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# Abernathy Fish Technology Center

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                -  Abernathy Fish Technology Center
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## Library

Welcome to our Library!  This is a repository for Abernathy's published peer-reviewed journal articles, its internal reports, Abernathy's Fish Feed Quality Control Quarterly Reports, and Abernathy's PIT Pack Construction Manual. Scroll down to find them in that order. For a copy of any of these documents, contact Elden Roskelley at steven_dyer@fws.gov:
                          - Journal Articles
                          - Internal Reports
                          - Abernathy Fish Feed Quality Control Quarterly Reports
                          - PIT Pack Construction Manual

**JOURNAL ARTICLES**

All Fish Technology Centers (FTCs) are responsible for publishing at least one peer-reviewed paper per year, and most FTCs produce many more. The requirement is so negligible because publishing papers is more a means than an end for FTCs. The primary function of FTCs is to provide information that may be used to make scientifically based management decisions. Sometimes that information is very important, but not unique or complete enough to publish. Sometimes the information is so important, that it is obligatory to share it via a peer-reviewed paper. 

BELOW IS A LIST OF RECENT PUBLICATIONS WITH LINKS. CURRENT AND FORMER AFTC STAFF NAMES ARE IN BOLDFACE.

#### ***Conservation Genetics Publications***

**2023**

**Mussmann, S.M.**, A.S. Harrison, and W.D. Wilson.  2023.  Development of twenty‐one novel microsatellite loci for Gila topminnow, *Poeciliopsis occidentalis occidentalis*. Molecular Biology Reports. https://doi.org/10.1007/s11033-023-08376-7. 

**2022**

Whitesel, T.A., DeHaan, P.W., Doyle, J., **Adams, B.**, and Sankovich, P.M. (2022). Evaluating the success of a conservation reintroduction: The case of bull trout in the Wallowa River. Conservation Science and Practice, e12674. Link to article on non-FWS website.

**Kennedy, B.M.**,**Smith, M.J.**, Holmes, J.S.A.,**Root, R.P.**, **Smith, C.T.** and **Peterson, D.P.**(2022, Size and Distribution of Parr Produced from Natural- and Hatchery-Origin Steelhead Spawning Naturally in a Small Pacific Northwest Coastal Stream. Transactions of the American Fisheries Society. Link to article on non-FWS website.

**2021**

**Bohling, J., J. Von Bargen, M. Piteo,**A. Louden,M. Small, T.A. Delomas, and R. Kovach. 2021. Developing a Standardized Single Nucleotide Polymorphism Panel for Rangewide Genetic Monitoring of Bull Trout. North American Journal of Fisheries Management 41(6):1920-1931.

**2020**

**Bohling, J**. Evaluating the effect of reference genome divergence on the analysis of empirical RADseq datasets. Ecology and Evolution. 10:7585-7601. Link to article on non-FWS website.

Fraser, G.S., P.W. DeHaan, **C.T. Smith**, **J.F. Von Bargen**, M.R. Cooper and T.J. Desgrosseillier. 2020. Overlap of Spatial and Temporal Spawning Distributions of Spring and Summer Chinook Salmon Results in Hybridization in the Upper Columbia River. Transactions of the American Fisheries Society: 149:517-531. Link to article on non-FWS website.

**2019**

**Bohling, J., J. Von Bargen** and P. Bahls. Genomic Characterization of Coho Salmon Spawning Populations from the Hood Canal. Transactions of the American Fisheries Society. 149:3-13. Link to article on non-FWS website.

Bohling, J., M.P. Small, **J. Von Bargen**, A. Louden, and **P. DeHaan**. 2019. Comparing inferences derived from microsatellite and RADseq datasets: a case study involving threatened bull trout. Conservation Genetics 20(2)329-342. Link to article on non-FWS website.

**Bohling, J.H.**, S. Starcevich, **J. Von Bargen**, and E.J. Bailey. 2019. Describing fine-scale patterns of genetic structure **structure**** Something temporarily or permanently constructed, built, or placed; and constructed of natural or manufactured parts including, but not limited to, a building, shed, cabin, porch, bridge, walkway, stair steps, sign, landing, platform, dock, rack, fence, telecommunication device, antennae, fish cleaning table, satellite dish/mount, or well head.  Learn more about structure  and introgression of Redband Trout in a complex river system. North American Journal of Fisheries Management 39:509-523. Link to article on non-FWS website.

Habicht, C., **C.T. Smith**, A. Barclay, H.A. Hoyt, K. Turnquist, and W.A. Larson. 2019. Discriminating among Pacific salmon, Rainbow Trout, and Atlantic Salmon species using commonly available genetic screening methods. Journal of Fish and Wildlife Management 10(1):228-240. Link to article on non-FWS website.

Jordan, G.R., E.J. Heist, B.R. Kuhajda, G.R. Moyer, P. Hartfield, and **M. Piteo**. 2019. Morphological Identification Overestimates the Number of Pallid Sturgeon in the Lower Mississippi River due to Extensive Introgressive Hybridization. Transactions of the American Fisheries Society 148(5):1004-1023. Link to article on non-FWS website.

**Smith, C.T., J. Von Bargen, P.W. DeHaan**, P. Scheerer, and M.H. Meeuwig. 2019. Genetic structure and the history of chub in the Alvord Basin. Conservation Genetics 20(3):489-501. Link to article on non-FWS website.

**2018**

**Bohling, J.H.**, T.A. Whitesel, and M. Brown. 2018. Genetic characteristics of coastal cutthroat trout inhabiting an urban watershed. Environmental Biology of Fishes 101(5):799-811. Link to article on non-FWS website.

Cunningham, C.J., T.A. Branch, T.H. Dann, **M.J. Smith**, J.E. Seeb, L.W. Seeb, and R. Hilborn. 2018. A General Model for Salmon Run Reconstructions That Accounts for Interception and Differences in Availability to Harvest. Canadian Journal of Fisheries and Aquatic Sciences 75(3):439-451. Link to article on non-FWS website.

Saglam, I.K., J. Baumsteiger, **M.J. Smith**, J. Linares-Casenave, A.L. Nichols, S.M. O'Rourke, and M.R. Miller. 2018. Best available science still supports an ancient common origin of Devils Hole and Devils Hole pupfish. Molecular Ecology 27:839-842. Link to article on non-FWS website.

