Cyantraniliprole Summary of Ana l ytical Chemistry and Residue Data DP#: 407961



### UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, 

### D.C. 20460

OFFICE OF CHEMICAL SAFETY

AND POLLUTION PREVENTION



## MEMORANDUM

Date: 25 January 2013



## SUBJECT: Cyantrani liprol e. Petition for the Establishment of Permanent Tolerances and

Registration for Use on 

### Brassica (cole) Leafy Vegetables (Group 5), Bulb

Vegetables (Group 3-07), Bushberries (Group 13-07B)

, Oilseed (Group 20),

Citrus Fruit (Group 10-10 ), Cucurbit Vegetables (Group 9), Fruiting Vegetables

(Group 8-10), Leafy Vegetables 

### (non-Brassica) (Group 4), Pome Fruit (Group

11-10), Stone Fruit (Group 12), Tree Nuts (Group 14), Tuberous and Corm

Vegetables (Subgroup 1C), and Food/Feed Handling Establishments. Summary

of Analytical Chemistry and Residue Data.

DP Barcodes: D407961 PC Code: 090098

Decision No.: 451670 Registration Nos.: 352

- ILA, 352-ILT, 352-ILO,

352-IAN, 352-ILI

, 352-IA T

Petition No.: IF7894 Regulatory Action: Section 3 Registration

CaseNo.: NA Risk Assessment Type: NA

TXRNo .: NA CAS No. : 736994-63-1

MRID No.: See MRID Summary Table 40 CFR: To be detennined

FROM: Steve Funk, Senior Scientist

# ~**~** l _/

Risk Assessment Branch III 

# ¥....-

Health Effects Division (7509P

THROUGH: Jeff Dawson, Acting Branch Chief

Risk Assessment Branch III

Health Effects Division (7509P)

TO: And

Thurston Morton, Senior**Chemist~·** sk Assessment Branch 

### IV

Donna Davis, Co-Chair

### ~**~** Donald Wilbur, Co-Chair

,**r~** Chemistry Science Advisory oun I hemSAC)

Health Effects Division (7509P)

John Hebert, RM07

Thomas Harris

Insecticide Branch

Regis tr ation Division (7505P)



### Page 1 

### of196 Ver.Apr.08Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP# : 407961

Summary of Submitted Res id ue Chemistry S tudies (DuPont)

OCSPP 860 MRIDNum b er Monograph Title

Series AnnexB

Guideline Reference

860

. 1300 48 119988 B

. 7

. 1.3 The Metabolism of [Car bon 14)CYAN T RANILIPROLE in Cotton

860.1300 48 119989 B

. 7

. l.4 The Metabolism of [Carbon 14)CYAN T RANILIPROLE in

Tomatoes

860.1300 48119990 B

. 7

. l.2 The Metabolism of [ C arbo n 14)C YANTRANILIPROLE in Lettuce

860.1300 48119991 B.7.2.2 Metabolism of[Carbon 14) CYA N TRANILIPROLE in the

Lac tating Goat

860.1300 48119992 B.7.2.1 The Metabolism of [Carb on 14] CYANTRANILIPROLE in the

Lay ing Hen

860.1340 48119922 8.5. 2 .1.2 Analytical Method for the D etennination of

CY ANTRANILIPROLE and M etabolites in Crops Using

LC/MS/MS

860.13 40 48 1 19923 B.5

. 2

. l Analytical Method for the D ete nnina tion of

CY ANTRANILIPROLE and Metabolites in Proc essed Fractions

Using LC / MS/MS

860.13 4 0 48119924 B.5.2.l.2 Analytical Method for the D etennination of

CY A N T RANILIPROLE and M etabol i tes in Bovine Tissues, Dairy

, and Eggs U sing LC/MS / MS

860.1340 48119925 8.5.2.1.2 Method Validation for the Analysis of CY ANTRANILIPROLE and

its Metabol ite s (IN-N7 B69

, IN-JCZ38, IN -K7H 19, IN -MYX98

, INMLA84 and IN-J9Z38) in Various Crops with LC-MS/MS

Det ect ion

86 0.1340 48119928 8.5.2.1.1 Method Assessment and Validation for the De ter m ination of

R esidues of CY ANTRANILIPRO L E i n Plants Using Multi-Residue

Method D F G S19 (LC

- MS/MS Module)

860.1340 48119929 8.5 .2

. 1.3 Multiresidue Method Tes t ing for CY ANTRANILIPROLE and Six

Metabol it es According t o t h e FDA Pes t icid e Analytical Man ua l

Volum e I (PAM

, Vol. I as Revised i n October 1999)

860.13 40 48119932 8.5.2

. 2.2 Validation of an Analytical Method f or the Detennination of

CYANTRANILIPROLE

, I N-H GW87

, IN-N7B69

, IN-K7H19

, INJCZ38, IN-MYX98, IN

- J9Z38 and IN-MLA84 in Bovine Tissu es,

Milk and Eggs

860

. 1340 48119933 8.5.2

. 2.1 Method A ssessment and Validation for the D eterm ina tion of

Residues of CY ANTRA NILIP RO LE and Metab o l ites in Animal

Tissues Using Multi-Residue Method DFG S 19 (LC/MS / MS

Modul e )

860.1340 48119934 8.5

. 2.1.2 Ind ependent Labora tory Validat i on of Ana l ytical Met hod for t h e

Detennination of CY ANT RANI LIPROLE and Metabolites in Crops

and Processed Fra ctions Us i n g LC /MS/MS

860.1340 48119998 8.5.2

. 1.2 Extraction Effic iency o f(Carbon 14 ] CYAN T RANILIPROL E from

Crops

860. 1340 48119999 8

. 5.2

. 2.1 Independent L aboratory Valida t ion for the Detennination of

Residues of CY ANTRANILIPROLE i n Ani mal Tissues Using

M ult i-R es i due Method DFG S 19 (LC

- MS / MS Modu l e)

860. 13 40 48120001 8.5

. 2

. 2.2 Extractio n E fficiency of (Carbon 14 ] CY AN T RANILIPROLE from

Muscle, Liver and Milk from Go ats and Eggs from Hens

860

. 13 40 48122526 B

. 5

. 2.1 Indep endent Lab ora to ry Validation for t h e Det enn i nation of

Residues of CY ANTRANILIPROLE in Plants using M ult i

- R es id ue

M e th od DFG S 19 (LC-MS/MS Mod ule)

860. 1340 48 122534 8.5

. 2.2 Ind ependen t Labor atory Validat i on of DuPont-18844 Method for the

Detenn i na t ion ofRes i dues ofCYA NTRA N ILIPROL E and

M e tab o l ites in Milk and Bovine T i s sues U s ing LC/MS/MS



### Page 2 of 

## 19:3Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 3 of 193 860.1380 48119993 B.7.6.2 Stability of CYANTRANILIPROLE and Metabolite/Degradation

Product Residues in Representative Crops Store d Frozen

860.1380 48120014 B.7.8.2 CYANTRANILIPROLE : Magnitude of Residues of

Cyantraniliprole ( CYANTRANILIPROLE ) and Metabolites in

Edible Tissues and Milk of Lactating Diary Cows following Dosing

with Cyantraniliprole

860.1380 48120015 B.7.8.1 Magnitude of Residues of Cyantraniliprole

( CYANTRANILIPROLE ) and Metabolites in Edible Tissues and

Eggs of Poultry following Dosing with Cyantraniliprole

860.1480 48120014 B.7.8.2 CYANTRANILIPROLE : Magnitude of Residues of

Cyantraniliprole ( CYANTRANILIPROLE ) and Metabolites in

Edible Tissues and Milk of Lactating Diary Cows following Dosing

with Cyantraniliprole

860.1480 48120015 B.7.8.1 Magnitude of Residues of Cyantraniliprole

( CYANTRANILIPROLE ) and Metabolites in Edible Tissues and

Eggs of Poultry following Dosing with Cyantraniliprole

860.1500 48120007 B.7.6.1.5

(Europe) Magnitude and Decline of CYANTRANILIPROLE and Metabolite

Residues in Protected Peppers (Fruiting Vegetables , Soanacea)

following Foliar Application of CYANTRANILIPROLE 100 g/L

OD or Drip Irrigation Application of CYANTRANILIPROLE 200

g/L SC

– Europe 2008

860.1500 48120008 B.7.1.4 Magnitude and Decline of CYANTRANILIPROLE and Metabolite

Residues in Brassica Vegetables following Foliar Applications of

CYANTRANILIPROLE 100 g/L OD, CYANTRANILIPROLE 100

g/L SE, and Soil Applications of CYANTRANILIPROLE 200 g/L

SC

860.1500 48120009 B.7.1.6 Magnitude and Decline of CYANTRANILIPROLE and Metabolite

Residues in Cucurbits (Cucumber, Cantaloupe/Muskmelon, Summer

Squash) following Foliar Applications of CYANTRANILIPROLE

100 g/L OD, and Soil Applications of CYANTRANILIPROLE 200

g/L SC

860.1500 48120010 B.7.1.5 Magnitude and Decline of CYA NTRANILIPROLE and Metabolite

Residues in Fruiting Vegetables (Tomato, Bell Pepper, Non

- Bell

Pepper) following Foliar Applications of CYANTRANILIPROLE

100 g/L OD, and Soil Applications of CYANTRANILIPROLE 200

g/L SC

860.1500 48120011 B.7.1. 3 Combined Processing and Magnitude and Decline of

CYANTRANILIPROLE and Metabolite Residues in Leafy

Vegetables following Foliar

Applications of CYANTRANILIPROLE 100 g/L OD,

CYANTRANILIPROLE 100 g/L SE and Soil Applications of

CYANTRANILIPROLE 200 g/L SC

8 60.1500 48120016 B.7.1.2 Magnitude of the Residue on Dry Bulb and Green Onion

860.1500 48120017 B.7.1.9 Combined Processing and Magnitude and Decline of

Cyantraniliprole and Metabolite Residues in Stone Fruit (Peach,

Plum, and Cherry) following Foliar App lications of

CYANTRANILIPROLE 100 g/L SE

– NAFTA 2009

860.1500 48120018 B.7.1.8 Combined Processing and Magnitude and Decline of

Cyantraniliprole and Metabolite Residues in Pome Fruit (Apple and

Pear) following Foliar Applications of CYANTRANILIPROLE 100

g/L SE

– NAFTA 2009

860.1500 48120019 B.7.1.11 Magnitude of Cyantraniliprole and Metabolite Residues in Tree Nuts

(Almonds and Pecans) following Foliar Applications (DPX

- W86

100 g/L OD and DPX

- W86 100 g/L SE) and Soil Applications

(DPX

- W86 200 g/LSC)

- NAFTA, 2009

860.1500 48120020 B.7.1.1 Combined Processing and Magnitude of Cyantraniliprole and

Metabolite Residues in Citrus Fruit (Orange, Lemon, andCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 4 of 193 Grapefruit) Following Foliar Applications of

CYANTRANILIPROLE 100 g/L SE and Systemic Delivery

Applications of CYANTRANILIPROLE 200 g/L SC.

860.1500 48120021 B.7.1.11 Combined Processing and Magnitude of Cyantraniliprole and

Metabolite Residues in Cotton following Foliar Applications of

CYANTRANILIPROLE 100 g/L OD and Soil Applications of

CYANTRANILIPROLE 200 g/L SC

- USA 2009

860.1500 48120022 B.7.1.12 Combined Processing and Magnitude of Cyantraniliprole and

Metabolite Residues in Oil Seeds (Canola and Sunflowers)

following Foliar Applications of CYANTRANILIPROLE 100 g/L

OD and Seed Treatment with 625 g/L FS

- NAFTA 2009

860.1500 48120023 B.7.1. 4 Combined Processing and Magnitude of Cyantraniliprole and

Metabolite Residues in Tuberous and Corm Vegetables following

Foliar Applications (HGW86 100 g/L OD and 100 g/L SE), Soil

Applications (200 g/L SC),and Seed Treatment (625 g/L FS)

-

NAFTA 2009

860.1500 48120034 B.7.1.10 Magnitude of Cyantraniliprole and Metabolite Residues in

Blueberries following Foliar Applications of

CYANTRANILIPROLE 100 g/L SE

- USA, 2009

860.15 00 48120037 B.7.6.1.5

(Europe) Magnitude and Decline of Cyantraniliprole and Metabolite Residues

in Protected Tomatoes and Cherry Tomatoes following Foliar

Application of CYANTRANILIPROLE 100 g/L OD and Soil

Application of DAPX

- W86 200 g/L SC

- Europe, 2009

860.1500 48120038 B.7.6.1.5

(Europe) Magnitude and Decline of Cyantraniliprole and Metabolite Residues

in Protected Peppers and Hot Peppers following Foliar Application

of CYANTRANILIPROLE 100 g/L OD and Soil Application of

DAPX

- W86 200 g/L SC

- Europe, 2009

860.1520 48120044 B.7.7.1 High Temperature Hydrolysis of [Carbon 14]

-

CYANTRANILIPROLE in Buffered Aqueous Solution at pH 4, 5,

and 6

860.1520 48120011 B.7.1.5

(NAFTA) Combined Processing and Magnitude and Decline of

CYANTRANILIPROLE and Metabolite Residues in Leafy

Vegetables following Foliar

Applications of CYANTRANILIPROLE 100 g/L OD,

CYANTRANILIPROLE 100 g/L SE and Soil Applications of

CYANTRANILIPROLE 200 g/L SC

8 60.15 20 48120012 B.7.1.6

(NAFTA) Magnitude of CYANTRANILIPROLE and Metabolite Residues in

Processed Fractions of Tomatoes following Foliar Applications of

CYANTRANILIPROLE 100 g/L OD

– U.S. 2008

860.1520 48120017 B.7.1.11

(NAFTA) Combined Processing and Magnitude and Decline of

Cyantraniliprole and Metabolite Residues in Stone Fruit (Peach,

Plum, and Cherry) following Foliar Applications of

CYANTRANILIPROLE 100 g/L SE

– NAFTA 2009

860.1520 48120018 B.7.1.10

(NAFTA) Combined Proces sing and Magnitude and Decline of

Cyantraniliprole and Metabolite Residues in Pome Fruit (Apple and

Pear) following Foliar Applications of CYANTRANILIPROLE 100

g/L SE

– NAFTA 2009

860.1520 48120020 B.7.1.8

(NAFTA Combined Processing and Magnitude of Cyant raniliprole and

Metabolite Residues in Citrus Fruit (Orange, Lemon, and

Grapefruit) Following Foliar Applications of

CYANTRANILIPROLE 100 g/L SE and Systemic Delivery

Applications of CYANTRANILIPROLE 200 g/L SC.

860.1520 48120021 B.7.1.12

(NAFTA) Combined Processing and Magnitude of Cyantraniliprole and

Metabolite Residues in Cotton following Foliar Applications of

CYANTRANILIPROLE 100 g/L OD and Soil Applications of

CYANTRANILIPROLE 200 g/L SC

- USA 2009

860.1520 48120023 B.7.1.4 Combined Processing and Magnitude of Cyantraniliprole andCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 5 of 193 (NAFTA) Metabolite Residues in Tuberous and Corm Vegetables following

Foliar Applications (HGW86 100 g/L OD and 100 g/L SE), Soil

Applications (200 g/L SC),and Seed Treatment (625 g/L FS)

-

NAFTA 2009

860.1 850 48119986 B.7.9.2 CYANTRANILIPROLE Pilot Confined Crop Rotation Study

(Wheat, Soybeans, and Red Beets)

860.1850 48119987 B.7.9.2 Confined Rotational Crop Study Using [Carbon 14]

CYANTRANILIPROLE

860.1900 48119995 B.7.9.3.2 Field Crop Rotation Study with CYANTRANILIPROLE 10OD

Insecticide

- EPA Region 10, USA, 2006

860.1900 48119996 B.7.9.3.1 Field Crop Rotation Study with CYANTRANILIPROLE 10OD

Insecticide

- EPA Region 11, USA, 2006

860.1900 48120005 B.7.9.3.3 Magnitude of CYANTRANILIPROLE and Metabolite and/or

Degradation Product Residues in Field Rotational Crops following

Application of CYANTRANILIPROLE 100 g/L OD Insecticide

- Ontario, Canada Location 2008/2009

860.1900 48120035 B.7.9.3.4 Magnitude of Cyantraniliprole a nd Metabolite Residues in Field

Rotational Crops following Bare Ground Applications of DPX

- 100

G/L SE

- USA, 2009

860.1900 48519713 B.7.9.3.5 Magnitude of Cyantraniliprole and Metabolite Residues in Field

Rotational Crops following Bare Ground Applications of DPX

- 100

g/L SE

- USA and Canada, 2010



### Executive Summary

Cyantraniliprole is a second

- generation

, systemic insecticide belonging to the diamide class of

chemistry, mobile in both the xylem and phloem. The mode of action is through its binding with

insect ryanodine receptors. This causes muscle contraction, which leads to paralysis and eventual

death o f the insect. It is structurally identical to chlorantraniliprole except with the substitution of

the chlorine atom with a cyano group.

This is the first registration and tolerance request for cyantranilirprole under a global joint review

(Australia, Cana da, France, United Kingdom, USA).

DuPont has proposed tolerances for residues of the insecticide cyantraniliprole, including its

metabolites and degradates, in or on the commodities below. DuPont has stated that c ompliance

with the tolerance levels specif ied below is to be determined by measuring cyantraniliprole

, 3

-

bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

pyrazole

- 5

- carboxamide i n or on the following commoditi es:

Almond, hulls

................................ ................................ ................................ .......................... 3 0 ppm

Berries and small fruits, bushberries (crop subgroup 13

- 07B)

................................ ................. 4 ppm

Brassica (cole) leafy vegetables, head and stem Brassica (crop subgroup 5A)

....................... 2 ppm

Brassica (cole) leafy vegetables, leafy Brassica greens (crop subgroup 5B)

.......................... 3 0 ppm

Bulb vegetables, onion, bulb (crop su bgroup 3

- 07A)

................................ ........................... 0.0 4 ppm

B ulb vegetables, onion, green (crop subgroup 3

- 07 B )

................................ ............................. 8 ppm

Cattle, fat

................................ ................................ ................................ .............................. 0.0 1 ppm

Cattle, liver

................................ ................................ ................................ ........................... 0.0 4 ppm

Cattle, meat

................................ ................................ ................................ .......................... 0.0 1 ppm

Cattle, meat byproducts, except liver

................................ ................................ ................... 0.0 1 ppm

Cherries

................................ ................................ ................................ ................................ .... 6 ppmCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 6 of 193 Citrus fruits (crop group 10

- 10)

................................ ................................ ............................. 0 .7 ppm

Cotton, gin byproduct

................................ ................................ ................................ .............. 10 ppm

Cucurbit vegetables (crop group 9)

................................ ................................ ........................ 0. 3 ppm

Fruiting vegetables (crop group 8

- 10)

................................ ................................ ...................... 2 ppm

Goat , fat

................................ ................................ ................................ ................................ 0.0 1 ppm

Goat , liver

................................ ................................ ................................ ............................. 0.0 4 ppm

Goat , meat

................................ ................................ ................................ ............................ 0.0 1 ppm

Goat , meat byproducts, except liver

................................ ................................ ..................... 0.0 1 ppm

Hog , fat

................................ ................................ ................................ ................................ . 0.0 1 ppm

Hog , liver

................................ ................................ ................................ .............................. 0.0 4 ppm

Hog , meat

................................ ................................ ................................ ............................. 0.0 1 ppm

Hog , meat byproducts, except liver

................................ ................................ ...................... 0.0 1 ppm

Horse , fat

................................ ................................ ................................ .............................. 0.0 1 ppm

Horse , liver

................................ ................................ ................................ ........................... 0.0 4 ppm

Horse , meat

................................ ................................ ................................ .......................... 0.0 1 ppm

Horse , meat byproducts, except liver

................................ ................................ ................... 0.0 1 ppm

Leafy vegetables (except Brassica vegetables) (crop group 4)

................................ ............... 15 ppm

Milk

................................ ................................ ................................ ................................ ...... 0.0 1 p pm

Milk fat

................................ ................................ ................................ ................................ . 0.04 ppm

Oilseeds, except cotton byproduct (crop group 20)

................................ ................................ .. 1 ppm

Pome fruits (crop group 11

- 10)

................................ ................................ .............................. 0. 8 ppm

Root and tuber vegetables, tuberous and corm vegetables (crop subgroup 1C)

................... 0. 15 ppm

Sheep, fat

................................ ................................ ................................ .............................. 0.0 1 ppm

Sheep, liver

................................ ................................ ................................ ........................... 0.0 4 ppm

Sheep, meat

................................ ................................ ................................ .......................... 0.0 1 ppm

Sheep, meat byproducts, except liver

................................ ................................ ................... 0.0 1 ppm

Stone fruits, except cherries (crop group 12)

................................ ................................ ......... 1. 5 ppm

Tree nuts, except almond hulls (crop group 14)

................................ ................................ ... 0.06 ppm



### Also, the registrant has proposed tolerances for residues of the insecticide cyantraniliprole,

including its metabolites and degradates, in or on the [processed] commodities below. DuPont

has stated that c ompliance with the tolerance levels specified below is to be determined by

measuring the sum of cyan traniliprole

, 3

- bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

6

- [(methylamino)carbonyl]phenyl]

- 1 H

- pyrazole

- 5

- carboxamide and its metabolite

, IN

- J9Z38, 2

- [3

- bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1 H

- pyrazole

- 5

- yl]

- 3,4

- dihydro

- 3,8

- dimethyl

- 4

- oxo

- 6

- quinazolineca rbonitrile, i n or on the following commoditi es , when present therein as a result of

the application of the pesticide to growing crops listed as follows :

Citrus, oil

................................ ................................ ................................ ................................ .. 6 ppm

Citrus, raw peel

................................ ................................ ................................ ........................ 2 ppm

Potato, wet peel

................................ ................................ ................................ ...................... 0. 3 ppm



### Furthermore, the registrant has proposed tolerances for indirect or inadvertent residues of the

insecticide cyantraniliprole, including its metabolites and degradates, in or on the commodities

below. DuPont has stated that c ompliance with the tolerance levels specified below is to be

det ermined by measuring cyantraniliprole

, 3

- bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

- pyrazole

- 5

- carboxamide, i n or on the following

commoditi es , when present therein as a result of the application of the pesticide to growing crops

listed as follows :

Foliage of legume vegetables (crop group 7), forage

................................ ........................... 0.15 ppm

Foliage of legume vegetables (crop group 7), hay

................................ ................................ . 0.6 ppm

Forage, fod d er and straw of cereal gr ai ns (crop group 16)), forage

................................ ..... 0.06 ppm

Forage, fo d der and straw of cereal gr ai ns (crop group 16)), hay and straw

......................... 0.15 ppmCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 7 of 193 Grass forage , fodder, and hay (crop group 17), forage

................................ ......................... 0. 06 ppm

Grass forage, fodder, and hay (crop group 17), hay

................................ ............................. 0.15 ppm

Leaves of root and tuber vegetables (human food or animal feed) (crop grou p 2)

............... 0. 04 ppm

Nongrass animal feeds (forage, fodder, straw, and hay) (crop group 18), forage

................ 0.06 ppm

Nongrass animal feeds (forage, fodder, straw, and hay) (crop group 18), hay

..................... 0.15 ppm

Peanut hay

................................ ................................ ................................ ............................ 0.03 ppm

Root and tuber vegetables, root vegetables (crop subgroup 1A)

................................ .......... 0. 0 3 ppm



### Plant metabolism studies were conducted in rice, lettuce, cotton, and tomato, representing

cereals, leafy, oilseeds, and fruit groups. Results indicate that plant metabolism is similar

following foliar and soil application. The metabolic fate of this insecticide is complex with the

formation of many low level metabolites. However, cyantraniliprole is the major component in

rice, lettuce, cotton, and tomato. Results in crop field trials confirm that the parent compound is

the pred ominant residue.

In rotational crops, parent is the major residue present in soybean, wheat gr ai n, lettuce, and beet

root. As in primary crops, there exist many minor metabolites. In livestock feed stuffs such as

wheat forage, hay, straw, and soybean foli age, parent plus one or two metabolites (IN

- J9Z38, IN

JSE76, and/or IN

- MLA84) are present as major residues.

The metabolism in livestock is extensive and the amount of unchanged parent present in milk,

eggs, and tissues is matrix

- dependent. The major com ponents present in various tissues include a

combination of parent, IN

- N7B69, IN

- J9Z38, IN

- MLA

- 84, and/or IN

- MYX98.

The results of processing studies indicate the formation of three major degradates (IN

- J9Z38, IN

N5M09, and IN

- F6L99) under conditions of h eat and/or hydrolysis. All three degradates are

present in apple sauce, whereas only IN

- J9Z38 is present in cooked spinach, orange oil, tomato

paste, cottonseed oil, and cottonseed meal.

S torage stability data have been submitted for residues of cyantrani liprole and various

metabolites in five categories of plant commodity matrices and in milk and eggs. The intervals

of demonstrated storage stability cover the actual intervals of frozen storage of plant and

livestock commodity samples from the crop field trials, processing studies, and livestock feeding

studies. No corrections for loss during storage are needed for any plant or livestock commodity

for purposes of tolerance considerations.

Based on the livestock metabolism studies which did not show a cha nge in the metabolic profile

after 10 months of frozen storage for eggs, muscle and fat of hens and 6 months of frozen storage

for milk, fat, kidney and liver of goats, and since the egg, milk, and tissue samples from the

livestock feeding studies were sto red frozen and analyzed within 30 days of collection, HED

concludes that storage stability data for the livestock matrices are unnecessary

.

Adequate field trial data are av ai lable to support the proposed uses on Brassica (cole) Leafy

Vegetables (Group 5), Bulb Vegetables (Group 3

- 07), Bushberries (Group 13

- 07B), Oilseed

(Group 20), Citrus Fruit (Group 10

- 10), Cucurbit Vegetables (Group 9), Fruiting Vegetables

(Group 8

- 10), Leafy Vegetables (non

- Brassica ) (Group 4), Pome Fruit (Group 11

- 10), Stone

Fruit (Group 12

- 12 ), Tree Nuts (Group 14), and Tuberous and Corm Vegetables (Subgroup 1C).

There are no data to support use in food/feed handling, storage, and transport facilities.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 8 of 193 M ethods for measuring cyantraniliprole include EU multi

- residue method DFG S19 (LC/MS/MS

module, Dupont

- 21328) and NAFTA LC/MS/MS 1187 & 1552 methods. These methods utilize

two mass transitions per analyte , which is sufficiently specific so that confirmatory methods are

not required. NAFTA method 1187 has been independently val idated with a limit of

quantification ( LOQ ) of 0.01 ppm parent and its metabolites in almond, onion, tomato paste and

sun dried tomatoes. Method 1552 has been independently validated with an LOQ of 0.01 ppm

parent and its metabolites in milk, muscle and ki dney. DFG S19 has been independently

validated with a n LOQ of 0.01 ppm parent for cereals and dry products, matrices with high water

content, acidic matrices and fatty product.

Cyantraniliprole is not recovered using the FDA multi

- residue method s

. Cyantr aniliprole is

successfully recovered and quantitiated by the European DFG method.

Processing studies have been submitted for several crops including potato, tomato, orange, apple,

plum, and cottonseed. N o tolerances are needed for most processed commodities, as the

cyantraniliprole concentration did not increase from the raw agricultural commodity (RAC) to

the processed commodity. Cyantraniliprole did concentrate in tomato dry pomace, sundried

tomatoes, orange oil, apple puree, ap ple dry pomace (but not wet) , and dried plums. Dried plums

and citrus oil are recognized processed commodities for purposes of tolerances.

Adequate cattle and poultry feeding studies have been submitted. The data indicate that

tolerances for milk and th e fat, meat, and meat byproducts of cattle, goat, horse, and sheep are

needed to support the proposed cyantraniliprole uses , but tolerances are not required for hogs and

poultry commoditie s

.

The confined rotational crop studies are adequate. A dequate l imited and extended field

rotational crop studies have been submitted. The studies indicate non

- quantifiable levels of

parent residues in/on cereal gr ai ns, soybean seed, and legumes, and quantifiable levels in leafy

vegetables , foliage of legume vegetable s, and some root commodities

, at a plantback interval

(PBI) of 30 days. T he studies also indicate quantifiable levels of parent residues in livestock feed

stuff s such as alfalfa, grass hay, and soybean hay at intervals up to 365 days

. Inadvertent or

indirect tolerances for these rotational crops are needed at a PBI of 30 days

.

Currently there are no Codex MRLs/tolerances for cyantraniliprole. Therefore, there are no

issues with harmonization of tolerances. Canadian MRLs are being proposed concur rently with

US tolerances and are harmonized for primary crop commodities and livestock commodities

except fruiting vegetables

. The US fruiting vegetables tolerance is harmonized with the

European Union.

Regulatory Recommendations and Residue Chemistry D eficiencies

HED has examined the residue chemistry database for cyantraniliprole . Pending submission of a

revised Section B (see requirements under Directions for Use)

, a revised Section F (see

requirements under Proposed Tolerances and Table 140 ), and a nalytical reference standard s (see

requirements under Submittal of Analytical Reference Standards), there are no residue chemistry

issues that would preclude granting Section 3 registration for the requested crop commodity uses

of cyantraniliprole (except food/feed establishment uses)

, or establishment of tolerances forCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 9 of 193 residues of cyantraniliprole . It is recommended that tolerances be established for residues of the

insecticide cyantraniliprole (3

- bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methy l

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

- pyrazole

- 5

- carboxamide), including its metabolites and

degradates, in or on the plant commodities specified below and detailed in Table 140

. Compliance with the tolerance levels specified is to be determined by measur ing only

cyantraniliprole in or on the commodity.

Almond, hulls

................................ ................................ ................................ ......................... 8 .0 ppm

Bushberry subgroup 13

- 07B

................................ ................................ ................................ .. 4 .0 ppm

Brassica

, head and stem, subgroup 5A

................................ ................................ ................... 3 .0 ppm

Brassica

, leafy greens, subgroup 5B

................................ ................................ ....................... 30 ppm

Onion, bulb, subgroup 3

- 07A

................................ ................................ ............................... 0.0 4 ppm

Onion, green, subgroup 3

- 07B

................................ ................................ ............................... 8 .0 ppm

Cherry subgroup 12

- 12A

................................ ................................ ................................ ........ 6 .0 ppm

Fruit, citrus group 10

- 10

................................ ................................ ................................ ....... 0.7 0 ppm

Citrus, oil

................................ ................................ ................................ ................................ 2.4 ppm

Cotton, gin byproducts

................................ ................................ ................................ ............ 10 ppm

Vegetable, cucurbit, group 9

................................ ................................ ................................ 0.4 0 ppm

Vegetable, fruiting, group 8

- 10

................................ ................................ .............................. 2 .0 ppm

Vegetable, leafy, except Brassica

, group 4

................................ ................................ ............. 20 ppm

Oilseed group 20

................................ ................................ ................................ .................... 1.5 ppm

Fruit, pome, group 11

- 10

................................ ................................ ................................ ........ 1.5 ppm

Vegetable, tuberous and corm, subgroup 1C

................................ ................................ ........ 0.15 ppm

Peach subgroup 12

- 12B

................................ ................................ ................................ .......... 1.5 ppm

Plum subgroup 12

- 12C

................................ ................................ ................................ .......... 0 .5 0 ppm

Nut, tree, group 14

................................ ................................ ................................ ................ 0.04 ppm



### It is recommended that tolerances be established for residues of the insecticide cyantraniliprole

(3

- bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

pyrazole

- 5

- carboxamide), including its metabolites and degradates, i n or on the livestock

commodities below and in Table 140 . Compliance with the tolerance levels specified below is

to be determined by measuring only cyantraniliprole in or on the commodity.

Cattle, fat

................................ ................................ ................................ .............................. 0. 0 1 ppm

Cattle, meat

................................ ................................ ................................ .......................... 0. 0 1 ppm

Cattle, meat byproducts

................................ ................................ ................................ ........ 0.01 ppm

Goat, fat

................................ ................................ ................................ ................................ 0. 0 1 ppm

Goat, meat

................................ ................................ ................................ ............................ 0. 0 1 ppm

Goat, meat byproduct s

................................ ................................ ................................ .......... 0.0 1 ppm

Horse, fat

................................ ................................ ................................ .............................. 0.0 1 ppm

Horse, meat

................................ ................................ ................................ .......................... 0. 01 ppm

Horse, meat byproducts

................................ ................................ ................................ ........ 0.01 ppm

Milk

................................ ................................ ................................ ................................ ...... 0 .0 1 ppm

Sheep, fat

................................ ................................ ................................ .............................. 0.0 1 ppm

Sheep, meat

................................ ................................ ................................ .......................... 0. 0 1 ppm

Sheep, meat byproduct s

................................ ................................ ................................ ........ 0.01 ppm



### It is recommended that indirect or inadvertent tolerances be established for residues of

insecticide cyantraniliprole (3

- bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

- pyrazole

- 5

- carboxamide), including its metabolites and

degradates, in or on the commodities below and in Table 140 . Compliance with the toleranceCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 10 of 193 levels specified below is to be determined by measuring only cyantraniliprole in or on the

commodity.

Vegetable, foliage of legume, group 7

................................ ................................ ................. 0. 7 0 ppm

Gr ai n, ce real, forage, fodder and straw, group 16

................................ ................................ 0.5 0 ppm

Grass, forage, fodder and hay, group 17

................................ ................................ .............. 0.5 0 ppm

Vegetable, leaves of root and tuber, group 2

................................ ................................ ........ 0.04 ppm

Animal feed, nongrass, group 18

................................ ................................ .......................... 0.2 0 ppm

Peanut, hay

................................ ................................ ................................ ........................... 0.01 ppm

Vegetable, root, subgroup 1A

................................ ................................ ............................... 0.02 ppm



### A human health risk assessment is forthcoming.

Pre

- Registration Data Needs

860.1200 Directions for Use

The use in food/feed handling establishments is not supported by the required studies of

OCCPS Guideline 860.1460. Therefore, all uses related to t reatment of food and feed

handling/storage/ transportation are not allowed. This applies specifically to DuPont

HGW86 suspension concentrate 352

- I AI

.

The rotational crop restrictions must be amended, as follows:

(1) Cereal gr ai ns (crop group 15) may not be planted immediately after use of

cyantraniliprole. Cereal gr ai n (crop group 15) must have a 30 day plantback interval

(PBI). This is to be consistent with the 30 day PBI for crop group 16 (forage, fodder,

and straw of cereal grains).

(2) Legu me vegetables (crop group 6) may not be planted immediately after use of

cyantraniliprole. Legume vegetables (crop group 6) must have a 30 day PBI. This is

to be consistent with the 30 day PBI for crop group7 (foliage of legume vegetables).

(3) Tuberous and corm vegetables (subgroup 1C) may not be planted immediately after

use of cyantraniliprole. The subgroup may be expanded to the entire tuberous and

corm vegetables group with a PBI of 30 days. Although a primary crop use exists for

subgroup 1C for the root s, the PBI of 30 days is needed to cover the foliage (under

crop group 2).

860.1550 Proposed Tolerances

A revised Section F should be submitted reflecting the tolerances and commodity

definitions that appear in Table 140 . The proposed tolerances for almond hulls,

Brassica head and stem (crop subgroup 5 A), cattle liver, citrus raw peel, cucurbit

vegetables (crop group 9), goat liver, sheep liver, horse liver, h og fat, hog liver, hot

meat, hog byproducts, leafy vegetables except Brassica (crop group 4), milk fat,

oilseeds (crop group 20), pome fruits (crop group 11

- 10), potato wet peel, stone fruits

except cherry (crop group 12

- 12 ), citrus oil, and tree nuts (crop group 14), need to be

modified

. The recommended primary pla nt commodity tolerances are derived from

use of the Organization of Economic Cooperation and Development (OECD)

statistical calculation procedure. The livestock commodity tolerances are derivedCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 11 of 193 from consideration of the maximum reasonalbly balanced livesto ck diets and the

livestock feeding studies. The recommended processed commodity tolerance is based

on the median processing factor and the HAFT (highest average field trial). The

proposed tolerances for inadvertent residues for group 7, group 16, group 1 7, group

18, peatnut hay, and subgroup 1A

, with a PHI of 30 days, need to be revised to

reflect the recommended tolerances based on the use of the OECD statistical

calculation procedure. These calculation procedures explain the differences observed

in pr oposed and recommended tolerances.

. For some crop groups, more than one

tolerance was proposed for various components of the group, for example, crop group

6 (forage, fodder, and straw of cereal grains). Only one tolerance is possible per

group, and th e tolerance is based on the member commodity with the highest residue

levels.

860.1650 Sub mittal of Analytical Reference Standards

analytical reference standards for cyantraniliprole must be submitted to th e

Analytical Chemistry Lab, which is located at Fort Me ade, to the attention of either

Theresa Cole or Thuy Nygen at the following address:

USEPA

National Pesticide Standards Repository/Analytical Chemistry

Branch/OPP

701 Mapes Road

Fort George G. Meade, MD 20755

- 5350

Condition of Registration Data Needs

There are no conditions of registration data needs.

Background

The chemical names and structures of major cyantraniliprole metabolites are presented in

Appendix I

.

Table 1. Chemical Nomenclature

.

Chemical structure

Common name Cyantraniliprole

Company experimental name C yantraniliprole ; DPX

- HGW86Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 12 of 193 Table 1. Chemical Nomenclature

.

IUPAC name 3

- B romo

- 1

- (3

- chloro

- 2

- pyrid yl)

- 4 ’

- cyano

- 2 ’

- methyl

- 6 ’

- (methyl carb am o yl)

- pyrazole

- 5

- carboxanil ide

CAS name 3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

- pyrazole

- 5

- carboxamide

CAS registry number 736994

- 63

- 1

Formula C 19 H 14 BrClN 6 O 2

End

- use product (EP) 352

- IAG, 18.66% SX; 352

- I AI , 18.66% SC; 352

- IAN, 18.66% SC; 352

- IAT,

50% FS; 352

- ILT, 50% FS; 352

- ILD, 10.2% SE; 352

- ILT, 10.26% OD.

Table 2. Physicochemical Properties of the Technical Grade of Cyantraniliprole

.

Parameter Value Reference

Physical state White fine powder solid MRID 48208438

Melting point 224 oC MRID 48208438

Density 1.4965 g/cm 3 at 20 oC MRID 48208438

Water solubility (20 °C) 14.24 mg/L MRID 48208438

Solvent solubility (g/L at 20 °C) Acetone 6.54

Ethyl acetate 1.96

Dichloromethane 5.05

Toluene 0.576

n

- Octanol 0.79

Methanol 4.73

o

- Xylene 0.29

Acetonitrile 2.45 MRID 48208438

Vapor pressure 5.13 x 10

- 15 Pa at 20 oC MRID 48208438

Henry’s Law Constant ( at 20 °C ) pH7 = 1.7 x 10

- 18 atmosphere.m 3 /mole MRID 48208438

Octanol/water partition coefficient Log Kow = 1.94 + 0.11 at 20 oC MRID 48208438

Hydrolysis and photolysis pH 4: Stable

pH 7: Stable

pH 9: Hydrolyzed very rapidly with a half

- life

of <1 day. On e major metabolite was

observed, IN

- J9Z38.

Photolysis (DT 50 ) : 0. 233 days at 40 o latitude in

the summer to 4.12 days at 60 o latitude in the

winter MRID 48208438

Dissociation constant (pK

a ) 8.80 + 1.38 at 20 oC MRID 48208438



### 860.1200 Directions for Use

The petitioner has submitted draft specimen label s for the end

- use product s cont ai ning

cyantraniliprole listed in Table 3 . The product s include suspoemulsion ( SE ) , suspension

concentrate ( SC )

, flowable suspension ( FS )

, and oil dispersion ( OD ) formulation s from DuPont

. A summary of the proposed use patterns on food and feed crops is det ai led in Table 4

.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 13 of 193 Table 3. Summary of Proposed End

- Use Products for Agricultural and Food/Feed Use

.

Trade Name File

Symbol

No. ai (% of

formulation) Formulation

Type Target Crops Label

Date

DuPont HGW86

GH & N Insect

Control 352

- IAG 18.66

(1.67 lb

ai /gallon) SC

(suspension

concentrate) Vegetable transplants in greenhouses

(fruiting vegetables, leafy vegetables,

tuberous and corm vegetables) -

DuPont HGW86

SC Insect

Control 352

- I AI 18.66

(1.67 lb

ai /gallon) SC

(suspension

concentrate) Residential, Commercial , and Livestock

buildings and transport, including food and

feed. -

DuPont

VerimarkTM

Insect Control 352

- IAN 18.66

(1.67 lb

ai /gallon) SC

(suspension

concentrate) Brassica vegetables

Cucurbit vegetables

Fruiting vegetables

Leafy vegetables

Tuberous and corm vegetables

Citrus -

DuPont

DermacorTM Z

103 Insecticide

Seed Treatment 352

- ILI 50.

(5.21 lb

ai /gallon) FS

(flowable

suspension) Rapeseed (including canola)

Mustard seed -

DuPont ExirelTM

Insect Control 352

- ILO 10.20

(0.83 lb

ai /gallon) SE

(suspoemuls

ion or oil in

water) Brassica (Cole) leafy vegetables

Bulb vegetables

Cucurbit vegetables

Fruiting vegetables

Commercial greenhouse grown (to

harvest): eggplant, pepper, tomato

Leafy vegetables

Bushberries (subgroup 13

- 7B)

Citrus fruit

Pome fruit

Stone fruit

Tree nuts -

DuPont

BeneviaTM

Insect Control 352

- ILT 10.26%

(0.83 lb

ai /gallon) OD

(oil

dispersion) Bulb vegetables

Cotton

Oil seed crops

Tuberous and corm vegetables

Tree nuts -

Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

Brassica (cole) Leafy Vegetables (Group 5)

Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.045

–

0.133 N/S 0.4 5 1 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 14 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

non

- ionics;

organosilicones/organosilicat

es).

For diamondback moth, do

NOT apply more than twice

to any generation within a 30

day period nor more than 6

applications per year.

Soil at

Planting (in

furrow spray,

transplant

water

treatment, hill

drench,

surface band,

soil shank

injection) 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.065

–

0.130 1 0.176 at

- plant;

0.4 all N/A N/A Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

ai rblast applications.

For diamondback moth, do

NOT apply(all

cyantraniliprole

formulations) more than

twice to any generation

within a 30 day period nor

more than 6 applications per

year.

Bu lb Vegetables (Group 3

- 07)

Foliar

(ground,

aerial)

Chemigation

(overhead

only) 10.26% OD

(BeneviaTM) 0.088

–

0.133 N/S 0.4 5 1 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es) Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.088

–

0.133 N/S 0.4 5 1

Bushberries (Group 13

- 07B)

Foliar

(ground,

aerial) 10.20% SE

( susp

emulsion )

(ExirelTM) 0.065

–

0.133 N/S 0.4 5 3 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosili cones/organosilicat

es)

Do NOT apply dilute

applications of more than

200 gallons per acre.

Do NOT apply less than 30

gallons of water per acre

Canola (Rapeseed)

Seed

treatment 50% FS

(flowable

suspension) 0.15

–

1.0 lb

ai /cwt 1 0.4 all N/A N/A Apply as a water

- based

slurry.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 15 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

Dermacor®

Z

- 103

Citrus Fruit (Group 10

- 10)

Foliar

(ground,

aerial) 10.20% SE

( suspo

emulsion )

(ExirelTM) 0.088

–

0.133 N/S 0.4 7 1 Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es).

For best results, use 100

– 150 gallons of water per acre

with ai rblast equipment, but

not less than 30 gallons of

water per acre.

Do NOT apply less than 2

gallons of water per acre

with fogger equipment for

Asian citrus psyllid contro.

Soil drench or

microsprinkler

chemigation 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.261

–

0.391 1 0.4 all N/A N/A For use on citrus nursery

stock (trees less than 4 years

old).

Do NOT make aerial or

ai rblast applications.

