STUDY NO. 00-4205 

SPONSOR STUDY NO. 211-DIPE-1G 

GASOLINE DIPE VAPOR CONDENSATE 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION 

TOXICITY STUDY IN RATS 

Final Report

Submitted to: American Petroleum Institute 

 1220 L Street, Northwest 

 Washington, D.C. 20005-4070 

 Attn: Thomas M. Gray, M.S., D.A.B.T. 

 Date: 4 August 2011 

Page 1 of 636 
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STATEMENT OF COMPLIANCE

This study was conducted in compliance with the United States Environmental Protection 

Agency's Good Laboratory Practice Standards 79.60, CFR Vol. 59, No. 122, 27 June 

1994 with the following exception: 

It was the Sponsor’s responsibility to maintain the method of synthesis, fabrication, or 

derivation of the test fuel, and this was not completed at the time of the study conduct but 

has been completed since and is on file with the Sponsor. 

Gary M. Hoffman, B.A., D.A.B.T. Date

Study Director 

Thomas M. Gray, M.S., D.A.B.T. Date

Sponsor Representative 
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SIGNATURE PAGE

SCIENTISTS

The following Scientists were responsible for the overall conduct of this study. 

Departmental supervisory personnel are listed on the personnel page of this report 

(Appendix EE). 

Gary M. Hoffman, B.A., D.A.B.T. Date

Study Director

Kevin Keane, D.V.M., Ph.D. Date

Director of Pathology1

SCIENTIFIC REVIEW

The following Scientists have reviewed and approved this report. 

Robert M. Parker, Ph.D., D.A.B.T. Date

Director, Developmental and Reproductive Toxicology 

Sylvie J. Gosselin, D.V.M., Ph.D., Diplomate A.C.V.P. Date

Vice President, Safety Assessment

 

1

Katharine M. Whitney was the Study Pathologist for this study and for submission of the draft report and is 

no longer employed at the Testing Facility. Kevin Keane is assuming responsibility for finalization of the 

pathology evaluation of this report.
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QUALITY ASSURANCE STATEMENT

Listed below are the dates that this study was inspected by the Quality Assurance Unit of 

Huntingdon Life Sciences, East Millstone, New Jersey, and the dates that findings were 

reported to the Study Director and Management. This report reflects the raw data as far 

as can be reasonably established.

Type of Inspection

Date(s) of 

Inspection

Reported to

Study Director 

and Management

Pathology Associates Facility 

Inspection 

24 Apr 01 02 Nov 01 

GLP Protocol Review 01 Apr 02 01 Apr 02 

Exposure, Monitoring and Equipment 

Records

20 May 02 20 May 02 

Body and Feeder Weights 17 Jun 02 17 Jun 02 

Estrous Determination 09 Jul 02 09 Jul 02 

Litter Checks and Daily Viability 

Check

20 Aug 02 20 Aug 02 

Male Necropsy and Sperm 

Assessment

10 Sep 02 11 Sep 02 

Maternal & Pup Necropsy and 

Training Records 

17 Sep 02 17 Sep 02 

Final Analytical Report and Study 

Data

7-10, 20 and 21 Jan 

03 

21 Jan 03 

Final Report Draft and Study Data 09, 10, 13-17, 20-

24, 26, 27 Jan 03 

27 Jan 03 

Final Report Review 04-17 Feb 10 17 Feb 10 

Lesley Piccoli Date

Quality Assurance Auditor 
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Gasoline DIPE Vapor Condensate: A One-Generation

Whole-Body Inhalation Reproduction Toxicity Study in Rats 

SUMMARY

This study was designed to assess the potential reproductive toxicity of Gasoline DIPE 

Vapor Condensate when administered via whole-body inhalation exposures to Sprague 

Dawley rats (26/sex/group). The test substance was administered (using a nitrogen 

enriched air mixture) at target concentrations of 2000, 10000 and 20000 mg/m3 for 

6 hours/day, 7 days/week for 10 weeks before mating, for up to 2 weeks during mating, 

for 3 weeks during gestation and for 4 weeks during lactation prior to weaning. In 

addition, a control group (26/sex) received nitrogen enriched air only while in chamber. 

Exposure levels were determined using an infra-red spectrophotometer 4 times per 

chamber per day. Additionally, the test substance’s major components were assayed once 

per chamber per week. Particle size distribution measurements were also made once per 

chamber per week using a TSI Aerodynamic Particle Sizer. 

Viability checks were performed twice daily to check for mortality and signs of severe 

toxic or pharmacologic effects. Physical observations and body weight measurements 

were made twice pretest and at least weekly during the study. Feed consumption 

measurements were obtained beginning the week prior to treatment initiation and at least 

weekly during the study. After completion of ~ 16 weeks of exposures, all parental male 

animals (P0 generation) were sacrificed. All parental female animals (P0 generation) 

were sacrificed on Lactation Day 28. Selected organs were weighed and organ/body 

weight and organ/brain weight ratios calculated. Complete macroscopic postmortem 

examinations were performed on all parental animals. Histopathological evaluations of 

selected tissues were conducted on randomly selected parental animals.

Pups (F1 generation) were observed as soon as possible after delivery for their sex, the 

number of live and dead pups and pup abnormalities. Thereafter, litters were observed 

twice daily. On the 4th day after parturition, litters with more than ten pups were 

randomly culled to ten pups with sex distribution equalized when possible. Pups were 

given a gross physical examination, were weighed and were sexed frequently until 

weaning on the 28th day after parturition at which time they were sacrificed. A 

macroscopic postmortem evaluation and the weighing of selected organs were performed 

on randomly selected pups. 

The mean (± standard deviation) analytical exposure concentrations of Gasoline DIPE 

Vapor Condensate were determined to be 0 ± 0, 2034 ± 128, 10260 ± 621 and 

20230 ± 775 mg/m3 for the Air Control and the exposure groups, respectively. The 
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SUMMARY

analytically measured exposure levels of the airborne test substance were acceptably close 

to the targeted exposure levels. The measured and nominal concentrations varied 

somewhat (less than 3%), but acceptably, from the expected 1:1 ratio for this type of 

vapor exposure. Chamber environmental conditions averaged 25oC temperature and 46% 

relative humidity. Particle sizing results indicated that the atmospheres were essentially 

vapor only, as expected, since there was no substantial difference between the particulate 

levels in the test substance chambers and the Air Control chamber. Analysis of the major 

components in the neat test substance and the test atmospheres showed an acceptably 

close comparison between the neat test substance and the vaporized test substance. This 

data demonstrated that the test animals were exposed, as expected, to all of the major 

components of the test substance in their acceptably proper proportion. The data was 

consistent from week-to-week during the study indicating stability of the test substance 

and the atmosphere generation techniques. 

There was no effect of treatment on survival, clinical observations, body weight gain, 

feed consumption, estrous cycle (as measured by cycle length and number of estrous 

cycles), mating indices for the male rats and mating or fertility or gestation indices for the 

female rats. There were also no treatment-related differences in the other reproductive 

parameters up to the time of parturition including the percent of females completing 

delivery and the duration of gestation, when compared to the Air Control group. There 

were also no treatment-related differences in all parturition parameters including the total 

number of pups delivered, the number of pups dying, the viability (4 day survival) and 

lactation (21 day survival) indices, the number of implantation sites per litter, the pup sex 

ratio and the number of live pups/litter, when compared to the Air Control group. Mean 

percent motility, epididymal sperm count, homogenization-resistant testicular spermatid 

count, sperm morphology and caudal epididymis weight were not affected by treatment 

with the test substance at an exposure level of 20000 mg/m3. 

There were no exposure-related differences in terminal estrous evaluations in the test 

substance exposed animals compared to the Air Control animals. Scattered macroscopic 

postmortem observations such as lung discoloration were noted in a treatment-related 

pattern, but in absence of any effects on lung weights or histopathology, were not 

considered adverse findings. Exposure-related effects on organ weights included 

statistically significant increases in liver weights (absolute and/or relative to body weight 

or brain weight) at the 2 higher exposure levels in the males. Exposure-related effects on 

organ weights also included statistically significant increases in kidney weights (absolute 

and/or relative to body and brain weight) at the 2 higher exposure levels in the males. 

These differences for the males were consistent with the hydrocarbon nephropathy 
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SUMMARY

findings at these exposure levels in the 13-week inhalation study with this same test 

substance. This species- and gender-specific change has been well documented in male 

rats exposed to a variety of hydrocarbon compounds and is not considered relevant to 

humans. No test substance related microscopic changes were noted in male and female 

reproductive organs or other protocol-specified tissues in this study.

The pups were unremarkable for clinical observations and body weights and weight gains 

during the lactation period. There were no exposure-related differences in macroscopic 

postmortem evaluations and organ weights in the pups feeding from test substance 

exposed animals compared to the pups feeding from Air Control animals.

In conclusion, exposure of rats to 2000, 10000 and 20000 mg/m3 of vapor of Gasoline 

DIPE Vapor Condensate resulted in no effects on the exposed parental animals prior to 

their termination. However, increases in liver weights in parental male animals exposed 

to 10000 and 20000 mg/m3 of vapor were seen. Increases in kidney weights in parental 

male animals exposed to the two higher exposure levels of vapor were consistent with 

hydrocarbon nephropathy seen in similar animals exposed for 13 weeks to this test 

substance. However, this finding has been generally accepted (US EPA 1991. Alpha 2 

microglobulin: association with chemically induced renal toxicity and neoplasia in male 

rat. In Risk Assessment Forum, p.85. US Govt. Printing Office, Washington, DC.) not to 

be relevant to human risk assessment. Therefore, a no observable effect level (NOEL) of 

2000 mg/m3 was determined. There was no effect at any of the exposure levels on 

reproductive performance in the study, including mating, fertility, parturition, lactation 

and offspring survival and development for 28 days after birth. Therefore, with respect to 

reproductive performance, a no observable effect level (NOEL) of 20000 mg/m3 was 

determined.
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TABLE OF CONTENTS 

COVER PAGE .....................................................................................................................1 

STATEMENT OF COMPLIANCE .....................................................................................2 

SIGNATURE PAGE ...........................................................................................................3 

QUALITY ASSURANCE STATEMENT ..........................................................................4 

SUMMARY .........................................................................................................................5 

TABLE OF CONTENTS .....................................................................................................8 

1. INTRODUCTION ................................................................................................11 

2. MATERIALS AND METHODS

2.1. Study Management ....................................................................................11 

2.2. Study Dates ................................................................................................11 

2.3. Experimental Outline .................................................................................13 

2.4. Test Substance ...........................................................................................14 

2.5. Test Animals ..............................................................................................15 

2.6. Animal Assignment ...................................................................................16 

2.7. Animal Identification .................................................................................17 

2.8. Veterinary Care ..........................................................................................17 

2.9. Animal Husbandry During Non-Exposure Periods ...................................17 

2.10. Test Substance Administration and Chamber Operation ...........................20 

2.11. Experimental Evaluations ..........................................................................20 

2.12. Mating, Gestation and Lactation Procedures .............................................21 

2.13. Postmortem ................................................................................................23 

2.14. Sperm Count, Motility and Morphology Assessments ..............................27 

2.15. Statistical Analysis .....................................................................................28 

2.16. Data Storage ...............................................................................................30 

2.17. Regulatory References ...............................................................................30 

2.18. Protocol Deviations ....................................................................................31 

3. RESULTS AND DISCUSSION

3.1. Chamber Monitoring ..................................................................................33 

3.2. Parental Data ..............................................................................................35 

3.3. Pup Data .....................................................................................................38 

3.4. Postmortem Data ........................................................................................39 

4. CONCLUSION ....................................................................................................41 
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TABLE OF CONTENTS 

REFERENCES ..................................................................................................................42

CALCULATIONS .............................................................................................................43 

GENERAL PREFACE ......................................................................................................44 

TABLES

1. Summary of Survival and Pregnancy ........................................................................... 45 

2. Summary of Clinical Observations - (Premating/Mating/Postmating) ........................ 46 

3. Summary of Clinical Observations During Gestation .................................................. 52 

4. Summary of Maternal Clinical Observations During Lactation .................................. 53 

5. Mean Body Weight Values – (Premating/Mating/Postmating) ................................... 54 

6. Mean Body Weight Gain - (Premating /Mating/Postmating) ...................................... 59 

7. Summary of Gestation Body Weights .......................................................................... 64 

8. Summary of Gestation Body Weight Gain ................................................................... 65 

9. Summary of Maternal Lactation Body Weights ........................................................... 66 

10. Summary of Maternal Lactation Body Weight Gain ................................................... 67 

11. Mean Feed Consumption (grams/kg/day) - (Premating/Postmating) ......................... 68 

12. Summary of Gestation Feed Consumption (grams/kg/day) ........................................ 72 

13. Summary of Lactation Feed Consumption (grams/kg/day) ........................................ 73 

14. Summary of Estrous Stages ........................................................................................... 74 

15. Summary of Cohabitation Data ..................................................................................... 75 

16. Summary of Delivery and Litter Data .......................................................................... 76 

17. Summary of Pup Clinical Observations During Lactation ........................................... 80 

18. Summary of Mean Pup Body Weights and Body Weight Gain ................................... 81 

19. Summary of Parental Necropsy Observations .............................................................. 87 

20. Summary of Pup Necropsy Observations ..................................................................... 92 

21. Summary of Parental Organ Weights ........................................................................... 99 

22. Summary of Pup Organ Weights ................................................................................ 112 

23. Lesion Incidence Summary with Expanded Severity Levels ..................................... 116 

APPENDICES

A. Inhalation Report ......................................................................................................... 121

B. Analytical Report ......................................................................................................... 173

C. Individual Animal Termination History ..................................................................... 203 

D. Individual Clinical Observations - (Premating/Mating/Postmating) ......................... 211 

E. Individual Clinical Observations During Gestation ................................................... 233 

F. Individual Maternal Clinical Observations During Lactation .................................... 241 

G. Individual Body Weights - (Premating/Mating/Postmating) ..................................... 253 

H. Individual Body Weight Gain - (Premating/Mating/Postmating) .............................. 270 
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TABLE OF CONTENTS 

I. Individual Gestation Body Weights ............................................................................ 283 

J. Individual Gestation Body Weight Gain .................................................................... 288 

K. Individual Maternal Lactation Body Weights ............................................................ 293 

L. Individual Maternal Lactation Body Weight Gain ..................................................... 298 

M. Individual Feed Consumption (grams/kg/day) - (Premating/Postmating) ................. 303 

N. Individual Gestation Feed Consumption (grams/kg/day) .......................................... 312 

O. Individual Maternal Lactation Feed Consumption (grams/kg/day) ........................... 317 

P. Individual Estrous Cycle and Mating Data ................................................................. 322 

Q. Individual Mating Assignments .................................................................................. 330 

R. Individual Delivery and Litter Data ............................................................................ 334 

S. Individual Pup Sex and Status During Lactation ........................................................ 338 

T. Individual Pup Clinical Observations During Lactation ............................................ 342

U. Individual Pup Body Weights ..................................................................................... 382 

V. Individual Parental Necropsy Observations ............................................................... 406

W. Individual Pup Necropsy Observations ...................................................................... 429

X. Individual Parental Organ Weights ............................................................................. 462

Y. Individual Pup Organ Weights .................................................................................... 491

Z. Individual Animal Gross and Microscopic Observations .......................................... 508 

AA. Sperm Analysis Report ...........................................................................................550 

BB. Certificates of Analysis – (Feed, Water and Bedding) ...........................................564 

CC. Protocol and Protocol Amendments .......................................................................598 

DD. Historical Control Data ...........................................................................................630 

EE. Testing Facility Personnel ........................................................................................... 635 

FF. Report Amendments .................................................................................................... 636 
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1. INTRODUCTION

This reproductive study was designed to provide general information concerning 

the effects of Gasoline DIPE Vapor Condensate on the integrity and performance 

of the male and female reproductive systems, including gonadal function, the 

estrous cycle, mating behavior, conception, gestation, parturition and lactation, 

and to provide information about the effects of the test substance on neonatal 

morbidity and mortality, and data on prenatal and postnatal developmental 

toxicity.

2. MATERIALS AND METHODS

2.1. STUDY MANAGEMENT

2.1.1. SPONSOR

American Petroleum Institute (API)

1220 L Street, Northwest 

Washington, DC 20005-4070 

2.1.2. SPONSOR REPRESENTATIVE

Thomas M. Gray, M.S., D.A.B.T. 

2.1.3. TESTING FACILITY

Huntingdon Life Sciences 

P.O. Box 2360 

Mettlers Road

East Millstone, New Jersey 08875-2360 

2.1.4. STUDY DIRECTOR

Gary M. Hoffman, B.A., D.A.B.T. 

2.2. STUDY DATES

2.2.1. STUDY INITIATION

6 May 2002 (Date Study Director signed the Protocol) 
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2.2.2. DATE OF ANIMAL RECEIPT

6 May 2002 

2.2.3. EXPOSURE INITIATION

20 May 2002 (Experimental Start Date) 

2.2.4. MATING INITIATION

28 July 2002 

2.2.5. EXPOSURE TERMINATION

23 September 2002 

2.2.6. TERMINAL SACRIFICE 

P0 Males: 10 – 11 September 2002 

P0 Females: 23 August 2002 – 24 September 2002 

2.2.7. EXPERIMENTAL TERMINATION DATE

4 August 2011 (Date of last data collection = Date Pathologist 

signed the Final Report) 

2.2.8. STUDY COMPLETION

4 August 2011 (Date Study Director signed the Final Report) 
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2.3. EXPERIMENTAL OUTLINE

Group

Group 

Designation

Exposure 

Levels 

(mg/m3)

 a

Number of Animals 

Mated Adults

Microscopic 

Pathology Adult 

Generationsb Macroscopic Postmortem 

P0 P0 Examinations-Pups

M F M F F1

I Control 0 (air only) 26 26 10 10 3/sex/litter

II Low 2,000 26 26 0 0 3/sex/litter

III Middle 10,000 26 26 0 0 3/sex/litter

IV High 20,000 26 26 10 10 3/sex/litter a Exposures daily (7 days/week) for 6 hours per day. 

b

Histologic examinations were performed on reproductive and select tissues for the control and high-exposure 

animals.

M = Male; F = Female; The first day of exposure was Day 0.
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2.4. TEST SUBSTANCE 

Gasoline DIPE Vapor Condensate 

2.4.1. SUPPLIER

Chevron Texaco Energy Research and Technology Company 

100 Chevron Way 

Richmond, CA 94802 

2.4.2. LOT NUMBER

API-01-06 

2.4.3. PURITY/ANALYTICAL CONCENTRATION

100% Gasoline DIPE Vapor Condensate 

2.4.4. DESCRIPTION

Colorless Liquid 

2.4.5. DATES RECEIVED

7 December 2001 & 5 August 2002 

2.4.6. EXPIRATION DATE

Not available; stable per MSDS.

2.4.7. ANALYSIS

Documentation of the identity, strength, purity, composition, 

stability, and method of synthesis, fabrication, and/or derivation of 

the test article and the maintenance of these records was the 

responsibility of the Sponsor. The Sponsor conducted a purity 

analysis of the test substance by GC prior to the start of this study. 

2.4.8. STORAGE

Ambient conditions in an outdoor solvent shed or in an indoor 

laboratory. 
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2.4.9. DISPENSING

The test substance was received from Chevron Texaco Energy 

Research and Technology Company in 100 gallon cylinders. Since 

only 5-gallon cylinders were practical to be used for exposure 

operations, the test substance was dispensed, as needed, at the 

Testing Facility from the 100-gallon cylinders into 5-gallon 

cylinders using nitrogen pressurization. 

2.4.10. ARCHIVAL SAMPLE

An archival sample from test substance is stored in the Archives of 

the Testing Facility under conditions specified for test substance 

storage.

2.4.11. DISPOSITION

The unused portion of the test substance as well as any empty test 

substance containers was returned to the Sponsor on 11 January 

2007. Empty test substance containers were returned on an as 

needed basis. The Sponsor was responsible for tracking their 

disposition. 

2.5. TEST ANIMALS 

Albino Rats (Outbred) VAF/Plus

2.5.1. SPECIES

Sprague-Dawley derived CD

[Crl: CD IGS BR]

2.5.2. SUPPLIER

Charles River Laboratories

Kingston, New York 12484 

2.5.3. JUSTIFICATION FOR TEST ANIMAL SELECTION

The rat was used as a surrogate for humans to detect adverse 

reproductive effects and is a species in which known reproductive 

toxicants have been detected. The rat is a rodent animal model 

commonly utilized in reproduction studies and is recommended in 
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the referenced guidelines. In addition, a historical control database 

with this strain of animal and supplier facility is available for 

comparative evaluation. 

2.5.4. NUMBER OF ANIMALS

Received:

220 total (110 males, 110 females) 

Placed on test:

208 total (104 males, 104 females) 

Females were nulliparous and non-pregnant. 

2.5.5. AGE AT RECEIPT

Approximately 28 days. 

2.5.6. AGE AT INITIATION OF EXPOSURES

Approximately 42 days. 

2.5.7. WEIGHT AT INITIATION OF EXPOSURES (GRAMS)

Mean Range

Male: 213 186 - 245 

Female: 153 130 - 179 

Individual weights of animals placed on test were within ±20% of 

the mean weight for each sex.

2.5.8. ACCLIMATION PERIOD

Animals were acclimated for 14 days. All animals were examined 

during the acclimation period to confirm suitability for study.

2.6. ANIMAL ASSIGNMENT

More animals than required for the study were purchased and acclimated. 

Animals considered suitable for the study on the basis of pretest body 

weight data and pretest physical examinations, were randomly assigned, by 

sex, to control or treated group, in an attempt to equalize mean group body 

weights.
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2.7. ANIMAL IDENTIFICATION

Each rat was assigned a temporary identification number upon receipt. 

After selection for study (P0 generation), each rat was identified with a 

metal ear tag bearing its assigned animal number. The assigned animal 

number plus the study number comprised the unique animal number for 

each animal. If the tag was lost, it was replaced. In addition, each cage 

was provided with a cage card that was color-coded for exposure level 

identification and contained the study number and animal number 

information. 

2.8. VETERINARY CARE

Animals were monitored by the technical staff for any conditions requiring 

possible veterinary care and treated as necessary. Miscellaneous, non-test 

article-related veterinarian evaluations and treatments (as necessary) for 

individual animals were reviewed by the study director and are 

documented in the study file. 

2.9. ANIMAL HUSBANDRY DURING NON-EXPOSURE PERIODS

2.9.1. FACILITIES MANAGEMENT/ANIMAL HUSBANDRY

Currently acceptable practices of good animal husbandry were 

followed e.g., Guide for the Care and Use of Laboratory Animals; 

National Academy Press, 1996. Huntingdon Life Sciences, East 

Millstone, New Jersey is fully accredited by the Association for 

Assessment and Accreditation of Laboratory Animal Care 

International (AAALAC).

2.9.2. HOUSING

Animals were housed in suspended, stainless steel cages with wire 

mesh fronts and floors. Animals were doubly housed during the 

initial week of acclimation and individually housed thereafter, 

except as follows. 

Mating: One male and one female were co-housed continuously 

(except during treatment) for 14 days, or until mating occurred. 

Lactation: Dam and litter were housed together in a solid plastic 

“shoebox” cage until weaning.
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2.9.3. FEED

Certified Rodent Diet, No. 5002; (Meal) (PMI Nutrition 

International, St. Louis, Missouri) was available without restriction 

except during exposures. 

2.9.4. FEED ANALYSIS

Analysis of each feed lot used during this study was performed by 

the manufacturer. Results can be found in Appendix BB. There 

were no known contaminants in the feed, which were expected to 

interfere with the results of this study.

2.9.5. WATER

Water (Elizabethtown Water Company, Westfield, New Jersey) 

was available without restriction, except during exposures, via an 

automated watering system.

2.9.6. WATER ANALYSIS

Water analyses are conducted by Elizabethtown Water Company, 

Westfield, New Jersey (Raritan-East Millstone Plant) to ensure that 

water meets standards specified under the EPA Federal Safe 

Drinking Water Act Regulations (40 CFR Part 141). In addition, 

water samples are collected biannually from representative rooms 

in the Testing Facility; chemical and microbiological water 

analyses are conducted on these samples by a subcontract 

laboratory. Results of all water analyses can be found in Appendix 

BB. There were no known contaminants in the water that were 

expected to interfere with the results of this study.

2.9.7. BEDDING SUBSTANCE

Ground corncob bedding (Bed-O’-Cobs 1⁄4 inch irradiated, The 

Andersons, Maumee, OH was provided for each mated female on 

Day 18 of gestation. Fresh bedding was provided at least weekly 

and as needed during the lactation period (litters were weaned on 

Day 28 of lactation). 
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2.9.8. BEDDING ANALYSIS

Analyses for each batch of bedding used on study can be found in 

Appendix BB. There were no known contaminants in the bedding 

that were expected to interfere with the results of this study.

2.9.9. ENVIRONMENTAL CONDITIONS

Light/Dark Cycle 

A twelve-hour light/dark cycle controlled by an automatic timer 

was provided. 

Temperature

Temperature was monitored in accordance with Testing Facility 

SOPs and maintained within the specified range to the maximum 

extent possible. Excursions outside the specified range were 

considered not to have affected the integrity of the study. 

Desired: 19 to 25°C 

Actual: 15.6 to 31.7°C 

Average: 18.5 to 26.4°C 

Relative Humidity

Relative humidity was monitored in accordance with Testing 

Facility SOPs and maintained within the specified range to the 

maximum extent possible. Excursions outside the specified range 

were considered not to have affected the integrity of the study. 

Desired: 30 to 70% 

Actual: 15.7 to 100.0% 

Average: 22.0 to 75.5% 

Air Changes

Air Changes were monitored in accordance with Testing Facility 

SOPs and maintained within the specified range to the maximum 

extent possible. 

Desired: 10 - 15 per hour 

Actual: 14.6 – 14.7 per hour 
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2.10. TEST SUBSTANCE ADMINISTRATION AND CHAMBER 

OPERATION

Inhalation (whole-body exposures). 

Details of test article administration and chamber operation information 

are presented in the Inhalation Report (Appendix A). 

2.11. EXPERIMENTAL EVALUATIONS

2.11.1. VIABILITY EXAMINATION (CAGESIDE)

Observations for mortality and signs of severe toxic or 

pharmacological effects were made at least twice daily (morning 

and afternoon). 

2.11.2. DETAILED PHYSICAL EXAMINATIONS

The animals were observed as a group at least once during each 

exposure. 

In addition, each parental animal was removed from its cage and 

examined twice pretest, and weekly thereafter during the study 

period. Females were then continuously observed weekly until 

there was evidence of mating. Once mated, females were observed 

on Gestation Days 0, 7, 14, and 20 and on Lactation Days 1, 4, 7, 

14, 21, and 28. Females that showed evidence of mating but did 

not deliver were observed on Gestation Days 0, 4, 7, 14, and 20 

and were euthanized on Gestation Day 25. Females without 

evidence of mating were continuously observed weekly until 

euthanized on the last possible Gestation Day 25. Examinations 

included, but were not limited to, observations of general 

condition, skin and fur, eyes, nose, oral cavity, abdomen and 

external genitalia, as well as, evaluations of respiration and 

palpation for tissue masses. During the exposure period, physical 

examinations were performed post-exposure except they were 

performed pre-exposure during the gestation and lactation periods. 

2.11.3. BODY WEIGHT

Each parental animal was removed from its cage and weighed 

twice pretest, and weekly thereafter during the study period 
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(including the mating period). Once mated, females were weighed 

on Gestation Days 0, 4, 7, 14, and 20 and on Lactation Days 1, 4, 7, 

14, 21, and 28. Females that did not show evidence of mating 

and/or did not deliver pups were weighed weekly until euthanasia. 

2.11.4. FEED CONSUMPTION

Feed consumption was measured once pretest and weekly 

thereafter during the study period. Once mated, female feed 

consumption was measured on Gestation Days 0, 4, 7, 14, and 20 

and on Lactation Days 1, 4, 7, 14, 21, and 28 until euthanasia. 

Females without evidence of mating and/or that did not deliver 

pups had consumption measured weekly until euthanasia. Feed 

consumption was not measured for any animal during the mating 

period when males and females were co-housed. 

Calculation 

Feed Consumption (g/kg/day) = 

grams of food consumed ÷ # days 

body weight (kg)a

a

The average of the current and previous weight was used. 

2.12. MATING, GESTATION AND LACTATION PROCEDURES

2.12.1. ESTROUS CYCLING

Daily vaginal smears were taken at approximately the same time 

each day and the stage of the estrous cycle was determined for each 

P0 female beginning on Test Day 49, three weeks prior to 

cohabitation, and continued until there was evidence of mating or 

until the 14-day mating period ended on Test Day 83. 

2.12.2. MATING PROCEDURE

Following a 10-week pre-mating treatment period, one male and 

one female from the same exposure group were caged together 

until evidence of mating was observed (a copulation plug in the 

vagina and/or microscopic observation of sperm in the vaginal 

smear) or 14 consecutive days had elapsed. During cohabitation 
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period exposures, males and females were not paired together. The 

day evidence of mating was observed was defined as Day 0 of 

gestation. 

2.12.3. PATURITION AND LACTATION

On Day 18 of gestation, several days prior to expected parturition, 

each female was transferred to a solid plastic “shoe-box” cage. 

Bedding substance was provided and changed at least weekly as 

needed. Examinations for signs of parturition were made twice 

daily (morning and afternoon). The day on which parturition was 

first observed was defined as Day 0 of lactation. Females that 

exhibited no evidence of mating were transferred to plastic “shoebox” cages when the first animals mated reached their Day 18 of 

gestation.

2.12.4. LITTER EVALUATIONS (F1) 

Observations 

F1 litters were observed as soon as possible after delivery for the

number of live and dead pups, sex of the pups and pup 

abnormalities. The sex was determined on the day of delivery 

completion for all pups by inspection of the anogenital distance. 

All pups were uniquely identified within the litter by toe tattoo on 

the day of delivery completion. Thereafter, litters were observed 

twice daily (morning and afternoon) for the presence of dead pups. 

These dead pups were examined to the extent possible for defects 

and/or cause of death (lung floatation test to distinguish stillborn 

and found dead pups) and preserved in neutral, phosphate-buffered 

10% formalin. Autolyzed remains found on the day of parturition 

were presumed to be stillborn. Litter size was recorded daily from 

Lactation Day 1 until weaning on Lactation Day 28. 

Culling Pups

On Day 4 of lactation, each litter with more that 10 pups was 

culled to that number, with sex distribution equalized (five/sex) 

when possible. The pups were culled randomly, preferential 

culling of runts not being performed. 
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Physical Examinations 

Each F1 pup was given a macroscopic physical examination on the 

day of delivery completion and on Day 4, 7, 14, 21 and 28 of 

lactation.

Body Weight 

Individual F1 pup weights were recorded on Day 1, 4 (precull 

intervals), 7, 14, 21 and 28 of lactation (postcull intervals). 

Sexing

The sex of each individual pup was recorded on the day of delivery 

completion, then reconfirmed on Lactation Day 4, 7, 14, 21, and at 

weaning on Lactation Day 28. 

2.13. POSTMORTEM 

2.13.1. PARENTAL ANIMALS (P0) 

Necropsy Information 

Necropsy was performed on up to 26 animals/sex/group. All P0

males were euthanized after the last day that the F1 litters were 

delivered. All P0 females with litters were euthanized on Day 28 

of lactation. P0 females with no confirmed day of mating were 

euthanized on last possible Gestation Day 25. P0 females with 

confirmed days of mating that did not deliver were euthanized on 

Gestation Day 25. Animals were fasted overnight prior to 

necropsy. 

If a dam entirely lost her litter, she resumed exposures the next day 

(if prior to lactation day 5) or otherwise continued exposures. She 

was sacrificed on the initial day of weaning for the study (or later, 

as practical) and macroscopically examined and tissues retained 

but no organs were weighed. 

Method of Euthanasia 

Exsanguination following anesthesia with inhaled carbon dioxide. 
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Macroscopic Examination 

Complete macroscopic postmortem examinations were performed 

on all adult animals, and all abnormal observations were recorded. 

The necropsy of the parental animals included examination of the 

external surface and all orifices; the external surfaces of the brain 

and spinal cord; the organs and tissues of the cranial, thoracic, 

abdominal and pelvic cavities and neck; and the remainder of the 

carcass. Examination of all P0 females included a vaginal smear to 

determine the stage of the estrous cycle and a count of uterine 

implantation scars, if present.

Organ Weights 

Organs indicated in Table I (page 25) were weighed for all animals 

at the scheduled sacrifice intervals. Prior to weighing, the organs 

were carefully dissected and properly trimmed to remove adipose 

and other contiguous tissues in a uniform manner. Organs were 

weighed as soon as possible after dissection in order to avoid 

drying. Paired organs were weighed together (gonads were 

weighed separately). Organ weight data is presented as absolute 

values and relative to terminal body weight and brain weight. 

Organ weights were not recorded for an animal dying 

spontaneously or euthanized moribund. 

Tissues Preserved and Examined Histopathologically 

The tissues listed in Table I (page 25) were obtained at the 

scheduled sacrifice intervals and preserved for all animals. Slides 

of the indicated tissues were prepared and examined 

microscopically for 10 randomly selected animals per sex per 

group in the control and high exposure groups. Any abnormalities 

not noted during macroscopic examinations which were seen 

during histology processing were recorded. 

Histopathological examinations of the testes were conducted to 

identify potential treatment-related effects such as retained 

spermatids, missing germ cell layers or types, multinucleated giant 

cells, or sloughing of spermatogenic cells into the lumen. The 

examination of the intact epididymis was of a longitudinal section 

through the caput, corpus and cauda regions to permit detection of 
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lesions such as sperm granulomas, leukocytic infiltration 

(inflammation), aberrant cell types within the lumen, or the 

absence of clear cells in the cauda epididymal epithelium. 

Histopathological examination of the ovary included evaluation of 

five sections taken at least 100 μm apart from the inner third of 

each ovary permitting detection of depletion of the primordial 

follicle population and enumeration of the total number of 

primordial follicles for comparison with the ovaries from control 

animals. These examinations also confirmed the presence or 

absence of growing follicles and corpora lutea in comparison to 

control ovaries. 

TABLE I 

ORGAN NAME WEIGHED PRESERVED

EXAMINED

MICROSCOPICALLY

(Groups I and IV)

adrenal glands X X X 

brain (medulla, pons, cerebrum and 

cerebellum) X X 

epididymides (total and caudal) X Xa Xa 

kidneys X X 

liver X X 

lungs (with mainstem bronchi) X X X 

ovaries X X X 

pituitary gland X X X 

prostate gland X X X 

seminal vesicles with coagulating glands X X X 

spleen X X 

testes X Xa Xa 

uterus (body/horns) with cervix and oviducts X X X 

vagina X X 

tissues with macroscopic findings including 

tissue masses X X 

a

Right testis and epididymis only.

Preservatives

All tissues - 10% neutral buffered formalin (NBF).
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Testes and epididymides were placed in Modified Davidson’s 

solution initially and then retained in 10% formalin. Lungs were 

infused with formalin prior to their immersion into a larger volume 

of the same fixative.

Processing

After fixation, the tissues and organs from all animals were 

routinely processed, embedded in paraffin, cut at a microtome 

setting of 4-7 microns, mounted on glass slides and stained with 

hematoxylin and eosin. 

2.13.2. F1 PUPS 

Method of Euthanasia

Culled F1 pups, or those sacrificed prior to the day of weaning, 

were euthanized by IP injection of sodium pentobarbital. 

Weanlings were euthanized by exsanguination after anesthesia with

carbon dioxide. 

Dead and Culled Pups 

F1 pups found dead at birth were identified (lung floatation test) as 

stillborn or live-born but found dead. F1 pups found dead during 

the lactation period were examined to the maximum extent 

possible for defects and/or the cause of death, and for the presence 

or absence of milk in the stomach. Dead pups were preserved 

intact in 10% NBF. Partially cannibalized pups were examined to 

the maximum extent possible and discarded or preserved intact in 

10% NBF. Culled F1 pups were examined for external 

abnormalities. If unremarkable, these pups were then euthanized 

and discarded. Culled F1 pups with external abnormalities were 

preserved intact in 10% NBF. 

Macroscopic Examination and Tissues Preserved

Macroscopic examinations were performed on up to 3 randomly 

selected pups/sex/litter on Day 28 of lactation. Examinations 

included observations of any structural abnormalities or 

pathological changes. Special attention was given to the organs of 
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the reproductive system, brain, liver, kidneys, pituitary, adrenal 

gland, spleen, heart and thymus gland. 

All remaining F1 pups were examined for external abnormalities 

and euthanized. Pups with external abnormalities were preserved 

intact in 10% NBF. Pups with no external abnormalities were 

discarded. No further macroscopic postmortem examinations were 

performed.

Organ Weights 

The following organs were weighed from one randomly selected 

pup/sex/litter sacrificed on Day 28 of lactation:

brain

spleen

thymus gland 

Organ weight data is presented as absolute values and relative to 

terminal body weight and brain weight. 

2.14. SPERM COUNT, MOTILITY AND MORPHOLOGY 

ASSESSMENTS

The following sperm evaluations were conducted for all control and high 

exposure P0 males (samples and images were collected but not evaluated 

for all other males): 1) motility; 2) a count of homogenization-resistant 

testicular spermatids; 3) a count of caudal epididymal sperm; and 4) sperm 

morphology (cauda epididymis). Evaluations were performed by 

Pathology Associates International, Frederick, MD (PAI)as follows: 

The right testis and right epididymis from each animal were removed 

intact, weighed (testes weighed together and also separately) and 

preserved. 

The left epididymis was removed intact, weighed, and frozen on dry ice 

for transport to PAI. The epididymides were stored frozen at -70°C until 

evaluation for caudal sperm count. Each epididymis was thawed and the 

caudal portion removed and weighed. A homogenized sample of the 

caudal epididymis was stained and then examined using the Hamilton 

Thorne IVOS sperm analyzer. For each stained preparation, 20 fields were 
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counted. The total number of sperm in the caudal epididymis was 

calculated and adjusted for the caudal epididymal weight. Additionally, 

for each male, two sperm morphology slides were prepared, stained with 

Eosin and evaluated for morphological development. 

The left vas deferens was excised and placed in a prewarmed solution of 

phosphate buffered saline and 1% Bovine Serum Albumin. After a 

minimum three minute “swimout” period, a sample was placed in a 

Hamilton Thorne IVOS sperm analyzer. Five fields were selected and 

stored as digital images. The images were analyzed for percent motility 

and transferred to optical media for permanent storage.

The left testis was removed and frozen on dry ice for transport to PAI. 

The testes were stored frozen at -20°C until processed for counting of 

homogenization-resistant spermatids.

(See Appendix AA for details and methodology.) 

Tissues retained from these evaluations will be discarded following 

issuance of the final report following consultation with the Sponsor. 

2.15. STATISTICAL ANALYSIS

2.15.1. CONTINUOUS DATA

The following parameters were analyzed statistically:

Body weights 

Body weight change 

Feed consumption values 

Organ weight data 

Estrous cycle data

Gestation length 

Pup body weights 

Number of pups (live, dead, total) 

Method of Analysis 

Mean values of all exposure groups were compared to the mean 

value for the control group at each time interval. 
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For all parameters, the standard one-way analysis of variance 

(ANOVA) using the F ratio to assess significance was used 

(Dunlap and Duffy, 1975). If significant differences among the 

means were indicated, additional testing was performed using 

Dunnet’s t-test to determine which means were significantly 

different from the control (Dunlap et al., 1981). All t-tests were 

conducted at the 5% and 1% significance levels. 

Exceptions

Statistical evaluations were not performed when the standard 

deviation for the control group was 0 and/or N (number of animals) 

in the control group was less than or equal to two. 

Dose groups were eliminated from statistical analysis if their 

standard deviation was 0 and/or N (number of animals) in the 

group was less than or equal to two. 

2.15.2. SPERM AND MOTILITY ANALYSIS

The following parameters were analyzed statistically:

Mean sperm count (homogenization-resistant testicular spermatid 

count and caudal epididymal sperm count) and motility data.

A Kruskal-Wallis nonparametric ANOVA test was performed to 

assess significance (Kruskal and Wallis, 1952, 1953). If a 

significant difference occurred (p<0.05), the Wilcoxon (MannWhitney U) test was used for pair-wise comparisons of each 

treated group to the vehicle control group. Statistics were 

performed using an IBM compatible computer with SAS computer 

programs (SAS/STAT User’s Guide, 1989). 

2.15.3. INCIDENCE DATA

The following parameters were analyzed statistically:

Mortality rates

Mating indices (male and female)

Pregnancy rates

Male fertility indices

Live birth indices 
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Pup viability indices (Days 0-4) and lactation indices (Days 4-28) 

Incidence Data Analysis

A Fisher Exact Test with Bonferonni correction was performed to 

identify differences between the groups (Seigel, 1956). 

2.16. DATA STORAGE

All raw data, preserved specimens, and retained samples, as well as the 

original study protocol and the original final report are to be maintained in 

the Archives of the Testing Facility upon completion of the study. The 

Sponsor will determine the final disposition of these materials.

All raw data and all reports generated by Pathology Associates 

International (PAI), Frederick, MD were maintained by PAI. After 

submission of the final report, all of the above will be shipped to 

Huntingdon Life Sciences to be archived. 

2.17. REGULATORY REFERENCES 

2.17.1. TEST GUIDELINE

This study was designed to meet or exceed the pertinent 

requirements of: 

US EPA Vehicle Emissions Inhalation Exposure Guideline 79.61, 

CFR Vol. 59, No. 122, 27 June 1994. 

US EPA (Environmental Protection Agency) Health Effects Test 

Guidelines, OPPTS 870.3800, Reproduction and Fertility Effects 

(August 1998) except this study will only continue through the 1st

generation of offspring.

2.17.2. GOOD LABORATORY PRACTICES

This study was conducted in accordance with US EPA 79.60, CFR 

Vol. 59, No. 122, 27 June 1994 and was performed according to 

the protocol and Huntingdon Life Sciences’ and Pathology 

Associates International’s Standard Operating Procedures. 
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2.17.3. ANIMAL WELFARE ACT COMPLIANCE

This study complied with all appropriate parts of the Animal 

Welfare Act regulations: 9 CFR Parts 1 and 2 Final Rules, Federal 

Register, Volume 54, No. 168, August 31, 1989, pp. 36112-36163 

effective October 30, 1989 and 9 CFR Part 3 Animal Welfare 

Standards; Final Rule, Federal Register, Volume 56, No. 32, 

February 15, 1991, pp. 6426-6505 effective March 18, 1991. 

2.18. PROTOCOL DEVIATIONS

The following protocol deviations occurred during the study but were not 

considered to have compromised the validity or integrity of the study: 

1. Due to supplier error, Charles River Laboratories did not supply male 

and female animals from different rooms. Therefore, the mating of 

male and female littermates, prohibited by protocol, may have 

occurred.

2. Due to technician error, Group II female Animal No. 2543 was not cohoused with a male on 30 July 2002, the mating initiation date. The 

animal was co-housed on 31 July 2002. 

3. Due to technician error, Group II female Animal No. 2542 was found 

pregnant on 30 July 2002 but was not separated from the male until 

31 July 2002. As a result, feed consumption was not initiated until 

31 July 2002 which was Gestation Day 1. 

4. Due to technician error, Group IV female Animal No. 4542 was 

inadvertently loaded into the exposure chamber and exposed on 

Gestation Day 20. 

5. Due to technician error, Group I animals were unloaded from the 

exposure chamber prior to completion of the 30 minute clearing period 

on 25 August 2002. 

6. Due to technician error, the disposition of the extra animals not used 

on study was not recorded in the Study File. The animals would have 

been euthanized or transferred to the in-house colony. 
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7. Due to equipment malfunction, sperm motility images were not 

captured for Group I Animal No. 1026 and Group IV Animal 

No. 4026. 

8. Although not required, a lung floatation test was performed for Pup 

No. 3534-1 which was found dead on Lactation Day 1. 

9. Due to technician error, there is no documentation that the spleen was 

collected for Animal Nos. 1039, 1041, 1043, 1044 and 1049 and no 

saved tissues could be located for these animals.

10. Weanling pups from Dam No. 4538 (pups 2 through 11) were 

euthanized via an intraperitoneal injection of sodium pentobarbital 

instead of by exsanguination after anesthesia with carbon dioxide. 

11. Although partially cannibalized pups were to be discarded, tissues 

were saved for Pup No. 2538-4, found dead and partially cannibalized, 

on Lactation Day 1. 

12. Due to technician error, left testis specimens were stored at -70°C prior 

to processing instead of -20°C. 
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3. RESULTS AND DISCUSSION

3.1. CHAMBER MONITORING

(Appendices A and B)

Chamber distribution analyses (see Table IV in Appendix A) showed that 

the test substance was evenly distributed within each chamber. Chamber 

monitoring (see Table VI in Appendix A) showed that the chamber oxygen 

levels were 20%. Chamber room monitoring (see Table VI in 

Appendix A) showed that no test substance was present in the room and 

that the sound and light levels were acceptable.

The target and mean (± standard deviation) analytical (IR) and nominal 

concentrations are summarized as follows:

Group Test

Substance

Target

Concentration

(mg/m3

) 

Analytical 

Concentration 

(mg/m3

) 

Nominal

Concentration

(mg/m3

) 

I Air Control 0 0 ± 0 0 ± 0

II Gasoline DIPE 

Vapor 

Condensate

2000 2034 ± 128 2086 ± 116

III Gasoline DIPE 

Vapor 

Condensate

10000 10260 ± 621 10231 ± 422 

IV Gasoline DIPE 

Vapor 

Condensate

20000 20230 ± 775 19903 ± 635

The analytically measured exposure levels of the airborne test substance 

were acceptably close, in the opinion of the study director, to the targeted 

exposure levels. The measured and nominal concentrations varied 

somewhat (less than 3%), but acceptably, from the expected 1:1 ratio for 

this type of vapor exposure. Chamber environmental conditions averaged 

25oC temperature and 46% relative humidity. 
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Mean particle size distribution measurements for the exposures are 

summarized as follows:

Group

Test

Substance

Mass Median

Aerodynamic

Diameter

(μm)

Geometric

Standard

Deviation

Total

Mass

Concentration

(mg/m3)

I Air Control 2.478 2.117 6.31 x 10-3

II Gasoline DIPE 

Vapor 

Condensate

1.930 2.074 6.28 x 10-3

III Gasoline DIPE 

Vapor 

Condensate

1.974 2.281 7.75 x 10-3

IV Gasoline DIPE 

Vapor 

Condensate

2.142 2.182 7.83 x 10-3

These results indicated that the atmospheres were essentially vapor only, 

as expected, since there was no substantial difference between the test 

substance chambers and the Air Control chamber. 

Analysis of the major components in the neat test substance and the test 

atmospheres (see Appendix B) showed an acceptably close comparison 

between the neat test substance and the vaporized test substance. This 

data demonstrated that the test animals were exposed, as expected, to all of 

the major components of the test substance in their acceptably proper 

proportion. The data was consistent from week-to-week during the study 

indicating stability of the test substance and the atmosphere generation 

techniques. 
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3.2. PARENTAL DATA (P0 GENERATION)

3.2.1. MORTALITY

(Tables 1, 3 and 4; Appendices C, E and F) 

There was no effect of treatment on survival. One male rat 

(#3043) in the 10000 mg/m3 group was humanely sacrificed on 

Day 23 due to an injury to the animal’s snout. Another male rat 

(#3044) in the 10000 mg/m3 group was found dead on Day 110 

with no prior clinical signs or body weight effects. However, the 

gross necropsy examination found an extremely enlarged spleen 

and liver and discolored thymus. 

One female rat (#2536) in the 2000 mg/m3 group was humanely 

sacrificed on Day 78 (during the gestation phase) due to an injury 

to the animal’s teeth. Another female rat (#4538) in the 

20000 mg/m3 group was humanely sacrificed on Day 106 (during 

the lactation phase) due to an injury to the animal’s snout. 

3.2.2. PHYSICAL OBSERVATION DATA

(Tables 2, 3 and 4; Appendices A, D, E and F)

The test animals were unremarkable in-chamber during the 

exposure periods. 

The test animals were generally unremarkable during the nonexposure periods (afternoon evaluations) during the premating 

period in both sexes, the mating/postmating period in the male rats, 

and the gestation and lactation periods in the female rats. Scattered 

observations such as chromodacryorrhea and lacrimation were 

noted but were not treatment-related.

3.2.3. BODY WEIGHTS – PREMATING PERIOD

(Tables 5 and 6; Appendices G and H)

There were no exposure-related differences in body weights or 

weight changes in the test substance exposed animals compared to 

the Air Control animals. While a few statistically significant 

differences in body weight gain were seen at several intervals in 
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the test substance exposed animals, these were not in a treatmentrelated pattern.

3.2.4. MATERNAL BODY WEIGHTS - GESTATION AND 

LACTATION PERIODS

(Tables 7, 8, 9 and 10; Appendices I, J, K and L)

There were no exposure-related differences in body weights or 

weight changes in the test substance exposed animals compared to 

the Air Control animals. The slightly greater weight gain during 

gestation, well within normal variation, is most likely due to the 

slightly larger litter sizes in the exposed groups. While a 

statistically significant decrease in weight gain was noted for the 

mid-level exposed group from days 21 to 28 during the lactation 

period, this was not in a treatment-related pattern. The variations 

in gain and loss patterns among the groups during lactation suggest 

that these weight changes are natural fluctuations in lactating 

animals and not linked specifically to exposure. 

3.2.5. FEED CONSUMPTION – PREMATING PERIOD

(Table 11; Appendix M)

There were no exposure-related differences in feed consumption in 

the test substance exposed animals compared to the Air Control 

animals. While a few statistically significant differences were 

noted at various intervals, the absolute differences were generally 

small and were not in a treatment-related pattern.

3.2.6. MATERNAL FEED CONSUMPTION - GESTATION AND 

LACTATION PERIODS

(Tables 12 and 13; Appendices N and O)

There were no exposure-related differences in feed consumption 

during the gestation and lactation periods in the test substance 

exposed animals compared to the Air Control animals. While 

statistically significant increases were noted at two intervals for the 

mid-level exposed group during the lactation period, these were not 

considered as adverse responses because modestly increased feed 

consumption (as opposed to decreased feed consumption) 
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especially in the absence of a dose response pattern is not identified

as a potentially toxic effect.

3.2.7. ESTROUS CYCLE DATA – PREMATING PERIOD

(Table 14; Appendix P)

There were no exposure-related differences in estrous cycle data 

(as measured by cycle length and number of estrous cycles) in the 

test substance exposed animals compared to the Air Control 

animals. One female in each of the Control and 10000 mg/m3 

exposed groups showed signs of having entered pseudopregnancy, 

with prolonged diestrus after the first or second estrus while paired 

with a male, and did not produce a litter. One female in each of the 

2000 and 20000 mg/m3 exposed groups also showed this pattern, 

but they were found pregnant and it was concluded that the mating 

evidence at the first estrus while paired with the male had not been 

observed. One female in the 20000 mg/m3 exposed group showed 

an abnormal cycle (irregular length and then persistent estrus) and 

did not become pregnant. These occurrences were considered 

incidental.

3.2.8. MATING, FERTILITY AND GESTATION INDICES

(Tables 15 and 16; Appendices Q and R)

Mating indices for the male rats treated with the test substance 

were comparable to the Air Control group. Mating, fertility and 

gestation indices for the female rats treated with the test substance 

were comparable to the Air Control group and normal for this 

strain of animal. Most of the females in each group mated at the 

first opportunity (first estrus after initial pairing) with only 

occasional animals mating at the second estrus. There were also no 

treatment-related differences in the other reproductive parameters 

up to the time of parturition including the percent of females 

completing delivery and the duration of gestation, when compared 

to the Air Control group. 
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3.2.9. PARTURITION AND LITTER SURVIVAL DATA

(Table 16; Appendices R and S)

There were no treatment related inter-group differences among the 

parturition and pup survival parameters. The number of 

implantation sites per dam, the number of pups delivered (live or 

dead), subsequent viability (to Day 4 and then to Day 21 postpartum) and sex ratio were normal for this strain of animal. 

Statistically significant differences between treated groups and the 

Air Control group were a result of the incidental finding that the 

Air Control group lost more pups due to a total litter loss in one 

dam prior to Day 4 post partum. 

3.3. PUP DATA (F1 GENERATION)

3.3.1. OBSERVATION DATA

(Table 17; Appendix T)

The pups were unremarkable during the lactation period. Scattered 

observations were noted but were not treatment-related.

3.3.2. BODY WEIGHT DATA 

(Tables 16 and 18; Appendix U)

There was a slight, uniform reduction in pup body weight 

performance over the first 4 days after birth across the groups 

exposed to the test substance, as compared with Air Control. This 

was commensurate with the slightly larger live litter size in these 

groups, as compared with Air Control, and was considered not to 

represent an adverse effect of treatment. Following the 

‘standardization’ of live litter size (via culling) at Day 4 after birth, 

pup body weight performance became uniform across all the 

groups. 
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3.4. POSTMORTEM DATA

3.4.1. TERMINAL ESTROUS AND MACROSCOPIC 

POSTMORTEM EVALUATIONS - PARENTAL 

GENERATION

(Table 19; Appendices V and Z)

There were no exposure-related differences in terminal estrous 

evaluations in the test substance exposed animals compared to the 

Air Control animals. Scattered macroscopic postmortem 

observations such as lung discoloration were noted in a treatmentrelated pattern, but in absence of any effects on lung weights or 

histopathology (see following sections), these findings were not 

considered adverse. 

3.4.2. ORGAN WEIGHTS - PARENTAL GENERATION

(Table 21; Appendix X)

Exposure-related effects on organ weights included statistically 

significant increases in kidney weights (absolute and/or relative to 

body and brain weight) at the 2 higher exposure levels in the males. 

These differences for the males were consistent with the 

hydrocarbon nephropathy findings at the 20000 mg/m3 exposure 

level in the 13-week inhalation study 00-6130 with this same test 

substance. However, these same findings were not seen 

microscopically with these test animals. Also, this finding has 

been generally accepted (US EPA 1991. Alpha 2 microglobulin: 

association with chemically induced renal toxicity and neoplasia in 

male rat. In Risk Assessment Forum, p.85. US Govt. Printing 

Office, Washington, DC.) not to be relevant to human risk 

assessment.

Exposure-related effects on organ weights also included 

statistically significant increases in liver weights (absolute and/or 

relative to body weight or brain weight) at the 2 higher exposure 

levels in the males. These may have been the result of enzyme 

induction by the test substance but were not correlated with any 

histopathology finding (see following section). 
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A few other statistically significant differences from Air Control 

were apparent. The differences in testis weight at 20000 mg/m3 

(relative to brain weight only) and epididymis weight at 10000 and 

20000 mg/m3 were of a very small magnitude (in the region of 

5-8%) and were discounted as being of no biological significance. 

Equally, the difference in seminal vesicle/coagulating gland weight 

(approximately 17% higher in the 20000 mg/m3 group) was not 

considered to be of definitive toxicological significance, 

considering also that neither the histopathological examinations 

nor the sperm evaluations revealed any evidence of treatment 

related change in any of these organs (see relevant sections 3.4.3 

and 3.4.6). 

3.4.3. SPERM EVALUATIONS – PARENTAL GENERATION

(Appendix AA)

Mean percent motility, epididymal sperm count, homogenizationresistant testicular spermatid count, sperm morphology and caudal 

epididymis weight were not affected by treatment at an exposure 

level of 20,000 mg/m3. No biologically meaningful differences were 

observed between the study groups.

3.4.4. MACROSCOPIC POSTMORTEM EVALUATIONS - PUPS 

(Table 20; Appendix W) 

There were no exposure-related differences in macroscopic 

postmortem evaluations in the pups feeding from test substance 

exposed animals compared to the pups feeding from Air Control 

animals. Scattered observations such as dilated renal pelvis were 

noted but were not treatment-related.

3.4.5. ORGAN WEIGHTS - PUPS

(Table 22; Appendix Y)

There were no exposure-related differences in organ weights in the 

pups feeding from test substance exposed animals compared to the 

pups feeding from Air Control animals. A few statistically 

significant differences in thymus weights (relative to body weight) 

were noted for the low- and mid-exposure groups. However, these 
Huntingdon Life Sciences 00-4205 Page 41 

211-DIPE-1G Final Report

were not in a treatment-related pattern and were comparable to 

historical control data (see Appendix DD). 

3.4.6. HISTOPATHOLOGICAL EVALUATIONS - PARENTAL 

GENERATION

(Table 23; Appendix Z)

No test substance related microscopic changes were noted in male 

and female reproductive organs or other protocol-specified tissues 

in this study. The remaining microscopic findings were similar to 

those routinely observed in animals of this age and species and 

were considered incidental.

4. CONCLUSION 

Exposure of rats to 2000, 10000 and 20000 mg/m3 of vapor of Gasoline DIPE

Vapor Condensate resulted in no effects on the exposed parental animals prior to 

their termination. However, increases in liver weights in parental male animals 

exposed to 10000 and 20000 mg/m3 of vapor were seen. Increases in kidney 

weights in parental male animals exposed to the two higher exposure levels of 

vapor were consistent with hydrocarbon nephropathy seen in similar animals 

exposed for 13 weeks to this test substance. However, this finding has been 

generally accepted (US EPA 1991. Alpha 2 microglobulin: association with 

chemically induced renal toxicity and neoplasia in male rat. In Risk Assessment 

Forum, p.85. US Govt. Printing Office, Washington, DC.) not to be relevant to 

human risk assessment. Therefore, a no observable effect level (NOEL) of 

2000 mg/m3 was determined. There was no effect at any of the exposure levels on 

reproductive performance in the study, including mating, fertility, parturition, 

lactation and offspring survival and development for 28 days after birth. 

Therefore, with respect to reproductive performance, a no observable effect level 

(NOEL) of 20000 mg/m3 was determined. 
Huntingdon Life Sciences 00-4205 Page 42 

211-DIPE-1G Final Report

REFERENCES

Dunlap, W.P. and Duffy, J.A. 1975. Fortran IV functions for calculating exact 

probabilities associated with z, chi-square, t and f values. Behav. Res. Methods and 

Instrumentations 7: 59-60. 

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV functions for calculating 

probabilities associated with Dunnett’s test. Behav. Res. Methods and Instrumentation

13: 363-366. 

Dunnett, C.W. 1955. A multiple comparison procedure for comparing several treatments 

with a control. Journal of the American Statistical Association 50: 1096-1121. 

Dunnett, C.W. 1964. New tables for multiple comparisons with a control. Biometrics 20-

3: 482-491. 

Games, P.A. and Howell, J.F. 1976. Pairwise multiple comparison procedures with 

unequal n’s and/or variances: a monte-carlo study. Journal of Educational Statistics 1: 

113-125. 

Kruskal, W.H. and Wallis, W.A. 1952. Use of Ranks in One-Criterion Variance Analysis. 

Journal of the American Statistical Association 47: 583-621. 

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Kruskal-Wallis (1952). Journal of the 

American Statistical Association 48: 907-911. 

Mantel, N. 1963. Chi-square test with one degree of freedom: extensions of the MantelHaenszel procedure. Journal of the American Statistical Association 58: 690-700. 

Seigel, S. 1956. Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill.
Huntingdon Life Sciences 00-4205 Page 43

211-DIPE-1G Final Report

Calculations

Female Mating Index:

no. of females with confirmed mating (sperm and/or vaginal plug) plus no. of 

pregnant females without evidence of mating (no sperm or vaginal plug)/no. of 

females placed with males

Female Fertility Index:

no. of females pregnant/no. of females confirmed mating or pregnancy for 

females without evidence of mating 

Male Mating Index:

no. of males with confirmed mating with a female or pregnancy for females 

without evidence of mating/no. of males placed with females

Male Fertility Index:

no. of males mating and impregnating a female plus the no. of males with a 

pregnant female without evidence of mating/no. of males with confirmed mating 

plus no. of males with a pregnant female without evidence of mating

Gestation Index:

no. of females with liveborn/no. of females with confirmed pregnancy

Viability Index:

no. of pups alive Day 4 precull/no. of liveborn pups

Lactation Index:

no. of pups alive Day 21/no. of pups Day 4 postcull

Live Birth Index:

total no. of liveborn pups/total no. of pups born
Huntingdon Life Sciences 00-4205 Page 44

211-DIPE-1G Final Report

General Preface

General Notes

Individual animal data values presented in this report may be rounded. 

Unrounded individual animal data values are used to calculate the reported 

mean and standard deviation values. Therefore, use of the reported individual 

values to reproduce means, standard deviations and/or to perform any 

subsequent calculations may produce minor discrepancies between the 

calculated values and those presented in this report.

Key to Abbreviations

M = Male

F = Female

No = Number

Gen. = General

PG = Pregnant

Group

Group 

Designation

Exposure 

Levels 

(mg/m3)

I Control 0 (air only)

II Low 2,000

III Middle 10,000

IV High 20,000
11-JAN-2010 11:46

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 TABLE 1 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF SURVIVAL AND PREGNANCY 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

No. of males at start N 26 26 26 26 

Premating

 - Died/sacrificed N 0 0 1 0 

Postmating

 - Died/sacrificed N 0 0 1 0 

No. of females at start N 26 26 26 26 

No. of females cohabitated N 26 26 26 26 

 - Without evidence of mating N 1 3 2 4 

 Pregnant N 0 1 0 1 

 Nonpregnant N 1 2 2 3 

Pregnant N 22 24 24 23 

 - Died/sacrificed N 0 1 0 0 

 - Died delivering N 0 0 0 0 

 - Died/sacrificed post partum N 0 0 0 1 

 - Aborted died/sacrificed N 0 0 0 0 

Nonpregnant N 4 2 2 3 

 - Died/sacrificed N 0 0 0 0 

Total females died/sacrificed N 0 1 0 1 

 % 0.0 4.3 0.0 4.5 

Dams delivering N 22 23 24 23 

 - With liveborn pups N 22 23 24 23 

 % 100.0 100.0 100.0 100.0 

 - With all pups stillborn N 0 0 0 0 

 % 0.0 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
Huntingdon Life Sciences 00-4205 Page 46

211-DIPE-1G Final Report

Summary of Clinical Observations

Preface Table 2

Number of animals examined represents the total number of animals observed 

and animals, which were found dead, died accidentally or were killed in a 

moribund condition or at a scheduled sacrifice for a given interval. Deaths 

occurring between scheduled observation intervals are presented at the next 

scheduled interval.

For summarization purposes, descriptive comments [i.e., location of scab(s) 

and/or sore(s), etc.] are not presented in this table. These data are contained in 

the study raw data if needed.

Total represents a cumulative total of all animals with the indicated observation 

one or more times during the study.

Corresponding exposure levels for each group were as follows:

 Group I - 0 mg/m3

 Group II - 2,000 mg/m3

 Group III - 10,000 mg/m3

 Group IV - 20,000 mg/m3

Generation Intervals:

P0 Animals

Premating: Days –7 to 69

Mating: Days 70 to 83

Postmating: Days 84 to 112
11-JAN-2010 11:48

Huntingdon Life Sciences 00-4205 PAGE 47 

 

 TABLE 2 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES SUMMARY OF CLINICAL OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

 GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 III 26 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25

 IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 Normal 

WITHIN NORMAL LIMITS I 26 26 25 25 25 25 24 24 23 22 22 21 21 21 21 20 19 20 26 

 II 26 26 26 26 26 26 26 24 24 24 24 26 24 24 25 25 25 24 26 

 III 26 26 26 26 22 21 22 22 21 20 20 19 19 20 20 21 21 20 26 

 IV 26 26 26 24 24 24 24 23 22 22 21 20 20 20 20 21 21 21 26 

 Dead 

HUMANE SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

FOUND DEAD I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 Gen. Appearance 

SWOLLEN DIGIT I 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

SWOLLEN PAW(S) I 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 
11-JAN-2010 11:48

Huntingdon Life Sciences 00-4205 PAGE 48 

 

 TABLE 2 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES SUMMARY OF CLINICAL OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

 GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 III 26 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25

 IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 Dermal-General 

ULCERATION - CERVICAL I 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

ALOPECIA - I 0 0 0 0 0 0 1 2 2 3 3 3 2 2 2 2 1 1 3 

EXTREMITIES/SNOUT II 0 0 0 0 0 0 0 1 2 2 2 0 1 1 1 1 1 1 3 

 III 0 0 0 0 3 3 3 3 4 4 4 4 4 3 3 3 3 3 5 

 IV 0 0 0 1 1 1 1 2 2 2 3 3 3 2 2 2 1 1 4 

SCABS I 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 

ALOPECIA - GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 1 1 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 Ocular 

CHROMODACRYORRHEA - I 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 

BILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 

 IV 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 
11-JAN-2010 11:48

Huntingdon Life Sciences 00-4205 PAGE 49 

 

 TABLE 2 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES SUMMARY OF CLINICAL OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

 GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

 III 26 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25

 IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

CHROMODACRYORRHEA - I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 3 

UNILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 2 

 IV 0 0 0 0 0 0 0 1 0 1 1 1 1 1 1 1 1 2 2 

LACRIMATION - UNILATERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 

 Oral/Buccal 

INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 0 1 0 1 1 1 1 1 2 2 2 3 

 II 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 1 3 

 III 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 2 

 IV 0 0 0 0 0 0 0 0 1 0 1 2 2 4 3 3 3 3 4 

ORAL SORE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

INCISORS MALOCCLUDED I 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 1 

 IV 0 0 0 0 0 0 0 1 2 1 3 3 3 3 4 3 3 3 4 
11-JAN-2010 11:48

Huntingdon Life Sciences 00-4205 PAGE 50 

 

 TABLE 2 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES SUMMARY OF CLINICAL OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

 GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 22 1 1 1 1 1 1

 II 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 2 2 2

 III 26 26 26 26 26 26 26 26 26 26 26 23 2 2 2 2 2 2

 IV 26 26 26 26 26 26 26 26 26 26 26 23 4 4 4 3 3 3

 Normal 

WITHIN NORMAL LIMITS I 26 26 26 26 26 25 25 23 21 21 21 18 1 1 1 1 1 0 26 

 II 26 26 26 26 26 25 25 25 23 23 21 21 3 3 3 2 2 0 26 

 III 26 26 26 25 26 26 26 25 26 26 25 22 2 2 2 2 2 0 26 

 IV 26 26 26 26 26 25 25 25 24 25 24 21 4 4 4 3 3 0 26 

 Dead 

TERMINAL SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 

 Gen. Appearance 

SWOLLEN DIGIT I 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 

 II 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 Dermal-General 

ALOPECIA - I 0 0 0 0 0 0 1 2 3 3 3 3 0 0 0 0 0 0 3 

EXTREMITIES/SNOUT II 0 0 0 0 0 0 1 1 3 3 4 2 0 0 0 0 0 0 4 

 III 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 2 

 IV 0 0 0 0 0 1 1 1 1 1 1 1 0 0 0 0 0 0 1 
11-JAN-2010 11:48

Huntingdon Life Sciences 00-4205 PAGE 51 

 

 TABLE 2 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES SUMMARY OF CLINICAL OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

 GROUP# -7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 22 1 1 1 1 1 1

 II 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 2 2 2

 III 26 26 26 26 26 26 26 26 26 26 26 23 2 2 2 2 2 2

 IV 26 26 26 26 26 26 26 26 26 26 26 23 4 4 4 3 3 3

ALOPECIA - GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 

 IV 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 

 Ocular 

CHROMODACRYORRHEA - I 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0 0 0 1 

UNILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

LACRIMATION - UNILATERAL I 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 1 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 Oral/Buccal 

INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 1 2 1 1 1 0 0 0 0 0 0 2 

 II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 III 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 

 IV 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 

INCISORS MALOCCLUDED I 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 

 II 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 

 III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

 IV 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 
11-JAN-2010 11:52

Huntingdon Life Sciences 00-4205 PAGE 52 

 

 TABLE 3 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF CLINICAL OBSERVATIONS DURING GESTATION - (frequency/animals) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 to 20

 Normal 

----------

WITHIN NORMAL LIMITS 79/20 69/18 91/23 80/20 

HUMANE SACRIFICE 0/ 0 1/ 1 0/ 0 0/ 0 

 Gen. Appearance 

-------------------

SWOLLEN DIGIT 2/ 1 0/ 0 0/ 0 0/ 0 

MISSING DIGIT 2/ 1 0/ 0 0/ 0 0/ 0 

 Dermal-General 

------------------

ALOPECIA - EXTREMITIES/SNOUT 13/ 4 16/ 5 5/ 2 4/ 1 

ALOPECIA - GENERAL 3/ 1 4/ 1 4/ 1 3/ 1 

 Ocular 

----------

CHROMODACRYORRHEA - BILATERAL 0/ 0 1/ 1 0/ 0 0/ 0 

CHROMODACRYORRHEA - UNILATERAL 4/ 1 0/ 0 0/ 0 0/ 0 

 Oral/Buccal 

---------------

INCISORS BROKEN/MISSING 4/ 1 1/ 1 0/ 0 0/ 0 

INCISORS MALOCCLUDED 0/ 0 0/ 0 0/ 0 4/ 1 

 Room Check 

--------------

DECREASED FECAL VOLUME 0/ 0 1/ 1 0/ 0 0/ 0 
12-JAN-2010 16:13

Huntingdon Life Sciences 00-4205 PAGE 53 

 

 TABLE 4 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MATERNAL CLINICAL OBSERVATIONS DURING LACTATION - (frequency/animals) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 1 to 30

 Normal 

----------

WITHIN NORMAL LIMITS 104/18 107/18 128/23 122/21 

HUMANE SACRIFICE 0/ 0 0/ 0 0/ 0 1/ 1 

TERMINAL SACRIFICE 22/22 23/23 24/24 22/22 

 Gen. Appearance 

-------------------

EMACIATION 1/ 1 0/ 0 0/ 0 0/ 0 

 Dermal-General 

------------------

ALOPECIA - EXTREMITIES/SNOUT 19/ 4 30/ 5 15/ 3 8/ 2 

ALOPECIA - GENERAL 6/ 1 6/ 1 4/ 1 6/ 1 

YELLOW ANO-GENITAL STAINING 1/ 1 0/ 0 0/ 0 0/ 0 

 Ocular 

----------

CHROMODACRYORRHEA - UNILATERAL 6/ 1 0/ 0 0/ 0 0/ 0 

 Oral/Buccal 

---------------

INCISORS BROKEN/MISSING 6/ 1 1/ 1 0/ 0 0/ 0 

INCISORS MALOCCLUDED 0/ 0 0/ 0 0/ 0 6/ 1 

 Room Check 

--------------

INCISORS MALOCCLUDED 0/ 0 0/ 0 0/ 0 1/ 1 

SWOLLEN SNOUT 0/ 0 0/ 0 0/ 0 1/ 1 

RED EXUDATE SNOUT 0/ 0 0/ 0 0/ 0 1/ 1 
11-JAN-2010 11:54

Huntingdon Life Sciences 00-4205 PAGE 54 

 

 TABLE 5 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 MEAN 145 145 145 145 

 S.D. 10.4 10.5 10.5 10.8 

 N 26 26 26 26 

 DAY 0 MEAN 213 212 213 213 

 S.D. 14.1 11.9 13.4 12.8 

 N 26 26 26 26 

 DAY 7 MEAN 267 265 269 266 

 S.D. 17.6 16.9 16.9 17.6 

 N 26 26 26 26 

 DAY 14 MEAN 319 315 321 317 

 S.D. 22.6 21.9 22.1 22.7 

 N 26 26 26 26 

 DAY 21 MEAN 360 357 356 358 

 S.D. 26.6 25.8 27.5 27.1 

 N 26 26 26 26 

 DAY 28 MEAN 389 389 388 387 

 S.D. 29.4 29.6 28.1 30.1 

 N 26 26 25 26 

 DAY 35 MEAN 419 420 418 415 

 S.D. 33.4 34.7 33.2 34.8 

 N 26 26 25 26 

 DAY 42 MEAN 440 446 440 440 

 S.D. 40.8 38.6 35.9 40.1 

 N 26 26 25 26 

 DAY 49 MEAN 462 465 459 459 

 S.D. 37.8 43.1 38.0 41.5 

 N 26 26 25 26 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:54

Huntingdon Life Sciences 00-4205 PAGE 55 

 

 TABLE 5 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 56 MEAN 480 482 480 478 

 S.D. 40.3 45.0 40.4 46.6 

 N 26 26 25 26 

 DAY 63 MEAN 494 499 491 489 

 S.D. 42.2 48.3 41.4 51.3 

 N 26 26 25 26 

 DAY 69 MEAN 506 511 503 496 

 S.D. 45.5 49.5 44.8 58.8 

 N 26 26 25 26 

 DAY 77 MEAN 516 520 513 509 

 S.D. 46.4 50.4 44.7 55.5 

 N 26 26 25 26 

 DAY 84 MEAN 528 535 527 526 

 S.D. 49.7 51.5 45.8 58.1 

 N 26 26 25 26 

 DAY 91 MEAN 535 543 534 536 

 S.D. 52.0 53.6 47.5 57.7 

 N 26 26 25 26 

 DAY 98 MEAN 549 554 545 545 

 S.D. 54.4 55.1 50.8 59.7 

 N 26 26 25 26 

 DAY 105 MEAN 557 562 557 555 

 S.D. 55.9 56.0 49.6 61.8 

 N 26 26 25 26 

 DAY 112 MEAN 570 572 570 566 

 S.D. 57.0 58.0 51.6 63.8 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:54

Huntingdon Life Sciences 00-4205 PAGE 56 

 

 TABLE 5 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 MEAN 109 109 109 109 

 S.D. 7.5 7.9 7.8 7.6 

 N 26 26 26 26 

 DAY 0 MEAN 155 154 150 152 

 S.D. 8.9 10.3 9.3 10.1 

 N 26 26 26 26 

 DAY 7 MEAN 178 179 175 175 

 S.D. 11.2 11.0 11.5 12.5 

 N 26 26 26 26 

 DAY 14 MEAN 204 201 199 198 

 S.D. 13.7 18.2 13.2 16.2 

 N 26 26 26 26 

 DAY 21 MEAN 224 223 220 215 

 S.D. 15.6 14.0 14.8 18.8 

 N 26 26 26 26 

 DAY 28 MEAN 240 238 233 229 

 S.D. 17.0 18.9 16.2 21.1 

 N 26 26 26 26 

 DAY 35 MEAN 248 251 247 242 

 S.D. 21.0 19.8 18.4 21.8 

 N 26 26 26 26 

 DAY 42 MEAN 258 262 259 253 

 S.D. 24.5 20.5 19.1 23.8 

 N 26 26 26 26 

 DAY 49 MEAN 268 268 264 261 

 S.D. 20.3 19.7 19.9 25.3 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:54

Huntingdon Life Sciences 00-4205 PAGE 57 

 

 TABLE 5 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 56 MEAN 273 275 273 267 

