: 17 June 2009

BZ056A-1: 2 March 2010

BZ056A-2: 2 March 2010

BZ056A-3: 2 March 2010

BZ056A-4: 2 March 2010

BZ056B-1: 17 March 2010

2.5.7. EXPIRATION DATES

41024-38: 13 May 2010

KP-4: 15 June 2010

BZ056A-1: 3 March 2011

BZ056A-2: 3 March 2011

BZ056A-3: 3 March 2011

BZ056A-4: 3 March 2011

BZ056B-1: 3 March 2011

2.5.8. ANALYSIS

Documentation of the identity, strength, purity, composition,

stability, and method of synthesis, fabrication, and/or derivation of

the test substance and the maintenance of these records were the

responsibility of the Sponsor. Ce rtificates of Analysis were

provided by the Sponsor and ar e included in Appendix P

.

2.5.9. STORAGE

Room temperature, protected from light

2.5.10. ARCHIVAL SAMPLE

A sample from each lot of test substance is stored in the Archives

of the Testing Facility.

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2.5.11. DISPOSITION

The unused portion of the test substance and any empty test

substance containers were return ed to the Sponsor on 12 July 2010.

2.6. TEST ANIMALS

2.6.1. SPECIES

Albino Rabbits

New Zealand White

2.6.2. SUPPLIER

Covance Research Products

Denver, Pennsylvania

2.6.3. NUMBER OF ANIMALS

Received/Placed on test:

88 timed-mated females

2.6.4. AGE AT RECEIPT

Approximately 5 to 6 months at receipt on GD 1 or 2, where GD 0

is the day of mating.

2.6.5. AGE AT INITIATION OF EXPOSURE

Approximately 5 to 6 months old.

2.6.6. WEIGHT AT INITIATION OF EXPOSURE (KILOGRAMS)

Mean Range

3.4 2.9 to 4.1

2.6.7. STABILIZATION PERIOD

The stabilization period was restrict ed to approximately 4 to 5 days

prior to commencement of exposur es, as dictated by the mating

and delivery schedule for the animals.

2.7. ANIMAL ASSIGNMENT

The exact number of animals required for the study were purchased and

stabilized. Animals were randomly assigned to control or treated groups

upon arrival (4 groups of 22 animals/group). Prior to exposures, the body

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weights obtained on GD 4 were reviewed to ensure minimization of intergroup variability based on those body weights.

2.8. ANIMAL IDENTIFICATION

Each animal was identified with an ear-tag bearing its assigned animal

number. The assigned animal numb er plus the study number comprised

the unique animal number for each animal. In addition, each cage was

provided with a cage card which was color-coded fo r exposure level

identification and contained stu dy number and animal number

information.

2.9. VETERINARY CARE

Animals were monitored by the technical staff for any conditions requiring

possible veterinary care a nd treated as necessary. Miscellaneous, non-test

article-related veterinari an evaluations and treatments (as necessary) for

individual animals were review ed by the study director and are

documented in the study file.

2.10. HUSBANDRY DURING NON-EXPOSURE PERIODS

2.10.1. FACILITIES MANAGEMENT/ANIMAL HUSBANDRY

Currently acceptable practices of good animal husbandry were

followed e.g., Guide for the Care and Use of Laboratory Animals ;

National Academy Press, 1996. Huntingdon Life Sciences, East

Millstone, New Jersey is fully accredited by the Association for

Assessment and Accreditation of Laboratory Animal Care

International (AAALAC).

2.10.2. HOUSING

All animals were housed individual ly in stainless steel suspended

cages with grid bottoms. A stainless steel food hopper was

attached to each cage.

2.10.3. FEED

Global High Fiber Diet #2031C (Harlan Teklad, Madison,

Wisconsin) was available in m easured amounts, up to 200 grams

per day. Fresh feed was presented daily. Feed was presented at

approximately the same time each day.

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2.10.4. FEED ANALYSIS

Analysis of each feed lot used du ring this study was performed by

the manufacturer. Results were provided to the Testing Facility

and are maintained on file at the Testing Facility. There were no

known contaminants in the feed th at were expected to interfere

with the results of this study.

2.10.5. WATER

Water (Elizabethtown Water Company, Westfield, New Jersey)

was available without restriction via an automated watering

system.

2.10.6. WATER ANALYSIS

Water analyses are conducted by Elizabethtown Water Company,

Westfield, New Jersey (Raritan-Millstone Plant) to ensure that

water meets standards specified under the EPA Federal Safe

Drinking Water Act Regulations (40 CFR Part 141). In addition,

water samples are collected biannually from representative rooms

in the Testing Facility; chemical and microbiological water

analyses are conducted on these samples by a subcontract

laboratory. Results of all water analyses are maintained on file at

the Testing Facility. There were no known contaminants in the

water which were expected to inte rfere with the results of this

study.

2.10.7. ENVIRONMENTAL CONDITIONS

Light/Dark Cycle

A twelve-hour light/dark cycle controlled via an automatic timer

was provided.

Temperature

Temperature was monitored in accordance with Testing Facility

SOPs and maintained within the specified range to the maximum

extent possible.

Desired Range: 16 to 22 ° C

Daily Average Range: 18 to 20 ° C

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Relative Humidity

Relative humidity was monitored in accordance with Testing

Facility SOPs and maintained within the specified range to the

maximum extent possible.

Desired Range: 30 to 70%

Daily Average Range: 39 to 68%

Air Changes

The number of air changes in the animal rooms is maintained at 10

to 15 air changes per hour and monitored at least twice each year

in accordance with Testing Facility SOPs.

2.10.8. ENRICHMENT

Animals were provided with enrich ment devices (toys and/or feed

treats) in accordance with Testing Facility SOPs.

2.11. HUSBANDRY DURING EXPOSURE PERIODS

Animals were individually housed in su spended, stainless steel wire mesh

cages.

2.11.1. FEED

None was provided during exposure.

2.11.2. WATER

None was provided during exposure.

2.11.3. ENVIRONMENTAL CONDITIONS

Chamber temperature and relative humidity were recorded every

half-hour during exposure and main tained, to the maximum extent

possible, within the ranges presented below. Excursions outside

the specified range did not affect the integrity of the study.

Temperature

Desired: 16 to 22 ° C

Actual: 17 to 23 ° C

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Relative Humidity

Desired: 40 to 60%

Actual: 47 to 66%

2.12. TEST SUBSTANCE ADMINISTRATION AND

CHAMBER OPERATION

2.12.1. ROUTE OF ADMINISTRATION

Inhalation as a vapor by whole-body exposure.

2.12.2. FREQUENCY AND DURATION OF ADMINISTRATION

Animals were exposed for 6 hours per day once every day at

approximately the same time each day, on GD 6 to 28 inclusive,

where GD 0 was the day of mating.

2.12.3. PRE-STUDY TRIALS

The test substance was administered as a vapor in the breathing air

of the animals. The test atmosphere was generated by an

appropriate procedure determined during pre-study trials. The

trials were performed to evaluate the optimal set of conditions and

equipment to generate a stable at mosphere at the target exposure

levels and maintain uniform conditions throughout the exposure

chambers.

2.12.4. EXPOSURE PROCEDURE

Group 1

Houseline nitrogen was delivered from a regulator with a

backpressure gauge via 1⁄4” tubing to a flowmeter regulated by a

metering valve. This nitrogen flow (~ 30 Lpm) was then directed

into the chamber turret of the 1.5 m 3 

# glass and stainless steel

exposure chamber where it was mixed with room air as it was

drawn into the chamber.

Groups 2, 3 and 4

Houseline nitrogen was delivered from a regulator with a

backpressure gauge through a stainle ss steel fitting to create three

flow systems: the test substanc e pressurization flow, the purge

flow and the volatilization flow.

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The nitrogen for the test subs tance pressurization flow was

delivered from a regulator with a backpressure gauge via 1⁄4”

tubing through a toggle va lve attached to a backpressure gauge into

the vapor inlet valve of the test substance cylinder. The houseline

nitrogen regulator valve was used to adjust and maintain the

pressure within the cylinder. From the pressurized cylinder, the

test substance flowed from the liquid outlet valve through another

toggle valve then through a fi lter to prevent equipment

contamination. From the filter, the test substance was directed into

a stainless steel “T” piece where on e side of the “T” was directed

towards the Group 2 exposure system, while the other side of the

“T” piece was directed towards the Groups 3 and 4 systems where

a second stainless steel “T” split the test substance flow and

directed it to the Group 3 and Group 4 exposure systems.

The exposure system set-up and deli very was the same for all test

groups. From the “T” pieces after the filter, the test substance

flowed to a liquid flowmeter with a built-in regulator via 1⁄8 ”

tubing. The outlet of the flowmeter was regulated by a metering

valve. From this metering valve, the test substance flowed via 1⁄8 ”

tubing onto the glass helix of a counter current volatilization

chamber. This glass helix was heated by a nichrome wire which

was controlled by an autotransf ormer and was inserted in the

center of the glass tube that suppor ted the helix. Due to the flow

rate necessary to reach the requi red chamber concentrations, single

flowmeter/glass helix and counter current systems were used for

Groups 2 and 3, while two identical flowmeter/glass helix and

counter current systems were used for Group 4.

The nitrogen for the purge flow syst em was directed, via 1⁄4” tubing

to single flowmeters regulated by metering valves for Groups 2

and 3 or to two flowmeters regulated by metering valves for

Group 4. The purge nitrogen was delivered via 1⁄8 ” tubing to the

bottom of the tube containing the nichrome wire. This nitrogen

flow continuously purged the area surrounding the nichrome wire

within the tube, thereby, prot ecting the wire from oxidation.

The nitrogen for the volatilizatio n system was directed via 1⁄4”

tubing to single flowmeters re gulated by metering valves for

Groups 2 and 3 or to two flowmeters regulated by metering valves

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for Group 4. From the flowmete r, the volatilization nitrogen

(Groups 2 and 3 ~ 30 Lpm; Group 4 ~ 15 Lpm) flowed via 1⁄4”

tubing to a ball and socket joint at the bottom of the volatilization

chamber. This nitrogen flow ed up through the volatilization

chamber passing over the coil and vo latilizing the test substance.

This test substance laden nitr ogen exited the top of the

volatilization chamber via 1⁄4” tubi ng, to the turret of the 1.5 m 3



# glass and stainless steel exposure chamber. As the test substance

laden nitrogen was drawn into each of the chambers, it was mixed

with room air.

See Figure 1 and Appendix M for equipment details.

2.12.5. CHAMBER OPERATION

The whole-body exposure chambers each had a volume of

approximately 1500 Liters (1.5 m 3 

# ). Each chamber was operated

at a minimum flow rate of 300 Lite rs per minute. The final airflow

was set to provide at least one ai r change (calculated by dividing

the chamber volume by the airflow rate) in 5.0 minutes (12 air

changes/hour) and a T 99 

# equilibrium time (calculated by

multiplying the air change by the expone ntial factor 4.6) of at most

23 minutes:

Grou p Airflow Rate Air Chan g e T 99



# ( L p m ) ( min ) ( min )

1 312 4.8 22

2 308 4.9 22

3 302 5.0 23

4 305 4.9 23

This chamber size and airflow rate s were considered adequate to

maintain the oxygen level at least 19% and the animal loading

factor below 5%. At the end of each exposure, all animals

generally remained in the chambe r for a minimum of 23 minutes.

During this time, each chamber was operated at approximately the

same flow rate using clean air onl y. The chambers were exhausted

through the in house filtering system , which consisted of a coarse

filter, a HEPA filter and activated charcoal.

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See Figure 1 and Appendix M for equipment details.

2.12.6. EXPOSURE CONCENTR ATION DETERMINATION

The nominal concentration was determined by weighing the

generation apparatus containing the test substance before and after

the exposure and calculating as follows:

Conc (ppm) = amount consumed (g) x 1000 (mg/g) x 1000 ( μ g/mg) x 22.4 ( μ L/ μ mole) x 292 K

exposure duration (min) x airflow (Lpm) x MW ( μ g/ μ mole) x 273 K

MW = 130.5 μ g/ μ mole



# The nominal concentration was calculated for all three test

substance-treated groups combined.

The flow of air through the chamber was monitored using

appropriate calibrated equipment. The test substance consumed

(weight difference of the cylinde r) during the exposure (kg) was

divided by the total volume of air (m 3 

# ) passing through the

chamber to calculate the nominal concentration (ppm).

During each exposure, measurements of airborne concentrations

were performed in the animals’ brea thing zone at l east 4 times using

an appropriate sampling pr ocedure and infrared (IR)

spectrophotometric analytical procedure.

See Appendix M for equipment details.

2.12.7. PARTICLE SIZE DISTRIBUTION ANALYSIS

During each week of exposure, particle size determinations were

performed using a TSI Aerodynamic Particle Sizer to characterize

the aerodynamic particle size dist ribution of any aerosol present.

The samples were drawn for 20 seconds at a flow rate of 5.0 Lpm.

The mass median aerodynamic diameter, geometric standard

deviation and total mass concentr ation were calculated based on

the amount of particles collected.

See Appendix M for equipment details.

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2.13. EXPERIMENTAL EVALUATIONS

2.13.1. VIABILITY CHECKS

Animals were observed in their cages twice daily (once in the

morning and once in the afternoon) for mortality, morbidity and

signs of severe toxicity.

2.13.2. PHYSICAL OBSERVATIONS

Exposure Observations

All visible animals were observe d as a group at least once during

each exposure.

Detailed Physical Examinations

Animals were removed from their cages and examined daily from

receipt to terminal euthanasia. Examinations included

observations of general condition, skin and fur, eyes, nose, oral

cavity, abdomen and external genita lia as well as evaluations of

respiration. Examinations were performed after exposures during

the exposures period.

2.13.3. BODY WEIGHT

Body weights were recorded for al l animals on the day of arrival

(for health monitoring; weights not included in this report) and on

presumed GD 4, 6, 9, 12, 15, 18, 20, 23, 26 and 29.

2.13.4. FOOD CONSUMPTION

Feed was available without restriction 7 days/week. Animals were

presented with a known quantity of feed each day. The weight of

the feed remaining on the following day was subtracted from the

quantity fed original to obtai n the grams consumed. Food

consumption was measured (weighed) daily from GD 4 through

GD 29.

2.14. TERMINAL OBSERVATIONS

2.14.1. NECROPSY INFORMATION

Necropsy was performed on 22 animals/group after 23 days of

exposure.

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Method of Euthanasia

Adult females: intravenous inje ction of sodium pentobarbital.

Fetuses: intraperitoneal inje ction of sodium pentobarbital.

2.14.2. TERMINAL EXAMINATIONS (SCHEDULED

TERMINATIONS)

A necropsy (macroscopic examination) comprised of an external

examination followed by an inte rnal examination (excluding the

cranial cavity) was performed. Mi nor sporadic le sions common to

this strain of animal (i.e. localized patchy hair loss) were not

sampled or recorded.

The intact uterus (ovaries at tached) was removed from the

abdominal cavity and weighed intact for visibly pregnant animals

only. Corpora lutea were counted and the number per ovary

recorded.

The uterus was then opened and the number and location of the

following were recorded for each uterine horn:

• live fetuses

• dead fetuses (recently dead, no significant degeneration)

• late embryo-fetal deaths (recognizable dead embryo/fetus

undergoing degeneration, regardless of size)

• early embryonic deaths (evide nce of implantation, but no

recognizable embryo), or stain-positive

The placenta associated with each fetus was examined

macroscopically and weighed.

For apparently non-pregnant anim als, and for apparently empty

uterine horns, the number of uterine implantation sites were

checked after staining with ammo nium sulphide [modification of

the Salewski staining technique ( Salewski, 1964 )].

The uteri and ovaries of appare ntly non-pregnant rabbits were

retained in neutral buffered 10% formalin and will be discarded at

the end of the study when aut horized by the Study Director.

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2.14.3. FETAL EXAMINATIONS

External Examinations

All live GD 29 fetuses we re individually identified within the litter

and weighed. Each live fetus wa s given a macroscopic external

examination for defects (including observation of the palate).

Dead fetuses without significant necrosis were also examined.

Late embryo-fetal deaths were examined for abnormalities to the

extent possible, observed abnorma lities were recorded and the

tissues were discarded. All resorptions were discarded.

Visceral

All fetuses were evaluated for soft tissue abnormalities using a

micro-dissection procedure similar to that described by Staples

( 1974 ) for examination of the torso. A single partial cross-section

was made through the coronal suture to permit in situ examination

of the brain in that region. During the examination, the sex of each

fetus was determined by inspection of the gonads.

Skeletal

After evaluation for soft tissue abnormalities each fetus was

processed for staining with Alizarin Red S. Fetal skeletal

specimens were evaluated for skel etal abnormalities, including any

affecting discernable cartilaginous structures, and ossification

state.

Recently-Dead Fetuses, Late Embryo-fetal Deaths

Recently-dead fetuses were processed and examined as for the live

fetuses.

Where possible, late embryo-fetal deaths were examined for

external abnormalities. Any with obvious external abnormalities

were preserved in neutral buffere d formalin for possible future

examination. All other late resorptions (and early resorptions)

were discarded.

2.15. STATISTICAL ANALYSIS

All statistical analyses were performe d using the individual animal (or litter)

as the basic experimental unit. The fo llowing data types were analyzed as

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necessary:

Continuous Data

• Maternal body weight, absolute weights and changes

• Gravid uterine adjusted body weights and changes

• Food consumption

• Fetal and placental weights per litter

Discrete Data (Count, Bi nomial, or Incidence)

• Litter size

• Corpora lutea, implantation sites, percent pre- and post-implantation

loss (resorptions and early/fetal deaths), sex ratio per litter

• Incidence of fetal external, viscer al and skeletal abnormalities per litter

2.15.1. METHOD OF ANALYSIS

Mean values of all exposure groups were compared to the mean

value for the control group at each time interval.

Continuous Data

Test article-treated groups were compared to the control using the

following procedures: If Bartlett's test for variance homogeneity

( Bartlett, 1937 ) was not significant at the 1% level, then parametric

methods were applied. If the F 1 

# approximate test for monotonicity

(described below) of dose-respons e was not significant at the 1%

level, Williams' test ( Williams’, 1971, 1972 ) for a monotonic trend

was applied. If the F 1 

# test was significant, suggesting that the

dose-response was not monotone, Dunnett's test ( Dunnett, 1955,

1964 ) was performed instead. If Ba rtlett's test was significant at

the 1% level, then logarithmic and square-root transformations

were tried. If Bartlett's testwas still significant, then nonparametric methods were applied to mean ranks. If the H 1 

# approximate test for monotonicity (described below) was not

significant at the 1% level, Shir ley's test for a monotonic trend

( Shirley, 1977 ) was applied. If the H 1 

# test was significant, then

Steel's test ( Steel, 1959 ) was performed instead.

For all parameters, significant differences between control and test

article-treated groups are expressed at the 5% ( p <0.05),

1% ( p <0.01) or the 0.1% ( p <0.001) level.

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The F 1 

# test is designed to detect significant departure from

monotonicity of an ordered set of means. The test statistic

compares the mean square, NMS, fo r the deviations of the absolute

means from the maximum likelihood means, calculated under a

constraint of monotonicity, with the usual error mean square, EMS.

The null hypothesis is that the true means are monotonically

ordered. The test statistic is F 1 

# = NMS/EMS which can be

compared with standard tables of the F -distribution with 1 and

error degrees of freedom.

The H 1 

# test is the non-parametric equivalent of the F 1 

# test. The test

statistic compares the non-monoton icity sums of squares, NRSS,

for the deviations of the mean ranks from the maximum likelihood

mean ranks, with the non-parametric equivalent of the error sums

of squares, ERSS=N(N+1)/12. The test statistic is H 1 

# =

NRSS/ERSS which can be compared to standard tables of the

χ

2 

# -distribution with 1 degree of freedom.

Discrete Data

For litter size, corpora lutea, implantation sites, percent pre- and

post-implantation loss (resorptions and early/fetal deaths) and sex

ratio per litter, test article-treated groups were compared to the

control using the following procedures: If the Jonckheere-Terpstra

test ( Jonckheere, 1954 ) was significa nt at the 5% level, then the

direction of the trend was esta blished and one-tailed step-down

testing in this direction was perfor med. If the Jonckheere-Terpstra

test was not significant at the 5% level, then the Kruskal-Wallis

test ( Kruskal and Wallis, 1952, 1953 ) was applied. If the KruskalWallis test was significant at the 5% level, the test-article treated

groups were compared to the c ontrol using exact Wilcoxon rank

sum tests ( Wilcoxon, 1945 ); otherwise, no further comparisons

were made.

For incidence of fetal external, visceral and skeletal abnormalities

per litter, test article- treated groups were compared to the control

using the following procedures: If the Cochran-Armitage test

( Armitage, 1955 ) was significant at th e 5% level, then the direction

of the trend was established and one-tailed step-down testing in

this direction was performed. If the Cochran-Armitage test was

not significant at the 5% level, then the χ 2 test ( Armitage et al

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2002 ) was applied. If the χ 2 test was significant at the 5% level,

the test-article treated groups were compared to the control using

Fisher’s Exact tests ( Fisher, 1973 ), otherwise, no further

comparisons were made.

For all parameters, significant differences between control and test

substance-treated groups are ex pressed at the 5% (p<0.05),

1% (p<0.01) or the 0.1% (p<0.001) level.

2.16. MAJOR COMPUTER SYSTEMS

The following major computer/softw are systems were utilized at

Huntingdon Life Sciences, East Millstone, New Jersey.

System Version Use

Liberate Reporting

System 2.2 Generation of report tables for the clinical

observations, body weight, food

consumption, organ weight and fetal

pathology data in MS Word formats

Microsoft Word

and/or Excel Additional formatting of report tables.

Quasar 1.0 or

higher Statistical analysis of data

REES Scientific

Environmental

Monitoring System WIN 1.5 Monitor and record temperature, humidity

and/or light intensity re adings of applicable

rooms as well as the temperature readings

of applicable refrigerators and/freezers

TSI Aerodynamic

Particle Sizer

Spectrometer 2.9 Acquisition of particle size data

Xybion Path/Tox

System 4.2.2 Direct online capture of clinical

observations, body weights, food

consumption, organ weights, anatomic and

fetal pathology data

2.17. DATA STORAGE

All raw data, preserved specimens, and retained samples, as well as the

original study protocol and the original final report will be maintained in

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the Archives of the Testing Facil ity upon completion of the study. The

Sponsor will determine the final disposition of these materials.

2.18. REGULATORY REFERENCES

2.18.1. TEST GUIDELINES

The design of this study was based on the OECD 414 and US

EPA 870.3700 guidelines for embr yo-fetal toxicity studies.

2.18.2. GOOD LABORATORY PRACTICES

This study was conducted in complia nce with the United States

Environmental Protection Agency's Good Laboratory Practices as

set forth in 40 CFR Part 792 (T SCA) and the Organization for

Economic Cooperation and Development (OECD) Principles of

Good Laboratory Practices ENV/MC/CHEM/(98)17.

2.18.3. ANIMAL WELFARE ACT COMPLIANCE

This study complied with all appropriate parts of the Animal

Welfare Act Regulations: 9 CF R Parts 1 and 2 Final Rules,

Federal Register, Volume 54 , No. 168, August 31, 1989, pp.

36112-36163 effective October 30, 1989 and 9 CFR Part 3 Animal

Welfare Standards; Final Rule, Federal Register, Volume 56, No.

32, February 15, 1991, pp. 6426- 6505 effective March 18, 1991.

2.19. PROTOCOL DEVIATION

The following protocol deviation occu rred during the study but was not

considered to have compromised the validity or integrity of the study:

The temperature, humidity, liquid flowmeter setting, airflow rate and static

pressure readings were not recorded for the Group 4 chamber at the

2.5 hour interval on Exposure Day 13. Values and settings recorded at the

2.0 and 3.0 hour intervals on this day were within the protocol required

ranges and were within the required settings.

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3. RESULTS AND DISCUSSION

3.1. CHAMBER MONITORING

( Appendix A )

Pre-study chamber distribution analyses showed the test substance was

evenly distributed within each chamber. The target and mean analytical

(IR) and nominal concentrations are summarized as follows:

Group Target

Concentration

(pp m ) Analytical

Concentration

(ppm) Nominal

Concentration

(pp m )

1 0 0

. 0 0

2 2500 2513 NA

3 10000 9945 NA

4 15000 14942 30154*

*the combined nominal concentration for all 3 exposed groups

The achieved mean exposure concentra tion for each group was very close to

the respective target concentration. The differences between measured

(total of 27400 ppm) and nominal (30154 ppm) concentrations were

considered representative of minor di fferences in calibration of the IR

spectrophotometer and/or the chamber airflows. Chamber environmental

conditions averaged 22°C and 56% relative humidity.

Mean particle size distribution measurements for the exposures are

summarized as follows:

Group Test

Substance Mass Median

Aerodynamic

Diameter

( μ m) Geometric

Standard

Deviation Total

Mass

Concentration

(mg/m 3 

# )

1 Air Control 6.244 2.117 3.62 x 10 -3



# 2 2.803 1.694 1.59 x 10 -3



# 3 2.697 1.847 1.47 x 10 -3



# 4 1.916 1.618 1.14 x 10 -3



# These results indicated that the atmos pheres were essentially vapor only, as

expected, since there was no substantial difference between the test

substance chambers and the air control chamber.

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3.2. MATERNAL DATA

3.2.1. MORTALITY

( Table 1 ; Appendix B )

There was no effect of exposur e on survival for females at

exposures up to 15,000 ppm. All females survived until the

termination of the study.

3.2.2. CLINICAL OBSERVATIONS

( Tables 2

, 3 and 4 ; Appendices C and D )

In-chamber observations were within normal limits on all exposure

days for all test groups.

There were no exposure-related findings in the test substance

exposed animals during the non-exposure intervals. Only

transient, minor, non-adverse findings were observed in these

exposure groups.

3.2.3. GESTATION BODY WEIGHTS

( Figure 2 ; Tables 5 and 6 ; Appendices E and F )

There were no exposure-related differences in body weights and

body weight gains in the test substance exposed animals during the

gestation period as compared with the air control group.

3.2.4. GESTATION FOOD CONSUMPTION

( Figure 3 ; Table 7 ; Appendix G )

There were no exposure-related differences in feed consumption in

the test substance exposed animals, compared to the air control

animals during the gestation period.

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3.2.5. MATERNAL MACROSCOPIC POSTMORTEM

EVALUATIONS

( Table 8 ; Appendix H )

There were no test substance-related macroscopic abnormalities.

Macroscopic findings were typical of the background findings seen

in rabbits of this age.

3.2.6. PREGNANCY OUTCOME AND CESAREAN SECTION

DATA

( Tables 9 and 10 ; Appendix I )

There were no test substance-related effects on pregnancy status

and pregnancy rate.

There was no difference between the groups pertaining to the

number of corpora lutea, numbe r of implantations, sex ratio

(% male) or post-implantation losses (%). The increase in preimplantation loss (%) at ≥ 10,000 ppm was unlikely affected by

treatment as these parameters were established prior to initiation of

exposure.

3.3. FETAL DATA

3.3.1. GRAVID UTERINE, PLACENTAL AND FETAL WEIGHTS

( Tables 11 and 12 ; Appendices J and K )

There were no test substance-related effects on gravid uterine,

placental or fetal weights.

Body weight changes (GD 6 to 29) were reduced at ≥ 2,500 ppm,

while adjusted body weight changes were increased when

compared with control values. This is considered due to the

exclusions of the non-pregnant animals from group means at the

end of the study.

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3.3.2. FETAL OBSERVATIONS

( Table13 ; Appendix L )

There was no evidence of an exposure-related qualitative or

quantitative increase in the incidences of external, visceral or

skeletal malformations/variations or ossifications.

4. CONCLUSION

There were no effects of exposures on su rvival, clinical signs, body weights, food

consumption or maternal or fetal findi ngs at sacrifice. Therefore, the noobserved-effect level (NOEL) for adult pr egnant female rabbits for systemic and

reproductive and developmental toxicity was 15,000 ppm of

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REFERENCES

Armitage, P. 1955. Tests for linear trends in proportions and frequencies. Biometrics

(Sept.): 375-386.

Armitage, P., Berry, G. and Matthews, J.N.S. 2002. Statistical Methods in Medical

Research . 4th edition, p.227. UK: Blackwell Science.

Bartlett, M.S. 1937. Properties of sufficiency and statistical tests. Proceedings of the

Royal Society, Series A , 160: 268-282.

Dunnett, C.W. 1955. A multiple comparison procedure for comparing several treatments

with a control. Journal of the American Statistical Association 50: 1096-1121.

Dunnett, C.W. 1964. New tables for multiple comparisons with a control. Biometrics 20-

3: 482-491.

Fisher, R.A. 1973. Statistical Methods for Research Workers . 14 th 

# edition. New York:

Hafner Publishing Company.

Jonckheere, A.R. 1954. A distribution-free k-sample test against ordered alternatives.

Biometrics 41: 133-145.

Kruskal, W.H. and Wallis, W.A. 1952. Use of Ranks in One-Criterion Variance Analysis.

Journal of the American Statistical Association , 47, 583-621.

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis 1952. Journal of the

American Statistical Association , 48, 907-911.

Salewski, E. 1964. Farbemethode zum makroskopischen machweis von

implantationsstellen am uterus der ratte. Archiv. Path. Exp. Pharmakol . 247: 367.

Shirley, E.A.C. 1977. A non-parametric equivalent of Williams’ test for contrasting

increasing dose levels of a treatment. Biometrics 33: 386-389.

Staples, R.E. 1974. Detection of visceral alteration in mammalian fetuses. Teratology 9:

A37.

Steel, R.G.D. 1959. A multiple comparison rank sum test: treatments versus control.

Biometrics 15: 560-572.

Wilcoxon, F. 1945. Individual comparisons by ranking methods. Biometrics Bulletin 1:

80-83.

Williams, D.A. 1971. A test for differences between treatment means when several dose

levels are compared with a zero dose control. Biometrics 27: 103-117.

Williams, D.A. 1972. The comparison of several dose levels with a zero dose control.

Biometrics 28: 519-531.

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CALCULATIONS

Adjusted Body Weight Day 29:

Body weight on Day 29 – Gravid Uterine Weight

Adjusted Body Weight Change Days 6 – 29:

Adjusted body weight Day 29 – Body weight Day 6

Food Consumption:

grams of food consumed per interval ÷ number of days = grams/animal/day

Preimplantation Loss:

Number of corpora lutea - Number of implantation sites

Percent Preimplantation Loss:

Number of corpora lutea - Number of implantation sites/Number of corpora lutea x 100

Postimplantation Loss:

Total number of early and late resorptions and dead fetuses

Percent Postimplantation Loss:

Number of early/late resorptions and dead fetuses/Number of implantation sites x 100

Sex Ratio (% Males):

Number of male pups/number of total pups x 100

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General Preface

General Notes

Individual animal data presented in this report may be rounded. Unrounded individual

animal data were used to calculate the reported mean and standard deviation values.

Therefore, use of the reported individual values to reproduce means, standard deviations

and/or to perform any subsequent calculations may produce minor discrepancies between

the calculated values and those presented in this report.

The data collection system used for collecti ng the in-life and post-life data divides the

study into phases. The phase utilized on this study was:

Gestation Phase (GES): Begins the on day of mating (GES 0). Animals

were exposed to the test material on GES 6 to 28.

Corresponding exposure levels for each group were as follows:

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppm

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Group 1 Chamber Generation System

and Whole-Body Exposure Chamber Figure 1

Note: Sampling Ports H-3 and H-4, used for pretest distribution sampling, were located

on center of the left wall and Sampling Port s H-9 and H-10 were located on the center of

the right wall of the chambers. Sampling Ports H-11, H-12, H-13 and H-14 were located

on the back wall of the chambers.

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Groups 2, 3 and 4 Chamber Generation System

and Whole-Body Exposure Chamber Figure 1

Notes:

1. Sampling Ports H-3 and H-4, used for pret est distribution sampling, were located

on center of the left wall and Sampling Ports H-9 and H-10 were located on the

center of the right wall of the chambers . Sampling Ports H-11, H-12, H-13 and

H-14 were located on the back wall of the chambers.

2. The generation system used for Group 4 included 2 liquid flowmeters and

volatization chambers.