Silver, B.P., J.M. Hudson, **C.T. Smith**, K. Lujan, M. Brown, and T.A. Whitesel. 2018. An urban stream can support a healthy population of coastal cutthroat trout. Urban Ecosystems 21(2):291-304. Link to article on non-FWS website.

**2017**

**Bohling, J.H.**, L.L. Mastro,. J.R. Adams, E.M. Gese, S.F. Owen, and L.P. Waits. 2017. Panmixia and Limited Interspecific Introgression in Coyotes (*Canis latrans*) from West Virginia and Virginia, USA. Journal of Heredity 108:608-617. Link to article on non-FWS website.

**Piteo, M.S.**, M.R. Kendrick, and P.M. Harris. 2017. Life History of Labidesthes vanhyningi (*Atheriniformes: Atherinopsidae*; Stout Silverside) in the Black Warrior River Drainage, Alabama. Southeastern Naturalist 16(3):451-463. Link to article on non-FWS website.

**2016**

**Bohling, J.H.** 2016. Strategies to address the conservation threats posed by hybridization and genetic introgression. Biological Conservation 203:321-327. Link to article on non-FWS website.

**Bohling, J.H**., J. Dellinger, J.M. McVey, D.T. Cobb, C.E. Moorman and L.P. Waits. 2016. Describing a developing hybrid zone between red wolves and coyotes in eastern North Carolina. USA. Evolutionary Applications, 9(6):791-804. Link to article on non-FWS website.

**Dehaan, P., B. Adams**, P. Scheerer, and B. Bangs. 2016. Influence of Introduction History on Genetic Variation in Introduced Populations: A Case Study of Oregon Chub. North American Journal of Fisheries Management, 36:1278-1291. Link to article on non-FWS website.

**Dehaan, P., J. Von Bargen**, and P. Scheerer. 2016. Genetic variation and the relationship between stream and lake exotypes of a threatened desert Catostomid, the Warner sucker (*Catostomus warnerensis*). Ecology of Freshwater Fishes 26(4):609-620. Link to article on non-FWS website.

Lesica, P., **B. Adams**, and **C.T. Smith**. 2016. Can physiographic regions substitute for genetically-determined conservation units? A case study with the threatened plant, Silene spaldingii. Conservation Genetics 17:1041-1054. Link to article on non-FWS website.

Saglam, I.K., **J. Baumsteiger**, **M.J. Smith**, J. Linares-Casenave, A.L. Nichols, S.M. O'Rourke and M.R. Miller. 2016. Phylogenetics support an ancient common origin of two scientific icons: Devils Hole and Devils Hole pupfish. Molecular Ecology 25(16): 3962-3973. Link to article on non-FWS website.

**Smith, C.T.**, **B. Adams**, M. Barton, M.K. Burnham-Curtis, E. Monroe, J.B. Olsen, W.D. Wilson, A. Williams, M.J. Millard, M.A.H. Webb, and J.K. Wenburg. 2016. Comment on Haig et al. (2016): The conservation genetics juggling act: integrating genetics and ecology, science and policy. Evolutionary Applications 9:635-637. Link to article on non-FWS website.

**2015**

Kovach, R.P.M., C.C.: Wade, A.A.: Hand, B.K.: Whited, D.C.: **DeHaan, P.W**.: Al-Chokhachy, R.: Luikart, G. 2015. Genetic diversity is related to climatic variation and vulnerability in threatened bull trout. Global Change Biology 21(7):2510-2524. Link to article on non-FWS website.

**Smith, C.T., J. Baumsteiger, M.R. Ardren**, Y. Dettlaff, **D. Hawkins**, and D.M. Van Doornik. 2015. Eliminating variation in age-at-spawning leads to genetic divergence within a single coho salmon population. Journal of Fish and Wildlife Management 6(1)4-18. Link to article on non-FWS website.

**Von Bargen, J.**, J. Rueth, and **C.T. Smith**. 2015. Development of a Chinook Salmon Sex Identification SNP Assay based on the Growth Hormone Pseudogene. Journal of Fish and Wildlife Management 6(1):213-219. Link to article on non-FWS website.

#### ***Nutrition & Physiology Publications***

**2022**

**Gannam, A. **2022. Conservation Aquaculture: A special sessions synopsis. World Aquaculture Magazine 53: 20-29.

**2021**

Blouin, M.S., Wrey, M.C., Bollmann, S.R., Skaar, J.C., **Twibell, R.G**. and Fuentes, C., 2021. Offspring of first generation hatchery steelhead trout (*Oncorhynchus mykiss*) grow faster in the hatchery than offspring of wild fish but survive worse in the wild: Possible mechanisms for inadvertent domestication and fitness loss in hatchery salmon. Plos ONE, 16(12), p.e0257407.

**2020**

**Barron, J., **R.G. **Twibell, K.M. Wing**, and **A.L. Gannam**. 2020. Evaluation of Practical Diets for Juvenile River Suckers. North American Journal of Aquaculture 82:169-180.

**Barron, J.M., K. Hanson, R. Headley, K. Hawke, R. Twibell**, and **A.L. Gannam**. 2020. Evaluation of effluent waste water from salmonid culture as a potential food and water supply for culturing larval Pacific lamprey (Entosphenus tridentatus). Aquaculture 517 (2020):734-791. Link to article on non-FWS website.

**2018**

**Barron, M.J.**, **R.R. Headley**, **A.K. Hawke**, **J.S.A. Holmes**, **A. Carr,****K. Strailey**, and **A.L. Gannam**. 2018. Optimization of culture temperatures for larval Pacific lamprey. World Aquaculture Magazine September 2018, pp. 58-62.

**2017**

Eya, J.C., R. Yossa, D. Perera, O. Okubajo, and **A.L. Gannam**. 2017. Combined effects of diets and temperature on mitochondrial function, growth and nutrient efficiency in rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology, Part B 212: 1-11. Link to article on non-FWS website.

**Hanson, K.C**. and **J.M. Barron**. 2017. Evaluation of the effects of marking Pacific lamprey ammocoetes with visual implant elastomer, coded wire tags, and passive integrated transponders. Transactions of the American Fisheries Society 146: 626-633. Link to article on non-FWS website.