 S.D. 21.2 20.4 20.3 25.7 

 N 26 26 26 26 

 DAY 63 MEAN 278 278 276 273 

 S.D. 21.3 23.1 22.5 27.3 

 N 26 26 26 26 

 DAY 69 MEAN 283 283 279 278 

 S.D. 20.6 21.0 20.1 28.7 

 N 26 26 26 26 

 DAY 77 MEAN 264 282 296 280 

 S.D. 0.0 6.6 22.6 20.2 

 N 1 3 2 4 

 DAY 84 MEAN 268 294 301 291 

 S.D. 0.0 14.9 10.0 29.6 

 N 1 3 2 4 

 DAY 91 MEAN 272 303 293 314 

 S.D. 0.0 29.0 19.4 44.5 

 N 1 3 2 4 

 DAY 98 MEAN 284 292 310 285 

 S.D. 18.2 14.5 16.7 29.9 

 N 2 2 3 3 

 DAY 105 MEAN 288 299 311 290 

 S.D. 8.6 7.1 19.8 29.5 

 N 2 2 3 3 

 DAY 112 MEAN 310 307 

 S.D. 0.0 0.0 

 N 1 1 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:54

Huntingdon Life Sciences 00-4205 PAGE 58 

 

 TABLE 5 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT VALUES (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 119 MEAN 311 312 

 S.D. 0.0 0.0 

 N 1 1 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:56

Huntingdon Life Sciences 00-4205 PAGE 59 

 

 TABLE 6 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 TO 0 MEAN 68 67 67 67 

 S.D. 7.8 5.0 7.7 6.2 

 N 26 26 26 26 

 DAY 0 TO 7 MEAN 54 53 56 53 

 S.D. 5.7 6.4 5.6 7.2 

 N 26 26 26 26 

 DAY 7 TO 14 MEAN 52 50 52 52 

 S.D. 7.6 6.3 8.5 8.4 

 N 26 26 26 26 

 DAY 14 TO 21 MEAN 41 42 35 40 

 S.D. 7.2 6.7 22.7 6.6 

 N 26 26 26 26 

 DAY 21 TO 28 MEAN 30 31 29 29 

 S.D. 10.5 6.3 6.8 6.6 

 N 26 26 25 26 

 DAY 28 TO 35 MEAN 29 32 30 28 

 S.D. 6.1 7.0 7.3 7.4 

 N 26 26 25 26 

 DAY 35 TO 42 MEAN 22 26 23 24 

 S.D. 22.2 5.7 5.2 9.3 

 N 26 26 25 26 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:56

Huntingdon Life Sciences 00-4205 PAGE 60 

 

 TABLE 6 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 42 TO 49 MEAN 22 19 19 20 

 S.D. 16.8 6.5 6.8 7.3 

 N 26 26 25 26 

 DAY 49 TO 56 MEAN 18 16 21 19 

 S.D. 7.5 4.3 5.3 8.5 

 N 26 26 25 26 

 DAY 56 TO 63 MEAN 13 17 11 11 

 S.D. 9.5 5.8 5.3 12.3 

 N 26 26 25 26 

 DAY 63 TO 69 MEAN 12 12 12 7 

 S.D. 8.3 5.1 5.4 24.3 

 N 26 26 25 26 

 DAY 69 TO 77 MEAN 10 9 9 13 

 S.D. 9.5 4.9 4.3 15.6 

 N 26 26 25 26 

 DAY 77 TO 84 MEAN 12 15 14 16 

 S.D. 9.9 5.1 5.5 6.1 

 N 26 26 25 26 

 DAY 84 TO 91 MEAN 7 8 8 10 

 S.D. 5.1 6.4 6.2 6.5 

 N 26 26 25 26 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:56

Huntingdon Life Sciences 00-4205 PAGE 61 

 

 TABLE 6 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 91 TO 98 MEAN 14 11 11 9 

 S.D. 4.8 6.3 6.1 6.4 

 N 26 26 25 26 

 DAY 98 TO 105 MEAN 8 8 12* 10 

 S.D. 5.9 5.0 6.5 5.8 

 N 26 26 25 26 

 DAY 105 TO 112 MEAN 13 9* 12 10 

 S.D. 5.7 4.3 5.6 5.6 

 N 26 26 24 26 

 DAY 0 TO 69 MEAN 292 299 291 284 

 S.D. 38.2 41.2 37.6 50.6 

 N 26 26 25 26 

 DAY 0 TO 112 MEAN 356 360 358 353 

 S.D. 48.5 49.7 44.5 54.8 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
11-JAN-2010 11:56

Huntingdon Life Sciences 00-4205 PAGE 62 

 

 TABLE 6 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY -7 TO 0 MEAN 46 45 42 43 

 S.D. 7.1 6.5 6.8 6.5 

 N 26 26 26 26 

 DAY 0 TO 7 MEAN 24 25 25 23 

 S.D. 5.1 5.9 5.8 6.0 

 N 26 26 26 26 

 DAY 7 TO 14 MEAN 26 22 24 24 

 S.D. 5.8 15.2 6.1 6.2 

 N 26 26 26 26 

 DAY 14 TO 21 MEAN 20 22 21 17 

 S.D. 4.4 10.8 5.2 6.2 

 N 26 26 26 26 

 DAY 21 TO 28 MEAN 15 15 12 14 

 S.D. 5.1 8.3 6.7 7.9 

 N 26 26 26 26 

 DAY 28 TO 35 MEAN 8 13 14** 12 

 S.D. 6.8 7.6 6.8 4.8 

 N 26 26 26 26 

 DAY 35 TO 42 MEAN 10 11 13 12 

 S.D. 14.0 5.7 5.2 6.1 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
11-JAN-2010 11:56

Huntingdon Life Sciences 00-4205 PAGE 63 

 

 TABLE 6 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 42 TO 49 MEAN 10 6 5 8 

 S.D. 11.8 5.6 4.3 6.7 

 N 26 26 26 26 

 DAY 49 TO 56 MEAN 5 7 9 6 

 S.D. 5.5 5.6 6.0 6.8 

 N 26 26 26 26 

 DAY 56 TO 63 MEAN 5 4 3 5 

 S.D. 5.8 7.4 5.8 6.3 

 N 26 26 26 26 

 DAY 63 TO 69 MEAN 5 4 3 5 

 S.D. 5.1 6.7 7.6 5.3 

 N 26 26 26 26 

 DAY 0 TO 69 MEAN 129 129 129 126 

 S.D. 16.4 16.6 15.2 22.7 

 N 26 26 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 11:58

Huntingdon Life Sciences 00-4205 PAGE 64 

 

 TABLE 7 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF GESTATION BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 MEAN 280 280 278 275 

 S.D. 19.3 24.9 22.4 28.8 

 N 22 23 24 22 

 DAY 4 MEAN 301 304 301 299 

 S.D. 20.7 23.5 25.0 32.1 

 N 22 22 24 22 

 DAY 7 MEAN 309 313 312 309 

 S.D. 21.6 23.9 26.8 33.7 

 N 22 22 24 22 

 DAY 14 MEAN 336 340 339 337 

 S.D. 24.4 24.7 28.2 35.5 

 N 22 22 24 22 

 DAY 20 MEAN 392 396 393 397 

 S.D. 31.2 30.7 33.6 41.6 

 N 22 22 24 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:00

Huntingdon Life Sciences 00-4205 PAGE 65 

 

 TABLE 8 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF GESTATION BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 0 TO 4 MEAN 22 21 23 23 

 S.D. 5.4 5.3 6.0 7.0 

 N 22 22 24 22 

 DAYS 4 TO 7 MEAN 8 9 11 11 

 S.D. 4.2 3.8 3.2 6.7 

 N 22 22 24 22 

 DAYS 7 TO 14 MEAN 26 27 27 28 

 S.D. 5.4 3.8 4.6 10.2 

 N 22 22 24 22 

 DAYS 14 TO 20 MEAN 56 56 54 60 

 S.D. 14.3 13.2 18.7 11.4 

 N 22 22 24 22 

 DAYS 0 TO 20 MEAN 113 113 115 122 

 S.D. 20.0 15.5 20.4 17.8 

 N 22 22 24 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:00

Huntingdon Life Sciences 00-4205 PAGE 66 

 

 TABLE 9 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 1 MEAN 309 304 306 304 

 S.D. 24.6 22.2 27.8 31.5 

 N 22 23 24 23 

 DAY 4 MEAN 317 323 321 321 

 S.D. 28.5 23.4 27.3 27.5 

 N 21 23 23 23 

 DAY 7 MEAN 318 329 328 329 

 S.D. 35.4 20.9 23.4 28.2 

 N 21 23 23 23 

 DAY 14 MEAN 340 345 346 348 

 S.D. 24.0 19.4 22.6 30.1 

 N 21 23 23 23 

 DAY 21 MEAN 331 334 343 343 

 S.D. 21.8 21.0 23.1 28.3 

 N 21 23 23 22 

 DAY 28 MEAN 308 312 310 311 

 S.D. 24.1 23.3 22.3 29.9 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:01

Huntingdon Life Sciences 00-4205 PAGE 67 

 

 TABLE 10 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 1 TO 4 MEAN 7 19* 16 16 

 S.D. 21.1 8.5 11.2 12.4 

 N 21 23 23 23 

 DAYS 4 TO 7 MEAN 1 6 7 8 

 S.D. 12.5 9.1 6.7 7.5 

 N 21 23 23 23 

 DAYS 7 TO 14 MEAN 22 15 18 19 

 S.D. 22.5 6.6 8.0 8.9 

 N 21 23 23 23 

 DAYS 14 TO 21 MEAN -9 -11 -3 -4 

 S.D. 14.2 13.2 7.5 9.9 

 N 21 23 23 22 

 DAYS 21 TO 28 MEAN -23 -22 -34* -32 

 S.D. 15.8 17.3 11.3 10.5 

 N 21 23 23 22 

 DAYS 1 TO 28 MEAN -1 8 4 8 

 S.D. 12.5 15.8 14.8 17.1 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
11-JAN-2010 12:22

Huntingdon Life Sciences 00-4205 PAGE 68 

 

 TABLE 11 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 MEAN 124 126 128** 130**

 S.D. 4.6 5.0 5.6 6.1 

 N 26 26 25 26 

 DAY 7 MEAN 100 99 100 98 

 S.D. 4.1 4.8 3.4 4.0 

 N 26 26 26 26 

 DAY 14 MEAN 89 86* 87 85**

 S.D. 4.2 3.4 3.2 3.6 

 N 25 26 26 26 

 DAY 21 MEAN 79 77 75 76 

 S.D. 5.3 3.1 8.6 7.3 

 N 26 26 26 26 

 DAY 28 MEAN 70 71 69 70 

 S.D. 4.5 3.2 3.7 4.7 

 N 26 26 25 25 

 DAY 35 MEAN 67 66 67 66 

 S.D. 2.9 2.1 1.9 3.2 

 N 26 26 25 26 

 DAY 42 MEAN 63 63 64 63 

 S.D. 8.0 2.4 2.6 3.4 

 N 26 26 25 26 

 DAY 49 MEAN 63 60 62 61 

 S.D. 5.5 2.6 2.3 3.7 

 N 25 26 24 26 

 DAY 56 MEAN 59 55** 57 57 

 S.D. 4.1 5.1 2.7 3.0 

 N 26 26 25 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 12:22

Huntingdon Life Sciences 00-4205 PAGE 69 

 

 TABLE 11 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 63 MEAN 55 55 54 54 

 S.D. 3.2 2.4 3.2 5.8 

 N 26 26 25 26 

 DAY 69 MEAN 54 53 54 52 

 S.D. 5.0 2.2 2.2 7.8 

 N 26 26 25 26 

 DAY 91 MEAN 50 47** 49 49 

 S.D. 2.4 2.7 1.9 2.6 

 N 26 26 25 26 

 DAY 98 MEAN 49 47* 48 48 

 S.D. 2.5 2.9 2.1 2.7 

 N 26 26 25 26 

 DAY 105 MEAN 48 47 49 49 

 S.D. 3.0 3.2 3.2 2.5 

 N 26 26 25 26 

 DAY 112 MEAN 47 46 48 47 

 S.D. 3.0 2.6 2.2 2.7 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 12:22

Huntingdon Life Sciences 00-4205 PAGE 70 

 

 TABLE 11 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 0 MEAN 127 135** 131 131 

 S.D. 9.8 7.2 6.2 7.6 

 N 26 26 25 26 

 DAY 7 MEAN 104 103 105 101 

 S.D. 8.1 7.1 6.3 6.6 

 N 26 26 22 25 

 DAY 14 MEAN 96 92 94 91* 

 S.D. 6.5 10.1 5.6 6.6 

 N 26 26 25 25 

 DAY 21 MEAN 87 87 86 83* 

 S.D. 4.9 8.1 4.4 4.6 

 N 26 26 26 26 

 DAY 28 MEAN 84 83 83 81 

 S.D. 5.6 5.5 4.0 7.6 

 N 26 26 24 26 

 DAY 35 MEAN 80 78 80 77 

 S.D. 5.1 4.2 4.2 3.3 

 N 25 24 26 26 

 DAY 42 MEAN 76 76 80 75 

 S.D. 9.9 5.5 3.9 4.9 

 N 26 26 26 25 

 DAY 49 MEAN 77 74 76 74 

 S.D. 7.4 5.4 4.6 3.6 

 N 26 25 26 26 

 DAY 56 MEAN 70 71 72 70 

 S.D. 3.8 4.8 5.3 4.9 

 N 26 25 26 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 12:22

Huntingdon Life Sciences 00-4205 PAGE 71 

 

 TABLE 11 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 63 MEAN 66 67 68 70 

 S.D. 4.2 4.6 6.4 9.9 

 N 26 26 26 26 

 DAY 69 MEAN 65 64 66 65 

 S.D. 4.1 5.6 4.4 4.0 

 N 26 26 26 25 

 DAY 91 MEAN 57 64 54 59 

 S.D. 0.0 6.6 0.4 1.7 

 N 1 3 2 4 

 DAY 98 MEAN 54 53 54 56 

 S.D. 0.0 0.4 4.6 3.0 

 N 1 2 2 3 

 DAY 105 MEAN 54 56 54 55 

 S.D. 4.4 2.6 3.2 2.7 

 N 2 2 3 3 

 DAY 112 MEAN 110 130 

 S.D. 0.0 0.0 

 N 1 1 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:23

Huntingdon Life Sciences 00-4205 PAGE 72 

 

 TABLE 12 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF GESTATION FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 0 TO 4 MEAN 72 72 74 72 

 S.D. 5.3 8.2 5.3 5.8 

 N 22 21 24 22 

 DAYS 4 TO 7 MEAN 75 73 76 73 

 S.D. 4.3 7.0 6.2 5.9 

 N 22 22 24 22 

 DAYS 7 TO 14 MEAN 71 71 71 70 

 S.D. 3.9 4.4 3.5 5.5 

 N 22 22 24 22 

 DAYS 14 TO 20 MEAN 64 61 61 62 

 S.D. 3.4 3.8 4.3 3.9 

 N 22 22 23 22 

 DAYS 0 TO 20 MEAN 70 69 71 69 

 S.D. 3.3 4.9 3.7 4.1 

 N 22 22 24 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:25

Huntingdon Life Sciences 00-4205 PAGE 73 

 

 TABLE 13 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF LACTATION FEED CONSUMPTION (GRAMS/KG/DAY) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAYS 1 TO 4 MEAN 90 93 104* 99 

 S.D. 19.6 15.0 15.6 16.8 

 N 21 23 23 22 

 DAYS 4 TO 7 MEAN 108 111 116 113 

 S.D. 14.1 13.4 12.8 11.2 

 N 19 23 23 21 

 DAYS 7 TO 14 MEAN 135 136 143 140 

 S.D. 13.7 15.2 9.4 10.1 

 N 21 23 23 22 

 DAYS 14 TO 21 MEAN 178 183 190 187 

 S.D. 17.3 21.2 17.3 21.6 

 N 21 22 23 21 

 DAYS 21 TO 28 MEAN 324 341 344 337 

 S.D. 60.4 58.2 35.3 41.7 

 N 17 17 22 18 

 DAYS 1 TO 28 MEAN 161 161 178* 168 

 S.D. 20.9 25.8 16.3 22.8 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
11-JAN-2010 12:30

Huntingdon Life Sciences 00-4205 PAGE 74 

 

 TABLE 14 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ESTROUS STAGES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

PRE-COHABITATION PERIOD (21 days evaluated)

 FEMALES EXAMINED N 26 26 26 26 

 CYCLE LENGTH (days) MEAN 4.2 4.2 4.2 4.0 

 S.D. 0.42 0.37 0.32 0.73 

 N 26 26 26 25 

 NUMBER of ESTROUS CYCLES MEAN 4.2 4.0 4.1 4.0 

 S.D. 0.54 0.66 0.59 0.17 

 N 26 26 26 25 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:31

Huntingdon Life Sciences 00-4205 PAGE 75 

 

 TABLE 15 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF COHABITATION DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Females paired with males N 26 26 26 26 

Males placed with females N 26 26 25 26 

 Total number mated N 25 24 23 23 

 male mating index % 96.2 92.3 92.0 88.5 

 with females pregnant N 22 24a 23 23 

 male fertility index % 84.6 92.3 92.0 88.5 

Females with defined

 day 0 of Gestation N 25 23 24 22 

No. of days until Mating MEAN 2.4 3.0 3.2 3.1 

 S.D. 1.12 1.48 1.28 1.70 

 Day 1 to 4 N 24 22 21 18 

 % 96.0 95.7 87.5 81.8 

 Day 5 to 8 N 1 1 3 4 

 % 4.0 4.3 12.5 18.2 

 Day 9 to 14 N 0 0 0 0 

 % 0.0 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences

a

Includes Female No. 2536 which had positive signs of mating and was sacrificed for humane reasons on Gestation Day 1 due to a

snout injury. This animal was presumed pregnant for calculation purposes although this cannot be confirmed.
22-JAN-2010 11:58

Huntingdon Life Sciences 00-4205 PAGE 76 

 

 TABLE 16 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Females on Study N 26 26 26 25 

Females Mated N 25 24 24 22 

 Mating Index % 96.2 92.3 92.3 88.0 

Females Pregnant N 22 24a 24 22 

 Female Fertility Index % 88.0 100.0 100.0 100.0 

Females with Liveborn N 22 23 24 22 

 Gestation Index % 100.0 95.8 100.0 100.0 

Females Completing Delivery N 22 23 24 22 

 % 100.0 95.8 100.0 100.0 

 with Stillborn Pups N 4 2 2 5 

 % 18.2 8.7 8.3 22.7 

 with all Stillborn N 0 0 0 0 

 % 0.0 0.0 0.0 0.0 

 Litters with Liveborn, 

 but no Pups Alive

 day 4 N 1 0 1 0 

 % 4.5 0.0 4.2 0.0 

 day 21 N 1 0 1 0 

 % 4.5 0.0 4.2 0.0 

Duration of Gestation MEAN 21.9 22.0 21.9 22.0 

 S.D. 0.29 0.31 0.41 0.45 

 N 22 22 24 21 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 

a

Includes Female No. 2536 which had positive signs of mating and was sacrificed for humane reasons on Gestation Day 1 due to a

snout injury. This animal was presumed pregnant for calculation purposes although this cannot be confirmed.
22-JAN-2010 11:58

Huntingdon Life Sciences 00-4205 PAGE 77 

 

 TABLE 16 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters with Liveborn Pups N 22 23 24 22 

Pups Delivered (total) N 266 301 314 287 

 MEAN 12.1 13.1 13.1 13.0 

 S.D. 2.81 2.70 2.95 2.08 

 Liveborn N 262 299 312 280 

 Live Birth Index % 98.5 99.3 99.4 97.6 

 Stillborn N 4 2 2 7 

 % 1.5 0.7 0.6 2.4 

 Culled day 4 46 71 78 62 

 Liveborn, not culled N 216 228 234 218 

 prior to day 21

Pups Dying, Missing, and/or Cannibalized

 day 0 N 0 2 0 2 

 % 0.0 0.7 0.0 0.7 

 days 1-4 N 18 3** 4** 2**

 % 6.9 1.0 1.3 0.7 

 days 5-21 N 2 0 2 0 

 % 0.8 0.0 0.6 0.0 

 days 0-4 N 18 5** 4** 4**

 % 6.9 1.7 1.3 1.4 

 days 0-21 N 20 5** 6** 4**

 % 7.6 1.7 1.9 1.4 

Pups Surviving 4 days N 244 294** 308** 276**

 Viability Index % 93.1 98.3 98.7 98.6 

Pups Surviving 21 days N 196 223 228 214 

 Lactation Index % 99.0 100.0 99.1 100.0 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
22-JAN-2010 11:58

Huntingdon Life Sciences 00-4205 PAGE 78 

 

 TABLE 16 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Implantation Sites N 280 325 342 319 

 per Litter MEAN 12.7 14.1 14.3 14.5 

 S.D. 3.21 2.60 2.86 1.71 

Live Pups/Litter

 day 1 MEAN 11.8 12.8 13.4 12.6 

 S.D. 2.76 2.77 1.64 2.34 

 N 22 23 23 22 

 day 4 preculling MEAN 11.1 12.8 13.4* 12.5 

 S.D. 3.70 2.80 1.64 2.44 

 N 22 23 23 22 

 day 4 postculling MEAN 9.0 9.7 10.0 9.7 

 S.D. 2.43 1.26 0.00 0.77 

 N 22 23 23 22 

 day 7 MEAN 9.0 9.7 10.0 9.7 

 S.D. 2.43 1.26 0.00 0.77 

 N 22 23 23 22 

 day 14 MEAN 8.9 9.7 10.0 9.7 

 S.D. 2.45 1.26 0.00 0.77 

 N 22 23 23 22 

 day 21 MEAN 8.9 9.7 9.9 9.7 

 S.D. 2.45 1.26 0.29 0.77 

 N 22 23 23 22 

 day 28 MEAN 8.9 9.7 9.9 9.7 

 S.D. 2.45 1.26 0.29 0.77 

 N 22 23 23 22 

Sex Ratio - Male Pups:Total Pups

 day 0 N 133 152 161 150 

 % 50.8 50.8 51.6 53.8 

 day 21 N 99 112 120 111 

 % 50.5 50.2 52.6 51.9 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 
22-JAN-2010 11:58

Huntingdon Life Sciences 00-4205 PAGE 79 

 

 TABLE 16 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF DELIVERY AND LITTER DATA 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Pup Weight/Litter (grams)

 day 1 MEAN 7.4 7.2 7.0 7.3 

 S.D. 0.81 0.46 0.63 0.49 

 N 22 23 23 22 

 day 4 MEAN 11.4 10.4* 10.2** 10.5* 

 S.D. 1.38 1.18 1.16 0.95 

 N 21 23 23 22 

 day 7 MEAN 14.6 14.6 14.3 14.3 

 S.D. 2.25 1.36 1.51 1.09 

 N 21 23 23 22 

 day 14 MEAN 25.2 25.1 24.5 24.3 

 S.D. 3.39 2.01 2.37 1.82 

 N 21 23 23 22 

 day 21 MEAN 41.9 42.3 40.9 41.2 

 S.D. 5.89 4.20 5.19 3.99 

 N 21 23 23 22 

 day 28 MEAN 78.6 78.8 76.2 76.9 

 S.D. 7.92 6.60 8.22 5.85 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 12:35

Huntingdon Life Sciences 00-4205 PAGE 80 

 

 TABLE 17 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP CLINICAL OBSERVATIONS DURING LACTATION - (frequency/animals) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY 1 to 28

 Normal 

----------

WITHIN NORMAL LIMITS 1029/21 1184/23 1217/23 1166/23 

 Gen. Appearance 

-------------------

SWOLLEN PAW(S) 1/ 1 0/ 0 0/ 0 0/ 0 

DOME HEAD 0/ 0 0/ 0 7/ 1 0/ 0 

SWOLLEN SNOUT 0/ 0 1/ 1 0/ 0 0/ 0 

 Dermal-General 

------------------

ULCERATION - CERVICAL 0/ 0 1/ 1 0/ 0 0/ 0 

SCABS 1/ 1 1/ 1 0/ 0 0/ 0 

 Ocular 

----------

OPACITY - UNILATERAL 0/ 0 2/ 1 0/ 0 0/ 0 
11-JAN-2010 12:36

Huntingdon Life Sciences 00-4205 PAGE 81 

 

 TABLE 18 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 day 1 males MEAN 7.6 7.4 7.2 7.4 

 S.D. 0.81 0.45 0.66 0.55 

 N 22 23 23 22 

 1 females MEAN 7.1 7.0 6.8 7.1 

 S.D. 0.87 0.56 0.62 0.50 

 N 22 23 23 22 

 1 males+females MEAN 7.4 7.2 7.0 7.3 

 S.D. 0.81 0.46 0.63 0.49 

 N 22 23 23 22 

 day 4 males MEAN 11.7 10.6** 10.5** 10.7* 

 preculling S.D. 1.37 1.18 1.18 1.06 

 N 21 23 23 22 

 4 females MEAN 11.0 10.3 9.9* 10.3 

 preculling S.D. 1.46 1.39 1.13 0.92 

 N 21 23 23 22 

 4 males+females MEAN 11.4 10.4* 10.2** 10.5* 

 preculling S.D. 1.38 1.18 1.16 0.95 

 N 21 23 23 22 

 day 4 males MEAN 11.7 10.6** 10.6** 10.7* 

 postculling S.D. 1.37 1.18 1.17 1.12 

 N 21 23 23 22 

 4 females MEAN 11.0 10.3 9.9* 10.3 

 postculling S.D. 1.48 1.39 1.12 0.91 

 N 21 23 23 22 

 4 males+females MEAN 11.4 10.4* 10.3** 10.5* 

 postculling S.D. 1.41 1.19 1.15 0.99 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 12:36

Huntingdon Life Sciences 00-4205 PAGE 82 

 

 TABLE 18 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 day 7 males MEAN 15.0 14.8 14.6 14.7 

 S.D. 2.25 1.43 1.54 1.29 

 N 21 23 23 22 

 7 females MEAN 14.2 14.3 13.9 14.0 

 S.D. 2.30 1.60 1.47 0.97 

 N 21 23 23 22 

 7 males+females MEAN 14.6 14.6 14.3 14.3 

 S.D. 2.25 1.36 1.51 1.09 

 N 21 23 23 22 

 day 14 males MEAN 25.6 25.5 25.0 24.8 

 S.D. 3.39 2.23 2.43 2.03 

 N 21 23 23 22 

 14 females MEAN 24.8 24.7 23.9 23.9 

 S.D. 3.52 2.21 2.35 1.70 

 N 21 23 23 22 

 14 males+females MEAN 25.2 25.1 24.5 24.3 

 S.D. 3.39 2.01 2.37 1.82 

 N 21 23 23 22 

 day 21 males MEAN 42.7 42.8 41.6 42.1 

 S.D. 5.99 4.73 5.54 4.66 

 N 21 23 23 22 

 21 females MEAN 41.2 41.7 40.1 40.5 

 S.D. 6.18 4.30 4.85 3.49 

 N 21 23 23 22 

 21 males+females MEAN 41.9 42.3 40.9 41.2 

 S.D. 5.89 4.20 5.19 3.99 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:36

Huntingdon Life Sciences 00-4205 PAGE 83 

 

 TABLE 18 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS) 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

 day 28 males MEAN 81.8 81.2 79.1 80.0 

 S.D. 8.95 7.83 8.91 7.19 

 N 21 23 23 22 

 28 females MEAN 75.5 76.2 73.0 73.8 

 S.D. 7.72 6.37 7.55 4.96 

 N 21 23 23 22 

 28 males+females MEAN 78.6 78.8 76.2 76.9 

 S.D. 7.92 6.60 8.22 5.85 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:36

Huntingdon Life Sciences 00-4205 PAGE 84 

 

 TABLE 18 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP BODY WEIGHT GAIN -- GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

day 1- 4 males MEAN 4.0 3.2** 3.3* 3.3* 

 S.D. 0.90 0.90 0.67 0.83 

 N 21 23 23 22 

 females MEAN 3.8 3.2 3.1* 3.2* 

 S.D. 0.90 0.97 0.66 0.56 

 N 21 23 23 22 

 males+females MEAN 3.9 3.2* 3.2* 3.2* 

 S.D. 0.88 0.87 0.66 0.62 

 N 21 23 23 22 

day 4- 7 males MEAN 3.3 4.2* 4.1* 3.9 

 S.D. 1.81 0.73 0.69 0.67 

 N 21 23 23 22 

 females MEAN 3.1 4.0* 4.0* 3.8 

 S.D. 1.78 0.67 0.62 0.61 

 N 21 23 23 22 

 males+females MEAN 3.2 4.1** 4.0* 3.9 

 S.D. 1.80 0.66 0.65 0.63 

 N 21 23 23 22 

day 4-21 males MEAN 31.0 32.2 31.1 31.4 

 S.D. 5.44 4.21 4.71 4.03 

 N 21 23 23 22 

 females MEAN 30.2 31.4 30.1 30.2 

 S.D. 5.57 3.54 4.02 3.12 

 N 21 23 23 22 

 males+females MEAN 30.5 31.9 30.7 30.8 

 S.D. 5.34 3.67 4.35 3.52 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 12:36

Huntingdon Life Sciences 00-4205 PAGE 85 

 

 TABLE 18 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP BODY WEIGHT GAIN -- GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

day 4-28 males MEAN 70.1 70.6 68.5 69.2 

 S.D. 8.29 7.30 8.13 6.51 

 N 21 23 23 22 

 females MEAN 64.5 65.9 63.1 63.6 

 S.D. 6.99 5.70 6.82 4.56 

 N 21 23 23 22 

 males+females MEAN 67.2 68.4 66.0 66.4 

 S.D. 7.23 6.08 7.45 5.35 

 N 21 23 23 22 

day 7-14 males MEAN 10.6 10.6 10.4 10.1 

 S.D. 1.46 1.29 1.29 1.22 

 N 21 23 23 22 

 females MEAN 10.7 10.4 10.0 9.9 

 S.D. 1.70 1.13 1.34 1.17 

 N 21 23 23 22 

 males+females MEAN 10.6 10.5 10.2 10.0 

 S.D. 1.49 1.15 1.28 1.19 

 N 21 23 23 22 

day 7-28 males MEAN 66.8 66.4 64.5 65.3 

 S.D. 7.02 6.79 7.68 6.19 

 N 21 23 23 22 

 females MEAN 61.4 61.9 59.1 59.8 

 S.D. 5.98 5.35 6.40 4.24 

 N 21 23 23 22 

 males+females MEAN 64.0 64.3 62.0 62.6 

 S.D. 6.01 5.66 7.01 5.03 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:36

Huntingdon Life Sciences 00-4205 PAGE 86 

 

 TABLE 18 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP BODY WEIGHT GAIN -- GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

day 14-21 males MEAN 17.1 17.3 16.6 17.3 

 S.D. 3.12 2.78 3.32 2.99 

 N 21 23 23 22 

 females MEAN 16.4 17.0 16.1 16.6 

 S.D. 3.18 2.46 2.90 2.17 

 N 21 23 23 22 

 males+females MEAN 16.7 17.2 16.4 16.9 

 S.D. 3.02 2.50 3.10 2.53 

 N 21 23 23 22 

day 14-28 males MEAN 56.3 55.7 54.1 55.2 

 S.D. 5.79 5.86 6.68 5.46 

 N 21 23 23 22 

 females MEAN 50.7 51.5 49.1 49.9 

 S.D. 4.52 4.65 5.60 3.55 

 N 21 23 23 22 

 males+females MEAN 53.4 53.7 51.8 52.6 

 S.D. 4.76 4.93 6.12 4.31 

 N 21 23 23 22 

day 21-28 males MEAN 39.2 38.4 37.5 37.9 

 S.D. 3.71 3.60 3.86 2.94 

 N 21 23 23 22 

 females MEAN 34.3 34.5 32.9 33.3 

 S.D. 2.25 2.82 3.21 2.17 

 N 21 23 23 22 

 males+females MEAN 36.7 36.6 35.3 35.7 

 S.D. 2.74 3.00 3.50 2.34 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:38

Huntingdon Life Sciences 00-4205 PAGE 87 

 

 TABLE 19 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26 

GROSS EXAM N 0 0 1 0 

----------

FRACTURE(S) N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

THYMUS N 1 3 2 1 

------

DISCOLORED FOCUS/FOCI N 1 3 2 1 

 % 3.8 11.5 7.7 3.8 

LUNGS N 1 3 5 6 

-----

 DISCOLORED FOCI N 1 3 5 6 

 % 3.8 11.5 19.2 23.1 

SPLEEN N 0 0 1 0 

------

ENLARGED N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:38

Huntingdon Life Sciences 00-4205 PAGE 88 

 

 TABLE 19 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26 

LIVER N 2 0 2 0 

-----

ENLARGED N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

DISCOLORED FOCI N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

DISCOLORED AREA(S) N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

ADHESION N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

NODULE(S) N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

KIDNEY N 3 3 4 3 

------

 DILATED RENAL PELVIS N 3 3 3 2 

 % 11.5 11.5 11.5 7.7 

DISCOLORED FOCUS/FOCI N 0 0 0 1 

 % 0.0 0.0 0.0 3.8 

 CYSTIC KIDNEY N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:38

Huntingdon Life Sciences 00-4205 PAGE 89 

 

 TABLE 19 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26 

GONADS N 0 1 0 1 

------

DISCOLORED FOCI ON PROSTATE N 0 1 0 1 

 % 0.0 3.8 0.0 3.8 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:38

Huntingdon Life Sciences 00-4205 PAGE 90 

 

 TABLE 19 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALES N 26 26 26 26 

GROSS EXAM N 0 1 0 1 

----------

FRACTURE(S) N 0 1 0 0 

 % 0.0 3.8 0.0 0.0 

HEMORRHAGE N 0 0 0 1 

 % 0.0 0.0 0.0 3.8 

EYES N 0 1 0 0 

----

OPACITY N 0 1 0 0 

 % 0.0 3.8 0.0 0.0 

THYMUS N 0 0 1 0 

------

DISCOLORED FOCUS/FOCI N 0 0 1 0 

 % 0.0 0.0 3.8 0.0 

THORACIC CAVITY N 1 0 0 0 

---------------

ENLARGED LYMPH NODE(S) N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 12:38

Huntingdon Life Sciences 00-4205 PAGE 91 

 

 TABLE 19 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PARENTAL NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

----------------------------------------------------------------------------------------------------------------------------------- 

FEMALES N 26 26 26 26 

LUNGS N 4 7 11 10 

-----

 DISCOLORED FOCI N 4 7 11 10 

 % 15.4 26.9 42.3 38.5 

SPLEEN N 0 0 0 1 

------

ADHESION N 0 0 0 1 

 % 0.0 0.0 0.0 3.8 

ADRENALS N 1 0 0 0 

--------

 DISCOLORED ADRENALS N 1 0 0 0 

 % 3.8 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 92 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

GROSS EXAM 

----------

 Litter Incidence N 4 1 3 0 

 Pup Incidence N 15 1 3 0 

CANNIBALIZED 

 Pup Incidence N 1 1 1 0 

 % 0.5 0.5 0.5 0.0 

 Litter Incidence N 1 1 1 0 

 % 4.5 4.3 4.2 0.0 

SEX UNDETERMINED 

 Pup Incidence N 0 0 1 0 

 % 0.0 0.0 0.5 0.0 

 Litter Incidence N 0 0 1 0 

 % 0.0 0.0 4.2 0.0 

AUTOLYSIS 

 Pup Incidence N 14 0 3 0 

 % 7.4 0.0 1.4 0.0 

 Litter Incidence N 4 0 3 0 

 % 18.2 0.0 12.5 0.0 

EYES 

----

 Litter Incidence N 0 0 2 0 

 Pup Incidence N 0 0 2 0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 93 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

----------------------------------------------------------------------------------------------------------------------------------- 

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

OPACITY 

 Pup Incidence N 0 0 2 0 

 % 0.0 0.0 0.9 0.0 

 Litter Incidence N 0 0 2 0 

 % 0.0 0.0 8.3 0.0 

THYMUS 

------

 Litter Incidence N 1 0 0 1 

 Pup Incidence N 1 0 0 1 

DISCOLORED FOCI 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 0.5 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 4.5 

SMALL 

 Pup Incidence N 1 0 0 0 

 % 0.5 0.0 0.0 0.0 

 Litter Incidence N 1 0 0 0 

 % 4.5 0.0 0.0 0.0 

LUNGS 

-----

 Litter Incidence N 3 4 2 5 

 Pup Incidence N 3 4 2 8 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 94 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

LUNG FLOTATION TEST-STILLBORN 

 Pup Incidence N 3 2 1 7 

 % 1.6 0.9 0.5 3.5 

 Litter Incidence N 3 2 1 5 

 % 13.6 8.7 4.2 22.7 

LUNG FLOTATION TEST - FOUND DEAD 

 Pup Incidence N 0 2 1 1 

 % 0.0 0.9 0.5 0.5 

 Litter Incidence N 0 2 1 1 

 % 0.0 8.7 4.2 4.5 

STOMACH 

-------

 Litter Incidence N 3 4 2 5 

 Pup Incidence N 6 4 2 8 

 NO MILK IN STOMACH 

 Pup Incidence N 5 3 2 8 

 % 2.6 1.4 0.9 4.0 

 Litter Incidence N 3 3 2 5 

 % 13.6 13.0 8.3 22.7 

SMALL 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 0.5 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 4.5 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 95 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

MILK IN STOMACH 

 Pup Incidence N 1 1 0 0 

 % 0.5 0.5 0.0 0.0 

 Litter Incidence N 1 1 0 0 

 % 4.5 4.3 0.0 0.0 

KIDNEY 

------

 Litter Incidence N 4 3 3 3 

 Pup Incidence N 4 4 4 4 

M FUSED KIDNEY 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 0.5 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 4.5 

ENLARGED KIDNEY(S) 

 Pup Incidence N 0 0 1 0 

 % 0.0 0.0 0.5 0.0 

 Litter Incidence N 0 0 1 0 

 % 0.0 0.0 4.2 0.0 

CYST(S) 

 Pup Incidence N 1 1 3 0 

 % 0.5 0.5 1.4 0.0 

 Litter Incidence N 1 1 2 0 

 % 4.5 4.3 8.3 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 96 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

DILATED RENAL PELVIS 

 Pup Incidence N 3 3 1 3 

 % 1.6 1.4 0.5 1.5 

 Litter Incidence N 3 2 1 2 

 % 13.6 8.7 4.2 9.1 

DISCOLORED 

 Pup Incidence N 0 0 1 0 

 % 0.0 0.0 0.5 0.0 

 Litter Incidence N 0 0 1 0 

 % 0.0 0.0 4.2 0.0 

INTESTINES 

----------

 Litter Incidence N 0 0 0 1 

 Pup Incidence N 0 0 0 1 

A UNDERDEVELOPMENT OF INTESTINE 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 0.5 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 4.5 

ANAL ATRESIA 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 0.5 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 4.5 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 97 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

GONADS 

------

 Litter Incidence N 5 5 4 6 

 Pup Incidence N 8 8 4 6 

UNDESCENDED TESTE(S) 

 Pup Incidence N 8 8 4 6 

 % 4.2 3.8 1.8 3.0 

 Litter Incidence N 5 5 4 6 

 % 22.7 21.7 16.7 27.3 

ABDOMINAL CAVITY

----------------

 Litter Incidence N 1 0 0 1 

 Pup Incidence N 1 0 0 1 

GASTROSCHISIS 

 Pup Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 0.5 

 Litter Incidence N 0 0 0 1 

 % 0.0 0.0 0.0 4.5 

DIAPHRAGMATIC HERNIA 

 Pup Incidence N 1 0 0 0 

 % 0.5 0.0 0.0 0.0 

 Litter Incidence N 1 0 0 0 

 % 4.5 0.0 0.0 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
22-JAN-2010 12:34

Huntingdon Life Sciences 00-4205 PAGE 98 

 

 TABLE 20 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP NECROPSY OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22 

Pups Evaluated N 190 211 221 201 

 Live N 186 209 219 194 

 Stillborn N 4 2 2 7 

HEAD 

----

 Litter Incidence N 0 0 1 0 

 Pup Incidence N 0 0 1 0 

V DOMED HEAD 

 Pup Incidence N 0 0 1 0 

 % 0.0 0.0 0.5 0.0 

 Litter Incidence N 0 0 1 0 

 % 0.0 0.0 4.2 0.0 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
Huntingdon Life Sciences 00-4205 Page 99

211-DIPE-1G Final Report

Mean Parental Organ Weights

Preface Table 21

Table of Contents

P0 Generation Absolute Organ Weights........................................................... 100

P0 Generation Organ to Body Weight Ratios ................................................... 104

P0 Generation Organ to Brain Weight Ratios ................................................... 108

Key to Abbreviations:

g = Grams

Sem.Ves & Co.GL. = Seminal Vesicles and Coagulating Glands
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 100 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ABSOLUTE ORGAN WEIGHTS 

MALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FINAL BODY WEIGHT g MEAN 575 576 575 568 

 S.D. 58.0 59.5 53.0 64.9 

 N 26 26 24 26 

TESTIS - RIGHT g MEAN 1.7060 1.6767 1.7498 1.7651 

 S.D. .14280 .09950 .13300 .12232 

 N 26 26 24 26 

TESTIS - LEFT g MEAN 1.7065 1.6774 1.7594 1.7527 

 S.D. .15133 .08929 .14124 .12487 

 N 26 26 24 26 

TESTES g MEAN 3.4053 3.3432 3.4960 3.5072 

 S.D. .29263 .17706 .26960 .24313 

 N 26 26 24 26 

PROSTATE g MEAN 1.2088 1.1442 1.2587 1.1935 

 S.D. .25577 .19400 .25284 .25560 

 N 26 26 24 26 

SEM.VES & CO.GL. g MEAN 1.8887 1.8686 1.9957 2.1836**

 S.D. .34371 .36861 .32072 .33852 

 N 26 26 24 26 

EPIDIDYMIS RIGHT g MEAN 0.7065 0.7342 0.7542* 0.7485* 

 S.D. .06035 .05919 .05040 .06290 

 N 26 25 24 26 

EPIDIDYMIS LEFT g MEAN 0.7025 0.7200 0.7507* 0.7339 

 S.D. .06496 .05654 .05279 .06036 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 101 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ABSOLUTE ORGAN WEIGHTS 

MALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

----------------------------------------------------------------------------------------------------------------------------------- 

EPIDIDYMIDES g MEAN 1.3979 1.4429 1.4969** 1.4742* 

 S.D. .11588 .09958 .09790 .10475 

 N 26 26 24 26 

ADRENALS g MEAN 0.0632 0.0646 0.0680 0.0607 

 S.D. .00800 .01165 .01189 .01061 

 N 26 26 24 26 

BRAIN g MEAN 2.1742 2.1648 2.1704 2.1301 

 S.D. .10816 .06601 .08024 .08252 

 N 26 26 24 26 

KIDNEYS g MEAN 4.1123 4.0631 4.6034** 4.7256**

 S.D. .51983 .38374 .47749 .53666 

 N 26 26 24 26 

LIVER g MEAN 19.337 19.551 20.315 21.225* 

 S.D. 2.3574 2.3028 2.6331 2.5354 

 N 26 26 24 26 

LUNGS g MEAN 1.8452 1.8113 1.8679 1.8236 

 S.D. .26034 .18217 .21915 .15627 

 N 26 26 24 26 

PITUITARY g MEAN 0.0120 0.0114 0.0120 0.0118 

 S.D. .00147 .00207 .00133 .00207 

 N 26 26 24 26 

SPLEEN g MEAN 0.8145 0.7693 0.8396 0.7907 

 S.D. .13658 .10398 .12317 .10640 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 102 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ABSOLUTE ORGAN WEIGHTS 

FEMALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FINAL BODY WEIGHT g MEAN 308 312 310 311 

 S.D. 24.1 23.3 22.3 29.9 

 N 21 23 23 22 

ADRENALS g MEAN 0.0734 0.0751 0.0728 0.0767 

 S.D. .00770 .01102 .01314 .01069 

 N 21 23 23 22 

BRAIN g MEAN 1.9398 1.9219 1.9267 1.9395 

 S.D. .07778 .08865 .06155 .07416 

 N 21 23 23 22 

KIDNEYS g MEAN 2.5653 2.5942 2.6382 2.6944 

 S.D. .18529 .18497 .19552 .25326 

 N 21 23 23 22 

LIVER g MEAN 13.920 13.849 14.350 14.506 

 S.D. 1.6664 1.7315 1.5820 2.1402 

 N 21 23 23 22 

LUNGS g MEAN 1.4551 1.4585 1.4780 1.4882 

 S.D. .10850 .12881 .11823 .16740 

 N 21 23 23 22 

PITUITARY g MEAN 0.0143 0.0159 0.0145 0.0156 

 S.D. .00271 .00341 .00207 .00345 

 N 21 23 23 22 

SPLEEN g MEAN 0.5869 0.5995 0.6025 0.6148 

 S.D. .07949 .06532 .07758 .09543 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 103 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ABSOLUTE ORGAN WEIGHTS 

FEMALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

OVARY RIGHT g MEAN 0.0577 0.0550 0.0565 0.0585 

 S.D. .01177 .01297 .00880 .01266 

 N 21 23 23 22 

OVARY LEFT g MEAN 0.0529 0.0554 0.0532 0.0583 

 S.D. .01332 .01074 .00974 .01005 

 N 21 23 23 22 

OVARIES g MEAN 0.1100 0.1100 0.1097 0.1161 

 S.D. .02113 .01881 .01548 .01902 

 N 21 23 23 22 

UTERUS/OVIDUCTS g MEAN 0.7000 0.7036 0.6054 0.6729 

 S.D. .19015 .19208 .14815 .19628 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 104 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO 

MALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FINAL BODY WEIGHT g MEAN 575 576 575 568 

 S.D. 58.0 59.5 53.0 64.9 

 N 26 26 24 26 

TESTIS - RIGHT Ratio MEAN 0.0030 0.0029 0.0031 0.0031 

 S.D. .00033 .00038 .00033 .00035 

 N 26 26 24 26 

TESTIS - LEFT Ratio MEAN 0.0030 0.0029 0.0031 0.0031 

 S.D. .00033 .00037 .00035 .00034 

 N 26 26 24 26 

TESTES Ratio MEAN 0.0060 0.0059 0.0061 0.0062 

 S.D. .00066 .00073 .00068 .00069 

 N 26 26 24 26 

PROSTATE Ratio MEAN 0.0021 0.0020 0.0022 0.0021 

 S.D. .00046 .00041 .00044 .00052 

 N 26 26 24 26 

SEM.VES & CO.GL. Ratio MEAN 0.0033 0.0033 0.0035 0.0039**

 S.D. .00061 .00065 .00064 .00076 

 N 26 26 24 26 

EPIDIDYMIS RIGHT Ratio MEAN 0.0012 0.0013 0.0013 0.0013 

 S.D. .00015 .00017 .00014 .00015 

 N 26 25 24 26 

EPIDIDYMIS LEFT Ratio MEAN 0.0012 0.0013 0.0013 0.0013 

 S.D. .00014 .00014 .00016 .00014 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 105 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO 

MALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

EPIDIDYMIDES Ratio MEAN 0.0024 0.0025 0.0026 0.0026 

 S.D. .00028 .00029 .00029 .00028 

 N 26 26 24 26 

ADRENALS Ratio MEAN 0.0001 0.0001 0.0001 0.0001 

 S.D. .00002 .00002 .00002 .00002 

 N 26 26 24 26 

BRAIN Ratio MEAN 0.0038 0.0038 0.0038 0.0038 

 S.D. .00034 .00039 .00032 .00035 

 N 26 26 24 26 

KIDNEYS Ratio MEAN 0.0071 0.0071 0.0080** 0.0084**

 S.D. .00048 .00053 .00048 .00089 

 N 26 26 24 26 

LIVER Ratio MEAN 0.0336 0.0339 0.0352** 0.0374**

 S.D. .00159 .00147 .00255 .00188 

 N 26 26 24 26 

LUNGS Ratio MEAN 0.0032 0.0032 0.0033 0.0032 

 S.D. .00039 .00026 .00031 .00032 

 N 26 26 24 26 

PITUITARY Ratio MEAN 0.0000 0.0000 0.0000 0.0000 

 S.D. .00000 .00000 .00000 .00000 

 N 26 26 24 26 

SPLEEN Ratio MEAN 0.0014 0.0013 0.0015 0.0014 

 S.D. .00019 .00023 .00018 .00016 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 106 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO 

FEMALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

FINAL BODY WEIGHT g MEAN 308 312 310 311 

 S.D. 24.1 23.3 22.3 29.9 

 N 21 23 23 22 

ADRENALS Ratio MEAN 0.0002 0.0002 0.0002 0.0002 

 S.D. .00002 .00004 .00005 .00003 

 N 21 23 23 22 

BRAIN Ratio MEAN 0.0063 0.0062 0.0062 0.0063 

 S.D. .00053 .00045 .00040 .00050 

 N 21 23 23 22 

KIDNEYS Ratio MEAN 0.0083 0.0083 0.0085 0.0087 

 S.D. .00044 .00050 .00055 .00050 

 N 21 23 23 22 

LIVER Ratio MEAN 0.0451 0.0443 0.0463 0.0465 

 S.D. .00281 .00410 .00319 .00346 

 N 21 23 23 22 

LUNGS Ratio MEAN 0.0047 0.0047 0.0048 0.0048 

 S.D. .00033 .00042 .00028 .00034 

 N 21 23 23 22 

PITUITARY Ratio MEAN 0.0000 0.0001 0.0000 0.0000 

 S.D. .00001 .00001 .00001 .00001 

 N 21 23 23 22 

SPLEEN Ratio MEAN 0.0019 0.0019 0.0019 0.0020 

 S.D. .00024 .00017 .00021 .00027 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 107 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO 

FEMALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

OVARY RIGHT Ratio MEAN 0.0002 0.0002 0.0002 0.0002 

 S.D. .00004 .00004 .00003 .00004 

 N 21 23 23 22 

OVARY LEFT Ratio MEAN 0.0002 0.0002 0.0002 0.0002 

 S.D. .00004 .00004 .00003 .00003 

 N 21 23 23 22 

OVARIES Ratio MEAN 0.0004 0.0004 0.0004 0.0004 

 S.D. .00007 .00006 .00005 .00006 

 N 21 23 23 22 

UTERUS/OVIDUCTS Ratio MEAN 0.0023 0.0023 0.0020 0.0022 

 S.D. .00062 .00063 .00054 .00058 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 108 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO 

MALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

TESTIS - RIGHT Ratio MEAN 0.7850 0.7751 0.8068 0.8298* 

 S.D. .05835 .04972 .06127 .06579 

 N 26 26 24 26 

TESTIS - LEFT Ratio MEAN 0.7854 0.7752 0.8112 0.8238 

 S.D. .06432 .04074 .06525 .06481 

 N 26 26 24 26 

TESTES Ratio MEAN 1.5670 1.5453 1.6120 1.6487* 

 S.D. .12206 .08643 .12535 .12908 

 N 26 26 24 26 

PROSTATE Ratio MEAN 0.5568 0.5291 0.5802 0.5618 

 S.D. .11538 .09237 .11568 .12730 

 N 26 26 24 26 

SEM.VES & CO.GL. Ratio MEAN 0.8710 0.8640 0.9214 1.0247**

 S.D. .16401 .17203 .15383 .14796 

 N 26 26 24 26 

EPIDIDYMIS RIGHT Ratio MEAN 0.3252 0.3397 0.3478* 0.3518**

 S.D. .02661 .02877 .02472 .03174 

 N 26 25 24 26 

EPIDIDYMIS LEFT Ratio MEAN 0.3231 0.3329 0.3462* 0.3449* 

 S.D. .02522 .02785 .02609 .03031 

 N 26 26 24 26 

EPIDIDYMIDES Ratio MEAN 0.6433 0.6671 0.6904** 0.6928**

 S.D. .04846 .04972 .04879 .05363 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 109 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO 

MALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS Ratio MEAN 0.0292 0.0298 0.0314 0.0285 

 S.D. .00428 .00540 .00544 .00471 

 N 26 26 24 26 

KIDNEYS Ratio MEAN 1.8890 1.8772 2.1207** 2.2196**

 S.D. .19685 .16948 .20311 .24610 

 N 26 26 24 26 

LIVER Ratio MEAN 8.8903 9.0315 9.3552 9.9636**

 S.D. .96476 1.0201 1.1247 1.1240 

 N 26 26 24 26 

LUNGS Ratio MEAN 0.8482 0.8365 0.8599 0.8563 

 S.D. .10786 .07613 .08880 .06876 

 N 26 26 24 26 

PITUITARY Ratio MEAN 0.0055 0.0053 0.0055 0.0055 

 S.D. .00062 .00097 .00056 .00092 

 N 26 26 24 26 

SPLEEN Ratio MEAN 0.3752 0.3556 0.3871 0.3712 

 S.D. .06294 .04879 .05626 .04767 

 N 26 26 24 26 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.01 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 110 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO 

FEMALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS Ratio MEAN 0.0379 0.0391 0.0378 0.0395 

 S.D. .00462 .00560 .00665 .00523 

 N 21 23 23 22 

KIDNEYS Ratio MEAN 1.3246 1.3515 1.3695 1.3890 

 S.D. .11119 .09915 .09656 .11608 

 N 21 23 23 22 

LIVER Ratio MEAN 7.1818 7.2160 7.4444 7.4674 

 S.D. .84545 .90048 .75483 .95689 

 N 21 23 23 22 

LUNGS Ratio MEAN 0.7505 0.7588 0.7669 0.7670 

 S.D. .05244 .05567 .05428 .07663 

 N 21 23 23 22 

PITUITARY Ratio MEAN 0.0074 0.0083 0.0075 0.0080 

 S.D. .00147 .00176 .00102 .00159 

 N 21 23 23 22 

SPLEEN Ratio MEAN 0.3033 0.3123 0.3128 0.3170 

 S.D. .04462 .03379 .03959 .04714 

 N 21 23 23 22 

OVARY RIGHT Ratio MEAN 0.0298 0.0286 0.0293 0.0301 

 S.D. .00632 .00632 .00452 .00654 

 N 21 23 23 22 

OVARY LEFT Ratio MEAN 0.0273 0.0289 0.0276 0.0301 

 S.D. .00710 .00566 .00486 .00503 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 13:21

Huntingdon Life Sciences 00-4205 PAGE 111 

 

 TABLE 21 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO 

FEMALES 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

OVARIES Ratio MEAN 0.0568 0.0572 0.0569 0.0599 

 S.D. .01149 .00918 .00766 .00970 

 N 21 23 23 22 

UTERUS/OVIDUCTS Ratio MEAN 0.3629 0.3680 0.3140 0.3471 

 S.D. .10607 .10587 .07336 .10174 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
Huntingdon Life Sciences 00-4205 Page 112

211-DIPE-1G Final Report

Mean Pup Organ Weights

Preface Table 22

Table of Contents

F1 Generation Absolute Organ Weights ........................................................... 113

F1 Generation % Organ to Body Weight Ratios................................................ 114

F1 Generation % Organ to Brain Weight Ratios................................................ 115

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m3

Group II - 2,000 mg/m3

Group III - 10,000 mg/m3

Group IV - 20,000 mg/m3
11-JAN-2010 13:28

Huntingdon Life Sciences 00-4205 PAGE 113 

 

 TABLE 22 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP ORGAN WEIGHT DATA 

 PUP ORGAN WEIGHTS IN GRAMS 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

BRAIN MEAN 1.5557 1.5503 1.5423 1.5353 

 S.D. .05827 .05893 .07235 .04089 

 N 21 23 23 22 

 of Male Pups MEAN 1.5853 1.5684 1.5673 1.5608 

 S.D. .07752 .09306 .08504 .06183 

 N 21 23 23 22 

 of Female Pups MEAN 1.5262 1.5322 1.5172 1.5100 

 S.D. .05328 .05710 .07169 .03494 

 N 21 23 23 22 

SPLEEN MEAN 0.3267 0.3108 0.3169 0.2993 

 S.D. .05122 .04533 .05512 .04018 

 N 21 23 23 22 

 of Male Pups MEAN 0.3464 0.3232 0.3418 0.3115 

 S.D. .06832 .06291 .07812 .05702 

 N 21 23 23 22 

 of Female Pups MEAN 0.3070 0.2984 0.2920 0.2871 

 S.D. .05564 .03633 .04735 .04544 

 N 21 23 23 22 

THYMUS MEAN 0.3384 0.3726 0.3701 0.3511 

 S.D. .06328 .06684 .06418 .05561 

 N 21 23 23 22 

 of Male Pups MEAN 0.3378 0.3700 0.3671 0.3440 

 S.D. .08071 .07689 .09186 .08622 

 N 21 23 23 22 

 of Female Pups MEAN 0.3389 0.3753 0.3731 0.3582 

 S.D. .06696 .07188 .05396 .04127 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
11-JAN-2010 13:28

Huntingdon Life Sciences 00-4205 PAGE 114 

 

 TABLE 22 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP ORGAN WEIGHT DATA 

 PUP ORGAN WEIGHT TO BODY WEIGHT RATIO 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

BRAIN MEAN 2.0057 2.0114 2.0624 2.0439 

 S.D. .18098 .21163 .23822 .14768 

 N 21 23 23 22 

 of Male Pups MEAN 1.9699 1.9982 2.0421 2.0405 

 S.D. .20062 .27934 .35601 .20930 

 N 21 23 23 22 

 of Female Pups MEAN 2.0415 2.0246 2.0826 2.0473 

 S.D. .19226 .18312 .17288 .14212 

 N 21 23 23 22 

SPLEEN MEAN 0.4148 0.3986 0.4143 0.3949 

 S.D. .03911 .03784 .04467 .04883 

 N 21 23 23 22 

 of Male Pups MEAN 0.4234 0.4055 0.4315 0.4021 

 S.D. .04498 .05447 .05852 .05190 

 N 21 23 23 22 

 of Female Pups MEAN 0.4062 0.3917 0.3971 0.3876 

 S.D. .05675 .03284 .04849 .05680 

 N 21 23 23 22 

THYMUS MEAN 0.4314 0.4766* 0.4841** 0.4631 

 S.D. .06560 .05889 .04908 .05711 

 N 21 23 23 22 

 of Male Pups MEAN 0.4140 0.4631 0.4602 0.4422 

 S.D. .07853 .06406 .07189 .07941 

 N 21 23 23 22 

 of Female Pups MEAN 0.4487 0.4902 0.5080** 0.4840 

 S.D. .07897 .06840 .04737 .04990 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.01 
11-JAN-2010 13:29

Huntingdon Life Sciences 00-4205 PAGE 115 

 

 TABLE 22 F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 SUMMARY OF PUP ORGAN WEIGHT DATA 

 PUP ORGAN WEIGHT TO BRAIN WEIGHT RATIO 

-----------------------------------------------------------------------------------------------------------------------------------

 DOSE GROUP I II III IV 

 DOSE LEVEL (MG/M3) 0 2000 10000 20000 

-----------------------------------------------------------------------------------------------------------------------------------

SPLEEN MEAN 20.926 20.019 20.477 19.496 

 S.D. 2.8883 2.6336 3.3055 2.6867 

 N 21 23 23 22 

 of Male Pups MEAN 21.780 20.568 21.717 19.976 

 S.D. 3.7017 3.5423 4.4926 3.6668 

 N 21 23 23 22 

 of Female Pups MEAN 20.071 19.469 19.236 19.015 

 S.D. 3.3357 2.1427 3.0478 3.0321 

 N 21 23 23 22 

THYMUS MEAN 21.702 23.991 23.922 22.881 

 S.D. 3.6459 3.9452 3.7068 3.4589 

 N 21 23 23 22 

 of Male Pups MEAN 21.231 23.537 23.291 22.044 

 S.D. 4.6190 4.3796 5.3658 5.4792 

 N 21 23 23 22 

 of Female Pups MEAN 22.174 24.446 24.553 23.718 

 S.D. 4.2181 4.3078 3.0137 2.6346 

 N 21 23 23 22 

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences 
Huntingdon Life Sciences 00-4205 Page 116

211-DIPE-1G Final Report

Lesion Incidence Summary

with Expanded Severity Levels

Preface Table 23

Key to Abbreviations

Gl = Gland

NAD = No abnormal diagnoses

Ctls = Controls (Group 1)

Oviducts/Fallop = Oviducts and Fallopian Tubes

Corresponding exposure levels for each group were as follows:

Group 1 - 0 mg/m3

Group 2 - 2,000 mg/m3 

Group 3 - 10,000 mg/m3

Group 4 - 20,000 mg/m3




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TABLE OF CONTENTS

1. INTRODUCTION..............................................................................................122

2. MATERIALS AND METHODS

2.1. Husbandry During Exposure Periods .......................................................122 

2.2. Test Substance Administration and Chamber Operation .........................123 

2.3. Exposure Concentration Determination ...................................................127 

3. TABLES

I. Summary of In-Chamber Observations ...................................................129 

II. Chamber Monitoring Records ..................................................................137 

III. Equipment List .........................................................................................162 

IV. Chamber Distribution Results ..................................................................165 

V. Miran Calibration .....................................................................................167 

VI. Testing Room and Chambers Environmental Monitoring .......................170 

4. FIGURES

I. Chamber Generation System and Whole-Body Exposure 

Chamber – Group I ..............................................................................171 

II. Chamber Generation System and Whole-Body Exposure 

Chamber – Groups II, III, IV ...............................................................172 
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1. INTRODUCTION

This appendix presents the methodology for exposure atmosphere generation 

monitoring and results. 

2. MATERIALS AND METHODS 

2.1. HUSBANDRY DURING EXPOSURE PERIODS

2.1.1. HOUSING

Animals were individually housed in stainless steel, wire mesh 

cages within a 1.5 m3 glass and stainless steel whole-body 

exposure chamber. The placement of the animals in the wholebody exposure chamber was rotated weekly to ensure uniform 

exposure of the animals. A description of the animal rotation is 

included in the raw data. 

2.1.2. FEED 

None was provided during exposure. 

2.1.3. WATER

None was provided during exposure. 

2.1.4. ENVIRONMENTAL CONDITIONS

Chamber temperature and relative humidity were recorded every 

half-hour during exposure and maintained, to the maximum extent 

possible, within the ranges presented below. Excursions outside 

the specified range did not affect the integrity of the study. 

Temperature

Desired: 20 to 24°C 

Actual: 21 to 28°C 
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The daily average temperature within the chamber was outside the 

desired range on 10/125 days, 105/126 days, 106/125 days and 

103/127 days for Groups 1, 2, 3 and 4, respectively. 

Relative Humidity

Desired: 40 to 60% 

Actual: 35 to 65% 

The daily average relative humidity within the chamber was 

outside the desired range on 3/125 days, 10/126 days, 6/125 days 

and 4/127 days for Groups 1, 2, 3 and 4, respectively. 

2.2. TEST SUBSTANCE ADMINISTRATION AND CHAMBER 

OPERATIONS 

2.2.1. ROUTE OF ADMINISTRATION

Inhalation via whole-body exposures 

2.2.2. TEST SUBSTANCE ADMINISTRATION

The test substance was administered as a vapor in the breathing air 

of the animals. The test atmosphere was generated by an 

appropriate procedure determined during the pre-study trials. The 

trials were performed to evaluate the optimal set of conditions and 

equipment to generate a stable atmosphere at the target exposure 

levels and maintain uniform conditions throughout the exposure 

chambers.

2.2.3. JUSTIFICATION FOR ROUTE OF ADMINISTRATION 

The inhalation route is one of the potential routes of human 

exposure to this test substance and is the route specified in the 

referenced US EPA 79.61 guidelines. 
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2.2.4. TARGET EXPOSURE LEVELS

Group I - 0 mg/m3

Group II - 2,000 mg/m3

Group III - 10,000 mg/m3

Group IV - 20,000 mg/m3

2.2.5. JUSTIFICATION FOR EXPOSURE LEVEL SELECTION

Exposure levels were selected by the Sponsor, based on results 

from a 13-week inhalation study conducted at the Testing Facility 

that utilized this test substance in rats (00-6130). The exposure 

levels were also selected based on the lower flammable limits of 

the test substance.

2.2.6. FREQUENCY OF ADMINISTRATION

Frequency 

P0 males and females received 70 consecutive days (ten weeks) of 

exposure prior to mating for six hours/day. P0 males and females 

continued to be exposed daily throughout a 14-day cohabitation 

period. The mated females continued to be exposed daily from 

Day 0 through 19 of gestation. Beginning on Day 5 of lactation, 

nursing P0 females were exposed daily until weaning of the F1

offspring on Day 28. P0 females with no confirmed day of mating 

but with evidence of pregnancy continued exposure until presumed 

Day 19 of gestation. P0 females with no confirmed day of mating 

and with no evidence of pregnancy (weight gain) continued 

exposure for 25 days following completion of the mating period 

and then euthanized. P0 females with a confirmed day of mating 

that did not deliver were euthanized on presumed Day 25 of 

gestation.

P0 males were exposed daily until euthanasia, which occurred after 

the date that the last F1 litters were delivered.
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2.2.7. DURATION 

The test substance was administered for 6 hours/day during all 

segments of the study. 

2.2.8. EXPOSURE PROCEDURE AND CHAMBER OPERATIONS 

Group I

Houseline nitrogen was delivered from a regulator with a 

backpressure gauge via 1⁄4” tubing to a flowmeter regulated by a 

metering valve. This nitrogen flow (~ 20 Lpm) was then directed 

into the turret of the 1.5 m3 glass and stainless steel exposure 

chamber where it was mixed with room air as it was drawn into the 

chamber. This nitrogen flow simulated the generation nitrogen 

flow for Groups II, III, and IV. 

Groups II, III and IV

Houseline nitrogen was delivered from a regulator with a 

backpressure gauge through a stainless steel fitting to create three 

flow systems: the test substance pressurization flow, the purge flow 

and the volatilization flow.

The nitrogen for the test substance pressurization flow was directed 

via 1⁄4” tubing through a metering valve, attached to a backpressure 

gauge, into the vapor inlet valve of the test substance cylinder. The 

metering valve was used to adjust and maintain the pressure within 

the cylinder. From the pressurized cylinder, the test substance 

flowed from the liquid outlet valve through a quick-disconnect 

fitting and through a filter to prevent equipment contamination. 

From the filter, the test substance flowed to a liquid flowmeter via 

1

/8” tubing. The outlet of the flowmeter was regulated by a built-in 

metering valve. From this metering valve, the test substance 

flowed via 1/8” tubing onto the glass helix of a counter current 

volatilization chamber. The glass helix was heated by a nichrome 

wire, which was controlled by an autotransformer and was inserted 

in the center of the glass tube that supported the helix. 
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The nitrogen for the purge flow system was directed, via 1⁄4” tubing 

to a flowmeter regulated by a metering valve. The purge nitrogen 

was delivered via 1/8” tubing to the bottom of the tube containing 

the nichrome wire. This nitrogen flow continuously purged the 

area surrounding the nichrome wire within the tube, thereby 

protecting the wire from oxidation. 

The nitrogen for the volatilization system was directed via 1⁄4” 

tubing to a flowmeter regulated by a metering valve. From the 

flowmeter, the volatilization nitrogen (~ 20 Lpm) flowed via 1⁄4” 

tubing to a ball and socket joint at the bottom of the volatilization 

chamber. This nitrogen flowed up through the volatilization 

chamber passing over the coil and volatilizing the test substance. 

The pressure within the counter-current volatilization chamber was 

maintained slightly negative to the room and was monitored with a 

pressure gauge.

This test substance laden nitrogen exited the top of the 

volatilization chamber through a glass elbow, which directed the 

flow, via 1⁄2” tubing, to the turret of 1.5 m3 glass and stainless steel 

exposure chamber. As the test substance laden nitrogen was drawn 

into the chamber, it was mixed with room air.

The whole-body exposure chambers each had a volume of 

approximately 1500 Liters (1.5 m3). Each chamber was operated at 

a minimum flow rate of 300 Liters per minute. The final airflow 

was set to provide at least one air change (calculated by dividing 

the chamber volume by the airflow rate) in 5.0 minutes (12 air 

changes/hour) and a T99 equilibrium time (calculated by 

multiplying the air change by the exponential factor 4.6) of at most 

23 minutes: 

Group Airflow Rate Air Change T99

(Lpm) (min) (min)

I 314 4.8 22

II 303 5.0 23

III 303 5.0 23

IV 312 4.8 22
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This chamber size and airflow rate was considered adequate to 

maintain the oxygen level at least 19% and the animal-loading 

factor below 5%. Chamber temperature, humidity, airflow rate and 

static pressure were monitored continuously and recorded every 

30 minutes during exposure. At the end of the 6-hour exposure, all 

animals remained in the chamber for a minimum of 30 minutes. 

During this time the chamber was operated at approximately the 

same flow rate using clean air only. The chambers were exhausted 

through the in house filtering system, which consisted of a coarse 

filter, a HEPA filter and activated charcoal.

See Chamber Figures I and II and Table III for equipment details.

2.3. EXPOSURE CONCENTRATION DETERMINATION

2.3.1. NOMINAL CONCENTRATION 

A nominal exposure concentration was calculated. The flow of air 

through the chamber was monitored using appropriate calibrated 

equipment. The test substance consumed (weight difference of the 

test substance cylinder) during the exposure (mg) was divided by 

the total volume of air (m3) passing through the chamber 

(volumetric flow rate times total exposure time) to give the 

nominal concentration. 

2.3.2. CHAMBER SAMPLING

During each exposure, measurements of airborne concentrations 

were performed in the animals’ breathing zone at least 4 times 

using an appropriate sampling procedure and Infra-red 

Spectrophotometric (IR) analytical method. Also, one charcoal 

tube sample per chamber per week was analyzed by gas 

chromatography (GC) to characterize at least 10 major components 

(comprising at least 80% by weight of the test substance) to show 

test substance stability and comparison between the neat liquid test 

substance and the vaporized test atmospheres.
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See Table III for equipment details. See Appendix B for Analytical 

Report. 

2.3.3. PARTICLE SIZE DISTRIBUTION 

During each week of exposure, particle size determinations were 

performed using a TSI Aerodynamic Particle Sizer to characterize 

the aerodynamic particle size distribution of any aerosol present. 

See Table III for equipment details.

2.3.4. CHAMBER AND EXPOSURE ROOM ENVIRONMENT

Chamber oxygen levels (maintained at least 19%) were measured 

pretest and at the beginning, middle, and end of the study. 

Air samples were taken in the vapor generation area pretest and at 

the beginning, middle and end of the study. Light (maintained 

approximately 30 foot-candles at 1.0 meters above the floor) and 

noise levels (maintained below 85 decibels) in the exposure room 

were measured pretest and at the beginning, middle and end of the 

study. 
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Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All All
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Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All All All
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Inhalation Report Appendix A

Table I

Summary of In-Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In-chamber observations are based on all animals present in the exposure chamber at the time.

Exposure Day 113 114 115 116 117 118 119 120 121 122 123 124 125 126

Group I – 0 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All - -

Group II – 2,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All -

Group III – 10,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All - -

Group IV – 20,000 mg/m3

Normal

Within Normal Limits All All All All All All All All All All All All All All
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Table II

Chamber Monitoring Records

Preface

Key to Abbreviations

MMAD = Mass Median Aerodynamic Diameter

GSD = Geometric Standard Deviation

TMC = Total Mass Concentration
























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Table III

Equipment List

Exposure Chamber

1500 Liter glass and stainless steel chamber (Unifab Cages).

Chamber Air-flow Gauges

Dwyer® Magnehelic® gauge (Dwyer Instruments Inc.), calibrated prestudy with 

a Side Trak III, equipped with a Digital Meter, Model 831-N2 (Sierra 

Instruments, Inc.).

Chamber Static Pressure

Dwyer® Magnehelic® gauge (Dwyer® Instruments Inc.); calibrated prestudy with a 

Dwyer Mark II Manometer, Model 25 (Dwyer Instruments Inc.).

Compound Generator

Counter-Current Volatilization Unit, coiled glass rod insert with nichrome wire 

(Crown Glass Co., Inc.).

Flowmeter with built-in valve, size 0-65 mm, tube #’s 6G02R3, 6G03R3, 6G04R3 

(Key Instruments). 

Variable Autotransformer, Type 3PN 1010 (Staco Energy Products Company).

T° Sentry Digital Alarm Module, Model 110 (Hampshire Controls Corp.).

Balston® Microfibre Disposable Filter Units, No. L9933-05 (Grade DQ).

Quick-disconnect fitting with toggle valve (Rego).

Compound Reservoir

5-gallon cylinder (Manchester Tank).

Balance

Mettler PM30000K (Mettler Instrument Corporation).

Pelooze, Model No. 4010.
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Table III

Equipment List

Flowmeters

Flowmeter, size 0-4, 0-10, 0-20, 0-40 Lpm (Dwyer® Instruments Inc.).

Top Trak Mass Flow Meter size 0-1 Lpm, Model 821-1 (Sierra Instruments), 

calibrated prestudy with a GilibratorBubble Generator, P/N D800286, S/N 

569-S, flow cell assembly P/N D800268, BD #1860).

Pressure/Vacuum Gauges

Ashcroft® backpressure gauge, P/N 733-47.

GAST® vacuum gauge, Model 169-01.

Matheson® backpressure gauge, P/N 63-3161.

Norgreen backpressure gauge, P/N 9892K23.

Dwyer® Magnehelic® gauge (Dwyer® Instruments Inc.).

Regulator

Norgreen, P/N 9892K23.

Valves

Metering Valve, Model SS-4L Series (Nupro® Co.).

Tubing

Plastic Size 1/2”, 1/4”, 3/16” (Norton); 1/8” (Tygon).

Teflon®

, size 1/8”, 1/4”, 1/2”.

Stainless steel 1/2”. 

T-Tube, stainless steel.

Glass elbow tube (Crown Glass Co.).

Stainless steel cross (Swage).

Filter

Balston® Microfibre Disposable Filter Units, Grade DQ.
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Table III

Equipment List

Air Analyzer

MIRAN® 1A-CVF Ambient Air Analyzer (Wilks) with a Cole Parmer strip recorder 

Model 201 and a Micronta® LCD Benchtop Digital Multimeter No. 22-195.

Oxygen/Gas Analyzer, Model 12145 (Gastech).

Digital Sound Level Meter, Model 840029 (Speer Scientific).

Photometer/Light Meter (Quantum Instruments).

Particle Sizer/Analyzer

TSI Aerodynamic Particle Sizer, Model 331001 and a DELL computer, Model 

486P/25, equipped with an Epson LQ-570+ Dot matrix printer, Model P630B.

Absorbent Tube Sampling

Charcoal Tubes, ORBO-32, Lot #2000 (Supelco).

Vacuum Pumps

Thomas Industries Inc., Model 707CM50, 107CA18 3.

Timer

Gralab Universal Timer, Model 171.

