## Data Evaluation Record on the Acute Toxicity ofTebufenozide (Confir" m 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence

------------------------ =EP::...;.A MRID Number 485330-01 Data Requirement: EPA DP Barcode: OECD Data Point: EPA MRID: EPA Guideline: 0392144 liA 8.12 (TGAI) and IliA 10 .8. 1.1 (EP) 485330-01 850.4 100
Test material: Tebu fenozide technical endproduct (Confirm 2F) Common name Purity : 22.84% w/w Chemical name: IUPAC CAS name: 3,5-dimethylbenzoic acid 1-(l,l

-dimethylethyl)

-2

-(4-ethylbenzoyl)hydrazide CAS No.: 112410-23-8 Synonyms Primary Reviewer: Joan Gaidos Signature: Senior Scientist, Cambridge Environmental, Inc. Date: 3/1 5/12 Primary Reviewer: Andrew Sayer

## ~~**_k~ (**  Date: 5/10 /12 EP A/OPP/EFED/ERB4 Secondary Reviewer(s): Thomas Steeger,

# Ph**.D~** 

# $}y Date: 5/ 10 /1 2 EP A/OPP/EFED/ERB4 EPA PC Code 129026 Date Evaluation Completed: 5/10 / 12 CITATION: Porch, J. 2011. Confirm 2F TEP : Toxicity Effects on the Seedling Emergence ofTen Species ofPlants. Unpub lished study performed by W ildlife International, Ltd., Easton, Maryland. Stud) ProjectNumber: 437-120. Study sponsored by Nippon Soda Co., Ltd
. , Chiyoda-ku, Tokyo, Japan. Study completed July 

### 7, 20 

### II. EXECUTIVE SUMMARY: The effect oftebufenozide technical endproduct (Confirm 2F TEP; 22 .84% active ingredien1) onthe seedling emergence ofmonocotyledenous (corn, Zea mays; onion, Allium cepa; ryegrass, Lolium perenne; and wheat, Triticum aestivum) and dicotydenous (radish, Raphanus sativus; cabbage, Brassica oleracea; lettuce, Lactucasativa; sugarbeet, Beta vulgaris; soybean, Glycine max; and tomato, Lycopersicon esculentum) crops was studied at a nor:.tinal applica tion rates of 0 (negative control), 9.6, and 1.91 1bs active ingredient/A (lbs ai/A maximum label rate)
. The measured concentration for onion and tomato was <LOQ (<0
. 18, control), 1.15 and 1
.95 lbs ai/A. The measured concentration for all others was <LOQ (<0.18, control), 0.98 and 1.94 1 bs ailA
. On day 21, the surviving plants per pot were recorded and cut at soil level for measuring the plant height and dry weight. Emergence, survival, shoot height, and dry weight per plant were not affected endpoint <; fo r any species tested
. The% inhibition in seedling emergence in the treated species as compared to the control ranged fro m - 18 to 14% none of which were statistically different (a=0.05) from their respective controls. The most sensitive monocot and dicot species could not be determined due to lack of a s ;gnificant effect of the test material, resulting in overall NOAEC and EC25 values for most crops was 1.94 and> 1
.94 lbs ai/A, which is equivalent to 8.51 and > 8.51 lb TEP/A. The NOAEC and EC25 values for onion and tomato were 1 .95 ;md > 1.95 lbs ai/A which is equivalent to 8.55 and >8.55 lb TEP/A. Page I of 17Data Evaluation Re c: ord on the Acute Toxicity of Tebufenozide (Confirm 2F TEP) to Terrestrial Vascular Plants : Seedling Emergence EPA MRID Number 485330 - 01 Phytotoxic effects observ e d i n the treatment group tested included necrosis , chlorosis, and leaf curl. These effects did not appear to be trea t ment rel i.l te d. Maximum Labeled Rate : I.9 l lb ai/A Results Synopsis Monocot EC 5 o/ IC 50 : >1.94 / 1.9 5 lbs a i / A EC z s / IC 25 : > 1.94/1.9 :5 lbs a il A ECos / lC 05 : > 1. 94 / 1.9 :5 lbs a i / A NOAEC: 1.94 / 1.95 lbs a i/ A 95% C.l.: N / A 95% C. l. : N / A 95% C.I.: N / A Slope: N / A 95% C.l.: N / A Most sensitive monocot: Co u ld not be determined Most sensitive parameter: Could not be determined Dicot ECs of iC so: >1.94 / 1.9 :5 lbs a i/A EC z s / IC 25 : > 1.94 / 1.9 :5 lbs ail A EC 05 / IC 05 : > 1. 94/1.9 5 lbs a i / A NOAEC : 1.94 / 1.95 lbs a i/ A 95% C.I.: N / A 95% C.l.: N / A 95% C . I.: N / A Slope: N / A 95% C.l. : N / A Most sensitive dicot: Co u ld n o t be determined Most sensitive parameter: C o uld not be determ i ned This t oxicity study is clas s ified as ACCEPT ABLE and is consisten t with the guideline recommendations for a Tier I seedling emergence toxic i ty study . I. MATERIALS AND M ETH O DS GUIDELINE FOLLOW E D: This study was conducted using methods based on procedures specified in the U . S . Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines OPPTS Number 850.4 1 00 and OECD Guideline 208. Devia t ions froth OPPTS 850.4100 were noted: I . T h e physico-chemica l properties of the test material were not reported. COMPLIANCE: T h is deviation did not have an impact on the acceptabi l ity of the study. Signed and dated GL P , Quality Assurance and Da t a Confiden t iality statements

