UNITED STATES ENVIRONMENTAL PROTECTIONAGENCY WASHINGTON D.C., 20460
OFFI( F 01· (HE\I'C\L SAFFn \'\D POI.I,I TlO'\ PRF\T'\TJO'\ 

### PC Code: 080803 

### DP Barcode: 394938 Date: October 13, 2011
MEMORANDUM SUBJECT:

TO:

FROM:

REVIEWED BY:


### APPROVED BY: Environmental Fate and Effects Division Review ofOpen Literature Microcosm and Mesocosm Studies for Analysis ofAtrazine Risks to Aquatic Plant Communities Melanie Biscoe, Chemical Review Manager Risk Management and Implementation Branch IV Pesticide Re-evaluation Division (7508P) FrankT. Farruggia, PhD., Biologist

# G6>~**/~,/;**

# (

Environmental Risk Branch III Environmental Fate and Effects Division**(7507P)_~\** 

# i\ .,-- (
Rosanna Louie-Juzwiak, RAPL 

# !:J P .c..

# if? /,,- Environmental Risk Branch III \1 ) ,i " Environmental Fate and Effects Div . n (7507P) ) ,- Dana Spatz, Branch ('h,i"'r-

.... Environmental Risk

### B~~ra·:n~c~,**~~~~~~~~:$~::--** Environmental Fate and Effects Division (7507P)
This memo transmits copies ofthe DataEvaluation Records (DERs) developed for the open literature studies referred to in our documents titled "Bibliography ofMicrocosm and Mesocosm Studies and CriteriaUsedto Screen Studies for Analysis ofAtrazine Risks to Aquatic Plant Communities," datedAugust 25,2011. Study classifications from that document are also provided here for your convenience. Please find the attached data evaluation records (DER) for the following studies evaluating the ecological effects ofAtrazine:SUPPLEMENTAL CLASSIFICATION (Studies Used for Cosm Endpoint Selection): 1. Berard, A., T. Pelte, et al. (1999a). Seasonal variations inthe sensitivity ofLake Geneva phytoplankton community structure to atrazine. Archiv Fur Hydrobiologie 145(3): 277- 295. (MRID 48261103) 2. Berard, A., C. Leboulanger, et al. (1999b). Tolerance ofOscillatorialimnetica Lemmermann to atrazine in natural phytoplankton populations and inpure culture: Influence ofseason and temperature. Archives ofEnvironmental Contamination and Toxicology 37(4): 472-479. (MRID 47543601) 3. Berard, A. and C. Benninghoff(2001). Pollution-induced community tolerance (PICT) and seasonal variations inthe sensitivity ofphytoplanktonto atrazine in nanocosms. Chemosphere 45(4-5): 427-437. (MRID 48261101) 4. Boone, M. D. and S. M. James (2003). Interactions ofan insecticide, herbicide, and natural stressors in amphibian community mesocosms. Ecological Applications 13(3): 829-841. (MRID 48261104) 5. Brockway, D.L., P.D. Smith, and F.E. Stancil. (1984). Fate and effects ofatrazine on small aquatic microcosms. Bull. Environ. Contam. Toxicol. 32:345-353. (MRID 47543602) 6. Carney, C.E. (1983). The effects ofatrazine and grass carp on freshwater communities. Thesis. University ofKansas, Lawrence, Kansas (MRID 47543604) 7. deNoyelles, F. Jr., and W.D. Kettle. (1980). Herbicides inKansas waters
- evaluations of effects ofagricultural runoffand aquatic weed control on aquatic food chains. ContributionNumber 219, Kansas WaterResources Research Institute, University of Kansas, Lawrence, Kansas (MRID 47543605)
8. deNoyelles, F., Jr., W.D. Kettle, and D.E. Sinn. (1982). The responses ofplankton communities in experimental ponds to atrazine, the mostheavily used pesticide inthe United States. Ecology. 63 (5):1285-1293 (MRID 47543607) 9. deNoyelles, F. Jr., and W.D. Kettle. (1983). Site studies to determine the extent and potential impact ofherbicide contamination in Kansas waters. ContributionNumber239, Kansas WaterResources Research Institute, University ofKansas, Lawrence, Kansas. (MRID 47543606)

