Title

Revised Application for an Experimental Use Permit for 1507x59122 Corn Blended with 1507 Corn 

and 59122 Corn Blended with Non-Bt Corn 

Data Requirement

40 CFR Part 172 

Authors

Jamie Staley and Tracy Rood 

Submission Date

December 10, 2009 

Submitter

Tracy A. Rood 

Pioneer Hi-Bred International, Inc. 

2450 SE Oak Tree Court 

Ankeny, IA 50021 

Submitter Reference Number

PHI-2009-118 

Volume 1 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 1 of 142

Volume 1

Table of Contents 

Page

Statement of Confidentiality Claim .......................................................................................................... 2 

EPA Form 8570-17 (Application for Experimental Use Permit)............................................................... 5 

EPA Form 8570-4 (Confidential Statement of Formula).......................................................................... 6 

EPA Form 8570-34 (Certification with Respect to Data Citation)............................................................ 7 

EPA Form 8570-35 (Data Matrix for 1507x59122 corn blended with 1507 corn) ................................... 8 

EPA Form 8570-35 (Data Matrix for 59122 corn blended with non-Bt corn)......................................... 61 

Section A. Product Chemistry............................................................................................................... 90 

Section B. Proposed Labels ................................................................................................................. 91 

Section C. Toxicity Data ....................................................................................................................... 99 

Section D. Residue Data .................................................................................................................... 100 

Section E. Effectiveness Data ............................................................................................................ 101 

Section F. Tolerance if Testing Involves Use in Food or Feed........................................................... 102 

Section G. Proposed Experimental Program...................................................................................... 103 

G.1. Acreage Justification ............................................................................................................ 103 

G.2. Participants in the Experimental Program............................................................................ 105 

G.3. Acreage and Seed Quantities by State for the EUP Program.............................................. 105 

G.4. Details of the Proposed EUP Program ................................................................................ 111 

G.5. Universal Protocol Procedures............................................................................................. 111 

Grower Evaluation Protocol .......................................................................................................... 113 

Research Trial Protocol................................................................................................................. 114 

Confidential Attachment....................................................................................................................... 115 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 3 of 142
List of Tables and Figures 

Tables Page

Table 1. Proposed Acreage by State.................................................................................................. 106 

Table 2. Proposed Acreage by Protocol............................................................................................. 107 

Table 3. Acreage by State for Grower Evaluation Protocol................................................................ 108 

Table 4. Acreage by State for Research Trial Protocol ...................................................................... 109

Table 5. Amount of Seed (kg) and Active Ingredient (grams of Cry1F, Cry34Ab1 and 

Cry35Ab1) by State ............................................................................................................................. 110 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 4 of 142

EPA Forms 8570-4

Confidential Statement of Formula 

{See CBI Cross Reference Number 1} 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 6 of 142

 

EPA Form 8570-35

Data Matrix for 1507x59122 corn blended with 1507 corn 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 8 of 142
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline Study Name MRID Number Submitter Sta

Acute oral toxicity in mice: Cry1F Bacillus thuringiensis subsp. aizawai delta endotoxin 44691101 68467 PEEffectiveness data for Bacillus thuringiensis var. aizawai Cry1F insect control protein as expressed in maize 44691102 68467 PEBackground document on resistance management 44691103 68467 PEProduct characterization data for Bacillus thuringiensis var. aizawai: Cry1F control protein as expressed in maize 44714801 68467 PE

Characterization of gene inserts – Bacillus thuringiensis aizawai: Cry1F control protein as expressed in maize 44714802 68467 PEEquivalency of microbial and maize expressed Cry1F protein; characterization of test substances for biochemical and toxicological studies. In vitro digestibility of microbial and maize expressed Cry1F protein under simulated gastric conditions 44714803 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Quantitative ELISA analysis of Cry1F expression levels in maize MPS inbred and hybrid lines 1360, 1365, 1366 and 1369 (interim report) 44714804 68467 PEComparison of the amino acid sequence similarity of Cry1F and PAT proteins to KnPER allergen protein 44971701 68467 PEMicrobial B.t. Cry1F (truncated) delta-endotoxin: Maize-insect-pest susceptibility study 45020101 68467 PECharacterization of inserted genes in Cry1F maize line 1507 45020102 68467 PECharacterization of expressed Cry1F protein in maize tissues (pollen, grain, grain-containing feed and purified maize-expressed Cry1F protein) and microbial expressed Cry1F delta-endotoxin by biological and biochemical procedures 45020103 68467 PE

Quantitative ELISA analysis of Cry1F and PAT expression levels in and compositional analysis of maize inbred and hybrid line 1362 and 1507 45020104 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Environmental fate of Cry1F protein incorporated into soil 45020105 68467 PECry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: an acute toxicity study with the earthworm in an artificial soil substrate 45020106 68467 PE

Chronic exposure of Folsomia candida to bacterially expressed Cry1F protein 45020107 68467 PE

OECD 207 

B.t. Cry1F delta endotoxin: A 48-hour static-renewal acute toxicity test with the Cladoceran (Daphnia magna) using bacterially expressed B.t. Cry1F delta endotoxin, and pollen from maize expressing B.t.

Cry1F delta endotoxin 45020108 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary study with green lacewing larvae 45020109 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary study with the ladybird beetle 45020110 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary toxicity study with parasitic hymenoptera 45020111 68467 PE

71-2 

Transgenic corn expressing Bacillus thuringiensis var. aizawai (B.t.) Cry1F delta endotoxin: A dietary toxicity study with Northern bobwhite 45020112 68467 PE

Field surveys of beneficial arthropods associated with Bacillus thuringiensis Cry1F maize 45020113 68467 PE

Efficacy of Cry1F events TC1360 and TC1507 45020114 68467 PECry1F binding studies 45020115 68467 PEResistance management plan for transgenic maize expressing the Cry1F insecticidal protein from Bacillus thuringiensis var. aizawai 45020116 68467 PE

Supplement to MRID 44714801: Supplemental data - Product characterization data for Bacillus thuringiensis var. aizawai: Cry1F control protein as expressed in maize 45020117 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Supplement to MRID 44691101: Supplemental data - Acute oral toxicity in mice: Bacillus thuringiensis var. aizawai: Cry1F delta endotoxin 45020118 68467 PEPhosphinothricin acetyltransferase (PAT) protein: In vitro digestibility study 45041501 68467 PE

Non-target exposure and risk assessment for environmental dispersal of Cry1F maize pollen 45041502 68467 PEEvaluation of the dietary effect(s) on honeybee development using bacterially expressed B.t. Cry1F 

delta endotoxin and pollen from maize expressing B.t. Cry1F delta endotoxin 45041503 68467 PE

Waiver request: Fish toxicity test with transgenic maize (corn) containing Bacillus thuringiensis var. aiaqoi (B.t.) Cry1F delta endotoxin 45044201 68467 PEHigh dose demonstration of Cry1F events TC1360 and TC1507: European corn borer 45131101 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Toxicity of the Cry1F protein to neonate larvae of the monarch buttefly (Danaus plexippus (Linneaus)) 45131102 68467 PEPublic interest document for Cry1F-protected corn 45131103 29964 OW

Thermolability of Cry1F (truncated) delta endotoxin 45274801 68467 PECompositional analysis of maize MPS hybrid line 1507 45274802 68467 PE