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Mean Body Weights Figure 2

3300 3350 3400 3450 3500 3550 3600 3650 3700 3750 3800

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29

Gestation DayBody Weight (grams) 

# Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppm

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Mean Food Consumption Values Figure 3

100 105 110 115 120 125 130 135 140 145 150 155 160 165

4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29

Gestation DaysFood Consumption (g/day) 

# Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppmHuntingdon Life Sciences09-4348Page 41MA-252E-93-23FinalReportFemalesSummary of SurvivalTable 1Dose Group 1 2 3 4Dose Level (ppm) 0 2500 10000 15000n (%) n (%) n (%) n (%)Number of females entered on study22222222Removed before scheduled terminationDied0 (0) 0 (0) 0 (0) 0 (0)Moribund/elective sacrifice0 (0) 0 (0) 0 (0) 0 (0)Total0 (0) 0 (0) 0 (0) 0 (0)Huntingdon Life Sciences09-4348Page 42MA-252E-93-23FinalReportSummary of In-Chamber ObservationsaTable2aObservations are based on total animals present in the chamber each day.All = 100% of animals exhibiting a given observation.Exposure Day1 2 3 4 5 6 7 8 9 10 11 12 13 14Group 1 – 0 ppmWithin Normal LimitsAllAll All All All All AllAll All All All All AllAllGroup 2 – 2500 ppmWithin Normal LimitsAll All All All All All All All All All All All AllAllGroup 3 – 10000 ppmWithin Normal LimitsAll All All All All All All All All All All All AllAllGroup 4 – 15000 ppmWithin Normal LimitsAll All All All AllAll All All All All All All All AllHuntingdon Life Sciences09-4348Page 43MA-252E-93-23FinalReportSummary of In-Chamber ObservationsaTable2aObservations are based on total animals present in the chamber each day.All = 100% of animals exhibiting a given observation.Exposure Day15 16 17 18 19 20 21 22 23 24 25 26Group 1 – 0 ppmWithin Normal LimitsAllAll All All All All AllAll All All All AllGroup 2 – 2500 ppmWithin Normal LimitsAll All All All All All All All All All All AllGroup 3 – 10000 ppmWithin Normal LimitsAll All All All All All All All All All All AllGroup 4 – 15000 ppmWithin Normal LimitsAll All All All All All All All All All All All

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Summary of Daily Observations

Preface Table 3

Viability observations (cage-side) were perf ormed twice daily (once in the morning and

once in the afternoon). For summarizati on purposes, only time-points with animals

exhibiting abnormal findings were presented. Animals were considered to be within

normal limits at all other time-points.

Corresponding exposure levels for each group were as follows:

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppm

Key to Abbreviations:

AM = Morning Viability Observation

PM = Afternoon Viability ObservationHuntingdon Life Sciences09-4348Page 45MA-252E-93-23FinalReportFemalesSummary of Daily ObservationsTable 3Phase GESDay 4 6 9 10 11 12 13 14 15 15 16 16 17 17Session AM AM AM AM AM AM AM AM AM PM AM PM AM PMObservationsGroupGeneral Appearance, Red Exudate (Limbs/Tail) 4 1 1 1 1 1Dermal General, Ulceration (Limbs/Tail) 4 1 1 1 1 1Dermal General, Laceration (Limbs/Tail) 4 1Daily Observations, Decreased Fecal Volume 1 1 1 12 1 2 13 2 1 1 1 3 2 14 1 1 1 2 2 2 2Miscellaneous, Red Exudate Source Unknown 3 1Huntingdon Life Sciences09-4348Page 46MA-252E-93-23FinalReportFemalesSummary of Daily ObservationsTable 3Phase GESDay 18 18 19 19 20 20 21 21 22 22 23 23 24 24Session AM PM AM PM AM PM AM PM AM PM AM PM AM PMObservationsGroupDermal General, Ulceration (Limbs/Tail)4 1 1 1 1 1 1 1 1 1 1 1Dermal General, Scab(s)4 1 1 1Daily Observations, Decreased Fecal Volume 1 12 1 1 13 2 2 2 1 1 14 2 2 1 2Huntingdon Life Sciences09-4348Page 47MA-252E-93-23FinalReportFemalesSummary of Daily ObservationsTable 3Phase GESDay 25 25 26 26 27 27 28 29Session AM PM AM PM AM PM AM AMObservationsGroupGeneral Appearance, Ear Part Missing Unilateral 4 1 1 1 1 1Dermal General, Alopecia (Head/Neck)2 1Dermal General, Scab(s)4 1 1 1 1 1 1 1 1Daily Observations, Decreased Fecal Volume 2 1 13 1 1 2 2 14 2 2 2 2Daily Observations, No Stool3 1Miscellaneous, Red Exudate Source Unknown 4 1

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Summary of Physical Examination Findings

Preface Table 4

Physical examinations were performed once daily. During the exposure period

(Gestation Days 6 to 20), these observations were performed after daily exposures.

Number of animals examined represents the total number of animals observed for a given

interval.

For summarization purposes, descriptive co mments [i.e., location of scab(s) and/or

sore(s), etc.] are not presented in this tabl e. These data are contained in the study raw

data if needed.

Corresponding exposure levels for each group were as follows:

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppmHuntingdon Life Sciences09-4348Page 49MA-252E-93-23FinalReportFemalesSummary of Physical Examination FindingsTable 4Phase GESDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15ObservationsGroupExamined1 10 22 22 22 22 22 22 22 22 22 22 22 22 22 222 10 22 22 22 22 22 22 22 22 22 22 22 22 22 223 12 22 22 22 22 22 22 22 22 22 22 22 22 22 224 12 22 22 22 22 22 22 22 22 22 22 22 22 22 22Within Normal Limits1 10 22 22 21 22 22 22 22 22 22 22 22 22 22 222 10 21 21 20 20 21 22 22 22 22 21 21 21 21 213 12 22 21 20 19 19 20 20 21 21 20 20 20 21 214 12 20 18 18 18 18 19 18 18 18 18 18 19 19 17General Appearance, Ear Part Missing Unilateral 4 1 1 1 1 1 1 1 1 1 1 1 1 1 1General Appearance, Red Exudate (Limbs/Tail) 4 1Dermal General, Alopecia (Head/Neck)2 1 1 1 1 13 1 1 1 1 14 1 1 1 1 1 2Dermal General, Alopecia (Torso)4 1 1Dermal General, Ano-Genital Staining2 1 1Huntingdon Life Sciences09-4348Page 50MA-252E-93-23FinalReportFemalesSummary of Physical Examination FindingsTable 4Phase GESDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15ObservationsGroupDermal General, Ulceration (Limbs/Tail) 4 1Dermal General, Abrasion (Head/Neck)3 1 1 2 2 2 14 1 2 2 2 2 1 1 1 1 1 1 1Dermal General, Abrasion (Torso)2 1 1 1 1 1Dermal General, Stains on Fur (Torso)2 1 1Dermal General, Scab(s)1 13 1 1 1 1 1 1 1 1 14 1 2 2 2 2 2 3 3 3 2 2 1 1 1Huntingdon Life Sciences09-4348Page 51MA-252E-93-23FinalReportFemalesSummary of Physical Examination FindingsTable 4Phase GESDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29ObservationsGroupExamined1 22 22 22 22 22 22 22 22 22 22 22 22 22 222 22 22 22 22 22 22 22 22 22 22 22 22 22 223 22 22 22 22 22 22 22 22 22 22 22 22 22 224 22 22 22 22 22 22 22 22 22 22 22 22 22 22Within Normal Limits1 22 22 22 22 22 22 22 22 22 22 22 21 21 202 21 21 21 21 21 21 21 20 20 20 20 20 20 213 21 21 21 21 20 21 21 21 21 21 21 21 21 204 17 17 17 17 17 17 17 16 16 16 17 16 17 14General Appearance, Ear Part Missing Unilateral 4 1 1 1 1 1 1 1 1 1 1 1 1 1 1General Appearance, Red Exudate Ano-Genital Area 4 1General Appearance, Red Exudate (Limbs/Tail) 4 1 1Dermal General, Alopecia (Limbs)1 1 1 22 13 1Huntingdon Life Sciences09-4348Page 52MA-252E-93-23FinalReportFemalesSummary of Physical Examination FindingsTable 4Phase GESDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29ObservationsGroupDermal General, Alopecia (Head/Neck)2 1 1 1 1 1 1 1 1 1 1 1 1 1 13 1 1 1 1 1 1 1 1 1 1 1 1 1 14 2 2 2 2 1 1 1 2 2 2 1 2 2 2Dermal General, Alopecia (Torso)1 1 12 1 1 1 1 1 14 1 1 1 1 1 1 1 1 1 1 1 1 1 1Dermal General, Ano-Genital Staining4 1Dermal General, Ulceration (Limbs/Tail) 4 1 1 1 1 1 1 1Dermal General, Scab(s)3 14 1 1 1 1 1 1 1 2 2 2 2 2 1 1Miscellaneous, Red Exudate Source Unknown 4 1Huntingdon Life Sciences09-4348Page 53MA-252E-93-23FinalReportMean Body WeightsFemales (grams) Table5Phase GESDay 4 6 9 12 15 18 20 23 26 29Group 1 - 0 ppmMean 3327.7 3390.4 3397.8 3428.0 3499.9 3512.8 3558.9 3613.4 3658.3 3702.7SD 264.03 262.60 293.38 303.32 308.02 286.20 304.93 313.10 307.56 308.81N22 22 22 22 22 22 22 22 22 22Group 2 - 2500 ppmMean 3371.0 3431.8 3445.6 3475.6 3535.8 3555.5 3587.0 3625.5 3662.3 3693.3SD 205.98 221.89 215.41 217.60 208.41 221.49 222.69 236.83 235.96 226.93N21 21 21 21 21 21 21 21 21 21Group 3 - 10000 ppmMean 3367.5 3410.4 3399.0 3400.1 3453.6 3477.1 3500.3 3561.0 3592.7 3614.6SD 312.54 301.03 288.77 297.00 276.99 262.25 264.58 265.27 267.23 284.11N20 20 20 20 20 20 20 20 20 20Group 4 - 15000 ppmMean 3462.1 3526.5 3513.6 3531.2 3608.1 3626.1 3638.8 3681.9 3728.3 3747.6SD 225.83 232.00 232.82 232.80 246.11 240.50 244.52 252.01 248.98 288.19N22 22 22 22 22 22 22 22 22 22No statistically significant differences from control meanHuntingdon Life Sciences09-4348Page 54MA-252E-93-23FinalReportMean Body Weight ChangesFemales (grams) Table6Phase GESDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 1 - 0 ppmMean 62.7 7.3 30.2 71.9 12.9 46.1 54.6 44.9 44.4 168.5 143.9 312.3SD 52.74 56.58 43.28 49.36 90.15 71.27 38.83 42.08 38.98 105.24 68.99 127.22N22 22 22 22 22 22 22 22 22 22 22 22Group 2 - 2500 ppmMean 60.8 13.8 30.0 60.2 19.7 31.6 38.5 36.7 31.0 155.3 106.3 261.6SD 63.58 46.68 44.07 46.89 57.48 30.81 73.33 37.80 44.66 118.70 82.55 171.97N21 21 21 21 21 21 21 21 21 21 21 21Group 3 - 10000 ppmMean 42.9 -11.4 1.1 53.5 23.5 23.3 60.6 31.7 21.9 90.0 114.2 204.2SD 46.00 46.11 46.11 87.25 52.03 32.71 31.89 52.92 54.37 167.86 108.26 213.33N20 20 20 20 20 20 20 20 20 20 20 20Group 4 - 15000 ppmMean 64.4 -12.9 17.6 76.9 18.1 12.7 43.1 46.5 19.3 112.3 108.8 221.1SD 54.05 53.91 33.75 65.05 34.64 39.92 36.71 59.91 146.79 123.37 171.82 229.46N22 22 22 22 22 22 22 22 22 22 22 22No statistically significant differences from control meanHuntingdon Life Sciences09-4348Page 55MA-252E-93-23FinalReportMean Food Consumption ValuesFemales (grams/day) Table7Phase GESDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 1 - 0 ppmMean 150.3 149.0 149.6 134.4 143.9 161.2 149.6 131.5 128.1 146.6 136.4 142.6SD 42.79 38.03 36.69 40.66 44.69 36.59 36.79 36.40 30.1435.33 32.56 32.96N22 22 22 22 22 22 22 22 22 22 22 22Group 2 - 2500 ppmMean 152.0 149.5 144.5 128.0 142.9 157.7 144.2 119.4 120.2 143.6 128.1 137.7SD 36.10 33.31 36.41 36.32 46.61 36.43 35.02 40.06 41.4533.61 33.62 30.44N21 21 21 21 21 21 21 21 21 21 21 21Group 3 - 10000 ppmMean 142.4 131.5 124.3 106.2 120.5 132.4 129.6 114.5 115.9 122.5 120.0 121.5SD 33.80 33.32 36.76 49.59 54.23 47.67 46.01 43.28 44.0439.80 41.64 37.58N20 20 20 20 20 20 20 20 20 20 20 20Group 4 - 15000 ppmMean 155.7 138.8 139.7 126.4 140.2 151.3 142.7 125.2 127.5 138.4 131.8 135.8SD 33.15 36.22 38.78 48.22 47.47 47.45 41.55 48.65 47.1641.56 41.31 38.53N22 22 22 22 22 22 22 22 22 22 22 22No statistically significant differences from control mean

# Huntingdon Life Sciences 09-4348 Page 56

MA-252E-93-23 Final Report

Incidence Summary Report

for Gross Necropsy Observations

Preface Table 8

Key to Abbreviations

Oviducts/Fallop = Oviducts/Fallopian

w/ = with

Corresponding exposure levels for each group were as follows:

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppmHuntingdon Life Sciences Incidence Summary Report for Gross Necropsy Observations by Animal Printed: 26-May-10Mettlers Road Study number: 09-4348 Page: 57P.O. Box 2360 All SacrificesRabbit/New Zealand White Study start date: 08-Mar-10 Segment II/Inhalation___________________________________________________________________________________________________________________________________-- Females --Group: 1 2 3 4Number in group: 22 22 22 22-----------------------------------------------------------------------------------------------------------------------------------Within Normal Limits .............. 21 20 20 19Oviducts/FallopCyst ........................... 0 2 0 0PlacentaAbnormal Contents .............. 0 0 1 0Discolored ..................... 1 0 0 2Enlarged ....................... 0 0 0 1Small .......................... 0 0 0 1SpleenSmall .......................... 0 0 0 1Uterus w/ CervixAbsent ......................... 0 0 1 0Huntingdon Life Sciences09-4348Page 58MA-252E-93-23FinalReportFemalesSummary of PregnancyTable 9Dose Group 1 2 3 4Dose Level (ppm) 0 2500 10000 15000n (%) n (%) n (%) n (%)Number of females entered on study (mated)22 22 22 22- Non pregnant0 (0) 1 (5) 2 (9) 0 (0)- Pregnant22 (100) 21 (95) 20 (91) 22 (100)Pregnant with termination before scheduled date0 0 0 0Pregnant at scheduled termination22 21 20 22- With total implantation loss0 (0) 0 (0) 0 (0) 0 (0)- With viable fetuses22 (100) 21 (100) 20 (100) 22 (100)Huntingdon Life Sciences09-4348Page 59MA-252E-93-23FinalReportSummary of Cesarean Section DataTable 10Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 1 - 0 ppmMean 10.0 9.5 0.5 0.1 0.6 0.0 4.5 4.4 8.9 51.1 5.4 5.8SD 1.93 1.95 0.86 0.29 0.96 0.00 1.84 2.17 1.81 20.63 8.50 9.59N22 22 22 22 22 22 22 22 22 22 22 22Group 2 - 2500 ppmMean 9.2 8.8 0.1 0.1 0.2 0.0 4.1 4.4 8.5 48.7 5.2 2.5SD 1.67 1.87 0.36 0.30 0.44 0.00 1.80 1.86 1.78 19.97 9.43 4.61N21 21 21 21 21 21 21 21 21 21 21 21Group 3 - 10000 ppmMean 10.2 8.6 0.3 0.2 0.5 0.0 4.1 4.1 8.1 51.7 13.8 5.0SD 2.16 2.33 0.44 0.52 0.69 0.00 2.06 2.35 2.29 23.69 20.33 7.71N20 20 20 20 20 20 20 20 20 20 20 20Group 4 - 15000 ppmMean 10.4 9.2 0.2 0.1 0.4 0.2 4.4 4.2 8.6 52.1 11.0* 6.6SD 1.32 1.84 0.54 0.36 0.59 1.09 2.04 2.25 2.04 20.35 13.44 11.79N21 21 21 21 21 21 21 21 21 21 21 21Impl. = Implantation* = p < 0.05Huntingdon Life Sciences09-4348Page 60MA-252E-93-23FinalReportMean Gravid Uterine Adjusted Body WeightsFemales (grams) Table11Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeDay 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 1 - 0 ppmMean 3390 3703 312 504.52 3198 -192SD 263 309 127 86.445 277 110N 22 22 22 22 22 22Group 2 - 2500 ppmMean 3432 3693 262 487.06 3206 -226SD 222 227 172 83.754 200 154N 21 21 21 21 21 21Group 3 - 10000 ppmMean 3410 3615 204 468.79 3146 -265SD 301 284 213 94.317 290 213N 20 20 20 20 20 20Group 4 - 15000 ppmMean 3516 3777 261 530.42 3246 -270SD 232 260 138 76.468 251 164N 21 21 21 21 21 21Adj = Adusted, Bwt = BodyweightNo statistically significant differences from control meanHuntingdon Life Sciences09-4348Page 61MA-252E-93-23FinalReportMean Litter Weights(grams) Table12Placental Total Litter Male Fetal Female Fetal Overall FetalWeight Weight Weight Weight WeightGroup 1 - 0 ppmMean 4.8 345.7 39.7 38.2 39.3SD 0.51 61.12 4.11 5.34 3.64N22 22 22 22 22Group 2 - 2500 ppmMean 4.9 330.4 39.7 39.1 39.2SD 0.54 62.33 4.16 4.92 4.13N21 21 21 21 21Group 3 - 10000 ppmMean 5.1 314.1 40.0 39.5 39.7SD 0.74 73.41 6.10 5.74 5.67N20 20 20 19 20Group 4 - 15000 ppmMean 5.1 356.8 42.2 40.2 41.0SD 0.54 53.92 4.80 4.65 4.30N21 21 21 21 21No statistically significant differences from control mean

# Huntingdon Life Sciences 09-4348 Page 62

MA-252E-93-23 Final Report

Summary of Fetal Observations

Preface Table 13

External Exam inations ....................................................................................................... 63

Visceral Examinations ....................................................................................................... 64

Skeletal Examinations ........................................................................................................ 6 7

Ossification Parameters ..................................................................................................... 71Huntingdon Life Sciences09-4348Page 63MA-252E-93-23FinalReportSummary of Fetal ObservationsExternalExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation External Examinations 195 179 162 186 22 21 20 21Abdomen/trunkAbdomen, gastroschisis 1 1Tail, rudimentary1 1Umbilicus, hernia 1 1Paws/limbsForelimb(s), flexure(s)4 1Forelimb(s), lower, upper, thickened 1 1Forepaw(s), digit(s), second, misshapen 1 1Forepaw(s), flexure(s)3 1Hindlimb(s), flexure(s)5 1Left forepaw, digit(s), third, misshapen 1 1Huntingdon Life Sciences09-4348Page 64MA-252E-93-23FinalReportSummary of Fetal ObservationsVisceralExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Visceral Examinations 195 179 162 186 22 21 20 21AbdomenAbdominal cavity, fluid filled1 1Jejunum, cyst1 1Spleen, small1 1BrainLateral ventricle, dilated 1 1Lateral ventricle, hydrocephaly 1 1CervicalThymus gland, enlarged 1 1Thymus gland, partially undescended 2 2 4 2 1 3 †Thymus gland, partially undescended,left side 1 3 2 1 1 3 2 1 †Thymus gland, partially undescended, right side 2 4 2 2 †Thymus gland, small1 1EyeEye, small1 1HeartAtrium, distended 1 2 1 1Atrium, enlarged1 1Atrium, irregular1 1†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence.Huntingdon Life Sciences09-4348Page 65MA-252E-93-23FinalReportSummary of Fetal ObservationsVisceralExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Visceral Examinations 195 179 162 186 22 21 20 21HeartBilateral ventricles, enlarged1 1Heart, malrotated1 1Heart, small5 3Papillary muscle, rudimentary1 1Pericardium, distended 1 1Ventricle, av-valve, nodule1 1Ventricle, small1 1Ventricular septum, small, membranous, ventricular septal defect 1 1LiverAll lobes, enlarged1 1Gallbladder, absent1 1Gallbladder, rudimentary 3 1 4 1 3 1 4 1 †LungsAccessory lung lobe, absent 1 8 2 1 1 5 1 1 †Accessory lung lobe, rudimentary 1 1 1 1 1 1Accessory lung lobe, small 1 1 1 1 1 1Left lung lobe, cyst1 1Lungs (general), rudimentary1 1Lungs (general), small1 1 1 1av = atrio-ventricular†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence.Huntingdon Life Sciences09-4348Page 66MA-252E-93-23FinalReportSummary of Fetal ObservationsVisceralExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Visceral Examinations 195 179 162 186 22 21 20 21ThoraxAorta, distended1 1Aortic arch, interrupted 1 1Caudal vena cava, distended, malpositioned11Common carotid artery, branching variation, left side11Common carotid artery, displaced, left side43Subclavian artery, branching variation, left side5 4 2 6 3 4 2 5 †Subclavian artery, left side, additional6 2 5 8 5 2 4 5 †Thoracic cavity, fluid filled11Whole bodySkin/subcutis, edema11Lumbar spine, musculature, enlarged11†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence.Huntingdon Life Sciences09-4348Page 67MA-252E-93-23FinalReportSummary of Fetal ObservationsSkeletalExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21Caudal vert.Caudal vertebra(e), absent1 1Caudal vertebra(e), fused1 1Cervical vert.Cervical rib, complete with costal cart. 1 1Cervical rib, without costal cartilage 1 2 1 1 1 1GirdlesClavicle, bent 2 1 4 6 1 1 3 3Clavicle, enlarged1 1Clavicle, thickened1 1Pelvic girdle, unilateral caudal shift 3 2 1 3 2 1Pelvic girdle, unilateral cranial shift 2 2 2 2Scapula, enlarged1 1HeadFrontal, fissure(s)1 1Frontal, suture within, partially fused 1 1Frontal, unossified area(s) 10 7 6 1 7 6 5 1Hyoid cornua, bent inwards 11 7 5 8 5 4 4 5Hyoid cornua, bent outwards 1 2 1 1 1 1 1 1Interparietal, supraoccipital, bridge of ossification 2 2 1 1 1 1Interparietal, supraoccipital, fused 1 2 1 1Interparietal, supraoccipital, partially fused 1 1vert. = vertebra(e)Huntingdon Life Sciences09-4348Page 68MA-252E-93-23FinalReportSummary of Fetal ObservationsSkeletalExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21HeadNasal, suture within1 1Parietal, fissure(s)2 1 1 1Parietal, unossified area(s) 4 3 2 2 1 2Limb bonesHumerus, thickened1 1Metacarpal, thickened1 1Radius, thickened1 1Ulna, misshapen, thickened1 11st phalanx, thickened1 1Lumbar vert.Lumbar region, scoliosis 1 1Lumbar transverse process, enlarged 1 1Lumbar vertebral arch(es), absent 1 1Lumbar vertebral arch(es), misaligned 1 1Lumbar vertebral centrum(a), fused 1 1Lumbar vertebral centrum(a), misaligned 1 1Ribs13th, interrupted1 18th, branched1 1Distal, nodulated1 1vert. = vertebra(e)Huntingdon Life Sciences09-4348Page 69MA-252E-93-23FinalReportSummary of Fetal ObservationsSkeletalExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21SternebraeSternebral center(s), bridge of ossification 7 1 3 3 1 1 2 3 †Sternebral center(s), cranial to, supernumerary ossification 2 1 1 2 1 1Sternebral center(s), fused 1 1 1 1Sternebral center(s), misaligned 1 3 1 3 †Sternebral center(s), supernumerary ossification 1 1Sternebral center(s), supernumerary ossification, between 1 1 1 1Sternebral center(s), supernumerary ossification, partially fused, between 1 1Suture/fontanelAnterior fontanelle, enlarged 3 3 2 3 2 2Frontal/parietal suture, irregular 1 1Frontal suture, plaque of bone 4 2 9 3 2 6 †Frontal suture, sutural bone11Nasal suture, sutural bone1 1 1 1 1 1 1 1Parietal suture, enlarged11Parietal suture, irregular2 21 2Parietal suture, plaque of bone22Parietal suture, sutural bone4 44 3Posterior fontanelle, enlarged4 1 22 1 2Thoracic vert.18 thoracolumbar vertebrae, present1120 thoracolumbar vertebrae, present6 9 6 11 6 5 2 4Thoracic hemicentrum(a), fused11vert. = vertebra(e)†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence.Huntingdon Life Sciences09-4348Page 70MA-252E-93-23FinalReportSummary of Fetal ObservationsSkeletalExaminations Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21Thoracic vert.Thoracic vert. centrum(a), bipartite 1 1Thoracic vert. centrum(a), misshapen 1 1Thoracic vertebral arch(es), fused 1 1Thoracic vertebral arch(es), small 1 1Variants13th bilateral rib, complete 53 52 48 54 14 14 14 18 †13th bilateral rib, rudimentary 3 213th bilateral rib, short 12 8 13 11 7 6 7 9 †13th bilateral rib, short, non-articulated 1 1 113th left rib, complete 16 18 9 22 10 11 8 1113th left rib, rudimentary 1 1 1 1 1 113th left rib, rudimentary, non-articulated 3 2 5 3 2 4 †13th left rib, short 11 15 8 15 10 10 7 1013th left rib, short, non-articulated 1 4 2 4 1 4 2 413th right rib, complete 10 9 5 18 7 6 4 12 †13th right rib, rudimentary 3 1 3 2 3 1 3 213th right rib, rudimentary, non-articulated 1 2 1 1 2 113th right rib, short 25 17 7 31 15 11 6 1713th right rib, short, floating 1 113th right rib, short, non-articulated 2 2 1 4 2 2 1 3vert. = vertebra(e)†Litter incidence of finding analyzed statistically; no statistically significant difference from control incidence.Huntingdon Life Sciences09-4348Page 71MA-252E-93-23FinalReportSummary of Fetal ObservationsOssificationParameters Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21Caudal vert.Caudal vertebra centrum(a), bipartiteossified 1 1Caudal vertebra(e), asymmetrically ossified 1 1Cervical vert.Cervical hemicentrum(a), unossified 1 1Odontoid process, incompletely ossified 3 1 4 2 2 1 2 1Odontoid process, ossified 1 1Odontoid process, unossified 1 1GirdlesPubis, incompletely ossified 1 1 1 1 1 1Pubis, unossified1 1HeadAnterior supraorbital notch, incompletely ossified 6 4 10 2 1 2 4 2Hyoid body, incompletely ossified 1 1Hyoid body, unossified 3 1 8 3 1 1 4 2Hyoid cornua, unossified 1 1Interparietal, incompletely ossified 3 3 1 2Jugal, incompletely ossified 1 1Premaxilla, incompletely ossified 1 2 1 1 1 1Limb bonesMetacarpal, incompletely ossified 27 20 30 34 13 9 14 13vert. = vertebra(e)Huntingdon Life Sciences09-4348Page 72MA-252E-93-23FinalReportSummary of Fetal ObservationsOssificationParameters Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21Limb bonesMetacarpal, unossified 16 12 14 4 9 7 6 3Talus, incompletely ossified 2 1 1 1 1 1Talus, unossified1 1 1 1 1 11st phalanx, bilateralforelimb, unossified 1 12nd phalanx, bilateral, unossified 1 1 1 12nd phalanx, bilateral forelimb, incompletely ossified 36 44 24 45 17 14 14 162nd phalanx, bilateral forelimb, unossified 31 68 64 59 13 16 16 172nd phalanx, bilateral hind limb, incompletely ossified 3 10 13 7 3 7 8 42nd phalanx, bilateral hind limb, unossified 5 5 5 3 3 22nd phalanx, left forelimb, incompletely ossified 8 2 11 9 6 2 8 72nd phalanx, left forelimb, unossified 3 6 6 4 3 6 5 42nd phalanx, right forelimb, incompletely ossified 2 10 7 8 2 7 6 62nd phalanx, right forelimb, unossified 2 2 4 5 2 2 4 52nd phalanx, right hind limb,incompletely ossified 1 1 1 1Epiphyses, incompletely ossified 26 28 22 20 12 11 10 9Epiphyses, unossified 7 4 3 3 6 4 2 2Phalanx, bilateral forelimb, incompletely ossified1 31 2Phalanx, bilateralforelimb, unossified11Phalanx, incompletely ossified11Phalanx, unossified11SternebraeOmosternum, ossified22Huntingdon Life Sciences09-4348Page 73MA-252E-93-23FinalReportSummary of Fetal ObservationsOssificationParameters Table13Fetuses LittersGroup 1 2 3 4 1 2 3 4Observation Skeletal Examinations 195 179 162 186 22 21 20 21SternebraeSternebral center(s), bipartite ossified 1 2 1 1Thoracic vert.Thoracic vert. centrum(a),asymmetrically ossified 2 1Thoracic vert. centrum(a), bipartite ossified 1 1Thoracic vert. centrum(a), dumbbell ossified 1 1Thoracic vert. centrum(a), incompletely ossified 1 1VariantsSternebra(e), incompletely ossified 29 30 31 28 13 14 16 13Sternebra(e), unossified 22 46 47 35 9 12 13 14vert. = vertebra(e)Huntingdon Life Sciences09-4348Page 74MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median AerodynamicDiameter, TMC = Total Mass ConcentrationGroup 1 - 0 ppmDayDateExposureNumberNominalAnalytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)1 14-Mar-10 1 0 0.0 0.0 0.0 0.0 0.0 22 512 15-Mar-10 2 0 0.0 0.0 0.0 0.0 0.0 22 523 16-Mar-10 3 0 0.0 0.0 0.0 0.0 0.0 21 524 17-Mar-10 4 0 0.0 0.0 0.0 0.0 0.0 1.965 1.883 2.63E-03 22 535 18-Mar-10 5 0 0.0 0.0 0.0 0.0 0.0 21 546 19-Mar-10 6 0 0.0 0.0 0.0 0.0 0.0 21 537 20-Mar-10 7 0 0.0 0.0 0.0 0.0 0.0 21 548 21-Mar-10 8 0 0.0 0.0 0.0 0.0 0.0 21 539 22-Mar-10 9 0 0.0 0.0 0.0 0.0 0.0 21 5610 23-Mar-10 10 0 0.0 0.0 0.0 0.0 0.0 21 5811 24-Mar-10 11 0 0.0 0.0 0.0 0.0 0.0 2.418 1.845 1.11E-03 21 5212 25-Mar-10 12 0 0.0 0.0 0.0 0.0 0.0 21 5113 26-Mar-10 13 0 0.0 0.0 0.0 0.0 0.0 21 5214 27-Mar-10 14 0 0.0 0.0 0.0 0.0 0.0 22 5115 28-Mar-10 15 0 0.0 0.0 0.0 0.0 0.0 21 5116 29-Mar-10 16 0 0.0 0.0 0.0 0.0 0.0 20 5517 30-Mar-10 17 0 0.0 0.0 0.0 0.0 0.0 20 5118 31-Mar-10 18 0 0.0 0.0 0.0 0.0 0.0 3.561 1.978 2.09E-03 20 54Huntingdon Life Sciences09-4348Page 75MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median AerodynamicDiameter, TMC = Total Mass ConcentrationGroup 1 - 0 ppmDayDateExposureNumberNominalAnalytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)19 1-Apr-10 19 0 0.0 0.0 0.0 0.0 0.0 21 5420 2-Apr-10 20 0 0.0 0.0 0.0 0.0 0.0 21 5621 3-Apr-10 21 0 0.0 0.0 0.0 0.0 0.0 20 5522 4-Apr-10 22 0 0.0 0.0 0.0 0.0 0.0 21 5423 5-Apr-10 23 0 0.0 0.0 0.0 0.0 0.0 17.03 2.763 8.65E-03 22 5424 6-Apr-10 24 0 0.0 0.0 0.0 0.0 0.0 21 5625 7-Apr-10 25 0 0.0 0.0 0.0 0.0 0.0 19 6126 8-Apr-10 26 0 0.0 0.0 0.0 0.0 0.0 17 62Mean 00.06.2442.1173.62E-032154S.D.00.07.2220.4343.41E-0313Huntingdon Life Sciences09-4348Page 76MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not applicable.*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results.Group 2 - 2500 ppmDayDateExposureNumberNominal*Analytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)1 14-Mar-10 1 NA 2100 1100 2700 2200 2500 22 502 15-Mar-10 2 NA 2500 3100 2200 2800 2000 22 503 16-Mar-10 3 NA 2500 3800 2000 2000 2000 21 554 17-Mar-10 4 NA 2400 2400 2400 2800 2100 1.304 1.513 1.58E-03 22 535 18-Mar-10 5 NA 2600 2600 2900 2400 2300 23 576 19-Mar-10 6 NA 2400 2500 2500 2400 2200 21 547 20-Mar-10 7 NA 2500 2500 2500 2100 3000 22 578 21-Mar-10 8 NA 2500 2500 2600 2500 2300 21 569 22-Mar-10 9 NA 2400 1800 2700 2600 2500 21 6210 23-Mar-10 10 NA 2400 2200 2500 2300 2400 21 6211 24-Mar-10 11 NA 2500 2500 2300 2600 2400 2.014 1.796 9.13E-04 21 5412 25-Mar-10 12 NA 2600 3000 2700 2300 2400 21 5313 26-Mar-10 13 NA 2800 3200 2800 2800 2500 22 5614 27-Mar-10 14 NA 2700 2900 2900 2300 2600 23 5315 28-Mar-10 15 NA 2600 2400 2100 3100 2700 22 5116 29-Mar-10 16 NA 2500 2600 2600 2400 2400 22 5717 30-Mar-10 17 NA 2500 2900 2500 2200 2200 22 5418 31-Mar-10 18 NA 2700 2700 2700 3000 2400 6.359 1.709 2.01E-03 22 53Huntingdon Life Sciences09-4348Page 77MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not applicable.*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results.Group 2 - 2500 ppmDayDateExposureNumberNominal*Analytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)19 1-Apr-10 19 NA 2500 2400 2300 2400 2800 22 5620 2-Apr-10 20 NA 2500 2600 2400 2400 2600 22 5821 3-Apr-10 21 NA 2500 2800 2600 2400 2100 22 5822 4-Apr-10 22 NA 2800 3000 2900 2800 2600 22 5723 5-Apr-10 23 NA 2700 3000 2700 2400 2500 1.535 1.759 1.84E-03 23 5524 6-Apr-10 24 NA 2700 2500 2600 2700 2900 22 5925 7-Apr-10 25 NA 2300 2500 2000 2300 2300 19 5826 8-Apr-10 26 NA 2500 2500 2200 2600 2600 18 59Mean NA2513 2.803 1.694 1.59E-03 22 56S.D. 337 2.389 0.126 4.82E-04 1 3Huntingdon Life Sciences09-4348Page 78MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not applicable.*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results.Group 3 - 10000 ppmDayDateExposureNumberNominal*Analytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)1 14-Mar-10 1 NA 9800 10000 9300 10000 9900 22 472 15-Mar-10 2 NA 10000 10000 9800 10000 10000 23 493 16-Mar-10 3 NA 10000 9800 11000 10000 10000 21 544 17-Mar-10 4 NA 10000 11000 10000 10000 10000 1.675 1.756 2.00E-03 23 535 18-Mar-10 5 NA 10000 10000 11000 10000 10000 22 556 19-Mar-10 6 NA 9900 9700 10000 10000 10000 22 537 20-Mar-10 7 NA 10000 9900 10000 11000 10000 22 528 21-Mar-10 8 NA 9800 9700 9600 10000 10000 22 519 22-Mar-10 9 NA 9600 10000 9400 9600 9500 21 5610 23-Mar-10 10 NA 9700 9500 9300 10000 10000 21 5811 24-Mar-10 11 NA 10000 10000 10000 10000 10000 2.159 1.805 8.37E-04 22 5312 25-Mar-10 12 NA 10000 10000 10000 10000 10000 22 5113 26-Mar-10 13 NA 9900 10000 9600 9800 10000 22 5214 27-Mar-10 14 NA 9800 10000 9900 9300 9800 23 5115 28-Mar-10 15 NA 10000 10000 9900 9900 10000 21 5016 29-Mar-10 16 NA 10000 11000 10000 10000 10000 21 5817 30-Mar-10 17 NA 10000 10000 10000 9900 10000 22 5418 31-Mar-10 18 NA 9700 9600 9900 9300 10000 5.498 1.838 1.37E-03 22 55Huntingdon Life Sciences09-4348Page 79MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration, NA = not applicable.*Nominal concentration calculated for all 3 treated groups combined, see Group 4 for results.Group 3 - 10000 ppmDayDateExposureNumberNominal*Analytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)19 1-Apr-10 19 NA 10000 10000 10000 10000 10000 21 5520 2-Apr-10 20 NA 10000 10000 10000 10000 10000 22 5621 3-Apr-10 21 NA 10000 10000 9900 11000 10000 22 5822 4-Apr-10 22 NA 9900 10000 9900 10000 9800 22 5623 5-Apr-10 23 NA 9800 9700 9700 9800 9800 1.454 1.989 1.69E-03 22 5724 6-Apr-10 24 NA 10000 10000 10000 10000 10000 22 5825 7-Apr-10 25 NA 9800 10000 9400 9700 10000 18 5626 8-Apr-10 26 NA 9700 9400 9900 9400 10000 17 58Mean NA9945 2.697 1.847 1.47E-03 22 54S.D. 328 1.891 0.100 4.97E-04 1 3Huntingdon Life Sciences09-4348Page 80MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration.*Nominal concentration calculatedfor all 3 treated groups combined.Group 4 - 15000 ppmDayDateExposureNumberNominal*Analytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)1 14-Mar-10 1 31000 15000 15000 15000 15000 15000 22 492 15-Mar-10 2 30000 15000 15000 14000 15000 15000 23 523 16-Mar-10 3 30000 15000 14000 15000 15000 15000 21 554 17-Mar-10 4 30000 16000 16000 15000 16000 15000 1.176 1.530 1.36E-03 22 565 18-Mar-10 5 30000 15000 15000 16000 15000 15000 22 606 19-Mar-10 6 29000 15000 15000 15000 15000 15000 21 547 20-Mar-10 7 30000 15000 14000 14000 15000 15000 22 558 21-Mar-10 8 30000 15000 15000 15000 16000 14000 21 559 22-Mar-10 9 29000 14000 15000 14000 14000 14000 21 6010 23-Mar-10 10 30000 15000 15000 15000 15000 1500021 6611 24-Mar-10 11 31000 15000 14000 15000 15000 15000 1.889 1.576 9.64E-04 21 5612 25-Mar-10 12 31000 15000 14000 15000 15000 15000 21 5613 26-Mar-10 13 31000 15000 15000 15000 15000 15000 22 5514 27-Mar-10 14 31000 15000 15000 15000 14000 15000 22 5515 28-Mar-10 15 31000 15000 14000 15000 15000 14000 21 5616 29-Mar-10 16 31000 15000 16000 15000 15000 15000 22 6117 30-Mar-10 17 30000 15000 15000 15000 14000 15000 21 5618 31-Mar-10 18 30000 16000 16000 15000 16000 15000 3.271 1.797 9.63E-04 21 57Huntingdon Life Sciences09-4348Page 81MA-252E-93-23FinalReportChamber Monitoring ResultsCumulative Exposure RecordAppendix AGSD = Geometric Standard Deviation, MMAD = Mass Median Aerodynamic Diameter, TMC = Total Mass Concentration.*Nominal concentration calculatedfor all 3 treated groups combined.Group 4 - 15000 ppmDayDateExposureNumberNominal*Analytical Chamber ConcentrationParticle SizeDeterminationsChamber EnvironmentMeanMean IndividualMMADGSDTMCTemperatureHumidity(ppm)(ppm)(ppm)(μm)(mg/m3)(°C)(%)19 1-Apr-10 19 29000 15000 15000 15000 15000 16000 22 5820 2-Apr-10 20 30000 15000 15000 15000 15000 15000 22 6021 3-Apr-10 21 30000 15000 15000 15000 15000 15000 21 6222 4-Apr-10 22 30000 16000 16000 16000 15000 15000 22 6123 5-Apr-10 23 29000 15000 14000 15000 15000 15000 1.326 1.567 1.28E-03 22 6124 6-Apr-10 24 30000 15000 15000 15000 15000 15000 22 6325 7-Apr-10 25 30000 15000 15000 15000 15000 14000 21 5826 8-Apr-10 26 31000 15000 15000 15000 15000 1500018 58Mean 3015414942 1.916 1.618 1.14E-03 21 58S.D. 675 499 0.954 0.121 2.08E-04 1 4

