# Patent

## Title
Methods for making Apo-2 ligand using divalent metal ions

## Abstract

Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.

## Background
RELATED APPLICATIONS
This is a continuation application claiming priority to U.S. application Ser. No. 09/603,866 filed Jun. 26, 2000, now abandoned, which is a non-provisional application claiming priority under Section 119(e) to provisional application No. 60/141,342 filed Jun. 28, 1999, the contents of which are incorporated herein by reference.


FIELD OF THE INVENTION
The present invention relates generally to making Apo-2 ligand and Apo-2 ligand formulations using divalent metal ions, such as zinc or cobalt. The use of such Apo-2 ligand and Apo-2 ligand formulations having improved Apo-2L trimer formation and stability is also provided. The present invention also relates to Apo-2 ligand variants, particularly alanine substitution variants.
BACKGROUND OF THE INVENTION
Control of cell numbers in mammals is believed to be determined, in part, by a balance between cell proliferation and cell death. One form of cell death, sometimes referred to as necrotic cell death, is typically characterized as a pathologic form of cell death resulting from some trauma or cellular injury. In contrast, there is another, “physiologic” form of cell death which usually proceeds in an orderly or controlled manner. This orderly or controlled form of cell death is often referred to as “apoptosis” [see, e.g., Barr et al., Bio/Technology, 12:487-493 (1994); Steller et al., Science, 267:1445-1449 (1995)]. Apoptotic cell death naturally occurs in many physiological processes, including embryonic development and clonal selection in the immune system [Itoh et al., Cell, 66:233-243 (1991)].
Various molecules, such as tumor necrosis factor-α (“TNF-α”), tumor necrosis factor-β (“TNF-β” or “lymphotoxin-α”), lymphotoxin-β (“LT-β”), CD30 ligand, CD27 ligand, CD40 ligand, OX-40 ligand, 4-1BB ligand, Apo-1 ligand (also referred to as Fas ligand or CD95 ligand), Apo-2 ligand (also referred to as TRAIL, AIM-1 or AGP-1), and Apo-3 ligand (also referred to as TWEAK) have been identified as members of the tumor necrosis factor (“TNF”) family of cytokines [See, e.g., Gruss and Dower, Blood, 85:3378-3404 (1995); Pitti et al., J. Biol. Chem., 271:12687-12690 (1996); Wiley et al., Immunity, 3:673-682 (1995); Browning et al., Cell, 72:847-856 (1993); Armitage et al. Nature, 357:8.0-82 (1992), WO 97/01633 published Jan. 16, 1997; WO 97/25428 published Jul. 17, 1997; WO 97/46686 published Dec. 11, 1997; WO 97/33899 published Sep. 18, 1997; Marsters et al., Curr. Biol., 8:525-528 (1998); Chicheportiche et al., Biol. Chem., 272:32401-32410 (1997)]. Among these molecules, TNF-α, TNF-β, CD30 ligand, 4-1BB ligand, Apo-1 ligand, Apo-2 ligand (TRAIL) and Apo-3 ligand (TWEAK) have been reported to be involved in apoptotic cell death.
Induction of various cellular responses mediated by such TNF family cytokines is believed to be initiated by their binding to specific cell receptors. Two distinct TNF receptors of approximately 55-kDa (TNFR1) and 75-kDa (TNFR2) have been identified [Hohman et al., J. Biol. Chem., 264:14927-14934 (1989); Brockhaus et al., Proc. Natl. Acad. Sci., 87:3127-3131 (1990); EP 417,563, published Mar. 20, 1991] and human and mouse cDNAs corresponding to both receptor types have been isolated and characterized [Loetscher et al., Cell, 61:351 (1990); Schall et al., Cell, 61:361 (1990); Smith et al., Science, 248:1019-1023 (1990); Lewis et al., Proc. Natl. Acad. Sci., 88:2830-2834 (1991); Goodwin et al., Mol. Cell. Biol., 11:3020-3026 (1991)]. Extensive polymorphisms have been associated with both TNF receptor genes [see, e.g., Takao et al., Immunogenetics, 37:199-203 (1993)]. Both TNFRs share the typical structure of cell surface receptors including extracellular, transmembrane and intracellular regions. The extracellular portions of both receptors are found naturally also as soluble TNF-binding proteins [Nophar, Y. et al., EMBO J., 9:3269 (1990); and Kohno, T. et al., Proc. Natl. Acad. Sci. U.S.A., 87:8331 (1990)]. The cloning of recombinant soluble TNF receptors was reported by Hale et al. [J. Cell. Biochem. Supplement 15F, 1991, p. 113 (P424)].
The extracellular portion of type 1 and type 2 TNFRs (TNFR1 and TNFR2) contains a repetitive amino acid sequence pattern of four cysteine-rich domains (CRDs) designated 1 through 4, starting from the NH2-terminus. Each CRD is about 40 amino acids long and contains 4 to 6 cysteine residues at positions which are well conserved [Schall et al., supra; Loetscher et al., supra; Smith et al., supra; Nophar et al., supra; Kohno et al., supra]. In TNFR1, the approximate boundaries of the four CRDs are as follows: CRD1—amino acids 14 to about 53; CRD2—amino acids from about 54 to about 97; CRD3—amino acids from about 98 to about 138; CRD4—amino acids from about 139 to about 167. In TNFR2, CRD1 includes amino acids 17 to about 54; CRD2—amino acids from about 55 to about 97; CRD3—amino acids from about 98 to about 140; and CRD4—amino acids from about 141 to about 179 [Banner et al., Cell, 73:431-435 (1993)]. The potential role of the CRDs in ligand binding is also described by Banner et al., supra.
A similar repetitive pattern of CRDs exists in several other cell-surface proteins, including the p75 nerve growth factor receptor (NGFR) [Johnson et al., Cell, 47:545 (1986); Radeke et al., Nature, 325:593 (1987)], the B cell antigen CD40 [Stamenkovic et al., EMBO J., 8:1403 (1989)], the T cell antigen OX40 [Mallet et al., EMBO J., 9:1063 (1990)] and the Fas antigen [Yonehara et al., J. Exp. Med., 169:1747-1756 (1989) and Itoh et al., Cell, 66:233-243 (1991)]. CRDs are also found in the soluble TNFR (sTNFR)-like T2 proteins of the Shope and myxoma poxviruses [Upton et al., Virology, 160:20-29 (1987); Smith et al., Biochem. Biophys. Res. Commun., 176:335 (1991); Upton et al., Virology, 184:370 (1991)]. Optimal alignment of these sequences indicates that the positions of the cysteine residues are well conserved. These receptors are sometimes collectively referred to as members of the TNF/NGF receptor superfamily. Recent studies on p75NGFR showed that the deletion of CRD1 [Welcher, A. A. et al., Proc. Natl. Acad. Sci. USA, 88:159-163 (1991)] or a 5-amino acid insertion in this domain [Yan, H. and Chao, M. V., J. Biol. Chem., 266:12099-12104 (1991)] had little or no effect on NGF binding [Yan, H. and Chao, M. V., supra]. p75 NGFR contains a proline-rich stretch of about 60 amino acids, between its CRD4 and transmembrane region, which is not involved in NGF binding [Peetre, C. et al., Eur. J. Hematol., 41:414-419 (1988); Seckinger, P. et al., J. Biol. Chem., 264:11966-11973 (1989); Yan, H. and Chao, M. V., supra]. A similar proline-rich region is found in TNFR2 but not in TNFR1.
The TNF family ligands identified to date, with the exception of lymphotoxin-α, are type II transmembrane proteins, whose C-terminus is extracellular. In contrast, most receptors in the TNF receptor (TNFR) family identified to date are type I transmembrane proteins. In both the TNF ligand and receptor families, however, homology identified between family members has been found mainly in the extracellular domain (“ECD”). Several of the TNF family cytokines, including TNF-α, Apo-1 ligand and CD40 ligand, are cleaved proteolytically at the cell surface; the resulting protein in each case typically forms a homotrimeric molecule that functions as a soluble cytokine. TNF receptor family proteins are also usually cleaved proteolytically to release soluble receptor ECDs that can function as inhibitors of the cognate cytokines.
Recently, other members of the TNFR family have been identified. Such newly identified members of the TNFR family include CAR1, HVEM and osteoprotegerin (OPG) [Brojatsch et al., Cell, 87:845-855 (1996); Montgomery et al., Cell, 87:427-436 (1996); Marsters et al., J. Biol. Chem., 272:14029-14032 (1997); Simonet et al., Cell, 89:309-319 (1997)]. Unlike other known TNFR-like molecules, Simonet et al., supra, report that OPG contains no hydrophobic transmembrane-spanning sequence. OPG is believed to act as a decoy receptor, as discussed below.
Another new member of the TNF/NGF receptor family has been identified in mouse, a receptor referred to as GITR for “glucocorticoid-induced tumor necrosis factor receptor family-related gene” [Nocentini et al., Proc. Natl. Acad. Sci. USA 94:6216-6221 (1997)]. The mouse GITR receptor is a 228 amino acid type I transmembrane protein that is expressed in normal mouse T lymphocytes from thymus, spleen and lymph nodes. Expression of the mouse GITR receptor was induced in T lymphocytes upon activation with anti-CD3 antibodies, Con A or phorbol 12-myristate 13-acetate.
In Marsters et al., Curr. Biol., 6:750 (1996), investigators describe a full length native sequence human polypeptide, called Apo-3, which exhibits similarity to the TNFR family in its extracellular cysteine-rich repeats and resembles TNFR1 and CD95 in that it contains a cytoplasmic death domain sequence [see also Marsters et al., Curr. Biol., 6:1669 (1996)]. Apo-3 has also been referred to by other investigators as DR3, wsl-1, TRAMP, and LARD [Chinnaiyan et al., Science, 274:990 (1996); Kitson et al., Nature, 384:372 (19961; Bodmer et al., Immunity, 6:79 (1997); Screaton et al., Proc. Natl. Acad. Sci., 94:4615-4619 (1997)].
Pan et al. have disclosed another TNF receptor family member referred to as “DR4” [Pan et al., Science, 276:111-113 (1997)]. The DR4 was reported to contain a cytoplasmic death domain capable of engaging the cell suicide apparatus. Pan et al. disclose that DR4 is believed to be a receptor for the ligand known as Apo-2 ligand or TRAIL.
In Sheridan et al., Science, 277:818-821 (1997) and Pan et al., Science, 277:815-818 (1997), another molecule believed to be a receptor for the Apo-2 ligand (TRAIL) is described. That molecule is referred to as DR5 (it has also been alternatively referred to as Apo-2; TRAIL-R2, TRICK2 or KILLER [Screaton et al., Curr. Biol., 7:693-696 (1997); Walczak et al., EMBO J., 16:5386-5387 (1997); Wu et al., Nature Genetics, 17:141-143 (1997)]. Like DR4, DR5 is reported to contain a cytoplasmic death domain and be capable of signaling apoptosis.
Yet another death domain-containing receptor, DR6, was recently identified [Pan et al., FEBS Letters, 431:351-356 (1998)]. Aside from containing four putative extracellular domains and a cytoplasmic death, domain, DR6 is believed to contain a putative leucine-zipper sequence that overlaps with a proline-rich motif in the cytoplasmic region. The proline-rich motif resembles sequences that bind to src-homology-3 domains, which are found in many intracellular signal-transducing molecules.
A further group of recently identified TNFR family members are referred to as “decoy receptors,” which are believed to function as inhibitors, rather than transducers of signaling. This group includes DCR1 (also referred to as TRID, LIT or TRAIL-R3) [Pan et al., Science, 276:111-113 (1997); Sheridan et al., Science, 277:818-821 (1997); McFarlane et al., J. Biol. Chem., 272:25417-25420 (1997); Schneider et al., FEBS Letters, 416:329-334 (1997); Degli-Esposti et al., J. Exp. Med., 186:1165-1170 (1997); and Mongkolsapaya et al., J. Immunol., 160:3-6 (1998)] and DCR2 (also called TRUNDD or TRAIL-R4) [Marsters et al., Curr. Biol., 7:1003-1006 (1997); Pan et al., FEBS Letters, 424:41-45 (1998); Degli-Esposti et al., Immunity, 7:813-820 (1997)], both cell surface molecules, as well as OPG [Simonet et al., supra] and DCR3 [Pitti et al., Nature, 396:699-703 (1998)], both of which are secreted, soluble proteins.
For a review of the TNF family of cytokines and their receptors, see Ashkenazi et al., Science, 281:1305-1308 (1998); Golstein, Curr. Biol., 7:750-753 (1997); and Gruss and Dower, supra.
While zinc binding sites have been shown to play structural roles in protein-protein interactions in certain proteins involving diverse interfaces [Feese et al., Proc. Natl. Acad. Sci., 91:3544-3548 (1994); Somers et al., Nature, 372:478-481 (1994); Raman et al., Cell, 95:939-950 (1998)], none of the previously structurally-characterized members of the TNF family (CD40 ligand, TNF-alpha, or TNF-beta) bind metals. The use of metal ions, such as zinc, in formulations of various hormones, such as human growth hormone (hGH), has been described in the literature. [See, e.g., WO 92/17200 published Oct. 15, 1992). Zinc involvement in hGH binding to receptors was likewise described in WO 92/03478 Published Mar. 5, 1992. The roles of zinc binding in interferon-alpha dimers and interferon-beta dimers were reported in Walter et al., Structure, 4:1453-1463 (1996) and Karpusas et al., Proc. Natl. Acad. Sci., 94:11813-11818 (1997), respectively.
The structures and biological roles of various metal ions such as zinc have been reviewed in the art, see, e.g., Christianson et al., Advances in Protein Chemistry, 42:281-355 (1991).
SUMMARY OF THE INVENTION
The present invention is based on the discovery that the inclusion of one or more divalent metal ions in methods or processes for making Apo-2 ligand, or formulations containing Apo-2 ligand, results in increased yield and stability of Apo-2 ligand trimers. It is presently believed that such inclusion of one or more divalent metal ions may also improve folding of Apo-2 ligand or Apo-2L trimer assembly upon expression in recombinant cell culture. In oxidative environments, free cysteines on Apo-2L monomers may form intermolecular disulfide bridges, giving rise to free-standing Apo-2L dimers as well as disulfide-linked Apo-2L dimer species within trimeric forms of Apo-2L. Such formation of Apo-2L dimers may lead to aggregation, precipitation, and/or inactivation of Apo-2L. The presence of divalent metal ions in the methods and formulations described herein may protect against such disulfide bond formation. It appears that inclusion of divalent metal ions during the process of synthesis and assembly of Apo-2L trimers may further improve accumulation and recovery of properly folded, homotrimeric Apo-2L. Applicants have found that Apo-2 ligand trimers are approximately 10-fold more active (in cytotoxic activity in mammalian cancer cells) as compared to disulfide-linked Apo-2L dimers.
While the description of the invention herein is primarily directed to Apo-2 ligand, the use of divalent metal ions to make or stabilize trimers of various other proteins is contemplated. Such other proteins particularly include those proteins which require trimer formation for biological activity, for instance, various members of the TNF family.
In one embodiment, the invention provides a method of making Apo-2 ligand using one or more divalent ions. The methods include the steps of providing a host cell comprising a replicable vector containing a nucleic acid encoding Apo-2 ligand, providing culture media containing one or more divalent metal ions, culturing the host cell in the culture media under conditions sufficient to express the Apo-2 ligand, and recovering the Apo-2 ligand from the host cells or the cell culture media. Optionally, one or more divalent metal ions are used during the recovery or purification process.
In another embodiment, the invention provides a formulation comprising Apo-2 ligand and one or more divalent metal ions. The composition may be a pharmaceutically acceptable formulation useful, for instance, in inducing or stimulating apoptosis in mammalian cancer cells.
A further embodiment of the invention provides articles of manufacture and kits that include such Apo-2 ligand formulations containing one or more divalent metal ions. The articles of manufacture and kits include a container, a label on the container, and a formulation contained within the container. The label on the container indicates that the formulation can be used for certain therapeutic or non-therapeutic applications. The formulation contains Apo-2 ligand and one or more divalent ions.
In another embodiment, the invention provides Apo-2 ligand polypeptides made in accordance with the methods described herein. Such Apo-2 ligands may comprise amino acids 114-281 of FIG. 1 (SEQ ID NO:1), amino acids 1-281 of FIG. 1 (SEQ ID NO:1), as well as biologically active fragments or variants thereof.
In a still further embodiment, the invention provides Apo-2 ligand variants. Particularly, the invention provides Apo-2 ligand variants comprising one or more amino acid substitutions in the native sequence of Apo-2 ligand (FIG. 1; SEQ ID NO:1). Apo-2 ligand variants comprising alanine substitutions are provided in Table I below. The invention also provides nucleic acid molecules encoding such Apo-2L variants and vectors and host cells containing nucleic acid molecules encoding the Apo-2L variants.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the nucleotide sequence of human Apo-2 ligand cDNA (SEQ ID NO:2) and its derived amino acid sequence (SEQ ID NO:1). The “N” at nucleotide position 447 (in SEQ ID NO:2) is used to indicate the nucleotide base may be a “T” or “G”.
FIG. 2 provides the crystal structure of Apo-2L. FIG. 2A shows a view of the trimer along the three fold axis. Each monomer is identical. The ordered protein structure commences at residue 120, residues 131-141 are disordered, as are residues 195-201 (marked as dashed lines). The zinc binding site including the three symmetry related cysteines and the solvent ligand are shown as space filling diagrams. FIG. 2B provides cross-eyed stereo close up view of the zinc binding site; the angles between Sγ-zinc-Sγ are 112° and the Sγ-zinc-solvent angles are 107° with 2.3 Angstrom zinc-sulfur and 2.3 Angstrom zinc-solvent bond distances. FIGS. 2 (and 5) were made with the programs Molscript [Kraulis et al., J. Appl. Cryst., 24:946-950 (1991)] and Raster3D [Merrit et al., Acta Cryst., D50:869-873 (1994)]. FIG. 2C provides a summary of the crystallographic data from the experiment described in Example 2.
FIG. 3 shows a sequence alignment of selected TNF family members: Apo2L (SEQ ID NO:1); TNF-beta (SEQ ID NO:3); TNF-alpha (SEQ ID NO:4); CD40L (SEQ ID NO:5); FasL (SEQ ID NO:6); RANKL (SEQ ID NO:7). Arrows over the sequence indicate beta-strands in Apo2L. The numbering over the aligned sequences corresponds to the Apo2L sequence numbering provided in FIG. 1 (SEQ ID NO:1).
FIG. 4 provides bioassay data showing the functional importance of the zinc binding site. SK-MES-1 cell viability was determined by a fluorescence assay of metabolic activity after overnight incubation with various concentrations of Apo-2L (form 114-281), or Apo-2L (form 114-281) treated with chelating agents to remove the zinc.
FIG. 5 shows mutational analysis mapped onto a space-filling model of Apo-2L. The trimer is oriented as in FIG. 2. Residues with a greater than 5-fold decrease in bioactivity when mutated to alanine are labeled and darkly shaded. Other residues that have been mutated are shown in medium shading and a few of these residues are also labeled.
FIG. 6 shows circular dichroic spectra of Apo-2L (form 114-281) before and after treatment to remove the bound zinc.
FIG. 7 shows thermal denaturation of Apo-2L before and after zinc removal monitored by circular dichroism at 225 nm. The dynode voltage is reported for 2 micromolar solutions of Apo-2L.
FIG. 8 shows the effect (time course) of ZnSO4 additions on soluble Apo-2L product accumulation (gm/L) in an E. coli expression system using an AP promoter.
FIG. 9 shows the elution profiles from MPHS chromatography of cell lysates from the E. coli expression system (see Example 8) conducted in the presence or absence of ZnSO4.
FIG. 10 shows the effect (time course) of ZnSO4 addition on soluble Apo-2L product accumulation (gm/L) in an E. coli expression system using a trp promoter.
FIG. 11 shows the effect (time course) of CoCl2 addition on soluble Apo-2L product accumulation (gm/L) in an E. coli expression system using an AP promoter.
FIG. 12 shows the pAPApo2-P2RU plasmid construct.
FIG. 13 shows the pAPOK5 plasmid construct.



DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
I. Definitions
The terms “Apo-2 ligand”, “Apo-2L”, and “TRAIL” are used herein to refer to a polypeptide sequence which includes amino acid residues 114-281, inclusive, 95-281, inclusive, residues 92-281, inclusive, residues 91-281, inclusive, residues 41-281, inclusive, residues 15-281, inclusive, or residues 1-281, inclusive, of the amino acid sequence shown in FIG. 1 (SEQ ID NO:1), as well as biologically active fragments, deletional, insertional, or substitutional variants of the above sequences. In one embodiment, the polypeptide sequence comprises residues 114-281 of FIG. 1 (SEQ ID NO:1). Optionally, the polypeptide sequence comprises residues 92-281 or residues 91-281 of FIG. 1 (SEQ ID NO:1). The Apo-2L polypeptides may be encoded by the native nucleotide sequence shown in FIG. 1 (SEQ ID NO:2). Optionally, the codon which encodes residue Pro119 (FIG. 1; SEQ ID NO:2) may be “CCT” or “CCG”. In another preferred embodiment, the fragments or variants are biologically active and have at least about 80% amino acid sequence identity, more preferably at least about 90% sequence identity, and even more preferably, at least 95%, 96%, 97%, 98%, or 99% sequence identity with any one of the above sequences. The definition encompasses substitutional variants of Apo-2 ligand in which at least one of its native amino acids are substituted by an alanine residue. Preferred substitutional variants include one or more of the residue substitutions identified in Table I below. The definition also encompasses a native sequence Apo-2 ligand isolated from an Apo-2 ligand source or prepared by recombinant or synthetic methods. The Apo-2 ligand of the invention includes the polypeptides referred to as Apo-2 ligand or TRAIL disclosed in WO97/01633 published Jan. 16, 1997 and WO97/25428 published Jul. 17, 1997. The terms “Apo-2 ligand” or “Apo-2L” are used to refer generally to forms of the Apo-2 ligand which include monomer, dimer or trimer forms of the polypeptide. All numbering of amino acid residues referred to in the Apo-2L sequence use the numbering according to FIG. 1 (SEQ ID NO:1), unless specifically stated otherwise. For instance, “D203” or “Asp203” refers to the aspartic acid residue at position 203 in the sequence provided in FIG. 1 (SEQ ID NO:1).
The term “Apo-2 ligand extracellular domain” or “Apo-2 ligand ECD” refers to a form of Apo-2 ligand which is essentially free of transmembrane and cytoplasmic domains. Ordinarily, the ECD will have less than 1% of such transmembrane and cytoplasmic domains, and preferably, will have less than 0.5% of such domains.
The term “Apo-2 ligand monomer” or “Apo-2L monomer” refers to a covalent chain of an extracellular domain sequence of Apo-2L.
The term “Apo-2 ligand dimer” or “Apo-2L dimer” refers to two Apo-2L monomers joined in a covalent linkage via a disulfide bond. The term as used herein includes free standing Apo-2L dimers and Apo-2L dimers that are within trimeric forms of Apo-2L (i.e., associated with another Apo-2L monomer).
The term “Apo-2 ligand trimer” or “Apo-2L trimer” refers to three Apo-2L monomers that are non-covalently associated.
“TNF family member” is used in a broad sense to refer to various polypeptides that share some similarity to tumor necrosis factor (TNF) with respect to structure or function. Certain structural and functional characteristics associated with the TNF family of polypeptides are known in the art and described, for example, in the above Background of the Invention. Such polypeptides include but are not limited to those polypeptides referred to in the art as TNF-alpha, TNF-beta, CD40 ligand, CD30 ligand, CD27 ligand, OX-40 ligand, 4-1BB ligand, Apo-1 ligand (also referred to as Fas ligand or CD95 ligand), Apo-2 ligand (also referred to as TRAIL), Apo-3 ligand (also referred to as TWEAK), APRIL, OPG ligand (also referred to as RANK ligand, ODF, or TRANCE), and TALL-1 (also referred to as BlyS, BAFF or THANK) [See, e.g., Gruss and Dower, Blood, 85:3378-3404 (1995); Pitti et al., J. Biol. Chem., 271:12687-12690 (1996); Wiley et al., Immunity, 3:673-682 (1995); Browning et al., Cell, 72:847-856 (1993); Armitage et al. Nature, 357:80-82 (1992), WO 97/01633 published Jan. 16, 1997; WO 97/25428 published Jul. 17, 1997; Marsters et al., Curr. Biol., 8:525-528 (1998); Chicheportiche et al., Biol. Chem., 272:32401-32410 (1997); Hahne et al., J. Exp. Med., 188:1185-1190 (1998); WO98/28426 published Jul. 2, 1998; WO98/46751 published Oct. 22, 1998; WO/98/18921 published May 7, 1998; Moore et al., Science, 285:260-263 (1999); Shu et al., J. Leukocyte Biol., 65:680 (1999); Schneider et al., J. Exp. Med., 189:1747-1756 (1999); Mukhopadhyay et al., J. Biol. Chem., 274:15978-15981 (1999)].
The term “epitope tagged” when used herein refers to a chimeric polypeptide comprising Apo-2 ligand, or a portion thereof, fused to a “tag polypeptide”. The tag polypeptide has enough residues to provide an epitope against which an antibody can be made, yet is short enough such that it does not interfere with activity of the Apo-2 ligand. The tag polypeptide preferably also is fairly unique so that the antibody does not substantially cross-react with other epitopes. Suitable tag polypeptides generally have at least six amino acid residues and usually between about 8 to about 50 amino acid residues (preferably, between about 10 to about 20 residues).
The term “divalent metal ion” refers to a metal ion having two positive charges. Examples of divalent metal ions for use in the present invention include but are not limited to zinc, cobalt, nickel, cadmium, magnesium, and manganese. Particular forms of such metals that may be employed include salt forms (e.g., pharmaceutically acceptable salt forms), such as chloride, acetate, carbonate, citrate and sulfate forms of the above mentioned divalent metal ions. A preferred divalent metal ion for use in the present invention is zinc, and more preferably, the salt form, zinc sulfate. Divalent metal ions, as described herein, are preferably employed in concentrations or amounts (e.g., effective amounts) which are sufficient to, for example, (1) enhance storage stability of Apo-2L trimers over a desired period of time, (2) enhance production or yield of Apo-2L trimers in a recombinant cell culture or purification method, (3) enhance solubility (or reduce aggregation) of Apo-2L trimers, or (4) enhance Apo-2L trimer formation.
“Isolated,” when used to describe the various proteins disclosed herein, means protein that has been identified and separated and/or recovered from a component of its natural environment. Contaminant components of its natural environment are materials that would typically interfere with diagnostic or therapeutic uses for the protein, and may include enzymes, hormones, and other proteinaceous or non-proteinaceous solutes. In preferred embodiments, the protein will be purified (1) to a degree sufficient to obtain at least 15 residues of N-terminal or internal amino acid sequence by use of a spinning cup sequenator, or (2) to homogeneity by SDS-PAGE under non-reducing or reducing conditions using Coomassie blue or, preferably, silver stain. Isolated protein includes protein in situ within recombinant cells, since at least one component of the Apo-2 ligand natural environment will not be present. Ordinarily, however, isolated protein will be prepared by at least one purification step.
An “isolated” Apo-2 ligand nucleic acid molecule is a nucleic acid molecule that is identified and separated from at least one contaminant nucleic acid molecule with which it is ordinarily associated in the natural source of the Apo-2 ligand nucleic acid. An isolated Apo-2 ligand nucleic acid molecule is other than in the form or setting in which it is found in nature. Isolated Apo-2 ligand nucleic acid molecules therefore are distinguished from the Apo-2 ligand nucleic acid molecule as it exists in natural cells. However, an isolated Apo-2 ligand nucleic acid molecule includes Apo-2 ligand nucleic acid molecules contained in cells that ordinarily express Apo-2 ligand where, for example, the nucleic acid molecule is in a chromosomal location different from that of natural cells.
“Percent (%) amino acid sequence identity” with respect to the sequences identified herein is defined as the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the Apo-2 ligand sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art can determine appropriate parameters for measuring alignment, including assigning algorithms needed to achieve maximal alignment over the full-length sequences being compared. For purposes herein, percent amino acid identity values can be obtained using the sequence comparison computer program, ALIGN-2, which was authored by Genentech, Inc. and the source code of which has been filed with user documentation in the US Copyright Office, Washington, D.C., 20559, registered under the US Copyright Registration No. TXU510087. The ALIGN-2 program is publicly available through Genentech, Inc., South San Francisco, Calif. All sequence comparison parameters are set by the ALIGN-2 program and do not vary.
The term “control sequences” refers to DNA sequences necessary for the expression of an operably linked coding sequence in a particular host organism. The control sequences that are suitable for prokaryotes, for example, include a promoter, optionally an operator sequence, and a ribosome binding site. Eukaryotic cells are known to utilize promoters, polyadenylation signals, and enhancers.
Nucleic acid is “operably linked” when it is placed into a functional relationship with another nucleic acid sequence. For example, DNA for a presequence or secretory leader is operably linked to DNA for a polypeptide if it is expressed as a preprotein that participates in the secretion of the polypeptide; a promoter or enhancer is operably linked to a coding sequence if it affects the transcription of the sequence; or a ribosome binding site is operably linked to a coding sequence if it is positioned so as to facilitate translation. Generally, “operably linked” means that the DNA sequences being linked are contiguous, and, in the case of a secretory leader, contiguous and in reading phase. However, enhancers do not have to be contiguous. Linking is accomplished by ligation at convenient restriction sites. If such sites do not exist, the synthetic oligonucleotide adaptors or linkers are used in accordance with conventional practice.
“Biologically active” or “biological activity” for the purposes herein means (a) having the ability to induce or stimulate apoptosis in at least one type of mammalian cancer cell or virally-infected cell in vivo or ex vivo; (b) capable of raising an antibody, i.e., immunogenic; (c) capable of binding and/or stimulating a receptor for Apo-2L; or (d) retaining the activity of a native or naturally-occurring Apo-2L polypeptide.
The terms “apoptosis” and “apoptotic activity” are used in a broad sense and refer to the orderly or controlled form of cell death in mammals that is typically accompanied by one or more characteristic cell changes, including condensation of cytoplasm, loss of plasma membrane microvilli, segmentation of the nucleus, degradation of chromosomal DNA or loss of mitochondrial function. This activity can be determined and measured, for instance, by cell viability assays, FACS analysis or DNA electrophoresis.
The terms “cancer”, “cancerous”, or “malignant” refer to or describe the physiological condition in mammals that is typically characterized by unregulated cell growth. Examples of cancer include but are not limited to, carcinoma, lymphoma, leukemia, blastoma, and sarcoma. More particular examples of such cancers include squamous cell carcinoma, small-cell lung cancer, non-small cell lung cancer, glioma, gastrointestinal cancer, renal cancer, ovarian cancer, liver cancer, colorectal cancer, endometrial cancer, kidney cancer, prostate cancer, thyroid cancer, neuroblastoma, pancreatic cancer, glioblastoma multiforme, cervical cancer, stomach cancer, bladder cancer, hepatoma, breast cancer, colon carcinoma, and head and neck cancer.
The terms “treating”, “treatment” and “therapy” as used herein refer to curative therapy, prophylactic therapy, and preventative therapy.
The term “mammal” as used herein refers to any mammal classified as a mammal, including humans, cows, horses, dogs and cats. In a preferred embodiment of the invention, the mammal is a human.
II. Compositions and Methods of the Invention
A novel cytokine related to the TNF ligand family, the cytokine identified herein as “Apo-2 ligand” has been described. The predicted mature amino acid sequence of human Apo-2 ligand contains 281 amino acids, and has a calculated molecular weight of approximately 32.5 kDa. The absence of a signal sequence and the presence of an internal hydrophobic region suggests that Apo-2 ligand is a type II transmembrane protein. Soluble extracellular domain Apo-2 ligand polypeptides have also been described. See, e.g., WO97/25428 published Jul. 17, 1997. Apo-2L substitutional variants have further been described. Alanine scanning techniques have been utilized to identify various substitutional variant molecules having biological activity.
Particular substitutional variants of the Apo-2 ligand include those in which at least one amino acid is substituted by an alanine residue. These substitutional variants are identified, for example, as “D203A”; “D218A” and “D269A.” This nomenclature is used to identify Apo-2 ligand variants wherein the aspartic acid residues at positions 203, 218, and/or 269 (using the numbering shown in FIG. 1 SEQ ID NO:1)) are substituted by alanine residues. Optionally, the Apo-2L variants may comprise one or more of the alanine substitutions which are recited in Table I below.
The x-ray crystal structure of the extracellular domain of Apo-2 ligand is now provided in the present invention, and alanine-scanning mutagenesis has been performed to provide the mapping of its receptor contact regions. The structure obtained for Apo-2 ligand reveals a homotrimeric protein which contains a novel divalent metal ion (zinc) binding site that coordinates the interaction of the Apo-2 ligand trimer molecule's three subunits.
