# Patent

## Title
Polypeptides, and preparation and DNA encoding thereof

## Abstract

This application is a continuation of PCT Application No. PCT/GB95/02243, filed Sep. 21, 1995, now abandoned.

## Background
The present invention relates to a novel human gp130 protein, DNA sequences coding for this protein, its use in therapy, particularly in in vitro fertilisation, as well as pharmaceutical formulations comprising such a protein.

Successful embryo implantation requires correct development of the pre-implantation embryo, resulting in a hatched blastocyst which is able to implant into receptive endometrium. A considerable body of data has been collected which supports the idea that soluble growth factors, if secreted by the uterine epithelium, act directly on the embryo to control this process (Anderson, E. D., J. Cellular Biochem., 53: 280-287 (1993) and Schultz, G. A. and Hevner, S., Mutat Res., 296: 17-31 (992)).

In addition, developing embryos have been shown to produce a variety of cytokines which may act in an autocrine fashion on the endometrium to influence its receptivity. Examples of growth factors shown to be produced by human embryos include IL-1, IL-6, CSF-1 and TNF-.alpha. (Zolti et al, Fertil. Steril., 56 (1991) 265-272 and Witkin et al, J. Reprod. Immunol., 19 (1991) 85-93). TNF-.alpha. has been shown to be present in culture medium of human embryos up to the morula stage, but not that from the blastocyst (Lachappelle et al, Human Reproduction, 8: 1032-1038 (1993)). Production of cytokines by the embryo may therefore be regulated in a stage-specific manner.

Data on the possible direct effects of cytokines on embryos have come primarily from experiments in mice where many cytokines have been shown to affect the development of preimplantation embryos in vitro. RFN-.gamma. and CSF-1, at physiological concentrations, inhibit the number of embryos developing to the blastocyst stage (Hill et al, J. Immunol. , 139 (1987) 2250-2254). TNF-.alpha. has also been shown to have more subtle effects. Although TNF-.alpha. has no apparent effect on rates of blastocyst formation, it appears to specifically inhibit proliferation of cells contributing to the inner cell mass (ICM), which results in blastocysts with a reduced ICM (Pampfer et al, Endocrinology, 134: 206-212 (1994)).

Other growth factors also have specific effects on ICM cells. For instance, insulin-like growth factors 1 and 2 stimulate ICM proliferation, whereas leukaemia inhibitory factor (LIF) inhibits their differentiation (Harvey et al, Mol. Reprod. Dev., 31 (1992) 195-199).

As mentioned above, IL-6 is one of the growth factors which has been shown to be produced by human embryos. IL-6 is a protein which controls the proliferation and differentiation of many cell types in mammals, and in addition has a role in the control of the immune system. Binding of IL-6 to IL-6R initiates the association of IL-6R with a third component known as gp130, which actually transmits the signal through the cell membrane (Taga et al, PNAS, 89: 10998-11001). gp130 is a transmembrane protein, i.e. it extends through the membrane and projects into the cytoplasm, thus it has distinct domains. In this way, IL-6 "signal" transmission is mediated by means of this protein.

EP-A-0411946 discloses a recombinant gp130 protein, as well as DNA sequences coding for such a protein and methods for its cloning.

Yasukawa et al, Immunology Letters, 31 (1992) 123-130, discloses a soluble, recombinant form of gp130, produced by removing the transmembrane and cytoplasmic regions of the membrane bound form of the protein.

Narazaki et al, Blood., 82, No 4 (1993) 1120-1126, disclosed that soluble forms of gp130 exist and may have potential to inhibit signals normally mediated by transmembrane gp130.

The present invention relates to a novel human gp130 protein, DNA sequences coding for this protein, its use in therapy, particularly in in vitro fertilization, as well as pharmaceutical compositions comprising such a protein.

The invention also relates to an isolated and substantially purified gp130 encoded by a DNA sequence.

The invention further relates to the use of gp130 to antagonize the action of one or more growth factors, wherein the growth factors are one or more of IL-6, LIF, CNTF, Oncostatin M or IL-II.

The invention also relates to the use of gp130 to ensure the correct development of pre-implantation embryos.

The invention further relates to a pharmaceutical composition comprising gp130 together with pharmaceutically acceptable excipients. The invention also relates to the use of this pharmaceutical composition for antagonizing the action of one or more growth factors. The invention further relates to the use of this pharmaceutical composition for ensuring the correct development of pre-implantation embryos.

The invention also relates to a method for antagonizing the action of one or more growth factors in a pre-implantation embryo which comprises the step of administering to the embryo gp130.

There has now been found a novel form of gp130, lacking the transmembrane portion, which form arises by means of alternative splicing of the sequence coding for the transmembrane gp130. This form of gp130 may bind to complexes of, for example, IL-6 receptor, resulting in a blocking of the association of the complex with transmembrane gp130.

The novel splicing pattern, as well as resulting in loss of the transmembrane domain, also results in a frameshift, leading to 45 new amino acids before an in frame stop codon.

Thus, the present invention provides gp130 which includes the following C-terminal sequence (SEQ ID NO:1): - Gln Arg Val Ile Leu Pro Ser Gly His Leu Thr Leu Leu - Gln Gly Thr Ile Leu Ile Gln Lys Ile Lys Cys Ile Gln - Met Ala Ile Ser Leu Met

or a C-terminal sequence which is substantially homologous thereto.

Preferably, the novel gp130 of the invention has the above-noted sequence from amino acid position 614. In one embodiment the novel gp130 of the invention has a sequence at positions 1-613 substantially homologous to that shown in FIG. 2 (SEQ ID NO:6).

At the amino acid level, a protein sequence may be regarded as substantially homologous to another protein sequence if a significant number of the constituent amino acids exhibit homology. At least 40%, 50%, 60%, 70%, 80%, 90%, 95% or even 99%, in increasing order of preference, of the amino acids may be homologous.

Thus, activation of the alternative splicing mechanism can result in the production of a novel gp130 in human blastocysts, resulting in inactivation of the effects of, for example, LIF, which in turn may allow differentiation of the inner cell mass, allowing ICM differentiation to proceed. Clearly, therefore, the novel gp130 of the invention can be used in the treatment of preimplantation embryos to ensure correct differentiation and development prior to implantation in a subject.

In addition, the invention also provides a DNA sequence coding for a protein of the invention which sequence includes a sequence substantially homologous to (SEQ ID NO:2): - CAA AGA GTC ATA TTG CCC AGT GGT CAC CTC ACA CTC CTC - CAA GGC ACA ATT TTA ATT CAA AAG ATC AAA TGT ATT CAG - ATG GCA ATT TCA CTG ATG TAA

"DNA sequence substantially the same" includes all other nucleic acid sequences which, by virtue of the degeneracy of the genetic code, also code for the given amino acid sequence or which are substantially homologous to such a sequence.

Sequences having substantial homology may be regarded as those which will hybridise to the nucleic acid sequence shown in FIG. 2 (SEQ ID NO:5) under stringent conditions (for example, at 35 to 65.degree. C. in a salt solution of about 0.9M)

DNA constructs comprising DNA sequences of the invention form another aspect of the present invention.

As discussed herein, the protein of the invention is useful in antagonising the action of certain growth factors, thus enabling certain development processes to be "switched on" in preimplantation embryos. Thus, in a further aspect, the present invention provides the use of the protein of the invention in antagonising the action of one or more growth factors, including IL-6, Leukaemia Inhibitory Factor (LIF), Oncostatin Myciliary Neurotrophic Factor (CNTF) and IL-II.

In addition, the invention also provides the use of the protein of the invention in the manufacture of a medicament for use in ensuring correct development in preimplantation embryos. Preferably, the medicament is used to ensure that differentiation of the ICM commences at the correct time.

The medicament is preferably presented in the form of a pharmaceutical formulation comprising the protein of the invention together with one or more pharmaceutically acceptable carriers and/or excipients. Such pharmaceutical formulations form a yet further aspect of the present invention.

A final aspect of the present invention provides a method for antagonising the action of one or more growth factors which comprises the step of treating a pre-implantation embryo with the protein of the present invention, preferably in the form of a pharmaceutical formulation. Preferably the invention provides a method for ensuring correct development of a preimplantation embryo.