# Huntingdon Life Sciences 09-4348 Page 82

MA-252E-93-23 Final Re p or t

Females Individual Animal Termination History Appendix B

Animal Date of Phase

Number Death Type of Death Phase Day a



# Group 1 - 0 ppm

1551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

1552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

1553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

1554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

1555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

1556 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

1557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

1558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

1559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

1560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

1561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

1562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

1563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

1564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

1565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

1566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

1567 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

1568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

1569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

1570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

1571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

1572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

a 

# First day of test article administration defined as Gestation Day 6

# Huntingdon Life Sciences 09-4348 Page 83

MA-252E-93-23 Final Re p or t

Females Individual Animal Termination History Appendix B

Animal Date of Phase

Number Death Type of Death Phase Day a



# Group 2 - 2500 ppm

2551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

2552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

2553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

2554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

2555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

2556 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

2557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

2558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

2559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

2560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

2561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

2562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

2563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

2564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

2565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

2566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

2567 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

2568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

2569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

2570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

2571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

2572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

a 

# First day of test article administration defined as Gestation Day 6

# Huntingdon Life Sciences 09-4348 Page 84

MA-252E-93-23 Final Re p or t

Females Individual Animal Termination History Appendix B

Animal Date of Phase

Number Death Type of Death Phase Day a



# Group 3 - 10000 ppm

3551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

3552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

3553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

3554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

3555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

3556 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

3557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

3558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

3559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

3560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

3561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

3562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

3563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

3564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

3565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

3566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

3567 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

3568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

3569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

3570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

3571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

3572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

a 

# First day of test article administration defined as Gestation Day 6

# Huntingdon Life Sciences 09-4348 Page 85

MA-252E-93-23 Final Re p or t

Females Individual Animal Termination History Appendix B

Animal Date of Phase

Number Death Type of Death Phase Day a



# Group 4 - 15000 ppm

4551 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

4552 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

4553 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

4554 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

4555 06 Apr 10 Terminal sacrifice GESTATION PHASE 29

4556 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

4557 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

4558 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

4559 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

4560 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

4561 07 Apr 10 Terminal sacrifice GESTATION PHASE 29

4562 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

4563 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

4564 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

4565 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

4566 08 Apr 10 Terminal sacrifice GESTATION PHASE 29

4567 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

4568 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

4569 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

4570 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

4571 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

4572 09 Apr 10 Terminal sacrifice GESTATION PHASE 29

a 

# First day of test article administration defined as Gestation Day 6

# Huntingdon Life Sciences 09-4348 Page 86

MA-252E-93-23 Final Report

Individual Daily Observations

Preface Appendix C

Viability observations (cage-side) were perf ormed twice daily (once in the morning and

once in the afternoon). For summarizati on purposes, only time-points with animals

exhibiting abnormal findings were presented. Animals were considered to be within

normal limits at all other time-points.

Key to Abbreviations:

