# Bioassays of Motiva Enterprises LLC – Port Everglades North Terminal Fort Lauderdale, Broward County, Florida NPDES #FL0000582 Sampled 3/25/02

November 2002



# Biology Section Division of Resource Assessment & Management Comprehensive Quality Assurance Plan #870346G

# Bioassays of Motiva Enterprises LLC – Port Everglades North Terminal Fort Lauderdale, Broward County, Florida NPDES #FL0000582

Biology Section Bureau of Laboratories November 2002

# Introduction Motiva Enterprises LCL – Port Everglades North Terminal, 909 SE 24 th 

# Street, Fort Lauderdale, Broward County, Florida, NPDES # 0000582, tests performed on 26 to 30 March 2002. This bulk petroleum storage terminal discharges effluent once to several times a month, with a flow range of 0.0075 to 0.359 MGD. The wastewater is not treated prior to discharge, and usually consists of storm water runoff and brackish ground water from an onsite pond. The wastewater is discharged into the Class III marine waters of the Intracoastal waterway (facility information provided by Stanley Ganthier, FDEP, West Palm Beach). The toxicity tests discussed in this report were performed in accordance with methods described by Weber, 1993, Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwaters and Marine Organisms , 4 th 

# Edition, EPA/600/4- 90/027F. 

# Results and Discussion The FDEP Biology Section performed two 48-hour static acute toxicity bioassays on a sample of effluent collected from the outfall of this facility. The test species used were the water flea, Ceriodaphnia dubia , and the bannerfin shiner, Cyprinella leedsi (Table 1). The sample was aerated for 45 minutes to increase the dissolved oxygen concentration from 3.8 to 8.0 mg/L. The C. leedsi test was terminated on March 27 after 24 hours due to excessive control mortality. The test was invalid due to 15% mortality of controls, while only 10% mortality is allowed in a valid test. This test was re-set on March 28 with the remaining bioassay sample. In the re-set bioassay, the 5% C. leedsi mortality in the 100% effluent was equivalent to the 5% mortality in the C. leedsi controls; therefore the sample mortality can’t be attributed to toxicity of the sample (Table 1). Total residual chlorine was not detected in the bioassay sample. Total ammonia and unionized ammonia were detected in the original bioassay sample at concentrations of 0.09 mg/L and 0.009 mg/L respectively. Total ammonia in the sample submitted for chemical analysis was 0.12 mg/L. When the C. leedsi test was re-set on March 28, ammonia was measured a second time and was not detected in the sample. Additional samples of effluent were collected from this facility and analyzed for herbicides, nutrients, metals, organic compounds, and pesticides. Several metals, two herbicides, and nutrients were detected (Table 2). 

# Conclusion The sample of effluent collected on March 25, 2002 from this facility did not show acute toxicity to C. dubia test organisms within 48 hours. The C. leedsi test was invalidated due to excessive control mortality.

### Table 1.

Facility: Motiva Enterprises LLC- PE NPDES # FL0000582 Facility Type: Bulk Petroleum storage Analysts: Della Parker-Hanson Location: 909 SE 24th Street Contact/District: Davis/Southeast terminal Joshua Ayres County: Broward Test type: static acute screen Gary Hardie Sample Collection Date: 03/25/02 Time: 1130 # tests: 1 Receiving Water: Intracoastal Waterway Marshall Faircloth Test Beginning Date 1 : 03/26/02 Time: 1600 Chlorination Type: non-chlorinated Class III Marine waters Test Ending Date 1 : 03/28/02 Time: 1400 Page 1 of 1 Reviewer: David Whiting 1 Data for C. dubia Organism: Ceriodaphnia dubia Life stage: <24 hours Conductivity Uncorrected Job number: TLH-2002-03-26-61 Sample/Diluent

SURVIVAL # Alive pH Temperature A Dissolved Oxygen B μmhos/cm sample number: 584365 Concentrations Volume(mL) 0 hr 24 hr 48 hr 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 48 hour Control A 0/20 5 5 5 8.2

- 8.2 24.7

- 23.4 8.1

- 7.8 170 215 Control B 0/20 5 5 4

- - 8.2

- - 23.4

- - 7.8

- 205 date: Control C 0/20 5 5 5

- - 8.3

- - 23.7

- - 7.8

- 220 by: Control D 0/20 5 5 5

- - 8.3

- - 23.3

- - 7.8

- 225 100% A 20/0 5 5 5 8.2

- 8.6 24.7

- 23.3 8.1

- 7.8 385 480 100% B 20/0 5 5 5

- - 8.6

- - 23.2

- - 7.8

- 460 Total Residual CL2 mg/L Method 100% C 20/0 5 5 5

- - 8.7

- - 23.1

- - 7.8

- 505 Field:

