# Patent

## Title
Intermediates for the manufacture of 4-acyloxy-3-hydroxyethylazetidinones

## Abstract

The present invention relates to new 1-.beta.-methyl-2-thiolic carbapenem derivatives represented by the following general formula (I) and producing method thereof. ##STR1## The above-mentioned compound is very stable against DHP-1 enzyme due to effective shielding of carbapenem ring structually and is highly effective in both gram-positive and gram-negative microorganism, especially has a remarkable effect on the Pseudomonas aeruginosa known as a disease germ having strong resistance.

## Background
1. Field of the invention

The present invention relates to new 1-.beta.-methyl-2-thiolic carbapenem derivatives represented by the following general formula (I) ##STR2##

In the formula (I), R.sup.1 denotes hydrogen or anion; when R.sup.1 is hydrogen, R.sup.2 denotes 2-hydroxyethylamino, 3-hydroxypropylamino, 2-(R)-hydroxypropylamino, 2-(S)-hydroxypropylamino, 2-(R)-hydroxybutylamino, 2-(S)-hydroxybutylamino, 4-hydroxybutylamino, 1-hydroxymethylpropyl-(R)-amino, 1-hydroxymethylpropyl-(S)-amino, 5-hydroxypentylamino, 1-isopropyl-2-hydroxyethyl-(R)-amino, 1-isopropyl-2-hydroxyethyl-(S)-amino, 6-hydroxyhexylamino, 1-(1-methylpropyl)-2-hydroxyethyl-(S)-amino, 1-(2-methylpropyl)-2-hydroxyethyl-(R)-amino, 1-(2-methylpropyl)-2-hydroxyethyl-(S)-amino, 2-(1,3-dihydroxypropyl)amino, 2,3-dihydroxypropylamino, N-(4-hydroxypiperidino), N-(2-hydroxymethylpiperidino), N-(2-hydroxyethylpiperidino), N-(3,3-dimethylpiperidino), N-(3-methylpiperidino), N-(1,2,5,6-tetrahydropyridinyl), N-(2-hydroxymethylpyrrolidino), N-homopiperidino, N-thiazoyl, N-thiomorpholinyl or N-(3-hydroxymethylthiazolyl); and when R.sup.1 is anion, R.sup.2 denotes N-(S-alkylthiomorpholinylium) of following general formula (1 or N-(S-alkylthiazolium) cation of following general formula). ##STR3##

(In the above formula 1 and 2, R.sup.3 denotes methyl or low alkyl group of C.sub.2 -C.sub.4)

2. Description of the prior art

The well-known compound among the carbapenem antibiotics which is a subject compound of the present invention is an antibiotic thienamycine which is produced by incubating special Streptomyces species and is active against both gram-positive and gram-negative bacteria. (Reference to the U.S. Pat. No. 3,950,357, Kahan et.al.). But, there was a defect such that this compound was decomposed by an enzyme named Dehydropeptidase-1 (DHP-1), so that the effect of this compound was decreased.

To solve the above problem, the method in which a inhibitor of DHP-1 enzyme named cilastatine is used together with a thienamycine was suggested by MERCK and CO., Inc. (Reference to the European Patent No. 48,301), but there was a still problem for using this composite of cilastatine and thienamycine.

Therefore, with concern the aforementioned problem, present inventors have studied for a long time to provide carbapenem antibiotics which are stable against DHP-1 enzyme and have synthesized new 1-.beta.-methyl-2-thiolic carbapenem derivatives(I) having .beta.-methyl group on the carbapenem ring. Also, present inventors have found that this new compound is very stable against the DHP-1 enzyme and is highly effective in both gram-positive and gram-negative microorganism, especially has a remarkable effect on the Pseudomonas aeruginosa known as a disease germ having strong resistance and that this effect arises from effective shielding of carbapenem ring by .beta.-methyl group in 1-.beta.-methyl-carbapenem derivatives(I).

