### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 1 of 15 Data Requirement: PMRA Data Code: 9.8.4 (TGAI) or 9.8.6 (EP)

EPA DP Barcode: 423458

OECD Data Point: IIA 8.12 (TGAI) and IIIA 10.8.1.1 (EP)

EPA Guideline: 850. 4100

Test material: Temik 15G Purity: 14.81%

Common name: Aldicarb

Chemical name: IUPAC: Not reported.

CAS name Not reported.

CAS No.: 116

- 06 -3

Synonyms: Not reported.

Primary Reviewer: Elizabeth Krupka Signature:

Staff Scientist, CDM Smith Date: 06 //22/15

Secondary Reviewer: Teri Myers Signature:

Senior Scientist, CDM Smith Date: 0 7 / 0 2 /1 5

Primary Reviewer: N.E. Federoff Signature:

Wildlife Biologist, USEPA/OPP/EFEDERB2 Date: 07/08/15

EPA PC Code 098301

CITATION: Martin, J.A

. Temik 15G - Seedling Emergence Test. Unpublished study performed by Smithers

Viscient

, Wareham, MA

. Report Number/Study Number: 14070.6101. Study sponsored by AgLogic, LLC. Study

completed September 15, 2014.

DISCLAIMER: This document provides guidance for EPA and PMRA reviewers on how to complete a data

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to terrestrial vascular

plants. It is not intended to prescribe conditions to any external party for conducting this study nor to establish

absolute criteria regarding the assessment of whether the study is scientifically sound and whether the study satisfies

any applicable data requirements. Reviewers are expected to review and to determine for each study, on a case- by

case basis, whether it is scientifically sound and provides sufficient information to satisfy applicable data

requirements. Studies that fail to meet any of the conditions may be accepted, if appropriate; similarly, studies that

meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of

factors related to the test methodology and results in determining the acceptability of the study.

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 2 of 15 EXECUTIVE SUMMARY :

The effect of Aldicarb (TEMIK 15G) on the seedling emergence of one monocot crop ( perennial ryegrass, Lolium

perenne ) and one dicot crop ( tomato, Lycopersicon esculentum ) was studied at nominal concentration s of 0

(negative control ) 2.1, 4.1, 8.3, 17 and 33 lb ai/A

. A ldicarb was applied as a granular formulation.

The growth medium used in the seedling emergence test was a mixture of 70% sand and 30% loam (pH and %

organic carbon were not reported; % organic matter 1. 9 %). On day 14 the surviving plants per pot were recorded

and cut at soil level for meas uring the plant height and dry weight.

Emergence in the negative control ranged from 75

- 100% for perennial ryegrass and from 50

- 100% for tomato.

There were no statistically adverse differences in the treated group compared to the negative control for any species.

The reviewer determined survival based on the number planted . Based on the reviewer’s results, survival in the

negative control group was 87.5 % for perennial ryegrass and 92.5% for tomato. Survival in the treatment groups

ranged from 70

- 95% for perennial ryegrass and from 82.5

– 95% for tomato.

No significant effects on height or weight were observed for either species studied.

The most sensitive monocot and dicot species could not be determined due to a lack of toxicity and because only

one monocot and one dicot species was st udied. The study resulted in a NO A EC value of 33 lb ai/A for both

perennial ryegrass and tomato. An EC/IC 25 could not be determined for either species.

There were no phytotoxic effects in this study for any species in the treatment groups compared to the negative

control.

Maximum Labeled Rate: 33 lb a

. i

. /A

Results Synopsis

Most sensitive monocot: Could not be determined

EC 50 /IC 50 : Could not be determined 95% C.I.: N/A

EC 25 /IC 25 : Could not be determined 95% C.I.: N/A

EC 05 /IC 05 : Could not be determined 95% C.I.: N/A

Slope: NC 95% C.I.: N/A

NO A EC: 33 lb a.i./A

Most sensitive dicot: Could not be determined

EC 50 /IC 50 : Could not be determined 95% C.I.: N/A

EC 25 /IC 25 : Could not be determined 95% C.I.: N/A

EC 05 /IC 05 : 33 lb a.i./A (tomato biomass) 95% C.I.: 8.73

- 74.1 lb a.i./A

Slope: NC 95% C.I.: N/A

NO A EC: 33 lb a.i./A

This study is scientifically sound and is classified as acceptable

.