- - 100% D 20/0 5 5 5

- - 8.7

- - 23.1

- - 7.8

- 550 Lab: <0.03 Hach
A Temperatures of room and test incubator were continuously recorded on a strip chart recorder. Room Temperature range for the test period was 24.0-25.5 °C. Ammonia Total (mg/L) Unionized (mg/L) Incubator #3 temperature range for the test period was 23.5-25.5°C. Control water B The sample was aerated for 45 minutes to increase the dissolved oxygen (water flea): <0.017 <0.017 from 3.8 to 8.0 mg/L Control water (fish): <0.017 <0.017 100% Sample 3 : 0.09 0.009 Organism: 2 Cyprinella leedsi Life stage: 9 days Conductivity Uncorrected Alk & Hardness Alkalinity (mg/L) Hardness (mg/L) Sample/Diluent

SURVIVAL # Alive pH Temperature C Dissolved Oxygen B μmhos/cm Control water Concentrations Volume(mL) 0 hr 24 hr 48 hr 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 48 hour (water flea): 74 76 Control A 0/500 5 5 5 7.7 8.2 8.5 24.2 23.9 24.4 7.5 7.5 7.7 245 280 Control water (fish): 174 166 Control B 0/500 5 5 5 7.8 8.3 8.5 24.2 23.9 24.5 7.7 7.6 7.7 250 275 100% Sample: 170 164 Control C 0/500 5 5 4 7.8 8.3 8.5 24.2 23.9 24.4 7.8 7.5 7.8 255 280 Control D 0/500 5 5 5 7.8 8.3 8.5 24.2 24.0 24.2 7.8 7.6 7.8 255 275 100% A 500/0 5 5 5 8.3 8.5 8.5 24.5 24.0 24.4 7.9 7.6 7.6 385 425 100% B 500/0 5 5 5 8.3 8.5 8.5 24.7 24.0 24.6 7.9 7.5 7.6 385 420 100% C 500/0 5 5 5 8.3 8.4 8.5 24.8 24.0 24.5 8.0 7.5 7.5 400 420 100% D 500/0 5 4 4 8.3 8.4 8.5 24.9 24.0 24.4 8.0 7.4 7.5 395 445

2 The C. leedsi test set up on 3/26/02 was terminated due to excessive control mortality. C Temperatures of room and test incubator were continuously recorded on a strip chart recorder. The test was re-set on 3/28/02 at 1300 and ended 3/30/02 at 1135. Room Temperature range for the test period was 24.0-25.5 °C. 3 Ammonia was not detected in the sample on 3/28/02. Incubator #3 temperature range for the test period was 23.5-25.5°C.



### Data recorded during the 48-hour acute screening bioassays of a sample of effluent from the Motiva Enterprises LLC - Port Everglades North Terminal, 909 SE 24th Street, Fort Lauderdale, Broward County, Florida, NPDES# FL0000582, performed from 26 to 28 March 2002.

Data Transcription Verification 9/9/2002 Joshua Ayres Christa Pierini LIMS

# Table 2. Results of chemical analyses on the effluent from Motiva Enterprises LLC – Port Everglades North Terminal sampled on March 25, 2002.

Metals Aluminum_308 90 ug/L I 

# Cadmium 0.058 ug/L I 

# Calcium 57.8 mg/L A 

# Copper 2 ug/L I 

# Iron_271 940 ug/L A 

# Lead 2.85 ug/L A 

# Magnesium 3.5 mg/L A 

# Zinc 18.6 ug/L A



# Pesticides and Herbicides Ametryn 0.19 ug/L I 

# Atrazine 0.11 ug/L I



# Base, Neutral, & Acid Extractable Organics

None detected

Nutrients Ortho-phosphate 0.035 mg P/L Total phosphorous 0.068 mg P/L Ammonia-N 0.12 mg N/L Total Kjeldahl nitrogen 0.88 mg N/L

A 

# The reported value is an average of two or more values. I 

# The reported value is between the laboratory method detection limit and the laboratory practical

quantitation limit.Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type



### 1 N 2 5 3 F L 0 0 0 0 5 8 2 # # 0 2 0 3 2 5 # B # S # 2

#

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type



### 1 N 2 5 3 F L 0 0 0 0 5 8 2 # # 0 2 0 3 2 5 # X # S # 2

# Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks Remarks 

# The Bioassay of Motiva Enterprises LLC-Port Everglades North Terminal effluent sampled on March 25, 2002, NPDES # FL0000582.
Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)



# The Priority Pollutants Analysis for Bioassay of Motiva Enterprises LLC-Port Everglades North Terminal effluent sampled on March 25, 2002, NPDES # FL0000582.