Environmental Monitoring

VWR Big Digit Temperature and Humidity Gauge, tested prestudy with a VWR 

NIST Traceable Digital Hygrometer/Thermometer.
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Table IV

Chamber Distribution Records

Group

(target)

Date Port IR Conc

(mg/m3)

Ratio to H-3

II (2,000 mg/m3) 17-May-02 H-3 1930 1.00

H-9 1910 0.99

H-3 1950 1.00

H-1 1890 0.97

H-2 1940 0.99

H-3 2080 1.00

H-8 2020 0.97

H-7 2100 1.01

18-May-02 H-3 2040 1.00

H-14 2090 1.02

H-13 1950 0.96

H-12 1870 0.92

H-11 1880 0.92

III (10,000 mg/m3) 17-May-02 H-3 10300 1.00

H-1 8390 0.81

H-2 9680 0.94

H-8 10200 0.99

H-3 9870 1.00

H-9 9680 0.98

H-7 10000 1.01

18-May-02 H-3 9940 1.00

H-14 10100 1.02

H-13 9610 0.97

H-12 9840 0.99

H-3 10700 1.00

H-11 10200 0.95

IV (20,000 mg/m3) 17-May-02 H-3 19300 1.00

H-9 19000 0.98

H-3 20300 1.05

H-1 20400 1.06

H-2 20200 1.05

H-7 20400 1.06
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Table IV

Chamber Distribution Records

Group

(target)

Date Port IR Conc

(mg/m3)

Ratio to H-3

IV (20,000 mg/m3) 17-May-02 H-3 20300 1.00

H-8 20100 0.99

18-May-02 H-3 20100 1.00

H-14 20200 1.00

H-13 20300 1.01

H-12 20400 1.02

H-3 19900 1.00

H-11 20000 1.01
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Table V

Miran Calibration

Methodology for Gasoline DIPE Vapor Condensate

Settings: The instrument settings for the unit are summarized below:

wavelength, microns 10.2

pathlength, dial setting 5.30

slit width, mm 1 

range, absorbance 1 

response, seconds 1 

gain High

chart speed, cm/min 1 

chart volts 1 

Calibrations: The Miran® was turned on and allowed to warm up for approximately 

10 minutes. The cell was flushed with room air for approximately one minute. The loop 

was closed, the unit was zeroed and the calibration series was performed as shown 

below. The resultant data were plotted to obtain a calibration curve. Each observer 

used a separate syringe for calibration.

Injection Calculated Absorbance2

Volume Concentration1 Operator 1 Operator 2 Average

(μL) (mg/m3) (volts) (volts) (volts)

8.4 953 0.0333 0.0325

0.0313 0.0304 0.0319

17 1929 0.0684 0.0711

0.0675 0.0698 0.0692

84 9532 0.319 0.321

0.315 0.316 0.318

170 19291 0.648 0.580

0.618 0.640 0.622

210 23830 0.788 0.838

0.770 0.760 0.789

1

Calculated Conc. (mg/m3) = Injection volume (μL) x Density (mg/μL) x 1000 L/m3

 5.64 L

where density = 0.64 mg/μL and 5.64 L = volume of Miran cell

2

Due to the variability of the initial results, two additional injections (one per operator) were 

performed for each concentration. All four values per concentration were used for the regression 

analysis.
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Table V

Miran Calibration

Calibration Checks: A three-point calibration check of the Miran® was 

performed for each exposure prior to sampling the chambers. The parameters 

are shown below:

Expected Acceptable

Injection Calculated Absorbance Absorbance

Volume Concentration Reading Range

(μL) (mg/m3) (volts) (volts)

17 1929 0.0692 0.0588 – 0.0796

84 9532 0.318 0.2703 – 0.366

170 19291 0.622 0.529 – 0.715

The absorbance was recorded after each injection. The absorbance was 

considered satisfactory if it was within 15% of the original calibration series. If 

any of the absorbance values fell outside the 15% range, the injection was 

rechecked as follows. The volume for the value that was out of range was 

reinjected twice. The closer pair of the three injections were averaged and the 

results were compared to the original curve. If the average of the pair was within 

the 15% range, the original was accepted. If the value of the average was 

outside the 15% range, the Study Director decided if a new graph was to be 

prepared.
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Table V

Miran Calibration

Calibration Curve for Gasoline DIPE Vapor Condensate

y = -40124x4

 + 41398x3 - 6533.9x2 + 29104x

R2

 = 0.9977

0

5000

10000

15000

20000

25000

30000

0.0000 0.2000 0.4000 0.6000 0.8000 1.0000

Absorbance (Volts)
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Appendix A

Table VI

Testing Room and Chambers Environmental Monitoring

Interval Location

Testing 

Room

Test Substance 

(mg/m3)

Testing 

Room 

Light

(Ft Candles)

Testing 

Room 

Noise

(dB)

Chamber 

Oxygen

(%)

Pretest Room 808 0 39.4 60.3 -

Group I - - - 20

Group II - - - 20

Group III - - - 20

Group IV - - - 20

Week 0 Room 808 0 35.0 62.5 -

Group I - - - 20

Group II - - - 20

Group III - - - 20

Group IV - - - 20

Week 9 Room 808 0 38.1 61.1 -

Group I - - - 20

Group II - - - 20

Group III - - - 20

Group IV - - - 20

Week 18 Room 808 0 37.3 64.2 -

Group I - - - 20

Group II - - - 20

Group III - - - 20

Group IV - - - 20
Huntingdon Life Sciences 00-4205 Page 171

211-DIPE-1G Final Report

Group I Inhalation Report Appendix A

Figure I

Chamber Generation System 

and Whole-Body Exposure Chamber

Notes:

1. Sampling Ports H-11 (left-bottom), H-12 (left-top), H-13 (right-bottom) and 

H-14 (right-top) used for pretest distribution sampling, were located on the 

back wall of the chambers.

2. Animals where individually housed on five levels within the exposure 

chamber.
Huntingdon Life Sciences 00-4205 Page 172

211-DIPE-1G Final Report

Groups II, III 

and IV Inhalation Report Appendix A

Figure II

Chamber Generation System 

and Whole-Body Exposure Chamber

Notes:

1. Sampling Ports H-11 (left-bottom), H-12 (left-top), H-13 (right-bottom) and 

H-14 (right-top) used for pretest distribution sampling, were located on the 

back wall of the chambers.

2. Animals where individually housed on five levels within the exposure 

chamber.






























11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 203 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 TERMINAL SACRIFICE 10-SEP-02 113 

 1027 TERMINAL SACRIFICE 10-SEP-02 113 

 1028 TERMINAL SACRIFICE 10-SEP-02 113 

 1029 TERMINAL SACRIFICE 10-SEP-02 113 

 1030 TERMINAL SACRIFICE 10-SEP-02 113 

 1031 TERMINAL SACRIFICE 10-SEP-02 113 

 1032 TERMINAL SACRIFICE 10-SEP-02 113 

 1033 TERMINAL SACRIFICE 10-SEP-02 113 

 1034 TERMINAL SACRIFICE 10-SEP-02 113 

 1035 TERMINAL SACRIFICE 10-SEP-02 113 

 1036 TERMINAL SACRIFICE 10-SEP-02 113 

 1037 TERMINAL SACRIFICE 10-SEP-02 113 

 1038 TERMINAL SACRIFICE 10-SEP-02 113 

 1039 TERMINAL SACRIFICE 11-SEP-02 114 

 1040 TERMINAL SACRIFICE 11-SEP-02 114 

 1041 TERMINAL SACRIFICE 11-SEP-02 114 

 1042 TERMINAL SACRIFICE 11-SEP-02 114 

 1043 TERMINAL SACRIFICE 11-SEP-02 114 

 1044 TERMINAL SACRIFICE 11-SEP-02 114 

 1045 TERMINAL SACRIFICE 11-SEP-02 114 

 1046 TERMINAL SACRIFICE 11-SEP-02 114 

 1047 TERMINAL SACRIFICE 11-SEP-02 114 

 1048 TERMINAL SACRIFICE 11-SEP-02 114 

 1049 TERMINAL SACRIFICE 11-SEP-02 114 

 1050 TERMINAL SACRIFICE 11-SEP-02 114 

 1051 TERMINAL SACRIFICE 11-SEP-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 16:15 

Huntingdon Life Sciences 00-4205 PAGE 204 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 TERMINAL SACRIFICE 10-SEP-02 113 

 2027 TERMINAL SACRIFICE 10-SEP-02 113 

 2028 TERMINAL SACRIFICE 10-SEP-02 113 

 2029 TERMINAL SACRIFICE 10-SEP-02 113 

 2030 TERMINAL SACRIFICE 10-SEP-02 113 

 2031 TERMINAL SACRIFICE 10-SEP-02 113 

 2032 TERMINAL SACRIFICE 10-SEP-02 113 

 2033 TERMINAL SACRIFICE 10-SEP-02 113 

 2034 TERMINAL SACRIFICE 10-SEP-02 113 

 2035 TERMINAL SACRIFICE 10-SEP-02 113 

 2036 TERMINAL SACRIFICE 10-SEP-02 113 

 2037 TERMINAL SACRIFICE 10-SEP-02 113 

 2038 TERMINAL SACRIFICE 10-SEP-02 113 

 2039 TERMINAL SACRIFICE 11-SEP-02 114 

 2040 TERMINAL SACRIFICE 11-SEP-02 114 

 2041 TERMINAL SACRIFICE 11-SEP-02 114 

 2042 TERMINAL SACRIFICE 11-SEP-02 114 

 2043 TERMINAL SACRIFICE 11-SEP-02 114 

 2044 TERMINAL SACRIFICE 11-SEP-02 114 

 2045 TERMINAL SACRIFICE 11-SEP-02 114 

 2046 TERMINAL SACRIFICE 11-SEP-02 114 

 2047 TERMINAL SACRIFICE 11-SEP-02 114 

 2048 TERMINAL SACRIFICE 11-SEP-02 114 

 2049 TERMINAL SACRIFICE 11-SEP-02 114 

 2050 TERMINAL SACRIFICE 11-SEP-02 114 

 2051 TERMINAL SACRIFICE 11-SEP-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 205 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 TERMINAL SACRIFICE 10-SEP-02 113 

 3027 TERMINAL SACRIFICE 10-SEP-02 113 

 3028 TERMINAL SACRIFICE 10-SEP-02 113 

 3029 TERMINAL SACRIFICE 10-SEP-02 113 

 3030 TERMINAL SACRIFICE 10-SEP-02 113 

 3031 TERMINAL SACRIFICE 10-SEP-02 113 

 3032 TERMINAL SACRIFICE 10-SEP-02 113 

 3033 TERMINAL SACRIFICE 10-SEP-02 113 

 3034 TERMINAL SACRIFICE 10-SEP-02 113 

 3035 TERMINAL SACRIFICE 10-SEP-02 113 

 3036 TERMINAL SACRIFICE 10-SEP-02 113 

 3037 TERMINAL SACRIFICE 10-SEP-02 113 

 3038 TERMINAL SACRIFICE 10-SEP-02 113 

 3039 TERMINAL SACRIFICE 11-SEP-02 114 

 3040 TERMINAL SACRIFICE 11-SEP-02 114 

 3041 TERMINAL SACRIFICE 11-SEP-02 114 

 3042 TERMINAL SACRIFICE 11-SEP-02 114 

 3043 HS HUMANE SACRIFICE 12-JUN-02 23 

 3044 DIED FOUND DEAD 7-SEP-02 110 

 3045 TERMINAL SACRIFICE 11-SEP-02 114 

 3046 TERMINAL SACRIFICE 11-SEP-02 114 

 3047 TERMINAL SACRIFICE 11-SEP-02 114 

 3048 TERMINAL SACRIFICE 11-SEP-02 114 

 3049 TERMINAL SACRIFICE 11-SEP-02 114 

 3050 TERMINAL SACRIFICE 11-SEP-02 114 

 3051 TERMINAL SACRIFICE 11-SEP-02 114 

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD HS=HUMANE SACRIFICE 
11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 206 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY 

ANIMAL# DEATH DEATH DAY 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 TERMINAL SACRIFICE 10-SEP-02 113 

 4027 TERMINAL SACRIFICE 10-SEP-02 113 

 4028 TERMINAL SACRIFICE 10-SEP-02 113 

 4029 TERMINAL SACRIFICE 10-SEP-02 113 

 4030 TERMINAL SACRIFICE 10-SEP-02 113 

 4031 TERMINAL SACRIFICE 10-SEP-02 113 

 4032 TERMINAL SACRIFICE 10-SEP-02 113 

 4033 TERMINAL SACRIFICE 10-SEP-02 113 

 4034 TERMINAL SACRIFICE 10-SEP-02 113 

 4035 TERMINAL SACRIFICE 10-SEP-02 113 

 4036 TERMINAL SACRIFICE 10-SEP-02 113 

 4037 TERMINAL SACRIFICE 10-SEP-02 113 

 4038 TERMINAL SACRIFICE 10-SEP-02 113 

 4039 TERMINAL SACRIFICE 11-SEP-02 114 

 4040 TERMINAL SACRIFICE 11-SEP-02 114 

 4041 TERMINAL SACRIFICE 11-SEP-02 114 

 4042 TERMINAL SACRIFICE 11-SEP-02 114 

 4043 TERMINAL SACRIFICE 11-SEP-02 114 

 4044 TERMINAL SACRIFICE 11-SEP-02 114 

 4045 TERMINAL SACRIFICE 11-SEP-02 114 

 4046 TERMINAL SACRIFICE 11-SEP-02 114 

 4047 TERMINAL SACRIFICE 11-SEP-02 114 

 4048 TERMINAL SACRIFICE 11-SEP-02 114 

 4049 TERMINAL SACRIFICE 11-SEP-02 114 

 4050 TERMINAL SACRIFICE 11-SEP-02 114 

 4051 TERMINAL SACRIFICE 11-SEP-02 114 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 207 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 TERMINAL SACRIFICE 21-SEP-02 124 P 

 1527 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1528 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1529 TERMINAL SACRIFICE 19-SEP-02 122 P 

 1530 TERMINAL SACRIFICE 20-SEP-02 123 P 

 1531 TERMINAL SACRIFICE 19-SEP-02 122 P 

 1532 TERMINAL SACRIFICE 17-SEP-02 120 P 

 1533 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1534 TERMINAL SACRIFICE 23-AUG-02 95 NP

 1535 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1536 TERMINAL SACRIFICE 17-SEP-02 120 P 

 1537 TERMINAL SACRIFICE 17-SEP-02 120 P 

 1538 TERMINAL SACRIFICE 26-AUG-02 98 NP

 1539 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1540 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 1541 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1542 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1543 TERMINAL SACRIFICE 19-SEP-02 122 P 

 1544 TERMINAL SACRIFICE 20-SEP-02 123 P 

 1545 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1546 TERMINAL SACRIFICE 17-SEP-02 120 P 

 1547 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1548 TERMINAL SACRIFICE 24-AUG-02 96 NP

 1549 TLL TERMINAL SACRIFICE 22-SEP-02 125 P 

 1550 TERMINAL SACRIFICE 18-SEP-02 121 P 

 1551 TERMINAL SACRIFICE 18-SEP-02 121 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS 
11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 208 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2527 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2528 TERMINAL SACRIFICE 18-SEP-02 121 P 

 2529 TERMINAL SACRIFICE 17-SEP-02 120 P 

 2530 TERMINAL SACRIFICE 17-SEP-02 120 P 

 2531 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2532 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2533 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2534 TERMINAL SACRIFICE 18-SEP-02 121 P 

 2535 TERMINAL SACRIFICE 20-SEP-02 123 P 

 2536 HS HUMANE SACRIFICE 6-AUG-02 78 P 

 2537 TERMINAL SACRIFICE 20-SEP-02 123 P 

 2538 TERMINAL SACRIFICE 20-SEP-02 123 P 

 2539 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2540 TERMINAL SACRIFICE 20-SEP-02 123 P 

 2541 TERMINAL SACRIFICE 20-SEP-02 123 P 

 2542 TERMINAL SACRIFICE 18-SEP-02 121 P 

 2543 PNMD TERMINAL SACRIFICE 23-SEP-02 126 P 

 2544 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 2545 TERMINAL SACRIFICE 20-SEP-02 123 P 

 2546 TERMINAL SACRIFICE 19-SEP-02 122 P 

 2547 TERMINAL SACRIFICE 17-SEP-02 120 P 

 2548 TERMINAL SACRIFICE 17-SEP-02 120 P 

 2549 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 2550 TERMINAL SACRIFICE 18-SEP-02 121 P 

 2551 TERMINAL SACRIFICE 20-SEP-02 123 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 
11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 209 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 3527 TERMINAL SACRIFICE 20-SEP-02 123 P 

 3528 TERMINAL SACRIFICE 19-SEP-02 122 P 

 3529 TERMINAL SACRIFICE 19-SEP-02 122 P 

 3530 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 3531 TERMINAL SACRIFICE 18-SEP-02 121 P 

 3532 TERMINAL SACRIFICE 19-SEP-02 122 P 

 3533 TERMINAL SACRIFICE 19-SEP-02 122 P 

 3534 TLL TERMINAL SACRIFICE 22-SEP-02 125 P 

 3535 TERMINAL SACRIFICE 17-SEP-02 120 P 

 3536 TERMINAL SACRIFICE 18-SEP-02 121 P 

 3537 TERMINAL SACRIFICE 22-SEP-02 125 P 

 3538 TERMINAL SACRIFICE 21-SEP-02 124 P 

 3539 TERMINAL SACRIFICE 21-SEP-02 124 P 

 3540 TERMINAL SACRIFICE 17-SEP-02 120 P 

 3541 TERMINAL SACRIFICE 18-SEP-02 121 P 

 3542 TERMINAL SACRIFICE 20-SEP-02 123 P 

 3543 TERMINAL SACRIFICE 20-SEP-02 123 P 

 3544 TERMINAL SACRIFICE 19-SEP-02 122 P 

 3545 TERMINAL SACRIFICE 18-SEP-02 121 P 

 3546 TERMINAL SACRIFICE 20-SEP-02 123 P 

 3547 TERMINAL SACRIFICE 17-SEP-02 120 P 

 3548 TERMINAL SACRIFICE 20-SEP-02 123 P 

 3549 TERMINAL SACRIFICE 20-SEP-02 123 P 

 3550 TERMINAL SACRIFICE 18-SEP-02 121 P 

 3551 TERMINAL SACRIFICE 20-SEP-02 123 P 

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS 
11-JAN-2010 16:15

Huntingdon Life Sciences 00-4205 PAGE 210 

 

 APPENDIX C P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ANIMAL TERMINATION HISTORY 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 TERMINAL SACRIFICE 18-SEP-02 121 P 

 4527 TERMINAL SACRIFICE 24-SEP-02 127 P 

 4528 TERMINAL SACRIFICE 18-SEP-02 121 P 

 4529 TERMINAL SACRIFICE 18-SEP-02 121 P 

 4530 PNMD TERMINAL SACRIFICE 19-SEP-02 122 P 

 4531 TERMINAL SACRIFICE 17-SEP-02 120 P 

 4532 TERMINAL SACRIFICE 20-SEP-02 123 P 

 4533 TERMINAL SACRIFICE 19-SEP-02 122 P 

 4534 TERMINAL SACRIFICE 20-SEP-02 123 P 

 4535 TERMINAL SACRIFICE 19-SEP-02 122 P 

 4536 TERMINAL SACRIFICE 18-SEP-02 121 P 

 4537 TERMINAL SACRIFICE 19-SEP-02 122 P 

 4538 HS HUMANE SACRIFICE 3-SEP-02 106 P 

 4539 TERMINAL SACRIFICE 19-SEP-02 122 P 

 4540 TERMINAL SACRIFICE 18-SEP-02 121 P 

 4541 TERMINAL SACRIFICE 20-SEP-02 123 P 

 4542 TERMINAL SACRIFICE 23-SEP-02 126 P 

 4543 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 4544 NNMD TERMINAL SACRIFICE 5-SEP-02 108 NP

 4545 TERMINAL SACRIFICE 20-SEP-02 123 P 

 4546 TERMINAL SACRIFICE 19-SEP-02 122 P 

 4547 TERMINAL SACRIFICE 18-SEP-02 121 P 

 4548 TERMINAL SACRIFICE 17-SEP-02 120 P 

 4549 TERMINAL SACRIFICE 21-SEP-02 124 P 

 4550 TERMINAL SACRIFICE 19-SEP-02 122 P 

 4551 TERMINAL SACRIFICE 5-SEP-02 108 NP

-----------------------------------------------------------------------------------------------------------------------------------

NP-NOT PREGNANT, P-PREGNANT

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 
Huntingdon Life Sciences 00-4205 Page 211

211-DIPE-1G Final Report

Individual Clinical Observations

Preface Appendix D

For summarization purposes, descriptive comments [i.e., location of scab(s) and 

sore(s), etc.] are not presented in this appendix. These data are contained in the 

study raw data if needed.

Deaths occurring noted between scheduled weekly examinations are presented 

at the previous interval. The actual day of an animal’s death is presented in 

Appendix C.

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m3 

Group II - 2,000 mg/m3

Group III - 10,000 mg/m3

Group IV - 20,000 mg/m3 
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 212 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1029 WITHIN NORMAL LIMITS P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3 3 3 3 2 

 ALOPECIA - GENERAL 2 2 

 1030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1033 WITHIN NORMAL LIMITS P P P P P P P P P 

 SWOLLEN PAW(S) P P P 

 RIGHT FORE PAW 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 

 CHROMODACRYORRHEA - BILATERAL P P 

 CHROMODACRYORRHEA - UNILATERAL P P 

 INCISORS BROKEN/MISSING P P P P P P P P 

 1034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P 

 LACRIMATION - UNILATERAL 2 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 213 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 1036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P P P 

 1039 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P 

 INCISORS MALOCCLUDED P P P P P P P 

 1040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1042 WITHIN NORMAL LIMITS P P P P P P P P P 

 SWOLLEN DIGIT P P P P P P P P P 

 FIRST DIGIT LEFT FORE PAW 

 1043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1045 WITHIN NORMAL LIMITS P P 

 ULCERATION - CERVICAL P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 3 3 

 SCABS P P 

 CERVICAL AREA 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 214 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 1046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P 

 1047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 1051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 215 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P 

 2027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 2036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 216 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 2040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 

 2042 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P 

 2044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2050 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 

 2051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 217 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 WITHIN NORMAL LIMITS P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2 

 3027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P 

 INCISORS MALOCCLUDED P P P P 

 3028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3029 WITHIN NORMAL LIMITS P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 3 3 3 3 3 3 2 2 

 3030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P 

 3032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P P 

 3036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3037 WITHIN NORMAL LIMITS P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2 3 3 3 3 3 3 3 3 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 218 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 3038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 

 3042 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P 

 INCISORS BROKEN/MISSING P P P P P P P 

 3043 WITHIN NORMAL LIMITS P P P P 

 HUMANE SACRIFICE P 

 CHROMODACRYORRHEA - BILATERAL P 

 ORAL SORE P 

 3044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 FOUND DEAD P 

 3045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 219 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 3049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 3051 WITHIN NORMAL LIMITS P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 3 3 3 3 3 3 3 3 3 

 ALOPECIA - GENERAL 2 2 2 2 2 2 2 2 3 3 3 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 220 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 

 4029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 

 INCISORS BROKEN/MISSING P 

 INCISORS MALOCCLUDED P 

 4031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P 

 SCABS P P P P 

 CERVICAL 

 4033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4035 WITHIN NORMAL LIMITS P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 3 3 

 CHROMODACRYORRHEA - BILATERAL P P P P P P P 

 INCISORS MALOCCLUDED P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 221 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 4036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4039 WITHIN NORMAL LIMITS P P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P P P P P P P P P 

 LACRIMATION - UNILATERAL 2 

 INCISORS BROKEN/MISSING P P P P P 

 INCISORS MALOCCLUDED P P P P P P P P P 

 4040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4042 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4045 WITHIN NORMAL LIMITS P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P P 

 INCISORS BROKEN/MISSING P P P P P P P P P 

 INCISORS MALOCCLUDED P P P P P P P P P P P 

 4046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:32

Huntingdon Life Sciences 00-4205 PAGE 222 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 4047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4048 WITHIN NORMAL LIMITS P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 2 2 2 2 3 3 3 

 4049 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P P P P P P P 

 4050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 4051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 223 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1527 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 INCISORS BROKEN/MISSING P 

 1528 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1529 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1530 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1531 WITHIN NORMAL LIMITS P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 1532 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1533 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1534 WITHIN NORMAL LIMITS P P P P P P P P P 

 SWOLLEN DIGIT P 

 RIGHT FORE PAW 

 SWOLLEN DIGIT P 

 THIRD DIGIT RIGHT FORE PAW 

 1535 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1536 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 1537 WITHIN NORMAL LIMITS P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 224 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 1538 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1539 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1540 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 1541 WITHIN NORMAL LIMITS P P P P P P P 

 CHROMODACRYORRHEA - UNILATERAL P P P P P 

 LACRIMATION - UNILATERAL 3 3 3 3 

 INCISORS BROKEN/MISSING P P P P P 

 1542 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1543 WITHIN NORMAL LIMITS P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3 

 INCISORS MALOCCLUDED P 

 1544 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1545 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1546 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 1547 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1548 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1549 WITHIN NORMAL LIMITS P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 225 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 1550 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 1551 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 226 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2527 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2528 WITHIN NORMAL LIMITS P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 2529 WITHIN NORMAL LIMITS P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 

 2530 WITHIN NORMAL LIMITS P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 3 

 INCISORS MALOCCLUDED P 

 2531 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2532 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2533 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2534 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 SWOLLEN DIGIT P 

 FOURTH DIGIT RIGHT FORE PAW 

 2535 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2536 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2537 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 227 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 2538 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2539 WITHIN NORMAL LIMITS P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 3 3 

 2540 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2541 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2542 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2543 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P 

 2544 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 2545 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2546 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2547 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 2548 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 2549 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 2550 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 228 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 2551 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 229 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 3527 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3528 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3529 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3530 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 INCISORS BROKEN/MISSING P 

 3531 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3532 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3533 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3534 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3535 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 3536 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3537 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 

 3538 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 230 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 3539 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3540 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 3541 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3542 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3543 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3544 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3545 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3546 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3547 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 3548 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3549 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3550 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 3551 WITHIN NORMAL LIMITS P P P P P P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 

 ALOPECIA - GENERAL 2 2 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 231 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4527 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4528 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 ALOPECIA - GENERAL 2 

 4529 WITHIN NORMAL LIMITS P P P P P P P P P P 

 INCISORS BROKEN/MISSING P 

 INCISORS MALOCCLUDED P P 

 4530 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 4531 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 4532 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4533 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4534 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4535 WITHIN NORMAL LIMITS P P P P P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3 3 

 4536 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4537 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4538 WITHIN NORMAL LIMITS P P P P P P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:33

Huntingdon Life Sciences 00-4205 PAGE 232 

 

 APPENDIX D P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 1 1 

 DAY OF - 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1 

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2 

-----------------------------------------------------------------------------------------------------------------------------------

 4539 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4540 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4541 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4542 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4543 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 4544 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

 4545 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4546 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4547 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4548 WITHIN NORMAL LIMITS P P P P P P P P P P P 

 4549 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4550 WITHIN NORMAL LIMITS P P P P P P P P P P P P 

 4551 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P 

 TERMINAL SACRIFICE P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 233 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 WITHIN NORMAL LIMITS P P P P 

 1527 WITHIN NORMAL LIMITS P P P P 

 1528 WITHIN NORMAL LIMITS P P P P 

 1529 WITHIN NORMAL LIMITS P P P P 

 1530 WITHIN NORMAL LIMITS P P P P 

 1531 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 ALOPECIA - GENERAL 2 2 2 

 1532 WITHIN NORMAL LIMITS P P P P 

 1533 WITHIN NORMAL LIMITS P P P P 

 1534 TERMINAL SACRIFICE P 

 SWOLLEN DIGIT P P 

 MISSING DIGIT P P 

 1535 ALOPECIA - EXTREMITIES/SNOUT 2 

 WITHIN NORMAL LIMITS P P P 

 1536 WITHIN NORMAL LIMITS P P P P 

 1537 WITHIN NORMAL LIMITS P P P P 

 1538 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

 1539 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 234 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 1541 CHROMODACRYORRHEA - UNILATERAL P P P P 

 INCISORS BROKEN/MISSING P P P P 

 1542 WITHIN NORMAL LIMITS P P P P 

 1543 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 2 

 1544 WITHIN NORMAL LIMITS P P P P 

 1545 WITHIN NORMAL LIMITS P P P P 

 1546 WITHIN NORMAL LIMITS P P P P 

 1547 WITHIN NORMAL LIMITS P P P P 

 1548 TERMINAL SACRIFICE P 

 WITHIN NORMAL LIMITS P P P P 

 1549 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 1550 WITHIN NORMAL LIMITS P P P P 

 1551 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 235 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 WITHIN NORMAL LIMITS P P P P 

 2527 WITHIN NORMAL LIMITS P P P P 

 2528 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 

 WITHIN NORMAL LIMITS P 

 2529 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 2530 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 

 2531 WITHIN NORMAL LIMITS P P P P 

 2532 WITHIN NORMAL LIMITS P P P P 

 2533 WITHIN NORMAL LIMITS P P P P 

 2534 WITHIN NORMAL LIMITS P P P P 

 2535 WITHIN NORMAL LIMITS P P P P 

 2536 HUMANE SACRIFICE P 

 CHROMODACRYORRHEA - BILATERAL P 

 INCISORS BROKEN/MISSING P 

 DECREASED FECAL VOLUME P 

 2537 WITHIN NORMAL LIMITS P P P P 

 2538 WITHIN NORMAL LIMITS P P P P 

 2539 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 236 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 2540 WITHIN NORMAL LIMITS P P P P 

 2541 WITHIN NORMAL LIMITS P P P P 

 2542 WITHIN NORMAL LIMITS P P P P 

 2545 WITHIN NORMAL LIMITS P P P P 

 2546 WITHIN NORMAL LIMITS P P P P 

 2547 WITHIN NORMAL LIMITS P P P P 

 2548 ALOPECIA - EXTREMITIES/SNOUT 2 

 ALOPECIA - GENERAL 2 2 2 2 

 2550 WITHIN NORMAL LIMITS P P P P 

 2551 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 237 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 WITHIN NORMAL LIMITS P P P P 

 3528 WITHIN NORMAL LIMITS P P P P 

 3529 WITHIN NORMAL LIMITS P P P P 

 3531 WITHIN NORMAL LIMITS P P P P 

 3532 WITHIN NORMAL LIMITS P P P P 

 3533 ALOPECIA - EXTREMITIES/SNOUT 2 

 WITHIN NORMAL LIMITS P P P 

 3534 WITHIN NORMAL LIMITS P P P P 

 3535 WITHIN NORMAL LIMITS P P P P 

 3536 WITHIN NORMAL LIMITS P P P P 

 3537 WITHIN NORMAL LIMITS P P P P 

 3538 WITHIN NORMAL LIMITS P P P P 

 3539 WITHIN NORMAL LIMITS P P P P 

 3540 WITHIN NORMAL LIMITS P P P P 

 3541 WITHIN NORMAL LIMITS P P P P 

 3542 WITHIN NORMAL LIMITS P P P P 

 3543 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 238 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 3544 WITHIN NORMAL LIMITS P P P P 

 3545 WITHIN NORMAL LIMITS P P P P 

 3546 WITHIN NORMAL LIMITS P P P P 

 3547 WITHIN NORMAL LIMITS P P P P 

 3548 WITHIN NORMAL LIMITS P P P P 

 3549 WITHIN NORMAL LIMITS P P P P 

 3550 WITHIN NORMAL LIMITS P P P P 

 3551 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 ALOPECIA - GENERAL 2 2 2 2 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 239 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 WITHIN NORMAL LIMITS P P P P 

 4527 WITHIN NORMAL LIMITS P P P P 

 4528 WITHIN NORMAL LIMITS P P P P 

 4529 INCISORS MALOCCLUDED P P P P 

 4531 WITHIN NORMAL LIMITS P P P P 

 4532 WITHIN NORMAL LIMITS P P P P 

 4533 WITHIN NORMAL LIMITS P P P P 

 4534 WITHIN NORMAL LIMITS P P P P 

 4535 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 

 ALOPECIA - GENERAL 3 3 3 

 4536 WITHIN NORMAL LIMITS P P P P 

 4537 WITHIN NORMAL LIMITS P P P P 

 4538 WITHIN NORMAL LIMITS P P P P 

 4539 WITHIN NORMAL LIMITS P P P P 

 4540 WITHIN NORMAL LIMITS P P P P 

 4541 WITHIN NORMAL LIMITS P P P P 

 4542 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 16:46

Huntingdon Life Sciences 00-4205 PAGE 240 

 

 APPENDIX E P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 

-----------------------------------------------------------------------------------------------------------------------------------

 4545 WITHIN NORMAL LIMITS P P P P 

 4546 WITHIN NORMAL LIMITS P P P P 

 4547 WITHIN NORMAL LIMITS P P P P 

 4548 WITHIN NORMAL LIMITS P P P P 

 4549 WITHIN NORMAL LIMITS P P P P 

 4550 WITHIN NORMAL LIMITS P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 241 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 1526 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1527 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1528 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1529 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1530 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1531 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 3 3 3

 ALOPECIA - GENERAL 2 2 2 3 3 3

 1532 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1533 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1535 WITHIN NORMAL LIMITS P P P

 TERMINAL SACRIFICE P

ALOPECIA - EXTREMITIES/SNOUT 2 2 

 YELLOW ANO-GENITAL STAINING 2 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 242 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 1536 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1537 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1539 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1541 TERMINAL SACRIFICE P

 CHROMODACRYORRHEA - UNILATERAL P P P P P P

 INCISORS BROKEN/MISSING P P P P P P

 1542 WITHIN NORMAL LIMITS P P P P P 

 TERMINAL SACRIFICE P

EMACIATION P 

 1543 TERMINAL SACRIFICE P

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3

 1544 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1545 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1546 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 1547 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 243 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 1549 ALOPECIA - EXTREMITIES/SNOUT 2 2 3 3 3 

 TERMINAL SACRIFICE P 

 1550 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 1551 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 244 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 2526 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 2527 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 2528 TERMINAL SACRIFICE P

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2

 2529 TERMINAL SACRIFICE P

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2

 2530 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3 

 2531 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2532 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2533 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2534 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2535 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 245 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 2537 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2538 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2539 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3

 2540 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2541 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2542 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2543 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2545 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2546 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 2547 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 246 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 2548 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 2

 ALOPECIA - GENERAL 2 2 2 2 2 2

 2550 WITHIN NORMAL LIMITS P P P P P

 TERMINAL SACRIFICE P

 INCISORS BROKEN/MISSING P 

 2551 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 247 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 3527 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3528 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3529 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3531 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3532 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3533 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 3

 3534 WITHIN NORMAL LIMITS P P P P P 

 TERMINAL SACRIFICE P 

 3535 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3536 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3537 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 3538 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 248 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 3539 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3540 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3541 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3542 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 3543 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 3544 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 3545 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 3546 WITHIN NORMAL LIMITS P 

 TERMINAL SACRIFICE P

 ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 2 

 3547 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 3548 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 249 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 3549 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 3550 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 3551 WITHIN NORMAL LIMITS P P

 TERMINAL SACRIFICE P

ALOPECIA - EXTREMITIES/SNOUT 2 2 2 2 

 ALOPECIA - GENERAL 2 2 2 2 

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 250 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 4526 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4527 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4528 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4529 TERMINAL SACRIFICE P

 INCISORS MALOCCLUDED P P P P P P

 4530 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4531 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4532 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4533 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4534 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4535 TERMINAL SACRIFICE P 

 ALOPECIA - EXTREMITIES/SNOUT 3 3 3 3 3 3

 ALOPECIA - GENERAL 3 3 3 3 3 3

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 251 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 4536 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 4537 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P

 4538 WITHIN NORMAL LIMITS P P P P 

 HUMANE SACRIFICE P 

INCISORS MALOCCLUDED P 

SWOLLEN SNOUT P 

RED EXUDATE SNOUT P 

 4539 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4540 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4541 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4542 WITHIN NORMAL LIMITS P P P P P P 

 TERMINAL SACRIFICE P 

 4545 WITHIN NORMAL LIMITS P P P P 

 TERMINAL SACRIFICE P 

ALOPECIA - EXTREMITIES/SNOUT 2 2 

 4546 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
12-JAN-2010 16:36

Huntingdon Life Sciences 00-4205 PAGE 252 

 

 APPENDIX F P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

--------------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------------

 4547 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4548 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4549 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

 4550 WITHIN NORMAL LIMITS P P P P P P

 TERMINAL SACRIFICE P

--------------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
Huntingdon Life Sciences 00-4205 Page 253

211-DIPE-1G Final Report

Individual Body Weights

(Premating/Mating/Postmating)

Preface Appendix G

No data is presented for the following animals at the stated intervals for the 

following reasons:

Animal No. Day(s) Reason

3043 28 to 112 Sacrificed for Humane Reasons on Day 23

3044 112 Found Dead on Day 110

1540 112 to 119 Terminal Sacrifice on Day 108

1549 77 to 91 Total Litter Loss on Lactation Day 2, therefore weekly 

body weights were resumed.

2543 98 to 119 Although no evidence of mating was observed, this 

female delivered on Day 98. See Lactation Body 

Weights.

2544 112 to 119 Terminal Sacrifice on Day 108

2549 112 to 119 Terminal Sacrifice on Day 108

3526 112 to 119 Terminal Sacrifice on Day 108

3530 112 to 119 Terminal Sacrifice on Day 108

3534 77 to 91 Total Litter Loss on Lactation Day 1, therefore weekly 

body weights were resumed.

4530 98 to 119 Although no evidence of mating was observed, this 

female delivered on Day 94. See Lactation Body 

Weights.

4543 112 to 119 Terminal Sacrifice on Day 108

4544 112 to 119 Terminal Sacrifice on Day 108

4551 112 to 119 Terminal Sacrifice on Day 108
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 254 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 1026 142 209 267 320 364 397 427 457 483 503 514 525 544 552 557 574 

 1027 151 217 268 325 364 408 442 468 485 501 522 523 538 553 557 568 

 1028 140 204 254 298 343 380 406 430 456 471 486 492 503 513 519 526 

 1029 135 195 249 295 330 366 393 419 434 454 472 474 476 488 491 501 

 1030 151 228 284 337 379 409 434 462 482 493 500 515 519 538 539 558 

 1031 147 210 266 322 365 384 419 434 449 474 489 501 500 492 506 523 

 1032 166 242 297 349 404 412 448 478 496 514 523 546 543 562 567 583 

 1033 143 225 274 320 352 392 427 449 469 493 474 467 487 514 525 535 

 1034 133 189 238 283 321 348 372 395 413 430 448 456 473 480 482 498 

 1035 139 204 248 292 335 358 388 413 431 442 455 467 474 495 496 507 

 1036 143 220 281 343 386 407 431 464 483 496 510 516 527 536 548 573 

 1037 133 199 251 300 336 361 387 410 425 432 452 466 468 480 482 494 

 1038 154 224 275 326 360 396 417 444 459 486 493 503 520 534 544 555 

 1039 132 197 241 291 329 359 388 420 425 447 447 455 466 474 487 493 

 1040 135 213 277 337 387 427 465 499 521 552 560 584 593 607 615 632 

 1041 148 221 282 352 404 450 491 516 539 545 567 597 613 623 630 649 

 1042 153 223 281 344 394 436 469 502 518 552 568 585 598 624 643 661 

 1043 130 192 240 282 309 318 335 352 373 381 392 405 409 411 414 428 

 1044 135 218 277 336 382 417 447 476 494 503 528 543 553 562 577 591 

 1045 164 226 275 319 361 382 408 430 446 466 475 495 501 518 524 532 

 1046 155 232 295 339 380 391 421 454 475 488 502 523 517 539 547 569 

 1047 141 197 243 290 339 365 398 412 438 452 462 478 475 486 491 497 

 1048 161 218 279 335 361 392 432 467 488 511 542 542 558 560 576 595 

 1049 148 216 268 324 360 395 423 451 466 489 516 527 540 556 568 580 

 1050 160 233 290 344 389 411 435 352 453 482 497 514 536 556 558 571 

 1051 137 199 252 289 327 364 384 398 415 427 443 449 487 469 472 485 

MEAN 145 213 267 319 360 389 419 440 462 480 494 506 516 528 535 549 

S.D. 10.4 14.1 17.6 22.6 26.6 29.4 33.4 40.8 37.8 40.3 42.2 45.5 46.4 49.7 52.0 54.4

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 255 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 2026 134 200 244 288 317 342 372 393 413 425 444 456 464 486 477 487 

 2027 141 208 253 296 335 363 391 415 429 445 454 467 478 485 493 498 

 2028 136 204 250 302 348 378 412 434 452 469 484 503 505 523 534 540 

 2029 147 217 272 327 378 412 448 473 492 507 527 534 538 556 557 560 

 2030 140 206 262 317 372 417 456 482 509 537 552 568 574 593 601 600 

 2031 140 205 259 307 345 374 401 424 434 448 466 469 474 491 493 499 

 2032 133 193 245 293 334 360 384 408 421 427 438 452 451 468 474 480 

 2033 130 211 276 336 387 416 450 479 500 521 542 564 570 593 605 612 

 2034 153 221 277 331 372 409 449 470 488 511 531 543 557 572 588 597 

 2035 144 209 260 304 336 360 371 397 405 426 441 447 455 475 479 489 

 2036 135 208 253 301 330 355 373 396 408 420 429 435 439 455 463 471 

 2037 154 219 268 322 362 389 423 456 479 492 509 522 526 543 547 553 

 2038 143 208 261 304 343 374 402 424 437 454 469 483 494 505 510 530 

 2039 152 214 263 314 354 383 418 434 457 469 491 506 522 531 544 553 

 2040 150 218 271 319 366 397 429 448 474 490 504 519 532 544 547 571 

 2041 163 230 288 338 383 412 444 475 492 514 525 541 550 562 577 592 

 2042 149 211 260 304 350 388 418 448 460 473 486 497 506 515 523 536 

 2043 161 223 278 330 379 414 453 484 514 529 555 560 574 595 597 614 

 2044 159 233 294 354 403 430 465 495 524 542 570 577 589 611 627 640 

 2045 166 237 301 364 405 443 485 519 541 561 578 594 600 607 622 642 

 2046 142 212 266 308 342 376 404 426 444 462 477 492 497 507 524 540 

 2047 133 200 253 307 351 387 416 444 468 485 509 515 533 551 554 568 

 2048 131 195 241 283 318 338 371 389 402 418 434 440 453 459 469 485 

 2049 155 226 288 346 392 440 483 523 555 569 599 614 625 641 647 666 

 2050 148 215 270 323 371 403 436 461 481 499 506 525 536 552 573 574 

 2051 137 192 231 272 316 345 379 398 422 438 451 471 488 499 508 516 

MEAN 145 212 265 315 357 389 420 446 465 482 499 511 520 535 543 554

S.D. 10.5 11.9 16.9 21.9 25.8 29.6 34.7 38.6 43.1 45.0 48.3 49.5 50.4 51.5 53.6 55.1 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 256 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 3026 133 203 256 308 352 396 424 454 469 494 507 516 521 532 547 552 

 3027 134 193 239 286 318 349 375 393 409 426 431 438 445 461 463 465 

 3028 154 217 275 329 373 413 451 476 501 524 539 556 560 579 582 593 

 3029 145 215 269 320 358 388 413 439 458 487 507 517 529 551 556 578 

 3030 141 216 280 342 393 436 474 498 517 548 552 576 577 594 596 609 

 3031 134 204 259 308 339 366 401 422 428 446 465 475 484 497 490 501 

 3032 142 198 248 291 327 354 376 396 420 438 448 462 470 476 487 482 

 3033 136 199 263 321 364 396 421 441 455 474 488 498 505 530 549 565 

 3034 149 216 271 329 366 391 430 456 467 493 504 519 531 543 559 579 

 3035 153 218 274 332 374 410 447 469 485 506 513 524 534 558 575 588 

 3036 130 193 243 295 330 358 382 398 413 433 435 435 452 464 466 473 

 3037 147 213 270 329 370 394 420 445 456 477 491 495 513 524 527 538 

 3038 140 200 259 310 349 374 401 425 449 470 473 491 495 501 515 523 

 3039 135 219 273 322 359 380 413 434 463 487 486 502 511 519 531 547 

 3040 137 216 277 337 384 420 458 486 509 526 544 554 571 591 603 622 

 3041 148 233 298 355 400 426 464 484 512 530 544 556 562 575 588 603 

 3042 157 228 288 343 380 404 429 440 462 476 490 495 499 509 510 523 

 3043 150 225 292 362 290 

 3044 154 212 268 302 335 359 383 399 423 448 463 476 483 502 504 513 

 3045 141 199 252 291 322 338 356 372 396 411 420 426 435 445 455 459 

 3046 156 224 276 317 345 370 392 420 425 437 453 460 469 479 485 499 

 3047 139 200 250 305 342 367 406 434 449 464 473 490 502 520 526 531 

 3048 132 191 243 289 336 363 388 411 431 457 466 476 485 505 514 526 

 3049 168 232 281 318 351 380 407 423 432 456 472 485 496 510 513 519 

 3050 162 232 296 359 405 439 469 502 526 558 571 592 605 611 616 630 

 3051 162 232 293 345 385 420 466 491 519 539 552 568 582 595 604 609 

MEAN 145 213 269 321 356 388 418 440 459 480 491 503 513 527 534 545 

S.D. 10.5 13.4 16.9 22.1 27.5 28.1 33.2 35.9 38.0 40.4 41.4 44.8 44.7 45.8 47.5 50.8

 N 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 257 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 4026 154 231 292 353 398 427 465 491 516 542 553 562 580 604 609 618 

 4027 142 205 263 306 344 368 396 415 437 449 457 465 468 472 482 498 

 4028 150 211 274 329 372 394 427 458 487 507 509 529 538 557 570 578 

 4029 170 236 303 364 414 451 495 518 544 569 581 592 606 622 632 642 

 4030 134 205 259 312 357 392 430 458 474 493 516 525 535 550 550 562 

 4031 141 206 256 299 330 347 366 380 400 418 429 441 444 457 466 480 

 4032 162 245 309 375 423 463 503 541 570 611 631 654 672 701 712 731 

 4033 130 207 255 312 358 389 414 439 452 454 472 480 489 506 506 511 

 4034 139 205 272 331 374 388 416 431 446 462 475 490 494 508 512 526 

 4035 156 226 275 321 354 381 407 433 455 452 413 448 462 481 509 502 

 4036 148 209 252 311 355 393 424 451 472 492 505 517 532 554 562 566 

 4037 137 205 257 307 346 368 396 417 431 454 459 472 480 493 513 524 

 4038 135 198 242 288 331 360 384 400 421 445 453 460 469 480 485 495 

 4039 152 217 274 304 349 373 393 412 436 453 467 475 488 502 517 528 

 4040 133 198 250 293 333 360 392 411 434 459 462 474 486 501 514 525 

 4041 142 213 257 306 334 354 380 410 435 441 458 470 478 486 501 504 

 4042 146 209 258 309 348 380 407 432 456 471 498 512 523 541 554 579 

 4043 161 224 276 324 358 394 412 434 447 465 463 475 481 496 502 501 

 4044 160 222 270 312 345 373 406 428 448 467 475 493 497 515 517 519 

 4045 140 210 268 331 378 403 424 483 480 500 514 520 535 552 558 562 

 4046 143 210 259 312 351 380 409 439 450 469 485 498 508 528 536 544 

 4047 154 220 269 326 375 412 429 459 482 502 528 519 537 550 564 574 

 4048 149 224 278 341 393 429 457 486 514 543 564 578 583 596 608 624 

 4049 136 206 255 305 338 368 399 417 425 447 462 356 442 475 494 505 

 4050 133 186 227 271 303 336 350 360 386 399 406 411 416 430 438 446 

 4051 132 204 258 310 343 378 411 428 449 466 478 488 502 512 518 528 

MEAN 145 213 266 317 358 387 415 440 459 478 489 496 509 526 536 545

S.D. 10.8 12.8 17.6 22.7 27.1 30.1 34.8 40.1 41.5 46.6 51.3 58.8 55.5 58.1 57.7 59.7 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 258 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 1026 574 592 

 1027 578 590 

 1028 536 553 

 1029 507 520 

 1030 566 572 

 1031 530 547 

 1032 595 619 

 1033 552 555 

 1034 495 509 

 1035 521 530 

 1036 566 588 

 1037 493 516 

 1038 563 579 

 1039 495 500 

 1040 645 654 

 1041 660 679 

 1042 671 682 

 1043 433 441 

 1044 603 619 

 1045 541 553 

 1046 579 586 

 1047 515 530 

 1048 600 614 

 1049 587 596 

 1050 576 595 

 1051 490 501 

MEAN 557 570

S.D. 55.9 57.0

 N 26 26
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 259 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 2026 498 500 

 2027 510 511 

 2028 554 564 

 2029 571 586 

 2030 609 625 

 2031 507 517 

 2032 482 490 

 2033 627 637 

 2034 604 610 

 2035 498 502 

 2036 481 489 

 2037 558 573 

 2038 530 544 

 2039 560 569 

 2040 570 579 

 2041 597 601 

 2042 537 544 

 2043 628 642 

 2044 650 659 

 2045 644 660 

 2046 548 559 

 2047 582 587 

 2048 485 489 

 2049 674 686 

 2050 592 603 

 2051 526 537 

MEAN 562 572

S.D. 56.0 58.0

 N 26 26
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 260 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 3026 558 573 

 3027 475 483 

 3028 607 623 

 3029 592 605 

 3030 612 622 

 3031 511 519 

 3032 505 509 

 3033 577 588 

 3034 578 591 

 3035 606 623 

 3036 487 501 

 3037 557 576 

 3038 550 565 

 3039 557 564 

 3040 633 649 

 3041 604 615 

 3042 538 546 

 3044 518 

 3045 465 472 

 3046 515 545 

 3047 540 543 

 3048 539 549 

 3049 530 542 

 3050 638 648 

 3051 625 642 

MEAN 557 570

S.D. 49.6 51.6 

 N 25 24
11-JAN-2010 16:53

Huntingdon Life Sciences 00-4205 PAGE 261 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

 4026 628 641 

 4027 502 509 

 4028 586 599 

 4029 642 657 

 4030 577 588 

 4031 490 503 

 4032 756 779 

 4033 522 528 

 4034 543 552 

 4035 508 526 

 4036 589 596 

 4037 533 544 

 4038 501 516 

 4039 538 550 

 4040 537 548 

 4041 508 524 

 4042 585 584 

 4043 517 519 

 4044 527 535 

 4045 566 571 

 4046 554 567 

 4047 593 601 

 4048 634 639 

 4049 514 527 

 4050 456 456 

 4051 540 543 

MEAN 555 566

S.D. 61.8 63.8

 N 26 26
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 262 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 1526 96 145 169 189 207 223 228 241 244 261 262 259 

 1527 105 148 174 198 213 231 241 254 260 269 275 277 

 1528 110 145 159 187 205 217 215 233 244 245 245 258 

 1529 117 154 174 194 214 219 232 245 256 254 263 263 

 1530 111 157 186 216 235 255 275 280 296 307 306 313 

 1531 115 154 170 188 199 223 235 244 247 247 257 262 

 1532 117 165 188 207 242 253 260 281 295 289 288 298 

 1533 112 161 183 216 238 256 262 282 287 295 292 295 

 1534 109 160 177 214 233 256 264 272 278 286 289 292 

 1535 94 145 167 202 219 239 252 261 283 285 295 306 

 1536 99 156 187 218 235 256 268 279 276 289 296 306 

 1537 103 162 192 213 238 243 259 275 277 275 282 288 

 1538 120 177 205 238 262 279 298 302 314 322 328 324 

 1539 119 176 203 230 252 273 285 294 296 302 312 312 

 1540 122 161 180 195 216 230 237 242 254 261 256 263 264 268 272 271 

 1541 103 152 180 204 229 246 247 267 279 287 284 297 

 1542 104 152 175 203 228 241 242 256 265 272 275 277 

 1543 113 151 187 211 229 238 255 272 279 274 293 298 

 1544 106 156 182 216 233 253 267 278 287 293 302 304 

 1545 114 151 169 188 207 218 223 238 245 248 249 258 

 1546 100 142 168 190 211 225 219 238 248 249 260 264 

 1547 113 158 180 210 230 245 245 261 266 273 269 283 

 1548 102 143 163 184 203 218 218 227 238 243 248 258 

 1549 112 147 171 197 214 235 237 255 256 260 270 266 297 

 1550 109 152 174 199 220 229 236 247 254 262 265 271 

 1551 105 151 175 199 218 229 241 187 249 258 265 268 

MEAN 109 155 178 204 224 240 248 258 268 273 278 283 264 268 272 284 

S.D. 7.5 8.9 11.2 13.7 15.6 17.0 21.0 24.5 20.3 21.2 21.3 20.6 0.0 0.0 0.0 18.2

 N 26 26 26 26 26 26 26 26 26 26 26 26 1 1 1 2
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 263 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 2526 100 140 159 187 207 206 229 232 243 253 239 253 

 2527 108 150 175 198 220 232 250 259 265 273 280 277 

 2528 114 159 184 209 224 242 250 269 273 284 277 291 

 2529 117 160 183 202 228 238 253 257 262 272 273 271 

 2530 104 147 176 200 218 241 249 253 270 278 276 283 

 2531 119 158 187 211 232 238 257 269 278 279 288 292 

 2532 121 179 201 222 228 253 254 270 283 284 288 298 

 2533 119 167 189 223 252 289 284 301 304 304 319 325 

 2534 114 159 189 212 229 255 273 273 285 288 285 300 

 2535 111 158 180 202 220 234 246 259 262 266 271 272 

 2536 116 146 174 194 215 232 243 253 253 264 260 263 

 2537 110 169 186 219 244 252 274 281 292 292 299 302 

 2538 112 166 202 236 259 280 304 321 319 338 344 339 

 2539 106 156 182 210 232 250 260 261 272 279 282 285 

 2540 103 146 175 193 206 226 239 246 242 256 264 259 

 2541 113 154 177 141 210 235 254 264 260 273 278 275 

 2542 109 158 184 208 227 242 248 261 266 274 271 288 

 2543 112 149 172 194 213 215 228 241 248 246 260 267 279 307 334 

 2544 93 140 168 192 215 232 243 257 265 273 268 278 278 278 276 282 

 2545 97 141 171 196 221 229 255 274 276 278 298 295 

 2546 96 149 174 196 221 229 239 258 264 270 282 284 

 2547 107 146 165 188 203 216 220 235 241 245 245 254 

 2548 103 139 165 184 210 223 233 239 253 260 263 268 

 2549 121 169 185 216 229 249 260 272 275 281 288 289 289 298 300 303 

 2550 100 148 183 181 201 208 210 226 230 237 237 248 

 2551 105 150 158 216 230 244 263 277 275 295 297 291 

MEAN 109 154 179 201 223 238 251 262 268 275 278 283 282 294 303 292

S.D. 7.9 10.3 11.0 18.2 14.0 18.9 19.8 20.5 19.7 20.4 23.1 21.0 6.6 14.9 29.0 14.5 

 N 26 26 26 26 26 26 26 26 26 26 26 26 3 3 3 2
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 264 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 3526 109 148 172 204 227 233 256 266 271 280 282 286 280 294 279 292 

 3527 105 138 169 198 211 238 257 267 269 286 292 281 

 3528 122 162 198 230 260 274 303 318 324 324 340 334 

 3529 92 139 162 186 204 219 227 242 245 251 259 260 

 3530 112 166 190 209 236 252 263 283 291 298 300 303 312 308 306 325 

 3531 104 146 169 198 219 234 249 261 269 273 274 284 

 3532 99 138 163 189 205 211 230 246 249 255 261 264 

 3533 95 141 157 190 207 223 242 252 253 268 274 265 

 3534 117 157 172 206 225 236 241 261 267 263 271 277 312 

 3535 105 138 163 176 205 223 231 237 247 253 253 264 

 3536 107 139 164 186 195 214 233 241 247 253 252 264 

 3537 119 154 164 191 206 216 221 234 238 245 245 258 

 3538 114 160 184 218 235 253 260 282 281 296 307 301 

 3539 107 155 181 208 233 244 264 279 278 300 297 296 

 3540 113 155 181 197 222 240 255 263 276 286 289 290 

 3541 120 160 186 209 231 249 262 276 282 292 294 308 

 3542 100 138 165 179 204 217 221 243 250 267 270 274 

 3543 102 154 173 194 212 224 237 244 245 257 260 259 

 3544 113 148 173 197 214 209 232 242 246 250 242 255 

 3545 116 167 201 224 242 245 251 267 272 287 277 294 

 3546 116 161 190 211 234 249 268 279 286 290 301 297 

 3547 109 141 172 188 211 214 228 237 241 244 249 248 

 3548 101 154 186 212 234 251 262 264 279 284 284 283 

 3549 111 148 177 197 214 226 232 251 246 253 262 260 

 3550 112 147 170 195 220 225 246 256 259 265 270 273 

 3551 103 152 178 196 224 230 248 253 256 273 282 275 

MEAN 109 150 175 199 220 233 247 259 264 273 276 279 296 301 293 310 

S.D. 7.8 9.3 11.5 13.2 14.8 16.2 18.4 19.1 19.9 20.3 22.5 20.1 22.6 10.0 19.4 16.7 

 N 26 26 26 26 26 26 26 26 26 26 26 26 2 2 2 3
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 265 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

 4526 109 145 165 192 205 224 230 248 257 259 260 268 

 4527 107 146 169 196 208 225 244 254 255 262 266 266 

 4528 117 165 182 212 229 249 255 279 287 294 286 304 

 4529 110 161 188 209 233 254 258 280 291 295 306 306 

 4530 111 153 175 202 217 239 253 265 262 285 286 286 303 325 368 

 4531 116 159 182 200 220 232 244 248 273 266 272 278 

 4532 118 151 172 192 209 222 237 244 246 248 256 260 

 4533 104 146 168 184 200 210 230 234 243 243 251 253 

 4534 105 150 171 187 200 211 229 235 237 251 254 252 

 4535 120 169 204 228 259 260 278 292 297 295 309 315 

 4536 121 176 192 225 248 264 272 286 297 302 300 310 

 4537 113 153 186 214 237 243 261 274 281 279 287 291 

 4538 94 144 174 201 219 236 247 249 264 276 278 284 

 4539 113 141 156 176 185 195 208 224 225 228 233 241 

 4540 105 158 188 225 232 275 289 295 304 317 336 341 

 4541 114 160 176 199 204 221 232 243 240 253 256 259 

 4542 112 146 158 185 195 206 215 230 235 239 236 247 

 4543 99 141 163 175 201 219 225 231 245 258 258 263 263 271 287 258 

 4544 103 147 175 192 218 227 245 258 267 277 287 287 290 306 333 317 

 4545 102 148 169 191 208 224 237 249 251 264 277 277 

 4546 119 164 195 220 236 243 262 278 290 288 303 306 

 4547 103 155 181 213 234 255 269 286 299 311 312 332 

 4548 93 130 155 174 191 201 210 212 224 231 233 238 

 4549 113 154 171 184 203 216 228 231 249 259 256 262 

 4550 100 141 157 179 193 198 206 220 224 225 235 237 

 4551 107 150 168 194 205 219 230 247 247 248 256 261 263 261 270 279 

MEAN 109 152 175 198 215 229 242 253 261 267 273 278 280 291 314 285 

S.D. 7.6 10.1 12.5 16.2 18.8 21.1 21.8 23.8 25.3 25.7 27.3 28.7 20.2 29.6 44.5 29.9

 N 26 26 26 26 26 26 26 26 26 26 26 26 4 4 4 3
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 266 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

 1540 282 

 1549 294 310 311 

MEAN 288 310 311

S.D. 8.6 0.0 0.0

 N 2 1 1
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 267 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

 2544 294 

 2549 304 

MEAN 299 

S.D. 7.1 

 N 2 
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 268 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

 3526 295 

 3530 333 

 3534 306 307 312 

MEAN 311 307 312

S.D. 19.8 0.0 0.0

 N 3 1 1
11-JAN-2010 16:55

Huntingdon Life Sciences 00-4205 PAGE 269 

 

 APPENDIX G P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHTS (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

 4543 271 

 4544 324 

 4551 275 

MEAN 290 

S.D. 29.5 

 N 3 
Huntingdon Life Sciences 00-4205 Page 270

211-DIPE-1G Final Report

Individual Body Weight Gain

(Premating/Mating/Postmating)

Preface Appendix H

No data is presented for the following animals at the stated intervals for the 

following reasons:

Animal No. Intervals Reason

3043 21-28 

through 

98-105

Sacrificed for Humane Reasons on Day 23

3044 105-112

and

0-112

Found Dead on Day 110

Note: Body weight gains for females are presented for the premating period only 

(Days –7 to 0 through 63-69 and 0-69).
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 271 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 67 58 54 44 33 30 31 25 21 10 11 19 8 5 17 0 

 1027 66 51 57 39 44 33 27 16 16 22 1 15 15 5 11 10 

 1028 64 50 44 45 38 26 24 26 16 15 6 11 10 6 8 9 

 1029 60 55 46 35 35 27 26 15 20 18 2 3 12 3 10 7 

 1030 77 57 53 42 30 25 28 20 12 7 15 4 19 1 19 9 

 1031 63 57 56 43 20 35 15 15 24 15 13 -1 -8 14 17 7 

 1032 76 55 53 54 9 35 31 18 18 8 23 -3 19 5 16 12 

 1033 82 48 46 32 40 35 22 21 24 -19 -8 20 28 11 9 18 

 1034 56 49 45 38 27 24 23 18 18 18 9 16 7 2 16 -3 

 1035 66 44 44 43 24 30 25 17 11 14 11 8 21 1 11 14 

 1036 77 61 61 44 21 24 33 19 13 13 7 10 9 12 25 -8 

 1037 67 52 48 36 25 26 24 15 7 19 14 2 13 2 12 -1 

 1038 70 51 52 34 36 21 27 15 27 8 10 17 14 10 11 8 

 1039 65 44 50 37 30 29 32 5 23 -1 8 11 9 13 6 2 

 1040 77 64 60 50 40 38 34 21 32 8 25 8 14 8 17 13 

 1041 73 60 70 52 46 42 24 23 6 22 30 16 10 7 19 11 

 1042 70 58 63 50 42 33 32 17 34 16 17 13 27 18 18 11 

 1043 62 47 42 27 9 17 17 20 9 11 13 4 2 4 14 5 

 1044 83 59 59 47 35 30 28 19 9 25 15 10 9 15 14 12 

 1045 63 49 44 42 21 26 22 16 21 9 20 6 18 6 9 8 

 1046 78 62 45 41 11 30 34 21 13 14 21 -6 22 9 22 10 

 1047 57 46 47 49 26 32 15 26 14 10 16 -3 11 5 6 18 

 1048 57 61 56 26 32 40 35 21 23 31 -1 17 2 16 19 6 

 1049 68 52 57 35 35 28 28 15 23 26 11 14 15 12 13 7 

 1050 73 56 54 45 22 24 -83a 101 29 15 17 22 20 2 13 4 

 1051 62 53 37 38 37 20 15 17 12 16 6 38 -18 2 13 6 

MEAN 68 54 52 41 30 29 22 22 18 13 12 10 12 7 14 8

S.D. 7.8 5.7 7.6 7.2 10.5 6.1 22.2 16.8 7.5 9.5 8.3 9.5 9.9 5.1 4.8 5.9 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

a

THIS ANIMAL’S WATER VALVE WAS NOTED AS NOT WORKING PROPERLY ON DAY 42. THE VALVE WAS REPAIRED IMMEDIATELY.
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 272 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 66 44 44 30 25 30 22 20 12 19 12 7 22 -9 10 11 

 2027 67 45 42 40 28 28 24 14 16 9 13 11 7 8 6 12 

 2028 68 46 52 47 30 34 22 18 17 15 19 2 18 11 6 14 

 2029 69 55 55 51 34 36 25 19 15 20 8 4 18 1 3 11 

 2030 67 56 55 55 46 39 27 27 27 15 16 6 19 8 -1 9 

 2031 65 54 48 38 29 27 23 10 14 18 4 5 17 2 5 9 

 2032 60 53 48 41 26 24 24 13 6 11 13 -1 17 6 6 3 

 2033 81 65 60 51 29 34 29 21 21 21 22 6 23 13 7 15 

 2034 68 57 54 41 37 40 21 18 23 20 12 15 15 15 9 7 

 2035 65 52 44 32 24 11 26 8 21 15 6 8 19 5 10 8 

 2036 73 46 47 30 25 18 23 13 12 9 6 4 15 9 8 10 

 2037 65 49 54 40 27 34 33 23 13 18 13 3 18 4 6 5 

 2038 65 53 43 39 31 28 22 12 18 14 15 11 11 5 20 0 

 2039 61 49 51 41 28 35 16 23 12 22 15 17 9 13 9 7 

 2040 68 53 48 47 32 32 19 27 15 14 15 14 11 3 24 0 

 2041 67 58 50 45 29 31 31 17 22 11 16 9 12 15 15 5 

 2042 62 49 44 46 39 29 31 11 14 12 11 9 9 8 13 1 

 2043 62 55 52 49 34 39 30 30 16 26 5 14 21 3 17 14 

 2044 74 61 60 49 27 35 30 29 18 28 7 12 22 16 13 10 

 2045 72 64 63 42 38 42 34 23 19 17 16 7 6 15 20 2 

 2046 69 55 41 34 34 29 22 18 18 15 15 5 11 16 16 9 

 2047 67 54 54 44 36 30 28 24 18 24 5 19 18 2 15 14 

 2048 64 46 43 35 20 33 17 13 16 16 6 13 6 10 16 0 

 2049 71 62 58 46 47 43 40 32 15 30 15 11 16 6 18 9 

 2050 67 54 53 48 33 33 24 20 18 7 19 11 16 21 1 18 

 2051 55 39 42 44 29 34 19 24 15 14 20 17 11 9 8 9 

MEAN 67 53 50 42 31 32 26 19 16 17 12 9 15 8 11 8

S.D. 5.0 6.4 6.3 6.7 6.3 7.0 5.7 6.5 4.3 5.8 5.1 4.9 5.1 6.4 6.3 5.0 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 273 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 70 53 52 44 44 28 30 16 24 13 9 5 11 15 5 6 

 3027 60 46 47 32 32 25 18 16 17 5 8 7 16 2 2 10 

 3028 63 58 55 43 40 38 25 25 24 14 18 4 18 3 11 14 

 3029 70 54 51 38 30 25 27 19 29 20 11 12 23 5 22 14 

 3030 75 64 62 51 42 38 24 18 32 4 24 1 17 2 13 3 

 3031 70 56 49 30 27 35 22 6 18 18 11 9 13 -7 11 9 

 3032 56 50 43 37 27 22 20 24 18 11 13 8 6 11 -5 23 

 3033 64 64 58 43 32 25 20 15 19 14 10 8 25 19 16 12 

 3034 67 55 58 37 26 38 27 11 26 11 15 12 13 16 20 -2 

 3035 65 56 58 43 36 38 22 16 21 7 10 11 24 17 12 18 

 3036 62 50 52 35 28 24 16 15 20 2 0 17 12 2 8 14 

 3037 66 57 59 41 24 26 25 12 21 14 4 18 11 4 11 19 

 3038 60 59 51 39 25 27 24 23 21 4 18 4 6 14 9 27 

 3039 84 54 49 38 21 33 22 28 24 0 16 9 8 12 16 10 

 3040 79 61 60 47 36 38 28 23 17 18 10 17 20 12 19 11 

 3041 85 65 57 45 26 38 21 28 18 14 12 6 13 13 15 2 

 3042 72 60 55 37 24 25 11 22 14 14 6 3 10 1 14 14 

 3043 75 67 71 -73 

 3044 58 56 35 33 24 24 16 24 25 15 13 7 19 2 9 5 

 3045 58 54 39 31 15 19 16 24 15 9 6 10 10 10 4 6 

 3046 68 51 41 29 25 22 28 5 12 15 8 9 10 5 14 17 

 3047 61 51 55 37 25 39 28 16 15 9 17 11 18 6 5 9 

 3048 59 52 46 47 27 25 23 20 26 9 9 10 20 9 12 13 

 3049 63 49 37 33 29 26 16 9 23 16 13 11 13 4 6 11 

 3050 70 64 63 46 34 30 33 24 32 12 22 12 6 5 14 8 

 3051 70 61 52 40 35 46 24 28 21 13 16 14 13 10 5 15 

MEAN 67 56 52 35 29 30 23 19 21 11 12 9 14 8 11 12 

S.D. 7.7 5.6 8.5 22.7 6.8 7.3 5.2 6.8 5.3 5.3 5.4 4.3 5.5 6.2 6.1 6.5

 N 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 274 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 69-77 77-84 84-91 91-98 98-105 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 77 62 61 45 29 38 26 24 27 11 8 19 24 5 10 9 

 4027 64 57 44 38 24 28 19 22 12 9 8 3 4 9 16 4 

 4028 61 63 56 42 22 33 31 29 20 2 20 9 19 13 7 8 

 4029 67 66 61 51 37 43 23 26 24 12 12 13 17 10 10 1 

 4030 71 55 53 45 35 37 29 16 19 22 9 10 15 0 12 16 

 4031 65 50 42 32 17 19 14 20 18 11 12 3 12 9 14 10 

 4032 83 64 66 48 40 41 37 29 41 20 22 18 30 11 19 25 

 4033 77 48 58 46 31 25 25 13 3 17 9 9 17 0 5 11 

 4034 65 67 59 42 15 28 15 15 17 13 14 5 14 3 15 16 

 4035 70 49 46 33 27 27 26 21 -3 -38 35 14 19 28 -7 6 

 4036 62 43 58 44 38 31 26 21 20 13 12 15 22 8 4 23 

 4037 68 52 50 39 22 29 21 15 23 5 12 9 13 20 11 9 

 4038 63 44 46 43 29 25 16 21 23 8 7 9 11 5 10 6 

 4039 65 57 30 45 25 20 19 24 18 14 8 13 15 14 12 10 

 4040 65 52 43 40 27 33 18 23 26 3 12 13 15 13 11 12 

 4041 71 44 49 28 21 26 29 25 6 17 12 8 8 15 3 4 

 4042 63 49 51 39 32 27 25 24 15 27 15 10 19 12 25 6 

 4043 63 53 47 35 36 18 22 14 17 -2 12 6 15 6 0 15 

 4044 62 48 42 34 28 32 22 20 19 8 19 4 18 2 2 8 

 4045 70 58 63 47 26 21 59 -3 21 14 6 14 17 6 4 4 

 4046 66 49 53 39 29 30 30 12 19 15 14 10 20 7 8 10 

 4047 66 49 57 49 37 18 30 23 19 27 -9 18 13 14 10 19 

 4048 75 55 63 51 36 29 28 28 30 21 14 5 13 12 16 10 

 4049 70 48 50 34 29 32 17 8 22 15 -106 86 33 20 10 9 

 4050 54 40 44 32 33 13 11 26 13 8 5 5 13 8 9 9 

 4051 72 53 53 33 35 33 18 21 17 13 9 14 10 7 10 11 

MEAN 67 53 52 40 29 28 24 20 19 11 7 13 16 10 9 10

S.D. 6.2 7.2 8.4 6.6 6.6 7.4 9.3 7.3 8.5 12.3 24.3 15.6 6.1 6.5 6.4 5.8 

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 275 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 18 316 383 

 1027 13 306 374 

 1028 17 288 349 

 1029 13 279 326 

 1030 5 287 344 

 1031 17 292 337 

 1032 24 304 377 

 1033 3 242 330 

 1034 14 268 320 

 1035 9 262 326 

 1036 22 297 368 

 1037 23 267 316 

 1038 16 279 355 

 1039 5 258 303 

 1040 10 372 442 

 1041 20 376 458 

 1042 11 362 459 

 1043 8 213 249 

 1044 16 324 401 

 1045 12 269 327 

 1046 7 291 354 

 1047 15 281 332 

 1048 14 324 396 

 1049 8 311 380 

 1050 19 281 361 

 1051 10 250 302 

MEAN 13 292 356

S.D. 5.7 38.2 48.5

 N 26 26 26
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 276 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 2 256 300 

 2027 1 259 303 

 2028 10 299 361 

 2029 16 318 370 

 2030 16 362 419 

 2031 10 265 313 

 2032 8 259 297 

 2033 10 353 426 

 2034 7 322 390 

 2035 5 239 294 

 2036 8 227 281 

 2037 16 303 354 

 2038 14 276 336 

 2039 9 292 356 

 2040 9 300 361 

 2041 4 311 371 

 2042 8 286 334 

 2043 14 337 419 

 2044 10 344 426 

 2045 16 356 422 

 2046 11 280 347 

 2047 5 315 387 

 2048 4 245 294 

 2049 12 388 460 

 2050 11 310 387 

 2051 12 280 346 

MEAN 9 299 360

S.D. 4.3 41.2 49.7

 N 26 26 26
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 277 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 15 313 370 

 3027 8 245 290 

 3028 16 339 406 

 3029 13 302 390 

 3030 10 360 406 

 3031 8 272 315 

 3032 4 264 310 

 3033 11 299 389 

 3034 13 303 376 

 3035 17 306 405 

 3036 14 242 309 

 3037 19 282 363 

 3038 15 291 365 

 3039 8 283 345 

 3040 16 338 433 

 3041 11 324 382 

 3042 8 267 317 

 3044 264 

 3045 8 227 274 

 3046 30 236 321 

 3047 3 291 343 

 3048 11 284 358 

 3049 12 254 310 

 3050 10 360 416 

 3051 17 336 410 

MEAN 12 291 358

S.D. 5.6 37.6 44.5

 N 24 25 24
11-JAN-2010 16:57

Huntingdon Life Sciences 00-4205 PAGE 278 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 105-112 0-69 0-112 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 14 331 410 

 4027 7 260 304 

 4028 13 318 388 

 4029 14 356 420 

 4030 11 320 384 

 4031 13 235 297 

 4032 24 408 534 

 4033 7 273 321 

 4034 10 285 348 

 4035 18 222 301 

 4036 7 308 387 

 4037 11 266 339 

 4038 15 262 318 

 4039 12 258 333 

 4040 12 276 351 

 4041 16 257 311 

 4042 -1 303 375 

 4043 2 252 295 

 4044 9 272 314 

 4045 5 310 361 

 4046 13 289 358 

 4047 8 299 381 

 4048 5 354 416 

 4049 13 150 321 

 4050 1 225 270 

 4051 4 283 339 

MEAN 10 284 353

S.D. 5.6 50.6 54.8

 N 26 26 26
11-JAN-2010 17:00

Huntingdon Life Sciences 00-4205 PAGE 279 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 49 24 20 18 16 5 13 3 17 1 -3 114 

 1527 42 26 24 15 18 11 13 6 9 6 2 129 

 1528 35 14 28 18 12 -2 19 11 1 0 13 112 

 1529 37 20 20 19 6 13 13 11 -3 9 0 109 

 1530 47 29 30 19 20 20 5 15 12 -2 7 156 

 1531 40 15 18 11 24 12 8 4 -1 11 5 108 

 1532 48 24 19 35 11 7 21 14 -6 -1 10 134 

 1533 49 22 33 22 18 6 19 6 8 -3 4 134 

 1534 51 17 37 19 23 9 8 6 8 3 3 132 

 1535 51 22 35 18 20 13 9 22 2 10 12 161 

 1536 57 31 31 17 21 13 11 -4 13 7 10 150 

 1537 59 31 20 26 5 16 16 2 -2 7 6 126 

 1538 57 28 33 25 17 19 4 12 8 5 -4 147 

 1539 57 28 27 22 21 13 9 2 6 10 1 137 

 1540 39 19 14 22 14 6 6 11 8 -5 7 102 

 1541 49 29 24 25 17 1 20 12 8 -3 12 145 

 1542 48 23 28 25 14 1 13 9 7 3 2 125 

 1543 39 35 25 17 9 17 17 8 -6 20 5 147 

 1544 49 27 33 17 21 14 11 9 6 10 2 149 

 1545 37 19 19 19 11 5 15 7 3 2 9 107 

 1546 42 26 22 21 14 -5 19 10 1 11 5 123 

 1547 45 22 29 21 15 0 15 5 7 -4 14 125 

 1548 42 20 21 19 15 0 9 11 5 6 10 115 

 1549 35 25 25 18 20 3 18 1 4 10 -4 119 

 1550 43 22 25 21 10 7 11 8 8 3 6 119 

 1551 46 24 25 19 11 12 -54a 62 9 8 3 117 

MEAN 46 24 26 20 15 8 10 10 5 5 5 129

S.D. 7.1 5.1 5.8 4.4 5.1 6.8 14.0 11.8 5.5 5.8 5.1 16.4

 N 26 26 26 26 26 26 26 26 26 26 26 26

a

THIS ANIMAL’S WATER VALVE WAS NOTED AS NOT WORKING PROPERLY ON DAY 42. THE VALVE WAS REPAIRED IMMEDIATELY.
11-JAN-2010 17:00

Huntingdon Life Sciences 00-4205 PAGE 280 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 41 18 29 20 -1 23 3 11 10 -14 14 113 

 2527 42 25 24 21 12 18 9 6 7 7 -2 128 

 2528 46 25 24 15 18 8 19 4 10 -7 14 132 

 2529 43 23 19 26 10 16 4 6 10 1 -2 111 

 2530 43 30 24 18 23 8 4 17 8 -3 7 136 

 2531 39 29 25 21 6 19 12 8 1 9 4 134 

 2532 58 22 21 7 25 1 16 14 1 5 9 119 

 2533 48 22 34 28 37 -5 17 2 0 15 6 158 

 2534 45 30 23 17 26 18 0 12 3 -2 15 141 

 2535 47 23 22 19 14 12 12 4 4 5 0 114 

 2536 30 28 20 21 18 11 10 0 11 -4 3 117 

 2537 59 18 33 25 8 21 8 11 -1 7 2 133 

 2538 53 37 34 22 21 24 17 -2 19 7 -5 174 

 2539 50 26 28 22 18 10 1 12 7 3 4 129 

 2540 43 29 18 13 21 12 8 -5 15 8 -5 113 

 2541 41 23 -36 68 25 19 10 -3 13 5 -3 121 

 2542 49 26 24 19 15 6 13 5 8 -3 17 130 

 2543 37 23 22 19 2 13 13 7 -2 14 7 118 

 2544 48 28 24 23 17 11 14 8 9 -5 10 138 

 2545 44 30 25 25 9 25 19 2 2 20 -3 154 

 2546 52 26 22 25 8 9 19 6 6 12 2 135 

 2547 39 20 22 15 13 4 15 6 4 -1 9 108 

 2548 36 26 19 26 13 10 6 14 7 3 6 130 

 2549 48 16 30 13 20 11 12 3 7 7 0 120 

 2550 47 36 -3 20 7 2 17 4 6 0 11 100 

 2551 45 9 57 14 14 19 14 -2 20 2 -6 142 

MEAN 45 25 22 22 15 13 11 6 7 4 4 129

S.D. 6.5 5.9 15.2 10.8 8.3 7.6 5.7 5.6 5.6 7.4 6.7 16.6

 N 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 17:00

Huntingdon Life Sciences 00-4205 PAGE 281 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 39 25 32 22 7 22 10 5 9 2 4 139 

 3527 34 31 29 13 27 19 10 2 17 6 -11 143 

 3528 41 36 32 30 14 29 15 6 -1 17 -6 172 

 3529 46 24 23 19 15 8 15 2 7 7 2 122 

 3530 53 24 20 27 16 10 20 8 7 2 3 138 

 3531 43 23 29 22 14 15 13 8 5 1 11 138 

 3532 39 25 26 17 5 19 16 4 6 6 3 126 

 3533 47 16 33 17 16 19 10 1 15 6 -8 124 

 3534 41 14 34 19 11 5 20 7 -4 7 7 120 

 3535 32 25 13 30 18 8 6 11 6 -1 12 127 

 3536 32 24 22 10 19 19 9 6 6 -2 12 125 

 3537 35 10 27 15 10 5 13 3 8 0 13 104 

 3538 46 24 33 17 18 7 22 -2 15 12 -6 141 

 3539 48 27 26 26 11 20 15 -1 22 -3 -1 141 

 3540 42 27 16 25 18 16 8 13 10 3 1 135 

 3541 40 25 24 22 18 14 13 6 10 2 14 147 

 3542 38 27 14 26 13 4 22 7 17 3 4 136 

 3543 52 20 21 18 12 13 7 1 12 3 -1 105 

 3544 34 26 24 17 -5 23 10 4 4 -8 13 107 

 3545 50 34 23 18 3 6 16 5 15 -10 17 127 

 3546 45 29 21 23 15 19 12 6 5 10 -4 136 

 3547 32 31 16 23 3 14 9 3 4 5 -1 107 

 3548 53 32 26 21 17 12 2 15 5 0 -1 129 

 3549 37 29 20 16 13 6 19 -4 7 9 -3 111 

 3550 35 23 25 25 5 21 10 4 6 4 3 126 

 3551 49 26 17 29 6 18 5 3 17 9 -7 123 

MEAN 42 25 24 21 12 14 13 5 9 3 3 129

S.D. 6.8 5.8 6.1 5.2 6.7 6.8 5.2 4.3 6.0 5.8 7.6 15.2

 N 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 17:00

Huntingdon Life Sciences 00-4205 PAGE 282 

 

 APPENDIX H P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL BODY WEIGHT GAIN (GRAMS) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# -7-0 0-7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-69 0-69 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 36 20 26 13 20 6 18 9 2 1 8 123 

 4527 39 23 26 12 17 19 10 1 7 4 0 120 

 4528 48 17 31 17 20 6 24 9 7 -8 18 139 

 4529 51 27 21 24 21 4 22 12 4 11 -1 145 

 4530 42 22 27 15 22 14 12 -3 23 1 0 133 

 4531 43 23 18 20 12 13 4 24 -7 7 6 120 

 4532 33 22 20 17 13 15 6 2 2 9 3 109 

 4533 42 22 17 16 9 21 4 9 0 8 2 107 

 4534 45 21 16 13 10 18 6 1 14 4 -2 103 

 4535 50 34 25 30 1 18 14 4 -2 13 7 146 

 4536 55 16 33 24 15 8 14 11 5 -2 10 135 

 4537 40 33 29 23 6 18 13 7 -2 8 3 138 

 4538 50 30 27 18 17 11 2 15 13 2 6 140 

 4539 28 15 20 9 10 13 16 1 3 5 7 100 

 4540 53 30 38 7 42 14 7 9 13 19 5 183 

 4541 46 16 23 5 17 11 11 -3 14 3 3 99 

 4542 34 12 27 10 12 8 16 5 4 -4 11 101 

 4543 42 22 12 26 18 6 6 14 12 0 6 122 

 4544 45 28 17 26 9 18 13 9 10 9 1 140 

 4545 47 21 22 17 16 13 13 2 13 13 0 129 

 4546 45 31 25 16 7 19 16 12 -2 15 4 142 

 4547 51 27 32 21 21 14 17 13 13 1 20 178 

 4548 37 26 19 17 10 9 2 12 7 2 5 108 

 4549 41 17 13 19 14 11 3 18 9 -2 5 108 

 4550 41 16 22 14 5 8 14 3 2 10 1 95 

 4551 43 18 26 11 14 11 17 0 1 8 5 111 

MEAN 43 23 24 17 14 12 12 8 6 5 5 126

S.D. 6.5 6.0 6.2 6.2 7.9 4.8 6.1 6.7 6.8 6.3 5.3 22.7

 N 26 26 26 26 26 26 26 26 26 26 26 26
Huntingdon Life Sciences 00-4205 Page 283

211-DIPE-1G Final Report

Individual Gestation Body Weights

Preface Appendix I

No data is presented for the following animal at the stated intervals for the 

following reasons:

Animal No.