**Twibell, R.G., A.L. Gannam, J.M. Barron, K. Wing, K.C. Hanson, D.P. Peterson**, B.F. Balmer, and D. Nelson. 2017. Effects of dietary lipid and light source on steatitis in steelhead, *Oncorhynchus mykiss*. Aquaculture 479:370-377. Link to article on non-FWS website.

**2016**

**Barron, J.M., R.G. Twibell, **and A.L. Gannam. 2016. Evaluation of first feeds for larval Lost River Suckers. North American Journal of Aquaculture 78:92-100. Link to article on non-FWS website.

**Barron, J.M., Twibell, R.G.**, Hill, H.A., **Hanson, K.C.** and **Gannam, A.L**. 2016. Development of diets for the intensive culture of Pacific lamprey. Aquaculture Research 47: 3899-3906. Link to article on non-FWS website.

**Hanson, K.C., R.G. Twibell, R.A. Glenn, J.M. Barron**, and **A.L. Gannam**. 2016. The effects of a transition diet on the smolitification of Chinook salmon. North American Journal of Aquaculture 78:307-313. Link to article on non-FWS website.

Koch, J.F., S.D. Rawles, C.D. Webster, V. Cummins, Y. Kobayashi, K.R. Thompson, **A.L. Gannam, R.G. Twibell,** and **N.M. Hyde.** 2016. Optimizing fish meal-free commercial diets for Nile tilapia. *Oreochromis niloticus*. Aquaculture 452: 357-366. Link to article on non-FWS website.

Lampman, R., M.L. Moser, A.D. Jackson, R.K. Rose, **A.L. Gannam**, and **J.M. Barron**. 2016. Developing techniques for artificial propagation and early rearing of Pacific Lamprey (Entosphenus tridentatus) for species recovery and restoration. Chapter 22, pp. 160-195. A.M. Orlov and R.J. Beamish, editors. Jawless Fishes of the World. 2 volumes. Cambridge Scholars Publishing, Cambridge, UK. Link to article on non-FWS website.

**Twibell, R.G**., **J.M. Barron**, and **A.L. Gannam**. 2016. Evaluation of Dietary Lipid Sources for the Juvenile Lost River Sucker. North American Journal of Aquaculture 78(3): 234-242. Link to article on non-FWS website.

Webster, C.D., S.D. Rawles, J.F. Koch,. K.R. Thompson, Y. Kobayashi, **A.L. Gannam**, **R.G. Twibell**, and **N.M. Hyde**. 2016. Bio-Ag reutilization of distiller's dried grains with solubles (DDGS) as a substrate for black soldier fly larvae. Hermetia illucens, along with poultry by-product meal and soybean meal, as total replacement of fish meal in diets for Nile tilapia. *Oreochromis niloticus.* Aquaculture Nutrition 22:976-988. Link to article on non-FWS website.

**2015**

Eya, J.C., V.O. Ukwuaba, R. Yossa, and **A.L. Gannam**. 2015. Interactive effects of dietary lipid and phenotypic feed efficiency on the expression of nucelar and mitochondrial genes involved in the mitochondrial electron transport chain in rainbow trout. International Journal of Molecular Sciences 16:7682-7706. Link to article on non-FWS website.

Eya, J.C., V.O. Ukwuaba, R. Yossa, M.F. Ashame, C.F. Pomeroy, and **A.L. Gannam.** 2015 Growth performance and mitochondrial function in juvenile rainbow trout (*Oncorhynchus mykiss*) fed graded dietary lipid levels. Annals of Aquaculture and Research 2(1):1006-1019. Link to article on non-FWS website.

**Gannam, A.L**. 2015. B-Glucans. Chapter 5 of Dietary Nutrients, Additives and Fish Health. C.S. Lee, C. Lim, D.M. Gatlin and C.D. Webster, editors. John Wiley & Sons, Inc. Hoboken, N.J. Link to article on non-FWS website.

**Glenn, R.A., P.W. Taylor**, E.H. Pelton, S. Gutenberger, M. Ahrens, L.M. Marchant, and **K.C. Hanson**. 2015. Genomic evidence of vertical transmission and cycling of the enteric redmouth disease (*Yersinia ruckeri*) organism in Tule fall chinook salmon *Oncorhynchus tsawtscha*. Journal of Fish and Wildlife Management 6:44-54. Link to article on non-FWS website.

Kobayashi, Y., C.D. Webster, K.R. Thompson, V.C. Cummins, Jr.,**A.L. Gannam. R.G. Twibell, N.M. Hyde**, and J.F.A. Koch. 2015. Effects on growth survival, body composition, processing traits and water quality when feeding a diet without vitamin and mineral supplements to Australian red claw crayfish (Cherax quadricarinatus) grown in ponds. Aquaculture Research 46:2716-2727. Link to article on non-FWS website.

#### ***Quantitative Ecology & Technology Publications***

**2023**

**Peterson, D.P., R. Twibell, and M. Piteo. **2023.Retention of passive integrated transponder tags in hatchery brook trout: Effect of tag size, implantation site, and double tagging.  Fisheries Management and Ecology. https://doi.org/10.1111/fme.12616. 

**Hemeon, K.H.**, E.N. Powell, S.M. Pace, R. Mann, and T.E. Redmond. 2023. Population dynamics of *Arctica islandica* of Long Island (USA): an analysis of sex‐based demographics and regional comparisons.  Marine Biology 170:3.4 https://doi.org/10.1007/s00227-023-04176-6. 

Klinck, J.M., E.N. Powell, **K.H. Hemeon**, J.R. Sower, and D.R. Hennen. 2023. A Growth Model for *Arctica islandica*: The Performance of Tanaka and the Temptation of Von Bertalanffy - Can the Two Coexist? Journal of Shellfish Research 42(10): 77-90. https://doi.org/10.2983/035.042.0108. 

**2022**

**Peterson, D., **Pearson, J. and Simpson, W. (2022). Effects of common carp on water quality and submerged vegetation: results from a short-term mesocosm experiment in an artificial wetland. Marine and Freshwater Research 73(7): 973-994. 

**2021**

**Peterson, D.P., **and R.O. Engle. 2021. Ingestion of PIT Tags by Hatchery-Reared Juvenile Steelhead and Subsequent Detection at Main-Stem Dams in the Columbia River Basin. North American Journal of Fisheries Management 41(3): 871-885. Link to article on non-FWS website.

