# ~ 

# I AGENCY USE ONLY United Statea OMB C on trol No :**2060~226** 

### ENVIRONMENTAL PROTECTION AGENCY Expires: 8131120,. Washington, DC 20460 Dal e of Receipt: 

## SNAP INFORMATION NOTICE

For Significant New Alternatives

# Da**~~.YRa v(l**  

# aJ015 When completed send this form to : De le Review Completed : 

### Via US Posta l Service : Via Overn!ghl Delive[Y**Servi~ ;**  

### SNAP DOCUMENT CONTROL OFFICER SNAP DOCUMENT CONTROL OFFICER OFFICE OF AIR AND RADIAT ION , 6205 J U.S. EPA CB I Status : 

### U .S. EPA 1310 L STREET NW 1200 PENNSYLVANIA AVE NW 1 

### O

"' FLOOR (MC 6205J) WASHINGTON , DC 20460 WASHINGTON , DC 20005

Enter the total number of pages in your SNAP information Notice : 21 DOC<Jment Control Number 

### INTRODUCTION GENERAL I NSTRUCTIONS This form may be used to subm it information under the Significant New Alternatives Policy (SNAP) program for the rev iew of alternatives to class I and class II ozone-depleting substances (ODS) under section 612 of 

### the Clean Air Act. SubmHters are required to provide this information on new substitutes to assist the Agency in assessing the acceptability of chemicals or processes that are cons idered alternatives for ODSs . A separate notice must be filed for each alternative you are submitting . PAPER SUBMISSIONS : All paper submissions must be provided in three complete copies . If information is to be claimed as confidential , all confidential information must be excised from one of the copies , which will be placed i n the public docket; the other 

### two copies must include the confidential material. If no claims of confidentiality are made fo r the submission , all cop ies must be identical. CD-ROM SUBMISSIONS : The SNAP program also accepts subm iss ions via CD-ROM . If information is to be claimed as confidential , all confidential information must be excised from one of the files on the CD- ROM , which will be placed in the publ ic docket; the other file must inc lude the c onfidential material. The file with the confidential material must Include "conta ins CBI " in the file name . STEP -BY-STEP INSTRUCTIONS : The Instructions for the SNAP Information Notice and TSCNSNAP Addendum (" Instructions ") are designed to assist applicants in completing this form . The i nstructions provide step-by-step guidance on subm itting a SNAP notice , asserting confidentiality claims , completing the notice form . sector-specific data requirements , and submitt ing test data arid supplemental information . To faci litate Agency rev iew of alternatives, this form must be filled out as completely as possible . Please provide all i nformation requested to the extent that II is known or reasonably ascertainable . Make reasonable estimates if actual data is unavailable . TYPE OF NOTICE (Check only one box) Select the appropriate box identifyi ng the type of notice submitted : Manufacturer Submission : New alternative , Substance , or Formulation Petit ion : New alternative , Substance , or Formulation Petit ion : Revision of 

### the Existing SNAP list Petition : Request to Graridfather Use of 

### An Unacceptable Subst itute

Form 1265-07 (Revised 09/2011) OMB Control No .: 2060-0226 Previous ed itions are obsolete

### ~ 

# 0 0 IJ

### Part 1- GENERAL INFORMATION You must provide the identity of the alternative chemical or processes and the idenmy and percentages of all components in a blend and/or process, even if you claim the identity as confidential. You may authorize another person to submit the identity for you, but your submission will not be complete and review cannot begin until EPA receives this information . 

### Part II- ALTERNATIVE-sPECIFIC INFORMATION Please indicate the sector(s) for which you are submitting information on the substitute by checking the appropriate boxes : Refrigeration and Air Conditioning jg] Foam Blowing D Solvent Cleaning D Fire Extinguishing and Explosion Prevention D Sterilization D Aerosols D Adhesives, Coatings , and Inks D Tobacco Expansion 

## n Pesticides D You may need additional copies of Part II if there are several manufacturing, processing , or use operations that you will describe in this notice . P lease reproduce these sections as needed . If your substitute is also subject to review under FIFRA, provide the FIFRA Registration Number:



### CONFIDENTIALITY CLAIMS Part Ill- RELEASE AND EXPOSURE DATA Please reproduce additional copies of Part Ill as necessary. 

### Part IV- 

### LIST OF ATTACHMENTS Please attach additional sheets if you do not have enough space on the form to fully answer a question . Label each continuation sheet with the corresponding section heading and the question. In part IV, list these attachments and any test data, other data, or any optional information that you include in this notice. 