In the present invention, the carbapenem ring in 1-.beta.-methyl-2-thiolic carbapenem derivatives having general formula (I) has 4 chiral carbon atoms and the maximum number of optical isomer configuration that can exist is 16(=2.sup.4). Among these optical isomer configurations, especially (1R, 5S, 6S,8R)-configuration represented by following formula is superior to in vivo activity. ##STR4##

Also, in the 1-.beta.-methyl-2-thiolic carbapenem derivatives (I) according to the present invention, thiol derivatives substituted on C-2 position of carbapenem ring show the optical isomerism according to circumstance and the maximum number of optical isomer configuration that can exist is 4(=2.sup.2). Among these optical isomer configurations, especially (3S, 5S)-configuration represented by following formula shows remarkable effect in vivo activity. ##STR5##

1-.beta.-methyl-2-thiolic carbapenem derivatives (I) according to the present invention can be prepared by, for example, following reaction procedure: (II) as a starting material with diphenyl chlorophosphate or trifluoromethanesulfonic anhydride in the present of a base, ##STR6## (In the above formula (II) and (III), p.sup.1 denotes a --COOH protecting group and X denotes --OPO(OPh).sub.2 or --OSOOCF.sub.3) formula (V) by reaction of the above compound (III) with thiol derivatives having following general formula (IV) in the present of a base, and ##STR7## (In the above formula (IV) and (V), R.sup.2 is the same as defined in formula (I) and p.sup.2 denotes an amine protecting group) derivatives (I) by adding an organic solvent, phosphate or 4-MOPS buffer solution and palladium-on-charcoal catalyst into said protected carbapenem (V) in sequence and eliminating the protecting group by injecting with hydrogen. ##STR8##

In the process of the present invention, the group p.sup.1 is a protecting group which is generally used for protecting --COOH and, for example, is p-nitrobenzyl or allyl group. The group P.sup.2 is a protecting group which is used for protecting amine and, for example, is p-nitrobenzyloxycarbonyl group.

Also, in 1-.beta.-methyl-2-thiolic carbapenem derivatives (I) according to the present invention, the compound in which R.sup.1 group is anion can be produced by forming a cation on R.sup.2 position of protected carbapenem(V) obtained from step (2) by alkylation reaction, which followed by step (3) in which said protecting group is eliminated. For example, zwitter-ionic 1-.beta.-methyl-2-thiolic carbapenem derivatives (I) in which R.sup.2 is N-(S-alkylthiomopholinylium) can be produced by following reaction mechanism. ##STR9## (in this formula, R.sup.3 denotes methyl or low alkyl group of C.sub.2 -C.sub.4)

Zwitter-ionic 1-.beta.-methyl-2-thiolic carbapenem derivatives(I) synthesized by above-mentioned method Is generally quite soluble in water. R.sup.2 which is suitable for forming a cation by alkylation reaction is N-thiazolyl or N-thiomorpholinyl group. In the above-mentioned alkylation reaction, conventional alkylating agents can be used and for example, methyl trifluoromethanesulfonate and alkyl halide such as methyl iodide are preferable.

Hereinafter, the present invention will be further described by referring to the following example. 5S,6S)-2-[(3S,5S)-5-(thiomorpholinyl-N-carbonyl)-1-(p-nitrobenzyloxycarbon yl)pyrrolidine-3-ylthio]-6-[(R)-1-hydroxyethyl]-1-methylcarbapene,-3-carbox ylate