Cry1F lateral flow test kit procedure for analyzing Cry1F corn grain 45279301 68467 PEMethod validation report for the determination of Cry1F delta endotoxin protein in grain by EnzymeLinked Immunosorbent Assay 45279302 68467 PEPublic interest document for Cry1F protected corn 45298101 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Supplement to MRID 45131102: Supplemental data - High dose demonstration of Cry1F events TC1360 and TC1507: European corn borer 45307701 68467 PEWaiver request: Fish toxicity test to assess the potential effects of maize containing Bacillus thuringiensis var. aizawai (Bt) Cry1F insecticidal protein (ICP) in native fish 45307702 68467 PE

Supplement to MRID 45020109: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with green lacewing larvae 45307801 68467 PE

Supplement to MRID 45020110: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with green ladybird beetle 45307802 68467 PE

Supplement to MRID 45020111: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with parasitic hymenoptera 45307803 68467 PE

Supplement to MRID 45020106: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: An acute toxicity study with the earthworm in an artificial soil substrate 45307804 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Supplement to MRID 45041503: Evaluation of the dietary effect(s) on honeybee development using bacterially expressed B.t. Cry1F delta endotoxin and pollen from maize expressing B.t. Cry1F delta endotoxin 45307805 68467 PESupplement to MRID 45298101: Supplemental data - Public interest document for Cry1F protected corn 45309401 29964 OW

Exposure and risk assessment of Herculex I Bt field corn pollen to Karner blue butterfly 45512901 68467 PENutritional equivalency of B.t. Cry1F maize - poultry 

feeding study 45622001 68467 PE

Field survey of beneficial arthropods associated with Bacillus thuringiensis Cry1F maize 45648001 68467 PEField surveys of non-target invertebrate populations in B.t. corn 45652001 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Development and characterization of EnzymeLinked Immunosorbent Assay (ELISA) for detection of Cry1F protein 45685601 68467 PEIndependent laboratory validation of method GRM 02.13, determination of Cry1F delta endotoxin protein in corn grain by an of Enzyme-Linked Immunosorbent Assay 45685602 68467 PESupplement to MRID 45131102: Toxicity of the Cry1F protein to neonate larvae of the monarch butterfly (Danaus plexippus (Linneaus)) 45759701 68467 PE

Stewardship of Herculex I Insect Protection with respect to the secondary lepidopteran pest, western bean cutworm (Richia albicosta Smith) 45896601 29964 OWLack of cross reactivity between Cry1F protein in Herculex I maize and the dust mite Der p7 protein with human sera positive for Der p7-IgE 46444001 68467 PE

Monitoring the susceptibility of European corn borer to Cry1Ab and Cry1F Bt proteins: Results from the 2004 collections and diapausing larvae collected in 2003 46583101 80778 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Stewardship of event TC1507 maize with respect to the secondary lepidopteran pests lesser corn stalk borer (Elasmopalpus lignosellus Zeller), southern corn stalk borer (Diatraea crambidiodes Grote), and sugarcane borer (Biatraea saccharalis Fabricius) 46600201 68467 PESlide presentation summarizing European corn borer and Cry1F resistance monitoring update 46695801 68467 PEInsect resistance management compliance assurance program report for corn borer-protected Bt corn 46747801 80778 PE

Field surveys of non-target invertebrate populations in Bt corn: Supplement to MRID No. 45652001 46784601 80778 PEMonitoring the susceptibility of corn lepidopteran pests to Cry1Ab and Cry1F proteins: 2005 monitoring results 46874901 80778 PE

Research results on 2004 European corn borer collections from Hamilton County, Iowa: Cry1F 47011201 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Insect resistance management compliance assurance program report for corn borer-protected Bt corn, corn rootworm-protected Bt corn, and corn borer/corn rootworm protected stacked Bt corn 47044401 80778 PEMonitoring the susceptibility of corn lepidopteran pests to Cry1Ab and Cry1F proteins: 2006 monitoring results 47118401 80778 PESoil accumulation of Cry1F protein after three years of cropping with Herculex I corn 47120701 68467 PETC1507 maize and fall armyworm in Puerto Rico 47176001 68467 PEProduct characterization data for Bacillus thuringiensis PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins expressed in transgenic maize plants 45242201 68467 PE

Equivalency of microbial and maize-expressed PS149B1 proteins 45242203 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Microbial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study 45242204 68467 PEComparison of the amino acid sequence of the Bacillus thuringiensis strain PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins to KnPER protein allergens 45242205 68467 PECharacterization of Pseudomonas produced and transgenic maize expressed phosphinothricin acetyltransferase (PAT) protein 45242206 68467 PE

PS149B1 14 kDa protein: Acute oral toxicity study in CD-1 mice 45242207 68467 PEPS149B1 44 kDa protein: Acute oral toxicity study in CD-1 mice 45242208 68467 PEPS149B1 14 kDa and 44 kDa proteins: Acute oral toxicity study in CD-1 mice 45242209 68467 PE

PS149B1 binary insecticidal crystal protein: A dietary toxicity study with ladybird beetle 45242210 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

The tri-trophic interaction between PS149B1 transformed maize, corn leaf aphid and ladybird beetle 45242211 68467 PEIn vitro digestibility of PS149B1 proteins 45242212 68467 PEQuantitative ELISA analysis of PS149B1 protein expression levels in hybrid and inbred lines of maize 

event TC5639 (interim report) 45242213 68467 PE

Degradation of microbial binary PS149B1 delta endotoxin in a representative soil from the midWestern USA maize growing region 45242214 68467 PEProduct durability plan for transgenic maize expressing insecticidal crystal protein from Bacillus 

thuringiensis strain PS149B1 during the experimental use period 45242215 68467 PE

Field efficacy of PS149B1 maize events against corn rootworms 45242216 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Microbial PS149B1 binary insecticidal crystal proteins, pollen expressing PS149B1 binary insecticidal crystal proteins, and individual PS149B1 14 kDa and 44 kDa insecticidal crystal proteins 45340701 68467 PEPS149B1binary insecticidal crystal protein: Acute toxicity study to the earthworm in an artificial substrate 45360201 68464 PELateral flow test kit method validation for the detection of the PS149B1 14 kDa and 44 kDa protein in maize grain 45383401 68467 PEHeat lability of individual proteins of the PS149B1 binary ICP 45584501 68467 PEIn vitro simulated gastric fluid digestibility study of microbially derived Cry34Ab1 protein 45584502 68467 PE

Characterization of Cry34Ab1 and Cry35Ab1 from recombinant Pseudomonas flourescens and 

transgenic maize 45790401 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Characterization of DNA inserted into transgenic corn events (Cry34Ab1 and Cry35Ab1) 45790402 68467 PEPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout, Oncorhynchus mykiss Wallbaum 45790403 68467 PE

PS149B1 binary insecticidal crystal protein: Acute toxicity study with Daphnid, Daphnia magna Straus 45790404 68467 PEPS149B1 binary insecticidal crystal protein: Dietary toxicity to parasitic hymenoptera (Nasonia vitripennis) 45790405 68467 PE

Assessment of the chronic toxicity of diet containing Bacillus thuringiensis PS149B1 insecticidal crystal protein to collembolan (Folsomia candida) 45790406 68467 PEPS149B1 binary insecticidal crystal protein: Dietary toxicity to green lacewing (Chrysoperla carnea) 45790407 68467 PESDS-PAGE sensitivity analysis for Cry35Ab1 in support of the simulated gastric fluid digestibility study MRID 45242212 45790408 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Trait durability and experimental use of transgenic maize expressing the insecticidal crystalline proteins 

Cry34Ab1and Cry35Ab1 45790509 68467 PE

Field efficacy of Cry34Ab1/Cry35Ab1 maize events against corn rootworms 45790410 68467 PEProduct characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17658) 45790501 68467 PE

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 45790601 68467 PESummary heat lability studies with Cry34Ab1/Cry35Ab1 45808601 68467 PE

Quantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17658 45833101 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Quantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17662 45833102 68467 PESlide presentation summarizing Cry34Ab1/Cry35Ab1 heat inactivation studies 45860201 68467 PEProbe MOA studies to assess potential for protein synthesis inhibition by Bacillus thuringiensis

PS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbit reticulocyte assay: Re-examination of lab notebook data 45942801 68467 PE

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 46030001 68467 PE

Public interest document for Cry34/35Ab1 corn rootworm-protected corn 46122701 29964 OWIndependent laboratory validation Pioneer Hi-Bred International, Inc. ELISA method for the quantification of Cry34Ab1 protein from transgenic plants 46123901 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Independent laboratory validation of Dow AgroSciences method GRM 03.13, “ Determination of Cry35Ab1 insecticidal crystal protein in maize tissue by Enzyme-Linked Immunosorbent Assay” 46123902 68467 PECry34/35 protein distribution and familiarity 46123903 68467 PEAgronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry34Ab1, cry35Ab1 and pat genes: Chile locations 46123904 68467 PEBiological equivalency of Cry34/35Ab1 insecticidal crystal protein in transgenic plants derived from transgenic Pseudomonas flourscens 46123905 68467 PECharacterization of Cry34Ab and Cry35Ab1 proteins derived from transgenic maize event E4497.59.1.22 (DAS-59122-7) 46123906 68467 PE

Characterization of phosphinothricin acetyltransferase (PAT) derived from transgenic maize event E4497.59.1.22 46123907 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Characterization of DNA inserted into transgenic corn events DAS-45216-6 and DAS-59122-7 46123908 68467 PEDetailed characterization of DNA into transgenic corn events DAS-45216-6 and DAS-59122-7 46123909 68467 PEEvaluation of microbe derived Cry34Ab1 and Cry35Ab1 proteins for protein synthesis inhibition activity 46123910 68467 PENutritional equivalency study of maize containing Cry34Ab1 and Cry35Ab1: Poultry feeding study 46123911 68467 PEThe effect of Cry34Ab1/Cry35Ab1 proteins on the development and mortality of the ladybird beetle Coleomegilla maculata DeGeer 46123912 68467 PENon-target invertebrate ecological risk assessment for field corn expressing Cry34Ab1 and Cry35Ab1 insecticidal crystal proteins in event DAS-59122-7 46123913 68467 PEEvaluation of the impact corn rootworm control strategies on non-target arthropods 46123914 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Investigations into dose of Cry34Ab1/Cry35Ab1 rootworm-resistant maize event DAS-59122-7 against western and northern corn rootworms in support of trait durability plans 46123915 68467 PEEffect on western corn rootworm adults feeding on Cry34/35Ab1 corn rootworm-protected corn tissue and implication for product durability 46123916 68467 PEEvaluation of endangered/threatened insect species relative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids 46123917 68467 PETrait durability plan for Cry34/35 corn rootworm protected corn event DAS-59122-7 following commercialization 46123918 68467 PESimulations of corn rootworm adaptation to Cry34/35 corn rootworm protected corn in support of trait durability plans for event DAS-59122-7 46123919 68467 PEDigestion of allergenic and non-allergenic proteins in simulated gastric fluid 46123920 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Digestion efficiency of allergens and non-allergens in simulated gastric fluid: Bacillus thuringiensis

Cry34/35Ab1 construct PHP17662 46388601 68467 PE

Lack of Cry34Ab1/Cry35Ab1 co-association in solution 46556801 68467 PEEvaluation of the sequence similarities of the 

Cry34Ab1, Cry35Ab1 and PAT proteins to the public protein sequence datasets 46584701 68467 PE

Thermolability of PS149B1 binary delta endotoxin 45358401 68467 PESummary information in support of the Section 3 registration of Herculex XTRA Insect Protection corn 46297101 68467 PECharacterization of a stacked hybrid of transgenic corn lines DAS-01507-1 and DAS-59122-7 by comparison to the individual lines 46297102 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Agronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry1F, cry34Ab1, cry35Ab1 and pat genes: US and Canada locations 46297103 68467 PEAmended report:: Agronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry1F, cry34Ab1, cry35Ab1 and pat genes: US and Canada locations 46343801 68467 PENutritional equivalency study of stacked hybrid of transgenic maize line 1507 (event DAS-01507-1) and 59122 (event DAS-59122-7): Poultry feeding study 46343803 68467 PEComparison of insect efficacy and expression in Cry34/35 corn events DAS-59122-7 and DAS59132-8 expressed as a single and a stack with DAS-01507-1 46343804 68467 PEField efficacy of Herculex XTRA (DAS-01507-1xDAS-59122-7) insect resistant corn 46343805 68467 PEInvestigation of potential interaction between Cry1F and the binary Cry34Ab1/Cry35Ab1 proteins 46343806 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 1507x59122 corn ble

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

The effect of maize pollen expressing the Cry1F and Cry34Ab1/Cry35Ab1 proteins on the development and mortality of the ladybird beetle Coleomegilla maculata DeGeer 46343807 68467 PELaboratory bioassay assessment of corn pollen protein (Cry34/35 and Cry1F, stacked transgenes), and their effect on the larvae of monarch butterfly 46343808 68467 PEEquimolar ratio of Cry1F (tr) + Cry35Ab1 +Cry34Ab1: Acute oral toxicity study in CD-1 mice 46384301 68467 PEProposed revisions to IRM-related registration requirements for Cry1 Plant Incorporated Protectants in field corn 47407001 80778 PECharacterization of instar-specific response and larval recovery of western corn rootworm exposed to DAS-59122-7 maize 47356701 29964 OWEffect of off-type seeds on the precision of sublethal corn rootworm seedling assay results 47356702 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

An observational study characterizing the relative quantity and distribution of Cry34Ab1 and Cry35Ab1 insecticidal proteins in roots of maize containing event DAS-59122-7 47356703 29964 OWQuantifying the concentrations of Cry34Ab1 and Cry35Ab1 in 59122 maize grown in the sublethal corn rootworm seedling assay system 47356704 29964 OWField measures of adult corn rootworm (Coleoptera: 

Chrysomelidae) production when refuge is deployed as a seed blend with DAS-59122-7 maize 47356705 29964 OW

Impact of western and northern corn rootworm exposure to DAS-59122-7 in a block planting and a seed blend on life history traits of adults 47356706 29964 OWInvestigating the effect of insecticidal proteins Cry34/35Ab1 on western corn rootworm host searching behavior 47356707 29964 OWModeling durability of DAS-59122-7 maize efficacy against corn rootworm (Coleoptera: Chrysomelidae) when refuge is deployed as a seed blend 47356708 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Trait durability plan for Optimum® AcreMaxTM1 Insect Protection following commercialization 47356709 29964 OWBenefits document for Optimum® AcreMaxTM1 Insect Protection 47356710 29964 OWLiterature references for Optimum® AcreMaxTM1 Insect Protection 47356711 29964 OWAdditional responses for “Pioneer’s benefits 

document in support of a Sec(3) product registration for Optimum® AcreMaxTM1 Insect Protection, also known as stack Herculex XTRA 9Cry34/35Ab1 + Cry1F) with Herculex I (Cry1F refuge seed for CRW) [MRID 473567-10 and supplemental data]” 47683801 29964 OW