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 3 of 15 Table 1 (Tier II studies). Summary of most sensitive parameters by species (lb ai/A).

Species Endpoint NO A EC EC 05 EC 25 EC 50 Perennial Ryegrass None 33 NC NC NC

Tomato None 33 NC NC NC

I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: This study was conducted in compliance with OPPTS 850.4 100 : Terrestrial

Plant Toxicity, Tier I (Seedling Emergence), April 1996 ; OPPTS 850.4 22 5:

Seedling Emergence, Tier II, April 1996; and OECD #208, Part 1 :

Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test, July

2006. The reviewer evaluated the study methods according to OCSPP

Guideline 850.4100

, January 2012

. There were some d eviations noted by

the reviewer

.

1

. The physico

- chemical proper ties of the test material were not reported

.

2

. The spray volume was not reported.

3

. The limit of quantitation was not reported.

4 . The study author did not report the soil pH, cation exchange capacity

, or soil moisture content.

COMPLIANCE: S igned and dated GLP, Quality Assurance and Data Confidenti ality

statements were provided. This study was conducted in compliance with

Principles of Good Laboratory Practice as published by the OECD [ 1998;

ENV/MC/CHEM(9 8)17] and EPA [1989; FR 48(230)] with the exception

that routine soil and water screening analyses were conducted at GeoLabs,

Inc., Braintree, Massachusetts using standard U.S. EPA procedures and are

considered facility records under Smithers Viscient's Standard Operating

Procedures.

A. MATERIALS:

1. Test Material Temik 15G

Description: Granular

Lot No./Batch No. : NWTEAXV179

Purity: 14.81 %

Stability of compound

under test conditions: Analytical determinations based on measured concentration of the test

material in the spray solution yielded a recovery of 97 to 130 % of nominal

(n = 5 ). The method validation study was performed prior to the start of the

definitive test and determined an average recovery of ca. 85% from soil

.

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 4 of 15 (OECD recommends chemical stability in water and light )

Storage conditions of

test chemicals: T he test material was stored at room temperature in its original container in

a dark , ventilated cabinet

.

Table 2. Physical/chemical properties of Aldicarb

.

Parameter Values Comments

Water solubility at 20  ̊ C Not reported

Vapor pressure Not reported

UV absorption Not reported

pKa Not reported

Kow Not reported

2. Test organism :

Monocotyledonous species: Perennial Ryegrass ( Lolium perenne

, Poaceae , Linn ). EPA recommends four

monocots in two families, including corn and a root crop, such as onion

.

Dicotyledonous species: Tomato ( Lycopersicon esculentum

, Solanaceae , Celebrity Hybrid ) ; EPA

recommends six dicots in four families, including soybean

.

Seed source: Perennial ryegrass obtained from Granite Seed, Lehi, Utah

. Tomato obtained from Burpee,

W. Atlee Burpee and Company, Warminster, Pennsylvania.

Historical % germination of seed: Perennial ryegrass, 95%; tomato, N/A

. Seed storage, if any: Seeds were stored refrigerated in the dark until use.

B. STUDY DESIGN:

1. Experimental Conditions

a. Limit test: None.

b. Range

- finding study : None

.

c. Definitive Study

Table 3: Experimental Parameters

- Seedling Emergence.

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 5 of 15 Parameters Seedling Emergence

Details Remarks

Criteria

Duration of the test 14

Recommended test duration is 14

- 21 days.

OECD recommends that the test be terminated no

sooner than 14 days after 50 percent of the control

seedlings have emerged

Number of

seeds/plants/species/ replicate Five replicate s (pots) were

maintained for perennial ryegrass

with 8 seeds per replicate and ten

replicates (pots) with 4 seeds per

replicate were maintained for

tomato. Ten seeds per replicate should be used.