Gestation

Days Reason

2536 4 to 20 Sacrificed for Humane Reasons on Gestation 

Day 1 
11-JAN-2010 17:01

Huntingdon Life Sciences 00-4205 PAGE 284 

 

 APPENDIX I P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 1526 263 279 284 313 376 

 1527 266 293 301 324 384 

 1528 250 264 274 295 330 

 1529 264 285 295 325 378 

 1530 303 330 335 367 395 

 1531 263 285 291 325 377 

 1532 294 314 314 340 396 

 1533 295 314 320 353 424 

 1534x NP 283 307 315 343 327 

 1535 300 320 324 345 401 

 1536 297 325 339 373 432 

 1537 286 312 327 358 430 

 1538x NP 330 348 349 345 344 

 1539 309 332 345 372 435 

 1540xNNMD 

 1541 295 315 329 357 419 

 1542 284 298 305 329 390 

 1543 289 315 332 357 423 

 1544 310 334 338 367 423 

 1545 252 271 280 303 372 

 1546 253 289 292 324 390 

 1547 276 296 305 328 375 

 1548x NP 252 269 277 275 275 

 1549 TLL 276 293 302 322 387 

 1550 271 286 296 312 377 

 1551 256 277 282 299 313 

MEAN 280 301 309 336 392

S.D. 19.3 20.7 21.6 24.4 31.2

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:01

Huntingdon Life Sciences 00-4205 PAGE 285 

 

 APPENDIX I P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 2526 257 283 296 329 405 

 2527 273 290 299 324 363 

 2528 291 315 325 342 413 

 2529 270 284 293 321 381 

 2530 283 304 308 338 400 

 2531 289 305 311 337 397 

 2532 291 312 320 346 404 

 2533 319 351 351 384 449 

 2534 294 311 320 350 409 

 2535 273 289 298 328 383 

 2536 HS 215 

 2537 283 310 319 347 372 

 2538 336 361 376 402 458 

 2539 285 302 310 335 399 

 2540 264 277 286 309 359 

 2541 282 303 313 341 409 

 2542 285 306 310 342 406 

 2543xPNMD 

 2544xNNMD 

 2545 296 326 337 367 426 

 2546 280 304 320 350 404 

 2547 251 267 271 298 353 

 2548 271 285 294 318 376 

 2549xNNMD 

 2550 245 270 282 305 326 

 2551 307 329 341 365 423 

MEAN 280 304 313 340 396

S.D. 24.9 23.5 23.9 24.7 30.7

 N 23 22 22 22 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN 
11-JAN-2010 17:01

Huntingdon Life Sciences 00-4205 PAGE 286 

 

 APPENDIX I P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 3526xNNMD 

 3527 279 315 328 358 428 

 3528 335 367 381 415 471 

 3529 252 277 287 314 379 

 3530xNNMD 

 3531 282 300 309 327 369 

 3532 266 284 292 320 373 

 3533 268 294 303 337 393 

 3534 TLL 278 298 306 326 332 

 3535 259 282 288 315 383 

 3536 262 278 289 312 370 

 3537 258 287 304 334 358 

 3538 314 337 349 374 424 

 3539 298 331 344 370 423 

 3540 290 316 327 353 377 

 3541 312 327 338 373 436 

 3542 280 305 320 355 380 

 3543 263 279 287 311 381 

 3544 247 271 279 304 352 

 3545 293 323 338 358 414 

 3546 298 317 331 355 429 

 3547 241 256 263 289 340 

 3548 284 305 318 345 417 

 3549 265 280 291 316 389 

 3550 277 297 303 328 398 

 3551 283 307 313 342 419 

MEAN 278 301 312 339 393

S.D. 22.4 25.0 26.8 28.2 33.6

 N 24 24 24 24 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:01

Huntingdon Life Sciences 00-4205 PAGE 287 

 

 APPENDIX I P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

 4526 262 289 293 319 365 

 4527 273 284 319 310 371 

 4528 282 317 320 347 417 

 4529 308 338 347 387 462 

 4530xPNMD 

 4531 270 292 307 319 392 

 4532 253 280 286 315 372 

 4533 252 272 279 307 345 

 4534 257 289 301 330 396 

 4535 307 326 342 373 433 

 4536 310 325 335 361 428 

 4537 290 321 337 370 427 

 4538 HS 276 307 317 354 415 

 4539 244 258 269 296 345 

 4540 326 360 372 391 455 

 4541 262 284 290 320 387 

 4542 239 257 266 291 320 

 4543xNNMD 

 4544xNNMD 

 4545 271 290 298 330 401 

 4546 310 333 347 386 442 

 4547 328 357 366 401 470 

 4548 234 257 261 293 355 

 4549 265 280 291 319 373 

 4550 236 252 260 291 359 

 4551x NP 

MEAN 275 299 309 337 397

S.D. 28.8 32.1 33.7 35.5 41.6

 N 22 22 22 22 22

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 

 x=EXCLUDED FROM MEAN 
Huntingdon Life Sciences 00-4205 Page 288

211-DIPE-1G Final Report

Individual Gestation Body Weight Gain

Preface Appendix J

No data is presented for the following animal at the stated intervals for the 

following reasons:

Animal No.

Gestation

Days Reason

2536 0-4 through 

14-20 and 

0-20

Sacrificed for Humane Reasons on Gestation 

Day 1 
11-JAN-2010 17:03

Huntingdon Life Sciences 00-4205 PAGE 289 

 

 APPENDIX J P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 1526 16 5 29 63 113 

 1527 28 7 23 60 118 

 1528 13 10 21 35 80 

 1529 21 11 30 53 114 

 1530 27 6 32 28 92 

 1531 22 6 33 53 115 

 1532 20 1 26 56 103 

 1533 19 6 33 72 129 

 1534x NP 25 7 28 -16 44 

 1535 20 4 21 56 101 

 1536 28 14 34 59 135 

 1537 27 14 32 72 145 

 1538x NP 18 0 -4 -1 14 

 1539 23 13 28 63 126 

 1540xNNMD 

 1541 20 14 28 62 123 

 1542 14 7 24 61 106 

 1543 26 17 25 66 134 

 1544 24 4 29 56 114 

 1545 19 9 23 69 120 

 1546 36 3 33 66 137 

 1547 20 9 24 47 98 

 1548x NP 16 8 -2 0 23 

 1549 TLL 17 9 20 65 111 

 1550 15 11 16 65 106 

 1551 21 5 17 14 57 

MEAN 22 8 26 56 113

S.D. 5.4 4.2 5.4 14.3 20.0

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:03

Huntingdon Life Sciences 00-4205 PAGE 290 

 

 APPENDIX J P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 2526 26 12 33 76 147 

 2527 17 9 25 39 90 

 2528 24 9 18 71 122 

 2529 15 9 28 60 112 

 2530 21 4 30 62 117 

 2531 15 6 26 61 108 

 2532 21 8 26 58 113 

 2533 32 0 34 65 130 

 2534 17 9 31 59 115 

 2535 16 9 30 55 110 

 2536 HS 

 2537 27 10 28 25 90 

 2538 25 15 26 56 122 

 2539 17 8 26 63 114 

 2540 13 9 23 50 96 

 2541 21 11 28 68 127 

 2542 22 3 32 64 121 

 2543xPNMD 

 2544xNNMD 

 2545 31 11 30 59 131 

 2546 24 16 29 55 124 

 2547 16 4 26 56 102 

 2548 14 9 24 58 106 

 2549xNNMD 

 2550 25 12 23 20 81 

 2551 21 12 24 59 116 

MEAN 21 9 27 56 113

S.D. 5.3 3.8 3.8 13.2 15.5

 N 22 22 22 22 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN 
11-JAN-2010 17:03

Huntingdon Life Sciences 00-4205 PAGE 291 

 

 APPENDIX J P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 3526xNNMD 

 3527 36 13 29 71 149 

 3528 32 14 34 56 136 

 3529 24 10 27 65 126 

 3530xNNMD 

 3531 19 9 17 42 87 

 3532 19 7 28 53 107 

 3533 26 9 34 57 125 

 3534 TLL 19 9 20 6 54 

 3535 23 6 27 68 124 

 3536 17 10 24 57 108 

 3537 28 17 30 24 100 

 3538 23 11 26 49 110 

 3539 33 13 26 53 125 

 3540 26 11 26 24 87 

 3541 15 11 35 63 124 

 3542 25 15 36 25 100 

 3543 15 8 25 70 118 

 3544 25 7 26 47 105 

 3545 31 15 20 56 121 

 3546 19 14 24 74 131 

 3547 15 7 26 51 98 

 3548 21 14 27 71 133 

 3549 15 11 25 74 124 

 3550 20 6 25 70 121 

 3551 24 6 30 77 136 

MEAN 23 11 27 54 115

S.D. 6.0 3.2 4.6 18.7 20.4

 N 24 24 24 24 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:03

Huntingdon Life Sciences 00-4205 PAGE 292 

 

 APPENDIX J P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 4526 27 3 27 46 103 

 4527 11 35 -10 62 98 

 4528 35 3 27 70 135 

 4529 30 9 40 75 153 

 4530xPNMD 

 4531 22 15 13 73 122 

 4532 27 6 29 56 118 

 4533 20 7 28 38 93 

 4534 32 12 29 66 139 

 4535 19 16 31 60 126 

 4536 15 10 26 67 118 

 4537 31 17 33 57 137 

 4538 HS 31 10 36 61 139 

 4539 15 11 27 49 102 

 4540 34 13 19 64 130 

 4541 22 6 30 67 126 

 4542 18 9 25 29 81 

 4543xNNMD 

 4544xNNMD 

 4545 20 8 33 70 130 

 4546 24 14 38 56 132 

 4547 29 10 35 68 142 

 4548 23 4 32 62 120 

 4549 15 11 28 54 109 

 4550 15 8 31 68 123 

 4551x NP 

MEAN 23 11 28 60 122

S.D. 7.0 6.7 10.2 11.4 17.8

 N 22 22 22 22 22

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 

 x=EXCLUDED FROM MEAN 
Huntingdon Life Sciences 00-4205 Page 293

211-DIPE-1G Final Report

Individual Maternal Lactation

Body Weights

Preface Appendix K

No data is presented for the following animals at the stated intervals for the 

following reasons:

Animal No.

Lactation

Days Reason

1549 4 to 28 Total Litter Loss on Lactation Day 2, therefore 

weekly body weights were resumed.

2536 0 to 28 Sacrificed for Humane Reasons on Gestation 

Day 1.

3534 4 to 28 Total Litter Loss on Lactation Day 1, therefore 

weekly body weights were resumed.

4538 21 to 28 Sacrificed for Humane Reasons on Lactation 

Day 14.
11-JAN-2010 17:04

Huntingdon Life Sciences 00-4205 PAGE 294 

 

 APPENDIX K P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 1526 288 311 306 322 315 268 

 1527 299 312 317 340 330 306 

 1528 263 276 273 290 287 269 

 1529 303 322 323 331 340 309 

 1530 343 348 335 351 302 327 

 1531 275 299 309 318 316 295 

 1532 316 329 334 358 345 316 

 1533 326 339 354 366 350 329 

 1534x NP 

 1535 312 259 237 316 315 311 

 1536 337 346 367 380 351 331 

 1537 338 349 340 369 374 342 

 1538x NP 

 1539 349 345 350 371 339 339 

 1540xNNMD 

 1541 320 344 360 363 349 335 

 1542 315 261 236 330 338 300 

 1543 323 340 344 350 342 311 

 1544 339 344 343 366 364 344 

 1545 277 297 301 312 306 284 

 1546 297 307 317 336 339 304 

 1547 317 332 321 329 316 291 

 1548x NP 

 1549 TLL 303 

 1550 285 303 301 324 310 268 

 1551 280 292 311 320 324 292 

MEAN 309 317 318 340 331 308

S.D. 24.6 28.5 35.4 24.0 21.8 24.1

 N 22 21 21 21 21 21

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:04

Huntingdon Life Sciences 00-4205 PAGE 295 

 

 APPENDIX K P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 2526 312 329 333 335 275 303 

 2527 285 315 309 321 324 291 

 2528 288 306 326 351 334 330 

 2529 278 288 305 327 327 292 

 2530 299 324 326 345 341 291 

 2531 306 341 334 357 337 321 

 2532 299 319 320 340 331 309 

 2533 334 356 356 380 372 366 

 2534 303 330 329 342 337 326 

 2535 306 317 313 331 317 299 

 2536 HS 

 2537 315 331 332 343 346 348 

 2538 360 374 388 392 377 343 

 2539 307 320 320 336 327 315 

 2540 284 301 310 316 316 278 

 2541 313 320 330 346 330 310 

 2542 307 317 335 345 332 298 

 2543 PNMD 315 332 343 363 343 322 

 2544xNNMD 

 2545 328 358 366 377 366 331 

 2546 311 335 338 350 335 314 

 2547 265 278 304 323 309 265 

 2548 289 296 307 329 333 308 

 2549xNNMD 

 2550 261 296 307 329 332 293 

 2551 326 352 347 355 343 327 

MEAN 304 323 329 345 334 312

S.D. 22.2 23.4 20.9 19.4 21.0 23.3

 N 23 23 23 23 23 23

-----------------------------------------------------------------------------------------------------------------------------------

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN 
11-JAN-2010 17:04

Huntingdon Life Sciences 00-4205 PAGE 296 

 

 APPENDIX K P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 3526xNNMD 

 3527 316 343 348 357 354 303 

 3528 369 390 391 411 405 355 

 3529 291 321 320 328 324 287 

 3530xNNMD 

 3531 285 304 313 330 326 310 

 3532 299 312 315 342 329 297 

 3533 292 323 321 352 340 305 

 3534 TLL 306 

 3535 281 276 298 318 315 275 

 3536 266 295 309 329 328 304 

 3537 304 319 320 346 330 288 

 3538 342 339 337 355 347 326 

 3539 350 350 352 362 374 343 

 3540 307 316 329 363 359 320 

 3541 332 349 356 360 351 339 

 3542 323 334 334 347 354 316 

 3543 286 294 304 330 333 291 

 3544 271 297 308 326 322 287 

 3545 333 333 344 360 355 336 

 3546 327 347 360 370 366 335 

 3547 265 270 291 311 308 275 

 3548 318 340 340 362 376 319 

 3549 280 300 308 318 322 289 

 3550 274 305 312 327 329 309 

 3551 316 330 334 365 355 318 

MEAN 306 321 328 346 343 310

S.D. 27.8 27.3 23.4 22.6 23.1 22.3

 N 24 23 23 23 23 23

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:04

Huntingdon Life Sciences 00-4205 PAGE 297 

 

 APPENDIX K P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

 ---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

 4526 273 302 298 308 312 288 

 4527 293 295 308 328 327 306 

 4528 311 320 323 335 350 308 

 4529 353 356 371 401 388 353 

 4530 PNMD 274 328 328 340 327 307 

 4531 323 331 346 360 356 317 

 4532 282 295 312 337 331 300 

 4533 282 307 309 327 333 303 

 4534 301 320 327 349 336 300 

 4535 318 342 345 360 361 352 

 4536 323 340 347 372 372 345 

 4537 330 341 338 360 348 312 

 4538 HS 319 320 345 362 

 4539 271 290 302 316 315 300 

 4540 355 374 383 390 392 359 

 4541 299 322 319 349 324 290 

 4542 277 286 297 321 303 268 

 4543xNNMD 

 4544xNNMD 

 4545 307 311 317 344 347 293 

 4546 328 354 364 401 393 352 

 4547 372 372 386 399 387 365 

 4548 258 278 297 302 313 271 

 4549 297 309 311 316 323 280 

 4550 252 283 287 318 316 282 

 4551x NP 

MEAN 304 321 329 348 343 311

S.D. 31.5 27.5 28.2 30.1 28.3 29.9

 N 23 23 23 23 22 22

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 

 x=EXCLUDED FROM MEAN 
Huntingdon Life Sciences 00-4205 Page 298

211-DIPE-1G Final Report

Individual Maternal Lactation

Body Weight Gain

Preface Appendix L

No data is presented for the following animals at the stated intervals for the 

following reasons:

Animal No.

Lactation

Days Reason

1549 1-4 through 

21-28 and 

1-28

Total Litter Loss on Lactation Day 2, therefore 

weekly body weights were resumed.

2536 1-4 through 

21-28 and 

1-28

Sacrificed for Humane Reasons on Gestation 

Day 1.

3534 1-4 through 

21-28 and 

1-28

Total Litter Loss on Lactation Day 1, therefore 

weekly body weights were resumed.

4538 14-21 

through 

21-28 and 

1-28

Sacrificed for Humane Reasons on Lactation 

Day 14.
11-JAN-2010 17:06

Huntingdon Life Sciences 00-4205 PAGE 299 

 

 APPENDIX L P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 1526 23 -5 17 -7 -47 -19 

 1527 13 5 23 -10 -24 7 

 1528 13 -3 17 -3 -18 5 

 1529 19 2 8 9 -31 6 

 1530 5 -14 16 -49 25 -16 

 1531 24 10 10 -2 -21 21 

 1532 12 5 24 -13 -29 -1 

 1533 13 15 12 -16 -21 3 

 1534x NP 

 1535 -52 -22 79 -1 -4 -1 

 1536 9 21 13 -29 -19 -6 

 1537 11 -9 29 5 -33 3 

 1538x NP 

 1539 -4 5 22 -32 0 -10 

 1540xNNMD 

 1541 23 16 4 -14 -15 14 

 1542 -53 -25 94 8 -38 -14 

 1543 17 4 6 -7 -32 -13 

 1544 5 -1 23 -2 -20 5 

 1545 21 3 11 -6 -22 7 

 1546 10 10 19 4 -35 7 

 1547 15 -11 9 -13 -25 -26 

 1548x NP 

 1549 TLL 

 1550 18 -2 23 -14 -42 -17 

 1551 13 18 9 4 -31 13 

MEAN 7 1 22 -9 -23 -1 

S.D. 21.1 12.5 22.5 14.2 15.8 12.5

 N 21 21 21 21 21 21

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:06

Huntingdon Life Sciences 00-4205 PAGE 300 

 

 APPENDIX L P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 2526 17 4 2 -60 28 -9 

 2527 31 -7 12 3 -33 6 

 2528 18 20 24 -16 -4 42 

 2529 10 18 21 1 -36 14 

 2530 25 2 18 -4 -50 -8 

 2531 35 -6 23 -20 -16 16 

 2532 21 0 21 -10 -22 11 

 2533 22 0 24 -7 -7 31 

 2534 27 -1 13 -5 -11 23 

 2535 12 -4 18 -14 -18 -7 

 2536 HS 

 2537 16 0 11 3 3 33 

 2538 14 15 4 -15 -34 -17 

 2539 13 0 16 -9 -12 8 

 2540 17 9 6 0 -38 -5 

 2541 7 10 16 -16 -20 -3 

 2542 10 18 10 -14 -34 -9 

 2543 PNMD 17 12 20 -20 -21 7 

 2544xNNMD 

 2545 30 8 12 -11 -36 2 

 2546 24 4 12 -15 -21 3 

 2547 13 26 19 -14 -44 0 

 2548 7 11 22 4 -25 19 

 2549xNNMD 

 2550 35 11 22 3 -39 32 

 2551 27 -6 9 -13 -16 2 

MEAN 19 6 15 -11 -22 8

S.D. 8.5 9.1 6.6 13.2 17.3 15.8

 N 23 23 23 23 23 23

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN 
11-JAN-2010 17:06

Huntingdon Life Sciences 00-4205 PAGE 301 

 

 APPENDIX L P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 3526xNNMD 

 3527 28 4 10 -4 -50 -13 

 3528 21 1 20 -6 -50 -14 

 3529 30 0 8 -4 -37 -4 

 3530xNNMD 

 3531 19 9 17 -4 -16 25 

 3532 13 3 27 -14 -32 -2 

 3533 30 -2 31 -12 -35 13 

 3534 TLL 

 3535 -5 22 20 -4 -40 -6 

 3536 29 13 20 0 -24 38 

 3537 15 1 26 -16 -42 -16 

 3538 -3 -2 19 -9 -21 -16 

 3539 0 3 10 12 -31 -7 

 3540 9 13 34 -4 -39 13 

 3541 17 7 4 -9 -12 7 

 3542 11 0 12 7 -38 -7 

 3543 8 10 26 3 -42 5 

 3544 26 12 18 -5 -35 16 

 3545 0 10 16 -5 -19 3 

 3546 20 12 11 -4 -31 7 

 3547 5 21 20 -3 -32 11 

 3548 23 0 22 14 -57 1 

 3549 20 8 10 5 -34 8 

 3550 31 7 15 2 -20 35 

 3551 14 4 30 -9 -38 1 

MEAN 16 7 18 -3 -34 4

S.D. 11.2 6.7 8.0 7.5 11.3 14.8

 N 23 23 23 23 23 23

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:06

Huntingdon Life Sciences 00-4205 PAGE 302 

 

 APPENDIX L P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

FEMALE# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 4526 29 -3 9 4 -24 15 

 4527 3 13 20 -1 -22 13 

 4528 9 3 13 15 -42 -4 

 4529 3 15 30 -13 -35 0 

 4530 PNMD 54 0 12 -13 -19 34 

 4531 7 15 15 -4 -38 -6 

 4532 13 17 26 -6 -31 18 

 4533 25 2 18 5 -30 20 

 4534 19 7 22 -14 -35 -1 

 4535 23 3 15 1 -10 33 

 4536 16 7 25 0 -26 22 

 4537 11 -3 22 -12 -36 -18 

 4538 HS 1 25 18 

 4539 20 12 13 0 -15 30 

 4540 19 9 7 2 -33 4 

 4541 22 -2 30 -25 -35 -10 

 4542 8 11 24 -18 -35 -10 

 4543xNNMD 

 4544xNNMD 

 4545 4 5 27 4 -55 -14 

 4546 26 10 37 -8 -42 24 

 4547 0 14 13 -13 -22 -6 

 4548 20 20 5 10 -42 13 

 4549 13 2 5 8 -43 -16 

 4550 31 4 31 -2 -34 30 

 4551x NP 

MEAN 16 8 19 -4 -32 8

S.D. 12.4 7.5 8.9 9.9 10.5 17.1

 N 23 23 23 22 22 22

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 

 x=EXCLUDED FROM MEAN 
Huntingdon Life Sciences 00-4205 Page 303

211-DIPE-1G Final Report

Individual Feed Consumption

(Premating/Mating/Postmating)

Preface Appendix M

No data is presented for the following animals at the stated intervals for the 

following reasons:

Animal No. Day(s) Reason

3043 28 to 112 Sacrificed for Humane Reasons on Day 23

3044 112 Found Dead on Day 110

1540 112 Terminal Sacrifice on Day 108

1549 91 to 98 Total Litter Loss on Lactation Day 2, therefore weekly 

feed consumption was resumed.

2543 98 to 112 Although no evidence of mating was observed, this 

female delivered on Day 98. See Lactation Feed 

Consumption.

2544 112 Terminal Sacrifice on Day 108

2549 112 Terminal Sacrifice on Day 108

3526 112 Terminal Sacrifice on Day 108

3530 112 Terminal Sacrifice on Day 108

3534 91 to 98 Total Litter Loss on Lactation Day 1, therefore weekly 

feed consumption was resumed.

4530 98 to 112 Although no evidence of mating was observed, this 

female delivered on Day 94. See Lactation Feed 

Consumption.

4543 112 Terminal Sacrifice on Day 108

4544 112 Terminal Sacrifice on Day 108

4551 112 Terminal Sacrifice on Day 108
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 304 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 123 108 99 96 84 75 74 70 58 56 55 49 53 46 49 

 1027 121 98 89 77 70 66 64 58 56 54 50 47 45 47 46 

 1028 122 98 88 78 72 67 66 61 57 53 55 48 47 46 48 

 1029 123 102 86 75 69 66 64 61 46 56 51 50 51 52 49 

 1030 122 99 88 78 71 65 61 59 56 52 49 48 48 47 47 

 1031 125 101 89 78 69 66 58 58 58 53 52 52 47 47 49 

 1032 120 100 84 76 58 64 62 58 58 54 55 47 49 48 48 

 1033 130 97 83 71 71 69 67 63 62 53 35 51 50 49 40 

 1034 120 96 85 74 69 64 63 59 59 58 53 48 47 45 46 

 1035 118 93 80 71 64 62 61 59 56 53 53 48 46 50 46 

 1036 125 103 94 82 67 64 63 61 59 55 52 49 52 46 47 

 1037 129 105 89 81 72 69 64 BF 58 56 55 51 50 49 52 

 1038 121 94 85 74 70 64 60 60 60 51 51 48 46 44 47 

 1039 129 100 87 77 71 67 65 60 59 53 56 52 46 49 46 

 1040 123 103 92 78 68 65 62 60 57 50 54 47 47 43 41 

 1041 125 102 92 78 72 66 62 59 56 53 57 46 48 47 47 

 1042 121 102 93 82 75 70 68 62 60 55 56 51 49 48 47 

 1043 129 108 89 76 69 71 67 70 64 62 61 53 54 53 55 

 1044 132 105 93 84 72 69 64 62 51 58 52 49 47 46 49 

 1045 117 95 89 81 64 65 65 61 59 57 56 49 47 46 46 

 1046 134 105 96 83 68 65 70 69 64 62 64 58 53 56 50 

 1047 121 100 93 87 73 70 65 65 62 59 57 49 49 54 52 

 1048 117 94 87 73 68 67 64 63 57 55 53 49 48 48 47 

 1049 124 100 89 76 72 70 64 64 61 59 54 52 50 47 46 

 1050 122 98 IW 81 68 67 27 83 67 59 56 50 52 47 47 

 1051 127 103 89 80 74 66 65 64 62 57 54 51 50 47 47 

MEAN 124 100 89 79 70 67 63 63 59 55 54 50 49 48 47

S.D. 4.6 4.1 4.2 5.3 4.5 2.9 8.0 5.5 4.1 3.2 5.0 2.4 2.5 3.0 3.0

 N 26 26 25 26 26 26 26 25 26 26 26 26 26 26 26

 BF=Broken Feeder IW=Invalid Weight: Feed left greater than feed fed weight 
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 305 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 130 100 86 74 69 67 64 61 58 57 55 47 48 52 50 

 2027 124 94 81 71 67 64 59 58 57 53 51 47 47 48 45 

 2028 130 100 87 77 69 65 62 56 56 54 55 46 46 46 45 

 2029 122 94 84 76 71 64 64 60 56 58 52 44 43 47 48 

 2030 130 105 90 83 76 70 66 63 49 54 54 49 45 46 45 

 2031 133 106 92 83 76 71 67 60 60 59 55 51 49 53 51 

 2032 122 100 87 77 70 64 62 56 54 54 53 45 44 45 47 

 2033 138 105 90 79 70 64 65 62 59 57 55 50 47 49 47 

 2034 121 97 85 73 73 67 60 57 47 52 50 44 43 43 43 

 2035 122 98 83 72 68 63 64 60 49 54 51 48 48 49 48 

 2036 131 115 97 81 78 68 68 67 63 60 58 55 58 56 54 

 2037 121 93 86 75 69 68 65 62 57 55 51 45 45 43 45 

 2038 128 102 82 74 69 63 60 57 53 53 52 46 48 45 44 

 2039 122 97 88 80 77 65 63 61 50 55 56 49 48 45 46 

 2040 124 97 83 74 71 67 63 62 58 52 53 48 48 46 45 

 2041 117 99 84 73 69 65 61 57 48 52 51 47 45 44 43 

 2042 122 95 85 77 70 65 63 57 53 51 50 46 45 43 42 

 2043 121 94 86 79 72 68 66 63 41 56 49 44 45 45 45 

 2044 129 99 85 77 67 66 65 63 57 54 54 47 45 44 44 

 2045 123 96 84 72 68 63 62 61 49 52 51 47 46 45 46 

 2046 132 100 85 76 71 66 61 60 57 56 57 51 51 49 47 

 2047 132 103 89 76 69 63 62 61 57 56 52 46 44 46 45 

 2048 128 99 87 77 67 65 66 60 60 56 52 50 48 48 43 

 2049 124 99 87 78 74 68 65 62 56 56 52 44 46 46 44 

 2050 129 105 87 78 72 68 60 60 58 55 54 48 47 48 46 

 2051 125 98 83 77 71 66 64 62 59 56 55 48 48 47 46 

MEAN 126 99 86 77 71 66 63 60 55 55 53 47 47 47 46

S.D. 5.0 4.8 3.4 3.1 3.2 2.1 2.4 2.6 5.1 2.4 2.2 2.7 2.9 3.2 2.6

 N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 306 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 132 100 95 96 80 71 68 64 52 55 54 52 45 47 49 

 3027 128 101 87 75 75 68 63 60 57 54 51 52 50 50 50 

 3028 121 98 86 76 69 67 61 61 58 53 54 48 47 45 46 

 3029 128 99 89 78 70 67 65 63 60 57 54 48 49 50 49 

 3030 129 100 83 75 69 64 57 57 56 50 50 45 44 43 43 

 3031 138 108 87 75 71 69 65 SF 59 56 53 49 51 48 48 

 3032 120 96 85 75 66 66 63 61 54 53 51 49 46 51 49 

 3033 126 105 90 76 69 67 65 61 57 55 52 54 51 51 49 

 3034 125 99 89 76 70 70 64 60 58 53 58 49 48 47 48 

 3035 122 97 85 74 68 66 62 57 54 52 50 49 46 49 50 

 3036 131 98 86 74 68 67 65 59 58 52 52 48 47 51 50 

 3037 129 106 91 77 70 68 67 64 59 58 55 50 49 55 53 

 3038 123 98 85 75 65 67 65 65 59 53 55 50 50 53 50 

 3039 139 95 84 75 65 68 64 66 52 52 55 49 50 48 45 

 3040 139 103 90 79 72 67 63 63 50 45 54 50 48 47 47 

 3041 BF 102 90 77 69 67 66 64 59 54 54 49 49 47 46 

 3042 126 98 87 73 66 64 62 61 58 54 54 49 50 51 48 

 3043 133 104 91 39 

 3044 121 93 80 71 65 65 61 61 58 54 52 47 46 39 

 3045 120 97 85 75 63 66 60 62 56 59 53 52 50 50 47 

 3046 128 98 83 73 66 67 63 61 57 50 52 48 48 50 48 

 3047 129 100 87 78 73 68 67 62 58 56 57 52 52 51 47 

 3048 129 100 87 78 69 66 65 64 60 59 55 48 50 49 46 

 3049 130 100 86 75 71 70 67 63 60 55 57 50 49 50 46 

 3050 134 101 90 79 73 70 66 62 55 48 58 49 49 50 46 

 3051 129 98 87 76 72 71 67 64 56 53 55 49 45 48 47 

MEAN 128 100 87 75 69 67 64 62 57 54 54 49 48 49 48

S.D. 5.6 3.4 3.2 8.6 3.7 1.9 2.6 2.3 2.7 3.2 2.2 1.9 2.1 3.2 2.2

 N 25 26 26 26 25 25 25 24 25 25 25 25 25 25 24

 SF=Spilled Feeder BF=Broken Feeder 
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 307 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 130 99 85 49 65 65 61 59 49 51 52 45 44 45 46 

 4027 123 95 82 73 68 68 60 59 56 55 51 51 48 49 48 

 4028 125 100 91 82 SF 73 70 69 57 54 55 50 48 47 45 

 4029 121 97 84 73 67 65 57 57 54 51 50 47 46 44 43 

 4030 128 96 83 71 67 63 60 57 53 53 50 45 44 46 46 

 4031 135 99 85 74 65 64 61 62 57 54 55 47 49 51 47 

 4032 131 93 84 74 67 64 61 59 57 43 52 46 46 47 44 

 4033 140 97 87 85 68 66 62 60 57 55 55 49 48 50 48 

 4034 132 100 88 74 65 62 59 57 59 54 53 48 50 50 48 

 4035 129 97 83 73 69 65 60 59 53 37 54 53 45 47 51 

 4036 123 92 84 75 69 64 60 58 57 53 53 47 44 49 46 

 4037 135 101 84 87 66 62 57 57 56 52 52 50 47 48 48 

 4038 134 96 88 78 73 68 65 62 58 53 53 47 48 48 47 

 4039 123 97 75 85 72 67 62 62 61 59 59 54 54 54 50 

 4040 143 109 88 85 85 73 66 67 55 59 57 53 53 51 51 

 4041 133 100 86 78 71 68 68 69 62 63 54 51 48 50 49 

 4042 125 96 85 77 72 66 62 61 58 56 53 46 47 46 46 

 4043 118 96 82 71 69 62 63 58 57 53 49 46 46 49 45 

 4044 125 93 82 72 68 66 64 64 58 56 55 50 47 48 47 

 4045 136 107 91 81 72 68 65 65 62 61 59 51 50 51 40 

 4046 127 96 85 74 70 68 64 60 54 55 53 47 47 45 43 

 4047 127 98 83 74 67 59 58 60 58 56 46 47 47 50 47 

 4048 136 99 91 83 76 68 68 64 59 40 53 49 50 49 45 

 4049 135 98 87 81 73 68 64 61 59 58 16 53 52 51 50 

 4050 133 99 89 83 79 69 67 65 60 59 54 53 53 54 50 

 4051 135 104 88 77 74 70 65 65 61 56 52 49 46 48 45 

MEAN 130 98 85 76 70 66 63 61 57 54 52 49 48 49 47

S.D. 6.1 4.0 3.6 7.3 4.7 3.2 3.4 3.7 3.0 5.8 7.8 2.6 2.7 2.5 2.7

 N 26 26 26 26 25 26 26 26 26 26 26 26 26 26 26

 SF=Spilled Feeder 
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 308 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 124 96 83 80 79 76 74 73 77 66 63 

 1527 121 97 86 80 80 73 72 69 66 63 64 

 1528 119 100 99 81 78 78 78 74 70 71 71 

 1529 120 98 91 82 81 83 80 74 69 65 65 

 1530 124 101 98 89 89 82 75 78 69 57 57 

 1531 115 96 89 83 88 82 80 77 68 69 66 

 1532 125 99 95 91 82 86 88 85 70 70 66 

 1533 132 106 96 86 83 79 80 75 67 63 65 

 1534 127 99 98 86 86 78 77 76 65 62 66 

 1535 139 107 97 86 84 80 78 78 68 63 62 

 1536 131 105 100 91 97 79 80 78 70 65 70 

 1537 141 105 95 87 86 82 79 76 71 66 70 

 1538 122 99 95 86 84 81 74 71 69 61 62 

 1539 136 105 89 82 82 78 71 68 65 60 59 

 1540 111 93 93 85 82 62 82 77 71 64 66 57 54 57 

 1541 140 110 101 92 87 IW 82 80 72 73 68 

 1542 132 106 101 88 83 79 77 75 70 70 61 

 1543 134 125 107 93 91 90 82 78 74 68 68 

 1544 137 113 108 95 96 85 77 80 71 63 67 

 1545 125 108 101 92 84 82 80 78 76 71 71 

 1546 140 118 100 96 82 81 78 83 76 74 71 

 1547 130 111 104 89 83 78 76 75 71 68 67 

 1548 132 105 97 86 79 76 71 72 66 64 64 

 1549 99 85 83 76 69 77 68 63 63 63 56 51 110 

 1550 128 105 102 89 83 81 77 74 72 69 69 

 1551 128 102 93 83 79 77 32 105 78 69 65 

MEAN 127 104 96 87 84 80 76 77 70 66 65 57 54 54 110

S.D. 9.8 8.1 6.5 4.9 5.6 5.1 9.9 7.4 3.8 4.2 4.1 0.0 0.0 4.4 0.0

 N 26 26 26 26 26 25 26 26 26 26 26 1 1 2 1

 IW=Invalid Weight: Feed left greater than feed fed weight 
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 309 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 135 100 91 86 78 81 74 72 74 64 64 

 2527 130 109 92 86 87 81 78 75 SF 69 61 

 2528 130 102 94 83 85 82 82 76 75 73 72 

 2529 125 94 87 82 78 80 76 73 70 68 61 

 2530 137 107 99 93 88 80 80 81 72 69 68 

 2531 130 101 89 82 81 80 74 69 67 65 59 

 2532 139 97 87 79 81 76 75 73 69 61 62 

 2533 129 98 92 85 84 73 69 69 69 70 60 

 2534 134 106 93 89 90 88 75 SF 68 72 68 

 2535 129 98 85 81 76 76 74 73 68 64 66 

 2536 123 96 86 78 80 73 72 69 70 65 61 

 2537 139 100 89 84 81 79 73 73 67 64 59 

 2538 144 117 101 89 85 79 78 72 70 63 53 

 2539 132 102 94 86 79 74 73 70 67 58 57 

 2540 138 110 90 82 81 75 70 69 68 67 63 

 2541 122 92 52 114 87 80 73 72 72 64 62 

 2542 142 100 87 82 80 71 71 66 67 63 65 

 2543 132 103 88 82 76 73 73 71 69 68 66 70 

 2544 139 114 106 104 100 SF 92 86 83 74 67 64 53 58 

 2545 143 110 101 92 79 84 80 79 74 72 67 

 2546 153 113 97 89 80 77 76 78 74 69 63 

 2547 135 105 91 82 77 72 74 70 66 65 63 

 2548 132 103 100 89 83 75 76 76 71 68 69 

 2549 136 94 91 82 82 72 71 71 69 65 66 57 53 54 

 2550 145 95 97 99 91 SF 90 88 85 80 82 

 2551 138 117 108 80 80 79 74 68 72 68 60 

MEAN 135 103 92 87 83 78 76 74 71 67 64 64 53 56 

S.D. 7.2 7.1 10.1 8.1 5.5 4.2 5.5 5.4 4.8 4.6 5.6 6.6 0.4 2.6 

 N 26 26 26 26 26 24 26 25 25 26 26 3 2 2 

 SF=Spilled Feeder 
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 310 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 125 98 92 84 78 80 76 74 69 62 61 54 51 51 

 3527 125 102 94 83 85 80 76 76 73 65 63 

 3528 129 95 92 84 83 85 78 79 70 64 62 

 3529 124 102 87 71 72 72 72 65 70 63 64 

 3530 135 95 85 82 81 76 75 67 62 61 56 55 57 52 

 3531 133 102 91 83 80 79 76 74 69 64 66 

 3532 133 107 95 84 89 85 85 83 71 70 69 

 3533 134 105 97 81 88 83 82 82 77 64 65 

 3534 SF SF SF 91 77 77 85 81 61 74 69 57 130 

 3535 121 100 87 88 83 78 80 77 81 63 68 

 3536 130 105 89 85 89 84 82 82 71 94 73 

 3537 130 103 107 88 86 83 84 82 76 71 74 

 3538 134 103 99 89 83 84 82 73 76 73 67 

 3539 135 119 95 88 84 78 79 75 78 68 66 

 3540 133 100 92 85 81 79 81 78 69 64 60 

 3541 128 100 97 90 83 84 83 74 72 69 67 

 3542 133 104 93 90 84 74 83 82 79 69 66 

 3543 142 SF 86 86 82 74 72 70 70 63 61 

 3544 121 106 92 86 84 83 79 80 74 69 72 

 3545 132 SF 99 89 79 77 84 75 82 70 75 

 3546 127 SF 89 87 SF 76 77 75 68 63 62 

 3547 133 106 96 92 SF 77 80 75 71 65 64 

 3548 135 109 96 92 84 76 76 78 69 68 64 

 3549 127 109 103 88 85 78 80 73 69 67 64 

 3550 129 117 100 91 85 89 85 78 75 69 66 

 3551 150 115 101 91 87 85 80 81 80 70 65 

MEAN 131 105 94 86 83 80 80 76 72 68 66 54 54 54 130

S.D. 6.2 6.3 5.6 4.4 4.0 4.2 3.9 4.6 5.3 6.4 4.4 0.4 4.6 3.2 0.0

 N 25 22 25 26 24 26 26 26 26 26 26 2 2 3 1

 SF=Spilled Feeder 
11-JAN-2010 17:07

Huntingdon Life Sciences 00-4205 PAGE 311 

 

 APPENDIX M P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY) 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 125 95 86 79 78 77 77 71 66 65 65 

 4527 127 104 91 90 84 80 73 71 71 63 63 

 4528 126 94 85 76 78 75 76 70 66 64 65 

 4529 145 102 94 92 98 82 93 85 74 89 67 

 4530 129 99 92 83 54 77 77 78 74 65 64 59 

 4531 120 87 79 78 73 72 67 72 57 63 63 

 4532 120 94 86 79 82 78 74 75 72 69 67 

 4533 127 95 SF 76 81 79 72 73 69 98 64 

 4534 136 101 87 76 80 79 SF 77 74 67 68 

 4535 127 96 86 84 75 73 70 69 64 64 60 

 4536 124 103 91 81 77 73 73 72 66 60 63 

 4537 135 106 91 84 82 76 72 72 68 60 60 

 4538 145 114 97 85 86 79 78 82 74 76 65 

 4539 118 97 89 80 83 83 79 75 71 76 68 

 4540 138 109 100 80 90 78 70 72 73 65 63 

 4541 129 SF 108 77 83 77 75 75 77 72 70 

 4542 125 102 95 88 88 82 79 72 71 80 SF 

 4543 134 97 88 89 79 72 73 75 67 59 66 57 52 52 

 4544 134 104 91 88 80 78 75 74 71 63 61 58 58 57 

 4545 138 104 92 87 82 77 75 72 73 70 65 

 4546 126 97 86 80 76 75 72 69 65 66 56 

 4547 142 112 98 88 86 79 75 76 74 68 71 

 4548 136 106 92 84 82 73 75 75 69 90 67 

 4549 131 89 83 83 80 74 78 76 63 63 64 

 4550 130 104 82 80 80 83 75 70 73 70 62 

 4551 141 106 100 87 89 82 81 74 79 70 75 60 56 55 

MEAN 131 101 91 83 81 77 75 74 70 70 65 59 56 55 

S.D. 7.6 6.6 6.6 4.6 7.6 3.3 4.9 3.6 4.9 9.9 4.0 1.7 3.0 2.7 

 N 26 25 25 26 26 26 25 26 26 26 25 4 3 3 

 SF=Spilled Feeder 
Huntingdon Life Sciences 00-4205 Page 312

211-DIPE-1G Final Report

Individual Gestation Feed Consumption

Preface Appendix N

No data is presented for the following animal at the stated intervals for the 

following reasons:

Animal No.

Gestation

Days Reason

2536 0-4 through 

14-20 and 

0-20

Sacrificed for Humane Reasons on Gestation 

Day 1 
11-JAN-2010 17:11

Huntingdon Life Sciences 00-4205 PAGE 313 

 

 APPENDIX N P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 1526 66 70 65 64 67 

 1527 67 72 68 63 67 

 1528 74 80 73 64 73 

 1529 73 74 72 63 71 

 1530 61 65 69 61 64 

 1531 78 73 76 62 72 

 1532 65 75 68 62 67 

 1533 73 73 72 67 71 

 1534x NP 69 68 76 57 68 

 1535 67 67 64 60 65 

 1536 81 78 78 65 75 

 1537 79 76 72 61 72 

 1538x NP 64 58 57 49 57 

 1539 71 80 68 62 70 

 1540xNNMD 

 1541 69 74 71 65 70 

 1542 71 76 71 65 71 

 1543 71 81 73 62 72 

 1544 77 75 76 59 72 

 1545 76 80 72 70 75 

 1546 79 79 77 69 76 

 1547 69 75 73 72 72 

 1548x NP 66 69 62 57 63 

 1549 TLL 68 71 65 61 66 

 1550 78 79 70 65 73 

 1551 69 71 66 66 68 

MEAN 72 75 71 64 70

S.D. 5.3 4.3 3.9 3.4 3.3

 N 22 22 22 22 22

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 
11-JAN-2010 17:11

Huntingdon Life Sciences 00-4205 PAGE 314 

 

 APPENDIX N P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 2526 78 80 76 60 74 

 2527 71 71 67 53 65 

 2528 79 79 73 63 73 

 2529 71 73 72 62 69 

 2530 75 75 71 62 71 

 2531 62 64 65 57 62 

 2532 63 71 70 61 66 

 2533 71 70 70 65 69 

 2534 63 72 70 63 67 

 2535 63 67 66 61 64 

 2536 HS 

 2537 61 68 69 58 64 

 2538 67 70 63 55 64 

 2539 67 63 68 64 65 

 2540 72 71 70 59 68 

 2541 81 76 71 61 72 

 2542 SF 72 73 67 71 

 2543xPNMD 

 2544xNNMD 

 2545 77 74 74 66 73 

 2546 75 88 81 60 76 

 2547 68 65 66 62 66 

 2548 78 84 76 66 76 

 2549xNNMD 

 2550 95 88 77 67 82 

 2551 74 74 66 59 68 

MEAN 72 73 71 61 69

S.D. 8.2 7.0 4.4 3.8 4.9

 N 21 22 22 22 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN 

 SF=Spilled Feeder 
11-JAN-2010 17:11

Huntingdon Life Sciences 00-4205 PAGE 315 

 

 APPENDIX N P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 3526xNNMD 

 3527 81 79 72 59 73 

 3528 73 76 71 BF 74 

 3529 73 77 72 60 71 

 3530xNNMD 

 3531 72 69 66 52 65 

 3532 79 85 77 68 77 

 3533 72 71 70 59 68 

 3534 TLL 64 80 71 68 71 

 3535 71 70 67 59 67 

 3536 71 75 71 66 71 

 3537 85 96 77 60 79 

 3538 68 71 66 54 65 

 3539 75 76 70 59 70 

 3540 76 75 71 59 70 

 3541 73 77 72 56 70 

 3542 76 75 76 62 72 

 3543 72 70 66 62 68 

 3544 83 78 72 62 74 

 3545 83 87 71 64 76 

 3546 65 73 68 60 66 

 3547 74 71 76 61 71 

 3548 71 74 70 67 71 

 3549 72 71 65 60 67 

 3550 79 75 70 61 71 

 3551 77 78 74 68 74 

MEAN 74 76 71 61 71

S.D. 5.3 6.2 3.5 4.3 3.7

 N 24 24 24 23 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

 BF=Broken Feeder 
11-JAN-2010 17:11

Huntingdon Life Sciences 00-4205 PAGE 316 

 

 APPENDIX N P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL GESTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF GESTATION

ANIMAL# 0 - 4 4 - 7 7 - 14 14 - 20 0 - 20

-----------------------------------------------------------------------------------------------------------------------------------

 4526 79 73 72 60 71 

 4527 67 59 55 58 60 

 4528 73 66 67 60 66 

 4529 76 81 73 67 74 

 4530xPNMD 

 4531 72 74 57 60 66 

 4532 77 79 76 70 75 

 4533 67 69 67 66 67 

 4534 89 80 73 66 77 

 4535 65 74 70 57 66 

 4536 66 69 64 59 64 

 4537 74 82 75 60 73 

 4538 HS 77 80 76 63 74 

 4539 75 77 75 66 73 

 4540 76 75 66 61 70 

 4541 75 73 73 68 72 

 4542 69 72 68 65 68 

 4543xNNMD 

 4544xNNMD 

 4545 67 64 68 64 66 

 4546 66 72 69 56 66 

 4547 70 74 72 60 69 

 4548 75 68 76 60 70 

 4549 69 78 73 60 70 

 4550 69 71 71 66 69 

 4551x NP 

MEAN 72 73 70 62 69

S.D. 5.8 5.9 5.5 3.9 4.1

 N 22 22 22 22 22

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 

 x=EXCLUDED FROM MEAN 
Huntingdon Life Sciences 00-4205 Page 317

211-DIPE-1G Final Report

Individual Maternal Lactation

Feed Consumption

Preface Appendix O

No data is presented for the following animals at the stated intervals for the 

following reasons:

Animal No.

Lactation

Days Reason

1549 1-4 through 

21-28 and 

1-28

Total Litter Loss on Lactation Day 2, therefore 

weekly feed consumption was resumed.

2536 1-4 through 

21-28 and 

1-28

Sacrificed for Humane Reasons on Gestation 

Day 1.

3534 1-4 through 

21-28 and 

1-28

Total Litter Loss on Lactation Day 1, therefore 

weekly feed consumption was resumed.

4538 14-21 

through 

21-28 and 

1-28

Sacrificed for Humane Reasons on Lactation 

Day 14.
11-JAN-2010 17:14

Huntingdon Life Sciences 00-4205 PAGE 318 

 

 APPENDIX 0 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 1526 108 120 146 193 372 188 

 1527 98 116 143 194 371 184 

 1528 75 96 123 173 307 155 

 1529 97 97 129 200 353 175 

 1530 57 74 98 136 248 122 

 1531 87 114 140 165 337 168 

 1532 96 111 142 181 EF 132 

 1533 104 114 130 173 352 175 

 1534x NP 

 1535 41 IW1 135 164 306 162 

 1536 96 116 134 177 EF 131 

 1537 116 118 147 197 EF 144 

 1538x NP 

 1539 76 95 131 177 368 169 

 1541 106 119 145 181 353 181 

 1542 65 IW1 153 181 318 179 

 1543 101 114 139 185 142 136 

 1544 88 98 134 185 372 176 

 1545 104 116 146 187 372 185 

 1546 118 125 145 202 EF 147 

 1547 72 80 103 137 266 132 

 1548x NP 

 1549 TLL 

 1550 85 107 143 177 366 176 

 1551 91 117 138 175 314 167 

MEAN 90 108 135 178 324 161

S.D. 19.6 14.1 13.7 17.3 60.4 20.9

 N 21 19 21 21 17 21

 NP=NOT PREGNANT TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

 EF=Empty Feeder IW1=Invalid Weight: Water access problem 
11-JAN-2010 17:14

Huntingdon Life Sciences 00-4205 PAGE 319 

 

 APPENDIX 0 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION 

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 2526 92 115 138 201 414 192 

 2527 114 112 139 190 364 184 

 2528 85 114 144 172 305 164 

 2529 94 117 137 187 329 173 

 2530 105 124 144 202 EF 144 

 2531 105 117 138 180 343 176 

 2532 78 98 138 195 348 172 

 2533 87 104 141 189 EF 130 

 2534 97 113 139 IW IW 116 

 2535 96 103 132 179 419 186 

 2536 HS 

 2537 61 72 88 118 190 106 

 2538 69 91 104 145 294 141 

 2539 82 97 123 182 317 160 

 2540 106 124 140 186 387 189 

 2541 96 112 133 180 366 177 

 2542 84 110 132 180 EF 126 

 2543 PNMD 121 129 151 219 374 199 

 2545 79 101 130 164 332 161 

 2546 98 118 143 205 378 188 

 2547 98 128 151 177 EF 139 

 2548 118 125 158 200 EF 150 

 2550 105 117 151 189 250 163 

 2551 79 100 129 194 382 177 

MEAN 93 111 136 183 341 161

S.D. 15.0 13.4 15.2 21.2 58.2 25.8

 N 23 23 23 22 17 23

PNMD=PREGNANT, NO MATING DATE HS=HUMANE SACRIFICE 

 EF=Empty Feeder IW=Invalid Weight: Feed left greater than feed fed weight 
11-JAN-2010 17:14

Huntingdon Life Sciences 00-4205 PAGE 320 

 

 APPENDIX 0 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 3527 99 115 128 172 316 166 

 3528 93 104 125 164 301 158 

 3529 123 117 147 198 377 192 

 3531 89 114 140 201 294 168 

 3532 105 131 147 204 334 184 

 3533 97 113 140 171 284 161 

 3534 TLL 

 3535 100 103 126 165 276 154 

 3536 105 128 155 205 332 185 

 3537 114 128 158 210 406 203 

 3538 73 94 126 176 317 157 

 3539 93 146 149 172 392 190 

 3540 100 92 147 193 EF 133 

 3541 105 125 145 208 387 194 

 3542 94 108 142 204 360 182 

 3543 104 118 141 206 355 185 

 3544 111 133 153 197 333 185 

 3545 105 115 148 188 368 185 

 3546 104 116 135 189 343 177 

 3547 99 117 140 212 363 186 

 3548 102 119 150 203 335 182 

 3549 112 114 152 207 369 191 

 3550 159 99 146 173 350 186 

 3551 102 123 148 158 370 180 

MEAN 104 116 143 190 344 178

S.D. 15.6 12.8 9.4 17.3 35.3 16.3

 N 23 23 23 23 22 23

TLL=TOTAL LITTER LOSS 

 EF=Empty Feeder 
11-JAN-2010 17:14

Huntingdon Life Sciences 00-4205 PAGE 321 

 

 APPENDIX 0 P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION -- (GRAMS/KG/DAY) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF LACTATION

ANIMAL# 1 - 4 4 - 7 7 - 14 14 - 21 21 - 28 1 - 28

-----------------------------------------------------------------------------------------------------------------------------------

 4526 92 115 150 186 353 179 

 4527 93 125 151 192 356 183 

 4528 96 109 133 172 322 166 

 4529 79 107 141 168 EF 124 

 4530 PNMD 107 113 146 IW2 398 191 

 4531 143 120 137 167 EF 142 

 4532 113 135 155 253 354 202 

 4533 89 101 125 162 250 146 

 4534 99 114 137 195 364 182 

 4535 92 104 125 180 293 159 

 4536 101 113 148 193 274 166 

 4537 86 98 138 191 355 174 

 4538x HS 119 143 150 137 

 4539 86 105 121 172 271 151 

 4540 82 114 140 192 EF 132 

 4541 121 119 143 195 365 189 

 4542 93 117 143 201 367 184 

 4545 84 100 132 174 324 163 

 4546 82 92 127 165 332 160 

 4547 82 EF 136 178 EF 132 

 4548 111 134 134 166 331 175 

 4549 113 123 146 213 399 199 

 4550 127 122 160 214 356 196 

MEAN 99 113 140 187 337 168 

S.D. 16.8 11.2 10.1 21.6 41.8 22.8

 N 22 21 22 21 18 22 

PNMD=PREGNANT, NO MATING DATE x=EXCLUDED FROM MEAN HS=HUMANE SACRIFICE 

 EF=Empty Feeder IW2=Invalid weight: Weighing error 
11-JAN-2010 17:35

Huntingdon Life Sciences 00-4205 PAGE 322 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 /P E D D D /E M D D /E D D D /E D D D /E D D D 14 1 5 1 4 4.3 

 1527 D /E D D D /E E D D D /E M D D D D /E E D D /E 13 0 7 1 4 4.8 

 1528 D D /E E D D /E M D D /E D D D /E D D D /E D D 14 0 6 1 4 4.0 

 1529 /M D D /E D D D /E D D D /E D D D /E D D D /E E 14 0 6 1 5 3.8 

 1530 /E D D D /E M D D /E M D D /E E D D D /E E M D 11 0 7 3 4 4.3 

 1531 D /P E D D D /P E D D D /E D D D /P E D D D /E 13 3 5 0 4 4.8 

 1532 D D /E D D D /E M D D /E D /E D /E M D D /E D D 13 0 6 2 5 3.2 

 1533 D D /E D D D /E D D D /E D D D /E M D D /E D D 15 0 5 1 4 4.0 

 1534 /E E D D D /E D D D /E E E D /E D D /E E E E E 9 0 12 0 4 4.0 

 1535 D D /E D D D D /E E D D D /E E D D D /E E D D 14 0 7 0 3 5.0 

 1536 /E D D D /E M D D /E E D D D /E E D D /E D D /E 12 0 8 1 5 4.0 

 1537 D D D /E M D D /E M D D D D /E D D D /E M D D 14 0 4 3 3 4.7 

 1538 D D /E E D D D /E E M D D /E D D D /E M D D /E 12 0 7 2 4 4.5 

 1539 D /E D D D /E D D D /E D D D /E D D D /E D D /E 15 0 6 0 5 3.8 

 1540 D /E D D D /E D D D /E D D /E E D D D D /E E D 14 0 7 0 4 4.3 

 1541 D D /E M D D /E D D D /E D D D /E M D D /E D D 14 0 5 2 4 4.0 

 1542 D D /E D D D /E M D D /E D D D /E M D D /E M D 13 0 5 3 4 4.0 

 1543 D D D /E M D D /E D D D /E M D D /E D D D /E D 14 0 5 2 4 4.0 

 1544 /E D D D /E D D /E E D D D /E D D D /E M D D /E 13 0 7 1 5 4.0 

 1545 D D /E D D D /E D D D /E D D D /E M D D /E D D 15 0 5 1 4 4.0 

 1546 /E E M D D D /E M D D /P E D D D D /E D D D /P 12 2 5 2 4 5.0 

 1547 D D /E D D D /E D D D /E D D D /E D D /E E D D 15 0 6 0 4 3.8 

 1548 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0 

 1549 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 1550 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0 

 1551 D /E M D D /P E D D D /P E D D D /P E D D D /P 12 4 4 1 4 4.8 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE
11-JAN-2010 17:35

Huntingdon Life Sciences 00-4205 PAGE 323 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 D D D /E M D D D D /E D D D /E E M D D /E E D 13 0 6 2 3 5.0 

 2527 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0 

 2528 D D D /E E D D D /E D D D D /P E D D D /E D D 15 1 5 0 3 5.0 

 2529 D /E M D D /E D D D /E D D D /E D D D /E D D D 15 0 5 1 4 4.0 

 2530 /E E D D D /E D D D /E D D /E E D D D /E M D D 13 0 7 1 4 4.3 

 2531 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0 

 2532 D D D /E M D D /E E D D D /P E D D D /P E M D 12 2 5 2 3 4.7 

 2533 D D D /E M D D /E D D D /E M D D /E M D D /E M 12 0 5 4 4 4.0 

 2534 D /E M D D /E E D D D /E M D D /E M D D /E M D 11 0 6 4 4 4.3 

 2535 D /E E D D D /E E D D D D /E D D D /E D /E D /E 13 0 8 0 5 3.8 

 2536 /E D D D /E D D D /E M D D D /P E D D D /E D D 14 1 5 1 4 4.5 

 2537 D D /E E D D D /E E D D D D /E D D D /E D D /E 14 0 7 0 4 4.5 

 2538 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 2539 D D /E E D D D /E E D D D /E E D D D /E D D D 14 0 7 0 3 5.0 

 2540 /E D D D /E M D D /E D D D /E D D D /E M D D /E 13 0 6 2 5 4.0 

 2541 /E D D D /E M D D /E D D D /E D D D /E D D D /E 14 0 6 1 5 4.0 

 2542 D D /E D D D /E D D D /E D D D /E D D D /E M D 15 0 5 1 4 4.0 

 2543 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0 

 2544 D D /E M D D /E D D D /E D D D /E D D D /E M D 14 0 5 2 4 4.0 

 2545 D D D /E M D D /E D D D /E E D D D /E M D D /E 13 0 6 2 4 4.3 

 2546 /M D D /E D D D /E D D D /E M D D /E D D D /E D 14 0 5 2 5 3.8 

 2547 D /E E M D D /E D D D /E D D D /E D D D /E M D 13 0 6 2 4 4.3 

 2548 D D D D D /E D D D /E D D D /E D D D /E M D D 16 0 4 1 3 4.0 

 2549 /E D D D /E D D D /E D D D /E M D D /E M D D /E 13 0 6 2 5 4.0 

 2550 D D /E M D D /E D D D /E M D D /E M D D /E D D 13 0 5 3 4 4.0 

 2551 /E D D D D /E D D D /P E D D D /P E M D D /P E 12 3 5 1 4 4.8 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE
11-JAN-2010 17:35

Huntingdon Life Sciences 00-4205 PAGE 324 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 D D D D /E M D D /E E D D D /P D D D /E D D D 15 1 4 1 3 4.3 

 3527 /E D D D /E D D D /E D D D /E D D D /E M D D D 15 0 5 1 4 4.0 

 3528 D D D /E D D D /E D D D /E D D D /E M D D /E E 14 0 6 1 4 4.0 

 3529 D D D /E D D D /E D D D /E M D D /E M D D /E D 14 0 5 2 4 4.0 

 3530 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0 

 3531 D D /E M D D /E D D D /E D D D /E M D D /E D D 14 0 5 2 4 4.0 

 3532 D D D /E D D D /E D D D /E D D D /E M D D /E D 15 0 5 1 4 4.0 

 3533 /E D D D /E M D D /E M D D /E M D D /E M D D /E 11 0 6 4 5 4.0 

 3534 D D D /E M D D /E D D D /E M D D /E D D D /E D 14 0 5 2 4 4.0 

 3535 D /E M D D /E D D /E E D D /E E D D D /E D D D 13 0 7 1 4 4.0 

 3536 D /E M D D /E D D D /E D D D D /E M D D /E D D 14 0 5 2 4 4.3 

 3537 D D /E D D D /E D D D /E D D D /E M D D /E M D 14 0 5 2 4 4.0 

 3538 D /E E D D /P E D D D /P E M D D /E M D D /P E 10 3 6 2 4 4.5 

 3539 /E D D D D /E M D D /E E M D D /P E M D D /P E 10 2 6 3 4 4.8 

 3540 D /E D D D /E D D D /E D D D /E D D D /E D D D 16 0 5 0 4 4.0 

 3541 D D /E D D D /E D D D /E D D D /E M D D /E D D 15 0 5 1 4 4.0 

 3542 /E D D D /E D D D /E D D D /E D D D /E M D D /E 14 0 6 1 5 4.0 

 3543 /E M D D /E D D D /E D D D /E D D D /E M D D /E 13 0 6 2 5 4.0 

 3544 D D D /E E D D D /E E D D D /E E D D D /E E D 13 0 8 0 3 5.0 

 3545 D D D /E E D D D /E E M D D /E E D D D /E D D 13 0 7 1 3 5.0 

 3546 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 3547 /M D D /E D D D D /E M D D /E D D D /E E D D D 14 0 5 2 4 4.0 

 3548 D /E M D D /E D D D /E M D D D /E E D D D /E M 12 0 6 3 4 4.5 

 3549 /E M D D /E M D D /E M D D /E D D D /E E D D /E 11 0 7 3 5 4.0 

 3550 D D D /E M D D /E D D D /E D D /E E D D D /E D 14 0 6 1 4 4.0 

 3551 /E D D D /E D D D /E D D D /E E D D /E M D D /E 13 0 7 1 5 4.0 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE
11-JAN-2010 17:35

Huntingdon Life Sciences 00-4205 PAGE 325 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE DATA 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (PRE-COHABITATION PERIOD) NUMBER OF DAYS

 IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MDE 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 D D /E M D D /E M D D /E M D D /E M D D /E M D 11 0 5 5 4 4.0 

 4527 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 4528 D D /E M D D /E D D D /E M D D /E M D D /E D D 13 0 5 3 4 4.0 

 4529 /P E M D D /E D D D D /E M D D /E M D D D /E D 12 1 5 3 4 4.8 

 4530 D D D D /E M D D /E D D D /E D D D /E D D D /E 15 0 5 1 4 4.0 

 4531 /E E D D D /E D D D /E M D /E E D D D /E M D D 12 0 7 2 4 4.3 

 4532 D D D /E D D D /E M D D /E D D D /E D D D /E M 14 0 5 2 4 4.0 

 4533 D D D /E M D D /E D D D /E D D D /E M D D /E D 14 0 5 2 4 4.0 

 4534 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 4535 D D D /E D D D /E M D D /E D D D /E D D D /E D 15 0 5 1 4 4.0 

 4536 D D /E D D D /E D D D /E M D D /E D D D /E D D 15 0 5 1 4 4.0 

 4537 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0 

 4538 D /E M D D /E D D D /E M D D /E M D D /E D D D 13 0 5 3 4 4.0 

 4539 D D D /E M D D /E D D D /E M D D /E D D D /E D 14 0 5 2 4 4.0 

 4540 D D D D D /M D D D D D D D D D D D /E M D D 18 0 1 2 1a 12.0a 

 4541 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0 

 4542 D D /E M D D /E D D D /E M D D /E M D D /E M D 12 0 5 4 4 4.0 

 4543 D /E D D D /E D D D /E D D D /E M D D /E M D D 14 0 5 2 4 4.0 

 4544 D D D D D D D D D /E D D D /E M P E E E E E 12 1 7 1 1 4.0 

 4545 /E D D D /E D D /E E D D D /E D D D /E D D D /E 14 0 7 0 5 4.0 

 4546 D D D /E M D D /E D D D /E M D D /E M D D /E D 13 0 5 3 4 4.0 

 4547 D D /E M D D /E D D D /E D D D /E D D D /E D D 15 0 5 1 4 4.0 

 4548 D /E D D D /E D D D /E D D D /E D D D /E D D D 16 0 5 0 4 4.0 

 4549 D /E D D D /E D D D /E D D D /E M D D /E D D D 15 0 5 1 4 4.0 

 4550 D D D /E D D D /E D D D /E D D D /E M D D /E M 14 0 5 2 4 4.0 

 4551 D D D /E D D D /E D D D /E D D D /E M D D /E D 15 0 5 1 4 4.0 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

APSEUDOPREGNANT, EXCLUDED FROM CALCULATION.
11-JAN-2010 17:36

Huntingdon Life Sciences 00-4205 PAGE 326 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 1526 E M D D C 

 1527 E C 

 1528 D C 

 1529 D D C 

 1530 D E E C 

 1531 D D D C 

 1532 D C 

 1533 D C 

 1534 S 

 1535 D C 

 1536 S 

 1537 S 

 1538 E D D C 

 1539 E C 

 1540 D D E E E D D D D D D D D D 

 1541 D C 

 1542 D C 

 1543 D D C 

 1544 M D D C 

 1545 D C 

 1546 S 

 1547 D C 

 1548 D C 

 1549 D D D C 

 1550 D C 

 1551 E S 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
11-JAN-2010 17:36

Huntingdon Life Sciences 00-4205 PAGE 327 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 2526 D D C 

 2527 D D C 

 2528 D S 

 2529 S 

 2530 S 

 2531 D D C 

 2532 D P C 

 2533 D D C 

 2534 D S 

 2535 M D D C 

 2536 D D D D D D D C 

 2537 D D C 

 2538 D D D C 

 2539 D D C 

 2540 M D D C 

 2541 M D D S 

 2542 D C 

 2543 D D E D D D D D D D D D D D 

 2544 D E D D D E D D D E D D D E 

 2545 D D D C 

 2546 D E C 

 2547 D S 

 2548 S 

 2549 D D D E D D D E D D D E D D 

 2550 D C 

 2551 D D D C 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
11-JAN-2010 17:36

Huntingdon Life Sciences 00-4205 PAGE 328 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 3526 D E D D D P E D D D D D D D 

 3527 D D D S 

 3528 D D C 

 3529 D D S 

 3530 D E D D D E D D D E D E D D 

 3531 D S 

 3532 D D S 

 3533 M D D C 

 3534 D D C 

 3535 S 

 3536 D S 

 3537 D E D D D S 

 3538 D D D P C 

 3539 D D D P C 

 3540 S 

 3541 D D S 

 3542 D D D C 

 3543 D D D C 

 3544 D D S 

 3545 E S 

 3546 D D D S 

 3547 S 

 3548 D D D C 

 3549 D D P S 

 3550 D D S 

 3551 D D D S 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
11-JAN-2010 17:36

Huntingdon Life Sciences 00-4205 PAGE 329 

 