were provided. This study was conduc t ed in compliance with the Good Laboratory Practice Standards as published by the U.S. Environmental Protection Agency , 40 CFR Part 160 (1989); OECD Pri n cip l es of GLP (ENY / MC / CHEM (98) 17); and Japan MAFF (II NohSan , Notification No. 6283, Agricu l tura l Production Bureau , 1999), with the following exception: • Periodic ana l yses of well water and soil for potential contaminants were not performed in accordance with GLP standards, but were performed using a certified laboratory and standard U.S. EPA Page 2 of 17

## Data Evaluation Record on the Acute Toxicity ofTebufenozide (Confirm 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence

A
. MATERI ALS: l. Test Material Description: Lot No./Batch No. : Purity: Stability of compound under test conditions:

Storage conditions of test chemicals: analytical methods.

Tebufenozide technical endproduct (Confmn 2F; TEP) Liquid 

### HIIALOOl (Lot no.) 22.84%w /w Analytical verification performed on control and treatment so lution samples collected prior to and following application (Ju ne 29, 2011) yielded an recoveries of93- l 01% ofthe nominal test concen1 r ation. No further analysis was conducted on subsequent dates, and the stability ofthe test material could not be verified. (OECD recommends chemical stability in water and light)
The test material and analytical standard were stored at room temperature . Table 

### 1 lists the chemical physical properties oftebufenozide
. T 

### bl 1 Ph
. 1/ h . I



### a e 

## . IYStca c emtca properttes ofT b fi "d 

### e u enozt e. Parameter Values Comments Water solubility 0.83 ppm DP Barcode D2260 1 1 Vapor pressure 2.25 X 

### 1 0" 8 

### mm Hg DP Barcode D226011 UV absorption Not reported


# -- pK. Not reported 

## - log K0 w 

### 4.25 DP Barcode D226011
2. Test organism: Monocotyledonous (monocot) species: Com (Zea mays, Poaceae; Jarvis Gol den Prolific), Onion (Allium cepa, Liliaceae; Yellow Granex Hybrid 33), Ryegrass (Lolium perenne, Poaceae; Gator 3 Perennial), and Wheat (Triticum aestivum, Poaceae; Glenn Hard Red Spring); EPA recommend sfour monocots in twofamilies, including corn. Dicotyledonous (dicot) species: Radish (Raphanus sativus, Brassicaceae; Cherr y Belle), Cabbage 

### (Brassica oleracea, Brassicaceae; Late F lat Dutch), Lettuce (Lactuca sativa, Asteraceae; Summertime), Sugarbeet (Beta vulgaris, Chenopodiaceae; Beta 4609R) , Soybean (Glycine max, Page 3 of 17Data Evaluation Re e or d on the Acut e Toxicity of Tebufenozide (Confir m 2F TEP) to Terrestrial Vascula r P l ants: Seedling E mergence EPA MRID Number 485330-01 Fabaceae; Mave r ick) , and T oma to (Lycopersicon escu l entu m, Solanaceae; Ru tge r s); EPA recommends six dicots infour.families, including soybean and a root crop. OECD r ecommen d s a minimum of three species selected for testing, at least one from each of the following categories: Ca tegory 1: ryegr c .1ss, rice. oat , wheat, and sorghum; Category 2: mustard , rape, radish, turnip , and Chinese cabbage; Category 3: vetch, mung bean , red clover, fenugreek, lettuce , and cress. Seed source: w;1ea t ob t ained from Johnn y's Selected Seeds, W i nslow, ME; oni on obtained from Park Seed