OECD recommends a minimum of five seeds

planted in each replicate within 24 hours of

incorporation of the test substance. All seeds of

each species for each test should be of the same

size class. The seed should not be imbibed.

Number of replicates

Control:

Adjuvant control:

Treated:

Control:

Adjuvant control:

Treated: Perennial ryegrass:

5

N/A

5

Tomato:

10

N/A

10 Four replicates per dose should be used.

OECD recommends a minimum of four replicates

per treatment

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 6 of 15 Parameters Seedling Emergence

Details Remarks

Criteria

Test concentrations (lb a i/A)

Nominal:

Measured: 0 (negative control ), 2.1, 4.1, 8.3,

17 and 33 lb ai/A

Not provided Five test concentrations should be used with a dose

range of 2X or 3X progression

OECD recommends three concentrations,

preferably with application rates equivalent to 0.0

(control), 1.0, 10.0 and 100 mg substance per kg of

oven

- dried soil.

Method and interval of

analytical verification

LOQ:

LOD: Three q uality control samples

were prepared at nominal

concentrations of 0.200, 2.00 and

7.00 mg a.i./L in dimethyl

sulfoxide (DMSO)

. All samples

were analyzed for aldicarb suing

liquid chromatography with mass

spectrometry (LC/MS/MS)

. Method validation was condu cted

using a solid/liquid extraction

procedure.

Not reported

Not reported

Adjuvant (type, percentage, if

used) N/A

Test container (pot)

Size/Volume

Material: (glass/polystyrene) 12

- cm tall with a top diameter of

14 cm and a bottom diameter of

12 cm.

Polypropylene Non

- porous containers should be used.

OECD recommends that non

- porous plastic or

glazed pot be used.

Growth facility Greenhouse covered with

Lexan® (polycarbonate) panels

Method/depth of seeding Seeds were planted in pots

labeled to identify the test

species, treatment level, replicate,

and study number . Seeds were

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 7 of 15 Parameters Seedling Emergence

Details Remarks

Criteria

impartially selected and planted

in a circular pattern within each

to a depth of ca. 1

- 2 cm below

the surface of the soil.

Test material application

Application time including

the plant growth stage

Number of application s

Application interval

Method of application The test material was applied

af ter planting.

1

N/A

- single application

The test material was applied

directly to soil . The treated

support medium was mixed using

a Hobart mixer and then 1.2 kg

dry weight of the treated support

medium was added to each

replicate.

Details of soil used

Geographic location

Depth of soil collection

Soil texture

% sand

% silt

% clay

pH:

% organic carbon

CEC

Moisture at 1/3 atm (%) Sunny Nook Farm, Rochester,

MA

Not reported.

Sandy loam

95

2

3

Not reported

1. 9

Not reported

Not reported Soil mixes containing sandy loam, loam, or clay

loam soil with no greater than 2% organic matter

are preferable. Glass beads, rock wool, and 100%

acid washed sand are not preferred.

OECD prefers the soil to be sieved (0.5 cm) to

remove coarse fragments. Carbon content should

not exceed 1.5% (3% organic matter). Fine

particles (under 20um) makeup should be between

10 and 20%. The recommended pH is between 5.0

and 7.5.

Details of nutrient medium, if

used 100 mL of 400 mg/L Peters 20

-

20

- 20 solution

Watering regime and

schedules

Water source/type: Deionized water containing the

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 8 of 15 Parameters Seedling Emergence

Details Remarks

Criteria

Volume applied:

Interval of application:

Method of application: Peters 20

- 20

- 20 nutrient solution;

all other watering conducted

using w ell water.

Not reported.

Nutrient containing water applied

twice weekly; f

requency of well

water not stated.

The plants were sub

- irrigated

. EPA prefers that bottom watering be utilized for

seedling emergence studies so that the chemical is

not leached out of the soil during the test.