210. deNovelles, Jr., F., W.D. Kettle, C.H. Fromm, M.F. Moffett, and S.L. Dewey. (1989). Use of Experimental Ponds to Assess the Effects of a Pesticide on the Aquatic Environment. Department of Systematics and Ecology, University of Kansas. Entomological Society ofAmerica. Miscellaneous Publications No. 75: 41

- 56 (MRID 47543608) 11. deNoyelles, F., S.L. Dewey, D.G. Huggins, and W.D. Kettle. (1994). Aquatic mesocosms in ecological effects testing: detecting direct and indirect effects ofpesticides. In: Graney RL, Kennedy J.H., Rodgers J.H. Jr. (Eds.). Aquatic mescocosm studies in ecological risk assessment. Lewis Publishers, BocaRaton, FL. pp. 577-603 (MRID 47543609) 12. Detenbeck, N.E., R. Hermanutz, K. Allen, and M.e. Swift. (1996). Fate and effects of the herbicide atrazine in flow-through wetland mesocosms. Environ. Toxicol. Chern. 15:937-946 (MRID 47543610) 13. Dewey, S.L. (1986). Effects ofthe herbicide atrazine onaquatic insectcommunity structure and emergence. Ecology. 67: 148-162 (MRID 47543611) 14. Diana, S. G., W. J. Resetarits, et al. (2000). Effects ofatrazine onamphibian growth and survival in artificial aquatic communities. Environmental Toxicology and Chemistry 19(12): 2961-2967. (MRID 48261107) 15. Downing, H. F., M. E. Delorenzo, et al. (2004). Effects ofthe agricultural pesticides atrazine, chlorothalonil, and endosulfan on SouthFloridamicrobial assemblages. Ecotoxicology 13(3): 245-260. (MRID 48261110) 16. Gruessner, B. and M.e. Watzin. (1996). Response ofaquatic communities from a Vermont streamto environmentallyrealistic atrazine exposure in laboratory microcosms. Environ. Toxicol. Chern. 15:410-419 (MRID 47543613) 17. Gustavson, K and S. Wangberg. (1995). Tolerance inductionand successionin microalgae communities exposedto copper and atrazine. Aquat. Toxicol. 32:283-302 (MRID 47543614) 18. Hamala, J.A. and H.P. Kollig. (1985). The effects ofatrazine onperiphyton communities in controlled laboratory ecosystems. Chemosphere. 14: 1391-1408 (MRID 47543615) 19. Hamilton, P.B., G.S. Jackson, N.K Kaushik, and KR. Solomon. (1987). The impactof atrazine on lake periphyton communities including carbon uptake dynamics usingtrack autoradiography. Environ. Poll. 46:83-103 (MRID 47543616)

320. Hamilton, P.B., G.S. Jackson, N.K. Kaushik, KR. Solomon, and G.L. Stephenson. (1988). The impactoftwo applications ofatrazine on the plankton communities ofin situ enclosures. Aquat. Toxicol. 13: 123-140 (MRID 47543617) 21. Hamilton, P.B., D.R.S. Lean, G.S. Jackson, N.K. Kaushik, and K.R. Solomon. (1989). The effect oftwo applications ofatrazine on the water quality offreshwater enclosures. Environ. Pollut. 60:291-304 (MRID 47543501) 22. Herman, D., N.K. Kaushik, and KR. Solomon. (1986). Impactofatrazine onperiphyton in freshwater enclosures and some ecological consequences. Can. J. Fish. Aquat. Sci. 43: 1917-1925 (MRID 47543502) 23. Johnson, B.T. (1986). Potential impact ofselected agricultural chemical contaminants on anorthernprairie wetland: A microcosm evaluation. Environ. Toxicol. Chern. 5:473- 485. (MRID 47543503) 24. Jurgensen, T.A. and KD. Hoagland. (1990). Effects ofshort-termpulses ofatrazine on attached algal communities in a small stream. Arch. Environ. Contam. Toxicol. 19:617- 623. (MRID 47543504) 25. Kettle, W.D. (1982). Description and analysis oftoxicant-inducedresponses ofaquatic communities inreplicated experimental ponds. Ph.D. Thesis. University ofKansas, Lawrence, KS. (MRID 48273504) 26. Kettle, W.D., F. deNoyelles, B.D. Heacock, and A.M. Kadoum. (1987). Dietand reproductive success ofbluegill recovered from experimental ponds treated with atrazine. Bull. Environ. Contam. Toxicol. 38:47-52 (MRID 47543506) 27. Knauert, S., B. Escher, et al. (2008). Mixture toxicity ofthree photosystem II inhibitors (atrazine, isoproturon, and diuron) toward photosynthesis offreshwaterphytoplankton studiedin outdoormesocosms. Environmental Science & Technology 42(17): 6424- 6430. (MRID 48261119) 28. Knauert, S., U. Dawo, et al. (2009). Effects ofPhotosystem II Inhibitors and Their Mixture on Freshwater Phytoplankton Succession in OutdoorMesocosms. Environmental Toxicology and Chemistry 28(4): 836-845. (MRID 48261120) 29. Kosinski, RJ. (1984). The effects ofterrestrial herbicides onthe community structure of streamperiphyton. Environ. Pollut. (Series A). 36: 165-189. (MRID 47543507)