**2019**

**Peterson, D.P.** and N.M. Neville. 2019. Comparison of methods to verify upstream passage by trout at remediated culverts in four Rocky Mountain streams. North American Journal of Fisheries Management. 39:738-752. Link to article on non-FWS website.

**2018**

**Simpson, W.G., D.P. Peterson, K. Steinke**, and L. Beck. 2018. The efficacy of killing developing common carp embryos with electricity: using a laboratory evaluation to assess a potential means of reducing the recruitment of an invasive fish. Management of Biological Invasions 9(3):279-290. Link to article on non-FWS website.

**Simpson, W.G.**, 2018. The entertainment and screening of returning and postspawning adult salmonids at irrigation canals of the Umatilla River, Oregon. Journal of Fish and Wildlife Management 9(1): 285-295. Link to article on non-FWS website.

**2017**

Roberts, J.J., K.D. Fausch, M.B. Hooten, and **D.P. Peterson**. 2017. Nonnative trout invasions combined with climate change **climate change**** Climate change includes both global warming driven by human-induced emissions of greenhouse gases and the resulting large-scale shifts in weather patterns. Though there have been previous periods of climatic change, since the mid-20th century humans have had an unprecedented impact on Earth's climate system and caused change on a global scale.  Learn more about climate change  threaten persistence of isolated Cutthroat Trout populations in the southern Rocky Mountains. North American Journal of Fisheries Management 37(2): 314-325. Link to article on non-FWS website.

Tatara, C.P., M.R.  Cooper, **W. Gale, B.M. Kennedy**, C.R. Pasley, and B.A. Berejikian. 2017. Age and Method of Release Affect Migratory Performance of Hatchery Steelhead. North American Journal of Fisheries Management 37:700-713. Link to article on non-FWS website.

**2016**

**Simpson, W.G., DP. Peterson** and **K. Steinke**. 2016. Effect of waveform and voltage gradient on the survival of electroshocked steelhead embryos and larvae. North American Journal of Fisheries Management 36:1149-1155. Link to article on non-FWS website.

#### **INTERNAL REPORTS**

BELOW ARE OUR INTERNAL REPORTS , LISTED BY RESEARCH PROGRAM. CURRENT AND FORMER AFTC STAFF NAMES ARE IN BOLDFACE.

#### ***Conservation Genetics Internal Reports***

**2021**

**Nehmens, M. C.**, **R. Twibell** and **C. T. Smith**. 2021. Effects of treating fish with MS-222 and formalin on downstream genetic analyses.  AFTC Final Report.

**Smith, C.T., J. Von Baren, J. Bohling,**D. Hand, and I. Jezorek. 2021. Hybridization between historically allopathic Chinook Salmon populations in the White Salmon River, WA. AFTC Final Report. Link to Report on non-FWS website.

**Adams, B.** 2021. Genetic Analysis and Genetic Estimation of Spawner Abundance of Bull Trout Collected in the Lewis River, WA. AFTC Final Report.

**Bohling, J.H.** 2021. Genetic Analysis of Wild and YY Brook Trout Populations in New Mexico. AFTC Final Report.

**Smith, C.T., M. Nehmens, J. Von Bargen,** and **D. Peterson**. 2021. Evaluation of available genetic tools for monitoring the efficacy of Trojan male Brook Trout as an invasive species **invasive species**** An invasive species is any plant or animal that has spread or been introduced into a new area where they are, or could, cause harm to the environment, economy, or human, animal, or plant health. Their unwelcome presence can destroy ecosystems and cost millions of dollars.  Learn more about invasive species  control mechanism in Tyee Springs, WA. AFTC Final Report.

**Von Bargen, J.** 2021. Genetic Identification of Endangered Winter-Run Chinook Salmon in the Sacramento River, CA  2020 Annual Report.

**Adams, B., M. Piteo**, and **J. Von Bargen**. 2021. Genetic Analysis of Native Salmonids from the Lake Pend Oreille and Clark Fork River System, Idaho and Montana. Annual Report CY20.

**2020** 

**Smith, M.J., J. Von Bargen, C.T. Smith, M.R. Miller, J. Rasmussen, and D.A. Hewitt**. 2020. Characterization of the genetic structure of four sucker species in the Klamath River Basin. AFTC Final Report. Link to Report on non-FWS website.

**Adams, B.** 2020. Lake Pend Oreille Stock Identification of Bull Trout from 2006-2018. AFTC Final Report.

**Bohling, J.H.**2020. Genetic evaluation of out-migrating juvenile spring and summer Chinook Salmon in the Entiat River. AFTC Final Report.

**Smith, C.T.**, and **M. J. Smith.** 2020. Origin of Spring Chinook salmon used as broodstock **broodstock**** The reproductively mature adults in a population that breed (or spawn) and produce more individuals (offspring or progeny).  Learn more about broodstock  at Warm Springs NFH. AFTC Final Report.

**Smith, M.J.**, **M. Nehmens**, **C.T. Smith**, S. Reese and T. Becker. 2020. Relatedness among juvenile spring Chinook salmon with cataracts from Winthrop National Fish Hatchery. AFTC Final Report:14.

**2019**

**Adams, B**., and **C. Smith**. 2019 Genetic stock identification of Wenatchee River Bull Trout collected during 2014-2015. AFTC Final Report.

**Adams, B., M. Piteo**, and **J. Von Bargen**. 2019. Genetic analysis of native salmonids from the Lake Pend Oreille and Clark Fork River system, Idaho and Montana. AFTC Final Report CY 2018.

**Bohling, J.H.** 2019. Genetic assessment of Bull Trout in the Upper Willamette River basin, OR. AFTC Final Report. Link to Report on non-FWS website.

**Bohling, J.H**. 2019. Genetic characteristics of juvenile steelhead (*Oncorhynchus mykiss*) collected from the Bull Run River and the surrounding Sandy River basin, OR. AFTC Final Report. Link to Report on non-FWS website.

**Bohling, J.H.**, and **M. Piteo** 2019. Genetic monitoring of Bull Trout reintroduced to the Clackamas River, OR. AFTC Final Report. Link to Report on non-FWS website.