Co ., Greenwood , SC; ryegrass, r adish, c abb age, and toma t o obtained from T he Meyer Seed Co . , Bal ti mor e, MD; lettuce obtained fi·om Territorial S eed Co. , Cottage Gro ve, OR ; com obtained fro m New Hope See d C o . , Bon Aqua, TN; s ugarbeet obtained from B etasee d , Inc ., Tangent , OR; a nd soybean ob t ained fr om M issou ri Founda t ion See d s, Columb i a, MO . Pri or seed treat m ent/sterilization: Seeds were not trea te d wit h fungicides, ins ect i c i des, or repellents prior to test initi ation . Historical % g t : rmi n a tion of seed: Com , 1 00 %; onion , 98% ; ryegrass , 90%; wheat, 94%; radish, 85%;

cabbage, 85%; kttuce, 98%; suga rb eet, 97%; soybean, 94%, and toma t o , 80%. S eed sto r age , if any: None reported B . STUDY DESIGN: 1. Experimenta l Cond itions a. Limit test No ne . b. Range-findin g study No ran ge -findin g st udy wa s r eported. c . D efinitive Study T bl 2 E a e : xperimental >arameters - See dl ing E mergence. Pa r ameters Seedling Emergence Deta ils Remarks ----------- - -- ------------- ---- ---- ------ ------ -- - Criteria · - D uration of t h e test 21 days ------ -- -- ------------ --- ------------------ - ------ Recommended test duration is 14-21 days. OECD recommends that the test be terminated no sooner than 14 days after 50 percent of the control seedlings have emerged P age 4 of 17

### Data Evaluation Record on the Acute Toxicity of Tebufenozide (Confirm 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence
Parameters

Number of seeds/plants/species/ replicate

Number ofreplicates Control: Adjuvant control: Treated :

Test concentrations Nominal:

Measured:

Method and interval of analytical verification

LOQ: LOD: 

### EPA MRID Number 485330-01 Seedling Emergence Details

l 0 seeds per replicate

4 N/A 4 

### Remarks Criteria

Ten seedsper replicate should be used. OECD**recommend.~** a minimum of jive seeds planted in each replicate within 24 hours of incorporation of the test substance. All seeds of each species for each test should be of the san··e size class. The seedshould not be imbibed.

Four replicatesper do
. w should be used. OECD recommends a m f nimum of four replicatesper treatment 0 (negative control) , 0.96 and 1.91 lbai/A Five test concentrations should be used with a dose 0 (nega tive control) , 4.20 and 8.36 range of2X or 3Xprogression lb fonnulation/A OECD recommends three concentrations, preforably with application rates equivalent to 0.0 (control), 1.0, Ryegrass, wheat. radish, com, 10.0 and 100 mgsubsta:1ce per kg of oven-driedsoil. sugarbeet. soybean, l ettuce, and cabbage: <LOQ (<0.18, control), 0.98 and 1.94 lbs ail A Onion and tomato: <LOQ (<0.18, controls) , 1.15 and 1.95 lbs ai/ A Samples collected from the contro l and the single treatment level spray mixtures were analyzed concurrently with calibration standards via HPLC with variable wavelength detection (234 nm). 0 .18 lbs ai/A Set at the lowest analytical standard analyzed, 1.0 !Jg ai/ml Page 

### 5 of 17Data Evaluation Re c ; or d on the Acute Toxicity of Tebufenozide (Confirm 2F TEP) to Terrestrial Vasculal' P l an ts : Seedling Emergence EPA MRID Number 485330-01**~~-.mm----a------~----------~~------~--------------------------------~** Par amet ers Adjuvant (type, percen ta ge, if used) Test container (pot) Size/Volume Material: (g lass / polystyrene) Growth facil i ty Method/depth of seedin g Tes t material applicatio n. Application time i nclud i ng the plant growth s ta ge Number of applicationApplication in terval Method of application

Seedling Emergence Details N/A- none was used

16 em diameter; 12 em depth Plastic Greenhouse A template was used to indent the soi l surface. Once the seeds were placed in each hole , t hey were covered by depressing the soil surface. Com , wheat , and soybean were planted at a depth of 20 mm , while all other spec i es were planted at a depth of 6 mm. The test material was applied on the soil surface the same day the seeds were planted .