The x-ray structure of Apo-2L was determined by molecular replacement using a model of TNF-alpha [Eck et al., J. Biol. Chem., 264:17595-17605 (1989)] and refined to 3.9 Angstrom (for the 114-281 residue form) and 1.3 Angstrom (for the D218A variant; 91-281 form). Like other members of the TNF family, Apo-2L appears to comprise a compact trimer formed of three jelly roll monomers which bury approximately 5100 Angstrom2 (1700 Angstrom2 per monomer) to form the globular trimer (See FIG. 2A). The position of the core beta-strands was well conserved compared to the other structurally characterized members of the TNF family, TNF-alpha [Eck et al., supra; Jones et al., Nature, 338:225-228 (1989)], TNF-beta [Eck et al., J. Biol. Chem., 267:2119-2122 (1992)], and CD40L [Karpusas et al., Structure, 3:1031-1039 (1995)], with a r.m.s.d. of 0.8 Angstrom when compared to the core strands of TNF-alpha or TNF-beta. None of the residues in the Apo-2L trimer interface appear to be absolutely conserved across the sequences of the all the presently known human TNF family members; however, the hydrophobic chemical nature of these residues is preserved. (See FIG. 3). The conserved residues in the Apo-2L trimer interface cluster near the base (the widest part of the trimer) and along the three-fold axis. Near the top of the Apo-2L trimer interface in the vicinity of Cys230, the structures appear to diverge, and the conformation of the 190's and 230's loops are variable in each structure.
In contrast to the beta-scaffold core, the structure of the loops and receptor binding surfaces varies considerably among the TNF family members. One difference between the structure of Apo-2 ligand and the structures of TNF-alpha, TNF-beta, and CD40L is the connections between strands A and A′. In TNF-alpha, TNF-beta, and CD40L, strand A is followed by a compact loop. In Apo-2 ligand, a 15-residue insertion lengthens this loop and alters its conformation. The first part of the loop (residues 131 to 141) is disordered while the second part of the loop (residues 142 to 154) crosses the surface of the molecule from one monomer-monomer interface to the next (see FIG. 2A) with a conformation that resembles CD40L in its C-terminal portion.
Applicants surprisingly found a novel divalent metal ion (zinc) binding site buried near the top of the trimerization interface. The TNF family members can be divided by sequence analysis into three groups with respect to Cys230: (1) proteins such as TNF-alpha and Fas ligand in which a cysteine residue at the position corresponding to Cys230 is accompanied by another cysteine in the adjacent loop (the 194-203 loop in Apo-2L) with which it can form a disulfide bridge precluding it from interacting with a metal ion, (2) proteins without a cysteine corresponding to Cys230 (such as TNF-beta and OPGL), and (3) proteins which have only one cysteine residue corresponding to Cys230. Apo-2L and its orthologs in other species meet the latter criteria (i.e., proteins which have only Cys230) and are expected to bind divalent metal ions at the trimer surface. The conformation of the main chain immediately prior to Cys230 in Apo-2L differs from the disulfide containing TNF family members such as TNF-alpha and CD40L. In Apo-2L, the side chain of Cys230 is oriented towards the interface instead of away from it.
The Cys230 residue in each Apo-2L monomer point inward toward the trimer axis and coordinate a divalent metal ion in conjunction with an interior solvent molecule. This divalent metal ion binding site exhibits slightly distorted tetrahedral geometry with bonds and angles appropriate for a zinc binding site and is completely inaccessible to solvent (see FIG. 2B). The identity of the bound metal was confirmed using inductively coupled plasma atomic emission spectrometry (ICP-AES) (see Example 5). In a quantitative analysis for Cd, Co, Zn, Ni, and Cu using ICP-AES, 0.79 moles of Zn and 0.06 moles of Co per molecule of Apo-2L trimer were detected demonstrating that the bound ion in the structure was zinc at approximately a one to one molar ratio (see Example 5). The importance of this site was demonstrated by the observation that alanine substitution of Cys230 resulted in a >8-fold decreased apoptotic activity (See Example 7). Furthermore, removal of the bound metal from Apo-2L by dialysis against chelating agents resulted in a 7-fold decrease in DR5 affinity and a >90-fold decrease in apoptotic activity (see Example 6). Upon removal of the Zn, the cysteines became prone to oxidation and disulfide-linked Apo-2L dimers were formed which had decreased apoptotic activity. Since the metal binding site appears to be buried in the Apo-2L trimer structure and is not expected to contact receptor, the data suggests that divalent metal ion binding may be important to maintain the trimer structure and stability of Apo-2L.
In order to map Apo-2 ligand's receptor binding site, amino acid residues important for receptor binding and biological activity were identified by alanine-scanning mutagenesis. [Cunningham et al., Science, 244:1081-1085 (1989)]. Single alanine substitutions at residues Arg149, Gln205, Val207, Tyr216, Glu236, or Tyr237 resulted in a greater than 5-fold decrease in apoptotic activity in a bioassay and showed decreased affinity for the receptors (See Examples 3 and 4). Apo-2L binding to DR4, DR5 and DcR2 was most affected by alanine substitutions at residues Gln205, Tyr216, Glu236, or Tyr237, which resulted in at least a 5-fold decreased affinity against all three receptors. All of these variants with reduced apoptotic activity also exhibited impaired binding to either DR4 or DR5 (or both) suggesting that receptor binding is required for apoptotic activity.
Alanine substitutions at residues Asp218 and Asp269 resulted in Apo-2L variants having increased apoptotic activity. (See Example 4). Residue Asp218 is located near Tyr216, which is one of the required residues for apoptotic activity. A comparison to the low resolution Apo-2L structure (114-281 form) suggests that the conformation of the 216-220 loop does not appear to be significantly altered by the presence of the D218A mutation.
When the results of the mutagenesis analysis were mapped to the Apo-2L trimer structure, the functional epitope on Apo-2L for receptor binding and biological activity was found to be located on the surface formed by the junction of two monomers (see FIG. 5), similar to TNF-beta. A shallow groove at the monomer-monomer interface forms the receptor binding site with both monomers contributing to the binding site. Residues Arg32, Tyr87, and Asp143 in TNF-alpha (corresponding to Apo-2L residues Arg158, Tyr216, and Asp267) also make contributions to TNF receptor binding. [Goh et al., Protein Engineering, 4:785-791 (1991)]. In contrast, residues of TNF-alpha (corresponding to residues Gln205, Glu236, and Tyr237 of Apo-2L) play only a minor role in TNFR binding. Thus, while for TNF-alpha the base of the trimer structure makes the most important contribution to receptor binding, in Apo-2L, important receptor binding residues are also presented on the top of the trimer structure. Apo-2L appears to be unique among the TNF family members of known structure in having a larger and more extended contact surface for interaction with its target receptors. It is believed that preferred Apo-2L variants will comprise native residues (i.e., will not be mutated) at positions corresponding to Arg149, Gln205, Val207, Tyr216, Glu236, and/or Tyr237.
The description below relates to methods of producing Apo-2 ligand by culturing host cells transformed or transfected with a vector containing Apo-2 ligand encoding nucleic acid and recovering the polypeptide from the cell culture.
The DNA encoding Apo-2 ligand may be obtained from any cDNA library prepared from tissue believed to possess the Apo-2 ligand mRNA and to express it at a detectable level. Accordingly, human Apo-2 ligand DNA can be conveniently obtained from a cDNA library prepared from human tissues, such as the bacteriophage library of human placental cDNA as described in WO97/25428. The Apo-2 ligand-encoding gene may also be obtained from a genomic library or by oligonucleotide synthesis.
Libraries can be screened with probes (such as antibodies to the Apo-2 ligand or oligonucleotides of at least about 20-80 bases) designed to identify the gene of interest or the protein encoded by it. Screening the cDNA or genomic library with the selected probe may be conducted using standard procedures, such as described in Sambrook et al., Molecular Cloning: A Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989). An alternative means to isolate the gene encoding Apo-2 ligand is to use PCR methodology [Sambrook et al., supra; Dieffenbach et al., PCR Primer: A Laboratory Manual (Cold Spring Harbor Laboratory Press, 1995)].
Amino acid sequence fragments or variants of Apo-2 ligand can be prepared by introducing appropriate nucleotide changes into the Apo-2 ligand DNA, or by synthesis of the desired Apo-2 ligand polypeptide. Such fragments or variants represent insertions, substitutions, and/or deletions of residues within or at one or both of the ends of the intracellular region, the transmembrane region, or the extracellular region, or of the amino acid sequence shown for the full-length Apo-2 ligand in FIG. 1 (SEQ ID NO:1). Any combination of insertion, substitution, and/or deletion can be made to arrive at the final construct, provided that the final construct possesses, for instance, a desired biological activity or apoptotic activity as defined herein. In a preferred embodiment, the fragments or variants have at least about 80% amino acid sequence identity, more preferably, at least about 90% sequence identity, and even more preferably, at least 95%, 96%, 97%, 98% or 99% sequence identity with the sequences identified herein for the intracellular, transmembrane, or extracellular domains of Apo-2 ligand, or the full-length sequence for Apo-2 ligand. The amino acid changes also may alter post-translational processes of the Apo-2 ligand, such as changing the number or position of glycosylation sites or altering the membrane anchoring characteristics.
Variations in the Apo-2 ligand sequence as described above can be made using any of the techniques and guidelines for conservative and non-conservative mutations set forth in U.S. Pat. No. 5,364,934. These include oligonucleotide-mediated (site-directed) mutagenesis, alanine scanning, and PCR mutagenesis.
Scanning amino acid analysis can be employed to identify one or more amino acids along a contiguous sequence. Among the preferred scanning amino acids are relatively small, neutral amino acids. Such amino acids include alanine, glycine, serine and cysteine. Alanine is typically a preferred scanning amino acid among this group because it eliminates the side-chain beyond the beta-carbon and is less likely to alter the main-chain conformation of the variant. [Cunningham et al., Science, 244:1081 (1989)]. Alanine is also typically preferred because it is the most common amino acid. Further, it is frequently found in both buried and exposed positions [Creighton, The Proteins, (W.H. Freeman & Co., NY); Chothia, J. Mol. Biol., 150:1 (1976)].
Particular Apo-2L variants of the present invention include those Apo-2L polypeptides which include one or more of the recited alanine substitutions provided in TABLE I below. Such Apo-2L variants will typically comprise a non-naturally occurring amino acid sequence which differs from a native Apo-2L amino acid sequence (such as provided in FIG. 1; SEQ ID NO:1, for a full length or mature form of Apo-2L or an extracellular domain sequence thereof) in at least one or more amino acids. Optionally, the one or more amino acids which differ in the Apo-2L variant as compared to a native Apo-2L will comprise amino acid substitution(s) such as those indicated in Table I. Apo-2L variants of the invention include soluble Apo-2L variants comprising residues 91-281, 92-281, 95-281 or 114-281 of FIG. 1 (SEQ ID NO:1) and having one or more amino acid substitutions recited in TABLE I. Preferred Apo-2L variants will include those variants comprising residues 91-281, 92-281, 95-281 or 114-281 of FIG. 1 (SEQ ID NO:1) and having one or more amino acid substitutions recited in TABLE I which enhance biological activity, such as receptor binding.
Variations in the Apo-2 ligand sequence also included within the scope of the invention relate to amino-terminal derivatives or modified forms. Such Apo-2 ligand sequences include any of the Apo-2 ligand polypeptides described herein having a methionine or modified methionine (such as formyl methionyl or other blocked methionyl species) at the N-terminus of the polypeptide sequence.
The nucleic acid (e.g., cDNA or genomic DNA) encoding native or variant Apo-2 ligand may be inserted into a replicable vector for further cloning (amplification of the DNA) or for expression. Various vectors are publicly available. The vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence, each of which is described below. Optional signal sequences, origins of replication, marker genes, enhancer elements and transcription terminator sequences that may be employed are known in the art and described in further detail in WO97/25428.
Expression and cloning vectors usually contain a promoter that is recognized by the host organism and is operably linked to the Apo-2 ligand nucleic acid sequence. Promoters are untranslated sequences located upstream (5′) to the start codon of a structural gene (generally within about 100 to 1000 bp) that control the transcription and translation of a particular nucleic acid sequence, such as the Apo-2 ligand nucleic acid sequence, to which they are operably linked. Such promoters typically fall into two classes, inducible and constitutive. Inducible promoters are promoters that initiate increased levels of transcription from DNA under their control in response to some change in culture conditions, e.g., the presence or absence of a nutrient or a change in temperature. At this time a large number of promoters recognized by a variety of potential host cells are well known. These promoters are operably linked to Apo-2 ligand encoding DNA by removing the promoter from the source DNA by restriction enzyme digestion and inserting the isolated promoter sequence into the vector. Both the native Apo-2 ligand promoter sequence and many heterologous promoters may be used to direct amplification and/or expression of the Apo-2 ligand DNA.
