# Title

Revised Application for an Experimental Use Perm it for 1507x59122 Corn Blended with 1507 Corn

and 59122 Corn Blended with Non-Bt Corn

Data Requirement

40 CFR Part 172

Authors

Jamie Staley and Tracy Rood

Submission Date

December 10, 2009

Submitter

Tracy A. Rood

Pioneer Hi-Bred International, Inc.

2450 SE Oak Tree Court

Ankeny, IA 50021

Submitter Reference Number

PHI-2009-118

Volume 1

# Volume 1

Table of Contents

Page

Statement of Conf identiality Claim ............................................................................................. ............. 2

EPA Form 8570-17 (Application fo r Experimental Us e Permit)............................................................... 5

EPA Form 8570-4 (Confidential Statement of Formula).......................................................................... 6

EPA Form 8570-34 (Certification with Respect to Da ta Cita tion)............................................................ 7

EPA Form 8570-35 (Data Matrix for 150 7x59122 corn blended with 1507 corn ) ................................... 8

EPA Form 8570-35 (Data Matrix for 59122 corn blended with non-Bt corn)......................................... 61

Section A. Produ ct Chemistry.................................................................................................. ............. 90

Section B. Pr oposed Labels .................................................................................................... ............. 91

Section C. To xicity Data ...................................................................................................... ................. 99

Section D. Residue Data ....................................................................................................... ............. 100

Section E. Effectiveness Data ................................................................................................. ........... 101

Section F. Tolerance if Testing Involves Use in Food or Feed........................................................... 102

Section G. Proposed Experimental Program...................................................................................... 103

G.1. Acreage Justification .................................................................................................... ........ 103

G.2. Participants in t he Experimental Program............................................................................ 105

G.3. Acreage and Seed Quantit ies by State for t he EUP Prog ram.............................................. 105

G.4. Details of the Proposed EUP Progra m ................................................................................ 111

G.5. Universal Pr otocol Procedures............................................................................................ . 111

Grower Evaluat ion Prot ocol ..................................................................................................... ..... 113

Research Tria l Prot ocol........................................................................................................ ......... 114

Confidential Attachment........................................................................................................ ............... 115

# List of Tables and Figures

Tables Page

Table 1. Proposed Acreage by State............................................................................................ ...... 106

Table 2. Proposed Ac reage by Protocol......................................................................................... .... 107

Table 3. Acreage by State for Grower Evaluati on Protoc ol................................................................ 108

Table 4. Acreage by State fo r Research Tria l Protoc ol ...................................................................... 109

Table 5. Amount of Seed (kg) and Active Ingredient (grams of Cry1F, Cry34Ab1 and

Cry35Ab1) by State ............................................................................................................. ................ 110

