# STUDY NO. 00-4205

SPONSOR STUDY NO. 211-DIPE-1G

GASOLINE DIPE VAPOR CONDENSATE

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION

TOXICITY STUDY IN RATS

Final Report

Submitted to: American Petroleum Institute

1220 L Street, Northwest

Washington, D.C. 20005-4070

Attn: Thomas M. Gray, M.S., D.A.B.T.

Date: 4 August 2011

Page 1 of 636

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STATEMENT OF COMPLIANCE

This study was conducted in compliance with the United States Environmental Protection

Agency's Good Laboratory Practice Standards 79.60, CFR Vol. 59, No. 122, 27 June

1994 with the following exception:

It was the Sponsor’s responsibility to maintain the method of synthesis, fabrication, or

derivation of the test fuel, and this was not completed at the time of the study conduct but

has been completed since and is on file with the Sponsor.

Gary M. Hoffman, B.A., D.A.B.T. Date

Study Director

Thomas M. Gray, M.S., D.A.B.T. Date

Sponsor Representative

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SIGNATURE PAGE

SCIENTISTS

The following Scientists were responsible for the overall conduct of this study.

Departmental supervisory personnel are listed on the personnel page of this report

(Appendix EE ).

Gary M. Hoffman, B.A., D.A.B.T. Date

Stu dy Director

Kevin Keane, D.V.M., Ph.D. Date

Director of Pathology 1



# SCIENTIFIC REVIEW

The following Scientists have reviewed and approved this report.

Robert M. Parker, Ph.D., D.A.B.T. Date

Director, Developmental and Reproductive Toxicology

Sylvie J. Gosselin, D.V.M., Ph.D., Diplomate A.C.V.P. Date

Vice President, Safety Assessment

1 

## Katharine M. Whitney was the Study Pathologist for this study and for submission of the draft report and is

no longer employed at the Testing Facility. Kevin Keane is assuming responsibility for finalization of the

pathology evaluation of this report.

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QUALITY ASSURANCE STATEMENT

Listed below are the dates that this study was inspected by the Quality Assurance Unit of

Huntingdon Life Sciences, East Millstone, New Jersey, and the dates that findings were

reported to the Study Director and Management. This report reflects the raw da ta as far

as can be reasonably established.

Type of Inspection Date(s) of

Inspection Reported to

Study Director

and Management

Pathology Associates Facility

Inspection 24 Apr 01 02 Nov 01

GLP Protocol Review 01 Apr 02 01 Apr 02

Exposure, Monitoring and Equipment

Records 20 May 02 20 May 02

Body and Feeder Weights 17 Jun 02 17 Jun 02

Estrous Determination 09 Jul 02 09 Jul 02

Litter Checks and Daily Viability

Check 20 Aug 02 20 Aug 02

Male Necropsy and Sperm

Assessment 10 Sep 02 11 Sep 02

Maternal & Pup Necropsy and

Training Records 17 Sep 02 17 Sep 02

Final Analytical Report and Study

Data 7-10, 20 and 21 Jan

03 21 Jan 03

Final Report Draft and Study Data 09, 10, 13-17, 20-

24, 26, 27 Jan 03 27 Jan 03

Final Report Review 04-17 Feb 10 17 Feb 10

Lesley Piccoli Date

Quality Assurance Auditor

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Gasoline DIPE Vapor Condensate: A One- Generation

Whole-Body Inhalation Reproduction Toxicity Study in Rats

SUMM ARY

This study was designed to assess the potential reproductive toxicity of Gasoline DIPE

Vapor Condensate when administered via whole

- body inhalation exposures to Sprague

Dawley rats (26/sex/group). The test substance was administered (using a nitrogen

enri ched air mixture) at target concentrations of 2000, 10000 and 20000 mg/m 3 

# for

6 hours/day, 7 days/week for 10 weeks before mating, for up to 2 weeks during mating,

for 3 weeks during gestation and for 4 weeks during lactation prior to weaning. In

addition , a control group (26/sex) received nitrogen enriched air only while in chamber.