Promoters suitable for use with prokaryotic and eukaryotic hosts are known in the art, and are described in further detail in WO97/25428.
A preferred method for the production of soluble Apo-2L in E. coli employs an inducible promoter for the regulation of product expression. The use of a controllable, inducible promoter allows for culture growth to the desirable cell density before induction of product expression and accumulation of significant amounts of product which may not be well tolerated by the host.
Three inducible promoter systems (T7 polymerase, trp and alkaline phosphatase (AP)) have been evaluated by Applicants for the expression of Apo-2L (form 114-281). The use of each of these three promoters resulted in significant amounts of soluble, biologically active Apo-2L trimer being recovered from the harvested cell paste. The AP promoter is preferred among these three inducible promoter systems tested because of tighter promoter control and the higher cell density and titers reached in harvested cell paste.
Construction of suitable vectors containing one or more of the above-listed components employs standard ligation techniques. Isolated plasmids or DNA fragments are cleaved, tailored, and re-ligated in the form desired to generate the plasmids required.
For analysis to confirm correct sequences in plasmids constructed, the ligation mixtures can be used to transform E. coli K12 strain 294 (ATCC 31,446) and successful transformants selected by ampicillin or tetracycline resistance where appropriate. Plasmids from the transformants are prepared, analyzed by restriction endonuclease digestion, and/or sequenced using standard techniques known in the art. [See, e.g., Messing et al., Nucleic Acids Res., 9:309 (1981); Maxam et al., Methods in Enzymology, 65:499 (1980)].
Expression vectors that provide for the transient expression in mammalian cells of DNA encoding Apo-2 ligand may be employed. In general, transient expression involves the use of an expression vector that is able to replicate efficiently in a host cell, such that the host cell accumulates many copies of the expression vector and, in turn, synthesizes high levels of a desired polypeptide encoded by the expression vector [Sambrook et al., supra]. Transient expression systems, comprising a suitable expression vector and a host cell, allow for the convenient positive identification of polypeptides encoded by cloned DNAs, as well as for the rapid screening of such polypeptides for desired biological or physiological properties. Thus, transient expression systems are particularly useful in the invention for purposes of identifying analogs and variants of Apo-2 ligand that are biologically active Apo-2 ligand.
Other methods, vectors, and host cells suitable for adaptation to the synthesis of Apo-2 ligand in recombinant vertebrate cell culture are described in Gething et al., Nature, 293:620-625 (1981); Mantei et al., Nature, 281:40-46 (1979); EP 117,060; and EP 117,058.
Suitable host cells for cloning or expressing the DNA in the vectors herein include prokaryote, yeast, or higher eukaryote cells. Suitable prokaryotes for this purpose include but are not limited to eubacteria, such as Gram-negative or Gram-positive organisms, for example, Enterobacteriaceae such as Escherichia, e.g., E. coli, Enterobacter, Erwinia, Klebsiella, Proteus, Salmonella, e.g., Salmonella typhimurium, Serratia, e.g., Serratia marcescans, and Shigella, as well as Bacilli such as B. subtilis and B. lichenifonnis (e.g., B. lichenifonnis 41P disclosed in DD 266,710 published 12 Apr. 1989), Pseudomonas such as P. aeruginosa, and Streptomyces. Preferably, the host cell should secrete minimal amounts of proteolytic enzymes.
E. coli is the preferred host cell for use in the present invention. E. coli is particularly well suited for the expression of Apo-2 ligand (form 114-281), a polypeptide of under 20 kd in size with no glycosylation requirement. As a production host, E. coli can be cultured to relatively high cell density and is capable of producing relatively high levels of heterologous proteins.
In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for Apo-2 ligand-encoding vectors. Suitable host cells for the expression of glycosylated Apo-2 ligand are derived from multicellular organisms. Examples of all such host cells, including CHO cells, are described further in WO97/25428.
Host cells are transfected and preferably transformed with the above-described expression or cloning vectors for Apo-2 ligand production and cultured in nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.
Transfection refers to the taking up of an expression vector by a host cell whether or not any coding sequences are in fact expressed. Numerous methods of transfection are known to the ordinarily skilled artisan, for example, CaPO4 and electroporation. Successful transfection is generally recognized when any indication of the operation of this vector occurs within the host cell.
Transformation means introducing DNA into an organism so that the DNA is replicable, either as an extrachromosomal element or by chromosomal integrant. Depending on the host cell used, transformation is done using standard techniques appropriate to such cells. The calcium treatment employing calcium chloride, as described in Sambrook et al., supra, or electroporation is generally used for prokaryotes or other cells that contain substantial cell-wall barriers. Infection with Agrobacterium tumefaciens is used for transformation of certain plant cells, as described by Shaw et al., Gene, 23:315 (1983) and WO 89/05859 published 29 Jun. 1989. In addition, plants may be transfected using ultrasound treatment as described in WO 91/00358 published 10 Jan. 1991.
For mammalian cells without such cell walls, the calcium phosphate precipitation method of Graham and van der Eb, Virology, 52:456-457 (1978) may be employed. General aspects of mammalian cell host system transformations have been described in U.S. Pat. No. 4,399,216. Transformations into yeast are typically carried out according to the method of Van Solingen et al., J. Bact., 130:946 (1977) and Hsiao et al., Proc. Natl. Acad. Sci. (USA), 76:3829 (1979). However, other methods for introducing DNA into cells, such as by nuclear microinjection, electroporation, bacterial protoplast fusion with intact cells, or polycations, e.g., polybrene, polyornithine, may also be used. For various techniques for transforming mammalian cells, see Keown et al., Methods in Enzymology, 185:527-537 (1990) and Mansour et al., Nature, 336:348-352 (1988).
Prokaryotic cells used to produce Apo-2 ligand may be cultured in suitable culture media as described generally in Sambrook et al., supra. Particular forms of culture media that 25, may be employed for culturing E. coli are described further in the Examples below. Mammalian host cells used to produce Apo-2 ligand may be cultured in a variety of culture media.
Examples of commercially available culture media include Ham's F10 (Sigma), Minimal Essential Medium (“MEM”, Sigma), RPMI-1640 (Sigma), and Dulbedco's Modified Eagle's Medium (“DMEM”, Sigma). Any such media may be supplemented as necessary with hormones and/or other growth factors (such as insulin, transferrin, or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such as HEPES), nucleosides (such as adenosine and thymidine), antibiotics (such as GentamycinTM drug), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range), and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to those skilled in the art. The culture conditions, such as temperature, pH, and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan.
In general, principles, protocols, and practical techniques for maximizing the productivity of mammalian cell cultures can be found in Mammalian Cell Biotechnology: A Practical Approach, M. Butler, ed. (IRL Press, 1991).
In accordance with the present invention, one or more divalent metal ions will typically be added to or included in the culture media for culturing or fermenting the host cells. The divalent metal ions are preferably present in or added to the culture media at a concentration level sufficient to enhance storage stability, enhance solubility, or assist in forming stable Apo-2L trimers coordinated by one or more zinc ions. The amount of divalent metal ions which may be added will be dependent, in part, on the host cell density in the culture or potential host cell sensitivity to such divalent metal ions. At higher host cell densities in the culture, it may be beneficial to increase the concentration of divalent metal ions. If the divalent metal ions are added during or after product expression by the host cells, it may be desirable to adjust or increase the divalent metal ion concentration as product expression by the host cells increases. It is generally believed that trace levels of divalent metal ions which may be present in typical commonly available cell culture media may not be sufficient for stable trimer formation. Thus, addition of further quantities of divalent metal ions, as described herein, is preferred.
The divalent metal ions are preferably added to the culture media at a concentration which does not adversely or negatively affect host cell growth, if the divalent metal ions are being added during the growth phase of the host cells in the culture. In shake flask cultures, it was observed that ZnSO4 added at concentrations of greater than 1 mM can result in lower host cell density. Those skilled in the art appreciate that bacterial cells can sequester metal ions effectively by forming metal ion complexes with cellular matrices. Thus, in the cell cultures, it is preferable to add the selected divalent metal ions to the culture media after the growth phase (after the desired host cell density is achieved) or just prior to product expression by the host cells. To ensure that sufficient amounts of divalent metal ions are present, additional divalent metal ions may be added or fed to the cell culture media during the product expression phase.
The divalent metal ion concentration in the culture media should not exceed the concentration which may be detrimental or toxic to the host cells. In the methods of the invention employing the host cell, E. coli, it is preferred that the concentration of the divalent metal ion concentration in the culture media does not exceed about 1 mM (preferably, ≦1 mM). Even more preferably, the divalent metal ion concentration in the culture media is about 50 micromolar to about 250 micromolar. Most preferably, the divalent metal ion used in such methods is zinc sulfate. It is desirable to add the divalent metal ions to the cell culture in an amount wherein the metal ions and Apo-2 ligand trimer can be present at a one to one molar ratio.
The divalent metal ions can be added to the cell culture in any acceptable form. For instance, a solution of the metal ion can be made using water, and the divalent metal ion solution can then be added or fed to the culture media.
In one embodiment of the invention, the selected Apo-2L (form 114-281) is expressed in E. coli, and during the culturing or fermentation of the cell culture, the process parameters are set such that cellular activities are conducted at oxygen uptake rates of approximately 1.0 to 3.0 mmoles/L-min for cultures at approximately 40-50 gm/L dry cell weight. It is preferred that the newly synthesized nascent Apo-2L polypeptides have sufficient time for proper protein folding and trimerization of Apo-2L monomers. The growth phase of the fermentation process is preferably conducted at 30° C. Just prior to the commencement of product expression; the process temperature control set-point may remain at 30° C. or be down-shifted to 25° C. for the rest of the fermentation. Optionally, it may be desired to increase cell density in the cell culture, and the above-mentioned parameters may be adjusted (or increased) accordingly. For instance, it may be advantageous to increase cell density in the cell culture to increase volumetric yield. One skilled in the art can, by using routine techniques known in the art, incrementally increase the cell density and incrementally increase the above-mentioned parameters, if desired.
Expression of the Apo-2L may be measured in a sample directly, for example, by conventional Southern blotting, Northern blotting to quantitate the transcription of mRNA [Thomas, Proc. Natl. Acad. Sci. USA, 77:5201-5205 (1980)], dot blotting (DNA analysis), or in situ hybridization, using an appropriately labeled probe, based on the sequences provided herein. Various labels may be employed, most commonly radioisotopes, and particularly 32P. However, other techniques may also be employed, such as using biotin-modified nucleotides for introduction into a polynucleotide. The biotin then serves as the site for binding to avidin or antibodies, which may be labeled with a wide variety of labels, such as radionucleotides, fluorescers or enzymes. Alternatively, antibodies may be employed that can recognize specific duplexes, including DNA duplexes, RNA duplexes, and DNA-RNA hybrid duplexes or DNA-protein duplexes. The antibodies in turn may be labeled and the assay may be carried out where the duplex is bound to a surface, so that upon the formation of duplex on the surface, the presence of antibody bound to the duplex can be detected. Gene expression, alternatively, may be measured by immunological methods, such as immunohistochemical staining of cells or tissue sections and assay of cell culture or body fluids, to quantitate directly the expression of gene product. With immunohistochemical staining techniques, a cell sample is prepared, typically by dehydration and fixation, followed by reaction with labeled antibodies specific for the gene product coupled, where the labels are usually visually detectable, such as enzymatic labels, fluorescent labels, luminescent labels, and the like.
