Bioassays of 

Motiva Enterprises LLC – Port Everglades North Terminal 

Fort Lauderdale, Broward County, Florida 

NPDES #FL0000582 

Sampled 3/25/02 

November 2002 

Biology Section 

Division of Resource Assessment & Management 

Comprehensive Quality Assurance Plan #870346G 
Bioassays of 

Motiva Enterprises LLC – Port Everglades North Terminal 

Fort Lauderdale, Broward County, Florida 

NPDES #FL0000582 

 Biology Section 

 Bureau of Laboratories 

 November 2002 
Introduction 

Motiva Enterprises LCL – Port Everglades North Terminal, 909 SE 24th Street, Fort 

Lauderdale, Broward County, Florida, NPDES # 0000582, tests performed on 26 to 30 

March 2002. 

This bulk petroleum storage terminal discharges effluent once to several times a month, 

with a flow range of 0.0075 to 0.359 MGD. The wastewater is not treated prior to 

discharge, and usually consists of storm water runoff and brackish ground water from an 

onsite pond. The wastewater is discharged into the Class III marine waters of the 

Intracoastal waterway (facility information provided by Stanley Ganthier, FDEP, West 

Palm Beach). 

The toxicity tests discussed in this report were performed in accordance with methods 

described by Weber, 1993, Methods for Measuring the Acute Toxicity of Effluents and 

Receiving Waters to Freshwaters and Marine Organisms, 4th Edition, EPA/600/4-

90/027F. 

Results and Discussion 

The FDEP Biology Section performed two 48-hour static acute toxicity bioassays on a 

sample of effluent collected from the outfall of this facility. The test species used were 

the water flea, Ceriodaphnia dubia, and the bannerfin shiner, Cyprinella leedsi (Table 1). 

The sample was aerated for 45 minutes to increase the dissolved oxygen concentration 

from 3.8 to 8.0 mg/L. The C. leedsi test was terminated on March 27 after 24 hours due 

to excessive control mortality. The test was invalid due to 15% mortality of controls, 

while only 10% mortality is allowed in a valid test. This test was re-set on March 28 with 

the remaining bioassay sample. In the re-set bioassay, the 5% C. leedsi mortality in the 

100% effluent was equivalent to the 5% mortality in the C. leedsi controls; therefore the 

sample mortality can’t be attributed to toxicity of the sample (Table 1). 

Total residual chlorine was not detected in the bioassay sample. Total ammonia and 

unionized ammonia were detected in the original bioassay sample at concentrations of 

0.09 mg/L and 0.009 mg/L respectively. Total ammonia in the sample submitted for 

chemical analysis was 0.12 mg/L. When the C. leedsi test was re-set on March 28, 

ammonia was measured a second time and was not detected in the sample. Additional 

samples of effluent were collected from this facility and analyzed for herbicides, 

nutrients, metals, organic compounds, and pesticides. Several metals, two herbicides, and 

nutrients were detected (Table 2). 

Conclusion 

The sample of effluent collected on March 25, 2002 from this facility did not show acute 

toxicity to C. dubia test organisms within 48 hours. The C. leedsi test was invalidated due 

to excessive control mortality. 
Table 1.

Facility: Motiva Enterprises LLC- PE NPDES # FL0000582 Facility Type: Bulk Petroleum storage Analysts: Della Parker-Hanson

Location: 909 SE 24th Street Contact/District: Davis/Southeast terminal Joshua Ayres

County: Broward Test type: static acute screen Gary Hardie

Sample Collection Date: 03/25/02 Time: 1130 # tests: 1 Receiving Water: Intracoastal Waterway Marshall Faircloth

 Test Beginning Date1: 03/26/02 Time: 1600 Chlorination Type: non-chlorinated Class III Marine waters

Test Ending Date1: 03/28/02 Time: 1400 Page 1 of 1 Reviewer: David Whiting

1 Data for C. dubia

Organism: Ceriodaphnia dubia Life stage: <24 hours Conductivity

Uncorrected Job number: TLH-2002-03-26-61

Sample/Diluent SURVIVAL # Alive pH TemperatureA Dissolved OxygenBμmhos/cm sample number: 584365

Concentrations Volume(mL) 0 hr 24 hr 48 hr 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 48 hour

Control A 0/20 5 5 5 8.2 - 8.2 24.7 - 23.4 8.1 - 7.8 170 215

Control B 0/20 5 5 4 - - 8.2 - - 23.4 - - 7.8 - 205 date:

Control C 0/20 5 5 5 - - 8.3 - - 23.7 - - 7.8 - 220 by:

Control D 0/20 5 5 5 - - 8.3 - - 23.3 - - 7.8 - 225

100% A 20/0 5 5 5 8.2 - 8.6 24.7 - 23.3 8.1 - 7.8 385 480

100% B 20/0 5 5 5 - - 8.6 - - 23.2 - - 7.8 - 460 Total Residual CL2 mg/L Method

100% C 20/0 5 5 5 - - 8.7 - - 23.1 - - 7.8 - 505 Field: - -

100% D 20/0 5 5 5 - - 8.7 - - 23.1 - - 7.8 - 550 Lab: <0.03 Hach

A

 Temperatures of room and test incubator were continuously recorded on a strip chart recorder.