# EPA Forms 8570-4

Confidential Statement of Formula

{See CBI Cross Reference Number 1}

# EPA Form 8570-35

Data Matrix for 1507x59122 corn blended with 1507 cornForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage1of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAcute oral toxicity in mice: Cry1FBacillusthuringiensissubsp. aizawai delta endotoxin44691101 68467 PEREffectiveness data forBacillus thuringiensisvar.aizawai Cry1F insect control protein as expressed inmaize 4469110268467PERBackground document on resistance management 4469110368467PERProduct characterization data forBacillusthuringiensisvar. aizawai: Cry1F control protein asexpressed in maize4471480168467PERCharacterization of gene inserts –Bacillusthuringiensisaizawai: Cry1F control protein asexpressed in maize4471480268467PEREquivalency of microbial and maize expressedCry1F protein; characterization of test substancesfor biochemical and toxicological studies. In vitrodigestibility of microbialand maize expressed Cry1Fprotein under simulated gastric conditions44714803 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage2of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteQuantitative ELISA analysis of Cry1F expressionlevels in maize MPS inbred and hybrid lines 1360,1365, 1366 and 1369 (interim report)4471480468467PERComparison of the amino acid sequence similarity ofCry1F and PATproteins to KnPER allergen protein44971701 68467 PERMicrobialB.t.Cry1F (truncated) delta-endotoxin:Maize-insect-pest susceptibility study4502010168467PERCharacterization of inserted genes in Cry1F maizeline 15074502010268467PERCharacterization of expressed Cry1F protein inmaize tissues (pollen, grain, grain-containing feedand purified maize-expressed Cry1F protein) andmicrobial expressed Cry1F delta-endotoxin bybiological and biochemicalprocedures4502010368467PERQuantitative ELISA analysis of Cry1F and PATexpression levels in and compositional analysis ofmaize inbred and hybrid line 1362 and 150745020104 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage3of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEnvironmental fate of Cry1F protein incorporatedinto soil4502010568467PERCry1FBacillus thuringiensisvar. aizawai deltaendotoxin: an acute toxicity study with theearthworm in an artificial soil substrate4502010668467PERChronic exposure of Folsomia candida to bacteriallyexpressed Cry1F protein45020107 68467 PEROECD 207B.t.Cry1F delta endotoxin: A 48-hour static-renewalacute toxicity test with the Cladoceran (Daphniamagna) using bacterially expressedB.t.Cry1F deltaendotoxin, and pollen from maize expressingB.t.Cry1F delta endotoxin4502010868467PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary study with green lacewinglarvae 4502010968467PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary study with the ladybird beetle 45020110 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage4of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary toxicity study with parasitichymenoptera45020111 68467 PER71-2Transgenic corn expressingBacillus thuringiensisvar. aizawai (B.t.) Cry1F delta endotoxin: A dietarytoxicity study with Northern bobwhite45020112 68467 PERField surveys of beneficial arthropods associatedwithBacillus thuringiensisCry1F maize4502011368467PEREfficacy of Cry1F events TC1360 and TC15074502011468467PERCry1F binding studies4502011568467PERResistance management plan for transgenic maizeexpressing the Cry1F insecticidal protein fromBacillus thuringiensisvar. aizawai45020116 68467 PERSupplement to MRID 44714801: Supplemental data- Product characterization data forBacillusthuringiensisvar. aizawai: Cry1F control protein asexpressed in maize4502011768467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage5of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSupplement to MRID 44691101: Supplemental data- Acute oral toxicity in mice:Bacillus thuringiensisvar. aizawai: Cry1F delta endotoxin45020118 68467 PERPhosphinothricin acetyltransferase (PAT) protein: Invitro digestibility study45041501 68467 PERNon-target exposure and risk assessment forenvironmental dispersal of Cry1F maize pollen4504150268467PEREvaluation of the dietary effect(s) on honeybeedevelopment using bacterially expressedB.t.Cry1Fdelta endotoxin and pollen from maize expressingB.t.Cry1F delta endotoxin4504150368467PERWaiver request: Fish toxicity test with transgenicmaize (corn) containingBacillus thuringiensisvar.aiaqoi (B.t.) Cry1F delta endotoxin4504420168467PERHigh dose demonstration of Cry1F events TC1360and TC1507: European corn borer4513110168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage6of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteToxicity of the Cry1F protein to neonate larvae of themonarch buttefly (Danaus plexippus (Linneaus))45131102 68467 PERPublic interest document for Cry1F-protected corn 4513110329964OWNThermolability of Cry1F (truncated) delta endotoxin 4527480168467PERCompositional analysis of maize MPS hybrid line1507 4527480268467PERCry1F lateral flow test kit procedure for analyzingCry1F corn grain4527930168467PERMethod validation report for the determination ofCry1F delta endotoxin protein in grain by EnzymeLinked Immunosorbent Assay45279302 68467 PERPublic interest document for Cry1F protected corn 4529810129964OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage7of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSupplement to MRID 45131102: Supplemental data- High dose demonstration of Cry1F events TC1360and TC1507: European corn borer4530770168467PERWaiver request: Fish toxicity test to assess thepotential effects of maize containingBacillusthuringiensisvar. aizawai (Bt) Cry1F insecticidalprotein (ICP) in native fish4530770268467PERSupplement to MRID 45020109: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with green lacewing larvae4530780168467PERSupplement to MRID 45020110: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with green ladybird beetle45307802 68467 PERSupplement to MRID 45020111: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with parasitic hymenoptera4530780368467PERSupplement to MRID 45020106: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: An acutetoxicity study with the earthworm in an artificial soilsubstrate 4530780468467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage8of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSupplement to MRID 45041503: Evaluation of thedietary effect(s) on honeybee development usingbacterially expressedB.t.Cry1F delta endotoxin andpollen from maize expressingB.t.Cry1F deltaendotoxin45307805 68467 PERSupplement to MRID 45298101: Supplemental data- Public interest document for Cry1F protected corn45309401 29964 OWNExposure and risk assessment of Herculex I Bt fieldcorn pollen to Karner blue butterfly45512901 68467 PERNutritional equivalency ofB.t.Cry1F maize - poultryfeeding study45622001 68467 PERField survey of beneficial arthropods associated withBacillus thuringiensisCry1F maize4564800168467PERField surveys of non-target invertebrate populationsinB.t.corn45652001 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage9of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteDevelopment and characterization of EnzymeLinked Immunosorbent Assay (ELISA) for detectionof Cry1F protein45685601 68467 PERIndependent laboratory validation of method GRM02.13, determination of Cry1F delta endotoxinprotein in corn grain by an of Enzyme-LinkedImmunosorbent Assay4568560268467PERSupplement to MRID 45131102: Toxicity of theCry1F protein to neonate larvae of the monarchbutterfly (Danaus plexippus (Linneaus))45759701 68467 PERStewardship of Herculex I Insect Protection withrespect to the secondary lepidopteran pest, westernbean cutworm (Richia albicostaSmith)45896601 29964 OWNLack of cross reactivity between Cry1F protein inHerculex I maize and the dust mite Der p7 proteinwith human sera positive for Der p7-IgE4644400168467PERMonitoring the susceptibility of European corn borerto Cry1Ab and Cry1F Bt proteins: Results from the2004 collections and diapausing larvae collected in2003 4658310180778PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage10of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteStewardship of event TC1507 maize with respect tothe secondary lepidopteran pests lesser corn stalkborer (Elasmopalpus lignosellusZeller), southerncorn stalk borer (Diatraea crambidiodesGrote), andsugarcane borer (Biatraea saccharalisFabricius)46600201 68467 PERSlide presentation summarizing European cornborer and Cry1F resistance monitoring update4669580168467PERInsect resistance management complianceassurance program report for corn borer-protectedBt corn4674780180778PERField surveys of non-target invertebrate populationsin Bt corn: Supplement to MRID No. 456520014678460180778PERMonitoring the susceptibility of corn lepidopteranpests to Cry1Ab and Cry1F proteins: 2005monitoring results4687490180778PERResearch results on 2004 European corn borercollections from Hamilton County, Iowa: Cry1F4701120168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage11of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteInsect resistance management complianceassurance program report for corn borer-protectedBt corn, corn rootworm-protected Bt corn, and cornborer/corn rootworm protected stacked Bt corn4704440180778PERMonitoring the susceptibility of corn lepidopteranpests to Cry1Ab and Cry1F proteins: 2006monitoring results47118401 80778 PERSoil accumulation of Cry1F protein after three yearsof cropping with Herculex I corn4712070168467PERTC1507 maize and fall armyworm in Puerto Rico 4717600168467PERProduct characterization data forBacillusthuringiensisPS149B1 13.6 kDa and 43.8 kDainsecticidal crystal proteins expressed in transgenicmaize plants4524220168467PEREquivalency of microbial and maize-expressedPS149B1 proteins45242203 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage12of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study45242204 68467 PERComparison of the amino acid sequence of theBacillus thuringiensisstrain PS149B1 13.6 kDa and43.8 kDa insecticidal crystal proteins to KnPERprotein allergens4524220568467PERCharacterization ofPseudomonasproduced andtransgenic maize expressed phosphinothricinacetyltransferase (PAT) protein4524220668467PERPS149B1 14 kDa protein: Acute oral toxicity study inCD-1 mice 45242207 68467 PERPS149B1 44 kDa protein: Acute oral toxicity study inCD-1 mice45242208 68467 PERPS149B1 14 kDa and 44 kDa proteins: Acute oraltoxicity study in CD-1 mice45242209 68467 PERPS149B1 binary insecticidal crystal protein: A dietarytoxicity study with ladybird beetle45242210 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage13of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteThe tri-trophic interaction between PS149B1transformed maize, corn leaf aphid and ladybirdbeetle 4524221168467PERIn vitro digestibility ofPS149B1 proteins4524221268467PERQuantitative ELISA analysis of PS149B1 proteinexpression levels in hybridand inbred lines of maizeevent TC5639 (interim report)4524221368467PERDegradation of microbial binary PS149B1 deltaendotoxin in a representative soil from the midWestern USA maize growing region45242214 68467 PERProduct durability plan for transgenic maizeexpressing insecticidal crystal protein fromBacillusthuringiensisstrain PS149B1 during theexperimental use period4524221568467PERField efficacy of PS149B1 maize events against cornrootworms 45242216 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage14of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteMicrobial PS149B1 binary insecticidal crystalproteins, pollen expressing PS149B1 binaryinsecticidal crystal proteins, and individual PS149B114 kDa and 44 kDa insecticidal crystal proteins4534070168467PERPS149B1binary insecticidal crystal protein: Acutetoxicity study to the earthworm in an artificialsubstrate45360201 68464 PERLateral flow test kit method validation for thedetection of the PS149B1 14 kDa and 44 kDaprotein in maize grain45383401 68467 PERHeat lability of individual proteins of the PS149B1binary ICP 45584501 68467 PERIn vitro simulated gastric fluid digestibility study ofmicrobially derived Cry34Ab1 protein45584502 68467 PERCharacterization of Cry34Ab1 and Cry35Ab1 fromrecombinantPseudomonas flourescensandtransgenic maize45790401 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage15of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteCharacterization of DNA inserted into transgeniccorn events (Cry34Ab1 and