Antibodies useful for immunohistochemical staining and/or assay of sample fluids may be either monoclonal or polyclonal, and may be prepared in any mammal. Conveniently, the antibodies may be prepared against a native Apo-2 ligand polypeptide or against a synthetic peptide based on the DNA sequences provided herein or against exogenous sequence fused to Apo-2 ligand DNA and encoding a specific antibody epitope.
Apo-2 ligand preferably is recovered from the culture medium as a secreted polypeptide, although it also may be recovered from host cell lysates when directly produced without a secretory signal. If the Apo-2 ligand is membrane-bound, it can be released from the membrane using a suitable detergent solution (e.g. Triton-X 100) or its extracellular region may be released by enzymatic cleavage.
When Apo-2 ligand is produced in a recombinant cell other than one of human origin, the Apo-2 ligand is free of proteins or polypeptides of human origin. However, it is usually necessary to recover or purify Apo-2 ligand from recombinant cell proteins or polypeptides to obtain preparations that are substantially homogeneous as to Apo-2 ligand. As a first step, the culture medium or lysate may be centrifuged to remove particulate cell debris. Apo-2 ligand thereafter is purified from contaminant soluble proteins and polypeptides, with the following procedures being exemplary of suitable purification procedures: by fractionation on an ion-exchange column; ethanol precipitation; reverse phase HPLC; chromatography on silica or on a cation-exchange resin such as DEAE or CM; chromatofocusing; SDS-PAGE; ammonium sulfate precipitation; gel filtration using, for example, Sephadex G-75; diafiltration and protein A Sepharose columns to remove contaminants such as IgG.
In a preferred embodiment, the Apo-2 ligand can be isolated by affinity chromatography. Apo-2 ligand fragments or variants in which residues have been deleted, inserted, or substituted are recovered in the same fashion as native Apo-2 ligand, taking account of any substantial changes in properties occasioned by the variation. For example, preparation of an Apo-2 ligand fusion with another protein or polypeptide, e.g., a bacterial or viral antigen, facilitates purification; an immunoaffinity column containing antibody to the antigen can be used to adsorb the fusion polypeptide.
A protease inhibitor such as phenyl methyl sulfonyl fluoride (PMSF) also may be useful to inhibit proteolytic degradation during purification, and antibiotics may be included to prevent the growth of adventitious contaminants. One skilled in the art will appreciate that purification methods suitable for native Apo-2 ligand may require modification to account for changes in the character of Apo-2 ligand or its variants upon expression in recombinant cell culture.
During any such purification steps, it may be desirable to expose the recovered Apo-2L to a divalent metal ion-containing solution or to purification material (such as a chromatography medium or support) containing one or more divalent metal ions. In a preferred embodiment, the divalent metal ions and/or reducing agent is used during recovery or purification of the Apo-2L. Optionally, both divalent metal ions and reducing agent, such as DTT or BME, may be used during recovery or purification of the Apo-2L. It is believed that use of divalent metal ions during recovery or purification will provide for stability of Apo-2L trimer or preserve Apo-2L trimer formed during the cell culturing step.
A preferred method of recovering and purifying the expressed Apo-2L from prokaryotic host cells (most preferably from bacterial host cells) comprises the following steps: (a) extracting Apo-2L (intracellular) from E. coli cells; (b) stabilizing the properly folded Apo-2L in a buffer solution comprising divalent metal ions and/or reducing agent; (c) purifying the Apo-2L by chromatography using, sequentially, a cationic exchanger, a hydroxyapatite and a hydrophobic interaction chromatograph, and (d) selectively eluting Apo-2L in a buffer solution comprising divalent metal ions and/or reducing agent from each such chromatographic support. The divalent metal ions and the reducing agent utilized in such methods may include a Zn sulfate, Zn chloride, Co sulfate, DTT and BME, and more preferably, a Zn sulfate or DTT. An even more detailed description of this recovery and purification process is provided in Example 8 below.
As discussed above, such methods of the invention are applicable and useful for various other proteins, besides Apo-2L, which have improved activity when in a trimerized form or which require trimerization of the protein for activity.
Formulations comprising Apo-2 ligand and one or more divalent metal ions are also provided by the present invention. It is believed that such formulations will be particularly suitable for storage (and maintain Apo-2L trimerization), as well as for therapeutic administration. Preferred formulations will comprise Apo-2L and zinc or cobalt. More preferably, the formulation will comprise an Apo-2L and zinc or cobalt solution in which the metal is at a <2× molar ratio to the protein. If an aqueous suspension is desired, the divalent metal ion in the formulation may be at a >2× molar ratio to the protein. Using zinc sulfate, Applicants have found Apo-2L (form 114-281) precipitates and forms an aqueous suspension at about a 100 mM concentration of zinc sulfate in the formulation. Those skilled in the art will appreciate that at a >2× molar ratio, there may be an upper range of concentration of the divalent metal ion in the formulation at which the metal can become deleterious to the formulation or would be undesirable as a therapeutic formulation.
The formulations may be prepared by known techniques. For instance, the Apo-2L formulation may be prepared by buffer exchange on a gel filtration column.
Typically, an appropriate amount of a pharmaceutically-acceptable salt is used in the formulation to render the formulation isotonic. Examples of pharmaceutically-acceptable carriers include saline, Ringer's solution and dextrose solution. The pH of the formulation is preferably from about 6 to about 9, and more preferably from about 7 to about 7.5. Preferably, the pH is selected so as to ensure that the zinc remains bound to the Apo-2L. If the pH is too high or too low, the zinc does not remain bound to the Apo-2L and as a result, dimers of Apo-2L will tend to form. It will be apparent to those persons skilled in the art that certain carriers may be more preferable depending upon, for instance, the route of administration and concentrations of Apo-2 ligand and divalent metal ions.
Therapeutic compositions of the Apo-2L can be prepared by mixing the desired Apo-2L molecule having the appropriate degree of purity with optional pharmaceutically acceptable carriers, excipients, or stabilizers (Remington's Pharmaceutical Sciences, 16th edition, Oslo, A. ed. (1980)), in the form of lyophilized formulations, aqueous solutions or aqueous suspensions.
Acceptable carriers, excipients, or stabilizers are preferably nontoxic to recipients at the dosages and concentrations employed, and include buffers such as Tris, HEPES, PIPES, phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; and/or non-ionic surfactants such as TWEENTM, PLURONICSTM or polyethylene glycol (PEG).
Additional examples of such carriers include ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts, or electrolytes such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, and cellulose-based substances. Carriers for topical or gel-based forms include polysaccharides such as sodium carboxymethylcellulose or methylcellulose, polyvinylpyrrolidone, polyacrylate, polyoxyethylene-polyoxypropylene-block polymers, polyethylene glycol, and wood wax alcohols. For all administrations, conventional depot forms are suitably used. Such forms include, for example, microcapsules, nano-capsules, liposomes, plasters, inhalation forms, nose sprays, sublingual tablets, and sustained-release preparations.
Effective dosages of Apo-2 ligand in the formulations may be determined empirically, and making such determinations is within the skill in the art. It is presently believed that an effective dosage or amount of Apo-2 ligand may range from about 1 microgram/kg to about 100 mg/kg of body weight or more per day. Interspecies scaling of dosages can be performed in a manner known in the art, e.g., as disclosed in Mordenti et al., Pharmaceut. Res., 8:1351 (1991). Those skilled in the art will understand that the dosage of Apo-2 ligand that must be administered will vary depending on, for example, the mammal which will receive the Apo-2 ligand, the route of administration, and other drugs or therapies being administered to the mammal.
Apo-2L to be used for in vivo administration should be sterile. This is readily accomplished by filtration through sterile filtration membranes, prior to or following lyophilization and reconstitution. Apo-2L ordinarily will be stored in lyophilized form or in solution if administered systemically. If in lyophilized form, Apo-2L is typically formulated in combination with other ingredients for reconstitution with an appropriate diluent at the time for use. An example of a liquid formulation of Apo-2L is a sterile, clear, colorless unpreserved solution filled in a single-dose vial for subcutaneous injection.
Therapeutic Apo-2L formulations generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having a stopper pierceable by a hypodermic injection needle. The formulations are preferably administered as repeated intravenous (i.v.), subcutaneous (s.c.), intramuscular (i.m.) injections or infusions, or as aerosol formulations suitable for intranasal or intrapulmonary delivery (for intrapulmonary delivery see, e.g., EP 257,956).
Apo-2L can also be administered in the form of sustained-release preparations. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the protein, which matrices are in the form of shaped articles, e.g., films, or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (e.g., poly(2-hydroxyethyl-methacrylate) as described by Langer et al., J. Biomed. Mater. Res., 15: 167-277 (1981) and Langer, Chem. Tech., 12: 98-105 (1982) or poly(vinylalcohol)), polylactides (U.S. Pat. No. 3,773,919, EP 58,481), copolymers of L-glutamic acid and gamma ethyl-L-glutamate (Sidman et al., Biopolymers, 22: 547-556 (1983)), non-degradable ethylene-vinyl acetate (Langer et al., supra), degradable lactic acid-glycolic acid copolymers such as the Lupron Depot (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(−)-3-hydroxybutyric acid (EP 133,988).
The Apo-2L and its formulations described herein can be employed in a variety of therapeutic and non-therapeutic applications. Among these applications are methods of treating various cancers (provided above) and viral conditions. Such therapeutic and non-therapeutic applications are described, for instance, in WO97/25428 and WO97/01633.
An article of manufacture such as a kit containing Apo-2L useful for the diagnosis or treatment of the disorders described herein comprises at least a container and a label. Suitable containers include, for example, bottles, vials, syringes, and test tubes. The containers may be formed from a variety of materials such as glass or plastic. The container holds an Apo-2L formulation that is effective for diagnosing or treating the condition and may have a sterile access port (for example, the container may be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). The label on, or associated with, the container indicates that the formulation is used for diagnosing or treating the condition of choice. The article of manufacture may further comprise a second container comprising a pharmaceutically-acceptable buffer, such as phosphate-buffered saline, Ringer's solution, and dextrose solution. It may further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, needles, syringes, and package inserts with instructions for use. The article of manufacture may also comprise a second or third container with another active agent as described above.
The following examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way.
All patent and literature references cited in the present specification are hereby incorporated by reference in their entirety.
EXAMPLES
Commercially available reagents referred to in the examples were used according to manufacturer's instructions unless otherwise indicated. The source of those cells identified in the following examples, and throughout the specification, by ATCC accession numbers is the American Type Culture Collection, Manassas, Va.
Example 1
Expression and Purification of Apo-2L Variants
Alanine substitution variants of Apo-2L were constructed by oligonucleotide-directed mutagenesis (Kunkel et al., Proc. Natl. Acad. Sci., 82:488-492 (1985); Kunkel, Methods in Enzymology, 154:367-382 (1987)) of a plasmid (pAPOK5) (see FIG. 13), designed for the intracellular E. coli expression of the 91-281 amino acid form of Apo-2L under control of the trp promoter. pAPOK5 was constructed by using PCR to clone the Apo-2L cDNA (encoding residues 91-281) into plasmid pS1162 which carries the trp promoter. E. coli strain 294 transformed with the mutated plasmids were grown to mid-log phase at 37° C. in 250 mL M9 media plus 100 μM ZnSO4, expression was induced by addition of 25 μg/mL beta-indole acrylic acid, and the cultures were grown overnight at 30° C. Cells were harvested by centrifugation and frozen.
The cell pellet was homogenized in 6 volumes 0.1 M Tris-HCl pH 8, 0.2 M NaCl, 5 mM DTT, 1 mM EDTA, and Apo-2L was isolated from the soluble fraction by IMAC on a chelating hiTRAP column (Pharmacia) charged with nickel. The Apo-2L had a weak affinity for immobilized metal and could be eluted with low concentrations of imidazole. A final purification was obtained by cation exchange chromatography on a SP hiTRAP column (Pharmacia). Concentrations of purified Apo-2L variants were determined by absorbance measurements using an e280 of 1.4 mg−1 ml cm−1.
The Apo-2L variants identified by the oligonucleotide-directed mutagenesis are listed in table I.