Soil drench should be in a

minimum of 1⁄2 to 1 pint of

water per tree distributed

evenly around the tree root

zone.

Microsprinkler chemigation

application pattern must

include a majority of the

tree’s root zone.

Cotton

Foliar

(ground,

aerial) 10.26% OD

(BeneviaTM) 0.045

–

0.133 N/S 0.4 7 7` Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)

Cucurbit Vegetables (Group 9)

Foliar

(ground,

aerial) 10.20% SE

( suspo

emulsion )

(ExirelTM) 0.045

–

0.133 N/S 0.4 1 1 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 16 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)

Soil at

Planting (in

furrow spray,

transplant

water

treatment, hill

drench,

surface band,

soil shank

injection) 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.065

–

0.176 1 0.176

0.4 all N/A 1 Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

ai rblast applications.

Drip

chemigation 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.065

–

0.130 2

(1 if at

plant

application

made) 0.26

0.4 all 10 1 Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

ai rblast applications.

Must be applied uniformly in

the root zone.

Make application up to peak

bloom crop stage, about 40

days after crop emergence or

transplant

Fruiting Vegetables (Group 8

- 10)

Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.045

–

0.133 N/S 0.4 1 5 May be used in greenhouses

for the production of

eggplant, pepper, tomato.

Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)

Foliar

(greenhouse) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.088

–

0.133 N/S 0.4 1 1 Eggplant, pepper, tomato.

Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 17 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

Soil at

Planting (in

furrow spray,

transplant

water

treatment, hill

drench,

surface band,

soil shank

injection) 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.065

–

0.176 1 0.176

0.4 all N/A N/A Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

ai rblast applications.

Drip

chemigation 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.065

–

0.130 2

(1 if at

plant

application

made ) 0.26

0.4 all 10 1 Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

ai rblast applications.

Must be applied uniformly in

the root zone.

Leafy Vegetables (n o n

- Brassica ) (Group 4)

Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.045

–

0.133 N/S 0.4 5 1 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)

Soil at

Planting (in

furrow spray,

transplant

water

treatment, hill

drench,

surface band,

soil shank

injection) 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.065

–

0.176 1 0.176

0.4 all N/A N/A Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

a i rblast applications.

Mustard Seed

Seed

treatment 50% FS

(flowable

suspension)

Dermacor®

Z

- 103 0.15

–

1.0 lb

ai /cwt 1 0.4 all 7 7 Apply as a water

- based

slurry.

Oil Seed Crops (Group 20) (see also rapeseed)

Foliar

(ground,

aerial) 10.26% OD

(BeneviaTM) 0.045

–

0.133 N/S 0.4 7 7 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 18 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

petroleum oil concentrates:

non

- ionics;

organosilicones/organosi licat

es)

Pome Fruit (Group 11

- 10)

Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.055

–

0.133 N/S 0.4 7 3 Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es).

Do NOT apply dilute

applications of more than

200 gallons of water per

acre

. Do NOT apply less than 30

gallons of water per acre,

Rapeseed

Seed

treatment 50% FS

(flowable

suspension)

Dermacor®

Z

- 103 0.15

–

1.0 lb

ai /cwt 1 0.4 all 7 7 Apply as a water

- based

slurry.

Stone Fruit (Group 12)

Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.065

–

0.133 N/S 0.4 7 3 Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es).

Do NOT apply dilute

applications of more tha n

200 gallons of water per

ac re.

Do NOT apply less than 30

gallons of water per acre,

Tree Nuts (Group 14) and Pistachio

Foliar

(ground,

aerial) 10.26% OD

(BeneviaTM) 0.055

0.133 N/S 0.4 7

(14

coddli

ng

moth) 5 Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)

Do NOT apply dilute Foliar

(ground,

aerial) 10.20% SE

( suspoemuls

ion )

(ExirelTM) 0.055

0.133 N/S 0.4 7

(14

coddli

ng

moth) 5Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 19 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

application of more than 300

gallons of water per acre.

Do NOT apply less than 30

gallons of water per acre.

Tuberous and Corm Vegetables (Subgroup 1C)

Foliar

(ground,

aerial)

Chemigation

(overhead

only) 10.26% OD

(BeneviaTM) 0.045

–

0.133 N/S 0.4 5 7 Do not USE in greenhouses

for transplant production;

Do NOT apply with drip

irrigation;

Do NOT apply to soil.

Adjuvants may be used

(methylated seed oils;

petroleum oil concentrates:

non

- ionics;

organosilicones/organosilicat

es)

Do NOT apply more than

twice to a generation of

Colorado potato bettle.

For potato tuberworm, avoid

treating successive

ge nerations with the same

mode of action pesticide.

Soil at

Planting (in

furrow spray,

transplant

water

treatment, hill

drench,

surface band,

soil shank

injection) 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.088

-

0.176 1 0.176

0.4 all N/A N/A Do not USE in greenhouses

for transplant production;

Do NOT make aerial or

ai rblast applications.

Potato seed

piece

treatment 18.66% SC

(suspension

concentrate)

(Verimark

TM) 0.131

–

0.176

[0.006

–

0.010 lb

ai / cwt] 1 0.176

0.4 all N/A N/A Apply as a water

- based

slurry prior to planting. Use

approved dye or colorant.

Food and Feed Handling Establishments

Crack and

crevice,

including

indoor areas

with exposed

food/feed

present.

For use in

residential

buildings,

schools,

institutional,

commercial, 18.66% SC

(suspension

concentrate)

HGW86 0.018

–

0.035 lb

ai /1000

sq ft As needed Not specified 7 N/A Avoid spraying food and

feed handling equipment.

Do not apply to areas that are

routinely washed such as

cracks and crevices in tops of

tables, food/feed preparation

and prepared food holding

surfaces.

Do not apply a broadcast or

general surface application to

interior surfaces of

residential or commercial

structures.

Do not apply this product toCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 20 of 193 Table 4 . Summary of Proposed Directions for Use of Cyantraniliprole (DuPont)

Applic ation

Timing, Type,

and Equip. Formulation Applic.

Rate

(lb ai /A) Max. No.

Applic. per

Season Max. Seasonal

Applic. Rate

(lb ai /A) RTI

(days) PHI

(days) Use Directions and

Limitations

agricultural

and industrial

facilities

(including

warehouses,

apartments,

supermarkets,

restaurants,

hospitals, and

food

handling/stora

ge/processing

establishments

, animal

production

facilities,

feedlots,

broiler houses,

livestock

barns, pet

kennels) and

transportation

equipment

. pets, edible crops, or sources

of electrici ty.

Do not make applications to

structures while occupied by

livestock, poultry or

companion animals.

Do not apply to milk rooms

or feed rooms.

Do not make applications to

any animal feedstuffs, water,

or watering equipment.

Do not contaminate any

anim al food, feed, or water

in and around livestock,

poultry, or companion

animal housing when making

applications.



### Each DuPont end

- use product label cont ai ns similar crop rotation instructions, as follows:

Crops on this label and the following crops or crop groups may be planted immediately following the last

application of this product:

Brassica Leafy Vegetables (Crop Group 5);

Bulb Vegetables (Crop Group 3

- 07);

Bushberries (Berry

and Fruit Crop Group subgroup 13

- 07B);

Cereal Gr

ai ns (Crop Group 15 );

Citrus (Crop Group 10

- 10);

Cotton;

Cucurbit Vegetables (Crop Group 9);

Fruiting Vegetables (Crop Group 8

- 10);

Leafy Vegetables (except Brassica s ) (Crop Group 4);

Legume Vegetables (succulent or dried) (Crop Group 6 );

Low Growing Berries (Berry and Fruit Crop Group subgroup 13

- 07G);

Oilseeds (Crop Group 20);

Pome Fruits (Crop Group 11

- 10);

Tuberous and Corm Vegetables (Root and Tuber Vegetables Crop Group subgroup 1C) ;

Stone Fruits (Crop Group12);

Tree Nuts (Crop Gr oup 14).

The following crops or crop groups may be planted 30 days following the last application of the product:

Foliage of Legume Vegetables (Crop Group 7);

Forage, Fodder and Straw of Cereal Gr ai ns (Crop Group 16);

Grass Forage, Fodder and Hay (Crop Group 17);Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 21 of 193 Nongrass Animal Feeds (forage, fodder, straw and hay) (Crop Group 18);

Peanuts;

Leaves of Root and Tuber Vegetables (Crop Group 2);

Root Vegetables (Root and Tuber Vegetables Crop Group subgroup 1A).

All other crops cannot be planted until 1 2 months after the last application of the product. ’



### As cereal gr ai ns and forage, fodder, hay, and straw of cereal gr ai ns are all part of the same cereal

crop, there cannot be separate PBIs for the gr ai ns and the livestock commodities (forage, fodder,

hay, and straw of cereal grains) . Cereal gr ai ns (crop group 15) are subject to the 30 day PBI and

may not be planted immediately following the last application of cyantraniliprole. Similar

considerations apply to Legume Vegetables (succ ulent or dried) (Crop Group 6) and Tuberous

and Corm Vegetables (Root and Tuber Vegetables Crop Group subgroup 1 A ), where 30 day

PBIs may be used

.

Conclusions

. The use directions are adequate to allow evaluation of the residue data relative to

the proposed use s in the U.S. The proposed maximum seasonal rate and minimum RTIs are

supported by the submitted field trial data. The proposed minimum PHIs are supported by the

submitted field trial data

. The proposed plantback intervals are supported by t he rotational crop

field trial data.

The rotational crop restrictions must be amended, as follows:

(1) Cereal grains (crop group 15) may not be planted immediately after use of

cyantraniliprole. Cereal grain (crop group 15) must have a 30 day plantback int erval

(PBI). This is to be consistent with the 30 day PBI for crop group 16 (forage, fodder,

and straw of cereal grains).

(2) Legume vegetables (crop group 6) may not be planted immediately after use of

cyantraniliprole. Legume vegetables (crop group 6) must have a 30 day PBI. This is

to be consistent with the 30 day PBI for crop group7 (foliage of legume vegetables).

(3) Tuberous and corm vegetables (subgroup 1C) may not be planted immediately after

use of cyantraniliprole. The subgroup may be expanded to the e ntire tuberous and

corm vegetables group with a PBI of 30 days. Although a primary crop use exists for

subgroup 1C for the roots, the PBI of 30 days is needed to cover the foliage (under

crop group 2). For example, sweet potato is a member of crop group a 1C, and sweet

potato leaves are in crop group 2.

Many of the field trials with foliar application were conducted with an OD formulation, but the

end

- use product labels are for an SE formulation. Both are dispersions or suspensions in oil to

be diluted in water prior to application. Therefore, the formulations are considered equivalent,

and bridging trials will not be needed. Side

- by

- side trials show similar residues for the two

formulations when applied at the same rate, for example, Brassica flowerhe ad vegetables and

leaf and head lettuce

.

860.1300 Nature of the Residue

- Plants

Monograph for Cyantraniliprole

, Sections B.7.1.1 through B.7.1.4 ; B.7.1.6 (MRIDs 4 8119988

- 48119990

, 48122525 )Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 22 of 193 The petitioner submitted studies pert ai ning to the metabolism of cyantraniliprole in cotton,

lettuce, tomato, and rice

. Two different radiolabels were used in the plant studies , either in the

cyano group [CN] or in the carbonyl next to the pyrazole ring [PC]. T he label positions are

presented below:

1. [CN

14 C]

- cyantraniliprole

2. [PC

14 C]

- cyantraniliprole



### Numerous metabolites were identified in these studies. The chemical structures and report

names for these metabolites are provided in Appendix I.

Cotton

MRID 4 8119988 : Three 150 g ai /ha appl ications were made to cotton via foliar or soil drench

application. Foliar applications were made to plants 3 weeks post

- emergence and at 7 and 14

days after the initial application (BBCH 16, 18 and 19). Soil drench applications were made 7

weeks post

- emer gence and at 7 and 14 days after the first application (BBCH 19 to 51).

TRRs after soil drench treatment were 0.01 ppm or less in all immature leaf samples, <0.01 ppm

in lint and seed, and 0.02

- 0.10 ppm in cotton gin byproducts. TRRs after foliar treatmen ts ranged

from 2.71

- 14.53 ppm at day 0 after the first treatment and 0.42 ppm in leaves 13 days after the

last treatment, and 0.13 ppm in gin byproducts, 0.01 ppm in lint and <0.01 ppm in seed at

harvest (125 days after the last treatment).

Cyantranilipr ole was the major residue in cotton leaves and gin byproducts. Additional

metabolites include: IN

- DBC80, IN

- J9Z38, IN

- JCZ38, IN

- JSE76, IN

- K5A77, IN

- K7H19, IN

MLA84, IN

- MYX98, IN

- N7B69, IN

- NXX70, and IN

- QKV54.

Table 5. Distribution of residues in leaves and cotton gin by

- products following three foliar applications of [CN/ PC

14 C]

- cyantraniliprole

0 DAT 1 a 7 DAT 1 7 DAT 2 7 DAT 3 13 DAT 3 124 DAT 3 Gin by

products

TRR, mg/kg 2.709 5.414 2.654 0.505 0.425 0.131

mg/kg (% TRR)

Extracted residues 86.8% 100% 96.7% 97.8% 97.9% 83.8%Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 23 of 193 Table 5. Distribution of residues in leaves and cotton gin by

- products following three foliar applications of [CN/ PC

14 C]

- cyantraniliprole

0 DAT 1 a 7 DAT 1 7 DAT 2 7 DAT 3 13 DAT 3 124 DAT 3 Gin by

products

Unextracted residues 13.2% <0.1% 3.4% 2.2% 2.0% 16.2%

Extracted residues after

processing and treatment with

acid, alkali and enzymes 2.338

(86.3%) 5.231

(96.6%) 2.399

(90.4%) 0.454

(89.9%) 0.429

(100.9%) 0.103

(79 .0 %)

Loss during processing 0.014

(0.5%) 0.179

(3.3%) 0.165

(6.2%) 0.040

(7.9%) - 0.012

(

- 2.9%) 0.006

(4.8%)

Cyantraniliprole 1.889

(69.7%) 1.066

(19.7%) 1.873

(70.7%) 0.187

(37.3%) 0.115

(27.1%) 0.043

(34.4%)

IN

- DBC80 ND b 0.030

(0.6%) ND ND ND ND

IN

- J9Z38 0.028

(1.0%) 0.190

(3.5%) 0.078

(2.9%) 0.011

(2.3%) 0.006

(1.5%) 0.008

(5.7%)

IN

- JCZ38 ND 0.069

(1.3%) ND ND ND 0.007

(6.1%)

IN

- JSE76 ND 0.039

(0.7%) 0.003

(0.1%) ND ND ND

IN

- K5A77 ND 0.050

(0.9%) ND ND ND ND

IN

- K7H19 ND 0.089

(1.7%) 0.018

(0.7%) 0.019

(4.1%) 0.021

(4.9%) ND

IN

- MLA84 0.091

(3.3%) c 0.049

(0.8%) 0.013

(0.5%) c 0.017

(3.5%) 0.014

(3.3%) c ND c

IN

- MYX98 ND 0.106

(2.0%) ND ND ND ND

IN

- N7B69 ND 0.017

(0.3%) ND ND 0.005

(1.1%) 0.001

(1.2%)

IN

- NXX70

-- 1.172

(21.7%) -- 0.006

(1.1%) -- --

IN

- QKV54 ND 0.557

(10.3%) 0.039

(1.5%) 0.025

(5.0%) 0.016

(3.8%) ND

Unidentified 0.120

(4.4%) d 0.749

(13.9%) e 0.167

(6.9%) f 0.122

(25.2%) g 0.176

(41.3%) h 0.002

(7.3%) i

Total Characterized/Identified 2.12 0

(78.4%) 4.312

(78.2%) 2.211

(83.3%) 0.399

(79%) 0.352

(82.9%) 0.072

(54.7%)

Uncharacterized 0.214

(7.9%) 0.996

(18.4%) 0.188

(7.1%) 0.055

(10.9%) 0.076

(18 .0 %) 0.032

(24.3%)

a: DAT 1 = days after treatment 1 ; DAT 2 = days after treatment 2; DAT 3 = days after treatment 3

b: ND = Not detected

c: Corresponds to a mixture of IN

- MLA84 and IN

- NXX70 (which co

- eluted in the HPLC system)

d: 8 components; none greater than 0.035 mg/kg (1.3%TRR)

e : 47 components; none greater than 0.163 mg/kg (3.0%TRR)

f : 28 components; none greater than 0.047 mg/kg (1. 8%TRR)

g : 32 components; none greater than 0.031 mg/kg (6.2%TRR)

h : 31 components; none greater than 0.042 mg/kg (9.9%TRR)

i : 20 components; none greater than 0.001 mg/kg (0.9%TRR)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 24 of 193 Figure 1 : Proposed Metabolic Pathway of Cyantraniliprole on Cotton

Lettuce

MRID 48119990 : Three 150 g ai /ha applications were made to lettuce via foliar or soil drench

application. Foliar applications were made to plants 3 weeks post

- emergence (BBCH 14/15

equivalent to BBCH 33 for fruit development) and at 7 (BBCH 16 equivalent to BBCH 35) and

14 (BBCH 18 equivalent to BBCH 36) days after the initial application. Soil drench applications

were made 7 weeks post

- emergence (BBCH 18/19 equivalent to BBCH 36) and at 7 (BBCH 19

equivalent to BBCH 37/38) and 14 (BBCH 19 equiv alent to BBCH 37/38) days after the first

application.

TRR following soil drench treatment declined from 0.14 ppm 7 days after the first application to

0.01 ppm at harvest (32 days after the last application). Higher residues were observed

following foli ar application and residues declined from 10.84 ppm initially to 0.03 ppm at

maturity.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 25 of 193 Cyantraniliprole was the major residue in lettuce. Additional metabolites include: IN

- K5A7 9

, IN

- MLA84, IN

- NXXX70, IN

- J9Z38, IN

- DBC80, IN

- N7B69, IN

- K5A79, IN

- JSE76, IN

HGW87 , IN

- K5A77, and IN

- QKV54. IN

- J9Z38 was a major metabolite in matur e lettuce from

foliar treatment.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 26 of 193 Table 6. Distribution of residues in lettuce following three foliar applications of [CN/PC

14 C]

- cyantraniliprole

14 C

- Residue Sampling point

0DAT 1 7DAT 1 0DAT 2 7DAT 2 0DAT 3 7DAT 3 14DAT 3 32DAT 3 Maturity

TRR, mg/kg 10.844 1.669 9.662 2.801 7.789 1.993 0.983 0.032

mg/kg (% TRR)

Extracted residues 99.9% 99.0% 98.6% 98.4% 97.7% 91.7%

Unextracted residues <0.1% 1.1% 1.3% 1.5% 2.3 % 8.3%

Extracted residues after processing

and treatment with acid, alkali and

enzymes 10.79 0

(99.5%) 1.57 0

(94.1%)

Extraction

not

performed 2.812(100.4%)

Extraction

not

performed 1.771

(89 .0 %) 0.936

(95.2%) 0.030

(92.2%)

Loss during processing 0. 054

(0. 5 %) 0.080

(4.8%) - 0.048

(

- 1.7%) 0.189

(9.5%) 0.025

(2.5%) <0.001

(

- 0.5%)

Cyantraniliprole 10.618

(97.9%) 1.320

(79.1%) 2.446

(87.3%) 1.564

(78.5%) 0.716

(72.6%) 0.016

(50.3%)

IN

- DBC80 ND 0.009

(0.5%) 0.041

(1.6%) 0.031

(1.6%) ND ND

IN

- N7B69 ND 0.017

(1.0%) 0.012

(0.4%) ND ND

IN

- K5A79 ND 0.018

(1.2%) ND ND ND ND

IN

- JSE76 ND 0.012

(0.7%) ND 0.014

(0.7%) ND ND

IN

- HGW87 ND 0.010

(0.6%) ND 0.018

(0.9%) ND ND

IN

- K5A77 ND ND 0.036

(1.2%) ND ND ND

IN

- QKV54 ND ND 0.042

(1.4%) 0.017

(0.9%) ND ND

IN

- MLA84/

IN

- NXX70 a 0.034

(0.3%) 0.050

(3.0%) 0.058

(2.0%) 0.027

(1.4%) 0.027

(2.6%) 0.001

(4.9%)

IN

- J9Z38 0.042

(0.4%) ND 0.028

(1.0%) 0.021

(0.8%) 0.023

(2.3%) 0.011

(23.3%)

Unidentified 0.035

(0.3%) b 0.103

(6.6 % ) c 0.117

(4.1%) d 0.078

(3.8%) e 0. 077

( 7 .4%) f < 0.001

( 1.3 %)

Total Characteriz ed/ Identified 10.729

(98.9%) 1.539

(92.7%) 2.780 (99%) 1.770

(88.6%) 0.843

( 84 .9%) 0.028

(79.8%)

Uncharacterized 0.065

(0.6%) 0.023

(1.4%) 0.039

(1.4%) 0.008

(0.4%) 0.101

(10.3%) 0.004

(12.4%)

a: HPLC method 1 did not resolve IN

- MLA84 from IN

- NXX70

b : 7 components, none greater than 0.006 mg/kg (0.1% TRR)

c : 46 components, none greater than 0.009 mg/kg (0.6% TRR)

d: 29 components, none greater than 0.011 mg/kg (0.4% TRR)

e : 27 components, none greater than 0.009 mg/kg (0.4% TRR)

f : 33 components, none greater than 0.007 mg/kg (0.7% TRR)

ND = Not detectedCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 27 of 193 Figure 2 : Proposed Metabolic Pathway of Cyantraniliprole on Lettuce

Tomato

MRID 48119989 : Three 150 g ai /ha applications were made to tomatoes v ia foliar or soil drench

application. Foliar applications were made to plants 3 weeks post

- emergence (BBCH 14

- 15) and

at 7 (BBCH 16) and 14 days (BBCH 53, 61) after the initial application. Soil drench applicationsCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 28 of 193 were made 7 weeks post

- emergence (BBCH 19 , 51) and at 7 (BBCH 51) and 14 days (BBCH 55,

61) after the first application.

TRRs after soil drench treatment were 0.01 ppm in immature leaves 7 and 14 days after the third

application, and <0.01 ppm in leaves and fruit at harvest (125 days after the t hird application.

TRRs after foliar treatments ranged from 2.55

- 8.50 ppm at day 0 after the first treatment and

declined to 1.30 ppm 14 days after the last application. Residues at harvest were <0.01 ppm in

leaves and fruit (125 days after the last treatme nt).

Cyantraniliprole was the major residue in tomato leaves. Additional metabolites include: IN

K7H19, IN

- JCZ38, IN

- DBC80, IN

- N7B69, IN

- MYX98, IN

- JSE76, IN

- HGW87, IN

- QKV54,

IN

- MLA84, IN

- NXX70 and IN

- J9Z38.

Table 7. Distribution of residues in tomato leaves following three foliar applications of [CN/PC

14 C]

- cyantraniliprole

14 C

- Residue Sample point

0DAT 1 7DAT 1 7DAT 2 7DAT 3 14DAT 3 TRR, mg/kg 2.546 1.849 4.806 2.216 1.298

Extracted residues 99.0% 99.8% 100.0% 99.2% 97.7%

Unextracted residues 1 .0 % 0.1% 0.1% 0.8% 2.3%

Extracted residues after

processing and treatment with

acid, alkali and enzymes 2.683

(105.4%) 1.951

(105.5%) 4.878

(101.5%) 2.411

(108.8%) 1.328

(102.3%)

Loss during processing

- 0.163

(

- 6.4%) - 0.104

(

- 5.6%) - 0.077

(

- 1.6%) - 0.213

(

- 9.6%) - 0.059

(

- 4.6%)

Cyantraniliprole 2.429

(95.3%) 1.132

(61.1%) 4.154

(86.5%) 1.377

(62.2%) 0.562

(43.4%)

IN

- K7H19 ND ND ND 0.025

(1.2%) 0.019

(1.4%)

IN

- JCZ38 ND 0.019

(1.0%) 0.010

(0.2%) 0.022

(1.0%) 0.036

(2.8%)

IN

- DBC80 ND 0.033

(1.9%) 0.021

(0.5%) 0.034

(1.5%) ND

IN

- N7B69 ND ND 0.016

(0.3%) 0.019

(0.8%) 0.037

(2.9%)

IN

- MYX98 ND ND ND 0.023

(1.0%) 0.018

(1.5%)

IN

- JSE76 ND ND ND ND 0.012

(1.0%)

IN

- HGW87 ND ND 0.028

(0.6%) ND ND

IN

- QKV54 ND ND 0.040

(0.8%) 0.062

(2.8%) 0.054

(4.3%)

IN

- MLA84/

IN

- NXX70 0.023

(0.9%) 0.213

(11.5%) 0.149

(3.1%) 0.147

(6.6%) 0.074

(5.8%)

IN

- J9Z38 0.022

(0.9%) 0.029

(1.6%) 0.051

(1.2%) 0.114

(5.1%) 0.055

(4.4%)

Unidentified 0.102

(5.0%) a 0.197

(10.7%) b 0.137

(2.6%) c 0.352

(15.9%) d 0.265

(22.2%) e

Total Characterized 2.599

(102.1%) 1.623

(87.8%) 4.605

(95.8%) 2.174

(98.1%) 1.164

(89.7%)

Uncharacterized 0.084

(3.3%) 0.327

(17.7%) 0.273

(5.7%) 0.237

(10.7%) 0.164

(12.6%)

a: 32 components, none greater than 0. 009 mg/kg (0.4%TRR)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 29 of 193 b: 33 components, none greater than 0.025 mg/kg (1.3%TRR)

c: 29 components, none greater than 0.019 mg/kg (0.4%TRR)

d: 51 components, none greater than 0.069 mg/kg (3.1%TRR)

e: 45 components, none greater than 0.072 mg/kg (5.6%TRR)

ND = Not detected



### Figure 3 : Proposed Metabolic Pathway for Cyantraniliprole on Tomato

Rice

MRID 481 22525 : Rice was grown in a glasshouse under flooded conditions. Three 150 g ai /ha

applications were made to rice via foliar spray at the 3

- 4 leaf stage and at 7 and 14 days after the

initial application (BBCH 13

- 14 at each application). One 300 g ai /ha application was made to

rice via soil application at the 3

- 4 leaf stage (BBCH 13).

Following foliar applications, TRR ranged from 0.383

- 2.13 ppm in foliage when sampled w ithin

14 days of first 3 treatments, 0.446 ppm in straw, 0.024 in gr ai n and 0.447 ppm in roots atCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 30 of 193 harvest (140 days after the last application). Following one soil application, TRR ranged from

0.40

- 0.42 ppm 56 days after treatment, 0.278

- 0.297 ppm in straw , 0.012

- 0.029 ppm in gr ai n, and

0.282

- 0.367 ppm in roots at harvest (175 days after treatment).

Cyantraniliprole was the major residue in rice foliage, straw, and gr ai n. Additional minor

metabolites include: IN

- JCZ38, IN

- JSE76, IN

- K7H19, IN

- MLA84, IN

- J9Z3 8, IN

- DBC80, IN

N7B69, IN

- K5A77, and IN

- K5A78.

Table 8 : Distribution of residues in rice following three foliar applications with [CN/PC

14 C]

- cyantraniliprole

Tissue Foliage Straw a Gr ai n a

Days after foliar treatment (DAT) 0 DAT 1 7 DAT 1 7 DAT 2 7 DAT 3 14 DAT 3 140

DAT 3 140

DAT 3

TRR, mg/kg 2

. 126 0

. 383 1 .000 1 .560 1

. 207 0

. 446 0

. 024

Extracted residues 99.9 92.1 99.0 96.6 95.3 83.7 48.7

Unextracted residues 0.002

(0.1%) 0.03 0

(7.8%) 0.01 0

(1 .0 %) 0.053

(3.4%) 0.057

(4.7%) 0.073

(16.3%) e 0.012

(51.3%)

Extracted residues after processing and

treatment with acid, alkali and enzymes 2.152

(101.2%) 0.357

(93.2%) 1.030

(103 .0 %) 1.445

(92.6 % ) 1.168

(96.8%) 0.437

(98 .0 %) e 0.010

(42.9%)

Loss during processing - 0.028

(

- 1.3%) - 0.004

(

- 1 .0 %) - 0.040

(

- 4 .0 %) 0.062

(4 .0 %) - 0.018

(

- 1.5 % ) - 0.016

(

- 3.5%) 0.001

(5.8%)

Cyantraniliprole 2.127

(100 .0 %) 0.347

(90.7%) 0.955

(95.5 % ) 1.179

(75.6%) 0.979

(81.1%) 0.109

(24.4%) 0.005

(20.9%)

IN

- JCZ38 ND ND ND 0.009

(0.6%) 0.009

(0.8%) 0.042

(9.4%) ND

IN

- JSE76 ND ND ND ND 0.002

(0.2%) 0.040

(9 .0 %) ND

IN

- K7H19 ND ND ND ND ND 0.011

(2.4%) ND

IN

- MLA84 ND ND 0.006

(0.6%) 0.024

(1.5%) 0.023

(1.9%) ND ND

IN

- J9Z38 0.014

(0.6%) 0.010

(2.5%) 0.063

(6.3%) 0.112

(7.2%) 0.131

(10.9%) 0.018

(4 .0 %) < 0.001

(1.5%)

IN

- DBC80 ND ND ND ND ND 0.014

(3.2%) ND

IN

- N7B69 ND ND ND 0.027

(1.7%) ND ND 0.001

(2.2%)

IN

- K5A77 0.012

(0.6%) ND ND ND 0.010

(0.8%) 0.024

(5.3%) ND

IN

- K5A78 ND ND ND 0.012

(0.8%) 0.005

(0.2%) ND 0.001

(2.6%)

Unidentified ND ND 0.005

(0.5%) 0.084 b

(5.3%) b 0.011

(0.9%) 0.131 c

(29.4%) c 0.003 d

(15.7%) d

Total identified and characterized 2.152

(101.2%) 0.357

(93.2%) 1.029

(102.9%) 1.446

(92.7%) 1.168

(96.8%) 0.388

(87.1%) 0.010

(42.9%)

Uncharacterized <0.001 <0.001 0.001 - 0.002

(

- 0.1%) <0.001 0.049

(10.9%) <0.001

a: Final harvest

b: 8 components, none greater than 0.035 mg/kg (2.3%)

c: 7 components, none greater than 0.036 mg/kg (8.1%)

d: 17 components, none greater than 0.002 mg/kg (8%)

e: 10.9% of the TRR was further extracted with enzymes (1.2%) , acid (3.2%) and alkali treatment (8.4%)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 31 of 193 Figure 4 : Proposed Metabolic Pathway for Cyantraniliprole on Rice

Conclusions

. The submitted plant metabolism studies are adequ ate

. The results of the submitted

studies with cotton, lettuce, tomato and rice indicate that the metabolism of cyantraniliprole is

similar in these crops. Overall, total radioactive residues were greater following foliar treatment.

Cyantraniliprole was the principal residue in most crop fractions at various sampling points up to

cr op maturity. Numerous metabolites were detected in crop matrices. The metabolism of

cyantraniliprole in plants appears to proceed via loss of water leading to the formation of

quinazolinone ring, oxidation of the cyano group, and hydroxylation of the N

- met hyl and/or tolyl

methyl group.

The submitted studies are adequate to satisfy plant metabolism data requirements. It should be

pointed out that use of a mixture of two radiolabeled substances for foliar applications as a single

formulation is acceptable s ince cleaved metabolites such as IN

- DBC80 and IN

- QKV54 wereCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 32 of 193 found in low concentrations in some plant studies. Even after doubling the concentration of IN

DBC80 to account for the mixed label, the change in concentration is insignificant. IN

- QKV54

was a lo w level metabolite found primarily in immature plant samples and thus considered to be

in significant as a metabolite. It is also noted that the samples collected at harvest from the

metabolism studies were substantially longer (125 days after last applicat ion) than the proposed

treatment

- to

- harvest intervals (7 days or less). A differen t metabolic pathway on fruits and gr ai n

could not be excluded. However, immature and mature samples of four different crop groups

covering a wide range of agro

- climatic condi tions were taken that showed very similar metabolic

pathways. The formation of a new metabolite soon after application would be very un likely , and

even so, it would be very low.

The residue of concern in plant commodities for tolerance enforcement and risk assessment is

cyantraniliprol e (ROCKS decisio n memo, D404411, 9/25 /2012 )

.

860.1300 Nature of the Residue

- Livestock

Monograph for Cyantraniliprole

, Sections B.7.2.1 and B.7.2. 2 , B.7.2.5 (MRIDs 48119991

- 48119992 )



### There are several livestock fe edstuffs associated wit h the proposed uses of cyantraniliprole . The

petitioner provided livestock metabolism studies with laying hens and lactating goats , which are

summarized below.

Hen

MRID 4 8119992 : In a metabolism study with [ CN

14 

### C ]

- or [PC

14 

### C]

- cyantraniliprole

, 5 hens

per radiolabel and 2 concurrent controls were orally dosed for 14 consecutive days in 24 h

intervals with 1 .0 7 mg per kg body weight per day (corresponding to 12.8

- 2 1.3 mg ai /kg feed or

10 ppm in the diet) and sacrificed about 2 3 hours after the last dose. The dosing level

corresponds to

~ 300 x the estimated dietary burden of cyantraniliprole to poultry (see Table 8 ).

Total radioactivity was measured in the excreta and eggs , which were collected once or twice

d ai ly, as well as i n the dissected tissues muscle, fat, liver

, and skin collected at sacrifice. The

excreta, eggs (white, yolk) and liver were analyzed for parent compound and metabolites.

The overall recovery (sum of radioactivity in the excreta, eggs as well as organs a nd tissues) was

101

- 103 % of the administered dose. The majority of the radioactivity ( 9 7 .0

- 99.7 % of the total

dose) was detected in the excreta collected before sacrifice. Radioactivity in e ggs accounted for

0.61

- 0.47 % of the total dose

. At sacrifice

, the residues in the edible organs and tissues dissected

from the bodies amounted to 0.66

- 0.54 % of the total dose. From this, the bulk of the residue was

detected in e gg white

.

For analysis of parent compound an d metabolites, Day 1

- 14 egg white and egg yolk , liver, and

Day 1

- 14 excreta samples from all animals were respectively pooled

, and the pooled samples

were extracted with 9:1 v/v CAN (acetonitrile) : water. T he resulting extracts of eggs, liver, and

excreta were combined and the radio active content was determined by LSC of duplicate aliquots

. The post extracted solids (PES) of liver samples were further treated with protease in pH 7 buffer

at 37C for 24 hours before LSC analysis. Extracts were analyzed using reversed phase HPLC

equippe d with an UV detector at 254 nm. Identification of residues was by comparison of HPLCCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 33 of 193 retentions times with those of authentic reference standards. Confirmation of metabolites was by

LC/MS/MS.

The major component in egg white and egg yolk was cyantranilip role , at 32.5

- 41.7 % and 10.3

-

9.33% TRR , respectively; no parent was detected in liver. Other metabolites identified in eggs

were IN

- HGW87, IN

- J9Z38, IN

- MLA84, IN

- MYX98, IN

- NBC94, IN

- JSE76, IN

- K5A77, and

IN

- K5A79; in liver were IN

- J9Z38, IN

- MLA84, IN

- MYX98 , IN

- NBC94, IN

- K5A78, IN

K5A79, and IN

- N7B69. In eggs, IN

- HGW87, IN

- J9Z38, and IN

- MLA84 were present at >10%.

In liver, all the metabolites were present at <0.01 ppm. Unchanged cyantraniliprole was the

major residue (68

- 77%) found in excreta.

Table 9. Distribution of residues (mg/kg parent equivalents and % TRR) in eggs and liver from hens dosed with

[CN

14 C]

- cyantraniliprole

Residue Egg white

(Day 1

- 14

Composite) Egg yolk

(Day 1

- 14

Composite) Liver

Aqueous ACN

extract Protease digest

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

Total 100.0 0.259 100.0 0.092 100.0 0.137 100.0 0.137

Total extracted 99.01 0.256 79.30 0.073 17.07 0.023 38.09 0.052

Cyantraniliprole 32.48 0.084 10.28 0.009 ND ND

IN

- HGW87 0.61 0.002 11.98 0.011 ND ND

IN

- J9Z38 29.21 0.075 7.42 0.006 2.08 0.003 ND

IN

- MLA84 18.66 0.049 11.56 0.011 0.89 0.001 ND

IN

- MYX98 6.40 0.017 5.42 0.006 0.42 0.001 1.10 0.002

IN

- NBC94 0.96 0.002 0.86 0.001 ND 0.40 0.001

Total characterized unknowns a 7.75 0.021 23.62 0.025 7.58 0.010 22.33 0.042

Total characterized/

identified b 96.07 0.250 71.14 0.068 10.97 0.015 23.82 0.045

Total uncharacterized/

unidentified c 2.94 0.008 8.16 0.005 6.10 0.008 16.94 0.023

Total unextracted 0.99 0.003 20.70 0.019 82.93 0.114 44.97 0.062

a : Consists of HPLC peaks that do not match the retention time of any known reference standard

b : Total characterized/identified = Sum of identified metabolites + Total characterized unknowns

c : Total uncharacterized/unidentified = Total extracted

– Total c haracterized/identified

ND = Not Detected

Table 10. Concentration of residues (mg/kg parent equivalents and % TRR) in, eggs and liver from hens dosed with

[PC

14 C]

- cyantraniliprole

Residue Egg white

Day 1

- 14

composite Egg yolk

Day 1

- 14

composite Liver

% TRR mg/kg % TRR mg/kg Aqueous ACN

extract Protease digest

% TRR mg/kg %

TRR mg/kg

Total 100.0 0.203 100.0 0.087 100.0 0.174 100.0 0.174

Total extracted 99.7 0.202 83.1 0.072 23.0 0.040 37.6 0.065

Cyantraniliprole 41.7 0.085 9.33 0.008 ND ND

IN

- HGW87 0.74 0.001 ND ND ND

IN

- J9Z38 17.1 0.034 13.1 0.011 ND ND

IN

- JSE76 ND 1.90 0.002 ND ND

IN

- JCZ38 ND ND ND 0.23 <0.001

IN

- K5A77 ND 6.19 0.005 ND ND

IN

- K5A78 ND ND 0.23 <0.001 NDCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 34 of 193 IN

- K5A79 3.90 0.007 1.52 0.001 0.27 <0.001 0.45 0.001

IN

- K7H19 ND ND ND 0.46 <0.001

IN

- MLA84 18.2 0.037 16.8 0.015 3.98 0.007 ND

IN

- MYX98 8.13 0.016 2.09 0.002 0.27 <0.001 ND

IN

- N7B69 ND ND ND 0.55 0.001

IN

- NBC94 2.86 0.005 1.24 0.001 ND ND

Total characterized

unknowns a 0.98 0.002 17.4 0.015 10.5 0.020 25.0 0.045

Total characterized/

identified b 96.2 93.6 0.187 69.6 0.060 15.2 0.027 26.7 0.047

Total

uncharacterized

/unidentified c 0.00 6.09 0.014 13.5 0.012 7.8 0.013 10.9 0.018

Total unextracted 3.84 0.33 0.001 16.9 0.015 77.0 0.134 39.5 0.053

a : Consists of HPLC peaks that do not match the retention time of any known reference standard

b : Total characterized/Identified = Sum of identified metabolites + Total characterized unknowns

c : Total uncharacterized/unidentified = Total extracted

– Total characterized /identified

ND = Not Detected



### Figure 5: Proposed Metabolic Pathway for Cyantraniliprole in Egg

- Laying HensCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 35 of 193 Conclusions

- Hen

. The submitted hen metabolism studies are adequate. The majority (97

- 100%) of the dose was excreted. Unchanged parent was th e major residue in excreta. Transfer of

residues to eggs, fat, muscle, and liver was low. Residues in fat and muscle were <0.01 ppm.

Unchanged cyantraniliprole was the major residue in egg white and egg yolk. Other residues

found in eggs include IN

- J9Z38 a nd IN

- MLA84. Parent was not detected in the liver and a

number of metabolites formed instead. These metabolites resulted from cyclization, hydrolysis of

the cyano group, hydroxylation of the benzilic and N

- methyl carbons, and cleavage at the

pyrazole carb onyl.

Goat

MRID 4 8119991 : A goat was orally dosed for 7 consecutive days, at 24 h intervals, with 0.44

mg [ CN

14 

### C]

- or [PC

14 

### C ]

- cyantraniliprole per kg body weight per day (corresponding to ~35

mg ai /kg feed or 10 ppm in the diet ) and sacrificed at about 24 hours after the last dose. The

dosing level corresponds to ~ 2 4 x t he estimated dietary burden of cyantraniliprole to beef cattle

and d ai ry cattle (see Table 8 ). Total radioactivity was measured in feces , urine, bile, milk, live r,

kidney, muscle, and fat (omental, renal, subcutaneous). The milk

, liver, kidney, muscle and fat

were analyzed for parent compound and metabolites.

The overall recovery (sum of radioactivity in the excreta, milk as well as organs and tissues) was

96

- 97 % of the total administered dose. As percentages of the total administered dose,

radioactive residues were 84

- 88% in feces , 6.7

- 6.9% in urine, and 1.4

- 2.3% in cage wash.

In tissues, TRR were highest in liver , at 0.46

- 0.495 ppm

. The TRR for the other or gans and

tissues, were 0.117

- 0.177 ppm in kidney, 0.080

- 0.147 ppm in milk, 0.045

- 0. 114 ppm in fat, and

0.020

- 0.043 ppm in muscle. In total, the residues in all tissue samples were estim ated to be

about 2.0 % of the total dose.

The major component in all the edible matrices was cyantraniliprole ( 17 .1% to 49 .6 % TRR).

