PASTEURIA USGAE – LIQUID FORMULATION

Biological control for use on turf and strawberries against sting nematode 

 

Active Ingredient: Pasteuria usgae* ................. 0.0033%

Other Ingredients: ........................................... 99.9967% 

Total: ................................................................. 100.0000% 

* Contains at least 1.3x107 spores per g or 5.8x109 spores per pound. 

KEEP OUT OF REACH OF CHILDREN

CAUTION 

See additional precautionary statements on the back panel 

EPA Registration No: 85004-3 

EPA Est. Number: 70051-CA-001 

 Manufactured for: 

 12085 Research Dr., Suite 185 

Alachua, FL 32615 

(386) 462-0008 

www.pasteuriabio.com/

Net Contents: Batch Code: 
Proposed Label for Pasteuria usgae – Liquid Formulation

PRECAUTIONARY STATEMENTS 

HAZARDS TO HUMANS AND DOMESTIC ANIMALS 

CAUTION 

Harmful if absorbed through skin or inhaled. Avoid contact with skin, eyes, or 

clothing. Avoid breathing spray mist. Wash thoroughly with soap and water after 

handling and before eating, drinking, chewing gum, using tobacco, or using the 

toilet. Remove and wash contaminated clothing before reuse. 

PERSONAL PROTECTIVE EQUIPMENT (PPE) 

Applicators and other handlers must wear: 

• Long-sleeved shirt and long pants 

• Waterproof gloves 

• Shoes plus socks 

Mixers/loaders and applicators must wear a dust/mist filtering respirator meeting 

NIOSH standards of at least N-95, R-95, or P-95. Repeated exposure to high 

concentrations of microbial proteins can cause allergic sensitization. 

Follow the manufacturer’s instructions for cleaning/maintaining PPE. If no such 

instructions are available, use detergent and hot water for washables. Keep and wash 

PPE separately from other laundry. 

Pasteuria Bioscience, Inc. 2 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

ENVIRONMENTAL HAZARDS 

Do not apply directly to water, or to areas where surface water is present or to 

intertidal areas below the mean high water mark. 

Do not contaminate water when cleaning equipment or disposing of equipment wash 

waters or rinsate. 

DIRECTIONS FOR USE 

It is a violation of Federal law to use this product in a manner inconsistent with 

its labeling. 

Do not use this product until you have read the entire label. Do not apply this 

product in a way that will contact workers or other persons, either directly or through 

drift. Only protected handlers may be in the area during application. For any 

requirement specific to your State and Tribe, consult the agency responsible for 

pesticide regulations. 

Pasteuria Bioscience, Inc. 3 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

PRODUCT INFORMATION 

This product contains a biological nematicide for the control of the following 

nematode for most turf grass and strawberry uses: 

Sting Nematode Belonolaimus longicaudatus 

USE RESTRICTIONS 

Consult your extension agent or manufacturing representative before mixing this 

product with fertilizer or seed. Do not mix this product with other strawberry or turf 

grass pesticides. 

APPLICATION INSTRUCTIONS 

Pasteuria Bioscience, Inc. 4 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

Turf Grass: Apply 0.1 to 5 gallons of Pasteuria usgae – Liquid Formulation per 

100 square feet of turf prior to, during, and after turf green-up in 3 to 4 week 

intervals. Use the higher application rates on sites with high sting nematode 

populations (greater than 60 sting nematodes per 0.4 cup [100 cm3] of soil) or areas 

with extensive nematode damage such as bare turf areas, dying turf areas, or turf 

with root systems less than 0.8 – 1.2 in (2 – 3 cm) in length. For best control, a 

minimum of 3 sequential applications is required. For warm-season grasses, these 

applications may be in early spring and again in late summer or early fall; for coolseason grasses, this may be late summer or early fall. Monthly applications 

throughout the year may help maintain sting nematode populations below economic 

threshold levels. 

Pasteuria usgae – Liquid Formulation may be diluted in water to attain proper 

distribution across the turf areas to be treated. This product may be applied using 

standard calibrated ground spray-application equipment or applied via chemigation 

through the existing irrigation system if it is properly designed for such applications. 

