Page 1 of 4 

## STUDY SPECIFIC SUPPLEMENT

To General Study Plan: 90 8- 25

D

- erythro

- hex

- 2

- enonic acid, .gamma.

- lactone, monosodium

salt [CAS RN 6381

- 77

- 7] :

Algal Growth Inhibition Test

Study Director: H Vryenhoef

Test Facility: Harlan Laboratories Ltd.

Shardlow Business Park

Shardlow

Derbyshire

DE72 2GD

UK

Sponsor: Univar USA Inc.

17425 NE Union Hill Road

Redmond

WA 98052

UNITED STATES OF AMERICA

Study Monitor: Greenwich Chemical Consulting, Inc.

3300 PGA Boulevard, Suite 635

Palm Beach Gardens

FL 33410

UNITED STATES OF AMERICA

Harlan Study Number: 41401175

Proposed Schedule:

Experimental Starting Date: 30 April 2014

Experimental Completion Date: 31 May 2014

Draft Report Date: 30 June 2014Harlan Study Number: 41401175 Study Specific Supplement Page 2



### Test Item

Information as provided by the Sponsor.

Identification: D

- erythro

- hex

- 2

- enonic acid, .gamma.

- lactone, monosodium salt

[CAS RN 6381-77-7]

This study is being performed based on the U.S. Toxic Substances Control Act (TSCA) Section

4 Test Rule and the regulations set forth under 40 CFR 799.5087.



### Special Conditions

Study specific parameters:

GSP Section 1.1 Guidelines / Regulations:

The study will be conducted according to the following internationally accepted guidelines and

recommendations:

• OECD Guidelines for Testing of Chemicals (2006) No 201 * "Freshwater Alga and

Cyanobacteria, Growth Inhibition Test"

• Method C.3 of Commission Regulation (EC) No. 761/2009

GSP Section 3.2 Test System:

The study will be conducted using Pseudokirchneriella subcapitata

GSP Section 3.6 Experimental Conditions:

Incubation of the test vessels will be in an INFORS Multitron ® Version 2 incubator

If measured concentrations are ± 20% of the nominal concentrations, results will be based on

nominal concentrations. If measured concentrations are not ± 20% of nominal concentrations

, results will be based on geometric mean measured concentrations.

The study report will provide a detailed description of the analytical method that will be

developed pr ior to and utilized during the test.

All elements described in item 61 of OECD 20 1 will be included in the final test report.

* Annex 5 corrected 28 July 2011Harlan Study Number: 41401175 Study Specific Supplement Page 3



## GENERAL INFORMATION

Distribution List

The original s tudy s pecific s upplement will be retained in the study file. Copies of the s tudy

s pecific s upplement and g eneral s tudy p lan along with any amendments will be distributed as

follows:

For the Sponsor: John V. Massingale

Greenwich Chemical Consulting, Inc.

Quality Assurance: Harlan Laboratories Ltd, Shardlow, UKHarlan Study Number: 41401175 Study Specific Supplement Page 4



## SIGNATURES

Study Director: H Vryenhoef

.................................................. Date:

Test Facility

Management: C Mead

.................................................. Date:

For the Sponsor: John V. Massingale

Greenwich Chemical Consulting, Inc.

................................................. Date:

Note: These signatures also acknowledge the procedures described in the corresponding G eneral

S tudy P lan

.