Other metabolite s identified at >10% TRR were IN

- N7B69, IN

- MYX98 , and IN

- J9Z38 (fat)

.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 36 of 193 Table 11. : Distribution of radioactive residues in goat milk and tissues following administration of [CN

14 C]

- cyantraniliprole

Component Milk a Liver Kidney Muscle Fat b

%TRR mg/kg Solvent Extract Protease Digest %TRR mg/kg %TRR mg/kg %TRR mg/kg %TRR mg/kg % TRR mg/kg

TRR 100.0 0.076 100.0 0.430 100.0 0.430 100.0 0.136 100. 0.027 100. 0.052

Extracted 99.3 0.075 54.3 0.233 26.8 0.115 62.7 0.085 61.0 0.016 90.4 0.047

Cyantraniliprole 39.5 0.030 17.1 0.073 0.3 0.002 12.7 0.017 30.3 0.009 30.8 0.016

IN

- K7H19 2.6 0.001 1.0 0.004 0.9 0.005 3.0 0.004 4.6 0.001 1.9 <0.001

IN

- JCZ38 7.2 0.005 0.83 0.004 0.4 0.003 ND ND ND

IN

- N7B69 11.8 0.008 0.64 0.002 1.8 0.008 ND ND 1.9 <0.001

IN

- K5A79 ND 0.61 0.003 ND ND ND 1.9 <0.001

IN

- MYX98 15.1 0.011 2.5 0.011 0.1 <0.001 7.1 0.010 ND 1.9 <0.001

IN

- JSE76 ND ND ND ND ND ND

IN

- HGW87 1.1 <0.001 1.1 0.004 0.1 0.001 0.61 0.001 ND ND

IN

- K5A77 ND 5.4 0.023 ND ND ND ND

IN

- NBC94 ND ND ND ND ND 1.9 <0.001

IN

- K5A78 ND 0.83 0.003 0.2 0.001 ND ND 1.9 <0.001

IN

- MLA84 ND 3.7 0.017 0.2 0.001 4.1 0.005 ND 1.9 0.001

IN

- J9Z38 0.48 <0.001 ND ND 1.1 0.001 ND 26.9 0.014

Unknowns 12.7 0.006 13.2 c 0.053 c 18.6 ed 0.080 d 13.2 0.018 7.6 0.003 11.5 0.006

Total Char./ Ident. 90.5 0.061 46.9 0.202 22.6 0.101 41.8 0.056 42.5 0.013 75.0 0.039

Total Unextracted 0.7 0.001 45.7 0.197 18.9 e 0.082 e 37.4 0.051 39.2 0.011 9.6 0.005

a : Day 1

- 7 Composite Sample

b : Concentrations are a mean of omental, renal, and subcutaneous fat samples analyzed.

d : 13 components, none greater than 0.006 mg/kg (1.4%TRR)

e : 32 components, none greater than 0.006 mg/kg (1.5%TRR)

f : Terminal unextracted residues following protease digestion

ND = Not Detected.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 37 of 193 Table 12. : Distribution of radioactive residues in goat milk and tissues following administration of [PC

14 C]

- cyantraniliprole

Component Milk a Liver Kidney Muscle Fat b

%

TRR mg/kg Solvent Extract Protease Digest %

TRR mg/kg %

TRR mg/kg %

TRR mg/kg % TRR mg/kg % TRR mg/kg

TRR 100. 0.140 100.0 0.500 100.0 0.500 100.0 0.206 100.0 0.040 100.0 0.119

Extracted 99.0 0.139 59.5 0.296 20.7 0.103 78.6 0.162 80.9 0.032 98.3 0.117

Cyantraniliprole 49.6 0.070 27.3 0.136 ND 18.9 0.040 15.3 0.006 45.4 0.054

IN

- K7H19 0.57 0.001 0.95 0.005 0.5 0.003 2.3 0.004 ND <0.8 <0.001

IN

- JCZ38 1.32 0.002 0.42 0.002 ND 0.64 0.001 ND ND

IN

- DBC80 ND ND ND ND ND <0.8 <0.001

IN

- N7B69 2.01 0.003 0.32 0.002 ND 1.7 0.003 ND <0.8 <0.001

IN

- K5A79 ND 0.61 0.003 ND ND ND 2.5 <0.003

IN

- MYX98 18.3 0.026 3.6 0.017 ND 7.1 0.014 32.8 0.014 <0.8 <0.001

IN

- JSE76 ND ND ND ND ND ND

IN

- HGW87 0.69 0.001 1.0 0.005 ND 1.1 0.002 ND ND

IN

- K5A77 ND 5.7 0.028 ND 0.70 0.001 4.4 0.001 <0.8 <0.001

IN

- NBC94 ND ND ND ND ND 1.7 0.002

IN

- K5A78 1.26 0.002 3.4 0.017 0.3 0.002 ND ND <0.8 <0.001

IN

- MLA84 2.24 0.003 5.3 0.026 0.4 0.002 1.8 0.004 ND 1.7 0.002

IN

- J9Z38 3.72 0.005 0.9 0.004 ND ND 1.1 <0.001 24.4 0.029

Unknowns 8.81 0.012 6.2 c 0.031 c 11.6 d 0.059 d 36.9 e 0.076 e 17.1 0.006 5.0 0.006

Total Charac./Ident. 88.4 0.125 55.7 0.279 12.8 0.066 71.0 0.145 70.9 0.027 79.8 0.095

Total Unextracted 0.83 0.001 40.5 0.204 19.9 f 0.101 21.5 0.044 19.2 0.008 1.7 0.002

a Day 1

- 7 Composite Sample

b Concentrations are a mean of omental , renal and subcutaneous fat samples analyzed

c 7 components, none greater than 0.008 mg/kg (1.6%TRR)

d 19 components, none greater than 0.009 mg/kg (1.8%TRR)

e 10 components, none greater than 0.023 mg/kg (11.4%TRR)

f Terminal unextracted residues following protease digestion

ND = Not DetectedCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 38 of 193 Figure 6 : Proposed Metabolic Pathway of Cyantranilirprole in Lactating Goats

Conclusions

- Goat

. The submitted goat metabolism studies are adequate to satisfy data

requirements. Cyantraniliprole residues were rapidly eli minated by the lactating goat primarily

in the excreta (94

- 96%). In feces , unchanged cyantraniliprole was the major residue accounting

for 67

- 71% of the dose. In urine, cyantraniliprole was extensively metabolized and represented

no greater than 0.5% of th e dose. Transfer of residues to edible tissues was low at 1

- 2% of the

dose. Also, about 1

- 2% of the dose was eliminated through the milk. The major residue in milk,

liver, kidney, muscle and fat was cyantraniliprole. About a dozen metabolites were detecte d at

difference levels in different tissues. The formation of these metabolites indicate a variety of

chemical reactions similar to those found in hens, such as cyclization, hydroly sis of the cyano

group, hydroxylation of the benzilic and N

- methyl carbons , and cleavage of the pyrazole

carbonyl.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 39 of 193 Overall Conclusions

- Livestock Metabolism. The nature of the residue in livestock is adequately

understood. The metabolism of cyantraniliprole in poultry and ruminants was found to be

similar. The residue of c oncern for tolerance enforcement is cyantraniliprole , and the residues of

concern for risk assessment include cyantraniliprole and its metabolites IN

- J9Z38, IN

- N7B69,

IN

- MLA84, and IN

- MYX98 (ROCKS decision memo, D404411, 9/25 /2012 )

.

860.1340 Residue Analytical Methods

Monograph for Cyantraniliprole

, Section B.5.2 (MRIDs 48119922, 48119923, 48119924, 48119925, 48119928,

48119929, 48119932, 48119933, 48119934, 48119998, 48119999, 48120001, 48122526, 48122534 )



### Analytical methods are av ai lable for tolerance enforcement in plants and processed commodities,

and livestock commodities.

DuPont

- 18846, m ethod for the analysis of cyantraniliprole and its metabolites (IN

- N7B69, IN

JCZ38, IN

- K7H19, IN

- MYX98, IN

- MLA84 and IN

- J9Z38) in various crops with LC/M S/MS

detection , was validated in grapes, apples, peaches, tomatoes, almonds (nut meal), lettuce, whe at

gr ai n, wheat straw, potatoes, lemons, rape seed , tomato paste, and sun

- dried tomato with an LOQ

of 0.01 ppm cyantraniliprole

.

Briefly, A 10 g crop sample is extracted twice with 20 mL of a 90:10 mixture of

acetonitrile:water solution by shaking at high speed on a Geno Grinder Shaker. Ball bearings are

present during the extraction to aid the effectively extracting cyantraniliprole and metabolites IN

J9Z38, IN

- JCZ38, IN

- K5A79, IN

- K7H19, IN

- MLA84, IN

- MYX98, and IN

- N7B69 from the

plant material. The samples were shaken at 700 cycles per minute on a Geno Grinder Shaker for

2 minutes. The extract was then centrifuged at 3000 RPM for 5 minutes. Next, the su pernatant

was decanted into a clean 50

- mL centrifuge tube. An additional 20 mL of acetonitrile:water

extraction solution was added to the sample pellet, shaken at 700 cycles per minute on a Geno

Grinder Shaker for 2 minutes, then the extract was centrifuge d at 3000 RPM for 5 minutes. The

supernatant was combined into the same 50

- mL centrifuge tube used previously in the process.

The extract was diluted to 50 mL using acetonitrile:water extraction solution and vigorously mix

the extract. Approximately 1 mL o f extract was filtered with a 3

- mL syringe and a 0.2

- μm PTFE

filter into a 13

- mL centrifuge tube. Then, 0.10 mL of the filtered extract was pipetted into a

HPLC vial and an additional 0.40 mL of methanol and 0.50 mL of de

- ionized water solution

were pipett ed and mixed well. Samples were then analyzed using LC

- MS/MS (ESI MRM

positive mode). IN

- MYX98 was also quantified in wheat using the negative mode.

The following mass transitions are used for qualitative identification and quantification.

Analyte Mass t ransition 1 Mass transition 2

Cyantraniliprole 475 → 444 475 → 286

IN

- J9Z38 457 → 188 457 → 299

IN

- JCZ38 493 → 286 493 → 462Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 40 of 193 INK7H19 479 → 286 479 → 462

IN

- MLA84 443 → 298 443 → 407

IN

- MYX98 491 → 444

489 → 379 (negative mode) 491 → 473



### Recoveries for all matrices except tomato paste and sun

- dried tomato ranged from 7 8

- 1 03 % , and

from 77

- 94% for tomato paste and sun

- dried tomato when fortified at 0.01 ppm cyantraniliprole

. DuPont

- 25543

, analytical method for the determination of cyantraniliprole , IN

- HGW87, IN

N7B69, IN

- K7H19, IN

- JCZ38, IN

- MYX98, IN

- J9Z38 and IN

- MLA84 in bovine tissues, milk

and eggs using LC/MS/MS

, was validated with an LOQ of 0.01 ppm cyantraniliprole in muscle,

liver, kidney, fat, milk, and egg.

Briefly, a 10 g sample is extracted twice with 20 to 25 mL of acetonitrile using a Geno

- Grinder

for 2 minutes. The two extractions are combined and brought to a volume of 50 mL using

acetonitrile. All of the extracts were purified using the same extraction procedure. Using the 10

-

mL aliquot previously extracted, 3 mL of hexane was added and shaken by hand for 1 minute.

The sample was then centrifuged on the bench top centrifuge for 5 minutes. The sample was

removed from the centrifuge and the upper layer of hexane removed and discarded. A sa mple

was taken and put on a nitrogen evaporator until the sample was approximately 0.5 mL. The

sample was then increased to a volume of 5 mL by adding methanol and then vortexed and

sonicated. Then, 5 mL of water was added for a 50/50 solution, sonicated, vortexed, and filtered

before analyzing. In cases where matrix interferences is noted, an additional purification step

involving salt out should be included after the hexane partition. This step consists of adding 0.5

grams of salt to the sample, followed by shaking for 1 minute by hand. The sample is then

centrifuged for 10 minutes at 3000 RPM, and the upper layer is transferred to a new 50

- mL

centrifuge tube.

Cyantraniliprole, IN

- HGW87, IN

- J9Z38, IN

- JCZ38, IN

- K5A79, IN

- K7H19, IN

- N7B69, IN

MLA84, and IN

- MYX98 were separated from co

- extracts by reversed phase liquid

chromatography (LC) and detected by mass spectrometry/mass spectrometry (MS/MS) using

positive ion atmospheric pressure chemical ionization (APCI). The transitions used are:

Analyte Mass transition 1 Mass transition 2

Cyantraniliprole 475 → 443 475 → 286

IN

- J9Z38 457 → 298 457 → 188

IN

- JCZ38 493 → 462

INK7H19 479 → 462 479 → 286

IN

- MLA84 443 → 298 443 → 407

IN

- MYX98 491 → 444Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 41 of 193 IN

- N7B69 491 → 286

IN

- K5A79 480 → 463 480 → 286

IN

- HGW87 459 → 284 459 → 112



### Extraction efficiency

F or plant commodities, DuPont submitted data for the enforcement method using samples of

lettuce, beet foliage

, wheat gr ai n, and wheat hay from the metabolism studies. From 89

- 100 %

TRR were extracted from [ 14 

### C ] cyantraniliprole

- treated wheat gr ai n, wheat hay, beet foliage and

lettuce using the extraction procedures of the enforcement method

. The extraction procedur es of

the plant and livestock commodity analytical methods are similar to those used in the metabolism

studies except with the use of a Geno Grinder in place of Tekmar Tissue

- mizer probe. Also

submitted were validation data using samples of liver, muscle, milk, egg white

, and egg yolk.

The levels of extracted radioactivity obt ai ned using the residue method were equivalent of those

found in the metabolism extraction: 75.5% of liver, 92.7% for muscle, 104% for milk, 99.5% for

egg white, and 125% for egg yolk . These data are adequate to satisfy extraction efficiency

requirement for the plant and livestock methods.

The methods used for data collection in the field trials and processing studies (Method 15736)

and livestock feeding studies (Method 18844) are the same as the enforcement methods and

were shown to be adequate via concurrent recoveries for fortified samples. See field trial

sections for specific crops for recovery summaries.

Conclusions

. The submitted residue analytical method data are adequat e. Adequate validation

data have been provided for the data collection and the proposed enforcement method s

. Adequate ILV data have been submitted for the proposed enforcement method s

.

860.1360 Multiresidue Methods

Monograph for Cyantraniliprole, Section B.5.2.1 (MRIDs 48119928, 48119929, 48119933, 48119999, 48122526 )



### Cyantraniliprole has been evaluated using the multi

- residue methodologies (MRM)

. The analysis

of cyantraniliprole residues in crops was evaluated using US FDA protocol A through F. T he

results from this study indicate that cyantraniliprole residues cannot be analyzed using the FDA

multi

- residue method ology

.

Protocol A

Some of the test substances, cyantraniliprole and metabolites IN

- J9Z38, IN

- JCZ38, IN

- K7H19,

IN

- MLA84, IN

- MYX98, and I N

- N7B69, fluoresced by the methods outlined in Protocol A, but

none were capable of being evaluated further without modifying the methods; therefore,

recovery through the complete method was not performed.

Protocol B

The test substance was not evaluated t hrough Protocol B because it does not possess a carboxylic

acid or phenolic moiety.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 42 of 193 Protocol C

IN

- JCZ38

- 004 and IN

- K7H19

- 001 can be chromatographed and detected by module DG 18,

Level II (ECD with DB

- 225 type column). Module DG 18 was chosen as the most a ppropriate

determinative step for use in Protocols D, E, and F.

Protocols D, E, and F

The test substances proved insoluble in the solvents required by the Florisil cleanup methods;

therefore, testing was suspended.

EU Multiresidue Method

The DFG multiresidue method is adequate for determination of cyantranailiprole in plant

matrices.

Cyantraniliprole was extracted from the plant tissue according to extraction module E 1 (tomato),

E 2 (wheat grain), E 3 (orange), and E 7 for (almond) of th e DFG Method S 19 method. Extracts

of tomato, orange, wheat grain, and almond were analyzed by liquid chromatography with

electro spray ionization (MRM positive mode) and tandem mass spectrometry detection. The

DFG Method S 19, using gas chromatography for detection could not be applied to

cyantraniliprole as degradation of cyantraniliprole was observed under GC/MS conditions.

Recovery data from various matrix fortifications are summarized in Table 13.

Table 13. Fortification Recoveries for method DFG S19 for the determination of cyantraniliprole in

plant commodities

Matrix mass

transition

(m/z) fortification

level (mg/kg) number of

values Min (%) Max

(%) Mean

(%) RSD

(%)

Tomato 475

- > 286 unfortified 1* nd

0.01 5 93 101 98.2 3.2

0.1 5 111 119 114.6 2.7

475

- > 112 unfortified 1* nd

0.01 5 90 104 99.8 5.6

0.1 5 112 119 115.2 2.2

Wheat grain 475

- > 286 unfortified 2 nd**

0.01 5 92 100 96.8 3.5

0.1 5 89 108 101.0 7.2

475

- > 112 unfortified 2 nd*

0.01 5 91 103 96.0 5.9

0.1 5 91 110 102.6 7.2

O range 475

- > 286 unfortified 2 nd**

0.01 5 87 104 92.4 7.4

0.1 5 89 98 92.8 4.0

475

- > 112 unfortified 2 nd**

0.01 5 80 99 89.2 8.7

0.1 5 87 96 91.0 4.3Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 43 of 193 A lmond 475

- > 286 unfortified 2 nd**

0.01 5 101 107 104.2 2.9

0.1 5 96 107 102.4 4.3

475

- > 112 unfortified 2 nd**

0.01 5 100 114 106.6 5.3

0.1 5 95 110 103.2 5.5

* a blank control has been analyz ed with a content of 0.0037 or 0.0040 mg/kg, this sample was contaminated and not

representative of blank tomato.

** value found in the samples were below the LOD of 0.002 mg/kg



### Conclusions

. The above data are adequate to satisfy the FDA MRM data requirements. At the

same time, DFG S19 is adequate as a multi

- residue method in quantifying residues of

cyantraniliprole in plant commodities.

860.1380 Storage Stability

Monograph for Cyantraniliprole

, Sect ion B.7.6.2 , B.7.8.2, B.7.8.1 (MRIDs 48119993, 48120014, 48120015 )



### The stability of cyantraniliprole and metabolites IN

- F6L99, IN

- J9Z38, cyantraniliprole , IN

F6L99, INJ9Z38, IN

- JCZ38, IN

- K7H, IN

- MLA84, IN

- JCZ38, IN

- K7H, IN

- MLA, IN

- MYZ98,

IN

- N7B, IN

- N5M, IN

- MYX, IN

- N7B, and IN

- N5M under conditions of frozen storage were

studied for the following commodities: apple, peanut, dried beans, potato, and grape. The results

are summarized in Table 14

.

Table 14 : Summary of stability of residue components in representative plant matrices under frozen storage (≤

- 18°C).

Residue

component High

- water

content matrix High

- starch

content matrix High

- acid

content matrix High

- protein

content matrix High

- oil

content matrix

Crop Months Crop Months Crop Months Crop Months Crop Months

Cyantraniliprole Apple 24 Potato 24 Grapes 24 Dried

beans 18 Peanuts 18

IN

- F6L99 Apple 24 Potato 24 Grapes 24 Dried

beans 18 Peanuts 18

IN

- J9Z38 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts 24

IN

- JCZ38 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts Not

stable

IN

- K7H19 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts Not

stable

IN

- MLA84 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts 24

IN

- MYX98 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts 18

IN

- N7B69 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts 12

IN

- N5M09 Apple 24 Potato 24 Grapes 24 Dried

beans 24 Peanuts 24Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 44 of 193 Table 15 : Summary of Storage Conditions and Durations for Crop Field Trials, Rotational Crop

Field Trials, and Processing Studies

Matrix Storage

Temperature

(oC) Longest Actual Storage

Interval from Sampling

to Extraction Longest Actual

Storage

Interval from

Extraction to

Analysis Interval of

Demonstrated

Storage

Stability

Days Months Days Months Months

Crop Field Trials

Potato tubers

- 20±10 327 10.8 84 3.0 Potatoes (high

starch/root

vegetable: 24

months for all

analytes

Dry bulb onion

- 20±10 370 12.2

55 1.8 High water content

commodities: 24

months for all

analytes Green onion 220 7.2

Head/Leaf Lettuce

- 20±10 167 5.5

70 2.3 High water content

commodities: 24

months for all

analytes Celery 271 8.9

Spinach 184 6.0

Broccoli/Cauliflower

- 20±10 190 6.2

148 4.9 High water content

commodities: 24

months for all

analytes. Cabbage (RAC) 123 4.0

Cabbage (prepared for

consumption) 94 3.1

Mustard Greens 238 7.8

Broccoli/Cauliflower 190 6.2

Fruiting Vegetables

(peppers, tomatoes) - 20±10 236 7.8 86 2.8 High water content

commodities: 24

months for all

analytes

Fruiting Vegetables

(peppers, tomatoes)

Europe - 20 240 8.0

- - High water content

commodities: 24

months for all

analytes

Cucurbit Vegetables

(melon, cucumber,

summer squash) - 20±10 282 9.3 70 2.3 High water content

commodities: 24

months for all

analytes

Citrus fruits

(orange, grapefruit,

lemon) - 20±10 Peel/pulp:157

(for 98% of the

samples, with

less than 2%

stored for 370

days). 5.1

(for 98% of

samples, and

12.1 for the

rem ai nder) 46 1.5 High acid content

commodities: 24

months for all

analytes.

Apple

- 20±10 210 6.9

76 2.5 High water content

commodity: 24

months for all

analytes Pear 172 5.7

Stone fruits

(peach, plum, cherry) - 20±10 206 6.8 101 3.3 High water content

commodity: 24

months for all

analytes

Blueberry

- 20±10 114 3.7 4 0.1 High acid content

commodity: 24

months for all

analytesCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 45 of 193 Table 15 : Summary of Storage Conditions and Durations for Crop Field Trials, Rotational Crop

Field Trials, and Processing Studies

Matrix Storage

Temperature

(oC) Longest Actual Storage

Interval from Sampling

to Extraction Longest Actual

Storage

Interval from

Extraction to

Analysis Interval of

Demonstrated

Storage

Stability

Days Months Days Months Months

Tree nuts

(almond, pecan) - 20±10 188 6.2 37 1.2 High oil content

commodity: 24

months for IN

J9Z38, IN

- MLA84,

and IN

- N5M09; 18

months for parent,

IN

- FL6L99, and

IN

- MYX98; 12

months for IN

N7B69; not stable

for IN

- JCZ38 and

IN

- K7H19.

Cotton

- 20±10 237 7.8 88 2.9 High oil content

commodity: 24

months for IN

J9Z38, IN

- MLA84,

and IN

- N5M09; 18

months for parent,

IN

- FL6L99, and

IN

- MYX98; 12

month s for IN

N7B69; not stable

for IN

- JCZ38 and

IN

- K7H19.

Canola and Sunflower

- 20±10 194 6.4 112 3.7 High oil content

commodity: 24

months for IN

J9Z38, IN

- MLA84,

and IN

- N5M09; 18

months for parent,

IN

- FL6L99, and

IN

- MYX98; 12

months for IN

N7B69; not stable

for IN

- JCZ38 and

IN

- K7H19.

Rotational Crop Field Trials

Lettuce, radish, oats,

soybean - 20 655 22 76 2.5 High water, starch,

acid content: 24

months for

cyantraniliprole.

High protein, high

oil content:

18 months for

cyantraniliprole

Turnip, sugar beet,

garden beet, carrot,

pea, soybean, sweet

corn, sorghum, rice,

wheat, Bermuda grass,

alfalfa, bromegrass, - 20 364 12 148 5 High water, starch,

acid content: 24

months for

cyantraniliprole.

Hi gh protein, high

oil content:Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 46 of 193 Table 15 : Summary of Storage Conditions and Durations for Crop Field Trials, Rotational Crop

Field Trials, and Processing Studies

Matrix Storage

Temperature

(oC) Longest Actual Storage

Interval from Sampling

to Extraction Longest Actual

Storage

Interval from

Extraction to

Analysis Interval of

Demonstrated

Storage

Stability

Days Months Days Months Months

clover, bluegrass,

radish, peanut,

strawberry 18 months for

cyantraniliprole

Processing

Potato tubers and

processed commodities - 20±10 189 6.2 84 3.0 Potatoes (high

starch/root

vegetable: 24

months for all

analytes

Cooked Spinach

Cooking water - 20±10 163

104 5.4

3.4 70 2.3 High water content

commodities: 24

months for all

analytes

Tomatoes Processed

Commodities - 20±10 183 6.0 16 0.5 High water content

commodities: 24

months for all

analytes

Orange Processed

Commodities - 20±10 200 6.6 46 1.5 High acid content

commodities: 24

months for all

analytes.

Apple processed

commodities - 20±10 155 5.1 76 2.5 High water content

commodity: 24

months for all

analytes

Dried plums

- 20±10 101 3.3 68 2.2 High water content

commodity: 24

months for all

analytes

Cotton Processed

Commodities

- 20±10 148 4.9 88 2.9 High oil content

commodity: 24

months for IN

J9Z38, IN

- MLA84,

and IN

- N5M09; 18

months for parent,

IN

- FL6L99, and

IN

- MYX98; 12

months for IN

N7B69; not stable

for IN

- JCZ38 and

IN

- K7H19.



### Poultry matrix samples were stored at ca.

- 80 C (

- 112 F) for a maximum of 97 days.

Cyantraniliprole and its 7 metabolites (IN

- HGW87, IN

- J9Z38, IN

- JCZ38, IN

- K7H19,

IN

- MLA84, IN

- MYX98, and IN

- N7B69) were shown to be stable over a period of 9 9 days in

eggs. All muscle and s kin with fat samples were analyz ed within 20 days. All liver samples were

extracted on the day of collection and analyz ed. Stability in tissues was not investigated, but asCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 47 of 193 all tissue samples were stored less than 30 days in feeding studies, no data are required

.

Table 16

. Recovery data following storage in eggs

Analyte Time zero 99 days of frozen storage in eggs

%

Recovery Mean

recovery

(%) Fresh %

recovery Mean

fresh

recovery

(%) Stored

recovery

(%) Mean

stored

recovery

(%) Corrected Mean

Recovery in

Stored Samples

(%)

Cyantraniliprole 100, 96 98 101, 99 100 99, 102 101 101

IN

- HGW87 102, 97 99 97, 95 96 103, 105 104 108

IN

- J9Z38 100, 96 98 84, 87 85 83, 85 84 99

IN

- JCZ38 94, 90 92 94, 93 93 90, 92 91 98

IN

- K7H19 98, 92 95 95, 95 95 94, 97 95 100

IN

- MLA84 105, 100 103 72, 73 73 83, 80 81 111

IN

- MYX98 107, 102 105 98, 97 97 96, 99 98 101

IN

- N7B69 113, 105 109 82, 81 81 77, 80 79 98



### Ruminant matrix s amples were stored at ca.

- 80 C (

- 112 F) for a maximum of 42 days.

Cyantraniliprole and its 7 metabolites (IN

- HGW87, IN

- J9Z38, IN

- JCZ38, IN

- K7H19,

IN

- MLA84, IN

- MYX98, and IN

- N7B69) were shown to be stable ov er a period of 99 days in

milk. All mu scle and fat samples were analyz ed within 24 days. All liver and kidney samples

were extracted on the da y of collection and analyzed . Stability in tissues was not investigated,

but as all samples were stored less than 30 days in feeding studies, no data are required.

Table 17 : Recovery data following storage in milk

Analyte Time zero 99 days of frozen storage in milk

%

Recovery Mean

recovery

(%) Fresh %

recovery Mean

fresh

recovery

(%) Stored

recovery

(%) Mean stored

recovery (%) Corrected Mean

Recoveries in

Stored Samples

(%)

Cyantraniliprole 93, 99 96 104, 95 100 94, 104 99 99

IN

- HGW87 99, 105 102 112, 103 108 106, 119 113 105

IN

- J9Z38 90, 98 94 85, 69 77 64, 73 69 90

IN

- JCZ38 89, 92 90 99, 92 96 85, 100 93 97

IN

- K7H19 96, 100 98 105, 95 100 92, 106 99 99

IN

- MLA84 90, 97 93 66, 50 58 47, 66 56 97

IN

- MYX98 101, 105 103 101, 92 96 91, 104 98 102

IN

- N7B69 113, 119 116 97, 88 93 80, 94 87 94



### Conclusions

.

Cyantraniliprole was stable (70

– 120% recovery) for 24 months in high water content plant

matrices, high starch content matrices, and high acid content matrices and was stable for 18

months in high protein content matrices and high oil content matrices. A similar stability pattern

was observed for the metabolites, except IN

- JCZ38 and IN

- K7H19 were not stable in a high oil

content matrix. These results cover the five major types of matrices a nd may be extrapolated to

all plant commodity primary and processed matrices. These intervals of demonstrated storage

stability cover the storage intervals of samples from the primary crop field trials, rotational crop

field trials, and processing studies . No corrections need be applied to the residue study results.

Cyantraniliprole and seven metabolites were stable (70

– 120% recovery) for 99 days in eggCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 48 of 193 samples and milk samples stored frozen at

- 80 C

. This interval is longer than the storage

intervals for egg and milk samples from the feeding studies, and storage stability has been

demonstrated. As tissue samples were analyzed immediately or within 30 days, storage stability

data are not required, and no correction of residue values from livestock com modities are

needed.

860.1400 Water, Fish, and Irrigated Crops

There are no proposed uses that are relevant to this guideline topic

.

860.1460 Food Handling

DuPont has proposed use of HG

- W86

- SC, an 18.66% or 1.67 lb ai /gallon suspension concentrate

formulation for use in food handling establishments. This includes use when food/feed is

present. No studies were submitted in support of the use.

Conclusion:

Cyantraniliprole may not be used in food and feed han d ling establishments, pending submissi on

and evaluation of the appropriate studies per guideline 860.1460.

860.1480 Meat, Milk, Poultry, and Eggs

Monograph for Cyantraniliprole

, Section s B.7.8.1 and B.7.8.2 (MRIDs 48120014 and 48120015 )



### There are livestock feedstuffs associated with the proposed uses of cyantraniliprole

, and the

following one s are considered important and represent maximum dietary burdens : almond hulls,

cotton gin byproducts, cotton meal, field corn gr ai n (rotation), grass hay (r otation), potato culls,

soybean hay (rotation), and sunflower meal

. As a result, t he estimated dietary burdens of

cyantraniliprole to livestock, as presented in Table 9 , are conservative . The estimated dietary

burdens are 0.37 ppm for beef cattle, 0.41 pp m for d ai ry cattle, 0.01 ppm for poultry, and 0.011

ppm for swine. The submitted feeding s tudies with cattle and poultry are summarized below.

Table 18

. Ca lculation of Dietary Burdens of Cyantraniliprole Residues to Livestock.

Feedstuff Type 1 % Dry

Matter 2 % Diet 2 Expected Residue Dietary Contribution

(ppm) 3 Value used ppm

Beef Cattle : 15% R, 80% CC, 5% PC

Cotton, gin byproducts R 90 5 Median 3.15 0.175

Grass, hay (rotation) R 90 10 HAFT 0.23 0.026

Potato, culls CC 20 30 HAFT 0. 11 0.165

Corn, field, gr ai n

(rotation) CC 88 50 Median <0.01 0.005

Cotton, meal PC 89 5 PF x Median 0.016 0.000

TOTAL BURDEN

-- -- 100

-- -- 0.37

D ai ry Cattle : 45% R, 45% CC, 10% PC

Almond, hulls R 90 10 Median 1. 60 0.17 8

Soybean, hay (rotation) R 89 20 HAFT 0.63 0.142

Grass, hay (rotation) R 90 15 HAFT 0.23 0.038Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 49 of 193 Table 18

. Ca lculation of Dietary Burdens of Cyantraniliprole Residues to Livestock.

Feedstuff Type 1 % Dry

Matter 2 % Diet 2 Expected Residue Dietary Contribution

(ppm) 3 Value used ppm

Potato, culls CC 20 10 HAFT 0.11 0.055

Corn, field, gr ai n

(rotation) CC 88 35 Median <0.01 0.004

Cotton, meal PC 89 10 PF x Median 0.016 0.0018

TOTAL BURDEN

-- -- 100

-- -- 0.4 2

Poultry : 75% CC, 25% PC

Corn, field, gr ai n

(rotation) CC 88 75 Median <0.01 0.007

Cotton , meal PC 89 20 PF x Median 0.016 0.003

Sunflower , meal PC 92 5 PF x Median 0.007 0.000

TOTAL BURDEN

-- -- 100

-- 0.01

Swine : 85% CC, 15% PC

Corn, field, gr ai n

(rotation) CC 88 85 Median <0.01 0.0085

Cotton, meal PC 89 15 PF x Median 0.016 0.0024

TOTAL BURDEN

-- -- 100

-- 0.011

1 R: Roughage; CC: Carbohydrate concentrate; PC: Protein concentrate.

2 OCSPP 860.1000 Table 1 Feedstuffs (June 2008).

3 Contribution = ([expected residue /% DM] X % diet) for beef and d ai ry cattle; contribution = ([expected residue]

X % diet) for swine and poultry.



### Cattle

MRID 48120014 : Four treatment groups of three d ai ry cows each were dosed orally in gelatin

capsules (twice d ai ly) for 2 8 consecutive days with cyantraniliprole at 3, 10, 30, and 100 ppm in

the animal diet on a dry weight basis

. Two cow s w ere not dosed to serve as control s

. An

additional 3 cows were dosed at 100 ppm for 28 days to obt ai n depuration data. The dosing

levels of the groups represent 8.1x, 27x, 81x, and 270x , respectively, the dietary burden to beef

cattle and 7.3x, 24x, 73x, and 244x , respectively, the d ietary burden to d ai ry cattle.

Cows were milked twice d ai ly and samples from the afternoo n samplings were combined with

samples from the next morning

. Milk samples were collected from individual cows d ai ly and

milk collected on Days

- 1, 1,3, 5, 10, 14, 21, 28 were analyzed. Residues in milk reached a

plateau after approximately of 5 days and did not appear to accumulate in milk. Cows (except for

the depuration group) were sacrificed less than 24 hours after the final dose. Samples of liver,

muscle (loin and flank or round leg)

, kidney, and fat ( omental, perirenal, and subcutaneous) were

taken for analysis. Samples of cream (milk fat) and skim milk were generated from milk

samples collected on study D ay 14 and 21

.

Samples of cattle ( and poultry ) matrices were analyzed for residues of cyantraniliprole and seven

of its metabolites using HPLC/MS/MS (DuPont

- 18844)

. Residues were expressed in terms of

each analyte. This method wa s adequate for data collection based on acceptable method

recoveries : 80

- 102% for parent, 79

- 100% for IN

- HGW87, 84

- 94% for IN

- J9Z38, 80

- 94% for

IN

- JCZ38, 87

- 101% fo r IN

- K7H19, 80

- 97% for IN

- MLA84, 84

- 105% for IN

- MYX98, and 87

-Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 50 of 193 98% for IN

- N7B69. The validated LOQs in each matrix were 0.01 ppm for cyantraniliprole and

metabolites

.

Milk and tissue samples were stored frozen ( ≤

- 8 0 oC ) for a maximum of 42 days. Freezer storage

stability data demonstrated that residues of cyantraniliprole and metabolites are stable in fortified

samples of milk at

- 80 oC for up to 99 days. All muscle and fat samples were analyzed within 24

days, and all liver and kidney samples were extra cted on the day of collection and analyzed.

The results of the cattle feeding study are summarized in Table 19

. Residues were highest in

liver, followed by kidney, fat and muscle. Residue levels in tissues were dose dependent.

In whole milk, the maximu m average cyantraniliprole residues were 0.030, 0.11, 0.25, and 0.71

ppm, respectively, in the 3, 10, 30 an d 10 0 ppm dose groups. The metabolite that had the highest

residue s in milk was IN

- N7B69 , present at 0.028, 0.074, 0.17 and 0.28 ppm, respectively.

R esidue levels in cream were higher than in skim milk.

Table 19. Residues of cyantraniliprole and metabolites in milk products from lactating cows

dosed with cyantraniliprole for 28 days

Matrix Analyte Values in milk represent the highest pm + am pooled sample value

3 mg/kg 10 mg/kg 30 mg/kg 100 mg/kg

Maximum Residue (Day 5

– 28) ppm

Milk Cyantraniliprol

e 0.030 0.11 0.25 0.71

IN

- HGW87 ND ND <0.01 <0.01

IN

- J9Z38 <0.01 <0.01 0.010 0.034

IN

- JCZ38 ND ND ND ND

IN

- K7H19 ND ND ND ND

IN

- MLA84 <0.01 <0.01 <0.01 <0.01

IN

- MYX98 <0.01 <0.01 0.025 0.085

IN

- N7B69 0.028 0.074 0.17 0.28

Study day (100 mg/kg) 14 21 14 21 14 21 14 21

Cream Cyantraniliprol

e 0.072 0.059 0.2 0.15 0.63 0.46 1.9 1.7

IN

- HGW87 ND ND ND ND <0.01 <0.01 <0.01 <0.01

IN

- J9Z38 0.014 0.011 0.032 0.027 0.085 0.066 0.37 0.31

IN

- JCZ38 ND ND ND ND ND ND ND ND

IN

- K7H19 ND ND ND ND ND ND ND ND

IN

- MLA84 <0.01 <0.01 <0.01 <0.01 0.026 0.021 0.039 0.041

IN

- MYX98 <0.01 <0.01 <0.01 <0.01 0.020 0.023 0.066 0.079

IN

- N7B69 0.019 0.022 0.048 0.053 0.12 0.14 0.19 0.25

Skim milk Cyantraniliprol

e 0.019 0.014 0.049 0.039 0.15 0.13 0.47 0.47

IN

- HGW87 ND ND ND ND ND ND <0.01 <0.01

IN

- J9Z38 ND ND ND ND ND <0.01 <0.01 <0.01

IN

- JCZ38 ND ND ND ND ND ND 0.030 ND

IN

- K7H19 ND ND ND ND ND ND ND ND

IN

- MLA84 ND ND ND ND <0.01 ND <0.01 <0.01

IN

- MYX98 <0.01 <0.01 <0.01 <0.01 0.022 0.020 0.066 0.051

IN

- N7B69 0.019 0.020 0.047 0.057 0.12 0.13 0.22 0.18

ND = Not detected (<0.002 mg/kg)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 51 of 193 Table 20. Residues of cyantraniliprole and metabolites in tissues from lactating cows dosed with

cyantraniliprole for 28 days

Matrix Analyte Mean (Max) residues of cyantraniliprole and metabolites in tissues

3 mg/kg 10 mg/kg 30 mg/kg 100 mg/kg

Muscle Cyantraniliprole <0.01

(0.011) 0.026

(0.037) 0.071

(0.092) 0.28

(0.33)

IN

- HGW87 ND ND ND <0.01

IN

- J9Z38 ND <0.01 0.010

(0.018) 0.027

(0.043)

IN

- JCZ38 ND ND ND ND

IN

- K7H19 ND ND ND ND

IN

- MLA84 ND ND <0.01 <0.01

IN

- MYX98 ND <0.01 <0.01 0.01

(0.011)

IN

- N7B69 ND <0.01 <0.01 <0.01

Liver Cyantraniliprole 0.054

(0.066) 0.15

(0.16) 0.46

(0.60) 1.7

(2.1)

IN

- HGW87 ND ND <0.01 0.010

(0.010)

IN

- J9Z38 ND <0.01 <0.01 0.015

(0.026)

IN

- JCZ38 ND ND ND <0.01

(0.011)

IN

- K7H19 ND ND ND ND

IN

- MLA84 0.032

(0.043) 0.075

(0.099) 0.22

(0.29) 0.41

(0.57)

IN

- MYX98 ND <0.01 <0.01

(0.026) 0.025

(0.025)

IN

- N7B69 <0.01

(0.01) 0.021

(0.024) 0.042

(0.046) 0.076

(0.079)

Kidney Cyantraniliprole 0.023

(0.031) 0.084

(0.14) 0.20

(0.25) 0.73

(0.89)

IN

- HGW87 ND ND <0.01 0.011

(0.012)

IN

- J9Z38 ND <0.01 <0.01

(0.013) 0.024

(0.031)

IN

- JCZ38 ND ND ND ND

IN

- K7H19 ND ND ND ND

IN

- MLA84 <0.01

(0.011) 0.013

(0.017) 0.041

(0.044) 0.099

(0.13)

IN

- MYX98 <0.01 <0.01 0.034

(0.039) 0.14

(0.15)

IN

- N7B69 0.012

(0.015) 0.031

(0.031) 0.071

(0.081) 0.12

(0.15)

Fat Cyantraniliprole 0.014

(0.015) 0.042

(0.066) 0.12

(0.15) 0.51

(0.58)

IN

- HGW87 ND ND ND ND

IN

- J9Z38 0.01

(0.012) 0.023

(0.031) 0.082

(0.12) 0.38

(0.45)

IN

- JCZ38 ND ND ND ND

IN

- K7H19 ND ND ND ND

IN

- MLA84 ND <0.01 <0.01 <0.01

IN

- MYX98 ND <0.01 <0.01 <0.01Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 52 of 193 Table 20. Residues of cyantraniliprole and metabolites in tissues from lactating cows dosed with

cyantraniliprole for 28 days

Matrix Analyte Mean (Max) residues of cyantraniliprole and metabolites in tissues

3 mg/kg 10 mg/kg 30 mg/kg 100 mg/kg

IN

- N7B69 <0.01 <0.01 0.010

(0.012) 0.020

(0.024)

ND = Not detected (<0.002 mg/kg)



### The data indicate that quantifiable residues of cyantraniliprole occur in all cattle matrices (except

in muscle at the lowest 3 ppm dose level) at all dosing level s. Transfer factors for

cyantraniliprole and metabolites were calculated for each matrix at each feeding level, using the

maximum observ ed residue level for tissues . The calculated tran sfer factors are presented in

Table 21

.

Table 21. Maximum (Mean) Residues of Cyantraniliprole in Cattle Commodities by Feeding Level.

Cattle Matrix 3.0 ppm 1 0 ppm 30 ppm 1 00 ppm

Cyantraniliprole (MW=473.7)

Milk 0.0 3 0. 11 0. 25 0. 7 1

Muscle 0.011

(< 0.01) 0.0 37

(0.026) 0.0 92

(0.071 ) 0. 3 3

(0.28)

Liver 0.066

(0.054) 0.16

(0.15) 0.60

(0.46) 2.1

(1.7)

Kidney 0.031

(0.023) 0.14

(0.084) 0.25

(0.20) 0.89

(0.73)

Fat 1 0.015

(0.014) 0.066

(0.042) 0.15

(0.12) 0.58

(0.51)

IN

- J9Z38 (MW=491.7)

Milk <0.01 < 0.01 0.010 0.034

Muscle <0.002 <0.01 0.018

(0.010) 0.043

(0.027)

Liver <0.002 <0.01 <0.01 0.026

(0.015 )

Kidney <0.002 <0.01 0.013

(<0.01 ) 0. 031

(0.024 )

Fat 1 0.012

(0.01 ) 0.0 31

(0.023 ) 0.1 2

(0.08 2) 0. 4 5

(0.38 )

IN

- MLA84 (MW=441.7)

Milk <0.01 <0.01 < 0.01 <0.01

Muscle <0.002 <0.002 <0.01 <0.01

Liver 0.043

(0.032) 0.099

(0.075) 0.29

(0.22) 0.57

(0.41)

Kidney 0.011

(<0.01) 0.017

(0.013) 0.044

(0.041) 0.13

(0.099)

Fat 1 <0.002 <0.01 <0.01 <0.01

IN

- MYX98 (MW=489.7)

Milk <0.01 <0.01 0.025 0.085

Muscle <0.002 <0.01 <0.01 0.011

(0.01)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 53 of 193 Table 21. Maximum (Mean) Residues of Cyantraniliprole in Cattle Commodities by Feeding Level.

Cattle Matrix 3.0 ppm 1 0 ppm 30 ppm 1 00 ppm

Liver <0.002 <0.01 0.026

(<0.01) 0.025

(0.025)

Kidney <0.01 <0.01 0.039

(0.034) 0.15

(0.14)

Fat 1 <0.002 <0.01 <0.01 <0.01

IN

- N7B69 (MW=489.7)

Milk 0.028 0.074 0.17 0.28

Muscle <0.002 <0.01 <0.01 <0.01

Liver 0.01

(<0.01) 0.024

(0.021) 0.046

(0.042) 0.079

(0.076)

Kidney 0.015

(0.012) 0.031

(0.031) 0.081

(0.071) 0.15

(0.15)

Fat 1 <0.01 <0.01 0.01 0.02

1 Includes omental, perirenal, and subcutaneous fat.

Table 22

. Calculated transfer factors in cattle. 1 ,2

Commodity 3.0 ppm 10 ppm 30 ppm 100 ppm

Cyantraniliprole

Milk 0.01 0.011 0.0083 0.0071

Muscle 0.0037 0.0037 0.0031 0.0033

Liver 0.022 0.016 0.02 0.021

Kidney 0.01 0.014 0.0083 0.0089

Fat 0.005 0.0066 0.005 0.0058

IN

- J9Z38

Milk nd 1 nd 0.00033 0.00034

Muscle nd nd 0.0006 0.00043

Liver nd nd nd 0.00026

Kidney nd nd 0.00043 0.00031

Fat 0.004 0.0031 0.004 0.0045

IN

- MLA84

Milk nd nd nd nd

Muscle nd nd nd nd

Liver 0.014 0.0099 0.01 0.0057

Kidney 0.0037 0.0017 0.0015 0.0013

Fat nd nd nd nd

IN

- MYX98

Milk nd nd 0.00083 0.00085

Muscle nd nd nd 0.00011

Liver nd nd 0.00087 0.00025

Kidney nd nd 0.0013 0.0015

Fat nd nd nd nd

IN

- N7B69

Milk 0.0093 0.0074 0.0057 0.0028

Muscle nd nd nd ndCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 54 of 193 Table 22

. Calculated transfer factors in cattle. 1 ,2

Commodity 3.0 ppm 10 ppm 30 ppm 100 ppm

Liver 0.0033 0.0024 0.0015 0.00079

Kidney 0.005 0.0031 0.0027 0.0015

Fat nd nd 0.00033 0.0002

1 Transfer factor calculated by dividing residue value by feeding level. nd = not determined; residues were below

the LOQ.