**Bohling, J.H.**2019. Testing alternative methods to collect genetic samples from juvenile steelhead. AFTC Final Report. Link to Report on non-FWS website

**Piteo, M.** and **J.H. Bohling.** Assessing the genetic origin of Redband Trout (Oncorhynchus mykiss gairdneri) occuphing Tumalo Creek and Bridge Creek in central Oregon. AFTC Final Report. Link to Report on non-FWS website.

**Piteo, M., J. Von Bargen** and **B. Adams.** 2019. Genetic species ID of putative Bull Trout collected in the Wallowa River system, OR. AFTC Final Report.

**Von Bargen, J.** and **C.T. Smith**. 2019. Genetic identification of endangered Winter-run Chinook Salmon in the Sacramento River, CA. AFTC Final Report.

**2018**

**Adams, B.** and J. Doyle. 2018. Genetic analysis and genetic estimation of spawner abundance of Bull Trout collected in the Lewis River, WA. AFTC Final Report FY 2017.

**Adams, B.**,  **M. Piteo**, and **J. Von Bargen**. 2018. Genetic analysis of native salmonids from the Lake Pend Oreille and Clark Fork River system, Idaho and Montana. AFTC Final Report CY2017.

**Bohling, J.**, **J. Von Bargen**, and P. Bahls. 2018. Genetic profile of North Hood Canal Coho Salmon using RAD sequencing. AFTC Final Report. Link to Report on non-FWS website.

**Smith, C.T.**, **J. Von Bargen**, P. DeHaan, P. Scheerer, and M.H. Meeuwig. 2018. Genetic structure of Chub in the Alvord Basin. AFTC Final Report.

**Smith, M.J**., **J. Von Bargen**, and G. Mendez 2018. Genetic determination of stock of origin for Oncorhynchus mykiss collected in the upper Deschutes River basin. AFTC Final Report.

**Von Bargen, J.** and **C.T. Smith**. 2018. Genetic identification of endangered Winter-run Chinook Salmon in the Sacramento River, CA.  AFTC Final Report.

**2017**

**Adams, B**., and J. Doyle. 2018. Genetic analysis and genetic estimation of spawner abundance of Bull Trout collected in the Lewis River, WA. AFTC Final Report FY 2017.

**Adams, B., B. Prom, C. Smith**, D. Brewer and J. Lindstrom. 2017. Population Assignment and Hybrid Status of Bull Trout Passed Above Barriers in Warm Springs Creek, MT. AFTC Final Report: 24.

**Adams, B., B. Prom**, and **J. Von Bargen**. 2017. Genetic Analysis of Native Salmonids from the Lake Pend Oreille and Clark Fork River System, Idaho and Montana. AFTC Final Report CY2016:49.

**Bohling, J.H.** 2017. Assessment of bull trout genetic diversity, populations, connectivity, and genetic introgression in the Upper Willamette Basin, OR. AFTC Final Report. Link to Report on non-FWS website.

**Bohling, J.H., J. Von Bargen**, and S. Starcevich. 2017. Evaluation of genetic population structure and effective population size among populations of redband trout in the Deschutes River, OR. AFTC Final Report. Link to Report on non-FWS website.

**DeHaan, P**., and S. Bernall. 2017. Bull Trout Parentage Analysis in the Prospect Creek Drainage, Montana. AFTC Final Report: 33.

**Smith, M.J., J. Von Bargen**, D.M. Faber, and W.H. Wilson. 2017. Investigation of the relative reproductive success of hatchery and wild steelhead in the Deschutes River Basin. AFTC Final Report  FY2016.

**Von Bargen, J. and C.T. Smith.** 2017. Genetic Identification of Endangered Winter-Run Chinook Salmon in the Sacramento River, CA. AFTC Final Report: 31.

**2016**

**Adams, B** and J. Doyle 2016. Rapid Response Genetic Analysis and Genetic Estimation of Spawner Abundance of Bull Trout Collected in the Lewis River, WA. AFTC Final Report FY2015: 25.

**Bohling, J.H**. 2016. Genetic profile for Little White Salmon NFH Spring Chinook salmon. AFTC Final Report FY 2016: 12.

**Bohling, J.H.** 2016. Genetic profile for Dworshak NFH Spring Chinook salmon. AFTC Final Report FY2016: 12.

**Bohling, J.H**. 2016. Genetic profile for Kooskia NFH Spring Chinook salmon. AFTC Final Report FY2016: 12.

**Bohling, J.H.** 2016. Genetic profile for Quinault NFH chum salmon. AFTC Final Report FY2016: 12.

**DeHaan, P., B. Adams, J. Von Bargen** and M. Brinkmeyer. 2016. Genetic Analysis of Native Salmonids from the Lake Pend Oreille and Clark Fork River System, Idaho and Montana. AFTC Final Report CY2015: 49.

**Smith, C.T., M. Smith**and D. Hand. 2016. Population genetic analysis of Parkdale Fish Hatchery Spring Chinook Salmon. AFTC Final Report.

**Smith, C.T., J. Von Bargen** and **M. Smith**. 2016. Genetic Characterization of late-returning coho salmon in the Tsoo-Yess River. AFTC Final Report: 28.

**Smith, M.J.**, **J. Von Bargen**, D.M. Faber and W.H. Wilson. 2016. Investigation of the relative reproductive success of hatchery and wild steelhead in the Deschutes River Basin. AFTC Final Report FY 20125: 26.

**Von Bargen, J**. and **C.T. Smith**. 2016. Genetic Identification of Endangered Winter-Run Chinook Salmon in the Sacramento River, CA. Final Report: 31.

**2015**

**Adams, B. and P. DeHaan. **2015. Rapid Response Genetic Analysis and Genetic Estimation of Spawner Abundance of Bull Trout Collected in the Lewis River, WA. AFTC Final Report FY2014:21.

**Adams, B., P. DeHaan**, and **C. Smith**. 2015. Genetic Determination of Stock of Origin for Oncorhynchus mykiss Collected in the Upper Deschutes River Basin. AFTC Final Report: 24.

**Bohling, J.H., C.T. Smith**, and T. Whitesel. 2015. Population structure of coastal cutthroat trout inhabiting urban watersheds in Portland, OR. AFTC Final Report FY2915: 28. Link to Report on non-FWS website.