### TEST DATA AND OTHER DATA You are required to submit all test data in your possession or control and to provide a description of all other data known to you if these data are related to the health and environmental effects of the manufacture , processing, distribution in commerce , use, or disposal of the alternative . Standard literature citations may 

### be submitted if they do not appear in the open scientific literature . Complete test data (in English) , not summaries of data , must be submitted if they do not appear in the open literature . The following are test data and other data that might be submitted (see the Instructions for a more complete list of data to be submitted .) Please indicate test data to be included in your submission notice by checking the appropriate boxes : Health Effects Data u Yes jg] No Flammability Data o Yes jg] No Environmental Effects Data o Yes jg] No Environmental Fate Data o Yes jg] No Risk Assessments o Yes jg] No Test Data Not in the Possession/ Control of the Submitter o Yes jg] No Other Data o Yes**~No**

Anyone submitting information must assert a claim of confidentiality at the time of submission for any data which are to be treated as Confidential Business Information (CBI) . Substantiation of this claim must also 

### be provided at this time . All i nformation claimed as CBI will 

### be treated in a manner consistent with 40 CFR Part 2 , Subpart B . Failure to assert a claim of confidentiality at the time of submission may result in disclosure of information by the Agency without further notice . To assert a claim on this form , ci rcle or bracket the information you claim as confidential and mark the confidential box at the bottom of the page . Then provide substantiation of this claim on an attachment to the notice . If any information is claimed as confidential , you must provide a "sanitized " version of this notice , including attachments, to EPA with your submission. For additional information on claiming information as confidential, please see the Instructions Manual . Information submitted as CBI may be accessed by companies designated as Authorized Representatives of the United States Environmental Protection Agency (EPA) under an EPA contract for the purpose of assisting EPA in the development and implementation of national regulations for the protection of stratospheric ozone, including the development of the SNAP program . These Authorized Representatives may have access to any information received by the Stratospheric Protection Division within the EPA Office of the Atmospheric Programs for use in reviewing the need for possible control of any substance, practice , process or activity that may reasonably be anticipated to affect stratospheric ozone . Access to such information is necessary to ensure that these compan ies can complete the 

### work required by the contract. Authorized Representatives of the Administrator are subject to the provisions of 42 U .S . C . 7414(c) respecting confidential business information as implemented by 40 CFR 2 .301(h) . Form 1265-07 (Revised 09/2011) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 2

## Part I- GENERAL INFORMATION Section A - SU B M ITTE R ID E NTIFI C ATION I. (a) Person Submilling No lice (in U .S. )**~ike $..** **attan** President & CEO Name ofAu thorized Official Ti tl e 

### EllP!lru!o.rf .J r

w . Company/O rganization 

### 175 Freshwater Boulevard , Enfield , C I 

### 06082-4444 +1 .860 .253 .6625 Ma i li ng Address Telephone & Fax Nu mbe rs**sa tt** **an m@el!~n** **rf om**  E-mai l Address (b) Agent (if applicable) Name ofAuthorized Official Title Company/Organization Mailing Address Telephone & Fax Numbers



## -- - E-mail Address (c) Joint Submitter (If applicable) Dr. Wilhelm**PI ~~H** **er** BQl!rl:!**mem!;)~r Q**  Name of Authorized Official Title E; ppendorf AG Co mpany/O rgan izati on Barldlausenweg 

### 1 , 22339 Hamburg , German'J' 

### +49 40 53801 644 Mailing Ad d ress Telephone & Fax Numbers**[l lueste** **w@em ~~** **ndorf de**  E-mail Address 2. Technical Contact (In U. S. ) 

### Dr. Thomas Uschkureit Executive Director Name of Authorized Official Title E[l[lendorf Inc . - Company/Organization 

### 17 Q**Fr ~§** **hwate r**  Bo ulevard , Enfield , CT 

### 06082-4444 +1 . 860 .253 .6623 Mailing Ad dress Telep hone & Fax Nu mbers**!; hk** **r~i! !@~ll** **ll~!l Q[f** **!;;Q m**  E- mail Address 3. 