TABLE I







Receptor binding and apoptotic activity of Apo2L variantsa


Ratio (variant/wild-type)












DR4-IgG
DR5-IgG
DcR2-IgG
Apoptosis


Variant
KD
KD
KD
ED50














Δzinc
6.3
6.6
11.2
90.0


R130A
3
2.7
1.3
1.9


N134A
1.0
0.8
1.0
1.5


L136A
3.3
1.5
1.4
0.8


S138A
2.1
1.3
2.2
1.2


N140A
1.4
1.9
0.9
1.1


S141A
2.3
1.3
2.4
1.3


K142A
2.6
1.9
2.7
2.0


N143A
2.1
2.0
1.3
1.5


R149A
1.8
2.2
1.6
3.5


S153A
2.3
1.2
2.1
0.9


E155A
1.6
2
1.4
2.5


R158A
2.4
1.3
6.5
1.4


S159A
4.7
2.2
3.4
0.9


R170A
1.1
2.2
0.6
0.9


K179A
0.9
0.9
1.1
2.0


R191A
7.8
3.9
3.2
2.2


Q193A
1.7
1.1
1.2
2.2


E195A
4.6
1.4
2.6
0.8


K197D
2
2.1
2.9
1.1


K201A
4.3
2.7
10
2.5


N202A
2.5
2.5
1.9
3.2


D203A
1.5
1.1
0.6
0.5


Q205A
13.1
6.3
10.8
690


V207A
2.2
2.8
2.1
5.6


Y213A
1.3
1
1.5
1.2


Y216A
14.5
8.9
9.0
320


D218A
1.3
1.9
1.1
0.3


C230S
4.1
7.1
6.7
8.0


E236A
6.0
9.8
8.4
10.8


Y237A
7.3
5.0
48
8.3


Y240A
1.8
0.8
1.8
1.1


K251A
1.9
2
2.4
0.8


S259A
4.3
2.0
1.4
3.3


H264A
1.9
2.0
1.4
3.1


D267A
5.7
1.9
5.5
1.11


D269A
1.7
0.5
0.9
0.2





aValues shown represent the ratio of variant to wild-type. For wild-type Apo-2L (residues 91-281), the Kd values for DR4-IgG, DR5-IgG and DcR2-IgG are 0.8 ± 0.3 nM, 0.9 ± 0.4 nM, and 0.3 ± 0.2 nM. Wild-type Apo-2L (residues 91-281) gave an ED50 of 24 ± 3.1 ng/mL in the apoptosis assay while the 114-281 form of Apo-2L was slightly more active and gave an ED50 of 16.0 ± 3.6 ng/ml. Only 2-fold changes from wild-type values are considered to be significant.






Example 2
Crystallography Analysis of Apo-2L
Crystals of Apo-2L (amino acid residues 114-281) were grown in 70 uL sitting drops containing 40 uL protein (at 2.6 mg/mL in 20 mM Tris, pH8.0), 20 uL 50 mM Tris pH 8.0, and 10 uL 8% peg 2K MME over a well solution of 50% peg 2K MME at 20° C. and were members of the spacegroup P63 with two monomer in the asymmetric unit and unit cell constants a=72.5, c=140 Angstrom and diffract to 3.9 Angstrom at room temperature. Crystals of D218A variant (see Example 1) grew in 14 uL sitting drops containing 4 uL of 4% MPD and 10 uL protein (1.7 mg/ml in 20 mM Tris pH 7.5) over a well solution of 32% MPD at 4° C. and were members of the spacegroup R32 with one monomer per asymmetric unit and unit cell parameters 66.4, c=197.7 Angstrom and diffracted to 1.3 Angstrom at −180° C. with synchroton radiation. Data sets diffracting to 3.9 Angstrom for the Apo-2L (residues 114-281) crystals and 1.9 Angstrom for the D218A variant were measured on a Rigaku rotating anode x-ray generator equipped with a MAR detector and processed with DENZO/SCALEPACK [Otwinowski et al., Proceedings of the CCP4 Study Weekend: Data Collection and Processing (eds. Sawyer et al.) pp. 56-62 Daresbury Laboratory, Daresbury, England, 1993]. A 1.3 Angstrom data set for the D218A variant was measured at the Advanced Photon Source at Argonne National Labs and was processed wtih HKL2000/SCALEPACK and had a Rsym of 6.4% (34% in the 1.35-1.30 shell), with 100% completeness and a redundancy of 12-fold, and I/<I>=12.4.
The native Apo-2L structure was solved by molecular placement using a model based on TNF-alpha (pdb code 1TNF) with the program Amore [Acta Cryst., D50:760-763 (1994)] and was refined [Brunger, X-PLOR: version 3.1, Yale Press, New Haven 1987] with strict 2-fold non-crystallographic restraints until a Rfree of 35%. This structure refined against the 1.9 Angstrom dataset until a Rfree of 25% and finally was refined against 1.3 Angstrom data with Refmac and SHELXL [Sheldrick et al., Methods in Enzymology, pp. 319-343, Academic Press, San Diego 1997] of Rfree=22% and Rfactor of 20% with good geometry (rmsd bonds 0.011 Angstrom, rmsd angle 1.7°). All residues fall within the allowed regions of a Ramachandran plot. During refinement, a 28 sigma peak of electron density was observed between symmetry related Cys230 on the trimer axis. This density was modeled as a zinc ion and refined with B-factor of 10. It is believed that a chlorine molecule on the trimer axis is present as the fourth ligand to the zinc. The final model consists of residues 120-130, 142-194, 203-281 with 170 solvent molecules and one zinc ion and one chloride ion. Residues 91-119, 131-141, and 195-202 are disordered. N-terminal sequencing of several crystals confirmed that the N-terminus is intact while mass spectrometry of the starting material shows that it is full length.
A summary of the crystallographic data is provided in FIG. 2C.
Example 3
Determination of Receptor Binding Affinity of Apo-2L Variants
Dissociation constants (Kd) for binding of Apo-2L variants (see Table I) to immobilized receptor immunoadhesins were determined from surface plasmon resonance (SPR) measurements on a Pharmacia BIAcore 1000.
DR5-IgG (also referred to as Apo-2-IgG) and DcR2-IgG receptor immunoadhesins were prepared as described in WO98/51793 published Nov. 19, 1998 and WO99/10484 published Mar. 9, 1999, respectively. DR4-IgG was prepared as follows. A mature DR4 ECD sequence (amino acids 1-218; Pan et al., supra) was cloned into a pCMV-1 Flag vector (Kodak) downstream of the Flag signal sequence and fused to the CH1, hinge and Fc region of human immunoglobulin G1 heavy chain as described previously [Aruffo et al., Cell, 61:1303-1313 (1990)]. The immunoadhesin was expressed by transient transfection into human 293 cells and purified from the cell supernatants by protein A affinity chromatography, as described by Ashkenazi et al., Proc. Natl. Acad. Sci., 88:10535-10539 (1991)].
The receptor immunoadhesin proteins were coupled to the sensor chip surface at a level of 1000-2000 resonance units using amine coupling chemistry (Pharmacia Biosensor). Sensorgrams were recorded for Apo-2L binding at concentrations ranging from 15.6 nM to 500 nM in 2-fold increments. The kinetics constants were determined by non-linear regression analysis and used to calculate the binding constants.
The results are shown in Table I.
Example 4
Apoptotic Activity of Apo-2L Variants In Vitro
A bioassay which measures cell viability from the metabolic conversion of a fluorescent dye was used to determine the apoptotic activity of Apo-2L variants. Serial 2-fold dilutions of Apo-2L (form 114-281) or Apo-2L variants (see Table I) were made in RPMI-1640 media (Gibco) containing 0.1% BSA, and 50 μL of each dilution was transferred to individual wells of 96-well Falcon tissue culture microplates. 50 μL of SK-MES-1 human lung carcinoma cells (ATCC HTB58) (in RMPI-1640, 0.1% BSA) were added at a density of 2×104 cells/well. These mixtures were incubated at 37° C. for 24 hours. At 20 hours, 25 μL of alamar Blue (AccuMed, Inc., Westlake, Ohio) was added. Cell number was determined by measuring the relative fluorescence at. 590 nm upon excitation at 530 nm. These data were analyzed by using a 4 parameter fit to calculate ED50, the concentration of Apo-2L giving a 50% reduction in cell viability.
Single alanine substitutions at residues Arg149, Gln205, Val207, Tyr216, Glu236 or Tyr237 resulted in a greater than 5-fold decrease in apoptotic activity in the bioassay and showed decreased affinity for DR4, DR5 and DcR2 (Table I). Apo-2L binding to these receptors was most affected by alanine substitution of Gln205, Tyr216, Glu236 and Tyr237, all of which resulted in at least a 5-fold decreased affinity for all three receptors. All of the Apo-2L variants with reduced apoptotic activity also exhibited impaired binding to either DR4 or DR5 (or both) suggesting that receptor-binding is required for the biological effect. Alanine substitution of Asp218 or Asp269 resulted in a greater than 2-fold increase in apoptotic activity. It is noteworthy that most alanine substitutions have similar effects on both DR4 and DR5 binding, the only exceptions being mutation of Gln193, Glu195, Ser259, His264, and Asp267, all of which had a greater than 5-fold effect on DR4 binding (decreasing affinity) but only a small or negligible effect on DR5 binding. Changes in DcR2-binding tended to parallel the effects observed for DR4-binding. Diminished apoptotic activity appears to be most closely linked with decreased DR5-binding suggesting that DR5 is required for death signaling in SK-MES in response to Apo-2L administration.
Example 5
Elemental and Quantitative Analysis to Determine Metal Content of Apo-2L
Elemental analysis of Apo-2L was performed by using inductively coupled plasma atomic emission spectrometry (ICP-AES). For this determination, a 2 mg/mL solution of Apo-2L (residues 114-281 produced in E. coli using methods disclosed in WO97/25428; additional quantities of divalent metal ions were not added during fermentation or purification in accordance with the methods described herein) formulated in 20 mM Tris pH 7.5 was used. Levels of Cd, Co, Zn, Ni, and Cu in this sample and in a portion of the formulation buffer were determined.