(4R,5R,6S,8R)-p-nitrobenzyl-4-methyl-6-(1-hydroxyethyl)-1-azabicyclo-(3.2.0 )heptane-3,7-dione-2-carboxylate(2.0 g, 0.0055 mol) solution in acetonitrile was cooled down to 0.degree. C. under the nitrogen atmosphere and was treated with diphenyl chlorophosphate(0.96 ml, 0.006 mol) in N,N-diisopropylethylamtne(0.82 ml, 0.006 mol). Thus-obtained mixture was agitated for 20 min. at 5.degree. C. and cooled down to -20.degree. C. and then added N,N-diisopropylethylamine (0.82 ml, 0.006 mol) and thiol compound (2.26 g, 0.0055 mol). This reaction mixture was agitated for 1 hour at -20.degree. C. and further agitated for 30 min. at 0.degree. C. 50 ml of ethylacetate was added to the reaction mixture and organic layer thereof was washed with 10% NaHCO.sub.3 and saline solution and dried using anhydrous MgSO.sub.4. The target material was obtained by removing an organic solvent and separating through a silica gel column. .sup.1 H-NMR(CDCl.sub.3): .delta.1.15(d, 3H, .beta.-CH.sub.3), 1.25(d, 3H, CH.sub.3 CHOH), 2.10, 2.80(m, 1H, pyrr.H), 2.84-295 (bs, 4H), 3.35(dd, 1H, C.sub.6 -H), 3.40-3.53(bs, 5H), 3.55(m, 1H, pyrr.H), 4.15(bs, 2H, pyrr.H), 4.18(dd, 1H, C.sub.5 -H), 4.25(quintet, 1H, CH.sub.3 CHOH), 4.75(m, 1H, pyrr.H), 5.25-5.40(quintet, 2H), 7.53(d, 4H), 7.65(d, 2H), 8.15(d, 2H) (1R,5S,6S)-6-[(R)-1-hydroxymethyl]-2[(3S,5S)-5-((S-methylthiomorpholinyl)a mino-N-carbonyl)pyrrolidine-3-ylthio]-1-methyl carbapenem-3-carboxylic acid

p-nitrobenzyl-(1R,5S,6S)-2-[(3S,5S)-5-(thiomorpholinyl-N-carbonyl)-1-(p-nit robenzyloxycarbonyl)pyrrolidine-3-ylthio]-6-[(R)-1-hydroxyethyl]-1-methylca rbapenem-3-carboxylate(1.0 g, 0.0013 mol) solution in anhydrous dichloromethane(20 ml) was treated with methyl trifluoro methanesulfonate(0.40 ml, 0.0013 mol). The foamy solid compound was obtained by agitating for 2 hours at room temperature and removing the solvent. In this solid compound, tetrahydrofuran(20 ml), saline buffer solution(pH=7)(20 ml) and palladium-on-charcoal catalyst 1.0 g were added in sequence. Thus-obtained mixture was put into the hydrogen injector and shaken well for 1 hour. The mixture was filtered with a celite and filtrate was washed twice with ethylether. The target material was obtained by freeze-drying the water layer and separating by column of Diaion HP-20(trade name of an ion-exchange resin manufactured by Mitsubishi Chemical Industries Limited.).

.sup.1 H-NMR(D.sub.2 O): .delta.1.22(d, 3H, .beta.-CH.sub.3), 1.26(d, 3H, CH.sub.3 CHOH), 2.10, 3.05(m, 1H, pyrr.H), 3.08-3.11(bs, 4H), 3.05(s, 3H), 3.30(dq, 1H, C.sub.1 -H) 3.35-3.55(bs, 5H), 3.95-4.10(bs, 2H, pyrr.H), 4.25-4.30(m, 2H), 4.45(m, 1H, pyrr.H) ##STR10## (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2[(3S,5S)-5-((2-hydroxyethyl)aminocarbon yl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3carboxylic acid

(4R,5R,6S,8R) p-nitrobenzyl-4-methyl-6-(1-hydroxyethyl)-1-azabicyclo-(3.2.0)heptane-3,7- dione-2-carboxylate(2.0 g, 0.0055 mol) solution in acetonitrile (20 ml) was cooled down to 0.degree. C. under nitrogen atmosphere and treated with N,N-diisopropylethylamine(0.82 ml, 0.006 mol) and diphenyl chlorophosphate(0.96 ml, 0.006 mol). Thus-obtained mixture was agitated for 30 min at 5.degree. C. and cooled down to -20.degree. C. and then added N,N-diisopropylethylamine (0.82 ml, 0.006 mol) and (2S,4S)-2-(2-hydroxyethyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxycarbo nylpyrrolidine(2.26 g, 0.0055 mol). The reaction mixture was agitated for 1 hour at -20 .degree. C. and further agitated 30 min. at 0.degree. C. The reaction mixture was added 50 ml of ethyl acetate and washed organic layer with 10% NaHCO.sub.3 and saline solution and dried using anhydrous MgSO.sub.4. The material was obtained by removing an organic layer from the mixture and separating through the silica gel column. In this solid compound, tetrahydrofuran(20 ml), saline buffer solution(pH=7, 20 ml) and palladium-on-charcoal catalyst 1.0 g were added in sequence, and this mixture was put into hydrogen injector and shaken well for 1 hour. The reaction mixture was filtered using a celite and washed twice with ethyl ether (20 ml). The target material was obtained by freeze-drying the water layer and separating by column of Diaion HP-20.