The invention will now be described by means of the following examples, which examples should not be construed as in any way limiting the present invention. The examples refer to the following figures which show:

FIG. 1: the sequence of the soluble gp130 (SEQ ID NO:4) and a DNA sequence (SEQ ID NO:3) coding for it;

FIG. 2: the sequence of native gp130 (SEQ ID NO:5 and 6);

FIG. 3: agarose gel showing the products of nested RT-PCR amplification on RNA from human embryos. Each panel shows the products of amplification with primers specific for different cDNA targets. Amplified cDNAs from different embryos were loaded in each lane. Lanes are labelled according to cDNA labels in Table 1 (below). Additional samples were: lane p, first trimester trophoblast; lane q, cDNA from 200 BeWO cells; lane r, 10 ng human genomic DNA; and lane s, no input cDNA, as a negative control. DNA molecular weight markers were a 123 base pair ladder loaded in lane i. The sizes of the expected PCR products are shown in bp. ______________________________________ Human embryo cDNAs and controls name stage of development ______________________________________ a   2 cell b 3 cell c 4 cell d 6 cell e 8 cell f monila g blastocyst h culture supernatant for a to g j three pooled blastocysts k culture supernatant for j l 2 .times. 6 cell and 1 .times. 8 cell m culture supernatant for l n 1 .times. 4 cell and 1 .times. 6 cell o culture supernatant for n ______________________________________ samples a to h are from the same donor.

FIG. 4: primers used for RT-PCR (SEQ ID NOS:7 through 14).

Crypopreserved human embryos which had been fertilised as part of an IVF program were used in this study. These embryos had been donated for research purposes by the parents and this study complied with the requirements of the Human Embryology and Fertilisation Authority, and the local ethical committee. Frozen embryos were thawed and cultured in Earles balanced salts medium supplemented with 0.4% human serum albumin (Armour Pharmaceuticals UK), until the required developmental stage, then flash frozen in liquid nitrogen in 5 .mu.l of culture fluid (and thus lysed by ice crystals). An identical volume of culture supernatant was frozen as a control. Any remaining cumulus cells were removed during routine handling.

Total RNA from first trimester trophoblast was isolated by the method of Chomsczynski and Sacchi, Anal. Biochem., 162: 156-159 (1987) in which frozen tissue is homogenised in 5 ml of buffer containing 4 M guanidinium thiocyanate (Gibco BRL Livingston, Scotland), 25 mM sodium citrate pH 7.0, 0.5% sarcosyl and 0.1 M 2-mercaptoethanol. The lysate was acidified by the addition of 0.5 ml of 2 M sodium acetate pH 4, and phenol-chloroform extracted using 5 ml of buffer saturated phenol and 1 ml chloroform-isoamylalcohol (49:1 v/v). The suspension was placed on ice for 15 minutes and centrifuged at 10,000 g for 20 minutes at 4.degree. C. The aqueous phase containing RNA was precipitated, washed twice in 70% ethanol, dried and resuspended in TE (10 mM Tris-HCl pH 7.4 and 1 mM EDTA). The concentration of RNA was determined spectrophotometrically at 260 nm.

RNA was prepared from single human embryos using a scaled down protocol based on the above procedure. To assist precipitation of the RNA 100 .mu.g of carrier yeast tRNA (Gibco BRL, Livingston, Scotland) was added at the homogenisation step. The remaining details are as described above, except that all the volumes were 50 fold less and the whole procedure was carried out in 400 .mu.l Eppendorf tubes.

cDNA was synthesised from half the total RNA from each embryo using AMV reverse transcriptase (Super RT, HT Biotech, Cambridge, UK). 3-5 micrograms of RNA was primed with oligo dT (Pharmacia), according to the manufacturers instructions for 60 minutes at 42.degree. C. PCR amplification of the cDNA preparations was performed as previously described (Sharkey, A. et al, Molecular Endocrinol., 6: 1235-1241 (1992)) with a Hybaid Omnigene DNA thermal cycler in a final volume of 30 .mu.l using 1 U of Taq DNA polymerase (Cetus, Emeryville, Calif.) and 10 .mu.M of each of the pair of external primers (see FIG. 4) in the manufacturer's recommended buffer. The following cycle profile was used: 30s at 95.degree. C., 30s at X.degree. C. , 30s at 72.degree. C. for 30 cycles, where X is the annealing temperature for each pair of cytokine primers. External Primers (.degree. C.) Internal Primers (.degree. C.) ______________________________________ gp130     49            54 HistRNA 52 59 ______________________________________

Oligonucleotide primers

Oligonucleotide primers for gp130 and HistidylRNA synthetase were synthesised on a Cruachem PS250 DNA synthesiser. Primer sequences were designed from published nucleotide sequences (see FIG. 4), such that amplification of any contaminating genomic DNA would result in a differently sized product from the cDNA species.

Because of the small amount of material, two pairs of primers were used for each target cDNA, in a nested PCR protocol. One thirtieth of the cDNA products were amplified using Amplitaq (Cetus), in the manufacturers recommended buffer. Following 30 cycles of PCR using the external primer pair, one fiftieth of the first round reaction was transferred to a fresh tube containing the inner primer pair, and subjected to a further 30 rounds of amplification. As negative control, an equal volume of the culture fluid in which the embryo was grown was extracted and subjected to RT-PCR in the same way. Also, 200 cells of the BeWo cell line (ECACC No 86082803) were extracted as positive control.

The primers used in this study are shown in FIG. 4, together with the size of the expected product. The identity of each product was confirmed by cloning and sequencing as described previously (Sharkey et al, Mol. Endocrinol. (1992)). To ensure that the product detected resulted from amplification of cDNA rather than contaminating genomic DNA, primers were chosen to cross intron/exon boundaries. Ten nanograms of genomic DNA was also subjected to PCR at the same time as the cDNA to verify no product of the expected size resulted from genomic DNA.

RESULTS

The technique of RT-PCR was applied to total RNA extracted from human embryos produced by in vitro fertilisation. Embryos were cultured to the appropriate stage, then quick-frozen in liquid nitrogen. Stored embryos were thawed and total RNA extracted. In order to produce detectable RT-PCR product from total RNA extracted from a single embryo, a nested PCR protocol was employed in which the cDNA was subjected to two sets of PCR amplification with an external primer pair, followed by an internal pair. Primers were based on published cDNA sequences and designed to span intron-exon boundaries so that amplification of contaminating genomic DNA could be readily distinguished from cDNA products.

Initially, cDNA from each embryo was tested with primers for histidyl tRNA synthetase (HistRS) to confirm successful RNA isolation and reverse transcription. The primers used gave rise to weak products of greater than 400 bp from genomic DNA and 110 bp from cDNA derived from HistRS MRNA. Transcripts for Hist RS were detected in MRNA from embryos at all stages of development, as well as in decidua and the choriocarcinoma cell line BeWo, used as positive controls (FIG. 3, lanes p and q respectively). No product was detected in an equal volume of embryo culture supernatant extracted and subjected co RT-PCR in the same way, indicating that there was no contamination of the culture with extraneous CDNA or RNA.

Examples of similar RT-PCR analysis with primers for gp130 are shown in FIG. 3. Stocks of cDNA were reverse transcribed from each RNA sample on two separate occasions and the PCR assays were repeated twice on each cDNA stock. The results are shown in FIG. 3, which displays phe pattern of expression of gp130 during preimDlantation development. The identity of the PCR fragment of the correct size was confirmed by sequencing of the cloned PCR product. In cases where novel sized products were seen, these were also cloned and sequenced.

For gp130, the Predicted fragment is 712 bp. However, during the morula to blastocyst transition, a novel, smaller transcript was detected of approximately 600 bp. This result appeared consistent since, in sample j, which derives from cDNA made from 3 pooled blastocysts, both products were detected simultaneously. Upon cloning and sequencing, the smaller product appeared to arise due to an alternative splicing event which removes the exon encoding the transmembrane domain. The predicted sequence of the novel transcript is shown in FIG. 1. The novel splicing pattern also involves a frameshift, resulting in 45 new amino acids, before an in frame stop codon.

DISCUSSION

Many growth factors have been shown to influence the development of cultured preimplantation mammalian embryos (for review see Anderson, E. D., J. Cellular Biochem., 53: 280-287 (1993) and Schultz, G. A. and Hevner, S., Mutat Res., 296: 17-31 (1992)).