Exposure levels were determined using an infra

- red spectrophotometer 4 times per

chamber per day. Additionally, the test substance’s major components were assayed once

per chamber per week. Particle size distribution measurements were also made once per

chamber per week using a TSI Aerodynamic Particle Sizer.

Viability checks were performed twice daily to check for mortality and signs of severe

toxic or pharmacologic effect s. Physical observations and body weight measurements

were made twice pretest and at least weekly during the study. Feed consumption

measurements were obtained beginning the week prior to treatment initiation and at least

weekly during the study. After completion of ~ 16 weeks of exposures, all parental male

animals (P 0 

# generation) were sacrificed. All parental female animals (P 0 

# generation)

were sacrificed on Lactation Day 28. Selected organs were weighed and organ/body

weight and organ/brain weight r atios calculated. Complete macroscopic postmortem

examinations were performed on all parental animals. Histopathological evaluations of

selected tissues were conducted on randomly selected parental animals.

Pups (F 1 

# generation) were observed as soon as p ossible after delivery for their sex, the

number of live and dead pups and pup abnormalities. Thereafter, litters were observed

twice daily. On the 4 th 

# day after parturition, litters with more than ten pups were

randomly culled to ten pups with sex distr ibution equalized when possible. Pups were

given a gross physical examination, were weighed and were sexed frequently until

weaning on the 28 th 

# day after parturition at which time they were sacrificed. A

macroscopic postmortem evaluation and the weighing of selected organs were performed

on randomly selected pups.

The mean ( ± standard deviation) analytical exposure concentrations of Gasoline DIPE

Vapor Condensate were determined to be 0 ± 0, 2034 ± 128, 10260 ± 621 and

20230 ± 775 mg/m 3 

# for the Air Contr ol and the exposure groups, respectively. The

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SUMMARY

analytically measured exposure levels of the airborne test substance were acceptably close

to the targeted exposure levels. The measured and nominal concentrations varied

somewhat (less than 3%), but accepta bly, from the expected 1:1 ratio for this type of

vapor exposure. Chamber environmental conditions averaged 25oC temperature and 46%

relative humidity. Particle sizing results indicated that the atmospheres were essentially

vapor only, as expected, since there was no substantial difference between the particulate

levels in the test substance chambers and the Air Control chamber. Analysis of the major

components in the neat test substance and the test atmospheres showed an acceptably

close comparison betw een the neat test substance and the vaporized test substance. This

data demonstrated that the test animals were exposed, as expected, to all of the major

components of the test substance in their acceptably proper proportion. The data was

consistent from week

- to

- week during the study indicating stability of the test substance

and the atmosphere generation techniques.

There was no effect of treatment on survival, clinical observations, body weight gain,

feed consumption, estrous cycle (as measured by cycle length and number of estrous

cycles), mating indices for the male rats and mating or fertility or gestation indices for the

female rats. There were also no treatment

- related differences in the other reproductive

parameters up to the time of parturition i ncluding the percent of females completing

delivery and the duration of gestation, when compared to the Air Control group. There

were also no treatment

- related differences in all parturition parameters including the total

number of pups delivered, the number of pups dying, the viability (4 day survival) and

lactation (21 day survival) indices, the number of implantation sites per litter, the pup sex

ratio and the number of live pups/litter, when compared to the Air Control group. Mean

percent motility, ep ididymal sperm count, homogenization

- resistant testicular spermatid

count, sperm morphology and caudal epididymis weight were not affected by treatment

with the test substance at an exposure level of 20000 mg/m 3



# .

There were no exposure

- related differences in terminal estrous evaluations in the test

substance exposed animals compared to the Air Control animals. Scattered macroscopic

postmortem observations such as lung discoloration were noted in a treatment

- related

pattern, but in absence of any effects on lung weights or histopathology, were not

considered adverse findings. Exposure

- related effects on organ weights included

statistically significant increases in liver weights (absolute and/or relative to body weight

or brain weight) at the 2 higher exposu re levels in the males. Exposure

- related effects on

organ weights also included statistically significant increases in kidney weights (absolute

and/or relative to body and brain weight) at the 2 higher exposure levels in the males.