.sup.1 H-NMR(D.sub.2 O): .delta.1.19(d, 3H, .beta.-CH.sub.3), 1.28(d, 3H, CH.sub.3 CHOH), 2.02, 2.83(m, 1H, pyrr.H), 3.30-3.48(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.63-3.70(m, 3H, pyrr.H and CH.sub.2 OH), 3.97(m, 1H, pyrr.H), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.35(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((3-hydroxypropyl)aminocarb onyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3 -carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(3-hydroxypropyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxycarb onyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.19(d, 3H, .beta.-CH.sub.3), 1.22(d, 3H, CH.sub.3 CHOH), 2.05, 2.87(m, 1H, pyrr.H), 3.29-3.46(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.61-3.70(m, 3H, pyrr.H and CH.sub.2 OH), 3.97(m, 1H, pyrr.H), 4.20-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.35(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((2-(R)-hydroxypropyl)amino carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The Procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-(R)-hydroxypropyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxy carbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.15-1.21(dd, 6H, CH.sub.3 and .beta.-CH.sub.3), 1.26(d, 3H, CH.sub.3 CHOH), 2.05, 2.93(m, 1H, pyrr.H), 3.20-3.48(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.87(m, 1H, pyrr.H), 3.91-4.08(m, 2H, pyrr.H and CHOH), 4.18-4.28(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.50(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hxdroxyethyl]-2-[(3S,5S)-5-((2-(S)-hydroxypropyl)amino carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-(S)-hydroxypropyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxy carbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.17-1.23 (dd, 6H, CH.sub.3 and .beta.-CH.sub.3), 1.27(d, 3H, CH.sub.3 CHOH), 2.05, 2.89(m, 1H, pyrr.H), 3.20-3.51(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.77(m, 1H, pyrr, H), 3.91-4.10(m, 2H, pyrr.H and CHOH), 4.18-4.28(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.50(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((2-(R)-hydroxybutyl)aminoc arbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-[2-(R)-hydroxybutyl]aminocarbonyl-4-mercapto-1-p-nitrobenzyloxyc arbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.15 (t, 3H, -CH.sub.3), 1.19(d, 3H, .beta.-CH.sub.3), 1.28(d, 3H, CH.sub.3 CHOH), 2.05, 2.90(m, 1H, pyrr.H), 3.37-3.48(m, 3H, CH.sub.2 CH.sub.3 and C.sub.1 -H), 3.53-3.62(m, 2H, pyrr.H and C.sub.6 -H), 3.75(m, 1H, pyrr.H), 3.94-4.08(m, 2H, pyrr.H and CHOH), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.35(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((2-(S)-hydroxybutyl)aminoc arbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-(S)-hydroxybutyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxyc arbonyl pyrrolidine.