AM = Morning Viability Observation

PM = Afternoon Viability ObservationHuntingdon Life Sciences09-4348Page 87MA-252E-93-23FinalReportFemales IndividualDailyObservations AppendixCPhase GESAnimalDay 4 6 9 10 11 12 13 14 15 15 16 16 17 17NumberObservations Session AM AM AM AMAMAM AM AM AM PM AM PM AM PMGroup 1 - 0 ppm1553Daily observations, decreased fecal volumeP1557Daily observations, decreased fecal volumeP1564Daily observations, decreased fecal volumePGroup 2 - 2500 ppm2554Daily observations, decreased fecal volumeP2557Daily observations, decreased fecal volumeP P2563Daily observations, decreased fecal volumePGroup 3 - 10000 ppm3551Daily observations,decreased fecal volumeP3555Daily observations, decreased fecal volumeP3560Daily observations, decreased fecal volumePP = presentHuntingdon Life Sciences09-4348Page 88MA-252E-93-23FinalReportFemales IndividualDailyObservations AppendixCPhase GESAnimalDay 4 6 9 10 11 12 13 14 15 15 16 16 17 17NumberObservations Session AM AM AM AMAMAM AM AM AM PM AM PM AM PMGroup 3 - 10000 ppm3561Daily observations, decreased fecal volumeP P P P P P3570Daily observations, decreased fecal volumeP PMiscellaneous, red exudate source unknownPGroup 4 - 15000 ppm4551General appearance, red exudate (limbs/tail), tail P P P P PDermal general, ulceration (limbs/tail), tailP P P P PDermal general, laceration (limbs/tail), tailP4554Daily observations, decreased fecal volumeP4565Daily observations, decreased fecal volumeP4567Daily observations, decreased fecal volumeP P P P P4569Daily observations, decreased fecal volumeP P P PP = presentHuntingdon Life Sciences09-4348Page 89MA-252E-93-23FinalReportFemales IndividualDailyObservations AppendixCPhase GESAnimalDay 18 18 19 19 20 20 21 21 22 22 23 23 24 24NumberObservations Session AM PM AM PMAMPM AM PM AM PM AM PM AM PMGroup 1 - 0 ppm1564Daily observations, decreased fecal volumePGroup 2 - 2500 ppm2563Daily observations, decreased fecal volumeP P2569Daily observations, decreased fecal volumePGroup 3 - 10000 ppm3555Daily observations, decreased fecal volumeP3561Daily observations, decreased fecal volumeP P3570Daily observations, decreased fecal volumeP P P P P PGroup 4 - 15000 ppm4551Dermal general, ulceration (limbs/tail),tail P P P P P P P P P P PDermal general, scab(s), tailP P P4561Daily observations, decreased fecal volumePP = presentHuntingdon Life Sciences09-4348Page 90MA-252E-93-23FinalReportFemales IndividualDailyObservations AppendixCPhase GESAnimalDay 18 18 19 19 20 20 21 21 22 22 23 23 24 24NumberObservations Session AM PM AM PMAMPM AM PM AM PM AM PM AM PMGroup 4 - 15000 ppm4567Daily observations, decreased fecal volumeP P4569Daily observations, decreased fecal volumeP P P PP = presentHuntingdon Life Sciences09-4348Page 91MA-252E-93-23FinalReportFemales IndividualDailyObservations AppendixCPhase GESAnimalDay 25 25 26 26 27 27 28 29NumberObservationsSession AM PM AM PM AM PM AM AMGroup 2 - 2500 ppm2555Dermal general, alopecia (head/neck)3Daily observations, decreased fecal volumeP2557Daily observations, decreased fecal volumePGroup 3 - 10000 ppm3554Daily observations, decreased fecal volumeP PDaily observations, no stoolP3555Daily observations, decreased fecal volumeP3566Daily observations, decreased fecal volumeP P P PGroup 4 - 15000 ppm4551Dermal general, scab(s), tailP P P P P P P P4557General appearance, ear part missing unilateralP P P P PDaily observations, decreased fecal volumeP P P P4561Daily observations, decreased fecal volumePP = present, 3 = extremeHuntingdon Life Sciences09-4348Page 92MA-252E-93-23FinalReportFemales IndividualDailyObservations AppendixCPhase GESAnimalDay 25 25 26 26 27 27 28 29NumberObservationsSession AM PM AM PM AM PM AM AMGroup 4 - 15000 ppm4566Miscellaneous, red exudate source unknownP4569Daily observations, decreased fecal volumeP P PP = presentHuntingdon Life Sciences09-4348Page 93MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 - 0 ppm1551Within normal limitsP P P P P P P P P P P P P P1552Within normal limitsP P P P P P P P P P P P P P1553Within normal limitsP P P P P P P P P P P P P P1554Within normal limitsP P P P P P P P P P P P P P1555Within normal limitsP P P P P P P P P P P P P P1556Within normal limitsP P P P P P P P P P P P P P1557Within normal limitsP P P P P P P P P P P P P P P1558Within normal limitsP P P P P P P P P P P P P P P1559Within normal limitsP P P P P P P P P P P P P P P1560Within normal limitsP P P P P P P P P P P P P P P1561Within normal limitsP P P P P P P P P P P P P P PP = presentHuntingdon Life Sciences09-4348Page 94MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 - 0 ppm1562Within normal limitsP P P P P P P P P P P P P P1563Within normal limitsP P P P P P P P P P P P PDermal general, scab(s), ear(s), rightP1564Within normal limitsP P P P P P P P P P P P P P1565Within normal limitsP P P P P P P P P P P P P P1566Within normal limitsP P P P P P P P P P P P P P1567Within normal limitsP P P P P P P P P P P P P P1568Within normal limitsP P P P P P P P P P P P P P P1569Within normal limitsP P P P P P P P P P P P P P P1570Within normal limitsP P P P P P P P P P P P P P P1571Within normal limitsP P P P P P P P P P P P P P PP = presentHuntingdon Life Sciences09-4348Page 95MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 - 0 ppm1572Within normal limitsP P P P P P P P P P P P P P PGroup 2 - 2500 ppm2551Within normal limitsP P P P P P P P P P P P P P2552Within normal limitsP P P P P P P P P P P P P P2553Within normal limitsP P P P P P P P P P P P P P2554Within normal limitsP P P P P P P P P P P P P P2555Within normal limitsP P P P P P P P PDermal general, alopecia (head/neck)3 3 3 3 32556 npWithin normal limitsP P P P P P P P P P P P P P2557Within normal limitsP P P P P P P P P P P P P P P2558Within normal limitsP P P P P P P P P P P P P P Pnp = not pregnant, P = present, 3 = extremeHuntingdon Life Sciences09-4348Page 96MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 2 - 2500 ppm2559Within normal limitsP P P P P P P P P P P P P P P2560Within normal limitsP P P P P P P P P P P P P P P2561Within normal limitsP P P P P P P P P P P P P P P2562Within normal limitsP P P P P P P P P P P P P P2563Within normal limitsP P P P P P P P P P P P P P2564Within normal limitsP P P P P P P P PDermal general, abrasion (torso), vaginal areaP P P P P2565Within normal limitsP P P P P P P P P P P P P P2566Within normal limitsP P P P P P P P P P P P P P2567Within normal limitsP P P P P P P P P P P P P P2568Within normal limitsP P P P P P P P P P P P P P PP = presentHuntingdon Life Sciences09-4348Page 97MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 2 - 2500 ppm2569Within normal limitsP P P P P P P P P P P P P P P2570Within normal limitsP P P P P P P P P P P P P P P2571Within normal limitsP P P P P P P P P P P P P P P2572Within normal limitsP P P P P P P P P P P P PDermal general, ano-genital staining, black2 2Dermal general, stains on fur (torso), ventral, black1 1Group 3 - 10000 ppm3551Within normal limitsP P P P P P P P P P P P P P3552Within normal limitsP P P P P P P P P P P P P P3553Within normal limitsP P P P P P P P P P P P P P3554Within normal limitsP P P P P P P P P P P P P PP = present, 1 = slight, 2 = moderateHuntingdon Life Sciences09-4348Page 98MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 - 10000 ppm3555Within normal limitsP P P P P P P P P P PDermal general, abrasion (head/neck), ear(s), rightP P P3556Within normal limitsP P P P P P P P P P P P P P P3557Within normal limitsP P P P P P P P PDermal general, scab(s), ear(s), rightP P P P P P3558Within normal limitsP P P P P P P P P P P P P P P3559Within normal limitsP P P P P P P P P P P PDermal general, abrasion (head/neck), ear(s), bilateralP P P3560Within normal limitsP P P P P P P P P P P P P P P3561Within normal limitsP P P P P P P P P P P P P P P3562Within normal limitsP P P P P P P P PDermal general, alopecia (head/neck)2 2 2 2 2P = present, 2 = moderateHuntingdon Life Sciences09-4348Page 99MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 - 10000 ppm3563Within normal limitsP P P P P P P P P P PDermal general, scab(s), ear(s), leftP P P3564 npWithin normal limitsP P P P P P P P P P P P P P3565Within normal limitsP P P P P P P P P P P P P P3566Within normal limitsP P P P P P P P P P P P P P3567Within normal limitsP P P P P P P P P P P PDermal general, abrasion (head/neck), ear(s), rightP P P3568 npWithin normal limitsP P P P P P P P P P P P P P P3569Within normal limitsP P P P P P P P P P P P P P P3570Within normal limitsP P P P P P P P P P P P P P P3571Within normal limitsP P P P P P P P P P P P P P Pnp = not pregnant, P = presentHuntingdon Life Sciences09-4348Page 100MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 - 10000 ppm3572Within normal limitsP P P P P P P P P P P P P P PGroup 4 - 15000 ppm4551Within normal limitsP P P P P P P P P P P P PGeneral appearance, red exudate (limbs/tail), tail PDermal general, ulceration (limbs/tail), tailP4552Within normal limitsP P P P P P P P P P P P P P4553Within normal limitsP P P P P P P P P P P P P P4554Within normal limitsP P P P P P P P P P P P P P4555Within normal limitsP P P P P P P P P P P P P P4556Within normal limitsP P P P P P P P P P P P P P PP = presentHuntingdon Life Sciences09-4348Page 101MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 - 15000 ppm4557Within normal limitsPGeneral appearance, ear part missing unilateralP P P P P P P P P P P P P PDermal general, alopecia (head/neck)2 2 2 2 2 2Dermal general, scab(s), ear(s)PDermal general, scab(s), ear(s), bilateralP P P P P P P P4558Within normal limitsP P P P P P P P P P P P P P P4559Within normal limitsP P P P P P P P P P P P P P P4560Within normal limitsP P PDermal general, abrasion(head/neck), ear(s), leftP PDermal general, abrasion (head/neck), ear(s), bilateral P P P P P P P P P P4561Within normal limitsP P P P P P P P P P P P P P P4562Within normal limitsP P P P P P P P P P P P P P4563Within normal limitsP P P P P P P P P P P P P PP = present, 2 = moderateHuntingdon Life Sciences09-4348Page 102MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 - 15000 ppm4564Within normal limitsP P P P P P P P P PDermal general, abrasion (head/neck), ear(s), left P P P P4565Within normal limitsP P P PDermal general, scab(s), ear(s), bilateralP P P P P PDermal general, scab(s), ear(s), unilateral P P P P4566Within normal limitsP P P P P P P P P P P P P P4567Within normal limitsP P P P P P P P P P P P P P P4568Within normal limitsP P P P P P PDermal general, scab(s), ear(s), bilateral P P P PDermal general, scab(s),ear(s), unilateral, rightP P P P4569Within normal limitsP P P P P P P P P P P P P P P4570Within normal limitsP P P P P P P P P P P P PDermal general, alopecia (torso)3 3P = present, 3 = extremeHuntingdon Life Sciences09-4348Page 103MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 - 15000 ppm4571Within normal limitsP P P P P P P P P P P P P P P4572Within normal limitsP P P P P P P P P P P P P PDermal general, alopecia (head/neck)2P = present, 2 = moderateHuntingdon Life Sciences09-4348Page 104MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 1 - 0 ppm1551Within normal limitsP P P P P P P P P P P P P P1552Within normal limitsP P P P P P P P P P P P P P1553Within normal limitsP P P P P P P P P P P P P P1554Within normal limitsP P P P P P P P P P P P P P1555Within normal limitsP P P P P P P P P P P P P P1556Within normal limitsP P P P P P P P P P P P P P1557Within normal limitsP P P P P P P P P P P P P P1558Within normal limitsP P P P P P P P P P P P P P1559Within normal limitsP P P P P P P P P P P P P P1560Within normal limitsP P P P P P P P P P P P P P1561Within normal limitsP P P P P P P P P P P P P PP = presentHuntingdon Life Sciences09-4348Page 105MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 1 - 0 ppm1562Within normal limitsP P P P P P P P P P P P P P1563Within normal limitsP P P P P P P P P P P P P P1564Within normal limitsP P P P P P P P P P P P P P1565Within normal limitsP P P P P P P P P P PDermal general, alopecia (limbs)3 3 2Dermal general, alopecia (torso)2 21566Within normal limitsP P P P P P P P P P P P P P1567Within normal limitsP P P P P P P P P P P P PDermal general, alopecia (limbs)31568Within normal limitsP P P P P P P P P P P P P P1569Within normal limitsP P P P P P P P P P P P P P1570Within normal limitsP P P P P P P P P P P P P PP = present, 2 = moderate, 3 = extremeHuntingdon Life Sciences09-4348Page 106MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 1 - 0 ppm1571Within normal limitsP P P P P P P P P P P P P P1572Within normal limitsP P P P P P P P P P P P P PGroup 2 - 2500 ppm2551Within normal limitsP P P P P P P P P P P P P P2552Within normal limitsP P P P P P P P P P P P P P2553Within normal limitsP P P P P P P P P P P P P P2554Within normal limitsP P P P P P P P P P P P P P2555Dermal general, alopecia (limbs)3Dermal general, alopecia (head/neck)3 3 3 2 2 2 2 2 3 3 3 3 3 32556 npWithin normal limitsP P P P P P P P P P P P P P2557Within normal limitsP P P P P P P P P P P P P Pnp = not pregnant, P = present, 2 = moderate, 3 = extremeHuntingdon Life Sciences09-4348Page 107MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 2 - 2500 ppm2558Within normal limitsP P P P P P P P P P P P P P2559Within normal limitsP P P P P P P P P P P P P P2560Within normal limitsP P P P P P P P P P P P P P2561Within normal limitsP P P P P P P P P P P P P P2562Within normal limitsP P P P P P P P P P P P P P2563Within normal limitsP P P P P P P P P P P P P P2564Within normal limitsP P P P P P P P P P P P P P2565Within normal limitsP P P P P P P P P P P P P P2566Within normal limitsP P P P P P P PDermal general, alopecia (torso)2 2 2 2 3 22567Within normal limitsP P P P P P P P P P P P P PP = present, 2 = moderate, 3 = extremeHuntingdon Life Sciences09-4348Page 108MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 2 - 2500 ppm2568Within normal limitsP P P P P P P P P P P P P P2569Within normal limitsP P P P P P P P P P P P P P2570Within normal limitsP P P P P P P P P P P P P P2571Within normal limitsP P P P P P P P P P P P P P2572Within normal limitsP P P P P P P P P P P P P PGroup 3 - 10000 ppm3551Within normal limitsP P P P P P P P P P P P P P3552Within normal limitsP P P P P P P P P P P P P P3553Within normal limitsP P P P P P P P P P P P P P3554Within normal limitsP P P P P P P P P P P P P P3555Within normal limitsP P P P P P P P P P P P P PP = presentHuntingdon Life Sciences09-4348Page 109MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 3 - 10000 ppm3556Within normal limitsP P P P P P P P P P P P P P3557Within normal limitsP P P P P P P P P P P P P P3558Within normal limitsP P P P P P P P P P P P P P3559Within normal limitsP P P P P P P P P P P P PDermal general, scab(s), ear(s), rightP3560Within normal limitsP P P P P P P P P P P P P P3561Within normal limitsP P P P P P P P P P P P P P3562Dermal general, alopecia (head/neck)2 2 2 2 2 2 2 2 2 2 2 2 2 23563Within normal limitsP P P P P P P P P P P P P P3564 npWithin normal limitsP P P P P P P P P P P P P P3565Within normal limitsP P P P P P P P P P P P P Pnp = not pregnant, P = present, 2 = moderateHuntingdon Life Sciences09-4348Page 110MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 3 - 10000 ppm3566Within normal limitsP P P P P P P P P P P P P P3567Within normal limitsP P P P P P P P P P P P P P3568 npWithin normal limitsP P P P P P P P P P P P P P3569Within normal limitsP P P P P P P P P P P P PDermal general, alopecia (limbs)33570Within normal limitsP P P P P P P P P P P P P P3571Within normal limitsP P P P P P P P P P P P P P3572Within normal limitsP P P P P P P P P P P P P PGroup 4 - 15000 ppm4551General appearance, red exudate (limbs/tail), tail P PDermal general, ulceration (limbs/tail), tailP P P P P P PDermal general, scab(s), tailP P P P P P Pnp = not pregnant, P = present, 3 = extremeHuntingdon Life Sciences09-4348Page 111MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 4 - 15000 ppm4552Within normal limitsP P P P P P P P P P P P P P4553Within normal limitsP P P P P P P P P P P P P P4554Within normal limitsP P P P P P P P P P P P P P4555Within normal limitsP P P P P P PDermal general, alopecia (head/neck)2 2 2 2 2 2Dermal general, alopecia (head/neck), snout24556Within normal limitsP P P P P P P P P P P P P P4557General appearance, ear part missing unilateralP P P P P P P P P P P P P PDermal general, alopecia (head/neck)2 2 2 24558Within normal limitsP P P P P P P P P P P P P P4559Within normal limitsP P P P P P P P P P P P P P4560Within normal limitsP P P P P P P P P P P P P PP = present, 2 = moderateHuntingdon Life Sciences09-4348Page 112MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 4 - 15000 ppm4561Within normal limitsP P P P P P P P P P P P P P4562Within normal limitsP P P P P P P P P P P P P P4563Within normal limitsP P P P P P P P P P P P PDermal general, ano-genital staining, yellow14564Within normal limitsP P P P P P P P P P P P P P4565Within normal limitsP P P P P P P P P P P P P P4566Within normal limitsP P P P P P P P P P P P PMiscellaneous, red exudate source unknownP4567Within normal limitsP P P P P P P P P P P P P P4568Within normal limitsP PDermal general, scab(s),ear(s), unilateral, rightP P P P P P P P P P P PP = present, 1 = slightHuntingdon Life Sciences09-4348Page 113MA-252E-93-23FinalReportFemalesIndividual Physical Examination FindingsAppendix DAnimal Phase GESNumberObservationsDay 16 17 18 19 20 21 22 23 24 25 26 27 28 29Group 4 - 15000 ppm4569Within normal limitsP P P P P P P P P P P P PGeneral appearance, red exudate ano-genital area P4570Dermal general, alopecia (torso)3 3 3 3 3 3 3 3 3 3 3 3 3 34571Within normal limitsP P P P P P P P P P P P P P4572Within normal limitsPDermal general, alopecia (head/neck)2 2 2 2 2 2 2 2 2 2 2 2 2P = present, 2 = moderate, 3 = extremeHuntingdon Life Sciences09-4348Page 114MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 1 - 0 ppm1551 3597.5 3702.4 3750.7 3804.3 3838.3 3871.7 3942.8 3956.2 4027.3 4050.91552 3822.6 3880.1 3882.5 3844.1 3879.2 3935.4 3963.1 4047.5 4074.3 4136.51553 3163.6 3157.6 3153.3 3184.0 3131.7 3166.2 3238.2 3235.4 3261.6 3336.81554 3113.4 3159.5 3179.3 3186.8 3293.6 3333.1 3357.5 3429.9 3502.2 3569.71555 3236.0 3362.3 3381.7 3435.2 3542.6 3564.9 3589.5 3670.1 3698.9 3800.01556 3133.9 3170.5 3171.7 3121.3 3211.6 3258.5 3307.3 3330.1 3375.1 3356.41557 3194.1 3141.4 3075.1 3088.6 3160.1 3136.4 3153.4 3175.1 3192.1 3216.51558 3384.7 3459.5 3498.9 3523.5 3645.2 3653.0 3688.7 3708.9 3781.8 3827.51559 3349.1 3338.8 3312.1 3334.6 3375.5 3385.9 3428.6 3427.8 3464.2 3484.91560 3500.7 3564.6 3544.1 3551.2 3582.5 3657.5 3643.2 3656.2 3663.4 3700.71561 3835.1 3859.1 3836.3 3962.9 3938.1 3935.2 4020.1 4066.8 4093.3 4091.01562 3018.6 3067.4 2923.7 2956.6 2988.9 3101.8 3130.5 3221.2 3216.5 3264.81563 3368.4 3413.8 3502.9 3576.4 3667.2 3688.3 3709.8 3825.1 3892.0 3914.41564 3840.7 3875.5 3979.2 3994.5 4132.9 3780.3 4092.7 4187.1 4204.3 4259.61565 3327.8 3429.9 3534.5 3604.7 3696.7 3766.1 3795.1 3897.8 3923.0 3944.01566 3135.1 3192.0 3201.7 3240.6 3350.9 3356.7 3388.7 3464.3 3548.2 3626.31567 3276.0 3317.1 3329.2 3386.2 3439.5 3485.1 3429.1 3526.6 3701.6 3692.61568 3176.4 3282.4 3287.5 3329.8 3445.9 3500.8 3587.3 3617.3 3667.4 3645.91569 3116.7 3163.1 3129.1 3172.2 3237.1 3279.7 3238.9 3334.7 3413.5 3474.91570 2948.6 3119.6 3065.9 3015.4 3096.2 3015.0 3106.9 3160.3 3244.9 3310.01571 3566.9 3690.1 3729.5 3727.7 3861.0 3904.2 3898.2 3970.4 3951.6 4078.51572 3104.2 3242.6 3281.7 3375.0 3482.5 3504.7 3585.8 3586.9 3586.4 3678.4Huntingdon Life Sciences09-4348Page 115MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 1 - 0 ppmMean 3327.7 3390.4 3397.8 3428.0 3499.9 3512.8 3558.9 3613.4 3658.3 3702.7SD 264.03 262.60 293.38 303.32 308.02 286.20 304.93 313.10 307.56 308.81N22 22 22 22 22 22 22 22 22 22Huntingdon Life Sciences09-4348Page 116MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 2 - 2500 ppm2551 3512.9 3457.3 3530.3 3570.3 3631.4 3674.7 3690.9 3740.3 3751.4 3764.82552 3158.1 3194.1 3218.7 3216.1 3346.8 3376.3 3353.7 3437.8 3453.6 3492.02553 3482.2 3570.4 3506.8 3490.5 3483.8 3509.8 3564.3 3616.3 3599.1 3585.52554 3344.0 3347.6 3274.6 3231.2 3256.0 3229.8 3233.3 3260.6 3284.1 3288.12555 3748.8 3845.1 3845.4 3884.1 3888.0 3916.0 3915.6 3908.2 3890.8 3907.72556 np 3366.6x 3352.7x 3325.0x 3363.8x 3354.1x 3376.8x 3379.1x 3392.9x 3392.1x 3403.9x2557 3773.5 3760.0 3706.2 3700.2 3763.4 3708.5 3717.9 3709.7 3745.8 3700.12558 3040.2 3006.5 2994.5 2996.5 3077.4 3043.3 3056.8 3081.5 3129.8 3168.12559 3605.1 3693.5 3718.8 3702.5 3772.5 3793.9 3850.2 3847.4 3873.9 3881.42560 3352.4 3348.7 3368.0 3404.3 3471.0 3532.9 3545.7 3607.7 3658.3 3726.22561 3171.0 3152.8 3135.3 3147.8 3283.7 3280.0 3311.8 3363.9 3403.0 3517.12562 3174.8 3207.9 3275.4 3329.8 3392.9 3464.1 3518.8 3587.1 3606.9 3687.82563 3367.2 3462.2 3490.3 3526.6 3512.7 3357.9 3477.2 3474.7 3501.9 3517.02564 3177.8 3274.4 3278.7 3350.2 3351.9 3413.9 3457.1 3534.3 3589.4 3632.42565 3623.4 3730.3 3703.9 3733.8 3738.9 3855.2 3908.4 3929.7 3966.0 3995.72566 3460.6 3590.5 3626.1 3576.6 3690.8 3673.9 3739.1 3806.6 3836.6 3892.72567 3295.0 3374.9 3425.6 3465.0 3521.2 3592.4 3627.3 3702.2 3771.6 3797.92568 3536.2 3619.9 3612.7 3673.9 3782.1 3812.3 3830.2 3933.4 4066.4 4113.02569 3212.8 3235.3 3258.7 3360.8 3427.5 3509.5 3549.3 3331.8 3437.4 3556.72570 3202.9 3324.2 3443.1 3501.3 3527.2 3557.0 3603.3 3629.8 3640.2 3647.82571 3341.6 3505.1 3524.1 3649.2 3722.4 3731.8 3738.3 3919.7 3906.4 3841.02572 3210.1 3366.3 3420.6 3477.6 3610.0 3632.1 3638.8 3713.6 3795.0 3846.6np = not pregnant, x = excluded from calculationHuntingdon Life Sciences09-4348Page 117MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 2 - 2500 ppmMean 3371.0 3431.8 3445.6 3475.6 3535.8 3555.5 3587.0 3625.5 3662.3 3693.3SD 205.98 221.89 215.41 217.60 208.41 221.49 222.69 236.83 235.96 226.93N21 21 21 21 21 21 21 21 21 21Huntingdon Life Sciences09-4348Page 118MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 3 - 10000 ppm3551 3045.6 3038.4 3011.7 3042.6 3063.5 3067.0 3090.7 3205.7 3231.3 3283.23552 3603.4 3617.7 3659.4 3655.7 3742.6 3767.9 3787.2 3828.6 3934.4 3979.03553 3592.8 3632.4 3606.3 3609.1 3732.6 3744.7 3764.0 3873.0 3917.8 3918.23554 3456.7 3454.6 3437.5 3459.6 3494.4 3537.7 3568.7 3624.8 3601.9 3499.53555 3031.4 3098.2 3119.2 3069.5 3023.3 3069.1 3045.9 3056.0 3100.7 3073.43556 3092.7 3093.9 3084.2 3141.2 3187.3 3212.6 3218.2 3285.1 3266.2 3190.63557 3182.3 3203.6 3174.8 3188.8 3276.3 3304.3 3307.5 3370.8 3445.2 3486.83558 3575.6 3620.1 3600.3 3630.6 3714.5 3735.1 3821.8 3881.6 3931.4 3982.83559 3680.9 3646.3 3616.1 3608.4 3733.5 3801.7 3799.5 3890.5 3881.1 3918.13560 3667.4 3676.5 3608.2 3547.8 3534.1 3516.4 3554.2 3615.4 3666.0 3722.83561 3400.1 3460.6 3336.4 3278.2 3168.9 3146.2 3233.4 3330.9 3358.2 3399.93562 3194.2 3318.1 3366.6 3406.7 3553.5 3581.5 3616.0 3704.0 3783.0 3834.63563 3132.5 3228.3 3218.4 3319.7 3404.3 3475.1 3504.4 3602.2 3658.8 3753.83564 np 3264.4x 3233.4x 3269.5x 3325.0x 3318.8x 3388.6x 3397.1x 3426.0x 3455.2x 3463.5x3565 3483.7 3528.9 3542.0 3574.7 3642.1 3688.5 3737.1 3740.4 3776.0 3792.13566 3810.7 3832.1 3806.9 3813.5 3742.2 3761.1 3728.6 3743.6 3635.6 3545.93567 3105.4 3178.2 3182.4 3163.9 3280.1 3327.8 3397.5 3440.8 3468.2 3534.83568 np 3051.0x 3162.2x 3171.2x 3282.5x 3250.6x 3368.5x 3371.1x 3347.4x 3326.6x 3432.4x3569 2836.2 2862.3 2857.6 2755.3 2946.9 3035.0 3046.1 3079.7 3181.2 3206.43570 4011.7 4068.2 4018.1 4013.9 3898.8 3735.1 3768.5 3815.1 3920.1 3978.33571 3422.6 3466.5 3448.2 3467.0 3514.0 3570.3 3558.6 3614.2 3587.7 3619.13572 3023.4 3182.1 3285.3 3255.9 3419.3 3464.6 3458.8 3516.8 3509.2 3572.2np = not pregnant, x = excluded from calculationHuntingdon Life Sciences09-4348Page 119MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 3 - 10000 ppmMean 3367.5 3410.4 3399.0 3400.1 3453.6 3477.1 3500.3 3561.0 3592.7 3614.6SD 312.54 301.03 288.77 297.00 276.99 262.25 264.58 265.27 267.23 284.11N20 20 20 20 20 20 20 20 20 20Huntingdon Life Sciences09-4348Page 120MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 4 - 15000 ppm4551 3753.4 3757.4 3724.6 3762.2 3819.5 3771.2 3805.6 3799.4 3840.8 3912.14552 3058.7 3180.9 3130.2 3188.1 3320.5 3312.8 3334.2 3392.4 3499.9 3616.14553 3223.1 3258.7 3233.4 3245.3 3332.4 3345.7 3402.1 3446.3 3558.0 3591.34554 3156.1 3263.1 3246.0 3283.2 3309.6 3340.9 3300.6 3408.1 3485.4 3476.14555 3782.9 3783.1 3767.6 3789.3 3857.5 3910.4 3917.3 3974.9 4036.6 4105.44556 3196.8 3215.2 3222.3 3216.1 3303.6 3329.4 3329.7 3311.7 3268.8 3232.14557 3442.7 3489.8 3441.3 3526.1 3577.6 3656.0 3678.7 3745.3 3622.0 3566.04558 3308.5 3283.3 3279.7 3295.5 3425.0 3404.1 3381.7 3418.3 3482.3 3550.34559 3588.5 3620.5 3572.3 3622.8 3706.2 3680.2 3706.2 3793.8 3879.3 4012.44560 3455.3 3491.2 3499.8 3542.8 3600.1 3667.1 3577.2 3680.5 3653.8 3769.44561 3483.9 3505.3 3451.6 3419.8 3541.4 3557.8 3547.5 3534.9 3574.6 3697.44562 3083.9 3118.6 3204.9 3228.1 3334.7 3351.8 3410.1 3408.8 3570.9 3554.54563 3311.9 3425.8 3442.0 3419.3 3497.6 3552.3 3589.3 3654.0 3656.9 3699.84564 3337.5 3462.4 3430.6 3427.9 3553.9 3586.6 3656.5 3665.0 3733.5 3853.24565 3616.3 3670.4 3615.9 3588.7 3601.7 3595.3 3618.2 3613.6 3693.7 3744.34566 3670.2 3844.9 3832.3 3861.2 4025.6 4037.9 4048.6 4119.3 4177.2 3994.34567 3521.0 3580.8 3434.2 3398.9 3349.8 3365.8 3375.7 3426.9 3517.9 3614.94568 3796.2 3955.2 3962.3 3926.1 4139.3 4107.3 4116.0 4184.1 4250.5 4316.84569 3710.7 3748.6 3688.0 3692.2 3615.5 3672.9 3621.1 3672.1 3682.4 3138.34570 3535.6 3661.8 3711.1 3754.3 3814.2 3829.6 3819.2 3846.3 3878.6 3980.44571 3623.5 3671.1 3777.9 3820.4 3938.5 3924.8 3971.0 4031.3 4074.7 4161.74572 3509.9 3594.6 3630.5 3677.3 3713.5 3775.2 3847.5 3874.2 3885.7 3860.7Huntingdon Life Sciences09-4348Page 121MA-252E-93-23FinalReportIndividual Body WeightsFemales (grams) AppendixEAnimal Phase GESNumberDay 4 6 9 12 15 18 20 23 26 29Group 4 - 15000 ppmMean 3462.1 3526.5 3513.6 3531.2 3608.1 3626.1 3638.8 3681.9 3728.3 3747.6SD 225.83 232.00 232.82 232.80 246.11 240.50 244.52 252.01 248.98 288.19N22 22 22 22 22 22 22 22 22 22Huntingdon Life Sciences09-4348Page 122MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 1 - 0 ppm1551 104.9 48.3 53.6 34.0 33.4 71.1 13.4 71.1 23.6 240.4 108.1 348.51552 57.5 2.4 -38.4 35.1 56.2 27.7 84.4 26.8 62.2 83.0 173.4 256.41553 -6.0 -4.3 30.7 -52.3 34.5 72.0 -2.8 26.2 75.2 80.6 98.6 179.21554 46.1 19.8 7.5 106.8 39.5 24.4 72.4 72.3 67.5 198.0 212.2 410.21555 126.3 19.4 53.5 107.4 22.3 24.6 80.6 28.8 101.1 227.2 210.5 437.71556 36.6 1.2 -50.4 90.3 46.9 48.8 22.8 45.0 -18.7 136.8 49.1 185.91557 -52.7 -66.3 13.5 71.5 -23.7 17.0 21.7 17.0 24.4 12.0 63.1 75.11558 74.8 39.4 24.6 121.7 7.8 35.7 20.2 72.9 45.7 229.2 138.8 368.01559 -10.3 -26.7 22.5 40.9 10.4 42.7 -0.8 36.4 20.7 89.8 56.3 146.11560 63.9 -20.5 7.1 31.3 75.0 -14.3 13.0 7.2 37.3 78.6 57.5 136.11561 24.0 -22.8 126.6 -24.8 -2.9 84.9 46.7 26.5 -2.3 161.0 70.9 231.91562 48.8 -143.7 32.9 32.3 112.9 28.7 90.7 -4.7 48.3 63.1 134.3 197.41563 45.4 89.1 73.5 90.8 21.1 21.5 115.3 66.9 22.4 296.0 204.6 500.61564 34.8 103.7 15.3 138.4 -352.6 312.4 94.4 17.2 55.3 217.2 166.9 384.11565 102.1 104.6 70.2 92.0 69.4 29.0 102.7 25.2 21.0 365.2 148.9 514.11566 56.9 9.7 38.9 110.3 5.8 32.0 75.6 83.9 78.1 196.7 237.6 434.31567 41.1 12.1 57.0 53.3 45.6 -56.0 97.5 175.0 -9.0 112.0 263.5 375.51568 106.0 5.1 42.3 116.1 54.9 86.5 30.0 50.1 -21.5 304.9 58.6 363.51569 46.4 -34.0 43.1 64.9 42.6 -40.8 95.8 78.8 61.4 75.8 236.0 311.81570 171.0 -53.7 -50.5 80.8 -81.2 91.9 53.4 84.6 65.1 -12.7 203.1 190.41571 123.2 39.4 -1.8 133.3 43.2 -6.0 72.2 -18.8 126.9 208.1 180.3 388.41572 138.4 39.1 93.3 107.5 22.2 81.1 1.1 -0.5 92.0 343.2 92.6 435.8Huntingdon Life Sciences09-4348Page 123MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 1 - 0 ppmMean 62.7 7.3 30.2 71.9 12.9 46.1 54.6 44.9 44.4 168.5 143.9 312.3SD 52.74 56.58 43.28 49.36 90.15 71.27 38.83 42.08 38.98 105.24 68.99 127.22N22 22 22 22 22 22 22 22 22 22 22 22Huntingdon Life Sciences09-4348Page 124MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 2 - 2500 ppm2551 -55.6 73.0 40.0 61.1 43.3 16.2 49.4 11.1 13.4 233.6 73.9 307.52552 36.0 24.6 -2.6 130.7 29.5 -22.6 84.1 15.8 38.4 159.6 138.3 297.92553 88.2 -63.6 -16.3 -6.7 26.0 54.5 52.0 -17.2 -13.6 -6.1 21.2 15.12554 3.6 -73.0 -43.4 24.8 -26.2 3.5 27.3 23.5 4.0 -114.3 54.8 -59.52555 96.3 0.3 38.7 3.9 28.0 -0.4 -7.4 -17.4 16.9 70.5 -7.9 62.62556 np -13.9x -27.7x 38.8x -9.7x 22.7x2.3x 13.8x -0.8x 11.8x 26.4x 24.8x 51.2x2557 -13.5 -53.8 -6.0 63.2 -54.9 9.4 -8.2 36.1 -45.7 -42.1 -17.8 -59.92558 -33.7 -12.0 2.0 80.9 -34.1 13.5 24.7 48.3 38.3 50.3 111.3 161.62559 88.4 25.3 -16.3 70.0 21.4 56.3 -2.8 26.5 7.5 156.7 31.2 187.92560 -3.7 19.3 36.3 66.7 61.9 12.8 62.0 50.6 67.9 197.0 180.5 377.52561 -18.2 -17.5 12.5 135.9 -3.7 31.8 52.1 39.1 114.1 159.0 205.3 364.32562 33.1 67.5 54.4 63.1 71.2 54.7 68.3 19.8 80.9 310.9 169.0 479.92563 95.0 28.1 36.3 -13.9 -154.8 119.3 -2.5 27.2 15.1 15.0 39.8 54.82564 96.6 4.3 71.5 1.7 62.0 43.2 77.2 55.1 43.0 182.7 175.3 358.02565 106.9 -26.4 29.9 5.1 116.3 53.2 21.3 36.3 29.7 178.1 87.3 265.42566 129.9 35.6 -49.5 114.2 -16.9 65.2 67.5 30.0 56.1 148.6 153.6 302.22567 79.9 50.7 39.4 56.2 71.2 34.9 74.9 69.4 26.3 252.4 170.6 423.02568 83.7 -7.2 61.2 108.2 30.2 17.9 103.2 133.0 46.6 210.3 282.8 493.12569 22.5 23.4 102.1 66.7 82.0 39.8 -217.5 105.6 119.3 314.0 7.4 321.42570 121.3 118.9 58.2 25.9 29.8 46.3 26.5 10.4 7.6 279.1 44.5 323.62571 163.5 19.0 125.1 73.2 9.4 6.5 181.4 -13.3 -65.4 233.2 102.7 335.92572 156.2 54.3 57.0 132.4 22.1 6.7 74.8 81.4 51.6 272.5 207.8 480.3np = not pregnant, x = excluded from calculationHuntingdon Life Sciences09-4348Page 125MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 2 - 2500 ppmMean 60.8 13.8 30.0 60.2 19.7 31.6 38.5 36.7 31.0 155.3 106.3 261.6SD 63.58 46.68 44.07 46.89 57.48 30.81 73.33 37.80 44.66 118.70 82.55 171.97N21 21 21 21 21 21 21 21 21 21 21 21Huntingdon Life Sciences09-4348Page 126MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 3 - 10000 ppm3551 -7.2 -26.7 30.9 20.9 3.5 23.7 115.0 25.6 51.9 52.3 192.5 244.83552 14.3 41.7 -3.7 86.9 25.3 19.3 41.4 105.8 44.6 169.5 191.8 361.33553 39.6 -26.1 2.8 123.5 12.1 19.3 109.0 44.8 0.4 131.6 154.2 285.83554 -2.1 -17.1 22.1 34.8 43.3 31.0 56.1 -22.9 -102.4 114.1 -69.2 44.93555 66.8 21.0 -49.7 -46.2 45.8 -23.2 10.1 44.7 -27.3 -52.3 27.5 -24.83556 1.2 -9.7 57.0 46.1 25.3 5.6 66.9 -18.9 -75.6 124.3 -27.6 96.73557 21.3 -28.8 14.0 87.5 28.0 3.2 63.3 74.4 41.6 103.9 179.3 283.23558 44.5 -19.8 30.3 83.9 20.6 86.7 59.8 49.8 51.4 201.7 161.0 362.73559 -34.6 -30.2 -7.7 125.1 68.2 -2.2 91.0 -9.4 37.0 153.2 118.6 271.83560 9.1 -68.3 -60.4 -13.7 -17.7 37.8 61.2 50.6 56.8 -122.3 168.6 46.33561 60.5 -124.2 -58.2 -109.3 -22.7 87.2 97.5 27.3 41.7 -227.2 166.5 -60.73562 123.9 48.5 40.1 146.8 28.0 34.5 88.0 79.0 51.6 297.9 218.6 516.53563 95.8 -9.9 101.3 84.6 70.8 29.3 97.8 56.6 95.0 276.1 249.4 525.53564 np -31.0x 36.1x 55.5x -6.2x 69.8x 8.5x 28.9x 29.2x 8.3x 163.7x 66.4x 230.1x3565 45.2 13.1 32.7 67.4 46.4 48.6 3.3 35.6 16.1 208.2 55.0 263.23566 21.4 -25.2 6.6 -71.3 18.9 -32.5 15.0 -108.0 -89.7 -103.5 -182.7 -286.23567 72.8 4.2 -18.5 116.2 47.7 69.7 43.3 27.4 66.6 219.3 137.3 356.63568 np 111.2x 9.0x 111.3x -31.9x 117.9x 2.6x -23.7x -20.8x 105.8x 208.9x 61.3x 270.2x3569 26.1 -4.7 -102.3 191.6 88.1 11.1 33.6 101.5 25.2 183.8 160.3 344.13570 56.5 -50.1 -4.2 -115.1 -163.7 33.4 46.6 105.0 58.2 -299.7 209.8 -89.93571 43.9 -18.3 18.8 47.0 56.3 -11.7 55.6 -26.5 31.4 92.1 60.5 152.63572 158.7 103.2 -29.4 163.4 45.3 -5.8 58.0 -7.6 63.0 276.7 113.4 390.1np = not pregnant, x = excluded from calculationHuntingdon Life Sciences09-4348Page 127MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 3 - 10000 ppmMean 42.9 -11.4 1.1 53.5 23.5 23.3 60.6 31.7 21.9 90.0 114.2 204.2SD 46.00 46.11 46.11 87.25 52.03 32.71 31.89 52.92 54.37 167.86 108.26 213.33N20 20 20 20 20 20 20 20 20 20 20 20Huntingdon Life Sciences09-4348Page 128MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 4 - 15000 ppm4551 4.0 -32.8 37.6 57.3 -48.3 34.4 -6.2 41.4 71.3 48.2 106.5 154.74552 122.2 -50.7 57.9 132.4 -7.7 21.4 58.2 107.5 116.2 153.3 281.9 435.24553 35.6 -25.3 11.9 87.1 13.3 56.4 44.2 111.7 33.3 143.4 189.2 332.64554 107.0 -17.1 37.2 26.4 31.3 -40.3 107.5 77.3 -9.3 37.5 175.5 213.04555 0.2 -15.5 21.7 68.2 52.9 6.9 57.6 61.7 68.8 134.2 188.1 322.34556 18.4 7.1 -6.2 87.5 25.8 0.3 -18.0 -42.9 -36.7 114.5 -97.6 16.94557 47.1 -48.5 84.8 51.5 78.4 22.7 66.6 -123.3 -56.0 188.9 -112.7 76.24558 -25.2 -3.6 15.8 129.5 -20.9 -22.4 36.6 64.0 68.0 98.4 168.6 267.04559 32.0 -48.2 50.5 83.4 -26.0 26.0 87.6 85.5 133.1 85.7 306.2 391.94560 35.9 8.6 43.0 57.3 67.0 -89.9 103.3 -26.7 115.6 86.0 192.2 278.24561 21.4 -53.7 -31.8 121.6 16.4 -10.3 -12.6 39.7 122.8 42.2 149.9 192.14562 34.7 86.3 23.2 106.6 17.1 58.3 -1.3 162.1 -16.4 291.5 144.4 435.94563 113.9 16.2 -22.7 78.3 54.7 37.0 64.7 2.9 42.9 163.5 110.5 274.04564 124.9 -31.8 -2.7 126.0 32.7 69.9 8.5 68.5 119.7 194.1 196.7 390.84565 54.1 -54.5 -27.2 13.0 -6.4 22.9 -4.6 80.1 50.6 -52.2 126.1 73.94566 174.7 -12.6 28.9 164.4 12.3 10.7 70.7 57.9 -182.9 203.7 -54.3 149.44567 59.8 -146.6 -35.3 -49.1 16.0 9.9 51.2 91.0 97.0 -205.1 239.2 34.14568 159.0 7.1 -36.2 213.2 -32.0 8.7 68.1 66.4 66.3 160.8 200.8 361.64569 37.9 -60.6 4.2 -76.7 57.4 -51.8 51.0 10.3 -544.1 -127.5 -482.8 -610.34570 126.2 49.3 43.2 59.9 15.4 -10.4 27.1 32.3 101.8 157.4 161.2 318.64571 47.6 106.8 42.5 118.1 -13.7 46.2 60.3 43.4 87.0 299.9 190.7 490.64572 84.7 35.9 46.8 36.2 61.7 72.3 26.7 11.5 -25.0 252.9 13.2 266.1Huntingdon Life Sciences09-4348Page 129MA-252E-93-23FinalReportIndividual Body Weight ChangesFemales (grams) AppendixFAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 4 - 15000 ppmMean 64.4 -12.9 17.6 76.9 18.1 12.7 43.1 46.5 19.3 112.3 108.8 221.1SD 54.05 53.91 33.75 65.05 34.64 39.92 36.71 59.91 146.79 123.37 171.82 229.46N22 22 22 22 22 22 22 22 22 22 22 22Huntingdon Life Sciences09-4348Page 130MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 1 - 0 ppm1551 166.2 154.3 158.3 149.4 163.8 171.4 163.3 152.9 132.3 158.6 149.5 155.01552 131.9 152.8 139.2 101.3 176.3 193.2 167.3 131.7 153.8 149.7 150.9 150.21553 102.5 101.0 111.6 61.1 49.4 100.6 78.6 57.9 70.7 83.6 69.1 77.91554 128.8 136.3 149.1 148.7 162.9 146.0 129.0 117.5 111.0 148.8 119.2 137.21555 174.0 185.2 183.3 148.0 180.3 197.3 184.6 136.9 136.5 177.5 152.7 167.81556 117.0 135.6 103.8 113.6 130.2 129.8 116.3 94.7 89.2 122.1 100.1 113.51557 82.9 75.8 90.3 69.3 107.5 89.5 114.3 113.7 111.8 86.3 113.3 96.81558 181.8 175.8 173.4 173.1 172.0 181.4 178.4 183.6 141.0 174.7 167.7 171.91559 102.8 100.5 107.4 87.4 116.2 116.4 96.5 93.4 99.9 104.8 96.6 101.61560 118.3 122.6 134.0 96.1 141.6 137.0 95.0 69.2 92.7 125.5 85.6 109.91561 201.8 192.7 190.0 169.2 151.2 185.3 184.6 166.2 139.5 177.1 163.4 171.81562 53.4 77.9 86.3 86.7 101.8 115.9 129.5 120.1 129.8 92.2 126.5 105.61563 191.3 184.2 174.7 167.3 188.4 182.7 154.5 129.3 128.6 179.3 137.5 162.91564 191.3 191.2 192.9 172.0 30.4 190.8 179.0 175.8 137.2 152.9 164.0 157.21565 195.4 192.7 186.6 174.4 187.6 189.1 185.0 145.0 155.4 185.9 161.8 176.51566 121.1 120.6 103.4 85.5 114.6 126.6 117.8 95.8 106.9 109.0 106.9 108.11567 188.6 183.4 183.8 185.5 188.9 195.9 197.7 167.3 113.8 186.9 159.6 176.21568 137.4 144.0 149.1 151.0 161.9 186.9 165.2 128.1 114.3 156.6 135.9 148.51569 151.6 124.0 129.9 110.4 111.7 128.6 110.5 107.9 124.8 120.4 114.4 118.01570 182.4 169.5 195.0 163.5 180.8 204.9 186.8 179.1 196.6 179.3 187.7 182.31571 190.9 164.0 155.2 153.0 155.4 176.6 165.4 139.1 140.5 159.7 148.3 155.31572 195.1 194.5 194.3 190.6 193.5 201.5 191.6 186.7 191.9 194.4 190.1 192.7Huntingdon Life Sciences09-4348Page 131MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 1 - 0 ppmMean 150.3 149.0 149.6 134.4 143.9 161.2 149.6 131.5 128.1 146.6 136.4 142.6SD 42.79 38.03 36.69 40.66 44.69 36.59 36.79 36.40 30.1435.33 32.56 32.96N22 22 22 22 22 22 22 22 22 22 22 22Huntingdon Life Sciences09-4348Page 132MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 2 - 2500 ppm2551 97.4 150.4 149.4 150.2 166.5 172.9 153.8 143.2 145.9 156.8 147.7 153.22552 129.2 126.6 125.9 123.7 125.4 117.4 126.0 121.6 140.3 124.3 129.3 126.22553 158.2 151.8 134.1 113.8 128.8 119.1 104.1 46.5 59.1 130.3 69.9 106.62554 109.0 78.6 47.8 39.7 66.4 78.276.2 79.1 66.5 61.0 73.9 66.02555 148.8 169.6 166.4 143.9 156.0 151.8 109.4 45.3 6.6 158.0 59.6 122.22556 np 104.7x 95.6x 110.9x 115.6x 119.5x 102.9x 113.5x 109.9x 120.9x 109.3x 114.8x 111.4x2557 119.7 111.7 103.9 58.4 47.9 115.6 73.8 44.3 109.8 85.5 76.0 81.82558 95.8 105.5 103.9 90.9 123.8 137.0 112.0 110.0 109.8 110.4 110.6 110.52559 166.1 157.9 158.2 152.8 161.3 181.2 168.2 136.6 117.6 160.9 140.8 153.12560 123.3 137.7 130.1 134.7 164.0 159.1 139.7 114.8 133.4 144.1 129.3 138.32561 84.4 79.2 78.5 90.9 114.4 130.4 146.8 120.7 138.0 96.4 135.2 111.62562 154.1 144.1 165.2 169.8 177.5 183.0 148.3 126.1 125.4 166.8 133.3 153.72563 195.4 191.3 180.1 112.3 26.3 91.3 135.2 123.8 102.0 122.3 120.3 121.52564 155.9 157.6 150.5 120.2 142.6 178.1 174.2 147.1 122.5 147.8 147.9 147.82565 186.0 159.7 152.5 118.9 159.8 177.7 166.9 136.4 146.1 152.0 149.8 151.22566 168.4 164.3 149.7 136.1 147.7 176.4 163.8 128.9 144.6 153.3 145.7 150.42567 150.2 162.3 148.8 134.2 156.0 175.4 164.5 154.6 135.8 153.9 151.6 153.02568 176.2 163.8 166.4 155.0 191.5 194.4 194.9 184.9 151.2 172.8 177.0 174.42569 185.1 154.7 161.5 150.5 175.9 177.4 121.9 93.3 185.6 163.1 135.1 152.92570 201.4 194.7 187.1 129.8 182.2 200.2 173.4 110.8 111.3 177.3 126.6 158.82571 192.1 183.5 179.1 169.8 187.8 199.1 187.3 154.7 85.2 182.8 142.4 167.02572 194.8 193.6 194.7 192.2 198.5 197.2 188.5 185.1 188.3 195.1 187.3 192.1np = not pregnant, x = excluded from calculationHuntingdon Life Sciences09-4348Page 133MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 2 - 2500 ppmMean 152.0 149.5 144.5 128.0 142.9 157.7 144.2 119.4 120.2 143.6 128.1 137.7SD 36.10 33.31 36.41 36.32 46.61 36.43 35.02 40.06 41.4533.61 33.62 30.44N21 21 21 21 21 21 21 21 21 21 21 21Huntingdon Life Sciences09-4348Page 134MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 3 - 10000 ppm3551 70.6 89.7 114.0 121.8 130.7 131.3 128.0 131.9 136.6 116.5 132.2 122.63552 152.6 148.5 138.0 116.7 133.1 141.7 148.0 139.0 146.9 135.2 144.6 138.93553 121.3 134.4 131.9 116.7 150.0 159.5 167.8 132.6 111.2 137.0 137.2 137.13554 141.3 110.7 109.1 107.9 132.1 135.9 108.4 39.7 1.5 116.9 49.9 89.43555 105.0 116.9 113.8 35.2 60.0 46.2 46.9 55.9 107.5 76.4 70.1 74.03556 161.3 158.4 161.6 122.4 136.9 152.6 135.7 113.2 126.0 145.9 125.0 137.73557 122.5 120.3 119.0 123.7 123.8 130.6 117.8 109.5 106.5 123.0 111.3 118.43558 140.7 127.6 131.4 112.6 145.5 161.5 165.4 151.7 158.0 133.9 158.4 143.53559 95.9 75.7 85.7 100.4 128.7 147.9 152.1 125.9 110.0 104.8 129.4 114.43560 120.5 100.2 80.1 33.9 44.6 83.5 115.6 108.6 112.7 67.4 112.3 85.03561 105.1 75.6 37.6 6.2 11.5 54.1 82.0 90.6 103.5 40.7 92.0 62.73562 188.9 189.4 188.3 185.9 193.1 191.3 195.7 191.2 192.3 189.5 193.1 190.93563 187.6 180.7 158.8 147.1 160.5 170.5 150.0 116.4 145.2 163.0 137.2 152.93564 np 124.2x 152.3x 168.6x 163.2x 167.3x 162.0x 162.2x 173.8x 170.1x 162.7x 168.7x 165.1x3565 163.4 153.2 164.8 157.3 171.1 174.0 178.1 147.5 121.8 163.4 149.1 157.83566 173.4 144.9 136.7 74.3 61.2 96.2 49.6 17.5 24.2 103.1 30.5 74.73567 133.4 118.2 111.5 108.6 136.1 146.4 128.7 123.2 127.6 122.6 126.5 124.13568 np 167.1x 166.4x 174.5x 170.9x 175.6x 172.0x 160.0x 164.0x 161.8x 171.9x 162.0x 168.0x3569 154.2 119.0 79.4 77.4 124.7 138.6 131.0 122.0 106.0 105.6 119.7 111.13570 142.2 131.5 106.3 39.5 10.0 24.0 38.0 60.0 83.1 65.0 60.4 63.23571 170.3 144.5 142.1 149.4 164.8 172.6 162.3 146.5 121.7 153.4 143.5 149.53572 199.1 189.7 175.8 187.0 191.0 190.3 190.4 166.3 176.4 186.5 177.7 183.1np = not pregnant, x = excluded from calculationHuntingdon Life Sciences09-4348Page 135MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 3 - 10000 ppmMean 142.4 131.5 124.3 106.2 120.5 132.4 129.6 114.5 115.9 122.5 120.0 121.5SD 33.80 33.32 36.76 49.59 54.23 47.67 46.01 43.28 44.0439.80 41.64 37.58N20 20 20 20 20 20 20 20 20 20 20 20Huntingdon Life Sciences09-4348Page 136MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 4 - 15000 ppm4551 138.9 127.9 110.3 75.3 87.7 96.6 127.4 110.2 120.4 99.8 119.4 107.44552 123.3 118.9 124.0 116.6 146.7 156.4 131.0 181.3 168.1 130.8 160.1 142.34553 110.8 102.0 125.6 134.9 137.1 162.3 155.1 142.5 151.1 130.3 149.6 137.84554 136.5 153.0 144.4 134.8 147.8 178.9 135.7 108.4 77.1 149.8 107.1 133.14555 171.7 135.9 133.3 147.4 167.1 173.1 184.9 169.4 168.6 149.8 174.3 159.44556 133.4 132.7 138.9 125.8 145.8 158.2 127.8 51.5 74.5 139.0 84.6 117.74557 147.2 154.2 162.0 133.2 159.6 175.2 143.5 45.3 42.0 155.5 76.9 124.84558 93.4 87.1 84.1 84.2 101.3 111.7 104.0 108.3 121.7 92.4 111.3 99.84559 154.6 181.9 173.5 158.8 172.9 176.2 165.0 159.8 159.1 172.4 161.3 168.14560 182.3 166.0 161.6 157.4 149.3 118.4 131.4 152.0 147.8 152.9 143.7 149.34561 107.2 70.2 60.8 65.3 70.8 90.1 73.9 46.7 129.9 70.1 83.5 75.34562 195.4 168.8 180.8 191.3 195.7 195.4 198.4 182.9 161.3 185.8 180.9 183.84563 189.9 156.5 162.0 140.6 157.0 181.6 172.6 145.8 135.4 158.0 151.3 155.34564 168.8 140.9 133.2 133.8 143.6 177.8 169.6 161.7 176.7 143.6 169.3 153.64565 146.1 117.0 109.1 61.8 89.4 116.6 97.2 88.7 121.6 97.5 102.5 99.44566 196.1 173.7 184.1 182.4 191.5 183.4 191.1 142.8 88.5 183.0 140.8 166.54567 131.5 62.0 67.7 44.4 45.4 61.6 73.0 85.7 118.3 55.8 92.3 70.14568 193.2 159.7 161.4 162.2 172.9 191.8 178.6 150.3 151.7 168.0 160.2 165.04569 128.1 108.8 100.3 21.9 39.1 34.6 55.4 44.9 12.5 62.8 37.6 52.94570 201.0 179.7 182.7 168.3 181.3 195.5 184.6 183.8 193.5 180.5 187.3 183.24571 192.4 190.4 194.6 199.0 198.5 202.8 188.7 190.5 191.4 196.6 190.2 194.34572 183.9 166.1 179.1 142.2 183.6 190.7 149.8 101.8 93.9 171.0 115.2 149.2Huntingdon Life Sciences09-4348Page 137MA-252E-93-23FinalReportIndividual Food Consumption ValuesFemales (grams/day) AppendixGAnimal Phase GESNumberDay 4-6 6-9 9-12 12-15 15-18 18-20 20-23 23-26 26-29 6-20 20-29 6-29Group 4 - 15000 ppmMean 155.7 138.8 139.7 126.4 140.2 151.3 142.7 125.2 127.5 138.4 131.8 135.8SD 33.15 36.22 38.78 48.22 47.47 47.45 41.55 48.65 47.1641.56 41.31 38.53N22 22 22 22 22 22 22 22 22 22 22 22