These differences for the males were consistent with the hydrocarbon nephropathy

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SUMMARY

findings at these exposure levels in the 13

- week inhalation study with this same test

substance. This species

- and gender

- specific change has been well documented in male

rats exposed to a variety of hydrocarbon compounds and is not considered relevant to

humans. No test substance related microscopic changes were noted in male and female

reproductive organs or other protocol- specified tissues in this study.

The pups were unremarkable for clinical observations and body weights and weight gains

during the lactation period. There were no exposure

- related differences in macroscopic

postmortem evaluations and organ weights in the pups feeding from test substance

exposed animals compared to the pups fee ding from Air Control animals.

In conclusion, exposure of rats to 2000, 10000 and 20000 mg/m 3 

# of vapor of Gasoline

DIPE Vapor Condensate resulted in no effects on the exposed parental animals prior to

their termination. However, increases in liver weights in parental male animals exposed

to 10000 and 20000 mg/m 3 

# of vapor were seen. Increases in kidney weights in parental

male animals exposed to the two higher exposure levels of vapor were consistent with

hydrocarbon nephropathy seen in similar animals exp osed for 13 weeks to this test

substance. However, this finding has been generally accepted (US EPA 1991. Alpha 2

microglobulin: association with chemically induced renal toxicity and neoplasia in male

rat. In Risk Assessment Forum, p.85. US Govt. Printing Office, Washington, DC.) not to

be relevant to human risk assessment. Therefore, a no observable effect level (NOEL) of

2000 mg/m 3 

# was determined. There was no effect at any of the exposure levels on

reproductive performance in the study, including ma ting, fertility, parturition, lactation

and offspring survival and development for 28 days after birth. Therefore, with respect to

reproductive performance, a no observable effect level ( NOEL) of 20000 mg/m 3 

# was

determined

.

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TABLE OF CONTENTS

COVER PAGE .................................................................................................................... .1

STATEMENT OF COMPLIANCE .....................................................................................2

SIGNATURE PAGE ...........................................................................................................3

QUALITY ASSURANCE STATEMENT ..........................................................................4

SUMMARY ....................................................................................................................... ..5

TABLE OF CONTENTS .....................................................................................................8

1. 

# INTRODUCTION ................................................................................................11

2. 

# MATERIALS AND METHODS

2.1. Study Management ....................................................................................11

2.2. Study Dates ................................................................................................11

2.3. Experimental Outline .................................................................................13

2.4. Test Substance ...........................................................................................14

2.5. Test Animals ..............................................................................................15

2.6. Animal Assignment ...................................................................................16

2.7. Animal Identification .................................................................................17

2.8. Veterinary Care ..........................................................................................17

2.9. Animal Husbandry During Non-Exposure Periods ...................................17

2.10. Test Substance Administration and Chamber Operation ...........................20

2.11. Experimental Ev aluations ..........................................................................20

2.12. Mating, Gestation and L actation Procedures .............................................21

2.13. Postmortem ................................................................................................23

2.14. Sperm Count, Motility and Morphology Assessments ..............................27

2.15. Statistical An alysis .....................................................................................28

2.16. Data Storage ...............................................................................................30

2.17. Regulatory References ...............................................................................30

2.18. Protocol Deviations ....................................................................................31

3. 

# RESULTS AND DISCUSSION

3.1. Chamber Monitoring ..................................................................................33

3.2. Parental Data ..............................................................................................35

3.3. Pup Data .....................................................................................................38

3.4. Postmortem Data ........................................................................................39

4. 

# CONCLUSION ....................................................................................................41

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TABLE OF CONTENTS

REFERENCES ..................................................................................................................42

CALCULATIONS .............................................................................................................43

GENERAL PREFACE ......................................................................................................44

TABLES

1. Summary of Survival and Pregnancy ........................................................................... 45

2. Summary of Clinical Observations - (Premating/Mating/Postmating) ........................ 46

3. Summary of Clinical Observ ations During Gestation .................................................. 52

4. Summary of Maternal Clinical Ob servations During Lactation .................................. 53

5. Mean Body Weight Values – (P remating/Mating/Postmating) ................................... 54

6. Mean Body Weight Gain - (Pre mating /Mating/Postmating) ...................................... 59

7. Summary of Gestation Body Weights .......................................................................... 64

8. Summary of Gestation Body Weight Gain ................................................................... 65

9. Summary of Maternal Lactation Body Weights ........................................................... 66

10. Summary of Maternal Lacta tion Body Weight Gain ................................................... 67

11. Mean Feed Consumption (grams/kg/day) - (Premating/Postmating) ......................... 68

12. Summary of Gestation Feed Consumption (grams/kg/day) ........................................ 72

13. Summary of Lactation Feed Consumption (grams/kg/day) ........................................ 73

14. Summary of Estrous Stages ........................................................................................... 74