Nl A; single application Applied using an overhead DeVries Research Trackspr ayer equipped with a moveable spray nozzle suspended 41 em above the soil surface. Page 6 of 17

Remarks Criteria**Non~porous** containers should be used. OECD recommends that non - porous plastic or gla z ed pot be u sed .

### Data Evaluation Record on the Acute Toxicity ofTebufenozide (Confi1 ·m 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence
Parameters

DetaiIs ofsoil used Geographic l ocation Depth ofsoil collection Soil texture pH: %sand %si lt %c lay % organic carbon 

### CEC Moi sture at 1 /3 atm (%)

Details ofnutrient medium, if used Watering regime and schedules Water source/type: Volume app lied: Interval ofapplication: Method of application:
Any pest control method/fertilization , ifused Test condition s Temperature : 

### EPA M RID Number 485330-01 Seedling Emergence Details

NI

A; artificial soil N/A Loamy sand 

### 85 6 9 6 .2 Not reported Not reported Not reported

N/A

Well water from greenhouse. Not reported. Every 2 to 8 days
. The plants were bottom-watered using sub-irrigation trays . None reported.

Page 7 of 17 

### Remarks Cr iteria
Soi l was a mixture o f kaolinite clay, industrial quartz sand, and peat with limestone added. A slow-release fertili2 ·er was added to the soil to provide nutrients e ssential for plant growth
. Organic matter: 1 .2% Soil mixes containing sa"'dy loam. loam, or clay loam soil with no greater tit an 2% organic matter are preferable
. Glass bfads, rock wool, and 100% acid washed sand are not prtferred. OECDprefers the soilt c be sieved (0.5 em) to remove coarse fragments
. C arb
.m content should not exceed 1
. 5% (3% organic ma• ter

) . Fine particles (under 20um) makeup should te between 10 and 20%
. The recommendedpH i s betveen 5.0 and 7.5.

EPA prefers that bottc.. m watering be utilized for seed ling emer gence studies so that the chemical is not leached out of the soil dt tring the test.Data Evaluation Re e ord on the Acute Toxicity of Tebufenozide (Confirm 2F TEP) to Terrestrial Vasc u la l' P l a nts: Seedling Emergence EPA MRID Number 485330-01 Parameters Seed li ng Emergence De tai l s Remarks .................................................. Criteria Photoperiod : 16L:8D EPA prefers that th e cold vs warm loving plants be Light intensity and qual i ty: Artificial lighting was used to tested in two separate gro ups to optimi ze plant supplement natural sunlight. growth.

11.2-16.6 PAR OECD prefers that the temperature, humidity and Relative humidity: 1 1.9-93.1% light co nditions be suitable for maintaining normal g r ow th o f eac h speci e s for the test period. Reference chemica l (if used) N / A Name : Concentrations: Oth er parameter s, if any None 2. Observations : Table 3: Observation P a ra me ters- Seedling Emergence Parameter s Seedling E mergence Detai l s Remarks P arameters measured (e . g ., num ber

- Emergenc e - Survival of ge rmin ated seeds,

**em~rged** - P hytotoxicity seed lin gs, plant h eight, dry we ight -Dry weight

or other endpoints) - He i ght M easurement technique for each Emergence, survival, and phytotoxicity wer e parameter detennined visually . Height was measured with a ruler to the nearest whole centimeter from the so il surface to the tip o f th e

apical meristem or to the tip of the tall es t l eaf. Dry weight was taken afte r the shoots of all l i vin g see dling s in a rep l i cate were dried in an oven and weighed as a group. Ob se rvation intervals Emergence was measured weekly. Survival , Page 8 of 17

### Data Evaluation Record on the Acute Toxicity ofTebufenozide (Confil'm 2F TEP) to Terrestrial Vascular Plants : Seedling Emergence EPA M RID Number 485330-01 

# - phytotoxicity, height, and dry weight were detennined at study tennination. Other observations, ifany None
Were raw data included? Yes Phytotoxicity rating system, ifused 0- No effect; Toxicity rating system based on : Frans, R. E. 10

-30

- Slight effect; and R. E . Talbert. 1977
. Design of Field 40-60

- Moderate effect; Experiments and t he Measurement and Analysis 70-90- Severe effect; ofP lant Responses. Pages 15

-23 in B. Truelove, I00- Complete effect ed. 