Agitation in the mix tank is recommended for any application requiring dilution of 

the formulated product with water prior to application. When mixing, fill the tank 

one-half full with water, add Pasteuria usgae – Liquid Formulation slowly to the 

tank with hydraulic or mechanical agitation, and continue to fill with water. For 

diluted product applications, apply product in the appropriate amount of water to 

treat the root-zone of the turf without flushing the product beyond the root-zone (this 

can vary with soil type and turf-root density/depth). 

Apply 0.10 inch water immediately following all applications to move the Pasteuria

spores into the turf root-zone, except when the product is diluted more than 50% 

with water for the application (such as chemigation). After application of Pasteuria 

usgae – Liquid Formulation and subsequent treatment with water (as needed), 

minimizing irrigation for several weeks following treatment may improve sting 

nematode control. For areas under intense irrigation or experiencing excessive 

rainfall, repeat applications may be necessary to maintain sting nematode 

populations below an economic threshold. 

Strawberry: Apply 0.1 to 5 gallons of Pasteuria usgae – Liquid Formulation per 

100 square feet of strawberry bed prior to planting and subsequently in 3 to 4 week 

intervals. Use the higher application rate on sites with high sting nematode 

populations (greater than 50 sting nematodes per 0.4 cup [100cc] of soil) or areas 

with extensive nematode damage such as stunted plants, dying plants, or heavily 

stunted root systems compared to unaffected plants in the same field. For best 

control, a minimum of 3 sequential applications is required. Monthly applications 

throughout the year may help maintain sting nematode populations below economic 

threshold levels. 

Pasteuria Bioscience, Inc. 5 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

Pasteuria usgae – Liquid Formulation may be diluted in water to attain proper 

distribution across the area to be treated. This product may be applied using standard 

calibrated ground spray-application equipment and incorporated to a 4 inch depth 

prior to bed formation or applied via chemigation through the irrigation system after 

bed formation (pre-planting). Agitation in the mix tank is recommended for any 

application requiring dilution of the formulated product with water prior to 

application. When mixing, fill the tank one-half full with water, add Pasteuria usgae 

– Liquid Formulation slowly to the tank with hydraulic or mechanical agitation, 

and continue to fill with water. For diluted product applications, apply product in the 

appropriate amount of water to treat the root-zone of the crop without flushing the 

product beyond the root-zone of the crop (this can vary with soil type and crop 

stage). 

Apply enough water following all applications to move the Pasteuria spores into the 

strawberry root-zone and to flush the irrigation lines of any remaining product. After 

application of Pasteuria usgae – Liquid Formulation and subsequent treatment 

with water, minimizing irrigation for several weeks following treatment may 

improve sting nematode control. For areas under intense irrigation, repeat 

applications may be necessary to maintain sting nematode populations below an 

economic threshold. 

DAYS TO HARVEST 

There are no restrictions on applying Pasteuria usgae – Liquid Formulation up to 

the time of harvest. 

CHEMIGATION 

General Requirements: 

1) Apply this product only through sprinkler (including center pivot, lateral 

move, end tow, side (wheel) roll, traveler, big gun, solid set, or hand move) 

and drip (trickle) irrigation systems. Do not apply this product through any 

other type of irrigation system 

2) Crop injury or lack of effectiveness can result from non-uniform distribution 

of treated water. 

3) If you have questions about calibration, you should contact State Extension 

Service specialists, equipment manufacturers, or other experts. 

4) Do not connect an irrigation system (including greenhouse systems) used for 

pesticide application to a public water system unless the pesticide labelprescribed safety devices for public water systems are in place. 

5) A person knowledgeable of the chemigation system and responsible for its 

operation, or under the supervision of the responsible person, shall shut the 

system down and make any necessary adjustments should the need arise. 

Requirements for Chemigation Systems Connected to Public Water Systems: 

Pasteuria Bioscience, Inc. 6 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

1) Public water supply means a system for the provision to the public of piped 

water for human consumption if such system has at least 15 service 

connections or regularly serves an average of 25 individuals daily at least 60 

days throughout the year. 

2) Chemigation systems connected to the public water systems must contain a 

functional, reduced-pressure zone (RPZ), backflow preventer or the 

functional equivalent in the water supply upstream from the point of pesticide 

introduction. As an option to the RPZ, the water from the public water system 

should be discharged into a reservoir tank prior to pesticide introduction. 