Cry35Ab1)4579040268467PERPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout,OncorhynchusmykissWallbaum4579040368467PERPS149B1 binary insecticidal crystal protein: Acutetoxicity study with Daphnid,Daphniamagna Straus 4579040468467PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to parasitic hymenoptera (Nasoniavitripennis)45790405 68467 PERAssessment of the chronic toxicity of diet containingBacillus thuringiensisPS149B1 insecticidal crystalprotein to collembolan (Folsomia candida) 45790406 68467 PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to green lacewing (Chrysoperla carnea)45790407 68467 PERSDS-PAGE sensitivity analysis for Cry35Ab1 insupport of the simulated gastric fluid digestibilitystudy MRID 452422124579040868467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage16of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteTrait durability and experimental use of transgenicmaize expressing the insecticidal crystalline proteinsCry34Ab1and Cry35Ab145790509 68467 PERField efficacy of Cry34Ab1/Cry35Ab1 maize eventsagainst corn rootworms45790410 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17658)45790501 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662) 4579060168467PERSummary heat lability studies withCry34Ab1/Cry35Ab1 4580860168467PERQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP176584583310168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage17of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP1766245833102 68467 PERSlide presentation summarizingCry34Ab1/Cry35Ab1 heat inactivation studies4586020168467PERProbe MOA studies to assess potential for proteinsynthesis inhibition byBacillus thuringiensisPS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbitreticulocyte assay: Re-examination of lab notebookdata 4594280168467PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662) 4603000168467PERPublic interest document for Cry34/35Ab1 cornrootworm-protected corn4612270129964OWNIndependent laboratory validation Pioneer Hi-BredInternational, Inc. ELISA method for thequantification of Cry34Ab1 protein from transgenicplants 4612390168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage18of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteIndependent laboratory validation of DowAgroSciences method GRM 03.13, “ Determinationof Cry35Ab1 insecticidal crystal protein in maizetissue by Enzyme-Linked Immunosorbent Assay” 4612390268467PERCry34/35 protein distribution and familiarity4612390368467PERAgronomic characteristics, quantitative ELISA andnutrient composition analysis of hybrid maize linescontaining cry34Ab1, cry35Ab1 and pat genes: Chilelocations 4612390468467PERBiological equivalency of Cry34/35Ab1 insecticidalcrystal protein in transgenic plants derived fromtransgenicPseudomonas flourscens46123905 68467 PERCharacterization of Cry34Ab and Cry35Ab1 proteinsderived from transgenic maize event E4497.59.1.22(DAS-59122-7)46123906 68467 PERCharacterization of phosphinothricinacetyltransferase (PAT) derived from transgenicmaize event E4497.59.1.2246123907 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage19of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteCharacterization of DNA inserted into transgeniccorn events DAS-45216-6 and DAS-59122-74612390868467PERDetailed characterization of DNA into transgeniccorn events DAS-45216-6 and DAS-59122-74612390968467PEREvaluation of microbederived Cry34Ab1 andCry35Ab1 proteins for protein synthesis inhibitionactivity 4612391068467PERNutritional equivalency study of maize containingCry34Ab1 and Cry35Ab1: Poultry feeding study4612391168467PERThe effect of Cry34Ab1/Cry35Ab1 proteins on thedevelopment and mortality of the ladybird beetleColeomegilla maculataDeGeer4612391268467PERNon-target invertebrate ecological risk assessmentfor field corn expressing Cry34Ab1 and Cry35Ab1insecticidal crystal proteins in event DAS-59122-746123913 68467 PEREvaluation of the impact corn rootworm controlstrategies on non-target arthropods46123914 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage20of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteInvestigations into dose of Cry34Ab1/Cry35Ab1rootworm-resistant maize event DAS-59122-7against western and northern corn rootworms insupport of trait durability plans4612391568467PEREffect on western corn rootworm adults feeding onCry34/35Ab1 corn rootworm-protected corn tissueand implication for product durability46123916 68467 PEREvaluation of endangered/threatened insect speciesrelative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids4612391768467PERTrait durability plan for Cry34/35 corn rootwormprotected corn event DAS-59122-7 followingcommercialization46123918 68467 PERSimulations of corn rootworm adaptation to Cry34/35corn rootworm protected corn in support of traitdurability plans for event DAS-59122-74612391968467PERDigestion of allergenic and non-allergenic proteins insimulated gastric fluid46123920 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage21of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteDigestion efficiency of allergens and non-allergensin simulated gastric fluid:Bacillus thuringiensisCry34/35Ab1 construct PHP1766246388601 68467 PERLack of Cry34Ab1/Cry35Ab1 co-association insolution46556801 68467 PEREvaluation of the sequencesimilarities of theCry34Ab1, Cry35Ab1 and PAT proteins to the publicprotein sequence datasets4658470168467PERThermolability of PS149B1 binary delta endotoxin 4535840168467PERSummary information in support of the Section 3registration of Herculex XTRA Insect Protection corn46297101 68467 PERCharacterization of a stacked hybrid of transgeniccorn lines DAS-01507-1 and DAS-59122-7 bycomparison to the individual lines46297102 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage22of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAgronomic characteristics, quantitative ELISA andnutrient composition analysis of hybrid maize linescontaining cry1F, cry34Ab1, cry35Ab1 and patgenes: US and Canada locations4629710368467PERAmended report:: Agronomic characteristics,quantitative ELISA and nutrient composition analysisof hybrid maize lines containing cry1F, cry34Ab1,cry35Ab1 and pat genes: US and Canada locations 4634380168467PERNutritional equivalency study of stacked hybrid oftransgenic maize line 1507 (event DAS-01507-1)and 59122 (event DAS-59122-7): Poultry feedingstudy 4634380368467PERComparison of insect efficacy and expression inCry34/35 corn events DAS-59122-7 and DAS59132-8 expressed as a single and a stack withDAS-01507-1 4634380468467PERField efficacy of Herculex XTRA (DAS-01507-1xDAS-59122-7) insect resistant corn4634380568467PERInvestigation of potential interaction between Cry1Fand the binary Cry34Ab1/Cry35Ab1 proteins4634380668467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage23of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteThe effect of maize pollen expressing the Cry1F andCry34Ab1/Cry35Ab1 proteins on the developmentand mortality of the ladybird beetleColeomegillamaculataDeGeer4634380768467PERLaboratory bioassay assessment of corn pollenprotein (Cry34/35 and Cry1F, stacked transgenes),and their effect on the larvae of monarch butterfly 4634380868467PEREquimolar ratio of Cry1F (tr) + Cry35Ab1+Cry34Ab1: Acute oral toxicity study in CD-1 mice 4638430168467PERProposed revisions to IRM-related registrationrequirements for Cry1 Plant IncorporatedProtectants in field corn4740700180778PERCharacterization of instar-specific response andlarval recovery of western corn rootworm exposed toDAS-59122-7 maize4735670129964OWNEffect of off-type seeds on the precision of sublethalcorn rootworm seedling assay results4735670229964OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage24of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAn observational study characterizing the relativequantity and distribution of Cry34Ab1 and Cry35Ab1insecticidal proteins in roots of maize containingevent DAS-59122-74735670329964OWNQuantifying the concentrations of Cry34Ab1 andCry35Ab1 in 59122 maize grown in the sublethalcorn rootworm seedling assay system4735670429964OWNField measures of adult cornrootworm (Coleoptera:Chrysomelidae) production when refuge is deployedas a seed blend with DAS-59122-7 maize47356705 29964 OWNImpact of western and northern corn rootwormexposure to DAS-59122-7 in a block planting and aseed blend on life history traits of adults47356706 29964 OWNInvestigating the effect of insecticidal proteinsCry34/35Ab1 on western corn rootworm hostsearching behavior47356707 29964 OWNModeling durability of DAS-59122-7 maize efficacyagainst corn rootworm (Coleoptera: Chrysomelidae)when refuge is deployed as a seed blend4735670829964OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage25of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteTrait durability plan for Optimum® AcreMaxTM1Insect Protection following commercialization47356709 29964 OWNBenefits document for Optimum® AcreMaxTM1Insect Protection47356710 29964 OWNLiterature references for Optimum® AcreMaxTM1Insect Protection47356711 29964 OWNAdditional responsesfor “Pioneer’s benefitsdocument in support of a Sec(3) product registrationfor Optimum® AcreMaxTM1 Insect Protection, alsoknown as stack Herculex XTRA 9Cry34/35Ab1 +Cry1F) with Herculex I (Cry1F refuge seed for CRW)[MRID 473567-10 and supplemental data]”4768380129964OWNInsecticidal seed treatments for corn rootworm:Inconsistent protection for refuge plants 47789402 29964 OWNAddendum to the trait durability plan for Optimum®AcreMaxTM1 Insect Protection followingcommercialization47789403 29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage26of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteLiterature referencessupporting the additionalstudies for Optimum® AcreMaxTM1 Insect Protection47789404 29964 OWNAddendum to the Benefits of Optimum®AcreMaxTM147795101 29964 OWNAddendum to: A spatially explicit computersimulation study of corn rootworm,Diabroticaspp.[Coleoptera: Chrysomelidae]4787100129964OWNLiterature references cited in the modeling reportPHI-2009-125 4787100229964OWNAdditional addendum to MRID 473567-08: Aspatially explicit computer simulation study of cornrootworm,Diabroticaspp.[Coleoptera:Chrysomelidae] 4788790129964OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage1of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Public File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage2of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage3of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage4of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage5of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage6of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER29964 OWN68467 PER68467 PER68467 PER68467 PER29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage7of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage8of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER29964 OWN68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage9of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER29964 OWN68467 PER80778 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage10of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER80778 PER80778 PER80778 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage11of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note80778 PER80778 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage12of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage13of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage14of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:2996-EUP-Page15of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage16of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage17of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER29964 OWN68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage18of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage19of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage20of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage21of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage22of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage23of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER80778 PER29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage24of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage25of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage26of26Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122 corn blended with 1507 cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File Copy