### Ifyou have had a prior communiculiun wilh EPA cuncernlng this nutlce, nule the clute Hnt.ltype ur cummuniculiutr (feller, phune, etc .) and the EPA staff person's name: Telephone conversation on the 

### 12"' August 

### 2014 (10 am Eastern time) between Margaret Sheppard and Rebecca Vondemhagen (both EPA) ; and Mary Lisa Sassano (Product Manager Eppendorf Inc . USA) , AI Les (Safety Officer Eppendorf Inc. USA) , Mike King (Managing Director Eppendorf CryoTech Ltd UK) , Dr. Thomas Uschkureit (Executive Director Portfolio Management Eppendorf Inc. USA) , Goran Micic (Design Engineer Eppendorf CryoTech Ltd UK) , David Minister (Consultant Eppendorf CryoTech Ltd UK) . Mark (X) i f None

Fonn 

### 1265-07 (Revised 

### 0912011) OMB Control No . : 

### 2060-0226 Previous editions are obsolete 

## n Mark (X) this box if this page contains CBI

_ _ __

## 0 3

### Part I- GENERAL INFORMATION (continued) Section B- ALTERNATIVE IDENTIFICATION (Continued)



# ------------------- ---- 5. Impurities

(a) Identify each impurity that may reasonably be anticipated to 

### be present in the chemical alternative as manufactured for commercial purposes If there are unidentified impurities, enter "unidentified."

(b) Provide the CAS Registry Number for each impurity if available. (c) Estimate the maximum weight percentage ofeach impurity If there are unidentified impurities, estimate their total weight percentages R170: (a) Unidentified (b)N/A (c) N/A R290: (a) Unidentified 

### (b) N/A (c) N/A

6. Byproducts :

(a) Describe any byproducts or degradation products resulting from the manufacturer, processing, use or disposal of the chemical alternative or chemicals used in the new alternative . If there are unidentified byproducts/ degradation products enter "unidentified."

(b) Provide the CAS Registry Number for each byproduct or degradation product ifavailable. (c) Indicate where the byproduct/degradation product is formed (e.g. during manufacturing, during use, in case offire, etc) and the amount or rate at which it is formed R170: (a) Carbon dioxide (C02) and water (H20) (b) 124-38-9 and 7732-18-5 (c) Assuming complete combustion in the case of fire, the following is produced :

2C2H6 

### + 702 --+**4C~** 

### + 6H20 ; 0 .221b (98g) of substitute refrigerant creates 0.631b (287 .5g) of C02 and 0 . 391b (176 .4g) of H 2 0 R290: (a) Carbon dioxide (C02) and water (H2 0) (b) 124-38-9 and 7732-18-5 (c) Assuming complete combustion in the case of fire, the following is produced :

C3He 

### + 502--+ JC02 

### + 4H20; 0 .261b (120g) of substitute refrigerant creates 0 .791b (360g) of**C~** and 0.431b (196g) of H20

Form 1265-07 (Revised 09/2011) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 6 Mark (X) this box if this page contains CBI _____

## 0

### Part 

## II - ALTERNATIVE-SPECIFIC INFORMATION (Continued) Section 8- ATMOSPHERIC INFORMATION I . Ozone-depletion potential (ODP):

(a) Provide the 100 year ODP ofthe alternative relative to CFC-11 , ifknown . Ifthe substitute is a blend, provide the OOPs ofthe individual constituents . Reference the source for each ODP.

{b) Provide any additional data on the ODP of the alternative (e .g. atmospheric lifetime, chlorine or bromine loading potentials) . Reference the source ofthis information and attach any supporting documentation 

### .B1Z.Q;, (a) ODP 

# = 0 (http :1/www .linde-gas .com/en/products _and_supplylrefrigerantslnatural_refligera nts/R 170 _ethan eli ndex.html) (b) Unknown 

### R290: (a) ODP 

# = 0 (http :llwww.epa .govlozonelsnaplsubsgwps.html) (b) Unknown

Form 1265-07 (Revised 0912011) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 102 . Global Warming Characteristics: (a) Provide the alternative's atmospheric lifetime and global warming potential (GWP) relative to carbon dioxide over 100-year time horizon, and 20-year or 500-year time horizon, ifknown Reference the most recent assessment report of the Intergovernmental Panel on Climate Change (IPCC AR4) Alternate sources may include the 2010 World Meteorological Organization (WMO) Scientific Assessment of Ozone Depletion or the peer-reviewed literature. Ifthe substitute is a blend, provide the GWPs ofthe individual constituents and an estimate ofthe blend at its nominal composition.