15. Summary of Cohabitation Data ..................................................................................... 75

16. Summary of Delivery and Litter Data .......................................................................... 76

17. Summary of Pup Clinical Observations During Lactation ........................................... 80

18. Summary of Mean Pup Body Weights and Body Weight Gain ................................... 81

19. Summary of Parental Necropsy Observations .............................................................. 87

20. Summary of Pup Necropsy Observations ..................................................................... 92

21. Summary of Parental Organ Weights ........................................................................... 99

22. Summary of Pup Organ Weights ................................................................................ 112

23. Lesion Incidence Summary with Expanded Severity Levels ..................................... 116

APPENDICES

A. Inhalation Report ......................................................................................................... 121

B. Analytical Report ......................................................................................................... 173

C. Individual Animal Term ination History ..................................................................... 203

D. Individual Clinical Observations - (Premating/Mating/Postm ating) ......................... 211

E. Individual Clinical Observa tions During Gestation ................................................... 233

F. Individual Maternal Clinical Ob servations During Lactation .................................... 241

G. Individual Body Weights - (Prema ting/Mating/Postmating) ..................................... 253

H. Individual Body Weight Gain - (P remating/Mating/Postmating) .............................. 270

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TABLE OF CONTENTS

I. Individual Gestati on Body Weights ............................................................................ 283

J. Individual Gestation B ody Weight Gain .................................................................... 288

K. Individual Maternal Lact ation Body Weights ............................................................ 293

L. Individual Maternal Lacta tion Body Weight Gain ..................................................... 298

M. Individual Feed Consumption (grams/kg/day) - (Premating/Postmating) ................. 303

N. Individual Gestation Feed Cons umption (grams/kg/day) .......................................... 312

O. Individual Maternal Lactation Feed Consumption (grams/kg/day) ........................... 317

P. Individual Estrous Cycl e and Mating Data ................................................................. 322

Q. Individual Mating Assignments .................................................................................. 330

R. Individual Delivery a nd Litter Data ............................................................................ 334

S. Individual Pup Sex and Stat us During Lactation ........................................................ 338

T. Individual Pup Clinical Observ ations During Lactation ............................................ 342

U. Individual Pup Body Weights ..................................................................................... 382

V. Individual Parental Necr opsy Observations ............................................................... 406

W. Individual Pup Necrops y Observations ...................................................................... 429

X. Individual Parental Organ Weights ............................................................................. 462

Y. Individual Pup Organ Weights .................................................................................... 491

Z. Individual Animal Gross and Mi croscopic Observations .......................................... 508

AA. Sperm Analysis Report ...........................................................................................550

BB. Certificates of Analysis – (Feed, Water and Bedding) ...........................................564

CC. Protocol and Protoc ol Amendments .......................................................................598

DD. Historical Control Data ...........................................................................................630

EE. Testing Facility Personnel ........................................................................................... 635

FF. Report Amendments .................................................................................................... 636

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1. INTRODUCTION

This reproductive study was designed to provide general information concerning

the effects of Gasoline DIPE Vapor Condensate on the integrity and performance

of the male and female reproductive systems, including gonadal function, the

estrous cycle, mating behavior, conception, gestation, parturition and lactation,

and to provide information about the effects of the test substance on neonatal

morbidity and mortality, and data on prenatal and postnatal developmental

toxicity.

2. MATERIALS AND METHODS

2.1. STUDY MANAGEMENT

2.1.1. SPONSOR

American Petroleum Institute (API)

1220 L Street, Northwest

Washington, DC 20005-4070

2.1.2. SPONSOR REPRESENTATIVE

Thomas M. Gray, M.S., D.A.B.T.