Insecticidal seed treatments for corn rootworm: Inconsistent protection for refuge plants 47789402 29964 OWAddendum to the trait durability plan for Optimum® AcreMaxTM1 Insect Protection following commercialization 47789403 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Literature references supporting the additional studies for Optimum® AcreMaxTM1 Insect Protection 47789404 29964 OWAddendum to the Benefits of Optimum® AcreMaxTM1 47795101 29964 OWAddendum to: A spatially explicit computer simulation study of corn rootworm, Diabroticaspp.[Coleoptera: Chrysomelidae] 47871001 29964 OWLiterature references cited in the modeling report PHI-2009-125 47871002 29964 OWAdditional addendum to MRID 473567-08: A spatially explicit computer simulation study of corn rootworm, Diabrotica spp.[Coleoptera: Chrysomelidae] 47887901 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE29964 OW68467 PE68467 PE68467 PE68467 PE29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE29964 OW68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE29964 OW68467 PE80778 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE80778 PE80778 PE80778 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

80778 PE80778 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

2996-EUP- 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122 corn ble

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE29964 OW68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE80778 PE29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:1507x59122 corn blen

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
 

EPA Form 8570-35

Data Matrix for 59122 corn blended with non-Bt corn 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 61 of 142
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins expressed in transgenic maize plants 45242201 68467 PEEquivalency of microbial and maize-expressed PS149B1 proteins 45242203 68467 PEMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study 45242204 68467 PEComparison of the amino acid sequence of the Bacillus thuringiensis strain PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins to KnPER protein allergens 45242205 68467 PECharacterization of Pseudomonas produced and transgenic maize expressed phosphinothricin acetyltransferase (PAT) protein 45242206 68467 PE

PS149B1 14 kDa protein: Acute oral toxicity study in CD-1 mice 45242207 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

PS149B1 44 kDa protein: Acute oral toxicity study in CD-1 mice 45242208 68467 PEPS149B1 14 kDa and 44 kDa proteins: Acute oral toxicity study in CD-1 mice 45242209 68467 PEPS149B1 binary insecticidal crystal protein: A dietary toxicity study with ladybird beetle 45242210 68467 PEThe tri-trophic interaction between PS149B1 transformed maize, corn leaf aphid and ladybird beetle 45242211 68467 PEIn vitro digestibility of PS149B1 proteins 45242212 68467 PEQuantitative ELISA analysis of PS149B1 protein expression levels in hybrid and inbred lines of maize 

event TC5639 (interim report) 45242213 68467 PE

Degradation of microbial binary PS149B1 delta endotoxin in a representative soil from the midWestern USA maize growing region 45242214 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product durability plan for transgenic maize expressing insecticidal crystal protein from Bacillus 

thuringiensis strain PS149B1 during the experimental use period 45242215 68467 PE

Field efficacy of PS149B1 maize events against corn rootworms 45242216 68467 PEMicrobial PS149B1 binary insecticidal crystal proteins, pollen expressing PS149B1 binary insecticidal crystal proteins, and individual PS149B1 14 kDa and 44 kDa insecticidal crystal proteins 45340701 68467 PEPS149B1binary insecticidal crystal protein: Acute toxicity study to the earthworm in an artificial substrate 45360201 68464 PELateral flow test kit method validation for the detection of the PS149B1 14 kDa and 44 kDa protein in maize grain 45383401 68467 PEHeat lability of individual proteins of the PS149B1 binary ICP 45584501 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

In vitro simulated gastric fluid digestibility study of microbially derived Cry34Ab1 protein 45584502 68467 PE

Characterization of Cry34Ab1 and Cry35Ab1 from recombinant Pseudomonas flourescens and 

transgenic maize 45790401 68467 PE

Characterization of DNA inserted into transgenic corn events (Cry34Ab1 and Cry35Ab1) 45790402 68467 PEPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout, Oncorhynchus mykiss Wallbaum 45790403 68467 PEPS149B1 binary insecticidal crystal protein: Acute toxicity study with Daphnid, Daphnia magna Straus 45790404 68467 PEPS149B1 binary insecticidal crystal protein: Dietary toxicity to parasitic hymenoptera (Nasonia vitripennis) 45790405 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Assessment of the chronic toxicity of diet containing Bacillus thuringiensis PS149B1 insecticidal crystal protein to collembolan (Folsomia candida) 45790406 68467 PEPS149B1 binary insecticidal crystal protein: Dietary toxicity to green lacewing (Chrysoperla carnea) 45790407 68467 PE

SDS-PAGE sensitivity analysis for Cry35Ab1 in support of the simulated gastric fluid digestibility study MRID 45242212 45790408 68467 PETrait durability and experimental use of transgenic maize expressing the insecticidal crystalline proteins Cry34Ab1and Cry35Ab1 45790509 68467 PE

Field efficacy of Cry34Ab1/Cry35Ab1 maize events against corn rootworms 45790410 68467 PEProduct characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17658) 45790501 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry1F and Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 45790601 68467 PESummary heat lability studies with Cry34Ab1/Cry35Ab1 45808601 68467 PEQuantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17658 45833101 68467 PE

Quantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17662 45833102 68467 PESlide presentation summarizing Cry34Ab1/Cry35Ab1 heat inactivation studies 45860201 68467 PE

Probe MOA studies to assess potential for protein synthesis inhibition by Bacillus thuringiensis

PS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbit reticulocyte assay: Re-examination of lab notebook data 45942801 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 46030001 68467 PEPublic interest document for Cry34/35Ab1 corn rootworm-protected corn 46122701 29964 OWIndependent laboratory validation Pioneer Hi-Bred International, Inc. ELISA method for the quantification of Cry34Ab1 protein from transgenic plants 46123901 68467 PEIndependent laboratory validation of Dow AgroSciences method GRM 03.13, “ Determination of Cry35Ab1 insecticidal crystal protein in maize tissue by Enzyme-Linked Immunosorbent Assay” 46123902 68467 PE

Cry34/35 protein distribution and familiarity 46123903 68467 PEAgronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry34Ab1, cry35Ab1 and pat genes: Chile locations 46123904 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Biological equivalency of Cry34/35Ab1 insecticidal crystal protein in transgenic plants derived from transgenic Pseudomonas flourscens 46123905 68467 PECharacterization of Cry34Ab and Cry35Ab1 proteins derived from transgenic maize event E4497.59.1.22 (DAS-59122-7) 46123906 68467 PECharacterization of phosphinothricin acetyltransferase (PAT) derived from transgenic maize event E4497.59.1.22 46123907 68467 PECharacterization of DNA inserted into transgenic corn events DAS-45216-6 and DAS-59122-7 46123908 68467 PEDetailed characterization of DNA into transgenic corn events DAS-45216-6 and DAS-59122-7 46123909 68467 PE

Evaluation of microbe derived Cry34Ab1 and Cry35Ab1 proteins for protein synthesis inhibition activity 46123910 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Nutritional equivalency study of maize containing Cry34Ab1 and Cry35Ab1: Poultry feeding study 46123911 68467 PEThe effect of Cry34Ab1/Cry35Ab1 proteins on the development and mortality of the ladybird beetle Coleomegilla maculata DeGeer 46123912 68467 PENon-target invertebrate ecological risk assessment for field corn expressing Cry34Ab1 and Cry35Ab1 insecticidal crystal proteins in event DAS-59122-7 46123913 68467 PEEvaluation of the impact corn rootworm control strategies on non-target arthropods 46123914 68467 PEInvestigations into dose of Cry34Ab1/Cry35Ab1 rootworm-resistant maize event DAS-59122-7 against western and northern corn rootworms in support of trait durability plans 46123915 68467 PEEffect on western corn rootworm adults feeding on Cry34/35Ab1 corn rootworm-protected corn tissue and implication for product durability 46123916 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of endangered/threatened insect species relative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids 46123917 68467 PETrait durability plan for Cry34/35 corn rootworm protected corn event DAS-59122-7 following commercialization 46123918 68467 PESimulations of corn rootworm adaptation to Cry34/35 corn rootworm protected corn in support of trait durability plans for event DAS-59122-7 46123919 68467 PEDigestion of allergenic and non-allergenic proteins in simulated gastric fluid 46123920 68467 PEDigestion efficiency of allergens and non-allergens in simulated gastric fluid: Bacillus thuringiensis