 APPENDIX P P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL ESTROUS CYCLE AND MATING DATA 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF EVALUATION (COHABITATION PERIOD) 

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

 4526 D S 

 4527 M D D E D D D S 

 4528 D C 

 4529 D D S 

 4530 M D D E D D D D D D D D D D 

 4531 S 

 4532 D D C 

 4533 D D S 

 4534 M D D C 

 4535 D D S 

 4536 D S 

 4537 D D S 

 4538 S 

 4539 D D S 

 4540 P C 

 4541 M D D S 

 4542 D E D D D S 

 4543 E D D D E D D D E M D D E D 

 4544 D E E E E E E E E E E E E E 

 4545 D D D E S 

 4546 D D S 

 4547 D S 

 4548 S 

 4549 E D D D S 

 4550 D D S 

 4551 D D E D D D E D D D E D D D 

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG
11-JAN-2010 17:38

Huntingdon Life Sciences 00-4205 PAGE 330 

 

 APPENDIX Q P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Alternate Date of Date of Date of 

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

 1526 1026 + P 28-JUL-02 2-AUG-02 24-AUG-02

 1527 1027 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1528 1028 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1529 1029 + P 28-JUL-02 31-JUL-02 22-AUG-02

 1530 1030 + P 28-JUL-02 1-AUG-02 23-AUG-02

 1531 1031 + P 28-JUL-02 1-AUG-02 22-AUG-02

 1532 1032 + P 28-JUL-02 30-JUL-02 20-AUG-02

 1533 1033 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1534 1034 + NP 28-JUL-02 29-JUL-02 

 1535 1035 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1536 1036 + P 28-JUL-02 29-JUL-02 20-AUG-02

 1537 1037 + P 28-JUL-02 29-JUL-02 20-AUG-02

 1538 1038 + NP 28-JUL-02 1-AUG-02 

 1539 1039 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1540 1040 - NNMD 28-JUL-02 

 1541 1041 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1542 1042 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1543 1043 + P 28-JUL-02 31-JUL-02 22-AUG-02

 1544 1044 + P 28-JUL-02 1-AUG-02 23-AUG-02

 1545 1045 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1546 1046 + P 28-JUL-02 29-JUL-02 20-AUG-02

 1547 1047 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1548 1048 + NP 28-JUL-02 30-JUL-02 

 1549 1049 + TLL 28-JUL-02 1-AUG-02 23-AUG-02

 1550 1050 + P 28-JUL-02 30-JUL-02 21-AUG-02

 1551 1051 + P 28-JUL-02 30-JUL-02 21-AUG-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS P=PREGNANT 
11-JAN-2010 17:38

Huntingdon Life Sciences 00-4205 PAGE 331 

 

 APPENDIX Q P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Alternate Date of Date of Date of 

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

 2526 2026 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2527 2027 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2528 2028 + P 28-JUL-02 30-JUL-02 21-AUG-02

 2529 2029 + P 28-JUL-02 29-JUL-02 20-AUG-02

 2530 2030 + P 28-JUL-02 29-JUL-02 20-AUG-02

 2531 2031 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2532 2032 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2533 2033 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2534 2034 + P 28-JUL-02 30-JUL-02 21-AUG-02

 2535 2035 + P 28-JUL-02 1-AUG-02 23-AUG-02

 2536 2036 + HS 28-JUL-02 5-AUG-02 

 2537 2037 + P 28-JUL-02 31-JUL-02 23-AUG-02

 2538 2038 + P 28-JUL-02 1-AUG-02 23-AUG-02

 2539 2039 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2540 2040 + P 28-JUL-02 1-AUG-02 23-AUG-02

 2541 2041 + P 28-JUL-02 1-AUG-02 23-AUG-02

 2542 2042 + P 28-JUL-02 30-JUL-02 22-AUG-02

 2543 2043 - PNMD 28-JUL-02 26-AUG-02

 2544 2044 - NNMD 28-JUL-02 

 2545 2045 + P 28-JUL-02 1-AUG-02 23-AUG-02

 2546 2046 + P 28-JUL-02 31-JUL-02 22-AUG-02

 2547 2047 + P 28-JUL-02 30-JUL-02 20-AUG-02

 2548 2048 + P 28-JUL-02 29-JUL-02 20-AUG-02

 2549 2049 - NNMD 28-JUL-02 

 2550 2050 + P 28-JUL-02 30-JUL-02 21-AUG-02

 2551 2051 + P 28-JUL-02 1-AUG-02 23-AUG-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE P=PREGNANT 
11-JAN-2010 17:38

Huntingdon Life Sciences 00-4205 PAGE 332 

 

 APPENDIX Q P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Alternate Date of Date of Date of 

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

 3526 3026 - NNMD 28-JUL-02 

 3527 3027 + P 28-JUL-02 1-AUG-02 23-AUG-02

 3528 3028 + P 28-JUL-02 31-JUL-02 22-AUG-02

 3529 3029 + P 28-JUL-02 31-JUL-02 22-AUG-02

 3530 3030 - NNMD 28-JUL-02 

 3531 3031 + P 28-JUL-02 30-JUL-02 21-AUG-02

 3532 3032 + P 28-JUL-02 31-JUL-02 22-AUG-02

 3533 3033 + P 28-JUL-02 1-AUG-02 22-AUG-02

 3534 3034 + TLL 28-JUL-02 31-JUL-02 23-AUG-02

 3535 3035 + P 28-JUL-02 29-JUL-02 20-AUG-02

 3536 3036 + P 28-JUL-02 30-JUL-02 21-AUG-02

 3537 3037 + P 28-JUL-02 3-AUG-02 25-AUG-02

 3538 3038 + P 28-JUL-02 2-AUG-02 24-AUG-02

 3539 3039 + P 28-JUL-02 2-AUG-02 24-AUG-02

 3540 3040 + P 28-JUL-02 29-JUL-02 20-AUG-02

 3541 3041 + P 28-JUL-02 31-JUL-02 21-AUG-02

 3542 3042 + P 28-JUL-02 1-AUG-02 23-AUG-02

 3543 3035 + P 29-JUL-02 1-AUG-02 23-AUG-02

 3544 3044 + P 28-JUL-02 31-JUL-02 22-AUG-02

 3545 3045 + P 28-JUL-02 30-JUL-02 21-AUG-02

 3546 3046 + P 28-JUL-02 1-AUG-02 23-AUG-02

 3547 3047 + P 28-JUL-02 29-JUL-02 20-AUG-02

 3548 3048 + P 28-JUL-02 1-AUG-02 23-AUG-02

 3549 3049 + P 28-JUL-02 1-AUG-02 23-AUG-02

 3550 3050 + P 28-JUL-02 31-JUL-02 21-AUG-02

 3551 3051 + P 28-JUL-02 1-AUG-02 23-AUG-02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS P=PREGNANT 
11-JAN-2010 17:38

Huntingdon Life Sciences 00-4205 PAGE 333 

 

 APPENDIX Q P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL MATING ASSIGNMENTS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Alternate Date of Date of Date of 

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

 4526 4026 + P 28-JUL-02 30-JUL-02 21-AUG-02

 4527 4027 + P 28-JUL-02 5-AUG-02 27-AUG-02

 4528 4028 + P 28-JUL-02 30-JUL-02 21-AUG-02

 4529 4029 + P 28-JUL-02 31-JUL-02 21-AUG-02

 4530 4030 - PNMD 28-JUL-02 22-AUG-02

 4531 4031 + P 28-JUL-02 29-JUL-02 20-AUG-02

 4532 4032 + P 28-JUL-02 31-JUL-02 23-AUG-02

 4533 4033 + P 28-JUL-02 31-JUL-02 22-AUG-02

 4534 4034 + P 28-JUL-02 1-AUG-02 23-AUG-02

 4535 4035 + P 28-JUL-02 31-JUL-02 22-AUG-02

 4536 4036 + P 28-JUL-02 30-JUL-02 21-AUG-02

 4537 4037 + P 28-JUL-02 31-JUL-02 22-AUG-02

 4538 4038 + HS 28-JUL-02 29-JUL-02 20-AUG-02

 4539 4039 + P 28-JUL-02 31-JUL-02 22-AUG-02

 4540 4040 + P 28-JUL-02 30-JUL-02 21-AUG-02

 4541 4041 + P 28-JUL-02 1-AUG-02 23-AUG-02

 4542 4042 + P 28-JUL-02 3-AUG-02 26-AUG-02

 4543 4043 - NNMD 28-JUL-02 

 4544 4044 - NNMD 28-JUL-02 

 4545 4045 + P 28-JUL-02 2-AUG-02 23-AUG-02

 4546 4046 + P 28-JUL-02 31-JUL-02 22-AUG-02

 4547 4047 + P 28-JUL-02 30-JUL-02 21-AUG-02

 4548 4048 + P 28-JUL-02 29-JUL-02 20-AUG-02

 4549 4049 + P 28-JUL-02 2-AUG-02 24-AUG-02

 4550 4050 + P 28-JUL-02 31-JUL-02 22-AUG-02

 4551 4051 - NP 28-JUL-02 

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive - = Sperm/Plug Negative N = Not Mated

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE P=PREGNANT 
22-JAN-2010 12:28

Huntingdon Life Sciences 00-4205 PAGE 334 

 

 APPENDIX R P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 1526 12 0 12 4 8 4 8 4 6 4 6 4 6 4 6 12 22 

 1527 12 0 12 8 4 8 4 6 4 6 4 6 4 6 4 13 22 

 1528 9 0 9 5 4 5 3 5 3 5 2 5 2 5 2 10 22 

 1529 12 0 12 4 7 4 7 4 6 4 6 4 6 4 6 13 22 

 1530 5 0 5 2 3 2 3 2 3 2 3 2 3 2 3 5 22 

 1531 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 14 21 

 1532 13 0 13 9 4 9 4 6 4 6 4 6 4 6 4 12 21 

 1533 14 1 15 5 9 5 9 5 5 5 5 5 5 5 5 15 22 

 1534x NP 

 1535 11 0 11 5 5 5 5 5 5 4 5 4 5 4 5 12 22 

 1536 12 0 12 5 7 5 7 5 5 5 5 5 5 5 5 12 22 

 1537 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 16 22 

 1538x NP 

 1539 12 1 13 6 6 6 6 5 5 5 5 5 5 5 5 17 22 

 1540xNNMD 

 1541 15 0 15 7 8 7 7 5 5 5 5 5 5 5 5 17 22 

 1542 11 0 11 6 5 6 5 5 5 5 5 5 5 5 5 11 22 

 1543 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 17 22 

 1544 12 1 13 7 5 7 5 5 5 5 5 5 5 5 5 13 22 

 1545 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22 

 1546 13 0 13 4 9 4 9 4 6 4 6 4 6 4 6 15 22 

 1547 6 0 6 4 2 4 2 4 2 4 2 4 2 4 2 6 22 

 1548x NP 

 1549 TLL 13 1 14 7 6 0 0 0 0 0 0 0 0 0 0 14 22 

 1550 12 0 12 8 3 8 3 7 3 7 3 7 3 7 3 12 22 

 1551 9 0 9 3 6 3 6 3 6 3 6 3 6 3 6 9 22 

MEAN 11.9 0.2 12.1 6.0 5.8 6.0 5.7 4.8 4.7 4.7 4.6 4.7 4.6 4.7 4.6 12.7 21.9 

S.D. 2.7 0.4 2.8 2.1 2.0 2.1 2.0 1.0 1.1 1.1 1.2 1.1 1.2 1.1 1.2 3.2 0.3 

 N 22 22 22 22 22 21 21 21 21 21 21 21 21 21 21 22 22 

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY 

 DAY 4 COLUMNS = PRECULLING 
22-JAN-2010 12:28

Huntingdon Life Sciences 00-4205 PAGE 335 

 

 APPENDIX R P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 2526 16 0 16 12 4 12 4 6 4 6 4 6 4 6 4 18 22 

 2527 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 15 22 

 2528 16 0 16 7 9 7 9 5 5 5 5 5 5 5 5 16 22 

 2529 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 15 22 

 2530 13 1 14 6 7 6 7 5 5 5 5 5 5 5 5 14 22 

 2531 16 0 16 7 8 7 8 5 5 5 5 5 5 5 5 17 22 

 2532 13 0 13 9 4 9 4 6 4 6 4 6 4 6 4 15 22 

 2533 17 0 17 9 8 9 8 5 5 5 5 5 5 5 5 16 22 

 2534 14 0 14 9 5 9 5 5 5 5 5 5 5 5 5 14 22 

 2535 10 0 10 7 3 7 3 7 3 7 3 7 3 7 3 10 22 

 2536x HS 18 

 2537 4 0 4 3 1 3 1 3 1 3 1 3 1 3 1 7 23 

 2538 11 0 11 5 4 5 4 5 4 5 4 5 4 5 4 11 22 

 2539 14 0 14 5 9 5 9 5 5 5 5 5 5 5 5 14 22 

 2540 11 0 11 6 5 6 5 5 5 5 5 5 5 5 5 12 22 

 2541 14 0 14 5 9 5 9 5 5 5 5 5 5 5 5 16 22 

 2542 13 0 13 5 7 5 7 5 5 5 5 5 5 5 5 15 22 

 2543 PNMD 16 0 16 7 9 7 9 5 5 5 5 5 5 5 5 18 no day 0 

 2544xNNMD 

 2545 12 0 12 5 7 4 7 4 6 4 6 4 6 4 6 13 22 

 2546 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 16 22 

 2547 12 0 12 4 8 4 8 4 6 4 6 4 6 4 6 12 21 

 2548 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 14 22 

 2549xNNMD 

 2550 11 1 12 3 8 3 8 3 7 3 7 3 7 3 7 15 22 

 2551 12 0 12 4 8 4 8 4 6 4 6 4 6 4 6 12 22 

MEAN 13.0 0.1 13.1 6.5 6.3 6.5 6.3 4.9 4.8 4.9 4.8 4.9 4.8 4.9 4.8 14.1 22.0 

S.D. 2.7 0.3 2.7 2.2 2.2 2.3 2.2 0.9 1.2 0.9 1.2 0.9 1.2 0.9 1.2 2.6 0.3 

 N 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 22 

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN 

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY 

 DAY 4 COLUMNS = PRECULLING 
22-JAN-2010 12:28

Huntingdon Life Sciences 00-4205 PAGE 336 

 

 APPENDIX R P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 3526xNNMD 

 3527 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 16 22 

 3528 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 15 22 

 3529 15 0 15 6 8 6 8 5 5 5 5 5 5 5 5 16 22 

 3530xNNMD 

 3531 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 16 22 

 3532 12 0 12 7 5 7 5 5 5 5 5 5 5 5 5 13 22 

 3533 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 15 21 

 3534 TLL 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 3 23 

 3535 15 0 15 8 6 8 6 5 5 5 5 5 5 5 5 15 22 

 3536 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 14 22 

 3537 13 0 13 11 2 11 2 8 2 8 2 8 2 8 2 14 22 

 3538 12 0 12 7 5 7 5 5 5 5 5 5 4 5 4 14 22 

 3539 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 14 22 

 3540 13 0 13 7 6 7 6 5 5 5 5 5 4 5 4 13 22 

 3541 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 14 21 

 3542 10 0 10 6 4 6 4 6 4 6 4 6 4 6 4 10 22 

 3543 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 16 22 

 3544 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 14 22 

 3545 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 14 22 

 3546 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 16 22 

 3547 10 0 10 6 4 6 4 6 4 6 4 6 4 6 4 14 22 

 3548 11 0 11 5 6 5 6 5 5 5 5 5 5 5 5 17 22 

 3549 14 1 15 6 8 6 8 5 5 5 5 5 5 5 5 15 22 

 3550 16 1 17 8 8 8 8 5 5 5 5 5 5 5 5 17 21 

 3551 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 17 22 

MEAN 13.0 0.1 13.1 6.9 6.5 6.9 6.5 5.2 4.8 5.2 4.8 5.2 4.7 5.2 4.7 14.3 21.9 

S.D. 2.9 0.3 2.9 1.6 2.0 1.6 2.0 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 2.9 0.4 

 N 24 24 24 23 23 23 23 23 23 23 23 23 23 23 23 24 24 

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN 

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY 

 DAY 4 COLUMNS = PRECULLING 
22-JAN-2010 12:28

Huntingdon Life Sciences 00-4205 PAGE 337 

 

 APPENDIX R P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL DELIVERY AND LITTER DATA 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

 DAYS IMPLAN- GESTATION

 LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

 4526 13 0 13 7 6 7 6 5 5 5 5 5 5 5 5 13 22 

 4527 11 1 12 6 5 6 5 5 5 5 5 5 5 5 5 12 22 

 4528 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 16 22 

 4529 15 0 15 11 4 11 4 6 4 6 4 6 4 6 4 16 21 

 4530 PNMD 15 0 15 6 9 6 9 5 5 5 5 5 5 5 5 16 no day 0 

 4531 14 0 14 7 7 7 7 5 5 5 5 5 5 5 5 15 22 

 4532 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 14 23 

 4533 8 1 9 5 3 5 3 5 3 5 3 5 3 5 3 17 22 

 4534 10 2 12 7 3 7 3 7 3 7 3 7 3 7 3 13 22 

 4535 15 1 16 10 5 10 5 5 5 5 5 5 5 5 5 16 22 

 4536 14 0 14 6 8 6 8 5 5 5 5 5 5 5 5 17 22 

 4537 14 0 14 5 9 5 9 5 5 5 5 5 5 5 5 15 22 

 4538x HS 14 1 15 5 9 5 9 5 5 5 5 0 0 0 0 16 22 

 4539 9 0 9 3 5 2 5 2 5 2 5 2 5 2 5 16 22 

 4540 13 0 13 6 7 5 7 5 5 5 5 5 5 5 5 14 22 

 4541 12 0 12 7 5 7 5 5 5 5 5 5 5 5 5 13 22 

 4542 9 0 9 5 4 5 4 5 4 5 4 5 4 5 4 11 23 

 4543xNNMD 

 4544xNNMD 

 4545 11 0 11 7 4 7 4 6 4 6 4 6 4 6 4 13 21 

 4546 12 2 14 5 6 5 6 5 5 5 5 5 5 5 5 15 22 

 4547 15 0 15 10 5 10 5 5 5 5 5 5 5 5 5 16 22 

 4548 14 0 14 6 8 6 8 5 5 5 5 5 5 5 5 15 22 

 4549 12 0 12 6 6 6 6 5 5 5 5 5 5 5 5 12 22 

 4550 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 14 22 

 4551x NP 

MEAN 12.7 0.3 13.0 6.8 5.8 6.7 5.8 5.0 4.7 5.0 4.7 5.0 4.7 5.0 4.7 14.5 22.0 

S.D. 2.2 0.6 2.1 1.9 1.7 2.1 1.7 0.8 0.6 0.8 0.6 0.8 0.6 0.8 0.6 1.7 0.4 

 N 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 21 

 NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 

 x=EXCLUDED FROM MEAN 

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY DAY 4 COLUMNS = PRECULLING 
22-JAN-2010 12:31

Huntingdon Life Sciences 00-4205 PAGE 338 

 

 APPENDIX S F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 1526 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4

 1527 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 1528 MK28 MK28 MK28 MK28 MK28 FK28 FD 2 FD11 FK28

 1529 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FK28 FM 1 FK28

 1530 MK28 MK28 FK28 FK28 FK28

 1531 MK28 MK28 MC 4 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FK28

 1532 MK28 MC 4 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 1533 FS MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4

 1535 MK28 MD 8 MK28 MK28 MK28 FD 1 FK28 FK28 FK28 FK28 FK28

 1536 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FK28

 1537 MC 4 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FC 4 FK28 FK28 FK28 FK28

 1539 MS MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

 1541 MK28 MK28 MK28 MC 4 MC 4 MK28 MK28 FK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FM 2

 1542 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

 1543 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4 FK28

 1544 MS MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 1545 MC 4 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FC 4 FK28 FK28 FK28 FK28 FC 4

 1546 MK28 FK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FC 4

 1547 MK28 MK28 MK28 MK28 FK28 FK28

 1549 FS MD 2 MD 2 MD 2 MD 2 MD 2 MD 2 MD 2 FD 2 FD 2 FD 2 FD 2 FD 2 FD 2

 1550 MC 4 MK28 MM 1 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

 1551 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED M-MISSING K-SCHEDULED SACRIFICE 
22-JAN-2010 12:31

Huntingdon Life Sciences 00-4205 PAGE 339 

 

 APPENDIX S F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 2526 MC 4 MC 4 MK28 MC 4 MK28 MC 4 MK28 MC 4 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28

 2527 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 FC 4 FK28 FK28 FK28 FK28 FK28

 2528 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4 FC 4

 2529 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

 2530 FS MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

 2531 MC 4 MK28 MK28 MK28 MK28 MC 4 MK28 FC 4 FK28 FC 4 FC 4 FK28 FK28 FM 1 FK28 FK28

 2532 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28

 2533 MK28 MC 4 MC 4 MC 4 MK28 MK28 MK28 MC 4 MK28 FC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4

 2534 MK28 MC 4 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 2535 MK28 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

 2537 MK28 MK28 MK28 FK28

 2538 MK28 MK28 MK28 MD 0 MK28 MK28 FK28 FM 1 FK28 FK28 FK28

 2539 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FC 4 FC 4 FK28 FK28 FC 4

 2540 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 2541 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FC 4 FC 4

 2542 MD 0 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FC 4 FK28 FK28

 2543 MC 4 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FC 4 FK28 FK28 FC 4

 2545 MK28 MK28 MK28 MM 2 MK28 FK28 FK28 FK28 FK28 FC 4 FK28 FK28

 2546 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FC 4 FK28

 2547 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FK28

 2548 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

 2550 FS MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28 FK28 FK28

 2551 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED M-MISSING K-SCHEDULED SACRIFICE 
22-JAN-2010 12:31

Huntingdon Life Sciences 00-4205 PAGE 340 

 

 APPENDIX S F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 3527 MC 4 MC 4 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

 3528 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FC 4 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

 3529 MM 1 MC 4 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FC 4 FK28 FK28 FK28 FK28 FC 4

 3531 MC 4 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

 3532 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 MK28

 3533 MK28 MC 4 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4

 3534 FD 1 FD 1

 3535 MK28 MD 1 MK28 MK28 MC 4 MK28 MC 4 MK28 FK28 FK28 FK28 MC 4 FC 4 FK28 FK28

 3536 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 3537 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MK28 MK28 MC 4 MK28 FK28 FK28

 3538 MK28 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FM17 FK28 FK28 FK28

 3539 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

 3540 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28 FC 4 FD16 FK28

 3541 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MC 4 FK28 FC 4 FK28 FK28 FK28 FK28

 3542 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 3543 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FC 4 FK28 FC 4 FK28 FC 4

 3544 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FK28

 3545 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28

 3546 MC 4 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

 3547 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 3548 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FK28

 3549 FS MK28 MK28 MK28 MK28 MC 4 MK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4

 3550 FS MC 4 MK28 MK28 MC 4 MC 4 MK28 MK28 MK28 FK28 FC 4 FC 4 FK28 FC 4 FK28 FK28 FK28

 3551 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FC 4

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED M-MISSING K-SCHEDULED SACRIFICE 
22-JAN-2010 12:31

Huntingdon Life Sciences 00-4205 PAGE 341 

 

 APPENDIX S F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

 4526 MK28 MC 4 MK28 MK28 MC 4 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28

 4527 FS MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 4528 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 MC 4 MK28 FK28 FK28 FC 4 FK28 FK28 FK28

 4529 MK28 MK28 MK28 MC 4 MK28 MK28 MK28 MC 4 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28

 4530 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FC 4

 4531 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4

 4532 MC 4 MC 4 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

 4533 MS MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

 4534 MS FS MK28 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

 4535 MS MK28 MK28 MK28 MC 4 MK28 MK28 MC 4 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

 4536 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FC 4

 4537 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FC 4 FC 4 FC 4 FK28

 4538 US MU14 MU14 MU14 MU14 MU14 FU14 FU14 FU14 FU14 FU14 FC 4 FC 4 FC 4 FC 4

 4539 UM 0 MM 2 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 4540 MK28 MK28 MK28 MM 3 MK28 MK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

 4541 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

 4542 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 4545 MK28 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

 4546 FS FD 0 FS MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

 4547 MC 4 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

 4548 MK28 MC 4 MK28 MK28 MK28 MK28 FC 4 FK28 FC 4 FC 4 FK28 FK28 FK28 FK28

 4549 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

 4550 MC 4 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M-MALE F-FEMALE U-UNCERTAIN

PUP STATUS CODES: A-ALIVE S-STILLBORN D-DIED C-CULLED M-MISSING K-SCHEDULED SACRIFICE U-UNSCHEDULED SACRIFICE 
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 342 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1526 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 1527 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 1528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 343 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1528 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 SWOLLEN PAW(S) P 

 PUP# 8 SCABS P 

 1529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 1530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 1531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 344 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1531 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1532 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1533 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 345 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 1536 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 1537 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 346 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1537 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 1539 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 347 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1541 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P 

 1542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 1543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 348 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1544 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 1545 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 1546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 349 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1546 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 1547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 1549 PUP# 2 WITHIN NORMAL LIMITS P 

 PUP# 3 WITHIN NORMAL LIMITS P 

 PUP# 4 WITHIN NORMAL LIMITS P 

 PUP# 5 WITHIN NORMAL LIMITS P 

 PUP# 6 WITHIN NORMAL LIMITS P 

 PUP# 7 WITHIN NORMAL LIMITS P 

 PUP# 8 WITHIN NORMAL LIMITS P 

 PUP# 9 WITHIN NORMAL LIMITS P 

 PUP# 10 WITHIN NORMAL LIMITS P 

 PUP# 11 WITHIN NORMAL LIMITS P 

 PUP# 12 WITHIN NORMAL LIMITS P 

 PUP# 13 WITHIN NORMAL LIMITS P 

 PUP# 14 WITHIN NORMAL LIMITS P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:43

Huntingdon Life Sciences 00-4205 PAGE 350 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 1550 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 1551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:45

Huntingdon Life Sciences 00-4205 PAGE 351 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2526 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 SWOLLEN SNOUT P 

 PUP# 9 ULCERATION - CERVICAL P 

 PUP# 9 SCABS P 

 PUP# 9 OPACITY - UNILATERAL 3 P

 2527 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:45

Huntingdon Life Sciences 00-4205 PAGE 352 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2527 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 2528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 PUP# 16 WITHIN NORMAL LIMITS P P 

 2529 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 353 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2530 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 2531 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 354 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2532 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 2533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P 

 PUP# 17 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 355 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 2535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 2537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 356 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2538 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 2539 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 2540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 357 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2540 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 2541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 2542 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 358 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2543 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P 

 2545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 359 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 2547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 2548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:44

Huntingdon Life Sciences 00-4205 PAGE 360 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 2548 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 2550 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 2551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 361 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3527 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P 

 3528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 362 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3529 PUP# 1 WITHIN NORMAL LIMITS P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 3531 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P 

 PUP# 5 DOME HEAD P P

 PUP# 7 DOME HEAD P P

 PUP# 11 DOME HEAD P P

 PUP# 13 DOME HEAD P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 363 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3532 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 3533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

 3534 PUP# 1 WITHIN NORMAL LIMITS P 

 PUP# 2 WITHIN NORMAL LIMITS P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 364 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P 

 3536 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 365 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3538 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 3539 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 366 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3539 PUP# 7 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 367 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3541 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 3542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 3543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 368 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3544 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 3546 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 369 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3546 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 3547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 3548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 370 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3549 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 3550 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P

 PUP# 17 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:47

Huntingdon Life Sciences 00-4205 PAGE 371 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 3551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:50

Huntingdon Life Sciences 00-4205 PAGE 372 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4526 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 4527 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 4528 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 373 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4528 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 4529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 4530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 374 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4530 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P 

 4531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 4532 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 375 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4532 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 4533 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 4534 PUP# 3 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 4535 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 376 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4535 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 PUP# 16 WITHIN NORMAL LIMITS P P P P P P 

 4536 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 4537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 377 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4537 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 4538 PUP# 2 WITHIN NORMAL LIMITS P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P 

 PUP# 7 WITHIN NORMAL LIMITS P P P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P 

 PUP# 9 WITHIN NORMAL LIMITS P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P 

 PUP# 12 WITHIN NORMAL LIMITS P P 

 PUP# 13 WITHIN NORMAL LIMITS P P 

 PUP# 14 WITHIN NORMAL LIMITS P P 

 PUP# 15 WITHIN NORMAL LIMITS P P 

 4539 PUP# 2 WITHIN NORMAL LIMITS P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 4540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P 

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 378 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4540 PUP# 5 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P 

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 4541 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P 

 4542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 379 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P 

 4546 PUP# 4 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P 

 4547 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P 

 PUP# 6 WITHIN NORMAL LIMITS P P 

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 380 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4547 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

 4548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P 

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 4549 PUP# 1 WITHIN NORMAL LIMITS P P P P P P 

 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

 PUP# 4 WITHIN NORMAL LIMITS P P 

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P P P P P

 PUP# 10 WITHIN NORMAL LIMITS P P P P P P

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:49

Huntingdon Life Sciences 00-4205 PAGE 381 

 

 APPENDIX T F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

 4549 PUP# 12 WITHIN NORMAL LIMITS P P 

 4550 PUP# 1 WITHIN NORMAL LIMITS P P 

 PUP# 2 WITHIN NORMAL LIMITS P P 

 PUP# 3 WITHIN NORMAL LIMITS P P 

 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

 PUP# 8 WITHIN NORMAL LIMITS P P P P P P

 PUP# 9 WITHIN NORMAL LIMITS P P 

 PUP# 10 WITHIN NORMAL LIMITS P P 

 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

 PUP# 14 WITHIN NORMAL LIMITS P P P P P P

 PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1-SLIGHT 2-MODERATE 3-MARKED P-PRESENT
11-JAN-2010 17:54

Huntingdon Life Sciences 00-4205 PAGE 382 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 8.3 8.2 8.7 8.6 8.9 7.6 7.8 8.4 8.6 8.5 8.4 8.0 7.7 

 1527 8.0 8.6 8.0 7.9 8.1 7.5 7.4 8.6 8.3 8.4 7.3 7.7 8.3 

 1528 7.4 8.3 7.6 7.9 6.0 8.5 8.3 5.3 7.1 7.8 

 1529 7.6 7.7 7.5 8.0 8.1 7.1 7.7 7.4 7.2 7.4 7.1 M 7.9 

 1530 8.5 8.2 8.9 8.4 8.2 8.7 

 1531 5.9 6.2 6.2 6.3 6.3 6.2 5.8 6.1 6.0 5.5 5.9 5.3 5.5 5.6 

 1532 6.4 6.9 6.2 5.8 6.6 6.5 6.9 6.9 6.8 6.3 6.3 6.0 5.8 6.0 

 1533 7.5 S 7.4 7.7 7.6 7.7 8.3 7.6 6.7 7.3 7.0 7.7 7.7 7.3 7.6 7.0 

 1534 NOT PREGNANT 

 1535 8.3 8.4 8.8 8.1 8.0 8.5 D 8.7 7.8 8.4 8.3 8.2 

 1536 7.9 8.8 7.9 8.1 8.3 8.3 7.8 8.0 7.9 6.9 6.8 7.1 8.3 

 1537 7.0 7.1 7.0 7.9 6.7 7.0 6.9 7.3 7.2 6.3 7.1 6.6 7.0 6.5 7.0 7.3 6.8 

 1538 NOT PREGNANT 

 1539 7.5 S 8.3 7.2 7.3 8.1 7.6 7.9 7.1 6.9 8.0 7.1 7.6 6.9 

 1540 NOT PREG., NO MATING DATE 

 1541 7.3 7.2 8.0 7.1 7.4 7.3 7.9 6.8 6.5 6.7 7.3 6.9 7.8 7.0 7.9 7.4 

 1542 7.5 8.2 7.7 7.8 7.7 7.7 7.5 7.8 6.7 7.2 7.1 7.2 

 1543 7.3 7.7 7.7 7.7 7.5 8.0 7.7 7.4 7.7 7.1 7.3 6.9 6.9 6.8 6.8 5.8 

 1544 7.5 S 8.4 7.9 8.1 7.7 7.4 7.7 7.1 7.6 7.3 6.9 7.5 7.0 

 1545 6.5 7.0 6.5 7.0 5.9 6.3 6.6 7.4 6.4 6.4 6.8 6.9 6.2 6.4 5.4 5.6 

 1546 7.6 8.2 7.8 8.0 8.1 8.1 7.9 7.4 7.7 6.7 7.0 7.7 7.2 7.1 

 1547 8.4 8.6 8.8 8.5 8.0 8.5 7.9 

 1548 NOT PREGNANT 

 1549 5.2 S 5.2 5.3 5.6 4.8 5.3 5.6 6.1 5.0 5.1 4.7 4.8 4.9 4.8 

 1550 7.7 8.0 8.2 M 7.7 7.9 7.8 8.1 8.4 8.3 6.7 7.0 6.4 

 1551 7.4 8.0 7.6 7.7 7.1 7.0 7.1 7.6 7.4 7.0 

MEAN 7.4

S.D. 0.81

N 22

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:54

Huntingdon Life Sciences 00-4205 PAGE 383 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 6.9 7.2 7.3 6.4 6.5 7.5 7.1 6.7 7.0 7.7 7.1 6.6 7.2 6.3 6.4 6.3 6.5 

 2527 6.3 6.8 6.5 5.6 7.0 7.0 6.9 6.5 6.6 6.2 6.2 5.4 6.1 5.6 5.7 

 2528 7.2 7.7 7.6 6.9 7.8 7.1 7.1 7.4 7.2 7.4 6.5 7.9 7.0 7.2 6.8 6.5 6.7 

 2529 7.4 7.9 7.6 7.5 7.5 7.5 7.6 7.4 7.9 7.1 5.9 7.4 7.4 7.1 

 2530 7.5 S 7.7 7.4 7.6 8.2 8.2 7.7 7.3 7.6 7.2 7.3 7.4 7.5 7.0 

 2531 6.8 7.0 7.0 7.2 6.8 6.8 6.7 7.1 7.0 6.7 6.9 6.7 6.3 6.4 M 6.9 6.2 

 2532 7.3 7.4 7.7 7.7 6.7 7.6 7.5 7.3 7.1 7.4 7.2 7.0 7.2 7.2 

 2533 7.0 7.0 7.8 7.9 7.1 6.9 7.4 6.9 6.3 6.9 7.4 6.8 6.7 6.0 6.9 6.5 7.2 7.1 

 2534 7.4 6.8 6.5 7.0 8.1 7.3 8.0 8.2 7.2 8.4 6.5 6.7 7.8 7.5 7.3 

 2535 7.4 7.0 6.9 7.3 7.9 8.2 7.7 7.3 7.9 7.4 6.8 

 2536 HUMANE SACRIFICE 

 2537 8.2 7.5 7.8 8.9 8.5 

 2538 8.1 8.2 8.5 8.2 D 8.7 8.0 7.4 M 7.8 8.2 8.2 

 2539 7.0 8.0 7.6 7.2 7.5 7.2 7.2 6.9 7.3 7.0 6.7 6.5 6.0 6.2 6.4 

 2540 7.7 7.3 7.8 8.0 8.2 7.4 7.7 8.2 7.7 7.2 7.5 7.4 

 2541 7.5 7.1 8.4 7.8 8.2 7.0 6.9 7.0 8.2 7.4 8.0 7.1 7.3 7.6 7.4 

 2542 7.2 D 7.1 7.7 7.6 7.0 8.0 6.9 6.5 6.9 7.1 7.6 6.4 7.2 

 2543 6.8 7.1 7.2 7.3 7.2 7.2 5.7 7.0 7.1 6.5 6.0 6.6 7.0 7.2 6.8 6.5 7.0 

 2544 NOT PREG., NO MATING DATE 

 2545 6.7 6.9 7.0 7.1 5.7 6.9 6.3 7.0 7.2 7.1 6.1 7.3 6.2 

 2546 7.4 7.5 7.6 7.6 5.9 7.5 7.3 8.0 7.9 7.6 7.5 7.4 7.0 7.5 7.2 

 2547 6.6 7.1 6.8 7.2 6.4 6.1 6.6 6.4 6.6 6.5 6.5 6.3 6.6 

 2548 7.0 7.0 7.2 7.3 7.3 7.9 7.4 6.9 7.4 7.4 6.6 7.2 4.9 6.5 

 2549 NOT PREG., NO MATING DATE 

 2550 6.8 S 5.0 9.1 5.4 6.4 7.7 7.0 6.5 6.2 8.0 7.8 6.1 

 2551 7.6 7.6 7.5 7.6 8.0 8.0 7.8 7.3 7.7 7.7 7.5 7.1 7.3 

MEAN 7.2

S.D. 0.46

N 23

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:54

Huntingdon Life Sciences 00-4205 PAGE 384 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREG., NO MATING DATE 

 3527 6.7 6.7 7.1 6.7 6.9 6.3 6.6 7.4 7.0 7.3 7.1 6.2 6.1 6.5 6.5 6.7 6.5 

 3528 7.4 7.2 7.1 7.6 8.0 8.1 7.5 6.9 6.6 7.1 7.3 6.6 7.7 7.7 7.1 7.9 

 3529 6.9 M 7.1 7.3 6.7 7.1 6.8 7.3 7.0 6.9 7.2 6.8 7.1 6.8 5.4 6.4 

 3530 NOT PREG., NO MATING DATE 

 3531 6.8 7.1 6.7 6.5 7.2 7.2 7.3 6.6 7.4 6.0 6.9 6.0 6.7 7.1 

 3532 7.6 7.5 7.5 7.9 7.9 6.7 8.0 7.7 6.8 8.1 7.6 7.2 7.8 

 3533 6.1 6.3 6.1 5.8 6.2 6.2 6.6 6.1 6.1 6.1 6.1 5.8 5.9 5.5 6.0 6.5 

 3534 TOTAL LITTER LOSS 

 3535 6.3 6.8 D 6.3 7.1 7.1 6.9 7.0 6.4 5.9 6.5 6.0 5.2 6.1 5.8 5.7 

 3536 6.8 5.7 7.4 7.3 7.1 7.1 7.2 6.9 6.5 7.2 6.3 6.5 6.5 6.4 

 3537 7.1 7.4 7.1 7.1 6.9 7.3 7.6 6.9 7.4 7.1 7.0 7.0 6.3 6.9 

 3538 7.3 7.4 7.3 7.0 7.3 7.3 7.2 8.3 6.7 7.0 7.2 7.8 6.9 

 3539 6.7 7.1 6.7 6.6 7.0 6.8 7.0 6.5 6.5 6.4 6.4 6.5 6.9 6.8 

 3540 7.6 7.2 7.6 7.7 7.7 7.9 8.0 7.9 7.4 7.6 7.8 7.4 7.5 7.3 

 3541 7.3 7.3 7.5 8.3 6.1 7.9 8.3 7.6 8.1 4.0 7.6 6.8 7.5 7.6 7.6 

 3542 8.1 8.4 8.3 9.0 8.1 8.5 7.9 7.9 8.4 7.4 6.8 

 3543 6.5 7.2 7.0 6.4 7.1 5.6 4.5 6.8 6.7 6.6 6.9 6.0 6.5 5.7 6.6 7.2 

 3544 6.0 6.1 5.9 6.1 6.2 6.4 5.9 6.5 6.0 5.7 6.1 6.0 5.1 5.7 

 3545 7.3 7.3 8.1 7.6 7.4 7.9 6.6 6.9 7.3 6.4 8.3 7.3 7.3 7.0 

 3546 7.2 7.0 6.8 7.7 7.2 7.4 7.7 7.6 7.3 6.7 6.9 6.6 7.9 7.0 7.1 

 3547 7.1 8.0 7.5 6.8 7.3 7.2 7.5 7.1 6.7 6.7 6.4 

 3548 8.0 8.6 8.3 8.1 8.7 8.0 8.2 7.8 7.6 7.7 8.0 7.5 

 3549 7.9 S 8.5 8.1 8.3 8.1 7.5 8.0 6.8 7.5 7.9 8.1 8.5 7.7 7.5 7.8 

 3550 5.8 S 6.0 6.4 5.9 6.5 5.3 6.0 5.9 5.9 5.2 5.4 5.9 5.7 5.0 5.9 5.6 5.7 

 3551 7.5 7.3 7.8 7.8 7.9 7.9 8.0 7.3 7.5 7.7 7.2 7.1 7.3 7.5 7.2 7.3 

MEAN 7.0

S.D. 0.63

N 23

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:54

Huntingdon Life Sciences 00-4205 PAGE 385 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 1 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 7.4 7.3 8.0 7.8 7.8 7.2 7.4 7.6 7.4 7.0 6.9 7.2 7.2 7.8 

 4527 7.8 S 8.1 7.6 8.3 7.7 7.4 8.2 7.4 7.7 7.7 7.8 8.0 

 4528 6.6 7.0 6.5 6.3 7.2 6.9 7.0 6.8 6.9 6.6 6.3 6.5 6.4 6.5 5.3 6.1 

 4529 6.5 6.3 6.5 6.8 7.2 6.0 6.3 6.4 6.4 6.5 6.8 6.8 6.2 6.9 6.4 6.7 

 4530 6.1 6.2 5.9 5.9 6.7 6.0 6.3 6.0 5.9 6.1 6.3 5.9 6.0 6.9 5.9 6.0 

 4531 7.0 7.3 7.0 6.9 7.6 6.9 7.9 7.4 7.0 6.7 6.4 6.6 7.5 6.8 6.0 

 4532 7.5 7.6 7.1 8.1 7.3 7.8 8.6 8.8 8.1 6.8 7.7 6.5 6.5 7.5 6.8 

 4533 6.9 S 6.7 7.4 7.3 7.5 5.4 6.7 6.5 7.3 

 4534 7.9 S S 7.7 8.1 8.1 8.6 7.6 7.9 8.3 7.4 6.9 8.1 

 4535 7.3 S 7.5 7.5 7.2 7.2 7.5 6.5 8.0 8.1 7.6 7.3 7.2 6.7 7.0 7.1 7.4 

 4536 7.5 6.9 7.4 10.5 7.3 7.4 7.7 6.9 7.3 6.7 7.5 8.0 7.4 7.0 6.7 

 4537 7.7 8.0 8.0 7.6 8.4 8.2 6.9 7.4 7.5 7.7 7.9 7.2 7.5 8.4 7.7 

 4538 7.3 S 7.3 7.4 7.5 7.9 7.2 7.2 6.9 7.6 6.5 7.0 6.3 7.3 8.1 7.4 

 4539 7.5 M 4.0 7.3 9.4 7.9 7.0 8.7 8.0 7.4 

 4540 7.5 7.9 7.7 7.7 7.7 7.7 7.2 7.4 8.2 7.2 7.6 7.3 7.0 7.3 

 4541 7.4 7.2 7.3 7.2 7.5 7.9 7.6 7.8 6.8 7.1 7.3 7.2 7.5 

 4542 8.2 8.7 8.6 7.7 8.6 7.6 8.0 8.3 8.2 8.2 

 4543 NOT PREG., NO MATING DATE 

 4544 NOT PREG., NO MATING DATE 

 4545 7.6 7.7 7.8 8.2 7.9 7.9 7.9 7.8 7.5 6.4 7.4 6.7 

 4546 7.2 S D S 7.1 8.2 7.5 7.4 7.7 6.9 6.9 6.7 6.9 7.6 6.4 

 4547 7.3 7.6 7.5 7.8 7.6 7.2 8.3 7.3 7.0 7.6 7.4 7.2 6.7 7.1 6.3 6.6 

 4548 6.8 6.7 6.7 6.4 7.0 7.0 7.2 6.7 7.0 6.7 6.7 6.6 6.5 6.5 7.2 

 4549 7.2 7.4 7.8 6.6 7.4 7.4 7.1 7.5 6.8 6.7 7.2 7.1 7.2 

 4550 6.8 6.9 7.4 7.0 6.7 7.1 6.6 6.5 7.2 6.9 7.2 6.6 6.8 6.3 6.8 6.5 

 4551 NOT PREGNANT 

MEAN 7.3

S.D. 0.49

N 22

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:55

Huntingdon Life Sciences 00-4205 PAGE 386 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 11.1 11.3 11.6 11.2 11.9 10.0 10.4 11.6 11.5 11.3 10.9 10.6 10.3 

 1527 12.1 12.6 12.3 12.1 12.0 11.8 11.2 12.5 12.7 12.3 10.9 12.0 12.4 

 1528 11.8 13.0 12.5 12.8 8.0 13.5 13.3 D 8.8 12.4 

 1529 10.9 11.0 10.7 11.7 11.2 10.3 11.1 10.8 10.7 11.3 10.7 M 10.6 

 1530 14.1 14.3 14.7 14.3 13.7 13.5 

 1531 8.2 8.6 8.2 8.6 8.9 8.6 8.4 8.4 8.4 8.1 7.9 6.9 7.9 7.9 

 1532 9.6 10.2 9.6 9.0 9.6 10.1 10.2 10.6 9.5 9.4 9.0 9.4 8.9 9.2 

 1533 11.6 S 12.1 11.3 12.3 11.7 12.4 11.6 10.7 11.7 10.8 11.7 11.3 11.7 11.5 10.9 

 1534 NOT PREGNANT 

 1535 12.0 11.7 12.7 11.5 11.8 12.6 D 12.2 10.9 12.4 12.1 12.4 

 1536 13.0 14.1 13.1 13.2 13.5 13.6 13.0 13.1 12.8 12.4 11.4 12.1 13.2 

 1537 10.3 10.7 11.0 11.2 9.7 11.2 10.0 10.3 10.4 10.0 10.9 9.6 10.3 10.3 9.3 9.9 9.9 

 1538 NOT PREGNANT 

 1539 12.2 S 13.5 12.2 12.0 12.6 12.1 13.0 12.0 11.4 12.0 11.4 12.3 11.4 

 1540 NOT PREG., NO MATING DATE 

 1541 11.4 12.0 12.5 12.0 11.5 12.2 12.0 10.8 11.0 10.3 11.9 9.9 11.1 11.7 11.0 M 

 1542 11.3 11.9 12.2 11.6 11.6 11.4 10.7 11.4 10.3 11.0 11.4 10.6 

 1543 10.1 10.3 10.7 11.2 10.6 11.0 10.0 10.4 10.6 9.5 10.6 9.8 9.7 9.4 9.6 8.5 

 1544 11.3 S 12.2 11.7 11.9 11.9 11.5 11.0 10.7 11.0 11.2 10.1 11.1 11.3 

 1545 9.9 10.3 10.1 10.8 9.9 9.8 10.3 11.0 9.5 9.6 9.9 10.5 10.0 9.9 8.8 8.8 

 1546 11.0 12.1 10.9 10.9 11.2 12.0 11.0 10.4 11.5 10.2 10.9 11.0 10.8 10.0 

 1547 14.2 14.0 14.3 14.9 13.4 14.4 14.0 

 1548 NOT PREGNANT 

 1549 TOTAL LITTER LOSS 

 1550 11.8 12.0 12.8 M 12.5 12.5 11.8 12.9 12.2 11.9 11.1 11.0 9.5 

 1551 11.3 12.0 11.8 12.2 11.0 11.1 11.0 11.5 11.0 10.5 

MEAN 11.4

S.D. 1.38

N 21

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:55

Huntingdon Life Sciences 00-4205 PAGE 387 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 9.4 9.4 10.4 9.1 9.9 9.4 9.7 8.9 8.7 10.4 9.9 9.6 9.9 9.1 8.0 9.1 8.5 

 2527 9.4 10.5 10.2 8.5 10.2 10.7 9.3 9.5 8.8 9.3 9.4 7.6 9.6 8.4 9.0 

 2528 8.9 8.8 8.6 8.3 9.8 9.7 9.7 8.2 9.5 9.2 7.6 9.6 8.2 8.9 8.6 9.0 8.1 

 2529 10.3 10.7 10.3 10.5 10.5 10.7 10.7 10.3 10.9 9.8 8.5 10.5 9.9 10.1 

 2530 10.8 S 11.7 10.8 10.9 11.1 11.5 10.6 10.3 10.7 10.6 10.3 11.1 10.1 10.1 

 2531 9.7 10.2 10.0 10.1 9.6 9.4 9.4 10.1 9.2 9.4 9.4 10.0 9.7 9.3 M 10.7 9.5 

 2532 9.5 9.3 9.5 8.9 9.0 9.8 9.8 9.1 9.5 9.8 9.5 9.9 9.6 9.3 

 2533 9.6 9.6 11.2 9.9 9.5 9.6 9.0 10.1 9.1 9.5 10.5 9.8 10.3 8.3 9.6 9.1 9.0 9.7 

 2534 10.0 9.5 9.5 9.2 11.3 10.2 10.0 11.0 10.2 11.2 9.4 9.3 10.3 10.0 9.5 

 2535 12.1 11.5 11.1 11.8 12.6 12.9 12.3 12.0 12.6 12.6 11.3 

 2536 HUMANE SACRIFICE 

 2537 14.0 11.7 14.4 14.9 15.0 

 2538 12.6 12.6 12.9 13.1 D 13.2 12.6 11.5 M 12.2 12.4 12.6 

 2539 9.9 9.6 10.6 10.1 10.3 11.0 10.3 9.8 10.9 10.0 8.7 9.8 9.1 9.0 9.5 

 2540 11.8 11.3 11.9 12.1 12.4 12.0 11.7 12.5 11.9 11.1 11.5 11.4 

 2541 10.2 9.9 11.1 10.3 10.3 9.6 9.4 9.8 10.4 10.4 10.4 10.5 9.6 10.3 10.2 

 2542 10.2 D 10.8 11.2 10.7 9.7 11.1 10.0 9.4 9.9 9.8 10.7 9.4 10.2 

 2543 9.7 10.1 10.6 10.6 10.2 10.3 8.4 9.8 9.9 9.2 8.7 9.3 9.9 9.9 9.4 9.4 9.4 

 2544 NOT PREG., NO MATING DATE 

 2545 10.3 10.6 10.5 10.5 M 10.9 9.5 10.4 10.9 10.1 9.3 11.1 9.2 

 2546 10.5 11.0 10.8 9.8 7.2 10.6 11.0 12.6 10.3 10.4 11.4 10.3 10.0 10.4 10.5 

 2547 9.9 10.5 10.0 10.8 9.5 9.7 10.0 9.8 9.6 9.7 10.2 9.4 10.1 

 2548 10.6 10.3 11.2 11.4 10.9 10.9 11.4 10.6 11.1 10.8 10.5 11.0 7.6 9.7 

 2549 NOT PREG., NO MATING DATE 

 2550 9.9 S 6.2 12.4 7.6 9.6 11.3 10.7 9.9 9.3 10.9 11.6 9.1 

 2551 11.0 11.4 11.0 11.0 11.4 11.6 11.1 11.3 10.9 11.2 10.8 10.4 10.4 

MEAN 10.4

S.D. 1.18

N 23

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:55

Huntingdon Life Sciences 00-4205 PAGE 388 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREG., NO MATING DATE 

 3527 9.7 9.3 10.1 9.7 10.3 9.6 10.1 10.1 9.5 10.4 10.9 8.6 8.9 9.2 10.3 9.7 8.8 

 3528 10.9 10.7 10.4 11.9 11.5 11.8 11.4 10.2 10.2 10.6 10.9 9.7 10.9 11.0 11.2 11.6 

 3529 10.3 M 10.5 9.9 10.4 10.1 11.3 11.1 11.1 9.8 11.2 10.2 10.0 10.2 8.8 10.0 

 3530 NOT PREG., NO MATING DATE 

 3531 9.8 10.4 10.1 9.8 11.0 8.5 10.6 9.3 10.6 8.8 10.2 9.1 9.9 9.7 

 3532 11.4 11.6 11.3 12.2 11.8 9.9 11.8 11.5 10.3 11.8 11.1 11.7 11.5 

 3533 8.8 9.2 8.8 9.1 9.3 8.7 9.5 8.8 9.0 8.6 9.0 8.0 8.0 8.1 8.2 9.3 

 3534 TOTAL LITTER LOSS 

 3535 9.4 9.8 D 9.8 9.8 10.4 11.1 9.8 9.8 8.5 9.6 8.7 7.9 9.1 9.0 8.5 

 3536 9.5 8.4 9.7 10.4 10.0 9.9 9.8 9.9 9.5 9.1 8.9 9.0 9.7 9.7 

 3537 10.8 11.5 10.8 11.0 11.0 11.5 10.7 10.5 11.1 11.1 10.1 10.9 10.4 10.4 

 3538 11.5 11.7 11.8 11.0 12.0 11.0 11.7 12.4 10.4 11.4 11.6 12.2 10.8 

 3539 9.7 10.1 9.8 8.5 10.1 10.0 10.8 9.8 9.4 9.2 9.6 9.7 10.1 8.4 

 3540 11.2 10.7 12.1 12.0 11.7 11.6 11.3 11.5 10.7 10.9 11.2 10.7 10.0 10.8 

 3541 11.4 11.4 11.3 12.4 10.2 12.6 13.0 12.8 11.9 6.7 11.7 10.7 11.2 11.4 11.9 

 3542 12.7 13.0 13.1 13.5 12.8 13.1 12.5 12.7 12.8 12.2 11.4 

 3543 9.0 9.9 10.3 9.2 10.5 7.7 5.9 9.3 9.0 8.5 9.3 8.3 9.2 8.6 8.6 10.1 

 3544 8.3 8.4 8.3 8.3 8.7 8.9 8.8 8.9 7.4 8.0 8.7 8.3 7.3 8.2 

 3545 10.4 11.3 10.9 10.7 11.0 11.3 9.2 9.8 11.0 9.0 10.3 10.5 10.4 10.0 

 3546 10.2 10.1 9.7 11.1 10.1 10.7 10.3 10.9 10.3 9.7 9.8 9.5 10.8 10.2 9.9 

 3547 10.6 11.8 11.0 10.1 10.8 10.8 11.1 10.5 10.2 10.3 9.6 

 3548 11.9 12.6 12.6 11.7 13.0 11.7 11.2 11.8 11.5 11.3 11.8 11.3 

 3549 10.2 S 10.3 9.3 10.5 10.9 10.0 10.5 9.1 9.7 10.8 10.5 10.7 9.3 10.5 10.4 

 3550 7.9 S 7.9 8.1 8.5 8.6 7.7 8.4 7.7 8.0 7.3 7.3 8.0 8.1 7.3 8.0 8.0 7.1 

 3551 10.1 10.3 10.4 11.0 10.4 10.3 9.7 10.1 9.6 10.7 9.8 9.8 10.5 9.2 10.2 9.3 

MEAN 10.2

S.D. 1.16

N 23

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:55

Huntingdon Life Sciences 00-4205 PAGE 389 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 4 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 10.1 9.9 10.7 9.9 10.6 9.7 10.4 10.9 10.0 9.0 9.8 10.0 10.1 10.4 

 4527 10.8 S 11.2 11.3 11.4 10.5 10.9 11.0 10.0 10.3 10.7 10.3 11.0 

 4528 9.5 10.3 9.4 9.3 9.9 9.7 10.2 10.2 9.7 9.7 9.0 9.5 9.2 9.9 7.6 9.4 

 4529 9.2 8.8 9.8 9.2 9.0 9.1 9.3 9.0 9.0 9.3 9.5 9.8 8.6 9.6 9.0 9.3 

 4530 8.6 8.6 8.4 8.7 9.8 8.6 8.6 8.7 8.2 8.5 7.9 8.2 8.4 9.2 8.4 8.7 

 4531 10.4 11.3 10.3 10.5 10.9 10.0 10.9 11.0 10.8 9.3 9.6 10.2 11.0 9.7 10.0 

 4532 10.6 10.6 10.9 10.9 10.6 10.4 11.3 12.2 11.1 9.8 10.9 10.0 9.3 11.2 9.8 

 4533 10.2 S 10.3 11.2 10.5 9.9 8.4 10.3 10.4 10.8 

 4534 11.6 S S 10.8 12.3 11.4 12.1 11.5 11.3 12.0 11.7 10.3 12.3 

 4535 9.8 S 9.9 10.1 9.7 9.7 10.0 8.8 10.5 10.9 10.1 9.6 9.4 9.0 9.3 9.9 10.3 

 4536 10.6 10.1 10.9 13.6 10.8 10.6 10.3 10.7 10.3 9.9 10.5 10.5 10.2 10.4 9.9 

 4537 10.7 11.0 11.0 9.7 10.8 11.6 10.5 10.8 10.6 10.3 10.9 10.4 10.8 11.4 10.6 

 4538 10.9 S 10.9 11.0 11.2 11.4 11.5 10.2 10.4 11.3 10.3 10.6 10.0 11.6 11.2 10.7 

 4539 12.1 M M 11.7 13.8 12.3 10.0 13.0 12.5 11.4 

 4540 11.3 12.2 11.6 11.8 M 10.7 11.2 11.2 11.8 11.1 11.7 11.2 10.2 11.0 

 4541 11.3 11.7 11.1 10.9 11.9 11.8 11.9 11.8 10.7 10.8 10.9 11.2 11.1 

 4542 12.0 12.9 12.5 11.0 13.0 11.3 11.2 12.1 11.4 12.7 

 4543 NOT PREG., NO MATING DATE 

 4544 NOT PREG., NO MATING DATE 

 4545 11.2 11.7 11.5 12.0 11.7 11.2 11.3 11.4 11.0 10.0 11.4 9.8 

 4546 10.6 S D S 10.6 11.5 10.9 11.0 11.1 10.0 10.3 9.9 10.1 10.9 10.2 

 4547 9.9 11.0 10.8 10.5 9.5 9.7 10.9 10.4 9.9 9.9 10.2 9.1 9.0 9.5 9.0 9.7 

 4548 9.2 8.2 9.7 9.1 8.7 9.3 9.0 9.2 9.1 9.6 9.4 9.4 9.3 9.0 10.1 

 4549 11.6 12.0 12.6 11.7 11.9 11.8 12.1 11.6 10.8 10.7 11.7 11.2 11.2 

 4550 9.5 9.4 9.7 10.0 9.7 9.6 9.7 8.8 9.6 10.1 10.2 9.3 9.7 8.2 10.0 9.2 

 4551 NOT PREGNANT 

MEAN 10.5

S.D. 0.95

N 22

PUP STATUS CODES: S-STILLBORN D-DIED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 390 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 13.7 13.6 14.2 13.7 15.0 12.5 13.4 13.9 C 13.9 13.6 13.1 C 

 1527 15.8 C 16.1 C 15.6 15.7 15.3 17.0 16.5 15.5 14.6 15.3 15.9 

 1528 14.4 16.6 16.3 16.0 7.3 16.9 16.8 D 9.6 16.0 

 1529 14.6 15.3 14.4 15.6 14.8 13.3 15.2 13.7 14.5 C 14.1 M 15.0 

 1530 18.9 19.4 19.1 18.9 18.7 18.4 

 1531 10.7 11.3 10.8 C 11.2 11.6 C C 10.5 11.0 10.6 9.1 10.0 10.5 

 1532 14.6 15.2 C C 14.4 C 15.4 16.1 14.6 14.5 14.0 14.4 13.7 13.9 

 1533 15.3 S 16.3 14.8 16.4 15.1 16.2 C 14.2 14.6 C 15.2 15.2 15.0 C C 

 1534 NOT PREGNANT 

 1535 10.0 9.7 11.1 9.4 9.7 10.7 D 9.8 9.1 10.7 9.7 10.4 

 1536 17.1 18.7 17.2 17.0 17.3 17.9 17.2 C 17.3 15.9 C 15.9 17.0 

 1537 15.6 C C C 15.4 17.3 15.8 15.7 15.7 C C 15.1 C 15.6 14.4 15.3 15.6 

 1538 NOT PREGNANT 

 1539 15.7 S C 15.6 16.1 16.8 15.7 17.0 15.5 14.5 15.2 C 16.2 14.6 

 1540 NOT PREG., NO MATING DATE 

 1541 14.7 15.1 16.1 15.3 C C 15.9 14.3 14.1 C C 13.2 14.1 14.9 14.0 M 

 1542 10.4 10.7 11.2 10.6 10.6 10.9 C 10.4 9.4 9.8 10.2 9.8 

 1543 14.1 14.9 14.9 14.9 14.5 C 14.2 C 14.5 13.5 C C 13.9 13.9 C 11.9 

 1544 15.5 S C C 16.1 16.6 15.7 15.5 15.3 15.5 14.9 14.1 15.7 15.5 

 1545 12.7 C 12.7 13.7 13.1 12.6 13.1 C C 11.8 C 13.6 13.4 12.3 10.9 C 

 1546 15.9 16.8 15.9 15.7 16.4 16.8 16.2 15.0 C 15.0 16.0 C 15.5 C 

 1547 17.9 17.6 18.6 18.3 16.7 18.0 18.2 

 1548 NOT PREGNANT 

 1549 TOTAL LITTER LOSS 

 1550 14.3 C 15.2 M 14.6 14.9 14.5 14.9 14.7 14.5 13.7 13.5 12.0 

 1551 14.3 15.4 15.1 15.2 13.7 13.8 13.7 14.5 13.6 13.5 

MEAN 14.6

S.D. 2.25

N 21

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 391 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 12.7 C C 12.8 C 13.1 C 11.9 C 14.4 13.5 13.4 C 12.4 11.2 12.1 12.5 

 2527 13.2 13.8 14.3 12.5 14.9 C 13.7 C C C 12.8 11.4 13.7 12.3 12.9 

 2528 12.2 12.2 11.8 12.1 13.4 13.2 C C 13.3 11.8 10.9 C C 11.7 11.9 C C 

 2529 13.8 C 13.8 C 14.3 14.4 14.4 14.0 C 11.8 13.5 13.9 13.9 13.7 

 2530 16.0 S 17.0 16.5 16.1 C 17.0 15.6 14.5 16.7 C C 16.1 15.1 15.6 

 2531 14.5 C 14.6 14.9 15.0 14.4 C 15.2 C 13.3 C C 14.2 13.6 M 15.2 14.3 

 2532 13.3 13.0 13.8 12.7 12.6 C 13.6 C C 13.4 13.5 14.0 13.1 12.8 

 2533 14.8 14.7 C C C 15.1 14.4 16.5 C 15.3 C 14.9 15.0 12.9 14.8 14.1 C C 

 2534 14.1 13.7 C C 15.1 C C 15.7 13.4 15.3 13.7 12.6 13.9 13.8 13.7 

 2535 16.0 15.6 15.1 15.7 17.3 16.8 15.7 15.8 16.6 16.8 14.8 

 2536 HUMANE SACRIFICE 

 2537 18.6 15.0 19.4 20.0 19.8 

 2538 15.8 15.6 15.6 16.8 D 16.9 15.9 14.0 M 15.8 15.8 15.7 

 2539 14.7 14.2 14.9 14.3 14.9 15.8 14.7 14.8 C 15.4 C C 13.7 13.8 C 

 2540 15.3 14.9 15.6 C 16.2 15.9 15.6 15.7 15.2 14.3 14.9 14.6 

 2541 13.6 13.9 14.7 14.3 13.6 13.4 12.9 13.0 13.6 12.8 13.6 C C C C 

 2542 14.5 D 14.5 19.0 14.9 13.7 14.9 C 13.4 13.6 13.8 C 13.3 14.3 

 2543 15.1 C 16.3 16.4 16.2 C 12.3 15.5 15.4 14.2 C C 15.8 C 14.6 14.4 C 

 2544 NOT PREG., NO MATING DATE 

 2545 14.1 14.1 14.8 14.3 M 14.8 13.2 14.8 14.6 13.8 C 14.6 12.5 

 2546 15.1 15.9 15.7 13.8 C 15.6 C C 14.9 15.0 16.6 14.9 13.4 C 15.3 

 2547 14.3 15.1 13.7 15.6 13.4 14.1 14.0 C 13.8 14.3 14.6 C 14.5 

 2548 15.1 15.2 16.3 16.3 15.5 C 16.5 C C 15.2 14.8 15.4 11.3 14.2 

 2549 NOT PREG., NO MATING DATE 

 2550 13.0 S 8.2 16.2 10.0 12.9 C 14.3 13.0 12.3 15.2 15.2 12.4 

 2551 15.5 16.0 16.0 15.5 15.8 15.3 15.7 C C 15.5 15.1 14.7 15.1 

MEAN 14.6

S.D. 1.36

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 392 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREG., NO MATING DATE 

 3527 13.7 C C 13.5 14.9 14.0 C 13.9 C C 14.9 12.5 12.6 13.1 14.3 13.2 C 

 3528 15.9 15.5 14.9 16.8 16.3 17.0 C C C C C 14.1 16.4 16.3 15.9 16.3 

 3529 14.7 M C 14.5 15.5 14.2 15.8 15.9 C 14.1 C 14.6 15.0 14.5 12.8 C 

 3530 NOT PREG., NO MATING DATE 

 3531 13.6 C 14.5 14.2 15.4 12.5 C 13.3 C 12.6 14.0 12.2 13.8 13.6 

 3532 15.4 C C 16.2 16.0 13.2 15.8 15.6 14.5 16.3 14.8 15.3 15.8 

 3533 12.8 13.5 C 13.1 C 12.8 13.8 12.9 12.8 12.9 C 12.1 11.7 12.2 C C 

 3534 TOTAL LITTER LOSS 

 3535 12.5 12.8 D 11.8 13.6 C 13.6 C 13.0 11.4 13.1 11.7 C C 12.8 11.5 

 3536 12.9 C 13.2 C C 13.5 13.5 13.2 13.2 12.6 12.1 12.7 12.9 12.5 

 3537 15.8 16.4 15.6 16.1 15.6 C 15.9 C 16.4 16.5 C 15.4 15.3 14.9 

 3538 15.2 15.7 C C 15.4 14.8 15.2 16.5 13.8 14.3 15.5 15.9 14.5 

 3539 13.6 13.7 13.6 11.6 14.6 14.3 C 14.8 13.0 C C 13.7 14.2 12.5 

 3540 15.2 15.4 16.1 16.3 15.9 14.6 C C 14.7 15.2 15.5 C 13.3 15.3 

 3541 16.7 18.4 17.2 18.8 15.4 18.5 C C C 10.4 C 16.1 17.2 17.5 17.8 

 3542 16.5 16.8 16.4 17.5 16.7 17.3 15.7 16.6 17.0 16.1 15.3 

 3543 13.5 14.0 14.5 13.5 14.6 11.9 C C 13.4 13.0 14.0 C 13.0 C 13.2 C 

 3544 11.6 C 11.4 11.8 12.0 12.5 12.0 12.2 C 10.8 11.9 C 9.5 12.1 

 3545 15.3 16.6 15.1 15.6 15.5 16.1 C 14.7 16.1 13.5 15.4 C C 14.4 

 3546 14.3 C C 15.4 C 14.5 13.9 15.2 14.3 13.4 13.3 13.5 15.3 14.6 C 

 3547 13.7 14.9 14.1 13.1 14.8 14.0 14.0 13.7 13.1 13.5 12.2 

 3548 16.0 16.3 16.6 15.8 16.9 16.7 C 15.4 15.6 15.5 16.0 15.6 

 3549 14.3 S 14.8 14.4 15.2 14.3 C 14.4 13.0 13.9 14.9 C C 13.4 14.2 C 

 3550 11.1 S C 11.5 11.7 C C 11.5 11.2 11.3 10.8 C C 11.1 C 10.9 11.1 10.2 

 3551 13.8 13.4 13.9 14.6 14.0 14.1 13.4 14.0 C 14.4 C C C 13.0 13.3 C 

MEAN 14.3

S.D. 1.51

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 393 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 7 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 13.6 13.5 C 13.6 14.2 C 13.4 14.1 13.3 C 13.5 12.8 13.7 14.1 

 4527 15.0 S 15.1 15.5 15.4 C 15.2 15.5 14.2 14.7 14.8 14.6 15.2 

 4528 13.2 C 13.1 C C 13.5 14.4 14.1 C 13.4 13.0 13.2 C 13.2 11.1 13.2 

 4529 13.4 13.7 14.1 12.8 C 13.1 13.3 13.4 C C C C 13.2 12.6 14.2 13.6 

 4530 13.0 13.3 13.2 12.8 C 12.7 13.2 12.8 12.3 C C C 12.9 13.9 12.9 C 

 4531 14.1 14.9 13.5 14.3 15.3 C 15.2 C 13.8 12.9 12.8 14.1 14.7 C C 

 4532 14.0 C C 15.0 13.7 C 15.1 15.1 15.2 13.1 13.8 12.3 12.4 14.4 C 

 4533 13.2 S 13.3 14.0 13.6 12.9 11.0 13.4 13.5 13.6 

 4534 15.4 S S 14.7 15.5 14.8 16.1 15.8 15.3 16.3 15.5 13.7 16.2 

 4535 13.3 S 13.7 13.9 13.3 C 13.3 12.1 C C C C 13.4 12.5 12.9 13.9 13.8 

 4536 15.9 C 16.7 18.4 16.2 16.4 15.5 15.1 15.1 15.1 15.4 C 15.4 C C 

 4537 15.3 16.3 16.0 14.8 15.5 16.4 14.2 14.7 C 14.4 15.1 C C C 15.2 

 4538 16.3 S 16.3 16.8 16.5 17.2 16.9 16.1 15.8 16.8 15.2 15.4 C C C C 

 4539 15.6 M M 15.9 17.5 16.6 12.0 16.8 16.1 14.6 

 4540 15.8 16.7 16.7 16.4 M 15.1 15.7 15.9 16.1 15.2 C C 14.9 15.2 

 4541 15.6 C C 15.9 16.1 16.3 15.6 16.0 14.5 15.2 15.3 15.0 15.6 

 4542 15.5 16.1 15.7 14.4 16.4 15.4 14.6 15.6 15.2 16.3 

 4543 NOT PREG., NO MATING DATE 

 4544 NOT PREG., NO MATING DATE 

 4545 14.4 15.0 15.1 C 15.4 14.5 14.9 14.3 14.6 12.6 14.6 12.6 

 4546 12.9 S D S 12.9 13.6 13.2 13.2 13.5 12.5 12.6 12.3 12.1 12.9 C 

 4547 14.0 C 15.8 15.3 14.0 C C C 13.5 14.2 C 13.6 13.4 13.2 13.3 13.8 

 4548 13.2 11.8 C 13.3 13.0 13.5 13.4 C 13.0 C C 12.8 13.2 13.1 15.0 

 4549 15.6 16.6 17.3 15.8 C 15.4 16.0 15.3 14.7 14.5 15.9 14.4 C 

 4550 13.4 C C C 13.6 13.9 13.5 12.2 13.3 C C 13.4 14.1 12.2 13.6 13.7 

 4551 NOT PREGNANT 

MEAN 14.3

S.D. 1.09

N 22

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 394 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 24.2 23.7 24.9 23.7 26.5 23.1 22.8 25.0 C 24.4 24.4 23.1 C 

 1527 26.4 C 26.3 C 25.8 26.1 26.2 27.3 27.2 27.9 24.2 26.5 26.8 

 1528 25.2 27.9 26.1 27.6 12.0 27.4 28.4 D D 26.8 

 1529 24.5 25.3 24.0 25.7 25.4 23.0 24.3 24.0 24.0 C 24.0 M 25.2 

 1530 34.2 35.0 33.5 34.2 34.8 33.3 

 1531 18.9 20.3 19.8 C 19.5 19.5 C C 18.6 19.5 18.7 16.8 17.3 18.7 

 1532 24.6 25.7 C C 25.7 C 25.5 26.8 24.8 25.0 22.9 23.5 23.2 23.4 

 1533 25.9 S 26.9 25.4 27.9 25.3 27.1 C 23.2 25.7 C 25.1 26.5 26.3 C C 

 1534 NOT PREGNANT 

 1535 19.8 19.0 D 18.9 18.7 21.7 D 18.9 18.7 21.0 19.5 22.0 

 1536 27.6 30.0 27.6 27.8 28.8 29.5 26.7 C 28.2 26.3 C 25.3 26.1 

 1537 28.7 C C C 28.5 30.2 28.9 27.9 28.9 C C 29.5 C 30.0 26.4 28.9 27.8 

 1538 NOT PREGNANT 

 1539 25.8 S C 25.7 25.6 28.0 25.2 27.4 26.2 24.5 25.2 C 25.4 24.9 

 1540 NOT PREG., NO MATING DATE 

 1541 27.0 28.4 28.1 26.8 C C 29.0 26.9 26.6 C C 24.9 26.4 27.6 25.4 M 

 1542 20.0 20.9 22.1 19.5 19.7 20.4 C 19.1 19.1 20.3 19.5 19.5 

 1543 25.2 26.4 25.7 26.7 25.4 C 25.1 C 25.6 25.1 C C 25.8 24.2 C 21.8 

 1544 26.3 S C C 26.6 27.7 26.7 25.8 25.6 28.0 24.9 25.2 25.7 26.5 

 1545 22.1 C 21.6 23.8 21.7 22.6 22.0 C C 21.1 C 23.1 22.5 21.9 20.3 C 

 1546 26.5 27.3 25.8 26.4 27.8 27.3 26.3 26.2 C 26.1 25.9 C 25.8 C 

 1547 28.3 27.7 28.6 29.4 27.3 28.4 28.6 

 1548 NOT PREGNANT 

 1549 TOTAL LITTER LOSS 

 1550 23.1 C 23.6 M 22.9 24.5 22.3 23.5 23.7 23.8 23.3 22.3 20.9 

 1551 24.3 26.2 24.9 25.2 24.1 23.9 23.3 24.3 23.4 23.6 

MEAN 25.2

S.D. 3.39

N 21

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 395 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 22.7 C C 22.4 C 22.4 C 22.1 C 24.9 23.5 24.3 C 22.2 20.6 22.4 21.9 

