Nanosys

Worker Activity EPA SanitizedTask Worker Activity Personal Protective Equipment/Eng

Controls Phy Forms and % product # Workers

Exposed Max

(hrs/day) Duration

(days/yr)

LH

‐ ST

‐ 1 Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 0% 3 11.00 255

LH

‐ ST

‐ 2 Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 0% 3 11.00 255

GEN II

ST

‐ 1 Closed tank system process using EZI

‐

DOCK loading bags (<1ppb I air) and

TOP liquid transfer system: nitrile

gloves, safety glasses, lab coat 0% 1 0.50 255

GEN II

ST

‐ 2 Closed tank system process using EZI

‐

DOCK loading bags (<1ppb I air) and

TOP liquid transfer system: nitrile

gloves, safety glasses, lab coat 0% 1 0.50 255

LS

‐ C1 Add 400

‐ 613 g trioctylphosphine oxide (TOPO), 79

‐ 138g

tetradecylphosphonic acid (TDPA), and stir bar to a 3 L 5

‐

neck RB Flask. Evacuate and refill with Nitrogen 3 times.

Turn on nitrogen purge, Add 184

‐ 287 ml of Cd Stock

(Cd/TOP) using 24 ml syringes to flask. Cool to 110°C.

Evacuate the flask. Hold at 110 °C under vacuum for 30

min

‐ 3 hours. Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat

0% 3 0.25 255

LS

‐ C2 Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 0% 3 0.25 255

LS

‐ C3 Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 3 0.25 255

LS

‐ C4 Glove box, fume hood, nitrile gloves,

safety glasses, lab coat 3 0.50 255

LS

‐ C5 Remove thermocouple. Replace septa cap and addition

funnels with secured glass stoppers. Bring flask into the

glove

‐ box under vacuum. Fume hood, nitrile gloves

, safety

glasses, lab coat 3 0.25 255

LS

‐ C6 Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 3 0.90 255

LS

‐ C7 Pour off supernatant. Dissolve QD with TDPA Ligands

with Hexane 2

‐ 4 ml per bottle syringes out and repeated

once. Collect on syringed material in one bottle. Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 3 0.80 255

LS

‐ C8 Prepare 1 mL sample for QAQC testing. Determine

absorbance λ using the UV/vis spectrometer and

emission λ and distribution full width half max using the

fluorometer. Sample is prepared in glove box, taken out

in a closed cuvette to do a UV

‐ Vis reading. In fume

hood, a sample is made using wet hexane to prepare the

second reading, using the fluorometer. Fume hood, nitrile gloves

, safety

glasses, lab coat

3 0.60 255

LS

‐ C9 Laboratory glassware is placed into a 30% Potassium

Hydroxide (KOH) / 70% Isopropyl Alcohol (IPA) base

bath for a minimum of thirty minutes. Splash protection including gloves

(PVC/Butyl/Neoprene outer gloves in

combination with inner nitrile gloves),

safety glasses, splash shield, lab coat

and splash apron. 0.01% of residual QD

(liquid) 3 0.50 255

LS

‐ C10 Retrieve glassware and pour off any residual base bath

and gently submerge into sodium bicarbonate rinse bath

to neutralize the 30% Potassium Hydroxide (KOH) / 70%

Isopropyl Alcohol (IPA). Splash protection including gloves

(PVC/Butyl/Neoprene outer gloves in

combination with inner nitrile gloves),

safety glasses, splash shield, lab coat

and splash apron. 0.01% of residual QD

(liquid) 3 0.10 255 Stock Solution Preparation Procedure

Lab Scale Core Wash Procedure Gen II Reactor Stock Solution Preparation Procedure

End of Document

Lab Scale Core Reaction Procedure

Lab Scale Glassware Washing

PMN Page 9 Attachment

Section A--(2) Occupational Exposure

Page 1 of 3Nanosys

Worker Activity EPA SanitizedTask Worker Activity Personal Protective Equipment/Eng

Controls Phy Forms and % product # Workers

Exposed Max

(hrs/day) Duration

(days/yr)

LS

‐ C11 Retrieve glassware from neutralizer bath and place into

nitric acid bath bin. Splash protection including gloves

(PVC/Butyl/Neoprene outer gloves in

combination with inner nitrile gloves),

safety glasses, splash shield, lab coat

and splash apron. 0% 3 0.10 255

LS

‐ C12 Place glassware in bin and transport to rinse sink.