Cry34/35Ab1 construct PHP17662 46388601 68467 PE

Lack of Cry34Ab1/Cry35Ab1 co-association in solution 46556801 68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the sequence similarities of the 

Cry34Ab1, Cry35Ab1 and PAT proteins to the public protein sequence datasets 46584701 68467 PE

Thermolability of PS149B1 binary delta endotoxin 45358401 68467 PEProposed revisions to IRM-related registration requirements for Cry1 Plant Incorporated Protectants in field corn 47407001 80778 PECharacterization of instar-specific response and larval recovery of western corn rootworm exposed to DAS-59122-7 maize 47356701 29964 OWEffect of off-type seeds on the precision of sublethal corn rootworm seedling assay results 47356702 29964 OWAn observational study characterizing the relative quantity and distribution of Cry34Ab1 and Cry35Ab1 insecticidal proteins in roots of maize containing event DAS-59122-7 47356703 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Quantifying the concentrations of Cry34Ab1 and Cry35Ab1 in 59122 maize grown in the sublethal corn rootworm seedling assay system 47356704 29964 OWField measures of adult corn rootworm (Coleoptera: 

Chrysomelidae) production when refuge is deployed as a seed blend with DAS-59122-7 maize 47356705 29964 OW

Impact of western and northern corn rootworm exposure to DAS-59122-7 in a block planting and a seed blend on life history traits of adults 47356706 29964 OWInvestigating the effect of insecticidal proteins Cry34/35Ab1 on western corn rootworm host searching behavior 47356707 29964 OWModeling durability of DAS-59122-7 maize efficacy against corn rootworm (Coleoptera: Chrysomelidae) when refuge is deployed as a seed blend 47356708 29964 OWTrait durability plan for Optimum® AcreMaxTM1 Insect Protection following commercialization 47356709 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Benefits document for Optimum® AcreMaxTM1 Insect Protection 47356710 29964 OWLiterature references for Optimum® AcreMaxTM1 Insect Protection 47356711 29964 OWAdditional responses for “Pioneer’s benefits 

document in support of a Sec(3) product registration for Optimum® AcreMaxTM1 Insect Protection, also known as stack Herculex XTRA 9Cry34/35Ab1 + Cry1F) with Herculex I (Cry1F refuge seed for CRW) [MRID 473567-10 and supplemental data]” 47683801 29964 OW

Insecticidal seed treatments for corn rootworm: Inconsistent protection for refuge plants 47789402 29964 OWAddendum to the trait durability plan for Optimum® AcreMaxTM1 Insect Protection following commercialization 47789403 29964 OWLiterature references supporting the additional studies for Optimum® AcreMaxTM1 Insect Protection 47789404 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Addendum to the Benefits of Optimum® AcreMaxTM1 47795101 29964 OWAddendum to: A spatially explicit computer simulation study of corn rootworm, Diabroticaspp.[Coleoptera: Chrysomelidae] 47871001 29964 OWLiterature references cited in the modeling report PHI-2009-125 47871002 29964 OWAdditional addendum to MRID 473567-08: A spatially explicit computer simulation study of corn rootworm, Diabrotica spp.[Coleoptera: Chrysomelidae] 47887901 29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE29964 OW68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE80778 PE29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: December 10, 2009 EPA Reg. No. / File Symb

29964-EUP-I 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:59122 corn blended w

Ingredient Bacillus thuringiensis Cry34/35Ab1 insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Tracy A. Rood  Sr. Registration Manager 

Date 

Decembe

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118
Section A. Product Chemistry 

Pioneer is developing a new blended seed product that controls Lepidopteran and corn rootworm 

pests and contains a built-in (i.e. “in the bag”) corn rootworm refuge. The product consists of a high 

percentage of 1507x59122 combined trait seed and a low percentage of refuge seed (1507 corn, 

which does not contain a Bt trait that controls corn rootworm) blended together in a package of seed. 

An additional product, 59122 corn blended with non-Bt corn is also being evaluated as a blended seed 

product that controls corn rootworm pests and contains an “in the bag” corn rootworm refuge. The 

blended 59122 product consists of a high percentage of 59122 corn blended with a low percentage of 

non-Bt refuge corn in a package of seed. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 90 of 142
Section B: Proposed Experimental Use Permit Labels 

The following pages contain the proposed Master Label for the EUP, including labels for the following 

EUP Test Plants: 

• 1507x59122 corn blended with 1507 corn (5% Blend) 

• 1507x59122 corn blended with 1507 corn (10% Blend) 

• 59122 corn blended with non-Bt corn (5% Blend) 

• 59122 corn blended with non-Bt corn (10% Blend) 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 91 of 142
Master Label 

29964-EUP-I 

Subparts: 

1) 1507x59122 Corn Blended with 1507 Corn (5% Blend) 

2) 1507x59122 Corn Blended with 1507 Corn (10% Blend) 

3) 59122 Corn Blended with Non-Bt Corn (5% Blend) 

4) 59122 Corn Blended with Non-Bt Corn (10% Blend) 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 92 of 142
1) 1507x59122 Corn Blended with 1507 Corn 

(5% Blend) 

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients of Component 1 (1507x59122 corn) : 95% of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1................................................................................................ <0.00038%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ................................................................................................. <0.00633%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ................................................................................................. <0.00034%* 

Inert Ingredient:

Phosphinothricin acetyltransferase produced by the pat gene and the genetic material 

necessary for its production in corn.................................................................................................................................. <0.00004%* 

Active Ingredients of Component 2 (1507 corn) : 5 % of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1...............................................................................................<0.01415%** 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn...................................................................................................................................<0.0020%** 

* % total protein on a dry wt. basis as expressed in grain 

** % total extractable protein as expressed in grain

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: 515-270-4036 

7300 NW 62nd Ave Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 93 of 142
DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 94 of 142
2) 1507x59122 Corn Blended with 1507 Corn 

(10% Blend) 

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients of Component 1 (1507x59122 corn) : 95% of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1................................................................................................ <0.00038%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ................................................................................................. <0.00633%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ................................................................................................. <0.00034%* 

Inert Ingredient:

Phosphinothricin acetyltransferase produced by the pat gene and the genetic material 

necessary for its production in corn.................................................................................................................................. <0.00004%* 

Active Ingredients of Component 2 (1507 corn) : 5 % of maize kernels

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1...............................................................................................<0.01415%** 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn...................................................................................................................................<0.0020%** 

* % total protein on a dry wt. basis as expressed in grain 

** % total extractable protein as expressed in grain

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc Phone: 515-270-4036 

7300 NW 62nd Ave. Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

Page 95 of 142
DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

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PHI-2009-118

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3) 59122 Corn Blended with Non-Bt Corn 

(5% Blend) 

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain corn rootworm pests: Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ................................................................................................... <0.0117%* 

Bacillus thuringiensis Cry35Ab1protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ............................................................................................... <0.000161%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn.............................................................................................................................. <0.0000069%* 

* % total protein on a dry wt. basis as expressed in grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc Phone: 515-270-4036 

7300 NW 62nd Ave. Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

PHI-2009-118

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4) 59122 Corn Blended with Non-Bt Corn 

(10% Blend) 

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-I 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain corn rootworm pests: Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ................................................................................................... <0.0117%* 

Bacillus thuringiensis Cry35Ab1protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7 ............................................................................................... <0.000161%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn.............................................................................................................................. <0.0000069%* 

* % total protein on a dry wt. basis as expressed in grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc Phone: 515-270-4036 

7300 NW 62nd Ave. Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

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Section C. Toxicity Data 

The environmental, human and animal safety of Cry1F and Cry34/35Ab1 insecticidal proteins has 

been previously reviewed by EPA for events 1507 and 59122 and concluded to have no adverse 

effects (U.S. EPA, 2001 & 2005). These proteins were granted an exemption from the requirement 

of a tolerance in corn by EPA on June 6, 2001 (40CFR §174.520) and September 21, 2005 (40CFR 

§174.506), respectively. 