# Huntingdon Life Sciences 09-4348 Page 138

MA-252E-93-23 Final Report

Individual Animal Gross

and Microscopic Observations

Preface Appendix H

Key to Abbreviations

Oviducts/Fallop = Oviducts/Fallopian

w/ = with

Corresponding exposure levels for each group were as follows:

Group 1 - 0 ppm

Group 2 - 2500 ppm

Group 3 - 10000 ppm

Group 4 - 15000 ppmHuntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers Printed: 26-May-10Mettlers Road Study number: 09-4348 Page: 139P.O. Box 2360Rabbit/New Zealand White Study start date: 08-Mar-10 Segment II/Inhalation___________________________________________________________________________________________________________________________________Animal Group/ Date and time Date data Oprnumber Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measurements,Severity levels-----------------------------------------------------------------------------------------------------------------------------------1551 F 1/1 06-Apr-10 09:32 06-Apr-10 358 Within Normal Limits1552 F 1/1 06-Apr-10 09:27 06-Apr-10 322 Within Normal Limits1553 F 1/1 06-Apr-10 09:59 06-Apr-10 322 Within Normal Limits1554 F 1/1 06-Apr-10 10:06 06-Apr-10 250 Within Normal Limits1555 F 1/1 06-Apr-10 10:41 06-Apr-10 358 Within Normal Limits1556 F 1/1 06-Apr-10 11:23 06-Apr-10 362 Within Normal Limits1557 F 1/1 07-Apr-10 09:12 07-Apr-10 362 Within Normal Limits1558 F 1/1 07-Apr-10 09:13 07-Apr-10 322 Within Normal Limits1559 F 1/1 07-Apr-10 09:43 07-Apr-10 358 Within Normal Limits1560 F 1/1 07-Apr-10 09:52 07-Apr-10 322 Within Normal Limits1561 F 1/1 07-Apr-10 10:45 07-Apr-10 362 Within Normal Limits1562 F 1/1 08-Apr-10 09:15 08-Apr-10 362 Within Normal Limits1563 F 1/1 Placenta08-Apr-10 09:29 08-Apr-10 200 Discolored, , Tan, Areas, 0.2 - 0.5 cm, Moderate#11Tissues marked 'Within Normal Limits':08-Apr-10 09:33 08-Apr-10 200 Body (entire)1564 F 1/1 08-Apr-10 10:01 08-Apr-10 362 Within Normal Limits1565 F 1/1 08-Apr-10 10:16 08-Apr-10 205 Within Normal Limits1566 F 1/1 08-Apr-10 10:48 08-Apr-10 222 Within Normal Limits1567 F 1/1 08-Apr-10 11:39 08-Apr-10 222 Within Normal Limits1568 F 1/1 09-Apr-10 09:15 09-Apr-10 358 Within Normal Limits1569 F 1/1 09-Apr-10 09:23 09-Apr-10 322 Within Normal Limits1570 F 1/1 09-Apr-10 09:57 09-Apr-10 358 Within Normal LimitsHuntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers Printed: 26-May-10Mettlers Road Study number: 09-4348 Page: 140P.O. Box 2360Rabbit/New Zealand White Study start date: 08-Mar-10 Segment II/Inhalation___________________________________________________________________________________________________________________________________Animal Group/ Date and time Date data Oprnumber Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measurements,Severity levels-----------------------------------------------------------------------------------------------------------------------------------1571 F 1/1 09-Apr-10 10:37 09-Apr-10 362 Within Normal Limits1572 F 1/1 09-Apr-10 11:16 09-Apr-10 362 Within Normal Limits2551 F 2/1 06-Apr-10 09:41 06-Apr-10 362 Within Normal Limits2552 F 2/1 06-Apr-10 09:25 06-Apr-10 250 Within Normal Limits2553 F 2/1 06-Apr-10 10:03 06-Apr-10 358 Within Normal Limits2554 F 2/1 06-Apr-10 10:34 06-Apr-10 222 Within Normal Limits2555 F 2/1 06-Apr-10 10:41 06-Apr-10 46 Within Normal Limits2556 F 2/1 Oviducts/Fallop13-Apr-10 12:43 06-Apr-10 200 Cyst, Right, Clear, Fluid, 0.2 - 0.5 cm,Tissues marked 'Within Normal Limits':06-Apr-10 11:01 06-Apr-10 200 Body (entire) Placenta2557 F 2/1 07-Apr-10 09:15 07-Apr-10 358 Within Normal Limits2558 F 2/1 Oviducts/Fallop07-Apr-10 09:14 07-Apr-10 205 Cyst, Left, Clear, Fluid, 0.2 - 0.5 cm,Tissues marked 'Within Normal Limits':07-Apr-10 09:18 07-Apr-10 205 Body (entire) Placenta2559 F 2/1 07-Apr-10 09:46 07-Apr-10 200 Within Normal Limits2560 F 2/1 07-Apr-10 10:01 07-Apr-10 205 Within Normal Limits2561 F 2/1 07-Apr-10 10:52 07-Apr-10 222 Within Normal Limits2562 F 2/1 08-Apr-10 09:29 08-Apr-10 222 Within Normal Limits2563 F 2/1 08-Apr-10 09:28 08-Apr-10 205 Within Normal Limits2564 F 2/1 08-Apr-10 09:58 08-Apr-10 358 Within Normal Limits2565 F 2/1 08-Apr-10 10:09 08-Apr-10 322 Within Normal Limits2566 F 2/1 08-Apr-10 10:48 08-Apr-10 362 Within Normal Limits2567 F 2/1 08-Apr-10 11:30 08-Apr-10 362 Within Normal LimitsHuntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers Printed: 26-May-10Mettlers Road Study number: 09-4348 Page: 141P.O. Box 2360Rabbit/New Zealand White Study start date: 08-Mar-10 Segment II/Inhalation___________________________________________________________________________________________________________________________________Animal Group/ Date and time Date data Oprnumber Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measurements,Severity levels-----------------------------------------------------------------------------------------------------------------------------------2568 F 2/1 09-Apr-10 09:24 09-Apr-10 222 Within Normal Limits2569 F 2/1 09-Apr-10 09:27 09-Apr-10 250 Within Normal Limits2570 F 2/1 09-Apr-10 10:04 09-Apr-10 222 Within Normal Limits2571 F 2/1 09-Apr-10 10:45 09-Apr-10 358 Within Normal Limits2572 F 2/1 09-Apr-10 11:19 09-Apr-10 358 Within Normal Limits3551 F 3/1 Placenta06-Apr-10 09:43 06-Apr-10 222 Abnormal Contents, , Red, Gelatinous material, , ModerateNumber 2.Tissues marked 'Within Normal Limits':06-Apr-10 09:45 06-Apr-10 222 Body (entire)3552 F 3/1 06-Apr-10 09:49 06-Apr-10 205 Within Normal Limits3553 F 3/1 06-Apr-10 10:01 06-Apr-10 46 Within Normal Limits3554 F 3/1 06-Apr-10 10:34 06-Apr-10 362 Within Normal Limits3555 F 3/1 06-Apr-10 11:30 06-Apr-10 222 Within Normal Limits3556 F 3/1 07-Apr-10 09:22 07-Apr-10 222 Within Normal Limits3557 F 3/1 07-Apr-10 09:22 07-Apr-10 46 Within Normal Limits3558 F 3/1 07-Apr-10 09:47 07-Apr-10 250 Within Normal Limits3559 F 3/1 07-Apr-10 09:57 07-Apr-10 46 Within Normal Limits3560 F 3/1 07-Apr-10 10:47 07-Apr-10 358 Within Normal Limits3561 F 3/1 07-Apr-10 11:27 07-Apr-10 362 Within Normal Limits3562 F 3/1 Uterus w/ Cervix08-Apr-10 09:11 08-Apr-10 358 Absent, Right Horn, , , ,Some rudimentary uterine tissue present.Tissues marked 'Within Normal Limits':08-Apr-10 09:24 08-Apr-10 358 Body (entire) Placenta3563 F 3/1 08-Apr-10 09:34 08-Apr-10 250 Within Normal LimitsHuntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers Printed: 26-May-10Mettlers Road Study number: 09-4348 Page: 142P.O. Box 2360Rabbit/New Zealand White Study start date: 08-Mar-10 Segment II/Inhalation___________________________________________________________________________________________________________________________________Animal Group/ Date and time Date data Oprnumber Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measurements,Severity levels-----------------------------------------------------------------------------------------------------------------------------------3564 F 3/1 08-Apr-10 09:56 08-Apr-10 222 Within Normal Limits3565 F 3/1 08-Apr-10 10:17 08-Apr-10 250 Within Normal Limits3566 F 3/1 08-Apr-10 10:49 08-Apr-10 358 Within Normal Limits3567 F 3/1 09-Apr-10 09:17 09-Apr-10 205 Within Normal Limits3568 F 3/1 09-Apr-10 09:21 09-Apr-10 46 Within Normal Limits3569 F 3/1 09-Apr-10 10:05 09-Apr-10 46 Within Normal Limits3570 F 3/1 09-Apr-10 10:51 09-Apr-10 222 Within Normal Limits3571 F 3/1 09-Apr-10 11:27 09-Apr-10 222 Within Normal Limits3572 F 3/1 09-Apr-10 11:54 09-Apr-10 362 Within Normal Limits4551 F 4/1 06-Apr-10 09:31 06-Apr-10 200 Within Normal Limits4552 F 4/1 06-Apr-10 09:36 06-Apr-10 46 Within Normal Limits4553 F 4/1 06-Apr-10 10:01 06-Apr-10 200 Within Normal Limits4554 F 4/1 06-Apr-10 10:33 06-Apr-10 205 Within Normal Limits4555 F 4/1 06-Apr-10 11:24 06-Apr-10 205 Within Normal Limits4556 F 4/1 07-Apr-10 09:11 07-Apr-10 200 Within Normal Limits4557 F 4/1 07-Apr-10 09:57 07-Apr-10 362 Within Normal Limits4558 F 4/1 07-Apr-10 10:02 07-Apr-10 222 Within Normal Limits4559 F 4/1 07-Apr-10 10:20 07-Apr-10 358 Within Normal Limits4560 F 4/1 07-Apr-10 10:51 07-Apr-10 205 Within Normal Limits4561 F 4/1 07-Apr-10 11:34 07-Apr-10 222 Within Normal Limits4562 F 4/1 08-Apr-10 09:20 08-Apr-10 322 Within Normal LimitsHuntingdon Life Sciences Raw Data Listing of Gross Observations with Modifiers Printed: 26-May-10Mettlers Road Study number: 09-4348 Page: 143P.O. Box 2360Rabbit/New Zealand White Study start date: 08-Mar-10 Segment II/Inhalation___________________________________________________________________________________________________________________________________Animal Group/ Date and time Date data Oprnumber Sex Subgroup data was entered taken # Tissue / Observation(s) Locator, Color, General, Measurements,Severity levels-----------------------------------------------------------------------------------------------------------------------------------4563 F 4/1 Placenta08-Apr-10 09:34 08-Apr-10 46 Discolored, , Pale, , , Moderate#5Tissues marked 'Within Normal Limits':08-Apr-10 09:38 08-Apr-10 46 Body (entire)4564 F 4/1 08-Apr-10 10:06 08-Apr-10 200 Within Normal Limits4565 F 4/1 08-Apr-10 10:21 08-Apr-10 46 Within Normal Limits4566 F 4/1 Placenta08-Apr-10 11:01 08-Apr-10 205 Discolored, , Red, , , SeverePlacenta 108-Apr-10 11:02 08-Apr-10 205 Enlarged, , , , , SeverePlacenta 108-Apr-10 11:07 08-Apr-10 205 Discolored, , Pale, , , ModeratePlacenta # 2, 6, 7, 8.08-Apr-10 11:07 08-Apr-10 205 Small, , , , , ModeratePlacenta 6Tissues marked 'Within Normal Limits':08-Apr-10 11:11 08-Apr-10 205 Body (entire)4567 F 4/1 09-Apr-10 09:20 09-Apr-10 200 Within Normal Limits4568 F 4/1 09-Apr-10 10:00 09-Apr-10 362 Within Normal Limits4569 F 4/1 Spleen09-Apr-10 08:37 09-Apr-10 250 Small, , , , , ModerateTissues marked 'Within Normal Limits':09-Apr-10 08:37 09-Apr-10 250 Body (entire) Placenta4570 F 4/1 09-Apr-10 10:03 09-Apr-10 205 Within Normal Limits4571 F 4/1 09-Apr-10 10:51 09-Apr-10 205 Within Normal Limits4572 F 4/1 09-Apr-10 11:34 09-Apr-10 205 Within Normal LimitsHuntingdon Life Sciences09-4348Page 144MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 1 - 0 ppm1551 9 8 0 0 0 0 4 4 8 50 11 01552 9 9 0 0 0 0 3 6 9 33 0 01553 10 10 0 0 0 0 1 9 10 10 0 01554 8 8 0 0 0 0 4 4 8 50 0 01555 14 11 0 0 0 0 6 5 11 55 21 01556 10 10 0 0 0 0 4 6 10 40 0 01557 8 8 0 0 0 0 7 1 8 88 0 01558 7 7 0 0 0 0 2 5 7 29 0 01559 7 5 0 0 0 0 4 1 5 80 29 01560 10 8 0 0 0 0 5 3 8 63 20 01561 11 10 0 0 0 0 5 5 10 50 9 01562 7 7 2 0 2 0 2 3 5 40 0 291563 12 12 0 0 0 0 4 8 12 33 0 01564 12 12 1 1 2 0 5 5 10 50 0 171565 13 13 1 1 2 0 6 5 11 55 0 151566 12 12 1 0 1 0 5 6 11 45 0 81567 10 10 0 0 0 0 9 1 10 90 0 01568 9 9 0 0 0 0 4 5 9 44 0 01569 10 9 1 0 1 0 7 1 8 88 10 111570 11 10 2 0 2 0 3 5 8 38 9 201571 11 11 3 0 3 0 5 3 8 63 0 271572 10 9 0 0 0 0 3 6 9 33 10 0Impl. = ImplantationsHuntingdon Life Sciences09-4348Page 145MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 1 - 0 ppmMean 10.0 9.5 0.5 0.1 0.6 0.0 4.5 4.4 8.9 51.1 5.4 5.8SD 1.93 1.95 0.86 0.29 0.96 0.00 1.84 2.17 1.81 20.63 8.50 9.59N22 22 22 22 22 22 22 22 22 22 22 22Impl. = ImplantationsHuntingdon Life Sciences09-4348Page 146MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 2 - 2500 ppm2551 9 8 1 0 1 0 6 1 7 86 11 132552 4 4 0 0 0 0 3 1 4 75 0 02553 11 11 1 0 1 0 6 4 10 60 0 92554 9 8 0 0 0 0 5 3 8 63 11 02555 10 9 0 0 0 0 7 2 9 78 10 02556 np - - - - - - - - - - - -2557 10 9 0 0 0 0 5 4 9 56 10 02558 8 5 0 0 0 0 1 4 5 20 38 02559 6 6 0 0 0 0 1 5 6 17 0 02560 9 9 0 0 0 0 4 5 9 44 0 02561 10 9 0 0 0 0 3 6 9 33 10 02562 12 12 1 0 1 0 6 5 11 55 0 82563 9 9 0 1 1 0 4 4 8 50 0 112564 9 9 0 0 0 0 3 6 9 33 0 02565 9 9 0 1 1 0 4 4 8 50 0 112566 10 10 0 0 0 0 8 2 10 80 0 02567 10 10 0 0 0 0 3 7 10 30 0 02568 10 8 0 0 0 0 4 4 8 50 20 02569 10 10 0 0 0 0 2 8 10 20 0 02570 9 9 0 0 0 0 4 5 9 44 0 02571 10 10 0 0 0 0 4 6 10 40 0 02572 10 10 0 0 0 0 4 6 10 40 0 0Impl. = Implantations, np = not pregnantHuntingdon Life Sciences09-4348Page 147MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 2 - 2500 ppmMean 9.2 8.8 0.1 0.1 0.2 0.0 4.1 4.4 8.5 48.7 5.2 2.5SD 1.67 1.87 0.36 0.30 0.44 0.00 1.80 1.86 1.78 19.97 9.43 4.61N21 21 21 21 21 21 21 21 21 21 21 21Impl. = ImplantationsHuntingdon Life Sciences09-4348Page 148MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 3 - 10000 ppm3551 11 7 1 0 1 0 1 5 6 17 36 143552 14 13 1 0 1 0 9 3 12 75 7 83553 9 9 0 0 0 0 6 3 9 67 0 03554 9 9 0 0 0 0 4 5 9 44 0 03555 10 9 1 0 1 0 1 7 8 13 10 113556 9 9 0 0 0 0 1 8 9 11 0 03557 10 10 0 0 0 0 5 5 10 50 0 03558 7 7 0 0 0 0 4 3 7 57 0 03559 6 6 0 0 0 0 3 3 6 50 0 03560 12 8 0 0 0 0 4 4 8 50 33 03561 8 8 0 1 1 0 4 3 7 57 0 133562 11 7 0 0 0 0 5 2 7 71 36 03563 13 13 0 0 0 0 3 10 13 23 0 03564 np - - - - - - - - - - - -3565 9 6 0 0 0 0 3 3 6 50 33 03566 9 8 0 0 0 0 7 1 8 88 11 03567 9 9 1 1 2 0 4 3 7 57 0 223568 np - - - - - - - - - - - -3569 12 11 0 0 0 0 7 4 11 64 8 03570 13 10 1 0 1 0 3 6 9 33 23 103571 13 3 0 0 0 0 3 0 3 100 77 03572 9 9 0 2 2 0 4 3 7 57 0 22Impl. = Implantations, np = not pregnantHuntingdon Life Sciences09-4348Page 149MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 3 - 10000 ppmMean 10.2 8.6 0.3 0.2 0.5 0.0 4.1 4.1 8.1 51.7 13.8 5.0SD 2.16 2.33 0.44 0.52 0.69 0.00 2.06 2.35 2.29 23.69 20.33 7.71N20 20 20 20 20 20 20 20 20 20 20 20Impl. = ImplantationsHuntingdon Life Sciences09-4348Page 150MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 4 - 15000 ppm4551 12 12 1 0 1 0 8 3 11 73 0 84552 9 8 0 0 0 0 4 4 8 50 11 04553 9 8 0 0 0 0 7 1 8 88 11 04554 9 9 0 0 0 0 8 1 9 89 0 04555 11 11 2 0 2 0 4 5 9 44 0 184556 8 7 1 0 1 0 3 3 6 50 13 144557 10 10 0 1 1 0 6 3 9 67 0 104558 9 9 0 0 0 0 3 6 9 33 0 04559 10 5 0 0 0 0 3 2 5 60 50 04560 11 9 0 0 0 0 3 6 9 33 18 04561 8 7 0 1 1 0 4 2 6 67 13 144562 10 9 0 0 0 0 5 4 9 56 10 04563 12 12 0 0 0 0 6 6 12 50 0 04564 11 11 1 0 1 0 7 3 10 70 0 94565 12 11 0 0 0 0 2 9 11 18 8 04566 11 10 0 0 0 5 3 2 5 60 9 504567 11 10 0 0 0 0 1 9 10 10 9 04568 11 11 0 0 0 0 5 6 11 45 0 04569 pd 10x 10x 0x 0x 0x 0x 3x 7x 10x 30x 0x 0x4570 12 7 0 1 1 0 2 4 6 33 42 144571 10 8 0 0 0 0 3 5 8 38 20 04572 12 10 0 0 0 0 6 4 10 60 17 0Impl. = Implantations, pd = premature delivery, x = excluded from calculationHuntingdon Life Sciences09-4348Page 151MA-252E-93-23FinalReportIndividual Cesarean Section DataAppendix IAnimal Corpora Implant- Resorptions Dead Live Fetuses Sex Ratio Pre Impl. Post Impl.Number Lutea ations Early Late Total Fetuses Male Female Total (% Male) Loss (%) Loss (%)Group 4 - 15000 ppmMean 10.4 9.2 0.2 0.1 0.4 0.2 4.4 4.2 8.6 52.1 11.0 6.6SD 1.32 1.84 0.54 0.36 0.59 1.09 2.04 2.25 2.04 20.35 13.44 11.79N21 21 21 21 21 21 21 21 21 21 21 21Impl. = ImplantationsHuntingdon Life Sciences09-4348Page 152MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 1 - 0 ppm1551 3702 4051 349 487.10 3564 -1391552 3880 4137 256 521.20 3615 -2651553 3158 3337 179 563.00 2774 -3841554 3160 3570 410 501.00 3069 -911555 3362 3800 438 610.50 3190 -1731556 3171 3356 186 545.00 2811 -3591557 3141 3217 75 420.70 2796 -3461558 3460 3828 368 418.90 3409 -511559 3339 3485 146 285.00 3200 -1391560 3565 3701 136 506.30 3194 -3701561 3859 4091 232 578.20 3513 -3461562 3067 3265 197 323.93 2941 -1271563 3414 3914 501 567.60 3347 -671564 3876 4260 384 613.10 3647 -2291565 3430 3944 514 560.40 3384 -461566 3192 3626 434 623.70 3003 -1891567 3317 3693 376 492.50 3200 -1171568 3282 3646 364 532.40 3114 -1691569 3163 3475 312 461.40 3014 -1501570 3120 3310 190 449.20 2861 -2591571 3690 4079 388 512.70 3566 -1241572 3243 3678 436 525.70 3153 -90Adj = Adjusted, Bwt = BodyweightHuntingdon Life Sciences09-4348Page 153MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 1 - 0 ppmMean 3390 3703 312 504.52 3198 -192SD 263 309 127 86.445 277 110N 22 22 22 22 22 22Huntingdon Life Sciences09-4348Page 154MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 2 - 2500 ppm2551 3457 3765 308 467.90 3297 -1602552 3194 3492 298 269.00 3223 292553 3570 3586 15 537.30 3048 -5222554 3348 3288 -60 450.20 2838 -5102555 3845 3908 63 479.20 3429 -4172556 np - - - - - -2557 3760 3700 -60 434.90 3265 -4952558 3007 3168 162 327.80 2840 -1662559 3694 3881 188 396.40 3485 -2092560 3349 3726 378 567.50 3159 -1902561 3153 3517 364 520.40 2997 -1562562 3208 3688 480 553.18 3135 -732563 3462 3517 55 441.10 3076 -3862564 3274 3632 358 561.00 3071 -2032565 3730 3996 265 446.90 3549 -1812566 3591 3893 302 555.80 3337 -2542567 3375 3798 423 553.20 3245 -1302568 3620 4113 493 557.60 3555 -652569 3235 3557 321 469.10 3088 -1482570 3324 3648 324 487.90 3160 -1642571 3505 3841 336 595.90 3245 -2602572 3366 3847 480 555.90 3291 -76Adj = Adjusted, Bwt = Bodyweight, np = not pregnantHuntingdon Life Sciences09-4348Page 155MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 2 - 2500 ppmMean 3432 3693 262 487.06 3206 -226SD 222 227 172 83.754 200 154N 21 21 21 21 21 21Adj = Adjusted, Bwt = BodyweightHuntingdon Life Sciences09-4348Page 156MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 3 - 10000 ppm3551 3038 3283 245 354.20 2929 -1093552 3618 3979 361 593.00 3386 -2323553 3632 3918 286 483.90 3434 -1983554 3455 3500 45 419.30 3080 -3743555 3098 3073 -25 439.00 2634 -4643556 3094 3191 97 561.00 2630 -4643557 3204 3487 283 581.50 2905 -2983558 3620 3983 363 496.70 3486 -1343559 3646 3918 272 414.00 3504 -1423560 3677 3723 46 421.50 3301 -3753561 3461 3400 -61 479.50 2920 -5403562 3318 3835 517 410.03 3425 1063563 3228 3754 526 655.70 3098 -1303564 np - - - - - -3565 3529 3792 263 452.60 3340 -1893566 3832 3546 -286 428.60 3117 -7153567 3178 3535 357 488.70 3046 -1323568 np - - - - - -3569 2862 3206 344 533.10 2673 -1893570 4068 3978 -90 536.90 3441 -6273571 3467 3619 153 236.60 3383 -843572 3182 3572 390 390.00 3182 0Adj = Adjusted, Bwt = Bodyweight, np = not pregnantHuntingdon Life Sciences09-4348Page 157MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 3 - 10000 ppmMean 3410 3615 204 468.79 3146 -265SD 301 284 213 94.317 290 213N 20 20 20 20 20 20Adj = Adjusted, Bwt = BodyweightHuntingdon Life Sciences09-4348Page 158MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 4 - 15000 ppm4551 3757 3912 155 629.90 3282 -4754552 3181 3616 435 542.70 3073 -1084553 3259 3591 333 522.80 3069 -1904554 3263 3476 213 513.40 2963 -3004555 3783 4105 322 448.90 3657 -1274556 3215 3232 17 380.60 2852 -3644557 3490 3566 76 521.90 3044 -4464558 3283 3550 267 519.60 3031 -2534559 3621 4012 392 403.90 3609 -124560 3491 3769 278 559.70 3210 -2824561 3505 3697 192 456.40 3241 -2644562 3119 3555 436 486.90 3068 -514563 3426 3700 274 644.50 3055 -3714564 3462 3853 391 551.50 3302 -1614565 3670 3744 74 554.80 3190 -4814566 3845 3994 149 629.60 3365 -4804567 3581 3615 34 607.10 3008 -5734568 3955 4317 362 650.90 3666 -2894569 pd 3749x 3138x -610x 88.60x 3050x -699x4570 3662 3980 319 447.40 3533 -1294571 3671 4162 491 502.80 3659 -124572 3595 3861 266 563.50 3297 -297Adj = Adjusted, Bwt = Bodyweight, pd = premature delivery, x = excluded from calculationHuntingdon Life Sciences09-4348Page 159MA-252E-93-23FinalReportIndividual Gravid Uterine Adjusted Body WeightsFemales (grams) AppendixJAnimal Bodyweight on Bodyweight on Bwt change Gravid Uterine Adj Bwt Adj bwt changeNumber Day 6 Day 29 Days 6-29 Weight Day 29 Days 6-29Group 4 - 15000 ppmMean 3516 3777 261 530.42 3246 -270SD 232 260 138 76.468 251 164N 21 21 21 21 21 21Adj = Adjusted, Bwt = Bodyweight

# Huntingdon Life Sciences 09-4348 Page 160

MA-252E-93-23 Final Report

Individual Litter Weight Data

Preface Appendix K

Individual Litter Fetal and Placental Weights ................................................................. 161

Individual Fetal Weights .................................................................................................. 169