430. Kosinki, RJ., and M.G. Merkle. (1984). The effectoffour terrestrial herbicides onthe productivity ofartificial stream algal communities. J. Environ. Qual. 13:75-82. (MRID 47543508) 31. Krieger, K.A., D.B. Baker, and J.W. Kramer. (1988). Effects ofherbicides on stream Aufivuchsproductivity andnutrientuptake. Arch. Environ. Contam. Toxicol. 17:299- 306. (MRID 47543509) 32. Lampert, W., W. Fleckner, E. Pott, U. Schober, and K.U. Starkel. (1989). Herbicide effects onplanktonic systems ofdifferent complexity. Hydrobiologia. 188/189:415-424. (MRID 47543511) 33. Leboulanger, C., F. Rimet, et al. (2001). Effects ofatrazineand nicosulfuron on freshwater microalgae. Environment International 26(3): 131-135. (MRID 48261121) 34. Lynch, T.R., H.E. Johnson, and WJ. Adams. (1985). Impactofatrazine and hexachlorobiphenyl onthe structure and function ofmodel stream ecosystems. Environ. Toxicol. Chern. 4:399-413. (MRID 47543512) 35. McGregor, E. B., K. R. Solomon, et al. (2008). Effects ofplanting systemdesign onthe toxicological sensitivity ofMyriophyllum spicatum andElodea canadensis to atrazine. Chemosphere 73(3): 249-260. (MRID 48261124) 36. Moorhead, D.L. and RJ. Kosinski. (1986). Effectofatrazine onthe productivityof artificial stream algal communities. Bull. Environ. Contam. Toxicol. 37:330-336. (MRID 47543513) 37. Pratt, J.R, N.J. Bowers, B.R Niederlehrer, and J. Cairns, Jr. (1988). Effects ofatrazine onfreshwater microbial communities. Arch. Environ. Contam. Toxicol. 17:449-457. (MRID 47543514) 38. Relyea, R. A. (2009). A cocktail ofcontaminants: how mixtures ofpesticides at low concentrations affect aquatic communities. Oecologia 159(2): 363-376. (MRID 48261129) 39. Rohr, 1. R. and P. W. Crumrine (2005). Effects ofan herbicide and an insecticide on pond community structure and processes. Ecological Applications 15(4): 1135-1147. (MRID 48261130) 40. Rohr, J. R, A. M. Schotthoefer, et al. (2008). Agrochemica1s increase trematode infections in a declining amphibian species. Nature 455(7217): 1235-1239. (MRID 48261131) 541. Seguin, F., J. C. Druart, et al. (2001 a). Effects ofatrazine and nicosulfuron on periphytic diatom communities in freshwater outdoor lentic mesocosms. Annales De LimnologieInternational Journal ofLimnology 37(1): 3-8. (MRID 48273501) 42. Seguin, F., C. Leboulanger, et al. (2001b). Effects ofatrazine and nicosulfuron on phytoplankton in systems ofincreasing complexity. Archives ofEnvironmental Contamination and Toxicology 40(2): 198-208. (MRID 48261134) 43. Seguin, F., F. Le Bihan, et al. (2002). A risk assessment ofpollution: induction of atrazine tolerance inphytoplankton communities in freshwater outdoor mesocosms, using chlorophyll fluorescence as an endpoint. Water Research 36(13): 3227-3236. (MRID 48261133) 44. Stay, F.S., Larsen, D.P., Katko, A, and Rohm, C.M., (1985) Effects ofAtrazine on Community Level Responses in Taub Microcosms. Validation and Predictability of Laboratory Methods for Assessing the Fate and Effects ofContaminants in Aquatic .E;cosystems, ATSM STP 865, T.P. Boyle, Ed., American Society for Testing and Materials, Philadelphia, pp. 75-90 (MRID 45087419)
45. Stay, E.F., A. Katko, C.M. Rohm, M.A Fix, and D.P. Larsen. (1989). The effects of atrazine onmicrocosms developed from four natural plankton communities. Arch. Environ. Contam. Toxicol. 18:866-875. (MRID 47543515)
46. Van den Brink, P.J., E. van Donk, R. Gylstra, S.J.H. Crum, and T.C.M. Brock. (1995). Effects ofchronic low concentrations ofthe pesticides chlorpyrifos and atrazine in indoor freshwater microcosms. Chemosphere. 31 :3181-3200. (MRID 47543516)
INVALID CLASSIFICATION (Studies NotUsed for Cosm Endpoint Selection): 47. Berard, A., T. Pelte, et al. (1998). Characterization ofphytoplankton from two limnic systems contaminated by a herbicidal photosynthetic inhibitor. The PICTmethod (Pollution-Induced Community Tolerance): application and significance. Annales De Limnologie-International Journal ofLimnology 34(3): 269-282. (MRID 48273502) 48. Berard, A, U. Dorigo, et al. (2003). Comparison ofthe ecotoxicological impact ofthe triazines Irgarol 1051 and atrazine onmicroalgal cultures and natural microalgal communities in Lake Geneva. Chemosphere 53(8): 935-944. (MRID 48261102) 49. Bouldin, J. L., J. L. Farris, et al. (2005). Evaluated fate and effects ofatrazine and lambda-cyhalothrin in vegetated and unvegetated microcosms. Environmental Toxicology 20(5): 487-498. (MRID 48261105)