2.1.3. TESTING FACILITY

Huntingdon Life Sciences

P.O. Box 2360

Mettlers Road

East Millstone, Ne w Jersey 08875-2360

2.1.4. STUDY DIRECTOR

Gary M. Hoffman, B.A., D.A.B.T.

2.2. STUDY DATES

2.2.1. STUDY INITIATION

6 May 2002 (Date Study Director signed the Protocol)

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2.2.2. DATE OF ANIMAL RECEI PT

6 May 2002

2.2.3. EXPOSURE INITIATION

20 May 2002 (Experimental Start Date)

2.2.4. MATING INITIATI ON

28 July 2002

2.2.5. EXPOSURE TERMINATION

23 September 2002

2.2.6. TERMINAL SACRIFICE

P 0 

# Males: 10 – 11 September 2002

P 0 

# Females: 23 August 2002 – 24 September 2002

2.2.7. EXPERIMENTAL TERMINA TION DATE

4 August 2011 (Date of last data collection = Date Pathologist

signed the F inal Report)

2.2.8. STUDY COMPLETION

4 August 2011 (Date Study Director signed the Final Report)

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2.3. EXPERIMENTAL OUTLINE

Group Group

Designation Exposure

Levels

(mg/m 3 

# ) a 

# Number of Animals

Mated Adults Microscopic

Pathology Adult

Generations b 

# Macroscopic Postmortem

Examinations

- Pups P 0 

# P 0



# M F M F F 1



# I Control 0 (air only) 26 26 10 10 3/sex/litter

II Low 2,000 26 26 0 0 3/sex/litter

III Middle 10,000 26 26 0 0 3/sex/litter

IV High 20,000 26 26 10 10 3/sex/litter

a 

# Exposures daily (7 days/week) for 6 hours per day.

b 

# Histologic examinations were performed on reproductive and select tissues for the control and high-exposure

animals.

M = Male; F = Female; The first day of exposure was Day 0.

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2.4. TEST SUBSTANCE

Gasoline DIPE Vapor Condensate

2.4.1. SUPPLIER

Chevron Texaco Energy Research and Technology Company

100 Chevron Way

Richmond, CA 94802

2.4.2. LOT NUMBER

API -01-06

2.4.3. PURITY/ANALYTICAL CONCENTRATION

100% Gasoline DIPE Vapor Condensate

2.4.4. DESCRIPTION

Colorless Liquid

2.4.5. DATES RECEIVED

7 December 2001 & 5 August 2002

2.4.6. EXPIRATION DATE

Not available; stable per MSDS.

2.4.7. ANALYSIS

Documentation of the identity, strength, purity, composition,

stability, and method of synthesis, fabrication, and/or derivation of

the test article and the maintenance of these records was the

resp onsibility of the Sponsor. The Sponsor conducted a purity

analysis of the test substance by GC prior to the start of this study.

2.4.8. STORAGE

Ambient conditions in an outdoor solvent shed or in an indoor

laboratory.

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2.4.9. DISPENSING

The test substance was received fr om Chevron Texaco Energy

Research and Technology Company in 100 gallon cylinders. Since

only 5

- gallon cylinders were practical to be used for exposure

operations, the test substance was dispensed, as needed, at the

Testing Facility from the 100

- gallon cyl inders into 5 -gallon

cylinders using nitrogen pressurization.

2.4.10. ARCHIVAL SAMPLE

An archival sample from test substance is stored in the Archives of

the Testing Facility under conditions specified for test substance

storage.

2.4.11. DISPOSITION

The unused portion of the test substance as well as any empty test

substance containers was returned to the Sponsor on 11 January

2007 . Empty test substance containers were returned on an as

needed basis. The Sponsor was responsible for tracking their

disposition.

2.5. TEST ANIMAL S

Albino Rats (Outbred) VAF/Plus 



# 2.5.1. SPECIES

Sprague- Dawley derived CD 



# [Crl: CD  

# IGS BR]

2.5.2. SUPPLIER

Charles River Laboratories

Kingston, New York 12484

2.5.3. JUSTIFICATION FOR TE ST ANIMAL SELECTION

The rat was used as a surrogate for humans to detect adverse

reprod uctive effects and is a species in which known reproductive

toxicants have been detected. The rat is a rodent animal model

commonly utilized in reproduction studies and is recommended in

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the referenced guidelines. In addition, a historical control databa se

with this strain of animal and supplier facility is available for

comparative evaluation.