**Bohling, J.H.**, and **C.T. Smith.** 2015. Genetic characterization coastal cutthroat trout from urban streams in Portland, OR. Final Report FY2016.

**DeHaan, P., B. Adams**, P. Scheerer, and B. Bangs. 2015. Genetic Monitoring and Evaluation of a Reintroduction Program for Oregon Chub. AFTC Final Report FY2015: 37.

**DeHaan, P., B. Adams,** B. Bangs, and P. Scheerer. 2015. Connectivity and Gene Flow among Oregon Chub Populations in the Middle Fork Willamette River. AFTC Final Report FY2015:37.

**DeHaan, P., B. Adams**, **J. Von Bargen**, and K. Wing. 2015. Genetic Analysis of Native Salmonids from the Lake Pend Oreille and Clark Fork River System, Idaho and Montana. AFTC Final Report CY2014: 49.

**DeHaan, P., B. Adams**, T. Whitesel, and J. Doyle. 2015. Genetic Species ID and Population Origin Analysis of Wallowa Lake and River Bull Trout. AFTC Final Report FY2015: 27.

**DeHaan, P., J. Von Bargen**, M. Meeuwig, and S. Clements. 2015. Great Basin Redband Trout Genetic Status Assessment. AFTC Final Report: 74.

**Smith, C.T., B. Adams**, K. Wing, **J. Von Bargen, M. Smith,** L. Earley, and M. Brown. 2015. Use of genetic data for life-history and monitoring analysis of Chinook salmon in Clear Creek and Battle Creek. AFTC Final Report.

**Smith, C.T**., and **J. Von Bargen**. 2015. Population structure of coastal cutthroat trout from Tryon Creek, OR. AFTC Final Report FY2015: 14.

**Smith, C.T., J. Von Bargen**, and K. Wing. 2015. Genetic assignment to run of steelhead smolts captured in the Bull Run River. AFTC Final Report FY 2015:13.

**Smith, M.J., J. Von Bargen**, D.M. Faber and W.H. Wilson. 2015. Investigation of the relative reproductive success of hatchery and wild steelhead in the Deschutes River Basin. AFTC Final Report FY2014: 18.

**Smith, M.J., J. Von Bargen**, and J. Rasmussen. 2015. Genetic information needs assessment for Lost River suckers (*Deltistes luxatus*) and shortnose suckers (*Chasmistes brevirostris*) of the Klamath River basin. AFTC Final Report FY2014: 46.

**Von Bargen, J.**, and **C.T. Smith**. 2015. Genetic Identification of Endangered Winter-Run Chinook Salmon in the Sacramento River, CA. Final Report FY2015:30.

**2014**

**DeHaan, P**., and **B. Adams**. 2014. Rapid response genetic analysis of bull trout collected in the Lewis River, WA. AFTC Final Report: 15.

**DeHaan, P., B. Adams**, and **J. Von Bargen**. 2014. Genetic analysis of native salmonids from the Lake Pend Oreille and Clark Fork River system, Idaho and Montana. AFTC Final Report: 41.

**DeHaan, P., B. Adams**, and J. Mogen. 2014. Genetic analysis of bull trout in the St. Mary River system, MT. Additional analyses for FY2013. AFTC Final Report: 18.

**DeHaan, P., B. Adams**, and M.C. Nelson. 2014. Fine-Scale Population Structure Analysis and Genetic Population Assignments of Wenatchee River Sub-Basin Bull Trout AFTC Final Report: 45.

**Smith, C.T.**, and **B. Adams**. 2014. Population genetic structure of Spalding's Catchfly. AFTC Interim Report.

**Smith, M.J.**, **J. Von Bargen**, D.M. Faber, and W.H. Wilson. 2014. Investigation of the relative reproductive success of hatchery and wild steelhead in the Deschutes River basin. AFTC Final Report FY 2013: 46.

**Von Bargen, J.**, and **C.T. Smith**. 2014. Genetic identification of endangered winter-run Chinook salmon in the Sacramento River, CA. AFTC Final Report FY2014: 5.

**2013**

**DeHaan, P., and B. Adams. **2013. Genetic species ID of putative bull trout collected in the Wallowa River system, OR. AFTC Final Report 3.

**DeHaan, P.**, and **B. Adams.** 2013. Rapid response genetic analysis of bull trout collected in the Lewis River, WA. AFTC Final Report: 15.

**DeHaan, P., B. Adams**, and **D. Hawkins**. 2013. Genetics Analysis of Native Salmonids from the Lake Pend Oreille and Clark For River System, Idaho and Montana. AFTC Final Report CY2012:36.

**DeHaan, P., B. Adams, D. Hawkins**, R. Tabor, and B. Thompson. 2013. Analysis of genetic variation in the Olympic Mudminnnow (novumbra hubbsi). AFTC Final Report: 47.

**DeHaan, P., D. Peterson, B. Adams**, and V. O'Byrne. 2013. Genetic monitoring of Arctic grayling in the Big Hole River and Red Rock Creek and Association with Recent Climate Trends. AFTC Final Report: 90.

**Smith, M.J., D. Hawkins**, D.M. Faber, and W.H. Wilson. 2013. Investigation of the relative reproductive success of hatchery and wild steelhead in the Deschutes River basin. AFTC Final Report FY2012: 49.

**Von Bargen, J., and C.T. Smith**. 2013. Genetic identification of endangered winter-run Chinook salmon in the Sacramento River, CA. AFTC Final Report FY2012: 5.

**2012**

**AFTC. **2012. Genetic profile for Makah NFH Winter Steelhead. United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report.

**AFTC**. 2012. Genetic profile for Quinault NFH Winter Steelhead. United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report.

**AFTC**. 2012. Genetic profile for Eagle Creek NFH coho slamon. AFTC Final Report: 14. 

**DeHaan, P.W.** 2012. Genetic analysis of Deadwood and Payette River bull trout. Final report for fiscal years 2010 and 2011.

**DeHaan, P.W.**, and **B. Adams**. 2012. Rapid response genetic analysis of bull trout collected in the Lewis River, WA. Final Report. U.S. Fish and Wildlife Service, Abernathy Center Final Report CY2011.