There shall be a complete physical break (air gap) between the outlet end of 

the fill pipe and the top of the overflow rim of the reservoir tank of at least 

twice the inside diameter of the fill pipe. 

3) The pesticide injection pipeline must contain a functional, automatic, quickclosing check valve to prevent the flow of fluid back toward the injection 

pump. 

4) The pesticide injection pipeline must contain a functional, normally closed, 

solenoid-operated valve located on the intake side of the injection pump and 

connected to the system interlock to prevent fluid from being withdrawn from 

the supply tank when the irrigation system is either automatically or manually 

shut down. 

5) The system must contain functional interlocking controls to automatically 

shut off the pesticide injection pump when the water pump motor stops, or in 

cases where there is no water pump, when the water pressure decreases to the 

point where pesticide distribution is adversely affected. 

6) Systems must use a metering pump, such as a positive displacement injection 

pump (e.g., diaphragm pump), effectively designed and constructed of 

materials that are compatible with pesticides and capable of being fitted with 

a system interlock. 

7) Do not apply when wind speed favors drift beyond the area intended for 

treatment. 

Requirements for Sprinkler Chemigation: 

1) The system must contain a functional check valve, vacuum relief valve, and 

low pressure drain appropriately located on the irrigation pipeline to prevent 

water source contamination from back flow. 

2) The pesticide injection pipeline must contain a functional, automatic, quickclosing check valve to prevent the flow of fluid back toward the injection 

pump. 

3) The pesticide injection pipeline must also contain a functional, normally 

closed, solenoid-operated valve located on the intake side of the injection 

pump and connected to the system interlock to prevent fluid from being 

withdrawn from the supply tank when the irrigation system is either 

automatically or manually shut down. 

4) The system must contain functional interlocking controls to automatically 

shut off the pesticide injection pump when the water pump motor stops. 

Pasteuria Bioscience, Inc. 7 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

5) The irrigation line or water pump must include a functional pressure switch 

which will stop the water pump motor when the water pressure decreases to 

the point where pesticide distribution is adversely affected. 

6) Systems must use a metering pump, such as a positive displacement injection 

pump (e.g., diaphragm pump), effectively designed and constructed of 

materials that are compatible with pesticides and capable of being fitted with 

a system interlock. 

7) Do not apply when wind speed favors drift beyond the area intended for 

treatment. 

Requirements for Drip Chemigation: 

 1) The system must contain a functional check valve, vacuum relief valve and 

 low pressure drain appropriately located on the irrigation pipeline to prevent 

water source contamination from back flow. 

2) The pesticide injection pipeline must contain a functional, automatic, quickclosing check valve to prevent the flow of fluid back toward the injection 

pump. 

3) The pesticide injection pipeline must also contain a functional, normally 

closed, solenoid-operated valve located on the intake side of the injection 

pump and connected to the system interlock to prevent fluid from being 

withdrawn from the supply tank when the irrigation system is either 

automatically or manually shut down. 

4) The system must contain functional inter-locking controls to automatically 

shut off the pesticide injection pump when the water pump motor stops. 

5) The irrigation line or water pump must include a functional pressure switch 

which will stop the water pump motor when the water pressure decreases to 

the point where pesticide distribution is adversely affected. 

6) Systems must use a metering pump, such as a positive displacement injection 

 pump (e.g., diaphragm pump), effectively designed and constructed of 

 materials that are compatible with pesticides and capable of being fitted with 

 a system interlock. 

Pasteuria Bioscience, Inc. 8 of 9 March 16, 2010
Proposed Label for Pasteuria usgae – Liquid Formulation

Pasteuria Bioscience, Inc. 9 of 9 March 16, 2010

WARRANTY STATEMENT 

Pasteuria Bioscience, Inc. (PBI) warrants that this product conforms to its label 

description and is suitable for its intended use if stored and used strictly in 

accordance with the Directions for Use under normal conditions of use. To the extent 

consistent with applicable law, PBI makes no warranty, express or implied, of 

merchantability, fitness or otherwise concerning the use of this product other than as 

indicated on the label. User assumes all risks of use, storage or handling not in strict 

accordance with accompanying directions.