650. Carder, J.P. and K.D. Hoagland. (1998). Combined effects ofalachlor and atrazine on benthic algal communities in artificial streams. Environ. Toxicol. Chern. 17(7): 1415- 1420. (MRID 47543603) 51. Fairchild, J.F., T.W. LaPoint, and T.R. Schwarz. (1994). Effects ofaherbicide and insecticide mixture in aquatic mesocosms. Arch. Environ. Contam. Toxicol. 27:527-533 (MRID 47543612) 52. Juttner, 1., A. Peither, J.P. Lay, AI. Ketrup, and SJ. Ormerod. (1995). An outdoor mescosm study to assess ecotoxicological effects ofatrazine on anatural plankton community. Arch. Environ. Contam. Toxicol. 29:435-441. (MRID 47543505) 53. Lakshminarayana, J.S.S., HJ. O'Neill, S.D. Jonnavithula, D.A. Legerand, P.H. Milburn. (1992). Impact ofatrazine-bearing agricultural tile drainage discharge on planktonic drift ofanatural stream. Environ. Pollut. 76:201-210. (MRID 47543510) 54. DeLorenzo, M. E., J. Lauth, et aI. (1999). Atrazine effects onthe microbial food web in tidal creekmesocosms. Aquatic Toxicology46(3-4): 241-251. (MRID 48261106)

55. Dorigo, D. and C. Leboulanger (2001). A pulse-amplitude modulated fluorescence-based method for assessing the effects ofphotosystem II herbicides on freshwaterperiphyton. Journal ofApplied Phycology 13(6): 509-515. (MRID 48261108) 56. Dorigo, D., X. Bourrain, et al. (2004). Seasonal changes in the sensitivity ofriver microalgae to atrazine and isoproturon along a contamination gradient. Science ofthe Total Environment 318(1-3): 101-114. (MRID 48261109) 57. Graymore, M., F. Stagnitti, et aI. (2001). Impacts ofatrazine in aquatic ecosystems. Environment International 26(7-8): 483-495. (MRID 48261111) 58. Guasch, H., 1. Munoz, et al. (1997). Changes in atrazine toxicity throughout succession of streamperiphyton communities. Journal ofApplied Phycology 9(2): 137-146. (MRID 48261115) 59. Guasch, H., N. Ivorra, et aI. (1998). Community composition and sensitivity of periphytonto atrazine in flowing waters: the role ofenvironmental factors. Journal of Applied Phycology 10(2): 203-213. (MRID 48261113) 60. Guasch, H. and S. Sabater (1998). Lighthistory influences the sensitivityto atrazine in periphytic algae. Journal ofPhycology 34(2): 233-241. (MRID 48261116)