.sup.1 H-NHR(D.sub.2 O ): .delta.1.15 (t, 3H, -CH.sub.3), 1.19(d, 3H, .beta.-CH.sub.3), 1.29(d, 3H, CH.sub.3 CHOH), 2.05, 2.93(m, 1H, pyrr.H), 3.38-3.54(m, 3H, CH.sub.2 CH.sub.3 and C.sub.1 -H), 3.57-3.68(m, 2H, pyrr.H and C.sub.6 -H), 3.75(m, 1H, pyrr.H), 3.94-4.10(m, 2H, pyrr.H and CHOH), 4.22-4.35(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.45(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((4-hydroxybutyl)aminocarbo nyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(4-hydroxybutyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxycarbo nyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.19(d, 3H, .beta.-CH.sub.3), 1.24(d, 3H, CH.sub.3 CHOH), 1.51-1.62(bs, 4H, CH.sub.2 CH.sub.2 C.sub.2 OH), 2.05, 2.93(m, 1H, pyrr.H), 3.18-3.50(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.55(bs, 2H, CH.sub.2 CH.sub.2 OH), 3.77(m, 1H, pyrr.H), 4.05(m, 1H, pyrr.H), 4.25-4.31 (m, 2H, C.sub.6 -H and CH.sub.3 CHOH), 4.45(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((1-hydroxymethyl)propyl-(R )-aminocarbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-hydroxymethyl)propyl-(R)-aminocarbonyl-4-mercapto-1-p-nitrobe nzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.90-0.99 (t, 3H, CH.sub.3), 1.19(d, 3H, .beta.-CH.sub.3), 1.29(d, 3H, CH.sub.3 CHOH), 1.90(m, 1H, CH), 2.08, 2.99(m, 1H, pyrr.H), 3.30-3.68(m, 4H, CONHCH, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.73-3.95(m, 4H, pyrr.H and CH.sub.2 OH), 4.08(m, 1H, pyrr.H), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.55(t, 1H, pyrr.H) -5-((1-hydroxymethyl)propyl-(S)-aminocarbonyl)pyrrolidine-3-ylthio]-1-meth ylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-hydroxymethyl)propyl-(S)-aminocarbonyl-4-mercapto-1-p-nitrobe nzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.90-0.98 (t, 3H, CH.sub.3), 1.19(d, 3H, .beta.-CH.sub.3), 1.29(d, 3H, CH.sub.3 CHOH), 1.90(m, 1H, CH), 2.08, 2.99(m, 1H, pyrr.H), 3.30-3.68(m, 4H, CONHCH, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.73-3.95(m, 4H, pyrr.H and CH.sub.2 OH), 4.08(m, 1H, pyrr.H), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.55(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((5-hydroxypentyl)aminocarb onyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(5-hydroxypentyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxycarb onyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.19(d, 3H, .beta.-CH.sub.3), 1.25(d, 3H, CH.sub.3 CHOH), 1.52-1.73(m, 6H, 3CH.sub.2), 2.02, 2.89(m, 1H, pyrr.H), 3.21-3.48(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.58(bs, 2H, CH.sub.2 CH.sub.2 OH), 3.80(m, 1H, pyrr.H), 4.08(m, 1H, pyrr.H), 4.25(m, 2H, CH.sub.5 -H and CH.sub.3 CHOH), 4.45(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxethyl]-2-[(3S,5S)-5-((1-isopropyl-2-hydroxyethyl )-(R)-aminocarbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-isopropyl-2-hydroxyethyl)-(R)-aminocarbonyl)-4-mercapto-1-p-n itrobenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.90-0.98(m, 6H, 2CH.sub.3), 1.19(d, 3H, .beta.-CH.sub.3), 1.29(d, 3H, CH.sub.3 CHOH), 1.90(m, 1H, CH) 2.08, 2.99(m, 1H, pyrr.H), 3.30-3.68(m, 4H, CONHCH, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.73-3.95(m, 4H, pyrr.H and CH.sub.2 OH), 4.08(m, 1H, pyrr.H), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.55(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(1-isopropyl-2-hydroxyethyl )-(S)-aminocarbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-isopropyl-2-hydroxyethyl)-(S)-aminocarbonyl)-4-mercapto-1-p-n itrobenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.09-0.98(m, 6H, 2Ch.sub.3), 1.19(d, 3H, .beta.-CH.sub.3), 1.29(d, 3H, CH.sub.3 CHOH, 1.90(m, 1H, CH), 2.08, 2.99(m, 1H, pyrr.H), 3.30-3.68(m, 4H, CONHCH, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.73-3.95(m, 4H, pyrr.H and CH.sub.2 OH), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.55(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroethyl]-2-[3S,5S)-5-((6-hydroxyhexyl)aminocarbonyl )pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid.