Glassware is washed minimal of two times with tap

water, inspected and then rinsed with deionized water. Nitrile gloves, safety glasses, and lab

coat. 0% 3 0.10 255

LS

‐ C13 Glassware is placed on pegs to dry. If the wet glasswear

is needed hastily, it can be rinsed with ethanol and then

drip dried before placing in a designated glass drying

oven (100° C). Glassware is dried for a minimum of

fifteen minutes before being removed. Thermal gloves, nitrile gloves, safety

glasses, and lab coat.

0% 3 0.20 255

LS

‐ C14 The hot glass should be cooled in a designated area with

visible warning signs for hot glassware. Once cooled and

safe to handle with clean nitrile gloves, the glassware

can be restocked in appropriately labeled locations and

is ready for use. Nitrile gloves, safety glasses, lab coat.

0% 3 0.20 255

GEN II

‐

C1 Add 9.3

‐ 42 Kg trioctylphosphine oxide (TOPO), 1.6

‐ 9.6

Kg tetradecylphosphonic acid (TDPA), 4.3

‐ 20 L Cd Stock

in a 100 gallon reactor vessel. Heat to 250 C in 30

minutes, purge for 30

‐ 90 minutes, reduce purge and

ramp temperature to 300 C Closed tank system process using EZI

‐

DOCK loading bags (<1ppb I air) and

Cd Stock and TOP liquid transfer

system: nitrile gloves, safety glasses,

lab coat 0% 1 0.25 255

GEN II

‐

C2 Closed tank system using drop tanks

for additions to reactor: nitrile gloves,

safety glasses, lab coat. 0% 1 0.25 255

GEN II

‐

C3 Closed tank system using drop tanks

for additions to reactor: nitrile gloves,

safety glasses, lab coat. 1 0.25 255

GEN II

‐

C4 Closed tank system using flexible SS

hose to transfer from reactor to

transfer tank: nitrile gloves, safety

glasses, lab coat; face shield used

during all hose connections and

disconnections. 1 0.25 255

GEN II

‐

C5 Closed tank system using flexible SS

hose to transfer from transfer tank to

TFF Feed Tank: nitrile gloves, safety

glasses, lab coat; face shield used

during all hose connections and

disconnections. 1 0.25 255

GEN II

‐

C6 TFF is operated until process meets specification; the

material is then pumped to another transfer vessel. Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 1 0.25 255

GEN II

‐

C7 Prepare 1 mL sample for QAQC testing. Determine

absorbance λ using the UV/vis spectrometer and

emission λ and distribution full width half max using the

fluorometer. Sample is prepared in glove box, taken out

in a closed cuvette to do a UV

‐ Vis reading. In fume

hood, a sample is made using wet hexane to prepare the

second reading, using the fluorometer. Fume hood, nitrile gloves

, safety

glasses, lab coat

1 0.50 255

GEN II

‐

C8 All tanks and lines are cleaned with a clean in place

system using Toluene at 50 C. This is a closed system and personnel

where lab coat, gloves, safety glasses,

and face shield with connecting and

discounting lines 0.01% of residual

quantum dots 1 0.25 255 Gen II Reactor Core Reaction Procedure

GEN II Reactor Core Tangential Flow Filtration (TFF) Process

GEN II Reactor Clean In Place System

PMN Page 9 Attachment

Section A--(2) Occupational Exposure

Page 2 of 3Nanosys

Worker Activity EPA SanitizedTask Worker Activity Personal Protective Equipment/Eng

Controls Phy Forms and % product # Workers

Exposed Max

(hrs/day) Duration

(days/yr)

GEN II

‐

C9 All Transfer tanks and flexible lines are taking to the

stationary pumped cleaning system using Toluene. This is a closed system and personnel

where lab coat, gloves, safety glasses,

and face shield with connecting and

discounting lines 0.01% of residual

quantum dots 1 0.25 255

End of Document

PMN Page 9 Attachment

Section A--(2) Occupational Exposure

Page 3 of 3