761. Guasch, H., W. Admiraal, et al. (2003). Contrasting effects oforganic and inorganic toxicants on freshwater periphyton. Aquatic Toxicology 64(2): 165-175. (MRID 48261112) 62. Guasch, H., V. Lehmann, et al. (2007). Influence ofphosphate on the response of periphyton to atrazine exposure. Archives ofEnvironmental Contamination and Toxicology 52(1): 32-37. (MRID 48261114) 63. Gustavson, K, F. Mohlenberg, et al. (2003). Effects ofexposure duration ofherbicides onnatural streamperiphyton communities and recovery. Archives ofEnvironmental Contamination and Toxicology 45(1): 48-58. (MRID 48261117) 64. Hoagland, K D., R. W. Drenner, et al. (1993). Fresh-Water Community Responses to Mixtures ofAgricultural Pesticides

- Effects ofAtrazine and Bifenthrin. Environmental Toxicology and Chemistry 12(4): 627-637. (MRID 48261118) 65. Lytle, J. S. and T. F. Lytle (2002). Uptake and loss ofchlorpyrifos and atrazine by Juncus effusus L. in a mesocosm study with amixture ofpesticides. Environmental Toxicology and Chemistry 21(9): 1817-1825. (MRID48261122) 66. Lytle, T. F. and J. S. Lytle (2005). Growth inhibition as indicatorofstress because of atrazine following multiple toxicantexposure ofthe freshwater macrophyte, Juncus effusus L. Environmental Toxicology and Chemistry 24(5): 1198-1203. (MRID 48261123) 67. Munoz, I., M. Real, et al. (2001). Effects ofatrazine on periphyton under grazing pressure. Aquatic Toxicology 55(3-4): 239-249. (MRID 48261125) 68. Navarro, E., H. Guasch, et al. (2002). Use ofmicrobenthic algal communities in ecotoxicological tests for the assessment ofwater quality: the Terriver case study. Journal ofApplied Phycology 14(1): 41-48. (MRID 48261126) 69. Nelson, K J., K. D. Hoagland, et al. (1999). Chronic effects ofatrazine ontolerance ofa benthic diatom. Environmental Toxicology and Chemistry 18(5): 1038-1045. (MRID 48261127) 70. Nystrom, B., M. Paulsson, et al. (2000). Evaluation ofthe capacity for development of atrazine tolerance inperiphyton from a Swedish freshwater site as determined by inhibition ofphotosynthesis and sulfolipid synthesis. Environmental Toxicology and Chemistry 19(5): 1324-1331. (MRID 48261128) 71. Rimet, F., M. H. De Lacotte, et al. (1999). Atrazine andNicosulfuron effects on phytoplanktonic communities ofLake ofGeneva. Archives Des Sciences 52(2): 111-122. (MRID 48273503)
872. Schmitt-Jansen, M. and R. Altenburger (2005). Predicting and observing responses of algal communities to photosystem II-herbicide exposure using pollution-induced community tolerance and species-sensitivity distributions. Environmental Toxicology and Chemistry 24(2): 304-312. (MRID 48261132) 73. Van den Brink, P. 1., S. J. H. Crum, et al. (2009). Effects ofaherbicide-insecticide mixture in freshwater microcosms: Risk assessment and ecological effect chain. Environmental Pollution 157(1): 237-249. (MRID 48261135)
All ofthese open literature studies were classifiedby EPA as supplemental orinvalid. The supplemental studies deviated from the guideline requirements for avariety ofreasons so they may notbe used for RQ calculations (see Bibliography Document, 2011); however, the studies are scientificallyvalid, and, thus all canbe used for risk characterizationpurposes. Ifyouhave any questions regarding these data evaluationrecords, please do nothesitate to contactus.

9