# EPA Form 8570-35

Data Matrix for 59122 corn blended with non-Bt cornForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage1of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisPS149B1 13.6 kDa and 43.8 kDainsecticidal crystal proteins expressed in transgenicmaize plants4524220168467PEREquivalency of microbial and maize-expressedPS149B1 proteins45242203 68467 PERMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study45242204 68467 PERComparison of the amino acid sequence of theBacillus thuringiensisstrain PS149B1 13.6 kDa and43.8 kDa insecticidal crystal proteins to KnPERprotein allergens4524220568467PERCharacterization ofPseudomonasproduced andtransgenic maize expressed phosphinothricinacetyltransferase (PAT) protein4524220668467PERPS149B1 14 kDa protein: Acute oral toxicity study inCD-1 mice4524220768467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage2of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NotePS149B1 44 kDa protein: Acute oral toxicity study inCD-1 mice45242208 68467 PERPS149B1 14 kDa and 44 kDa proteins: Acute oraltoxicity study in CD-1 mice45242209 68467 PERPS149B1 binary insecticidal crystal protein: A dietarytoxicity study with ladybird beetle 45242210 68467 PERThe tri-trophic interaction between PS149B1transformed maize, corn leaf aphid and ladybirdbeetle 4524221168467PERIn vitro digestibility ofPS149B1 proteins4524221268467PERQuantitative ELISA analysis of PS149B1 proteinexpression levels in hybridand inbred lines of maizeevent TC5639 (interim report)45242213 68467 PERDegradation of microbial binary PS149B1 deltaendotoxin in a representative soil from the midWestern USA maize growing region45242214 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage3of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct durability plan for transgenic maizeexpressing insecticidal crystal protein fromBacillusthuringiensisstrain PS149B1 during theexperimental use period4524221568467PERField efficacy of PS149B1 maize events against cornrootworms 45242216 68467 PERMicrobial PS149B1 binary insecticidal crystalproteins, pollen expressing PS149B1 binaryinsecticidal crystal proteins, and individual PS149B114 kDa and 44 kDa insecticidal crystal proteins4534070168467PERPS149B1binary insecticidal crystal protein: Acutetoxicity study to the earthworm in an artificialsubstrate45360201 68464 PERLateral flow test kit method validation for thedetection of the PS149B1 14 kDa and 44 kDaprotein in maize grain4538340168467PERHeat lability of individual proteins of the PS149B1binary ICP 45584501 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage4of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteIn vitro simulated gastric fluid digestibility study ofmicrobially derived Cry34Ab1 protein 45584502 68467 PERCharacterization of Cry34Ab1 and Cry35Ab1 fromrecombinantPseudomonas flourescensandtransgenic maize45790401 68467 PERCharacterization of DNA inserted into transgeniccorn events (Cry34Ab1 and Cry35Ab1)45790402 68467 PERPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout,OncorhynchusmykissWallbaum4579040368467PERPS149B1 binary insecticidal crystal protein: Acutetoxicity study with Daphnid,Daphniamagna Straus45790404 68467 PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to parasitic hymenoptera (Nasoniavitripennis)4579040568467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage5of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAssessment of the chronic toxicity of diet containingBacillus thuringiensisPS149B1 insecticidal crystalprotein to collembolan (Folsomia candida) 45790406 68467 PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to green lacewing (Chrysoperla carnea) 45790407 68467 PERSDS-PAGE sensitivity analysis for Cry35Ab1 insupport of the simulated gastric fluid digestibilitystudy MRID 452422124579040868467PERTrait durability and experimental use of transgenicmaize expressing the insecticidal crystalline proteinsCry34Ab1and Cry35Ab145790509 68467 PERField efficacy of Cry34Ab1/Cry35Ab1 maize eventsagainst corn rootworms45790410 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17658) 4579050168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage6of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662)45790601 68467 PERSummary heat lability studies withCry34Ab1/Cry35Ab145808601 68467 PERQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP1765845833101 68467 PERQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP176624583310268467PERSlide presentation summarizingCry34Ab1/Cry35Ab1 heat inactivation studies4586020168467PERProbe MOA studies to assess potential for proteinsynthesis inhibition byBacillus thuringiensisPS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbitreticulocyte assay: Re-examination of lab notebookdata 4594280168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage7of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662)46030001 68467 PERPublic interest document for Cry34/35Ab1 cornrootworm-protected corn4612270129964OWNIndependent laboratory validation Pioneer Hi-BredInternational, Inc. ELISA method for thequantification of Cry34Ab1 protein from transgenicplants 4612390168467PERIndependent laboratory validation of DowAgroSciences method GRM 03.13, “ Determinationof Cry35Ab1 insecticidal crystal protein in maizetissue by Enzyme-Linked Immunosorbent Assay” 4612390268467PERCry34/35 protein distribution and familiarity4612390368467PERAgronomic characteristics, quantitative ELISA andnutrient composition analysis of hybrid maize linescontaining cry34Ab1, cry35Ab1 and pat genes: Chilelocations 4612390468467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage8of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteBiological equivalency of Cry34/35Ab1 insecticidalcrystal protein in transgenic plants derived fromtransgenicPseudomonas flourscens46123905 68467 PERCharacterization of Cry34Ab and Cry35Ab1 proteinsderived from transgenic maize event E4497.59.1.22(DAS-59122-7) 4612390668467PERCharacterization of phosphinothricinacetyltransferase (PAT) derived from transgenicmaize event E4497.59.1.2246123907 68467 PERCharacterization of DNA inserted into transgeniccorn events DAS-45216-6 and DAS-59122-746123908 68467 PERDetailed characterization of DNA into transgeniccorn events DAS-45216-6 and DAS-59122-746123909 68467 PEREvaluation of microbederived Cry34Ab1 andCry35Ab1 proteins for protein synthesis inhibitionactivity46123910 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage9of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteNutritional equivalency study of maize containingCry34Ab1 and Cry35Ab1: Poultry feeding study4612391168467PERThe effect of Cry34Ab1/Cry35Ab1 proteins on thedevelopment and mortality of the ladybird beetleColeomegilla maculataDeGeer4612391268467PERNon-target invertebrate ecological risk assessmentfor field corn expressing Cry34Ab1 and Cry35Ab1insecticidal crystal proteinsin event DAS-59122-7 4612391368467PEREvaluation of the impact corn rootworm controlstrategies on non-target arthropods4612391468467PERInvestigations into dose of Cry34Ab1/Cry35Ab1rootworm-resistant maize event DAS-59122-7against western and northern corn rootworms insupport of trait durability plans4612391568467PEREffect on western corn rootworm adults feeding onCry34/35Ab1 corn rootworm-protected corn tissueand implication for product durability46123916 68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage10of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of endangered/threatened insect speciesrelative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids4612391768467PERTrait durability plan for Cry34/35 corn rootwormprotected corn event DAS-59122-7 followingcommercialization 4612391868467PERSimulations of corn rootworm adaptation to Cry34/35corn rootworm protected corn in support of traitdurability plans for event DAS-59122-746123919 68467 PERDigestion of allergenic and non-allergenic proteins insimulated gastric fluid4612392068467PERDigestion efficiency of allergens and non-allergensin simulated gastric fluid:Bacillus thuringiensisCry34/35Ab1 construct PHP1766246388601 68467 PERLack of Cry34Ab1/Cry35Ab1 co-association insolution 4655680168467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage11of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the sequencesimilarities of theCry34Ab1, Cry35Ab1 and PAT proteins to the publicprotein sequence datasets4658470168467PERThermolability of PS149B1 binary delta endotoxin 4535840168467PERProposed revisions to IRM-related registrationrequirements for Cry1 Plant IncorporatedProtectants in field corn47407001 80778 PERCharacterization of instar-specific response andlarval recovery of western corn rootworm exposed toDAS-59122-7 maize47356701 29964 OWNEffect of off-type seeds on the precision of sublethalcorn rootworm seedling assay results47356702 29964 OWNAn observational study characterizing the relativequantity and distribution of Cry34Ab1 and Cry35Ab1insecticidal proteins in roots of maize containingevent DAS-59122-74735670329964OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage12of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteQuantifying the concentrations of Cry34Ab1 andCry35Ab1 in 59122 maize grown in the sublethalcorn rootworm seedling assay system4735670429964OWNField measures of adult cornrootworm (Coleoptera:Chrysomelidae) production when refuge is deployedas a seed blend with DAS-59122-7 maize47356705 29964 OWNImpact of western and northern corn rootwormexposure to DAS-59122-7 in a block planting and aseed blend on life history traits of adults47356706 29964 OWNInvestigating the effect of insecticidal proteinsCry34/35Ab1 on western corn rootworm hostsearching behavior47356707 29964 OWNModeling durability of DAS-59122-7 maize efficacyagainst corn rootworm (Coleoptera: Chrysomelidae)when refuge is deployed as a seed blend4735670829964OWNTrait durability plan for Optimum® AcreMaxTM1Insect Protection following commercialization47356709 29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage13of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteBenefits document for Optimum® AcreMaxTM1Insect Protection4735671029964OWNLiterature references for Optimum® AcreMaxTM1Insect Protection47356711 29964 OWNAdditional responsesfor “Pioneer’s benefitsdocument in support of a Sec(3) product registrationfor Optimum® AcreMaxTM1 Insect Protection, alsoknown as stack Herculex XTRA 9Cry34/35Ab1 +Cry1F) with Herculex I (Cry1F refuge seed for CRW)[MRID 473567-10 and supplemental data]”4768380129964OWNInsecticidal seed treatments for corn rootworm:Inconsistent protection for refuge plants 47789402 29964 OWNAddendum to the trait durability plan for Optimum®AcreMaxTM1 Insect Protection followingcommercialization47789403 29964 OWNLiterature referencessupporting the additionalstudies for Optimum® AcreMaxTM1 Insect Protection47789404 29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage14of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAddendum to the Benefits of Optimum®AcreMaxTM1 4779510129964OWNAddendum to: A spatially explicit computersimulation study of corn rootworm,Diabroticaspp.[Coleoptera: Chrysomelidae]47871001 29964 OWNLiterature references cited in the modeling reportPHI-2009-12547871002 29964 OWNAdditional addendum to MRID 473567-08: Aspatially explicit computer simulation study of cornrootworm,Diabroticaspp.[Coleoptera:Chrysomelidae] 4788790129964OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage1of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Public File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage2of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage3of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage4of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage5of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage6of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage7of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER29964 OWN68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage8of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage9of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467PER68467PER68467PER68467PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage10of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage11of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER80778 PER29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage12of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage13of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:December 10, 2009EPA Reg. No. / File Symbol:29964-EUP-IPage14of14Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122 corn blended with non-Bt cornIngredientBacillus thuringiensisCry34/35Ab1 insecticidal proteinsand the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleTracy A. RoodSr. Registration ManagerDateDecember 10, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File Copy