### Research Methods in WeedScience. Southern Weed Science S ociety, Auburn University, Alabama
. II. RESULTS and DISCUSSION: A. INHIBITORY EFFECTS: 1. Seedling Emergence: According to the study authors, negative control emergence ranged from 40 to l 

### OOYo. There was no statistical difference in emergence for ryegrass, wheat, com, lettuce, and radish as compared to the negative control (Table 

### 5a). There was promotion in cabbage at the 1
.91 lb ail A treatment level, but inhibition at the 0.96 lb ai/A. Inhibitions 

### in emergence for all other species ranged from 5 to 15%
. Surviva l was defined by the study authors as the number survived of the number emerged. Survival in the negative contro l ranged from 86 to 100% (Table 5b )
. There was no inhibition or promotion of survival for ryegrass, sugarbeet, and cabbage. Inhibitions for all others ranged from 0 to 6%. According to the study authors, there was no inhibition or promotion of hei ght for onion, ryegrass, com, sugarbeet, cabbage, soybean, lettuce, and tomato (Table Sc) In wheat, there was promotion at 0.96lb ai/A and inhibition (1 %) at 1.91 lb ail A
. 

### In radish, there was promotion at 0.96 lb ai/A and inhibition (9%) at 1
.91 lb ailA. Inhibitions for all others ranged from I to 9%. There was no inhibition or promotion ofdry weight per plant for onion, ryegrass, soybean, and toma to (Table Sd). In sugarbeet, there was promotion at 0
.96 lb ail A and inhibition (4%) at 1. 91 lb ail A. In cabbage, there was promotion at 0.96 lb ail A and inhibition 

### (1 %) at l.91 lb ai/A. Inhibitions for all others ranged from 1 to 12%
. Based on the study authors' results, the most sensit ive monocot species could not 

### br: detennined due to a lack of toxicity (although the study authors did not conduct statistical analysis). The most sensitive dicot species was lettuce based on the presence of inhibitions ofgreater than 25% for height and dr y weight per replicate. The study authors reported that the NOAEC was equal to the highest rate tested and that the EC25 and EC 50 values exceeded the highest rate tested. Phytotoxic effects observed in the plants tested included necrosis, chlorosis, and leaf curl. According to the study authors effects observed in the various treatment groups were likely not an effect ofthe test material. Page 9 of 17Data Evaluation

**Re or**  **d** on the Ac u te Toxicity ofTeb u fenozide (Confirm 2F T EP) to Terrestrial Vascula r P l a nts: Seedling E mergence EPA MRID N um ber 485330 - 01 B. REPORTED STATISTICS: E m e r gence, survival, dry .veigh t , and height data were visua ll y a n alyzed using nominal test concentrations. The study authors did not r eport NOAEC o r ECx val u es. T b l 4 M'd d E a e a: I -stu IY **rr~e ce**  Mid-study emergence Co ntrol Onion Whell Com Rye grass Ca bb age Soybean Tomato Lettuce Radish Sugarbeet Form u lation blank 83 60- 1 00 80-1 00 70-100 60-90 70 -100 80-1 00 60-100 50-100 60- 100 40-80 N/A (40-100)T b l 4b PI I . I d a e : ant njury n ex Plant Injury Index * Co ntr o l Onion Wheu Co m Ryegrass Cabbage Soybean Toma t o Le ttu ce Radish Sugarbeet F orm u lation blank 0-13 0-20 0-HI 0 - 9 0 0-1 1 0- 1 0 0-11 0-10 0 - 25 0-25 N/A * 0- No effect: I 0-30- Slight eff e ct ; 4 0-60 - Moderate effect ; 70-90- Severe effect; I 00- Co mplete effect C. V E RI F lCA TIOI\ O F S TATISTICAL RESULTS BY THE R E VI E WER: Analy sis was **conduc ed**  us i ng E x cel ® 2003 and mean-measured test concentrations based on the active ingredient. The neg ati ve control and the sin gl e treatment g r oup were compared using two - s ample t - tests ass umin g equa l varia n ces w h e n differences between the mean values were greate r t h a n 5% (two-tail; a=0.05 ). T b l SS a e ummary o f b