(b) If known, provide the alternative's expected impact on energy efficiency relative to the substance it is replacing (e.g.+/- X%) and results of any testing or modeling done (both theoretical and actual testing) Energy efficiency information is particularly useful for refrigerants and foams

(c) For GWP values that do not come from AR4 or WMO 2010, provide any available additional data on the atmospheric lifetime and GWP ofthe alternative, including infrared absorption spectrum and infrared absorption capacity Reference the source ofthis information . R170: (a) 100 year GWP 

# = 5.5 (w.vw .iocc chroubllcations and dala/ar4/Wctllenlch2s2- t()..3-2.htrnl); 20 and 500 year GWP 

### Is unknown (b) Eppendorf manufactures a range of hydrocarbon 

### only and HFC only freezers . Hydrocarbon substitute tends 

## to be more efficient 

### in all Eppendorf freezers when compared 

### to the equivalent HFC unillf one is to examine 

### the latest data catalogue (as of 03/Sep/201li) , for exam le : 

### I FREEZER MODEL Hydrocarbon 

## I HFC I Energy reduct ion I 

## I U725 (G) 12.8kWhlday (230V 

### 50Hz version) 

## I 15 .8kWh/day (208V 60Hz version) 

## I 19% 

## _I 

## l CryoCube F570h 8 .7 kWh/day (230V, 50Hz version) 

## I 9.2kWhlday (115V , 60Hz version) 

## I 6% --- , In all of the above instances , cabinets have identical level of insulahon . Therefore , 

### power consumption reduction can be directly 

### attributed 

## to refrigerant and the hermetic compressor working in combination . 

### (c) 

### NIA

Form 1265-07 (Revised 09/2011) OMB Control No. : 2060-0226 Previous editions are obsolete Mark (X) this 

### box if this page contains CBI ____

## IJ

11

## Part II - ALTERNATIVE-SPECIFIC INFORMATION (continued) Section C · OTHER STATUTES AND STANDARDS I-- - -- -------- -

1. Describe whether and how the manufacture, use. and disposal ofthe alternative is regulated under other environmental regulatory statutes such as those listed below. List any concentration-based or other numerical standards to which the alternative is subject under the standards and regulations . Tttles of the Clean Air Act (CAA) other than Title VI R170 :

### N/A~ _ __,_, R

..,_ 29,0"'-:-'-N""/A'-'-1

- 

### the Clean Water Act (CWA) -'-' R,_,1..!..7,__0:,_, N"'"/""A'---"R,.,2,9"'0:._,N"'"/ "" A

'-- the Safe Drinking Water Act (SOWA)**-- ~R.!.17.u** **..!: !!!/** **C!._**

### _**_....,R~29..,0'-' ..!:N!!J/Ao._** **_** 

### the Resource Conservation and Recovery Act (RCRA) _, R _,_,_

17,_,0.., :..:.N,I.:..A,__ __ R:.=2,90,.,: '-'Nc.:./

c:. A ,_

_ the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) . R170 : NIA R290: N/A the Toxic Substances Control Act (TSCA) --'R'-'-'-17 :.: 0 .,.:.,

L ., is..,ted,.,___ _,_R,.,2,.,9_,0,_ : L.,i,st,.e,_d the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA)**-'-R.:..1'-'7_,0"':-'-N"'/A"'----'R -"** **"' ...,** **~:**

### .... N "" / '- A ,___ the Emergency Planning and Community Right to Know Act (EPCRA or SARA Title lll) _ _cR'-'-'-17,_,0.., :..:.N"'/'-'A'- 2

_ 

### __,R.,2..,9"'0"- :.:..;N,../A_, 2

_ 

### State and local laws R170 : Unknown Other applicable environmental federal, state, and local laws not mentioned above --"R'-'1"-7-" 0 '-:,U"'n,k!..!.nO,;W=n___ R...,.,_2,90"':'-'U=nk.,nceo,w=n 1 This product does not contain any substances regulated as hazardous air pollutants (HAPS) under Section 112 of the Clean Air Act 

### Amendments of 1990 2 Not applicable since amount used is under 10 000 pounds . Actual annual amount used in production will be signifocantly less than 1000 pounds for each substitute - R 170 and R290 . 

### The intended use is for very low temperature refrigeration. This application limits the amount of proposed substitutes to 150 gram per refrigeration circuit. In regard to disposal of the substitutes from the freezers, common practice is to recover the refrigerant substnute and return it to the supplier for disposal.