Individual Placental Weights ........................................................................................... 177Huntingdon Life Sciences09-4348Page 161MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 1 – 0 ppm1551 5.0 338.5 41.6 43.0 42.31552 5.3 362.1 41.0 39.8 40.21553 5.6 394.9 33.8 40.1 39.51554 4.6 353.4 44.1 44.3 44.21555 5.2 421.2 39.5 36.8 38.31556 4.5 372.2 37.1 37.3 37.21557 4.5 293.5 37.1 33.9 36.71558 5.4 301.7 46.4 41.8 43.11559 3.9 180.6 34.9 40.9 36.11560 5.1 371.8 45.8 47.6 46.51561 4.8 399.7 38.5 41.4 40.01562 4.5 213.7 43.6 42.2 42.71563 3.9 391.2 34.6 31.6 32.61564 4.8 388.4 42.2 35.5 38.81565 4.5 362.9 32.5 33.6 33.01566 4.5 436.9 43.5 36.6 39.71567 4.1 331.6 34.2 23.9 33.21568 4.4 360.6 39.0 40.9 40.11569 5.4 321.0 41.6 29.7 40.11570 4.8 298.1 37.3 37.2 37.31571 5.6 343.2 43.7 41.6 42.91572 4.5 368.4 42.4 40.2 40.9Huntingdon Life Sciences09-4348Page 162MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 1 – 0 ppmMean 4.8 345.7 39.7 38.2 39.3SD 0.51 61.12 4.11 5.34 3.64N 22 22 22 22 22Huntingdon Life Sciences09-4348Page 163MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 2 – 2500 ppm2551 5.3 308.0 43.3 48.4 44.02552 5.3 175.1 44.5 41.8 43.82553 4.3 380.7 37.7 38.6 38.12554 5.6 297.1 37.3 36.8 37.12555 4.7 304.1 33.5 34.7 33.82557 3.9 293.6 35.3 29.3 32.62558 5.4 216.4 44.5 43.0 43.32559 4.8 268.2 45.3 44.6 44.72560 5.8 379.6 43.6 41.0 42.22561 4.7 361.0 39.9 40.2 40.12562 4.6 397.3 36.1 36.2 36.12563 4.6 282.6 39.3 31.3 35.32564 4.9 382.5 43.0 42.2 42.52565 4.9 303.6 40.5 35.4 38.02566 4.9 389.7 38.3 41.6 39.02567 4.1 383.8 40.3 37.6 38.42568 5.8 376.8 47.5 46.7 47.12569 4.1 323.0 32.7 32.2 32.32570 5.2 331.1 34.9 38.3 36.82571 4.6 412.3 39.3 42.5 41.22572 5.2 371.8 35.9 38.0 37.2Huntingdon Life Sciences09-4348Page 164MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 2 – 2500 ppmMean 4.9 330.4 39.7 39.1 39.2SD 0.54 62.33 4.16 4.92 4.13N 21 21 21 21 21Huntingdon Life Sciences09-4348Page 165MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 3 – 10000 ppm3551 4.9 252.2 45.5 41.3 42.03552 5.0 418.7 34.7 35.6 34.93553 5.0 326.2 36.5 35.7 36.23554 4.1 267.6 28.4 30.8 29.73555 4.9 306.1 41.5 37.8 38.33556 4.9 402.3 46.5 44.5 44.73557 4.6 400.7 39.6 40.6 40.13558 6.5 321.9 45.0 47.4 46.03559 5.5 268.8 46.7 42.9 44.83560 4.5 276.8 34.0 35.2 34.63561 4.2 296.4 42.0 42.8 42.33562 4.7 280.0 38.4 44.0 40.03563 4.6 459.8 34.4 35.7 35.43565 6.8 309.9 51.5 51.8 51.73566 5.0 274.5 34.4 33.8 34.33567 5.5 322.6 45.8 46.4 46.13569 4.4 381.3 36.3 31.7 34.73570 5.0 342.2 39.9 37.1 38.03571 6.4 139.6 46.5 - 46.53572 4.7 234.5 32.4 35.0 33.5Huntingdon Life Sciences09-4348Page 166MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 3 – 10000 ppmMean 5.1 314.1 40.0 39.5 39.7SD 0.74 73.41 6.10 5.74 5.67N 20 20 20 19 20Huntingdon Life Sciences09-4348Page 167MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 4 – 15000 ppm4551 5.1 414.1 36.5 40.8 37.64552 5.9 354.0 44.6 43.9 44.24553 5.1 353.5 43.8 46.9 44.24554 4.7 378.1 42.0 41.9 42.04555 4.4 327.0 36.7 36.1 36.34556 4.8 270.1 46.2 43.8 45.04557 4.6 325.4 36.5 35.5 36.24558 4.9 367.1 47.8 37.3 40.84559 6.5 264.3 54.6 50.2 52.94560 5.0 390.1 45.2 42.4 43.34561 5.1 281.0 46.1 48.2 46.84562 5.3 334.5 39.8 33.9 37.24563 5.1 452.5 39.7 35.7 37.74564 4.6 378.0 37.9 37.5 37.84565 4.3 395.0 36.3 35.8 35.94566 5.8 389.9 40.7 36.5 39.04567 5.2 393.4 38.9 39.4 39.34568 5.1 438.3 42.8 37.4 39.84569 pd 4.3x 306.7x 30.7x 30.6x 30.7x4570 5.4 266.4 48.7 42.2 44.44571 5.1 342.4 42.2 43.1 42.84572 4.3 378.3 39.0 36.0 37.8pd = premature delivery, x = excluded from calculationsHuntingdon Life Sciences09-4348Page 168MA-252E-93-23FinalReportIndividual Litter Fetal and Placental Weights(grams) AppendixKAnimal Placental Weight Total Litter Male Fetal Weight Female Fetal Weight Overall Fetal WeightNumber Mean Weight Mean Mean MeanGroup 4 – 15000 ppmMean 5.1 356.8 42.2 40.2 41.0SD 0.54 53.92 4.80 4.65 4.30N 21 21 21 21 21Huntingdon Life Sciences09-4348Page 169MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 – 0 ppm1551 42.3 43.9 41.8 42.9 44.7 41.6 40.2 40.8 42.51552 40.2 45.7 43.0 39.4 34.2 38.7 43.3 35.6 36.3 45.91553 39.5 43.8 40.5 33.8 28.3 40.9 39.0 35.5 42.5 41.3 49.21554 44.2 47.1 46.2 43.1 40.7 42.4 45.5 43.5 44.91555 38.3 37.6 42.3 33.2 38.4 37.0 32.841.1 34.6 42.8 41.5 40.11556 37.2 40.2 33.2 36.1 36.4 37.2 37.6 36.4 36.1 39.0 40.11557 36.7 39.1 34.2 38.1 33.9 36.7 36.3 34.9 40.41558 43.1 46.9 46.5 40.2 41.2 41.0 39.6 46.31559 36.1 35.5 33.0 35.4 35.9 40.91560 46.5 44.1 48.7 45.5 55.9 42.1 43.4 43.5 48.61561 40.0 48.8 45.9 44.1 39.1 30.2 29.7 34.2 40.3 42.4 44.91562 42.8 40.5 44.4 42.8 40.7 45.41563 32.6 36.0 28.8 30.4 26.6 23.0 26.331.2 30.5 34.8 37.6 42.0 44.01564 38.8 47.5 38.5 41.4 29.9 23.2 41.4 43.3 38.2 42.7 42.31565 33.0 45.7 38.7 24.2 25.2 23.7 35.932.5 25.4 27.5 37.6 46.61566 39.7 47.2 43.9 41.2 38.7 34.0 32.733.4 43.8 37.0 36.6 48.51567 33.2 37.1 37.2 29.7 31.1 27.8 23.9 30.8 39.8 37.6 36.51568 40.1 42.1 44.3 41.7 39.9 29.2 40.3 39.8 40.6 42.61569 40.1 48.2 40.4 38.0 30.7 29.7 39.7 43.9 50.41570 37.3 37.0 38.0 42.2 40.5 29.2 35.5 35.3 40.51571 42.9 46.5 47.2 44.9 40.6 36.5 39.0 43.2 45.31572 40.9 42.8 40.6 40.3 40.4 32.8 38.5 40.0 46.2 46.8Huntingdon Life Sciences09-4348Page 170MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 – 0 ppmMean 39.3SD 3.64N 22Huntingdon Life Sciences09-4348Page 171MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 2 – 2500 ppm2551 44.0 48.4 45.9 42.6 44.4 42.7 37.7 46.32552 43.8 41.8 40.1 48.1 45.22553 38.0 36.2 34.2 34.9 38.4 39.3 37.5 40.2 38.2 39.8 41.72554 37.2 42.0 26.9 41.4 30.1 36.7 39.7 38.1 42.32555 33.8 39.5 31.3 32.3 30.9 30.5 32.2 29.0 37.2 41.22557 32.6 40.6 34.4 27.7 22.5 30.3 34.7 30.1 36.4 36.92558 43.3 44.3 41.6 41.7 44.3 44.52559 44.7 43.6 41.5 44.8 45.3 48.2 44.92560 42.2 49.3 46.7 43.5 42.1 38.6 39.2 39.9 49.3 31.12561 40.1 42.1 41.5 41.1 36.1 40.3 39.6 33.8 39.2 47.32562 36.1 43.6 33.9 33.7 35.2 33.1 41.726.5 28.7 37.5 39.7 43.62563 35.3 34.8 46.5 39.4 32.1 24.3 26.7 34.4 44.32564 42.5 45.3 48.3 49.5 42.6 37.8 38.8 36.9 40.7 42.52565 38.0 33.8 36.4 41.2 31.6 37.7 41.0 39.7 42.22566 39.0 44.4 44.1 41.0 33.0 32.4 29.7 36.6 42.7 42.1 43.72567 38.4 50.0 40.3 41.3 34.1 30.9 31.3 39.9 44.2 39.6 32.12568 47.1 53.7 48.4 48.0 46.1 40.0 43.2 47.3 50.12569 32.3 35.0 34.4 34.3 30.5 29.4 27.2 28.6 31.9 35.6 36.02570 36.8 40.1 42.0 38.1 30.7 38.8 36.1 32.5 35.0 37.62571 41.2 47.7 39.7 48.8 37.6 37.7 37.9 37.5 42.6 43.9 38.92572 37.2 39.4 38.2 38.1 33.8 34.3 30.8 33.7 36.9 43.8 42.9Huntingdon Life Sciences09-4348Page 172MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 2 – 2500 ppmMean 39.2SD 4.13N 21Huntingdon Life Sciences09-4348Page 173MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 – 10000 ppm3551 42.1 45.4 41.0 45.5 38.5 37.3 44.63552 34.9 42.1 38.4 37.0 36.3 32.8 25.934.8 32.0 23.3 28.2 39.1 48.73553 36.2 39.2 37.2 35.8 35.6 34.9 32.5 37.2 37.0 36.73554 29.7 31.6 32.6 35.7 27.5 13.9 26.4 31.8 34.9 33.23555 38.3 43.9 33.7 39.8 36.0 33.3 38.8 41.5 39.03556 44.7 48.3 43.4 44.2 45.3 45.0 38.3 44.5 46.5 46.73557 40.1 44.8 41.8 36.3 34.8 36.5 36.4 40.7 43.6 42.6 43.23558 46.0 52.3 45.0 54.1 35.6 41.4 46.7 46.93559 44.8 52.1 40.6 40.5 39.7 48.3 47.63560 34.6 36.4 32.1 35.8 30.2 35.7 33.5 33.2 39.93561 42.3 45.8 43.1 39.4 39.9 40.1 42.6 45.53562 40.0 35.0 36.8 39.5 40.8 39.8 44.7 43.43563 35.4 44.0 37.6 30.0 30.4 33.7 26.3 26.3 35.3 40.4 41.9 40.0 32.8 41.23565 51.7 54.2 50.5 50.9 49.5 50.5 54.33566 34.3 38.0 33.8 33.1 34.2 31.4 30.1 35.6 38.33567 46.1 47.7 44.8 40.8 47.6 48.2 43.1 50.33569 34.7 39.0 37.2 35.0 31.3 30.5 23.832.8 33.4 35.9 37.3 45.23570 38.0 33.0 41.1 41.9 40.8 39.5 33.1 29.6 44.8 38.53571 46.6 46.6 48.5 44.63572 33.5 36.3 40.1 30.0 34.5 30.4 24.2 39.1Huntingdon Life Sciences09-4348Page 174MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 – 10000 ppmMean 39.7SD 5.67N 20Huntingdon Life Sciences09-4348Page 175MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 – 15000 ppm4551 37.7 47.2 29.8 34.1 37.6 32.8 31.133.1 38.3 40.9 42.9 46.44552 44.2 44.6 43.8 42.7 43.9 47.1 41.7 43.3 46.84553 44.2 48.5 35.6 36.8 43.9 45.9 48.3 46.9 47.64554 42.0 47.2 43.4 36.7 41.4 37.5 38.1 42.5 41.9 49.44555 36.3 38.4 39.0 39.2 37.7 30.9 35.4 29.0 33.1 44.34556 45.0 43.5 47.3 42.9 45.1 46.1 45.24557 36.2 36.1 35.1 35.0 37.4 34.5 34.5 34.4 38.8 39.74558 40.8 47.8 42.0 38.8 35.9 30.4 37.5 45.8 39.2 49.84559 52.9 50.5 50.0 54.2 53.4 56.24560 43.3 43.5 46.7 48.0 43.7 44.9 37.0 37.7 42.8 45.84561 46.9 50.9 45.5 45.6 39.8 49.2 50.14562 37.2 42.6 40.2 38.5 33.9 33.4 33.3 35.0 36.4 41.24563 37.7 43.9 38.9 42.0 39.3 35.0 33.331.7 28.6 35.1 36.1 44.8 43.94564 37.8 47.7 41.9 39.9 38.2 28.9 30.6 33.4 37.3 39.1 40.94565 35.9 44.0 34.7 32.6 26.2 37.7 39.133.5 34.1 39.8 46.6 26.74566 39.0 41.7 47.9 42.0 43.0 33.3 27.2 40.9 33.7 37.9 42.44567 39.3 37.9 36.3 44.8 42.4 38.9 43.8 37.1 28.4 38.5 45.34568 39.9 49.9 47.4 40.7 32.8 33.9 30.034.7 37.7 44.3 44.4 42.64569 pd 30.7x 42.5x 33.3x 21.6x 35.0x 39.0x 24.8x 30.4x 21.3x 29.8x 29.1x4570 44.4 51.8 42.8 45.7 39.0 45.8 41.34571 42.8 47.5 45.8 42.7 40.6 37.7 40.5 43.3 44.44572 37.8 45.2 41.9 36.3 35.6 32.3 32.1 34.3 36.9 40.2 43.5pd = premature delivery, x = excluded from calculationsHuntingdon Life Sciences09-4348Page 176MA-252E-93-23FinalReportIndividual Fetal Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 – 15000 ppmMean 41.0SD 4.30N 21Huntingdon Life Sciences09-4348Page 177MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 – 0 ppm1551 5.0 5.1 5.2 5.2 4.7 5.1 5.1 4.5 5.31552 5.3 5.9 6.0 5.0 4.1 5.5 4.9 4.6 5.0 7.01553 5.6 6.3 5.5 6.8 4.2 5.2 5.2 5.0 5.6 5.7 6.11554 4.6 5.9 5.0 3.9 4.2 4.3 4.6 4.2 4.51555 5.1 5.4 5.5 4.5 5.1 4.5 4.1 5.0 4.4 5.4 6.7 6.01556 4.5 4.4 4.0 4.9 4.3 4.1 4.1 4.2 5.0 5.3 4.91557 4.6 4.7 4.4 4.7 3.8 4.3 4.7 4.1 5.71558 5.4 5.3 6.4 4.9 4.8 4.8 5.2 6.21559 3.9 3.8 3.6 3.5 4.1 4.51560 5.1 6.4 5.7 4.9 5.7 4.6 4.3 4.5 5.01561 4.8 6.4 5.2 5.3 4.7 4.1 3.7 3.9 4.5 5.0 5.41562 4.6 4.6 4.9 4.7 3.9 4.71563 3.9 4.2 3.4 3.7 3.0 2.6 3.4 4.2 4.2 3.6 4.0 5.2 5.31564 4.8 5.6 4.8 5.9 3.7 3.2 5.0 5.1 4.5 5.4 5.01565 4.5 6.4 5.6 3.8 3.4 3.7 4.5 4.5 3.8 3.1 4.9 5.81566 4.5 5.3 5.1 4.6 4.5 3.6 3.5 3.8 5.3 4.0 3.7 5.71567 4.1 4.8 4.9 3.2 3.4 3.5 2.8 3.6 5.3 4.3 4.81568 4.4 4.5 5.4 4.5 3.9 3.1 4.5 4.6 4.7 4.61569 5.4 6.4 6.1 4.6 4.5 3.6 5.4 6.1 6.21570 4.8 4.4 5.1 5.4 4.7 3.3 4.4 5.3 5.71571 5.6 6.2 6.4 6.4 5.3 4.4 5.3 5.4 5.71572 4.5 4.8 4.5 3.9 4.6 3.9 4.3 4.4 5.1 4.7Huntingdon Life Sciences09-4348Page 178MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 1 – 0 ppmMean 4.8SD 0.50N 22Huntingdon Life Sciences09-4348Page 179MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 2 – 2500 ppm2551 5.3 5.8 5.9 5.0 4.6 5.5 4.7 5.72552 5.3 4.7 4.6 6.1 5.62553 4.3 4.1 4.5 4.2 4.1 4.5 4.1 4.5 4.3 4.3 4.72554 5.6 6.1 4.9 6.3 4.5 5.3 5.6 5.8 6.32555 4.7 6.1 4.1 5.1 4.4 4.9 4.5 4.2 4.2 5.22557 3.9 5.6 3.7 3.0 2.7 3.7 3.9 3.5 4.5 4.12558 5.4 5.4 4.9 4.9 5.4 6.32559 4.8 4.5 4.7 5.0 4.5 5.1 4.72560 5.8 6.7 7.0 6.0 5.9 4.5 4.9 5.5 6.6 5.52561 4.7 5.0 4.8 4.7 4.7 5.1 4.3 4.2 4.9 5.02562 4.6 5.5 4.9 4.2 4.8 4.0 5.3 3.7 3.7 4.8 4.4 5.22563 4.6 5.7 5.9 4.7 4.2 3.1 3.1 4.3 5.92564 4.9 4.5 6.0 6.2 5.1 4.1 4.7 3.9 4.8 4.82565 4.9 4.0 4.8 5.4 3.9 4.9 4.9 4.9 6.22566 4.9 5.1 5.5 5.2 4.0 4.4 4.0 4.3 5.5 5.3 5.62567 4.1 5.0 4.9 4.5 3.3 3.2 3.2 4.3 4.4 4.2 4.42568 5.8 7.2 5.9 5.7 5.6 5.1 5.5 5.5 6.02569 4.1 4.3 4.2 3.9 3.9 3.5 3.7 3.9 3.7 4.3 5.22570 5.2 6.2 5.4 4.8 4.2 5.1 5.1 5.1 4.6 6.42571 4.6 5.2 4.3 4.9 4.2 4.2 4.2 4.1 4.6 5.2 5.22572 5.2 5.6 5.1 5.2 4.7 5.0 4.0 4.8 5.8 5.8 6.1Huntingdon Life Sciences09-4348Page 180MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 2 – 2500 ppmMean 4.9SD 0.55N 21Huntingdon Life Sciences09-4348Page 181MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 – 10000 ppm3551 4.9 4.7 6.0 5.0 4.6 4.2 4.83552 5.0 5.6 5.3 5.4 4.7 4.5 4.2 4.7 4.4 3.7 4.3 6.1 7.33553 5.0 5.5 5.2 5.0 4.1 4.3 5.1 5.2 5.2 5.03554 4.1 4.4 4.6 4.6 3.8 2.4 3.3 4.5 4.6 4.93555 4.9 5.4 5.1 5.2 4.7 3.9 4.8 5.6 4.83556 4.9 5.6 4.9 4.6 4.9 4.6 4.2 5.2 5.1 5.33557 4.6 5.6 5.4 4.3 3.7 4.1 3.5 4.5 4.9 5.1 4.93558 6.5 7.0 7.0 6.6 6.4 5.9 6.4 6.03559 5.5 6.0 5.0 4.8 5.4 5.6 5.93560 4.5 4.9 4.0 4.3 3.9 4.4 5.1 4.1 5.43561 4.2 4.5 4.4 3.7 4.3 4.0 3.9 4.93562 4.7 4.3 4.3 4.6 5.3 5.0 4.7 5.03563 4.6 5.8 4.1 3.5 3.8 4.2 3.8 4.3 4.1 5.1 5.0 5.5 5.5 5.13565 6.8 7.3 6.2 6.9 6.3 6.6 7.33566 5.0 5.3 5.2 5.1 4.9 4.1 4.9 4.8 5.43567 5.5 6.0 5.4 4.8 5.3 5.1 5.5 6.33569 4.4 4.9 4.9 4.5 4.0 3.8 2.9 4.4 4.3 4.4 4.4 5.43570 5.0 4.6 5.8 5.9 5.0 5.2 4.1 3.7 6.1 4.53571 6.4 6.4 6.8 6.13572 4.7 5.4 5.3 3.8 5.0 4.2 3.7 5.6Huntingdon Life Sciences09-4348Page 182MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 3 – 10000 ppmMean 5.1SD 0.73N 20Huntingdon Life Sciences09-4348Page 183MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 – 15000 ppm4551 5.1 6.6 4.8 4.9 4.7 4.6 4.3 4.0 4.5 6.3 5.9 5.74552 5.9 5.9 6.0 6.2 6.0 6.1 5.5 4.9 6.24553 5.1 5.7 4.0 4.0 5.2 5.3 5.9 4.6 5.84554 4.7 5.2 4.7 4.3 4.7 3.9 4.5 5.0 4.6 5.14555 4.4 4.8 4.5 4.7 4.7 3.5 4.2 3.8 4.2 5.64556 4.8 4.8 5.2 4.4 4.7 4.6 4.94557 4.6 4.9 4.5 5.0 4.4 4.5 4.4 4.0 4.6 4.94558 4.9 5.8 4.9 4.9 4.1 4.4 4.5 5.0 4.6 6.04559 6.5 5.9 5.1 8.5 6.8 6.34560 5.0 5.1 4.6 5.1 5.0 5.4 4.7 4.5 5.0 5.34561 5.1 5.6 4.7 4.7 4.8 4.8 5.74562 5.3 6.5 5.5 5.7 4.7 5.1 4.4 4.9 5.2 5.74563 5.1 6.3 4.9 5.8 5.4 4.4 4.0 4.3 3.7 4.8 4.7 6.7 6.34564 4.7 6.8 4.5 5.3 4.4 2.8 3.5 4.3 4.8 4.7 5.54565 4.3 4.9 4.2 4.0 3.0 4.6 4.7 4.3 4.1 5.0 5.6 3.34566 5.9 15.3 5.7 5.4 4.8 4.3 1.9 4.8 4.7 5.5 6.14567 5.2 4.9 4.5 6.0 5.2 5.5 5.7 4.4 3.6 5.3 6.54568 5.1 6.6 5.6 5.1 4.5 4.4 4.2 4.5 4.6 5.5 5.6 5.84569 pd 3.9x 3.9x 3.9x 2.5x 5.7x 5.5x 5.0x 4.9x 1.7x 5.5x miss4570 5.4 5.9 4.9 5.7 4.9 5.9 5.34571 5.1 5.6 5.2 5.5 5.0 4.6 4.5 5.1 5.54572 4.3 4.7 4.9 4.3 3.8 3.5 3.7 3.9 4.7 4.6 5.3pd = premature delivery, x = excluded from calculations, miss = missing at time of examinationHuntingdon Life Sciences09-4348Page 184MA-252E-93-23FinalReportIndividual Placental Weights(grams) AppendixKAnimal Litter FetusNumber Mean 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Group 4 – 15000 ppmMean 5.1SD 0.53N 21

# Huntingdon Life Sciences 09-4348 Page 185

MA-252E-93-23 Final Report

Individual Fetal Observations Appendix L

Key to Abbreviations

av = atrio-ventricular

vert. = vertebra(e)