Any pest control

method/fertilization, if used Seeds had not been pretreated

with fungicides or insecticides.

Test conditions

Temperature:

Photoperiod:

Light intensity and quality:

R elative humidity: 16

- 39°C (day); 15

- 26°C (night)

16L:8D

81

- 630 PAR, μmol/m 2 /sec

20

- 97 % EPA prefers that the cold vs warm loving plants be

tested in two separate groups to optimize plant

growth.

OECD prefers that the temperature, humidity and

light conditions be suitable for maintaining normal

growth of each species for the test period.

Reference chemical (if used)

Name:

Concentrations: Aldicarb

Not reported.

Other parameters, if any None

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 9 of 15 2. Observations:

Table 4: Observation Parameters

- Seedling Emergence.

Parameters Seedling Emergence

Details Remarks

Parameters measured (e.g., number

of germinated seeds, emerged

seedlings, plant height, dry weight

or other endpoints) - Emergence

- Survival

- Height

- Individual d ry weight

- Phytotoxicity

Measurement technique for each

parameter Emergence and

phytotoxicity were

visually determined.

Survival was defined as

the percent of emerged

plants by the study author

. Shoot length was

determined by measuring

t he length of the longest

leaf for monocots and by

measuring to the tallest

new growth for dicots

. Average replicate dry

weight was determined by

cutting shoots at the soil

surface and placing them

in a n individually labelled

container for drying in an

oven at ca 70 o C for at

least three days before

weighing.

Observation intervals Each pot was o bserved on

days 0. 7, and 14 for

emergence and

phytotoxicity

. Shoot

length and d ry weight was

recorded at study

termination

.

Other observations, if any N/A

Were raw data included Yes

Phytotoxicity rating system, if used 0

– indicates no injury or

abnormality; 100

- complete effect (dead

plant).

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 10 of 15 II. RESULTS and DISCUSSION:

A. INHIBITORY EFFECTS:

1. Seedling Emergence:

Emergence in the negative control ranged from 75

- 100 % for perennial ryegrass and from 50

- 100% for

tomato

. There were no statistically adverse differences in the treated group compared to the negative

control for any species . The reviewer obtained the same results.

The study author calculated survival based on number of emerged. Because no plants died after e merging,

survival in both the negative control and the treatment groups was 100%. The reviewer determined survival

based on the number planted . Based on the reviewer’s results, survival in the negative control group was

87.5 % for perennial ryegrass and fro m 92.5 % for tomato . S urvival in the treatment groups ranged was 70

-

95 % for perennial ryegrass and 82.5

– 95% for tomato

.

Neither the reviewer nor the study author observed any significant effects on height or weight for either

species studied.

The most sensitive monocot and dicot species could not be determined due to a lack of toxicity and because

only one monocot and one dicot species was studied. The study resulted in a NO A EC value of 33 lb ai/A

and EC 25 value of > 33 lb a.i./A for both perennial ryegrass and tomato.

There were no phytotoxic effects in this study for any species in the treatment groups compared to the

negative control.

B. REPORTED STATISTICS:

Fisher's Exact Test with Bonferroni

- Holm's Adjustment (U.S. EPA, 2002) was used to establish treatment

effects for percent emergence and percent survival data. Shoot length and shoot dry weight data were first

checked for normality using Shapiro

- Wilks' Test (U.S. EPA, 2002) and for homogeneity of variance using

Bartlett's Test (U.S. EPA, 2002). Statistical analysis to determine the NO A EC for each endpoint was carried out

using Dunnett’s Test for those data sets passing tests for homogeneity and normality. For those data sets that

were not homogenous and/or normal , the Steel Many

- One Rank Sum test was used to determine the NO A EC.

All analyse s were conducted at a 95% level of confidence, except for Shapiro

- Wilks' and Bartlett's Tests, where

the 99% level of certainty was applied. Statistical analysis was carried out using CETISTM (Ives, 2013)

.