2.5.4. NUMBER OF ANIMALS

Received:

220 total (110 males, 110 females)

Placed on test:

208 total (104 males, 104 females)

Females were nulliparous and non-pregnant.

2.5.5. AGE AT RECEIPT

Approximately 28 days.

2.5.6. AGE AT INITIATION OF EXPOSURES

Approximately 42 days.

2.5.7. WEIGHT AT INITIATION OF EXPOSURES (GRAMS)

Mean Range

Male: 213 186 - 245

Female: 153 130 - 179

Individual weights of animals placed on test were within ± 20% of

the mean weight for each sex.

2.5.8. ACCLIMATION PERIOD

Animals were acclimated for 14 days. All animals were examined

during the acclimation period to confirm suitability for study.

2.6. ANIMAL ASSIGNMENT

More animals than required for the study were purchased and accli mated.

Animals considered suitable for the study on the basis of pretest body

weight data and pretest physical examinations, were randomly assigned, by

sex, to control or treated group, in an attempt to equalize mean group body

weights.

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2.7. ANIMAL IDENTIFICAT ION

Each rat was assigned a temporary identification number upon receipt.

After selection for study (P 0 

# generation), each rat was identified with a

metal ear tag bearing its assigned animal number. The assigned animal

number plus the study number compris ed the unique animal number for

each animal. If the tag was lost, it was replaced. In addition, each cage

was provided with a cage card that was color

- coded for exposure level

identification and contained the study number and animal number

information.

2.8. V ETERINARY CARE

Animals were monitored by the technical staff for any conditions requiring

possible veterinary care and treated as necessary. Miscellaneous, non

- test

article

- related veterinarian evaluations and treatments (as necessary) for

individual anim als were reviewed by the study director and are

documented in the study file.

2.9. ANIMAL HUSBANDRY DURING NON

- EXPOSURE PERIODS

2.9.1. FACILITIES MANAGEMENT/ANIMAL HUSBANDRY

Currently acceptable practices of good animal husbandry were

followed e.g., Guide for the Care and Use of Laboratory Animals ;

National Academy Press, 1996. Huntingdon Life Sciences, East

Millstone, New Jersey is fully accredited by the Association for

Assessment and Accreditation of Laboratory Animal Care

International (AAALAC).

2.9.2. HOUSING

Animals we re housed in suspended, stainless steel cages with wire

mesh fronts and floors. Animals were doubly housed during the

initial week of acclimation and individually housed thereafter,

except as follows.

Mating: One male and one female were co

- housed conti nuously

(except during treatment) for 14 days, or until mating occurred.

Lactation: Dam and litter were housed together in a solid plastic

“shoebox” cage until weaning.

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2.9.3. FEED

Certified Rodent Diet, No. 5002; (Meal) (PMI Nutrition

International, St. Louis, Missouri) was available without restriction

except during exposures.

2.9.4. FEED ANALYSIS

Analysis of each feed lot used during this study was performed by

the manufacturer. Results can be found in Appendix BB. There

were no known contaminants in the feed, wh ich were expected to

interfere with the results of this study.

2.9.5. WATER

Water (Elizabethtown Water Company, Westfield, New Jersey)

was available without restriction, except during exposures, via an

automated watering system.

2.9.6. WATER ANALYSIS

Water analyses are conducted by Elizabethtown Water Company,

Westfield, New Jersey (Raritan

- East Millstone Plant) to ensure that

water meets standards specified under the EPA Federal Safe

Drinking Water Act Regulations (40 CFR Part 141). In addition,

water samples are colle cted biannually from representative rooms

in the Testing Facility; chemical and microbiological water

analyses are conducted on these samples by a subcontract

laboratory. Results of all water analyses can be found in Appendix

BB. There were no known cont aminants in the water that were

expected to interfere with the results of this study.