# Section A. Product Chemistry

Pioneer is developing a new blended seed product that controls Lepidopteran and corn rootworm

pests and contains a built-in ( i.e. “in the bag”) corn rootworm refuge. The product consists of a high

percentage of 1507x59122 combined trait seed and a low percentage of refuge seed (1507 corn,

which does not contain a Bt trait that controls corn rootworm) blended together in a package of seed.

An additional product, 59122 corn blended with non-Bt corn is also being evaluated as a blended seed

product that controls corn rootworm pests and cont ains an “in the bag” corn rootworm refuge. The

blended 59122 product consists of a high percentage of 59122 corn blended with a low percentage of

non-Bt refuge corn in a package of seed.

# Section B: Proposed Experimental Use Permit Labels

The following pages contain the proposed Master Lab el for the EUP, including labels for the following

EUP Test Plants:

• 1507x59122 corn blended with 1507 corn (5% Blend)

• 1507x59122 corn blended with 1507 corn (10% Blend)

• 59122 corn blended with non-Bt corn (5% Blend)

• 59122 corn blended with non-Bt corn (10% Blend)

# Master Label

29964-EUP-I

Subparts:

1) 1507x59122 Corn Blended with 1507 Corn (5% Blend)

2) 1507x59122 Corn Blended with 1507 Corn (10% Blend)

3) 59122 Corn Blended with Non-Bt Corn (5% Blend)

4) 59122 Corn Blended with Non-Bt Corn (10% Blend)

# 1) 1507x59122 Corn Blended with 1507 Corn

(5% Blend)

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients of Component 1 (1507x59122 corn) : 95% of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS-Ø15Ø7-1......................................................................... ....................... <0.00038%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... ........................ <0.00633%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... ........................ <0.00034%*

Inert Ingredient:

Phosphinothricin acetyltransferase produced by the pat gene and the genetic material

necessary for its production in corn........................................................................................... ....................................... <0.00004%*

Active Ingredients of Component 2 (1507 corn) : 5 % of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS-Ø15Ø7-1......................................................................... ......................<0.01415%**

Inert Ingredient:

Phosphinothricin acetyltransferase ( PAT) protein and the genetic material

necessary for its production in corn........................................................................................... ........................................<0.0020%**

* % total protein on a dry wt. basis as expressed in grain

** % total extractable protein as expressed in grain



# CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: 515-270-4036

7300 NW 62 nd 

# Ave Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

# DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 2) 1507x59122 Corn Blended with 1507 Corn

(10% Blend)

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted



# .

Active Ingredients of Component 1 (1507x59122 corn) : 95% of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS-Ø15Ø7-1......................................................................... ....................... <0.00038%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... ........................ <0.00633%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... ........................ <0.00034%*

Inert Ingredient:

Phosphinothricin acetyltransferase produced by the pat gene and the genetic material

necessary for its production in corn........................................................................................... ....................................... <0.00004%*

Active Ingredients of Component 2 (1507 corn) : 5 % of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS-Ø15Ø7-1......................................................................... ......................<0.01415%**

Inert Ingredient:

Phosphinothricin acetyltransferase ( PAT) protein and the genetic material

necessary for its production in corn........................................................................................... ........................................<0.0020%**