Table 5: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for height (lbs ai/A)

height (cm) NO A EC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Ryegrass 14.7

- 16.4 33 ND N/A >33 N/A ND N/A N/A N/A

Tomato 7.07

- 9.44 33 ND N/A >33 N/A ND N/A N/A N/A

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 11 of 15 ND

- Not determined. N /A

- Not applicable.

Table 5a: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for biomass (lbs ai/A)

weight ( m g) NO A EC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Ryegrass 0.00934

- 0.0126 33 ND N/A >33 N/A ND N/A N/A N/A

Tomato 0.0446

- 0.0762 33 ND N/A >33 N/A ND N/A N/A N/A

ND

- Not determined. N /A

- Not applicable.

Table 5b: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for emergence (lbs ai/A)

% NO A EC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Ryegrass 70

- 95 33 ND N/A >33 N/A ND N/A N/A N/A

Tomato 82.5

-

92.5 33 ND N/A >33 N/A ND N/A N/A N/A

ND

- Not determined. N/A

- Not applicable

.

Table 5c: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for survival (lbs ai/A); based on # emerged

% NO A EC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Ryegrass 70

- 95 33 ND N/A >33 N/A ND N/A N/A N/A

Tomato 82.5

- 95 33 ND N/A >33 N/A ND N/A N/A N/A

ND

- Not determined. N/A

- Not applicable

.

Mid

- study emergence

Control Tomato Ryegrass Formulation

Blank

N R NR N R

NR

- Not reported

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 12 of 15 Plant Injury Index*

Control Tomato Ryegrass Formulation

Blank

0 0 0 NA

* 0

- No effect; 1 0 0 = complete effect, death of entire plant.

C. VERIFICATION OF STATISTICAL RESULTS BY THE REVIEWER:

The reviewer analyzed all endpoints by comparing the negative control with the treatment group using the equal

variance one

- sided t

- Test; if data were non

- parametric, the Wilcoxon Rank Sum test was used. These analyses

were conducted using CETIS version 1.8.7.12 and backend settings approved for use by EFED on 3/25/14. The

measured test concentrations were used in the analys is

.

Table 6: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for height (lbs ai/A)

height (cm) NO A EC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Ryegrass 14.7

- 16.4 33 NC N/A NC N/A NC N/A NC N/A

Tomato 7.07

- 9.44 33 NC N/A NC N/A NC N/A NC N/A

N C

- Not calculable . N /A

- Not applicable

.

Table 6a: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for biomass (lbs ai/A)

weight ( m g) NO A EC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Ryegrass 0.00934

-

0.0126 33 NC N/A NC N/A NC N/A NC N/A

Tomato 0.0446

- 0.0762 33 33 8.73

- 74.1 NC N/A NC N/A NC N/A

NC

- Not calculable. N/A

- Not applicable.

Table 6b: Effect of Aldicarb on 14

- Day Seedling Emergence

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 13 of 15 Species Results summary for emergence (lbs ai/A)

% NO A EC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Ryegrass 70

- 95 33 NC N/A NC N/A NC N/A NC N/A

Tomato 82.5

-

92.5 33 NC N/A NC N/A NC N/A NC N/A

NC

- Not calculable. N/A

- Not applicable.

Table 6c: Effect of Aldicarb on 14

- Day Seedling Emergence

Species Results summary for survival (lbs ai/A) ; based on # planted

% NO A EC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Tomato 82.5

- 95 33 NC N/A NC N/A NC N/A NC N/A

Ryegrass 70

- 95 33 NC N/A NC N/A NC N/A NC N/A

NC

- Not calculable. N/A

- Not applicable.

Mid

- study emergence

Control Tomato Ryegrass Formulation

Blank

NR NR NR

NR

- Not reported

Plant Injury Index*

Control Tomato Ryegrass Formulation

Blank

0 0 0 NA

* 0

- No effect; 1 0 0 = complete effect, death of entire plant.