**mo t**  s ens t hve parameters >Y spec t es T able Sa: Emere:ence (%) Tab l e Sb : Survival(% of planted) Species Cnt r l T r e a t. %Diff Treat %Diff Cntrl Treat. %Diff Treat %Diff 0. 96 1 . 91 0.96 1.91 Onio n 90.0 82.5 8 77.5 1 4 93 78 16 75 1 9 Corn 82.5 92 .5 - 12 97 . 5 -18 83 93 - 12 98 -18 Wheat 95.0 97 . 5 -3 95.0 0 95 95 0 95 0 Rye grass 72.5 72.5 0 77.5 -7 73 73 0 78 -7 Sugar beet 65.0 55.0 15 65.0 0 60 53 13 65 -8 Lettuce 77.5 85 . 0 -10 85.0 -10 78 85 -10 83 -6 Cabbage 90.0 77. 5 14 82.5 -8 88 78 11 83 6 Soybean 95.0 90.0 5 95.0 0 95 88 8 95 0 Tomato 90.0 77 . 5 14 77.5 14 90 75 17 78 14 R adish 75.0 85.0 -13 8 5.0 -13 75 80 -7 85 -1 3 T l b l e Sc: Shoot l ength (em) Table Sd: Dry we i h t (me) Species Cntrl T r e a t. %Diff Treat %D i ff C ntr l T r eat. %Diff Treat % Diff 0.96 1.91 0.96 1.91 Onion 1 7.1 17.7 -4 17 .8 -4 24.9 27.1 -9 29.8 -20

Corn 4 9.1 49.1 0 52.4 -7 574 530 8 563 2 Page 10 of 17

### Data Evaluation Record on the Acute Toxicity ofTebufenozide (Confirm 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence EPA MRID Number 485330-01 Wheat 44.7 45 .3 -1 44.2 1 284 260 8 260 8 Ryegrass 13.8 16.0 -16 14.6 -6 23.0 30.2 ·-32 34.4 -6 Sugarbeet 14
. 1 16.0 -13 14.2

- 1 232 285 ·· 23 223 4 Lettuce 10.6 10.7 -1 11.4 -8 146 131 10 140 4 Cabbage 13.6 14
.7 -8 14.8 -9 306 326 -6 304 1 Soybean 15 .6 16.5 -6 16
.6 -6 371 396 -7 383 -3 Tomato 1 4.7 19
.7 -13 17 .8 -2 394 511

- 30 449 -14 Radish 13 .1 13.4

-2 11.9 9 315 277 12 280 II Control emergence ranged from 65.0-95.0%. Treatment emergence ranged from 55 0-97.5% and 65
.0

-97.5% for treatment levels 0
.96 and I .91 lb ailA, respectively
. Emergence ranged from 15% inhib ition t o 18% promoti on. Control survival ranged from 60-95%. Treatment survival ranged from 53-95% and 65-98% f or t reat nent levels 0.96 and l.91lb ailA, respectively
. Survival ranged from 19% inhibition to 18% promotion. Control length ranged from 10.6-49
. 1 em. Treatment length ranged from l0.7-49.lcm and ll.4-52.4cm for treatment levels 0.96 and 1.91 lb ail A, respectively. Lengt h ranged from 9% inhibition to 16% promotion. Control dry weight ranged from 23. 0-574.0 em. Treatment dry weight ranged from 27.1-530 em and 29.8-563 em for treatment levels 0
.96 and 1
.91 lb ail A, respectively. Dry weight ranged from 12% inhibition to 32% promotion. T bl 6 PI I
. I d

a e 

### a: ant n.1ury n 

### ex Plant In jury Index * Control Onion Wheat Com Ryegrass Cabbage Soybean Tomato Lettuce

0-13 0-20 0-10 0-9 0 0-11 0-10 0-11 0-10 

## * 0- No effect; 10-30- Slight effect; 40-60- Moderate effect; 70-90- Severe effect; 100- Complete effecl T bl 6b M'd d E

a e 

### : I -stu IY mergence Mid-study emergence Control Onion Wheat Com

83 60-100 80-100 70-100 (40-100)
Monocot 

### ECsollCso: > 1. 94/ 1.95 lbs ai/A

### * EC2s/IC2s: > 1.94 / 1.95 lbs ai/A* ECosfiCos: > 1.94 / 1.95 lbs ai/A

## * NOAEC: 1.94/1.95 lbs ai/A* Ryegrass 60-90

Slope: N/A 95% C.l.: N/A Cabbage Soybean 70-100 80-100

95% C.l.: N/A 95% C.l.: N/A 95% C.I.: N/A
Most sensitive monocot: Could not be determined Most sensitive parameter: Could not be determined Tomato Lettuce 60-100 50-100 Rad i sh Sugarbeet Formulation blank 0-2