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(6-hydroxypropyl)aminocarbonyl-4-mercapto-1-p-nitrobenzyloxycarb onyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.18(d, 3H, .beta.-CH.sub.3), 1.2(d, 3H, CH.sub.3 CHOH), 1.52-1.80(m, 8H, 4CH.sub.2), 2.05, 2.90(m, 1H, pyrr.H), 3.20-3.51(m, 5H, CONHCH.sub.2, pyrr.H, C.sub.6 -H and C.sub.1 -H), 3.55(bs, 2H, CH.sub.2 CH.sub.2 OH) 3.81(m, 1H, pyrr.H), 4.05(m, 1H, pyrr.H), 4.25-4.31 (m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.45(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-((1-isobutyl-2-hydroxyethyl -(R)-aminocarbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-isobutyl-2-hydroxyethyl)aminocabonyl-4-mercapto-1-p-nitrobenz yloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.85-0.96(m, 6H, 2CH.sub.3), 1.19-1.63(bs, 9H), 2.02, 2.89(m, 1H, pyrr.H), 3.35-3.50(m, 2H), 3.56-3.67(dd, 1H, C.sub.6 -H), 3.70-3.85(m, 1H, pyrr.H), 3.95-4.10(bs, 2H, CHCH.sub.2 OH), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.60(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(1-(2-methylpropyl)-2-hydro xyethyl)-(S)-aminocarbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carbo xylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-isobutyl-2-hydroxyethyl-(R)-aminocarbonyl)-4-mercapto-1-p-nit robenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.85-0.96(m, 6H, 2CH.sub.3), 1.19-1.63(bs, 9H), 2.02, 2.89(m, 1H, pyrr.H), 3.35-3.50(m, 2H), 3.56-3.67(dd, 1H, C.sub.6 -H), 3.70-3.85(m, 1H, pyrr.H), 3.95-4.10(bs, 2H, CHCH.sub.2 OH), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH) 4.62(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(1-(2-methylpropyl)-2-hydro xyethyl)-(S)-aminocarbonyl)pyrrolidine-3-yl-thio]-1-methylcarbapenem-3-carb oxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1-isobutyl-2-hydroxyethyl-(S)-aminocarbonyl)-4-mercapto-1-p-nit robenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.85-0.96(m, 6H, 2CH.sub.3), 1.19-1.63(bs, 9H), 2.20, 2.89(m, 1H, pyrr.H), 3.53-3.50(m, 2H), 3.56-3.67(dd, 1H, C.sub.6 -H), 3.70-3.85(m, 1H, pyrr.H), 3.95-4.10(bs, 2H, CHCH.sub.2 OH), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.62(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-(1,3-dihydroxypropyl)-am inocarbonyl)pyrrolidine-3-ylthio]-1-methylcabapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(di-(2-hydroxyethyl)aminocarbonyl)-4-mercapto-1-p-nitrobenzyloxy carbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.22(d, 3H, .beta.-CH.sub.3), 1.33(d, 3H, CH.sub.3 CHOH), 2.10, 2.99(m, 1H, pyrr.H), 3.35--3.58(m, 2H, C.sub.6 -H and C.sub.1 -H), 3.63-3.88(m, 6H, pyrr.H and CH.sub.2 OH), 4.09(m, 1H, pyrr.H), 4.25-4.31(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.55(t, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(2,3-dihydroxypropyl)aminoc arbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(di-(2,3-di-(hydroxy)propyl aminocarbonyl)-4-mercapto-1-p-nitrobenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.22(d, 3H, .beta.-CH.sub.3), 1.30(d, 3H, CH.sub.3 CHOH), 1.40-1.71(bs, 2H, piperidine H), 1.90-2.10(bs, 3H, piperdine H and pyrrolidine C.sub.4 H), 3.15(m, 1H, pyrrolidine C.sub.4 -H), 3.30(dq, 1H, C.sub.1 -H), 3.35-3.55(bs, 5H, piperidine H and C.sub.6 -H) 3.95-4.15(bs, 2H, pyrrolidine C.sub.2 -H), 4.25-4.30(m, 2H, C.sub.5 -H and CH.sub.3 CHOH), 4.85(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(4-hydroxypiperidinyl-N-car bonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(4-hydroxy piperidinyl-N-carbonyl)-4-mercapto-1-p-nitrobenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.18(d, 3H, .beta.-CH.sub.3), 1.20(d, 3H, CH.sub.3 CHOH), 1.50-1.85(bs, 5H, piperidine H), 3.40(dd, 1H, C.sub.6 -H), 3.45-3.90(bs, 3H, piperidine H), 4.25(dd, 1H, C.sub.5 -H), 4.85(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-hydroxymethylpiperidinyl -N-carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-hydroxymethylpiperidinyl-N-carbonyl)-4-mercapto-1-p-nitrobenz yloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.18(d, 3H, .beta.