<PAGE>
                                                                    EXHIBIT 31.1
                                  CERTIFICATION

I, Richard J. DePiano, certify that:

1.   I have reviewed this annual report on Form 10-K/A 1A of Escalon Medical
     Corp. for the fiscal year ended June 30, 2007;

2.   Based on my knowledge, this report does not contain any untrue statement of
     a material fact or omit to state a material fact necessary to make the
     statements made, in light of the circumstances under which the statements
     were made, not misleading with respect to the periods covered by this
     report;

3.   Based on my knowledge, the financial statements, and other financial
     information included in this report, fairly present in all material
     respects the financial condition, results of operations and cash flows of
     the small business issuer as of, and for, the periods presented in this
     report.

4.   The small business issuer's other certifying officer and I are responsible
     for establishing and maintaining disclosure controls and procedures (as
     defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) for the small
     business issuer and have:

a.   Designed such disclosure controls and procedures, or caused such disclosure
     controls and procedures to be designed under our supervision, to ensure
     that material information relating to the small business issuer, including
     its consolidated subsidiaries, is made known to us by others within those
     entities, particularly during the period in which this report is being
     prepared;

b.   Evaluated the effectiveness of the small business issuer's disclosure
     controls and procedures presented in this report our conclusions about the
     effectiveness of the disclosure controls and procedures, as of the end of
     the period covered by this report based on such evaluation; and

c.   Disclosed in this report any change in the small business issuer's internal
     control over financial reporting that occurred during the small business
     issuer's most recent fiscal quarter (the small business issuer fourth
     fiscal quarter in the case of an annual report) that has materially
     affected, or is reasonably likely to materially affect, the small business
     issuer's internal control over financial reporting; and

5.   The small business issuer's other certifying officer(s) and I have
     disclosed, based on our most recent evaluation of internal control over
     financial reporting, to the small business issuer's auditors and the Audit
     Committee of the Small Business Issuer's Board of Directors (or persons
     performing the equivalent function):

a.   All significant deficiencies and material weaknesses in the design or
     operation of internal control over financial reporting which are reasonably
     likely to adversely affect the Small Business Issuer's ability to record,
     process, summarize and report financial information; and

b.   Any fraud, whether or not material, that involves management or other
     employees who have a significant role in the small business issuer's
     internal controls over financial reporting.

Date:  October 29, 2007
                                                --------------------------------
                                                Richard J. DePiano
                                                Chairman and Chief
                                                Executive Officer
                                                (Principal Executive Officer)


                                       19