 Room Temperature range for the test period was 24.0-25.5 °C. Ammonia Total (mg/L) Unionized (mg/L) 

 Incubator #3 temperature range for the test period was 23.5-25.5°C. Control water

B

 The sample was aerated for 45 minutes to increase the dissolved oxygen (water flea): <0.017 <0.017

from 3.8 to 8.0 mg/L Control water (fish): <0.017 <0.017

100% Sample3: 0.09 0.009

Organism: 2Cyprinella leedsi Life stage: 9 days Conductivity

Uncorrected Alk & Hardness Alkalinity (mg/L) Hardness (mg/L) 

Sample/Diluent SURVIVAL # Alive pH TemperatureC Dissolved OxygenB

μmhos/cm Control water

Concentrations Volume(mL) 0 hr 24 hr 48 hr 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 24 hour 48 hour 0 hour 48 hour (water flea): 74 76

Control A 0/500 5 5 5 7.7 8.2 8.5 24.2 23.9 24.4 7.5 7.5 7.7 245 280 Control water (fish): 174 166

Control B 0/500 5 5 5 7.8 8.3 8.5 24.2 23.9 24.5 7.7 7.6 7.7 250 275 100% Sample: 170 164

Control C 0/500 5 5 4 7.8 8.3 8.5 24.2 23.9 24.4 7.8 7.5 7.8 255 280

Control D 0/500 5 5 5 7.8 8.3 8.5 24.2 24.0 24.2 7.8 7.6 7.8 255 275

100% A 500/0 5 5 5 8.3 8.5 8.5 24.5 24.0 24.4 7.9 7.6 7.6 385 425

100% B 500/0 5 5 5 8.3 8.5 8.5 24.7 24.0 24.6 7.9 7.5 7.6 385 420

100% C 500/0 5 5 5 8.3 8.4 8.5 24.8 24.0 24.5 8.0 7.5 7.5 400 420

100% D 500/0 5 4 4 8.3 8.4 8.5 24.9 24.0 24.4 8.0 7.4 7.5 395 445

2

 The C. leedsi test set up on 3/26/02 was terminated due to excessive control mortality. C Temperatures of room and test incubator were continuously recorded on a strip chart recorder.

The test was re-set on 3/28/02 at 1300 and ended 3/30/02 at 1135. Room Temperature range for the test period was 24.0-25.5 °C.

3

Ammonia was not detected in the sample on 3/28/02. Incubator #3 temperature range for the test period was 23.5-25.5°C.

Data recorded during the 48-hour acute screening bioassays of a sample of effluent from the Motiva Enterprises LLC - Port Everglades North Terminal, 909 SE 

24th Street, Fort Lauderdale, Broward County, Florida, NPDES# FL0000582, performed from 26 to 28 March 2002.

Data Transcription Verification

9/9/2002

Joshua Ayres

Christa Pierini

LIMS
Table 2. Results of chemical analyses on the effluent from Motiva Enterprises LLC – Port 

Everglades North Terminal sampled on March 25, 2002. 

 Metals 

Aluminum_308 90 ug/L I 

Cadmium 0.058 ug/L I 

Calcium 57.8 mg/L A 

Copper 2 ug/L I 

Iron_271 940 ug/L A 

Lead 2.85 ug/L A 

Magnesium 3.5 mg/L A 

Zinc 18.6 ug/L A 

 

Pesticides and Herbicides 

 

Ametryn 0.19 ug/L I 

Atrazine 0.11 ug/L I 

Base, Neutral, & Acid Extractable Organics 

 

None detected 

Nutrients 

Ortho-phosphate 0.035 mg P/L 

Total phosphorous 0.068 mg P/L 

Ammonia-N 0.12 mg N/L 

Total Kjeldahl nitrogen 0.88 mg N/L 

A The reported value is an average of two or more values. 

I The reported value is between the laboratory method detection limit and the laboratory practical 

quantitation limit. 
Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 5 8 2 # # 0 2 0 3 2 5 # B # S # 2

#

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 5 8 2 # # 0 2 0 3 2 5 # X # S # 2

#

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Remarks

The Bioassay of Motiva Enterprises LLC-Port Everglades North 

Terminal effluent sampled on March 25, 2002, NPDES # 

FL0000582.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

The Priority Pollutants Analysis for Bioassay of Motiva 

Enterprises LLC-Port Everglades North Terminal effluent 

sampled on March 25, 2002, NPDES # FL0000582.