Form 1265-07 (Revised 0912011) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 122 Describe whether and how occupational, consumer, or general population exposure to the alternative is regulated under health and safety related statutory authorities, such as those implemented by the Food and Drug Administration (FOA), the Occupational Safety and Health Administration (OSHA), the Department ofTransportation (OOT), and state and local laws List any concentration-based or other numerical standards to which the alternative is subject under the above statutes and regulations . Also list any other standard-setting organizations such as the National Fire Prevention Association (NFPA) the American Society of Heating, Refrigerating, and Air Conditioning Engineers (ASHRAF.), or Underwriters Laboratories (UL) that will evaluate the substitute from a health and safety perspective. R170: -FDA: not listed -OSHA (relevant sections) : 1910.94- Ventilation; 1910 . 106- Flammable liquids; 1910.110- Storage and handling of liquefied petroleum gases; 1910 . 133- Eye and face protection; 1910 . 138- Hand Protection; 1910 . 1000 TABLE Z-1- TABLE Z-1 Limits for Air Contaminants- not listed; OSHA Permissible Exposure Limit (PEL): Construction Industry- Simple Asphyxiant (Note 

### 3);

- DOT: unknown -NFPA: possible relevancy of NFPA 497 (2012 edition) standard - Recommended Practice for the Classification of Flammable Liquids, Gases or Vapors and of Hazardous (Classified) Locations for Electrical Installations in Chemical Process Areas -ASH RAE : 15-2013, Safety Standard for Refrigeration Systems is applicable -UL: UL471 standard is applicable to commercial refrigerators and freezers (equal or under 600V AC) employing hermetic or semi-hermetic compressors and air/water-cooled condensers. This standard takes into consideration the use of hydrocarbon refrigerants. However, it is unclear if ethane is recognized as a refrigerant. In addition, the standard also covers laboratory refrigerators and freezers but it is uncertain if Very Low Temperature Freezers fall into this end-use category . 

### R290: -FDA: TITLE 21, CHAPTER I, SUBCHAPTER B, Part 184, Section 184.1655 Propane- recognized as generally safe to use in foods intended for human consumption -OSHA (relevant sections) : 1910.94- Ventilation; 1910.106- Flammable liquids; 1910 . 110- Storage and handling of liquefied petroleum gases; 1910.133- Eye and face protection; 1910.138- Hand Protection ; OSHA Permissible Exposure Limit (PEL)=> Generallndustry => 29 CFR 1910 . 1000 Z-1 Table --1000 ppm, 1800 mg/m3 TWA; -DOT: unknown -NFPA: possible relevancy of NFPA 497 (2012 edition) standard- Recommended Practice for the Classification of Flammable Liquids, Gases, or Vapors and of Hazardous (Classified) Locations for Electrical Installations in Chemical Process Areas -ASHRAE: 15-2013, Safely Standard for Refrigeration Systems is applicable -UL: UL471 standard 

### is applicable to commercial refrigerators and freezers (equal or under 600V AC) employing hermetic or semi-hermetic compressors and air/water-cooled condensers. The standard takes into a consideration the use of hydrocarbon refrigerants, with propane being accepted as one ofthem . The standard also covers laboratory refrigerators and freezers but it is unclear if Very Low Temperature Freezers fall into this end-use category .

Form 1265-07 (Revised 09/2011) OMB Control No .: 2060-0226 Previous editions are obsolete 

## 13 Mark (X) this box if this page contains CBI I]

## Part II- ALTERNATIVE-SPECIFIC INFORMATION (continued) Section D- COST AND AVAILABILITY OF THE ALTERNATIVE I Estimate the cost per pound for the substitute chemical . For alternative processes and technologies, describe installation costs associated with the alternative . Please describe the information used as the basis for this cost estimate 

### R170: Cost of substitute is $70.32/lb (£43.41 per pound at the time of writing). There is no additional cost associated with the alternative. R290: Cost of substitute is $12.20/lb (£7 .53 per pound at the time of writing). There is no additional cost associated with the alternative . 2 Provide information on production ofthe substitute . Include estimates on :

(a) Year substitute will be available ifnot currently produced . (b) Total pounds production per year (current figure). (c) Years to maximum market penetration (d) Maximum total production per year at market saturation . (e) Estimated percentage or market at market saturation 

### R170: (a) Currently available . (b) Unknown . (c) Very low usage, limited to very low temperature refrigeration. (d) Unknown. (e) Unknown. R290: (a) Currently available. (b) Unknown . (c) Estimate is 

### 10 years . (d) Unknown . (e) Unknown. 3 . Describe any new equipment and use profiles that will be necessary in order to use the substitute If retrofitting ofexisting equipment is required, detail changes in technologies needed to use the alternative and address any materials compatibility issues . Provide information on each end-use and capital costs associated with the substitute or alternative process . Include cost ofequipment and/or any new materials, equipment lifetime, changes in labor and energy costs. 