Most sensitive monocot: Could not be determined

EC 50 /IC 50 : Could not be determined 95% C.I.: N/A

EC 25 /IC 25 : Could not be determined 95% C.I.: N/A

EC 05 /IC 05 : Could not be determined 95% C.I.: N/A

Slope: NC 95% C.I.: N/A

NO A EC: 33 lb a.i./A

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 14 of 15 Most sensitive dicot: Could not be determined

EC 50 /IC 50 : Could not be determined 95% C.I.: N/A

EC 25 /IC 25 : Could not be determined 95% C.I.: N/A

EC 05 /IC 05 : 33 lb a.i./A (tomato biomass) 95% C.I.: 8.73

- 74.1 lb a.i./A

Slope: NC 95% C.I.: N/A

NO A EC: 33 lb a.i./A

D. STUDY DEFICIENCIES:

There were no deficiencies that impacted the study

.

E. REVIEWER ’ S COMMENTS:

The results of the reviewer and study author were in agreement. The study only included on e monocot

species and on e dicot species; a lack of toxicity was observed for both species. The study resulted in a

NO A EC value of 33 lb ai/A for both perennial ryegrass and tomato. An EC/IC 25 could not be determined

for either species.

The in

- life portion of the test with was conducted from June 26 to July 23 , 2014

.

F. CONCLUSIONS:

This study is scientifically sound and is classified as acceptable

. T h e most sensitive monocot and dicot

species could not be determined due to a lack of toxicity and because only one monocot and one dicot

species was studied

. The study resulted in a NO A EC value of 33 lb ai/A for both perennial ryegrass and

tomato. An EC/IC 2 5 could not be determined for either species.

Most sensitive monocot and EC /IC 25 : Could not be determined

Most sensitive dicot and EC /IC 25 : Could not be determined

III. REFERENCES :

DeVellis, Stephen, 2014. Temik 15G (Alidcarb)

- Validation of the Analytical Method for the Determination of a

Test Substance in Soil. Smithers Viscient, Wareham, Massachusetts, Study No. 14070.6100

Ives, M., 2013. CETIS, Comprehensive Environmental Toxicity Information SystemTM. User's Guide. Tidepool

Scientific Softwar e, McKinleyville, California.

Norberg

- King, T. J., 1993. A Linear Interpolation Method for Sublethal Toxicity: The Inhibition Concentration

(ICp) Approach. National Effluent Toxicity Assessment Center, Environmental Research Laboratory

- Duluth, U.S. Envi ronmental protection Agency, Duluth, Minnesota. Technical report 03

- 93.

OECD, 1998. OECD Series on Principles of Good Laboratory Practice and Compliance Monitoring. Number 1.

OECD Principles on Good Laboratory Practice (as revised in 1997). Environment Directorate Chemicals

Group and Management Committee. ENV/MC/CHEM(98)17. OECD Paris. France. 41 pp.

OECD, 2006. OECD Guidelines for the Testing of Chemicals. Terrestrial Plant Test: 208: Seedling Emergence and

Seedling Growth Test.

### Data Evaluation Record on the Acute Toxicity of Aldicarb to Terrestrial Vascular Plants:

Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49477401

Page 15 of 15 U.S. EPA, 1989. Federal Insecticide, Fungicide and Rodenticide Act (FIFRA); Good Laboratory Practice Standards;

Final Rule (40 CFR, Part 160). Federal Register, 48 (230); 34052

-

34074.

U.S. EPA, 2002. Short

- Term Methods for Estimating the Chronic Toxicity of Effluents an d receiving Waters to

Freshwater Organisms. Fourth Edition. EPA

- 821

- R

- 02

- 013. Office of Water (4303T), U.S. Environmental

Protection Agency, Washington, D.C.

U.S. EPA, 2012. Office of Chemical Safety and Pollution Prevention. Ecological Effects Test Guide line, OCSPP

850.4100. Terrestial Plant Toxicity, Tier 1 (Seedling emergence).

EPA 712

- C

- 012

- 153. April 2012. U.S. Environmental Protection Agency, Washington, D.C.