-CH.sub.3), 1.20(d, 3H, CH.sub.3 CHOH), 1.50-1.85(bs, 6H, piperdine H), 3.05(d, 2H, CH.sub.2 OH), 3.40(dd, 1H, C.sub.6 -H), 3.45-3.90(bs, 3H, piperidine H), 4.25(dd, 1H, C.sub.5 -H), 4.85(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-hydroxyethylpiperidinyl- N-carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-hydroxyethylpiperidinyl-N-carbonyl)-4-mercapto-1-p-nitrobenzy loxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.19(d, 3H, .beta.-CH.sub.3), 1.22(d, 3H, CH.sub.3 CHOH), 1.50-1.85(bs, 8H, piperidine H and CH.sub.2 CH.sub.2 OH), 3.10(t, 2H, CH.sub.2 OH), 3.35(dq, 1H, C.sub.1 -H), 4.80(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(3,3-dimethylpiperidinyl-N- carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(3-dimethylpiperdinyl-N-carbonyl)-4-mercapto-1-p-nitrobenzyloxyc arbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.90(d, 6H, 2CH.sub.3), 1.20(d, 3H, .beta.-CH.sub.3), 1.25(d, 3H, CH.sub.3 CHOH), 1.35-1.65(bs, 5H, piperidine H), 2.85(m, 1H, pyrrolidine C.sub.4 -H), 3.33(dq, 1H, C.sub.1 -H), 3.45(dd, 1H, C.sub.6 -H), 4.20(dq, 1H, CH.sub.3 CHOH), 4.25(dd, 1H, C.sub.5 -H), 4.82(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(3-methylpiperidinyl-N-carb onyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(3-methylpiperidinyl-N-carbonyl)-4-mercapto-1-p-nitrobenzyloxyca rbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.0.90(d, 3H, --CH.sub.3), 1.20(d, 3H, .beta.-CH.sub.3), 1.28(d, 3H CH.sub.3 CHOH), 1.50-1.85(bs, 5H, piperdine H), 3.15(m, 1H, pyrrolidine C.sub.4 -H), 3.35(dq, 1H, C.sub.1 -H), 3.50(dd, 1H, C.sub.6 -H), 3.95-4.15(bs, 4H, piperidine H), 4.25(dd, 1H, C.sub.5 -H), 4.85(m, 1H, pyrrolidine C.sub.5 -H) 5S)-5-(1,2,5,6-tetrahydropyridinyl-N-carbonyl)pyrrolidine-3-ylthio]-1-meth ylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(1,2,5,6-tetrahydropyridinyl-N-carbonyl)-4-mercapto-1-p-nitroben zyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.25(d, 3H, .beta.-CH.sub.3), 1.35(d, 3H, CH.sub.3 CHOH), 1.60-1.78(bs, 2H, piperidine H), 3.12(m, 1H, pyrrolidine C.sub.4 -H), 3.40(dq, 1H, C.sub.1 -H), 3.55(dd, 1H, C.sub.6 -H), 4.28(dd, 1H, C.sub.6 -H), 4.85(m, 1H, pyrrolidine C.sub.5 -H), 5.80-5.95(t, piperidine CH.dbd.CH) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-hydroxymethylpyrrolidiny l-N-carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-hydroxymethylpyrrolidinyl-N-carbonyl)-4-mercapto-1-p-nitroben zyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.25(d, 3H, .beta.-CH.sub.3), 1.33(d, 3H, CH.sub.3 CHOH), 1.85-2.15(bs, 4H, piperidine H), 3.10(m, 3H, pyrrolidine C.sub.4 -H and CH.sub.2 OH), 3.40(dq, 1H, C.sub.1 -H), 3.50(dd, 1H, C.sub.6 -H), 4.28(dd, 1H, C.sub.6 -H), 4.88(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(homopiperidinyl-N-carbonyl )pyrroline-3-ylthio]-1-methylcarbanenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(homopiperidinyl-N-carbonyl)-4-mercapto-1-p-nitrobenzyloxycarbon yl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.20(d, 3H, .beta.-CH.sub.3), 1.25(d, 3H, CH.sub.3 CHOH), 1.55-1.80(bs, 8H, homopiperidine H), 3.10(m, 1H, pyrrolidine C.sub.4 -H), 3.34(dq, 1H, C.sub.1 -H), 3.55(dd, 1H, C.sub.6 -H), 4.28(dd, 1H, C.sub.5 -H), 4.85(m, 1H, pyrrolidine C.sub.5 -H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(thiazolyl-N-carbonyl)pyrro lidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(thiazolyl)carbonyl-4-mercapto-1-p-nitrobenzyloxycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.23(d, 3H, .beta.-CH.sub.3), 1.30(d, 3H, CH.sub.3 CHOH), 2.08, 3.55(m, 1H, pyrr.H), 3.33(dq, 1H, C.sub.1 -H), 3.38-3.55(bs, 5H), 3.98-4.10(bs, 2H, pyrr.H), 4.25-4.31(m, 2H), 4.45(m, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-2-[(3S,5S)-5-(2-hydroxymethylthiazolyl-N -carbonyl)pyrrolidine-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 3 and the used thiol compound was (2S,4S)-2-(2-hydroxymethylthiazolyl-N-carbonyl-4-mercapto-1-p-nitrobenzylo xycarbonyl pyrrolidine.