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Section D. Residue Data 

Residue data are not applicable as permanent tolerance exemptions exist for Cry1F (40CFR 

§174.520) and Cry34/35Ab1 proteins (40CFR §174.506). 

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Section E. Effectiveness Data 

Not required under 40CFR §158.202(i). 

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Section F. Tolerance if Testing Involves Use in Food or Feed 

Cry1F protein is exempt from the requirement of a tolerance in corn: 40CFR §174.520. 

Cry34Ab1 and Cry35Ab1 proteins are exempt from the requirement of a tolerance in corn: 40CFR 

§174.506. 

Phosphinothricin acetyltransferase (PAT is exempt from the requirement of a tolerance in all plants: 

40CFR §174.522. 

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Section G. Proposed Experimental Program 

G1. Acreage Justification 

Pioneer is requesting a total of 36,670 acres in 11 states for 2010 in order to continue research, 

testing and evaluation of blended refuge concepts. 

This acreage is justified because of the wide diversity of environments and conditions where 

1507x59122 corn blended with 1507 corn and 59122 corn blended with non-Bt corn will be deployed. 

Experiments will be grown across diverse growing environments with a cross-section of cooperators 

and defined research protocols. The result will be data and observations necessary for product 

characterization, evaluation and advancement and product stewardship. Specific justification includes 

the following: 

i. Placement of experiments with cooperators will not be finalized until spring, since weather 

conditions can significantly affect planting locations. Cool and wet conditions may force 

cooperators to alter their planting intentions. Experiments may need to be moved within a 

state or across state lines. Therefore, the proposed EUP acreage needs to have the 

flexibility to allow for the possibility of experiments being shifted among locations and 

states. 

ii. Pioneer may be testing various insecticide seed treatments (ISTs), including a low/base 

rate (i.e., non-rootworm rate) of Cruiser IST and high rates of Cruiser and/or Poncho ISTs. 

iii. Pioneer will be testing approximately 10-15 different hybrid platforms in order to 

understand how blends perform in different genetic backgrounds in varying field and 

agronomic conditions. 

iv. Pioneer will be testing efficacy of blended refuge concepts against different rootworm 

species and unique rootworm biotypes: 

• Diabrotica virgifera virgifera (western corn rootworm), including the organophosphateresistant biotypes in the western Corn Belt and the eastern variant in the eastern Corn 

Belt, 

• Diabrotica barberi (northern corn rootworm), including areas of extended egg 

diapause; and 

• Geographies with mixed northern and western corn rootworm populations. 

v. Pioneer will be testing in up to 11 states. There are four major populations/biotypes of 

corn rootworm that encompass overlapping geographic regions. 

a. In the Western Region, western corn rootworms have developed a biotype that is 

resistant to organophosphate insecticides. 

b. In the Eastern Region, western corn rootworms have developed resistance to cornsoybean rotation. In this biotype, named the “eastern variant,” or “rotation resistant”, 

adult females leave corn and fly to adjacent soybean fields to lay their eggs even 

though soybean is not a host. When the field is rotated to corn the following year, the 

eggs that were laid in the soil the previous summer hatch in the first-year corn and are 

then able to cause economic damage. 

c. In the Northern Region, northern corn rootworms have developed a biotype with 

extended diapause. Intensive, annual rotation of corn with soybeans has resulted in 

the selection of a biotype that has an extended egg diapause. Some eggs laid in corn 

don't hatch the following growing season, but remain in diapause (a state of 

dormancy) throughout another summer and winter to hatch when corn is planted in 

the field nearly two or more years later. This region also represents a large area of 

geographic overlap between western and northern corn rootworm. 

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d. The Central Region represents intensive agriculture acreage and a variety of 

rootworm pressure and species. 

vi. Pioneer will be testing across a wide variety of corn rootworm pressure, from low to 

moderate to severe. Rootworm pressure is not predictable from field to field and from 

year to year. Therefore, many locations must be planted in order to ensure a wide variety 

of rootworm pressure conditions will be evaluated. Enough locations must be tested to 

adequately evaluate the impact of late-season lodging of refuge plants on grain quality. 

vii. Pioneer will be testing across a wide variety of environments (11 states) since injury, 

lodging and economic loss from corn rootworm is dependent on many environmental 

factors. Other than soil type, environmental factors are unpredictable. Therefore, many 

locations must be planted in order to ensure a wide variety of environmental conditions 

will be sampled. 

a. Soil types can influence the amount of corn rootworm infestation. More corn 

rootworm eggs will be deposited in moist soil than dry soil, although severe oxygen 

limitations caused by saturated soil or standing water can cause increased mortality of 

corn rootworm larvae. The amount of sand, silt and clay in soil will affect the amount 

of moisture the soil holds. The degree of soil compaction also influences rootworm 

movement and survival. 

b. Varying temperatures influence rootworm larvae movement and adult beetle flight 

behavior, impacting the amount of larval rootworm injury and silk clipping by adults. 

The amount and timing of rainfall can also impact the extent of corn rootworm injury, 

lodging, yield and harvestability. Under dry and heat-stressed conditions, plants 

injured by corn rootworms suffer greater yield losses than when plants have adequate 

moisture. When rainfall is adequate, the corn plant is able to get the moisture and soil 

nutrients necessary to compensate for the root injury, and yield differences are less 

pronounced. 

c. Late-season thunderstorms are often accompanied by strong winds. This will cause 

corn plants with rootworm injury to lodge. Concurrent diseases resulting in stalk rot 

will also lead to lodging. The degree of this lodging depends on many factors, 

including the degree of rootworm injury, soil moisture, plant root architecture, velocity 

and direction of wind, etc. 

viii. Pioneer will be testing across a wide variety of farming practices and grower 

demographics. 

a. For the blended refuge system to be adopted by growers, Pioneer must confirm that 

blended and refuge seed is plantable and manageable using a wide variety of 

different equipment used by growers (for example, finger or air planter mechanism, 

planters with or without insecticide boxes, combines with reels to pick up lodged corn, 

etc.) and configurations (block or strip refuge configurations). The performance of 

59122 corn blended with non-Bt corn OAMRW as an in-field lepidopteran refuge for 

1507x59122 corn blended with 1507 corn needs to be evaluated. 

b. Depending on their farming operations, growers use different degrees of refuge 

management and insecticides. Some growers treat their Bt and refuge corn 

aggressively in order to obtain the highest possible yield. Other growers manage less 

intensively. The amount, type and benefit of insecticide use vary greatly with different 

farming practices. The blended refuge system needs to be tested across a wide 

range of refuge management options. 

c. Varying levels of technology acceptance among growers mean some growers will be 

anxious to adopt the blended refuge system and others may be more reluctant. Large 

acreage is needed to place blended refuge products with a variety of growers who 

have different expectations of how a blended refuge product should perform and the 

benefits it might bring them. 