TABLE II





Sample
Cd
Co
Zn
Ni
Cu







Buffer
−0.058
−0.090
−0.098
−0.098
−0.082


Apo-2L
−0.058
  0.199
  1.712
−0.108
−0.075









The metals bound to the Apo-2L were Zn and Co (Table II). The calculated molar ratios were 0.79 moles of Zn per mole of Apo-2L trimer and 0.06 moles of Co per mole of Apo-2L trimer. These data indicate Apo-2L has one zinc binding site per trimer. The site is 85% occupied with metal in this preparation of Apo-2L.
Example 6
Effects of Removal of Bound Zinc from Apo-2L Using Chelating Agents
A sample of Apo-2L (form 114-281) was treated with chelating agents to remove the bound zinc. The sample was first dialyzed against 2 changes of 1000 volumes each of 50 mM EDTA, then against 2 changes of 1000 volumes of 2 mM 1,10-phenanthroline, and finally against 1000 volumes of metal-free 20 mM Tris pH 7.5. The sample, before and after the chelating treatment, was assayed for receptor-binding, metal content and apoptotic activity. Receptor binding was measured as described in Example 3, metal content was determined by ICP-AES and apoptotic activity was assayed as described in Example 4. ICP-AES showed that the dialysis procedure removed the bound zinc. After this treatment, the receptor affinity was significantly reduced (Table I) and the apoptotic activity was decreased 90-fold (FIG. 4).
Circular dichroic spectra were recorded on an AVIV instrument (Lakewood, N.J.) model 202 spectropolarimeter. The spectrum was scanned from 250 to 200 nm using a step size of 0.5 nm, an averaging time of 5 seconds, and quartz rectangular cuvettes having a pathlength of 1 cm. The protein concentration was 2 μM in solutions containing PBS. As shown in FIG. 6, Apo-2L (form 114-281) gives a CD spectrum typical of a protein having a high beta-sheet content. Removal of the bound zinc results in a decreased intensity of the dichroic peaks suggesting that the beta-sheet content has been diminished.
Circular dichroism was used to monitor the effect of zinc removal on the thermal stability of Apo-2L (form 114-281). These experiments also used 1 cm quartz rectangular cuvettes and a protein concentration of 2 μM. Circular dichroism at 225 nm was monitored as the sample was heated from 30 to 100° C. Measurements were taken at 2° C. increments after allowing the sample to equilibrate at that temperature for 1 minute. Both the ellipticity (CD) and dynode voltage were recorded and the temperature dependence of the dynode voltage is plotted in FIG. 7. The dynode voltage is proportional to the absorbance of the sample. An increase in dynode voltage upon heating of the sample reflects protein aggregation. The increase in dynode voltage was concomitant with a loss of secondary structure as indicated by a decrease in the ellipticity at 225 nm. These data suggest that Apo-2L (form 114-281) aggregates upon thermal denaturation with the midpoint for this transition (Tm) occurring at about 75° C. Removal of the bound zinc results in a large decrease in the Tm for thermal denaturation to about 54° C. These data show that the bound zinc is necessary for maintenance of the structure and stability of homotrimeric Apo-2L.
Example 7
Effects of Removal of Zinc Binding Site from Apo-2L by Mutation
Cys230 of Apo-2L (form 91-281) was replaced with Ala or Ser by using oligonucleotide-directed mutagenesis as described in Example 1. The variant proteins were then expressed and purified as described in Example 1. As shown in Table I, both the C230A and C230S mutants of Apo-2L (form 91-281) had reduced receptor-binding affinity and greatly diminished apoptotic activity. Since the Apo-2L x-ray structure shows that Cys230 is a buried residue, and thus is unlikely to directly contact receptor upon complex formation, these data suggest that mutation of Cys230 indirectly affects activity by changes in the structure or stability of homotrimeric Apo-2L.
Example 8
Additions of Zn Improves Soluble Apo-2 Ligand Product Accumulation and Recovery
A. Apo-2L (Amino Acid Residues 114-281) Expression Regulated by the Alkaline Phosphatase Promoter
pAPApo2-P2RU (see FIG. 12) encodes for the co-expression of Apo-2L (amino acid residues 114-281) and the tRNA's encoded by pro2 and argU. The pBR322-based plasmid [Sutcliffe, Cold Spring Harbor Symp. Quant. Biol., 43:77-90 (1978)] pAPApo2-P2RU was used to produce the Apo-2L in E. coli. The transcriptional and translational sequences required for the expression of Apo-2L are provided by the alkaline phosphatase promoter and the trp Shine-Dalgarno, as described for the plasmid phGH1 [Chang et al., Gene, 55:189-196 (1987)]. The coding sequence for Apo-2L (form 114-281) is located downstream of the promoter and Shine-Dalgarno sequences and is preceded by an initiation methionine. The coding sequence includes nucleotides (shown in FIG. 1) encoding residues 114-281 of Apo-2L (FIG. 1) except that the codon encoding residue Pro119 is changed to “CCG” instead of “CCT” in order to eliminate potential secondary structure. The sequence encoding the lambda to transcriptional terminator [Scholtissek et al., Nucleic Acids Res., 15:3185 (19.87)] follows the Apo-2L coding sequence. Additionally, this plasmid also includes sequences for the expression of the tRNA's pro2 [Komine et al., J. Mol. Biol., 212:579-598 (1990)] and argU/dnaY [Garcia et al., Cell, 45:453-459 (1986)]. These genes were cloned by PCR from E. coli w3110 and placed downstream of the lambda to transcriptional terminator sequence. This plasmid confers both tetracycline and ampicillin resistance upon the production host.
Strain 43E7 (E. coli W3110 fhuA(tonA) phoA Δ(argF-lac) ptr3 degP kanS ompT ilvG+) was used as the production host for the co-expression of the Apo-2 ligand and the tRNA's. Competent cells of 43E7 were prepared and transformed with pAPApo2-P2RU using standard procedures. Transformants were picked from LB plates containing 20 μg/ml tetracycline (LB+Tet20), streak-purified, and grown in LB broth with 20 μg/ml tetracycline in a 30° C. shaker/incubator before being stored in DMSO at −80° C.
A shake flask inoculum was prepared by inoculating sterile medium using a freshly thawed stock culture vial. Appropriate antibiotics were included in the medium to provide selective pressure to ensure retention of the plasmid. The shake flask medium composition is given in Table III. Flask cultures were incubated with shaking at about 30° C. (28° C.-32° C.) for 14-18 hours. This culture was then used to inoculate the production fermentation vessel. The inoculation volume was between 0.1% and 10% of the initial volume of medium.







TABLE III







Shake Flask Medium Composition










Ingredient
Quantity/Liter















Tetracycline
4-20 
mg



Tryptone
8-12
g



Yeast extract
4-6
g



Sodium chloride
8-12
g



Sodium phosphate, added as pH7
4-6
mmol



solution










Production of the Apo-2L was carried out in the production medium given in Table IV. The fermentation process was conducted at about 30° C. (28-32° C.) and pH controlled at approximately 7.0 (6.5-7.5). The aeration rate and the agitation rate were set to provide adequate transfer of oxygen to the culture. At the onset of product expression, induced by phosphate depletion, the process temperature was shifted from 30° C. to 25° C. Throughout the fermentation process, the cell culture was fed glucose based on a computer algorithm to meet its carbon requirement while ensuring aerobic condition.
Two batch additions of ZnSO4 were made during the fermentation process. One addition was made just prior to the induction of product expression. The second addition was made at approximately the mid-point of the production period. In this example, the additions occurred at a culture optical density of about 80-120 OD550 and at about 28 hours post-inoculation. Sufficient amounts of 100 mM ZnSO4 were added to achieve approximately 50-100 micromolar (final concentration) with each batch addition of the metal ions.
The fermentation was allowed to proceed for about 34-45 hours, after which the cell paste was harvested from the broth for subsequent product recovery evaluation.







TABLE IV







Production Medium Composition for AP Promoter 


Expression System










Ingredient
Quantity/Liter















Tetracycline
4-20
mg



Glucosea
10-250
g



Ammonium sulfatea
2-8
g



Sodium phosphate, monobasic,
1-5
g



dihydratea





Potassium phosphate, dibasica
1-5
g



Potassium phosphate, monobasica
0-5
g



Sodium citrate, dihydratea
0.5-5
g



Potassium chloride
0-5
g



Magnesium sulfate, heptahydratea
1.0-10
g



Antifoam
0-5
ml



Ferric chloride, hexahydratea
20-200
mg



Zinc sulfate, heptahydratea
0.2-20
mg



Cobalt chloride, hexahydratea
0.2-20
mg



Sodium molybdate, dihydratea
0.2-20
mg



Cupric sulfate, pentahydratea
0.2-20
mg



Boric acida
0.2-20
mg



Manganese sulfate, monohydratea
0.2-20
mg



Casein hydrolysatea
5-25
g



Yeast extracta
5-25
g







aA portion of these ingredients may be fed to the culture during the fermentation.



Ammonium hydroxide was added as required to control pH.






Broth samples were taken over the time course of the fermentation process. Cells from 1 ml of broth samples diluted to a cell density of 20 OD550 were collected by centrifugation and the resultant cell pellets were stored at −20° C. until analysis. The cell pellets were thawed and resuspended in 0.5 ml of extraction buffer (50 mM HEPES, pH 8.0, 50 mM EDTA and 0.2 mg/ml hen egg-white lysozyme) and mechanically disrupted to release the product from the cytoplasm. Solids were removed from the cell lysates by centrifugation before the clarified lysates were loaded onto a Dionex ProPac IEX HPLC column for trimer quantitation. The HPLC assay method resolves the product away from the contaminating E. coli proteins by use of a 5%-22% gradient of 1M NaCl in a 25 mM phosphate (pH 7.5) buffer over 25 minutes at a flow rate of 0.5 ml/min.
Frozen cell paste was thawed and resuspended in extraction buffer (100 mM Hepes buffer, pH 8.0, 50 mM EDTA, 5 mM DTT). After multiple passes of the cell suspension through a mechanical homogenizer to release the Apo-2L product from the cytoplasmic compartment, 0.2% PEI (final concentration) was added and the solids were removed by centrifugation. The clarified lysate was diluted 1:1 (v/v) with H20 and pH adjusted to 7.2 prior to loading onto the MPHS column (BioRad) pre-equilibrated with 3-4 column volumes of 50 mM Hepes/0.05% Triton/1 mM DTT pH 7.2. After a wash step with the 2-3 column volumes of equilibration buffer followed by a second wash step with 0.1M NaCl in the equilibration buffer, Apo-2L proteins were eluted off the MPHS column with 6 column volumes of a 0.1M to 0.8M NaCl gradient in the equilibration buffer. Column eluant fractions were collected, analyzed and the relevant fractions were pooled and stored @ 4-8° C.
Analysis of the Apo-2L accumulation during fermentation showed that the ZnSO4 additions did not significantly affect cell growth. The production of Apo-2 ligand began when the phosphate in the medium was depleted, typically about 15-25 hours after inoculation. FIG. 8 shows the time course in the accumulation of soluble Apo-2L trimers detected by the IEX HPLC method. Cultures with ZnSO4 additions had higher product concentration in the cell lysate samples than the minus-ZnSO4 controls.
Analysis of the product recovery at the initial capture step (involving IEX Chromatography) (FIG. 9) shows the elution profiles of the MPHS chromatography of cell lysates from fermentations conducted in the absence and presence of ZnSO4 additions. After the initial flow-through and wash steps, two main peaks, Peak A and Peak B, were resolved. By SDS-PAGE analysis, both peaks consisted of mainly Apo-2L.
Purified material from both peaks was prepared and analyzed for biological activity and stability. Results obtained from these studies suggested that Peak A was a more stable pool of Apo-2L product while Peak B had a greater tendency to aggregate over time. To minimize instability, Peak B was excluded for further recovery. The ratio of Peak A to Peak B was estimated by weighing the cut out traces representing the integrated area under each of the peaks. Results tabulated in Table V show a shift of the percent of Apo-2L as Peak A from approximately 45% on the average for the minus-ZnSO4 case to approximately 80% for the plus-ZnSO4 case, a significant increase in the amount of Apo-2L product in the recoverable pool.