**DeHaan, P.W.**, **B. Adams,** **J. Von Bargen**, and **D. Hawkins**. 2021. Genetic analysis of native salmonids from the Lake Pend Oreille and Clark Fork River System, Idaho and Montana. United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report CY2011.

**DeHaan, P.W.**, and S. Bernall. 2012. Estimation of spawning success for bull trout transported above mainstem Clark Fork River dams. Final Report.

**DeHaan, P.W**., and J. Neibauer. 2012. Analysis of genetic variation within and among upper Columbia River bull trout populations. Final report.

**DeHaan, P.W., and J. Von Bargen.** 2012. Genetic analysis of threatened Warner suckers. Final Report.

**Smith, C.T.**, 2012. Genetic profile for Entiat NFH summer Chinook salmon. United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report.

**Smith, C.T.** and **J. Von Bargen**. 2012. Genetic profile for Warm Springs NFH spring Chinook salmon. United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report.

**Smith, M**. 2012. Genetic profile for Eagle Creek NFH coho salmon. AFTC Final Report: 14.

**Smith, M.**, **D. Hawkins**, **B. Adams**, and R. McCoun. 2012. Genetic characterization of coho salmon (Oncorhynchus kisutch) in Agency Creek, a tributary to the South Yahmill River (Willamette Basin, OR). United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report.

**Smith, M. J.**, **D. Hawkins**, and D. Faber. 2012. Investigation of the relative reproductive success of hatchery and wild steelhead in the Deschutes River basin. United States Fish and Wildlife Service, Abernathy Fish Technology Center Final Report.

**Smith, M.J.**, **J. Von Bargen**, and L. Denny. 2012. Genetic analysis of the origin of Chinook salmon in Panther Creek, Idaho. AFTC Final Report FY2012.

#### ***Nutrition and Physiology Internal Reports***

**2022**

**Gannam, A.**, **J. Chan**, **C. Slaugh** and J. Barron. 2022. Development of artificial propagation methods for production of juvenile Pacific lamprey (E*ntosphenus tridentatus*) for use in research associated with Section 4, 2, 3 of the Rocky Reach Pacific Lamprey Managment Plan. Annual Report prepared for the Chelan PUD, PUD No., 1 of Chelan County, 327 N. Wenatchee Ave., Wenatchee, WA  98807. April 2022.

**2019**

**Barron, J., R. Headley** and **A. Gannam**. 2019. 2019. Development of artificial propagation methods for production of juvenile Pacific lamprey (*Entosphenous tridentatus*) for use in research associated with Section 4.2.3 of the Rocky Reach Pacific Lamprey Management Plan.

**Twibell, R., N. Hams, K. Hawke**, and **S. Money**. 2019. Effects of a partial reuse aquaculture system (PRAS) on proximate composition and stress responses of brood year 2018 steelhead (Oncorhynchus mykiss) reared at Hagerman National Fish Hatchery. Prepared for Hagerman National Fish Hatchery.

**2018**

**Barron, J., R. Headley, K. Hawke, J. Holmes, A. Carr, K. Strailey** and **A. Gannam**. 2018. Development of artificial propagation methods for production of juvenile Pacific lamprey (*Entosphenus tridentatus*) for use in research associated with Section 4.2.3 of the Rocky Reach Pacific lamprey Management Plan. Annual Report. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center for Chelan PUD.

**Twibell, R., K. Strailey, A. Carr, K. Hawke**, and **R. Glenn**. 2018. Effects of a partial reuse aquaculture system (pRAS) on proximate composition and smoltification of brood year 2017 steelhead (Oncorhynchus mykiss) reared at Hagerman National Fish Hatchery. Prepared for Hagerman National Fish Hatchery.

**2017**

**Twibell, R**., Smith, C., Hanson, K. **Glenn, R.** , **Von Bargen, J.**, Bumgarner, J. and Clarke, L. 2018. Determination of gill Na+, K+-ATPase enzyme activity and gene expression from juvenile steelhead reared at Lyons Ferry and Irrigon Fish Hatcheries. AFTC Final Report.

**Barron, J.M.**, **K. Hawke**, **R. Twibell**, and **A. Gannam**. 2017. Development of artificial propagation methods of production of juvenile Pacific lamprey (*Entosphenus tridentatus*) for the use in research associated with Section 4.2.3 of the Rocky Reach Pacific Lamprey Management Plan. U.S. Fish and Wildlife. Abernathy Fish Technology Center.

**R.A. Glenn**, and K.C. Hanson. 2017. Temperature monitoring of Chinook salmon gametes during transfer from Little White Salmon National Fish Hatchery to Warm Springs National Fish Hatchery to Warm Springs National Fish Hatchery. Abernathy Fish Technology Center, US Fish and Wildlife Service.

**Twibell, R., R. Headley, R. Glenn**, and K. Hanson. 2017. Effects of a partial reuse aquaculture system (PRAS) on proximate composition and smolitification of BY2016 steelhead (*Oncorhynchus mykiss*) reared at Hagerman National Fish Hatchery. Prepared for Hagerman National Fish Hatchery.

**Twibell, R.G., C. Smith, K. Hanson, R.A. Glenn, J. Von Bargen,** J. Bumgarner and L. Clarke. 2017. Determination of gill Na+, K+-ATPase enzyme activity and gene expression from juvenile steelhead reared at Lyons Ferry and Irrigon fish hatcheries.

**2016**

**Barron, J., R. Twibell, A. Gannam **and K. Hanson. 2016. Development of artificial propagation methods for production of juvenile Pacific lamprey (Entosphenus tridentatus) for use in research associated with Section 4.2.3 of the Rocky Reach Pacific lamprey Management Plan. Annual Report. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center for Chelan PUD.

**Gannam, A.L., Twibell, R.G., Barron, J.M.**, and Wing, K.M. 2016. Diet Development for Lost River Suckers in the Klamath River Basin. AFTC Final Report.

**Twibell, R. and A. Gannam**. 2016. Effects of Partial Reuse Aquaculture System (PRAS) on Proximate Composition of Broodyear 2014 and 2015 Steelhead (*Oncorhynchus mykiss) *Reared at Hagerman National Fish Hatchery. AFTC Final Report.