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G2. Participants in the Experimental Program 

Please see CBI Cross Reference Number 2 in the Confidential Appendix. 

G3. Acreage and Seed Quantities by State for the EUP Program 

Pioneer has proposed its acreage needs and field testing program for 2010. As recommended in EPA’s 

“Tips for Plant-Incorporated Protectant (PIP) Experimental Use Permit (EUP) Program Submission”, this 

acreage calculation includes the following categories: 

i. PIP test material, including plants containing registered PIPs; 

ii. non-PIP plants used for breeding purposes; 

iii. non-PIP plants that are not intentional recipients of a PIP (e.g. isoline control plots); and 

iv. associated border rows contained within the test block(s), including border rows which 

outline the perimeter of the test block(s). 

Under this EUP, Pioneer will not be testing any Category ii or iv plants. 

EUP test material consists of the one or more of the following: 

• 1507x59122 corn blended with 1507 corn at a 5% blend rate 

• 1507x59122 corn blended with 1507 corn at a 10% blend rate 

• 59122 corn blended with non-Bt corn at a 5% blend rate 

• 59122 corn blended with non-Bt corn at a 10% blend rate 

Registered PIPs for comparison purposes: 

• In some experiments, Herculex XTRA (HXX) and/or Herculex RW (HXRW) will be used for 

comparison purposes. 

Non-PIPs for comparison purposes: 

• In some experiments, non-PIP plants will be included as a control to ascertain rootworm 

pressure at a given location. 

All of the individual events or breeding stacks that comprise the blended refuge system are registered 

by EPA. 

• 1507: 29964-3 

• 59122: 29964-4 

• 1507x59122: 29964-5 

All PIP proteins and inert ingredients have full tolerance exemptions. 

• Cry1F: 40CFR §174.520 

• Cry34/35: 40CFR §174.506 

• PAT: 40CFR §174.522 

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Table 1 provides the proposed acreage per state, and Table 2 provides the proposed acreage per 

protocol. Tables 3 and 4 provide the proposed acreage for each protocol, split out by state. Table 5 

summarizes the amount of seed planted and the total estimated amount of active ingredient to be 

used in the EUP Program. 

Table 1. Proposed Acreage by State 

EUP Test Material 

(Acres) 

Registered PIP 

Comparators 

(Acres) 

State A B C D HXX HXRW 

Non-PIP 

Control 

(Acres) 

Maximum 

Total 

Acres 

IL 5160 40 10 5210 

IN 5160 40 10 5210 

IA 5160 40 10 5210 

KS 1700 0 0 1700 

MI 1700 0 0 1700 

MN 5160 40 10 5210 

MO 1700 0 0 1700 

NE 5160 40 10 5210 

OH 1700 0 0 1700 

SD 1860 40 10 1910 

WI 1860 40 10 1910 

Total 36,320 280 70 36,670 

A: 1507x59122 corn blended with 1507 corn (5% blend) 

B: 1507x59122 corn blended with 1507 corn (10% blend) 

C: 59122 corn blended with non-Bt corn (5% blend) 

D: 59122 corn blended with non-Bt corn (10% blend)

The exact breakout of EUP test material per state is not known at this time. The total EUP acres per 

state are a maximum, but the allocation of EUP acres between EUP test entries within a state is an 

estimate. 

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Table 2. Proposed Acreage by Protocol 

EUP Test Material 

(Acres) 

Registered PIP 

Comparators 

(Acres) 

Protocol A B C D HXX HXRW 

Non-PIP 

Control 

(Acres) 

Maximum 

Total 

Acres 

Grower 

Evaluation 

Protocol 

35,200 0 0 35,200 

Research 

Trial 

Protocol 

1120 280 70 1,470 

Total 36,320 280 70 36,670 

A: 1507x59122 corn blended with 1507 corn (5% blend) 

B: 1507x59122 corn blended with 1507 corn (10% blend) 

C: 59122 corn blended with non-Bt corn (5% blend) 

D: 59122 corn blended with non-Bt corn (10% blend)

Notes: 

i. The exact breakout of EUP test material, registered PIP comparators and non-PIP controls 

per state is not known at this time. The allocation of EUP acres among EUP test entries within 

a state is an estimate but the total EUP acres per state are a maximum. 

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Table 3. Acreage by State for Grower Evaluation Protocol 

EUP Test Material 

(Acres) 

State A B C D

Maximum 

Total Acres 

IL 5000 5000 

IN 5000 5000 

IA 5000 5000 

KS 1700 1700 

MI 1700 1700 

MN 5000 5000 

MO 1700 1700 

NE 5000 5000 

OH 1700 1700 

SD 1700 1700 

WI 1700 1700 

Total 35,200 35,200 

A: 1507x59122 corn blended with 1507 corn (5% blend) 

B: 1507x59122 corn blended with 1507 corn (10% blend) 

C: 59122 corn blended with non-Bt corn (5% blend) 

D: 59122 corn blended with non-Bt corn (10% blend)

Notes: 

i. There will be no registered PIP (HXX) or non-PIP material planted under this protocol. 

ii. The exact breakout of EUP test material per state is not known at this time. The total EUP 

acres per state are a maximum, but the allocation of EUP acres between EUP test entries 

within a state is an estimate. 

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Table 4. Acreage by State for Research Trial Protocol 

EUP Test Material 

Registered PIP 

Comparators 

State A B C D HXX HXRW 

Non-PIP 

Control 

Maximum 

Total 

Acres 

IL 160 40 10 210 

IN 160 40 10 210 

IA 160 40 10 210 

MN 160 40 10 210 

NE 160 40 10 210 

SD 160 40 10 210 

WI 160 40 10 210 

Total 1120 280 70 1470 

A: 1507x59122 corn blended with 1507 corn (5% blend) 

B: 1507x59122 corn blended with 1507 corn (10% blend) 

C: 59122 corn blended with non-Bt corn (5% blend) 

D: 59122 corn blended with non-Bt corn (10% blend)

Notes: 

ii. The exact breakout of EUP test material, registered PIP comparators and non-PIP controls 

per state is not known at this time. The total EUP acres per state are a maximum, but the 

allocation of EUP acres between EUP test entries within a state is an estimate. 

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Table 5. Amount of Seed (g) and Active Ingredient (grams of Cry1F, Cry34Ab1 and Cry35Ab1) by State 

Kg of Seed Amount of Active Ingredient per State (grams) 

State 

A1 HXX2 Cry1F Cry34Ab1 Cry35Ab1 

IL 43,434 337 164.8 2633.2 141.4 

IN 43,434 337 164.8 2633.2 141.4 

IA 43,434 337 164.8 2633.2 141.4 

KS 14,310 0 53.9 860.5 46.2 

MI 14,310 0 53.9 860.5 46.2 

MN 43,434 337 164.8 2633.2 141.4 

MO 14,310 0 53.9 860.5 46.2 

NE 43,434 337 164.8 2633.2 141.4 

OH 14,310 0 53.9 860.5 46.2 

SD 15,657 337 60.2 962.8 51.7 

WI 15,657 337 60.2 962.8 51.7 

Total 305,724 2,357 1160.0 18533.9 995.5 

A: 1507x59122 corn blended with 1507 corn (5% blend) For calculation purposes: Assumed a worst case scenario of 100% of the EUP Test Plants were planted to 1507x59122 corn blended wiand 100% of the Registered PIP Comparators were planted to HXX. 1 1507x59122: Cry1F = 0.0038 g/kg seed (dw); Cry34Ab1 = 0.0633 g/kg seed (dw) and Cry35Ab1 = 0.0034 g/kg seed (dw) 

 1507: Cry1F = 0.0031 g/kg seed (dw) (Total Extractable Protein values from label were converted to a dry weight basis) 2 HXX: Cry1F = 0.0038 g/kg seed (dw); Cry34Ab1 = 0.0633 g/kg seed (dw) and Cry35Ab1 = 0.0034 g/kg seed (dw 

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G4. Details of the Proposed EUP Program 

Crop: Corn (Zea mays L.)