However, there is good evidence for species to species differences in expression of growth factor receptors in preimplantation development. For instance, EGF mRNA is expressed in the pig embryo but has not been found at any stage in mouse preimDlantation embryos (Vaughan et al, Development, 116: 663-669 (1992); Rapolee et al, Science, 241: 1823-1825 (1988); and Watson, A. J. et al, Biol. Reprod., 50: 725-733 (1994)). Therefore the usefulness of these studies to researchers interested in factors controlling human preimplantation development is limited. In addition, the specific growth factors and receptors investigated in such studies frequently have been chosen on an ad hoc basis. Both for ethical and practical reasons, such an approach is not suitable for use with human embryos. We have therefore used a nested RT-PCR method which has allowed us to screen for the expression of growth factor and receptor mRNAs in single human preimplantation embryos. This method has been widely is used over the last few years in other species since it is reliable, sensitive and economical in its use of embryo material.

RT-PCR with primers for Histidyl-tRNA synthetase was used on cDNA samples to confirm that cDNA had been successfully prepared form each embryo RNA sample. cDNA specific for this housekeeping gene was successfully detected in cDNA samples made even from a single 2-cell embryo, indicating that the method was sufficiently sensitive for this study.

We found that during the morula to blastocyst transition, the size of the gp130 PCR product decreased by about 100 bp. Sequencing of the smaller product indicated that it results from a novel splice variant of the gp130 mRNA. This new splice variant lacks the transmembrane domain, and would be expected to produce a novel form of gp130. Soluble gp130 protein has recently been detected in human serum and has been shown to antagonise the action of IL-6 and LIFR. When the cytokines associate with their respective receptors, soluble gp130 is able to bind to this complex, blocking association with membrane bound gp130. The possibility is that selective expression of the novel gp130 in the blastocoel cavity in the late blastocyst stage would inactivate the effects of LIF in preventing differentiation of the linear cell mass, allowing ICM differentiation to proceed. #             SEQUENCE LISTING - -  - - (1) GENERAL INFORMATION: - -    (iii) NUMBER OF SEQUENCES: 14 - -  - - (2) INFORMATION FOR SEQ ID NO:1: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 45 amino - #acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: Not Relev - #ant - -     (ii) MOLECULE TYPE: peptide - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: - - Glu Leu Lys Asn Thr Ser Gly Leu Met Phe Gl - #n Ile Leu Gln Arg Val 1               5   - #                10  - #                15 - - Ile Leu Pro Ser Gly His Leu Thr Leu Leu Gl - #n Gly Thr Ile Leu Ile 20      - #            25      - #            30 - - Gln Lys Ile Lys Cys Ile Gln Met Ala Ile Se - #r Leu Met 35          - #        40          - #        45 - -  - - (2) INFORMATION FOR SEQ ID NO:2: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 138 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: - - GAATTAAAAA ACACATCTGG CCTAATGTTC CAGATCCTTC AAAGAGTCAT AT - #TGCCCAGT     60 - - GGTCACCTCA CACTCCTCCA AGGCACAATT TTAATTCAAA AGATCAAATG TA - #TTCAGATG    120 - - GCAATTTCAC TGATGTAA             - #                  - # - # 138 - -  - - (2) INFORMATION FOR SEQ ID NO:3: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1977 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1974 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: - - ATG TTG ACG TTG CAG ACT TGG GTA GTG CAA GC - #C TTG TTT ATT TTC CTC 48 Met Leu Thr Leu Gln Thr Trp Val Val Gln Al - #a Leu Phe Ile Phe Leu 1               5 - #                 10 - #                 15 - - ACC ACT GAA TCT ACA GGT GAA CTT CTA GAT CC - #A TGT GGT TAT ATC AGT 96 Thr Thr Glu Ser Thr Gly Glu Leu Leu Asp Pr - #o Cys Gly Tyr Ile Ser 20     - #             25     - #             30 - - CCT GAA TCT CCA GTT GTA CAA CTT CAT TCT AA - #T TTC ACT GCA GTT TGT 144 Pro Glu Ser Pro Val Val Gln Leu His Ser As - #n Phe Thr Ala Val Cys 35         - #         40         - #         45 - - GTG CTA AAG GAA AAA TGT ATG GAT TAT TTT CA - #T GTA AAT GCT AAT TAC 192 Val Leu Lys Glu Lys Cys Met Asp Tyr Phe Hi - #s Val Asn Ala Asn Tyr 50             - #     55             - #     60 - - ATT GTC TGG AAA ACA AAC CAT TTT ACT ATT CC - #T AAG GAG CAA TAT ACT 240 Ile Val Trp Lys Thr Asn His Phe Thr Ile Pr - #o Lys Glu Gln Tyr Thr 65                 - # 70                 - # 75                 - # 80 - - ATC ATA AAC AGA ACA GCA TCC AGT GTC ACC TT - #T ACA GAT ATA GCT TCA 288 Ile Ile Asn Arg Thr Ala Ser Ser Val Thr Ph - #e Thr Asp Ile Ala Ser 85 - #                 90 - #                 95 - - TTA AAT ATT CAG CTC ACT TGC AAC ATT CTT AC - #A TTC GGA CAG CTT GAA 336 Leu Asn Ile Gln Leu Thr Cys Asn Ile Leu Th - #r Phe Gly Gln Leu Glu 100      - #           105      - #           110 - - CAG AAT GTT TAT GGA ATC ACA ATA ATT TCA GG - #C TTG CCT CCA GAA AAA 384 Gln Asn Val Tyr Gly Ile Thr Ile Ile Ser Gl - #y Leu Pro Pro Glu Lys 115          - #       120          - #       125 - - CCT AAA AAT TTG AGT TGC ATT GTG AAC GAG GG - #G AAG AAA ATG AGG TGT 432 Pro Lys Asn Leu Ser Cys Ile Val Asn Glu Gl - #y Lys Lys Met Arg Cys 130              - #   135              - #   140 - - GAG TGG GAT GGT GGA AGG GAA ACA CAC TTG GA - #G ACA AAC TTC ACT TTA 480 Glu Trp Asp Gly Gly Arg Glu Thr His Leu Gl - #u Thr Asn Phe Thr Leu 145                 1 - #50                 1 - #55                 1 - #60 - - AAA TCT GAA TGG GCA ACA CAC AAG TTT GCT GA - #T TGC AAA GCA AAA CGT      528 Lys Ser Glu Trp Ala Thr His Lys Phe Ala As - #p Cys Lys Ala Lys Arg 165  - #               170  - #               175 - - GAC ACC CCC ACC TCA TGC ACT GTT GAT TAT TC - #T ACT GTG TAT TTT GTC 576 Asp Thr Pro Thr Ser Cys Thr Val Asp Tyr Se - #r Thr Val Tyr Phe Val 180      - #           185      - #           190 - - AAC ATT GAA GTC TGG GTA GAA GCA GAG AAT GC - #C CTT GGG AAG GTT ACA 624 Asn Ile Glu Val Trp Val Glu Ala Glu Asn Al - #a Leu Gly Lys Val Thr 195          - #       200          - #       205 - - TCA GAT CAT ATC AAT TTT GAT CCT GTA TAT AA - #A GTG AAG CCC AAT CCG 672 Ser Asp His Ile Asn Phe Asp Pro Val Tyr Ly - #s Val Lys Pro Asn Pro 210              - #   215              - #   220 - - CCA