. 5 0-25 N /A

Rad ish Sugarbeet F ormulation

blank 60- : 00 40-80 N/A

*For most crops the EC 5ofiC 50 test concentration was 1 .94 lbs ai/A. For tomato and onion 1 he 

### ECsofiCso concentrat ion was 1.95 Jbs ail A. Dicot ECso/ICso: > 1.94 / 1.951bs ai/A* EC 2sfiC 2

### s: > 1.94 / 1.95 lbs ai/A

## * EC 0s/ IC 05 : > 1.94 /1.95lbs ai/A* 95% C.l.: N/A 

### 95% 

### C.I.: N/A 95% C.l.: N/A

Page 11 of 17Data Evaluation Re e or d on the Acute Toxicity ofTebufenozide (Confirm 2F TEP) to Terrestrial Vascular · P l ants : Seedling Emergence EPA MRID Number 485330-01 NOAEC: 1.94 / 1.95 l b s a il A* Slope: N / A 95% C.l.: N / A Most sensiti ve dicot: Co ul d not be determined Most sensitive parameter: Could not be determined *For most crops th e**EC IC** **0** t est concentration was 1 . 94 lbs a il A. For tomato and onion the EC 50 / IC 50 concentration

was 1.95 lbs ai / A. D . STUDY DEFIC I ENC I ES: There was considerable varia tion in the controls for certain measurement end points which likely affecte . d the availability of the stu d y to det ect treatment effects. This underscores the need to present the actual values and percent of controls fo r e a ch of the plants tested. However , eve n with this deficiency, the data strongly suggests

there was no much o f an e ff ect across the species tested. E. R EVIEWER' S COMMENTS: The reviewer's and t h e s tu dy authors' result s were in agreement. The reviewer had difficulty comparing results, because the study aut h or s did not conduct statistical analysis or report toxicity values. The study authors visually determined tha t ther e wa s no adverse effects on seedling emergence, survival , growth (weight and height), or

condition of the ten t e rre stria l plant species tested. The r e was insufficient data to conduct a regression analysis to

support the calculatio n of a n ECx value; however, empirically, there did not appear to be a treatment response where inhibition was suffici e nt t o support an EC 25 and/or EC 50 . The revi ewer's results based on measured concentrations are presented in the Ex ecut i ve Summary and Conclusions sections of this DER. The study author de fi ne d s urvival as the number survived of the number that emerged. The reviewer calculated survival based on the 11 umber of surviving plants as a proportion of the number planted . The emergence and survival data (based on the re v ie w er's calculations) were not statistically different, indicating that test plants successfully emerged and survive d s im i l ar to controls. Statistically significant differences were not detected for either set of survival data. The in-life phase of t h e study was conducted from March 16 to June 13,2011.

F. CONCLUSIONS : This toxicity study is classi fi ed as ACCEPTABLE. None of the species tested were significantly affected by the technical endproduct o ft e bufenozide up to the maximum rate tested in this study. The most sensitive monocot and dicot species could n o t b e determined due to a lack of an effect of the test material. T he variability in the negative control emergence ra n ged fro m 40-100% making statistical analysis difficult. The resulting overall NOAEC and EC 5 0 values for most c rops was 1.94 and > 1.94 lbs a il A. The NOAEC and EC 50 values for onion and tomato were 1.95 and >1.9 5 lbs ai / A. Most sensitive mono c ot and EC 25 : Could not be determined(> 1.94 / 1.95 lbs ai/A)* Most sensitive dicot a nd EC 25 : Could not be determined( > 1.94 / 1.95 lbs ai / A)* *For most crops the**~st** co ncentration was 1 . 94 lbs ai / A. Fo r tomato and onion the test concentration was 1.95 Jbs ai l A. III . REFERENCES: I. U.S. Environmen t al Pr otection Agency. 1996. Series 850 - Ecological E ffects Test Guidelines (draft), OPP TS Page 12 ofl7

### Data Evaluation Record on the Acute Toxicity of Tebufenozide (Conf irm 2F TEP) to Terrestrial Vascular Plants : Seedling Emergence**--------------------- --** **.;;;;.E~PA~ MlliD**  Number 

### 485330-01 Number 850.4100: Terrestrial Plant Toxicity, Tier I (Seedling Emergence). 2. Frans, Robert E. and Ronald E. Talbert
. 1977. Design ofField Experiments and the M easurement and Analysis of Plant Responses. Pages 15-23 

### in B. Truelove, ed. 

### Research Methods in Weea Science. Southern Weed Science Society, Auburn University, Alabama
. 3. SAS Institute, Inc. 1999. SAS Proprietary Software Version 8, Cary, NC, SAS institute, Inc.