* % total protein on a dry wt. basis as expressed in grain

** % total extractable protein as expressed in grain



# CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc Phone: 515-270-4036

7300 NW 62 nd 

# Ave. Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

# DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 3) 59122 Corn Blended with Non-Bt Corn

(5% Blend)

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain corn rootworm pests: Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... .......................... <0.0117%*

Bacillus thuringiensis Cry35Ab1protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... ...................... <0.000161%*

Inert Ingredient:

Phosphinothricin acetyltransferase ( PAT) protein and the genetic material

necessary for its production in corn........................................................................................... ................................... <0.0000069%*

* % total protein on a dry wt. basis as expressed in grain



# CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc Phone: 515-270-4036

7300 NW 62 nd 

# Ave. Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 4) 59122 Corn Blended with Non-Bt Corn

(10% Blend)

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain corn rootworm pests: Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... .......................... <0.0117%*

Bacillus thuringiensis Cry35Ab1protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122-7 ......................................................................... ...................... <0.000161%*

Inert Ingredient:

Phosphinothricin acetyltransferase ( PAT) protein and the genetic material

necessary for its production in corn........................................................................................... ................................... <0.0000069%*

* % total protein on a dry wt. basis as expressed in grain



# CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc Phone: 515-270-4036

7300 NW 62 nd 

# Ave. Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# Section C. Toxicity Data

The environmental, human and animal safety of Cry1 F and Cry34/35Ab1 insecticidal proteins has

been previously reviewed by EPA for events 15 07 and 59122 and concluded to have no adverse

effects (U.S. EPA, 2001 & 2005). These proteins we re granted an exemption from the requirement

of a tolerance in corn by EPA on June 6, 2001 (40CFR §174.520) and September 21, 2005 (40CFR

§174.506), respectively.

# Section D. Residue Data

Residue data are not applicable as permanent tolerance exemptions exist for Cry1F (40CFR

§174.520) and Cry34/35Ab1 proteins (40CFR §174.506).

# Section E. Effectiveness Data

Not required under 40CFR §158.202(i).

# Section F. Tolerance if Testing Involves Use in Food or Feed

Cry1F protein is exempt from the requirem ent of a tolerance in corn: 40CFR §174.520.

Cry34Ab1 and Cry35Ab1 proteins are exempt from th e requirement of a tolerance in corn: 40CFR

§174.506.

Phosphinothricin acetyltransferase (PAT is exempt fr om the requirement of a tolerance in all plants:

40CFR §174.522.

# Section G. Proposed Experimental Program

G1. Acreage Justification

Pioneer is requesting a total of 36,670 acres in 11 states for 2010 in order to continue research,

testing and evaluation of blended refuge concepts.

This acreage is justified because of the wide diversity of environments and conditions where

1507x59122 corn blended with 1507 corn and 59122 corn blended with non-Bt corn will be deployed.

Experiments will be grown across diverse growing env ironments with a cross- section of cooperators

and defined research protocols. The result w ill be data and observations necessary for product

characterization, evaluation and advancement and product stewardship. Specific justification includes

the following:

i. Placement of experiments with cooperators will not be finaliz ed until spring, since weather

conditions can significantly affect planting locations. Cool and wet conditions may force

cooperators to alter their pl anting intentions. Experiments may need to be moved within a

state or across state lines. Therefore, the proposed EUP acreage needs to have the

flexibility to allow for the possibility of experiments being shifted among locations and

states.

ii. Pioneer may be testing various insecticide seed treatments (ISTs), including a low/base

rate (i.e., non-rootworm rate) of Cruiser IST and high rates of Cruiser and/or Poncho ISTs.

iii. Pioneer will be testing approximately 10-15 different hybrid platforms in order to

understand how blends perform in different genetic backgrounds in varying field and

agronomic conditions.

iv. Pioneer will be testing efficacy of blended refuge concepts against different rootworm

species and unique rootworm biotypes:

• Diabrotica virgifera virgifera(western corn rootworm), including the organophosphateresistant biotypes in the western Corn Belt and the eastern variant in the eastern Corn

Belt,

• Diabrotica barberi (northern corn rootworm), including areas of extended egg

diapause; and

• Geographies with mixed northern and western corn rootworm populations.

v. Pioneer will be testing in up to 11 states. There are four major populations/biotypes of

corn rootworm that encompass ov erlapping geographic regions.

a. In the Western Region, western corn ro otworms have developed a biotype that is

resistant to organophosphate insecticides.

b. In the Eastern Region, western corn ro otworms have developed resistance to cornsoybean rotation. In this biotype, named t he “eastern variant,” or “rotation resistant”,

adult females leave corn and fly to adjacent soybean fields to lay their eggs even

though soybean is not a host. When the field is rotated to corn the following year, the

eggs that were laid in the soil the previous summer hatch in the first-year corn and are

then able to cause economic damage.

c. In the Northern Region, northern corn rootworms have developed a biotype with

extended diapause. Intensive, annual rotation of corn with soybeans has resulted in

the selection of a biotype that has an ex tended egg diapause. Some eggs laid in corn

don't hatch the following growing season, but remain in diapause (a state of

dormancy) throughout another summer and winter to hatch when corn is planted in

the field nearly two or more years later. This region also represents a large area of

geographic overlap between western and northern corn rootworm.

# d. The Central Region represents intensiv e agriculture acreage and a variety of

rootworm pressure and species.

vi. Pioneer will be testing across a wide variety of corn rootworm pressure, from low to

moderate to severe. Rootworm pressure is not predictable from field to field and from

year to year. Therefore, many locations must be planted in order to ensure a wide variety

of rootworm pressure conditions will be evaluated. Enough locations must be tested to

adequately evaluate the impact of late-season lodging of refuge plants on grain quality.

vii. Pioneer will be testing across a wide vari ety of environments (11 states) since injury,

lodging and economic loss from corn rootworm is dependent on many environmental

factors. Other than soil type, environmental factors are unpredictable . Therefore, many

locations must be planted in order to ensure a wide variety of environmental conditions

will be sampled.

a. Soil types can influence the amount of corn rootworm infestation. More corn

rootworm eggs will be deposited in moist soil than dry soil, although severe oxygen

limitations caused by saturated soil or standing water can cause increased mortality of

corn rootworm larvae. The amount of sand, silt and clay in soil will affect the amount

of moisture the soil holds. The degree of soil compaction also influences rootworm

movement and survival.

b. Varying temperatures influence rootwo rm larvae movement and adult beetle flight

behavior, impacting the amount of larval root worm injury and silk clipping by adults.

The amount and timing of rainfall can also impa ct the extent of corn rootworm injury,

lodging, yield and harvestability. Under dry and heat-stressed conditions, plants

injured by corn rootworms suffer greater yield losses than when plants have adequate

moisture. When rainfall is adequate, the corn plant is able to get the moisture and soil

nutrients necessary to compensate for the root injury, and yield differences are less

pronounced.

c. Late-season thunderstorms are often acco mpanied by strong winds. This will cause

corn plants with rootworm injury to lodge. Concurrent diseases resulting in stalk rot

will also lead to lodging. The degree of this lodging depends on many factors,

including the degree of rootworm injury, soil mo isture, plant root architecture, velocity

and direction of wind, etc.

viii. Pioneer will be testing across a wide variety of farming practices and grower

demographics.

a. For the blended refuge system to be adopted by growers, Pioneer must confirm that

blended and refuge seed is plantable and manageable using a wide variety of

different equipment used by growers (for example, finger or air planter mechanism,

planters with or without insecticide boxes, combines with reels to pick up lodged corn,

etc.) and configurations (block or strip re fuge configurations). The performance of

59122 corn blended with non-Bt corn OAMRW as an in-field lepidopteran refuge for

1507x59122 corn blended with 1507 corn needs to be evaluated.

b. Depending on their farming operations, growers use different degrees of refuge

management and insecticides. Some growers treat their Bt and refuge corn

aggressively in order to obtain the highest pos sible yield. Other growers manage less

intensively. The amount, type and benefit of insecticide use vary greatly with different

farming practices. The blended refuge system needs to be tested across a wide

range of refuge management options.

c. Varying levels of technology accept ance among growers mean some growers will be

anxious to adopt the blended refuge system and others may be more reluctant. Large

acreage is needed to place blended refuge pr oducts with a variety of growers who

have different expectations of how a blended refuge product should perform and the

benefits it might bring them.