2 For all tissues, the transfer factor was calculated using the maximum residue value obse rved at the specified

feeding level.



### Poultry

MRID 48120015 : F our treatment groups each consisting of 1 0 laying hens were dosed orally,

via feed, for 28 consecutive days with cyantraniliprole at target dose rates of 3, 10 and 3 0 ppm

feed. Ten birds received capsules cont ai ning no cyantraniliprole as control. Additionally, 10

birds were dosed at 30 ppm for 28 days to obt ai n depuration data. The dosing levels of the

groups represent 300 x, 100 0 x, and 300 0 x, respectively, the dietary bu rden to poultry.

Eggs were collected from all group s d ai ly during the dosing period. E ggs were collected twice

d ai ly. Samples from the afternoon collection were combined with samples from the next

morning. Egg samples on s tudy d ays

- 3 , 1

- 7 , 1 0 , and 2 7 from the 4 treatment groups were

analyzed. Egg yolk and egg white samples were prepared from eggs collected on days 27 and 28.

Bird s were sacrificed 4

- 6 hours after the final dose (except f or the depuration group ).

Samples of liver (entire organ), muscle (leg and breast) , and abdominal fat with skin were

collected for analysis. All samples were stored frozen at ca

- 80 oC until analysis. Liver was

extracted on the day of collection. Muscle and skin with fat were stored frozen for a maximum of

20 days. Most samples were analyzed within 30 days and all samples were analyzed within 3

months of collection . Storage stability data for residues of parent plus metabolites in eggs

indicate that these compounds are stable under frozen for 97 days.

Samples of poultry (and cattle) matrices were analyzed for residues of cyantraniliprole and seven

of its metabolites using HPLC/MS/MS (DuPont

- 18844). Residues were expressed in terms of

each analyte. This method was adequate for data collection based on acceptable method

r ecoveries: 80

- 102% for parent, 79

- 100% for IN

- HGW87, 84

- 94% for IN

- J9Z38, 80

- 94% for

IN

- JCZ38, 87

- 101% for IN

- K7H19, 80

- 97% for IN

- MLA84, 84

- 105% for IN

- MYX98, and 87

-

98% for IN

- N7B69. The validated LOQs in each matrix were 0.01 ppm for cyantraniliprole a nd

metabolites.

Residues in whole eggs reached a plateau after ca. 3 days of dosing. Eggs seemed to cont ai n the

highest amount of residues, followed by liver, fat with skin and muscle. Parent residues were the

highes t in eggs, followed by IN

- J9Z38, IN

- M LA84, and IN

- HGW87 (decreasing order). The

results of the poultry feeding study are summarized in Table 23

.

Although cyantraniliprole was not found in liver in the poultry metabolism study, it was found as

a major component of the residue in the feeding study, along with metabolites IN

- MLA84 andCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 55 of 193 IN

- MYX98. This may be a reflection of the longer duration of the f eeding study as compared to

the metabolism study, 28 days versus 1 4 day s

.

Table 23. Residues of cyantraniliprole and metabolites in eggs

Matrix Analyte 3 mg/kg 10 mg/kg 30 mg/kg

Mean residue (mg/kg)

Eggs Cyantraniliprole 0.082 0.17 0.80

IN

- HGW87 0.0053 0.0069 0.016

IN

- J9Z38 0.039 0.077 0.41

IN

- JCZ38 <0.002 <0.002 <0.002

IN

- K7H19 <0.002 ND ND

IN

- MLA84 0.016 0.038 0.12

IN

- MYX98 0.014 0.035 0.10

IN

- N7B69 <0.002 <0.002 0.0029

Table 24 : Average residues of cyantraniliprole and metabolites in tissues

Matrix Analyte 3 mg/kg 10 mg/kg 30 mg/kg

Mean residue (mg/kg)

Muscle Cyantraniliprole 0.0034 0.0093 0.025

IN

- HGW87 <0.002 0.0052 0.011

IN

- J9Z38 ND ND ND

IN

- JCZ38 ND ND ND

IN

- K7H19 ND ND ND

IN

- MLA84 <0.002 <0.002 <0.002

IN

- MYX98 0.0041 0.012 0.025

IN

- N7B69 <0.002 <0.002 0.0026

Liver Cyantraniliprole 0.017 0.041 0.13

IN

- HGW87 0.0076 0.028 0.059

IN

- J9Z38 <0.002 <0.002 <0.002

IN

- JCZ38 0.0030 0.0094 0.0060

IN

- K7H19 ND ND ND

IN

- MLA84 0.015 0.043 0.096

IN

- MYX98 0.023 0.068 0.19

IN

- N7B69 0.0067 0.013 0.045

Skin with fat Cyantraniliprole 0.0093 0.033 0.080

IN

- HGW87 <0.002 0.0078 0.015

IN

- J9Z38 0.0029 0.0064 0.018

IN

- JCZ38 ND ND ND

IN

- K7H19 ND ND ND

IN

- MLA84 <0.002 0.0034 0.0080

IN

- MYX98 0.0037 0.015 0.027

IN

- N7B69 <0.002 <0.002 0.0030

ND

– Not DetectedCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 56 of 193 The results of the poultry feeding study are summarized in Table 25

. Residues of

cyantraniliprole in eggs and tissues were below 0.1 ppm in all samples at the lowest dose group

of 3 ppm (300X dietary burden)

. The parent compound was a major or the major residue.

Residue levels were dose dependent.

Table 25

. Maximum Residues of Cyantraniliprole in Hen Commodities by Feeding Level.

Hen Matrix 3.0 ppm 10 ppm 30 ppm

Cyantraniliprole (MW=473.7)

Eggs 0.082 0.17 0.80

Muscle 0.0034 0.0093 0.025

Liver 0.017 0.041 0.013

Skin with fat 0.0093 0.033 0.080

IN

- J9Z38 (MW=491.7)

Eggs 0.039 0.077 0.41

Muscle ND ND ND

Liver <0.002 <0.002 <0.002

Skin with fat 0.0029 0.0064 0.018

IN

- MLA84 (MW=441.7)

Eggs 0.016 0.038 0.12

Muscle <0.002 <0.002 <0.002

Liver 0.0 15 0.043 0.096

Skin with fat <0.002 0.0034 0.0080

IN

- MYX98 (MW=489.7)

Eggs 0.014 0.035 0.10

Muscle 0.0041 0.012 0.025

Liver 0.023 0.068 0.19

Skin with fat 0.0037 0.015 0.027

IN

- N7B69 (MW=489.7)

Eggs <0.002 <0.002 0.0029

Muscle <0.002 <0.002 0.0026

Liver 0.0067 0.013 0.045

Skin with fat <0.002 <0.002 0.0030



### Transfer factors for cyantraniliprole and its major metabolites were calculated for each matrix at

each feeding level, using the maximum observed residue level for eggs and tissue. The

calculated transfer factors are presented in Table 26

.

Table 26

. Calculated transfer factors in poultry. 1 ,2

Commodity 3.0 ppm 10 ppm 30 ppm

Cyantraniliprole

Eggs 0.027 0.017 0.027

Muscle 0.0011 0.00093 0.00083

Liver 0.0057 0.0041 0.00043

Skin with fat 0.0031 0.0033 0.0027

IN

- J9Z38Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 57 of 193 Table 26

. Calculated transfer factors in poultry. 1 ,2

Commodity 3.0 ppm 10 ppm 30 ppm

Eggs 0.013 0.0077 0.014

Muscle nd 1 nd nd

Liver nd nd n d

Skin with fat 0.00097 0.00064 0.0006

IN

- MLA84

Eggs 0.0053 0.0038 0.004

Muscle nd nd n d

Liver 0.005 0.0043 0.0032

Skin with fat n d 0.00034 0.00027

IN

- MYX98

Eggs 0.0047 0.0035 0.0033

Muscle 0.0014 0.0012 0.00083

Liver 0.0077 0.0068 0.0063

Skin with fat 0.0012 0.0015 0.0009

IN

- N7B69

Eggs nd nd 0.0001

Muscle nd n d 0.000087

Liver 0.0022 0.0013 0.0015

Skin with fat nd n d 0.0001

1 Transfer factor calculated by dividing residue value by feeding level. nd = not detected; residues wer e below the

LOQ.

2 For all tissues, the transfer factor was calculated using the maximum residue value observed at the specified

feeding level.



### Conclusions

. The submitted studies are adequate to satisfy data requirements

. The data indicate

that tolerances for cyantraniliprole residues of conc ern are needed for milk, and fat, meat, and

meat byproducts of cattle, goat, horse, and sheep egg, but not for eggs, poultry, and hogs

.

The maximum anticipated residues of cyantraniliprole were calculated using the estimated

dietary burdens for cyantran iliprole (Table 18) and the maximum transfer factors for

cyantraniliprole (Table 22 and Table 26 ) and are reported in Table 27 (cattle , poultry, and swine

commodities)

.

Table 27 . Maximum Anticipated Levels of Cyantraniliprole in Livestock Commodities Following Dosing at 1x

the Dietary Burden.

Livestock Estimated Dietary

Burden

( ppm ) Matrix Cyantraniliprole (ppm) Recommended Tolerance

Level ( ppm )

Cattle 0.4 2 1 Milk 0.004 6 0.01

Muscle 0.001 6 0.01

Liver 0.009 2 0.01

Kidney 0.005 9 0.01

Fat 0.002 8 0.01

Poultry 0.01 1 Egg 0.00027 None

Muscle <0.00010 None

Liver <0.00010 None

Skin with f

at <0.00010 NoneCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 58 of 193 Swine 0.011 1 Muscle <0.00010 None

Liver 0.00024 None

Kidney 0.00015 None

Fat <0.00010 None

1 Maximum estimated dietary burden s for cattle, poultry, or swine used to ca lculate maximum anticipated residues

in ruminant s , poultry, or hog s

.



### Based on the maximum anticipated residues, HED concludes that a tolerance of 0.01 ppm i s

appropriate for the sole residues of cyantranilip role in milk, and fat, meat, and meat byproducts

of c attle, goat, horse, and sheep . However, the residue transfer in eggs, poultry and hogs is

negligible such that tolerances are not needed in these commodities (a §180. 6(a ) ( 3) situation).

The residue de finition for human dietary risk assessment includes cyantraniliprole and the four

metabolites listed in Tables 21 and 26 . The following levels are estimated in cattle commodities

based on the transfer factor at 3.0 ppm: IN

- J9Z38 in fat (0.0017 ppm ) ; IN

- M LA84 in liver

(0.0059 ppm) and in kidney (0.0016 ppm) ; and IN

- N7B69 in milk (0.0039 ppm), in liver (0.0014

ppm), and in kidney (0.0021 ppm). While these will significantly increase the total exposure

relative to cyantraniliprole only, the total exposure r em ai ns <0.01 ppm (LOQ) for each

commodity, except liver (≈0.017 ppm)

The following levels are estimated in poultry commodities based on the transfer coefficient at 3.0

ppm: IN

- J9Z38 in eggs (0.00013 ppm) and in skin with fat (0.0000097 ppm); IN

- MYX98 in

eggs (0.000047 ppm), in muscle (0.000014 ppm), in liver (0.000077 ppm) , and in skin with fat

(0.000012 ppm); IN

- MLA84 in eggs (0.000053 ppm) and in liver (0.00005 ppm), and IN

- N7B69

in liver (0.000022 ppm). Values for each metabolites in all poultry commodities are <0.00010

excep t IN

- J9Z38 in eggs at 0.00013 ppm , or about 5 0% of the cyantraniliprole concentration in

eggs.

8 60.1500 Crop Field Trials

Monograph for Cyantraniliprole , Sections B.7. 1.4

– B.7.1.12 NAFTA Field Trials; B.7.6.1.5 (MRIDs 4812007

- 48120011, 48120016

- 48120023, 48120034, 48120037

- 4120038 )



### The registrant submitted crop field trial data from field trials conducted in North America with a

variety of crops and greenhouse data from the EU for peppers. The relevant studies are

described below

, and the results are summarized in Table 28 through Table 126.

For the North American field trials, the petitioner collected r esidue data for cyantraniliprole and

several metabolites

.

The Organization for Economic Cooperation and Development (OECD) tolerance calculation

procedure was utilized for determining appropria te tolerance levels based on the submitted crop

field trial data for raw agricultural crop commodities. All tolerance calculat ions are presented in

Appendix I I

.

An adequate number of trials with appropriate geographical distribution were submitted for each

crop under consideration (Appendix III) . Storage stability data were adequate to cover the

intervals of all crop field trial samples. Acceptable analytical methods were used to analyze theCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 59 of 193 crop commodities, with acceptable concurrent recoveries demo nstrated at appropriate

fortification levels.

Various crops

Cyantraniliprole is the major component of the residues in raw agricultural crop commodities,

although multiple analytes were determined for the field trial commodities

. Generally

metabolites co ntributed very litte (0

– 3%) of the cyantraniliprole concentration in the

commodities analyzed. Formulation bridging trials on a representative group of crops have

shown comparable residues among formulation types and methods of application, with limited

exception. In comparative residue testing of varied application methods, generally, foliar

applications have shown higher residues than soil applications and residues for crops grown

under protection (greenhouse) have shown higher resi dues than the same crops grown i n the

open field. An exception is seed treatment of potato pieces, where seed treatment plus foliar

treatment (total application rate 0.4 lb ai/acre) yielded higher residues than foliar only treatment

(total application rate 0.4 lb ai/acre).

Per trial conditions and results for each crop are detailed in the Mongraph. The per trial results

may also be seen in the tables associated with the OECD statistical calculation of the tolerance

(Appendix II). Summary results are presented here. Tabl es showing the number and

geographic distribution of trials are given in Appendix III.

Tuberous and Corm Vegetables ( Crop Sub

- Group 1C)

Twenty

- one potato field trials were conducted in the US and Canada, 3 of which were for

processing studies and 1 of which was a decline trial. All trial sites included a treatment plot that

received a seed treatment of Cyantraniliprole 625 g/L FS and one late season foliar application of

Cyantraniliprole 100 g/L OD. Nineteen tests included a treatment plot t hat received three late

season foliar applications of Cyantraniliprole 100 g/L OD. Four tests included a treatment plot

that received three late season foliar applications of Cyantraniliprole 100 g/L SE. One trial

employed in

- furrow application of Cyantr aniliprole 200 g/L SC at planting, followed by one late

season foliar application of Cyantraniliprole 100 g/L OD. The crop growth stage at the time of

foliar applications at the trial sites ranged from BBCH 43

- 97. For foliar applications, retreatment

int ervals were 4

– 6 days.

Sites with seed treated at the rate of 13.5 g ai /100 kg of seed with 2200 kg seed/ ha planting

density (approximately 0.268 lb ai /A or 300 g ai /ha , or 1.5 x proposed label rate ) also received a

single application at approximately 0.134 lb ai /A (150 g ai /ha) for a seasonal maximum of

approximately 0.402 lb ai /A/season (450 g ai /ha/season). Each foliar or furrow/foliar treated plot

applied a seasonal maximum of approximately 0.402 lb ai /A/season (450 g ai /ha/season). For

each foliar application, adjuvant was added to the spray mixture. Commodity samples were

harvested at commercial maturity after the second or third applications.

Samples were analyzed by DuPont Method 15736, R evision No. 1. Samples were extracted with

acetonitrile/water (9/1, v/v) and analyzed by LC/MS

- MS. The LOQ was 0.010 ppm for each

analyte determined. Untreated control samples spiked with 0.010

– 0.10 mg/kg cyantraniliprole

and metabolites were analyzed co ncurrently with the treated samples to verify method

performance. Recoveries for all analyte/matrix combinations ranged from 68

– 132%, with mostCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 60 of 193 recoveries between 70 and 120%. Although some of the recoveries are outside of the criteria

range, there is s ufficient data to validate the performance of the analytical method.

Table 28

. Summary Statistics for Potatoes from Seed + FoliarTreatments. 1

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate: lb ai /A

(g ai /ha) PHI

(days)

DuPont

27583 Potato

Tubers 625 g/L FS

+ 100 g/L

OD Seed +

Foliar 1 seed at

planting + 1

Foliar 0.332

- 0.414

(372

- 465) 6

- 8

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 42 42 42 42 42 42 42

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.024 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.016 0.010 0.010 0.010 0.010 0.010

Median 0.011 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.024 0.011

-- -- -- -- --

SDEV 0.025 0.002

-- -- -- -- --

1 Includes one in

- furrow treatment ( 200 g/L SC) plus one foliar treatment (100 g/L OD, total rate 0.425 lb ai /ac.



### Table 2 9 . Summary Statistics for Potatoes from 3 Foliar Applications.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate: lb ai /A

(g ai /ha) PHI

(days)

DuPont

27583 Potato

Tubers 100 g/L OD Foliar 3 (428

- 462) 6

- 8

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 38 38 38 38 38 38 38

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.034 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.034 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.012

-- -- -- -- -- --

SDEV 0.006

-- -- -- -- -- --



### Table 30

. Summary Statistics for Potatoes from Bridging Trials with 100g/L SE.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate: lb ai /A

(g ai /ha) PHI

(days)

DuPont

27583 Potato

Tubers 100 g/L SE Foliar 3 0.399

- 0.406 (447

- 455) 6

- 7

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 10 10 10 10 10 10 10

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.035 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.016

-- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 61 of 193 SDEV 0.009

-- -- -- -- -- --

a EP = End

- use product

b n = Number of samples ; Mi n. = Minimum individual sample residue; Max. = Maximum individual s ample residue; HAFT =

Highest average residue from field trial s ; Median = Median of average sample residues; Mean = Mean of average sample

residues

SDEV. = Standard deviation of mean



### The decline studies showed residues at or near the LOQ at all intervals.

Conclusions:

The Canada/US field potato field trial results were generated using an adequate data collection

method

. Adequate storage stability data are av ai lable on diverse crop type. The number and

geographic distribution of trials are generally in accordance with OCSPP harmonized test

guideline 860.1500 (Appendix I I I)

. A trial was not conducted in NAFTA growing zo ne 3 as

requested, but a trial was conducted in zone 14 , which is not requested by the US. The trials

were conducted in accordance with the proposed DuPont GAP, except the seed treatment rates

were in excess (1.5 X) of the proposed use. Also, only one trial was supplied in support of a soil

at

- planting treatment. Bridging studies indicated similar residue levels from foliar applications

with the OD and SE formulations. S eed treatment plus foliar treatment yielded higher residues

than foliar treatment only (total application rate 0.4 lb ai/acre). Overall, the trials will support a

tolerance for the tuberous and corm subgroup 1C.

Metabolites were absent.

The potato seed t reatment portion of the trials was conducted at 13.5 g ai /100 kg seed, or 0.013

lb ai /cwt. This is consistent with the proposed label, which specifies a maximum of 0.01 lb ai /

cwt. The actual field trial rate based on amount ai per acre was 0.268 lb ai /a cre, compared with a

label maximum rate of 0.176 lb ai /acre.

Bulb Vegetables (Group 3

- 07)

IR

- 4 has conducted a dry bulb and green onion magnitude and decline of cyantraniliprole

residues field study for DuPont Crop Protection in the 2009 and 2010 growin g seasons. Thirteen

MOR trials and two decline trials were conducted. All trials included a treatment plot that

received three late season foliar applications of Cyantraniliprole 100 g/L OD. Commodity

samplings were made within the window of commercial m aturity for these plots, after the third

application. One decline trial included a treatment plot that received two soil applications

(dripline) of Cyantraniliprole 200 g/L SC at 0.2 lb ai /acre/application

. Commodity samplings

were made within the window of commercial maturity for these plots, after the second

application. E ach treated plot applied a seasonal maximum of approximately 0.402 lb

ai /A/season (450 g ai /ha/season). There were adjuvants added to the spray mixtures.

Samples were analyzed by DuPont Method 15736, Revision No. 1. Samples were extracted with

acetonitrile/water (9/1, v/v) and analyzed by LC/MS

- MS. The LOQ was 0.010 ppm for each

analyte determined. Untreated control samples spiked w ith 0.010

– 5.0 mg/kg cyantraniliprole

and metabolites were analyzed concurrently with the treated samples to verify method

performance. Recoveries for all analyte/matrix combinations ranged from 71

– 118%.The

chromatograms of control samples of dry bulb and g reen onions are free from interferences.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 62 of 193 Recovery values were not corrected for control residues, since no residues were detected at

levels greater than the LOQ of 0.01 ppm in any control sample.

Table 31 . Summary of Statistics for Dry Bulb Onions for 3 Foliar Applications.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A

(g ai /ha) PHI/DAA c

(days)

DuPont

27436 Dry bulb

Onions 100 g/L OD Foliar 3 0.40

- 0.42

(443

- 474) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 20 20 20 20 20 20 20

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.029 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.027 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.014 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.015

-- -- -- -- -- --

SDEV 0.006

-- -- -- -- -- --



### Table 32 . Summary of Statistics for Green Onions for 3 Foliar Applications.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A

(g ai /ha) PHI/DAA c

(days)

DuPont

27436 Green

Onions 100 g/L OD Foliar 3 0.40

- 0.42

(452

- 465) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 10 10 10 10 10 10 10

Min 0.350 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.100 0.016 0.010 0.010 0.010 0.012 0.086

HAFT 4.100 0.016 0.010 0.010 0.010 0.012 0.085

Median 1.300 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.602 0.011

-- -- -- 0.010 0.028

SDEV 1.481 0.003

-- -- -- 0.001 0.031



### Table 33

. Summary of Statistics for Green Onions for Drip Line Soil Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A

(g ai /ha) PHI/DAA c

(days)

DuPont

27436 Green

Onions 200 g/L SC Drip Line

Soil 2 0.41

(454) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.034 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.035 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.035 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.035 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 63 of 193 SDEV

-- -- -- -- -- -- --

a EP = End

- use product

b n = Number of individual s amples ; Min. = Minimum individual sample residue; Max. = Maximum individual sample residue ; HAFT = Highest

average residue from one field trial; Median = Median of average s ample residues; Mean = Mean of average s ample residues

SDEV. = Standard deviation of mean

c DAA = Days after last application



### Residue decline studies were conducted with dry onions and with green onions. All residues

were non

- detectable in the dry onion study. Samples of green onion were taken 1, 3, 7, and 1 3

days after the last of 3 foliar applications of the OD formulation at a rate of 0.134

- 0.136 lb

ai /acre/application. The calculated half

- life was 2.8 days.

Conclusions

.

The Canada/US field onion field trial results were generated using an adequate data collection

method. Adequate storage stability data are av ai lable on diverse crop types. The number and

geographic distribution of trials are generally in accordance with OCSPP harmonized test

guideline 860.1500 (Appendix I II)

. T he 10 dry onion bul b trials exceed the requirement for 6

trials, and all relevant regions are represented. The 5 green onion trials exceed the requirement

for 3 trials. Although only one trial was conducted in region 10, where 2 trials are required,

additional trials were conducted in regions (2 and 5) which are not required. The trials were

conducted in accordance with the proposed DuPont GAP for foliar application of the OD

formulation. Also, only one trial was supplied in support of the soil use of the SC formulation.

In this trial location, the O D ( foliar) and SC formulations (soil drip line) were tested side

- by

side. The residues were greater (120X) with the foliar application. Overall, the trials will

support tolerances for the bulb onion subgroup (3

- 07A) and the green onion subgroup (3

- 07B).

Metabolites were absent, except from 3 foliar treatments of green onions, where IN

- MYX98 and

IN

- N7B69 were each 1

– 2% of the cyantraniliprole concentration.

Leafy vegetables (except Brassica vegetables) (Group 4)

Leafy vegetables (head/leaf lettuce, ce lery, and spinach) were treated with with cyantraniliprole

(OD, SC, SE formulations). Twelve trials were conducted for head lettuce, 12 trials for leaf

lettuce, 11 trials for celery, and 10 trials for spinach. Treatments included three foliar

applications with an OD formulation; one soil treatment with an SC formulation followed by two

foliar applications with an OD formulation; three foliar applications with an SE formulation; two

drip irrigation applications with an SC formulation followed by one foliar application with an

OD formulation. The three foliar applications with the OD formulation were conducted on a plot

at every trial site. In all cases the total application rate was about 0.4 lbs ai /acre. Each foliar

application was about 0.135 lb ai /acr e. Adjuvants were used for all foliar applications.

Commodities were sampled at commercial maturity. For the three foliar application OD

situation, samples were taken 1 day after the second application and 1 day after the third

application.

Samples wer e analyzed by DuPont Method 15736, Revision No. 1. Samples were extracted with

acetonitrile/water (9/1, v/v) and analyzed by LC/MS

- MS. The LOQ was 0.01 ppm for each

analyte. Untreated control samples spiked with 0.010

– 17.0 mg/kg cyantraniliprole and

metab olites were analyzed concurrently with the treated samples to verify method performance.

Recoveries for all analyte/matrix combinations ranged from 68

– 126%, with most recoveriesCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 64 of 193 between 70 and 120%. The LOQ was 0.010 ppm for each analyte determined. The

chromatograms of control samples of leaf lettuce, head lettuce, celery, and spinach were free

from interferences (<0.01 ppm each).

Table 34

. Summary Statistics for Head Lettuce (RAC) from 2 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Head

Lettuce 100 g/L OD Foliar 2 0.262

- 0.276 (294

- 310) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.013 0.010 0.010 0.010 0.010 0.010 0.010

Max 2.900 0.012 0.010 0.015 0.010 0.017 0.010

HAFT 2.500 0.010 0.010 0.010 0.010 0.015 0.010

Median 0.363 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.747 0.010

-- 0.010

-- 0.011

--

SDEV 0.956 0.001

-- 0.001

-- 0.002

--



### Table 35 . Summary Statistics for Head Lettuce (without wrapper leaf) from 2 Foliar Treatments

.

Study No. Crop EP a Appl.

Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Head Lettuce

(for

consumption) 100 g/L

OD Foliar 2 0.262

- 0.276 (294

- 310) 1DAA2

Statisticb DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.210 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.210 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.087

-- -- -- -- -- --

SDEV 0.096

-- -- -- -- -- --



### Table 36 . Summary Statistics for Leaf Lettuce (RAC) from 2 Foliar Treatments

.

Study No. Crop EPa Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAAc

(days)

DuPont

25644 Leaf Lettuce 100 g/L

OD Foliar 2 0.262

- 0.276 (294

- 310) 1DAA2

Statisticb DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.420 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.900 0.018 0.010 0.010 0.037 0.028 0.010

HAFT 4.350 0.016 0.010 0.010 0.035 0.027 0.010

Median 2.900 0.012 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 65 of 193 Mean 2.746 0.012

-- -- 0.016 0.011

--

SDEV 1.688 0.002

-- -- 0.010 0.003

--



### Table 37 . Summary Statistics for Celery (RAC) from 2 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Celery 100 g/L OD Foliar 2 0.262

- 0.276 (294

- 310) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.68 0.010 0.010 0.010 0.010 0.010 0.010

Max 5. 7 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 5.5 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.6 0.010 0.010 0.010 0.010 0.010 0.010

Mean 2.55

-- -- -- -- -- --

SDEV 2.14

-- -- -- -- -- --



### Table 38 . Summary Statistics for Celery (for consumption

– no leaves ) from 2 Foliar Treatments

.

Study No. Crop EPa Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAAc

(days)

DuPont

25644 Celery for

consumption 100 g/L

OD Foliar 2 0.262

- 0.276 (294

- 310) 1DAA2

Statisticb DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.40 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.4 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 4.0 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.73 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.71

-- -- -- -- -- --

SDEV 1.99

-- -- -- -- -- --



### Table 3 9 . Summary Statistics for Spinach (RAC) from 2 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Spinach 100 g/L OD Foliar 2 0.262

- 0.276 (294

- 310) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 3.100 0.020 0.010 0.010 0.010 0.071 0.010

Max 14.000 0.066 0.010 0.010 0.010 0.330 0.010

HAFT 13.500 0.066 0.010 0.010 0.010 0.315 0.010

Median 6.050 0.043 0.010 0.010 0.010 0.240 0.010

Mean 7.225 0.043

-- -- -- 0.220

--

SDEV 4.491 0.017

-- -- -- 0.101

--Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 66 of 193 Table 40 . Summary Statistics for Head Lettuce (RAC) from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Head

Lettuce 100 g/L OD Foliar 3 0.392

- 0.413 (440

- 464) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 24 24 24 24 24 24 24

Min 0.015 0.010 0.010 0.010 0.010 0.010 0.010

Max 2.900 0.021 0.010 0.010 0.010 0.023 0.010

HAFT 2.70 0.018 0.010 0.010 0.010 0.022 0.010

Median 0.695 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.955 0.011

-- -- -- 0.011

--

SDEV 0.855 0.003

-- -- -- 0.003

--



### Table 41 . Summary Statistics for Head Lettuce (for consumption) from 3 Foliar Treatments

Study No. Crop EP a Appl.

Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Head Lettuce

(for

consumption) 100 g/L

OD Foliar 3 0.392

- 0.413 (440

- 464) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.600 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.600 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.200

-- -- -- -- -- --

SDEV 0.310

-- -- -- -- -- --



### Table 42 . Summary Statistics for Leaf Lettuce (RAC) from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Leaf

Lettuce 100 g/L OD Foliar 3 0.392

- 0.413 (440

- 464) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 24 24 24 24 24 24 24

Min 0.920 0.010 0.010 0.010 0.010 0.010 0.010

Max 7.400 0.033 0.010 0.010 0.063 0.027 0.010

HAFT 6.80 0.032 0.010 0.010 0.062 0.024 0.010

Median 2.65 0.012 0.010 0.010 0.016 0.010 0.010

Mean 3.11 0.015

-- -- 0.020 0.013

--

SDEV 1.69 0.007

-- -- 0.016 0.005

--Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 67 of 193 Table 43 . Summary Statistics for Celery with Tops (RAC) from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Celery with

Tops 100 g/L OD Foliar 3 0.392

- 0.413 (440

- 464) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 22 22 22 22 22 22 22

Min 0.240 0.010 0.010 0.010 0.010 0.010 0.010

Max 9.500 0.043 0.010 0.010 0.010 0.029 0.010

HAFT 9.100 0.043 0.010 0.010 0.010 0.026 0.010

Median 2.000 0.019 0.010 0.010 0.010 0.010 0.010

Mean 2.783 0.021

-- -- -- 0.012

--

SDEV 2.684 0.012

-- -- -- 0.005

--



### Table 44 . Summary Statistics for Celery (for consumption) from 3 Foliar Treatments

.

Study No. Crop EP a Appl.

Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Celery (for

consumption) 100 g/L

OD Foliar 3 0.392

- 0.413 (440

- 464) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.34 0.010 0.010 0.010 0.010 0.010 0.010

Max 5.4 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 4.8 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.89 0.010 0.010 0.010 0.010 0.010 0.010

Mean 2.03

-- -- -- -- -- --

SDEV 2.91

-- -- -- -- -- --



### Table 45 . Summary Statistics for Spinach from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Spinach 100 g/L OD Foliar 3 0.392

- 0.413

(440

- 464) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 14 14 14 14 14 14 14

Min 3.600 0.021 0.010 0.010 0.010 0.098 0.010

Max 13.000 0.093 0.010 0.010 0.015 0.410 0.012

HAFT 13.0 0.086 0.010 0.010 0.014 0.400 0.011

Median 4.60 0.034 0.010 0.010 0.010 0.225 0.010

Mean 6.24 0.048

-- -- 0.011 0.225 0.010

SDEV 3.34 0.026

-- -- 0.002 0.107 0.001



### Table 46 . Summary Statistics for Head Lettuce (RAC) from Soil + Foliar Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 68 of 193 DuPont

25644 Head

Lettuce 200 g/L SC

+ 100 g/L

OD 1 soil + 2

foliar 3 0.403

- 0.407 (453

- 457) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.017 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Median 0 .017 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0. 017

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 47 . Summary Statistics for Leaf Lettuce (RAC) from Soil + Foliar Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Leaf

Lettuce 200 g/L SC

+ 100 g/L

OD 1 soil + 2

foliar 3 0.403

- 0.407 (453

- 457) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 1.0 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.1 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 1.1 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.1 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.1

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 48 . Summary Statistics for Celery (RAC) from Soil + Foliar Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Celery 200 g/L SC

+ 100 g/L

OD 1 soil + 2

foliar 3 0.403

- 0.407 (453

- 457) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 3.000 0.015 0.010 0.010 0.010 0.010 0.010

Max 4.100 0.019 0.010 0.010 0.010 0.010 0.010

HAFT 3.500 0.017 0.010 0.010 0.010 0.010 0.010

Median 3.500 0.017 0.010 0.010 0.010 0.010 0.010

Mean 3.5

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 4 9

. Summary Statistics for Spinach (RAC) from Soil + Foliar Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

- Spinach 200 g/L SC 1 soil + 2 3 0.403

- 0.407 (453

- 457) 1DAA3Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 69 of 193 25644 + 100 g/L

OD foliar

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 6.700 0.043 0.010 0.010 0.010 0.180 0.010

Max 6.800 0.049 0.010 0.010 0.010 0.190 0.010

HAFT 6.700 0.046 0.010 0.010 0.010 0.180 0.010

Median 6.700 0.046 0.010 0.010 0.010 0.180 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 50 . Summary Statistics for Head Lettuce (RAC) from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Head

Lettuce 100 g/L SE Foliar 3 446

- 466 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.120 0.010 0.010 0.010 0.010 0.010 0.010

Max 2.200 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 2.100 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.665 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.872

-- -- -- -- -- --

SDEV 0.786

-- -- -- -- -- --



### Table 51 . Summary Statistics for Leaf Lettuce (RAC) from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Leaf

Lettuce 100 g/L SE Foliar 3 0.397

- 0.415 (446

- 466) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 2.300 0.010 0.010 0.010 0.012 0.010 0.010

Max 7.700 0.039 0.010 0.010 0.048 0.028 0.010

HAFT 6.800 0.038 0.010 0.010 0.045 0.023 0.010

Median 4.25 0.014 0.010 0.010 0.014 0.014 0.010

Mean 4.43 0.019

-- -- 0.020 0.016

--

SDEV 1.98 0.010

-- -- 0.013 0.006

--



### Table 52

. Summary Statistics for Head Lettuce (RAC) from Soil + Foliar Trial.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Head

Lettuce 200 g/L SC

+ 100 g/L

OD Soil +

Foliar 2 soil drip

1 foliar 0.401

- 0.403 (451

- 453) 1DAA3Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 70 of 193 Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.810 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.000 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.910 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.910 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Tab le 53 . Summary Statistics for Leaf Lettuce (RAC) from Soil + Foliar Trial.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Leaf

Lettuce 200 g/L SC

+ 100 g/L

OD Soil +

Foliar 2 soil drip

1 foliar 0.401

- 0.403 (451

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 1. 6 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.8 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 1.7 0.010 0.010 0.010 0.010 0.010 0.010

Median 1. 7 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.7

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 54 . Summary Statistics for Celery (RAC) from Soil + Foliar Trial.

Study No. Crop EPa Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Celery 200 g/L SC

+ 100 g/L

OD Soil +

Foliar 2 soil drip

1 foliar 0.401

- 0.403 (451

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.98 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.1 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 1.0 0.010 0.010 0.010 0.010 0.010 0.010

Median 1.0 0.010 0.010 0.010 0.010 0.010 0.010

Mean 1.0

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 55 . Summary Statistics for Spinach (RAC) from Soil + Foliar Trial.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25644 Spinach 200 g/L SC

+ 100 g/L

OD Soil +

Foliar 2 soil drip

1 foliar 0.401

- 0.403 (451

- 453) 1DAA3

Statistic DPX

- IN

- IN

- IN

- IN

- IN

- IN

-Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 71 of 193 HGW86 J9Z38 JCZ38 K7H19 MLA84 MYX98 N7B69

n b 2 2 2 2 2 2 2

Min 6.7 0.024 0.010 0.010 0.010 0.078 0.010

Max 6.8 0.025 0.010 0.010 0.010 0.090 0.010

HAFT 6. 8 0.025 0.010 0.010 0.010 0.084 0.010

Median 6.8 0.025 0.010 0.010 0.010 0.084 0.010

Mean 6.8

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --

a EP = End

- use product

. b n = Number of individual specimens; Min. = Minimum individual specimen residue; Max. = Maximum individual

specimen residue; HAFT = Highest average residue from one field trial; Median = Median of average specimen

residues; Mean = Mean of average specimen residues; SDE V. = Standard deviation of mean.

cDAA = Days after last application



### A residue decline study was conducted on leaf lettuce treated two times (2 X 0.136 lb ai /acre)

with a foliar application of the OD formulation. Lettuce samples were collected 0, 1, 3, and 5

days after the second application. The half

- life was 2.4 days.

Conclusions:

The Canada/US field leafy vegetable field trial results were generated using an adequate data

collection method. Adequate storage stability data are av ai lable on diverse crop. The number

and geographic distribution of trials are in accordance with OCSPP harmonized test guideline

860.1500 (Appendix I I I) . Overall, the trials will support a tolerance for the leafy vegetables

crop gr oup (crop group 4, except Brassica vegetables).

Metabolites were generally absent. IN

- MYX98 was found in head lettuce, leaf lettuce, spinach,

and celery from foliar treatments at 1

– 3% of the cyantraniliprole concentration.IN

- J9Z38 was

found in spinac h, leaf lettuce, and head lettuce at 1% of the cyantraniliprole concentration. IN

M LA84 was found in leaf lettuce and spinach at 1% of the cyantranilirprole concentration.

Brassica (Cole) leafy vegetables (Group 5)

Twenty

- five magnitude of residue (MOR), six bridging, and one decline/bridging trials were

conducted. All 2008 trials included two treatment plots that received either two or three late

season foliar applications of Cyantraniliprole 100 g/L OD. The 2008 bridging trials (three trials

tota l) consisted of side

- by

- side plots that compared 1) the maximum use pattern comprised of

late season foliar applications (Cyantraniliprole 100 g/L OD) to 2) the maximum soil application

treatment (Cyantraniliprole 200 g/L SC) scenario at planting in combin ation with two late season

foliar applications (Cyantraniliprole 100 g/L OD). All 2009 trials included one treatment plot

that received three late season foliar applications of Cyantraniliprole 100 g/L OD. Commodity

samplings were made within the window o f commercial maturity for these plots, after the last

(second or third) applications. The 2009 formulation bridging trials (four broccoli trials total)

consisted of side

- by

- side plots that compared 1) the maximum use pattern comprised of late

season folia r applications with Cyantraniliprole 100 g/L OD to 2) the maximum use pattern

comprised of late season foliar applications with Cyantraniliprole 100 g/L SE. Each treated plot

applied a seasonal maximum of approximately 0.402 lb ai /A/season (450 g ai /ha/se ason). There

were adjuvants (methylated seed oil) added to the spray mixtures for all foliar applications.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 72 of 193 DuPont Method 15736, LC

- MS/MS, was used to quantitate residues of cyantraniliprole

( CYANTRANILIPROLE ) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- M YX98, IN

- MLA84,

IN

- J9Z38, and IN

- K7H19) in/on Brassica (cole) leafy vegetables. Untreated control samples

spiked with 0.010

– 25 mg/kg cyantraniliprole and metabolites were analyzed concurrently with

the treated samples to verify method performance. Recover ies for all analyte/matrix

combinations ranged from 67

– 126%, with most recoveries between 70 and 120%. Although a

few of the recoveries are outside of the criteria range, there is sufficient data to validate the

performance of the analytical method. The chromatograms of control samples of

broccoli/cauliflower, cabbage, and mustard greens are free from interferences. Recovery values

were not corrected for control residues, since no residues were detected at levels greater than the

LOQ of 0.01 ppm in any co ntrol sample.

The maximum residues of cyantraniliprole, and IN

- J9Z38 following 3 foliar applications (100

g/L OD) in/on broccoli/cauliflower were 1.1 ppm, and 0.020 ppm, respectively. The maximum

residues of cyantraniliprole, and IN

- J9Z38 following 3 foli ar applications (100 g/L SE) in/on

broccoli/cauliflower were 1.1 ppm, and 0.017 ppm, respectively.

Table 56 . Summary Statistics for Cabbage (RAC) from 2 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Cabbage

(RAC) 100 g/L OD Foliar 2 0.266

- 0.276 (299

- 310) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 0.220 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.820 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.680 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.500 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.488

-- -- -- -- -- --

SDEV 0.232

-- -- -- -- -- --



### Table 57 . Summary Statistics for Cabbage (without wrapper leaf; wowl) from 2 Foliar

Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Cabbage

(wowl) 100 g/L OD Foliar 2 0.266

- 0.276 (299

- 310) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.027 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.019 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.015 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.017

-- -- -- -- -- --

SDEV 0.007

-- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 73 of 193 Table 58 . Summary Statistics for Mustard Greens from 2 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Mustard

Greens 100 g/L OD Foliar 2 0.266

- 0.276 (299

- 310) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 4.200 0.010 0.010 0.010 0.025 0.014 0.010

Max 11.000 0.049 0.010 0.010 0.067 0.050 0.010

HAFT 10.000 0.048 0.010 0.010 0.063 0.047 0.010

Median 4.950 0.032 0.010 0.010 0.053 0.027 0.010

Mean 6.483 0.030

-- -- 0.047 0.030

--

SDEV 2.977 0.017

-- -- 0.018 0.015

--



### Table 5 9 . Summary Statistics for Broccoli/Cauliflower from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Broccoli/

Cauliflower 100 g/L

OD Foliar 3 0.396

- 0.414 (445

- 465) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 20 20 20 20 20 20 20

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.100 0.020 0.010 0.010 0.010 0.010 0.010

HAFT 1.00 0.019 0.010 0.010 0.010 0.010 0.010

Median 0.53 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.47 0.011

-- -- -- -- --

SDEV 0.33 0.003

-- -- -- -- --



### Table 60 . Summary Statistics for Cabbage (with wrapper leaf; wwl) from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Cabbage

(wwl) 100 g/L OD Foliar 3 0.396

- 0.414 (445

- 465) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 22 22 22 22 22 22 22

Min 0.270 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.980 0.015 0.010 0.010 0.010 0.010 0.010

HAFT 0.95 0.014 0.010 0.010 0.010 0.010 0.010

Median 0.56 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.556 0.010

-- -- -- -- --

SDEV 0.221 0.001

-- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 74 of 193 Table 61 . Summary Statistics for Cabbage (without wrapper leaf; wowl) from 3 Foliar

Treatments

.

Study No. Crop EP a Appl. Type No. of Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Cabbage

(wowl) 100 g/L

OD Foliar 3 0.396

- 0.414 (445

- 465) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.097 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.066 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.022 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.033

-- -- -- -- -- --

SDEV 0.033

-- -- -- -- -- --



### Table 62 . Summary Statistics for Mustard Greens from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Mustard

Greens 100 g/L OD Foliar 3 0.396

- 0.414 (445

- 465) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 22 22 22 22 22 22 22

Min 2.200 0.010 0.010 0.010 0.022 0.010 0.010

Max 20.000 0.230 0.110 0.010 0.260 0.089 0.017

HAFT 19.0 0.220 0.110 0.010 0.230 0.085 0.016

Median 6.00 0.035 0.017 0.010 0.070 0.023 0.010

Mean 7.39 0.060 0.027

-- 0.086 0.034 0.011

SDEV 4.77 0.062 0.0283

-- 0.072 0.024 0.002



### Table 63 . Summary Statistics for Broccoli/Cauliflower from Soil + Foliar Treatment.

Study No. Crop EP a Appl.

Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Broccoli/Cauliflower 200 g/L

SC +

100 g/L

OD 1 soil

(shank) +

2 foliar 3 0.402

- 0.405 (451

- 454) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.470 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.490 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.480 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.480 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 75 of 193 Table 64

. Summary Statistics for Cabbage (RAC) from Soil + Foliar Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Cabbage

(RAC) 200 g/L SC

+ 100 g/L

OD 1 soil

(shank) + 2

foliar 3 0.402

- 0.405 (451

- 454) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.400 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.590 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.490 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.490 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 65 . Summary Statistics for Mustard Greens from Soil + Foliar Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Mustard

Greens 200 g/L SC

+ 100 g/L

OD 1 soil

(shank) + 2

foliar 3 0.402

- 0.405 (451

- 454) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 3.000 0.019 0.010 0.010 0.026 0.010 0.010

Max 3.300 0.021 0.010 0.010 0.034 0.010 0.010

HAFT 3.100 0.020 0.010 0.010 0.030 0.010 0.010

Median 3.100 0.020 0.010 0.010 0.030 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 66 . Summary Statistics for Broccoli/Cauliflower from 3 Foliar Bridging Trial s (100 g/L

SE).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25641 Broccoli/

Cauliflower 100 g/L SE Foliar 3 0.393

- 0.401 (442

- 451) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 0.430 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.100 0.017 0.010 0.010 0.010 0.010 0.010

HAFT 1.100 0.017 0.010 0.010 0.010 0.010 0.010

Median 0.700 0.012 0.010 0.010 0.010 0.010 0.010

Mean 0.743 0.013

-- -- -- -- --

SDEV 0.272 0.003

-- -- -- -- --

a EP = End

- use product;Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 76 of 193 b n = Number of individual specimens; Min. = Minimum individual specimen residue; Max. = Maximum individual

specimen residue; HAFT = Highest average residue from one field trial; Median = Median of average specimen

residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of mean

c DAA = Days after last application



### A decline study was conducted for broccoli that received two foliar treatments of the OD

formulation (3X0.13 lb ai /acre) with sampling at 0, 1, 3, and 5 days after t he second application.

The half

- life was calculated at 3.8 days.

Conclusions:

The Canada/US field leafy vegetable field trial results were generated using an adequate data

collection method. Adequate storage stability data are av ai lable on diverse crop . The number

and geographic distribution of trials are in accordance with OCSPP harmonized test guideline

860.1500 (Appendix I II)

. Metabolites IN

- MLA84 and IN

- MY98 were found at low levels (<1

– 2% cyantraniliprole concentration) in mustard greens from foliar treatment. Overall, the trials

will support tolerances for the Brassica vegetables crop subgroups Brassica head and stem (5A)

and Brassic a leafy greens (5B).

Fruiting Vegetables (Group 8)

NAFTA Field Trial s

Sixteen magnitude of residue (MOR), two bridging, and one decline/bridging trials were

conducted in 2008 and 21 MOR trials were conducted in 2009. All trials included a treatment

plot that received three late season foliar applications of Cyantraniliprole 100 g/L OD.

Commodity samplings w ere made within the window of commercial maturity for these plots,

after the second and third applications in 2008 and after the third application in 2009. The

bridging trials consisted of side

- by

- side plots that compared 1) the maximum use pattern

compri sed of late season foliar applications (Cyantraniliprole 100 g/L OD) to 2) the maximum

soil application treatment (Cyantraniliprole 200 g/L SC) scenario in combination with foliar

applications (Cyantraniliprole 100 g/L OD). Each treated plot applied a sea sonal maximum of

approximately 0.4 lb ai /A/season (450 g ai /ha/season). There were adjuvants added to the spray

mixtures.

DuPont Method 15736, LC

- MS/MS, was used to quantitate residues of cyantraniliprole

( CYANTRANILIPROLE ) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84,

IN

- J9Z38, and IN

- K7H19) in/on fruiting vegetables. Untreated control samples spiked with

0.010

– 4.000 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treated

samples to verify method performance. Re coveries for all analyte/matrix combinations ranged

from 68

– 128%, with most recoveries between 70 and 120%. Although some of the recoveries

are outside of the criteria range, there is sufficient data to validate the performance of the

analytical method. The chromatograms of control samples of tomatoes, bell

- peppers, and non

bell peppers were free from interferences. Recovery values were not corrected for control

residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm in any

co ntrol sample.

Table 67 . Summary Statistics for Bell

- Peppers from 2 Foliar Treatments (1DAA2).

Study No. Crop EP a Appl. Type No. of Total Rate PHI/DAA cCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 77 of 193 Appl. lb ai /A (g ai /ha) (days)

DuPont

25643 Bell

Peppers 100 g/L OD Foliar 2 0.264

- 0.275 (297

- 309) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 10 10 10 10 10 10 10

Min 0.031 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.200 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.200 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.130 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.125

-- -- -- -- -- --

SDEV 0.055

-- -- -- -- -- --



### Table 68 . Summary Statistics for Non

- Bell Peppers from 2 Foliar Treatments (1DAA2).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25643 Non

BellPeppers 100 g/L OD Foliar 2 0.264

- 0.275 (297

- 309) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.410 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.400 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.305 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.293

-- -- -- -- -- --

SDEV 0.084

-- -- -- -- -- --



### Table 69 . Summary Statistics for Bell

- Peppers from 3 Foliar Treatments (1DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25643 Bell

Peppers 100 g/L OD Foliar 3 0.394

- 0.418 (443

- 470) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 22 22 22 22 22 22 22

Min 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.280 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.280 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.075 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.119

-- -- -- -- -- --

SDEV 0.086

-- -- -- -- -- --



### Table 70 . Summary Statistics for Non

- Bell Peppers from 3 Foliar Treatments (1DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

- Non

- Bell 100 g/L OD Foliar 3 0.394

- 0.418 (443

- 470) 1DAA3Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 78 of 193 25643 Peppers

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.066 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.470 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.420 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.186

-- -- -- -- -- --

SDEV 0.123

-- -- -- -- -- --



### Table 71 . Summary Statistics for Tomatoes from 3 Foliar Treatments (1DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25643 Tomatoes 100 g/L OD Foliar 3 0.394

- 0.418 (443

- 470) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 40 40 40 40 40 40 40

Min 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.280 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.260 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.080 0.010 0.010 0.010 0.010 0.010 0.010

SDEV 0.099

-- -- -- -- -- --

SDEV 0.053

-- -- -- -- -- --



### Table 72 . Summary Statistics for Bell

- Peppers from Soil + Foliar Treatment (1DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25643 Bell

Peppers 200 g/L SC

+ 100 g/L

OD Drip

Irrigation +

Foliar 3 0.399

- 0.402 (448

- 452) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.088 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.095 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.092 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.092 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 79 of 193 Table 73 . Summary Statistics for Non

- Bell Peppers from Soil + Foliar Treatment (1DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25643 Non

- Bell

Peppers 200 g/L SC

+ 100 g/L

OD Drip

Irrigation +

Foliar 3 0.399

- 0.402 (448

- 452) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.210 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.150 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.175 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.175 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 74 . Summary Statistics for Tomatoes from Soil + Foliar Treatment (1DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25643 Tomatoes 200 g/L SC

+ 100 g/L

OD Drip

Irrigation +

Foliar 3 0.399

- 0.402 (448

- 452) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.044 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.052 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.048 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.048 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --

a EP = End

- use Product

b n = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen

residues; Mean = Mean of average specimen resi dues; Std Dev = Standard deviation of mean.

c DAA = Days After Application.



### A residue decline study was conducted on tomatoes given two foliar applications of an OD

formulation at about 0.13 lb ai /acre/application. Samples were collected at 0, 1, 3, an d 5 days

after the last application. The cyantraniliprole residue varied from 0.054 to 0.041 ppm. While

residues showed a pattern of decline with increasing PHI , the data were not sufficient to allow

calculation of a half

- life.

Conclusions:

The Canada/US field fruiting vegetable field trial results were generated using an adequate data

collection method. Adequate storage stability data are av ai lable on diverse crop types. The

number and geographic distribution of trials are in accordance with OCSPP harmonized test

guideline 860.1500 (Appendix III ) . Overall, the trials will support a tolerance for the fruitingCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 80 of 193 vegetables crop group 8. Metabolites were not quantifiable in any sample

Europe Greenhouse Trials

Fourteen protected trials were conducted on tomato and cherry tomato during 2009 growing

season with cyantraniliprole applied as foliar treatment with an OD formulation at an application

rate of 4x 120 g ai /ha (4 X 0.107 lb ai /acre)

.

Eight comparative tria ls were also conducted with cyantraniliprole applied via drip irrigation

with an SC formulation and at an application rate of 4 x 100 g ai /ha (4 X 0.089 lb ai /acre).

Mature tomatoes were taken between 1 hour and 7 days after treatment. They were analysed f or

cyantraniliprole, IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- K7H19, IN

- MLA84 and IN

- J9Z38 by

validated method 1187A with a LOQ of 0.01 mg/kg for each analyte

.

No residues above the LOQ of 0.01 mg/kg could be detected in any of the corresponding control

samples. T he maximum storage period of deep frozen was 240 days.

Table 75 . Summary of residue data from EU protected trial

Region Commodity Application

rate ( g ai /ha ) Type of

application

and

formulation PHI

(days) Number

of trials Cyantraniliprole Residue

levels (mg/kg) a

Min Max Median

Cyantraniliprole

Indoor Tomato 4 x120 Foliar, OD 1 9 0.043 0.22 0.14

4 x100 Drip, SC 1 5 <0.01 0.01 0.01

Cherry

Tomato 4 x120 Foliar, OD 1 5 0.14 0.62 0.42

4 x100 Drip, SC 1 3 <0.01 0.008 0.01

a Maximum residue level refers to the sample with the highest residue at the target PHI or later



### Fourteen protected pepper trials were conducted with cyantraniliprole applied as foliar treatment

with an OD formulation and at an application rate of 4x 120 g ai /ha (4x 0.107 lb ai /acre).

Fourteen comparative trials were also conducted with cyantraniliprole applied via drip irrig ation

with an SC formulation at an application rate of 4 x 100 g ai /ha (4x 0.89 lb ai / acre ).

Mature peppers were taken between 1 hour and 14 days after treatment. They were analysed for

cyantraniliprole, IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- K7H19, IN

- MLA84 and IN

- J9Z38 by

validated method 1187A with a LOQ of 0.01 mg/kg for each analyte.

No residues above the LOQ of 0.01 mg/kg could be detected in any of the corresponding control

samples. The maximum storage period of deep frozen was 240 days

.

Table 76 : Summary of residue data from EU protected trial s

Region Commodity Application

rate ( g ai /ha ) Type of

application

and

formulation PHI

(days) Number

of trials Residue levels (mg/kg) 1

Min Max Median

Cyantraniliprole

Indoor Pepper 2 4 x120 Foliar, OD 1 14 0.01 1 0.135

4 x100 Drip, SC 1 14 <0.01 0.01 0.01

1 Maximum residue level refers to the sample with the highest residue at the target PHI or later

2 Includes 5 trials with non

- bell pepper (0.086

– 1 ppm).Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 81 of 193 Conclusion:

The European greenhouse results were generated using an adequate data collection method.

Adequate storage stability data are av ai lable on diverse crop types. Greenhouse trials from non

NAFTA locations may be translated to the US. Overall, the trials will support a tolerance for the

fruiting vegetables crop group 8. Metabolites were not determined in the European trials.

Cucurbit Vegetables (Group 9)

Twenty

- five magnitude of residue (MOR), two bridging, and one decline/bridging trials were

conducted. All trials included a treatment plot that received three late season foliar applications

of Cyantraniliprole 100 g/L OD. Commodity samplings were made within the window of

commercial maturity for these plots, after the second and third applications in 2008 and after the

third application in 2009. The bridging trials consisted of side

- by

- side plots that compared 1) the

maximum use pattern comprised of late season foliar applications (Cyantraniliprole 100 g/L OD)

to 2) the maximum soil application treatment (Cyantraniliprole 20 0 g/L SC) scenario in

combination with foliar applications (Cyantraniliprole 100 g/L OD). Each treated plot applied a

seasonal maximum of approximately 0.4 lb ai /A/season (450 g ai /ha/season). There were

adjuvants added to the spray mixtures.

DuPont Met hod 15736, LC

- MS/MS, was used to quantitate residues of cyantraniliprole

( CYANTRANILIPROLE ) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84,

IN

- J9Z38, and IN

- K7H19) in/on cucurbit vegetables (crop group 9). Untreated control samples

spiked with 0.010

– 0.60 mg/kg cyantraniliprole and metabolites were analyzed concurrently with

the treated samples to verify method performance. Recoveries for all analyte/matrix

combinations ranged from 64

– 127%, with most recoveries between 70 and 120%. Although

so me of the recoveries are outside of the criteria range, there is sufficient data to validate the

performance of the analytical method. The chromatograms of control samples of cucumber,

summer squash, and cantaloupes were free from interferences. Recovery v alues were not

corrected for control residues, since no residues were detected at levels greater than the LOQ of

0.01 ppm in any control sample.

Table 77 . Summary Statistics for Cucumbers from 2 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Cucumber 100 g/L

OD Foliar 2 0.262

- 0.281 (293

- 315) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 6 6 6 6 6 6 6

Min 0.032 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.036 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.060

-- -- -- -- -- --

SDEV 0.040

-- -- -- -- -- --



### Table 78. Summary Statistics for Melon Rind from 2 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 82 of 193 DuPont

25642 Melon Rind 100 g/L OD Foliar 2 0.262

- 0.281 (293

- 315) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 10 10 10 10 10 10 10

Min 0.035 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.350 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.300 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.150 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.160

-- -- -- -- -- --

SDEV 0.097

-- -- -- -- -- --



### Table 79 . Summary Statistics for Melon Pulp from 2 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Melon Pulp 100 g/L OD Foliar 2 0.262

- 0.281 (293

- 315) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 10 10 10 10 10 10 10

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 80 . Summary Statistics for Whole Melon from 2 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Whole

Melon 100 g/L OD Foliar 2 0.262

- 0.281 (293

- 315) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 10 10 10 10 10 10 10

Min 0.042 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.138 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.121 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.095 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.091

-- -- -- -- -- --

SDEV 0.029

-- -- -- -- -- --



### Table 81 . Summary Summer Squash from 2 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Summer

Squash 100 g/L OD Foliar 2 0.262

- 0.281 (293

- 315) 1DAA2

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 83 of 193 n 6 6 6 6 6 6 6

Min 0.074 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.140 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.095 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.099

-- -- -- -- -- --

SDEV 0.024

-- -- -- -- -- --



### Table 82 . Summary Statistics for Cucumbers from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Cucumber 100 g/L

OD Foliar 3 0.383

- 0.412 (430

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 20 20 20 20 20 20 20

Min 0.016 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.200 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.043 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.057

-- -- -- -- -- --

SDEV 0.047

-- -- -- -- -- --



### Table 83 . Summary Statistics for Melon Rind from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Melon Rind 100 g/L OD Foliar 3 0.383

- 0.412 (430

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.023 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.540 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.485 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.175 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.184

-- -- -- -- -- --

SDEV 0.129

-- -- -- -- -- --



### Table 84 . Summary Statistics for Melon Pulp from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Melon Pulp 100 g/L OD Foliar 3 0.383

- 0.412 (430

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 84 of 193 Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 85 . Summary Statistics for Whole Melon from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Whole

Melon 100 g/L OD Foliar 3 0.383

- 0.412 (430

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.048 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.202 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.185 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.105 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.110

-- -- -- -- -- --

SDEV 0.043

-- -- -- -- -- --



### Table 86 . Summary Statistics for Summer Squash from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Summer

Squash 100 g/L OD Foliar 3 0.383

- 0.412 (430

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.059 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.061

-- -- -- -- -- --

SDEV 0.030

-- -- -- -- -- --



### Table 87 . Summary Statistics for Cucumber s from Soil + Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Cucumbers 200 g/L SC

+ 100 g/L

OD 2 Soil +

1 Foliar 3 0.402

- 0.403 (452

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.058 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.120 0.010 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 85 of 193 HAFT 0.089 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.089 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 88 . Summary Statistics for Melon Rin d from Soil + Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Melon Rind 200 g/L SC

+ 100 g/L

OD 2 Soil +

1 Foliar 3 0.402

- 0.403 (452

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.039 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.045 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.042 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.042 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 89 . Summary Statistics for Melon Pulp from Soil + Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Melon Pulp 200 g/L SC

+ 100 g/L

OD 2 Soil +

1 Foliar 3 0.402

- 0.403 (452

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 90 . Summary Statistics for Whole Melo n from Soil + Foliar Treatment s

. d

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Whole

Melon d 200 g/L SC

+ 100 g/L

OD 2 Soil +

1 Foliar 3 0.402

- 0.403 (452

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.036 0.010 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 86 of 193 Max 0.037 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.037 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 91 . Summary Statistics for Summer Squash from Soil + Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

25642 Summer

Squash 200 g/L SC

+ 100 g/L

OD 2 Soil +

1 Foliar 3 0.402

- 0.403 (452

- 453) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.029 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.031 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.030 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --

a EP = End

- use Product

b n = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen

residues; Mean = Mean of average specimen residues; Std Dev = Standard deviation of mean

.

c DAA = Days After Application. Samples were collected 1 day after application 2 or 3, as indicated



### . d Values for Melon, Whole Fruit were calculated from residues determined in melon pulp and melon peel.



### A decline study was conducted for cucumber treated with 3 foliar applications of cyantraniliprole

OD formulation, each application about 0.13 lb ai /acre. Cucumber samples were taken 0.1. 3. 5,

and 6 days after the third application. The half

- life was calculated at 6.7 days.

Conclusions:

The Canada/US field cucurbit vegetable field trial results were generated using an adequate data

collection method. Adequate storage stability data are av ai lable on diverse crop types

. The

number and geographic distribution of trials are in accordance with OCSPP harmonized test

guideline 860.1500 (Appendix II I ) . Overall, the trials will support a tolerance for the cucurbit

vegetables crop group 9. Metabolites were not quantifiable in any sample

Citrus fruit (Group 10)

The 26 trials for oranges, grapefruits, and lemons included 23 magnitude of residue trials and

three decline trials (for soil

- band application). All trials included a treatment plot that received

three foliar applica tions of Cyantraniliprole 100 g/L SE at 7 day retreatment intervals. Low

spray volumes (3

– 75 gpa) or dilute spray volumes (150

– 260 gpa) were used

. Commodities

were sampled 1 day after the third application. Three tests included a treatment plot that

received a single soil band application of Cyantraniliprole 200 g/L SC. Commodities were

sampled at 1, 7, and 14 days after the application. Each treated plot received a seasonalCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 87 of 193 maximum 0.382

- 0.413 lb ai /A/season (429

- 463 g ai /ha/season). For each foli ar application,

adjuvant was added to the spray mixture. The crop growth stage at the time of foliar applications

at the trial sites ranged from BBCH 81

- 89 (beginning of fruit coloring to ripe for consumption).

DuPont Method 15736, LC

- MS/MS, was used to q uantitate residues of cyantraniliprole

( CYANTRANILIPROLE ) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84,

IN

- J9Z38, and IN

- K7H19) in/on citrus fruits. Untreated control samples spiked with 0.010

–

0.10 or 1.0 mg/kg cyantraniliprole and metabol ites were analyzed concurrently with the treated

samples to verify method performance. Although recoveries for all analyte/matrix combinations

ranged from 55

– 141%, most of the concurrent recoveries (approximately 97%) fell within the

range of acceptabilit y specified by the protocol (70

– 120%). The chromatograms of control

samples of 15 s, grapefruits and lemons were free from interferences. Recovery values were not

corrected for control residues, since no residues were detected at levels greater than the LOQ of

0.010 ppm in any control sample.

In the following tables, whole commodity results are calculated from determinations of peel and

pulp.

Table 92 . Summary Statistics for Whole Orange from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Whole

Orange 100 g/L SE Foliar 3 0.382

- 0.413 (429

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 26 26 26 26 26 26 26

Min 0.086 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.401 0.011 0.010 0.010 0.010 0.010 0.010

HAFT 0.390 0.011 0.010 0.010 0.010 0.010 0.010

Median 0.200 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.209 0.010

-- -- -- -- --

SDEV 0.096 0.0003

-- -- -- -- --



### Table 93 . Summary Statistics for Orange Peel from 3 Foliar Treatment s

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Orange

Peel 100 g/L SE Foliar 3 0.382

- 0.413 (429

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 26 26 26 26 26 26 26

Min 0.140 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.910 0.014 0.010 0.010 0.010 0.010 0.010

HAFT 0.885 0.012 0.010 0.010 0.010 0.010 0.010

Median 0.420 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.463 0.010

-- -- -- -- --

SDEV 0.214 0.001

-- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 88 of 193 Table 94 . Summary Statistics for Orange Pulp from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Orange

Pulp 100 g/L SE Foliar 3 0.382

- 0.413 (429

- 463) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 26 26 26 26 26 26 26

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.092 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.087 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.039 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.045

-- -- -- -- -- --

SDEV 0.024

-- -- -- -- -- --



### Table 95 . Summary Statistics for Whole Lemon from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Whole

Lemon 100 g/L SE Foliar 3 0.403

- 0.413 (452

- 462) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.111 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.304 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.300 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.198

-- -- -- -- -- --

SDEV 0.053

-- -- -- -- -- --



### Table 96 . Summary Statistics for Lemon Peel from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Lemon Peel 100 g/L SE Foliar 3 0.403

- 0.413 (452

- 462) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.240 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.630 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.625 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.405 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.413

-- -- -- -- -- --

SDEV 0.117

-- -- -- -- -- --



### Table 97 . Summary Statistics for Lemon Pulp from 3 Foliar Treatments

.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 89 of 193 Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Lemon

Pulp 100 g/L SE Foliar 3 0.403

- 0.413 (452

- 462) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.022 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.110 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.110 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.067 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.064

-- -- -- -- -- --

SDEV 0.028

-- -- -- -- -- --



### Table 98 . Summary Statistics for Whole Grapefruit from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Whole

Grapefruit 100 g/L SE Foliar 3 0.398

- 0.412 (446

- 461) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 14 14 14 14 14 14 14

Min 0.069 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.328 0.011 0.010 0.010 0.010 0.010 0.010

HAFT 0.314 0.011 0.010 0.010 0.010 0.010 0.010

Median 0.133 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.158 0.010

-- -- -- -- --

SDEV 0.076 0.0004

-- -- -- -- --



### Table 99 . Summary Statistics for Grapefruit Peel from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Grapefruit

Peel 100 g/L SE Foliar 3 0.398

- 0.412 (446

- 461) 1DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 14 14 14 14 14 14 14

Min 0.180 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.770 0.019 0.010 0.010 0.010 0.010 0.010

HAFT 0.720 0.015 0.010 0.010 0.010 0.010 0.010

Median 0.340 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.386 0.011

-- -- -- -- --

SDEV 0.159 0.002

-- -- -- -- --



### Tab le 100 . Summary Statistics for Grapefrui t Pulp from 3 Foliar Treatments

.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27554 Grapefruit

Pulp 100 g/L SE Foliar 3 0.398

- 0.412 (446

- 461) 1DAA3Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 90 of 193 Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 14 14 14 14 14 14 14

Min 0.012 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.055 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.049 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.029 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.029

-- -- -- -- -- --

SDEV 0.012

-- -- -- -- -- --

a EP = End

- use Product

b n = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen

residues; Mean = Mean of average specimen resi dues; Std Dev = Standard deviation of mean

c DAA = Days After Application.



### Conclusions:

The US field citrus (lemon, grapefruit, orange) field trial results were generated using an

adequate data collection method. Adequate storage stability data are av ai lable on diverse crop

types. The number and geographic distribution of trials are in accordance with OCSPP

harmonized test guideline 860.1500 (Appendix I I I)

. A residue decline trial was not conducted

for the foliar application scenario with citrus. O verall, the t rials will support a tolerance for the

citrus crop group 10. Metabolites were not quantifiable in any sample

Pome Fruit (Group 11)

Twenty

- five magnitude of residue (MOR) trials and one decline trial were conducted. All trials

included a tr eatment plot that received three late season ai rblast foliar applications of

Cyantraniliprole 100 g/L SE. The retreatment interval was 7 days, and the PHI was 3 days.

Either concentrated sprays of 10

– 80 GPA or dilute spray volumes of 100

– 300 GPA w ere

used. Commodities were sampled within the window of commercial maturity for these plots,

after the third application. Each treated plot applied a seasonal maximum of approximately

0.40 lb ai /A/season (45 g ai /ha/season). For each foliar application, adjuvant was added to the

spray mixture. The crop growth stage at the time of foliar applications at the trial sites ranged

from BBCH 75

- 81 (first application), BBCH 81

- 85 (second application), and BBCH 87

- 89 (third

application).

DuPont Method 15736, LC

- M S/MS, was used to quantitate residues of cyantraniliprole

( CYANTRANILIPROLE ) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84,

IN

- J9Z38, and IN

- K7H19) in/on pome fruits. Untreated control samples spiked with 0.010

–

1.00 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treated samples

to verify method performance. Although recoveries for all analyte/matrix combinations ranged

from 61

– 125%, most recoveries were between 70 and 120%. The chromatograms of control

samples of apples and pears were free from interferences. Recovery values were not corrected

for control residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm

in any control sample.

Note that samples were analyzed for both 3 day and 7 day PHIs. The proposed labels are for a 3

day PHI.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 91 of 193 Table 101 . Summary Statistics for Pears from 3 Foliar Treatments (3DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27438 Pears 100 g/L SE Foliar 3 0.398

- 0.411 (446

- 460) 3DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 20 20 20 20 20 20 20

Min 0.074 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.650 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.535 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.185 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.278

-- -- -- -- -- --

SDEV 0.197

-- -- -- -- -- --



### Table 102 . Summary Statistics for Pears fro m 3 Foliar Treatments (6

- 8DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27438 Pears 100 g/L SE Foliar 3 0.398

- 0.411 (446

- 460) 6

- 8DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 20 20 20 20 20 20 20

Min 0.034 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.590 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.560 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.239

-- -- -- -- -- --

SDEV 0.181

-- -- -- -- -- --



### Table 103 . Summary Statistics for Apples from 3 Foliar Treatments (3DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27438 Apples 100 g/L SE Foliar 3 0.398

- 0.411 (446

- 460) 3DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 32 32 32 32 32 32 32

Min 0.046 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.310 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.310 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.155 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.168

-- -- -- -- -- --

SDEV 0.079

-- -- -- -- -- --



### Table 104 . Summary Statistics for Apples from 3 Foliar Treatments (6

- 8DAA3).

Study No. Crop EP a Appl. Type No. of Total Rate PHI/DAA cCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 92 of 193 Appl. lb ai /A (g ai /ha) (days)

DuPont

27438 Apples 100 g/L SE Foliar 3 0.398

- 0.411 (446

- 460) 6

- 8DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 32 32 32 32 32 32 32

Min 0.052 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.310 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.300 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.125 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.142

-- -- -- -- -- --

SDEV 0.073

-- -- -- -- -- --

a EP = End

- use Product

b N = Number of individual specimens; Min = Minimum individual specimen residue; Max = Maximum individual

specimen residue; HAFT = Highest Average residue from one Field Trial; Median = Median of average specimen

residues; Mean = Mean of average specimen re sidues; Std Dev = Standard deviation of mean

c DAA = Days After Application. Samples were collected 3 or 6 day s after application , as indicated. Note that the

proposed label has a 3 day PHI.



### A residue decline study was conducted on apples. Samples were collected before the third foliar

application and at day 0, 1, 3, and 6 after the third treatment. The calculated half

- life was 17

days.

Conclusion

The US field pome fruit (apple, pear) field trial results were generated using an adequate data

collecti on method. Adequate storage stability data are av ai lable on diverse crop types. The

number and geographic distribution of trials are in accordance with OCSPP harmonized test

guideline 860.1500 (Appendix I II) . Overall consistent residue behavior was foun d in pome fruit

data (crop group 11

- 09) from NAFTA regions for application and sampling conducted according

to critical GAP (cGAP) and the specified use pattern, providing data appropriate for setting

tolerances/MRLs. Metabolites were not quantifiable in any sample.

Stone Fruit (Group 12

- 12 )

Twenty

- nine tests for magnitude and/or decline of residue purposes were categorized as follows:

28 magnitude of residue (MOR), and one decline/MOR. All tests included a treatment plot that

received three late season ai rblast foliar applications of Cyantra niliprole 100 g/L SE at about

0.13 lb ai /acre/application with either low volume (13

– 75 gpa) or high volume (100

- 250 gpa).

Commodity samplings were made within the window of commercial maturity for these plots, just

before or after the third applicatio n. Each treated plot applied a seasonal maximum of

approximately 0.402 lb ai /A/season (450 g ai /ha/season). For each foliar application, adjuvant

was added to the spray mixture. The crop growth stage at the time of foliar applications at the

trial sites ranged from BBCH 75

- 89. Note that the proposed label PHI is 3 days.

DuPont Method 15736, LC

- MS/MS, was used to quantitate residues of cyantraniliprole

( CYANTRANILIPROLE ) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84,

IN

- J9Z38, and IN

- K7H19 ) in/on stone fruits. Untreated control samples spiked with 0.010

–

2.000 mg/kg cyantraniliprole and metabolites were analyzed concurrently with the treatedCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 93 of 193 samples to verify method performance. Recoveries for all analyte/matrix combinations ranged

from 73

- 131%, with most recoveries between 70 and 120%. Although some of the recoveries

were outside of the criteria range, there is sufficient data to validate the performance of the

analytical method. The chromatograms of control samples of peaches, plums, and c herries are

free from interferences. Recovery values were not corrected for control residues, since no

residues were detected at levels greater than the LOQ of 0.01 ppm in any control sample.

T able 105 . Summary Statistics for Peaches from 3 Foliar Treatm ents (3DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27437 Peaches 100 g/L SE Foliar 3 0.398

- 0.413 (446

- 463) 3DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 26 26 26 26 26 26 26

Min 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.400 0.025 0.010 0.010 0.010 0.010 0.010

HAFT 0.960 0.024 0.010 0.010 0.010 0.010 0.010

Median 0.340 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.386 0.012

-- -- -- -- --

SDEV 0.224 0.004

-- -- -- -- --



### Table 106 . Summary Statistics for Peaches from 3 Foliar Treatments (7DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27437 Peaches 100 g/L SE Foliar 3 0.398

- 0.413 (446

- 463) 7DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 26 26 26 26 26 26 26

Min 0.100 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.930 0.037 0.010 0.010 0.010 0.010 0.010

HAFT 0.890 0.032 0.010 0.010 0.010 0.010 0.010

Median 0.230 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.313 0.013

-- -- -- -- --

SDEV 0.216 0.007

-- -- -- -- --



### Table 107 . Summary Statistics for Plums from 3 Foliar Treatments (3DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27437 Plums 100 g/L SE Foliar 3 0.397

- 0.413 (445

- 463) 3DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.028 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.300 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.280 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.066 0.010 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 94 of 193 Mean 0.104

-- -- -- -- -- --

SDEV 0.084

-- -- -- -- -- --



### Table 108 . Summary Statistics for Plums from 3 Foliar Treatments (7DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27437 Plums 100 g/L SE Foliar 3 0.397

- 0.413 (445

- 463) 7DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.028 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.300 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.290 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.060 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.097

-- -- -- -- -- --

SDEV 0.082

-- -- -- -- -- --



### Table 1 09 . Summary Statistics for Cherries from 3 Foliar Treatments (3DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27437 Cherries 100 g/L SE Foliar 3 0.388

- 0.415 (434

- 465) 3DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 14 14 14 14 14 14 14

Min 0.290 0.010 0.010 0.010 0.010 0.010 0.010

Max 3.900 0.053 0.010 0.010 0.010 0.011 0.011

HAFT 3.800 0.052 0.010 0.010 0.010 0.011 0.011

Median 0.930 0.027 0.010 0.010 0.010 0.010 0.010

Mean 1.179 0.026

-- -- -- 0.010 0.010

SDEV 1.190 0.015

-- -- -- -- --



### Table 1 10 . Summary Statistics for Cherries from 3 Foliar Treatments (7DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27437 Cherries 100 g/L SE Foliar 3 0.388

- 0.415 (434

- 465) 7DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 14 14 14 14 14 14 14

Min 0.190 0.010 0.010 0.010 0.010 0.010 0.010

Max 3.100 0.061 0.010 0.010 0.010 0.011 0.010

HAFT 2.200 0.052 0.010 0.010 0.010 0.011 0.010

Median 0.790 0.027 0.010 0.010 0.010 0.010 0.010

Mean 0.880 0.027

-- -- -- 0.010

--

SDEV 0.719 0.017

-- -- -- -- --

a EP = End

- use productCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 95 of 193 b n = Number of individual specimens; Min. = Minimum individual specimen residue

Max. = Maximum individual specimen residue; HAFT = Highest avera ge residue from one field trial; Median =

Median of average specimen residues; Mean = Mean of average specimen residues; SDE V. = Standard deviation of

mean. c DAA = Days after application or PHI. Note that the proposed label PHI is 3 days.



### A residue decline study was conducted with peaches. Samples were collec ted before the third

application and on days 0, 1, 3, and 7 after the third foliar application. The calculated half

- life is

12 days.

Conclusion:

The US field stone fruit (peach, plum, cherry) field trial results were generated using an adequate

data col lection method

. Adequate storage stability data are av ai lable on diverse crop types. The

number and geographic distribution of trials are in accordance with OCSPP harmonized test

guideline 860.1500 (Appendix I I I)

. Overall consistent residue behavior wa s found in stone fruits

(crop group 12

- 09) data from NAFTA regions for application and sampling conducted according

to critical GAP (cGAP), providing data appropriate for setting tolerances/MRLs Metabolite IN

MYX98 was found at a maximum concentration of 0.011 ppm in cherries.

Small fruit and berries (Group 13

- 07)

Eight magnitude of residue (MOR) trials and two decline trials were conducted on blueberries.

All trials included a treatment plot that received three foliar applications of Cyantraniliprole 100

g/L SE. Two trials received a seasonal maximum of approxi mately 0.134 lb ai /A/season (150 g

ai /ha/season). The rem ai ning trials received a seasonal maximum of approximately 0.402 lb

ai /A/season (450 g ai /ha/season). The PHI was 3 days. There were adjuvants added to the spray

mixtures. The crop growth stage at the time of foliar applications at the 0.134 lb ai /A/season

trial sites ranged from BBCH 79

- 88. At the rem ai ning sites, the crop growth stage at the time of

the first foliar application ranged from 4% to 80% mature berries; and from 60% to 100% ripe

berries at the last application.

DuPont Method 15736, LC

- MS/MS, was suitable to quantitate residues of cyantraniliprole and

the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84, IN

- J9Z38, and IN

- K7H19)

in/on blueberries. Untreated control samples spike d with 0.010

– 0.100 or 2.0 mg/kg

cyantraniliprole ( CYANTRANILIPROLE ) and metabolites were analyzed concurrently with the

treated samples to verify method performance. Concurrent recoveries for residues of

cyantraniliprole generally fell within the range of acceptability specified by the protocol (70 to

120%). Recoveries for all analytes ranged from 76 to 127%. The chromatograms of control

samples of blueberries are free from interferences. Recovery values were not corrected for

control residues, since no re sidues were detected at levels greater than the LOQ of 0.01 ppm in

any control sample.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 96 of 193 Table 1 11 . Summary Statistics for Blueberries from 3 Foliar Treatments (3DAA3).

Study No. Crop EPa Appl. Type No. of

Appl. Total Rate

lb ai /A(g ai /ha) PHI/DAA c

(days)

DuPont

27971 Blueberries 100 g/L SE Foliar 3 0.134 ( 150 ) 3DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 4 4 4 4 4 4 4

Min 0.430 0.010 0.01 0.01 0.01 0.010 0.01

Max 1.500 0.014 0.01 0.01 0.01 0.011 0.01

HAFT 1.500 0.013 0.01 0.01 0.01 0.011 0.01

Median 1.055 0.011 0.01 0.01 0.01 0.010 0.01

Mean 1.010 0.012

-- -- -- 0.010

--

SDEV 0.571 0.002

-- -- -- 0.001

--



### Table 1 12 . Summary Statistics for Blueberries from 3 Foliar Treatments (2

- 4DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A

(g ai /ha) PHI/DAA c

(days)

AAFC09

-

025R Blueberries 100 g/L SE Foliar 3 0.397

– 0.408

(445

- 458) 2

- 4DAA3

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 16 16 16 16 16 16 16

Min 0.370 0.010 0.01 0.01 0.01 0.010 0.01

Max 2.000 0.019 0.01 0.01 0.01 0.015 0.01

HAFT 1.950 0.017 0.01 0.01 0.01 0.015 0.01

Median 0.650 0.010 0.01 0.01 0.01 0.010 0.01

Mean 0.880 0.012

-- -- -- 0.011

--

SDEV 0.536 0.003

-- -- -- 0.002

--

a EP = End

- use product

b n = Number of individual specimens ; Min. = Minimum individual specimen residue ; Max. = Maximum individual

specimen residue; HAFT = Highest average residue from one field trial ; Median = Median of average specimen

residues; Mean = Mean of average specimen residues ; SDEV. = Standard deviation of mean

c DAA = Days after application



### Two decline trials (one each in Region 1 and Region 5) were conducted for blueberries receiving

3 applications at a total rate of 0.40 lb ai /acre/season. Samples were taken at 0, 2, 7, and 8 days

after the final treatment and at 0.4, 7, and 10 days after the final treatment. The calculated half

lives were 4.2 and 3.8 days.

Conclusion :

The NAFTA field blueberry field trial results were generated using an adequate data collection

method. Adequate storage stability data are av ai lable on diverse crop types

. The number and

geographic distribution of trials are in accordance with OCSPP harmon ized test guideline

860.1500 (Appendix I I I) . Ove rall consistent residue behavio r was found in blueberry data (crop

subgroup 13

- 07B) from NAFTA regions for application and sampling conducted according to

critical GAP (cGAP), providing data appropriate for s etting tolerances/MRLs. Metabolite IN

MYX98 was found at a maximum concentration of 0.01 5 ppm in blueberries

.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 97 of 193 Tree Nuts (Crop Group 14)

Twelve magnitude of residue (MOR) trials were conducted. All trials included a treatment plot

that received three foliar/ ai rblast applications of Cyantraniliprole 100 g/L OD (TRT 1). Two

trials included a treatment plot that received three foliar applications of Cyantraniliprole 100 g/L

SE (TRT 2). One trial included a treatment plot that received one shank or subsu rface side

- dress

application of Cyantraniliprole 200 g/L SC (TRT 3). Commodity samplings were made 5DAA3

(TRTs 1 and 2) or within the window of commercial maturity (TRT 3). Each treated plot

applied a seasonal maximum of approximately 0.402 lb ai /A/sea son (450 g ai /ha/season). There

were adjuvants added to the spray mixtures. The crop growth stage at the time of foliar

applications at the trial sites ranged from BBCH 75

- 97, or hulls splitting to shucks split and dry.

DuPont Method 15736, LC

- MS/MS, wa s used to quantitate residues of cyantraniliprole and the

metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84, IN

- J9Z38, and IN

- K7H19) in/on

tree nuts. Untreated control samples spiked with 0.010

- 6.0 mg/kg cyantraniliprole and

metabolites were analyzed con currently with the treated samples to verify method performance.

Most of the concurrent recoveries fell within the range of acceptability specified by the protocol

(70

– 120%). Recoveries for all analyte/matrix combinations ranged from 69

- 120%. Therefore,

the analytical methods used perform well for the determination of cyantraniliprole and

metabolites in treated crops. The chromatograms of control samples of almond and pecan are

free from interferences. Recovery values were not corrected for control resid ues, since no

residues were detected at levels greater than the LOQ of 0.01 ppm in any control sample.

Table 1 13 . Summary Statistics for Almond Nutmeat from 3 Foliar Treatments (100 g/L OD).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI (days)

DuPont

27446 Almond

Nutmeat 100 g/L OD Foliar 3 0.390

- 0.410 (437

- 459) 5

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.024 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.023 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.013

-- -- -- -- -- --

SDEV 0.005

-- -- -- -- -- --



### Table 1 14 . Summary Statistics for Almond Nutmeat from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27446 Almond

Nutmeat 100 g/L SE Foliar 3 0.404

- 0.416 (453

- 458) 5

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 4 4 4 4 4 4 4

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.019 0.010 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 98 of 193 HAFT 0.018 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.013 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.014

-- -- -- -- -- --

SDEV 0.004

-- -- -- -- -- --



### Table 1 15 . Summary Statistics for Almond Hulls from 3 Foliar Treatments (100 g/L OD).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI (days)

DuPont

27446 Almond

Hulls 100 g/L OD Foliar 3 0.390

- 0.410 (437

- 459) 5

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.780 0.010 0.010 0.010 0.010 0.010 0.010

Max 4.600 0.026 0.010 0.010 0.010 0.015 0.010

HAFT 4.60 0.025 0.010 0.010 0.010 0.014 0.010

Median 1.60 0.013 0.010 0.010 0.010 0.010 0.010

Mean 2.082 0.014

-- -- -- 0.011

--

SDEV 1.447 0.006

-- -- -- 0.002

--



### Table 1 16 . Summary Statistics for Almond Hulls from 3 Foliar Treatments (100 g/L SE).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27446 Almond

Hulls 100 g/L SE Foliar 3 0.390

- 0.410 (437

- 459) 5

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 4 4 4 4 4 4 4

Min 0.780 0.012 0.010 0.010 0.010 0.010 0.010

Max 4.600 0.041 0.010 0.010 0.010 0.010 0.010

HAFT 4.550 0.038 0.010 0.010 0.010 0.010 0.010

Median 1.550 0.026 0.010 0.010 0.010 0.010 0.010

Mean 2.070 0.026

-- -- -- -- --

SDEV 1.370 0.014

-- -- -- -- --



### Table 1 17 . Summary Statistics for Pecan Nutmeat from 3 Foliar Treatments (100 g/L OD).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27446 Pecan

Nutmeat 100 g/L OD Foliar 3 0.40

– 0.42 (445

- 465) 4

- 5

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 12 12 12 12 12 12 12

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 99 of 193 SDEV

-- -- -- -- -- -- --



### Table 1 18 . Summary Statistics for Pecan Nutmeat from Soil Treatment.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27446 Pecan

Nutmeat 200 g/L SC Soil 1 0.41 (462) 57

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.010 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.010 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --

a EP = End

- use product

b n = Number of individual specimens ; Min. = Minimum individual specimen residue

Max. = Maximum individual specimen residue ; HAFT = Highest avera ge residue from one field trial; Median =

Median of average specimen residues; Mean = Mean of average specimen r esidues; SDEV. = Standard deviation of

mean



### Conclusion:

The NAFTA tree nut field trial results were generated using an adequate data collection method

. Adequate storage stability data are av ai lable on diverse crop types

. The number and geographic

distr ibution of trials are in accordance with OCSPP harmonized test guideline 860.1500. Overall

consistent residue behavior was found in almond and pecan data (crop group 14

- 11; Tree Nuts)

from NAFTA regions for application and sampling conducted according to critical GAP (cGAP),

providing data appropriate for setting tolerances/MRLs. Metabolite IN

- MYX98 was found at a

maximum concentration of 0.015 ppm in almond hulls with the OD formulation.

Oilseed (Group 20)

Cottonseed

The 13 cotton trials included sampl ing for magnitude and/or decline of residue purposes: 10

magnitude of residue (MOR), one bridging, and two decline. All included a treatment plot that

received three late season foliar applications of Cyantraniliprole 100 g/L OD. Commodity

samplings were made within the window of commercial maturity for these plots, after the second

and third applications. The bridging trial consisted of side

- by

- side plots that compared 1) the

maximum use pattern comprised of late season foliar applications (Cyantranilip role 100 g/L OD)

to 2) the maximum soil application treatment (Cyantraniliprole 200 g/L SC) scenario in

combination with foliar applications (Cyantraniliprole 100 g/L OD). Each treated plot received a

seasonal maximum of approximately 0.402 lb ai /A/season (450 g ai /ha/season). For each foliar

application, adjuvant was added to the spray mixture. The crop growth stage at the time of foliar

applications at the trial sites ranged from BBCH 78

- 100, and BBCH 00 (at planting) for the soil

application.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 100 of 193 DuPont Method 15736, LC

- MS/MS, was used to quantitate residues of cyantraniliprole (DPX

HGW36) and the metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84, IN

- J9Z38, and

IN

- K7H19) in/on cotton. Untreated control samples spiked with 0.010

– 1.6 mg/kg

cyantraniliprol e and metabolites were analyzed concurrently with the treated samples to verify

method performance. Most of the concurrent recoveries fell within the range of acceptability

specified by the protocol (70

– 120%). Recoveries for all analyte/matrix combinatio ns ranged

from 59

– 128%. Although some of the recoveries were outside of the criteria range, there is

sufficient data to validate the perfor mance of the analytical method. The chromatograms of

control samples of cotton are free from interferences. Recover y values were not corrected for

control residues, since no residues were detected at levels greater than the LOQ of 0.01 ppm in

any control sample.