CAT AAT TTA TCA GTG ATC AAC TCA GAG GA - #A CTG TCT AGT ATC TTA 720 Pro His Asn Leu Ser Val Ile Asn Ser Glu Gl - #u Leu Ser Ser Ile Leu 225                 2 - #30                 2 - #35                 2 - #40 - - AAA TTG ACA TGG ACC AAC CCA AGT ATT AAG AG - #T GTT ATA ATA CTA AAA      768 Lys Leu Thr Trp Thr Asn Pro Ser Ile Lys Se - #r Val Ile Ile Leu Lys 245  - #               250  - #               255 - - TAT AAC ATT CAA TAT AGG ACC AAA GAT GCC TC - #A ACT TGG AGC CAG ATT 816 Tyr Asn Ile Gln Tyr Arg Thr Lys Asp Ala Se - #r Thr Trp Ser Gln Ile 260      - #           265      - #           270 - - CCT CCT GAA GAC ACA GCA TCC ACC CGA TCT TC - #A TTC ACT GTC CAA GAC 864 Pro Pro Glu Asp Thr Ala Ser Thr Arg Ser Se - #r Phe Thr Val Gln Asp 275          - #       280          - #       285 - - CTT AAA CCT TTT ACA GAA TAT GTG TTT AGG AT - #T CGC TGT ATG AAG GAA 912 Leu Lys Pro Phe Thr Glu Tyr Val Phe Arg Il - #e Arg Cys Met Lys Glu 290              - #   295              - #   300 - - GAT GGT AAG GGA TAC TGG AGT GAC TGG AGT GA - #A GAA GCA AGT GGG ATC 960 Asp Gly Lys Gly Tyr Trp Ser Asp Trp Ser Gl - #u Glu Ala Ser Gly Ile 305                 3 - #10                 3 - #15                 3 - #20 - - ACC TAT GAA GAT AGA CCA TCT AAA GCA CCA AG - #T TTC TGG TAT AAA ATA     1008 Thr Tyr Glu Asp Arg Pro Ser Lys Ala Pro Se - #r Phe Trp Tyr Lys Ile 325  - #               330  - #               335 - - GAT CCA TCC CAT ACT CAA GGC TAC AGA ACT GT - #A CAA CTC GTG TGG AAG 1056 Asp Pro Ser His Thr Gln Gly Tyr Arg Thr Va - #l Gln Leu Val Trp Lys 340      - #           345      - #           350 - - ACA TTG CCT CCT TTT GAA GCC AAT GGA AAA AT - #C TTG GAT TAT GAA GTG 1104 Thr Leu Pro Pro Phe Glu Ala Asn Gly Lys Il - #e Leu Asp Tyr Glu Val 355          - #       360          - #       365 - - ACT CTC ACA AGA TGG AAA TCA CAT TTA CAA AA - #T TAC ACA GTT AAT GCC 1152 Thr Leu Thr Arg Trp Lys Ser His Leu Gln As - #n Tyr Thr Val Asn Ala 370              - #   375              - #   380 - - ACA AAA CTG ACA GTA AAT CTC ACA AAT GAT CG - #C TAT CTA GCA ACC CTA 1200 Thr Lys Leu Thr Val Asn Leu Thr Asn Asp Ar - #g Tyr Leu Ala Thr Leu 385                 3 - #90                 3 - #95                 4 - #00 - - ACA GTA AGA AAT CTT GTT GGC AAA TCA GAT GC - #A GCT GTT TTA ACT ATC     1248 Thr Val Arg Asn Leu Val Gly Lys Ser Asp Al - #a Ala Val Leu Thr Ile 405  - #               410  - #               415 - - CCT GCC TGT GAC TTT CAA GCT ACT CAC CCT GT - #A ATG GAT CTT AAA GCA 1296 Pro Ala Cys Asp Phe Gln Ala Thr His Pro Va - #l Met Asp Leu Lys Ala 420      - #           425      - #           430 - - TTC CCC AAA GAT AAC ATG CTT TGG GTG GAA TG - #G ACT ACT CCA AGG GAA 1344 Phe Pro Lys Asp Asn Met Leu Trp Val Glu Tr - #p Thr Thr Pro Arg Glu 435          - #       440          - #       445 - - TCT GTA AAG AAA TAT ATA CTT GAG TGG TGT GT - #G TTA TCA GAT AAA GCA 1392 Ser Val Lys Lys Tyr Ile Leu Glu Trp Cys Va - #l Leu Ser Asp Lys Ala 450              - #   455              - #   460 - - CCC TGT ATC ACA GAC TGG CAA CAA GAA GAT GG - #T ACC GTG CAT CGC ACC 1440 Pro Cys Ile Thr Asp Trp Gln Gln Glu Asp Gl - #y Thr Val His Arg Thr 465                 4 - #70                 4 - #75                 4 - #80 - - TAT TTA AGA GGG AAC TTA GCA GAG AGC AAA TG - #C TAT TTG ATA ACA GTT     1488 Tyr Leu Arg Gly Asn Leu Ala Glu Ser Lys Cy - #s Tyr Leu Ile Thr Val 485  - #               490  - #               495 - - ACT CCA GTA TAT GCT GAT GGA CCA GGA AGC CC - #T GAA TCC ATA AAG GCA 1536 Thr Pro Val Tyr Ala Asp Gly Pro Gly Ser Pr - #o Glu Ser Ile Lys Ala 500      - #           505      - #           510 - - TAC CTT AAA CAA GCT CCA CCT TCC AAA GGA CC - #T ACT GTT CGG ACA AAA 1584 Tyr Leu Lys Gln Ala Pro Pro Ser Lys Gly Pr - #o Thr Val Arg Thr Lys 515          - #       520          - #       525 - - AAA GTA GGG AAA AAC GAA GCT GTC TTA GAG TG - #G GAC CAA CTT CCT GTT 1632 Lys Val Gly Lys Asn Glu Ala Val Leu Glu Tr - #p Asp Gln Leu Pro Val 530              - #   535              - #   540 - - GAT GTT CAG AAT GGA TTT ATC AGA AAT TAT AC - #T ATA TTT TAT AGA ACC 1680 Asp Val Gln Asn Gly Phe Ile Arg Asn Tyr Th - #r Ile Phe Tyr Arg Thr 545                 5 - #50                 5 - #55                 5 - #60 - - ATC ATT GGA AAT GAA ACT GCT GTG AAT GTG GA - #T TCT TCC CAC ACA GAA     1728 Ile Ile Gly Asn Glu Thr Ala Val Asn Val As - #p Ser Ser His Thr Glu 565  - #               570  - #               575 - - TAT ACA TTG TCC TCT TTG ACT AGT GAC ACA TT - #G TAC ATG GTA CGA ATG 1776 Tyr Thr Leu Ser Ser Leu Thr Ser Asp Thr Le - #u Tyr Met Val Arg Met 580      - #           585      - #           590 - - GCA GCA TAC ACA GAT GAA GGT GGG AAG GAT GG - #T CCA GAA TTC ACT TTT 1824 Ala Ala Tyr Thr Asp Glu Gly Gly Lys Asp Gl - #y Pro Glu Phe Thr Phe 595          - #       600          - #       605 - - ACT ACC CCA AAG TTT GAA TTA AAA AAC ACA TC - #T GGC CTA ATG TTC CAG 1872 Thr Thr Pro Lys Phe Glu Leu Lys Asn Thr Se - #r Gly Leu Met Phe Gln 610              - #   615              - #   620 - - ATC CTT CAA AGA GTC ATA TTG CCC AGT GGT CA - #C CTC ACA CTC CTC CAA 1920 Ile Leu Gln Arg Val Ile Leu Pro Ser Gly Hi - #s Leu Thr Leu Leu Gln 625                 6 - #30                 6 - #35                 6 - #40 - - GGC ACA ATT TTA ATT CAA AAG ATC AAA TGT AT - #T CAG ATG GCA ATT TCA     1968 Gly Thr Ile Leu Ile Gln Lys Ile Lys Cys Il - #e Gln Met Ala Ile Ser 645  - #               650  - #               655 - - CTG ATG TAA              - #                  - # - #       1977 Leu Met - -  - - (2) INFORMATION FOR SEQ ID NO:4: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 658 amino - #acids (B) TYPE: amino acid (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: protein - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: - - Met Leu Thr Leu Gln Thr Trp Val Val Gln Al - #a Leu Phe Ile Phe Leu 1               5 - #                 10 - #                 15 - - Thr Thr Glu Ser Thr Gly Glu Leu Leu Asp Pr - #o Cys Gly Tyr Ile Ser 20     - #             25     - #             30 - - Pro Glu Ser Pro Val Val Gln Leu His Ser As - #n Phe Thr Ala Val Cys 35         - #         40         - #         45 - - Val Leu Lys Glu Lys Cys Met Asp Tyr Phe Hi - #s Val Asn Ala Asn Tyr 50             - #     55             - #     60 - - Ile Val Trp Lys Thr Asn His Phe Thr Ile Pr - #o Lys Glu Gln Tyr Thr 65                 - # 70                 - # 75                 - # 80 - - Ile Ile Asn Arg Thr Ala Ser Ser Val Thr Ph - #e