# G2. Participants in the Experimental Program

Please see CBI Cross Reference Number 2 in the Confidential Appendix.

G3. Acreage and Seed Quantities by State for the EUP Program

Pioneer has proposed it s acreage needs and field te sting program for 2010. As recommended in EPA’s

“Tips for Plant-Incorporated Protectant (PIP) Exper imental Use Permit (EUP) Program Submission”, this

acreage calculation includes the following categories:

i. PIP test material, including plants containing registered PIPs;

ii. non-PIP plants used for breeding purposes;

iii. non-PIP plants that are not intentional recipi ents of a PIP (e.g. isoline control plots); and

iv. associated border rows contained within t he test block(s), including border rows which

outline the perimeter of the test block(s).

Under this EUP, Pioneer will not be testing any Category ii or iv plants.

EUP test material consists of the one or more of the following:

• 1507x59122 corn blended with 1507 corn at a 5% blend rate

• 1507x59122 corn blended with 1507 corn at a 10% blend rate

• 59122 corn blended with non-Bt corn at a 5% blend rate

• 59122 corn blended with non-Bt corn at a 10% blend rate

Registered PIPs for comparison purposes:

• In some experiments, Herculex XTRA (HXX) and/or Herculex RW (HXRW) will be used for

comparison purposes.

Non-PIPs for comparison purposes:

• In some experiments, non-PIP plants will be included as a control to ascertain rootworm

pressure at a given location.

All of the individual events or br eeding stacks that comprise the bl ended refuge system are registered

by EPA.

• 1507: 29964-3

• 59122: 29964-4

• 1507x59122: 29964-5

All PIP proteins and inert ingredients have full tolerance exemptions.

• Cry1F: 40CFR §174.520

• Cry34/35: 40CFR §174.506

• PAT: 40CFR §174.522

# Table 1 provides the proposed acreage per state, and Table 2 provides the proposed acreage per

protocol. Tables 3 and 4 provide the proposed acreag e for each protocol, split out by state. Table 5

summarizes the amount of seed planted and the to tal estimated amount of active ingredient to be

used in the EUP Program.

Table 1. Proposed Acreage by State

EUP Test Material

(Acres) Registered PIP

Comparators

(Acres)

State A B C D HXX HXRW Non-PIP

Control

(Acres) Maximum

Total

Acres

IL 5160 40 10 5210

IN 5160 40 10 5210

IA 5160 40 10 5210

KS 1700 0 0 1700

MI 1700 0 0 1700

MN 5160 40 10 5210

MO 1700 0 0 1700

NE 5160 40 10 5210

OH 1700 0 0 1700

SD 1860 40 10 1910

WI 1860 40 10 1910

Total 36,320 280 70 36,670

A: 1507x59122 corn blended with 1507 corn (5% blend)

B: 1507x59122 corn blended with 1507 corn (10% blend)

C: 59122 corn blended with non-Bt corn (5% blend)

D: 59122 corn blended with non-Bt corn (10% blend)



# The exact breakout of EUP test material per state is not known at this time. The total EUP acres per

state are a maximum, but the allocation of EUP acre s between EUP test entries within a state is an

estimate.

# Table 2. Proposed Acreage by Protocol

EUP Test Material

(Acres) Registered PIP

Comparators

(Acres)

Protocol A B C D HXX HXRW Non-PIP

Control

(Acres) Maximum

Total

Acres

Grower

Evaluation

Protocol 35,200 0 0 35,200

Research

Trial

Protocol 1120 280 70 1,470

Total 36,320 280 70 36,670

A: 1507x59122 corn blended with 1507 corn (5% blend)

B: 1507x59122 corn blended with 1507 corn (10% blend)

C: 59122 corn blended with non-Bt corn (5% blend)

D: 59122 corn blended with non-Bt corn (10% blend)



# Notes:

i. The exact breakout of EUP test material, registered PIP comparators and non-PIP controls

per state is not known at this time. The alloca tion of EUP acres among EUP test entries within

a state is an estimate but the total EUP acres per state are a maximum.

# Table 3. Acreage by State for Grower Evaluation Protocol

EUP Test Material

(Acres)

State A B C D Maximum

Total Acres

IL 5000 5000

IN 5000 5000

IA 5000 5000

KS 1700 1700

MI 1700 1700

MN 5000 5000

MO 1700 1700

NE 5000 5000

OH 1700 1700

SD 1700 1700

WI 1700 1700

Total 35,200 35,200

A: 1507x59122 corn blended with 1507 corn (5% blend)

B: 1507x59122 corn blended with 1507 corn (10% blend)

C: 59122 corn blended with non-Bt corn (5% blend)

D: 59122 corn blended with non-Bt corn (10% blend)



# Notes:

i. There will be no registered PIP (HXX) or non-PI P material planted under this protocol.

ii. The exact breakout of EUP test material per state is not known at this time. The total EUP

acres per state are a maximum, but the alloca tion of EUP acres between EUP test entries

within a state is an estimate.

# Table 4. Acreage by State for Research Trial Protocol

EUP Test Material Registered PIP

Comparators

State A B C D HXX HXRW Non-PIP

Control Maximum

Total

Acres

IL 160 40 10 210

IN 160 40 10 210

IA 160 40 10 210

MN 160 40 10 210

NE 160 40 10 210

SD 160 40 10 210

WI 160 40 10 210

Total 1120 280 70 1470

A: 1507x59122 corn blended with 1507 corn (5% blend)

B: 1507x59122 corn blended with 1507 corn (10% blend)

C: 59122 corn blended with non-Bt corn (5% blend)

D: 59122 corn blended with non-Bt corn (10% blend)



# Notes:

ii. The exact breakout of EUP test material, registered PIP comparators and non-PIP controls

per state is not known at this time. The to tal EUP acres per state are a maximum, but the

allocation of EUP acres between EUP test entries within a state is an estimate.Table 5. Amount of Seed (g) and Active Ingredient (grams of Cry1F, Cry34Ab1 and Cry35Ab1) by StateKg of SeedAmount of Active Ingredient per State(grams)StateA1HXX2Cry1F Cry34Ab1 Cry35Ab1IL43,434 337 164.8 2633.2 141.4IN43,434 337 164.8 2633.2 141.4IA43,434 337 164.8 2633.2 141.4KS14,310 0 53.9 860.5 46.2MI14,310 0 53.9 860.5 46.2MN43,434 337 164.8 2633.2 141.4MO14,310 0 53.9 860.5 46.2NE43,434 337 164.8 2633.2 141.4OH14,310 0 53.9 860.5 46.2SD15,657 337 60.2 962.8 51.7WI15,657 337 60.2 962.8 51.7Total 305,724 2,357 1160.0 18533.9 995.5A: 1507x59122 corn blended with 1507 corn (5% blend)For calculation purposes: Assumed a worst case scenario of 100%of the EUP Test Plants were planted to 1507x59122 corn blended with 1507 corn (5% Blend)and 100% of the Registered PIP Comparators were planted to HXX.11507x59122: Cry1F = 0.0038 g/kg seed (dw); Cry34Ab1 = 0.0633 g/kg seed (dw) and Cry35Ab1 = 0.0034 g/kg seed (dw)1507: Cry1F = 0.0031 g/kg seed (dw) (TotalExtractable Protein values from label were converted to a dry weight basis)2HXX: Cry1F = 0.0038 g/kg seed (dw); Cry34Ab1 = 0.0633g/kg seed (dw) and Cry35Ab1 = 0.0034 g/kg seed (dw

# G4. Details of the Proposed EUP Program

Crop: Corn (Zea mays L.)