Table 11 9 . Summary Statistic for Cottonseed from Treatment # 3 (bridging trial).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27565 Cottonseed 100 g/L

OD

200 g/L SC In

- Furrow

Soil +

Foliar 1 + 2 0.398 (446) 8

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.14 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.16 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.15 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.15 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.15

-- -- -- -- -- --

SDEV

-- -- -- -- -- -- --



### Table 1 20

. Summary Statistic for Cottonseed from Treatment # 3 (bridging trial).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27565 Cottonseed 100 g/L

OD Foliar 3 0.398 (446) 8

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 2 2 2 2 2 2 2

Min 0.27 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.32 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.295 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.295 0.010 0.010 0.010 0.010 0.010 0.010

Mean

-- -- -- -- -- -- --

SDEV

-- -- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 101 of 193 Table 1 21 . Summary Statistic for Cottonseed from Treatment # 1.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27565 Cottonseed 100 g/L

OD Foliar 3 0.398

- 0.416 (446

- 466) 6

- 9

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 26 26 26 26 26 26 26

Min 0.011 0.010 0.010 0.010 0.010 0.010 0.010

Max 1.200 0.064 0.010 0.010 0.010 0.010 0.010

HAFT 0.990 0.061 0.010 0.010 0.010 0.010 0.010

Median 0.160 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.212 0.015

-- -- -- -- --

SDEV 0.257 0.014

-- -- -- -- --



### Table 1 22 . Summary Statistic for Cotton Gin Byproduct from Treatment # 1.

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI/DAA c

(days)

DuPont

27565 Cotton

Gin

Byproduct 100 g/L

OD Foliar 3 0.398

- 0.416 (446

- 466) 6

- 9

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 2.600 0.015 0.010 0.010 0.010 0.010 0.010

Max 5.700 0.077 0.010 0.010 0.010 0.027 0.010

HAFT 5.000 0.0695 0.010 0.010 0.010 0.025 0.010

Median 3.150 0.0495 0.010 0.010 0.010 0.0165 0.010

Mean 3.475 0.0469

-- -- -- 0.0173

--

SDEV 1.074 0.0268

-- -- -- 0.006

--

a EP = End

- use product

b n = Number of individual specimens ; Min. = Minimum individual specimen residue

Max. = Maximum individual specimen residue; HAFT = Highest average residue from one field trial ; Median =

Median of average specimen residues; Mean = Mean of average specimen residues; SDEV. = Standard deviation of

mean

c DAA = Days after last application



### Residue decline studies were conducted in region 4 and regions 6. Cottonseed was sample d at 0,

1, 5, and 7 days after the third foliar application. The half

- lives were calculated as 3.7 and 5.2

days.

Conclusion :

The NAFTA cottonseed field trial results were generated using an adequate data collection

method. Adequate storage stability d ata are av ai lable on diverse crop s

. The number and

geographic distribution of trials are in accordance with OCSPP harmonized test guideline

860.1500 (Appendix III )

. Overall consistent residue behavior was found in cotton data from

NAFTA regions for appl ication and sampling conducted according to critical GAP (cGAP),

providing data appropriate for setting tolerances/MRLs. Metabolite IN

- MYX98 was found at aCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 102 of 193 maximum concentration of 0.027 ppm in cotton gin byproducts. This is <1% of the

cyantraniliprole concentration.

Canola and Sunflower

The 26 trials (17 canola and 9 sunflower) that included sampling for magnitude of residue

purposes were categorized as follows: 26 magnitude of residue (MOR) including 5 MOR seed

treatment trials. All trials included a treatment plot that received three late season foliar

applications of Cyantraniliprole 100 g/L OD. For the seed treatment trials a second plot was

planted to seed treated with Cyantraniliprole 625 g/L FS, then received three late

- season foliar

applicat ions of Cyantraniliprole 100 g/L OD. Commodity samplings were made within the

window of commercial maturity for these plots, after the third and fourth applications. Each

treated plot applied a seasonal maximum of approximately 0.402 lb ai /A/season (450 g

ai /ha/season). For each foliar application, adjuvant was added to the spray mixture. The crop

growth stage at the time of foliar applications at the trial sites ranged from BBCH 72

- 93.

DuPont Method 15736, LC

- MS/MS, was used to quantitate residues of c yantraniliprole and the

metabolites (IN

- N7B69, IN

- JCZ38, IN

- MYX98, IN

- MLA84, IN

- J9Z38, and IN

- K7H19) in/on

oilseed. Untreated control samples spiked with 0.010

– 1.0 mg/kg cyantraniliprole and metabolites

were analyzed concurrently with the treated samples t o verify method performance. Recoveries

for all analyte/matrix combinations ranged from 62

– 131%, with the majority of the concurrent

recoveries within the range of acceptability specified by the protocol (70

– 120%). Although some

of the recoveries were out side of the criteria range, there is sufficient data to validate the

performance of the analytical method. The chromatograms of control samples of canola and

sunflower are free from interferences. Recovery values were not corrected for control residues,

si nce no residues were detected at levels greater than the LOQ of 0.01 ppm in any control

sample.

Side

- by

- side trials to compare 3 foliar applications in/on canola versus a seed treatment followed

by 3 foliar applications indicated that maximum residues of cyantraniliprole were 0.132 ppm,

and 0.098 ppm, respectively, when harvested 7 days after the last application.

Data from one of the trial sites (Trial 3, St. Marc

- sur

- Richelieu, QC, Canada, 2009), were not

considered in the statistics (Table 123) since samples of canola seed were harvested 1 day after

the last application instead of 7 days.

Table 1 23 . Summary Statistics for Canola Seeds From Treatment # 1 (Bridging Trial; 7DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27582 Canola

Seeds 100 g/L OD Foliar 3 Foliar 0.400

- 0.406 (448

- 455) 6

- 7

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 0.017 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.340 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.315 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.039 0.010 0.010 0.010 0.010 0.010 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 103 of 193 Mean 0.104

-- -- -- -- -- --

SDEV 0.132

-- -- -- -- -- --



### Table 1 24 . Summary Statistics for Canola Seeds From Treatment # 2 (Bridging Trial; 7DAA4).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27582 Canola

Seeds 625 g/L FS

+ 100 g/L

OD Seed +

Foliar 1 Seed + 3

Foliar 0.070 + 0.333

- 0.335

(78.6 + 373

- 375) 6

- 7

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 8 8 8 8 8 8 8

Min 0.015 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.220 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.200 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.040 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.077

-- -- -- -- -- --

SDEV 0.086

-- -- -- -- -- --



### Table 1 25 . Summary Statistics for Canola Seeds From Treatment # 1 (7DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27582 Canola

Seeds 100 g/L OD Foliar 3 0.396

- 0.409 (444

- 458) 7

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 32 32 32 32 32 32 32

Min 0.016 0.010 10 10 10 10 10

Max 0.650 0.025 0.010 0.010 0.010 0.010 0.010

HAFT 0.610 0.022 0.010 0.010 0.010 0.010 0.010

Median 0.075 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.148 0.012 0.010 0.010 0.010 0.010 0.010

SDEV 0.157 0.003

-- -- -- -- --



### Table 1 26 . Summary Statistics for Sunflower Seeds From Treatment # 1 (7DAA3).

Study No. Crop EP a Appl. Type No. of

Appl. Total Rate

lb ai /A (g ai /ha) PHI

(days)

DuPont

27582 Sunflower

Seeds 100 g/L OD Foliar 3 0.394

- 0.407 (441

- 456) 5

- 7

Statistic b DPX

HGW86 IN

J9Z38 IN

JCZ38 IN

K7H19 IN

MLA84 IN

MYX98 IN

N7B69

n 18 18 18 18 18 18 18

Min 0.026 0.010 0.010 0.010 0.010 0.010 0.010

Max 0.360 0.010 0.010 0.010 0.010 0.010 0.010

HAFT 0.320 0.010 0.010 0.010 0.010 0.010 0.010

Median 0.069 0.010 0.010 0.010 0.010 0.010 0.010

Mean 0.100

-- -- -- -- -- --Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 104 of 193 SDEV 0.088

-- -- -- -- -- --

a EP = End

- use product

b N = Number of individual specimens; Min. = Minimum individual specimen residue; Max. = Maximum individual

specimen residue; HAFT = Highest average residue from one field trial ; Median = Median of average specimen

residues; Mean = Mea n of average specimen residues ; SDEV. = Standard deviation of mean



### Conclusions

The NAFTA canola and sunflower field trial results were generated using an adequate data

collection method. Adequate storage stability data are av ai lable on diverse crop s. The number

and geographic distribution of trials are in accordance with OCSPP harmonized test guideline

860.1500 (Appendix II I )

. Overall consistent residue behavior was found in cotton data from

NAFTA regions for application and sampling conducted acco rding to critical GAP (cGAP),

providing data appropriate for setting tolerances/MRLs. Metabolites were not quantifiable in

any sample.

The canola seed treatment was conducted at 0.07 lb ai /acre. The label sepecifies a maximum

treatment rate of 1 lb ai /c wt seed. Canola is planted at an average rate of 6 lbs canola seed per

acre (OK State University ; Government of Saskatchewan ). This is equivalent to 0.06 lb ai /acre.

Therefore the trials are reflective of the proposed use. Canola seed treatment use res ults may be

translated to sunflower.

860.1520 Processed Food and Feed

Monograph for Cyantraniliprole , Sections B.7. 1.4 through B7. 1.2 (MRIDs 48120044, 48120011, 48120012

, 48120017, 48120018, 48120020, 48120021, 48120023)



### Nature of the residue in proc essed commodities

Note: This is not a guideline requirement in NAFTA countries.

MRID 48120044: Hydrolysis of radiolabelled cyantraniliprole at 1.0 mg ai /L was studied at 90,

100, and 120 C at pH 4, 5, and 6, respectively, using aqueous citrate buffer. The test solutions

were placed in 12

- mL glass vessels and the headspace was minimized. Acetonitrile (1%) was

used as a co

- solvent. Samples were analyzed at zero time, after 20 min. (pH 4 and 6) and 1 h (pH

5) by LSC and HPLC. Identification of parent and t ransformation products was by co

chromatography and the identifications made were confirmed using LC

- MS analysis. The limit

of quantification (LOQ) for both labeled forms was 1% applied radioactivity ( AR )

. Results are

tabulated below.

Table 1 27

. Distribut ion of radioactivity for [CN

14 

### C]

- cyantraniliprole from simulated processing

conditions

Component pH 4 pH 5 pH 6

Control d 90 C Control d 100 C Control d 120 C

Cyantraniliprole (parent) 97.54 90.56 96.73 74.54 96.92 93.58

IN

- J9Z38 0.69 4.40 1.14 11.51 0.92 6.21

IN

- N5M09 ND 1.90 ND 8.41 ND ND

Others a ND 0.70 0.32 3.39 0.47 1.64

Apparatus wash b 0.77 0.84 0.80 0.74 0.74 0.68

Total % recovery c 99.00 98.40 98.99 98.59 98.92 102.11

a : Sum of minor peaks (individually 5% AR).Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 105 of 193 b : Apparatus wash radioactivity not characterized.

c : Recovery of administered radioactivity as determined by LSC analyses of the dosing solution.

d Indicated pH at ambient temperature.

ND = Not detected



### Table 1 28

. Distribution of radioactivity for [PC

14 

### C]

- cyantraniliprole from simulated processing

conditions

Component pH 4 pH 5 pH 6

Control b 90 C Control b 100 C Control b 120 C

Cyantraniliprole 97.00 89.23 94.71 75.12 98.43 91.49

IN

- J9Z38 0.14 5.36 1.05 13.64 0.36 3.71

IN

- F6L99 ND 1.71 ND 5.33 ND 0.12

Others a 0.80 1.70 1.33 4.04 1.26 2.14

Total % recovery 97.94 97.99 97.09 98.12 100.05 97.46

a : Sum of minor peaks (individually 5% AR).

b Indicated pH at ambient temperature.

ND = Not detected



### Cyantraniliprole was susceptible to hydrolysis under conditions representative of pasteurization

and sterilization, resulting in the formation of IN

- J9Z38. Further degradation of cyantraniliprole

occurred under hydrolytic conditions representative of baking, brewing, or boiling at 100 C for

60 min in p H 5 solution, resulting in the formation of IN

- J9Z38, IN

- F6L99, and IN

- N5M09.

Degradation pathway for cyantraniliprole at high temperature hydrolysis conditions



### Magnitude of the Residue

Processing studies were reviewed for potato, spinach, tomatoes, or anges, apple, plums, and

cottonseed. Generall y residues of cyantraniliprole and metabolites IN

- J9Z38, IN

- JCZ38, IN

K7H19, IN

- MLA84, IN

- MYX98, IN

- N7B69, IN

- N5M09, and IN

- F6L99 were determined with

an LOQ of 0.01 ppm for each. All field trials were conduc ted at an application rate of about 0.40

lb ai /A (1X). This yielded quantifiable residues on all RACs, except one potato trial.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 106 of 193 T able 12 9 : Summary of Processing Factors for Cyantraniliprole

RAC Processed Commodity Processing Factor (Median)

Cyantraniliprole IN

- J9Z38

Potato Flakes 0.6 1

Potato waste 0.6 1

Peeled potato 0.6 1

Chips 0.6 1

Wet peel 0.9 4

Culls 1 1

Fries 0.6 1

Unpeeled boiled 0.6 1

Unpeeled microwaved 0.6 1

Spinach Leaves, cooked 0.16 95

Tomato Washed 0.18 1

Peeled 0.08 1

Sundried 3.5 2.7

Canned 0.08 1

Juice 0.09 1

Wet pomace 0.65 1.7

Dry pomace 1.8 3.5

Paste 0.60 4.2

Puree 0.19 1.3

Orange Juice 0.08 1

Wet Pulp 0.20 1

Dry Pulp 0.38 1

Meal 0.39 1

Molasses 0.08 8.9

Marmalade 0.08 1

Oil 6.2 7.5

Canned 0.077 1

Apple Washed 0.57 1

Puree 1.1 1

Canned 0.12 1

Frozen 0.95 1

Juice 0.32 1

Wet pomace 1 1

Dry pomace 2.6 1.1

Applesauce 1.4 35

Plum Dried 1.5 1.4

Cotton

seed Oil

(solvent ext) 0.03 1

Refined oil

(solvent ext) 0.006 1.5

Meal

(solvent ext) 0.04 1

Hull 0.33 0.89

Oil

(cold press) 0.27 0.89

Refined oil

(cold press) 0.004 1.6Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 107 of 193 T able 12 9 : Summary of Processing Factors for Cyantraniliprole

RAC Processed Commodity Processing Factor (Median)

Cyantraniliprole IN

- J9Z38

Meal

(cold press) 0.09 0.88



### Conclusions

.

The processing studies were conducted according to commercial processes with RAC samples

having quantifiable residues of cyantraniliprole, except one of three potato trial samples. The

storage stability studies support the duration of storage of the RACs and processed fractions.

The data indicate that residues of cyantraniprole concentrated in sundried tomato, dry tomato

pomace, orange oil, dry apple pomace, apple puree, and dried plums. The submitted processing

data are adequate for estimating residue l evels in potential livestock feedstuffs and for dietary

exposure analysis. For the latter, residue data were presented for metabolite IN

- J9Z38

.

Tolerance calculations are presented in Appendix II. Processe d commodities regulated in the US

for which to lerances are considered include orange oil and dried plums. Note that a tolerance

consideration is appropriate for wet apple pomace (1X), but not dry.

860.1650 Submittal of Analytical Reference Standards

A n alytical standard for cyantraniliprole is currently not av ai lable in the EPA National Pesticide

Standards Repository (personal communication with Theresa Cole , ACB

, 01 / 02 / 13 ).

Cyantraniliprole (5 g) and the Certificate of Analysis and the MSDS must be supplied. The

reference standard should b e sent to the Analytical Chemistry Lab, which is located at Fort

Meade, Maryland, to the attention of either There sa Cole or Thuy Nguyen at the following

address:

USEPA

National Pesticide Standards Repository/Analytical Chemistry Branch/OPP

701 Mapes R oad

Fort George G. Meade, MD 20755

- 5350

(Note that the m ai l will be returned if the extended zip code is not used.)

860.1850 Confined Accumulation in Rotational Crops

Monograph for Cyantraniliprole

, Section B.7.9.1 (MRIDs 4 8119986 and 48119987 )



### DuPont submitted confined rotational crops studies with cyantraniliprole labeled in the cyano

and pyrazole carbonyl groups

. Two separate studies were submitted. A pilot study that included

soybeans was not conducted under GLP as supplementary information since soybeans are often

rotated in the field

.

MRID 48119986 : In the pilot study, spring wheat, soybeans, and red beets were grown in a

greenhouse. A single treatment of [PC

14 

### C ] at 300 g ai /ha was applied to a sandy loam soil in

separate pots into which seeds of each crop were sown. Samples of wheat gr ai n, chaff and straw,

red beet foliage and roots, and soybean foliage, pods and beans were harvested 25 days and 120Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 108 of 193 days after treatment (DAT)

. TRR (ppm) in various matrices and distributions of residues i n

wheat, red beet , and soybean are tabulated below.

Table 1 30 . Total radioactive residues in food items sampled from crops sown at intervals

following single 300 g ai /ha applications of [ 14 

### C]

- cyantraniliprole

DAT (days) Commodity

Wheat Soybeans Red Beet

Gr ai n Chaff Straw Seed Foliage Pods Root Foliage

25 0.021 0.374 1.149 0.038 0.185 0.094 0.021 0.107

120 0.019 0.201 0.749 0.018 0.127 0.073 0.008 0.039



### Levels of parent and various metabolites (IN

- K7H19, IN

- K5A77, IN

- K5A78, IN

- K5A79, IN

J9Z38, IN

- JCZ38, IN

- JSE76, IN

- MLA84, IN

- N7B69) in wheat gr ai n, soybean seed and red beet

root were all each <0.01 ppm at 25 a nd 125 day PBI’s. Level s of parent and various metabolites

in wheat chaff and straw, soybean pod and foliage, and red beet foliage each ra nged from <0.01

ppm to 0.52 ppm.

MRID 48119987 : The m ai n study was conducted under GLP and the rotational crops included

lettuce, red beet, and wheat. One 450 g a i /ha application of either [CN

14 

### C]

- cyantraniliprole or

[PC

14 

### C]

- cyantraniliprole was made to bare soil. Seeds of spring wheat, lettuce, and red beet

were sown 30 days and 120 days after treatment. After 365 days aging, a further sowing of

spring wheat was made into the soil which had received the 30 days sowing. Lettuce and red beet

sowing were not conducted at 365 days due to low residues levels determined at earlier plant

back intervals. TRR in various matrices and d istributions of residues in wheat gr ai n, beet root,

and lettuce are tabulated in the tables below.

Table 1 31

. Total radioactive residues in food commodities sampled from crops sown at intervals

following single 450 g ai /ha applications of [ 14 

### C]

- cyantraniliprole

RAC Interval

(DAT a ) [CN

- 14C]

- cyantraniliprole [PC

- 14C]

- cyantraniliprole

TRR ( ppm ) TRR ( ppm )

Wheat gr ai n 30 0.056 0.054

120 0.004 0.008

365 0.014 0.018

Lettuce 30 0.114 0.078

120 0.036 0.023

Red beet roots 30 0.021 0.031

120 0.007 0.009

a: DAT Days after soil treatment



### TRR in various matrices and d istributions of residues in wheat forage, hay and straw, and red

beet foliage are tabulated below.

Table 1 32

. Total radioactive residues in feed items sampled from crops sown at intervals

following single 450 g ai /ha applications of [ 14 

### C]

- cyantraniliprole

RAC Interval

(DAT a ) [CN

14 C]

- cyantraniliprole [PC

14 C]

- cyantraniliprole

TRR

ppm TRR

ppm

Wheat forage 30 0.313 0.287

120 0.129 0.103

365 0.128 0.089Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 109 of 193 Wheat hay 30 1.463 1.625

120 0.312 0.449

365 0.561 0.555

Wheat straw 30 0.973 0.966

120 0.347 0.272

365 0.429 0.482

Red beet

foliage 30 0.063 0.145

120 0.013 0.045

a : DAT Days after soil treatment



### For wheat gr ai n, level s of parent ranged from 0.011 to 0.020 ppm at the 30 day PBI, and was

<0.01 ppm at the 365 day PBI. For red beet root, levels of parent were <0.01 ppm at 30 and 120

day PBI. For lettuce, levels of parent ranged from 0.009 to 0.079 ppm at 30 and 120 day PBI.

Levels of metabolites in these edible crop parts were all <0.01 ppm at these PBIs.

Levels of par ent in wheat forage, hay and straw were all above 0.01ppm at all 3 PBIs except

wheat forage (0.006 ppm) at the 365 day PBI. Levels of parent in beet foliage were all <0.01

ppm at 30 and 120 day PBI. Levels of the metabolites were matrix dependent wheat fee d stuffs

and some (IN

- J9Z38, IN

- MLA84, IN

- MYX98 ) were present at >0.01 ppm but most were

predominantly below 0.01 ppm. Levels of metabolites in beet foliage were all <0.01 ppm.

Table 133 : Nature of the residue in various commodities following single 450 g ai/ha (0.40 lb

ai/acre) application of [ 14 

### C] cyantraniliprole to soil

Identificati

on [CN

14 C]

- cyantraniliprole [PC

14 C]

- cyantraniliprole

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

WHEAT GRAIN

30

- DAT 365

- DAT 30

- DAT 365

- DAT

Cyantranili

prole 20.0 0.011 33.0 0.005 36.3 0.020 13.6 0.003

IN

- J9Z38 2.9 0.002 2.8 <0.001 5.1 0.003 ND

IN

- JCZ38 5.7 0.003 ND 0.8 <0.001 ND

IN

- K5A79 ND ND ND 3.4 0.001

IN

- K7H19 ND ND ND 7.2 0.002

IN

MLA84 1.9 0.001 ND 1.2 0.001 ND

IN

- N7B69 ND ND ND 5.2 0.001

BEET ROOTS

30

- DAT 30

- DAT

Cyantranili

prole 21.0 0.005 23.8 0.007

IN

- DBC80 NA 4.1 <0.001

IN

- J9Z38 3.2 <0.001 5.5 0.002

IN

- K5A77 1.2 <0.001 2.2 <0.001

IN

MYX98 ND ND 2.4 0.001

IN

- N7B69 6.3 <0.001 2.9 <0.001

LETTUCE

30 DAT 120 DAT 30 DAT 120 DAT

Cyantranili

prole 68.1 0.079 69.1 0.025 60.0 0.047 39.6 0.009

IN

- J9Z38 4.5 0.005 1.4 0.001 1.2 0.001 ND ND

IN

- JCZ38 3.7 0.005 1.6 0.001 ND ND ND ND

IN

- 4.3 0.005 2.3 0.001 3.5 0.003 2.1 <0.001Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 110 of 193 Table 133 : Nature of the residue in various commodities following single 450 g ai/ha (0.40 lb

ai/acre) application of [ 14 

### C] cyantraniliprole to soil

Identificati

on [CN

14 C]

- cyantraniliprole [PC

14 C]

- cyantraniliprole

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

MLA84

IN

MYX98 0.7 0.001 16.4 0.007 ND ND 9.1 0.003

WHEAT FORAGE

30 DAT 365 DAT 30 DAT 365 DAT

Cyantranili

prole 72.7 0.226 25.2 0.032 71.0 0.205 6.4 0.006

IN

- J9Z38 4.0 0.013 3.9 0.005 3.8 0.011 ND

IN

- JCZ38 1.8 0.006 6.2 0.008 1.6 0.004 ND

IN

- JSE76 ND ND 0.2 0.001 1.2 0.001

IN

HGW87 ND ND 0.5 0.002 3.4 0.003

IN

- K7H19 ND ND 1.4 0.005 1.2 0.001

IN

- K5A79 ND ND ND 2.1 0.002

IN

MYX98 ND ND ND 1.5 0.001

IN

MLA84 3.9 0.012 6.8 0.008 5.0 0.015 ND

IN

- N7B69 ND ND ND 4.4 0.004

WHEAT HAY

30 DAT 365 DAT 30 DAT 365 DAT

Cyantranili

prole 53.4 0.782 40.8 0.230 52.2 0.848 29.5 0.163

IN

- DBC80 NA NA 1.5 0.025 1.5 0.008

IN

HGW87 ND 0.6 0.004 0.3 0.005 ND

IN

- J9Z38 12.3 0.181 12.9 0.073 10.9 0.178 7.1 0.039

IN

- JCZ38 2.3 0.033 3.7 0.021 2.7 0.044 4.0 0.022

IN

- JSE76 ND 1.0 0.006 0.6 0.010 2.6 0.015

IN

- K5A77 ND 0.8 0.004 ND 0.2 0.001

IN

- K5A78 0.1 0.001 0.2 0.001 ND 0.3 0.001

IN

- K5A79 ND 0.7 0.004 ND 0.2 0.001

IN

- K7H19 ND 1.3 0.007 0.9 0.014 10.9 0.060

IN

MLA84 6.1 0.087 5.5 0.031 6.6 0.108 2.9 0.016

IN

MYX98 0.8 0.013 3.3 0.018 ND ND

IN

- N7B69 1.3 0.019 1.6 0.010 0.3 0.005 0.7 0.004

IN

- NBC94 ND 0.3 0.002 ND ND

WHEAT STRAW

30 DAT 365 DAT 30 DAT 365 DATCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 111 of 193 Table 133 : Nature of the residue in various commodities following single 450 g ai/ha (0.40 lb

ai/acre) application of [ 14 

### C] cyantraniliprole to soil

Identificati

on [CN

14 C]

- cyantraniliprole [PC

14 C]

- cyantraniliprole

% TRR mg/kg % TRR mg/kg % TRR mg/kg % TRR mg/kg

Cyantranili

prole 44.8 0.437 33.0 0.141 41.3 0.399 35.1 0.153

IN

- DBC80 NA NA ND 1.8 0.008

IN

HGW87 ND ND 0.6 0.006 ND

IN

- J9Z38 10.9 0.108 8.9 0.038 9.5 0.091 8.1 0.035

IN

- JCZ38 4.3 0.040 1.8 0.007 5.5 0.053 5.5 0.024

IN

- JSE76 1.5 0.013 4.8 0.021 0.8 0.008 3.9 0.017

IN

- K5A77 0.3 0.002 1.2 0.005 0.3 0.003 1.3 0.007

IN

- K5A78 0.2 0.002 ND ND 0.4 0.002

IN

- K5A79 1.6 0.015 1.5 0.007 ND ND

IN

- K7H19 0.3 0.003 0.8 0.003 ND 10.1 0.043

IN

MLA84 5.8 0.056 5.1 0.022 5.5 0.053 4.5 0.019

IN

MYX98 1.2 0.012 ND ND 1.5 0.014 1.6 0.007

IN

- N7B69 ND ND 4.2 0.018 ND ND

IN

- NBC94 ND ND 0.5 0.004 0.1 0.001

GARDEN BEET FOLIAGE

30 DAT 120 DAT 30 DAT 120 DAT

Cyantranili

prole 3.0 0.003 2.1 <0.001 4.1 0.005 0.8 <0.001

IN

- DBC80 NDd ND 1.7 0.002 ND

IN

HGW87 ND ND 0.2 <0.001 ND

IN

- J9Z38 ND ND ND 0.7 <0.001

IN

- JCZ38 1.5 0.001 2.0 <0.001 1.8 0.003 ND

IN

- JSE76 ND ND 1.0 0.001 3.8 0.002

IN

- K5A77 ND ND 0.2 <0.001 ND

IN

- K5A78 ND ND 0.6 <0.001 ND

IN

- K5A79 1.1 0.001 ND 5.0 0.007 ND

IN

- K7H19 0.7 <0.001 ND 3.2 0.006 ND

IN

MLA84 ND ND 1.7 0.002 ND

IN

MYX98 ND ND 0.5 < 0.001 ND

IN

- N7B69 0.7 <0.001 ND 1.4 0.002 ND

IN

- NBC94 ND ND ND 0.7 <0.001Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 112 of 193 Figure 7 : Proposed metabolic pathway for cyantraniliprole in confined rotational crops

Conclusions

. The submitted studies are adequate to satisfy data requirements

.

Cyantraniliprole was the m ai n residue in wheat gr ai n and lettuce, accounting for up to 0.02 and

0.079 ppm, respectively. Metabolite IN

- MYX98 was detected in lettuce at about 10%

- 15%

TRR, but generally metabolites were individually <10% TR R in food items. In soybean seed

and beet root, TRR was low and no compound individually accounted for >0.01 ppm.

Transfer of cyanra niliprole and its metabolites to animal feed items was higher than observed in

the commodities for human consumption. The TRR in animal feeds ranged from 0.09 to 1.62

ppm at a 30 day PBI

. The predominant residue in wheat chaff, forage, hay, and straw and

soybean foliage was parent.

The m ai n metabolite detected in wheat forage was IN

- J9Z38, which accounted for more than

10% o f the TRR but less than 0.05 ppm

.

The m ai n metabolites detected in wheat hay, were IN

- J9Z38, IN

- K7H19 which accounted for

more than 10% TRR and 0.05 ppm , even for 365 days

- long PBI. IN

- MLA84 was quantified

above at concentrations 0.05 ppm , but did not acco unt for more than 6.6 % TRR.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 113 of 193 The m ai n metabolites detected in wheat straw, were IN

- J9Z38, IN

- K7H19, IN

- MLA84, IN

JCZ38 and IN

- JSE76, which accounted for more than 0.05 ppm . Each of these components was

at least three times less abundant than the parent, wh ich represented 22.9 to 44.8% TRR.

The m ai n metabolites detected in soybean foliage, were IN

- MLA84 and IN

- JSE76, which

accounted for more than 10 %TRR. None of these components was quantified above 0.05 ppm

. The m ai n metabolite detected in soybean pods was IN

- JCZ38, which accounted for more than

10 %TRR, but was not quantified above 0.05 ppm

.

IN

- N7B69 glucoside conjugate (0.02

- 0.05 ppm ) was the principal component in red beet foliage,

whereas parent was not quantified in this commodity.

The metabolic pathw ay is similar to that for primary crops. In wheat, lettuce, red beets, and

soybeans the cyano group underwent varying degrees of hydrolysis to amide and carboxylic acid

(IN

- K7H19, IN

- JCZ38, IN

- JSE76, IN

- K5A77, IN

- K5A78, and IN

- K5A79) moieties.

Cyantraniliprole also cyclized and was dehydrated both with and without loss of the N

- methyl

group to form IN

- J9Z38 and IN

- MLA84 (the demethylated metabolite of IN

- J9Z38).

Demethylation, presumably via oxidation of the N

- methyl group to a hydroxymethyl gro up (as in

IN

- MYX98) then oxidation to the carboxylic acid and eventual loss of carbon dioxide was also a

pathway leading to IN

- HGW87, the free amide of parent. Oxidation on cyantraniliprole’s

benzylic carbon gave the hydroxymethyl metabolite IN

- N7B69. Gluc ose conjugation of IN

N7B69 was also observed. Hydroxylated IN

- MLA84 and bis hydroxy cyantraniliprole were also

reported. IN

- DBC80, formed by cleavage of the carboxamide linkage between the pyridine and

phenyl rings, could be formed from parent or a number of metabolites.

The residu e of concern in rotational crop s for tolerance enforcement and risk assessment is

cyantraniliprole ( ROCKS decision memo, D404411, 9/25/2012 )

. Based on the results of the

confined rotational crop studies (see 860.1900 below) , fie ld rotational crop studies are needed for

cyantraniliprole

.

860.1900 Field Accumulation in Rotational Crops

Monograph for Cyantraniliprole

, Section B.7.9.2 (MRID s 48119995 , 48119996

, 48120005 , 48120035, 48519713 )



### DuPont submitted field rotational crop data from five studies conducted in North America

.

MRID 4811999 6 : In two plots (120

- day soybean and oat restart plots), bare soil was treated with

two applications of cyantraniliprole 100 g/L OD in the 2006/2007 growing s eason. In all other

plots, bare soil was treated with three applications of cyantraniliprole 100 g/L OD. The seasonal

rate was 450 g ai /ha/season. Three applications were made at 150 g ai /ha in all but two plots.

Two applications (150 g ai /ha for Applica tion 1, and 300 g ai /ha for Application 2) were made to

the 120

- day soybean and oat restart plots. Due to unfavorable weather conditions, applications 2

and 3 were combined by doubling the amount of test substance. No test substance was applied to

control plots.

Four (rotational) crops were subsequently planted in the treated and u ntreated soil at the

indicated intervals after the last application (plant

- back intervals).

The only residue found on lettuce, root top, root, and oat gr ai n was cyantranilipro le, which

declined to <LOQ (0.01 ppm) at a 120 day PBI for lettuce, at 14 days for oat gr ai n, radish root,Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 114 of 193 radish top, and soybean seed. Significant residues of metabolites IN

- J9Z38, IN

- JCZ38, and IN

MLA894 were found on the livestock feed commodities oa t forage, oat hay, oat straw, soybean

forage, and soybean hay at 14

– 120 day PBIs. The levels are low, 0.01

– 0.06 ppm per

metabolite, and the total contribution per commodity would be no more than 0.1 ppm at 14

– 30

day PBIs.

Table 1 34 : Maximum Res idues of Cyantraniliprole and Metabolites (IN

- J9Z38, IN

- JCZ38, and IN

MLA84) from Rotational Crop Field Trials Following Treatment with Cyantraniliprole (100 g/L OD)

in the United States (NAFTA Region 11) in 2006

- 2007

Crop Part Cyantraniliprole IN

- J9Z38 IN

- JCZ98 IN

- MLA84

Max PBI PBI * Max PBI PBI * Max PBI PBI * Max PBI PBI *

ppm days days ppm days days ppm days days ppm days days

Lettuce Im

mature 0.039 14 120 <0.01 14 14 <0.01 14 14 <0.01 14 14

Mature 0.033 14 120 <0.01 14 14 <0.01 14 14 <0.01 14 14

Oat Gr ai n <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.036 14 120 <0.01 14 14 0.019 30 365 <0.01 14 14

Hay 0.13 14 365 0.035 14 120 0.056 30 365 <0.01 14 14

Straw 0.045 14 365 0.029 14 365 0.029 120 365 <0.01 14 14

Radish Root <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Top 0.046 14 120 <0.01 14 14 <0.01 14 14 <0.01 14 14

Soybean Seed <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.18 14 365 <0.01 14 14 0.025 120 365 0.023 14 30

Hay 0.023 14 365 <0.01 14 14 0.037 30 365 0.012 14 120

* when residues <LOQ



### MRID 4811999 5 : In a second study, bare soil was treated with three applications of

Cyantraniliprole 100 g/L OD, 3 applications at 150 g ai /ha per application, 5

- day retreatment

interval. No test substance was applied to control plots. Four (rotational) crops were

subsequently planted in the treated and untreated soil at plant

- back intervals (PBIs) of 14, 30,

120, and 365 days. In cereals gr ai n, radish root, soy bean seed, spinach leaves, no residue above

0.01 ppm was quantified in any sample from each plant

- back interval, except in gr ai n from oats

sown 14 and 30 DAT, in which up to 0.01 ppm were quantified.

For livestock feed items, in radish tops no single comp onent was quantified above 0.01 ppm

, except cyantraniliprole in tops from plants sown 14 DAT (0.018 ppm ). Low amounts of IN

J9Z38 only were quantified in tops from plants sown 120 DAT. In spring and winter forage, hay

and straw from 14 and 30 days PBI, cy antraniliprole was the m ai n compound. In hay and straw

samples from 14 and 30 days PBI, sum of IN

- J9Z38 and IN

- JCZ38 was similar to the amount of

cyantraniliprole. In 120 days PBI straw sample, IN

- J9Z38 was the only compound quantified

above 0.01 ppm

.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 115 of 193 Table 1 35 : Maximum Residues of Cyantraniliprole and Metabolites (IN

- J9Z38, IN

- JCZ38, and IN

MLA84) from Rotational Crop Field Trials Following Treatment with Cyantraniliprole (100 g/L OD)

in the United States (NAFTA Region 10) in 2006

- 2007.

Crop Part Cyantraniliprole IN

- J9Z38 IN

- JCZ98 IN

- MLA84

Max PBI PBI * Max PBI PBI * Max PBI PBI * Max PBI PBI *

ppm days days ppm days days ppm days days ppm days days

Spinach Im

mature <0.01 30 30 <0.01 14 14 <0.01 14 14 <0.01 14 14

Mature <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Oat Gr ai n 0.010 30 30 <0.01 14 14 <0.01 14 14 <0.01 14 14

Spring

forage 0.018 14 119 <0.01 14 14 <0.01 14 14 <0.01 14 14

Winter

forage 0.022 30 119 <0.01 30 30 0.010 30 30 <0.01 14 14

Hay 0.096 30 119 0.021 30 119 0.048 30 119 <0.01 14 14

Straw 0.073 30 119 0.033 30 365 0.029 30 119 <0.01 14 14

Radish Root <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Top 0.019 14 31 <0.01 14 14 <0.01 14 14 <0.01 14 14

Soybean Seed <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.043 14 119 <0.01 14 14 0.015 31 119 0.015 14 31

Hay 0.100 14 119 0.023 14 31 0.054 14 119 0.062 14 119

* when residues <LOQ



### MRID 481 2003 5 : A study was conducted in Ontario, Canada, during the 2008

– 2009 growing

seasons. Bare soil was treated with three applications of cyantraniliprole 100 g/L OD, total

application 450 g ai /ha. Four (rotational) crops (lettuce, radish, oats, soybean) were subsequently

planted in the treated and untreated soil at the targeted plant

- b ack intervals (PBIs) of 14, 30, 120,

and 365 days.

Regarding commodities for human consumption (cereals gr ai n, radish root, soybean seed, lettuce

leaves), no residue above 0.01 ppm was quantified in any sample from 365 days plant

- back

interval. Cyantraniliprole was the only compound quantified above 0.01 ppm in immature and

m ature leaves of lettuce from 16 and 29 days PBI. No residue was quantified in samples from

366 days PBI, and no samples were analyzed between 29 and 365 day PBIs. Cyantraniliprole and

IN

- J9Z38 were quantified in radish roots from 30 and 120 days PBI. Resid ues of all metabolites

were less than LOQ at the 16

- day plantback interval. No residue was quantified in samples from

366 days PBI. In cereal gr ai ns, no residue above 0.01 ppm was quantified in any sample.

For livestock feed commodities, in oat forage and hay, maximum residues of cyantraniliprole

were 0.011 ppm , and 0.034 ppm , respectively at the 14

- day plantback interval. The only

metabolites detected above the LOQ were IN

- J9Z38 and IN

- JCZ98 in oat hay at 0.011 ppm and

0.012 ppm , respectively at the 14

- da y PBI. In oat straw, residues of the parent compound and

metabolites were at or less than 0.01 ppm at the 14

- day PBI. In tops of radish, cyantraniliprole

was quantified at 0.039 ppm at the 16

- day PBI. Cyantraniliprole was quantified above 0.01 ppm

in samp les from 14, 29 and 121 days PBI. IN

- J9Z38 and INJCZ38 were quantified at lower

levels (ca 0.005 ppm ). In soybean forage and hay, only cyantraniliprole was quantified at the 15

- day PBI with maximum levels of 0.023 ppm , and 0.061 ppm . Levels declined to l ess than 0.01

ppm at 29 days PBI (forage) and 366 days PBI (hay).Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 116 of 193 Table 1 36 : Maximum Residues of Cyantraniliprole and Metabolites (IN

- J9Z38, IN

- JCZ38, IN

MLA84) from Rotational Crop Field Trials Following Treatment with Cyantraniliprole (100 g/L) in

Ca nada (NAFTA Region 5) in 2008

- 2009

Crop Part Cyantraniliprole IN

- J9Z38 IN

- JCZ98 IN

- MLA84

Max PBI PBI * Max PBI PBI * Max PBI PBI * Max PBI PBI *

ppm days days ppm days days ppm days days ppm days days

Lettuce Im

mature 0.026 16 366 <0.01 16 16 <0.01 16 16 <0.01 16 16

Mature 0.018 29 366 <0.01 16 16 <0.01 16 16 <0.01 16 16

Oat Gr ai n <0.01 14 14 <0.01 14 14 <0.01 14 14 <0.01 14 14

Forage 0.011 14 31 <0.01 14 <0.01 <0.01 14 14 <0.01 14 14

Hay 0.034 14 365 0.011 14 31 0.012 14 31 <0.01 14 14

Straw 0.021 14 31 0.016 14 188 <0.01 14 14 <0.01 14 14

Radish Root 0.015 29 121 <0.01 16 16 <0.01 16 16 <0.01 16 16

Top 0.039 16 121 <0.01 16 16 <0.01 16 16 <0.01 16 16

Soybean Seed <0.01 15 15 <0.01 15 15 <0.01 15 15 <0.01 15 15

Forage 0.023 15 29 <0.01 15 15 <0.01 15 15 <0.01 15 15

Hay 0.061 15 366 <0.01 15 15 0.010 15 15 0.010 15 15

* when residues <LOQ



### MRID 48 120035 : A study was conducted in Georgia, USA; Missouri, USA; Ontario, Canada;

California, USA; and Washington, USA in the 2009/2010 growing season. Bare soil was treated

with three broadcast applications of cya ntraniliprole 100 g/L SE, for a total application rate of

450 g ai /ha (0.40 lb ai/acre) . At each trial location, five to twelve (rotational) crops were

subsequently planted at a targeted PBI of 30 days. Crops were turnip, sugar beet, garden beet,

carrot, bean, pea, soybean, field corn, sweet corn, sorghum, rice, wheat, Bermuda grass, alfalfa,

bromegrass, clover, and bluegrass.

The maximum residue of cyantraniliprole was quantified in carrot root (0.011 ppm ), but not in

other root vegetables. No residue o f cyantraniliprole and metabolites (IN

- J9Z38, IN

- JCZ98, IN

MLA84) was detected in seeds (soybean, pea and bean).

For livestock feed items, residues of cyantraniliprole ranged from <0.01 to 0.012 ppm in tops

from root crops and stover from field corn, swee t corn and sorghum, with the maximum residue

observed in field corn stover. The only metabolite quantified in stover was IN

- J9Z38 in field

corn and sweet corn stover at levels up to 0.013 ppm

.

In forage from wheat, alfalfa, bluegrass and soybean, residues of cyantraniliprole, IN

- J9Z38, IN

JCZ38, and IN

- MLA84 ranged from <0.01

- 0.11 ppm , <0.01

- 0.011 ppm , <0.01 ppm , and <0.01

-

0.020 ppm , respectively. The highest residue was observed in wheat forage. In hay from wheat,

alfalfa, bluegrass, bean, pea, and soybean, residues of cyantraniliprole ranged from 0.024 to 0.63

ppm with the highest residue observed in sorghum hay. The residues of I N

- J9Z38, IN

- JCZ38,

and IN

- MLA84 in hay ranged from <0.01

- 0.028 ppm , <0.01

- 0.029 ppm , and <0.01

- 0.069 ppm

, respectively.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 117 of 193 Table 1 37 : Maximum Residue Data from Rotational Crop Field Trials Following Treatment with

Cyantraniliprole (100 g/L SE) in the United States and Canada in 2009.