### R170: End users should not require any new equipment or use profiles in order to use the freezers containing proposed substitute . R290: End users should not require any new equipment or use profiles in order to use the freezers containing proposed substitute.

Form 1265-07 (Revised 09/2011) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 14 Mark (X) this box if this page contains CBI

____ 

## II

## Part III- RELEASE AND EXPOSURE DATA Section A- TOXICITY AND HAZARD INFORMATION For chemical alternatives and for chemicals used with alternative processes, summarize below (and attach supporting information on) the acute and chronic toxicity of the substitute and of its constituent chemicals on any organism (e g human and/or other mammals, fish , wildlife, and plants), if available . Include any data on impurities and degradation products ifknown Also include information on any permissible exposure limits (PELs), occupational exposure limits (OELs), or Acceptable Exposure Limits (AELs) that have been set for the substitute fo r use in the workplace . In addition, please attach a copy of any haZBrd warning statement, label, material safety data sheet, or other information which will be provided to any person who is reasonably likely to be exposed . (See Pgs . 16-17 ofthe Instructions for sector-specific requirements for toxicity testing) 

### R170: 

### Acute toxicity : no known information ; Chronic Toxicity: no known infonnation; Exposure through inhalation : may cause central nervous system depression with nausea, headache, dizziness , vomiting, and incoordination . High concentrations may also cause cardiac sensitization resulting in irregular heartbeat. Treatment folowing 

### the exposure : Conscious inhalation victims should be assisted to an uncontaminated area and inhale fresh air. If 

### breathing Is difficult, administer oxygen . Unconscious persons should be moved to an uncontaminated area and, as necessary, given artificial resuscitation . Eco-toxiclty: Will 

### not bio-concentrate . Decomposition products are carbon monoxide (CO) and carbon dloldde**(C~) .**  

### Threshold limit value (Tl V) 

### proposed by 

### the ACGIH states 

### tine weighted average (TWA) of 1 OOOppm over 8 hours. NFPA dasslflcation : Health Hazard 2 , Flammability 4, Stability 0 HMIS classifiCation : Health Hazard 1, Rammability 4, Physical Hazard 3 Freezers containing 

### the substitute should be labeled with "Extremely flammable " sticker. 

### hltD lhnsds. Uncft1!5.corrv1i/eslmsd!tWP$ LIND 043 ETHANE NA MSDS FINAL REV 9 27 13odf**~** 

### Acute 

### toxicity: no known information; Chronic Toxicity: no known information; Exposure through Inhalation : May cause central nervous system depresslon with nausea, headache, dizziness, vomiting, and Incoordination . High concentratlons may also cause cardiac sensitization resulting In 

### irregular heartbeat. Treatment following the exposure : Conscious inhalation victims should be assisted to an uncontaminated area and Inhale fresh air. If 

### breathing Is difficult, administer oxygen . Unconscious persons should be 

### moved to an uncontaminated area and , as necessary, given artificial resuscitation and supplemental oxygen . Treatment should be symptomatic and supportive . Eco-toxicity: Wll 

### not bio-concentrate . Decomposition products are not known. Threshold limit value (TLV) 

### proposed by 

### the ACGIH states time weighted average (TWA) of 1000ppm over 8 hours . Other limHs : OSHA PEL (TWA: 1000 ppm), 

### NIOSH IDLH (IDLH : 2100 ppm, TWA: 1000 ppm) . NFPA dassification: Health Hazard 2, FlammabHity 4, Stability 0 HMIS classifiCation : Health Hazard 1, Flammability 4 , Physical Hazard 2 Freezers containing the substitute should be labeled with " Extremely flammable" slicker. 

### htto l/mScl$1indeus.comlfiles/msdsM'PS LIND 058 PROPANE NA MSDS FINAL REV 9 27 13.odf

Form 1265-07 (Revised 0912011) OMB Control No .: 2060-0226 Previous editions are obsolete 

## 15

## Section B- ENVIRONMENTAL RELEASE AND DISPOSAL AT MANUFACTURE For each manufacturing site (for manufacturing of substitutes and/or products containing substitutes) :

(a) IdentifY all site locations (i e ., name and address) for each chemical manufacturing process . (b) IdentifY all site locations for manufacturing of products using substitutes (e .g ., refrigerators containing the substitutes) (c) IdentifY the points ofrelease and/or exposure during the manufacture of : (i) the substitute and/or (ii) products containing the substitutes (d) Provide information on the magnitude ofrelease if avai l able, along with the media to which the substitute is released (e .g ., indoor air, outdoor air, water, land) 

### R170 : 

### (a) The substitute is purchased from a supplier {th i rd party) . (b) Eppendorf Cryotech , Bates Road , Maldon , Essex, CM9 5FA , UK (c) The substitute might be 

### potentially released (resulting in exposure) during charging of a second stage refrigerat ion ci rcui t. (d) Fraction of a gram per each freezer , during disconnection of charg ing lines (i ndoor air) . 