Thr Asp Ile Ala Ser 85 - #                 90 - #                 95 - - Leu Asn Ile Gln Leu Thr Cys Asn Ile Leu Th - #r Phe Gly Gln Leu Glu 100      - #           105      - #           110 - - Gln Asn Val Tyr Gly Ile Thr Ile Ile Ser Gl - #y Leu Pro Pro Glu Lys 115          - #       120          - #       125 - - Pro Lys Asn Leu Ser Cys Ile Val Asn Glu Gl - #y Lys Lys Met Arg Cys 130              - #   135              - #   140 - - Glu Trp Asp Gly Gly Arg Glu Thr His Leu Gl - #u Thr Asn Phe Thr Leu 145                 1 - #50                 1 - #55                 1 - #60 - - Lys Ser Glu Trp Ala Thr His Lys Phe Ala As - #p Cys Lys Ala Lys Arg 165  - #               170  - #               175 - - Asp Thr Pro Thr Ser Cys Thr Val Asp Tyr Se - #r Thr Val Tyr Phe Val 180      - #           185      - #           190 - - Asn Ile Glu Val Trp Val Glu Ala Glu Asn Al - #a Leu Gly Lys Val Thr 195          - #       200          - #       205 - - Ser Asp His Ile Asn Phe Asp Pro Val Tyr Ly - #s Val Lys Pro Asn Pro 210              - #   215              - #   220 - - Pro His Asn Leu Ser Val Ile Asn Ser Glu Gl - #u Leu Ser Ser Ile Leu 225                 2 - #30                 2 - #35                 2 - #40 - - Lys Leu Thr Trp Thr Asn Pro Ser Ile Lys Se - #r Val Ile Ile Leu Lys 245  - #               250  - #               255 - - Tyr Asn Ile Gln Tyr Arg Thr Lys Asp Ala Se - #r Thr Trp Ser Gln Ile 260      - #           265      - #           270 - - Pro Pro Glu Asp Thr Ala Ser Thr Arg Ser Se - #r Phe Thr Val Gln Asp 275          - #       280          - #       285 - - Leu Lys Pro Phe Thr Glu Tyr Val Phe Arg Il - #e Arg Cys Met Lys Glu 290              - #   295              - #   300 - - Asp Gly Lys Gly Tyr Trp Ser Asp Trp Ser Gl - #u Glu Ala Ser Gly Ile 305                 3 - #10                 3 - #15                 3 - #20 - - Thr Tyr Glu Asp Arg Pro Ser Lys Ala Pro Se - #r Phe Trp Tyr Lys Ile 325  - #               330  - #               335 - - Asp Pro Ser His Thr Gln Gly Tyr Arg Thr Va - #l Gln Leu Val Trp Lys 340      - #           345      - #           350 - - Thr Leu Pro Pro Phe Glu Ala Asn Gly Lys Il - #e Leu Asp Tyr Glu Val 355          - #       360          - #       365 - - Thr Leu Thr Arg Trp Lys Ser His Leu Gln As - #n Tyr Thr Val Asn Ala 370              - #   375              - #   380 - - Thr Lys Leu Thr Val Asn Leu Thr Asn Asp Ar - #g Tyr Leu Ala Thr Leu 385                 3 - #90                 3 - #95                 4 - #00 - - Thr Val Arg Asn Leu Val Gly Lys Ser Asp Al - #a Ala Val Leu Thr Ile 405  - #               410  - #               415 - - Pro Ala Cys Asp Phe Gln Ala Thr His Pro Va - #l Met Asp Leu Lys Ala 420      - #           425      - #           430 - - Phe Pro Lys Asp Asn Met Leu Trp Val Glu Tr - #p Thr Thr Pro Arg Glu 435          - #       440          - #       445 - - Ser Val Lys Lys Tyr Ile Leu Glu Trp Cys Va - #l Leu Ser Asp Lys Ala 450              - #   455              - #   460 - - Pro Cys Ile Thr Asp Trp Gln Gln Glu Asp Gl - #y Thr Val His Arg Thr 465                 4 - #70                 4 - #75                 4 - #80 - - Tyr Leu Arg Gly Asn Leu Ala Glu Ser Lys Cy - #s Tyr Leu Ile Thr Val 485  - #               490  - #               495 - - Thr Pro Val Tyr Ala Asp Gly Pro Gly Ser Pr - #o Glu Ser Ile Lys Ala 500      - #           505      - #           510 - - Tyr Leu Lys Gln Ala Pro Pro Ser Lys Gly Pr - #o Thr Val Arg Thr Lys 515          - #       520          - #       525 - - Lys Val Gly Lys Asn Glu Ala Val Leu Glu Tr - #p Asp Gln Leu Pro Val 530              - #   535              - #   540 - - Asp Val Gln Asn Gly Phe Ile Arg Asn Tyr Th - #r Ile Phe Tyr Arg Thr 545                 5 - #50                 5 - #55                 5 - #60 - - Ile Ile Gly Asn Glu Thr Ala Val Asn Val As - #p Ser Ser His Thr Glu 565  - #               570  - #               575 - - Tyr Thr Leu Ser Ser Leu Thr Ser Asp Thr Le - #u Tyr Met Val Arg Met 580      - #           585      - #           590 - - Ala Ala Tyr Thr Asp Glu Gly Gly Lys Asp Gl - #y Pro Glu Phe Thr Phe 595          - #       600          - #       605 - - Thr Thr Pro Lys Phe Glu Leu Lys Asn Thr Se - #r Gly Leu Met Phe Gln 610              - #   615              - #   620 - - Ile Leu Gln Arg Val Ile Leu Pro Ser Gly Hi - #s Leu Thr Leu Leu Gln 625                 6 - #30                 6 - #35                 6 - #40 - - Gly Thr Ile Leu Ile Gln Lys Ile Lys Cys Il - #e Gln Met Ala Ile Ser 645  - #               650  - #               655 - - Leu Met - -  - - (2) INFORMATION FOR SEQ ID NO:5: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2754 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..2754 - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: - - ATG TTG ACG TTG CAG ACT TGG GTA GTG CAA GC - #C TTG TTT ATT TTC CTC 48 Met Leu Thr Leu Gln Thr Trp Val Val Gln Al - #a Leu Phe Ile Phe Leu 1               5 - #                 10 - #                 15 - - ACC ACT GAA TCT ACA GGT GAA CTT CTA GAT CC - #A TGT GGT TAT ATC AGT 96 Thr Thr Glu Ser Thr Gly Glu Leu Leu Asp Pr - #o Cys Gly Tyr Ile Ser 20     - #             25     - #             30 - - CCT GAA TCT CCA GTT GTA CAA CTT CAT TCT AA - #T TTC ACT GCA GTT TGT 144 Pro Glu Ser Pro Val Val Gln Leu His Ser As - #n Phe Thr Ala Val Cys 35         - #         40         - #         45 - - GTG CTA AAG GAA AAA TGT ATG GAT TAT TTT CA - #T GTA AAT GCT AAT TAC 192 Val Leu Lys Glu Lys Cys Met Asp Tyr Phe Hi - #s Val Asn Ala Asn Tyr 50             - #     55             - #     60 - - ATT GTC TGG AAA ACA AAC CAT TTT ACT ATT CC - #T AAG GAG CAA TAT ACT 240 Ile Val Trp Lys Thr Asn His Phe Thr Ile Pr - #o Lys Glu Gln Tyr Thr 65                 - # 70                 - # 75                 - # 80 - - ATC ATA AAC AGA ACA GCA TCC AGT GTC ACC TT - #T ACA GAT ATA GCT TCA 288 Ile Ile Asn Arg Thr Ala Ser Ser Val Thr Ph - #e Thr Asp Ile Ala Ser 85 - #                 90 - #                 95 - - TTA AAT ATT CAG CTC ACT TGC AAC ATT CTT AC - #A TTC GGA CAG CTT GAA 336 Leu Asn Ile Gln Leu Thr Cys Asn Ile Leu Th - #r Phe Gly Gln Leu Glu 100      - #           105      - #           110 - - CAG AAT GTT TAT GGA ATC ACA ATA ATT TCA GG - #C TTG CCT CCA GAA AAA 384 Gln Asn Val Tyr Gly Ile Thr Ile Ile Ser Gl - #y Leu Pro Pro Glu Lys 115          - #       120          - #       125 - - CCT AAA AAT TTG AGT TGC ATT GTG AAC GAG GG - #G AAG AAA ATG AGG TGT 432 Pro Lys Asn Leu Ser Cys Ile Val Asn Glu Gl - #y Lys