Commodity PBI

(days) Cyantraniliprole IN

- J9Z38 IN

- JCZ38 IN

- MLA84

( ppm ) ( ppm ) ( ppm ) ( ppm )

Turnip top 30 <0.01 <0.01 <0.01 <0.01

Turnip root 30 <0.01 <0.01 <0.01 <0.01

Sugar beet top 30 <0.01 <0.01 <0.01 <0.01

Sugar beet root 30 <0.01 <0.01 <0.01 <0.01

Garden beet top 30 <0.01 <0.01 <0.01 <0.01

Garden beet root 30 <0.01 <0.01 <0.01 <0.01

Carrot root 30 0.011 <0.01 <0.01 <0.01

Bean forage 30 0.029 <0.01 <0.01 <0.01

Bean hay 30 0.140 0.014 <0.01 0.017

Bean succulent seed 30 <0.01 <0.01 <0.01 <0.01

Bean dry seed 30 <0.01 <0.01 <0.01 <0.01

Bean seed 30 <0.01 <0.01 <0.01 <0.01

Pea forage 30 0.035 <0.01 <0.01 <0.01

Pea hay 30 0.13 0.010 0.016 <0.01

Pea succulent seed 30 <0.01 <0.01 <0.01 <0.01

Pea dry seed 30 <0.01 <0.01 <0.01 <0.01

Pea seed 30 <0.01 <0.01 <0.01 <0.01

Soybean forage 30 0.091 <0.01 <0.01 0.014

Soybean hay 30 0.63 0.012 0.029 0.069

Soybean seed 30 <0.01 <0.01 <0.01 <0.01

Field corn forage 30 <0.01 <0.01 <0.01 <0.01

Field corn stover 30 0.012 0.012 <0.01 <0.01

Sweet corn forage 30 <0.01 <0.01 <0.01 <0.01

Sweet corn stover 30 0.010 0.013 <0.01 <0.01

Sorghum forage 30 0.010 <0.01 <0.01 <0.01

Sorghum stover 30 <0.01 <0.01 <0.01 <0.01

Rice straw 30 <0.01 <0.01 <0.01 <0.01

Wheat forage 30 0.11 <0.01 <0.01 <0.01

Wheat hay 30 0.21 0.017 <0.01 0.012

Wheat straw 30 0.081 0.012 0.015 0.012

Bermuda grass forage 30

– 31 0.011 <0.01 <0.01 <0.01

Bermuda grass hay 30

– 31 0.025 <0.01 0.010 <0.01

Bromegrass forage 30

– 31 0.011 <0.01 <0.01 <0.01

Bromegrass hay 30

– 31 0.024 <0.01 <0.01 <0.01

Alfalfa forage 30

– 31 0.054 <0.01 <0.01 0.020

Alfalfa hay 30

– 31 0.14 0.020 0.011 0.051

Clover forage 30

– 31 <0.01 0.011 <0.01 <0.01

Clover hay 30

– 31 0.034 0.028 0.019 0.012

Bluegrass forage 30 0.092 <0.01 < 0.01 <0.01

Bluegrass hay 30 0.23 0.028 <0.01 <0.01



### MRID 485 19713 : In another study, bare soil was treated with three bare ground broadcast

applications of cyantraniliprole 100 g/L SE with adjuvants added to the spray mixtures. The total

application rate was 450 g ai /ha.Ten (rotational) crops were subsequently planted in the treated

and untreated soil at 30 1 day targeted intervals after the last application (plant

- back intervals).

The crops were turnip, sugar beet, radish, carrot, pea, soybean, alfalfa, clover, peanut, and

strawberries.

No residue of metabolites IN

- K7 H19, IN

- MYX98 or IN

- N7B69 was detected in any sample. IN

-Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 118 of 193 J9Z38 was found in carrot root at 0.01 ppm. For livestock feed commodities, in clover hay,

soybean hay, turnip tops, soybean forage, alfalfa hay, and clover forage, cyantraniliprole was

quantified at 0.046 ppm, 0.19 ppm, 0.020 ppm, 0.039 ppm, 0.021 ppm, 0.023 ppm, respectively.

Metabolites were quantifiable in soybean hay and clover hay.

Table 1 39

. Maximum Residues of Cyantraniliprole and Metabolites (IN

- J9Z38, IN

- JCZ38, and

IN

- MLA84) from Rotational Crop Following Treatment with Cyantraniliprole (100 g/L SE) in

the United States and Canada (NAFTA Region 1, 2, 5, 6, 7, 8, 9, 10, 11, and 14) in 2010.

Commodity PBI

(days) Cyantraniliprole IN

- J9Z38 IN

- JCZ38 IN

- MLA84

ppm ppm ppm ppm

Carrot root 30

- 32 <0.01 0.010 <0.01 <0.01

Radish root 29

- 31 <0.01 <0.01 <0.01 <0.01

Sugar beet root 29

- 33 <0.01 <0.01 <0.01 <0.01

Sugar beet top 29

- 33 <0.01 <0.01 < 0.01 <0.01

Turnip top 30 0.020 <0.01 <0.01 <0.01

Pea forage 30 <0.01 <0.01 <0.01 <0.01

Pea hay 30 <0.01 0.017 <0.01 <0.01

Pea seed 30 <0.01 <0.01 <0.01 <0.01

Soybean forage 29

- 36 0.039 <0.01 <0.01 <0.01

Soybean hay 29

- 36 0.19 0.021 0.026 0.025

Soybean pod 29

- 36 <0.01 <0.01 <0.01 <0.01

Soybean seed 29

- 36 <0.01 <0.01 <0.01 <0.01

Alfalfa forage 29

- 33 <0.01 <0.01 <0.01 <0.01

Alfalfa hay 29

- 33 0.021 <0.01 <0.01 <0.01

Clover forage 29

- 36 0.023 <0.01 0.013 <0.01

Clover hay 29

- 36 0.046 0.013 0.034 0.011

Peanut hay 29

- 35 <0.01 0.015 <0.01 <0.01

Peanut nutmeat 29

- 35 <0.01 <0.01 <0.01 <0.01

Strawberry berry 29

- 31 <0.01 <0.01 <0.01 <0.01



### Conclusions:

Rotational field trials were conducted for numerous livestock feed commodities and for feed and

food root type commodities (including tops)

. Generally no residues were found at a 365 day

PBI, but t he petitioner proposed a 30 day PBI. The trials were conducted with a 30 day PBI and

with cyantranilirprole applied to the soil at an approximate 1X max imum seasonal rate (0.4 lb

ai/acre).

The number and distribution of trials for each commodity meet the requirements of OCSPP

860.1500, with the exception of cereal grain forages/fodders/straws (Crop Group 16). See

Appendix II I . A total of 42 trials ar e suggested, but only 16 were supplied. This is acceptable,

as residues were not found on any grain, and the concern is livestock feed items only. The trials

were widely distributed across corn (field and sweet), wheat, oats, sorghum, and rice.

The tr ials are adequate to establish inadvertent tolerances for crop sub group 1A (based on radish

root) , crop group 2 (based on radish top) , crop group 7 (based on soybean hay), crop group 16

(based on wheat hay), crop group 17 (based on grass hay), crop group 1 8 (based on alfalfa hay)

, and peanut hay . In such cases, the PBI is 30 days. See Appendix I I for details.

860.1550 Proposed TolerancesCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 119 of 193 DuPont has proposed that the residue of concern for tolerance expression in crop commodities is

cyantraniliprole , that the residue of concern for tolerance expression in livestock commodities is

cyantraniliprole , and that the residue of concern for tolerance expression in processed plant

commodities is the sum of cyantarniliprole and metabolite IN

- J9Z38. HED has co ncluded

(ROCKS decision memo, D404411, 9/25/ 2012) that the residue of concern for tolerance

enforcement in plant commodities, processed plant commodities, and livestock commodities is

cyantraniliprole.

The tolerances proposed are listed in Table 140 along with the tolerance levels recommended by

HED

. The Organization for Economic Cooperation and Development (OECD) tolerance

statistical calculation procedures w ere utilized for determining appropriate tolerance levels for

raw agricultural crop commodi ties , both primary crop and rotational crop ; see Appendix II for

tolerance calculations.

Adequate field trial data are av ai lable to support the proposed uses on Brassica (cole) Leafy

Vegetables (Group 5), Bulb Vegetables (Group 3

- 07), Bushberries (Grou p 13

- 07B), Oilseed

(Group 20), Citrus Fruit (Group 10

- 10), Cucurbit Vegetables (Group 9), Fruiting Vegetables

(Group 8

- 10), Leafy Vegetables (non

- Brassica ) (Group 4), Pome Fruit (Group 11

- 10), Stone

Fruit (Group 12

- 12 ), Tree Nuts (Group 14), and Tuberous and Corm Vegetables (Subgroup 1C).

In some cases the proposed tolerance value (see Table 140 and Appendix II) needs to be

changed, based on the application of the OECD statistical calculation procedure to the validated

field trial data.

Seed treatment of canola indicates that seed treatment plus foliar applications yields residues less

than those from foliar application only, where the total seasonal rate is 0.40 lb ai/acre. This is

supplemented by numerous commodity trials where at

- plant treatment pl us foliar applications do

not exceed residues from foliar treatments only, where the total seasonal rate is 0.40 lb ai/acre.

However, this situation does not apply to root and tuber crops where seed treatment plus foliar

application leads to higher resid ues than foliar treatment only, where the total seasonal rate in

each case is 0.40 lb ai/acre. The canola seed treatment data may be extrapolated to seed treatment

for sunflower seed and mustard seed under the same treatement rates.

There are no data to s upport use in Food/Feed Handling Establishments.

The proposed tolerances for almond hulls, Brassica leafy vegetables head and stem (crop

subgroup 5A), cattle liver, citrus raw peel, cucurbit vegetables (crop group 9), leafy vegetables

except Brassica (cro p group 4) , oilseeds (crop group 20), pome fruits (crop group 11

- 10), potato

wet peel, stone fruits except cherry (crop group 20), and tree nuts (crop group 14), need to be

modified. The recommended primary plant commodity tolerances are derived from use of the

Organization of Economic Cooperation and Development (OECD) statistical calculation

procedure. With the exception of the proposed tolerance for leaves of root and tuber vegetables,

t he proposed tolerances for inadvertent residues, with a PBI of 30 d ays, need to be revised to

reflect the recommended tolerances based on the use of the OECD statistical calculation

procedure. These calculation procedures explain the differences observed in proposed and

recommended tolerances. The calculations are detail ed in Appendix II

. . Also, for some crop

groups, more than one tolerance was proposed for various components of the group, for example,

crop group 6 (forage, fodder, and straw of cereal grains). Only one tolerance is possible per

group, and the tolerance is based on the member commodity with the highest residue levels.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 120 of 193 The proposed tolerances for processed commodities are not appropriate for citrus raw peel and

potato wet peel. The processing studies do not show a concentration of residue in these

commodities relative to the RAC. The proposed tolerance for citrus oil ne eds to be lowered from

6 ppm to 2.4 ppm , consistent with the results from application of the median processing factor to

the HAFT from field trials (orange).

The proposed tolerances for livestock commodities are acceptable, except the proposal s for live r

of cattle and hog and sheep and goat and horse , hog fat, hot meat , hog meat byproducts, and milk

fat

. Evaluation of the feeding study and use of the maximum reasonably balance livestock diets

indicate that a tolerance value of 0.01 ppm is appropriate fo r liver , to be covered the meat

byproducts’ tolerance. The dietary exposure s of hogs and poultry indicate no need for tolerances

(no residue anticipated) , and there is no indication of significant concentration of cyantraniliprole

in milk fat relative to milk.

Currently there are no Codex MRLs/tolerances for cyantraniliprole. Therefore, there are no

issues with harmonization of tolerances. Canadian MRLs are being proposed concurrently with

US tolerances and are harmonized for primary crop commodities an d livestock commodities

except fruiting vegetables. For fruiting vegetables, the US has a greenhouse use not registered

in Canada, and this use necessitates a higher tolerance than the proposed Canada MRL. The US

tolerance for fruiting vegetables is harm onized with the European Union. Also, Canada will

establish import tolerances for grapes and olives. The US does not have this request. Canada

does not establish MRLs for livestock only commodities, and therefore there exists disharmony.

Finally, Cana da must set MRLs for poultry commodities at the LOQ of the analytical method

(0.01 ppm). This is in accord with Canadian policy for situations of no residues anticipated in

the livestock commodities where there are feed items with residues. The US is not establishing

tolerance s for poultry commodities as no significant accumulation in poultry tissues or eggs is

expected, i.e., category 40CFR §180.6(a)(3).

The recommended tolerances for plant and livestock commodities are presented in Table 140

.

Table 140. Tolerance Summary for Cyantraniliprole

.

Commodity Proposed Tolerance

(ppm) Recommended /

Harmonized

Tolerance (ppm) Comments; Correct Commodity

Definition

(a) It is recommended that tolerances be established for residues of the insect icide cyantraniliprole ( 3

- b romo

- 1

- (3

-

chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

- pyrazole

- 5

- carboxamide ),

including its metabolites and degradates, in or on the commodities below. Compliance with the tolerance levels

specified bel ow is to be determined by measuring only cyantraniliprole in or on the commodity.

Almond, hulls 30 8 .0 A revised Section F must be

submitted to propose a tolerance

at 8 ppm

Berries and small fruits,

bushberries (crop subgroup 13

-

07 B ) 4 4 .0 Bushberry subgroup 13

- 07BCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 121 of 193 Table 140. Tolerance Summary for Cyantraniliprole

.

Commodity Proposed Tolerance

(ppm) Recommended /

Harmonized

Tolerance (ppm) Comments; Correct Commodity

Definition

Brassica (cole) leafy

vegetables, head and stem

Brassica (crop subgroup 5A) 2 3 .0 Brassica , head and stem,

subgroup 5A

A revised Section F must be

submitted to propose a tolerance

at 3 ppm

Brassica (cole) leafy

vegetables, leafy Brassica

greens (crop subgroup 5B) 30 30 Brassica , leafy greens, subgroup

5 B

Bulb vegetables, onion, bulb

(crop subgroup 3

- 07A) 0.04 0.04 Onion, bulb, subgroup 3

- 07A

Bulb vegetables, onion, green

(crop subgroup 3

- 07B) 8 8 .0 Onion, green, subgroup 3

- 07B

Cattle, fat 0.01 0.01

Cattle, liver 0.04 None Included under meat byproducts

Cattle, meat 0.01 0.01

Cattle, meat byproduct s , except

liver 0.01 0.01 Cattle, meat byproducts

Cherr ies 6 6 .0 Cherry subgroup 12

- 12A

Citrus fruits (crop group 10

- 10) 0. 7 0.7 0 Fruit, citrus, group 10

- 10

Citrus

, oil 4 2.4 A revised Section F must be

submitted to propose a tolerance

at 2.4 ppm

. Citrus, raw peel 0.9 None Covered by citrus tolernace

Cotton, gin byproduct 10 10 Cotton, gin byproducts

Cucurbit vegetables (crop

group 9) 0.3 0.4 0 A revised Section F must be

submitted to propose a tolerance

at 0.4 ppm

. Vegetable, cucurbit, group 9

Fruiting vegetables (crop

group 8

- 10) 2 2 .0 Vegetable, fruiting , group 8

- 10

Goat, fat 0.01 0.01

Goat, liver 0.04 None Included under meat byproducts

Goat, meat 0.01 0.01

Goat, meat byproducts, except

liver 0.01 0.01 Goat, meat byproducts

Hog, fat 0.01 None 40CFR§180(a)(3)

Hog, liver 0.04 None 40CFR§180(a)(3)

Hog, meat 0.01 None 40CFR§180(a)(3)

Hog, meat byproducts, except

liver 0.01 None 40CFR§180(a)(3)

Horse, fat 0.01 0.01

Horse, liver 0.04 None Included under meat byproducts

Horse, meat 0.01 0.01Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 122 of 193 Table 140. Tolerance Summary for Cyantraniliprole

.

Commodity Proposed Tolerance

(ppm) Recommended /

Harmonized

Tolerance (ppm) Comments; Correct Commodity

Definition

Horse, meat byproducts, except

liver 0.01 0.01 Horse, meat byproducts.

Leafy vegetables (except

Brassica vegetables) (crop

group 4) 15 20 A revised Section F must be

submitted to propose a tolerance

at 20 ppm

. Vegetable, leafy, except

Brassica , group 4

Milk 0.01 0.01

Milk fat 0.04 None Covered by milk tolerance

Oilseeds, except cotton

byproduct (crop group 20) 1 1.5 A revised Section F must be

submitted to propose a tolerance

at 1.5 ppm

. Oilseed group 20

Pome fruits (crop group 11

- 10) 0.8 1.5 A revised Section F must be

submitted to propose a tolerance

at 1.5 ppm

. Fruit, pome, group 11

- 10

Potato, wet peel 0.3 None Covered under subgroup 1C

tolerance

Root and tuber vegetables,

tuberous and corm vegetables

(crop subgroup 1C) 0.15 0.15 Vegetable, tuberous and corm,

subgroup 1C

Sheep, fat 0.01 0.01

Sheep, liver 0.04 None Included under meat byproducts

Sheep, meat 0.01 0.01

Sheep, meat byproducts,

except liver 0.01 0.01 Sheep meat byproducts

Stone fruits, except cherries

(crop group 12) 1.5 1.5 Peach subgroup 12

- 12B

0.5 0 Plum subgroup 12

- 12C

Tree nuts, except almond hulls

(crop group 14) 0.06 0.04 A revised Section F must be

submitted to propose a tolerance

at 0.04 ppm

. Nut, tree, group 14

(d) It is recommended that indirect or inadvertent tolerances be established for residues of the insect icide

cyantraniliprole ( 3

- b romo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

- 1 H

pyrazole

- 5

- carboxamide ), including its metabolites and degradates, in or on the commodities below. Compliance

with the tolerance levels specified bel ow is to be determined by measuring only cyantraniliprole in or on the

commodity.

Foliage of legume vegetables

(crop group 7), forage 0.15 None Only one tolerance per crop

group.

Foliage of legume vegetables

(crop group 7), hay 0.6 0. 7 0 A revised Section F must be

submitted to propose a tolerance

at 0. 7 ppm

. Vegetable, foliage of legume,

group 7

Forage, fodder and straw of

cereal gr ai ns (crop group 16),

forage 0.06 None Only one tolerance per crop

group.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 123 of 193 Table 140. Tolerance Summary for Cyantraniliprole

.

Commodity Proposed Tolerance

(ppm) Recommended /

Harmonized

Tolerance (ppm) Comments; Correct Commodity

Definition

Forage, fodder and straw of

cereal gr ai ns (crop group 16),

hay and straw 0.15 0.5 0 A revised Section F must be

submitted to propose a tolerance

at 0.5 ppm

. Gr ai n, cereal, forage, fodder and

straw, group 16

Grass forage, fodder, and hay

(crop group 17), forage 0.06 None Only one tolerance per crop

group.

Grass forage, fodder, and hay

(crop group 17), hay 0.15 0.5 0 A revised Section F must be

submitted to propose a tolerance

at 0.5 ppm

. Grass , forage, fodder and hay

, group 1 7

Leaves of root and tuber

vegetables (human food or

animal feed) (crop group 2) 0.04 0.0 4 Vegetable, leaves of root and

tuber, group 2

Nongrass animal feeds (forage,

fodder, straw, and hay) (crop

group 18), forage 0.06 None Only one tolerance per crop

group.

Nongrass animal feeds (forage,

fodder, straw, and hay) (crop

group 18), hay 0.15 0.2 0 A revised Section F must be

submitted to propose a tolerance

at 0.2 ppm

. Animal feed, nongrass, group 18

Peanut

, hay 0.03 0.01 A revised Section F must be

submitted to propose a tolerance

at 0.01 ppm

. Peanut, hay

Root and tuber vegetables, root

vegetables (crop subgroup 1A) 0.03 0.02 A revised Section F must be

submitted to propose a tolerance

at 0.02 ppm

. Vegetable, root, subgroup 1A



### Attachments:

International Residue Limits

Appendix I

– Summary of Cyantraniliprole and Metabolites

Appendix II

- Tolerance Assessment Calculations

Template Version September 2005Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 124 of 193 

## International Residue Limits

Cyantraniliprole ( 090098; 12/31/2012 )

Summary of US and International Tolerances and Maximum Residue Limits

Residue Definition :

US Canada Mexico 2 

### Codex 3

40 CFR 180.XXX:

Plants: T olerances are established for

residues of the insect icide cyantraniliprole ( 3

-

b romo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

-

1 H

- pyrazole

- 5

- carboxamide ), including its

metabolites and degradates, in or on the

commodities below. Compliance with the

tolerance levels specified below is to be

determined by measuring only

cyantranili prole in or on the commodity.

Livestock: T olerances are established for

residues of the insect icide cyantraniliprole ( 3

-

b romo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

2

- methyl

- 6

- [(methylamino)carbonyl]phenyl]

-

1 H

- pyrazole

- 5

- carboxamide ), including its

metabolite s and degradates, in or on the

commodities below. Compliance with the

tolerance levels specified below is to be

determined by measuring only

cyantraniliprole in or on the commodity. Identical

to US None



### Commodity 1 

### Tolerance (ppm) /Maximum Residue Limit ( ppm )

US Canada Mexico 2 

### Codex 3

Almond, hulls 8 .0 8

Bushberry subgroup 13

- 07B 4 .0 4

Brassica , head and stem, subgroup

5A 3 .0 3

Brassica , leafy greens, subgroup B 30 30

Onion, bulb, subgroup 3

- 07A 0.04 0.04

Onion, green, subgroup 3

- 07B 8 .0 8

Cattle fat 0.01 0.01

Cattle, meat 0.01 0.01

Cattle, meat byproducts 0.01 0.01

Cherry subgroup 12

- 12B 6 .0 6 (cherry)

Fruit, citrus, group 10

- 10 0.7 0.7

Citrus, oil 2.4 2.4

Cotton, gin byproducts 10 10

Vegetable, cucurbit, group 9 0.4 0.4

Vegetable, fruiting, group 8

- 10 2 .0 0.7

Goat, fat 0.01 0.01

Goat, meat 0.01 0.01

Goat, meat byproducts 0.01 0.01

Horse, fat 0.01 0.01

Horse, meat 0.01 0.01

Horse, meat byproducts 0.01 0.01Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 125 of 193 Summary of US and International Tolerances and Maximum Residue Limits

Residue Definition :

US Canada Mexico 2 

### Codex 3

Vegetable, leafy, except Brassica

, group 4 20 20

Milk 0.01 0.01

Oilseed group 20 1.5 1.5

Fruit, pome, group 11

- 10 1.5 1.5

Vegetable, tuberous and corm,

subgroup 1C 0.15 0.15

Sheep, fat 0.01 0.01

Sheep, meat 0.01 0.01

Sheep, meat byproducts 0.01 0.01

Peach subgroup 12

- 12A 1.5 1.5

(stone

fruit

except

cherry) Plum subgroup 12

- 12C 0.5

Nut, tree, group 14 0.04 0.04

Inadvertent Tolerances

Vegetable, foliage of legume, group

7 0. 7 N/A 4

Gr ai n, cereal, forage, fodder and

straw, group 16 0.5 N/A

Grass, forage, fodder and hay, group

17 0.5 N/A

Vegetable, leaves of root and tuber,

group 2 0.0 4 0.0 4

Animal feed, nongrass, group 18 0.2 N/A

Peanut, hay 0.01 N/A

Vegetable, root, subgroup 1A 0.02 0.02

1 Includes only commodities of interest for this action. Tolerance values should be the HED recommendations and

not those proposed by the applicant.

2 Mexico adopts US tolerances and/or Codex MRLs for its export purposes.

3 * = absent at the limit of quan titation; Po = postharvest treatment, such as treatment of stored gr ai ns. PoP =

processed postharvest treated commodity, such as processing of treated stored wheat. (fat) = to be measured on the

fat portion of the sample. MRLs indicated as proposed have n ot been finalized by the CCPR and the CAC.

4 Canada does not establish tolerances for livestock feed commodities. Canada, by law, must include MRLs for

poultry at the LOQ. Also, Canada is establishing import MRLs for olives and grapes as per request of DuPont.

These MRLs are harmonized with the EU.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 126 of 193 APPENDIX I: Structures of Major Cyantraniliprole Metabolites

Common Name Chemical Name Structure

Cyantraniliprole

SYN545377

CYANTRANILIPROLE CAS Name: 3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- methyl

- 6

[(methylamino)carbonyl]phenyl]

- 1 H

pyrazole

- 5

- carboxamide

IN

- MLA84 CAS Name: 2

- [3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1 H

- pyrazol

- 5

- yl]

- 1,4

- dihydro

- 8

- methyl

- 4

- oxo

- 6

- quinazolinecarbonitrile

IN

- N7B69 CAS Name: 3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- (hydroxymethyl)

-

6

- [(methylamino)carbonyl]phenyl]

- 1 H

pyrazole

- 5

- carbonitrile

IN

- MYX98 CAS Name: 3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- N

- [4

- cyano

- 2

- [[(hydroxymethyl)

amino]carbonyl]

- 6

- [(methylphenyl]

- 1 H

pyrazole

- 5

- carboxamide

IN

- J9Z38 CAS Name: 2

- [3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1 H

- pyrazol

- 5

- yl]

- 3,4

- dihydro

3,8

- dimethyl

- 4

- oxo

- 6

- quinazolinecarbonitrile

IN

- NXX69 CAS Name: 2

- [[(4Z)

- 2

- bromo

- 4H

pyrazolo[1,5

- d]pyrido[3,2

- b][1,4]oxazin

- 4

- ylidene]amino]

- 5

- cyano

- N,3

- dimethylbenzamideCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 127 of 193 Common Name Chemical Name Structure

IN

- QKV54 CAS Name: 2

- (3

- Bromo

- 1H

- pyrazol

- 5

- yl)

- 1,4

- dihydro

- 3,8

- dimethyl

- 4

- oxo

- 6

- quinazolinecarbonitrile

IN

- RNU71 CAS Name : 2

- (2

- Bromo

- 4

- oxopyrazolo[1,5

- a]pyrido[3,2

- e]pyrazin

5(4H)

- yl)

- 5

- cyano

- N,3

- dimethylbenzamide

IN

- JSE76 CAS Name : 4

- [[[3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1H

- pyrazol

- 5

- yl]carbonyl]amino]

- 3

- methyl

- 5

- [(methylamino)carbonyl]benzoic acid

IN

- JCZ38 CAS Name : 4

- [[[3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1H

- pyrazol

- 5

- yl]carbonyl]amino]

- N'3',5

- dimethyl

- 1,3

-

benzenedicarboxamide

IN

- K5A78 CAS Name : 2

- [3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1H

- pyrazol

- 5

- yl]

- 3,4

- dihydro

3,8

- dimethyl

- 4

- oxo

- 6

- quinazolinecarboxylic

acid

IN

- K5A77 CAS Name : 2

- [3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1H

- pyrazol

- 5

- yl]

- 3,4

- dihydro

3,8

- dimethyl

- 4

- oxo

- 6

- quinazolinecarboxamide

IN

- K5A79 CAS Name : 3

- (Aminocarbonyl)

- 4

- [[[3

-

bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1H

- pyrazol

5

- yl]carbonyl]amino]

- 5

- methylbenzoic acidCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 128 of 193 Common Name Chemical Name Structure

IN

- PLT97 CAS Name: 2

- [3

- Bromo

- 1

- (3

- chloro

- 2

- pyridinyl)

- 1 H

- pyrazol

- 5

- yl]

- 1,4

- dihydro

- 8

- methyl

- 4

- oxo

- 6

- quinazolinecarboxylic acidCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 129 of 193 

## Appendix II : TOLERANCE ASSESSMENT CALCULATIONS

MRL Calculations for Raw Agricultural Commodities (Primary Crops and

Rotational Crops) and Processed Commodities

Tolerance estimates for primary crops and rotational crops were derived using the field trial data

and the Organization of Economic Cooperation and Development (OECD) calculation

procedure. Det ai ls are provide d below for each crop/crop group or subgroup.

Tolerances for Raw Agricultural Commodities from Primary Crop Field Trials

Tuberous and Corm vegetables (subgroup 1C)

The tolerance estimate for subgroup 1C is 0.15 ppm , based on potato seed treatment plus foliar

treatment.

.

Potato

One seed treatment plus one late season foliar treatment

.

Cyantraniliprole

Potato

NAFTA

Seed treatment FS (13.5 g ai /100 kg seed) + 1 Foliar OD

(0.13

– 0.14 lb ai /acre), 7 day PHI

Total number of data (n) 21

Percentage of censored data 33%

Number of non

- censored data 14

Lowest residue 0.010

Highest residue 0.110

Median residue 0.014

Mean 0.025

Standard deviation (SD) 0.025

Correction factor for censoring (CF) 0.778

Proposed MRL estimate

- Highest residue 0.110

- Mean + 4 SD 0.125

- CF x 3 Mean 0.057

Unrounded MRL 0.125

Rounded MRL 0.15Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 130 of 193 Residues (mg/kg) n

< 0.01 7

0.01 2

0.011 1

0.014 2

0.02 1

0.023 2

0.027 1

0.028 1

0.031 1

0.052 1

0.072 1

0.11 1

Three late season foliar applications

Cyantraniliprole

Potato

USA

3 foliar OD

Total number of data (n) 19

Percentage of censored data 79%

Number of non

- censored data 4

Lowest residue 0.010

Highest residue 0.034

Median residue 0.010

Mean 0.012

Standard deviation (SD) 0.006

Correction factor for censoring (CF) 0.481

Proposed MRL estimate

- Highest residue 0.034

- Mean + 4 SD 0.037

- CF x 3 Mean 0.018

Unrounded MRL 0.037

Rounded MRL 0.04

High uncert ai nty of MRL estimate.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 131 of 193 [High level of censoring]

Residues (mg/kg) n

< 0.01 15

0.011 1

0.018 1

0.02 1

0.034 1

3 foliar SE

Cyantraniliprole

Potato

NAFTA

SE, 3X0.13, 7 d PHI

Total number of data (n) 5

Percentage of censored data 60%

Number of non

- censored data 2

Lowest residue 0.010

Highest residue 0.030

Median residue 0.010

Mean 0.016

Standard deviation (SD) 0.009

Correction factor for censoring (CF) 0.600

Proposed MRL estimate

- Highest residue 0.030

- Mean + 4 SD 0.052

- CF x 3 Mean 0.029

Unrounded MRL 0.052

Rounded MRL 0.06

High uncert ai nty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 3

0.021 1

0.03 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 132 of 193 Bulb vegetables (group 3

- 07)

The tolerance estimate for subgroup 3

- 07A, based on bulb onion, is 0.04 ppm. The tolerance

estimate for subgroup 3

- 07B is 8 ppm

, based on green onion. A group tolerance is not possible,

as the high and low estimates differ by more than 5X.

Dry bulb onion

Cyantraniliprole

Bulb onion, dry

USA

Foliar 0.40 lb ai /A

Total number of data (n) 10

Percentage of censored data 40%

Number of non

- censored data 6

Lowest residue 0.010

Highest residue 0.027

Median residue 0.014

Mean 0.015

Standard deviation (SD) 0.006

Correction factor for censoring (CF) 0.733

Proposed MRL estimate

- Highest residue 0.027

- Mean + 4 SD 0.038

- CF x 3 Mean 0.033

Unrounded MRL 0.038

Rounded MRL 0.04

Residues (mg/kg) n

< 0.01 4

0.012 1

0.015 1

0.016 1

0.019 1

0.021 1

0.027 1



### Green onion

Cyantraniliprole

Green onion

USACyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 133 of 193 Foliar 0.4 lb ai /A

Total number of data (n) 5

Percentage of censored data 0%

Number of non

- censored data 5

Lowest residue 0.380

Highest residue 4.100

Median residue 1.300

Mean 1.602

Standard deviation (SD) 1.481

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 4.100

- Mean + 4 SD 7.525

- CF x 3 Mean 4.806

Unrounded MRL 7.525

Rounded MRL 8

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.38 1

0.63 1

1.3 1

1.6 1

4.1 1



### Leafy Vegetables (group 4)

The tolerance estimate for the leafy vegetables group 4, based on spinach, is 20 ppm. A group

tolerance is possible because the difference between the low tolerance estimate (head lettuce 5

ppm) and the high tolerance estimate (spinach 20 ppm) is <5X.

Head Lettuce

Cyantraniliprole

Head Lettuce

NAFTA

OD: 3X0.13, 1 d PHI

Total number of data (n) 12

Percentage of censored data 0%

Number of non

- censored data 12

Lowest residue 0.018

Highest residue 2.700Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 1 34 of 193 Median residue 0.695

Mean 0.955

Standard deviation (SD) 0.855

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 2.700

- Mean + 4 SD 4.375

- CF x 3 Mean 2.866

Unrounded MRL 4.375

Rounded MRL 5

Residues (mg/kg) n

0.018 1

0.084 1

0.16 1

0.18 1

0.53 1

0.64 1

0.75 1

1.3 1

1.6 1

1.7 1

1.8 1

2.7 1



### Head Lettuce

1 X 200 g/L SC soil + 2 X 100 g/L OD foliar, 1 day PHI.

One trial. 0.017 ppm HAFT. Insufficient data.

Head Lettuce

Cyantraniliprole

Head Lettuce

USA

SE , 3X0.13, 1 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non

- censored data 6

Lowest residue 0.130

Highest residue 2.100Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 135 of 193 Median residue 0.665

Mean 0.872

Standard deviation (SD) 0.786

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 2.100

- Mean + 4 SD 4.014

- CF x 3 Mean 2.615

Unrounded MRL 4.014

Rounded MRL 4

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.13 1

0.17 1

0.5 1

0.83 1

1.5 1

2.1 1

Head lettuce

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day. One trial.

Insufficient data, 0.9 ppm HAFT.



### Leaf Lettuce

Cyantraniliprole

Leaf Lettuce

USA

OD, 3X0.13, 1 d PHI

Total number of data (n) 12

Percentage of censored data 0%

Number of non

- censored data 12

Lowest residue 1.100

Highest residue 6.800

Median residue 2.650

Mean 3.117

Standard deviation (SD) 1.693

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 6.800

- Mean + 4 SD 9.890Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 136 of 193 - CF x 3 Mean 9.350

Unrounded MRL 9.890

Rounded MRL 10

Residues (mg/kg) n

1.1 1

1.2 1

1.9 1

2.1 1

2.4 2

2.9 1

3.3 1

3.9 1

4 1

5.4 1

6.8 1

Leaf Lettuce

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

One trial. 1.1 ppm HAFT, Insufficient data.

Leaf Lettuce

Cyantraniliprole

Leaf Lettuce

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non

- censored data 6

Lowest residue 2.400

Highest residue 6.800

Median residue 4.250

Mean 4.433

Standard deviation (SD) 1.981

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 6.800

- Mean + 4 SD 12.356

- CF x 3 Mean 13.300

Unrounded MRL 13.300

Rounded MRL 15Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 137 of 193 High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

2.4 1

2.5 1

3.2 1

5.3 1

6.4 1

6.8 1

Leaf lettuce

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day

Insufficient data, 1.7 ppm.



### Celery

Cyantraniliprole

Celery

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non

- censored data 11

Lowest residue 0.280

Highest residue 9.100

Median residue 2.000

Mean 2.783

Standard deviation (SD) 2.684

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 9.100

- Mean + 4 SD 13.517

- CF x 3 Mean 8.348

Unrounded MRL 13.517

Rounded MRL 15

Residues (mg/kg) n

0.28 1

0.73 1

1 1

1.1 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 138 of 193 1.2 1

2 1

2.3 1

2.5 1

4.7 1

5.7 1

9.1 1

Cyantraniliprole

Celery

NAFTA

OD, 2X0.13 , 1 day PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non

- censored data 6

Lowest residue 0.680

Highest residue 5.500

Median residue 1.600

Mean 2.550

Standard deviation (SD) 2.137

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 5.500

- Mean + 4 SD 11.100

- CF x 3 Mean 7.650

Unrounded MRL 11.100

Rounded MRL 15

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.68 1

0.92 1

1.3 1

1.9 1

5 1

5.5 1

Celery

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

One trial. 3.5 ppm HAFT , Insufficient data.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 139 of 193 Celery

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day

One trial

, 3.5 ppm HAFT , Insufficient data.



### Spinach

Cyantraniliprole

Spinach

NAFTA

OD: 3X0.13, 1 d PHI

Total number of data (n) 7

Percentage of censored data 0%

Number of non

- censored data 7

Lowest residue 3.800

Highest residue 13.000

Median residue 4.600

Mean 6.243

Standard deviation (SD) 3.345

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 13.000

- Mean + 4 SD 19.621

- CF x 3 Mean 18.729

Unrounded MRL 19.621

Rounded MRL 20

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

3.8 1

4.1 1

4.2 1

4.6 1

5.8 1

8.2 1

13 1

Spinach

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

One trial, 6.70 ppm HAFT, insufficient data.

Spinach

2 X SC drip irrigation + 1 X OD foliar at 0.13, PHI 1 day

One trial, 6.80 ppm HAFT, insufficient data.

The greatest tolerance estimate, 20 ppm from 3 foliar applications of the OD formulation to spinach, is the

appropriate value. It varies by 5X from the lowest estimate, 4 ppm for head lettuce from three folia r applications of

the SE formulation to head lettuce.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 140 of 193 Brassica Leafy Vegetables (group 5)

The tolerance estimates are 2 ppm for head cabbage, 3 ppm for broccoli/cauliflower, and 30 ppm

for mustard greens. As the estimates vary by a factor of more than 5X from low to high, a group

tolerance is not possible. Based on the head cabbage and brocco li/cauliflower results, a tolerance

of 3 ppm is appropriate for head and stem Brassica vegetables, subgroup 5A. Based on the

mustard green results, a tolerance of 30 ppm is appropriate for Brassica leafy greens, subgroup

5B.

Cabbage

Cyantraniliprole

He ad Cabbage

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non

- censored data 11

Lowest residue 0.290

Highest residue 0.950

Median residue 0.560

Mean 0.556

Standard deviation (SD) 0.221

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.950

- Mean + 4 SD 1.440

- CF x 3 Mean 1.669

Unrounded MRL 1.669

Rounded MRL 2

Residues (mg/kg) n

0.29 1

0.32 2

0.42 1

0.47 1

0.56 1

0.57 1

0.65 1

0.71 1

0.86 1

0.95 1

Head CabbageCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 141 of 193 1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

1 Trial, 0.49 ppm HAFT, insufficient data.



### Mustard Greens

Cyantraniliprole

Mustard Greens

NAFTA

OD, 3X

- 0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non

- censored data 11

Lowest residue 2.400

Highest residue 19.000

Median residue 6.000

Mean 7.391

Standard deviation (SD) 4.773

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 19.000

- Mean + 4 SD 26.482

- CF x 3 Mean 22.173

Unrounded MRL 26.482

Rounded MRL 30

Residues (mg/kg) n

2.4 1

3.4 1

3.9 1

5.5 1

5.8 1

6 1

7.1 1

7.2 1

8 1

13 1

19 1

Mustard Greens

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

1 Trial, 3.1 ppm HAFT, insufficient data.



### Broccoli/CauliflowerCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 142 of 193 Cyantraniliprole

Broccoli/Cauliflower

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 10

Percentage of censored data 20%

Number of non

- censored data 8

Lowest residue 0.010

Highest residue 1.000

Median residue 0.530

Mean 0.468

Standard deviation (SD) 0.333

Correction factor for censoring (CF) 0.867

Proposed MRL estimate

- Highest residue 1.000

- Mean + 4 SD 1.799

- CF x 3 Mean 1.217

Unrounded MRL 1.799

Rounded MRL 2

Residues (mg/kg) n

< 0.01 2

0.22 1

0.28 1

0.51 1

0.55 1

0.59 1

0.69 1

0.82 1

1 1

Cyantraniliprole

Broccoli/Cauliflower

NAFTA

SE, 3X0.13, 1 d PHI

Total number of data (n) 4

Percentage of censored data 0%

Number of non

- censored data 4

Lowest residue 0.470

Highest residue 1.100

Median residue 0.700

Mean 0.743Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 143 of 193 Standard deviation (SD) 0.272

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 1.100

- Mean + 4 SD 1.830

- CF x 3 Mean 2.228

Unrounded MRL 2.228

Rounded MRL 3

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.47 1

0.61 1

0.79 1

1.1 1

Broccoli/Cauliflower

1 X 200 g/L SC soil + 2 X 100 g/L OD, 1 day PHI.

1 Trial, 0.48 ppm HAFT, insufficient data.



### Fruiting vegetables (group 8

- 10)

An appropriate tolerance for the fruiting vegetable group is 1.5 ppm, based on the highest

estimate from the foliar treatments of peppers with the OD formulation in greenhouses. Note

that the greenhouse data are from Europe.

Tomato (field trials)

Cyantraniliprole

Tomato

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 20

Percentage of censored data 0%

Number of non

- censored data 20

Lowest residue 0.041

Highest residue 0.260

Median residue 0.080

Mean 0.099

Standard deviation (SD) 0.053

Correction factor for censoring (CF) 1.000Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 144 of 193 Proposed MRL estimate

- Highest residue 0.260

- Mean + 4 SD 0.311

- CF x 3 Mean 0.297

Unrounded MRL 0.311

Rounded MRL 0.4

Residues (mg/kg) n

0.041 1

0.046 1

0.058 2

0.065 1

0.067 1

0.071 1

0.072 1

0.076 1

0.077 1

0.082 1

0.086 1

0.093 1

0.1 1

0.12 1

0.14 2

0.16 1

0.17 1

0.26 1



### Pepper (field trials)

Cyantraniliprole

Bell Pepper

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 11

Percentage of censored data 0%

Number of non

- censored data 11

Lowest residue 0.032

Highest residue 0.280

Median residue 0.075

Mean 0.119

Standard deviation (SD) 0.086

Correction factor for censoring (CF) 1.000Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 145 of 193 Proposed MRL estimate

- Highest residue 0.280

- Mean + 4 SD 0.465

- CF x 3 Mean 0.358

Unrounded MRL 0.465

Rounded MRL 0.5

Residues (mg/kg) n

0.032 1

0.044 1

0.058 1

0.067 1

0.074 1

0.075 1

0.082 1

0.15 1

0.21 1

0.24 1

0.28 1

Cyantraniliprole

Non

- Bell Pepper

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 9

Percentage of censored data 0%

Number of non

- censored data 9

Lowest residue 0.068

Highest residue 0.420

Median residue 0.180

Mean 0.186

Standard deviation (SD) 0.123

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.420

- Mean + 4 SD 0.679

- CF x 3 Mean 0.557

Unrounded MRL 0.679

Rounded MRL 0.7Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 146 of 193 Residues (mg/kg) n

0.068 1

0.07 1

0.079 1

0.095 1

0.18 1

0.2 1

0.25 1

0.31 1

0.42 1

Bell Peppers

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.092 ppm HAFT, insufficient data

. Non

- Bell Peppers

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.15 ppm HAFT, insufficient data.

Tomatoes

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.048 ppm HAFT, insufficient data.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 147 of 193 Tomato and Pepper in Greenhouse in Europe

Cyantraniliprole Cyantraniliprole Cyantraniliprole Cyantrniliprole Cyantraniliprole

Tomato

(Greenhouse) Tomato

(Greenhouse) Bell Pepper (GH) Pepper

(Greenhouse) Non

- Bell Pepper

(GH)

Europe Europe Europe Europe Europe

OD, 4X0.107,

foliar SC, 4X0.089, drip OD, 4X0.107,

foliar SC, 4X0.089, drip OD, 4X0.107,

foliar

14 8 9 14 5

0% 88% 0% 93% 0%

14 1 9 1 5

0.043 0.010 0.076 0.010 0.086

0.620 0.010 0.170 0.011 1.000

0.165 0.010 0.130 0.010 0.170

0.239 0.010 0.130 0.010 0.343

0.191 0.000 0.030 0.000 0.380

1.000 0.417 1.000 0.381 1.000

0.620 0.010 0.170 0.011 1.000

1.002 0.010 0.250 0.011 1.863

0.717 0.013 0.391 0.012 1.030

1.002 0.013 0.391 0.012 1.863

1 0.015 0.4 0.015 2Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 148 of 193 High uncert ai nty

of MRL estimate

due to high level

of censoring. High uncert ai nty

of MRL estimate

due to high level

of censoring. High uncert ai nty

of MRL estimate

due to small

dataset.