vs = visceral/skeletal evaluationsHuntingdon Life Sciences09-4348Page 186MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1551 8vs 1 F 43.9 vs Variants: 13th bilateral rib, complete2 M 41.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral, unossifiedSuture/fontanel: frontal suture, plaque of bone3 M 42.9 vsNo abnormality detected4 F 44.7 vs Variants: 13th bilateral rib, complete5 M 41.6 vs Variants: 13th left rib, short6 M 40.2 vsNo abnormality detected7 F 40.8 vs Variants: 13th right rib, short8 F 42.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete1552 9vs 1 F 45.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, short2 M 43.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified3 F 39.4 vsLimb bones: metacarpal, 1st digit, right, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified4 M 34.2 vsLimb bones: epiphyses,incompletely ossified5 F 38.7 vsNo abnormality detected6 F 43.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified7 F 35.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedHuntingdon Life Sciences09-4348Page 187MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1552 9vs 7 F 35.6 vs Thorax: subclavian artery, left side, additionalVariants: 13th bilateral rib, complete8 F 36.3 vs Thorax: subclavian artery, branching variation, left side9 M 45.9 vsNo abnormality detected1553 10vs 1 F 43.8 vs Variants: 13th bilateral rib, short2 F 40.5 vs Thorax: common carotid artery, displaced, left sideVariants: 13th left rib, completeVariants: 13th right rib, short3 M 33.8 vsVariants: sternebra(e), 6th, unossified4 F 28.3 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossified5 F 40.9 vsNo abnormality detected6 F 39.0 vsVariants: sternebra(e), 6th, unossified7 F 35.5 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossified8 F 42.5 vsNo abnormality detected9 F 41.3 vs Variants:13th bilateral rib, short10 F 49.2 vs Variants:13th left rib, completeVariants: 13thright rib, rudimentaryVariants: sternebra(e), 6th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 188MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1554 8vs 1 M 47.1 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete2 M 46.2 vs Suture/fontanel: frontal suture, plaque of boneVariants: 13th bilateral rib, complete3 F 43.1 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete4 M 40.7 vs Variants: 13th left rib, completeVariants: 13th right rib, short5 M 42.4 vs Girdles: pelvic girdle, right, unilateral caudal shiftVariants: 13th bilateral rib, complete6 F 45.5 vs Variants:13th right rib, completeVariants: sternebra(e), 5th, incompletely ossified7 F 43.5 vsNo abnormality detected8 F 44.9 vs Suture/fontanel: frontal suture, plaque of boneVariants: 13th bilateral rib, complete1555 11vs 1 M 37.6 vs LUMBAR VERT.: LUMBAR REGION, SCOLIOSISLUMBAR VERT.: LUMBAR VERTEBRAL ARCH(ES), 1ST, RIGHT, ABSENTLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossifiedLumbar vert.: lumbar vertebral arch(es), 2nd-3rd, right, misalignedHuntingdon Life Sciences09-4348Page 189MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1555 11vs 1 M 37.6 vs Lumbar vert.: lumbar vertebral centrum(a), 1st-2nd, misalignedVariants: 13thright rib, completeVariants: sternebra(e), 5th, incompletely ossified2 M 42.3 vs Head: hyoid cornua, right, bent inwardsVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified3 F 33.2 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete4 F 38.4 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossified5 F 37.0 vsLimb bones: metacarpal, 1st digit, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified6 F 32.8 vs Variants:13th right rib, complete7 M 41.1 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedSternebrae: sternebral center(s), 6th, bipartite ossifiedVariants: 13th left rib, shortVariants: 13thright rib, completeHuntingdon Life Sciences09-4348Page 190MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1555 11vs 8 M 34.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossified9 F 42.8 vs Head: hyoid cornua, bilateral, bent inwardsVariants: 13th left rib, completeVariants: 13th right rib, short10 M 41.5 vs Head: parietal, left, unossified area(s)Limb bones: epiphyses,incompletely ossifiedLiver: gallbladder, rudimentaryLungs: accessory lung lobe, absentSternebrae: sternebral center(s), 1st, supernumerary ossificationVariants: 13th right rib, short11 M 40.1 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentThorax: common carotid artery, displaced, left sideVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified1556 10vs 1 M 40.2 vs Thorax: subclavian artery, branching variation, left side2 M 33.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified3 F 36.1 vsLimb bones: metacarpal, 1st digit, left, incompletely ossifiedHuntingdon Life Sciences09-4348Page 191MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1556 10vs 3 F 36.1 vsLimb bones: epiphyses,incompletely ossified4 F 36.4 vs Thorax: subclavian artery, left side, additional5 F 37.2 vs Thorax: common carotid artery, displaced, left sideThorax: subclavian artery, branching variation, left sideVariants: 13th left rib, rudimentary, non-articulatedVariants: 13th right rib, short6 F 37.6 vs Variants: 13th right rib, short7 F 36.4 vs Thorax: subclavian artery, left side, additional8 M 36.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThorax: common carotid artery, displaced, left sideVariants: 13thright rib, complete9 M 39.0 vs Variants: 13th right rib, short, non-articulated10 F 40.1 vsVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified1557 8vs 1 M 39.1 vs Variants: 13th bilateral rib, complete2 M 34.2 vs Head: frontal, right, unossified area(s)Head: parietal, right, unossified area(s)Limb bones: epiphyses,incompletely ossified3 M 38.1 vs Head: parietal, right, unossified area(s)Variants: 13thright rib, complete4 F 33.9 vs Head: frontal, right, unossified area(s)Variants: 13th bilateral rib, complete5 M 36.7 vsCervical vert.: odontoid process, incompletely ossifiedHuntingdon Life Sciences09-4348Page 192MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1557 8vs 5 M 36.7 vs Variants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified6 M 36.3 vs Head: parietal, right, unossified area(s)Limb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete7 M 34.9 vs Variants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 6th, incompletely ossified8 M 40.4 vs Variants: 13th bilateral rib, complete1558 7vs 1 F 46.9 vs Variants: 13th bilateral rib, short2 M 46.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified3 F 40.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short4 F 41.2 vs Variants:13th bilateral rib, short5 F 41.0 vs Liver:gallbladder,rudimentaryVariants: 13thbilateral rib, short6 F 39.6 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedVariants: 13th left rib, rudimentaryVariants: 13th right rib, short7 M 46.3 vs Head: frontal, left, unossified area(s)Variants: 13th left rib, shortHuntingdon Life Sciences09-4348Page 193MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1559 5vs 1 M 35.5 vs Variants: 13th bilateral rib, complete2 M 33.0 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossified3 M 35.4 vsLimb bones: epiphyses,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete4 M 35.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossified5 F 40.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete1560 8vs 1 M 44.1 vs Variants: 13th bilateral rib, complete2 M 48.7 vs Variants: 13th bilateral rib, complete3 M 45.5 vs Girdles:clavicle, bilateral, bentHead: interparietal,supraoccipital, partially fusedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete4 F 55.9 vs Variants: 13th bilateral rib, complete5 M 42.1 vs Variants: 13th left rib, shortVariants: 13thright rib, completeHuntingdon Life Sciences09-4348Page 194MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1560 8vs 6 F 43.4 vs Girdles: clavicle, right, bentVariants: 13th left rib, shortVariants: 13th right rib, rudimentary, non-articulated7 F 43.5 vs Variants: 13th bilateral rib, complete8 M 48.6 vs Variants: 13th bilateral rib, complete1561 10vs 1 F 48.8 vsHead: anterior supraorbital notch,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete2 F 45.9 vs Sternebrae: sternebral center(s), 3rd-4th, bridge of ossificationSternebrae: sternebral center(s), 4th-5th, bridge of ossification3 M 44.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified4 M 39.1 vsHead: anterior supraorbital notch,bilateral, incompletely ossifiedSternebrae: sternebral center(s), 4th-5th, bridge of ossificationVariants: 13thbilateral rib, short5 M 30.2 vsHead: anterior supraorbital notch,bilateral, incompletely ossifiedLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: talus,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified6 F 29.7 vs Brain: lateral ventricle, bilateral, dilatedLimb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: talus,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedHuntingdon Life Sciences09-4348Page 195MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1561 10vs 6 F 29.7 vs Sternebrae: sternebral center(s), 4th-5th,bridge of ossificationVariants: 13th left rib, completeVariants: 13th right rib, short7 M 34.2 vsHead: anterior supraorbital notch,right, incompletely ossified8 F 40.3 vsHead: anterior supraorbital notch,left, incompletely ossifiedSternebrae: sternebral center(s), 3rd-4th, bridge of ossificationSternebrae: sternebral center(s), 4th-5th, fused9 F 42.4 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSternebrae: sternebral center(s), 3rd-4th, bridge of ossificationSternebrae: sternebral center(s), 4th-5th, bridge of ossification10 M 44.9 vsHead: anterior supraorbital notch,bilateral, incompletely ossifiedLiver: gallbladder, rudimentarySuture/fontanel: frontal suture, plaque of bone1562 5vs 1 F 40.5 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThorax: subclavian artery, left side, additionalVariants: 13th bilateral rib, complete2 M 44.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete3 M 42.8 vs Head: hyoid cornua, left, bent inwardsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, rightforelimb, unossifiedHuntingdon Life Sciences09-4348Page 196MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1562 5vs 3 M 42.8 vs Variants: 13th bilateral rib, complete4 F 40.7 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete5 F 45.4 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete1563 12vs 1 F 36.0 vs Variants: 13th left rib, shortVariants: 13thright rib, rudimentary2 F 28.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified3 F 30.4 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, unossified4 F 26.6 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified5 F 23.0 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedHuntingdon Life Sciences09-4348Page 197MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1563 12vs 5 F 23.0 vsLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, unossified6 F 26.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified7 M 31.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedThorax: subclavian artery, branching variation, left sideVariants: 13th right rib, short8 M 30.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThorax: subclavian artery, branching variation, left sideVariants: sternebra(e), 5th, unossified9 M 34.8 vs Cervical: thymus gland, partially undescended, left sideLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified10 F 37.6 vs Thorax: subclavian artery, left side, additionalVariants: sternebra(e), 5th, unossified11 M 42.0 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified12 F 44.0 vsVariants: sternebra(e), 5th, unossified1564 10vs 1 M 47.5 vs Variants: 13th right rib, short2 F 38.5 vs Girdles:pelvicgirdle, left, unilateral caudal shiftLimb bones: epiphyses, unossifiedVariants: 13th bilateral rib, complete3 M 41.4 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 198MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1564 10vs 3 M 41.4 vs Variants: 13th bilateral rib, complete4 F 29.9 vs Girdles: pelvic girdle, right, unilateral cranial shiftVariants: 13th bilateral rib, complete5 F 23.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified6 M 41.4 vsSternebrae: omosternum, left, ossified7 F 43.3 vs Variants:13th bilateral rib, shortVariants: sternebra(e), 5th, unossified8 M 38.2 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13thbilateral rib, short9 F 42.7 vs Head:frontal, left, unossified area(s)Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete10 M 42.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified1565 11vs 1 M 45.7 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified2 M 38.7 vsNo abnormality detected3 M 24.2 vs Head: hyoid cornua, bilateral, bent inwardsGirdles: pubis, bilateral, incompletely ossifiedHuntingdon Life Sciences09-4348Page 199MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1565 11vs 3 M 24.2 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: anterior fontanelle, enlargedSuture/fontanel: posterior fontanelle, enlarged4 M 25.2 vs Cervical vert.: cervicalrib, right, without costal cartilageLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses, unossifiedVariants: 13th leftrib, short, non-articulated5 M 23.7 vs Girdles: pelvic girdle, right, unilateral caudal shiftLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossifiedSuture/fontanel: posterior fontanelle, enlargedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, incompletely ossified6 F 35.9 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short, non-articulatedVariants: sternebra(e), 6th, incompletely ossified7 F 32.5 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedHuntingdon Life Sciences09-4348Page 200MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1565 11vs 7 F 32.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified8 F 25.4 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified9 F 27.5 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified10 M 37.6 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13thbilateral rib, short11 F 46.6 vsVariants: sternebra(e), 5th, incompletely ossified1566 11vs 1 M 47.2 vsNo abnormality detected2 M 43.9 vs Thorax: subclavian artery, left side, additionalVariants: sternebra(e), 5th, unossified3 F 41.2 vs Variants: 13th bilateral rib, complete4 F 38.7 vs Lungs: accessory lung lobe, small5 M 34.0 vs Variants: 13th left rib, shortVariants: 13thright rib, completeHuntingdon Life Sciences09-4348Page 201MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1566 11vs 6 F 32.7 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified7 F 33.4 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified8 M 43.8 vs Variants: 13th bilateral rib, complete9 F 37.0 vs Variants: 13th bilateral rib, complete10 F 36.6 vs Lungs:accessory lung lobe, rudimentaryVariants: sternebra(e), 5th, incompletely ossified11 M 48.5 vs Variants: 13th bilateral rib, complete1567 10vs 1 M 37.1 vs Variants: 13th bilateral rib, complete2 M 37.2 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short3 M 29.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedVariants: 13th left rib, completeVariants: 13th right rib, short4 M 31.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedHuntingdon Life Sciences09-4348Page 202MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1567 10vs 4 M 31.1 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete5 M 27.8 vs Head: frontal, left, unossified area(s)Limb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete6 F 23.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short7 M 30.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete8 M 39.8 vs THORAX: AORTIC ARCH, INTERRUPTEDHead: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, completeVariants: 13th right rib, short9 M 37.6 vsLimb bones: 2nd phalanx, 1st digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedVariants: 13th bilateral rib, complete10 M 36.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 203MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1568 9vs 1 F 42.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified2 M 44.3 vsNo abnormality detected3 F 41.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified4 F 39.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSuture/fontanel: nasal suture, sutural bone5 M 29.2 vsLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified6 F 40.3 vs Head: frontal, right, unossified area(s)Variants: sternebra(e), 5th, unossified7 M 39.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, rudimentary, non-articulatedVariants: 13th right rib, short8 F 40.6 vsNo abnormality detected9 M 42.6 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified1569 8vs 1 M 48.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, completeVariants: 13th right rib, short2 M 40.4 vs Head: frontal, right, unossified area(s)Limb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete3 M 38.0 vs Head: frontal, right, unossified area(s)Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified4 M 30.7 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedHuntingdon Life Sciences09-4348Page 204MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1569 8vs 4 M 30.7 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossified5 F 29.7 vs Variants: 13th left rib, completeVariants: 13thright rib, rudimentary6 M 39.7 vs Head: frontal, left, unossified area(s)7 M 43.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short8 M 50.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, short1570 8vs 1 F 37.0 vs Girdles: pelvic girdle, right, unilateral cranial shiftHeart: atrium, bilateral, distendedVariants: 13thbilateral rib, short2 F 38.0 vsLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified3 M 42.2 vsVariants: sternebra(e), 6th, incompletely ossified4 F 40.5 vs Suture/fontanel:posterior fontanelle, enlargedVariants: sternebra(e), 6th, incompletely ossified5 M 29.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedSuture/fontanel: anterior fontanelle, enlargedSuture/fontanel: posterior fontanelle, enlargedVariants: sternebra(e), 6th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 205MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1570 8vs 6 F 35.5 vsNo abnormality detected7 F 35.3 vsLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, unossified8 M 40.5 vsNo abnormality detected1571 8vs 1 M 46.5 vsVariants: sternebra(e), 5th, incompletely ossified2 M 47.2 vs Head: hyoid cornua, right, bent inwardsSternebrae: omosternum, left, ossified3 M 44.9 vsVariants: sternebra(e), 5th, unossified4 F 40.6 vs Head: frontal, left, unossified area(s)Limb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified5 M 36.5 vsCervical vert.: odontoid process, incompletely ossifiedVariants: sternebra(e), 5th, unossified6 F 39.0 vs Head: hyoid cornua, right, bent outwardsVariants: 13th left rib, completeVariants: 13th right rib, short7 M 43.2 vs BRAIN: LATERAL VENTRICLE, BILATERAL, HYDROCEPHALYSuture/fontanel: anterior fontanelle, enlarged8 F 45.3 vsCervical vert.: odontoid process, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified1572 9vs 1 F 42.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedHuntingdon Life Sciences09-4348Page 206MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 1 - 0 ppm1572 9vs 1 F 42.8 vsVariants: sternebra(e), 6th, incompletely ossified2 M 40.6 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossified3 F 40.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete4 M 40.4 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, short5 F 32.8 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, rudimentary, non-articulated6 F 38.5 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossified7 F 40.0 vsLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short8 M 46.2 vs Variants: 13th right rib, short9 F 46.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 207MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2551 7vs 1 F 48.4 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified2 M 45.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: frontal suture, plaque of bone3 M 42.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thright rib, complete4 M 44.4 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified5 M 42.7 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified6 M 37.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified7 M 46.3 vsNo abnormality detected2552 4vs 1 F 41.8 vs Variants: 13th right rib, short, non-articulated2 M 40.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, complete3 M 48.1 vs Variants: 13th bilateral rib, complete4 M 45.2 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 208MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2553 10vs 1 M 36.2 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 5th, incompletely ossified2 M 34.2 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified3 F 34.9 vs Cervical: thymus gland, enlargedHeart: pericardium, distendedVariants: 13th left rib, shortVariants: sternebra(e), 5th, unossified4 M 38.4 vs Lungs: accessory lung lobe, absentVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified5 M 39.3 vs Variants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 5th, unossified6 F 37.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete7 F 40.2 vs Cervical: thymus gland, partially undescended, left sideVariants: sternebra(e), 5th, unossified8 M 38.2 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified9 M 39.8 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th left rib, completeHuntingdon Life Sciences09-4348Page 209MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2553 10vs 9 M 39.8 vs Variants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified10 F 41.7 vs Variants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified2554 8vs 1 M 42.0 vs Head: frontal, left, unossified area(s)2 M 26.9 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete3 M 41.4 vs Head: frontal, left, unossified area(s)Variants: 13th left rib, shortVariants: 13thright rib, complete4 F 30.1 vsNo abnormality detected5 M 36.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified6 M 39.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified7 F 38.1 vs Variants: 13th left rib, short8 F 42.3 vs Variants: 13th left rib, completeHuntingdon Life Sciences09-4348Page 210MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2554 8vs 8 F 42.3 vs Variants: 13th right rib, short2555 9vs 1 M 39.5 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th left rib, short2 M 31.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified3 M 32.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 6th, incompletely ossified4 M 30.9 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, complete5 M 30.5 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete6 F 32.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 211MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2555 9vs 7 M 29.0 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete8 F 37.2 vs Head: interparietal, supraoccipital, fusedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified9 M 41.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified2557 9vs 1 M 40.6 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedThorax: subclavian artery, branching variation, left sideVariants: sternebra(e), 6th, incompletely ossified2 M 34.4 vs Girdles: pelvic girdle, right, unilateral caudal shiftLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified3 F 27.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th leftrib, short, non-articulatedVariants: sternebra(e), 6th, unossifiedHuntingdon Life Sciences09-4348Page 212MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2557 9vs 4 F 22.5 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified5 M 30.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified6 M 34.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 6th, unossified7 F 30.1 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 6th, unossified8 M 36.4 vsLimb bones: 2nd phalanx,5th digit, bilateralforelimb, unossifiedVariants: 13thright rib, complete9 F 36.9 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete2558 5vs 1 F 44.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified2 F 41.6 vs Cervical: thymus gland, bilateral, partially undescendedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified3 F 41.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 213MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2558 5vs 3 F 41.7 vs Sternebrae: sternebral center(s), 4th-5th,bridge of ossificationSuture/fontanel: posterior fontanelle, enlargedVariants: sternebra(e), 5th, incompletely ossified4 F 44.3 vs Cervical: thymus gland, partially undescended, left sideLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified5 M 44.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedRibs: 8th, right, branchedThoracic vert.: 18 thoracolumbar vertebrae, present2559 6vs 1 F 43.6 vs Variants: 13th bilateral rib, complete2 F 41.5 vsNo abnormality detected3 F 44.8 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th left rib, shortVariants: 13thright rib, complete4 M 45.3 vs Girdles: pelvic girdle, right, unilateral caudal shiftVariants: 13th bilateral rib, complete5 F 48.2 vs Variants: 13th bilateral rib, complete6 F 44.9 vs Variants: 13th bilateral rib, complete2560 9vs 1 F 49.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13thbilateral rib, short2 M 46.7 vs Head: hyoid cornua, left, bent outwardsHuntingdon Life Sciences09-4348Page 214MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2560 9vs 2 M 46.7 vsLimb bones: 2nd phalanx, 5th digit, leftforelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLungs: accessory lung lobe, absentVariants: 13th left rib, completeVariants: 13th right rib, short3 F 43.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete4 F 42.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete5 M 38.6 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified6 F 39.2 vs Head: hyoid cornua, right, bent outwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: parietal suture, sutural boneVariants: 13th bilateral rib, complete7 M 39.9 vs Girdles: pelvic girdle, right, unilateral cranial shiftVariants: 13th bilateral rib, complete8 M 49.3 vs Head: frontal, left, unossified area(s)Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, short9 F 31.1 vs Abdomen:spleen,smallAbdomen/trunk: umbilicus, herniaLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, left,incompletely ossifiedLimb bones: talus,right, unossifiedHuntingdon Life Sciences09-4348Page 215MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2560 9vs 9 F 31.1 vsLimb bones: 1st phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 1st digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx,2nd digit, bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 2nd digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLimb bones: phalanx, 1st digit,bilateral forelimb, unossifiedLungs: accessory lung lobe, absentVariants: 13th bilateral rib, complete2561 9vs 1 M 42.1 vs Variants: 13th left rib, completeVariants: 13thright rib, short, floating2 M 41.5 vs Variants: 13th bilateral rib, complete3 F 41.1 vsHead: anterior supraorbital notch,left, incompletely ossifiedVariants: 13th bilateral rib, complete4 M 36.1 vs Thorax: subclavian artery, branching variation, left sideVariants: 13th bilateral rib, complete5 F 40.3 vs Variants: 13th left rib, completeVariants: 13th right rib, short6 F 39.6 vsHead: anterior supraorbital notch,left, incompletely ossifiedHuntingdon Life Sciences09-4348Page 216MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2561 9vs 6 F 39.6 vs Variants: 13th bilateral rib, short7 F 33.8 vsHead: anterior supraorbital notch,right, incompletely ossifiedVariants: 13th bilateral rib, complete8 F 39.2 vs Variants: 13th bilateral rib, complete9 F 47.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short2562 11vs 1 M 43.6 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, rudimentary, non-articulated2 M 33.9 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete3 F 33.7 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete4 F 35.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete5 M 33.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 217MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2562 11vs 6 M 41.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete7 M 26.5 vsGirdles: pubis, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedThorax: subclavian artery, left side, additional8 F 28.7 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short9 M 37.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThorax: subclavian artery, branching variation, left sideVariants: 13th bilateral rib, complete10 F 39.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossified11 F 43.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 218MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2563 8vs 1 F 34.8 vs Cervical: thymus gland, bilateral, partially undescendedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSternebrae: sternebral center(s), 4th, misalignedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified2 M 46.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified3 F 39.4 vs Suture/fontanel: parietal suture, sutural boneVariants: 13th left rib, rudimentaryVariants: 13th right rib, short4 M 32.1 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified5 F 24.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortHuntingdon Life Sciences09-4348Page 219MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2563 8vs 5 F 24.3 vsVariants: sternebra(e), 6th, unossified6 F 26.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13thright rib, rudimentaryVariants: sternebra(e), 6th, unossified7 M 34.4 vs Variants:13th bilateral rib, shortVariants: sternebra(e), 6th, incompletely ossified8 M 44.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 6th, unossified2564 9vs 1 F 45.3 vs Variants: 13th bilateral rib, complete2 F 48.3 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th left rib, shortVariants: sternebra(e), 6th, incompletely ossified3 M 49.5 vs Variants: 13th right rib, short4 M 42.6 vs Girdles: pelvic girdle, right, unilateral cranial shiftVariants: 13th bilateral rib, complete5 F 37.8 vsCervical vert.: odontoid process, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified6 F 38.8 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedHuntingdon Life Sciences09-4348Page 220MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2564 9vs 6 F 38.8 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified7 M 36.9 vs Lungs: accessory lung lobe, absentVariants: 13th right rib, rudimentary, non-articulated8 F 40.7 vs Ribs:13th, right, interruptedVariants: 13th left rib, completeVariants: sternebra(e), 6th, incompletely ossified9 F 42.5 vsVariants: sternebra(e), 5th, incompletely ossified2565 8vs 1 F 33.8 vs Cervical vert.:cervical rib, bilateral,without costal cartilageLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified2 F 36.4 vsHead: interparietal,incompletely ossifiedHead: interparietal, supraoccipital, bridge of ossificationLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified3 M 41.2 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th leftrib, short, non-articulated4 F 31.6 vsHead: interparietal,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 221MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2565 8vs 5 M 37.7 vsHead: interparietal,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified6 M 41.0 vs ABDOMEN/TRUNK: ABDOMEN, GASTROSCHISISCervical vert.: cervical rib, bilateral, without costal cartilageVariants: sternebra(e), 4th, unossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified7 F 39.7 vsVariants: sternebra(e), 5th, unossified8 M 42.2 vs Head: interparietal,supraoccipital, bridge of ossificationLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified2566 10vs 1 M 44.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossified2 M 44.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete3 F 41.0 vs Variants: 13th bilateral rib, complete4 M 33.0 vsLimb bones: 2nd phalanx, 5th digit,left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: 13th bilateral rib, complete5 M 32.4 vs Head: frontal, right, unossified area(s)Huntingdon Life Sciences09-4348Page 222MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2566 10vs 5 M 32.4 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 6th, unossified6 M 29.7 vs Variants: 13th left rib, short7 M 36.6 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete8 M 42.7 vs Variants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified9 F 42.1 vsLimb bones: 2nd phalanx, 5th digit, bilateralforelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified10 M 43.7 vs Variants:13th bilateral rib, short2567 10vs 1 F 50.0 vs Suture/fontanel: anterior fontanelle, enlargedVariants: sternebra(e), 5th, incompletely ossified2 F 40.3 vsVariants: sternebra(e), 5th, unossified3 M 41.3 vsVariants: sternebra(e), 6th, incompletely ossified4 F 34.1 vsVariants: sternebra(e), 5th, unossified5 F 30.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSternebrae: sternebral center(s), 6th, bipartite ossified6 F 31.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedHuntingdon Life Sciences09-4348Page 223MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2567 10vs 6 F 31.3 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th right rib, short7 M 39.9 vs Head: frontal, left, unossified area(s)8 F 44.2 vsNo abnormality detected9 M 39.6 vsVariants: sternebra(e), 5th, incompletely ossified10 F 32.1 vs Head: frontal, bilateral, suture within, partially fusedHead: hyoid cornua, right, bent inwardsHead: hyoid cornua, left, unossifiedSternebrae: sternebral center(s),1st, cranial to, supernumerary ossificationSternebrae: sternebral center(s), 2nd, bipartite ossifiedSuture/fontanel: frontal/parietal suture, bilateral, irregularSuture/fontanel: nasal suture, sutural boneSuture/fontanel: parietal suture, sutural boneVariants: 13th left rib, shortVariants: sternebra(e), 6th, incompletely ossified2568 8vs 1 M 53.7 vs Head: frontal, right, unossified area(s)Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified2 M 48.4 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thbilateral rib, shortHuntingdon Life Sciences09-4348Page 224MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2568 8vs 3 M 48.0 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSuture/fontanel: frontal suture, sutural boneSuture/fontanel: parietal suture, sutural boneVariants: 13th left rib, shortVariants: 13th right rib, short, non-articulated4 F 46.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified5 M 40.0 vsLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified6 F 43.2 vs Head: hyoid cornua, left, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel:parietal suture, irregular7 F 47.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSuture/fontanel:parietal suture, irregularVariants: sternebra(e), 5th, unossified8 F 50.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified2569 10vs 1 F 35.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedSuture/fontanel: anterior fontanelle, enlarged2 F 34.4 vs Cervical: thymus gland, partially undescended, right sideLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedHuntingdon Life Sciences09-4348Page 225MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2569 10vs 2 F 34.4 vs Sternebrae: sternebralcenter(s), 1st, cranial to, supernumerary ossificationSuture/fontanel: anterior fontanelle, enlargedVariants: 13th right rib, short3 F 34.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedLungs: accessory lung lobe, absent4 F 30.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, completeVariants: 13th right rib, short5 M 29.4 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedThorax: subclavian artery, branching variation, left side6 F 27.2 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1stdigit, left, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified7 F 28.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 2nd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 226MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2569 10vs 7 F 28.6 vsLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossifiedLungs: accessory lung lobe, absent8 F 31.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th leftrib, short, non-articulated9 F 35.6 vsLimb bones: 2nd phalanx, 2nd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: phalanx, 1st digit, bilateral forelimb, incompletely ossified10 M 36.0 vsLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified2570 9vs 1 F 40.1 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 227MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2570 9vs 1 F 40.1 vs Liver: gallbladder, rudimentaryVariants: sternebra(e), 5th, unossified2 F 42.0 vs Head: frontal,bilateral, unossified area(s)Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified3 F 38.1 vs Head: hyoid cornua, left, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified4 M 30.7 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 2nd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 3rd digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLimb bones: phalanx, 1st digit,bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified5 M 38.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified6 F 36.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedHuntingdon Life Sciences09-4348Page 228MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2570 9vs 6 F 36.1 vsVariants: sternebra(e), 5th, unossified7 M 32.5 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1stdigit, left, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, shortVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified8 F 35.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified9 M 37.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified2571 10vs 1 F 47.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified2 F 39.7 vsLimb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified3 F 48.8 vsHead: anterior supraorbital notch,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 229MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2571 10vs 4 M 37.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified5 M 37.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThorax: subclavian artery, left side, additional6 M 37.9 vs GIRDLES: CLAVICLE, LEFT, BENTLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified7 F 37.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified8 F 42.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified9 M 43.9 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified10 F 38.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified2572 10vs 1 M 39.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified2 F 38.2 vsVariants: sternebra(e), 5th, unossified3 F 38.1 vs Cervical: thymus gland, partially undescended, left sideLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13thbilateral rib, short4 M 33.8 vsHead: premaxilla, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossified5 F 34.3 vsLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: frontal suture, plaque of boneHuntingdon Life Sciences09-4348Page 230MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 2 - 2500 ppm2572 10vs 5 F 34.3 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13thbilateral rib, short6 F 30.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossified7 M 33.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossified8 M 36.9 vs Cervical: thymus gland, partially undescended, right sideLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete9 F 43.8 vs Lungs: accessory lung lobe, absent10 F 42.9 vs Lungs: accessory lung lobe, absentVariants: 13th leftrib, short, non-articulatedVariants: sternebra(e), 5th, unossifiedHuntingdon Life Sciences09-4348Page 231MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3551 6vs 1 F 45.4 vs Variants: 13th bilateral rib, complete2 F 41.0 vs Variants: 13th left rib, completeVariants: sternebra(e), 5th, incompletely ossified3 M 45.5 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified4 F 38.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: posterior fontanelle, enlargedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossified5 F 37.3 vs Head: hyoid cornua, right, bent inwardsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified6 F 44.6 vsVariants: sternebra(e), 5th, unossified3552 12vs 1 M 42.1 vs Variants: 13th bilateral rib, short2 F 38.4 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thbilateral rib, short3 M 37.0 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossified4 F 36.3 vsNo abnormality detectedHuntingdon Life Sciences09-4348Page 232MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3552 12vs 5 M 32.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossified6 M 25.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified7 M 34.8 vs Variants: 13th right rib, short8 F 32.0 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1stdigit, left, unossifiedThorax: subclavian artery, left side, additionalVariants: sternebra(e), 5th, incompletely ossified9 M 23.3 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossified10 M 28.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedThorax: subclavian artery, left side, additional11 M 39.1 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete12 M 48.7 vsNo abnormality detected3553 9vs 1 M 39.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 233MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3553 9vs 2 M 37.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified3 M 35.8 vsHead: anterior supraorbital notch,left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, short, non-articulatedVariants: sternebra(e), 5th, incompletely ossified4 F 35.6 vsHead: anterior supraorbital notch, left, incompletely ossifiedVariants: 13th bilateral rib, complete5 F 34.9 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossified6 M 32.5 vsHead: anterior supraorbital notch,right, incompletely ossifiedHead: frontal,right, unossified area(s)Limb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossified7 M 37.2 vsHead: anterior supraorbital notch,right, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete8 M 37.0 vsHead: anterior supraorbital notch,left, incompletely ossifiedLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified9 F 36.7 vs Head: hyoid cornua, bilateral, bent inwardsHuntingdon Life Sciences09-4348Page 234MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3553 9vs 9 F 36.7 vsHead: jugal, right, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th leftrib, short, non-articulated3554 9vs 1 M 31.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: sternebra(e), 1st, incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified2 F 32.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedThorax: commoncarotid artery, branchingvariation, left sideVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified3 F 35.7 vs Variants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified4 F 27.5 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1stdigit, left, unossifiedLiver: gallbladder, rudimentaryVariants: sternebra(e), 5th, unossified5 M 13.9 vs Cervical: thymus gland, smallGirdles: pubis, bilateral, unossifiedHuntingdon Life Sciences09-4348Page 235MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3554 9vs 5 M 13.9 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedSuture/fontanel: posterior fontanelle, enlargedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified6 F 26.4 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified7 F 31.8 vsLimb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete8 M 34.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th bilateral rib, complete9 M 33.2 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedHuntingdon Life Sciences09-4348Page 236MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3555 8vs 1 F 43.9 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified2 F 33.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete3 F 39.8 vs GIRDLES: CLAVICLE, LEFT, BENTLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedThoracic vert.: thoracic vert. centrum(a), 2nd, bipartite ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified4 F 36.0 vsNo abnormality detected5 F 33.3 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: 13th right rib, short6 F 38.8 vs GIRDLES: CLAVICLE, RIGHT, BENTVariants: 13th left rib, completeVariants: 13th right rib, short7 M 41.5 vs Sternebrae: sternebral center(s), 4th-5th, bridge of ossificationVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified8 F 39.0 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSternebrae: sternebral center(s), 4th-5th, bridge of ossificationVariants: 13th bilateral rib, complete3556 9vs 1 F 48.3 vs Variants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 237MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3556 9vs 2 F 43.4 vs Thorax: subclavian artery, left side, additionalVariants: 13th bilateral rib, complete3 F 44.2 vs Variants: 13th bilateral rib, complete4 F 45.3 vs Variants: 13th bilateral rib, complete5 F 45.0 vs Variants: 13th bilateral rib, complete6 F 38.3 vs Variants: 13th left rib, completeVariants: 13th right rib, short7 F 44.5 vs Variants: 13th bilateral rib, complete8 M 46.5 vs Variants:13th bilateral rib, short9 F 46.7 vs Variants: 13th bilateral rib, complete3557 10vs 1 F 44.8 vsHead: anterior supraorbital notch, left, incompletely ossifiedHead: frontal, left, unossified area(s)Limb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thright rib, completeVariants: sternebra(e), 6th, unossified2 M 41.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel:parietal suture, irregularVariants: 13th left rib, completeVariants: sternebra(e), 6th, unossified3 F 36.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 6th, unossifiedHuntingdon Life Sciences09-4348Page 238MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3557 10vs 4 F 34.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete5 M 36.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, short, non-articulated6 M 36.4 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 6th, incompletely ossified7 M 40.7 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, rudimentary, non-articulated8 F 43.6 vs Cervical: thymus gland, partially undescended, left sideLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLungs: left lung lobe, cystVariants: sternebra(e), 6th, unossified9 M 42.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 6th, incompletely ossified10 F 43.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified3558 7vs 1 M 52.3 vs Cervical: thymus gland, partially undescended, left sideHuntingdon Life Sciences09-4348Page 239MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3558 7vs 1 M 52.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thbilateral rib, short2 M 45.0 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified3 F 54.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete4 M 35.6 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedSternebrae: sternebral center(s),5th-6th, supernumerary ossification, betweenVariants: 13th bilateral rib, complete5 F 41.4 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified6 F 46.7 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified7 M 46.9 vsCervical vert.: odontoid process, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: parietal suture, sutural boneVariants: 13th bilateral rib, complete3559 6vs 1 M 52.1 vsNo abnormality detected2 F 40.6 vs HEART: VENTRICULAR SEPTUM, SMALL, MEMBRANOUS, VENTRICULAR SEPTAL DEFECTCervical vert.: cervical hemicentrum(a), 4th, left, unossifiedEye: eye, bilateral, smallHuntingdon Life Sciences09-4348Page 240MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3559 6vs 2 F 40.6 vsHead: interparietal,incompletely ossifiedHeart: heart, malrotatedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedLungs: accessory lung lobe, absentLungs: lungs (general), smallSternebrae: sternebral center(s), 2nd-3rd,supernumerary ossification, partially fused, betweenSternebrae: sternebral center(s), 3rd-4th, bridge of ossificationThoracic vert.: 20 thoracolumbar vertebrae, presentThorax: caudal vena cava, distended, malpositionedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified3 M 40.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: parietal suture, sutural boneThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete4 F 39.7 vsCervical vert.: odontoid process, ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedHuntingdon Life Sciences09-4348Page 241MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3559 6vs 4 F 39.7 vs Liver: gallbladder, rudimentaryLungs: accessory lung lobe, absentSuture/fontanel: parietal suture, sutural boneThoracic vert.: 20 thoracolumbar vertebrae, presentThoracic vert.: thoracic vert. centrum(a), 8th, incompletely ossifiedVariants: 13th bilateral rib, complete5 F 48.3 vsHead: premaxilla, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete6 M 47.6 vsHead: premaxilla, bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLungs: accessory lung lobe, smallThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete3560 8vs 1 M 36.4 vsLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified2 F 32.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, complete3 F 35.8 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 242MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3560 8vs 3 F 35.8 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete4 M 30.2 vs Heart: heart, smallLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateralforelimb, unossifiedVariants: 13thright rib, complete5 M 35.7 vs Head: frontal, right, unossified area(s)Limb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th left rib, short6 M 33.5 vsGirdles: pubis, bilateral, incompletely ossifiedHeart: atrium, bilateral, enlargedHeart: heart, smallHeart: ventricle, right, smallLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified7 F 33.2 vs Heart: heart, smallLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedHuntingdon Life Sciences09-4348Page 243MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3560 8vs 7 F 33.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified8 F 39.9 vs Head: frontal, left, unossified area(s)Heart: atrium, right, irregularLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 4th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, right forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLiver: gallbladder, rudimentary3561 7vs 1 F 45.8 vsHead: anterior supraorbital notch,right, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified2 M 43.1 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete3 M 39.4 vs Girdles: clavicle, left, thickenedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified4 M 39.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified5 F 40.1 vs Variants: 13th bilateral rib, complete6 F 42.6 vs Variants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossifiedHuntingdon Life Sciences09-4348Page 244MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3561 7vs 7 M 45.5 vs Variants: 13th bilateral rib, complete3562 7vs 1 M 35.0 vsVariants: sternebra(e), 5th, unossified2 M 36.8 vs Heart: atrium, bilateral, distended3 M 39.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified4 M 40.8 vs Heart: atrium, bilateral, distendedHeart: heart, smallLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified5 M 39.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified6 F 44.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified7 F 43.4 vsVariants: sternebra(e), 5th, unossified3563 13vs 1 M 44.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified2 F 37.6 vsLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified3 F 30.0 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateralforelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedThorax: subclavian artery, branching variation, left sideHuntingdon Life Sciences09-4348Page 245MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3563 13vs 4 F 30.4 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified5 F 33.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified6 F 26.3 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified7 M 26.3 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossifiedHuntingdon Life Sciences09-4348Page 246MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3563 13vs 8 F 35.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, right forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, righthind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified9 F 40.4 vs Head: interparietal,supraoccipital, bridge of ossificationVariants: sternebra(e), 5th, unossified10 F 41.9 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified11 F 40.0 vs Head: interparietal,supraoccipital, bridge of ossificationLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified12 M 32.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified13 F 41.2 vsLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: thoracic vert. centrum(a), 4th-5th, dumbbell ossifiedVariants: 13thright rib, rudimentaryVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossifiedHuntingdon Life Sciences09-4348Page 247MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3565 6vs 1 M 54.2 vsNo abnormality detected2 F 50.5 vsVariants: sternebra(e), 5th, incompletely ossified3 M 50.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossified4 M 49.5 vs Variants: 13th left rib, short5 F 50.5 vsVariants: sternebra(e), 5th, unossified6 F 54.3 vs Variants:13th bilateral rib, short3566 8vs 1 M 38.0 vs THORACIC VERT.: THORACIC HEMICENTRUM(A), 12TH-13TH, RIGHT, FUSEDTHORACIC VERT.: THORACIC VERTEBRAL ARCH(ES), 11TH, LEFT, SMALLTHORACIC VERT.: THORACIC VERTEBRAL ARCH(ES), 8TH-9TH, RIGHT, FUSEDHead: anterior supraorbital notch,left, incompletely ossifiedSuture/fontanel: parietal suture, sutural boneThoracic vert.: thoracic vert. centrum(a), 11th, asymmetrically ossifiedThoracic vert.: thoracicvert. centrum(a), 12th, bipartiteThoracic vert.: thoracic vert. centrum(a), 8th, asymmetrically ossifiedThoracic vert.: thoracicvert. centrum(a), 9th, misshapenVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified2 F 33.8 vs Head: parietal, right, unossified area(s)Limb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete3 M 33.1 vs Variants: 13th bilateral rib, complete4 M 34.2 vsHead: anterior supraorbital notch,right, incompletely ossifiedHuntingdon Life Sciences09-4348Page 248MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3566 8vs 4 M 34.2 vs Head:parietal, bilateral,unossified area(s)Limb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedVariants: 13th bilateral rib, complete5 M 31.4 vs Variants: 13th bilateral rib, complete6 M 30.1 vs Girdles:clavicle, bilateral, bentHead: hyoid cornua, bilateral, bent outwardsHead: parietal, bilateral, unossified area(s)Limb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, complete7 M 35.6 vsHead: anterior supraorbital notch,bilateral, incompletely ossifiedSuture/fontanel: nasal suture, sutural bone8 M 38.3 vs Heart: heart, smallLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified3567 7vs 1 M 47.7 vs Variants: 13th bilateral rib, complete2 M 44.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: sternebra(e), 5th, unossified3 F 40.8 vs Head: frontal, right, unossified area(s)Limb bones: metacarpal, 1st digit,right, incompletely ossifiedHuntingdon Life Sciences09-4348Page 249MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3567 7vs 3 F 40.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified4 M 47.6 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified5 F 48.2 vs Cervical: thymus gland, bilateral, partially undescendedVariants: 13th left rib, shortVariants: 13thright rib, rudimentary6 M 43.1 vs Cervical: thymus gland, bilateral, partially undescendedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified7 F 50.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified3569 11vs 1 F 39.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossified2 M 37.2 vs Variants: 13th left rib, shortVariants: 13thright rib, complete3 M 35.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified4 F 31.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 250MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3569 11vs 5 M 30.5 vsHead: hyoid body, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th leftrib, short, non-articulatedVariants: sternebra(e), 5th, incompletely ossified6 F 23.8 vsHead: hyoid body, unossifiedHead: hyoid cornua, left, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, rudimentaryVariants: sternebra(e), 6th, unossified7 F 32.8 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedThorax: subclavian artery, left side, additional8 M 33.4 vs Lungs: accessory lung lobe, rudimentaryVariants: 13th bilateral rib, rudimentary9 M 35.9 vsNo abnormality detected10 M 37.3 vs Girdles: clavicle, left, bentSuture/fontanel:parietal suture, irregular11 M 45.2 vs Head: frontal, right, unossified area(s)3570 9vs 1 M 33.0 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 251MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3570 9vs 1 M 33.0 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedLiver: gallbladder, rudimentaryVariants: 13th left rib, shortVariants: sternebra(e), 5th, unossified2 F 41.1 vsLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: sternebra(e), 5th, unossified3 M 41.9 vs Variants:13th bilateral rib, shortVariants: sternebra(e), 5th, unossified4 F 40.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, unossified5 F 39.5 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, shortVariants: 13thright rib, rudimentaryVariants: sternebra(e), 5th, incompletely ossified6 F 33.1 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 252MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3570 9vs 6 F 33.1 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified7 F 29.6 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified8 M 44.8 vs Variants:13th bilateral rib, shortVariants: sternebra(e), 5th, unossified9 F 38.5 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossified3571 3vs 1 M 46.6 vsLimb bones: 2nd phalanx, 5th digit, bilateral, unossifiedSternebrae: sternebral center(s),1st, cranial to, supernumerary ossification2 M 48.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedRibs: distal, 5th, right, nodulatedVariants: sternebra(e), 6th, incompletely ossified3 M 44.6 vsLimb bones: 2nd phalanx, 5th digit, bilateralforelimb, unossifiedVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 253MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3572 7vs 1 M 36.3 vsVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified2 F 40.1 vsHead: interparietal,incompletely ossifiedHead: parietal, bilateral, fissure(s)Variants: 13th bilateral rib, complete3 M 30.0 vsHead: hyoid body, unossifiedHead: interparietal,incompletely ossifiedHead: parietal, bilateral, fissure(s)Limb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThorax: subclavian artery, left side, additionalVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified4 F 34.5 vsVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified5 F 30.4 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossified6 M 24.2 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: epiphyses, unossifiedVariants: 13th left rib, rudimentary, non-articulatedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified7 M 39.1 vsHead: hyoid body, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedHuntingdon Life Sciences09-4348Page 254MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 3 - 10000 ppm3572 7vs 7 M 39.1 vs Thorax: subclavian artery, branching variation, left sideVariants: 13th bilateral rib, rudimentaryVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossifiedHuntingdon Life Sciences09-4348Page 255MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4551 11vs 1 M 47.2 vs Head: hyoid cornua, bilateral, bent inwards2 M 29.8 vsNo abnormality detected3 M 34.1 vs Lungs: accessory lung lobe, rudimentaryVariants: 13th right rib, short4 F 37.6 vsNo abnormality detected5 M 32.8 vsNo abnormality detected6 M 31.1 vs Cervical: thymus gland, partially undescended, right sideLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: frontal suture, plaque of boneVariants: 13thright rib, complete7 M 33.1 vs Cervical: thymus gland, partially undescended, right sideThorax: subclavian artery, left side, additionalVariants: 13th bilateral rib, complete8 F 38.3 vs Head: parietal, right, unossified area(s)Variants: 13th bilateral rib, complete9 M 40.9 vsVariants: sternebra(e), 5th, incompletely ossified10 M 42.9 vs Cervical: thymus gland, partially undescended, right sideVariants: 13thbilateral rib, short11 F 46.4 vs Cervical: thymus gland, bilateral, partially undescendedLungs: accessory lung lobe, smallVariants: 13th bilateral rib, complete4552 8vs 1 M 44.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, shortHuntingdon Life Sciences09-4348Page 256MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4552 8vs 1 M 44.6 vsVariants: sternebra(e), 6th, unossified2 M 43.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThorax: subclavian artery, branching variation, left side3 M 42.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short4 F 43.9 vsLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete5 M 47.1 vs Cervical: thymus gland, bilateral, partially undescendedVariants: 13th bilateral rib, completeVariants: sternebra(e), 6th, unossified6 F 41.7 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified7 F 43.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, short8 F 46.8 vs Cervical: thymus gland, bilateral, partially undescendedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 6th, incompletely ossified4553 8vs 1 M 48.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 257MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4553 8vs 2 M 35.6 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1stdigit, left, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th leftrib, short, non-articulatedVariants: 13th right rib, short3 M 36.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete4 M 43.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSuture/fontanel: frontal suture, plaque of boneVariants: 13th bilateral rib, complete5 M 45.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete6 M 48.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, short7 F 46.9 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedSternebrae: sternebral center(s),5th-6th, supernumerary ossification, betweenVariants: 13th bilateral rib, complete8 M 47.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 258MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4554 9vs 1 M 47.2 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossified2 M 43.4 vs GIRDLES: CLAVICLE, LEFT, BENTLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThorax: subclavian artery, left side, additional3 M 36.7 vs GIRDLES: CLAVICLE, RIGHT, BENTLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th left rib, rudimentary, non-articulatedVariants: 13thright rib, complete4 M 41.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete5 M 37.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short6 M 38.1 vs GIRDLES: CLAVICLE, RIGHT, BENTLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 6th, incompletely ossified7 M 42.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete8 F 41.9 vs GIRDLES: CLAVICLE, LEFT, ENLARGEDGIRDLES: SCAPULA, LEFT, ENLARGEDLIMB BONES: HUMERUS, LEFT, THICKENEDHuntingdon Life Sciences09-4348Page 259MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4554 9vs 8 F 41.9 vs LIMB BONES: METACARPAL, 2ND DIGIT, LEFT, THICKENEDLIMB BONES: RADIUS, LEFT, THICKENEDLIMB BONES: ULNA, LEFT, MISSHAPEN, THICKENEDLIMB BONES: 1ST PHALANX, 2ND DIGIT, 3RD DIGIT, LEFT, THICKENEDLUMBAR VERT.: LUMBAR TRANSVERSE PROCESS, 2ND-7TH, LEFT, ENLARGEDLUMBAR VERT.: LUMBAR TRANSVERSE PROCESS, 5TH-7TH, RIGHT, ENLARGEDHead: parietal, left, unossified area(s)Paws/limbs: forelimb(s),left, lower, upper, thickenedPaws/limbs: forepaw(s),left, digit(s), second, misshapenPaws/limbs: left forepaw, digit(s), third, misshapenWhole body: lumbar spine, musculature, enlarged9 M 49.4 vs Variants: 13th left rib, rudimentary4555 9vs 1 F 38.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short2 M 39.0 vs Cervical: thymus gland, bilateral, partially undescendedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedSternebrae: sternebral center(s),1st, cranial to, supernumerary ossificationVariants: sternebra(e), 5th, unossified3 M 39.2 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, shortHuntingdon Life Sciences09-4348Page 260MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4555 9vs 3 M 39.2 vs Variants: 13th right rib, complete4 F 37.7 vs Variants:13th right rib, complete5 F 30.9 vs Head: parietal, right, fissure(s)Limb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossified6 M 35.4 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedVariants: 13thbilateral rib, short7 F 29.0 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 6th, incompletely ossified8 M 33.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedLiver: gallbladder, rudimentaryVariants: 13thbilateral rib, short9 F 44.3 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossified4556 6vs 1 F 43.5 vs Variants: 13th left rib, completeVariants: 13thright rib, rudimentary2 M 47.3 vs Girdles: pelvic girdle, right, unilateral caudal shiftVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 261MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4556 6vs 3 F 42.9 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th left rib, completeVariants: 13th right rib, short4 F 45.1 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete5 M 46.1 vs Thoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete6 M 45.2 vs Variants: 13th right rib, short4557 9vs 1 M 36.1 vsLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: 13thbilateral rib, short2 M 35.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified3 M 35.0 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short4 F 37.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete5 F 34.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedThorax: subclavian artery, branching variation, left sideVariants: 13th bilateral rib, completeHuntingdon Life Sciences09-4348Page 262MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4557 9vs 5 F 34.5 vsVariants: sternebra(e), 6th, unossified6 F 34.5 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThorax: subclavian artery, branching variation, left side7 M 34.4 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified8 M 38.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossified9 M 39.7 vsHead: anterior supraorbital notch,right, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossified4558 9vs 1 M 47.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, short2 F 42.0 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, short3 F 38.8 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified4 F 35.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossifiedHuntingdon Life Sciences09-4348Page 263MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4558 9vs 5 F 30.4 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th right rib, short, non-articulated6 F 37.5 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossified7 M 45.8 vs Variants: 13th bilateral rib, complete8 F 39.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th right rib, short9 M 49.8 vsLimb bones: metacarpal, 1st digit,right, incompletely ossified4559 5vs 1 F 50.5 vsHead: premaxilla, left,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, short2 F 50.0 vs Suture/fontanel: frontal suture, plaque of bone3 M 54.2 vsNo abnormality detected4 M 53.4 vsVariants: sternebra(e), 5th, unossified5 M 56.2 vs Head: hyoid cornua, left, bent outwardsVariants: sternebra(e), 5th, unossified4560 9vs 1 F 43.5 vsLimb bones: metacarpal, 1stdigit, left, incompletely ossifiedVariants: sternebra(e), 5th, unossified2 F 46.7 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedHuntingdon Life Sciences09-4348Page 264MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4560 9vs 2 F 46.7 vs Variants: 13th left rib, shortVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified3 M 48.0 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th left rib, shortVariants: 13thright rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified4 F 43.7 vsHead: anterior supraorbital notch,left, incompletely ossifiedHead: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified5 M 44.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified6 F 37.0 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13thright rib, completeVariants: sternebra(e), 5th, unossified7 F 37.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: sternebra(e), 5th, unossified8 M 42.8 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedHuntingdon Life Sciences09-4348Page 265MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4560 9vs 8 M 42.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified9 F 45.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified4561 6vs 1 F 50.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete2 F 45.5 vs Variants: 13th bilateral rib, complete3 M 45.6 vs Variants: 13th bilateral rib, complete4 M 39.8 vs Variants: 13th left rib, completeVariants: 13th right rib, short5 M 49.2 vs Variants: 13th bilateral rib, complete6 M 50.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete4562 9vs 1 M 42.6 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSuture/fontanel: frontal suture, plaque of boneVariants: 13th left rib, complete2 M 40.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossifiedHuntingdon Life Sciences09-4348Page 266MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4562 9vs 2 M 40.2 vsVariants: sternebra(e), 6th, incompletely ossified3 M 38.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, rudimentary, non-articulated4 F 33.9 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedThorax: subclavian artery, branching variation, left sideVariants: 13th bilateral rib, complete5 F 33.4 vsHead: hyoid body, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short6 F 33.3 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossified7 F 35.0 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete8 M 36.4 vs Head: interparietal,supraoccipital, bridge of ossificationLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified9 M 41.2 vs Variants: 13th left rib, completeVariants: 13th right rib, short, non-articulated4563 12vs 1 M 43.9 vs Cervical vert.:cervical rib, right, complete with costal cart.Cervical vert.: cervicalrib, left, without costal cartilageHead: hyoid cornua, left, bent inwardsLimb bones: 2nd phalanx, 5th digit, left forelimb, unossifiedHuntingdon Life Sciences09-4348Page 267MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4563 12vs 1 M 43.9 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified2 F 38.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, incompletely ossified3 M 42.0 vs Head: hyoid cornua, left, bent inwardsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, complete4 F 39.3 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, righthind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, unossified5 F 35.0 vs HEART: PAPILLARY MUSCLE, RUDIMENTARYAbdomen: abdominal cavity, fluid filledCervical vert.: odontoid process, incompletely ossifiedHead: hyoid body, unossifiedHeart: bilateral ventricles, enlargedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus,left, unossifiedLimb bones: talus, right,incompletely ossifiedLimb bones: 2nd phalanx, 1st digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx,2nd digit, bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 268MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4563 12vs 5 F 35.0 vsLimb bones: 2nd phalanx,2nd digit, bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 3rd digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx,3rd digit, bilateralhind limb, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral forelimb, unossifiedLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLimb bones: phalanx, 1st digit,right, incompletely ossifiedLimb bones: phalanx, 1st digit, left, unossifiedLiver: all lobes, enlargedLungs: lungs (general), rudimentaryThorax: aorta, distendedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossifiedWhole body: skin/subcutis, edema6 F 33.3 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: frontal suture, plaque of boneVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossifiedHuntingdon Life Sciences09-4348Page 269MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4563 12vs 6 F 33.3 vsVariants: sternebra(e), 6th, unossified7 F 31.7 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossified8 M 28.6 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses, unossifiedVariants: 13thbilateral rib, shortVariants: sternebra(e), 5th, unossified9 M 35.1 vsHead: hyoid body, unossifiedHead: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified10 F 36.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, rudimentary, non-articulatedVariants: 13thright rib, completeVariants: sternebra(e), 5th, unossified11 M 44.8 vsNo abnormality detected12 M 43.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, completeVariants: sternebra(e), 5th, unossifiedHuntingdon Life Sciences09-4348Page 270MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4563 12vs 12 M 43.9 vsVariants: sternebra(e), 6th, unossified4564 10vs 1 M 47.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: parietal suture, plaque of boneThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete2 F 41.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete3 F 39.9 vs Head: hyoid cornua, bilateral, bent inwardsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th left rib, completeVariants: 13th right rib, short, non-articulatedVariants: sternebra(e), 6th, unossified4 M 38.2 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th bilateral rib, complete5 M 28.9 vsLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short, non-articulated6 F 30.6 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedHuntingdon Life Sciences09-4348Page 271MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4564 10vs 6 F 30.6 vsLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete7 M 33.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedLimb bones: epiphyses,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete8 M 37.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, complete9 M 39.1 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th leftrib, short, non-articulatedVariants: 13thright rib, complete10 M 40.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete4565 11vs 1 F 44.0 vsLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: 13th bilateral rib, complete2 F 34.7 vs Variants: 13th bilateral rib, complete3 F 32.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, rudimentary, non-articulated4 F 26.2 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedHuntingdon Life Sciences09-4348Page 272MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4565 11vs 4 F 26.2 vs Variants: 13th bilateral rib, complete5 F 37.7 vs Head: nasal,bilateral, suture withinLimb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete6 M 39.1 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, rudimentary, non-articulatedVariants: 13thright rib, complete7 M 33.5 vsNo abnormality detected8 F 34.1 vs Variants: 13th bilateral rib, complete9 F 39.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th bilateral rib, complete10 F 46.6 vs Variants: 13th bilateral rib, complete11 F 26.7 vs CAUDAL VERT.: CAUDAL VERTEBRA(E), 14TH-18TH, ABSENTCAUDAL VERT.: CAUDAL VERTEBRA(E), 7TH-11TH, FUSEDLUMBAR VERT.: LUMBAR VERTEBRAL CENTRUM(A), 5TH-6TH, FUSEDAbdomen/trunk: tail, rudimentaryCaudal vert.: caudal vertebra centrum(a), 11th, bipartite ossifiedCaudal vert.: caudal vertebra centrum(a), 1st, bipartite ossifiedCaudal vert.: caudal vertebra(e), 12th-13th, asymmetrically ossifiedLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedHuntingdon Life Sciences09-4348Page 273MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4565 11vs 11 F 26.7 vsLimb bones: 2nd phalanx, 4th digit,bilateral hind limb, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedLiver: gallbladder, absentLungs: lungs (general), smallSternebrae: sternebral center(s), 4th, misalignedSternebrae: sternebral center(s), 4th-5th, bridge of ossificationSuture/fontanel: nasal suture, sutural boneVariants: 13th left rib, completeVariants: sternebra(e), 2nd, incompletely ossified4566 10vs 1 M 41.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedPaws/limbs: forelimb(s), bilateral, flexure(s)Paws/limbs: forepaw(s), bilateral, flexure(s)Paws/limbs: hindlimb(s), bilateral, flexure(s)Sternebrae: sternebralcenter(s), 2nd-5th, misalignedSternebrae: sternebral center(s), 3rd-4th, bridge of ossificationThorax: subclavian artery, left side, additional2 M 47.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedPaws/limbs: forelimb(s), bilateral, flexure(s)Paws/limbs: forepaw(s), bilateral, flexure(s)Paws/limbs: hindlimb(s), bilateral, flexure(s)Thorax: thoracic cavity, fluid filledVariants: 13th left rib, completeHuntingdon Life Sciences09-4348Page 274MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4566 10vs 2 M 47.9 vs Variants: 13th right rib, short3 F 42.0 vs Head: frontal, right, fissure(s)Variants: 13th bilateral rib, complete4 F 43.0 vs Thorax: subclavian artery, branching variation, left side5 M 33.3 vs Variants: 13th right rib, short6 F 27.2 vs Paws/limbs: forelimb(s), bilateral, flexure(s)Paws/limbs: hindlimb(s), bilateral, flexure(s)Variants: 13th bilateral rib, complete7 M 40.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedPaws/limbs: forelimb(s), bilateral, flexure(s)Paws/limbs: forepaw(s), bilateral, flexure(s)Paws/limbs: hindlimb(s), bilateral, flexure(s)Suture/fontanel:parietal suture, enlargedVariants: 13th right rib, short8 F 33.7 vs Paws/limbs: hindlimb(s), bilateral, flexure(s)Variants: 13th left rib, completeVariants: 13thright rib, rudimentary9 M 37.9 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossified10 M 42.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13thbilateral rib, shortHuntingdon Life Sciences09-4348Page 275MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4567 10vs 1 F 37.9 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossified2 F 36.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentVariants: 13th left rib, completeVariants: sternebra(e), 5th, unossified3 F 44.8 vs Variants: 13th right rib, short4 F 42.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short5 M 38.9 vs Variants:13th right rib, completeVariants: sternebra(e), 5th, incompletely ossified6 F 43.8 vsNo abnormality detected7 F 37.1 vs Variants:13th bilateral rib, shortVariants: sternebra(e), 5th, incompletely ossified8 F 28.4 vs Suture/fontanel:anterior fontanelle, enlargedVariants: 13th left rib, completeVariants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossified9 F 38.5 vsLimb bones: epiphyses,incompletely ossifiedHuntingdon Life Sciences09-4348Page 276MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4567 10vs 10 F 45.3 vsVariants: sternebra(e), 5th, incompletely ossified4568 11vs 1 M 49.9 vs Abdomen: jejunum, cystCervical: thymus gland, partially undescended, right sideLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedSternebrae: sternebral center(s), 3rd-4th, bridge of ossificationSternebrae: sternebral center(s), 4th, misalignedSternebrae: sternebral center(s), 4th-5th, fusedSuture/fontanel: anterior fontanelle, enlargedThorax: subclavian artery, left side, additionalVariants: 13th left rib, shortVariants: 13thright rib, complete2 F 47.4 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th right rib, shortVariants: sternebra(e), 5th, incompletely ossified3 M 40.7 vs Heart: ventricle, av-valve, right, noduleLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: 13th left rib, complete4 F 32.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th left rib, shortVariants: 13thright rib, complete5 F 33.9 vs Head: frontal, left, unossified area(s)Limb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedHuntingdon Life Sciences09-4348Page 277MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4568 11vs 5 F 33.9 vs Variants: 13th bilateral rib, short6 F 30.0 vsLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossified7 M 34.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short8 F 37.7 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th right rib, short9 M 44.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedThorax: subclavian artery, branching variation, left sideVariants: 13th leftrib, short, non-articulatedVariants: 13thright rib, complete10 M 44.4 vsVariants: sternebra(e), 5th, unossified11 F 42.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete4569 pd 10vs 1 F 42.5 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedThorax: subclavian artery, left side, additionalVariants: 13th left rib, short2 M 33.3 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1st digit, left, unossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, shortpd = premature delivery, excluded from summaryHuntingdon Life Sciences09-4348Page 278MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4569 pd 10vs 2 M 33.3 vs Variants: 13th right rib, completeVariants: sternebra(e), 6th, incompletely ossified3 F 21.6 vsHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 1st digit, left forelimb,incompletely ossifiedLimb bones: epiphyses, unossifiedSuture/fontanel: anterior fontanelle, enlargedThorax: subclavian artery, left side, additionalVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified4 F 35.0 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: 2nd phalanx, 5th digit, bilateralforelimb, unossifiedLimb bones: epiphyses, unossifiedLungs: accessory lung lobe, absentVariants: 13th left rib, rudimentary5 F 39.0 vs Head: frontal, right, unossified area(s)Limb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified6 F 24.8 vsLimb bones: metacarpal, 1st digit,right, incompletely ossifiedLimb bones: metacarpal, 1stdigit, left, unossifiedLimb bones: talus,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedpd = premature delivery, excluded from summaryHuntingdon Life Sciences09-4348Page 279MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4569 pd 10vs 6 F 24.8 vsLimb bones: epiphyses, unossifiedVariants: 13th left rib, shortVariants: sternebra(e), 5th, incompletely ossified7 F 30.4 vsLimb bones: epiphyses,incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified8 F 21.3 vsCervical vert.: odontoid process, incompletely ossifiedHead: hyoid body, unossifiedLimb bones: metacarpal, 1st digit, bilateral, unossifiedLimb bones: talus,bilateral, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 4th digit, bilateral hind limb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified9 M 29.8 vsCervical vert.: odontoid process, incompletely ossifiedLimb bones: metacarpal, 1st digit, right, unossifiedLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: epiphyses, unossifiedVariants: sternebra(e), 6th, unossified10 M 29.1 vs Head: interparietal,supraoccipital, bridge of ossificationLimb bones: metacarpal, 1st digit, left, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 6th, unossifiedpd = premature delivery, excluded from summaryHuntingdon Life Sciences09-4348Page 280MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4570 6vs 1 M 51.8 vsVariants: sternebra(e), 5th, unossified2 F 42.8 vs Girdles: clavicle, left, bentLimb bones: 2nd phalanx, 5th digit, left forelimb, incompletely ossifiedLimb bones: 2nd phalanx, 5th digit, right forelimb, unossifiedVariants: 13th left rib, shortVariants: 13th right rib, rudimentary, non-articulatedVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, unossified3 M 45.7 vsVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified4 F 39.0 vs Girdles: clavicle, right, bentLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: sternebra(e), 5th, unossified5 F 45.8 vsLimb bones: 2nd phalanx, 5th digit, bilateral forelimb, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified6 F 41.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified4571 8vs 1 F 47.5 vs Thorax: subclavian artery, left side, additionalVariants: 13thbilateral rib, short2 M 45.8 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedThorax: subclavian artery, left side, additionalHuntingdon Life Sciences09-4348Page 281MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4571 8vs 3 F 42.7 vsLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short4 F 40.6 vs Lungs: accessory lung lobe, absentThorax: subclavian artery, left side, additionalVariants: 13th left rib, completeVariants: 13th right rib, short5 M 37.7 vs Cervical: thymus gland, partially undescended, left sideLimb bones: metacarpal, 1st digit, bilateral,incompletely ossifiedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedVariants: 13th left rib, completeVariants: 13th right rib, short6 F 40.5 vs Thorax: subclavian artery, left side, additional7 M 43.3 vs Variants: 13th left rib, short8 F 44.4 vsNo abnormality detected4572 10vs 1 M 45.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: frontal suture, plaque of boneSuture/fontanel: parietal suture, plaque of boneVariants: 13th left rib, shortVariants: 13thright rib, complete2 F 41.9 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: frontal suture, plaque of boneHuntingdon Life Sciences09-4348Page 282MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4572 10vs 2 F 41.9 vs Variants: 13th bilateral rib, complete3 M 36.3 vs Head: interparietal, supraoccipital, fusedLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedSuture/fontanel: frontal suture, plaque of boneVariants: 13th leftrib, short, non-articulatedVariants: 13th right rib, shortVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified4 F 35.6 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossifiedVariants: sternebra(e), 6th, incompletely ossified5 F 32.3 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, unossifiedVariants: sternebra(e), 6th, incompletely ossified6 M 32.1 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedLimb bones: epiphyses,incompletely ossifiedSuture/fontanel: frontal suture, plaque of boneVariants: 13th left rib, shortVariants: 13thright rib, complete7 F 34.3 vs GIRDLES: CLAVICLE, BILATERAL, BENTLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedThoracic vert.: 20 thoracolumbar vertebrae, presentHuntingdon Life Sciences09-4348Page 283MA-252E-93-23FinalReportIndividual Fetal ObservationsAppendix LAnimal Weight Examination FindingsNumber Exams Fetus Sex (g) Fate Major in CAPITALS, external underlined, ossification initalicsGroup 4 - 15000 ppm4572 10vs 7 F 34.3 vs Variants: 13th bilateral rib, completeVariants: sternebra(e), 6th, incompletely ossified8 M 36.9 vs Head: hyoid cornua, right, bent inwardsHead: interparietal, supraoccipital, fusedLimb bones: 2nd phalanx, 5th digit, right forelimb, incompletely ossifiedVariants: 13th bilateral rib, completeVariants: sternebra(e), 5th, incompletely ossified9 M 40.2 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, short, non-articulated10 M 43.5 vsLimb bones: 2nd phalanx, 5th digit,bilateral forelimb, unossifiedVariants: 13th bilateral rib, complete