.sup.1 H-NMR(D.sub.2 O): .delta.1.23(d, 3H, .beta.-CH.sub.3), 1.30(d, 3H, CH.sub.3 CHOH), 2.08, 3.55(m, 1H, pyrr.H), 3.10-3.21(bs, 2H), 3.33(dq, 1H, C.sub.1 -H), 3.38-3.55(bs, 5H), 3.98-4.10(bs, 2H, pyrr.H), 4.25-4.31(m, 2H), 4.45(m, 1H, pyrr.H) (1R,5S,6S)-6-[(R)-1-hxdroxyethyl]-2-[(3S,5S)-5-((S-methyl-thiazolyl-N-carb onylpyrrolidine)-3-ylthio]-1-methylcarbapenem-3-carboxylic acid

The procedure was carried out according to the Example 2 using the thiol compound of Example 30.

.sup.1 H-NMR(D.sub.2 O): .delta.1.23(d, 3H, .beta.-CH.sub.3), 1.30(d, 3H, CH.sub.3 CHOH), 2.08, 3.55(m, 1H, pyrr.H), 3.10(s, 3H), 3.33(dq, 1H, C.sub.1 -H), 3.38-3.55(bs, 5H), 3.98-4.10(bs, 2H, pyrr.H), 4.25-4.31(m, 2H), 4.45(m, 1H, pyrr.H)

## Claims

1. 1-.beta.-methyl-2-thiolic carbapenem derivatives having the following formula (I): ##STR11## in the formula (I), R.sup.1 denotes anion and R.sup.2 denotes N-(S-alkylthiomorpholinylium) of following formula 1 or N-(S-alkylthiazolium) cation of following formula 2, ##STR12## (In the above formula 1 and 2, R.sup.3 denotes methyl or low alkyl group of C.sub.2 -C.sub.4).

2. 1-.beta.-methyl-2-thiolic carbapenem derivatives (I) according to claim 1, wherein optical isomer configuration of said carbapenem ring is (1R,5S,6S,8R).

3. 1-.beta.-methyl-2-thiolic carbapenem derivatives (I) according to claim 1, wherein optical isomer configuration of thiol derivatives substituted on C-2 position of carbapenem ring is (3S,5S).