### .Bm.;. (a) The substitute is purchased from a supplier (third party) . (b) Eppendorf Cryotech , Bates Road , Maldon, Essex, CM9 5FA, UK (c) The substitute might be potentially released (resulting in exposure) during charging of a first stage refrigeratio n circu it. (d) Fract ion of a gram pe r each freezer , during disconnection of charg ing lines (indoor air) .

Form 1 265-07 (Revised 091201 1 ) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 16 Mark (X) th is box ifth is page contains CBI ____

## O

## Part Ill- RELEASE AND EXPOSURE DATA (continued) Section E- OCCUPATIONAL EXPOSURE AT END-USE For all end-use scenarios of the substitute (end-uses utilizing the substitute in products and/or processes) :

(a) Describe the activities in which workers may be exposed to chemicals at the end-use (i.e., during installation, maintenance , inspection, servicing, or use) Answer items (b) through (g) for each activity

(b) Describe any protective equipment or engineering controls used to protect workers (e.g., goggles , gloves, chemical hoods) (c) Indicate the physical form ofchemicals at the time of handling/exposure (c g, solid, liquid , gas) . (d) Estimate average and maximum numbers of workers involved in each activity . (e) Estimate average and maximum duration of activity for any worker, in terms of(l) hours/day and (2) days/year 

### (f) Enter the contact pathway (e .g, ingestion, inhalation, dermal) (g) Estimate the average and high-end exposure concentrations (separated by a/), being sure to provide units . Personal monitoring data may be required for some end uses (see pgs . 17-18 of Instructions) R170: 

### (a) Workers may be 

### exposed to the substitutes during servicing or certain aspects of repair work being undertaken on a Very low Temperature Refrigeration system . (b) Gloves , long sleeved shirt and safety goggles . (c) Gas (d) One worker in most cases . (e) Typically up to five minutes, on a single day in a year during regular servicing. In the case of refrigeration component(s) replacement due to a breakdown, work carried out might be over period of several hours . However, substitute handling will last around 15-20 minutes for the purpose of recovery and recharge of the refrigeration circuit. (f) 

### Potential inhalation and dermal contact. (g) During regular system servicing (connecting pressure gauges to check refrigeration system pressures), a fraction of a gram of the substitute will be 

### released. If any of 

### the refrigeration components are to be changed , approximately a few grams of the substitute might be released during circuit dry nitrogen purge due to inability to completely recover the substitute from the system . 

### R290: (a) Workers may be exposed to the substitutes during servicing or certain aspects of repair work being undertaken on a Very Low Temperature Refrigeration system . (b) Gloves, long sleeved shirt and safety goggles . (c) Gas (d) One worker in most cases. (e) Typically up to five minutes, on a single day in a year during regular servicing . In the case of refrigeration component(s) replacement due to a breakdown , work carried out might be over period of several hours . However , substitute handling will last around 15-20 minutes for 

### the purpose of recovery and recharge of the refrigeration circuit. (f) 

### Potential inhalation and dermal contact. (g) During regular system servicing (connecting pressure gauges to check refrigeration system pressures), a fraction of a gram of 

### the substitute will be released. If any of the refrigeration components are to be changed, approximately a few grams of the substitute might be released during circuit dry nitrOQen purge due to inability to completely recover the substitute from the system. 

## Section F- CONSUMER EXPOSURE For each end-use of a substitute (end-uses where a consumer might be exposed):

(a) Identify the potentially exposed consumer population (e .g, . indoor residents). (b) Identify the exposure medium (e .g ., air). (c) Enter the contact pathway (e .g, . ingestion, inhalation , dermal) (d) Describe the exposure point of concentration . Provide {I) average and (2) high-end concentration estimates, including units of measure . 