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Equipment List Appendix M

Exposure Chamber

1500 Liter glass and stainless steel chamber (Unifab)

Compound Generator

Counter-Current Volatilization Unit, with a coile d glass rod insert (Crown Glass Co., Inc.)

heated by a nichrome wire (Crown Gla ss Co., Inc.) monitored with a Big Digit

Humidity/Temperature Meter (VWR)

Compound Reservoir

Sponsor Cylinder

Variable Auto Transformer

Variable Autotransformer, Type 3PN 1010 (Staco Energy Products Company)

Flowmeters

Liquid flowmeter, size 0-65 mm, Tube No. 6G03-R3, 6G04-R3 (Key Instruments)

Flowmeter, size 0 – 4, 0 – 5, 0 – 10, 0 – 20, 0 - 30 or 0 - 40 Lpm (Dwyer ® 

# )

Pressure/Vacuum Gauge(s)

Norgreen backpressure gauge, P/N R07-219-RGKA

U.S. Gauge backpressure gauge

Regulator(s)

Norgreen, P/N R07-219-RGKA

Valves

Metering Valve, Model SS-4L Series (Nupro ® 

# Co.)

Toggle Valve

Tubing

Size 1/4”, 1/2”, 3/16”, PVC type, plastic (Masterkleer)

Teflon ® 

# , size 1/8”, 1/4”

1/2” stainless steel

T-Tube, stainless steel

Filters

Balston ® 

# Microfibre  Disposable Filter Units (Grade DQ)

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Equipment List Appendix M

Vacuum Pumps

Thomas Industries Inc., Model 707CM50

Balance

Pelouze 4040

Air Analyzer

MIRAN ® 

# 1A-CVF Ambient Air Analyzer (Wilks) with a Cole Parmer strip chart

recorder, Model 201, and a Micronta ® 

# LCD Benchtop Digital Multimeter No. 22-195

Particle Sizer/Analyzer

TSI Aerodynamic Particle Sizer, Model 331001, with a DELL computer, Model 486P/25,

equipped with an Epson LQ-570+ Dot matrix printer, Model P630B

Environmental Monitoring

Springfield ® 

# Apollo Temperature and Humidity Gauge, calibrated with a Digital

Hygrometer and Thermometer, Model 11-661-18 (Fisher Scientific)

Chamber Air-flow Gauge(s)

Dwyer ® 

# Magnahelic gauge, calibrated prestudy with Dry Gas Meter, Model 2M (Singer)

Chamber Static Pressure

Dwyer ® 

# Magnehelic ® 

# gauge, calibrated prestudy with Dwyer  

# Mark II Manometer,

Model 25 (Dwyer  

# Instruments Inc.)

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Pre-Study Chamber Distribution Samples Appendix N

Group

(target ppm) Date Port Concentration

(ppm) Ratio to H-1

2 (2500) 10 Mar 10 H-1 2500 -

H-2 2200 0.88

H-3 2300 0.92

H-4 2400 0.96

H-7 2000 0.80

H-8 2100 0.84

H-1 2400 -

H-9 1600 0.67

H-9 1500 0.63

H-10 1800 0.75

H-1 2700 -

H-12 3000 1.11

H-11 2700 1.00

H-14 2300 0.85

H-13 2000 0.74

11 Mar 10 H-1 2400 -

H-7 2000 0.83

H-8 2200 0.92

H-9 1900 0.79

H-1 2300 -

H-10 2400 1.04

3 (10000) 10 Mar 10 H-1 10000 -

H-2 9300 0.93

H-3 9600 0.96

H-4 9200 0.92

H-1 10000 -

H-7 9300 0.93

H-8 9300 0.93

H-9 9700 0.97

H-10 10000 1.00

H-1 10000 -

H-14 10000 1.00

H-13 10000 1.00

H-12 10000 1.00

H-11 9800 0.98

# Huntingdon Life Sciences 09-4348 Page 287

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Pre-Study Chamber Distribution Samples Appendix N

Group

(target ppm) Date Port Concentration

(ppm) Ratio to H-1

4 (15000) 10 Mar 10 H-1 14000 -

H-2 14000 1.00

H-3 14000 1.00

H-4 14000 1.00

H-7 14000 1.00

H-1 14000 -

H-8 13000 0.93

H-9 14000 1.00

H-10 14000 1.00

H-12 14000 1.00

H-1 14000 -

H-11 14000 1.00

H-14 14000 1.00

H-13 14000 1.00

# Huntingdon Life Sciences 09-4348 Page 288

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Miran ® 

# Calibration Appendix O

Methodology for

Settings: The instrument settings for the unit are summarized below:

wavelength, microns 3.26

pathlength, dial setting 14.00

slit width, mm 1

range, absorbance 1

response, seconds 1

gain high

chart speed, cm/min 1

chart volts 1

Calibrations: The Miran ® 

# was turned on and allowed to warm up for approximately

10 minutes. The cell was flushed with room air for approximately one minute. The loop

was closed, the unit was zeroed and the calibra tion series was performed as shown below.

The resultant data were plotted to obtain a calibration curve. Each observer used a

separate syringe for calibration.

Injection Calculated Absorbance

Volume Concentration 1 

# Operator 1 Operator 2 Average

(μL of liquid) (ppm) (volts) (volts) (volts)

40 1693 0.315 0.2881 0.302

60 2539 0.343 0.354 0.349

240 10156 0.605 0.588 0.597

360 15234 0.771 0.724 0.748

430 18197 0.877 0.803 0.840

1 

# Calculated Conc. (ppm): Volume injected (μL) X 1.3 mg/μL X 1000 μg/mg ÷ 5.64 L ÷ 130.5 μg/μmole

X 22.4 μL/μmole X (292 K/273 K)

where: 5.64 L = volume of Miran closed loop

1.3 mg/μL = density of test substance

130.5 μg/μmole = molecular weight of test substance

Syringes: 0-500 μL Hamilton gas-tight syringe, Model No. 1750 was used for injections.

# Huntingdon Life Sciences 09-4348 Page 289

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Miran ® 

# Calibration Appendix O

Calibration Checks: A three-point calibration check of the Miran ® 

# was performed for

each exposure prior to sampling the chambers. The parameters are shown below:

Expected Acceptable

Injection Calculated Absorbance Absorbance

Volume Concentration Reading Range

(μL of liquid) (ppm) (volts) (volts)

60 2539 0.349 0.2967 – 0.401

240 10156 0.597 0.507 – 0.687

360 15234 0.748 0.636 – 0.860

The absorbance was recorded after each inj ection. The absorbance was considered

satisfactory if it was within 15% of the original calibration series. If any of the

absorbance values fell outside the 15% range, the injection was rechecked as follows.

The volume for the value that was out of range was reinjected twice. The closer pair of

the three injections were averaged and the results were compared to the original curve. If

the average of the pair was within the 15% ra nge, the original was accepted. If the value

of the average was outside the 15% range, th e Study Director decided if a new graph was

to be prepared.

# Huntingdon Life Sciences 09-4348 Page 290

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Miran ® 

# Calibration Appendix O

Calibration Curve for

y = -50119x 3 

# + 87520x 2 

# - 16855x

R 2 

# = 0.992

0 2000 4000 6000 8000 10000 12000 14000 16000 18000 20000

0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.900 1.000

Absorbance (Volts)Concentration (ppm)

# Appendix P Page 291

# Appendix P Page 292

# Appendix P Page 293

# Appendix P Page 294

# Appendix P Page 295

# Appendix P Page 296

# Appendix P Page 297

# Huntingdon Life Sciences 09-4348 Page 298

MA-252E-93-23 Final Report

Scientists and Supervising Personnel Appendix Q

TITLE/DEPARTMENT NAME/DEGREE

SENIOR VICE PRESIDENT,

SAFETY ASSESSMENT Sylvie J Gosselin, DVM, PhD,

Diplomate ACVP

DIRECTOR, DEVELOPMENTAL AND

REPRODUCTIVE TOXICOLOGY Robert M Parker, PhD, DABT

DIRECTOR, ANALYTICAL SERVICES Barbara A Litzenberger, BS,

MT (ASCP)

DIRECTOR, TOXICOLOGY

OPERATIONS Ian Vanterpool, FIAT

STUDY DIRECTOR Gary M Hoffman, BA, DABT

REPORT WRITER Ronald L. Brzozowski, BA,

AALAS LAT

VETERINARIAN Teresa S Kusznir, VMD

MANAGER/SUPERVISOR

Rodent Toxicology

Inhalation Toxicology

Pharmacy

Necropsy and Fetal Pathology Christina Savidge, BS,

AALAS RLATG

Stuart Cracknell, CBiol, MIBiol

Michael S McCarthy

Donna G Chuddley, BS,

CLA (ASCP) HT