Target Pests:

Agrotis ipsilon (Black cutworm)

Helicoverpa zea (Corn earworm or Cotton bollworm)

Ostrinia nubilalis (European corn borer)

Spodoptera frugiperda (Fall armyworm)

Diatraea grandiosella (Southwestern corn borer)

Striacosta albicosta (Western bean cutworm)

Elasmopalpus lignosellus (Lesser corn stalk borer)

Diatrae crambidoides (Southern corn stalk borer)

Diatrea saccharalis (Sugarcane borer)

Diabrotica virgifera virgifera (Western corn rootworm)

Diabrotica barberi (Northern corn rootworm)

Diagrotica virgifera zeae (Mexican corn rootworm)

Major Geographic Areas: Eleven states, representing diverse geo graphic regions primarily in the

Corn Belt and other areas where there is potential crop injury from corn rootworm.

Sites: Pioneer research centers, pr ivate cooperators, and privately owned farms. EUP experiments

will not be located at EPA registered pesticide-produ cing establishments. All EUP experiments will be

planted on existing agricultural land.

Period of Use: March 1, 2010 through May 31, 2011.

G5. Universal Protocol Procedures

The following procedures will be used for all EUP Experiments.

Shipping and Labeling

• Each shipment containing EUP Test Material must contain a copy of the EUP Master Label.

• Seed will be shipped in seed bags or plastic bulk containers (“Proboxes”).

• Seed bags are typically destroyed by the grower after planting. Proboxes are returned to Pioneer,

cleaned, rinsed and re-used.

• Complete documentation will be kept of seed invoic ed (if applicable) and any returns. In addition, the

following documents will be retained: federal EUP approval letter, state EUP approval letter (if

applicable), EUP Master Label, Section G, and Grower Agreement (refuge compliance) if applicable.

Schedule

• Planting of experiments will generally take place from early March through the month of June.

Observations and harvesting generally occur after planting through August to November.

Submission of Cooperator Names

• As per 40 CFR 172.4(b)(v), the names and street addresses of all cooperators will be submitted

as soon as possible. Because of the large nu mber of expected cooperators for this EUP and the

possibility of cooperators dropping out or changing at the last minute, cooperator names may not

be available prior to planting. All cooperator names will be subm itted to EPA by August 1, 2010.

# • Prior to August 1, 2010, if federal, regional or state inspectors wish to conduct an EUP inspection,

they should request a current list of cooperators in their state or region from Tracy Rood, Sr.

Registration Manager for North America at Pioneer . A current and accurate list will be provided

within one working day.

o E-mail: tracy.rood@pioneer.com

o Office phone: 515-270-4036

o Mobile phone: 515-229-8923

(This information is also stated on the Master Label.)

Planting

• Seed will be planted by machine.

• No special cleaning of planter is required before or after planting OAM1 or OAMRW blends or any

other seed planted under this EUP.

Marking of EUP experiments

• Planting locations will be identified with stakes or, if equipment available, GPS coordinates.

Isolation

• No isolation is required because the events planted under this EUP are all registered by EPA

and deregulated by USDA. In addition, both t he Cry1F and Cry34/35 PIPs have full tolerance

exemptions.

Harvest Procedures

• Vegetative material or grain will be harvested by machine.

• Cleaning of harvest equipment is not required before or after harvesting OAM1 or OAMRW blends

or any other seed planted under this EUP.

Final disposition of harvested grain and vegetative material

• Grain and vegetative material harvested under this EUP may enter food/feed streams through

normal commercial channels.

Post-Trial Monitoring

• None required.

# 1. Grower Evaluation / Re fuge Deployment Protocol

A. General Objective

Validate agronomic performance and grower perc eption of blended refuge product concepts.

• Cooperators will receive 1-2 pallets containing ~12-30 bags of corn seed each. Each pallet

will include one or more of the following :

o 1507x59122 corn blended with 1507 corn (5% blend)

o 1507x59122 corn blended with 1507 corn (10% blend)

o 59122 corn blended with non-Bt corn (5% blend)

o 59122 corn blended with non-Bt corn (10% blend)

• ~400-800 total locations

• ~30-80 acres per location

• ~10-15 hybrid genetic backgrounds across the geographies

Note: A bag of seed plants approximately 2.7 acres.

Cooperators will be encouraged to plant some non- Bt acres of similar genetics (outside the scope

of the EUP) so they can evaluate the insect e fficacy of blended refuge products compared to nonBt products. The non-Bt acres may be managed fo r pest control however the cooperator wishes:

granular insecticide, liquid insectici de, and/or insecticide seed treatment.

B. General Description of Data Collected

• Some cooperators may compare the efficacy and agronomic performance of a 5% blend vs. 10%

blend. Alternatively, some cooperators may co mpare the efficacy of 1507x59122 corn blended

with 1507 corn as compared to 59122 corn blended with non-Bt corn against lepidopteran pests.

• Some cooperators may evaluate 59122 corn blended with non-Bt corn as a block or strip

lepidopteran refuge for 1507x59122 corn blended with 1507 corn.

Pioneer will use a third-party firm to collect data from a subset of the growers at one or more times

during the growing season. Because the surv ey has not been written yet, questions and data

being collected are not available at this time. However, in general, growers will be surveyed about

their expectations, perceptions , and experience with blended corn rootworm refuge product

concepts. Categorical, analyzable data will likely be collected on various endpoints including:

• Plantability

• Plant growth and development

• Lepidopteran insect damage in the 59122 blended with non-Bt corn acres

• Lodging

• Harvestability

• Yield

• Overall convenience relative to current refuge management options

• Grain characteristics (quality and consistenc y) across blends at physiological maturity

# 2 Research Trial Protocol

A. General Objective

Evaluate agronomic performance of 5% and 10% blended refuge products with various insecticide

seed treatments across a wide range of geographies (up to 7 states across the Corn Belt) and

diverse conditions.

• ~25-30 locations

• ~5-10 acres total per location

• Treatments

o 0% HXX

o 5% 1507x59122 corn blended with 1507 corn

o 10% 1507x59122 corn blended with 1507 corn

o 0% HXRW

o 5% 59122 corn blended with non-Bt corn

o 10% 59122 corn blended with non-Bt

o Untreated control to evaluate site pressure

• The 5% and 10% blended refuge products may be evaluated different with insecticide seed

treatments on refuge seeds (for example, Cruiser 250, Cruiser 500 or Poncho 1250)

Roots will be dug at each location to ascertain general site pressure as well as specific damage to

blended refuge plants with different insecticide seed treatments.

B General Description of Data Collected

• Early insect observations

• Early stand count

• Site pressure for corn rootworm (from untreated control)

• Cluster sampling of blended refuge plants to evaluate insecticide seed treatments from blend

treatments

• Mid- to late-season insect observations

• Lodging

• Harvestability

• Yield

# CONFIDENTIAL ATTACHMENT to

PHI-2009-118

{CBI Cross-Reference Number 1}