### Substitutes in question are not intended for a consumer (end-user) handling and usage .

Form 1265-07 (Revised 09/2011) OMB Control No .: 2060-0226 Previous editions are obsolete 

### 18

### Mark (X) th is box if this page contains CBI ____

## D

Part Ill- RELEASE AND EXPOSURE DATA (continued) Section G -GENERAL POPULATION EXPOSURE (optional) For manufacturing of substitutes and products containing substitutes:

(a) IdentifY and number pot ential general p opulation exposure points . (b) For each exposure point described in (a), identifY the potentially exposed population (e .g, residents at the fenceline to the manufacturing facility) .

(c) IdentifY the contact pathway ( e g , ingestion, inhalation, dermal). (d) IdentifY the exposure medium (e.g., air, water) . (e) Enter the exposure point concentration (mglm3 

### or ppb). Pr ovide ( 1) average and (2) high-end estimates .

Fonn 1265-07 

### (Revised 0912011) 

### OMB Control No .: 2060-0226 

### Previous editions are obsolete 

### 19 Mark (X) 

### this box If th is page contains CBI ____

## D

### Part 

## IV- LIST OF ATTACHMENTS List below any attachments that complete the responses to the questions on this form or that provide addi tional information that may assist EPA's review of the alternative under Section 612 of the Clean Air Act. Also, provide citations for information already submitted to EPA as part ofpast regulatory and information activities, as well as for other information that could not be included with this submission Ifyou have attached continuation pages, describe the Part, Section and question number being continued Attach additional pages if necessary. Mark (X) in the CBI box next to any attachment that contains information you claim as confidential. Attac hment Name Question# Number of Pages CBI



### Mark (X) this box if th is page conta i ns CBI ____

## I I Form 1265-07 (Revised 0912011) OMB Control No .: 2060-0226 Prev ious editions are obsolete 

## 20

## Part V- CERTIFICATION I certify to the best ofmy knowledge and beliefthat: I. 

### All information provided in this notice is complete and truthful as ofthe date ofthe submission . 2. I am submitting with this notice all test data in my possession or control and a description of all other data known to or reasonably ascertainable by me . 3 . 

### Ifthis is a submission ofa new alternative, the company named in Part I, Question Ia ofthis notice :

(a) intends to manufacture, formulate, import, market, or use a new alternative to a Class I or Class II ozone-depleting substance which is identified in Part I, Section B, Question 2.

(b) seeks an acceptability determination on a new alternative(s) to a Class I or Class II ozone-depleting substance, which is identified in Part I, Section B, Question 2. 

## 4. The accuracy ofthe statements made in this notice reflects my best prediction ofthe 

### anticipated facts regarding the alternative described herein . Any knowing and willful misinterpretation is subject to 

### criminal penalty pursuant to 

### section 113(c) ofthe 

### Clean Air Act and 18 U.S .C.§IOOI. 

## Signature and Title ofA•thorized Official

# (Orl**t;-x~ nd)'**  Date Mike Sattan ; President & 

### CEO Eppendorf 

### Inc. 

# d.

# J-Ar·'~ ;w,.( 

# ).) _**~**

### ~ 

# - Dr. Wilhelm Pluester; Board member, 

### CTO EppendorfM3**q;;v ~**  

# 2? 

# Ltarch**201~** Signature ofAgent (Where Applicable): Date For persons filing a SNAP Information Notice, the reporting burden is estimated to average 171 hours per year. For persons filing a TSCAISNAP Addendum, the reporting burden is estimated to average 36 hours her year. Burden means that total time, or financial resources expended by persons to generate, maintain, retain, or disclose or provide information to or for a Federal agency. This includes the time needed to review instruction; develop , acquire, install, and utilize technology and systems for 

## the purposes of collecting, validating, and verifying information; adjust the existing ways 

## to comply with any previously applicable instructions and requirements ; train personnel to be able 

## to respond to a collection of information; search data sources; complete and review the collection of information ; and transmit or otherwise disclose the information . 

## An agency may not conduct or sponsor, and a person Is not required 

## to respond to, a collection of information unless it displays a currently valid OMB control number. Send comments on the Agency's need for this information, the accuracy of the provided burden estimates, and any suggested methods for minimizing respondent burden, including through the use of automated collection techniques 

## to the Director, Collection Strategies Division (2822T), U .S. Environmental Protection Agency,1200 Pennsylvania Ave NVV, Washington, DC 20460 . Include 

## the OMB control number in 

### any correspondence . 

## Do not send the completed form to this address .

Form 1265-07 

### (Revised 0912011) OMB 

### Control No .: 2060-0226 

### Previous editions are obsolete 21