**2015**

**Gannam, A.L., R.G. Twibell, and J.M. Barron. **2015. Diet development for Lost River suckers in the Klamath River Basin. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

**Twibell, R. and A. Gannam**. 2015. Effects of a Reuse Aquaculture System (RAS) on Proximate Composition of Steelhead (Oncorhynchus mykiss) reared at Hagerman National Fish Hatchery. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center: 10.

**2014**

**Gannam, A. R. Twibell and K. Hanson. **2014. Evaluation of the effects of an alternative feeding regime on the smolt status and body composition of juvenile spring Chinook salmon at Carson National Fish Hatchery. Final report. U.S. Fish and Wildlife Service, Abernathy Fish Technology for Carson NFH,

**2013**

**Davis, J., and A. Gannam.**2013. Investigation of contaminants in feeds and fish at FWS Pacific Region National Fish Hatcheries and the ramifications to human and ecological health. Final report. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

**Glenn, R.A.**, H. Hill, Ostrand S., **A. Gannam**, and K.C. Hanson. 2013. Intestinal microflorae of hatchery steelhead (Oncorhynchus mykiss) held in well water, surface water in the hatchery and in Abernathy Creek., WA. Internal report for Abernathy Fish Technology Center U.S. Fish and Wildlife Service.

**2012**

**Barron, J.M., R. Twibell**, H. Hill, M. Newson, and **A. Gannam**. 2012. Investigating the viability of using egg analogs to increase natural production of steelhead trout (*Oncorhynchus mykiss*). 1-16.

Conway, J., **R.G**. **Twibell, A.L.****Gannam**, and **N.M. Hyde**. 2012. Dietary phosphorous requirement of juvenile Gila trout (*Oncorhynchus gilae*). U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

Davis, J. and **A. Gannam**. 2012. Investigation of contaminants in feeds and fish at FWS Pacific Region national fish hatcheries and the ramifications to human and ecological health Annual report. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

**Gannam, A.L., R.G. Twibell**, H.A. Hill, **J.M. Barron, N.M. Hyde** and T.N. Pearsons. 2012. Development of low phosphorous and low leaching feeds for effluent management. Final report. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

**Glenn, R.A.** 2012. The development of a real-time PCR primer-probe set for the growth hormone 2 gene for use as an endogenous control in fish samples. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

**Hill, H.**, and **A. Gannam**. 2012. Comparing proximate and fatty acid composition of wild and captive humper lake trout eggs. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

**Hill, H.A.**, and **A.L. Gannam**. 2012. The U.S. Fish and Wildlife Service Pacific Region's fish feed quality control program: review of the past 10 years. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center.

#### ***Quantitative Ecology & Technology Internal Reports***

**2022**

**Hemeon, K.**, **Peterson, D**., **Twibell, R**., and**Piteo, M**. (2022). PIT tag detection in buried environments: a pilot study to evaluate PIT tag detection at varying depths and orientation. AFTC Project Report, 10 May 2022. 10 pp. 

**2021**

**Peterson, D.P., **J. Pearson, W. Simpson, and J. Barnett. 2021. Effects of common carp on water quality and submerged vegetation: results from a short-term mesocosm experiment in an artificial wetland. Final AFTC project report to Harney County Watershed Council and USFWS Malheur National Wildlife Refuge. 62 pp.

**2018**

**Peterson, Douglas P., Katherine Strailey**, and Michael Murray. 2018. Evaluating comparative PIT tag loss of steelhead reared in partial reuse aquaculture systems (PRAS) or traditional raceways at Hagerman National Fish Hatchery Annual Report 2018. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center, Longview, WA. 23 pp.

**Peterson, D.P., W.S. Simpson, and K.E. Steinke**. 2018. Evaluating comparative PIT tag loss of steelhead reared in partial reuse aquaculture systems (PRAS) or traditional raceways at Hagerman National Fish Hatchery Annual Report 2018. U.S. Fish and Wildlife Service, Abernathy Fish Technology Center, Longview, WA 29 pp.

**2017**

**Peterson, D.P., W.S. Simpson**, and L. Beck. 2017. Progress Report - Common Carp Biomass Threshold Experiment Pilot Study Work Conducted in Fall 2016. AFTC Interim Reports: 9 pp.

**Simpson, W.S., D.P. Peterson, K.E. Steinke**, and L. Beck. 2017. Efficacy of using electrofishing to reduce recruitment of invasive Common Carp in Malheur Lake, a large endorheic marsh. AFTC Final Report to Pacific Region Refuges (Invasive Species Control Project)

**2016**

**Simpson, W.S., and D. Peterson**. 2016 (A). Fisheries Monitoring System: Umatilla Project Facilities, Annual Report 2016. 48 pp.

**Simpson, W.S. and D. Peterson**. 2016 (B). Fisheries Monitoring System: Umatilla Project Facilities, Annual Report 2015. 47 pp.

**2015**

**Peterson, D.P.**, S. Bernall, K. Bouwens, K. Breidinger, J. DosSanto, D. Grupenhoff, W. Fredenberg, R. Kreiner, S. Moran, B. Naples, L. Nelson, R. Ryan, and D. Schmetterling. 2015. Strategic modeling to assist conservation of bull trout in the Lower Clark Fork River, 93 pp. + appendices.

Walsh, S.J., **D.P. Peterson**, and K.A. Techasarin. 2015. Mekong River Fish Ecology Information Gap Assessment and Capacity Building in Lao PDR. U.S. Department of Interior, International Technical Assistance Program, Final Report: 51 pp.

**2014**

**Simpson, W.S.** 2014. Cascades Pika Watch: Connecting People with Nature (CPWN) small project report. 6 pp.

**Simpson, W.S.** 2014. Fisheries Monitoring System: Umatilla Project Facilities, Annual Report 2014. 42 pp.

**2012**

**Hanson, K.C.** and **K.E. Steinke**. 2012. Fisheries monitoring system: Catherine Creek PIT-tag interrogation systems. Final Report. U.S. Bureau of Reclamation, Pacific Northwest Region, Boise, ID, 83706.

##### **FISH FEED QUALITY CONTROL QUARTERLY REPORTS**

Abernathy produces quarterly fish feed quality control reports and has for many years. To obtain a copy of a quarterly report, please submit a request to ann_gannam@fws.gov.

##### **PIT PACK CONSTRUCTION MANUAL**

To see our PIT Pack Construction Manual, click the adjacent link to view: VIEW

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