Lys Met Arg Cys 130              - #   135              - #   140 - - GAG TGG GAT GGT GGA AGG GAA ACA CAC TTG GA - #G ACA AAC TTC ACT TTA 480 Glu Trp Asp Gly Gly Arg Glu Thr His Leu Gl - #u Thr Asn Phe Thr Leu 145                 1 - #50                 1 - #55                 1 - #60 - - AAA TCT GAA TGG GCA ACA CAC AAG TTT GCT GA - #T TGC AAA GCA AAA CGT      528 Lys Ser Glu Trp Ala Thr His Lys Phe Ala As - #p Cys Lys Ala Lys Arg 165  - #               170  - #               175 - - GAC ACC CCC ACC TCA TGC ACT GTT GAT TAT TC - #T ACT GTG TAT TTT GTC 576 Asp Thr Pro Thr Ser Cys Thr Val Asp Tyr Se - #r Thr Val Tyr Phe Val 180      - #           185      - #           190 - - AAC ATT GAA GTC TGG GTA GAA GCA GAG AAT GC - #C CTT GGG AAG GTT ACA 624 Asn Ile Glu Val Trp Val Glu Ala Glu Asn Al - #a Leu Gly Lys Val Thr 195          - #       200          - #       205 - - TCA GAT CAT ATC AAT TTT GAT CCT GTA TAT AA - #A GTG AAG CCC AAT CCG 672 Ser Asp His Ile Asn Phe Asp Pro Val Tyr Ly - #s Val Lys Pro Asn Pro 210              - #   215              - #   220 - - CCA CAT AAT TTA TCA GTG ATC AAC TCA GAG GA - #A CTG TCT AGT ATC TTA 720 Pro His Asn Leu Ser Val Ile Asn Ser Glu Gl - #u Leu Ser Ser Ile Leu 225                 2 - #30                 2 - #35                 2 - #40 - - AAA TTG ACA TGG ACC AAC CCA AGT ATT AAG AG - #T GTT ATA ATA CTA AAA      768 Lys Leu Thr Trp Thr Asn Pro Ser Ile Lys Se - #r Val Ile Ile Leu Lys 245  - #               250  - #               255 - - TAT AAC ATT CAA TAT AGG ACC AAA GAT GCC TC - #A ACT TGG AGC CAG ATT 816 Tyr Asn Ile Gln Tyr Arg Thr Lys Asp Ala Se - #r Thr Trp Ser Gln Ile 260      - #           265      - #           270 - - CCT CCT GAA GAC ACA GCA TCC ACC CGA TCT TC - #A TTC ACT GTC CAA GAC 864 Pro Pro Glu Asp Thr Ala Ser Thr Arg Ser Se - #r Phe Thr Val Gln Asp 275          - #       280          - #       285 - - CTT AAA CCT TTT ACA GAA TAT GTG TTT AGG AT - #T CGC TGT ATG AAG GAA 912 Leu Lys Pro Phe Thr Glu Tyr Val Phe Arg Il - #e Arg Cys Met Lys Glu 290              - #   295              - #   300 - - GAT GGT AAG GGA TAC TGG AGT GAC TGG AGT GA - #A GAA GCA AGT GGG ATC 960 Asp Gly Lys Gly Tyr Trp Ser Asp Trp Ser Gl - #u Glu Ala Ser Gly Ile 305                 3 - #10                 3 - #15                 3 - #20 - - ACC TAT GAA GAT AGA CCA TCT AAA GCA CCA AG - #T TTC TGG TAT AAA ATA     1008 Thr Tyr Glu Asp Arg Pro Ser Lys Ala Pro Se - #r Phe Trp Tyr Lys Ile 325  - #               330  - #               335 - - GAT CCA TCC CAT ACT CAA GGC TAC AGA ACT GT - #A CAA CTC GTG TGG AAG 1056 Asp Pro Ser His Thr Gln Gly Tyr Arg Thr Va - #l Gln Leu Val Trp Lys 340      - #           345      - #           350 - - ACA TTG CCT CCT TTT GAA GCC AAT GGA AAA AT - #C TTG GAT TAT GAA GTG 1104 Thr Leu Pro Pro Phe Glu Ala Asn Gly Lys Il - #e Leu Asp Tyr Glu Val 355          - #       360          - #       365 - - ACT CTC ACA AGA TGG AAA TCA CAT TTA CAA AA - #T TAC ACA GTT AAT GCC 1152 Thr Leu Thr Arg Trp Lys Ser His Leu Gln As - #n Tyr Thr Val Asn Ala 370              - #   375              - #   380 - - ACA AAA CTG ACA GTA AAT CTC ACA AAT GAT CG - #C TAT CTA GCA ACC CTA 1200 Thr Lys Leu Thr Val Asn Leu Thr Asn Asp Ar - #g Tyr Leu Ala Thr Leu 385                 3 - #90                 3 - #95                 4 - #00 - - ACA GTA AGA AAT CTT GTT GGC AAA TCA GAT GC - #A GCT GTT TTA ACT ATC     1248 Thr Val Arg Asn Leu Val Gly Lys Ser Asp Al - #a Ala Val Leu Thr Ile 405  - #               410  - #               415 - - CCT GCC TGT GAC TTT CAA GCT ACT CAC CCT GT - #A ATG GAT CTT AAA GCA 1296 Pro Ala Cys Asp Phe Gln Ala Thr His Pro Va - #l Met Asp Leu Lys Ala 420      - #           425      - #           430 - - TTC CCC AAA GAT AAC ATG CTT TGG GTG GAA TG - #G ACT ACT CCA AGG GAA 1344 Phe Pro Lys Asp Asn Met Leu Trp Val Glu Tr - #p Thr Thr Pro Arg Glu 435          - #       440          - #       445 - - TCT GTA AAG AAA TAT ATA CTT GAG TGG TGT GT - #G TTA TCA GAT AAA GCA 1392 Ser Val Lys Lys Tyr Ile Leu Glu Trp Cys Va - #l Leu Ser Asp Lys Ala 450              - #   455              - #   460 - - CCC TGT ATC ACA GAC TGG CAA CAA GAA GAT GG - #T ACC GTG CAT CGC ACC 1440 Pro Cys Ile Thr Asp Trp Gln Gln Glu Asp Gl - #y Thr Val His Arg Thr 465                 4 - #70                 4 - #75                 4 - #80 - - TAT TTA AGA GGG AAC TTA GCA GAG AGC AAA TG - #C TAT TTG ATA ACA GTT     1488 Tyr Leu Arg Gly Asn Leu Ala Glu Ser Lys Cy - #s Tyr Leu Ile Thr Val 485  - #               490  - #               495 - - ACT CCA GTA TAT GCT GAT GGA CCA GGA AGC CC - #T GAA TCC ATA AAG GCA 1536 Thr Pro Val Tyr Ala Asp Gly Pro Gly Ser Pr - #o Glu Ser Ile Lys Ala 500      - #           505      - #           510 - - TAC CTT AAA CAA GCT CCA CCT TCC AAA GGA CC - #T ACT GGT CGG ACA AAA 1584 Tyr Leu Lys Gln Ala Pro Pro Ser Lys Gly Pr - #o Thr Gly Arg Thr Lys 515          - #       520          - #       525 - - AAA GTA GGG AAA AAC GAA GCT GTC TTA GAG TG - #G GAC CAA CTT CCT GTT 1632 Lys Val Gly Lys Asn Glu Ala Val Leu Glu Tr - #p Asp Gln Leu Pro Val 530              - #   535              - #   540 - - GAT GTT CAG AAT GGA TTT ATC AGA AAT TAT AC - #T ATA TTT TAT AGA ACC 1680 Asp Val Gln Asn Gly Phe Ile Arg Asn Tyr Th - #r Ile Phe Tyr Arg Thr 545                 5 - #50                 5 - #55                 5 - #60 - - ATC ATT GGA AAT GAA ACT GCT GTG AAT GTG GA - #T TCT TCC CAC ACA GAA     1728 Ile Ile Gly Asn Glu Thr Ala Val Asn Val As - #p Ser Ser His Thr Glu 565  - #               570  - #               575 - - TAT ACA TTG TCC TCT TTG ACT AGT GAC ACA TT - #G TAC ATG GTA CGA ATG 1776 Tyr Thr Leu Ser Ser Leu Thr Ser Asp Thr Le - #u Tyr Met Val Arg Met 580      - #           585      - #           590 - - GCA GCA TAC ACA GAT GAA GGT GGG AAG GAT GG - #T CCA GAA TTC ACT TTT 1824 Ala Ala Tyr Thr Asp Glu Gly Gly Lys Asp Gl - #y Pro Glu Phe Thr Phe 595          - #       600          - #       605 - - ACT ACC CCA AAG TTT GCT CAA GGA GAA ATT GA - #A GCC ATA GTC GTG CCT 1872 Thr Thr Pro Lys Phe Ala Gln Gly Glu Ile Gl - #u Ala Ile Val Val Pro 610              - #   615              - #   620 - - GTT TGC TTA GCA TTC CTA TTG ACA ACT CTT CT - #G GGA GTG CTG TTC TGC 1920 Val Cys Leu Ala Phe Leu Leu Thr Thr Leu Le - #u Gly Val Leu Phe Cys 625                 6 - #30                 6 - #35                 6 - #40 - - TTT AAT AAG CGA GAC CTA ATT AAA AAA CAC AT - #C TGG CCT AAT GTT CCA     1968 Phe Asn Lys Arg Asp Leu Ile Lys Lys His Il - #e Trp Pro Asn Val Pro 645  - #               650  - #               655 - - GAT CCT TCA AAG AGT CAT ATT GCC CAG TGG TC - #A CCT CAC ACT CCT CCA 2016 Asp Pro Ser Lys Ser His Ile Ala Gln Trp Se - #r Pro His Thr Pro Pro 660      - #           665      - #           670 - - AGG CAC AAT TTT AAT TCA AAA GAT CAA ATG TA - #T TCA GAT GGC AAT TTC 2064 Arg His Asn Phe Asn Ser Lys Asp Gln Met Ty - #r Ser Asp Gly Asn Phe 675          - #       680          - #       685 - - ACT GAT GTA AGT GTT GTG GAA ATA GAA GCA AA - #T GAC AAA AAG CCT TTT 2112 Thr Asp Val Ser Val Val Glu Ile Glu Ala As - #n Asp Lys Lys Pro Phe 690              - #   695              - #   700 - - CCA GAA GAT CTG AAA TCA TTG GAC CTG TTC AA - #A AAG GAA AAA ATT AAT 2160 Pro Glu Asp Leu Lys Ser Leu Asp Leu Phe Ly - #s Lys Glu Lys Ile Asn 705                 7 - #10                 7 - #15                 7 - #20 - - ACT GAA GGA CAC AGC AGT GGT ATT GGG GGG TC - #T TCA TGC ATG TCA TCT     2208 Thr Glu Gly His Ser Ser Gly Ile Gly Gly Se - #r Ser Cys Met Ser Ser 725  - #               730  - #               735 - - TCT AGG CCA AGC ATT TCT AGC AGT GAT GAA AA - #T GAA TCT TCA CAA AAC 2256 Ser Arg Pro Ser Ile Ser Ser Ser Asp Glu As - #n Glu Ser Ser Gln Asn 740      - #           745      - #           750 - - ACT TCG AGC ACT GTC CAG TAT TCT ACC GTG GT - #A CAC AGT GGC TAC AGA 2304 Thr Ser Ser Thr Val Gln Tyr Ser Thr Val Va - #l His Ser Gly Tyr Arg 755          - #       760          - #       765 - - CAC CAA GTT CCG TCA GTC CAA GTC TTC TCA AG - #A TCC GAG TCT ACC CAG 2352 His Gln Val Pro Ser Val Gln Val Phe Ser Ar - #g Ser Glu Ser Thr Gln 770              - #   775              - #   780 - - CCC TTG TTA GAT TCA GAG GAG CGG CCA GAA GA - #T CTA CAA TTA GTA GAT 2400 Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu As - #p Leu Gln Leu Val Asp 785                 7 - #90                 7 - #95                 8 - #00 - - CAT GTA GAT GGC GGT GAT GGT ATT TTG CCC AG - #G CAA CAG TAC TTC AAA     2448 His Val Asp Gly Gly Asp Gly Ile Leu Pro Ar - #g Gln Gln Tyr Phe Lys 805  - #               810  - #               815 - - CAG AAC TGC AGT CAG CAT GAA TCC AGT CCA GA - #T ATT TCA CAT TTT GAA 2496 Gln Asn Cys Ser Gln His Glu Ser Ser Pro As - #p Ile Ser His Phe Glu 820      - #           825      - #           830 - - AGG TCA AAG CAA GTT TCA TCA GTC AAT GAG GA - #A GAT TTT GTT AGA CTT 2544 Arg Ser Lys Gln Val Ser Ser Val Asn Glu Gl - #u Asp Phe Val Arg Leu 835          - #       840          - #       845 - - AAA CAG CAG ATT TCA GAT CAT ATT TCA CAA TC - #C TGT GGA TCT GGG CAA 2592 Lys Gln Gln Ile Ser Asp His Ile Ser Gln Se - #r Cys Gly Ser Gly Gln 850              - #   855              - #   860 - - ATG AAA ATG TTT CAG GAA GTT TCT GCA GCA GA - #T GCT TTT GGT CCA GGT 2640 Met Lys Met Phe Gln Glu Val Ser Ala Ala As - #p Ala Phe Gly Pro Gly 865                 8 - #70                 8 - #75                 8 - #80 - - ACT GAG GGA CAA GTA GAA AGA TTT GAA ACA GT - #T GGC ATG GAG GCT GCG     2688 Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Va - #l Gly Met Glu Ala Ala 885  - #               890  - #               895 - - ACT GAT GAA GGC ATG CCT AAA AGT TAC TTA CC - #A CAG ACT GTA CGG CAA 2736 Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pr - #o Gln Thr Val Arg Gln 900      - #           905      - #           910 - - GGC GGC TAC ATG CCT CAG         - #                  - # - #2754 Gly Gly Tyr Met Pro Gln 915 - -  - - (2) INFORMATION FOR SEQ ID NO:6: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 918 amino - #acids (B) TYPE: amino acid (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: protein - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: - - Met Leu Thr Leu Gln Thr Trp Val Val Gln Al - #a Leu Phe Ile Phe Leu 1               5 - #                 10 - #                 15 - - Thr Thr Glu Ser Thr Gly Glu Leu Leu Asp Pr - #o Cys Gly Tyr Ile Ser 20     - #             25     - #             30 - - Pro Glu Ser Pro Val Val Gln Leu His Ser As - #n Phe Thr Ala Val Cys 35         - #         40         - #         45 - - Val Leu Lys Glu Lys Cys Met Asp Tyr Phe Hi - #s Val Asn Ala Asn Tyr 50             - #     55             - #     60 - - Ile Val Trp Lys Thr Asn His Phe Thr Ile Pr - #o Lys Glu Gln Tyr Thr 65                 - # 70                 - # 75                 - # 80 - - Ile Ile Asn Arg Thr Ala Ser Ser Val Thr Ph - #e Thr Asp Ile Ala Ser 85 - #                 90 - #                 95 - - Leu Asn Ile Gln Leu Thr Cys Asn Ile Leu Th - #r Phe Gly Gln Leu Glu 100      - #           105      - #           110 - - Gln Asn Val Tyr Gly Ile Thr Ile Ile Ser Gl - #y Leu Pro Pro Glu Lys 115          - #       120          - #       125 - - Pro Lys Asn Leu Ser Cys Ile Val Asn Glu Gl - #y Lys Lys Met Arg Cys 130              - #   135              - #   140 - - Glu Trp Asp Gly Gly Arg Glu Thr His Leu Gl - #u Thr Asn Phe Thr Leu 145                 1 - #50                 1 - #55                 1 - #60 - - Lys Ser Glu Trp Ala Thr His Lys Phe Ala As - #p Cys Lys Ala Lys Arg 165  - #               170  - #               175 - - Asp Thr Pro Thr Ser Cys Thr Val Asp Tyr Se - #r Thr Val Tyr Phe Val 180      - #           185      - #           190 - - Asn Ile Glu Val Trp Val Glu Ala Glu Asn Al - #a Leu Gly Lys Val Thr 195          - #       200          - #       205 - - Ser Asp His Ile Asn Phe Asp Pro Val Tyr Ly - #s Val Lys Pro Asn Pro 210              - #   215              - #   220 - - Pro His Asn Leu Ser Val Ile Asn Ser Glu Gl - #u Leu Ser Ser Ile Leu 225                 2 - #30                 2 - #35                 2 - #40 - - Lys Leu Thr Trp Thr Asn Pro Ser Ile Lys Se - #r Val Ile Ile Leu Lys 245  - #               250  - #               255 - - Tyr Asn Ile Gln Tyr Arg Thr Lys Asp Ala Se - #r Thr Trp Ser Gln Ile 260      - #           265      - #           270 - - Pro Pro Glu Asp Thr Ala Ser Thr Arg Ser Se - #r Phe Thr Val Gln Asp 275          - #       280          - #       285 - - Leu Lys Pro Phe Thr Glu Tyr Val Phe Arg Il - #e Arg Cys Met Lys Glu 290              - #   295              - #   300 - - Asp Gly Lys Gly Tyr Trp Ser Asp Trp Ser Gl - #u Glu Ala Ser Gly Ile 305                 3 - #10                 3 - #15                 3 - #20 - - Thr Tyr Glu Asp Arg Pro Ser Lys Ala Pro Se - #r Phe Trp Tyr Lys Ile 325  - #               330  - #               335 - - Asp Pro Ser His Thr Gln Gly Tyr Arg Thr Va - #l Gln Leu Val Trp Lys 340      - #           345      - #           350 - - Thr Leu Pro