 2527 23.4 24.6 26.1 22.3 25.2 C 23.9 C C C 21.6 20.8 24.2 22.8 22.3 

 2528 22.2 23.2 23.1 22.7 23.0 23.5 C C 23.5 21.8 20.5 C C 19.9 21.0 C C 

 2529 22.0 C 21.5 C 23.2 22.5 23.1 23.3 C 20.8 19.8 21.6 22.6 21.8 

 2530 26.4 S 27.2 27.1 26.8 C 28.0 25.7 24.2 26.3 C C 26.3 26.0 26.1 

 2531 25.4 C 25.9 25.9 25.4 25.5 C 26.6 C 23.8 C C 25.7 24.9 M 26.0 24.7 

 2532 23.6 22.6 23.6 23.8 22.2 C 24.0 C C 24.4 22.9 25.0 25.0 23.0 

 2533 27.4 28.0 C C C 28.6 26.6 29.2 C 27.7 C 27.9 26.9 25.1 27.3 26.7 C C 

 2534 23.9 22.2 C C 24.9 C C 25.7 23.2 25.6 24.4 21.7 23.0 24.8 23.1 

 2535 26.0 25.6 24.4 26.5 27.6 27.0 26.7 25.6 25.7 27.0 24.3 

 2536 HUMANE SACRIFICE 

 2537 29.9 25.2 31.6 31.8 31.0 

 2538 26.3 25.2 25.2 28.2 D 27.8 26.7 23.7 M 26.3 26.5 26.7 

 2539 24.5 24.0 25.7 24.9 25.2 25.0 24.1 24.3 C 24.8 C C 23.9 23.0 C 

 2540 24.2 23.4 24.8 C 25.1 24.9 23.9 24.8 24.3 23.4 23.9 23.5 

 2541 24.6 24.9 25.0 25.1 25.6 24.9 23.1 24.3 24.5 23.0 25.3 C C C C 

 2542 23.8 D 24.9 25.1 24.8 23.2 25.3 C 22.6 23.5 21.7 C 22.5 24.0 

 2543 27.3 C 28.3 29.1 28.1 C 25.3 28.3 27.6 24.5 C C 28.3 C 27.0 26.6 C 

 2544 NOT PREG., NO MATING DATE 

 2545 26.0 26.1 26.4 27.3 M 26.7 24.4 27.7 26.9 24.4 C 27.1 23.1 

 2546 27.9 28.1 29.0 26.4 C 28.7 C C 28.6 28.3 28.5 27.1 25.6 C 29.0 

 2547 25.4 26.1 24.7 26.6 25.5 25.3 25.4 C 24.5 24.9 25.1 C 25.6 

 2548 26.4 26.7 28.5 27.5 27.0 C 27.6 C C 26.6 25.4 25.6 22.2 26.5 

 2549 NOT PREG., NO MATING DATE 

 2550 22.4 S 14.9 26.4 17.1 22.2 C 24.7 23.2 22.4 26.3 25.2 21.8 

 2551 25.9 26.7 27.4 25.9 26.1 26.8 25.4 C C 25.2 25.3 24.5 25.6 

MEAN 25.1

S.D. 2.01

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 396 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREG., NO MATING DATE 

 3527 23.5 C C 23.3 25.0 24.6 C 23.6 C C 23.3 21.5 22.6 22.9 24.1 24.0 C 

 3528 26.2 26.9 24.1 27.0 25.2 28.7 C C C C C 22.5 25.5 27.4 27.6 27.3 

 3529 24.5 M C 23.3 26.7 24.6 26.3 26.0 C 24.3 C 23.2 24.9 23.5 22.5 C 

 3530 NOT PREG., NO MATING DATE 

 3531 22.5 C 25.0 24.8 23.6 20.0 C 22.8 C 20.9 23.9 19.0 23.6 21.5 

 3532 24.9 C C 26.5 25.3 22.1 26.2 24.4 23.2 27.6 23.4 25.0 25.6 

 3533 21.5 22.8 C 21.7 C 21.4 22.8 21.0 21.8 21.3 C 20.7 21.0 20.3 C C 

 3534 TOTAL LITTER LOSS 

 3535 20.1 20.3 D 19.3 21.6 C 21.9 C 20.6 18.8 19.7 18.6 C C 21.1 18.7 

 3536 23.0 C 23.2 C C 24.9 23.1 23.6 23.3 22.9 22.0 21.7 23.8 21.8 

 3537 26.7 27.8 26.6 27.3 27.1 C 27.9 C 27.6 27.5 C 25.9 24.6 24.9 

 3538 25.6 25.2 C C 26.6 25.2 24.7 26.8 25.9 26.5 26.1 24.4 24.7 

 3539 25.9 25.8 25.4 22.5 23.3 28.5 C 28.2 26.1 C C 26.6 28.2 24.0 

 3540 25.0 25.2 26.0 27.8 26.4 25.4 C C 24.9 25.7 25.0 C 18.2 25.6 

 3541 29.3 31.2 28.9 32.1 28.0 31.4 C C C 21.6 C 28.7 31.0 30.6 29.3 

 3542 26.3 27.0 27.0 27.8 25.5 26.0 25.9 26.6 26.6 25.9 24.4 

 3543 23.1 24.1 25.8 21.8 23.2 20.4 C C 23.5 23.6 24.2 C 22.4 C 22.1 C 

 3544 21.9 C 22.0 23.0 22.2 22.9 22.0 21.7 C 20.2 22.1 C 19.7 23.4 

 3545 27.4 28.6 27.5 28.4 27.7 28.2 C 25.2 28.3 25.1 28.2 C C 26.3 

 3546 25.1 C C 26.9 C 24.3 25.4 26.6 25.6 22.9 22.0 24.5 27.2 25.9 C 

 3547 21.9 23.8 22.0 21.8 22.6 20.7 22.6 21.7 21.1 22.0 20.3 

 3548 27.4 27.2 29.2 27.0 29.4 27.7 C 26.9 26.1 27.1 27.7 25.6 

 3549 25.7 S 27.3 25.6 27.9 24.7 C 25.5 23.9 24.4 27.3 C C 25.6 24.7 C 

 3550 20.5 S C 21.4 21.0 C C 21.3 20.7 20.6 20.6 C C 20.7 C 19.0 20.7 19.1 

 3551 25.3 24.7 26.2 28.6 26.3 25.0 26.4 23.1 C 25.6 C C C 23.1 23.7 C 

MEAN 24.5

S.D. 2.37

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:56

Huntingdon Life Sciences 00-4205 PAGE 397 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 14 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 21.6 21.2 C 22.3 22.7 C 21.4 22.0 21.0 C 21.4 20.9 21.4 21.8 

 4527 26.6 S 26.9 27.2 26.9 C 26.5 27.4 25.0 26.0 26.2 25.9 28.0 

 4528 21.9 C 22.5 C C 21.9 23.2 22.4 C 21.6 22.3 21.6 C 21.8 19.2 22.4 

 4529 24.4 23.8 25.6 24.4 C 24.8 23.5 25.4 C C C C 24.4 23.0 24.7 24.8 

 4530 24.4 25.4 24.1 23.9 C 23.5 25.6 24.1 22.9 C C C 24.9 24.7 24.9 C 

 4531 23.6 24.1 23.6 23.5 24.9 C 24.6 C 23.1 22.6 22.1 23.0 24.0 C C 

 4532 23.6 C C 25.9 21.8 C 24.9 26.1 25.8 22.6 22.7 20.9 21.2 24.3 C 

 4533 22.3 S 22.2 23.7 22.6 21.6 19.7 23.1 23.2 22.6 

 4534 25.2 S S 25.1 25.2 24.1 25.3 25.3 25.5 27.3 26.1 21.7 26.7 

 4535 22.7 S 22.1 23.1 23.4 C 23.2 21.2 C C C C 22.0 22.5 22.9 23.9 22.5 

 4536 27.2 C 27.5 30.2 28.7 28.2 25.6 25.9 26.6 26.1 26.6 C 26.8 C C 

 4537 25.8 27.9 26.1 25.1 25.7 26.8 24.1 26.1 C 24.2 26.2 C C C 25.9 

 4538 28.3 S 28.2 29.4 28.4 28.9 29.5 27.2 28.9 29.4 26.2 26.6 C C C C 

 4539 24.6 M M 25.3 27.4 26.9 17.1 26.7 25.9 23.2 

 4540 27.5 28.9 29.4 29.1 M 25.5 27.0 28.2 29.2 25.9 C C 25.3 26.1 

 4541 25.3 C C 24.9 25.8 26.1 26.1 25.8 24.0 24.9 24.8 24.9 26.0 

 4542 25.0 24.9 26.3 24.2 25.8 25.0 23.6 25.4 25.0 25.1 

 4543 NOT PREG., NO MATING DATE 

 4544 NOT PREG., NO MATING DATE 

 4545 23.0 24.2 23.9 C 23.1 23.7 23.9 22.8 23.5 20.6 23.7 20.7 

 4546 22.5 S D S 22.1 23.7 22.4 23.2 23.3 21.8 22.1 22.0 21.5 22.8 C 

 4547 26.4 C 28.5 27.7 25.8 C C C 26.5 27.1 C 25.4 25.9 25.6 25.0 26.5 

 4548 21.6 17.8 C 21.9 22.2 22.0 22.3 C 21.7 C C 20.7 21.4 21.4 24.3 

 4549 26.1 26.2 29.1 26.7 C 25.4 26.0 26.2 25.2 24.5 25.6 25.8 C 

 4550 23.7 C C C 24.7 24.4 24.2 23.3 23.4 C C 23.7 24.6 22.0 23.5 23.5 

 4551 NOT PREGNANT 

MEAN 24.3

S.D. 1.82

N 22

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 398 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 41.6 42.3 43.7 40.9 45.4 39.2 39.6 43.6 C 42.1 41.2 37.6 C 

 1527 44.7 C 45.6 C 43.5 44.8 43.9 47.4 44.9 45.9 40.1 44.7 46.1 

 1528 44.5 50.5 45.9 49.7 48.3 18.6 50.8 D D 47.5 

 1529 46.2 45.7 45.4 49.5 47.4 43.7 47.1 41.3 47.3 C 45.4 M 49.3 

 1530 54.2 56.7 52.5 55.0 55.3 51.5 

 1531 31.8 31.7 32.4 C 32.3 32.9 C C 32.2 32.4 31.9 28.4 30.7 32.8 

 1532 38.4 39.4 C C 38.0 C 38.7 41.5 40.6 39.7 35.9 35.9 37.5 37.2 

 1533 44.5 S 47.3 45.8 45.4 43.1 45.9 C 40.3 44.8 C 43.7 44.8 44.3 C C 

 1534 NOT PREGNANT 

 1535 32.6 30.2 D 29.6 29.5 37.6 D 27.4 32.0 33.8 34.1 39.0 

 1536 45.6 52.1 45.1 47.5 47.6 49.3 41.7 C 47.5 41.6 C 41.6 41.5 

 1537 47.6 C C C 45.6 49.3 45.0 48.1 48.8 C C 49.3 C 50.8 44.8 46.3 47.7 

 1538 NOT PREGNANT 

 1539 43.6 S C 44.3 42.8 48.5 43.6 45.8 42.8 40.2 40.7 C 43.8 43.2 

 1540 NOT PREG., NO MATING DATE 

 1541 43.7 46.6 46.0 43.8 C C 45.5 44.1 42.9 C C 38.8 41.8 44.6 42.9 M 

 1542 32.6 33.7 36.1 32.2 33.0 35.5 C 31.0 30.3 31.7 31.9 30.7 

 1543 45.7 48.0 47.5 50.7 46.3 C 44.1 C 44.5 45.2 C C 44.7 45.9 C 40.2 

 1544 44.5 S C C 45.2 48.5 46.5 44.9 42.5 46.2 40.7 42.7 42.7 45.2 

 1545 35.0 C 29.2 39.9 34.4 35.6 35.2 C C 34.2 C 39.3 35.4 35.3 32.0 C 

 1546 43.7 44.5 44.0 42.5 44.9 44.6 42.5 44.7 C 44.8 42.9 C 42.0 C 

 1547 46.5 49.6 48.3 47.1 42.0 46.0 46.1 

 1548 NOT PREGNANT 

 1549 TOTAL LITTER LOSS 

 1550 34.5 C 32.1 M 35.0 35.4 34.3 34.5 38.7 37.3 34.9 32.0 31.0 

 1551 38.1 40.7 41.2 40.2 39.2 39.5 35.0 35.4 35.7 35.6 

MEAN 41.9

S.D. 5.89

N 21

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 399 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 35.5 C C 36.2 C 33.3 C 37.0 C 34.9 37.2 40.6 C 33.2 33.3 35.3 33.6 

 2527 37.2 37.7 41.5 33.7 39.8 C 40.2 C C C 33.6 34.2 37.4 37.1 36.7 

 2528 37.8 39.1 37.9 40.9 36.2 39.1 C C 42.0 37.6 35.3 C C 34.2 35.5 C C 

 2529 38.4 C 36.2 C 39.5 40.1 39.9 41.8 C 37.5 32.5 38.5 37.9 39.6 

 2530 45.7 S 47.8 46.4 45.1 C 51.7 44.7 41.5 44.4 C C 46.2 44.1 45.3 

 2531 42.6 C 43.5 41.8 42.1 41.7 C 43.9 C 41.6 C C 42.1 44.2 M 43.8 41.1 

 2532 41.1 40.1 41.7 38.9 38.8 C 43.0 C C 41.5 40.2 43.8 42.2 41.2 

 2533 46.5 48.5 C C C 46.5 42.5 51.3 C 45.0 C 47.9 49.0 44.9 47.8 41.8 C C 

 2534 39.7 35.8 C C 41.7 C C 40.7 35.9 44.7 39.5 38.1 40.1 40.6 40.3 

 2535 41.7 41.4 36.7 41.7 44.5 43.7 42.5 39.2 44.1 43.3 40.0 

 2536 HUMANE SACRIFICE 

 2537 52.5 43.2 53.7 60.0 52.9 

 2538 43.9 42.6 41.5 48.0 D 47.8 43.3 38.8 M 44.1 43.5 45.3 

 2539 41.6 42.1 42.3 43.2 44.9 40.6 40.9 40.9 C 43.5 C C 39.6 38.4 C 

 2540 40.9 39.3 39.6 C 44.1 39.1 42.3 42.6 41.4 40.2 40.1 39.9 

 2541 42.5 43.1 40.9 44.4 44.0 43.0 39.2 42.1 42.3 40.4 45.9 C C C C 

 2542 41.2 D 42.0 42.2 42.4 40.4 42.5 C 39.3 43.7 39.2 C 40.0 40.7 

 2543 48.5 C 49.6 53.8 49.8 C 43.9 51.7 48.6 44.0 C C 47.9 C 48.1 47.3 C 

 2544 NOT PREG., NO MATING DATE 

 2545 41.0 37.8 46.6 46.1 M 40.4 35.3 46.3 41.0 42.3 C 39.1 34.7 

 2546 49.3 50.7 49.5 48.7 C 53.2 C C 48.8 51.6 50.4 45.3 45.4 C 49.7 

 2547 38.7 39.0 38.6 42.0 39.3 39.7 38.3 C 36.8 39.4 35.9 C 38.1 

 2548 43.2 45.5 45.3 45.3 46.4 C 46.8 C C 45.0 40.6 41.3 35.6 39.8 

 2549 NOT PREG., NO MATING DATE 

 2550 37.8 S 22.4 47.9 24.7 41.5 C 41.1 37.8 39.1 43.7 43.1 36.7 

 2551 45.2 45.9 47.7 46.3 45.6 46.0 42.8 C C 45.5 44.5 42.5 45.1 

MEAN 42.3

S.D. 4.20

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 400 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 21 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREG., NO MATING DATE 

 3527 40.1 C C 40.9 41.9 41.1 C 38.5 C C 40.0 37.6 38.1 39.6 42.2 40.6 C 

 3528 44.4 44.1 39.7 47.5 44.5 47.3 C C C C C 38.6 42.1 47.3 48.5 44.7 

 3529 41.7 M C 39.7 44.8 41.9 45.6 44.4 C 41.8 C 39.0 42.3 39.1 38.7 C 

 3530 NOT PREG., NO MATING DATE 

 3531 36.7 C 44.1 38.7 43.3 28.9 C 34.7 C 33.1 40.3 27.2 40.8 35.7 

 3532 42.5 C C 46.6 42.6 35.5 42.8 41.0 40.8 48.8 39.8 41.7 45.6 

 3533 33.2 34.9 C 32.6 C 33.0 35.0 34.6 32.4 32.1 C 32.6 33.7 30.6 C C 

 3534 TOTAL LITTER LOSS 

 3535 32.0 31.2 D 31.5 32.9 C 32.6 C 34.2 31.7 32.3 31.1 C C 33.2 28.8 

 3536 40.7 C 40.5 C C 44.1 40.5 42.0 42.9 40.6 38.5 38.5 40.7 38.5 

 3537 46.3 46.7 50.3 47.3 44.8 C 46.1 C 48.8 47.8 C 46.7 41.5 43.3 

 3538 41.9 38.3 C C 44.2 42.6 39.1 43.7 42.1 M 43.3 43.8 40.1 

 3539 37.5 36.4 37.3 31.7 34.9 43.8 C 39.2 37.2 C C 37.3 44.1 32.8 

 3540 44.9 44.4 45.7 50.4 44.8 44.2 C C 42.0 45.2 43.9 C D 43.5 

 3541 51.0 54.4 49.7 56.7 50.5 51.9 C C C 38.9 C 50.0 53.4 52.7 52.0 

 3542 46.1 48.1 45.6 51.3 45.7 43.6 42.5 48.7 46.3 46.7 42.2 

 3543 38.8 41.3 44.0 35.7 38.0 30.1 C C 40.8 38.8 44.5 C 35.7 C 38.9 C 

 3544 34.5 C 32.6 36.5 33.9 37.0 33.9 37.2 C 31.1 36.0 C 31.0 35.9 

 3545 45.5 49.9 46.3 47.9 48.1 44.8 C 43.1 46.8 40.1 44.6 C C 43.4 

 3546 44.0 C C 47.2 C 43.7 45.8 46.8 46.0 42.3 40.0 40.5 43.3 44.1 C 

 3547 35.8 38.1 39.1 33.6 32.5 35.5 38.5 35.7 34.7 36.5 33.8 

 3548 46.8 46.2 50.8 45.8 50.9 49.1 C 43.9 45.4 45.6 46.9 43.1 

 3549 41.8 S 43.0 39.5 45.4 41.3 C 39.5 39.8 40.5 47.2 C C 40.2 41.1 C 

 3550 31.5 S C 32.7 32.7 C C 31.8 31.6 31.5 31.5 C C 31.9 C 28.9 32.4 30.4 

 3551 43.4 42.6 45.7 47.0 44.8 43.2 45.9 40.5 C 44.0 C C C 40.2 40.0 C 

MEAN 40.9

S.D. 5.19

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 401 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 21 

----------------------------------------------------------------------------------------------------------------------------------- 

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 35.1 33.5 C 35.9 39.3 C 34.3 33.7 35.5 C 34.4 33.9 35.8 34.6 

 4527 44.9 S 44.4 45.9 44.7 C 45.3 48.2 41.7 45.4 44.3 42.0 47.5 

 4528 38.3 C 40.6 C C 39.0 40.2 39.7 C 37.4 38.5 38.2 C 39.2 32.3 38.3 

 4529 40.2 42.0 42.0 39.2 C 38.8 42.4 41.1 C C C C 38.5 38.3 38.0 41.4 

 4530 37.8 40.0 38.2 36.1 C 35.6 37.5 37.7 35.0 C C C 38.1 39.5 40.7 C 

 4531 37.3 37.8 37.4 36.6 40.6 C 36.0 C 39.2 37.3 37.0 35.6 35.2 C C 

 4532 43.3 C C 46.6 42.2 C 46.4 46.0 46.9 40.9 40.9 38.2 38.4 46.0 C 

 4533 37.1 S 37.2 36.2 38.1 35.1 32.7 39.1 39.1 39.1 

 4534 43.8 S S 43.0 46.4 42.1 42.2 45.3 43.4 47.8 44.9 37.7 45.6 

 4535 39.3 S 40.0 40.6 42.9 C 38.6 36.3 C C C C 38.1 38.9 37.6 41.2 39.0 

 4536 48.8 C 50.4 51.3 51.2 51.2 48.3 46.9 46.1 44.3 49.6 C 48.4 C C 

 4537 44.0 48.2 40.6 42.3 45.9 45.9 38.7 44.7 C 42.5 44.2 C C C 47.4 

 4538 HUMANE SACRIFICE 

 4539 42.8 M M 43.9 50.3 46.1 24.6 46.0 46.8 42.0 

 4540 46.1 50.2 49.8 47.3 M 45.0 46.7 45.9 47.9 43.3 C C 41.4 43.7 

 4541 44.0 C C 40.4 45.6 45.2 45.1 44.7 42.3 43.7 44.4 44.8 44.3 

 4542 46.6 46.7 48.6 46.3 48.3 47.3 44.4 47.2 41.5 48.9 

 4543 NOT PREG., NO MATING DATE 

 4544 NOT PREG., NO MATING DATE 

 4545 37.6 38.2 40.9 C 37.7 37.6 39.1 35.5 41.6 33.1 38.0 34.4 

 4546 39.2 S D S 37.6 40.8 40.0 41.5 42.6 38.0 38.4 37.7 36.6 38.5 C 

 4547 44.8 C 49.9 47.0 45.8 C C C 46.7 43.7 C 44.6 39.0 43.7 44.8 43.3 

 4548 34.0 27.2 C 34.3 33.6 35.9 35.9 C 32.5 C C 33.4 34.5 33.8 38.8 

 4549 42.6 42.7 41.5 45.7 C 43.7 43.5 44.7 42.1 41.1 40.5 40.6 C 

 4550 39.7 C C C 41.5 42.9 39.2 38.2 39.4 C C 39.1 41.9 36.6 40.3 38.2 

 4551 NOT PREGNANT 

MEAN 41.2

S.D. 3.99

N 22

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 402 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP I 0 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 1526 76.7 80.7 83.9 74.0 87.3 72.2 72.1 78.0 C 75.4 75.1 68.7 C 

 1527 82.6 C 84.7 C 83.9 83.0 82.1 88.1 86.4 83.4 72.7 80.1 81.6 

 1528 81.9 94.9 85.2 93.4 35.4 91.6 85.6 D D 87.4 

 1529 82.1 81.8 87.0 92.3 84.0 76.1 82.4 73.2 82.9 C 79.1 M 82.1 

 1530 98.7 105.7 105.0 94.2 98.7 89.9 

 1531 62.2 64.0 62.7 C 66.1 65.0 C C 64.0 61.5 59.1 57.3 61.5 60.9 

 1532 77.4 79.7 C C 78.8 C 78.8 84.2 83.8 80.7 71.2 71.4 70.3 75.1 

 1533 82.5 S 87.0 83.8 86.6 82.4 86.4 C 74.3 83.2 C 81.8 80.1 79.5 C C 

 1534 NOT PREGNANT 

 1535 65.1 59.7 D 61.8 60.9 74.4 D 55.4 64.7 66.2 66.9 75.9 

 1536 80.8 90.6 79.5 85.5 83.3 89.5 76.3 C 82.7 73.0 C 76.5 71.4 

 1537 84.1 C C C 83.0 88.6 82.2 90.0 89.5 C C 83.7 C 84.9 78.5 79.2 80.9 

 1538 NOT PREGNANT 

 1539 79.4 S C 85.0 81.8 88.2 81.2 88.1 78.3 69.3 69.9 C 75.9 76.7 

 1540 NOT PREG., NO MATING DATE 

 1541 82.9 89.9 90.5 85.6 C C 86.4 87.6 79.8 C C 66.9 79.4 83.1 80.0 M 

 1542 69.6 67.2 79.1 68.6 74.5 76.4 C 66.0 69.0 63.6 66.5 64.8 

 1543 80.3 82.4 87.3 88.8 82.3 C 78.6 C 77.1 79.9 C C 76.8 78.3 C 71.7 

 1544 82.5 S C C 84.8 95.0 87.7 87.9 82.1 80.2 73.8 79.5 74.4 79.6 

 1545 70.9 C 66.7 79.7 69.9 72.7 75.3 C C 67.9 C 70.9 70.5 71.2 64.4 C 

 1546 81.0 84.6 74.9 83.6 82.9 86.2 75.9 82.6 C 81.2 80.5 C 77.8 C 

 1547 84.7 89.1 88.8 89.1 79.1 82.6 79.5 

 1548 NOT PREGNANT 

 1549 TOTAL LITTER LOSS 

 1550 71.3 C 70.4 M 72.0 73.3 70.4 73.4 76.3 75.4 72.1 67.0 62.2 

 1551 73.2 80.1 80.7 77.6 73.5 72.0 65.6 67.5 70.9 71.0 

MEAN 78.6

S.D. 7.92

N 21

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 403 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP II 2000 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 2526 73.1 C C 74.9 C 74.6 C 76.8 C 73.7 77.4 78.3 C 68.3 65.5 72.1 69.3 

 2527 71.5 76.3 80.6 67.8 76.1 C 79.7 C C C 64.4 64.4 67.9 67.7 69.8 

 2528 71.9 76.2 74.7 74.2 74.5 75.0 C C 75.5 70.4 68.7 C C 63.2 66.2 C C 

 2529 70.9 C 69.1 C 73.3 75.0 76.2 75.9 C 60.5 68.7 69.4 69.5 71.8 

 2530 83.7 S 88.1 88.5 85.3 C 95.6 82.0 75.6 81.7 C C 82.6 78.5 79.1 

 2531 78.5 C 79.6 78.0 79.9 83.5 C 81.8 C 74.5 C C 76.1 79.5 M 77.9 74.0 

 2532 77.7 79.2 77.9 77.6 75.1 C 82.7 C C 79.7 72.4 80.0 77.6 75.2 

 2533 88.3 91.2 C C C 90.0 82.6 97.0 C 86.6 C 92.3 89.1 83.2 88.1 82.8 C C 

 2534 74.2 71.6 C C 75.8 C C 76.9 73.1 82.4 69.9 72.2 73.1 76.1 70.8 

 2535 80.1 83.7 69.8 81.3 84.8 83.9 82.6 78.1 81.5 80.6 74.7 

 2536 HUMANE SACRIFICE 

 2537 91.4 77.3 93.8 105.5 88.9 

 2538 82.8 81.2 84.5 92.6 D 89.7 87.2 70.6 M 77.9 77.4 84.1 

 2539 76.7 80.8 76.4 81.5 81.1 82.6 71.1 71.7 C 80.0 C C 69.6 72.5 C 

 2540 77.0 74.3 74.1 C 85.1 76.9 80.4 80.2 78.7 73.8 75.4 71.5 

 2541 77.6 79.5 80.5 81.2 80.8 77.0 69.0 77.3 75.8 73.7 81.6 C C C C 

 2542 79.1 D 83.8 80.9 82.9 77.2 84.6 C 77.3 80.0 73.8 C 75.0 75.5 

 2543 86.9 C 93.2 97.1 92.9 C 82.1 93.4 83.0 78.4 C C 83.1 C 84.0 81.8 C 

 2544 NOT PREG., NO MATING DATE 

 2545 80.0 73.1 91.1 90.8 M 80.5 67.9 84.4 80.2 81.3 C 78.1 72.8 

 2546 90.2 96.5 90.8 91.0 C 99.0 C C 90.2 91.8 90.7 78.7 83.5 C 90.1 

 2547 68.3 71.2 72.0 73.4 71.6 69.7 62.3 C 66.9 64.6 65.6 C 66.0 

 2548 79.7 84.4 84.2 85.0 84.6 C 84.2 C C 79.0 75.7 80.2 65.3 74.3 

 2549 NOT PREG., NO MATING DATE 

 2550 68.9 S 42.3 89.6 46.6 70.9 C 74.0 70.6 69.1 75.6 81.1 68.9 

 2551 84.5 86.0 90.2 90.5 87.3 82.2 79.8 C C 81.5 84.5 80.6 82.7 

MEAN 78.8

S.D. 6.60

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 404 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP III 10000 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NOT PREG., NO MATING DATE 

 3527 73.0 C C 74.4 78.5 74.8 C 73.9 C C 73.0 69.8 69.5 69.4 76.8 69.4 C 

 3528 83.3 83.7 77.2 88.2 88.3 88.5 C C C C C 76.9 77.2 84.4 85.8 82.7 

 3529 78.8 M C 79.2 86.0 80.8 88.8 85.7 C 77.3 C 70.8 75.8 71.5 72.2 C 

 3530 NOT PREG., NO MATING DATE 

 3531 65.3 C 81.9 78.2 80.3 41.9 C 61.5 C 65.6 73.2 34.6 73.0 62.3 

 3532 76.9 C C 80.7 82.7 67.1 79.6 73.6 75.3 83.8 70.4 74.6 81.3 

 3533 62.6 68.1 C 65.7 C 61.7 68.9 62.5 59.5 57.2 C 61.5 60.9 60.3 C C 

 3534 TOTAL LITTER LOSS 

 3535 59.5 60.4 D 59.0 62.0 C 60.8 C 64.3 59.4 56.6 57.7 C C 58.8 55.9 

 3536 73.5 C 77.3 C C 80.1 73.6 76.5 76.9 73.2 70.5 68.1 71.6 67.2 

 3537 83.4 86.0 89.8 86.3 78.2 C 83.6 C 88.7 89.0 C 80.5 76.0 75.8 

 3538 77.8 77.5 C C 83.3 78.4 73.1 84.6 73.5 M 77.3 77.5 75.1 

 3539 72.1 72.8 75.5 61.3 71.5 85.2 C 74.9 69.0 C C 67.4 77.8 65.4 

 3540 83.7 85.1 85.6 93.0 87.8 85.3 C C 77.0 79.6 79.5 C D 80.2 

 3541 91.4 100.7 93.2 98.1 95.0 98.4 C C C 68.8 C 85.1 91.5 93.9 89.8 

 3542 83.6 87.2 87.0 93.7 82.9 81.7 80.9 85.3 80.4 83.6 73.7 

 3543 74.9 80.0 83.4 71.8 76.1 64.2 C C 75.3 71.7 81.5 C 71.6 C 73.2 C 

 3544 68.6 C 70.2 74.3 68.6 73.3 68.8 70.5 C 64.6 68.5 C 61.0 65.9 

 3545 84.5 92.3 85.6 91.3 90.1 85.7 C 82.1 83.2 74.3 81.6 C C 78.4 

 3546 81.1 C C 87.7 C 81.6 89.9 86.7 86.8 76.7 73.9 71.0 77.5 79.6 C 

 3547 70.3 76.1 75.3 69.0 66.9 73.0 78.4 67.5 63.7 70.0 63.4 

 3548 81.9 82.3 92.3 81.6 91.9 86.1 C 77.6 76.0 77.2 83.5 70.1 

 3549 78.7 S 80.8 78.3 86.9 78.4 C 77.7 73.9 74.7 84.0 C C 74.0 78.3 C 

 3550 64.8 S C 69.0 67.0 C C 63.8 69.6 65.2 60.4 C C 69.6 C 60.6 60.6 62.6 

 3551 83.9 82.7 85.5 89.3 89.1 88.6 88.1 79.3 C 82.4 C C C 78.1 76.1 C 

MEAN 76.2

S.D. 8.22

N 23

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:53

Huntingdon Life Sciences 00-4205 PAGE 405 

 

 APPENDIX U F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP BODY WEIGHTS (GRAMS) 

GROUP IV 20000 MG/M3 LACTATION DAY 28 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

 4526 70.2 69.2 C 73.4 77.7 C 69.4 68.5 70.6 C 66.3 70.0 70.7 65.9 

 4527 81.0 S 82.3 80.0 81.0 C 82.0 91.0 73.1 84.1 72.9 77.7 85.4 

 4528 69.6 C 72.6 C C 73.3 74.4 73.9 C 69.5 68.3 68.6 C 68.7 58.5 68.1 

 4529 78.7 84.2 86.0 77.1 C 76.2 83.4 81.6 C C C C 73.4 74.5 72.8 77.3 

 4530 73.7 79.2 73.4 73.3 C 71.6 76.6 73.0 69.1 C C C 70.6 73.2 76.9 C 

 4531 72.1 72.2 75.1 71.9 77.8 C 74.0 C 71.4 71.3 69.8 68.3 69.0 C C 

 4532 80.6 C C 85.5 80.5 C 83.9 88.0 92.5 74.7 74.7 74.1 69.6 82.5 C 

 4533 68.9 S 74.7 64.3 73.1 68.2 64.3 69.9 67.8 69.2 

 4534 80.4 S S 81.1 85.4 79.0 80.8 84.9 82.2 83.6 79.6 67.3 80.4 

 4535 72.5 S 71.8 70.7 80.6 C 74.0 70.0 C C C C 67.3 72.8 70.5 74.5 72.4 

 4536 86.9 C 91.5 94.1 89.4 95.0 89.5 86.1 79.5 73.5 86.1 C 84.4 C C 

 4537 82.2 93.1 70.6 80.6 87.0 87.0 71.4 79.9 C 83.9 81.7 C C C 87.0 

 4538 HUMANE SACRIFICE 

 4539 80.3 M M 84.0 95.6 83.9 50.9 85.6 84.2 77.8 

 4540 85.0 93.7 90.6 90.1 M 84.2 88.1 80.3 84.3 79.6 C C 77.2 82.2 

 4541 78.9 C C 70.7 81.4 87.9 84.4 84.1 69.4 77.3 77.2 76.7 79.4 

 4542 84.7 86.4 92.7 84.0 89.5 89.1 79.1 80.0 76.8 85.0 

 4543 NOT PREG., NO MATING DATE 

 4544 NOT PREG., NO MATING DATE 

 4545 71.5 72.4 78.8 C 73.5 75.7 75.5 70.4 74.5 61.5 69.3 63.6 

 4546 74.5 S D S 72.3 82.3 78.3 80.7 82.5 71.0 70.2 69.7 68.3 70.1 C 

 4547 81.9 C 88.1 89.8 85.7 C C C 84.9 87.5 C 81.5 69.6 76.7 78.3 76.9 

 4548 67.6 58.6 C 70.6 67.8 75.5 69.3 C 62.8 C C 64.0 65.7 66.9 74.8 

 4549 79.5 79.1 81.6 85.9 C 88.2 78.4 77.4 76.8 79.0 73.6 75.2 C 

 4550 71.2 C C C 75.3 80.7 72.8 68.5 69.9 C C 65.3 75.1 64.0 71.3 68.7 

 4551 NOT PREGNANT 

MEAN 76.9

S.D. 5.85

N 22

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED M-MISSING 
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 406 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 NO REMARKABLE OBSERVATIONS 

 1027 NO REMARKABLE OBSERVATIONS 

 1028 NO REMARKABLE OBSERVATIONS 

 1029 EXTERNAL EXAM ALOPECIA - GENERAL 

 EXTERNAL FINDING NOT CONFIRMED 

 1030 NO REMARKABLE OBSERVATIONS 

 1031 LIVER DISCOLORED FOCI; SLIGHT 

 MEDIAN LOBE - 0.1 CM IN DIAMETER, RED 

 1032 NO REMARKABLE OBSERVATIONS 

 1033 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, LESS THAN 0.1 CM IN DIAMETER, TAN 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 1034 NO REMARKABLE OBSERVATIONS 

 1035 NO REMARKABLE OBSERVATIONS 

 1036 LIVER DISCOLORED AREA(S); SLIGHT 

 MEDIAN LOBE - 1.0 CM IN DIAMETER, BROWN 

 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 1037 NO REMARKABLE OBSERVATIONS 

 1038 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 407 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1039 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 1040 NO REMARKABLE OBSERVATIONS 

 1041 NO REMARKABLE OBSERVATIONS 

 1042 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 EXTERNAL EXAM SWOLLEN DIGIT 

 FIRST DIGIT, LEFT FOREPAW 

EXTERNAL FINDING CONFIRMED 

TISSUE SAVED 

 1043 NO REMARKABLE OBSERVATIONS 

 1044 NO REMARKABLE OBSERVATIONS 

 1045 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 1046 NO REMARKABLE OBSERVATIONS 

 1047 NO REMARKABLE OBSERVATIONS 

 1048 NO REMARKABLE OBSERVATIONS 

 1049 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 1050 THYMUS DISCOLORED FOCUS/FOCI; MODERATE; BILATERAL 

 0.2 CM IN DIAMETER, RED 

TISSUE SAVED 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 408 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1051 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 409 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 2027 NO REMARKABLE OBSERVATIONS 

 2028 NO REMARKABLE OBSERVATIONS 

 2029 NO REMARKABLE OBSERVATIONS 

 2030 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 0.1 CM IN DIAMETER, RED 

 2031 LUNGS DISCOLORED FOCI; SLIGHT 

 RIGHT AND LEFT LOBES, RED, 0.2 CM IN DIAMETER 

 2032 NO REMARKABLE OBSERVATIONS 

 2033 GONADS DISCOLORED FOCI ON PROSTATE; SLIGHT 

 RIGHT DORSAL LOBE - 0.1 CM IN DIAMETER, RED 

 2034 KIDNEY DILATED RENAL PELVIS; EXTREME; LEFT 

 2035 NO REMARKABLE OBSERVATIONS 

 2036 KIDNEY DILATED RENAL PELVIS; MODERATE; LEFT 

 2037 NO REMARKABLE OBSERVATIONS 

 2038 NO REMARKABLE OBSERVATIONS 

 2039 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 410 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2040 NO REMARKABLE OBSERVATIONS 

 2041 NO REMARKABLE OBSERVATIONS 

 2042 NO REMARKABLE OBSERVATIONS 

 2043 NO REMARKABLE OBSERVATIONS 

 2044 THYMUS DISCOLORED FOCUS/FOCI; EXTREME; BILATERAL 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 2045 NO REMARKABLE OBSERVATIONS 

 2046 NO REMARKABLE OBSERVATIONS 

 2047 KIDNEY DILATED RENAL PELVIS; EXTREME; RIGHT 

 2048 NO REMARKABLE OBSERVATIONS 

 2049 THYMUS DISCOLORED FOCUS/FOCI; SLIGHT 

 RED, 0.1 - 0.2 CM DIAMETER 

 TISSUE SAVED 

 2050 THYMUS DISCOLORED FOCUS/FOCI; MODERATE; BILATERAL 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 2051 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 0.1 CM IN DIAMETER, RED 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 411 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 NO REMARKABLE OBSERVATIONS 

 3027 NO REMARKABLE OBSERVATIONS 

 3028 NO REMARKABLE OBSERVATIONS 

 3029 THYMUS DISCOLORED FOCUS/FOCI; MODERATE; LEFT 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 3030 NO REMARKABLE OBSERVATIONS 

 3031 NO REMARKABLE OBSERVATIONS 

 3032 NO REMARKABLE OBSERVATIONS 

 3033 NO REMARKABLE OBSERVATIONS 

 3034 NO REMARKABLE OBSERVATIONS 

 3035 NO REMARKABLE OBSERVATIONS 

 3036 NO REMARKABLE OBSERVATIONS 

 3037 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 3038 KIDNEY DILATED RENAL PELVIS; MODERATE; BILATERAL 

 3039 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 412 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3040 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - 0.1 CM IN DIAMETER, RED 

 LIVER ADHESION; EXTREME 

 MEDIAN AND RIGHT LOBES TO DIAPHRAGM 

 LIVER NODULE(S) 

 MEDIAN LOBE - 0.5 CM IN DIAMETER, TAN, FIRM 

 NODULE SAVED WITH PART OF DIAPHRAGM 

 3041 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, WHITE, 0.2 CM IN DIAMETER 

 3042 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 3043 HS GROSS EXAM FRACTURE(S) 

 NASAL BONES 

TISSUE SAVED 

 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

EXTERNAL EXAM ORAL SORE 

 HARD PALATE, WITH SCABS, 0.4 CM IN DIAMETER 

TISSUE SAVED 

EXTERNAL FINDING CONFIRMED 

 3044 DIED THYMUS DISCOLORED FOCUS/FOCI; MODERATE 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 SPLEEN ENLARGED; EXTREME 

LIVER ENLARGED; EXTREME 

 ALL LOBES 

 3045 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD HS=HUMANE SACRIFICE 
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 413 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3046 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 3047 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 3048 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 3049 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 3050 KIDNEY CYSTIC KIDNEY; SLIGHT; LEFT 

 CORTEX, CLEAR 0.2 CM IN DIAMETER 

 3051 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 414 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 NO REMARKABLE OBSERVATIONS 

 4027 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 4028 LUNGS DISCOLORED FOCI; SLIGHT 

 DIAPHRAGMATIC LOBE -0.1 CM IN DIAMETER, RED 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 4029 NO REMARKABLE OBSERVATIONS 

 4030 NO REMARKABLE OBSERVATIONS 

 4031 NO REMARKABLE OBSERVATIONS 

 4032 THYMUS DISCOLORED FOCUS/FOCI; SLIGHT; BILATERAL 

 RED, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 4033 NO REMARKABLE OBSERVATIONS 

 4034 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 4035 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, WHITE, 0.1-0.5 CM IN DIAMETER 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 4036 GONADS DISCOLORED FOCI ON PROSTATE; MODERATE 

 RIGHT VENTRAL LOBE - 0.4 CM IN DIAMETER, RED 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 415 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4037 NO REMARKABLE OBSERVATIONS 

 4038 NO REMARKABLE OBSERVATIONS 

 4039 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 4040 NO REMARKABLE OBSERVATIONS 

 4041 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 4042 NO REMARKABLE OBSERVATIONS 

 4043 NO REMARKABLE OBSERVATIONS 

 4044 KIDNEY DISCOLORED FOCUS/FOCI; SLIGHT; RIGHT 

 CORTEX - 0.2 CM IN DIAMETER, BROWN 

 4045 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 4046 NO REMARKABLE OBSERVATIONS 

 4047 NO REMARKABLE OBSERVATIONS 

 4048 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:58

Huntingdon Life Sciences 00-4205 PAGE 416 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4049 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 4050 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - 0.1 CM IN DIAMETER, TAN 

 4051 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 18:00

Huntingdon Life Sciences 00-4205 PAGE 417 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE ESTRUS 

 1527 ESTROUS CYCLE DIESTRUS 

 1528 ESTROUS CYCLE ESTRUS 

 1529 ESTROUS CYCLE DIESTRUS 

 1530 ESTROUS CYCLE ESTRUS 

 1531 THORACIC CAVITY ENLARGED LYMPH NODE(S); MODERATE 

 SUBMANDULAR 

 TISSUE SAVED 

 EXTERNAL EXAM ALOPECIA - GENERAL 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 1532 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 ESTROUS CYCLE DIESTRUS 

 1533 ESTROUS CYCLE ESTRUS 

 1534 NP EXTERNAL EXAM RED EXUDATE SNOUT 

 NEGATIVE 

 EXTERNAL EXAM MISSING DIGIT 

 EXTERNAL FINDING CONFIRMED 

 SALEWSKI TEST NO FOCI PRESENT 

 ESTROUS CYCLE DIESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT 
11-JAN-2010 18:00

Huntingdon Life Sciences 00-4205 PAGE 418 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1535 ESTROUS CYCLE DIESTRUS 

 1536 ESTROUS CYCLE DIESTRUS 

 1537 ESTROUS CYCLE DIESTRUS 

 1538 NP ESTROUS CYCLE DIESTRUS 

 SALEWSKI TEST NO FOCI PRESENT 

 1539 ESTROUS CYCLE DIESTRUS 

 1540 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE DIESTRUS 

 1541 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 1542 ESTROUS CYCLE DIESTRUS 

 1543 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1-0.2 CM IN DIAMETER 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE ESTRUS 

 1544 ADRENALS DISCOLORED ADRENALS; SLIGHT; RIGHT 

 RED 

 ESTROUS CYCLE DIESTRUS 

 1545 ESTROUS CYCLE DIESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE 
11-JAN-2010 18:00

Huntingdon Life Sciences 00-4205 PAGE 419 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1546 ESTROUS CYCLE DIESTRUS 

 1547 ESTROUS CYCLE DIESTRUS 

 1548 NP EXTERNAL EXAM RED EXUDATE SNOUT 

 NEGATIVE 

 SALEWSKI TEST NO FOCI PRESENT 

ESTROUS CYCLE ESTRUS 

 1549 TLL EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE ESTRUS 

 1550 ESTROUS CYCLE PROESTRUS 

 1551 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 ESTROUS CYCLE ESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT TLL=TOTAL LITTER LOSS 
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 420 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 EYES OPACITY; RIGHT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE PROESTRUS 

 2527 ESTROUS CYCLE ESTRUS 

 2528 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDINGS CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 2529 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - 0.1 CM IN DIAMETER, TAN 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE METESTRUS 

 2530 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE ESTRUS 

 2531 ESTROUS CYCLE DIESTRUS 

 2532 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 2533 ESTROUS CYCLE DIESTRUS 

 2534 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, WHITE, 0.2 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 421 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2535 ESTROUS CYCLE ESTRUS 

 2536 HS GROSS EXAM FRACTURE(S) 

 NASAL BONE - BILATERAL 

SKULL SAVED 

 EXTERNAL EXAM INCISORS BROKEN/MISSING 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 2537 ESTROUS CYCLE DIESTRUS 

 2538 ESTROUS CYCLE DIESTRUS 

 2539 LUNGS DISCOLORED FOCI; SLIGHT 

 DIAPHRAGMATIC LOBE - 0.1 CM IN DIAMETER, RED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 2540 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES, 0.1 CM IN DIAMETER, RED 

 ESTROUS CYCLE ESTRUS 

 2541 ESTROUS CYCLE METESTRUS 

 2542 ESTROUS CYCLE ESTRUS 

 2543 PNMD ESTROUS CYCLE DIESTRUS 

 2544 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE DIESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE 
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 422 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2545 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 2546 ESTROUS CYCLE DIESTRUS 

 2547 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 ESTROUS CYCLE DIESTRUS 

 2548 EXTERNAL EXAM ALOPECIA - GENERAL 

 EXTERNAL FINDING NOT CONFIRMED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE ESTRUS 

 2549 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE DIESTRUS 

 2550 ESTROUS CYCLE DIESTRUS 

 2551 ESTROUS CYCLE ESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE 
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 423 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3526 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE ESTRUS 

 3527 ESTROUS CYCLE ESTRUS 

 3528 ESTROUS CYCLE DIESTRUS 

 3529 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1-0.2 CM IN DIAMETER 

 ESTROUS CYCLE ESTRUS 

 3530 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE ESTRUS 

 3531 ESTROUS CYCLE DIESTRUS 

 3532 ESTROUS CYCLE DIESTRUS 

 3533 LUNGS DISCOLORED FOCI; SLIGHT; LEFT 

 LEFT LOBE, 0.1-0.2 CM IN DIAMETER, WHITE 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 3534 TLL LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 3535 ESTROUS CYCLE ESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS 
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 424 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3536 ESTROUS CYCLE DIESTRUS 

 3537 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE PROESTRUS 

 3538 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 3539 ESTROUS CYCLE DIESTRUS 

 3540 ESTROUS CYCLE ESTRUS 

 3541 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 3542 THYMUS DISCOLORED FOCUS/FOCI; SLIGHT 

 BOTH LOBES, 0.1 - 0.2 CM IN DIAMETER, RED 

 TISSUE SAVED 

 ESTROUS CYCLE DIESTRUS 

 3543 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 ESTROUS CYCLE DIESTRUS 

 3544 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE METESTRUS 

 3545 ESTROUS CYCLE DIESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 425 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3546 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 3547 ESTROUS CYCLE DIESTRUS 

 3548 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - 0.1 CM IN DIAMETER, RED AND TAN 

 ESTROUS CYCLE DIESTRUS 

 3549 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, LESS THAN 0.1 CM IN DIAMETER, WHITE 

 ESTROUS CYCLE ESTRUS 

 3550 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 3551 ESTROUS CYCLE ESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 426 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 ESTROUS CYCLE DIESTRUS 

 4527 ESTROUS CYCLE DIESTRUS 

 4528 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE ESTRUS 

 4529 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE PROESTRUS 

 4530 PNMD LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.2 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 4531 SPLEEN ADHESION; EXTREME 

 TO STOMACH WALL AND PANCREAS 

TISSUE SAVED 

 ESTROUS CYCLE DIESTRUS 

 4532 LUNGS DISCOLORED FOCI; MODERATE 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 4533 ESTROUS CYCLE DIESTRUS 

 4534 ESTROUS CYCLE DIESTRUS 

 4535 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, 0.1 CM IN DIAMETER 

 EXTERNAL EXAM ALOPECIA - GENERAL 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM ALOPECIA - EXTREMITIES/SNOUT 

 EXTERNAL FINDING CONFIRMED 

-----------------------------------------------------------------------------------------------------------------------------------

PNMD=PREGNANT, NO MATING DATE 
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 427 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4535 (CONTINUED) 

 ESTROUS CYCLE DIESTRUS 

 4536 ESTROUS CYCLE DIESTRUS 

 4537 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - TAN, LESS THAN 0.1 CM IN DIAMETER 

 ESTROUS CYCLE METESTRUS 

 4538 HS GROSS EXAM HEMORRHAGE; MODERATE 

 SKULL - LEFT FRONTAL/NASAL BONES, RED, 1 CM IN DIAMETER 

 SKULL SAVED 

 EXTERNAL EXAM RED EXUDATE SNOUT 

 EXTERNAL FINDING CONFIRMED 

 EXTERNAL EXAM SWOLLEN SNOUT 

 EXTERNAL FINDING CONFIRMED 

TISSUE SAVED 

 EXTERNAL EXAM INCISORS MALOCCLUDED 

 EXTERNAL FINDING CONFIRMED 

 ESTROUS CYCLE DIESTRUS 

 4539 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, TAN, 0.2 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 4540 ESTROUS CYCLE DIESTRUS 

 4541 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, LESS THEN 0.1 CM IN DIAMETER, TAN 

 ESTROUS CYCLE ESTRUS 

 4542 ESTROUS CYCLE DIESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

 HS=HUMANE SACRIFICE 
11-JAN-2010 17:59

Huntingdon Life Sciences 00-4205 PAGE 428 

 

 APPENDIX V P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4543 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE DIESTRUS 

 4544 NNMD EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE ESTRUS 

 4545 ESTROUS CYCLE ESTRUS 

 4546 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES - LESS THAN 0.1 CM IN DIAMETER, TAN 

 ESTROUS CYCLE DIESTRUS 

 4547 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, 0.1-0.2 CM IN DIAMETER, WHITE 

 ESTROUS CYCLE DIESTRUS 

 4548 ESTROUS CYCLE ESTRUS 

 4549 ESTROUS CYCLE ESTRUS 

 4550 LUNGS DISCOLORED FOCI; SLIGHT 

 ALL LOBES, WHITE, 0.1-0.2 CM IN DIAMETER 

 ESTROUS CYCLE DIESTRUS 

 4551 NP EXTERNAL EXAM SALEWSKI'S TEST 

 NO FOCI PRESENT 

 ESTROUS CYCLE ESTRUS 

-----------------------------------------------------------------------------------------------------------------------------------

 NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 429 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 6F K 28 ABDOMINAL CAVITY DIAPHRAGMATIC HERNIA 

 PART OF MEDIAN LOBE PROTRUDING THROUGH DIAPHRAGM 

1.0 x 0.5 CM 

TISSUE SAVED 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 1527 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 1528 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 8F D 11 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 9F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 430 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1529 1M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 1530 1M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3F K 28 NO REMARKABLE OBSERVATIONS 

 4F K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 1531 1M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUES SAVED 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 1532 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 431 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1532 (CONTINUED) 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 1533 1F S GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 1535 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M D 8 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F D 1 GROSS EXAM AUTOLYSIS 

 POSTMORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 1536 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 432 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

----------------------------------------------------------------------------------------------------------------------------------- 

 1536 (CONTINUED) 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 1537 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 1539 1M S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 1541 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 7M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 433 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1541 (CONTINUED) 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 1542 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 1543 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 1544 1M S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 434 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1544 (CONTINUED) 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 1545 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 3M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 6M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUE SAVED 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 1546 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 1547 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 435 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1549 TLL 1F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 3M D 2 GROSS EXAM CANNIBALIZED 

 4M D 2 STOMACH MILK IN STOMACH 

 5M D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 6M D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 7M D 2 STOMACH NO MILK IN STOMACH 

 8M D 2 STOMACH NO MILK IN STOMACH 

 9F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 10F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 11F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 12F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 13F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 14F D 2 GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 1550 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 KIDNEY CYST(S); SLIGHT; RIGHT 

 CORTEX, CLEAR, 0.1 CM IN DIAMETER 

TISSUE SAVED 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 THYMUS SMALL; SLIGHT 

 TISSUE SAVED 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE 

TLL=TOTAL LITTER LOSS 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 436 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 1551 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4F K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 437 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 11M K 28 NO REMARKABLE OBSERVATIONS 

 12M C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 2527 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 2528 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 438 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2528 (CONTINUED) 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 2529 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2530 1F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 2531 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 4M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 439 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2531 (CONTINUED) 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 2532 1M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUE SAVED 

 3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2533 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 17F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 440 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2534 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 2535 2M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 2537 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4F K 28 NO REMARKABLE OBSERVATIONS 

 2538 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M D 0 GROSS EXAM CANNIBALIZED 

 LUNGS LUNG FLOTATION TEST - FOUND DEAD 

 STOMACH MILK IN STOMACH 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 441 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2539 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 5M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 2540 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 2541 1M K 28 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 TISSUE SAVED 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 442 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2542 1M D 0 LUNGS LUNG FLOTATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 2543 PNMD 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 2545 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 

PNMD=PREGNANT, NO MATING DATE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 443 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2546 1M K 28 KIDNEY CYST(S); SLIGHT; LEFT 

 CORTEX - CLEAR, 0.1 CM IN DIAMETER 

 TISSUE SAVED 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 2547 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 2548 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 444 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 2550 1F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 2551 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 445 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 16F C 4 NO REMARKABLE OBSERVATIONS 

 3528 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 3529 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 446 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3529 (CONTINUED) 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 3531 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 EYES OPACITY; MODERATE; BILATERAL 

 TISSUE SAVED 

 EXTERNAL EXAM DOMED HEAD 

 EXTERNAL FINDING CONFIRMED 

EXTREME INTERNAL HYDROCEPHALY, EXTREME DECREASE IN CORTICAL 

THICKNESS, LATERAL VENTRICLES FILLED WITH DISCOLORED (RED) 

FLUID, CEREBELLUM AND MEDULLA OBLANGATA MISSHAPEN 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 EXTERNAL EXAM DOMED HEAD 

 EXTERNAL FINDING CONFIRMED 

 EXTREME INTERNAL HYDROCEPHALY, EXTREME DECREASE IN CORTICAL 

 THICKNESS, LATERAL VENTRICALS FILLED WITH DISCOLORED (RED) 

 FLUID, CEREBELLUM MISSHAPEN 

TISSUE SAVED 

 3532 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 447 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3533 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 3534 TLL 1F D 1 LUNGS LUNG FLOTATION TEST - FOUND DEAD 

 STOMACH NO MILK IN STOMACH 

KIDNEY ENLARGED KIDNEY(S); MODERATE; BILATERAL 

 2F D 1 GROSS EXAM CANNIBALIZED 

 GROSS EXAM SEX UNDETERMINED 

GROSS EXAM AUTOLYSIS 

 POST-MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 3535 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M D 1 GROSS EXAM AUTOLYSIS 

 POSTMORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12M C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 

TLL=TOTAL LITTER LOSS 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 448 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3536 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 3537 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3538 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 3539 1M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 449 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3539 (CONTINUED) 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3540 1M K 28 KIDNEY CYST(S); SLIGHT; RIGHT 

 CORTEX - 0.1 CM IN DIAMETER, CLEAR 

TISSUE SAVED 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 KIDNEY CYST(S); SLIGHT; BILATERAL 

 CORTEX - 0.1 CM IN DIAMETER, CLEAR 

TISSUES SAVED 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F D 16 HEAD V DOMED HEAD; MODERATE 

 EXTREME INTERNAL HYDROCEPHALY 

 EYES OPACITY; BILATERAL 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3541 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 KIDNEY CYST(S); EXTREME; LEFT 

 CORTEX, MULTIPLE, 0.2-2.0 CM IN DIAMETER, CLEAR 

 TISSUE SAVED 

 KIDNEY DILATED RENAL PELVIS; EXTREME; LEFT 

 KIDNEY DISCOLORED; EXTREME; LEFT 

 PALE 

 TISSUE SAVED 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: D-DIED C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 450 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3541 (CONTINUED) 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 3542 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUE SAVED 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 3543 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 3544 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 451 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3545 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F C 4 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 3546 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 3547 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 3548 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT 

 TISSUE SAVED 

 6F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 452 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3548 (CONTINUED) 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 3549 1F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 3550 1F S GROSS EXAM AUTOLYSIS 

 POSTMORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 17F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 453 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 3551 2M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 454 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 4527 1F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 4528 1M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 455 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4529 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11M C 4 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 4530 PNMD 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUE SAVED 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 4531 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 

PNMD=PREGNANT, NO MATING DATE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 456 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4531 (CONTINUED) 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 4532 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 4533 1M S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 STOMACH SMALL; EXTREME 

 KIDNEY M FUSED KIDNEY 

 INTESTINES A UNDERDEVELOPMENT OF INTESTINE 

 INTESTINES REDUCED TO A SMALL MASS. 

 INTESTINES ANAL ATRESIA 

 GONADS UNDESCENDED TESTE(S); RIGHT 

ABDOMINAL CAVITY GASTROSCHISIS 

 FISSURE OF ABDOMINAL WALL AT THE MEDIAN LINE. 

SMALL PART OF INTESTINES PROTUDE. 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 457 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4534 1M S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 9M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 4535 1M S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M C 4 NO REMARKABLE OBSERVATIONS 

 9M C 4 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 11M C 4 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 16F K 28 NO REMARKABLE OBSERVATIONS 

 4536 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 458 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4536 (CONTINUED) 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 4537 1M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL 

 TISSUES SAVED 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F C 4 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 4538 HS 1U S GROSS EXAM CANNIBALIZED 

 GROSS EXAM SEX UNDETERMINED 

 2M U 14 NO REMARKABLE OBSERVATIONS 

 3M U 14 NO REMARKABLE OBSERVATIONS 

 4M U 14 NO REMARKABLE OBSERVATIONS 

 5M U 14 NO REMARKABLE OBSERVATIONS 

 6M U 14 NO REMARKABLE OBSERVATIONS 

 7F U 14 NO REMARKABLE OBSERVATIONS 

 8F U 14 NO REMARKABLE OBSERVATIONS 

 9F U 14 NO REMARKABLE OBSERVATIONS 

 10F U 14 NO REMARKABLE OBSERVATIONS 

 11F U 14 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 13F C 4 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 15F C 4 NO REMARKABLE OBSERVATIONS 

 4539 3M K 28 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN C-CULLED K-SCHEDULED SACRIFICE U-UNSCHEDULED SACRIFICE 

 HS=HUMANE SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 459 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4539 (CONTINUED) 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 4540 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 4541 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 4M K 28 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT 

 TISSUE SAVED 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 4542 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6F K 28 NO REMARKABLE OBSERVATIONS 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 4545 1M K 28 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 460 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4545 (CONTINUED) 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 4546 1F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 2F D 0 STOMACH NO MILK IN STOMACH 

 LUNGS LUNG FLOTATION TEST - FOUND DEAD 

 3F S LUNGS LUNG FLOTATION TEST-STILLBORN 

 STOMACH NO MILK IN STOMACH 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 14F C 4 NO REMARKABLE OBSERVATIONS 

 4547 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M K 28 NO REMARKABLE OBSERVATIONS 

 3M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 5M C 4 NO REMARKABLE OBSERVATIONS 

 6M C 4 NO REMARKABLE OBSERVATIONS 

 7M C 4 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 10M C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 15F K 28 NO REMARKABLE OBSERVATIONS 

 4548 1M K 28 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: S-STILLBORN D-DIED C-CULLED K-SCHEDULED SACRIFICE 
22-JAN-2010 12:33

Huntingdon Life Sciences 00-4205 PAGE 461 

 

 APPENDIX W F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP NECROPSY OBSERVATIONS 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION 

-----------------------------------------------------------------------------------------------------------------------------------

 4548 (CONTINUED) 

 6M K 28 NO REMARKABLE OBSERVATIONS 

 7F C 4 NO REMARKABLE OBSERVATIONS 

 8F K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 11F K 28 NO REMARKABLE OBSERVATIONS 

 12F K 28 THYMUS DISCOLORED FOCI; MODERATE 

 BOTH LOBES - 0.1 CM IN DIAMETER, RED 

 TISSUE SAVED 

 4549 3M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT 

 TISSUE SAVED 

 4M C 4 NO REMARKABLE OBSERVATIONS 

 5M K 28 NO REMARKABLE OBSERVATIONS 

 6M K 28 GONADS UNDESCENDED TESTE(S); LEFT 

 TISSUE SAVED 

 7F K 28 NO REMARKABLE OBSERVATIONS 

 9F K 28 NO REMARKABLE OBSERVATIONS 

 10F K 28 NO REMARKABLE OBSERVATIONS 

 12F C 4 NO REMARKABLE OBSERVATIONS 

 4550 1M C 4 NO REMARKABLE OBSERVATIONS 

 2M C 4 NO REMARKABLE OBSERVATIONS 

 3M C 4 NO REMARKABLE OBSERVATIONS 

 4M K 28 NO REMARKABLE OBSERVATIONS 

 7M K 28 NO REMARKABLE OBSERVATIONS 

 8M K 28 NO REMARKABLE OBSERVATIONS 

 9F C 4 NO REMARKABLE OBSERVATIONS 

 10F C 4 NO REMARKABLE OBSERVATIONS 

 12F K 28 NO REMARKABLE OBSERVATIONS 

 13F K 28 NO REMARKABLE OBSERVATIONS 

 14F K 28 NO REMARKABLE OBSERVATIONS 

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M-Male, F=Female, U=Undetermined

PUP STATUS CODES: C-CULLED K-SCHEDULED SACRIFICE 
Huntingdon Life Sciences 00-4205 Page 462

211-DIPE-1G Final Report

Individual Parental Organ Weights

Preface Appendix X

Key to Abbreviations:

g = Grams

FBW = Final Body Weight

SEM. VES & CO.GL. = Seminal Vesicles and Coagulating Glands

NOTES:

1. Due to an apparent weighing error, the right epididymis weight for Animal 

No. 2043 is not presented in the following appendix.

2. Due to computer limitation, values for %FBW less than 0.0001 do not 

appear in this appendix.
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 463 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TESTIS - RIGHT TESTIS - LEFT TESTES PROSTATE 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 596 1.7993 0.0030 0.8163 1.7939 0.0030 0.8139 3.5711 0.0060 1.6201 1.0581 0.0018 0.4800 

 1027 595 1.7557 0.0030 0.7673 1.7465 0.0029 0.7633 3.4946 0.0059 1.5273 1.0416 0.0018 0.4552 

 1028 554 1.6881 0.0030 0.7468 1.6812 0.0030 0.7437 3.3187 0.0060 1.4681 1.7335 0.0031 0.7669 

 1029 522 1.5302 0.0029 0.7426 1.5624 0.0030 0.7582 3.0878 0.0059 1.4985 0.7730 0.0015 0.3751 

 1030 574 1.7717 0.0031 0.7913 1.8094 0.0032 0.8081 3.5800 0.0062 1.5989 1.5824 0.0028 0.7067 

 1031 547 1.5122 0.0028 0.7255 1.4277 0.0026 0.6849 2.9311 0.0054 1.4062 1.0058 0.0018 0.4825 

 1032 621 1.8170 0.0029 0.7711 1.8152 0.0029 0.7703 3.6322 0.0058 1.5414 1.5616 0.0025 0.6627 

 1033 561 1.9301 0.0034 0.8254 1.9521 0.0035 0.8348 3.8788 0.0069 1.6588 0.6683 0.0012 0.2858 

 1034 512 1.4359 0.0028 0.7017 1.5098 0.0030 0.7378 2.9438 0.0058 1.4385 1.2219 0.0024 0.5971 

 1035 535 1.8372 0.0034 0.8900 1.8268 0.0034 0.8850 3.6543 0.0068 1.7703 1.4368 0.0027 0.6961 

 1036 597 1.8088 0.0030 0.8104 1.9348 0.0032 0.8668 3.7108 0.0062 1.6625 1.4726 0.0025 0.6598 

 1037 522 1.5800 0.0030 0.7539 1.5592 0.0030 0.7439 3.1302 0.0060 1.4935 1.1181 0.0021 0.5335 

 1038 582 1.5886 0.0027 0.7629 1.5947 0.0027 0.7658 3.1793 0.0055 1.5267 1.0151 0.0017 0.4875 

 1039 510 1.5938 0.0031 0.7586 1.5508 0.0030 0.7381 3.1318 0.0061 1.4906 1.1260 0.0022 0.5359 

 1040 661 1.8173 0.0027 0.8110 1.7817 0.0027 0.7952 3.5884 0.0054 1.6015 1.4564 0.0022 0.6500 

 1041 683 1.8513 0.0027 0.8461 1.8152 0.0027 0.8296 3.6553 0.0054 1.6705 1.0450 0.0015 0.4776 

 1042 687 1.7695 0.0026 0.7792 1.7796 0.0026 0.7837 3.5494 0.0052 1.5630 1.0055 0.0015 0.4428 

 1043 445 1.7503 0.0039 0.8071 1.6880 0.0038 0.7783 3.4333 0.0077 1.5831 1.0918 0.0025 0.5034 

 1044 635 1.5616 0.0025 0.7607 1.6105 0.0025 0.7845 3.1681 0.0050 1.5432 1.3382 0.0021 0.6519 

 1045 555 1.7361 0.0031 0.8638 1.7920 0.0032 0.8916 3.5117 0.0063 1.7472 1.2788 0.0023 0.6363 

 1046 594 1.6286 0.0027 0.7228 1.5760 0.0027 0.6994 3.2102 0.0054 1.4247 1.4828 0.0025 0.6581 

 1047 530 1.7351 0.0033 0.8288 1.7488 0.0033 0.8353 3.4905 0.0066 1.6673 1.1052 0.0021 0.5279 

 1048 625 1.4819 0.0024 0.6557 1.4681 0.0023 0.6496 2.9271 0.0047 1.2952 1.2858 0.0021 0.5689 

 1049 605 1.9999 0.0033 0.9230 2.0042 0.0033 0.9250 4.0212 0.0066 1.8558 1.3470 0.0022 0.6217 

 1050 597 1.7741 0.0030 0.7531 1.7452 0.0029 0.7408 3.5344 0.0059 1.5003 0.9414 0.0016 0.3996 

 1051 505 1.6026 0.0032 0.7953 1.5959 0.0032 0.7919 3.2030 0.0063 1.5894 1.2348 0.0024 0.6127 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 464 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TESTIS - RIGHT TESTIS - LEFT TESTES PROSTATE 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 505 1.5245 0.0030 0.7001 1.6044 0.0032 0.7367 3.1258 0.0062 1.4354 1.0736 0.0021 0.4930 

 2027 511 1.6583 0.0032 0.7244 1.7121 0.0033 0.7479 3.3680 0.0066 1.4713 1.0069 0.0020 0.4398 

 2028 567 1.7076 0.0030 0.7873 1.6956 0.0030 0.7817 3.3713 0.0060 1.5543 1.5233 0.0027 0.7023 

 2029 588 1.6714 0.0028 0.7867 1.6794 0.0029 0.7905 3.3367 0.0057 1.5706 1.1011 0.0019 0.5183 

 2030 625 1.6981 0.0027 0.7488 1.6291 0.0026 0.7184 3.3269 0.0053 1.4671 1.1333 0.0018 0.4998 

 2031 516 1.7142 0.0033 0.8084 1.6127 0.0031 0.7605 3.3238 0.0064 1.5675 1.3548 0.0026 0.6389 

 2032 492 1.8586 0.0038 0.8846 1.7882 0.0036 0.8511 3.6445 0.0074 1.7346 1.1312 0.0023 0.5384 

 2033 639 1.5580 0.0024 0.7042 1.6148 0.0025 0.7299 3.1729 0.0050 1.4341 1.0875 0.0017 0.4915 

 2034 608 1.7149 0.0028 0.8006 1.6876 0.0028 0.7879 3.4010 0.0056 1.5878 1.1032 0.0018 0.5151 

 2035 504 1.6905 0.0034 0.8342 1.6329 0.0032 0.8058 3.3173 0.0066 1.6370 0.9001 0.0018 0.4442 

 2036 491 1.7842 0.0036 0.7794 1.7415 0.0035 0.7607 3.4955 0.0071 1.5270 1.2491 0.0025 0.5456 

 2037 572 1.8589 0.0032 0.8302 1.9142 0.0033 0.8549 3.7319 0.0065 1.6668 1.2487 0.0022 0.5577 

 2038 540 1.6706 0.0031 0.7739 1.7581 0.0033 0.8144 3.4002 0.0063 1.5750 1.4708 0.0027 0.6813 

 2039 582 1.4696 0.0025 0.6710 1.6069 0.0028 0.7337 3.0614 0.0053 1.3978 1.1126 0.0019 0.5080 

 2040 581 1.6926 0.0029 0.8062 1.6572 0.0029 0.7894 3.3441 0.0058 1.5929 1.5279 0.0026 0.7278 

 2041 614 1.8165 0.0030 0.8434 1.8179 0.0030 0.8440 3.6254 0.0059 1.6832 0.9897 0.0016 0.4595 

 2042 551 1.5531 0.0028 0.7493 1.5118 0.0027 0.7294 3.0543 0.0055 1.4737 1.0920 0.0020 0.5269 

 2043 640 1.6935 0.0026 0.7678 1.6895 0.0026 0.7660 3.3691 0.0053 1.5275 1.2020 0.0019 0.5450 

 2044 674 1.6068 0.0024 0.7526 1.5856 0.0024 0.7427 3.1837 0.0047 1.4913 0.8473 0.0013 0.3969 

 2045 664 1.6855 0.0025 0.7908 1.6646 0.0025 0.7810 3.3423 0.0050 1.5681 1.1005 0.0017 0.5163 

 2046 563 1.5739 0.0028 0.7481 1.5524 0.0028 0.7379 3.1279 0.0056 1.4867 1.3885 0.0025 0.6600 

 2047 598 1.6604 0.0028 0.7845 1.6592 0.0028 0.7840 3.3100 0.0055 1.5640 1.2626 0.0021 0.5966 

 2048 495 1.7914 0.0036 0.8384 1.8012 0.0036 0.8430 3.5840 0.0072 1.6774 1.0083 0.0020 0.4719 

 2049 697 1.7536 0.0025 0.7800 1.7164 0.0025 0.7634 3.4671 0.0050 1.5421 1.1086 0.0016 0.4931 

 2050 606 1.5748 0.0026 0.7172 1.5947 0.0026 0.7262 3.1389 0.0052 1.4294 0.9434 0.0016 0.4296 

 2051 545 1.6132 0.0030 0.7408 1.6850 0.0031 0.7738 3.2996 0.0061 1.5153 0.7812 0.0014 0.3588 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 465 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TESTIS - RIGHT TESTIS - LEFT TESTES PROSTATE 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

----------------------------------------------------------------------------------------------------------------------------------- 

 3026 575 1.6386 0.0028 0.7780 1.6814 0.0029 0.7983 3.3205 0.0058 1.5766 0.9738 0.0017 0.4624 

 3027 485 1.7334 0.0036 0.8623 1.7602 0.0036 0.8757 3.4877 0.0072 1.7351 0.9520 0.0020 0.4736 

 3028 625 1.8099 0.0029 0.8058 1.7912 0.0029 0.7975 3.5665 0.0057 1.5879 1.7572 0.0028 0.7823 

 3029 611 1.6481 0.0027 0.7617 1.5417 0.0025 0.7125 3.1848 0.0052 1.4719 1.3883 0.0023 0.6416 

 3030 627 1.9387 0.0031 0.8891 1.9716 0.0031 0.9042 3.8900 0.0062 1.7841 1.3376 0.0021 0.6135 

 3031 524 1.5786 0.0030 0.7600 1.5739 0.0030 0.7578 3.1412 0.0060 1.5124 1.1755 0.0022 0.5660 

 3032 510 1.5368 0.0030 0.7155 1.6427 0.0032 0.7648 3.1535 0.0062 1.4682 1.0800 0.0021 0.5028 

 3033 587 1.6921 0.0029 0.7995 1.6596 0.0028 0.7842 3.3457 0.0057 1.5808 1.6520 0.0028 0.7806 

 3034 594 1.7101 0.0029 0.7528 1.7133 0.0029 0.7542 3.3816 0.0057 1.4886 0.8583 0.0014 0.3778 

 3035 624 2.0352 0.0033 0.8802 2.0943 0.0034 0.9057 4.1095 0.0066 1.7772 1.5748 0.0025 0.6811 

 3036 505 1.7243 0.0034 0.8159 1.7844 0.0035 0.8443 3.4736 0.0069 1.6435 1.8031 0.0036 0.8531 

 3037 577 1.7958 0.0031 0.8231 1.7626 0.0031 0.8079 3.5381 0.0061 1.6217 1.2175 0.0021 0.5581 

 3038 569 1.7906 0.0031 0.8153 1.8088 0.0032 0.8236 3.5855 0.0063 1.6326 1.3860 0.0024 0.6311 

 3039 568 1.8495 0.0033 0.8419 1.8858 0.0033 0.8584 3.7134 0.0065 1.6904 0.9385 0.0017 0.4272 

 3040 660 1.6888 0.0026 0.7879 1.7724 0.0027 0.8269 3.4591 0.0052 1.6138 1.2118 0.0018 0.5653 

 3041 629 1.5568 0.0025 0.7240 1.5861 0.0025 0.7376 3.1365 0.0050 1.4586 1.3628 0.0022 0.6337 

 3042 553 1.7494 0.0032 0.8242 1.7364 0.0031 0.8181 3.4694 0.0063 1.6345 1.0542 0.0019 0.4967 

 3045 476 1.6271 0.0034 0.7336 1.6651 0.0035 0.7507 3.2985 0.0069 1.4872 1.0570 0.0022 0.4766 

 3046 536 1.8476 0.0034 0.8550 1.8307 0.0034 0.8472 3.6691 0.0068 1.6979 1.1521 0.0021 0.5331 

 3047 559 1.9826 0.0035 0.9524 2.0046 0.0036 0.9630 3.9827 0.0071 1.9133 1.3078 0.0023 0.6283 

 3048 559 1.6910 0.0030 0.8038 1.6290 0.0029 0.7743 3.3218 0.0059 1.5790 1.2461 0.0022 0.5923 

 3049 550 1.9390 0.0035 0.8704 1.9360 0.0035 0.8691 3.8820 0.0071 1.7427 1.0790 0.0020 0.4844 

 3050 660 1.6530 0.0025 0.6948 1.6370 0.0025 0.6881 3.2650 0.0049 1.3724 1.2808 0.0019 0.5384 

 3051 646 1.7778 0.0028 0.8149 1.7567 0.0027 0.8052 3.5284 0.0055 1.6173 1.3625 0.0021 0.6245 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 466 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 TESTIS - RIGHT TESTIS - LEFT TESTES PROSTATE 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 642 1.7615 0.0027 0.8302 1.7261 0.0027 0.8135 3.4808 0.0054 1.6404 1.3245 0.0021 0.6242 

 4027 512 1.5946 0.0031 0.7306 1.5652 0.0031 0.7172 3.1547 0.0062 1.4455 1.2075 0.0024 0.5533 

 4028 597 1.9704 0.0033 0.9385 1.8835 0.0032 0.8971 3.8503 0.0065 1.8339 0.8864 0.0015 0.4222 

 4029 657 1.8850 0.0029 0.8429 1.9263 0.0029 0.8613 3.7879 0.0058 1.6937 1.0052 0.0015 0.4495 

 4030 591 1.7362 0.0029 0.7963 1.8032 0.0031 0.8270 3.5260 0.0060 1.6172 1.2066 0.0020 0.5534 

 4031 504 1.7098 0.0034 0.7742 1.6729 0.0033 0.7574 3.3677 0.0067 1.5248 1.2415 0.0025 0.5621 

 4032 783 1.5324 0.0020 0.7186 1.4908 0.0019 0.6991 3.0010 0.0038 1.4073 1.1647 0.0015 0.5462 

 4033 530 1.8109 0.0034 0.8655 1.7504 0.0033 0.8366 3.5416 0.0067 1.6927 1.4101 0.0027 0.6739 