Target Pests: 

Agrotis ipsilon (Black cutworm) 

Helicoverpa zea (Corn earworm or Cotton bollworm) 

Ostrinia nubilalis (European corn borer) 

Spodoptera frugiperda (Fall armyworm) 

Diatraea grandiosella (Southwestern corn borer) 

Striacosta albicosta (Western bean cutworm) 

Elasmopalpus lignosellus (Lesser corn stalk borer) 

Diatrae crambidoides (Southern corn stalk borer) 

Diatrea saccharalis (Sugarcane borer) 

Diabrotica virgifera virgifera (Western corn rootworm) 

Diabrotica barberi (Northern corn rootworm) 

Diagrotica virgifera zeae (Mexican corn rootworm) 

Major Geographic Areas: Eleven states, representing diverse geographic regions primarily in the 

Corn Belt and other areas where there is potential crop injury from corn rootworm. 

Sites: Pioneer research centers, private cooperators, and privately owned farms. EUP experiments 

will not be located at EPA registered pesticide-producing establishments. All EUP experiments will be 

planted on existing agricultural land.

Period of Use: March 1, 2010 through May 31, 2011. 

G5. Universal Protocol Procedures 

The following procedures will be used for all EUP Experiments. 

Shipping and Labeling 

• Each shipment containing EUP Test Material must contain a copy of the EUP Master Label. 

• Seed will be shipped in seed bags or plastic bulk containers (“Proboxes”). 

• Seed bags are typically destroyed by the grower after planting. Proboxes are returned to Pioneer, 

cleaned, rinsed and re-used. 

• Complete documentation will be kept of seed invoiced (if applicable) and any returns. In addition, the 

following documents will be retained: federal EUP approval letter, state EUP approval letter (if 

applicable), EUP Master Label, Section G, and Grower Agreement (refuge compliance) if applicable. 

Schedule 

• Planting of experiments will generally take place from early March through the month of June. 

Observations and harvesting generally occur after planting through August to November. 

Submission of Cooperator Names 

• As per 40 CFR 172.4(b)(v), the names and street addresses of all cooperators will be submitted 

as soon as possible. Because of the large number of expected cooperators for this EUP and the 

possibility of cooperators dropping out or changing at the last minute, cooperator names may not 

be available prior to planting. All cooperator names will be submitted to EPA by August 1, 2010. 

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• Prior to August 1, 2010, if federal, regional or state inspectors wish to conduct an EUP inspection, 

they should request a current list of cooperators in their state or region from Tracy Rood, Sr. 

Registration Manager for North America at Pioneer. A current and accurate list will be provided 

within one working day. 

o E-mail: tracy.rood@pioneer.com 

o Office phone: 515-270-4036 

o Mobile phone: 515-229-8923 

(This information is also stated on the Master Label.) 

Planting 

• Seed will be planted by machine. 

• No special cleaning of planter is required before or after planting OAM1 or OAMRW blends or any 

other seed planted under this EUP. 

Marking of EUP experiments 

• Planting locations will be identified with stakes or, if equipment available, GPS coordinates. 

Isolation 

• No isolation is required because the events planted under this EUP are all registered by EPA 

and deregulated by USDA. In addition, both the Cry1F and Cry34/35 PIPs have full tolerance 

exemptions. 

Harvest Procedures 

• Vegetative material or grain will be harvested by machine. 

• Cleaning of harvest equipment is not required before or after harvesting OAM1 or OAMRW blends 

or any other seed planted under this EUP. 

Final disposition of harvested grain and vegetative material 

• Grain and vegetative material harvested under this EUP may enter food/feed streams through 

normal commercial channels. 

Post-Trial Monitoring 

• None required. 

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1. Grower Evaluation / Refuge Deployment Protocol

A. General Objective 

Validate agronomic performance and grower perception of blended refuge product concepts. 

• Cooperators will receive 1-2 pallets containing ~12-30 bags of corn seed each. Each pallet 

will include one or more of the following : 

o 1507x59122 corn blended with 1507 corn (5% blend) 

o 1507x59122 corn blended with 1507 corn (10% blend) 

o 59122 corn blended with non-Bt corn (5% blend) 

o 59122 corn blended with non-Bt corn (10% blend) 

• ~400-800 total locations 

• ~30-80 acres per location 

• ~10-15 hybrid genetic backgrounds across the geographies 

Note: A bag of seed plants approximately 2.7 acres. 

Cooperators will be encouraged to plant some non-Bt acres of similar genetics (outside the scope 

of the EUP) so they can evaluate the insect efficacy of blended refuge products compared to nonBt products. The non-Bt acres may be managed for pest control however the cooperator wishes: 

granular insecticide, liquid insecticide, and/or insecticide seed treatment. 

B. General Description of Data Collected 

• Some cooperators may compare the efficacy and agronomic performance of a 5% blend vs. 10% 

blend. Alternatively, some cooperators may compare the efficacy of 1507x59122 corn blended 

with 1507 corn as compared to 59122 corn blended with non-Bt corn against lepidopteran pests. 

• Some cooperators may evaluate 59122 corn blended with non-Bt corn as a block or strip 

lepidopteran refuge for 1507x59122 corn blended with 1507 corn. 

Pioneer will use a third-party firm to collect data from a subset of the growers at one or more times 

during the growing season. Because the survey has not been written yet, questions and data 

being collected are not available at this time. However, in general, growers will be surveyed about 

their expectations, perceptions, and experience with blended corn rootworm refuge product 

concepts. Categorical, analyzable data will likely be collected on various endpoints including: 

• Plantability 

• Plant growth and development 

• Lepidopteran insect damage in the 59122 blended with non-Bt corn acres 

• Lodging 

• Harvestability 

• Yield 

• Overall convenience relative to current refuge management options 

• Grain characteristics (quality and consistency) across blends at physiological maturity 

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2 Research Trial Protocol

A. General Objective 

Evaluate agronomic performance of 5% and 10% blended refuge products with various insecticide 

seed treatments across a wide range of geographies (up to 7 states across the Corn Belt) and 

diverse conditions. 

• ~25-30 locations 

• ~5-10 acres total per location 

• Treatments 

o 0% HXX 

o 5% 1507x59122 corn blended with 1507 corn 

o 10% 1507x59122 corn blended with 1507 corn 

o 0% HXRW 

o 5% 59122 corn blended with non-Bt corn 

o 10% 59122 corn blended with non-Bt 

o Untreated control to evaluate site pressure 

• The 5% and 10% blended refuge products may be evaluated different with insecticide seed 

treatments on refuge seeds (for example, Cruiser 250, Cruiser 500 or Poncho 1250) 

Roots will be dug at each location to ascertain general site pressure as well as specific damage to 

blended refuge plants with different insecticide seed treatments. 

B General Description of Data Collected 

• Early insect observations 

• Early stand count 

• Site pressure for corn rootworm (from untreated control) 

• Cluster sampling of blended refuge plants to evaluate insecticide seed treatments from blend 

treatments 

• Mid- to late-season insect observations 

• Lodging 

• Harvestability 

• Yield 

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CONFIDENTIAL ATTACHMENT to 

PHI-2009-118 

{CBI Cross-Reference Number 1} 

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