Residues (mg/kg) Residues (mg/kg) Residues (mg/kg) Residues (mg/kg) Residues (mg/kg)

0.043 0.010 * 0.130 0.010 * 0.120

0.180 0.010 * 0.140 0.010 * 1.000

0.140 0.010 * 0.120 0.010 * 0.086

0.140 0.010 * 0.096 0.010 * 0.340

0.072 0.010 * 0.076 0.010 * 0.170

0.180 0.010 * 0.160 0.010 *

0.150 0.010 * 0.130 0.010 *

0.140 0.010 0.150 0.010 *

0.053 0.170 0.010 *

0.220 0.010 *

0.620 0.010 *

0.420 0.010 *

0.590 0.010 *

0.400 0.011Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 149 of 193 Cucurbit vegetables (group 9)

An appropriate tolerance for the cucurbit vegetable group 9 is 0.4 ppm, based on the highest

estimate ( melon ) from the 3 foliar treatments with the OD formulation. The estimates were 0.3

ppm for cucumber, 0.4 ppm for melon, and 0.2 ppm for summer squash, all from 3 foliar

applica tions of the OD formulation at a total rate of about 0.4 lb ai/acre.

Cucumber

Cyantraniliprole

Cucumber

NAFTA

OD, 3X0.13, 1 d PHI

Total number of data (n) 10

Percentage of censored data 0%

Number of non

- censored data 10

Lowest residue 0.018

Highest residue 0.160

Median residue 0.043

Mean 0.057

Standard deviation (SD) 0.047

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.160

- Mean + 4 SD 0.246

- CF x 3 Mean 0.172

Unrounded MRL 0.246

Rounded MRL 0.3

Residues (mg/kg) n

0.018 1

0.021 1

0.023 1

0.029 1

0.041 1

0.045 2

0.07 1

0.12 1

0.16 1

Cyantraniliprole

Melon

NAFTA

OD, 3X0.13, 1 d PHICyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 150 of 193 Total number of data (n) 9

Percentage of censored data 0%

Number of non

- censored data 9

Lowest residue 0.056

Highest residue 0.185

Median residue 0.105

Mean 0.110

Standard deviation (SD) 0.043

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.185

- Mean + 4 SD 0.284

- CF x 3 Mean 0.331

Unrounded MRL 0.331

Rounded MRL 0.4

Residues (mg/kg) n

0.056 1

0.063 1

0.076 1

0.101 1

0.105 1

0.12 1

0.127 1

0.161 1

0.185 1

Cyantraniliprole

Summer Squash

NAFTA

OD, 3X0.13, 1d PHI

Total number of data (n) 9

Percentage of censored data 0%

Number of non

- censored data 9

Lowest residue 0.011

Highest residue 0.110

Median residue 0.059

Mean 0.061

Standard deviation (SD) 0.030

Correction factor for censoring (CF) 1.000Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 151 of 193 Proposed MRL estimate

- Highest residue 0.110

- Mean + 4 SD 0.181

- CF x 3 Mean 0.183

Unrounded MRL 0.183

Rounded MRL 0.2

Residues (mg/kg) n

0.011 1

0.034 1

0.043 1

0.053 1

0.059 1

0.072 1

0.08 1

0.088 1

0.11 1

Summer Squash

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.030 ppm HAFT, insufficient data.

Melon:

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.037 ppm HAFT, insufficient data.

Cucumber:

2 X 200 g/L SC soil + 1 X 100 g/L OD, 1 day PHI.

1 Trial, 0.089 ppm HAFT, insufficient data.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 152 of 193 Citrus fruit (group 10

- 10)

The tolerance estimates are 0.5 ppm for grapefruit, 0.6 ppm for lemon, and 0.7 ppm for orange.

The appropriate tolerance for the citrus group is 0.7 ppm

.



### Orange

Cyantrniliprole

Orange

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 13

Percentage of censored data 0%

Number of non

- censored data 13

Lowest residue 0.098

Highest residue 0.390

Median residue 0.200

Mean 0.209

Standard deviation (SD) 0.096

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.390

- Mean + 4 SD 0.591

- CF x 3 Mean 0.627

Unrounded MRL 0.627

Rounded MRL 0.7

Residues (mg/kg) n

0.098 1

0.1 1

0.12 1

0.13 1

0.15 1

0.17 1

0.2 1

0.21 1

0.22 1

0.28 1

0.3 1

0.35 1

0.39 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 153 of 193 Lemon

Cyantraniliprole

Lemon

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non

- censored data 6

Lowest residue 0.160

Highest residue 0.300

Median residue 0.180

Mean 0.198

Standard deviation (SD) 0.053

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.300

- Mean + 4 SD 0.412

- CF x 3 Mean 0.595

Unrounded MRL 0.595

Rounded MRL 0.6

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.16 2

0.17 1

0.19 1

0.21 1

0.3 1



### Grapefruit

Cyantraniliprole

Grapefruit

USA

SE, 3X0.13, 1 d PHI

Total number of data (n) 7

Percentage of censored data 0%

Number of non

- censored data 7

Lowest residue 0.080

Highest residue 0.310

Median residue 0.140Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 154 of 193 Mean 0.157

Standard deviation (SD) 0.075

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.310

- Mean + 4 SD 0.457

- CF x 3 Mean 0.471

Unrounded MRL 0.471

Rounded MRL 0.5

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.08 1

0.12 2

0.14 2

0.19 1

0.31 1



### Pome fruit (group 11

- 10)

The tolerance for the pome fruit group 11

- 10 is 1.5 ppm, based on the pear data at a 3 day PHI.

The 7 day PHI data were not used for tolerance determination, in accordance with the proposed

label.

Apple

Cyantraniliprole

Apple

NAFTA

SE, 3X0.13, 7 d PHI

Total number of data (n) 16

Percentage of censored data 0%

Number of non

- censored data 16

Lowest residue 0.059

Highest residue 0.300

Median residue 0.125

Mean 0.142

Standard deviation (SD) 0.073

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.300Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 155 of 193 - Mean + 4 SD 0.434

- CF x 3 Mean 0.426

Unrounded MRL 0.434

Rounded MRL 0.5

Residues (mg/kg) n

0.059 1

0.064 1

0.072 1

0.096 1

0.099 1

0.1 1

0.11 1

0.12 1

0.13 2

0.14 1

0.15 1

0.18 1

0.25 1

0.27 1

0.3 1

Cyantraniliprole

Apple

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 16

Percentage of censored data 0%

Number of non

- censored data 16

Lowest residue 0.052

Highest residue 0.31 0

Median residue 0.1 58

Mean 0.168

Standard deviation (SD) 0.078

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.31 0Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 156 of 19 3 - Mean + 4 SD 0.480

- CF x 3 Mean 0. 505

Unrounded MRL 0. 505

Rounded MRL 0.5

Residues (mg/kg) n

0.12 1

0.0 71 1

0. 12 1

0. 26 2

0. 20 1

0. 31 1

0.1 8 1

0. 29 1

0.1 5 1

0. 067 1

0.1 6 2

0. 052 1

0. 17 1

0. 13 1



### Pear

Cyantraniliprole

Pear

NAFTA

SE, 3X0.13, 7 d PHI

Total number of data (n) 10

Percentage of censored data 0%

Number of non

- censored data 10

Lowest residue 0.042

Highest residue 0.560

Median residue 0.160

Mean 0.239

Standard deviation (SD) 0.181

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.560

- Mean + 4 SD 0.963

- CF x 3 Mean 0.716

Unrounded MRL 0.963Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 157 of 193 Rounded MRL 1

Residues (mg/kg) n

0.042 1

0.065 1

0.1 1

0.12 1

0.16 2

0.35 1

0.38 1

0.45 1

0.56 1

Cyantraniliprole

Pear

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 10

Percentage of censored data 0%

Number of non

- censored data 10

Lowest residue 0.0 76

Highest residue 0.58 0

Median residue 0.1 88

Mean 0.2 78

Standard deviation (SD) 0.1 94

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.58 0

- Mean + 4 SD 1.055

- CF x 3 Mean 0. 833

Unrounded MRL 1.055

Rounded MRL 1 .5

Residues (mg/kg) n

0.0 95 1

0. 23 1

0.076 1

0. 54 1

0.15 2

0. 42 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 158 of 193 0. 14 1

0. 12 1

0. 44 1

0.58 1



### Stone fruit (group 12

- 12 )

The recommended tolerances are 0.5 ppm for plum, 1.5 ppm for peach, and 6 ppm for cherry.

The difference between the low and high estimate is >5X. The difference between the estimates

for a small stone fruit (cherry) and a large stone fruit (peach) is >5X. Therefore a stone fruit

group tolerance is not possible, but tolerances may be set for each of the subgroups: cherry

subgroup 12

- 12A at 6 ppm, plum subgroup 12

- 12C at 0.5 ppm, and peach subgroup 12

- 12B at

1.5 ppm.

Peach

Cyantran iliprole

peach

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 13

Percentage of censored data 0%

Number of non

- censored data 13

Lowest residue 0.170

Highest residue 0.960

Median residue 0.340

Mean 0.386

Standard deviation (SD) 0.224

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.960

- Mean + 4 SD 1.281

- CF x 3 Mean 1.158

Unrounded MRL 1.281

Rounded MRL 1.5

Residues (mg/kg) n

0.17 1

0.19 2

0.23 1

0.25 1

0.27 1

0.34 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 159 of 193 0.35 1

0.42 1

0.49 1

0.56 1

0.6 1

0.96 1



### Plums

Cyantraniliprole

Plums

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 9

Percentage of censored data 0%

Number of non

- censored data 9

Lowest residue 0.031

Highest residue 0.280

Median residue 0.066

Mean 0.104

Standard deviation (SD) 0.084

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.280

- Mean + 4 SD 0.441

- CF x 3 Mean 0.313

Unrounded MRL 0.441

Rounded MRL 0.5

Residues (mg/kg) n

0.031 1

0.044 1

0.052 1

0.064 1

0.066 1

0.071 1

0.13 1

0.2 1

0.28 1



### Cherry

Cyantraniliprole

Cherry (sweet/tart)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 160 of 193 NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 7

Percentage of censored data 0%

Number of non

- censored data 7

Lowest residue 0.330

Highest residue 3.800

Median residue 0.930

Mean 1.179

Standard deviation (SD) 1.190

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 3.800

- Mean + 4 SD 5.938

- CF x 3 Mean 3.536

Unrounded MRL 5.938

Rounded MRL 6

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.33 1

0.36 1

0.89 1

0.93 1

0.96 1

0.98 1

3.8 1



### Blueberry (subgroup 13

- 07B)

The tolerance estimate for bushberry subgroup 13

- 07B is 4 ppm, based on

blueberry field trial data.

C yantraniliprole

blueberry

NAFTA

SE, 3X0.13, 3 d PHI

Total number of data (n) 8

Percentage of censored data 0%

Number of non

- censored data 8

Lowest residue 0.420

Highest residue 2.000Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 161 of 193 Median residue 0.675

Mean 0.888

Standard deviation (SD) 0.563

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 2.000

- Mean + 4 SD 3.139

- CF x 3 Mean 2.663

Unrounded MRL 3.139

Rounded MRL 4

Residues (mg/kg) n

0.42 1

0.51 1

0.52 1

0.6 1

0.75 1

0.8 1

1.5 1

2 1



### Tree Nuts (group 14)

The tolerance estimate for tree nuts is 0.04 ppm, based on the almond field trial data. The

tolerance estimate for almond hulls is 8 ppm.

Almond

Cyantrniliprole

Almond nutmeat

USA

OS, 3X0.13, 5 d PHI

Total number of data (n) 6

Percentage of censored data 67%

Number of non

- censored data 2

Lowest residue 0.010

Highest residue 0.023

Median residue 0.010

Mean 0.013

Standard deviation (SD) 0.005

Correction factor for censoring (CF) 0.556

Proposed MRL estimate

- Highest residue 0.023Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 162 of 193 - Mean + 4 SD 0.033

- CF x 3 Mean 0.021

Unrounded MRL 0.033

Rounded MRL 0.04

High uncert ai nty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 4

0.012 1

0.023 1

Almond Nutmeat

SE, 3X0.13, 5 d PHI

2 trials: 0.007, 0.018 ppm. Insufficient data for estimate. Residues do not exceed the tolerance estimate for the OD

formulation.

Cyantraniliprole

Almond hulls

USA

OD, 3X0.13, 5 d PHI

Total number of data (n) 6

Percentage of censored data 0%

Number of non

- censored data 6

Lowest residue 0.880

Highest residue 4.600

Median residue 1.600

Mean 2.082

Standard deviation (SD) 1.447

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 4.600

- Mean + 4 SD 7.871

- CF x 3 Mean 6.245

Unrounded MRL 7.871

Rounded MRL 8

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.88 1

0.91 1

1.3 1

1.9 1

2.9 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 163 of 193 4.6 1

Almond Hulls

Two trials, SE formulation, 3X0.13 lb ai /acre, 5 day PHI

2.5 ppm and 3.6 ppm are below the tolerance estimate (8 ppm) for us of the OD formulation.

Cyantraniliprole

Pecan nutmeat

USA

OD, 3X0.13, 5 d PHI

Total number of data (n) 6

Percentage of censored data 100%

Number of non

- censored data 0

Lowest residue 0.010

Highest residue 0.010

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.333

Proposed MRL estimate

- Highest residue 0.010

- Mean + 4 SD 0.010

- CF x 3 Mean 0.010

Unrounded MRL 0.010

Rounded MRL 0.01

High uncert ai nty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 6



### Pecan

Subsurface side

- dress, SC, 1X0.41 lb ai /acre, PHI 57 days

One trial, cyantraniliprole and all metabolites each ND (<0.01 ppm).



### Oilseed (group 20)

The tolerance estimate for the oilseed group 20 is 1.5 ppm, based on cotton field trial data . The

OECD procedure calculated tolerances are 0.5 ppm for sunflower seed, 0.8 ppm for canola seed,

and 1.5 ppm for cottonseed. The high and low values differ by a factor of < 5X. The toleran ce

estimate for cotton gin byproducts is 10 ppm.

Cotton

Cyantraniliprole

Cottonseed

NAFTACyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 164 of 193 OD, 3X0.13, 7 d PHI

Total number of data (n) 13

Percentage of censored data 0%

Number of non

- censored data 13

Lowest residue 0.012

Highest residue 0.990

Median residue 0.160

Mean 0.212

Standard deviation (SD) 0.249

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.990

- Mean + 4 SD 1.209

- CF x 3 Mean 0.635

Unrounded MRL 1.209

Rounded MRL 1.5

Residues (mg/kg) n

0.012 1

0.025 1

0.035 1

0.12 2

0.14 1

0.16 1

0.18 1

0.2 1

0.22 1

0.26 1

0.29 1

0.99 1

1 X 0.17 lb ai /A in

- furrow with SC + 2 X 0.13 lb ai /A foliar

One study, 0.15 ppm

Insufficient data for estimation of tolerance.

Cyantraniliprole

Cotton gin byprod

NAFTA

OD, 3X0.13, 7 d PHI

Total number of data (n) 4Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 165 of 193 Percentage of censored data 0%

Number of non

- censored data 4

Lowest residue 2.600

Highest residue 5.000

Median residue 3.100

Mean 3.450

Standard deviation (SD) 1.109

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 5.000

- Mean + 4 SD 7.886

- CF x 3 Mean 10.350

Unrounded MRL 10.350

Rounded MRL 10

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

2.6 1

2.7 1

3.5 1

5 1



### Canola

Cyantraniliprole

Canola

NAFTA

OD, 3X0.13, 7 d PHI

Total number of data (n) 16

Percentage of censored data 0%

Number of non

- censored data 16

Lowest residue 0.017

Highest residue 0.610

Median residue 0.102

Mean 0.157

Standard deviation (SD) 0.156

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.610

- Mean + 4 SD 0.779

- CF x 3 Mean 0.470

Unrounded MRL 0.779Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 166 of 193 Rounded MRL 0.8

cyantraniliprole

Canola

NAFTA

seed + 3 foliar

Total number of data (n) 4

Percentage of censored data 0%

Number of non

- censored data 4

Lowest residue 0.015

Highest residue 0.210

Median residue 0.039

Mean 0.076

Standard deviation (SD) 0.090

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.210

- Mean + 4 SD 0.438

- CF x 3 Mean 0.227

Unrounded MRL 0.438

Rounded MRL 0.5

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.015 1

0.031 1

0.047 1

0.21 1



### Sunflower

Cyantraniliprole

Sunflower seed

NAFTA

OD, 3X0.13, 7 d PHI

Total number of data (n) 8

Percentage of censored data 0%

Number of non

- censored data 8Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 167 of 193 Lowest residue 0.028

Highest residue 0.320

Median residue 0.066

Mean 0.100

Standard deviation (SD) 0.095

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.320

- Mean + 4 SD 0.480

- CF x 3 Mean 0.301

Unrounded MRL 0.480

Rounded MRL 0.5

Residues (mg/kg) n

0.028 1

0.039 1

0.059 1

0.064 1

0.067 1

0.085 1

0.14 1

0.32 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 168 of 193 Tolerances for Raw Agricultural Commodities from Rotational Crop Field Trials

Root and Tuber Vegetables, Subgroup 1A

Rotational field trials with a 30 day PBI were conducted for turnip (n = 2), sugar beet (n = 1 4 ),

carrot (n = 10 ), garden beet (n = 1 ), and radish (n =6). Residues were <0.01 for sugar beet root,

garden beet root, and turnip root (n=2). Quantifiable residues were encountered with carrot and

radish root. The tolerance estimate is 0.02 ppm, based on radish root. The value of 0.03 ppm

petitioned by the registrant is too high, based on the data submitted.

Cyantraniliprole

Carrot (rotational)

NAFTA

1X seasonal

Total number of data (n) 10

Percentage of censored data 90%

Number of non

- censored data 1

Lowest residue 0.010

Highest residue 0.011

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.400

Proposed MRL estimate

- Highest residue 0.011

- Mean + 4 SD 0.011

- CF x 3 Mean 0.012

Unrounded MRL 0.012

Rounded MRL 0.015

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 9

0.011 1

Cyantraniliprole

Radish root (rotational)

NAFTA

1X seasonal

Total number of data (n) 6Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 169 of 193 Percentage of censored data 83%

Number of non

- censored data 1

Lowest residue 0.010

Highest residue 0.014

Median residue 0.010

Mean 0.011

Standard deviation (SD) 0.002

Correction factor for censoring (CF) 0.444

Proposed MRL estimate

- Highest residue 0.014

- Mean + 4 SD 0.017

- CF x 3 Mean 0.014

Unrounded MRL 0.017

Rounded MRL 0.02

High uncert ai nty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 5

0.014 1



### Leaves of root and tuber vegetables (human food or animal feed) (crop group 2)

Trials were conducted for turnip tops (n = 6), sugar beet tops (n = 1 3 ), garden beet top (n = 1),

and radish tops (n = 3). No quantifiable residues were found with garden beet tops and sugar

beet tops. The OECD calculator provides an estimate of the tolerance of 0.04 ppm for leaves of

root and tuber vegetables (crop group 2), based on radish tops. This ag r ees with the estimate of

0.04 ppm from the registrant.

Cyantraniliprole

Turnip tops (rotational)

NAFTA

1X seasonal

Total number of data (n) 6

Percentage of censored data 83%

Number of non

- censored data 1

Lowest residue 0.010

Highest residue 0.020

Median residue 0.010

Mean 0.012

Standard deviation (SD) 0.004

Correction factor for censoring (CF) 0.444

Proposed MRL estimateCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 170 of 193 - Highest residue 0.020

- Mean + 4 SD 0.028

- CF x 3 Mean 0.016

Unrounded MRL 0.028

Rounded MRL 0.03

High uncert ai nty of MRL estimate.

[Small dataset and high level of censoring]

Residues (mg/kg) n

< 0.01 5

0.02 1

Cyantraniliprole

Radish top

NAFTA

1X Seasonal

Total number of data (n) 3

Percentage of censored data 33%

Number of non

- censored data 2

Lowest residue 0.010

Highest residue 0.021

Median residue 0.011

Mean 0.014

Standard deviation (SD) 0.006

Correction factor for censoring (CF) 0.778

Proposed MRL estimate

- Highest residue 0.021

- Mean + 4 SD 0.038

- CF x 3 Mean 0.033

Unrounded MRL 0.038

Rounded MRL 0.04

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

< 0.01 1

0.011 1

0.021 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 171 of 193 Foliage of legume vegetables , including soybean (crop group 7)

Eight trials were conducted for peas and beans. Seed (succulent and dry) cyantraniliprole

concentrations were <0.01 ppm and detectable in only one instance. Finite residues were found

on 3 forage samples and on 5 hay samples.

Sixteen rotational crop trials were conducted with soybean. No detectable residue was found on

pod or seed (n = 13). The OECD calculator is used to estimate tolerances for soybean fora ge and

soybean hay.

The OECD estimates based on pea and bean data (0.3 ppm) are lower than those from soybean.

The tolerance values are 0.2 ppm for soybean forage and 0.7 ppm for soybean hay. The

registrant proposes 0.15 ppm for forage and 0.6 ppm fo r hay. Only one value may be used for

this crop group, and the 0.6 ppm value is too low. The OECD estimate of the tolerance is 0.7

ppm, based on soybean hay.

Cyantraniliprole

Bean/Pea Forage (rotational)

NAFTA

1X seasonal

Total number of data (n) 8

Percentage of censored data 63%

Number of non

- censored data 3

Lowest residue 0.010

Highest residue 0.031

Median residue 0.010

Mean 0.016

Standard deviation (SD) 0.009

Correction factor for censoring (CF) 0.583

Proposed MRL estimate

- Highest residue 0.031

- Mean + 4 SD 0.050

- CF x 3 Mean 0.028

Unrounded MRL 0.050

Rounded MRL 0.05

High uncert ai nty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 5

0.019 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 172 of 193 0.026 1

0.031 1

Cyantraniliprole

Bean/Pea Hay (rotational)

NAFTA

1X seasonal

Total number of data (n) 8

Percentage of censored data 38%

Number of non

- censored data 5

Lowest residue 0.010

Highest residue 0.130

Median residue 0.022

Mean 0.054

Standard deviation (SD) 0.055

Correction factor for censoring (CF) 0.750

Proposed MRL estimate

- Highest residue 0.130

- Mean + 4 SD 0.274

- CF x 3 Mean 0.122

Unrounded MRL 0.274

Rounded MRL 0.3

Residues (mg/kg) n

< 0.01 3

0.017 1

0.027 1

0.11 1

0.12 1

0.13 1

Cyantaniliprole

Soybean forage

NAFTA

1X seasonal

Total number of data (n) 16

Percentage of censored data 69%

Number of non

- censored data 5

Lowest residue 0.010

Highest residue 0.140

Median residue 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 173 of 193 Mean 0.026

Standard deviation (SD) 0.037

Correction factor for censoring (CF) 0.542

Proposed MRL estimate

- Highest residue 0.140

- Mean + 4 SD 0.173

- CF x 3 Mean 0.043

Unrounded MRL 0.173

Rounded MRL 0.2

High uncert ai nty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 11

0.016 1

0.024 1

0.038 1

0.091 1

0.14 1

Cyantraniliprole

Soybean hay

NAFTA

1X seasonal

Total number of data (n) 16

Percentage of censored data 19%

Number of non

- censored data 13

Lowest residue 0.010

Highest residue 0.580

Median residue 0.017

Mean 0.066

Standard deviation (SD) 0.143

Correction factor for censoring (CF) 0.875

Proposed MRL estimate

- Highest residue 0.580

- Mean + 4 SD 0.639

- CF x 3 Mean 0.174

Unrounded MRL 0.639

Rounded MRL 0.7Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 174 of 193 Residues (mg/kg) n

< 0.01 3

0.011 2

0.013 2

0.015 1

0.018 1

0.024 1

0.029 1

0.033 1

0.048 1

0.055 1

0.18 1

0.58 1



### Forage, fodder and straw of cereal gr ai ns (crop group 16)

There are 3 trials for oats, 2 for field corn, 2 for sweet corn, 3 for sorghum, 3 for wheat, and 1 for

rice. The various forages, straws, and hays were estimated individually by crop type . The

highest estimate of the tolerance is 0.5 ppm d erived from 3 trials on wheat hay. The registrant

proposed 0.06 ppm for cereal gr ai n forage and 0.15 ppm for cereal gr ai n hay and straw. Only

one tolerance value is appropriate for a crop group, and the 0.15 ppm proposal is too low. The

estimate is 0.5 ppm.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 175 of 193Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 176 of 193 Compound Cyantraniliprole Cyantraniliprole Cyantraniliprole

Crop Oats hay

(rotational) wheat hay

(rotational) combined hay

(rotational)

Region / Country

GAP 1X seasonal 1X seasonal 1X seasonal

Total number of data (n) 3 3 6

Percentage of censored data 0 % 0% 17%

Number of non

- censored data 3 3 5

Lowest residue 0.01 9 0.022 0.010

Highest residue 0.091 0.200 0.200

Median residue 0.059 0.140 0.058

Mean 0.05 6 0.121 0.081

Standard deviation (SD) 0.0 36 0.091 0.077

Correction factor for censoring

(CF) 1.000 1.000 0.889

Proposed MRL estimate

- Highest residue 0.091 0.200 0.200

- Mean + 4 SD 0.2 01 0.483 0.388

- CF x 3 Mean 0.1 69 0.362 0.217

Unrounded MRL 0.2 01 0.483 0.388

Rounded MRL 0. 2 0.5 0.4

High uncert ai nty of

MRL estimate due to

small dataset. High uncert ai nty of

MRL estimate due to

small dataset. High uncert ai nty of MRL

estimate due to small

dataset.

Residues (mg/kg) Residues (mg/kg) Residues (mg/kg)

0.059 0.022 0.025

0.091 0.200 0.091

0.01 9 0.140 0.010 *

0.022

0.200

0.140

Cyantraniliprole

Hay All (Wheat + Straw)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 177 of 193 NAFTA Ro tational

1X seasonal

Total number of data (n) 6

Percentage of censored data 0%

Number of non

- censored data 6

Lowest residue 0.019

Highest residue 0.200

Median residue 0.075

Mean 0.089

Standard deviation (SD) 0.071

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.200

- Mean + 4 SD 0.373

- CF x 3 Mean 0.266

Unrounded MRL 0.373

Rounded MRL 0.4

High uncertainty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.019 1

0.022 1

0.059 1

0.091 1

0.14 1

0.2 1Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 178 of 193Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 179 of 193 G rass forage, fodder, and hay (crop group 17)

Rotational crop trials were conducted for Bermuda grass (n = 2), bromegrass (n = 1), and

bluegrass (n = 1). The crop results were combined (n = 4) for purposes of tolerance estimation.

The tolerance estimate is 0.5 ppm, based on hay. The registrant proposed 0.06 ppm for forage

and 0.15 ppm for hay. However, only one tolerance value may be established for a group, and

the 0.15 ppm value is too low.

Cyantraniliprole

Grass forage

NAFTA

1X seasonal

Total number of data (n) 4

Percentage of censored data 25%

Number of non

- censored data 3

Lowest residue 0.010

Highest residue 0.089

Median residue 0.011

Mean 0.030

Standard deviation (SD) 0.039

Correction factor for censoring (CF) 0.833

Proposed MRL estimate

- Highest residue 0.089

- Mean + 4 SD 0.187

- CF x 3 Mean 0.076

Unrounded MRL 0.187

Rounded MRL 0.2

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

< 0.01 1

0.011 2

0.089 1

Cyantraniliprole

Grass hay (rotat)

NAFTA

1X seasonal

Total number of data (n) 4

Percentage of censored data 0%

Number of non

- censored data 4

Lowest residue 0.011Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 180 of 193 Highest residue 0.220

Median residue 0.024

Mean 0.070

Standard deviation (SD) 0.100

Correction factor for censoring (CF) 1.000

Proposed MRL estimate

- Highest residue 0.220

- Mean + 4 SD 0.471

- CF x 3 Mean 0.209

Unrounded MRL 0.471

Rounded MRL 0.5

High uncert ai nty of MRL estimate.

[Small dataset]

Residues (mg/kg) n

0.011 1

0.023 1

0.025 1

0.22 1



### Nongrass animal feeds (forage, fodder, straw, and hay) (crop group 18)

Rotational field trials were conducted for alafafa (n =12) and clover (n = 11). The tolerance

estimate is 0.2 ppm based on alfalfa hay. The registrant proposed 0.06 ppm for forage and 0.15

ppm for hay. However, only one tolerance value may be set for a group, and the 0.15 ppm value

is too low.

Cyantraniliprole

alfalfa forage

NAFTA

1X seasonal

Total number of data (n) 12

Percentage of censored data 83%

Number of non

- censored data 2

Lowest residue 0.010

Highest residue 0.053

Median residue 0.010

Mean 0.016

Standard deviation (SD) 0.014

Correction factor for censoring (CF) 0.444

Proposed MRL estimate

- Highest residue 0.053

- Mean + 4 SD 0.073Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 181 of 193 - CF x 3 Mean 0.021

Unrounded MRL 0.073

Rounded MRL 0.08

High uncert ai nty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 10

0.038 1

0.053 1

Cyantraniliprole

Clover forage

NAFTA

1X seasonal

Total number of data (n) 11

Percentage of censored data 91%

Number of non

- censored data 1

Lowest residue 0.010

Highest residue 0.023

Median residue 0.010

Mean 0.011

Standard deviation (SD) 0.004

Correction factor for censoring (CF) 0.394

Proposed MRL estimate

- Highest residue 0.023

- Mean + 4 SD 0.027

- CF x 3 Mean 0.013

Unrounded MRL 0.027

Rounded MRL 0.03

High uncert ai nty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 10

0.023 1

Cyantraniliprole

Clover HayCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 182 of 193 NAFTA

1X seasonal

Total number of data (n) 11

Percentage of censored data 73%

Number of non

- censored data 3

Lowest residue 0.010

Highest residue 0.045

Median residue 0.010

Mean 0.017

Standard deviation (SD) 0.013

Correction factor for censoring (CF) 0.515

Proposed MRL estimate

- Highest residue 0.045

- Mean + 4 SD 0.067

- CF x 3 Mean 0.026

Unrounded MRL 0.067

Rounded MRL 0.07

High uncert ai nty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 8

0.029 1

0.033 1

0.045 1

Cyantraniliprole

Alfalfa Hay

NAFTA Rotational

1X Seasonal

Total number of data (n) 12

Percentage of censored data 67%

Number of non

- censored data 4

Lowest residue 0.010

Highest residue 0.140

Median residue 0.010

Mean 0.029

Standard deviation (SD) 0.043

Correction factor for censoring (CF) 0.556

Proposed MRL estimateCyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 183 of 193 - Highest residue 0.140

- Mean + 4 SD 0.201

- CF x 3 Mean 0.048

Unrounded MRL 0.201

Rounded MRL 0.2

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 8

0.01 1

0.021 1

0.097 1

0.14 1



### Peanut hay

Thirteen rotational crop trials were conducted with peanut. The cyantraniliprole residue was

non

- detectable in all nutmeat samples. Cyantraniliprole was detected in 6 hay samples, but

nonquantifiable (<0.01 ppm) in all 11 samples. A n inadvertent tolerance estimate is not needed

for peanut nutmeat as there is no reasonable expectation of residues of cyantraniliprole. Using

the OECD calculator, the tolerance estimate for peanut hay is 0.01 ppm. The petitioner proposed

0.03 ppm, but this value is too high.

Cyantraniliprole

Peanut hay

NAFTA

1X seasonal

Total number of data (n) 13

Percentage of censored data 100%

Number of non

- censored data 0

Lowest residue 0.010

Highest residue 0.010

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.333

Proposed MRL estimate

- Highest residue 0.010

- Mean + 4 SD 0.010

- CF x 3 Mean 0.010

Unrounded MRL 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 184 of 193 Rounded MRL 0.01

High uncert ai nty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 13



### Strawberry

Nine rotational crop trials were conducted with strawberry with a 30 day PBI. Values ranged

from non

- detectable to a maximum of 0.004 ppm (<0.01 LOQ). The OECD calculator estimates

a tolerance of 0.01 ppm for crop subgroup 13

- 07G (strawberry). This represents a situation in

which no residues are anticipated. The petitioner did not request a n inadvertent tolerance, and

none is needed.

The intervals from planting to harvest of the strawberries were 3

– 5 months

. Some commercial

production does not begin major harvest until about one year after planting. However

, i n

California (80% of US production) , perennial strawberries are generally managed as an annual

crop and are removed one year after planting, although 6

– 7% of the acreage is keep for a

second year of harvesting. Most fields are annually replanted from nursery stock to maximize

crop vigor, avoid pest build

- up in the soil, and maintain fruit quality throughout the harvest

period. ( The Californ ia Strawberry Commission (CSC); The California Minor Crops Council

(CMCC))

Cyantraniliprole

Strawberry

NAFTA Rotational

1X Seasonal

Total number of data (n) 9

Percentage of censored data 100%

Number of non

- censored data 0

Lowest residue 0.010

Highest residue 0.010

Median residue 0.010

Mean 0.010

Standard deviation (SD) 0.000

Correction factor for censoring (CF) 0.333

Proposed MRL estimate

- Highest residue 0.010

- Mean + 4 SD 0.010

- CF x 3 Mean 0.010

Unrounded MRL 0.010Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 185 of 193 Rounded MRL 0.01

High uncertainty of MRL estimate.

[High level of censoring]

Residues (mg/kg) n

< 0.01 9



### Tolerances for Processed Commodities

Cyantraniliprole concentrated relative to the RAC in the following commodities: dry tomato

pomace (1.8X), orange oil (6.2X), apple pomace dry (2.6X), and dried plum prune (1.5X).

Tomato pomace is not a livestock feed item in the US, and dry apple pomace i s not a feed item in

the US. Wet apple pomace is fed, but no concentration was observed in the wet matrix.

Cyantraniliprole concentrated slightly in apple puree (1.1X), but tolerances are not established

for this commodity.

The OECD statistical calculati on procedure is not used for processed commodities. Rather, the

HAFT from the relevant field trials is multiplied by the median processing factor to arrive at an

estimate.

Citrus Oil

The median processing factor for citrus oil is 6.2X. Application of thi s factor to the HAFT

(orange, grapefruit, lemon), 0.39 ppm for orange, yields 2.4 ppm, that is 6.2 X 0.39. The

tolerance estimate for citrus oil is 2.4 ppm.

Dried Plum (Prune)

The median processing factor for dried plum is 1.5. Application of this fac tor to the HAFT of

plum, 0.28 ppm, yields 0.42 ppm, that is, 1.5 X 0.28.

. As 0. 42 ppm is less than the

recommended tolerance for the plum subgroup 12

- 12C at 0 .5 ppm, a dried plum tolerance is not

needed.Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 186 of 193 APPENDIX I II: Number and Distribution of Fie ld Trials for Various Crops Tested

NAFTA

Growing

Regions TUBEROUS AND CORM VEGETABLES

– CROP SUBGROUP 1C

POTATO TUBERS

No. of Trials Submitted Trial Requirements

CAN USA

1 7 7 2

2 1 1

3 1

4

5 4 5 2

6

7 2

8

9 1 1

10 1 1

11 5 4

12

13

14 1 2

15

- 21

Total 21 16 12

BULB VEGETABLES

– CROP GROUP 3

NAFTA

Growing

Region DRY BULB ONIONS GREEN ONIONS

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA

1 1 1

2 1

3

4

5 5 5 2 3

6 1 1 1 1

7

8 1 1

9

10 2 2 1 2

11 1

12

13

- 21

Total 10 5 6 5 3 3

CROP GROUP 4

– LEAFY VEGETABLES

NAFTA

Growing

Region HEAD LETTUCE LEAF LETTUCE CELERY SPINACH

No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA CAN USA

1 1 1* 1 1* 1 1

2 1* 1* 1 1

3 1 1 1 1 1 1

4 2 3

5 4 5 4 5 5 5 1 1 1

6

7

8

9 1 1

10 5 4 5 4 5 4 3 2

11

12 1 1 1

13

- 21Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 187 of 193 Total 12 5 6 12 5 6 11 5 6 10 3 6

NAFTA

Growing

Region HEAD AND STEM BRASSICA VEGETABLES LEAFY BRASSICA

CSG 5A CSG 5A CSG 5A CSG 5B

BROCCOLI CAULIFLOWER CABBAGE MUSTARD GREENS

No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA CAN USA

1 1 1 1 1 2

2 1 1 2 1

3 1 1 1

4 1 1

5 4 4 4 1 4 5 1 1 2 1

6 1 1 1 1 1

7

8

9

10 4 4 4 3 2 1 2 1

11

12 1 1 1 1 1 1 1

13

- 21

Total 9 5 6 4 5 6 11 5 6 11 3 5

FRUITING VEGETABLES

– CROP GROUP 8

- 09

NAFTA

Growing

Region TOMATOES BELL PEPPERS NON

- BELL PEPPERS

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA CAN USA

1 1 1

2 1 1 1 1

3 2 2 1 1

4

5 8 12 1 5 5 1 5 5

6 1 1 1 1

7

8

9

10 8 7 3 2 3 2

11

12

13

- 21

Total 20 12 12 11 5 6 9 5 3

CUCURBIT VEGETABLES

– CROP GROUP 9

NAFTA

Growing

Region CUCUMBER CANTALOUPES SUMMER SQUASH

No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA

1 1 1

2 2 2 1 1 1 1

3 2 1 1 1

4

5 4 5 2 3 5 1 3 4 1

6 1 1 1 1

7

8

9

10 4 3 2 1

11

12 1 1

13

- 21

Total 10 5 6 9 5 6 9 5 5Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 188 of 193 CITRUS FRUITS

– CROP GROUP 10

NAFTA

Growing

Region ORANGES GRAPEFRUITS LEMONS

No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA

1

2

3 8 8 3 3 1

4

5

6 1 1 1 1

7

8

9

10 4 3 3 2 6 4

11

12

13

- 21

Total 13 12 7 6 6 5

POME FRUITS

– CROP GROUP 11

NAFTA

Growing

Region APPLES PEARS

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA

1 3 3 3 1 1

2 1 1

3

4

5 5 7 2 4 4

6

- 8

9 1 1

10 1 1 2 2

11 4 2 4 4 1 3

12

- 21

Total 15 12 12 11 5 6

STONE FRUITS

- CROP GROUP 12

- 09

NAFTA

Growing

Region PEACHES PLUMS CHERRIES

No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requiremen

ts No. of Trials

Submitted Trial Requirements

CAN USA CA

N US

A CAN USA

SC 1 TC 2 SC 1 TC 2 SC 1 TC 2

1 1 1 1 1

2 4 3

3

4 1

5 2 4 1 2 2 1 3 1 4 2 4

6 1 1

7

8

9 1 1 1

10 3 3 5 4 1 2

11 1 1 1 1 1 3 2

12 1 1

13

14 1

15

- 21

Total 13 5 9 9 3 6 2 5 5 5 6 6Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 189 of 193 BLUEBERRIES

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1 4 4 1

2 2* 3

3

4

5 3 3 3

6

7

8

9

10

11

12 1 1 1

13

- 21

Total 10 8 8

TREE NUTS

– CROP GROUP 14

NAFTA

Growing

Region ALMONDS PECANS

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA

1

2 3 2

3

4 1 1

5

6 1 1

7

8 1 1

9

10 6 5

11

12

13

- 21

Total 6

-- 5 6

-- 5

COTTON

NAFTA

Growing Regions No. of Trials

Submitted Trial Requirements

CAN USA

1

2 1 1

3

4 3 3

5

6 1 1

7

8 5 4

9

10 3 3

11

12

13

- 21

Total 13

-- 12Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 190 of 193 OILSEED

– CROP GROUP 20

NAFTA

Growing

Region CANOLA SUNFLOWERS

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA

1

2 1 1

3

4

5 2 1 2 2 3 2

6

7 1 2 1 4 3

8 1 1

9

10

11 2 2

12

13

14 11 9 2 2

15

- 21

Total 17 12 6 9 5 6Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 191 of 193 Rotational Crops

The number and distribution of crop field trial guidance is taken from OCSPP 860.1500 and is

based on requirements for primary crops. Some latitude is appropriate for those commodities

which are livestock feed items only.

NAFTA

Growing

Region ROOT VEGETABLES

– CROP SUBGROUP 1A 1

CARROT RADISH SUGAR BEET

No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA

1 1 1 1

2 2

3 1 1 2

4

5 3 1 1 1 6 5

6 1 1

7 4 1

8 1 1

9

10 3 3 1 1 1 1

11 1 1 2 1

12

13

- 21

Total 10 6 6 5 14 9

1 Trials were also provided for turnip root (n =2) and garden beet (n = 1).

LEAVES OF ROOT AND TUBER VEGETABLES

- CROP GROUP 2 1

NAFTA

Growing

Region TURNIP SUGAR BEET

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA

1

2 2 2

3

4 1

5 3 1 4 5

6 1 1

7 4 1

8 1 1

9

10 1 1

11 2 1

12

13

14 1

15

- 21

Total 6 5 13 9

1 Trials were also provided for radish top (n =3) and garden beet top (n = 1)Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 192 of 193 FOLIAGE OF LEGUME VEGETABLES

– CROP GROUP 7

NAFTA

Growing

Region BEANS PEAS SOYBEAN

No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements No. of Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA

1

2 1 1 2

3

4 2

5 1 2 9 11

6

7 3

8

9

10 1

11 2 2 2

12

13

- 21

Total 4 3 4 3 16 15

NAFTA

Growing

Region FORAGE,FODDER AND S T RAW OF CEREAL GRAINS

– CROP GROUP 16 1

Corn (field) Oats Sorghum Wheat

No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements No. of

Trials

Submitted Trial

Requirements

CAN USA CAN USA CAN USA CAN USA

1 1 1

2 2 1 1 1 1

3

4 1 1

5 2 12 1 6 2 3 1 3

6 1 1 2 1

7 2 1 4

8 1 2 4

9

10 1 1

11 1 1 1

12

13

- 21

Total 2 4 15 3

- 3 3

- 3 3 15

1 One trial was also provided for rice. Total provided = 14

2 Total = 42.

3 Forages/fodders/straws other than corn and wheat should total 12.

GRASS FORAGE, FODDER, AND HAY

– CROP GROUP 17

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1

2

3

4

5 3

6

7

8

9

10 1

11

12

13

- 21Cyantraniliprole Summary of Analytical Chemistry and Residue Data DP#: 407961



### Page 193 of 193 Total 4 9

NONGRASS ANIMAL FEEDS

– CROP GROUP 1 8

NAFTA

Growing

Region ALFALFA CLOVER

No. of Trials

Submitted Trial Requirements No. of Trials

Submitted Trial Requirements

CAN USA CAN USA

1 1 1 2 1

2 1

3

4 1

5 3 4 3 2

6 1 1

7 3 1 2 1

8 1

9 1

10 2 1 1 1

11 1 1 1

12

13

14 2 1

15

- 21

Total 12 9 11 9

PEANUT HAY

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1

2 9 5

3 1 1

4

5

6 3 2

7

8 1

9

10

11

12

13

- 21

Total 13 9 1

1 For a no residue situation. Otherwise, 12 trials.

LOW GROWING BERRY SUBGROUP 13

- 07G (STRAWBERRY)

NAFTA Growing Regions No. of Trials Submitted Trial Requirements

CAN USA

1 2 1

2

3 1 1

4

5 1 1

6

7

8

9

10 4 2

11

12 1 1

13

- 21

Total 9 6 1

1 Number required for strawberry as part of crop group or more a no residue situation. Otherwise, 8.