Page 13 of 17Data E valuation R ec ord on t he Acute Toxic i ty o f T ebufenozide ( C onfirm 2F TEP) to T e rr estr ia l Vasc u la :r Pl a n t s: S eed l ing E mergence E P A M RID Number 4 85330-01 APPENDIX I. OU T PUT OF REV I EW E R ' S S T A TI S TICAL V ERIF IC A TI ON : t - T est : T wo-Samp l e A s su mi ng Equal Va ri ances Sugarbeet Emergenc e · Cntrl vs. 0.96 Variable 1 Variable 2 Mean 65 55 Variance 300 300 Observations 4 4 Pooled Variance 300 Hypothes i zed M ean D i ffe r ence 0 df 6

t Stat 0.816496581 P(T <=t) one-tai l 0 . 222707778 t Critical one - tai l 1.943180274 P(T <=t) two-tail 0.445415555 t Crit i ca l two-tail 2.446911846 t-Test : Two - Samp l e A :. su m i ng Equal Var i a n ces Cabbage Emergence Cntrl vs . 0 . 96 Variable 1 Variable 2 Mean 90 77 . 5 Var i ance 66.66666667 91.66666667 Obse r vat i ons 4 4

Poo l ed Variance 79.166666 6 7 Hypothesized Mean D i fference 0 df 6

t Stat 1 .986798536 P(T<=t) one - tai l 0.047066383 t Cr it ical one - ta il 1 . 943180274 P(T<=t) two-tail 0.094132766 t Crit i cal two - tail 2.446911846 P ag e 14 o f 17

## Data Evaluation Record on the Acute Toxicity ofTebufenozide (Confirm 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence

t-Test: Two-Sample Assuming Equal Variances Soybean Emergence 

### Cntrl vs. 0
.96 Variable 1 Variable 2 Mean Variance Observations Pooled Variance Hypothesized Mean Difference df t Stat P(T<=t) one-tail t Critical one-tail P(T<=t) two-tail t Critical two -tail 

### 95 33.33333333 4 

## so

0 6 1 0.177958842 1.943180274 0.355917684 2.446911846 90 66 .66666667 4

Page 15ofl7 EPA MRID Number 485330-01Data Evaluation R c:c o r d on the Acute Toxicity of T ebufenozide (Confirm 2F TEP) to T e rr est ria l Vascula r Plants: Seedli ng Emergence t-Test: Two-Sample A ss um i ng Equa l Variances

Corn We i ght Cntrl vs . 0 . 96 Variable 1 Mean 574 Variance 6143 . 333333 Observat i ons 4

Pooled Var i ance 9011.166667 Hypothesized Mean Difference 0 df 6

t Stat 0.662955683 P(T <=t) one - tail 0.265993271 t Critica l one - tail 1.943180274 P(T < = t) two - tail 0.531986543 t Crit i cal two-tail 2.446911846 t -T est: Two-Sample Assum i ng Equa l Var i ances L ettuce We i ght Cntrl vs. 0.96 Variable 1 Mean 145.75 Var i ance 1454.916667 Obse r vat i ons 4

Poo l ed Va r iance 809.125 Hypothes i zed Mean Difference 0 df 6

t Stat 0. 733329588 P(T <=t) one-ta i l 0 . 24550255 t Critica l one-tai l 1.943180274 P(T<=t) two-tail 0.4910051 t Critica l two-tai l 2.446911846

Variable 2 529.5 11879 4 Variable 2 131 163 . 3333333 4 P age 16 of 17

EPA MRID Number 485330 - 01

## Data Evaluation Record on the Acute Toxicity of Tebufenozide (Confirm 2F TEP) to Terrestrial Vascular Plants: Seedling Emergence

t-Test: Two-Sample Assuming Equal Variances Radish Hei ght Cntrl vs. 1.91 Variable 1 Mean 

### 13.075 Variance 

### 1.1425 Observations 

### 4 Poo led Va riance 

### 1.1725 Hypothesized Mean Difference 

### 0 df 

### 6 t Stat 

### 1.501953241 P{T<=t) one-ta i l 

### 0.091895432 t Critical one-tail 

### 1.943180274 P{T<=t) two-tail 

### 0.183790864 t Critica l two-ta il 

### 2.446911846 Variable 2 

### 11.925 1.2025 4

Page 17 of 17 EPA MR ID Number 485330

-01