 4034 553 1.7124 0.0031 0.8533 1.6687 0.0030 0.8315 3.3608 0.0061 1.6747 1.1010 0.0020 0.5486 

 4035 498 1.7213 0.0035 0.8608 1.7647 0.0035 0.8825 3.4675 0.0070 1.7341 1.2432 0.0025 0.6217 

 4036 596 1.8719 0.0031 0.8477 1.8484 0.0031 0.8370 3.7217 0.0062 1.6853 0.8787 0.0015 0.3979 

 4037 550 1.7254 0.0031 0.8475 1.8409 0.0033 0.9043 3.5455 0.0065 1.7416 1.8010 0.0033 0.8847 

 4038 520 1.7512 0.0034 0.8566 1.7068 0.0033 0.8349 3.4405 0.0066 1.6830 1.7594 0.0034 0.8606 

 4039 552 2.0844 0.0038 1.0057 2.0478 0.0037 0.9881 4.1307 0.0075 1.9931 1.0967 0.0020 0.5292 

 4040 552 1.8674 0.0034 0.8782 1.8224 0.0033 0.8570 3.6792 0.0067 1.7302 1.4292 0.0026 0.6721 

 4041 530 1.6517 0.0031 0.7851 1.6291 0.0031 0.7743 3.2755 0.0062 1.5569 0.7926 0.0015 0.3767 

 4042 595 1.7660 0.0030 0.8193 1.6963 0.0028 0.7870 3.4475 0.0058 1.5995 0.9881 0.0017 0.4584 

 4043 521 1.6245 0.0031 0.7721 1.6510 0.0032 0.7847 3.2654 0.0063 1.5521 0.8865 0.0017 0.4214 

 4044 541 1.7284 0.0032 0.8179 1.8509 0.0034 0.8759 3.5761 0.0066 1.6923 1.0462 0.0019 0.4951 

 4045 566 1.8256 0.0032 0.8568 1.7698 0.0031 0.8307 3.5931 0.0064 1.6864 1.4511 0.0026 0.6811 

 4046 578 1.6690 0.0029 0.7309 1.6812 0.0029 0.7362 3.3158 0.0057 1.4521 1.1782 0.0020 0.5160 

 4047 608 1.9350 0.0032 0.9329 1.8670 0.0031 0.9001 3.8087 0.0063 1.8362 1.3182 0.0022 0.6355 

 4048 655 1.8074 0.0028 0.7686 1.8386 0.0028 0.7819 3.6364 0.0056 1.5464 1.4425 0.0022 0.6134 

 4049 534 1.6480 0.0031 0.7864 1.6113 0.0030 0.7689 3.2636 0.0061 1.5573 1.2141 0.0023 0.5793 

 4050 463 1.6948 0.0037 0.8261 1.6150 0.0035 0.7872 3.3129 0.0071 1.6149 1.0667 0.0023 0.5200 

 4051 551 1.8084 0.0033 0.8324 1.8410 0.0033 0.8474 3.6360 0.0066 1.6736 0.8900 0.0016 0.4097 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 467 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 596 1.9912 0.0033 0.9034 0.6269 0.0011 0.2844 0.6412 0.0011 0.2909 1.2402 0.0021 0.5627 

 1027 595 1.6469 0.0028 0.7198 0.7436 0.0012 0.3250 0.7397 0.0012 0.3233 1.4763 0.0025 0.6452 

 1028 554 1.2242 0.0022 0.5416 0.6839 0.0012 0.3025 0.7512 0.0014 0.3323 1.3579 0.0025 0.6007 

 1029 522 2.0439 0.0039 0.9919 0.6781 0.0013 0.3291 0.6742 0.0013 0.3272 1.3496 0.0026 0.6550 

 1030 574 1.6700 0.0029 0.7459 0.7653 0.0013 0.3418 0.7351 0.0013 0.3283 1.5009 0.0026 0.6703 

 1031 547 1.5901 0.0029 0.7629 0.6676 0.0012 0.3203 0.6184 0.0011 0.2967 1.2868 0.0024 0.6173 

 1032 621 1.4172 0.0023 0.6014 0.7334 0.0012 0.3112 0.8023 0.0013 0.3405 1.5344 0.0025 0.6512 

 1033 561 1.8686 0.0033 0.7991 0.7273 0.0013 0.3110 0.7151 0.0013 0.3058 1.4413 0.0026 0.6164 

 1034 512 1.9693 0.0038 0.9623 0.5679 0.0011 0.2775 0.5757 0.0011 0.2813 1.1448 0.0022 0.5594 

 1035 535 1.9474 0.0036 0.9434 0.7740 0.0014 0.3750 0.7168 0.0013 0.3473 1.4971 0.0028 0.7253 

 1036 597 2.7034 0.0045 1.2112 0.7566 0.0013 0.3390 0.7185 0.0012 0.3219 1.4455 0.0024 0.6476 

 1037 522 1.8864 0.0036 0.9000 0.6120 0.0012 0.2920 0.6408 0.0012 0.3057 1.2492 0.0024 0.5960 

 1038 582 2.3289 0.0040 1.1184 0.7701 0.0013 0.3698 0.7055 0.0012 0.3388 1.4664 0.0025 0.7042 

 1039 510 1.6997 0.0033 0.8090 0.6275 0.0012 0.2987 0.6298 0.0012 0.2998 1.2484 0.0024 0.5942 

 1040 661 2.0372 0.0031 0.9092 0.7811 0.0012 0.3486 0.8497 0.0013 0.3792 1.6089 0.0024 0.7180 

 1041 683 1.8921 0.0028 0.8647 0.6679 0.0010 0.3052 0.6946 0.0010 0.3174 1.3486 0.0020 0.6163 

 1042 687 2.1539 0.0031 0.9485 0.6861 0.0010 0.3021 0.6686 0.0010 0.2944 1.3447 0.0020 0.5921 

 1043 445 1.8358 0.0041 0.8465 0.7571 0.0017 0.3491 0.7503 0.0017 0.3460 1.5008 0.0034 0.6920 

 1044 635 1.9786 0.0031 0.9638 0.6915 0.0011 0.3368 0.6902 0.0011 0.3362 1.3677 0.0022 0.6662 

 1045 555 1.7824 0.0032 0.8868 0.6592 0.0012 0.3280 0.6985 0.0013 0.3475 1.3545 0.0024 0.6739 

 1046 594 2.3070 0.0039 1.0238 0.7106 0.0012 0.3154 0.7428 0.0013 0.3296 1.4110 0.0024 0.6262 

 1047 530 2.0412 0.0039 0.9750 0.7321 0.0014 0.3497 0.7514 0.0014 0.3589 1.4803 0.0028 0.7071 

 1048 625 1.8270 0.0029 0.8084 0.7523 0.0012 0.3329 0.7723 0.0012 0.3417 1.5026 0.0024 0.6649 

 1049 605 2.4220 0.0040 1.1178 0.8184 0.0014 0.3777 0.7337 0.0012 0.3386 1.5494 0.0026 0.7151 

 1050 597 1.6624 0.0028 0.7057 0.7205 0.0012 0.3058 0.6800 0.0011 0.2886 1.4068 0.0024 0.5972 

 1051 505 1.1790 0.0023 0.5851 0.6572 0.0013 0.3261 0.5699 0.0011 0.2828 1.2310 0.0024 0.6109 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 468 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 505 1.5743 0.0031 0.7229 0.6916 0.0014 0.3176 0.6495 0.0013 0.2983 1.3370 0.0026 0.6140 

 2027 511 1.1878 0.0023 0.5189 0.7026 0.0014 0.3069 0.6810 0.0013 0.2975 1.3813 0.0027 0.6034 

 2028 567 2.0938 0.0037 0.9653 0.7844 0.0014 0.3616 0.8253 0.0015 0.3805 1.5779 0.0028 0.7275 

 2029 588 2.3799 0.0041 1.1202 0.8067 0.0014 0.3797 0.7917 0.0013 0.3727 1.5701 0.0027 0.7390 

 2030 625 2.1051 0.0034 0.9283 0.7894 0.0013 0.3481 0.6914 0.0011 0.3049 1.4708 0.0024 0.6486 

 2031 516 2.3005 0.0045 1.0849 0.6846 0.0013 0.3228 0.6554 0.0013 0.3091 1.3385 0.0026 0.6312 

 2032 492 1.7739 0.0036 0.8443 0.8290 0.0017 0.3946 0.7301 0.0015 0.3475 1.5541 0.0032 0.7397 

 2033 639 2.4335 0.0038 1.0999 0.6734 0.0011 0.3044 0.6630 0.0010 0.2997 1.3314 0.0021 0.6018 

 2034 608 2.1199 0.0035 0.9897 0.7231 0.0012 0.3376 0.7394 0.0012 0.3452 1.4605 0.0024 0.6819 

 2035 504 1.6221 0.0032 0.8004 0.6751 0.0013 0.3331 0.7346 0.0015 0.3625 1.4098 0.0028 0.6957 

 2036 491 1.6809 0.0034 0.7343 0.7494 0.0015 0.3274 0.7547 0.0015 0.3297 1.4823 0.0030 0.6475 

 2037 572 1.8144 0.0032 0.8104 0.7693 0.0013 0.3436 0.7425 0.0013 0.3316 1.4773 0.0026 0.6598 

 2038 540 2.5517 0.0047 1.1820 0.8472 0.0016 0.3924 0.8116 0.0015 0.3759 1.6193 0.0030 0.7501 

 2039 582 1.7870 0.0031 0.8159 0.7270 0.0012 0.3319 0.7817 0.0013 0.3569 1.5015 0.0026 0.6856 

 2040 581 2.0468 0.0035 0.9749 0.7143 0.0012 0.3402 0.7383 0.0013 0.3517 1.4503 0.0025 0.6908 

 2041 614 1.5050 0.0025 0.6987 0.6854 0.0011 0.3182 0.6819 0.0011 0.3166 1.3568 0.0022 0.6299 

 2042 551 1.2961 0.0024 0.6253 0.7620 0.0014 0.3677 0.6562 0.0012 0.3166 1.4041 0.0026 0.6775 

 2043 640 1.3546 0.0021 0.6142 0.7383 0.0012 0.3347 1.4822 0.0023 0.6720 

 2044 674 1.8257 0.0027 0.8552 0.7243 0.0011 0.3393 0.7241 0.0011 0.3392 1.4317 0.0021 0.6706 

 2045 664 1.6518 0.0025 0.7749 0.7756 0.0012 0.3639 0.8130 0.0012 0.3814 1.5797 0.0024 0.7411 

 2046 563 1.5639 0.0028 0.7433 0.6716 0.0012 0.3192 0.6782 0.0012 0.3224 1.3470 0.0024 0.6402 

 2047 598 2.3298 0.0039 1.1008 0.7565 0.0013 0.3574 0.7166 0.0012 0.3386 1.4613 0.0024 0.6905 

 2048 495 1.8268 0.0037 0.8550 0.7508 0.0015 0.3514 0.6497 0.0013 0.3041 1.3899 0.0028 0.6505 

 2049 697 2.2349 0.0032 0.9940 0.8032 0.0012 0.3572 0.7712 0.0011 0.3430 1.5735 0.0023 0.6999 

 2050 606 1.9345 0.0032 0.8810 0.6150 0.0010 0.2801 0.6659 0.0011 0.3032 1.2516 0.0021 0.5700 

 2051 545 1.5891 0.0029 0.7298 0.6427 0.0012 0.2952 0.6348 0.0012 0.2915 1.2756 0.0023 0.5858 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 469 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 575 2.4182 0.0042 1.1482 0.6871 0.0012 0.3262 0.7432 0.0013 0.3529 1.4230 0.0025 0.6757 

 3027 485 1.8991 0.0039 0.9448 0.7503 0.0015 0.3733 0.7429 0.0015 0.3696 1.4951 0.0031 0.7438 

 3028 625 1.8973 0.0030 0.8447 0.7838 0.0013 0.3490 0.7725 0.0012 0.3439 1.5956 0.0026 0.7104 

 3029 611 2.3883 0.0039 1.1038 0.6996 0.0011 0.3233 0.6984 0.0011 0.3228 1.3928 0.0023 0.6437 

 3030 627 1.9354 0.0031 0.8876 0.8390 0.0013 0.3848 0.8147 0.0013 0.3736 1.6355 0.0026 0.7501 

 3031 524 1.9386 0.0037 0.9334 0.6610 0.0013 0.3182 0.6359 0.0012 0.3062 1.2939 0.0025 0.6230 

 3032 510 1.6818 0.0033 0.7830 0.6917 0.0014 0.3221 0.6894 0.0014 0.3210 1.3418 0.0026 0.6247 

 3033 587 2.6938 0.0046 1.2728 0.8131 0.0014 0.3842 0.7644 0.0013 0.3612 1.5583 0.0027 0.7363 

 3034 594 2.1860 0.0037 0.9623 0.7115 0.0012 0.3132 0.7308 0.0012 0.3217 1.4099 0.0024 0.6206 

 3035 624 1.2161 0.0019 0.5259 0.8112 0.0013 0.3508 0.8053 0.0013 0.3483 1.5870 0.0025 0.6863 

 3036 505 2.2141 0.0044 1.0476 0.7873 0.0016 0.3725 0.8849 0.0018 0.4187 1.6551 0.0033 0.7831 

 3037 577 1.9245 0.0033 0.8821 0.7387 0.0013 0.3386 0.7654 0.0013 0.3508 1.4826 0.0026 0.6796 

 3038 569 1.9414 0.0034 0.8840 0.8297 0.0015 0.3778 0.8247 0.0014 0.3755 1.6418 0.0029 0.7476 

 3039 568 1.6585 0.0029 0.7550 0.6849 0.0012 0.3118 0.7092 0.0012 0.3228 1.3853 0.0024 0.6306 

 3040 660 1.6262 0.0025 0.7587 0.7137 0.0011 0.3330 0.6658 0.0010 0.3106 1.3679 0.0021 0.6382 

 3041 629 1.8279 0.0029 0.8500 0.7670 0.0012 0.3567 0.7426 0.0012 0.3453 1.5053 0.0024 0.7000 

 3042 553 2.2894 0.0041 1.0786 0.8028 0.0015 0.3782 0.7604 0.0014 0.3582 1.5579 0.0028 0.7340 

 3045 476 1.9320 0.0041 0.8711 0.7408 0.0016 0.3340 0.7358 0.0015 0.3317 1.4820 0.0031 0.6682 

 3046 536 2.0079 0.0037 0.9292 0.7533 0.0014 0.3486 0.7906 0.0015 0.3658 1.5304 0.0029 0.7082 

 3047 559 1.9578 0.0035 0.9405 0.7413 0.0013 0.3561 0.7573 0.0014 0.3638 1.4945 0.0027 0.7180 

 3048 559 2.0985 0.0038 0.9975 0.8176 0.0015 0.3886 0.7500 0.0013 0.3565 1.5735 0.0028 0.7480 

 3049 550 2.0285 0.0037 0.9106 0.7298 0.0013 0.3276 0.7147 0.0013 0.3208 1.4578 0.0027 0.6544 

 3050 660 2.4739 0.0037 1.0398 0.7779 0.0012 0.3270 0.7514 0.0011 0.3158 1.5138 0.0023 0.6363 

 3051 646 1.6624 0.0026 0.7620 0.7684 0.0012 0.3522 0.7657 0.0012 0.3510 1.5449 0.0024 0.7081 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 470 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 642 2.0008 0.0031 0.9429 0.7907 0.0012 0.3726 0.7237 0.0011 0.3411 1.5060 0.0023 0.7097 

 4027 512 2.5156 0.0049 1.1526 0.6395 0.0012 0.2930 0.6410 0.0013 0.2937 1.3574 0.0027 0.6219 

 4028 597 2.2210 0.0037 1.0579 0.7286 0.0012 0.3470 0.6792 0.0011 0.3235 1.4035 0.0024 0.6685 

 4029 657 2.3201 0.0035 1.0374 0.8528 0.0013 0.3813 0.7951 0.0012 0.3555 1.6361 0.0025 0.7316 

 4030 591 2.1304 0.0036 0.9771 0.6953 0.0012 0.3189 0.6217 0.0011 0.2851 1.3098 0.0022 0.6007 

 4031 504 3.4055 0.0068 1.5419 0.7411 0.0015 0.3356 0.7113 0.0014 0.3221 1.4518 0.0029 0.6573 

 4032 783 2.4017 0.0031 1.1262 0.8013 0.0010 0.3758 0.7876 0.0010 0.3693 1.5583 0.0020 0.7307 

 4033 530 1.9443 0.0037 0.9293 0.8036 0.0015 0.3841 0.7698 0.0015 0.3679 1.5456 0.0029 0.7387 

 4034 553 1.6766 0.0030 0.8355 0.6797 0.0012 0.3387 0.7009 0.0013 0.3493 1.3578 0.0025 0.6766 

 4035 498 2.1931 0.0044 1.0968 0.7348 0.0015 0.3675 0.7814 0.0016 0.3908 1.4935 0.0030 0.7469 

 4036 596 2.0301 0.0034 0.9193 0.6817 0.0011 0.3087 0.7351 0.0012 0.3329 1.4138 0.0024 0.6402 

 4037 550 2.2986 0.0042 1.1291 0.7853 0.0014 0.3857 0.8463 0.0015 0.4157 1.6075 0.0029 0.7896 

 4038 520 2.0347 0.0039 0.9953 0.8352 0.0016 0.4086 0.7394 0.0014 0.3617 1.5515 0.0030 0.7589 

 4039 552 2.3981 0.0043 1.1571 0.8748 0.0016 0.4221 0.8031 0.0015 0.3875 1.6796 0.0030 0.8104 

 4040 552 2.1016 0.0038 0.9883 0.7496 0.0014 0.3525 0.6718 0.0012 0.3159 1.4084 0.0026 0.6623 

 4041 530 2.0509 0.0039 0.9748 0.7575 0.0014 0.3600 0.7721 0.0015 0.3670 1.5301 0.0029 0.7273 

 4042 595 1.6667 0.0028 0.7733 0.6949 0.0012 0.3224 0.7218 0.0012 0.3349 1.3973 0.0023 0.6483 

 4043 521 2.0053 0.0038 0.9531 0.7071 0.0014 0.3361 0.7201 0.0014 0.3423 1.4236 0.0027 0.6766 

 4044 541 2.2020 0.0041 1.0420 0.6748 0.0012 0.3193 0.6763 0.0013 0.3200 1.3432 0.0025 0.6356 

 4045 566 2.3618 0.0042 1.1085 0.8331 0.0015 0.3910 0.7462 0.0013 0.3502 1.5759 0.0028 0.7397 

 4046 578 1.8491 0.0032 0.8098 0.7788 0.0013 0.3411 0.8385 0.0015 0.3672 1.5720 0.0027 0.6884 

 4047 608 2.3012 0.0038 1.1094 0.7390 0.0012 0.3563 0.7661 0.0013 0.3693 1.5110 0.0025 0.7285 

 4048 655 2.5843 0.0039 1.0990 0.7845 0.0012 0.3336 0.7874 0.0012 0.3349 1.5650 0.0024 0.6655 

 4049 534 1.9375 0.0036 0.9245 0.6646 0.0012 0.3171 0.6540 0.0012 0.3121 1.3228 0.0025 0.6312 

 4050 463 1.9974 0.0043 0.9736 0.6817 0.0015 0.3323 0.6496 0.0014 0.3166 1.3338 0.0029 0.6502 

 4051 551 2.1462 0.0039 0.9879 0.7516 0.0014 0.3460 0.7424 0.0013 0.3417 1.4740 0.0027 0.6785 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 471 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 596 0.0497 0.0001 0.0225 2.2042 0.0037 1.0000 4.5914 0.0077 2.0830 21.6371 0.0363 9.8163 

 1027 595 0.0672 0.0001 0.0294 2.2881 0.0038 1.0000 4.3794 0.0074 1.9140 21.0723 0.0354 9.2095 

 1028 554 0.0622 0.0001 0.0275 2.2605 0.0041 1.0000 4.0830 0.0074 1.8062 19.5191 0.0352 8.6349 

 1029 522 0.0627 0.0001 0.0304 2.0606 0.0039 1.0000 3.5516 0.0068 1.7236 18.0607 0.0346 8.7648 

 1030 574 0.0607 0.0001 0.0271 2.2390 0.0039 1.0000 3.5564 0.0062 1.5884 19.1037 0.0333 8.5322 

 1031 547 0.0550 0.0001 0.0264 2.0844 0.0038 1.0000 3.9014 0.0071 1.8717 18.0676 0.0330 8.6680 

 1032 621 0.0563 0.0001 0.0239 2.3564 0.0038 1.0000 4.4814 0.0072 1.9018 23.1030 0.0372 9.8044 

 1033 561 0.0622 0.0001 0.0266 2.3383 0.0042 1.0000 4.2546 0.0076 1.8195 19.0090 0.0339 8.1294 

 1034 512 0.0540 0.0001 0.0264 2.0464 0.0040 1.0000 3.4090 0.0067 1.6659 16.0564 0.0314 7.8462 

 1035 535 0.0605 0.0001 0.0293 2.0642 0.0039 1.0000 3.3152 0.0062 1.6060 18.0211 0.0337 8.7303 

 1036 597 0.0585 0.0001 0.0262 2.2320 0.0037 1.0000 4.1916 0.0070 1.8780 19.2672 0.0323 8.6323 

 1037 522 0.0697 0.0001 0.0333 2.0959 0.0040 1.0000 3.5059 0.0067 1.6727 16.6953 0.0320 7.9657 

 1038 582 0.0655 0.0001 0.0315 2.0824 0.0036 1.0000 4.5715 0.0079 2.1953 20.3051 0.0349 9.7508 

 1039 510 0.0571 0.0001 0.0272 2.1010 0.0041 1.0000 3.7101 0.0073 1.7659 16.1684 0.0317 7.6956 

 1040 661 0.0552 0.0001 0.0246 2.2407 0.0034 1.0000 4.5382 0.0069 2.0253 21.8379 0.0330 9.7460 

 1041 683 0.0661 0.0001 0.0302 2.1881 0.0032 1.0000 4.9300 0.0072 2.2531 23.3785 0.0342 10.6844 

 1042 687 0.0650 0.0001 0.0286 2.2709 0.0033 1.0000 5.4274 0.0079 2.3900 23.0370 0.0335 10.1444 

 1043 445 0.0616 0.0001 0.0284 2.1687 0.0049 1.0000 3.4595 0.0078 1.5952 14.5009 0.0326 6.6864 

 1044 635 0.0788 0.0001 0.0384 2.0529 0.0032 1.0000 4.0483 0.0064 1.9720 22.4535 0.0354 10.9375 

 1045 555 0.0651 0.0001 0.0324 2.0099 0.0036 1.0000 3.7685 0.0068 1.8750 19.0286 0.0343 9.4674 

 1046 594 0.0672 0.0001 0.0298 2.2533 0.0038 1.0000 4.3610 0.0073 1.9354 20.1033 0.0338 8.9217 

 1047 530 0.0688 0.0001 0.0329 2.0935 0.0040 1.0000 3.8376 0.0072 1.8331 17.8346 0.0337 8.5190 

 1048 625 0.0803 0.0001 0.0355 2.2600 0.0036 1.0000 4.4123 0.0071 1.9523 18.8077 0.0301 8.3220 

 1049 605 0.0520 0.0001 0.0240 2.1668 0.0036 1.0000 4.2264 0.0070 1.9505 19.8124 0.0328 9.1436 

 1050 597 0.0608 0.0001 0.0258 2.3558 0.0039 1.0000 4.6326 0.0078 1.9665 19.6467 0.0329 8.3397 

 1051 505 0.0800 0.0002 0.0397 2.0152 0.0040 1.0000 3.7748 0.0075 1.8732 16.2320 0.0321 8.0548 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 472 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 505 0.0506 0.0001 0.0232 2.1777 0.0043 1.0000 3.7375 0.0074 1.7163 18.1728 0.0360 8.3450 

 2027 511 0.0551 0.0001 0.0241 2.2892 0.0045 1.0000 3.4106 0.0067 1.4899 16.4422 0.0322 7.1825 

 2028 567 0.0509 0.0001 0.0235 2.1690 0.0038 1.0000 4.1151 0.0073 1.8972 19.3291 0.0341 8.9115 

 2029 588 0.0491 0.0001 0.0231 2.1245 0.0036 1.0000 4.1381 0.0070 1.9478 19.5875 0.0333 9.2198 

 2030 625 0.0649 0.0001 0.0286 2.2677 0.0036 1.0000 4.7805 0.0076 2.1081 22.7354 0.0364 10.0258 

 2031 516 0.0753 0.0001 0.0355 2.1205 0.0041 1.0000 4.0010 0.0078 1.8868 18.1236 0.0351 8.5469 

 2032 492 0.0524 0.0001 0.0249 2.1011 0.0043 1.0000 3.3367 0.0068 1.5881 16.3219 0.0332 7.7683 

 2033 639 0.0654 0.0001 0.0296 2.2124 0.0035 1.0000 4.2193 0.0066 1.9071 20.8139 0.0326 9.4078 

 2034 608 0.0523 0.0001 0.0244 2.1419 0.0035 1.0000 4.2481 0.0070 1.9833 19.3562 0.0318 9.0369 

 2035 504 0.0518 0.0001 0.0256 2.0265 0.0040 1.0000 3.4495 0.0068 1.7022 15.4040 0.0306 7.6013 

 2036 491 0.0674 0.0001 0.0294 2.2892 0.0047 1.0000 3.9796 0.0081 1.7384 17.3032 0.0352 7.5586 

 2037 572 0.0690 0.0001 0.0308 2.2390 0.0039 1.0000 3.7521 0.0066 1.6758 18.1371 0.0317 8.1005 

 2038 540 0.0548 0.0001 0.0254 2.1588 0.0040 1.0000 4.5410 0.0084 2.1035 19.5089 0.0361 9.0369 

 2039 582 0.0721 0.0001 0.0329 2.1902 0.0038 1.0000 4.2684 0.0073 1.9489 19.8577 0.0341 9.0666 

 2040 581 0.0670 0.0001 0.0319 2.0994 0.0036 1.0000 4.3133 0.0074 2.0545 19.6690 0.0339 9.3689 

 2041 614 0.0611 0.0001 0.0284 2.1539 0.0035 1.0000 4.0158 0.0065 1.8644 20.4904 0.0334 9.5132 

 2042 551 0.0860 0.0002 0.0415 2.0726 0.0038 1.0000 3.5861 0.0065 1.7302 18.8070 0.0342 9.0741 

 2043 640 0.0856 0.0001 0.0388 2.2056 0.0034 1.0000 4.1182 0.0064 1.8672 23.0512 0.0360 10.4512 

 2044 674 0.0567 0.0001 0.0266 2.1349 0.0032 1.0000 4.3578 0.0065 2.0412 23.4208 0.0348 10.9704 

 2045 664 0.0773 0.0001 0.0363 2.1315 0.0032 1.0000 4.4919 0.0068 2.1074 22.8447 0.0344 10.7177 

 2046 563 0.0678 0.0001 0.0322 2.1039 0.0037 1.0000 4.1426 0.0074 1.9690 18.7236 0.0333 8.8995 

 2047 598 0.0770 0.0001 0.0364 2.1164 0.0035 1.0000 4.0237 0.0067 1.9012 20.0965 0.0336 9.4956 

 2048 495 0.0676 0.0001 0.0316 2.1367 0.0043 1.0000 3.8509 0.0078 1.8023 16.9682 0.0343 7.9413 

 2049 697 0.0871 0.0001 0.0387 2.2483 0.0032 1.0000 4.7940 0.0069 2.1323 24.4855 0.0352 10.8907 

 2050 606 0.0576 0.0001 0.0262 2.1959 0.0036 1.0000 4.2303 0.0070 1.9265 19.9556 0.0329 9.0877 

 2051 545 0.0575 0.0001 0.0264 2.1775 0.0040 1.0000 3.7397 0.0069 1.7174 18.7255 0.0343 8.5995 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 473 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 575 0.0570 0.0001 0.0271 2.1061 0.0037 1.0000 4.6270 0.0080 2.1970 20.5960 0.0358 9.7792 

 3027 485 0.0921 0.0002 0.0458 2.0101 0.0041 1.0000 3.9876 0.0082 1.9838 17.0162 0.0351 8.4654 

 3028 625 0.0625 0.0001 0.0278 2.2461 0.0036 1.0000 4.6372 0.0074 2.0646 20.0811 0.0321 8.9404 

 3029 611 0.0625 0.0001 0.0289 2.1637 0.0035 1.0000 4.6139 0.0076 2.1324 21.0988 0.0345 9.7513 

 3030 627 0.0767 0.0001 0.0352 2.1804 0.0035 1.0000 5.3011 0.0085 2.4313 22.4215 0.0357 10.2832 

 3031 524 0.0580 0.0001 0.0279 2.0770 0.0040 1.0000 3.8281 0.0073 1.8431 17.1023 0.0326 8.2341 

 3032 510 0.0647 0.0001 0.0301 2.1478 0.0042 1.0000 3.8597 0.0076 1.7970 15.5536 0.0305 7.2416 

 3033 587 0.0696 0.0001 0.0329 2.1164 0.0036 1.0000 4.4308 0.0075 2.0936 21.1561 0.0360 9.9963 

 3034 594 0.0581 0.0001 0.0256 2.2717 0.0038 1.0000 4.4644 0.0075 1.9652 19.8822 0.0335 8.7521 

 3035 624 0.0577 0.0001 0.0250 2.3123 0.0037 1.0000 4.9814 0.0080 2.1543 23.3690 0.0374 10.1064 

 3036 505 0.0523 0.0001 0.0247 2.1135 0.0042 1.0000 4.0199 0.0080 1.9020 16.8945 0.0334 7.9936 

 3037 577 0.0595 0.0001 0.0273 2.1817 0.0038 1.0000 4.5452 0.0079 2.0833 21.1925 0.0367 9.7138 

 3038 569 0.0795 0.0001 0.0362 2.1962 0.0039 1.0000 5.0869 0.0089 2.3162 20.5248 0.0361 9.3456 

 3039 568 0.0642 0.0001 0.0292 2.1968 0.0039 1.0000 4.8341 0.0085 2.2005 22.2637 0.0392 10.1346 

 3040 660 0.0684 0.0001 0.0319 2.1435 0.0033 1.0000 5.2329 0.0079 2.4413 23.7361 0.0360 11.0735 

 3041 629 0.0748 0.0001 0.0348 2.1504 0.0034 1.0000 4.9062 0.0078 2.2815 23.3730 0.0372 10.8691 

 3042 553 0.0590 0.0001 0.0278 2.1226 0.0038 1.0000 4.8675 0.0088 2.2932 17.7070 0.0320 8.3421 

 3045 476 0.0610 0.0001 0.0275 2.2180 0.0047 1.0000 3.6753 0.0077 1.6570 15.9964 0.0336 7.2121 

 3046 536 0.0840 0.0002 0.0389 2.1610 0.0040 1.0000 4.4532 0.0083 2.0607 18.1863 0.0339 8.4157 

 3047 559 0.0714 0.0001 0.0343 2.0816 0.0037 1.0000 5.0694 0.0091 2.4353 23.6349 0.0423 11.3542 

 3048 559 0.0593 0.0001 0.0282 2.1037 0.0038 1.0000 4.3111 0.0077 2.0493 19.7936 0.0354 9.4089 

 3049 550 0.0679 0.0001 0.0305 2.2276 0.0041 1.0000 4.5017 0.0082 2.0209 19.8437 0.0361 8.9081 

 3050 660 0.1011 0.0002 0.0425 2.3791 0.0036 1.0000 5.3506 0.0081 2.2490 24.8219 0.0376 10.4333 

 3051 646 0.0716 0.0001 0.0328 2.1816 0.0034 1.0000 4.8972 0.0076 2.2448 21.3150 0.0330 9.7704 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 474 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 642 0.0481 0.0001 0.0227 2.1219 0.0033 1.0000 4.9232 0.0077 2.3202 25.1112 0.0391 11.8343 

 4027 512 0.0529 0.0001 0.0242 2.1825 0.0043 1.0000 4.5250 0.0088 2.0733 18.7483 0.0366 8.5903 

 4028 597 0.0626 0.0001 0.0298 2.0995 0.0035 1.0000 4.5135 0.0076 2.1498 22.0398 0.0369 10.4976 

 4029 657 0.0602 0.0001 0.0269 2.2364 0.0034 1.0000 6.1342 0.0093 2.7429 23.0784 0.0351 10.3194 

 4030 591 0.0628 0.0001 0.0288 2.1803 0.0037 1.0000 4.8956 0.0083 2.2454 21.8050 0.0369 10.0009 

 4031 504 0.0593 0.0001 0.0268 2.2086 0.0044 1.0000 5.0211 0.0100 2.2734 20.4573 0.0406 9.2626 

 4032 783 0.0695 0.0001 0.0326 2.1325 0.0027 1.0000 5.8810 0.0075 2.7578 29.3185 0.0374 13.7484 

 4033 530 0.0608 0.0001 0.0291 2.0923 0.0039 1.0000 5.3645 0.0101 2.5639 20.4241 0.0385 9.7616 

 4034 553 0.0443 0.0001 0.0221 2.0068 0.0036 1.0000 4.0653 0.0074 2.0258 20.1266 0.0364 10.0292 

 4035 498 0.0685 0.0001 0.0343 1.9996 0.0040 1.0000 4.8185 0.0097 2.4097 19.0169 0.0382 9.5104 

 4036 596 0.0574 0.0001 0.0260 2.2083 0.0037 1.0000 4.1563 0.0070 1.8821 21.2134 0.0356 9.6062 

 4037 550 0.0463 0.0001 0.0227 2.0358 0.0037 1.0000 5.0785 0.0092 2.4946 20.5777 0.0374 10.1079 

 4038 520 0.0612 0.0001 0.0299 2.0443 0.0039 1.0000 4.5094 0.0087 2.2058 18.7277 0.0360 9.1609 

 4039 552 0.0670 0.0001 0.0323 2.0725 0.0038 1.0000 4.5545 0.0083 2.1976 19.8811 0.0360 9.5928 

 4040 552 0.0743 0.0001 0.0349 2.1265 0.0039 1.0000 4.4954 0.0082 2.1140 23.6899 0.0430 11.1403 

 4041 530 0.0498 0.0001 0.0237 2.1039 0.0040 1.0000 4.9329 0.0093 2.3446 18.7611 0.0354 8.9173 

 4042 595 0.0571 0.0001 0.0265 2.1554 0.0036 1.0000 4.5598 0.0077 2.1155 23.7247 0.0399 11.0071 

 4043 521 0.0486 0.0001 0.0231 2.1039 0.0040 1.0000 4.1860 0.0080 1.9896 18.8542 0.0362 8.9615 

 4044 541 0.0675 0.0001 0.0319 2.1132 0.0039 1.0000 4.8299 0.0089 2.2856 21.7772 0.0403 10.3053 

 4045 566 0.0605 0.0001 0.0284 2.1306 0.0038 1.0000 4.6227 0.0082 2.1697 20.3016 0.0359 9.5286 

 4046 578 0.0590 0.0001 0.0258 2.2835 0.0040 1.0000 5.1649 0.0089 2.2618 20.9115 0.0362 9.1577 

 4047 608 0.0808 0.0001 0.0390 2.0742 0.0034 1.0000 4.9660 0.0082 2.3942 22.3447 0.0368 10.7727 

 4048 655 0.0913 0.0001 0.0388 2.3515 0.0036 1.0000 4.3992 0.0067 1.8708 23.8447 0.0364 10.1402 

 4049 534 0.0568 0.0001 0.0271 2.0957 0.0039 1.0000 4.3855 0.0082 2.0926 20.3720 0.0381 9.7209 

 4050 463 0.0577 0.0001 0.0281 2.0515 0.0044 1.0000 3.6937 0.0080 1.8005 17.0722 0.0368 8.3218 

 4051 551 0.0541 0.0001 0.0249 2.1725 0.0039 1.0000 4.1878 0.0076 1.9276 19.6771 0.0357 9.0574 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 475 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1026 596 1.6649 0.0028 0.7553 0.0125 0.0000 0.0057 0.7026 0.0012 0.3188 

 1027 595 1.6803 0.0028 0.7344 0.0128 0.0000 0.0056 0.7819 0.0013 0.3417 

 1028 554 1.6226 0.0029 0.7178 0.0110 0.0000 0.0049 0.8475 0.0015 0.3749 

 1029 522 1.7016 0.0033 0.8258 0.0107 0.0000 0.0052 0.7528 0.0014 0.3653 

 1030 574 1.8358 0.0032 0.8199 0.0121 0.0000 0.0054 0.7566 0.0013 0.3379 

 1031 547 1.6697 0.0031 0.8010 0.0109 0.0000 0.0052 0.7167 0.0013 0.3438 

 1032 621 2.4779 0.0040 1.0516 0.0151 0.0000 0.0064 0.8986 0.0014 0.3813 

 1033 561 1.7288 0.0031 0.7393 0.0111 0.0000 0.0047 0.7179 0.0013 0.3070 

 1034 512 1.5607 0.0031 0.7627 0.0117 0.0000 0.0057 0.7868 0.0015 0.3845 

 1035 535 1.5183 0.0028 0.7355 0.0090 0.0000 0.0044 0.7235 0.0014 0.3505 

 1036 597 2.0919 0.0035 0.9372 0.0104 0.0000 0.0047 0.8550 0.0014 0.3831 

 1037 522 1.5740 0.0030 0.7510 0.0108 0.0000 0.0052 0.7675 0.0015 0.3662 

 1038 582 2.3818 0.0041 1.1438 0.0131 0.0000 0.0063 1.0208 0.0018 0.4902 

 1039 510 1.5953 0.0031 0.7593 0.0103 0.0000 0.0049 0.7961 0.0016 0.3789 

 1040 661 2.0305 0.0031 0.9062 0.0131 0.0000 0.0058 0.9771 0.0015 0.4361 

 1041 683 2.2176 0.0032 1.0135 0.0131 0.0000 0.0060 0.9468 0.0014 0.4327 

 1042 687 1.9694 0.0029 0.8672 0.0122 0.0000 0.0054 0.9194 0.0013 0.4049 

 1043 445 1.7349 0.0039 0.8000 0.0100 0.0000 0.0046 0.6989 0.0016 0.3223 

 1044 635 1.7016 0.0027 0.8289 0.0112 0.0000 0.0055 1.0277 0.0016 0.5006 

 1045 555 1.6095 0.0029 0.8008 0.0139 0.0000 0.0069 0.6809 0.0012 0.3388 

 1046 594 1.8767 0.0032 0.8329 0.0127 0.0000 0.0056 0.5907 0.0010 0.2621 

 1047 530 1.7311 0.0033 0.8269 0.0123 0.0000 0.0059 0.7426 0.0014 0.3547 

 1048 625 1.8679 0.0030 0.8265 0.0145 0.0000 0.0064 1.2141 0.0019 0.5372 

 1049 605 1.9854 0.0033 0.9163 0.0109 0.0000 0.0050 0.7879 0.0013 0.3636 

 1050 597 2.2006 0.0037 0.9341 0.0137 0.0000 0.0058 0.6994 0.0012 0.2969 

 1051 505 1.9452 0.0038 0.9653 0.0117 0.0000 0.0058 0.7669 0.0015 0.3806 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 476 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2026 505 1.6806 0.0033 0.7717 0.0066 0.0000 0.0030 0.7052 0.0014 0.3238 

 2027 511 1.4735 0.0029 0.6437 0.0103 0.0000 0.0045 0.6373 0.0012 0.2784 

 2028 567 1.6954 0.0030 0.7817 0.0055 0.0000 0.0025 0.8434 0.0015 0.3888 

 2029 588 1.9105 0.0033 0.8993 0.0130 0.0000 0.0061 0.8114 0.0014 0.3819 

 2030 625 2.0506 0.0033 0.9043 0.0116 0.0000 0.0051 0.9337 0.0015 0.4117 

 2031 516 1.5817 0.0031 0.7459 0.0109 0.0000 0.0051 0.7684 0.0015 0.3624 

 2032 492 1.5945 0.0032 0.7589 0.0087 0.0000 0.0041 0.7208 0.0015 0.3431 

 2033 639 1.9385 0.0030 0.8762 0.0115 0.0000 0.0052 0.7357 0.0012 0.3325 

 2034 608 1.7966 0.0030 0.8388 0.0125 0.0000 0.0058 0.9700 0.0016 0.4529 

 2035 504 1.5852 0.0031 0.7822 0.0127 0.0000 0.0063 0.6600 0.0013 0.3257 

 2036 491 1.9539 0.0040 0.8535 0.0118 0.0000 0.0052 0.7581 0.0015 0.3312 

 2037 572 1.8430 0.0032 0.8231 0.0117 0.0000 0.0052 0.6169 0.0011 0.2755 

 2038 540 1.5982 0.0030 0.7403 0.0101 0.0000 0.0047 0.9692 0.0018 0.4490 

 2039 582 1.8116 0.0031 0.8271 0.0103 0.0000 0.0047 0.6941 0.0012 0.3169 

 2040 581 1.8326 0.0032 0.8729 0.0123 0.0000 0.0059 0.7065 0.0012 0.3365 

 2041 614 1.8902 0.0031 0.8776 0.0109 0.0000 0.0051 0.7968 0.0013 0.3699 

 2042 551 1.7207 0.0031 0.8302 0.0108 0.0000 0.0052 0.6609 0.0012 0.3189 

 2043 640 2.0147 0.0031 0.9134 0.0112 0.0000 0.0051 0.8252 0.0013 0.3741 

 2044 674 1.8177 0.0027 0.8514 0.0121 0.0000 0.0057 0.6028 0.0009 0.2824 

 2045 664 1.8537 0.0028 0.8697 0.0138 0.0000 0.0065 0.7125 0.0011 0.3343 

 2046 563 1.9157 0.0034 0.9105 0.0121 0.0000 0.0058 0.8378 0.0015 0.3982 

 2047 598 1.7662 0.0030 0.8345 0.0112 0.0000 0.0053 0.8314 0.0014 0.3928 

 2048 495 1.7010 0.0034 0.7961 0.0131 0.0000 0.0061 0.9375 0.0019 0.4388 

 2049 697 2.3427 0.0034 1.0420 0.0152 0.0000 0.0068 0.7549 0.0011 0.3358 

 2050 606 1.7826 0.0029 0.8118 0.0139 0.0000 0.0063 0.7247 0.0012 0.3300 

 2051 545 1.9435 0.0036 0.8925 0.0120 0.0000 0.0055 0.7855 0.0014 0.3607 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 477 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 3026 575 1.6761 0.0029 0.7958 0.0105 0.0000 0.0050 0.7205 0.0013 0.3421 

 3027 485 1.5179 0.0031 0.7551 0.0107 0.0000 0.0053 0.7227 0.0015 0.3595 

 3028 625 2.1264 0.0034 0.9467 0.0121 0.0000 0.0054 0.6807 0.0011 0.3031 

 3029 611 1.7633 0.0029 0.8149 0.0119 0.0000 0.0055 0.7797 0.0013 0.3604 

 3030 627 2.0062 0.0032 0.9201 0.0121 0.0000 0.0055 1.0044 0.0016 0.4606 

 3031 524 1.6220 0.0031 0.7809 0.0122 0.0000 0.0059 0.7442 0.0014 0.3583 

 3032 510 1.6470 0.0032 0.7668 0.0134 0.0000 0.0062 0.8738 0.0017 0.4068 

 3033 587 2.2473 0.0038 1.0619 0.0115 0.0000 0.0054 0.8757 0.0015 0.4138 

 3034 594 1.7066 0.0029 0.7512 0.0127 0.0000 0.0056 0.7063 0.0012 0.3109 

 3035 624 1.9884 0.0032 0.8599 0.0106 0.0000 0.0046 0.7755 0.0012 0.3354 

 3036 505 1.6280 0.0032 0.7703 0.0094 0.0000 0.0044 0.7708 0.0015 0.3647 

 3037 577 1.8304 0.0032 0.8390 0.0110 0.0000 0.0050 0.8814 0.0015 0.4040 

 3038 569 2.2100 0.0039 1.0063 0.0123 0.0000 0.0056 0.8817 0.0015 0.4015 

 3039 568 1.7922 0.0032 0.8158 0.0123 0.0000 0.0056 0.9555 0.0017 0.4350 

 3040 660 1.8278 0.0028 0.8527 0.0115 0.0000 0.0054 1.0728 0.0016 0.5005 

 3041 629 1.7134 0.0027 0.7968 0.0139 0.0000 0.0065 1.0363 0.0016 0.4819 

 3042 553 1.8185 0.0033 0.8567 0.0107 0.0000 0.0050 0.7856 0.0014 0.3701 

 3045 476 1.7899 0.0038 0.8070 0.0105 0.0000 0.0047 0.5933 0.0012 0.2675 

 3046 536 1.8109 0.0034 0.8380 0.0116 0.0000 0.0054 0.8835 0.0016 0.4088 

 3047 559 1.8809 0.0034 0.9036 0.0113 0.0000 0.0054 0.8246 0.0015 0.3961 

 3048 559 1.7809 0.0032 0.8466 0.0124 0.0000 0.0059 0.9210 0.0016 0.4378 

 3049 550 1.9036 0.0035 0.8546 0.0130 0.0000 0.0058 0.8421 0.0015 0.3780 

 3050 660 2.2453 0.0034 0.9438 0.0145 0.0000 0.0061 1.0414 0.0016 0.4377 

 3051 646 2.2972 0.0036 1.0530 0.0149 0.0000 0.0068 0.7781 0.0012 0.3567 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 478 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

MALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4026 642 2.0399 0.0032 0.9614 0.0118 0.0000 0.0056 0.8034 0.0013 0.3786 

 4027 512 1.7243 0.0034 0.7901 0.0123 0.0000 0.0056 0.6395 0.0012 0.2930 

 4028 597 1.6918 0.0028 0.8058 0.0107 0.0000 0.0051 0.7258 0.0012 0.3457 

 4029 657 1.9865 0.0030 0.8883 0.0097 0.0000 0.0043 0.7905 0.0012 0.3535 

 4030 591 1.5825 0.0027 0.7258 0.0140 0.0000 0.0064 0.8130 0.0014 0.3729 

 4031 504 2.0731 0.0041 0.9386 0.0160 0.0000 0.0072 0.8205 0.0016 0.3715 

 4032 783 2.0094 0.0026 0.9423 0.0108 0.0000 0.0051 1.1075 0.0014 0.5193 

 4033 530 1.7040 0.0032 0.8144 0.0119 0.0000 0.0057 0.7246 0.0014 0.3463 

 4034 553 1.8835 0.0034 0.9386 0.0109 0.0000 0.0054 0.7353 0.0013 0.3664 

 4035 498 1.7434 0.0035 0.8719 0.0119 0.0000 0.0060 0.7869 0.0016 0.3935 

 4036 596 1.8743 0.0031 0.8488 0.0127 0.0000 0.0058 0.8058 0.0014 0.3649 

 4037 550 1.8141 0.0033 0.8911 0.0070 0.0000 0.0034 0.8321 0.0015 0.4087 

 4038 520 1.7147 0.0033 0.8388 0.0124 0.0000 0.0061 0.8396 0.0016 0.4107 

 4039 552 1.8113 0.0033 0.8740 0.0105 0.0000 0.0051 0.6913 0.0013 0.3336 

 4040 552 1.9324 0.0035 0.9087 0.0126 0.0000 0.0059 0.7671 0.0014 0.3607 

 4041 530 1.6449 0.0031 0.7818 0.0118 0.0000 0.0056 0.8959 0.0017 0.4258 

 4042 595 1.7210 0.0029 0.7985 0.0090 0.0000 0.0042 0.7000 0.0012 0.3248 

 4043 521 1.6643 0.0032 0.7911 0.0083 0.0000 0.0039 0.6125 0.0012 0.2911 

 4044 541 1.9595 0.0036 0.9273 0.0094 0.0000 0.0044 0.8839 0.0016 0.4183 

 4045 566 2.0707 0.0037 0.9719 0.0136 0.0000 0.0064 0.8277 0.0015 0.3885 

 4046 578 1.7913 0.0031 0.7845 0.0123 0.0000 0.0054 0.7890 0.0014 0.3455 

 4047 608 1.8242 0.0030 0.8795 0.0148 0.0000 0.0071 0.6940 0.0011 0.3346 

 4048 655 2.0407 0.0031 0.8678 0.0145 0.0000 0.0062 0.9682 0.0015 0.4117 

 4049 534 1.8180 0.0034 0.8675 0.0133 0.0000 0.0063 0.7544 0.0014 0.3600 

 4050 463 1.4950 0.0032 0.7287 0.0110 0.0000 0.0054 0.6562 0.0014 0.3199 

 4051 551 1.7979 0.0033 0.8276 0.0131 0.0000 0.0060 0.8929 0.0016 0.4110 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 479 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 268 0.0690 0.0003 0.0357 1.9342 0.0072 1.0000 2.2961 0.0086 1.1871 11.7388 0.0438 6.0691 

 1527 306 0.0656 0.0002 0.0353 1.8588 0.0061 1.0000 2.7035 0.0088 1.4544 14.5085 0.0475 7.8053 

 1528 269 0.0709 0.0003 0.0360 1.9697 0.0073 1.0000 2.2505 0.0084 1.1426 11.3636 0.0423 5.7692 

 1529 309 0.0720 0.0002 0.0358 2.0096 0.0065 1.0000 2.3786 0.0077 1.1836 12.8991 0.0417 6.4187 

 1530 327 0.0685 0.0002 0.0356 1.9232 0.0059 1.0000 2.5788 0.0079 1.3409 13.7154 0.0419 7.1316 

 1531 295 0.0675 0.0002 0.0359 1.8810 0.0064 1.0000 2.2593 0.0076 1.2011 12.7914 0.0433 6.8003 

 1532 316 0.0675 0.0002 0.0358 1.8852 0.0060 1.0000 2.8307 0.0090 1.5015 14.9345 0.0473 7.9220 

 1533 329 0.0709 0.0002 0.0350 2.0271 0.0062 1.0000 2.7475 0.0083 1.3554 14.0334 0.0426 6.9229 

 1535 311 0.0693 0.0002 0.0358 1.9345 0.0062 1.0000 2.5821 0.0083 1.3348 14.2344 0.0458 7.3582 

 1536 331 0.0738 0.0002 0.0366 2.0152 0.0061 1.0000 2.6592 0.0080 1.3196 13.8851 0.0419 6.8902 

 1537 342 0.0746 0.0002 0.0368 2.0276 0.0059 1.0000 2.6700 0.0078 1.3168 16.7770 0.0491 8.2743 

 1539 339 0.0887 0.0003 0.0446 1.9881 0.0059 1.0000 2.7608 0.0081 1.3887 16.8518 0.0497 8.4763 

 1541 335 0.0839 0.0003 0.0412 2.0364 0.0061 1.0000 2.7186 0.0081 1.3350 16.4748 0.0493 8.0902 

 1542 300 0.0802 0.0003 0.0433 1.8543 0.0062 1.0000 2.7735 0.0092 1.4957 14.5041 0.0483 7.8219 

 1543 311 0.0947 0.0003 0.0536 1.7676 0.0057 1.0000 2.5818 0.0083 1.4606 13.3808 0.0431 7.5700 

 1544 344 0.0773 0.0002 0.0401 1.9268 0.0056 1.0000 2.7489 0.0080 1.4267 16.2738 0.0473 8.4460 

 1545 284 0.0673 0.0002 0.0344 1.9579 0.0069 1.0000 2.4160 0.0085 1.2340 13.3421 0.0469 6.8145 

 1546 304 0.0678 0.0002 0.0340 1.9927 0.0066 1.0000 2.6363 0.0087 1.3230 14.1209 0.0464 7.0863 

 1547 291 0.0669 0.0002 0.0346 1.9363 0.0066 1.0000 2.3435 0.0080 1.2103 12.3125 0.0423 6.3588 

 1550 268 0.0736 0.0003 0.0364 2.0198 0.0075 1.0000 2.4009 0.0090 1.1887 11.2886 0.0422 5.5890 

 1551 292 0.0706 0.0002 0.0394 1.7897 0.0061 1.0000 2.5342 0.0087 1.4160 12.8911 0.0441 7.2029 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 480 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 303 0.0983 0.0003 0.0521 1.8875 0.0062 1.0000 2.4309 0.0080 1.2879 12.5073 0.0412 6.6264 

 2527 291 0.0848 0.0003 0.0448 1.8927 0.0065 1.0000 2.4557 0.0085 1.2975 12.5833 0.0433 6.6483 

 2528 330 0.0909 0.0003 0.0460 1.9754 0.0060 1.0000 2.9181 0.0088 1.4772 15.1089 0.0458 7.6485 

 2529 292 0.0681 0.0002 0.0360 1.8901 0.0065 1.0000 2.5470 0.0087 1.3475 12.4484 0.0427 6.5861 

 2530 291 0.0795 0.0003 0.0417 1.9076 0.0066 1.0000 2.5260 0.0087 1.3242 12.9113 0.0444 6.7683 

 2531 321 0.0723 0.0002 0.0378 1.9146 0.0060 1.0000 2.6537 0.0083 1.3860 14.8639 0.0462 7.7634 

 2532 309 0.0616 0.0002 0.0345 1.7866 0.0058 1.0000 2.6984 0.0087 1.5104 13.5693 0.0439 7.5950 

 2533 366 0.0724 0.0002 0.0353 2.0527 0.0056 1.0000 2.9310 0.0080 1.4279 16.4653 0.0450 8.0213 

 2534 326 0.0772 0.0002 0.0411 1.8774 0.0058 1.0000 2.6450 0.0081 1.4089 14.1727 0.0435 7.5491 

 2535 299 0.0619 0.0002 0.0364 1.7014 0.0057 1.0000 2.2113 0.0074 1.2997 12.8387 0.0430 7.5460 

 2537 348 0.0652 0.0002 0.0326 2.0006 0.0057 1.0000 2.7179 0.0078 1.3585 13.7327 0.0394 6.8643 

 2538 343 0.0783 0.0002 0.0381 2.0552 0.0060 1.0000 2.3884 0.0070 1.1621 12.9122 0.0376 6.2827 

 2539 315 0.0733 0.0002 0.0359 2.0419 0.0065 1.0000 2.5320 0.0080 1.2400 15.2936 0.0486 7.4899 

 2540 278 0.0697 0.0003 0.0368 1.8950 0.0068 1.0000 2.5245 0.0091 1.3322 12.3091 0.0442 6.4956 

 2541 310 0.0668 0.0002 0.0347 1.9250 0.0062 1.0000 2.5505 0.0082 1.3249 13.8606 0.0447 7.2003 

 2542 298 0.0829 0.0003 0.0435 1.9049 0.0064 1.0000 2.6618 0.0089 1.3973 12.8159 0.0430 6.7279 

 2543 PNMD 322 0.0700 0.0002 0.0370 1.8915 0.0059 1.0000 2.6686 0.0083 1.4108 16.4246 0.0510 8.6834 

 2545 331 0.0711 0.0002 0.0367 1.9371 0.0059 1.0000 2.6877 0.0081 1.3875 14.0572 0.0425 7.2568 

 2546 314 0.1003 0.0003 0.0526 1.9068 0.0061 1.0000 2.6617 0.0085 1.3959 16.1563 0.0515 8.4730 

 2547 265 0.0784 0.0003 0.0388 2.0203 0.0076 1.0000 2.2106 0.0083 1.0942 9.0096 0.0339 4.4595 

 2548 308 0.0631 0.0002 0.0316 1.9982 0.0065 1.0000 2.5685 0.0083 1.2854 13.9637 0.0453 6.9881 

 2550 293 0.0604 0.0002 0.0341 1.7726 0.0060 1.0000 2.6035 0.0089 1.4687 13.9971 0.0477 7.8964 

 2551 327 0.0802 0.0002 0.0408 1.9680 0.0060 1.0000 2.8744 0.0088 1.4606 16.5279 0.0505 8.3983 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE 
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 481 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 303 0.1072 0.0004 0.0549 1.9514 0.0064 1.0000 2.4618 0.0081 1.2616 14.2889 0.0471 7.3224 

 3528 355 0.0871 0.0002 0.0434 2.0072 0.0057 1.0000 2.7306 0.0077 1.3604 14.6180 0.0412 7.2828 

 3529 287 0.0854 0.0003 0.0456 1.8720 0.0065 1.0000 2.5469 0.0089 1.3605 12.6058 0.0439 6.7339 

 3531 310 0.0863 0.0003 0.0458 1.8842 0.0061 1.0000 2.8295 0.0091 1.5017 15.5423 0.0501 8.2488 

 3532 297 0.0662 0.0002 0.0348 1.9042 0.0064 1.0000 2.4502 0.0083 1.2867 12.5695 0.0423 6.6009 

 3533 305 0.0806 0.0003 0.0409 1.9717 0.0065 1.0000 2.4181 0.0079 1.2264 13.7093 0.0449 6.9530 

 3535 275 0.0682 0.0002 0.0373 1.8270 0.0066 1.0000 2.5479 0.0093 1.3946 11.5774 0.0421 6.3368 

 3536 304 0.0698 0.0002 0.0345 2.0254 0.0067 1.0000 2.7019 0.0089 1.3340 15.0901 0.0496 7.4504 

 3537 288 0.0594 0.0002 0.0297 1.9994 0.0069 1.0000 2.6672 0.0093 1.3340 12.6084 0.0438 6.3061 

 3538 326 0.0700 0.0002 0.0363 1.9281 0.0059 1.0000 2.6460 0.0081 1.3723 16.3096 0.0501 8.4589 

 3539 343 0.0650 0.0002 0.0328 1.9800 0.0058 1.0000 2.7390 0.0080 1.3833 17.4218 0.0508 8.7989 

 3540 320 0.0540 0.0002 0.0276 1.9589 0.0061 1.0000 2.8679 0.0090 1.4640 16.6089 0.0519 8.4787 

 3541 339 0.0584 0.0002 0.0301 1.9415 0.0057 1.0000 2.5620 0.0076 1.3196 16.6451 0.0491 8.5733 

 3542 316 0.0736 0.0002 0.0380 1.9367 0.0061 1.0000 2.8326 0.0090 1.4626 13.4752 0.0426 6.9578 

 3543 291 0.0637 0.0002 0.0342 1.8631 0.0064 1.0000 2.3508 0.0081 1.2618 13.2622 0.0456 7.1184 

 3544 287 0.0615 0.0002 0.0351 1.7543 0.0061 1.0000 2.4389 0.0085 1.3902 12.9773 0.0453 7.3974 

 3545 336 0.0649 0.0002 0.0338 1.9219 0.0057 1.0000 3.0667 0.0091 1.5957 15.1961 0.0452 7.9068 

 3546 335 0.0590 0.0002 0.0306 1.9307 0.0058 1.0000 2.6194 0.0078 1.3567 14.3649 0.0429 7.4403 

 3547 275 0.0648 0.0002 0.0342 1.8942 0.0069 1.0000 2.2186 0.0081 1.1713 12.2604 0.0446 6.4726 

 3548 319 0.0736 0.0002 0.0382 1.9275 0.0060 1.0000 2.8445 0.0089 1.4757 14.4653 0.0454 7.5047 

 3549 289 0.0833 0.0003 0.0421 1.9794 0.0069 1.0000 2.6258 0.0091 1.3266 13.5990 0.0471 6.8703 

 3550 309 0.0944 0.0003 0.0481 1.9607 0.0064 1.0000 2.7409 0.0089 1.3979 15.0784 0.0489 7.6903 

 3551 318 0.0771 0.0002 0.0407 1.8955 0.0060 1.0000 2.7704 0.0087 1.4616 15.7650 0.0497 8.3171 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 482 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 ADRENALS BRAIN KIDNEYS LIVER 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 288 0.0824 0.0003 0.0415 1.9867 0.0069 1.0000 2.5238 0.0088 1.2703 13.0964 0.0456 6.5920 

 4527 306 0.0647 0.0002 0.0321 2.0171 0.0066 1.0000 2.4326 0.0080 1.2060 14.5006 0.0474 7.1888 

 4528 308 0.0809 0.0003 0.0398 2.0308 0.0066 1.0000 2.9558 0.0096 1.4555 12.7873 0.0416 6.2967 

 4529 353 0.1056 0.0003 0.0527 2.0034 0.0057 1.0000 3.1637 0.0090 1.5792 16.2706 0.0461 8.1215 

 4530 PNMD 307 0.0839 0.0003 0.0462 1.8168 0.0059 1.0000 2.8369 0.0092 1.5615 13.3414 0.0434 7.3434 

 4531 317 0.0824 0.0003 0.0419 1.9667 0.0062 1.0000 2.8388 0.0089 1.4434 12.9324 0.0408 6.5757 

 4532 300 0.0718 0.0002 0.0383 1.8751 0.0063 1.0000 2.4919 0.0083 1.3289 13.2965 0.0443 7.0911 

 4533 303 0.0704 0.0002 0.0367 1.9170 0.0063 1.0000 2.5105 0.0083 1.3096 14.5237 0.0480 7.5763 

 4534 300 0.0629 0.0002 0.0339 1.8569 0.0062 1.0000 2.8176 0.0094 1.5174 13.2892 0.0443 7.1567 

 4535 352 0.0694 0.0002 0.0340 2.0384 0.0058 1.0000 2.8218 0.0080 1.3843 18.1790 0.0517 8.9183 

 4536 345 0.0765 0.0002 0.0391 1.9558 0.0057 1.0000 2.8459 0.0082 1.4551 17.9329 0.0519 9.1691 

 4537 312 0.0813 0.0003 0.0430 1.8927 0.0061 1.0000 2.7381 0.0088 1.4467 14.6032 0.0468 7.7155 

 4539 300 0.0734 0.0002 0.0399 1.8382 0.0061 1.0000 2.6265 0.0087 1.4288 12.8644 0.0428 6.9984 

 4540 359 0.0800 0.0002 0.0388 2.0628 0.0057 1.0000 3.0311 0.0084 1.4694 18.2552 0.0508 8.8497 

 4541 290 0.0916 0.0003 0.0474 1.9328 0.0067 1.0000 2.5288 0.0087 1.3084 12.6979 0.0439 6.5697 

 4542 268 0.0675 0.0003 0.0360 1.8728 0.0070 1.0000 2.2435 0.0084 1.1979 12.3581 0.0462 6.5987 

 4545 293 0.0801 0.0003 0.0434 1.8446 0.0063 1.0000 2.5420 0.0087 1.3781 14.5665 0.0497 7.8968 

 4546 352 0.0690 0.0002 0.0359 1.9232 0.0055 1.0000 2.7978 0.0080 1.4548 16.7635 0.0477 8.7165 

 4547 365 0.0923 0.0003 0.0450 2.0506 0.0056 1.0000 3.1394 0.0086 1.5310 18.1232 0.0496 8.8380 

 4548 271 0.0680 0.0003 0.0357 1.9051 0.0070 1.0000 2.6210 0.0097 1.3758 10.9666 0.0405 5.7564 

 4549 280 0.0702 0.0003 0.0355 1.9768 0.0070 1.0000 2.4548 0.0088 1.2418 13.9113 0.0496 7.0373 

 4550 282 0.0627 0.0002 0.0329 1.9051 0.0068 1.0000 2.3135 0.0082 1.2144 13.8635 0.0492 7.2770 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE 
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 483 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN OVARY RIGHT 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 268 1.3146 0.0049 0.6797 0.0139 0.0001 0.0072 0.4915 0.0018 0.2541 0.0507 0.0002 0.0262 

 1527 306 1.2909 0.0042 0.6945 0.0121 0.0000 0.0065 0.6723 0.0022 0.3617 0.0329 0.0001 0.0177 

 1528 269 1.3974 0.0052 0.7094 0.0146 0.0001 0.0074 0.6331 0.0024 0.3214 0.0751 0.0003 0.0381 

 1529 309 1.4990 0.0048 0.7459 0.0129 0.0000 0.0064 0.4276 0.0014 0.2128 0.0670 0.0002 0.0333 

 1530 327 1.4945 0.0046 0.7771 0.0187 0.0001 0.0097 0.5916 0.0018 0.3076 0.0551 0.0002 0.0287 

 1531 295 1.4090 0.0048 0.7491 0.0153 0.0001 0.0081 0.4450 0.0015 0.2366 0.0612 0.0002 0.0325 

 1532 316 1.4956 0.0047 0.7933 0.0151 0.0000 0.0080 0.6370 0.0020 0.3379 0.0495 0.0002 0.0263 

 1533 329 1.5628 0.0047 0.7710 0.0178 0.0001 0.0088 0.5645 0.0017 0.2785 0.0590 0.0002 0.0291 

 1535 311 1.4794 0.0048 0.7647 0.0154 0.0000 0.0080 0.6440 0.0021 0.3329 0.0521 0.0002 0.0269 

 1536 331 1.4262 0.0043 0.7077 0.0156 0.0000 0.0077 0.7043 0.0021 0.3495 0.0515 0.0002 0.0256 

 1537 342 1.5461 0.0045 0.7625 0.0070 0.0000 0.0035 0.5333 0.0016 0.2630 0.0459 0.0001 0.0226 

 1539 339 1.6031 0.0047 0.8063 0.0137 0.0000 0.0069 0.6873 0.0020 0.3457 0.0785 0.0002 0.0395 

 1541 335 1.6498 0.0049 0.8102 0.0138 0.0000 0.0068 0.6265 0.0019 0.3077 0.0678 0.0002 0.0333 

 1542 300 1.6510 0.0055 0.8904 0.0153 0.0001 0.0083 0.6142 0.0020 0.3312 0.0695 0.0002 0.0375 

 1543 311 1.3524 0.0044 0.7651 0.0161 0.0001 0.0091 0.6290 0.0020 0.3558 0.0797 0.0003 0.0451 

 1544 344 1.5239 0.0044 0.7909 0.0175 0.0001 0.0091 0.6493 0.0019 0.3370 0.0578 0.0002 0.0300 

 1545 284 1.4049 0.0049 0.7176 0.0085 0.0000 0.0043 0.5663 0.0020 0.2892 0.0507 0.0002 0.0259 

 1546 304 1.3226 0.0043 0.6637 0.0144 0.0000 0.0072 0.5174 0.0017 0.2596 0.0587 0.0002 0.0295 

 1547 291 1.3949 0.0048 0.7204 0.0155 0.0001 0.0080 0.6403 0.0022 0.3307 0.0479 0.0002 0.0247 

 1550 268 1.4211 0.0053 0.7036 0.0141 0.0001 0.0070 0.4723 0.0018 0.2338 0.0543 0.0002 0.0269 

 1551 292 1.3189 0.0045 0.7369 0.0137 0.0000 0.0077 0.5777 0.0020 0.3228 0.0474 0.0002 0.0265 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 484 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN OVARY RIGHT 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 303 1.5215 0.0050 0.8061 0.0198 0.0001 0.0105 0.5793 0.0019 0.3069 0.0673 0.0002 0.0357 

 2527 291 1.5100 0.0052 0.7978 0.0172 0.0001 0.0091 0.5762 0.0020 0.3044 0.0703 0.0002 0.0371 

 2528 330 1.3997 0.0042 0.7086 0.0204 0.0001 0.0103 0.6536 0.0020 0.3309 0.0494 0.0001 0.0250 

 2529 292 1.4160 0.0049 0.7492 0.0161 0.0001 0.0085 0.5114 0.0018 0.2706 0.0409 0.0001 0.0216 

 2530 291 1.4907 0.0051 0.7815 0.0198 0.0001 0.0104 0.5916 0.0020 0.3101 0.0640 0.0002 0.0336 

 2531 321 1.3404 0.0042 0.7001 0.0164 0.0001 0.0086 0.6127 0.0019 0.3200 0.0426 0.0001 0.0223 

 2532 309 1.2775 0.0041 0.7150 0.0149 0.0000 0.0083 0.5357 0.0017 0.2998 0.0529 0.0002 0.0296 

 2533 366 1.6978 0.0046 0.8271 0.0058 0.0000 0.0028 0.7501 0.0021 0.3654 0.0904 0.0002 0.0440 

 2534 326 1.4532 0.0045 0.7740 0.0148 0.0000 0.0079 0.6604 0.0020 0.3518 0.0379 0.0001 0.0202 

 2535 299 1.2094 0.0040 0.7108 0.0141 0.0000 0.0083 0.5664 0.0019 0.3329 0.0474 0.0002 0.0279 

 2537 348 1.7359 0.0050 0.8677 0.0165 0.0000 0.0082 0.6482 0.0019 0.3240 0.0629 0.0002 0.0314 

 2538 343 1.3408 0.0039 0.6524 0.0213 0.0001 0.0104 0.5947 0.0017 0.2894 0.0516 0.0002 0.0251 

 2539 315 1.4580 0.0046 0.7140 0.0167 0.0001 0.0082 0.5585 0.0018 0.2735 0.0477 0.0002 0.0234 

 2540 278 1.4775 0.0053 0.7797 0.0099 0.0000 0.0052 0.5794 0.0021 0.3058 0.0424 0.0002 0.0224 

 2541 310 1.5219 0.0049 0.7906 0.0132 0.0000 0.0069 0.5416 0.0017 0.2814 0.0559 0.0002 0.0290 

 2542 298 1.3924 0.0047 0.7310 0.0175 0.0001 0.0092 0.5906 0.0020 0.3100 0.0706 0.0002 0.0371 

 2543 PNMD 322 1.4711 0.0046 0.7777 0.0144 0.0000 0.0076 0.5190 0.0016 0.2744 0.0462 0.0001 0.0244 

 2545 331 1.4575 0.0044 0.7524 0.0157 0.0000 0.0081 0.6932 0.0021 0.3579 0.0649 0.0002 0.0335 

 2546 314 1.5393 0.0049 0.8073 0.0148 0.0000 0.0078 0.6631 0.0021 0.3478 0.0571 0.0002 0.0299 

 2547 265 1.4163 0.0053 0.7010 0.0168 0.0001 0.0083 0.5543 0.0021 0.2744 0.0440 0.0002 0.0218 

 2548 308 1.3839 0.0045 0.6926 0.0137 0.0000 0.0069 0.4858 0.0016 0.2431 0.0664 0.0002 0.0332 

 2550 293 1.3361 0.0046 0.7538 0.0180 0.0001 0.0102 0.6464 0.0022 0.3647 0.0382 0.0001 0.0216 

 2551 327 1.6982 0.0052 0.8629 0.0183 0.0001 0.0093 0.6772 0.0021 0.3441 0.0541 0.0002 0.0275 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE 
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 485 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN OVARY RIGHT 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 303 1.5313 0.0051 0.7847 0.0182 0.0001 0.0093 0.5141 0.0017 0.2635 0.0548 0.0002 0.0281 

 3528 355 1.6385 0.0046 0.8163 0.0150 0.0000 0.0075 0.6621 0.0019 0.3299 0.0750 0.0002 0.0374 

 3529 287 1.3586 0.0047 0.7257 0.0113 0.0000 0.0060 0.5545 0.0019 0.2962 0.0672 0.0002 0.0359 

 3531 310 1.5106 0.0049 0.8017 0.0131 0.0000 0.0070 0.6387 0.0021 0.3390 0.0587 0.0002 0.0312 

 3532 297 1.4079 0.0047 0.7394 0.0127 0.0000 0.0067 0.6935 0.0023 0.3642 0.0629 0.0002 0.0330 

 3533 305 1.3556 0.0044 0.6875 0.0134 0.0000 0.0068 0.5213 0.0017 0.2644 0.0665 0.0002 0.0337 

 3535 275 1.3202 0.0048 0.7226 0.0165 0.0001 0.0090 0.4794 0.0017 0.2624 0.0621 0.0002 0.0340 

 3536 304 1.4837 0.0049 0.7325 0.0152 0.0000 0.0075 0.6035 0.0020 0.2980 0.0566 0.0002 0.0279 

 3537 288 1.4193 0.0049 0.7099 0.0170 0.0001 0.0085 0.5084 0.0018 0.2543 0.0547 0.0002 0.0274 

 3538 326 1.3836 0.0042 0.7176 0.0148 0.0000 0.0077 0.5582 0.0017 0.2895 0.0553 0.0002 0.0287 

 3539 343 1.4865 0.0043 0.7508 0.0170 0.0000 0.0086 0.6979 0.0020 0.3525 0.0622 0.0002 0.0314 

 3540 320 1.5132 0.0047 0.7725 0.0131 0.0000 0.0067 0.6758 0.0021 0.3450 0.0588 0.0002 0.0300 

 3541 339 1.6717 0.0049 0.8610 0.0161 0.0000 0.0083 0.7774 0.0023 0.4004 0.0446 0.0001 0.0230 

 3542 316 1.4166 0.0045 0.7315 0.0140 0.0000 0.0072 0.6194 0.0020 0.3198 0.0547 0.0002 0.0282 

 3543 291 1.4616 0.0050 0.7845 0.0132 0.0000 0.0071 0.6823 0.0023 0.3662 0.0445 0.0002 0.0239 

 3544 287 1.2889 0.0045 0.7347 0.0108 0.0000 0.0062 0.5717 0.0020 0.3259 0.0555 0.0002 0.0316 

 3545 336 1.5393 0.0046 0.8009 0.0156 0.0000 0.0081 0.6036 0.0018 0.3141 0.0536 0.0002 0.0279 

 3546 335 1.6583 0.0050 0.8589 0.0132 0.0000 0.0068 0.6720 0.0020 0.3481 0.0394 0.0001 0.0204 

 3547 275 1.3278 0.0048 0.7010 0.0134 0.0000 0.0071 0.5253 0.0019 0.2773 0.0542 0.0002 0.0286 

 3548 319 1.6905 0.0053 0.8770 0.0136 0.0000 0.0071 0.5809 0.0018 0.3014 0.0449 0.0001 0.0233 

 3549 289 1.4431 0.0050 0.7291 0.0147 0.0001 0.0074 0.6544 0.0023 0.3306 0.0685 0.0002 0.0346 

 3550 309 1.6271 0.0053 0.8299 0.0131 0.0000 0.0067 0.5301 0.0017 0.2704 0.0445 0.0001 0.0227 

 3551 318 1.4594 0.0046 0.7699 0.0189 0.0001 0.0100 0.5338 0.0017 0.2816 0.0592 0.0002 0.0312 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 486 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 LUNGS PITUITARY SPLEEN OVARY RIGHT 

 

Animal FINAL BODY ------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 288 1.4435 0.0050 0.7266 0.0145 0.0001 0.0073 0.5439 0.0019 0.2738 0.0481 0.0002 0.0242 

 4527 306 1.2743 0.0042 0.6317 0.0119 0.0000 0.0059 0.4832 0.0016 0.2396 0.0490 0.0002 0.0243 

 4528 308 1.6807 0.0055 0.8276 0.0184 0.0001 0.0091 0.7423 0.0024 0.3655 0.0686 0.0002 0.0338 

 4529 353 1.8822 0.0053 0.9395 0.0198 0.0001 0.0099 0.6343 0.0018 0.3166 0.0843 0.0002 0.0421 

 4530 PNMD 307 1.4619 0.0048 0.8047 0.0106 0.0000 0.0058 0.6494 0.0021 0.3574 0.0832 0.0003 0.0458 

 4531 317 1.4770 0.0047 0.7510 0.0149 0.0000 0.0076 0.9196 0.0029 0.4676 0.0752 0.0002 0.0382 