Pro Phe Glu Ala Asn Gly Lys Il - #e Leu Asp Tyr Glu Val 355          - #       360          - #       365 - - Thr Leu Thr Arg Trp Lys Ser His Leu Gln As - #n Tyr Thr Val Asn Ala 370              - #   375              - #   380 - - Thr Lys Leu Thr Val Asn Leu Thr Asn Asp Ar - #g Tyr Leu Ala Thr Leu 385                 3 - #90                 3 - #95                 4 - #00 - - Thr Val Arg Asn Leu Val Gly Lys Ser Asp Al - #a Ala Val Leu Thr Ile 405  - #               410  - #               415 - - Pro Ala Cys Asp Phe Gln Ala Thr His Pro Va - #l Met Asp Leu Lys Ala 420      - #           425      - #           430 - - Phe Pro Lys Asp Asn Met Leu Trp Val Glu Tr - #p Thr Thr Pro Arg Glu 435          - #       440          - #       445 - - Ser Val Lys Lys Tyr Ile Leu Glu Trp Cys Va - #l Leu Ser Asp Lys Ala 450              - #   455              - #   460 - - Pro Cys Ile Thr Asp Trp Gln Gln Glu Asp Gl - #y Thr Val His Arg Thr 465                 4 - #70                 4 - #75                 4 - #80 - - Tyr Leu Arg Gly Asn Leu Ala Glu Ser Lys Cy - #s Tyr Leu Ile Thr Val 485  - #               490  - #               495 - - Thr Pro Val Tyr Ala Asp Gly Pro Gly Ser Pr - #o Glu Ser Ile Lys Ala 500      - #           505      - #           510 - - Tyr Leu Lys Gln Ala Pro Pro Ser Lys Gly Pr - #o Thr Gly Arg Thr Lys 515          - #       520          - #       525 - - Lys Val Gly Lys Asn Glu Ala Val Leu Glu Tr - #p Asp Gln Leu Pro Val 530              - #   535              - #   540 - - Asp Val Gln Asn Gly Phe Ile Arg Asn Tyr Th - #r Ile Phe Tyr Arg Thr 545                 5 - #50                 5 - #55                 5 - #60 - - Ile Ile Gly Asn Glu Thr Ala Val Asn Val As - #p Ser Ser His Thr Glu 565  - #               570  - #               575 - - Tyr Thr Leu Ser Ser Leu Thr Ser Asp Thr Le - #u Tyr Met Val Arg Met 580      - #           585      - #           590 - - Ala Ala Tyr Thr Asp Glu Gly Gly Lys Asp Gl - #y Pro Glu Phe Thr Phe 595          - #       600          - #       605 - - Thr Thr Pro Lys Phe Ala Gln Gly Glu Ile Gl - #u Ala Ile Val Val Pro 610              - #   615              - #   620 - - Val Cys Leu Ala Phe Leu Leu Thr Thr Leu Le - #u Gly Val Leu Phe Cys 625                 6 - #30                 6 - #35                 6 - #40 - - Phe Asn Lys Arg Asp Leu Ile Lys Lys His Il - #e Trp Pro Asn Val Pro 645  - #               650  - #               655 - - Asp Pro Ser Lys Ser His Ile Ala Gln Trp Se - #r Pro His Thr Pro Pro 660      - #           665      - #           670 - - Arg His Asn Phe Asn Ser Lys Asp Gln Met Ty - #r Ser Asp Gly Asn Phe 675          - #       680          - #       685 - - Thr Asp Val Ser Val Val Glu Ile Glu Ala As - #n Asp Lys Lys Pro Phe 690              - #   695              - #   700 - - Pro Glu Asp Leu Lys Ser Leu Asp Leu Phe Ly - #s Lys Glu Lys Ile Asn 705                 7 - #10                 7 - #15                 7 - #20 - - Thr Glu Gly His Ser Ser Gly Ile Gly Gly Se - #r Ser Cys Met Ser Ser 725  - #               730  - #               735 - - Ser Arg Pro Ser Ile Ser Ser Ser Asp Glu As - #n Glu Ser Ser Gln Asn 740      - #           745      - #           750 - - Thr Ser Ser Thr Val Gln Tyr Ser Thr Val Va - #l His Ser Gly Tyr Arg 755          - #       760          - #       765 - - His Gln Val Pro Ser Val Gln Val Phe Ser Ar - #g Ser Glu Ser Thr Gln 770              - #   775              - #   780 - - Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu As - #p Leu Gln Leu Val Asp 785                 7 - #90                 7 - #95                 8 - #00 - - His Val Asp Gly Gly Asp Gly Ile Leu Pro Ar - #g Gln Gln Tyr Phe Lys 805  - #               810  - #               815 - - Gln Asn Cys Ser Gln His Glu Ser Ser Pro As - #p Ile Ser His Phe Glu 820      - #           825      - #           830 - - Arg Ser Lys Gln Val Ser Ser Val Asn Glu Gl - #u Asp Phe Val Arg Leu 835          - #       840          - #       845 - - Lys Gln Gln Ile Ser Asp His Ile Ser Gln Se - #r Cys Gly Ser Gly Gln 850              - #   855              - #   860 - - Met Lys Met Phe Gln Glu Val Ser Ala Ala As - #p Ala Phe Gly Pro Gly 865                 8 - #70                 8 - #75                 8 - #80 - - Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Va - #l Gly Met Glu Ala Ala 885  - #               890  - #               895 - - Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pr - #o Gln Thr Val Arg Gln 900      - #           905      - #           910 - - Gly Gly Tyr Met Pro Gln 915 - -  - - (2) INFORMATION FOR SEQ ID NO:7: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: - - CCGCAGGTCG AGACAGC             - #                  - # - #   17 - -  - - (2) INFORMATION FOR SEQ ID NO:8: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: - - CAAACACCTT CTCGCGAA             - #                  - # - #  18 - -  - - (2) INFORMATION FOR SEQ ID NO:9: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: - - CTTCAGGGAG AGCGCGTGC             - #                  - # - # 19 - -  - - (2) INFORMATION FOR SEQ ID NO:10: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: - - TCATCAGGAC CCAGCTGTGC            - #                  - # - # 20 - -  - - (2) INFORMATION FOR SEQ ID NO:11: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: - - TTGACTAGTG ACACATTGTA C           - #                  - # - #21 - -  - - (2) INFORMATION FOR SEQ ID NO:12: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: - - TGAAACTTGC TTTGACCTTT            - #                  - # - # 20 - -  - - (2) INFORMATION FOR SEQ ID NO:13: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: - - GGTACGAATG GCAGCATACA            - #                  - # - # 20 - -  - - (2) INFORMATION FOR SEQ ID NO:14: - -      (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base - #pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear - -     (ii) MOLECULE TYPE: cDNA - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: - - CTGGACTGGA TTCATGCTGA            - #                  - # - # 20 __________________________________________________________________________

## Claims

1. An isolated and substantially purified DNA coding for a protein having the amino acid sequence of SEQ ID NO:4.

2. The DNA as claimed in claim 1 which hybridises under stringent conditions of between 35.degree. C. to 65.degree. C. and a salt concentration of about 0.9 M to the DNA sequence of SEQ ID NO:3.

3. A recombiant DNA comprising DNA as defined in claim 1 or claim 2.