TABLE V






ZnSO4 
Chromatography 
Peak 
Peak 


Run ID
Additions
Scale
A %
B %







SAPO2-113
No
0.66 × 15.5 cm
41.6
58.4


LAPO2-4
No
 4.4 × 41.5 cm
50.4
49.6


SAPO2-138
Yes
0.66 × 17.0 cm
78.8
21.2









B. Apo-2L (Amino Acid Residues 114-281) Expression Regulated by the trp Promoter:

PS1346.Apo2L.0 Plasmid Construction: DNA encoding residues 114-281 of Apo-2L (preceded by an initiating methionine codon) was inserted into a pS1346 plasmid vector. The pS1346 plasmid is a derivative of pHGH207-1 [DeBoer et al., Promoters: Structure and Function, Praeger, New York, pp. 462-481 (1982)] and contains the lambda-to transcriptional terminator [Scholtissek et al., Nucleic Acids Res., 15:3185 (1987)] downstream of the Apo-2L encoding sequence.
Strain 54C2 (E. coli W3110 fhuA(tonA) ion galE rpoHts(htpRts) clpP lacIq) was used as the production host for the expression of Apo-2 ligand (amino acid residues 114-281) where the ligand expression was regulated by the trp promoter. Competent cells of 54C2 were prepared and transformed with pS1346.Apo2L.0 using standard procedures. Transformants were picked from LB plates containing 20 μg/ml tetracycline (LB+Tet20), streak purified, and grown in LB broth with 20 μg/ml tetracycline in a 30° C. shaker/incubator before being stored in DMSO at −80° C.
Experiments conducted with the production organism, 54C2/pS1346.Apo2L.0, were performed under similar fermentation parameters as described above for the AP-promoter expression system except for minor adjustments in the medium composition (Table VI), the addition of an inducer, indole3-acrylic acid (IAA), and the length of the process. Tryptophan was added to the initial medium to repress promoter activity during the initial growth phase. The temperature shift from 30° C. to 25° C. was made when cell density of the broth reached approximately 30 OD550. The inducer was added when the cell density of the broth reached approximately 55 OD550. In the experiments where ZnSO4 additions were made, sufficient amounts of 100 mM ZnSO4 solution were added at cell density of 25 OD550 and at 24 hours post-inoculation to achieve a final concentration of approximately 50-100 μM. Cell pastes were harvested at 6 hours post inducer addition and stored at −20° C. to −80° C.







TABLE VI







Additions to the Production Medium for AP


Promoter Expression System necessary for 


the trp Promoter Expression System










Ingredient
Quantity/Liter







L-isoleucine
0.5-1 g



Tryptophan
0.1-5 g










Cell growth for the trp promoter system slowed after reaching 40-60 OD550. There was significant leaky expression of Apo-2 ligand prior to induction with IAA addition and was probably responsible for the growth problem. Cell growth profiles were comparable in the absence and the presence of ZnSO4 additions. FIG. 10 shows the time course in the accumulation of soluble Apo-2L trimers detected by the IEX HPLC method. The accumulation of soluble Apo-2L continued to increase in the run with ZnSO4 additions and achieved a higher product concentration in the cell lysate samples.
C. Apo-2L (amino acid residues 114-281) recovery and purification from E. coli using divalent metal ions/DTT:
The following protocol may be employed in recovery and purification of Apo-2L from E. coli. First, the cells are homogenized and extraction is performed as follows. Frozen harvested Escherichia coli cells are suspended in 6 volumes of extraction buffer (100 mM HEPES/5 mM DTT (or 5 mM Zn sulfate instead of DTT), pH 8.0), or whole cell broth is conditioned with 5 mM DTT (or 5 mM Zn sulfate instead of DTT) @ pH 8.0. The suspension is thoroughly mixed for 1 hour at 2-8° C., then homogenized in a homogenizer (Gaulin Corporation, Everett, Mass.). The broken cell suspension is flocculated in 0.2% PEI for 1-2 hours and centrifuged by a BTPX205 (Alfa Laval Separation AB, Sweden) continuous feed centrifuge and clarified by depth filtration.
After extraction, the Apo-2L is purified as follows. Macro-Prep ceramic High S (MP-HS) chromatography is performed by conditioning the clarified cell suspension (extract) with an equal volume of H2O/0.1% Triton-100 and adjusted pH to 7.2. The conditioned extract is loaded onto a column of MP-HS cation exchanger (Bio-Rad, Hercules, Calif.) that is equilibrated in 50 mM HEPES/0.05% Triton-100/1 mM DTT (or 100 uM Zn sulfate), pH 7.2. (In the preceding two steps, SP-Sepharose Fast Flow (Amersham Pharmacia, Sweden) may alternatively be employed). The non-binding proteins are flowed through and removed by washing with equilibration buffer to baseline @ A280. The column is washed with 3 column volumes of 0.1 M NaCl/equilibration buffer. The Apo-2L is eluted using a linear, 8 column-volume gradient from 0.1 to 0.8M sodium chloride in equilibration buffer. Fractions are collected and those which contain properly-folded Apo-2L, as determined by SDS-PAGE or SEC-HPLC assay, are pooled.
The pool of Apo-2L from the MP-HS column is loaded onto a column of Macro-Prep Hydroxyapatite (Bio-Rad, Hercules, Calif.) equilibrated in 50 mM HEPES/1 mM DTT (or 100 uM Zn sulfate), pH 7.2. (As an alternative to the Macro-Prep Hydroxyapatite, SP-Sepharose Fast Flow may be employed). After the sample is loaded, the column is washed with equilibration buffer to baseline @ A280. The Apo-2L is eluted out of the column by using an isocratic step of 0.15 M sodium phosphate in equilibration buffer.
The pool of MP-HA is conditioned with an equal volume of 1.0 M Ammonium Sulfate/50 mM Tris/1 mM DTT (or 100 uM Zn sulfate), pH 7.5, and then loaded onto a column of Phenyl-Sepharose FF (Amersham Pharmacia, Sweden) that is equilibrated in 0.5 M Ammonium sulfate/50 mM Tris/1 mM DTT (or 100 uM Zn sulfate), pH 7.5. (As an alternative to the 0.5M ammonium sulfate, 0.6M sodium sulfate may be employed). The column is washed with equilibration buffer, and the Apo-2L is collected in the column effluent.
The Apo-2L is then formulated by ultrafiltration and G-25 Gel Filtration (Amersham Pharmacia, Sweden) chromatography. The pool of phenyl-Sepharose is concentrated with TFF Ultrafiltration (Millipore, Bedford, Mass.) and formulated on a G-25 gel filtration column with 20 mM Tris/8% Trehalose, pH 7.5. (As an alternative, the material may be formulated by diafiltration). The final purity of Apo-2L can be determined by SDS-PAGE, SEC-HPLC and Amino Acid sequence analysis.
Example 9
Additions of Cobalt Chloride Improve Soluble Apo-2 Ligand Product Accumulation and Recovery
Apo-2L (Amino Acid Residues 114-281) Expression Regulated by the Alkaline Phosphatase Promoter
The same production organism, medium composition, fermentation conditions and sample analysis described in Example 8A were used in studying the effect of additions of metal ion other than Zn, namely cobalt chloride, on Apo-2L (amino acid residues 114-281) product accumulation. A solution of 100 mM CoCl2 in H2O was used in place of the 100 mM ZnSO4 and sufficient amounts were added to arrive at a final concentration of 50-100 μM at each of the two additions.
FIG. 11 shows the benefits of additions of CoCl2 on soluble Apo-2L accumulation during the fermentation process. Like the ZnSO4 addition experiments, though not as significant, a higher accumulation rate of soluble Apo-2L product was detected by the IEX HPLC assay method. The data demonstrates that additions of certain metal ions generally improve soluble Apo-2L accumulation, likely as a result of their ability to stabilize the assembled trimers of Apo-2L.
Example 10
Effects of Various Metal Ions on Apo-2L Formulations
In vitro assays were conducted by incubating 50 μl Apo-2L (form 114-281) at 5° C. for 24 hours with 5 mM of metal salt (each of which are recited in Table VII below; 100:1 molar ratio of metal to protein) in a 20 mM Tris, 8% trehalose, 0.01% Tween 20, pH 7.5 formulation. The samples were then evaluated for apoptotic activity using a SK-MES assay as described in Example 4.
The EC50 (or Apo-2L concentration that results in killing of 50% of the cells) is shown in Table VII (units of ng/ml). As shown in Table VII, addition of Zn acetate and Zn sulfate to the culture enhanced Apo-2L activity.










TABLE VII







Metal Added
Bioassay EC50









Control
19.0 ± 1.9 (n = 3) 



Mn acetate
19.8



Mn chloride
19.9



Fe acetate
32.5



Co acetate
25.9



Co chloride
17.9



Co sulfate
16.7



Ni acetate
18.7



Cu acetate
844



Cu chloride
984



Cu sulfate
774



Ag acetate
20



Zn acetate
12.3



Zn chloride
17.2



Zn sulfate




(100:1)
9.3 ± 1.2 (n = 3)



 (10:1)
10.0 ± 0 (n = 2)  



 (1:1)
11.5 ± 0.7 (n = 2) 










Example 11
Effects of Zinc Metal Ions on Apo-2 Ligand Formulation Stability
As indicated in Example 8B, the first, step in the purification process using MPHS cation exchanger gives two peaks, A and B. To investigate the storage stability of the two peaks, Apo-2L (form 114-281) was extracted from the E. coli expressed product (using the pS1346.ApoL.0 plasmid with trp promoter), and purified by MPHS cation exchanger. The MPHS Peak A was further purified by MP-Hydroxyapatite and Phenyl-Sepharose FF, and then formulated using G-25 gel filtration. The MPHS Peak B was purified by MP-Hydroxyapatite, Phenyl-Sepharose FF, and Ni-NTA Superflow and then formulated on G-25 gel filtration into 20 mM Tris, 8% trehalose, 0.01% Tween 20, pH 7.5. The samples were sterile filled into 3 cc glass vials and sealed with teflon coated greybutyl stoppers. The storage stability of the purified and formulated Peaks A and B were evaluated at the time (weeks (“wk”) or months (“mo”)) and temperatures (° C.) listed in Table VIII.













TABLE VIII






SDS-SEC
SDS-SEC
SEC %
SEC %
RP %




% M
% D
recovery
trimer 
main
EC50





















Peak A








1 wk, 
ND
ND

98.60
78.10



−70 C.








1 wk, 
ND
ND
96.7
98.2
73.60



37 C.








2 mo, 
88.85
7.50

93.93
74.52



−70 C.








2 mo, 
84.90
10.58
65.07
94.03
53.98



30 C.








6 mo, 
93.00
5.80


ND
11.1


−70 C.








6 mo, 
87.00
9.44
100

ND
14.8


5 C. 








6 mo, 
80.00
8.68
76

ND
67.8


30 C.








Peak B








1 wk, 
ND
ND

98.70
75.3



−70 C.








1 wk, 
ND
ND
101.1
98.3
61.4



37 C.








2 mo, 
94.03
3.79

90.00
65.77



−70 C.








2 mo, 
94.12
4.54
46.07
94.12
32.185



30 C.








6 mo, 
82.00
11.25


ND
10.3


−70 C.








6 mo, 
73.50
17.00
92

ND
12.6


5 C.








6 mo, 
47.00
23.20
38

ND
Dead


30 C.









Table VIII shows, among other things, the percent monomer (% M) and percent dimer (% D); “ND” refers to those values not determined. Peak A included slightly less dimer and other impurities than Peak B at Day 0. As shown in Table VIII, Peak B was found to be more thermally labile, as assessed by the more extensive precipitation at 30° C. (approximately 40% more at the 2 month point). Even at 5° C., Peak B was approximately 2-fold more prone to dimer formation than Peak A. Biochemical analysis of Peaks A and B indicated that the material of each was biochemically equivalent except for their respective stability properties. It is believed that the lower stability of Peak B may be related to improper (trimer) assembly or lower zinc content (0.83 molar ratio of Zinc to protein for Peak A and 0.7 molar ratio of Zinc to protein for Peak B).

## Claims

1. A method of making Apo-2 ligand, comprising the steps of: (a) providing an E. Coli host cell comprising a replicable vector containing a nucleotide sequence encoding Apo-2 ligand polypeptide; (b) providing culture media containing zinc at a concentration of about 50 micromolar to about 250 micromolar; (c) culturing the host cell in the culture media under conditions sufficient to express the Apo-2 ligand; and (d) recovering the Apo-2 ligand from the host cell or culture media, wherein
the Apo-2 ligand comprises a polypeptide selected from the group consisting of:
(i) a polypeptide having amino acid residues 1 to 281 of FIG. 1 (SEQ ID NO:1);
(ii) a polypeptide having amino acid residues 114 to 281 of FIG. 1 (SEQ ID NO:1);
(iii) a fragment of the polypeptide of (i) or (ii) which induces apoptosis in at least one type of mammalian cell or binds an Apo-2 ligand receptor; and
(iv) a polypeptide having at least 80% identity to the polypeptide of (i) or (ii), and induces apoptosis in at least one type of mammalian cell or binds an Apo-2 ligand receptor.

2. The method of claim 1 wherein said zinc comprises zinc sulfate.

3. The method of claim 1 wherein said replicable vector comprises a nucleotide sequence encoding one or more tRNA molecules.

4. The method of claim 3 wherein said replicable vector is the pAPApo2-P2RU vector.

5. The method of claim 1 wherein said Apo-2 ligand comprises amino acids 114 to 281 of FIG. 1 (SEQ ID NO:1).

6. The method of claim 1 wherein said Apo-2 ligand comprises amino acids 1 to 281 of FIG. 1 (SEQ ID NO:1) or a fragment thereof which induces apoptosis in at least one type of mammalian cell.

7. The method of claim 1, wherein in step (d), the Apo-2 ligand is recovered from the host cell or culture media in the presence of a reducing agent.

8. The method of claim 7, wherein said reducing agent is selected from the group consisting of DTT and BME.