 4532 300 1.4534 0.0048 0.7751 0.0140 0.0000 0.0075 0.5873 0.0020 0.3132 0.0486 0.0002 0.0259 

 4533 303 1.4590 0.0048 0.7611 0.0114 0.0000 0.0059 0.5999 0.0020 0.3129 0.0443 0.0001 0.0231 

 4534 300 1.6009 0.0053 0.8621 0.0149 0.0000 0.0080 0.6647 0.0022 0.3580 0.0580 0.0002 0.0312 

 4535 352 1.6058 0.0046 0.7878 0.0221 0.0001 0.0108 0.6429 0.0018 0.3154 0.0466 0.0001 0.0229 

 4536 345 1.5805 0.0046 0.8081 0.0157 0.0000 0.0080 0.5700 0.0017 0.2914 0.0627 0.0002 0.0321 

 4537 312 1.3715 0.0044 0.7246 0.0201 0.0001 0.0106 0.5868 0.0019 0.3100 0.0520 0.0002 0.0275 

 4539 300 1.3932 0.0046 0.7579 0.0137 0.0000 0.0075 0.5452 0.0018 0.2966 0.0389 0.0001 0.0212 

 4540 359 1.7763 0.0049 0.8611 0.0199 0.0001 0.0096 0.7263 0.0020 0.3521 0.0612 0.0002 0.0297 

 4541 290 1.3960 0.0048 0.7223 0.0145 0.0001 0.0075 0.5728 0.0020 0.2964 0.0725 0.0003 0.0375 

 4542 268 1.3239 0.0049 0.7069 0.0102 0.0000 0.0054 0.5176 0.0019 0.2764 0.0416 0.0002 0.0222 

 4545 293 1.4014 0.0048 0.7597 0.0119 0.0000 0.0065 0.6004 0.0021 0.3255 0.0544 0.0002 0.0295 

 4546 352 1.6520 0.0047 0.8590 0.0184 0.0001 0.0096 0.6920 0.0020 0.3598 0.0596 0.0002 0.0310 

 4547 365 1.5637 0.0043 0.7626 0.0192 0.0001 0.0094 0.6160 0.0017 0.3004 0.0651 0.0002 0.0317 

 4548 271 1.1615 0.0043 0.6097 0.0135 0.0000 0.0071 0.5294 0.0020 0.2779 0.0624 0.0002 0.0328 

 4549 280 1.3490 0.0048 0.6824 0.0187 0.0001 0.0095 0.5736 0.0020 0.2902 0.0507 0.0002 0.0256 

 4550 282 1.4316 0.0051 0.7515 0.0150 0.0001 0.0079 0.5284 0.0019 0.2774 0.0590 0.0002 0.0310 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE 
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 487 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 OVARY LEFT OVARIES UTERUS/OVIDUCTS 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 268 0.0473 0.0002 0.0245 0.0988 0.0004 0.0511 0.5877 0.0022 0.3038 

 1527 306 0.0390 0.0001 0.0210 0.0734 0.0002 0.0395 0.9044 0.0030 0.4866 

 1528 269 0.0575 0.0002 0.0292 0.1293 0.0005 0.0656 0.6518 0.0024 0.3309 

 1529 309 0.0626 0.0002 0.0312 0.1291 0.0004 0.0642 0.7285 0.0024 0.3625 

 1530 327 0.0509 0.0002 0.0265 0.1028 0.0003 0.0535 0.5036 0.0015 0.2619 

 1531 295 0.0378 0.0001 0.0201 0.0985 0.0003 0.0524 0.4778 0.0016 0.2540 

 1532 316 0.0430 0.0001 0.0228 0.0930 0.0003 0.0493 0.7759 0.0025 0.4116 

 1533 329 0.0584 0.0002 0.0288 0.1165 0.0004 0.0575 0.5997 0.0018 0.2958 

 1535 311 0.0450 0.0001 0.0233 0.0960 0.0003 0.0496 1.0705 0.0034 0.5534 

 1536 331 0.0337 0.0001 0.0167 0.0859 0.0003 0.0426 0.5840 0.0018 0.2898 

 1537 342 0.0727 0.0002 0.0359 0.1166 0.0003 0.0575 0.4749 0.0014 0.2342 

 1539 339 0.0708 0.0002 0.0356 0.1513 0.0004 0.0761 0.5855 0.0017 0.2945 

 1541 335 0.0476 0.0001 0.0234 0.1141 0.0003 0.0560 1.0390 0.0031 0.5102 

 1542 300 0.0848 0.0003 0.0457 0.1504 0.0005 0.0811 0.8168 0.0027 0.4405 

 1543 311 0.0627 0.0002 0.0355 0.1443 0.0005 0.0816 0.9533 0.0031 0.5393 

 1544 344 0.0439 0.0001 0.0228 0.1020 0.0003 0.0529 0.5109 0.0015 0.2652 

 1545 284 0.0662 0.0002 0.0338 0.1165 0.0004 0.0595 0.6452 0.0023 0.3295 

 1546 304 0.0464 0.0002 0.0233 0.1045 0.0003 0.0524 0.7678 0.0025 0.3853 

 1547 291 0.0393 0.0001 0.0203 0.0876 0.0003 0.0452 0.5400 0.0019 0.2789 

 1550 268 0.0450 0.0002 0.0223 0.0969 0.0004 0.0480 0.5383 0.0020 0.2665 

 1551 292 0.0570 0.0002 0.0318 0.1030 0.0004 0.0576 0.9440 0.0032 0.5275 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 488 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 OVARY LEFT OVARIES UTERUS/OVIDUCTS 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 303 0.0844 0.0003 0.0447 0.1489 0.0005 0.0789 1.2438 0.0041 0.6590 

 2527 291 0.0559 0.0002 0.0295 0.1258 0.0004 0.0665 0.7070 0.0024 0.3735 

 2528 330 0.0492 0.0001 0.0249 0.0985 0.0003 0.0499 0.6708 0.0020 0.3396 

 2529 292 0.0402 0.0001 0.0213 0.0805 0.0003 0.0426 0.4501 0.0015 0.2381 

 2530 291 0.0504 0.0002 0.0264 0.1128 0.0004 0.0591 0.7609 0.0026 0.3989 

 2531 321 0.0538 0.0002 0.0281 0.0974 0.0003 0.0509 0.5662 0.0018 0.2957 

 2532 309 0.0399 0.0001 0.0223 0.0940 0.0003 0.0526 0.7779 0.0025 0.4354 

 2533 366 0.0686 0.0002 0.0334 0.1618 0.0004 0.0788 0.5392 0.0015 0.2627 

 2534 326 0.0618 0.0002 0.0329 0.0999 0.0003 0.0532 0.9750 0.0030 0.5193 

 2535 299 0.0415 0.0001 0.0244 0.0896 0.0003 0.0527 0.6396 0.0021 0.3759 

 2537 348 0.0452 0.0001 0.0226 0.1070 0.0003 0.0535 0.5791 0.0017 0.2895 

 2538 343 0.0612 0.0002 0.0298 0.1113 0.0003 0.0542 0.5657 0.0016 0.2753 

 2539 315 0.0494 0.0002 0.0242 0.0971 0.0003 0.0476 0.5778 0.0018 0.2830 

 2540 278 0.0617 0.0002 0.0326 0.1024 0.0004 0.0540 0.5409 0.0019 0.2854 

 2541 310 0.0720 0.0002 0.0374 0.1285 0.0004 0.0668 0.5194 0.0017 0.2698 

 2542 298 0.0579 0.0002 0.0304 0.1262 0.0004 0.0663 0.8274 0.0028 0.4344 

 2543 PNMD 322 0.0566 0.0002 0.0299 0.1032 0.0003 0.0546 0.7186 0.0022 0.3799 

 2545 331 0.0458 0.0001 0.0236 0.1121 0.0003 0.0579 0.9739 0.0029 0.5028 

 2546 314 0.0551 0.0002 0.0289 0.1112 0.0004 0.0583 0.5610 0.0018 0.2942 

 2547 265 0.0483 0.0002 0.0239 0.0911 0.0003 0.0451 0.4982 0.0019 0.2466 

 2548 308 0.0535 0.0002 0.0268 0.1191 0.0004 0.0596 0.7594 0.0025 0.3800 

 2550 293 0.0656 0.0002 0.0370 0.1022 0.0003 0.0577 0.8173 0.0028 0.4611 

 2551 327 0.0570 0.0002 0.0290 0.1105 0.0003 0.0561 0.9137 0.0028 0.4643 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE 
11-JAN-2010 18:09

Huntingdon Life Sciences 00-4205 PAGE 489 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 OVARY LEFT OVARIES UTERUS/OVIDUCTS 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 303 0.0539 0.0002 0.0276 0.1082 0.0004 0.0554 0.6043 0.0020 0.3097 

 3528 355 0.0658 0.0002 0.0328 0.1390 0.0004 0.0693 0.6980 0.0020 0.3477 

 3529 287 0.0547 0.0002 0.0292 0.1198 0.0004 0.0640 0.6603 0.0023 0.3527 

 3531 310 0.0665 0.0002 0.0353 0.1208 0.0004 0.0641 0.7209 0.0023 0.3826 

 3532 297 0.0518 0.0002 0.0272 0.1124 0.0004 0.0590 0.4865 0.0016 0.2555 

 3533 305 0.0705 0.0002 0.0358 0.1377 0.0005 0.0698 0.5298 0.0017 0.2687 

 3535 275 0.0532 0.0002 0.0291 0.1145 0.0004 0.0627 0.5928 0.0022 0.3245 

 3536 304 0.0581 0.0002 0.0287 0.1143 0.0004 0.0564 0.5523 0.0018 0.2727 

 3537 288 0.0418 0.0001 0.0209 0.0969 0.0003 0.0485 1.1840 0.0041 0.5922 

 3538 326 0.0465 0.0001 0.0241 0.1021 0.0003 0.0530 0.5887 0.0018 0.3053 

 3539 343 0.0466 0.0001 0.0235 0.1145 0.0003 0.0578 0.4864 0.0014 0.2457 

 3540 320 0.0495 0.0002 0.0253 0.1083 0.0003 0.0553 0.6317 0.0020 0.3225 

 3541 339 0.0417 0.0001 0.0215 0.0822 0.0002 0.0423 0.4900 0.0014 0.2524 

 3542 316 0.0610 0.0002 0.0315 0.1156 0.0004 0.0597 0.5714 0.0018 0.2950 

 3543 291 0.0308 0.0001 0.0165 0.0755 0.0003 0.0405 0.4602 0.0016 0.2470 

 3544 287 0.0415 0.0001 0.0237 0.0968 0.0003 0.0552 0.5992 0.0021 0.3416 

 3545 336 0.0400 0.0001 0.0208 0.0945 0.0003 0.0492 0.5435 0.0016 0.2828 

 3546 335 0.0600 0.0002 0.0311 0.0960 0.0003 0.0497 0.5125 0.0015 0.2654 

 3547 275 0.0560 0.0002 0.0296 0.1083 0.0004 0.0572 0.4475 0.0016 0.2362 

 3548 319 0.0557 0.0002 0.0289 0.1174 0.0004 0.0609 0.6893 0.0022 0.3576 

 3549 289 0.0621 0.0002 0.0314 0.1291 0.0004 0.0652 0.6766 0.0023 0.3418 

 3550 309 0.0578 0.0002 0.0295 0.1014 0.0003 0.0517 0.5997 0.0019 0.3059 

 3551 318 0.0580 0.0002 0.0306 0.1168 0.0004 0.0616 0.5997 0.0019 0.3164 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT
11-JAN-2010 18:09 

Huntingdon Life Sciences 00-4205 PAGE 490 

 

 APPENDIX X P0 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 ORGAN AND FINAL BODY WEIGHTS 

FEMALES GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 OVARY LEFT OVARIES UTERUS/OVIDUCTS 

 

Animal FINAL BODY ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

 g g g g 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 288 0.0524 0.0002 0.0264 0.0995 0.0003 0.0501 0.5068 0.0018 0.2551 

 4527 306 0.0406 0.0001 0.0201 0.0898 0.0003 0.0445 0.7180 0.0023 0.3560 

 4528 308 0.0675 0.0002 0.0332 0.1355 0.0004 0.0667 0.6311 0.0021 0.3108 

 4529 353 0.0580 0.0002 0.0290 0.1414 0.0004 0.0706 1.0146 0.0029 0.5064 

 4530 PNMD 307 0.0583 0.0002 0.0321 0.1423 0.0005 0.0783 0.4704 0.0015 0.2589 

 4531 317 0.0524 0.0002 0.0266 0.1259 0.0004 0.0640 0.6767 0.0021 0.3441 

 4532 300 0.0475 0.0002 0.0253 0.0961 0.0003 0.0513 0.4131 0.0014 0.2203 

 4533 303 0.0451 0.0001 0.0235 0.0909 0.0003 0.0474 0.4052 0.0013 0.2114 

 4534 300 0.0609 0.0002 0.0328 0.1178 0.0004 0.0634 1.0442 0.0035 0.5623 

 4535 352 0.0661 0.0002 0.0324 0.1080 0.0003 0.0530 0.6108 0.0017 0.2996 

 4536 345 0.0577 0.0002 0.0295 0.1199 0.0003 0.0613 0.5907 0.0017 0.3020 

 4537 312 0.0451 0.0001 0.0238 0.0979 0.0003 0.0517 0.9512 0.0030 0.5026 

 4539 300 0.0489 0.0002 0.0266 0.0848 0.0003 0.0461 0.6156 0.0020 0.3349 

 4540 359 0.0755 0.0002 0.0366 0.1344 0.0004 0.0652 0.5192 0.0014 0.2517 

 4541 290 0.0754 0.0003 0.0390 0.1476 0.0005 0.0764 0.6463 0.0022 0.3344 

 4542 268 0.0554 0.0002 0.0296 0.0969 0.0004 0.0517 0.4756 0.0018 0.2540 

 4545 293 0.0725 0.0002 0.0393 0.1274 0.0004 0.0691 0.7212 0.0025 0.3910 

 4546 352 0.0586 0.0002 0.0305 0.1145 0.0003 0.0595 0.9667 0.0027 0.5027 

 4547 365 0.0686 0.0002 0.0335 0.1333 0.0004 0.0650 0.9168 0.0025 0.4471 

 4548 271 0.0679 0.0003 0.0356 0.1302 0.0005 0.0683 0.7685 0.0028 0.4034 

 4549 280 0.0535 0.0002 0.0271 0.1073 0.0004 0.0543 0.6419 0.0023 0.3247 

 4550 282 0.0547 0.0002 0.0287 0.1137 0.0004 0.0597 0.4997 0.0018 0.2623 

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE 
Huntingdon Life Sciences 00-4205 Page 491

211-DIPE-1G Final Report

Individual Pup Organ Weights

Preface Appendix Y

Table of Contents

F1 Generation

Absolute Organ Weights and % Organ to Body Weight Ratios ........................ 492

Absolute Organ Weights and % Organ to Brain Weight Ratios ........................ 500

Key to Abbreviations:

g = Grams
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 492 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 4 M 87.3 1.6635 1.9055 0.2952 0.3381 0.4230 0.4845 

 10 F 75.1 1.5478 2.0610 0.2587 0.3445 0.3330 0.4434 

 1527 8 M 86.4 1.7058 1.9743 0.3929 0.4547 0.3893 0.4506 

 11 F 80.1 1.5981 1.9951 0.3573 0.4461 0.4231 0.5282 

 1528 5 M 91.6 1.6295 1.7789 0.4421 0.4826 0.4600 0.5022 

 9 F 87.4 1.5541 1.7781 0.3883 0.4443 0.4554 0.5211 

 1529 2 M 87.0 1.5788 1.8147 0.3681 0.4231 0.2972 0.3416 

 10 F 79.1 1.4446 1.8263 0.3129 0.3956 0.3297 0.4168 

 1530 2 M 105.0 1.7142 1.6326 0.5212 0.4964 0.3831 0.3649 

 5 F 89.9 1.5861 1.7643 0.3204 0.3564 0.3807 0.4235 

 1531 1 M 64.0 1.5543 2.4286 0.2186 0.3416 0.2291 0.3580 

 10 F 59.1 1.5119 2.5582 0.2409 0.4076 0.2636 0.4460 

 1532 9 M 80.7 1.5049 1.8648 0.3813 0.4725 0.2565 0.3178 

 11 F 71.4 1.4989 2.0993 0.3181 0.4455 0.2338 0.3275 

 1533 3 M 83.8 1.5961 1.9047 0.3228 0.3852 0.4841 0.5777 

 13 F 79.5 1.6198 2.0375 0.3349 0.4213 0.3759 0.4728 

 1535 1 M 59.7 1.5108 2.5307 0.2321 0.3888 0.2323 0.3891 

 9 F 66.2 1.4593 2.2044 0.3095 0.4675 0.4214 0.6366 

 1536 4 M 83.3 1.6626 1.9959 0.3779 0.4537 0.3783 0.4541 

 8 F 82.7 1.5887 1.9210 0.3136 0.3792 0.3615 0.4371 

 1537 6 M 82.2 1.6615 2.0213 0.3632 0.4418 0.2512 0.3056 

 13 F 84.9 1.5728 1.8525 0.3423 0.4032 0.3648 0.4297 

 1539 7 M 88.1 1.6265 1.8462 0.4056 0.4604 0.4740 0.5380 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 493 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 1539 (continued)

 10 F 69.9 1.4850 2.1245 0.3068 0.4389 0.3819 0.5464 

 1541 7 M 87.6 1.6447 1.8775 0.3679 0.4200 0.3294 0.3760 

 12 F 79.4 1.5057 1.8963 0.2854 0.3594 0.3091 0.3893 

 1542 5 M 76.4 1.5558 2.0364 0.3315 0.4339 0.4013 0.5253 

 8 F 69.0 1.4586 2.1139 0.2665 0.3862 0.3418 0.4954 

 1543 6 M 78.6 1.5333 1.9508 0.3536 0.4499 0.2764 0.3517 

 8 F 77.1 1.5543 2.0160 0.4021 0.5215 0.3261 0.4230 

 1544 8 M 82.1 1.6006 1.9496 0.3313 0.4035 0.3084 0.3756 

 13 F 79.6 1.5836 1.9894 0.3876 0.4869 0.3634 0.4565 

 1545 2 M 66.7 1.4035 2.1042 0.2651 0.3975 0.2398 0.3595 

 11 F 70.9 1.4983 2.1133 0.3229 0.4554 0.2964 0.4181 

 1546 5 M 86.2 1.5629 1.8131 0.2981 0.3458 0.2779 0.3224 

 6 F 75.9 1.5404 2.0295 0.2415 0.3182 0.3008 0.3963 

 1547 4 M 79.1 1.5266 1.9300 0.3372 0.4263 0.3611 0.4565 

 6 F 79.5 1.5151 1.9058 0.3277 0.4122 0.3726 0.4687 

 1550 4 M 72.0 1.4867 2.0649 0.3018 0.4192 0.3157 0.4385 

 12 F 62.2 1.4719 2.3664 0.2059 0.3310 0.1574 0.2531 

 1551 2 M 80.7 1.5679 1.9429 0.3678 0.4558 0.3263 0.4043 

 6 F 65.6 1.4553 2.2184 0.2030 0.3095 0.3242 0.4942 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 494 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 11 M 78.3 1.6181 2.0665 0.2698 0.3446 0.4092 0.5226 

 16 F 69.3 1.5905 2.2951 0.2807 0.4051 0.3168 0.4571 

 2527 1 M 76.3 1.5988 2.0954 0.2659 0.3485 0.3047 0.3993 

 11 F 64.4 1.4222 2.2084 0.2500 0.3882 0.2632 0.4087 

 2528 3 M 74.2 1.5527 2.0926 0.3482 0.4693 0.3266 0.4402 

 14 F 66.2 1.4322 2.1634 0.2902 0.4384 0.3215 0.4856 

 2529 2 M 69.1 1.5433 2.2334 0.2654 0.3841 0.3642 0.5271 

 12 F 69.5 1.5262 2.1960 0.2988 0.4299 0.3287 0.4729 

 2530 6 M 95.6 1.6540 1.7301 0.3057 0.3198 0.3476 0.3636 

 12 F 82.6 1.5712 1.9022 0.3206 0.3881 0.4472 0.5414 

 2531 3 M 78.0 1.5916 2.0405 0.2910 0.3731 0.3806 0.4879 

 15 F 77.9 1.4933 1.9169 0.2941 0.3775 0.4895 0.6284 

 2532 9 M 79.7 1.5641 1.9625 0.3004 0.3769 0.3436 0.4311 

 13 F 75.2 1.5630 2.0785 0.2834 0.3769 0.3682 0.4896 

 2533 1 M 91.2 1.6443 1.8030 0.4076 0.4469 0.5279 0.5788 

 15 F 82.8 1.6163 1.9521 0.3943 0.4762 0.4913 0.5934 

 2534 4 M 75.8 1.6325 2.1537 0.3038 0.4008 0.4184 0.5520 

 11 F 72.2 1.4984 2.0753 0.2873 0.3979 0.4009 0.5553 

 2535 2 M 69.8 1.3928 1.9954 0.2751 0.3941 0.3546 0.5080 

 9 F 80.6 1.5202 1.8861 0.2767 0.3433 0.4022 0.4990 

 2537 3 M 105.5 1.6101 1.5262 0.3987 0.3779 0.5236 0.4963 

 4 F 88.9 1.5389 1.7310 0.3339 0.3756 0.4197 0.4721 

 2538 5 M 89.7 1.6768 1.8693 0.4748 0.5293 0.4829 0.5384 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 495 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

----------------------------------------------------------------------------------------------------------------------------------- 

 2538 (continued)

 10 F 77.4 1.5574 2.0121 0.3022 0.3904 0.4356 0.5628 

 2539 4 M 81.1 1.6087 1.9836 0.3032 0.3739 0.3365 0.4149 

 9 F 80.0 1.5591 1.9489 0.2889 0.3611 0.3161 0.3951 

 2540 6 M 80.4 1.5624 1.9433 0.3142 0.3908 0.3355 0.4173 

 7 F 80.2 1.5003 1.8707 0.3046 0.3798 0.3355 0.4183 

 2541 1 M 79.5 1.5409 1.9382 0.2880 0.3623 0.3293 0.4142 

 6 F 69.0 1.5358 2.2258 0.2679 0.3883 0.3044 0.4412 

 2542 5 M 77.2 1.4912 1.9316 0.3327 0.4310 0.3687 0.4776 

 8 F 77.3 1.4064 1.8194 0.2887 0.3735 0.3306 0.4277 

 2543 6 M 82.1 1.5275 1.8605 0.3209 0.3909 0.3853 0.4693 

 12 F 83.1 1.6215 1.9513 0.2981 0.3587 0.5042 0.6067 

 2545 1 M 73.1 1.5836 2.1663 0.3058 0.4183 0.2838 0.3882 

 12 F 72.8 1.5095 2.0735 0.2641 0.3628 0.3369 0.4628 

 2546 1 M 96.5 1.6034 1.6616 0.4144 0.4294 0.4221 0.4374 

 10 F 90.7 1.5840 1.7464 0.3410 0.3760 0.4184 0.4613 

 2547 4 M 71.6 1.6636 2.3235 0.2774 0.3874 0.2726 0.3807 

 9 F 64.6 1.5846 2.4529 0.2331 0.3608 0.2784 0.4310 

 2548 1 M 84.4 1.5975 1.8928 0.3106 0.3680 0.3081 0.3650 

 13 F 74.3 1.5052 2.0258 0.2803 0.3773 0.2923 0.3934 

 2550 2 M 42.3 1.2478 2.9499 0.2208 0.5220 0.2241 0.5298 

 8 F 70.6 1.5451 2.1885 0.3113 0.4409 0.3797 0.5378 

 2551 2 M 90.2 1.5684 1.7388 0.4391 0.4868 0.4607 0.5108 

 10 F 84.5 1.5600 1.8462 0.3733 0.4418 0.4498 0.5323 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 496 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 3 M 74.4 1.4606 1.9632 0.2870 0.3858 0.2996 0.4027 

 13 F 69.4 1.5148 2.1827 0.2561 0.3690 0.3588 0.5170 

 3528 2 M 77.2 1.5610 2.0220 0.3620 0.4689 0.3477 0.4504 

 15 F 82.7 1.6389 1.9817 0.3325 0.4021 0.4825 0.5834 

 3529 5 M 80.8 1.5563 1.9261 0.3459 0.4281 0.4578 0.5666 

 14 F 72.2 1.5221 2.1082 0.2726 0.3776 0.3983 0.5517 

 3531 5 M 41.9 1.4318 3.4172 0.1480 0.3532 0.1180 0.2816 

 9 F 65.6 1.4859 2.2651 0.2530 0.3857 0.3048 0.4646 

 3532 9 F 83.8 1.6259 1.9402 0.3346 0.3993 0.4296 0.5126 

 12 M 81.3 1.6251 1.9989 0.3272 0.4025 0.4481 0.5512 

 3533 1 M 68.1 1.5061 2.2116 0.2652 0.3894 0.2893 0.4248 

 13 F 60.3 1.4384 2.3854 0.1954 0.3240 0.3448 0.5718 

 3535 1 M 60.4 1.4675 2.4296 0.2392 0.3960 0.2253 0.3730 

 15 F 55.9 1.4045 2.5125 0.1782 0.3188 0.2591 0.4635 

 3536 2 M 77.3 1.6217 2.0979 0.3359 0.4345 0.4429 0.5730 

 10 F 70.5 1.5073 2.1380 0.2840 0.4028 0.3768 0.5345 

 3537 3 M 86.3 1.6212 1.8786 0.3290 0.3812 0.4376 0.5071 

 13 F 75.8 1.5736 2.0760 0.2926 0.3860 0.3384 0.4464 

 3538 1 M 77.5 1.6451 2.1227 0.2985 0.3852 0.3570 0.4606 

 11 F 77.5 1.5596 2.0124 0.3369 0.4347 0.3746 0.4834 

 3539 5 M 85.2 1.6564 1.9441 0.4276 0.5019 0.3941 0.4626 

 11 F 67.4 1.5593 2.3135 0.2644 0.3923 0.2913 0.4322 

 3540 2 M 85.6 1.5612 1.8238 0.3886 0.4540 0.3800 0.4439 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 497 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 3540 (continued)

 13 F 80.2 1.5592 1.9441 0.3123 0.3894 0.4793 0.5976 

 3541 5 M 98.4 1.6801 1.7074 0.4746 0.4823 0.4214 0.4283 

 14 F 89.8 1.5283 1.7019 0.3730 0.4154 0.4039 0.4498 

 3542 1 M 87.2 1.7123 1.9636 0.4146 0.4755 0.4047 0.4641 

 8 F 80.4 1.6336 2.0318 0.2808 0.3493 0.4060 0.5050 

 3543 3 M 71.8 1.4558 2.0276 0.2913 0.4057 0.3286 0.4577 

 9 F 71.7 1.4384 2.0061 0.2647 0.3692 0.3486 0.4862 

 3544 2 M 70.2 1.4253 2.0303 0.4062 0.5786 0.3154 0.4493 

 10 F 68.5 1.3822 2.0178 0.3317 0.4842 0.3384 0.4940 

 3545 3 M 91.3 1.6104 1.7639 0.3438 0.3766 0.3912 0.4285 

 13 F 78.4 1.5311 1.9529 0.2695 0.3437 0.3564 0.4546 

 3546 6 M 89.9 1.6226 1.8049 0.4419 0.4915 0.3793 0.4219 

 10 F 73.9 1.5126 2.0468 0.3273 0.4429 0.3550 0.4804 

 3547 6 M 78.4 1.4517 1.8517 0.3239 0.4131 0.3827 0.4881 

 7 F 67.5 1.3869 2.0547 0.3343 0.4953 0.3582 0.5307 

 3548 2 M 92.3 1.5685 1.6993 0.3662 0.3967 0.5282 0.5723 

 9 F 77.2 1.5315 1.9838 0.2989 0.3872 0.4246 0.5500 

 3549 2 M 80.8 1.6042 1.9854 0.4282 0.5300 0.4164 0.5153 

 8 F 73.9 1.5253 2.0640 0.3449 0.4667 0.4253 0.5755 

 3550 7 M 63.8 1.5579 2.4418 0.2227 0.3491 0.2234 0.3502 

 13 F 69.6 1.5527 2.2309 0.3180 0.4569 0.3543 0.5091 

 3551 5 M 88.6 1.6458 1.8576 0.3949 0.4457 0.4535 0.5119 

 14 F 76.1 1.4841 1.9502 0.2593 0.3407 0.3728 0.4899 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 498 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 6 M 69.4 1.5900 2.2911 0.2610 0.3761 0.3437 0.4952 

 13 F 65.9 1.5093 2.2903 0.2536 0.3848 0.2959 0.4490 

 4527 2 M 82.3 1.6845 2.0468 0.2989 0.3632 0.3947 0.4796 

 10 F 72.9 1.5719 2.1562 0.2577 0.3535 0.3715 0.5096 

 4528 5 M 73.3 1.5686 2.1400 0.3223 0.4397 0.3162 0.4314 

 11 F 68.6 1.4889 2.1704 0.2670 0.3892 0.3539 0.5159 

 4529 3 M 77.1 1.6241 2.1065 0.3153 0.4089 0.2591 0.3361 

 15 F 77.3 1.5165 1.9618 0.2907 0.3761 0.3411 0.4413 

 4530 3 M 73.3 1.5800 2.1555 0.2314 0.3157 0.2733 0.3729 

 7 F 73.0 1.4575 1.9966 0.2425 0.3322 0.3562 0.4879 

 4531 3 M 71.9 1.5429 2.1459 0.3105 0.4318 0.3014 0.4192 

 9 F 71.3 1.4720 2.0645 0.3094 0.4339 0.3167 0.4442 

 4532 8 M 92.5 1.6124 1.7431 0.4105 0.4438 0.4977 0.5381 

 11 F 74.1 1.5440 2.0837 0.3080 0.4157 0.3749 0.5059 

 4533 6 M 64.3 1.4033 2.1824 0.3446 0.5359 0.3006 0.4675 

 9 F 69.2 1.4734 2.1292 0.3914 0.5656 0.2759 0.3987 

 4534 4 M 85.4 1.6063 1.8809 0.3910 0.4578 0.3234 0.3787 

 12 F 80.4 1.5083 1.8760 0.2976 0.3701 0.3687 0.4586 

 4535 6 M 74.0 1.5830 2.1392 0.2355 0.3182 0.3228 0.4362 

 15 F 74.5 1.5242 2.0459 0.2357 0.3164 0.3881 0.5209 

 4536 6 M 89.5 1.6077 1.7963 0.3940 0.4402 0.4485 0.5011 

 9 F 73.5 1.5048 2.0473 0.2562 0.3486 0.3505 0.4769 

 4537 2 M 70.6 1.5821 2.2409 0.2514 0.3561 0.2224 0.3150 
11-JAN-2010 18:16

Huntingdon Life Sciences 00-4205 PAGE 499 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%) 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 4537 (continued)

 9 F 83.9 1.5349 1.8294 0.3574 0.4260 0.3401 0.4054 

 4539 3 M 84.0 1.6141 1.9215 0.2813 0.3349 0.2915 0.3470 

 8 F 84.2 1.5236 1.8095 0.3069 0.3645 0.4142 0.4919 

 4540 6 M 88.1 1.5921 1.8072 0.3782 0.4293 0.4326 0.4910 

 12 F 77.2 1.5358 1.9894 0.3555 0.4605 0.4391 0.5688 

 4541 3 M 70.7 1.5188 2.1482 0.3050 0.4314 0.2143 0.3031 

 12 F 79.4 1.4771 1.8603 0.3549 0.4470 0.3694 0.4652 

 4542 2 M 92.7 1.5190 1.6386 0.3666 0.3955 0.5598 0.6039 

 7 F 80.0 1.5489 1.9361 0.2422 0.3027 0.4369 0.5461 

 4545 6 M 75.5 1.5021 1.9895 0.3198 0.4236 0.4134 0.5475 

 11 F 63.6 1.4356 2.2572 0.2223 0.3495 0.3621 0.5693 

 4546 4 M 72.3 1.5617 2.1600 0.3026 0.4185 0.3637 0.5030 

 10 F 70.2 1.4631 2.0842 0.2413 0.3437 0.3731 0.5315 

 4547 2 M 88.1 1.5124 1.7167 0.3743 0.4249 0.3504 0.3977 

 13 F 76.7 1.5273 1.9913 0.2870 0.3742 0.3658 0.4769 

 4548 1 M 58.6 1.4496 2.4737 0.2164 0.3693 0.2596 0.4430 

 12 F 65.7 1.5342 2.3352 0.2643 0.4023 0.3383 0.5149 

 4549 6 M 78.4 1.5552 1.9837 0.2918 0.3722 0.3282 0.4186 

 10 F 73.6 1.5510 2.1073 0.2864 0.3891 0.2909 0.3952 

 4550 8 M 69.9 1.5266 2.1840 0.2512 0.3594 0.3516 0.5030 

 12 F 75.1 1.5166 2.0194 0.2872 0.3824 0.3565 0.4747 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 500 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 1526 4 M 1.6635 0.2952 17.746 0.4230 25.428 

 10 F 1.5478 0.2587 16.714 0.3330 21.514 

 1527 8 M 1.7058 0.3929 23.033 0.3893 22.822 

 11 F 1.5981 0.3573 22.358 0.4231 26.475 

 1528 5 M 1.6295 0.4421 27.131 0.4600 28.230 

 9 F 1.5541 0.3883 24.986 0.4554 29.303 

 1529 2 M 1.5788 0.3681 23.315 0.2972 18.824 

 10 F 1.4446 0.3129 21.660 0.3297 22.823 

 1530 2 M 1.7142 0.5212 30.405 0.3831 22.349 

 5 F 1.5861 0.3204 20.200 0.3807 24.002 

 1531 1 M 1.5543 0.2186 14.064 0.2291 14.740 

 10 F 1.5119 0.2409 15.934 0.2636 17.435 

 1532 9 M 1.5049 0.3813 25.337 0.2565 17.044 

 11 F 1.4989 0.3181 21.222 0.2338 15.598 

 1533 3 M 1.5961 0.3228 20.224 0.4841 30.330 

 13 F 1.6198 0.3349 20.675 0.3759 23.207 

 1535 1 M 1.5108 0.2321 15.363 0.2323 15.376 

 9 F 1.4593 0.3095 21.209 0.4214 28.877 

 1536 4 M 1.6626 0.3779 22.729 0.3783 22.754 

 8 F 1.5887 0.3136 19.739 0.3615 22.754 

 1537 6 M 1.6615 0.3632 21.860 0.2512 15.119 

 13 F 1.5728 0.3423 21.764 0.3648 23.194 

 1539 7 M 1.6265 0.4056 24.937 0.4740 29.142 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 501 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP I 0 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 1539 (continued)

 10 F 1.4850 0.3068 20.660 0.3819 25.717 

 1541 7 M 1.6447 0.3679 22.369 0.3294 20.028 

 12 F 1.5057 0.2854 18.955 0.3091 20.529 

 1542 5 M 1.5558 0.3315 21.307 0.4013 25.794 

 8 F 1.4586 0.2665 18.271 0.3418 23.433 

 1543 6 M 1.5333 0.3536 23.061 0.2764 18.026 

 8 F 1.5543 0.4021 25.870 0.3261 20.981 

 1544 8 M 1.6006 0.3313 20.698 0.3084 19.268 

 13 F 1.5836 0.3876 24.476 0.3634 22.948 

 1545 2 M 1.4035 0.2651 18.888 0.2398 17.086 

 11 F 1.4983 0.3229 21.551 0.2964 19.782 

 1546 5 M 1.5629 0.2981 19.074 0.2779 17.781 

 6 F 1.5404 0.2415 15.678 0.3008 19.527 

 1547 4 M 1.5266 0.3372 22.088 0.3611 23.654 

 6 F 1.5151 0.3277 21.629 0.3726 24.592 

 1550 4 M 1.4867 0.3018 20.300 0.3157 21.235 

 12 F 1.4719 0.2059 13.989 0.1574 10.694 

 1551 2 M 1.5679 0.3678 23.458 0.3263 20.811 

 6 F 1.4553 0.2030 13.949 0.3242 22.277 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 502 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 2526 11 M 1.6181 0.2698 16.674 0.4092 25.289 

 16 F 1.5905 0.2807 17.649 0.3168 19.918 

 2527 1 M 1.5988 0.2659 16.631 0.3047 19.058 

 11 F 1.4222 0.2500 17.578 0.2632 18.507 

 2528 3 M 1.5527 0.3482 22.425 0.3266 21.034 

 14 F 1.4322 0.2902 20.263 0.3215 22.448 

 2529 2 M 1.5433 0.2654 17.197 0.3642 23.599 

 12 F 1.5262 0.2988 19.578 0.3287 21.537 

 2530 6 M 1.6540 0.3057 18.482 0.3476 21.016 

 12 F 1.5712 0.3206 20.405 0.4472 28.462 

 2531 3 M 1.5916 0.2910 18.283 0.3806 23.913 

 15 F 1.4933 0.2941 19.695 0.4895 32.780 

 2532 9 M 1.5641 0.3004 19.206 0.3436 21.968 

 13 F 1.5630 0.2834 18.132 0.3682 23.557 

 2533 1 M 1.6443 0.4076 24.789 0.5279 32.105 

 15 F 1.6163 0.3943 24.395 0.4913 30.397 

 2534 4 M 1.6325 0.3038 18.609 0.4184 25.629 

 11 F 1.4984 0.2873 19.174 0.4009 26.755 

 2535 2 M 1.3928 0.2751 19.752 0.3546 25.460 

 9 F 1.5202 0.2767 18.202 0.4022 26.457 

 2537 3 M 1.6101 0.3987 24.762 0.5236 32.520 

 4 F 1.5389 0.3339 21.697 0.4197 27.273 

 2538 5 M 1.6768 0.4748 28.316 0.4829 28.799 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 503 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP II 2000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 2538 (continued)

 10 F 1.5574 0.3022 19.404 0.4356 27.970 

 2539 4 M 1.6087 0.3032 18.848 0.3365 20.918 

 9 F 1.5591 0.2889 18.530 0.3161 20.275 

 2540 6 M 1.5624 0.3142 20.110 0.3355 21.473 

 7 F 1.5003 0.3046 20.303 0.3355 22.362 

 2541 1 M 1.5409 0.2880 18.690 0.3293 21.371 

 6 F 1.5358 0.2679 17.444 0.3044 19.820 

 2542 5 M 1.4912 0.3327 22.311 0.3687 24.725 

 8 F 1.4064 0.2887 20.528 0.3306 23.507 

 2543 6 M 1.5275 0.3209 21.008 0.3853 25.224 

 12 F 1.6215 0.2981 18.384 0.5042 31.095 

 2545 1 M 1.5836 0.3058 19.310 0.2838 17.921 

 12 F 1.5095 0.2641 17.496 0.3369 22.319 

 2546 1 M 1.6034 0.4144 25.845 0.4221 26.325 

 10 F 1.5840 0.3410 21.528 0.4184 26.414 

 2547 4 M 1.6636 0.2774 16.675 0.2726 16.386 

 9 F 1.5846 0.2331 14.710 0.2784 17.569 

 2548 1 M 1.5975 0.3106 19.443 0.3081 19.286 

 13 F 1.5052 0.2803 18.622 0.2923 19.419 

 2550 2 M 1.2478 0.2208 17.695 0.2241 17.960 

 8 F 1.5451 0.3113 20.148 0.3797 24.574 

 2551 2 M 1.5684 0.4391 27.997 0.4607 29.374 

 10 F 1.5600 0.3733 23.929 0.4498 28.833 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 504 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 3527 3 M 1.4606 0.2870 19.649 0.2996 20.512 

 13 F 1.5148 0.2561 16.907 0.3588 23.686 

 3528 2 M 1.5610 0.3620 23.190 0.3477 22.274 

 15 F 1.6389 0.3325 20.288 0.4825 29.440 

 3529 5 M 1.5563 0.3459 22.226 0.4578 29.416 

 14 F 1.5221 0.2726 17.909 0.3983 26.168 

 3531 5 M 1.4318 0.1480 10.337 0.1180 8.241 

 9 F 1.4859 0.2530 17.027 0.3048 20.513 

 3532 9 F 1.6259 0.3346 20.579 0.4296 26.422 

 12 M 1.6251 0.3272 20.134 0.4481 27.574 

 3533 1 M 1.5061 0.2652 17.608 0.2893 19.209 

 13 F 1.4384 0.1954 13.585 0.3448 23.971 

 3535 1 M 1.4675 0.2392 16.300 0.2253 15.353 

 15 F 1.4045 0.1782 12.688 0.2591 18.448 

 3536 2 M 1.6217 0.3359 20.713 0.4429 27.311 

 10 F 1.5073 0.2840 18.842 0.3768 24.998 

 3537 3 M 1.6212 0.3290 20.294 0.4376 26.992 

 13 F 1.5736 0.2926 18.594 0.3384 21.505 

 3538 1 M 1.6451 0.2985 18.145 0.3570 21.701 

 11 F 1.5596 0.3369 21.602 0.3746 24.019 

 3539 5 M 1.6564 0.4276 25.815 0.3941 23.793 

 11 F 1.5593 0.2644 16.956 0.2913 18.681 

 3540 2 M 1.5612 0.3886 24.891 0.3800 24.340 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 505 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP III 10000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 3540 (continued)

 13 F 1.5592 0.3123 20.030 0.4793 30.740 

 3541 5 M 1.6801 0.4746 28.248 0.4214 25.082 

 14 F 1.5283 0.3730 24.406 0.4039 26.428 

 3542 1 M 1.7123 0.4146 24.213 0.4047 23.635 

 8 F 1.6336 0.2808 17.189 0.4060 24.853 

 3543 3 M 1.4558 0.2913 20.010 0.3286 22.572 

 9 F 1.4384 0.2647 18.402 0.3486 24.235 

 3544 2 M 1.4253 0.4062 28.499 0.3154 22.129 

 10 F 1.3822 0.3317 23.998 0.3384 24.483 

 3545 3 M 1.6104 0.3438 21.349 0.3912 24.292 

 13 F 1.5311 0.2695 17.602 0.3564 23.277 

 3546 6 M 1.6226 0.4419 27.234 0.3793 23.376 

 10 F 1.5126 0.3273 21.638 0.3550 23.470 

 3547 6 M 1.4517 0.3239 22.312 0.3827 26.362 

 7 F 1.3869 0.3343 24.104 0.3582 25.827 

 3548 2 M 1.5685 0.3662 23.347 0.5282 33.675 

 9 F 1.5315 0.2989 19.517 0.4246 27.724 

 3549 2 M 1.6042 0.4282 26.692 0.4164 25.957 

 8 F 1.5253 0.3449 22.612 0.4253 27.883 

 3550 7 M 1.5579 0.2227 14.295 0.2234 14.340 

 13 F 1.5527 0.3180 20.480 0.3543 22.818 

 3551 5 M 1.6458 0.3949 23.994 0.4535 27.555 

 14 F 1.4841 0.2593 17.472 0.3728 25.120 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 506 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 4526 6 M 1.5900 0.2610 16.415 0.3437 21.616 

 13 F 1.5093 0.2536 16.802 0.2959 19.605 

 4527 2 M 1.6845 0.2989 17.744 0.3947 23.431 

 10 F 1.5719 0.2577 16.394 0.3715 23.634 

 4528 5 M 1.5686 0.3223 20.547 0.3162 20.158 

 11 F 1.4889 0.2670 17.933 0.3539 23.769 

 4529 3 M 1.6241 0.3153 19.414 0.2591 15.953 

 15 F 1.5165 0.2907 19.169 0.3411 22.493 

 4530 3 M 1.5800 0.2314 14.646 0.2733 17.297 

 7 F 1.4575 0.2425 16.638 0.3562 24.439 

 4531 3 M 1.5429 0.3105 20.124 0.3014 19.535 

 9 F 1.4720 0.3094 21.019 0.3167 21.515 

 4532 8 M 1.6124 0.4105 25.459 0.4977 30.867 

 11 F 1.5440 0.3080 19.948 0.3749 24.281 

 4533 6 M 1.4033 0.3446 24.556 0.3006 21.421 

 9 F 1.4734 0.3914 26.564 0.2759 18.725 

 4534 4 M 1.6063 0.3910 24.342 0.3234 20.133 

 12 F 1.5083 0.2976 19.731 0.3687 24.445 

 4535 6 M 1.5830 0.2355 14.877 0.3228 20.392 

 15 F 1.5242 0.2357 15.464 0.3881 25.463 

 4536 6 M 1.6077 0.3940 24.507 0.4485 27.897 

 9 F 1.5048 0.2562 17.026 0.3505 23.292 

 4537 2 M 1.5821 0.2514 15.890 0.2224 14.057 
11-JAN-2010 18:17

Huntingdon Life Sciences 00-4205 PAGE 507 

 

 APPENDIX Y F1 GENERATION 

 GASOLINE DIPE VAPOR CONDENSATE: A ONE-GENERATION WHOLE-BODY 

 INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

 INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) . 

GROUP IV 20000 MG/M3 

-----------------------------------------------------------------------------------------------------------------------------------

 Final BRAIN SPLEEN THYMUS 

 Animal Pup Body -------------- -------------- -------------- 

 Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio 

-----------------------------------------------------------------------------------------------------------------------------------

 4537 (continued)

 9 F 1.5349 0.3574 23.285 0.3401 22.158 

 4539 3 M 1.6141 0.2813 17.428 0.2915 18.060 

 8 F 1.5236 0.3069 20.143 0.4142 27.186 

 4540 6 M 1.5921 0.3782 23.755 0.4326 27.172 

 12 F 1.5358 0.3555 23.148 0.4391 28.591 

 4541 3 M 1.5188 0.3050 20.082 0.2143 14.110 

 12 F 1.4771 0.3549 24.027 0.3694 25.008 

 4542 2 M 1.5190 0.3666 24.134 0.5598 36.853 

 7 F 1.5489 0.2422 15.637 0.4369 28.207 

 4545 6 M 1.5021 0.3198 21.290 0.4134 27.521 

 11 F 1.4356 0.2223 15.485 0.3621 25.223 

 4546 4 M 1.5617 0.3026 19.376 0.3637 23.289 

 10 F 1.4631 0.2413 16.492 0.3731 25.501 

 4547 2 M 1.5124 0.3743 24.749 0.3504 23.168 

 13 F 1.5273 0.2870 18.791 0.3658 23.951 

 4548 1 M 1.4496 0.2164 14.928 0.2596 17.908 

 12 F 1.5342 0.2643 17.227 0.3383 22.051 

 4549 6 M 1.5552 0.2918 18.763 0.3282 21.103 

 10 F 1.5510 0.2864 18.466 0.2909 18.756 

 4550 8 M 1.5266 0.2512 16.455 0.3516 23.032 

 12 F 1.5166 0.2872 18.937 0.3565 23.507 
Huntingdon Life Sciences 00-4205 Page 508

211-DIPE-1G Final Report

Individual Animal Gross

and Microscopic Observations

Preface Appendix Z

Key to Abbreviations

Gl = Gland

Oviducts/Fallop = Oviducts/Fallopian

Notes

1. Unless otherwise specified in a histopathology note, the organ/tissue 

examined was the required (routine) section.

2. The dimensions of gross lesions are presented as a general range based on 

the actual dimensions noted during macroscopic examinations. The actual 

dimensions of gross lesions can be found in the raw data. 

3. For summarization purposes, descriptive comments [i.e., location of scab(s) 

and/or sore(s), etc.] are not presented in this appendix. These data are 

contained in the study raw data if needed.

Corresponding exposure levels for each group were as follows:

Group 1 - 0 mg/m3 

Group 2 - 2,000 mg/m3

Group 3 - 10,000 mg/m3

Group 4 - 20,000 mg/m3









































AUDITED FINAL REPORT 

Study Phase: Sperm Analysis

Test Site Phase Reference Number 93075132 

Testing Facility Study Number 00-4205 

Sponsor Reference Number 211-DIPE-1G 

Gasoline DIPE Vapor Condensate: 

A One-Generation Whole-Body Inhalation Reproduction 

Toxicity Study in Rats 

TEST SITE: 

Charles River Laboratories, Pathology Associates - Maryland 

15 Worman’s Mill Court, Suite I 

Frederick, MD 21701 

TESTING FACILITY: 

Huntington Life Sciences (HLS) 

Mettlers Road 

P.O. Box 2360 

East Millstone, NJ 08875-2360 

SPONSOR: 

American Petroleum Institute (API) 

1220 L Street, Northwest 

Washington, D.C. 20005-4070 

09-APR-2009 

Page 1 of 14 

Appendix AA Page 550
Final Report 

HLS Study No.: 00-4205 

2

TABLE OF CONTENTS 

Section Page No.

I. QUALITY ASSURANCE STATEMENT ............................. 4

II. MATERIALS AND METHODS ................................... 5

A. Sample Collection................................... 5

B. Sperm Motility Evaluation........................... 5

C. Caudal Epididymis Weight and Total Sperm Count 

Determination ...................................... 6

D. Sperm Morphology Evaluation......................... 6

E. Statistical Analyses................................ 6

F. GLP Compliance Statement............................ 7

G. Sample and Data Archiving........................... 7

III. RESULTS ................................................. 7

A. Sperm Motility...................................... 7

B. Total Sperm Count................................... 7

C. Sperm Morphology.................................... 8

D. Caudal Epididymis Weight............................ 8

IV. DISCUSSION AND CONCLUSIONS .............................. 8

V. REFERENCES .............................................. 9

Page 551
Final Report 

HLS Study No.: 00-4205 

3

INDEX OF TABLES AND APPENDICES 

Table Page No.

 1. Summary of Male Reproductive Parameters ................ 10 

Appendix Page No.

A. Individual Caudal Epididymis Weight, 

 Sperm Motility and Sperm Count Data .................... 11 

B. Individual Sperm Morphology Data ........................ 13 

Page 552

Final Report 

HLS Study No.: 00-4205 

5

Gasoline DIPE Vapor Condensate: 

A One-Generation Whole-Body Inhalation Reproduction Toxicity 

Study in Rats 

 II. MATERIALS AND METHODS 

 A. Sample Collection

 All surviving male rats were euthanized by exsanguination 

following anesthesia with inhaled carbon dioxide after the last F1

litters were weaned. For all animals, the abdominal cavity was 

opened and the reproductive organs exposed. For motility 

assessment, the left vas deferens was dissected away from the 

testis and immediately placed in a petri dish containing 10 ml of 

a solution consisting of 1% Bovine Serum Albumin dissolved in 

Phosphate Buffered Saline. The solution was prewarmed to a 

temperature of approximately 38oC. A minimum 3-minute period was 

allowed for the sperm to swim out. 

 For total sperm count assessment, the left epididymis and 

left testis were then removed and placed on dry ice. The frozen 

epididymides and testes were then transferred to Pathology 

Associates, A Division of Charles River Laboratories, Inc. 

(formerly known as Pathology Associates International until 

January 8, 2001), Frederick, MD. and stored frozen at -70oC until 

evaluation for caudal epididymal sperm count and homogenizationresistant testicular spermatid count. 

 Only the control and high-exposure animals were examined for 

percent motility, total epididymal sperm, homogenization-resistant 

testicular spermatid count, sperm morphology and caudal epididymis 

weight. 

 B. Sperm Motility Evaluation

 Following the swim out period, a sperm sample was obtained 

using a 100 μm deep cannula. The cannula was immediately loaded 

into the prewarmed stage of the Hamilton Thorne IVOS (Ver. 12.1c) 

automated sperm analyzer. The analyzer automatically selected 

five fields and each motion image was digitally saved and 

permanently stored on optical media. The images were 

subsequently analyzed and the percent motility determined for 

each animal. 

Page 554
Final Report 

HLS Study No.: 00-4205 

6

 C. Caudal Epididymis Weight and Total Sperm Count Determination

 Each frozen epididymis was removed from the freezer, thawed 

and the caudal section was trimmed and weighed. Each frozen 

testis was removed from the freezer, thawed, the tunica removed 

and the testicular parenchyma weighed. The cauda epididymis and 

testicular parenchyma were homogenized in deionized water and the 

suspensions were transferred to plastic test tubes and vortexed. A 

100 μl sample was transferred to a violet reaction vial containing 

a Hoechst dye (H33342) which uniquely stains the head of the 

sperm. A sample of the stained sperm was placed into a 20 μm deep 

Cell-Vu glass slide which was loaded into the Hamilton Thorne IVOS 

(Ver. 12.1c) analyzer. Twenty fields were automatically selected 

by the analyzer for each animal and the number of homogenizationresistant spermatids and cauda epididymal sperm determined. The 

counts reported were adjusted for caudal epididymal or testicular 

weight. 

 D. Sperm Morphology Evaluation

 Two Eosin stained slides were prepared for each animal from 

the caudal epididymis total count preparation. The slides were 

evaluated and a minimum of 200 sperm cells/animal was examined for 

morphological development. 

 E. Statistical Analyses

 The means and standard deviations for the sperm motility, 

caudal epididymal sperm count, testicular homogenization-resistant 

spermatid count and sperm morphology data were calculated and 

compared across groups using the Kruskal-Wallis nonparametric 

ANOVA test. If a significant effect occurred (p<0.05), the MannWhitney U test was used for pair-wise comparisons of each treated 

group to the control group. Animals with fewer than 25 sperm 

cells present in the motility analysis were excluded from the 

group mean calculation and subsequent statistical analysis. 

Animals with at least 25 sperm cells were included in the group 

mean calculation subsequent and statistical analysis; however, 

animals with fewer than 50 cells were identified to have a low 

count. The mean caudal epididymis weight for each group was 

calculated and compared using one-way analysis of variance (ANOVA) 

technique. If ANOVA is significant, Dunnett's test was used for 

pair-wise comparisons of each treated group to the control group 

at the 5% and 1% risk levels. Statistical analyses were 

performed using an IBMTM compatible computer with SAS computer 

programs (SAS/STAT User's Guide, 1989). 

Page 555
Final Report 

HLS Study No.: 00-4205 

7

 F. GLP Compliance Statement

 The portion of this study performed by PAI was conducted in 

compliance with US EPA 79.60, CFR Vol. 59, No. 122, 27 June 1994. 

 G. Sample and Data Archiving

 Frozen epididymis and testis samples not processed for sperm 

count determination were transferred to and stored frozen at -70oC 

in PAI's long-term archive facility. The optical media used for 

permanent storage of the sperm motility images, raw data printouts 

from the sperm motility, epididymal and testicular sperm count and 

sperm morphology analysis, as well as any supporting documentation 

and the sperm morphology slides will be maintained at PAI until 

the study has been completed. Upon finalizing the report, all 

non-frozen study material will be returned to HLS for archiving. 

Disposition of the frozen tissue samples will be directed by 

correspondence from HLS. 

 III. RESULTS 

 A. Sperm Motility

 Table 1 (Summary Data) 

 Appendix A (Individual Data) 

 No apparent treatment-related effects were observed in the 

sperm motility data. Group mean values were 90% and 93% for the 

control and high-exposure groups, respectively. There was one 

animal each in the control (animal 1026) and high-exposure (animal 

4026) group for which sperm motility images were not recorded due 

to a technical problem with the IVOS sperm analyzer. There was 

one animal (animal 1048) in the control group with no motile 

sperm. Animal 1034 in the control group had fewer than 25 sperm 

cells counted and was excluded from the group mean. 

 B. Total Sperm Count

 Table 1 (Summary Data) 

 Appendix A (Individual Data) 

 The number of sperm per gram of caudal epididymis and the 

number of spermatids per gram of testis were comparable between 

the control and high-exposure groups. Group mean values were 831.6 

and 927.9 million sperm/gram for the epididymal sperm count and 

101.2 and 103.9 million sperm/gram for the homogenizationresistant testicular count. 

Page 556

Final Report 

HLS Study No.: 00-4205 

9

 V. REFERENCES 

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV 

functions for calculating probabilities associated with 

Dunnett’s test. Behav. Res. Methods and Instrumentation 13: 

363-366. 

Dunnett, C. W. 1955. A multiple comparison procedure for 

comparing several treatments with a control. Journal of the 

American Statistical Association 50:1096-1121. 

Dunnett, C. W. 1964. New tables for multiple comparisons with 

a control. Biometrics 20:482-491. 

Kruskal, W.H. and Wallis, W.A. 1952. Use of ranks in onecriterion variance analysis. Journal of the American 

Statistical Association 47: 583-621. 

Kruskal, W.H. and Wallis, W.A. 1953. Errata for KruskalWallis (1952). Journal of the American Statistical 

Association 48: 907-911. 

Kruskal, W.H. 1957. Historical notes on the Wilcoxon unpaired 

two-sample test. Journal of the American Statistical 

Association 52: 356-360. 

SAS/STAT User's Guide, 1989a. SAS Institute Inc., Version 6, 

Fourth Edition, Cary, NC:SAS Institute Inc., 1989. Vol. 1, 

p. 209-244. Vol. 2 p. 1195-1210. 

Page 558
10

HLS STUDY NO.: 00-4205 

TABLE 1 

GASOLINE DIPE VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

SUMMARY OF MALE REPRODUCTIVE PARAMETERS 

Group: I IV 

Exposure Level: GDIPEVCa (mg/m3) 0 20,000 

MOTILITY (%) 

 Mean 90 93 

 SD 20 6 

 N 24 25 

EPIDIDYMAL COUNT 

 (MILLION SPERM/GRAM) 

 Mean 831.6 927.9 

 SD 189.1 177.2 

 N 26 26 

TESTICULAR COUNT 

 (MILLION SPERM/GRAM) 

 Mean 101.2 103.9 

 SD 16.1 12.0 

 N 26 26 

SPERM MORPHOLOGYb

 (% ABNORMAL) 

 Mean 7.4 0.4*

 SD 23.5 0.5 

 N 26 26 

CAUDAL EPIDIDYMIS WEIGHTS 

 (GRAM) 

 Mean 0.26 0.27*

 SD 0.03 0.02 

 N 26 26 

aGASOLINE DIPE VAPOR CONDENSATE. 

bMEAN AND STANDARD DEVIATIONS WERE CALCULATED USING THE TOTAL NUMBER OF ABNORMAL SPERM 

AS A PERCENTAGE OF THE NUMBER OF SPERM EXAMINED. 

*SIGNIFICANTLY DIFFERENT FROM CONTROL GROUP (p<0.05). 

Page 559
11

HLS STUDY NO.: 00-4205 

APPENDIX A 

GASOLINE DIPE VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT, 

SPERM MOTILITY AND SPERM COUNT DATA 

Group I: 0 mg/m3 (GASOLINE DIPE VAPOR CONDENSATE) 

S P E R M C O U N T 

Animal Caudal Motility Caudal Homogenization Resistant 

 No. Epididymis Epididymal Testicular 

Weight (g) (%) (million sperm/gram) 

1026 0.21 a 956.0 105.2 

1027 0.30 94 794.7 104.6 

1028 0.26 97 764.1 109.9 

1029 0.27 95 650.6 108.1 

1030 0.29 99 973.5 107.1 

1031 0.19 87 1012.6 132.1 

1032 0.28 91 952.3 96.6 

1033 0.25 98 639.9 81.8 

1034 0.23 0

b 363.7 62.1 

1035 0.27 93 832.6 89.1 

1036 0.27 87 677.7 104.0 

1037 0.22 96 945.8 123.6 

1038 0.26 95 631.4 105.3 

1039 0.21 97 1080.5 101.0 

1040 0.29 99 894.2 94.4 

1041 0.27 96 855.9 92.1 

1042 0.22 85 632.1 101.5 

1043 0.26 78 876.7 99.8 

1044 0.23 94 1113.8 100.2 

1045 0.24 99 975.9 105.2 

1046 0.28 96 1116.6 71.7 

1047 0.24 94 758.1 107.2 

1048 0.29 0

c 501.2 131.7 

1049 0.32 88 937.8 77.6 

1050 0.28 98 952.3 113.8 

1051 0.23 98 731.9 104.6 

aTECHNICAL ERROR; SPERM MOTILITY IMAGES WERE NOT SAVED. 

b

FEWER THAN 25 SPERM CELLS COUNTED IN 5 FIELDS ANALYZED; EXCLUDED FROM 

STATISTICAL ANALYSIS. c

FEWER THAN 50 SPERM CELLS COUNTED IN 5 FIELDS ANALYZED. 

Page 560
12

HLS STUDY NO.: 00-4205 

APPENDIX A 

GASOLINE DIPE VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT, 

SPERM MOTILITY AND SPERM COUNT DATA 

Group IV: 20,000 mg/m3 (GASOLINE DIPE VAPOR CONDENSATE) 

S P E R M C O U N T 

Animal Caudal Motility Caudal Homogenization Resistant 

 No. Epididymis Epididymal Testicular 

Weight (g) (%) (million sperm/gram) 

4026 0.26 a 896.8 109.9 

4027 0.30 94 798.2 101.7 

4028 0.26 89 929.0 102.8 

4029 0.30 97 885.3 96.9 

4030 0.25 92 807.2 86.1 

4031 0.27 79 542.2 113.4 

4032 0.26 90 969.2 100.6 

4033 0.30 99 864.4 121.4 

4034 0.26 94 916.9 125.5 

4035 0.30 76 798.2 118.5 

4036 0.25 94 924.3 107.1 

4037 0.30 88 1017.8 77.8 

4038 0.27 98 747.4 89.5 

4039 0.30 98 996.8 87.2 

4040 0.27 99 844.3 116.4 

4041 0.26 96 925.0 93.9 

4042 0.25 97 1535.0 109.2 

4043 0.29 97 1139.4 100.4 

4044 0.25 93 1012.2 99.6 

4045 0.34 98 1125.6 94.2 

4046 0.25 96 836.5 117.1 

4047 0.26 85 1065.7 91.0 

4048 0.27 92 848.1 113.5 

4049 0.26 98 756.1 106.7 

4050 0.25 96 974.5 107.4 

4051 0.29 95 969.9 112.4 

aTECHNICAL ERROR; SPERM MOTILITY IMAGES WERE NOT SAVED. 

Page 561
HLS STUDY NO.: 00-4205 

APPENDIX B 

GASOLINE DIPE VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL SPERM MORPHOLOGY DATA 

13

Group I: 0 mg/m3 (GASOLINE DIPE VAPOR CONDENSATE) 

Animal ----------------H e a d---------------- --------T a i l----

 No. Normal Amorphous Small Enlarged Double Coiled Bent D

1026 200 0 0 0 0 0 0 

1027 199 1 0 0 0 0 0 

1028 197 3 0 0 0 0 0 

1029 200 0 0 0 0 0 0 

1030 199 0 1 0 0 0 0 

1031 200 0 0 0 0 0 0 

1032 197 2 1 0 0 0 0 

1033 198 2 0 0 0 0 0 

1034 19 181 0 0 0 0 0 1035 199 1 0 0 0 0 0 

1036 197 3 0 0 0 0 0 

1037 200 0 0 0 0 0 0 

1038 200 0 0 0 0 0 0 

1039 196 2 2 0 0 0 0 

1040 199 1 0 0 0 0 0 

1041 200 0 0 0 0 0 0 

1042 197 3 0 0 0 0 0 

1043 200 0 0 0 0 0 0 

1044 196 4 0 0 0 0 0 

1045 197 2 1 0 0 0 0 

1046 200 0 0 0 0 0 0 

1047 199 1 0 0 0 0 0 

1048 32 168 0 0 0 0 0 1049 194 6 0 0 0 0 0 

1050 200 0 0 0 0 0 0 

1051 199 0 1 0 0 0 0 
HLS STUDY NO.: 00-4205 

APPENDIX B 

GASOLINE DIPE VAPOR CONDENSATE: 

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS 

INDIVIDUAL SPERM MORPHOLOGY DATA 

14

Group IV: 20,000 mg/m3 (GASOLINE DIPE VAPOR CONDENSATE) 

Animal ----------------H e a d---------------- --------T a i l----

 No. Normal Amorphous Small Enlarged Double Coiled Bent D

4026 200 0 0 0 0 0 0 

4027 199 1 0 0 0 0 0 

4028 200 0 0 0 0 0 0 

4029 198 2 0 0 0 0 0 

4030 199 0 1 0 0 0 0 

4031 199 1 0 0 0 0 0 

4032 200 0 0 0 0 0 0 

4033 200 0 0 0 0 0 0 

4034 199 1 0 0 0 0 0 

4035 200 0 0 0 0 0 0 

4036 198 2 0 0 0 0 0 

4037 200 0 0 0 0 0 0 

4038 200 0 0 0 0 0 0 

4039 200 0 0 0 0 0 0 

4040 199 1 0 0 0 0 0 

4041 200 0 0 0 0 0 0 

4042 199 1 0 0 0 0 0 

4043 198 1 1 0 0 0 0 

4044 200 0 0 0 0 0 0 

4045 200 0 0 0 0 0 0 

4046 199 1 0 0 0 0 0 

4047 200 0 0 0 0 0 0 

4048 196 4 0 0 0 0 0 

4049 198 2 0 0 0 0 0 

4050 200 0 0 0 0 0 0 

4051 200 0 0 0 0 0 0 
Huntingdon Life Sciences 00-4205 Page 564 

 211-DIPE-1G Final Report

 

Certificates of Analysis Appendix BB

1. Feed Certificates of Analysis ....................................................................... 565 

2. Water Certificates of Analysis ..................................................................... 579 

3. Bedding Certificates of Analysis .................................................................. 591 
Page 565
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Appendix CC

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Huntingdon Life Sciences 00-4205 Page 630 

 211-DIPE-1G Final Report

 Historical Control Data 

Preface Appendix DD 

CD Rat - Mating, Fertility and Gestation ........................................................... 631 

CD Rat - Summary of Mean Pup Organ Weights ............................................. 632 
Huntingdon Life Sciences 00-4205 

 211-DIPE-1G 

Historical Control Data 

CD Rat - Mating, Fertility and Gestation Ap

Females 

Study Initiation Paired w/ Mated 

Mating 

Index Pregnant 

Fertility 

Index 

No. w

Liveb

ID Date Males No. No. % No. % No

00-4202 22-Aug-01 26 26 100 24 92.3 200-4203 21-Jan-02 26 26 100 25 96.2 200-4204 19-Mar-02 26 26 100 25 96.2 200-4206 20-Aug-02 26 24 92.3 21 87.5 2

00-4207 P0 30-Apr-01 26 25 96.2 24 96 2

00-4207 F1 26 25 96.2 25 100 2

00-4208 P0 12-Jun-01 26 24 92.3 23 95.8 2

00-4208 F1 26 23 88.5 21 91.3 2

 MEAN 25 95.7 24 94.4 2

SD 1 4.3 2 3.9 2 

N 8 8 8 8 8 

 MIN 23 88.5 21 87.5 2

 MAX 26 100.0 25 100.0 2
Huntingdon Life Sciences 00-4205 

 211-DIPE-1G 

Males 

Historical Control Data 

CD Rat - Summary of Mean Pup Organ Weights Ap

TBW=Terminal Body Weight; ORG=Organ Weight 

Study TBW Brain Spleen Thym

ID Wt. ORG/TBW WT ORG/TBW ORG/BRAIN WT ORG/TB

 (g) (g) (%) (g) (%) (%) (g) (%) 

00-4202 Mean 82.3 1.5825 1.9312 0.3245 0.3951 20.514 0.3586 0.4 S.D. 6.4 0.0629 0.1263 0.0500 0.0588 3.142 0.0547 0.0 N 22 22 22 22 22 22 22 00-4203 Mean 81.8 1.6118 1.9785 0.3445 0.4210 21.381 0.3673 0.4 S.D. 6.8 0.0713 0.1305 0.0477 0.0472 2.845 0.0887 0.1 N 25 25 25 25 25 25 25 00-4204 Mean 77.3 1.5633 2.0568 0.3173 0.4135 20.303 0.3514 0.4 S.D. 11.8 0.0905 0.2632 0.0517 0.0550 3.196 0.0771 0.0 N 13 13 13 13 13 13 13 00-4206 Mean 80.2 1.5485 1.9522 0.3380 0.4231 21.822 0.3570 0.4 S.D. 9.8 0.0990 0.2116 0.0515 0.0528 3.068 0.0691 0.0 N 20 20 20 20 20 20 20 00-4207 Mean 72.7 1.5939 2.2127 0.2792 0.3850 17.589 0.3238 0.4 S.D. 8.1 0.1572 0.2845 0.0491 0.0613 3.031 0.0788 0.1 N 23 23 23 23 23 23 23 00-4208 Mean 75.6 1.5634 2.0757 0.3218 0.4258 20.582 0.3322 0.4 S.D. 5.6 0.0591 0.1267 0.0484 0.0589 3.031 0.0600 0.0 N 22 22 22 22 22 22 22 Overall Mean 78.4 1.5797 2.0343 0.3211 0.4100 20.349 0.3483 0.4 S.D. 8.6 0.0970 0.2157 0.0533 0.0571 3.293 0.0729 0.0 N 125 125 125 125 125 125 125 
Huntingdon Life Sciences 00-4205 

 211-DIPE-1G 

Females 

Historical Control Data 

CD Rat - Summary of Mean Pup Organ Weights Ap

TBW=Terminal Body Weight; ORG=Organ Weight 

Study TBW Brain Spleen Thym

ID Wt. ORG/TBW WT ORG/TBW ORG/BRAIN WT ORG/TB

 (g) (g) (%) (g) (%) (%) (g) (%) 

00-4202 Mean 76.7 1.5323 2.0070 0.2884 0.3741 18.875 0.3836 0.5 S.D. 5.5 0.0671 0.1472 0.0681 0.0702 4.548 0.0468 0.0 N 22 22 22 22 22 22 22 00-4203 Mean 75.1 1.5506 2.0750 0.3043 0.4041 19.597 0.3459 0.4 S.D. 6.4 0.0631 0.1576 0.0559 0.0606 3.307 0.0466 0.1 N 25 25 25 25 25 25 25 00-4204 Mean 68.4 1.4423 2.1509 0.2905 0.4296 20.168 0.3468 0.5 S.D. 11.5 0.1499 0.3410 0.0588 0.0771 3.510 0.0808 0.0 N 13 13 13 13 13 13 13 00-4206 Mean 73.9 1.5185 2.0712 0.3023 0.4107 19.934 0.3484 0.4 S.D. 7.0 0.0805 0.1894 0.0440 0.0545 2.928 0.0588 0.0 N 20 20 20 20 20 20 20 00-4207 Mean 67.4 1.5015 2.2454 0.2440 0.3613 16.388 0.3253 0.4 S.D. 6.9 0.1191 0.2698 0.0473 0.0564 3.713 0.0884 0.1 N 23 23 23 23 23 23 23 00-4208 Mean 69.9 1.5284 2.1971 0.2831 0.4058 18.506 0.3420 0.4 S.D. 5.9 0.0688 0.1724 0.0428 0.0573 2.541 0.0607 0.0 N 22 22 22 22 22 22 22 Overall Mean 72.2 1.5181 2.1232 0.2849 0.3950 18.8008 0.3485 0.4 S.D. 7.7 0.0946 0.2243 0.0563 0.0647 3.6457 0.0655 0.0 N 125 125 125 125 125 125 125 
Huntingdon Life Sciences 00-4205 

 211-DIPE-1G 

Combined 

Historical Control Data 

CD Rat - Summary of Mean Pup Organ Weights Ap

TBW=Terminal Body Weight; ORG=Organ Weight 

Study TBW Brain Spleen Thym

ID Wt. ORG/TBW WT ORG/TBW ORG/BRAIN WT ORG/TB

 (g) (g) (%) (g) (%) (%) (g) (%) 

00-4202 Mean 79.5 1.5574 1.9691 0.3064 0.3846 19.694 0.3711 0.4 S.D. 4.9 0.0538 0.1141 0.0498 0.0494 3.371 0.0405 0.0 N 22 22 22 22 22 22 22 00-4203 Mean 78.5 1.5812 2.0267 0.3244 0.4120 20.489 0.3566 0.4 S.D. 6.0 0.0561 0.1306 0.0467 0.0465 2.769 0.0607 0.0 N 25 25 25 25 25 25 25 00-4204 Mean 72.9 1.5028 2.1039 0.3039 0.4216 20.235 0.3491 0.4 S.D. 11.2 0.0909 0.2906 0.0526 0.0621 3.167 0.0701 0.0 N 13 13 13 13 13 13 13 00-4206 Mean 77.0 1.5335 2.0117 0.3202 0.4169 20.878 0.3527 0.4 S.D. 7.5 0.0829 0.1836 0.0374 0.0435 2.398 0.0563 0.0 N 20 20 20 20 20 20 20 00-4207 Mean 70.1 1.5477 2.2290 0.2616 0.3731 16.989 0.3246 0.4 S.D. 6.9 0.1082 0.2357 0.0423 0.0525 2.746 0.0732 0.1 N 23 23 23 23 23 23 23 00-4208 Mean 72.8 1.5459 2.1364 0.3024 0.4158 19.544 0.3371 0.4 S.D. 4.8 0.0504 0.1348 0.0391 0.0510 2.453 0.0501 0.0 N 22 22 22 22 22 22 22 Overall Mean 75.3 1.5489 2.0787 0.3030 0.4025 19.575 0.3484 0.4 S.D. 7.5 0.0771 0.2001 0.0486 0.0526 3.057 0.0597 0.0 N 125 125 125 125 125 125 125 
Huntingdon Life Sciences 00-4205 Page 635 

 211-DIPE-1G Final Report 

 

Testing Facility Personnel Appendix EE 

TITLE/DEPARTMENT NAME/DEGREE 

SENIOR VICE PRESIDENT, 

 SAFETY ASSESSMENT 

Sylvie J. Gosselin, D.V.M., Ph.D., 

 Diplomate A.C.V.P. 

 

SENIOR DIRECTOR, SAFETY 

 ASSESSMENT 

Carol S. Auletta, M.B.A., D.A.B.T., 

 R.A.C. 

 

DIRECTOR, TOXICOLOGY 

 OPERATIONS Ian Vanterpool, F.I.A.T. 

 

DIRECTOR, PATHOLOGY SERVICES Barbara A. Litzenberger, B.S., 

 M.T. (ASCP) 

 

DIRECTOR, QUALITY ASSURANCE Nicki S. Iacono, B.S. 

STUDY DIRECTOR Gary M. Hoffman, B.A., D.A.B.T. 

PATHOLOGIST Katharine M. Whitney, D.V.M., 

 Ph.D., Diplomate A.C.V.P. 

 

STUDY MONITOR Ronald L. Brzozowski, B.A., 

 AALAS LAT 

 

VETERINARIAN Teresa S. Kusznir, V.M.D. 

MANAGER/SUPERVISOR 

 Rodent/Inhalation Toxicology and 

Pharmacy 

 Reproductive Toxicology 

 Analytical Services 

 Histology

Ellen H. Whiting, AALAS LAT 

 AALAS LAT 

Robert A. Faust, B.S. 

Kay Saladdin, B.S. 

Janet Kusisto 
Huntingdon Life Sciences 00-4205 Page 636 

 211-DIPE-1G Final Report

 

Report Amendments Appendix FF 

There are no amendments for this report at this time.