2.9.7. BEDDING SUBSTANCE

Ground corncob bedding (Bed

- O’ -Cobs  

# 1⁄4 inch irradiated, The

Andersons, Maumee, OH was provided for each mated female on

Day 18 of gestation. Fresh bedd ing was provided at least weekly

and as needed during the lactation period (litters were weaned on

Day 28 of lactation).

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2.9.8. BEDDING ANALYSIS

Analyses for each batch of bedding used on study can be found in

Appendix BB. There were no known contaminants in the bedding

that were expected to interfere with the results of this study.

2.9.9. ENVIRONMENTAL CONDITIONS

Light/Dark Cycle

A twelve

- hour light/dark cycle controlled by an automatic timer

was provided.

Temperature

Temperature was monitored in accordance with Testin g Facility

SOPs and maintained within the specified range to the maximum

extent possible. Excursions outside the specified range were

considered not to have affected the integrity of the study.

Desired: 19 to 25 ° C

Actual: 15.6 to 31.7 ° C

Average: 18.5 to 26.4 ° C

Relative Humidity

Relative humidity was monitored in accordance with Testing

Facility SOPs and maintained within the specified range to the

maximum extent possible. Excursions outside the specified range

were considered not to have affected the int egrity of the study.

Desired: 30 to 70%

Actual: 15.7 to 100.0%

Average: 22.0 to 75.5%

Air Changes

Air Changes were monitored in accordance with Testing Facility

SOPs and maintained within the specified range to the maximum

extent possible.

Desired: 10 - 15 per hour

Actual: 14.6 – 14.7 per hour

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2.10. TEST SUBSTANCE ADMINISTRATION AND CHAMBE R

OPERATION

Inhalation (whole-body exposures).

Details of test article administration and chamber operation information

are presented in the Inhalation Report (Appendix A).

2.11. EX PERIMENTAL EVALUATIONS

2.11.1. VIABILITY EXAMINATION (CAGESIDE)

Observations for mortality and signs of severe toxic or

pharmacological effects were made at least twice daily (morning

and afternoon).

2.11.2. DETAILED PHYSICAL EXAMINATIONS

The animals were observed as a group at least once during each

exposure.

In addition, each parental animal was removed from its cage and

examined twice pretest, and weekly thereafter during the study

period. Females were then continuously observed weekly until

there was evidence of mat ing. Once mated, females were observed

on Gestation Days 0, 7, 14, and 20 and on Lactation Days 1, 4, 7,

14, 21, and 28. Females that showed evidence of mating but did

not deliver were observed on Gestation Days 0, 4, 7, 14, and 20

and were euthanized on Gestation Day 25. Females without

evidence of mating were continuously observed weekly until

euthanized on the last possible Gestation Day 25. Examinations

included, but were not limited to, observations of general

condition, skin and fur, eyes, nose, oral cavity, abdomen and

external genitalia, as well as, evaluations of respiration and

palpation for tissue masses. During the exposure period, physical

examinations were performed post

- exposure except they were

performed pre -exposure during the gestation and lactation periods.

2.11.3. BODY WEIGHT

Each parental animal was removed from its cage and weighed

twice pretest, and weekly thereafter during the study period

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(including the mating period). Once mated, females were weighed

on Gestation Days 0, 4, 7, 14, and 20 and on Lactation Days 1, 4, 7,

14, 21, and 28. Females that did not show evidence of mating

and/or did not deliver pups were weighed weekly until euthanasia.

2.11.4. FEED CONSUMPTION

Feed consumption was measured once pretest and weekly

thereafter during the study period. Once mated, female feed

consumption was measured on Gestation Days 0, 4, 7, 14, and 20

and on Lactation Days 1, 4, 7, 14, 21, and 28 until euthanasia.

Females without evidence of mating and/or that did not deliver

pups had consumption measur ed weekly until euthanasia. Feed

consumption was not measured for any animal during the mating

period when males and females were co -housed.

Calculation

Feed Consumption (g/kg/day) =

grams of food consumed ÷ # days

body weight (kg) a

a 

## The average of the current and previous weight was used.



# 2.12. MATING, GESTATION AND LACTATION PROCEDUR ES

2.12.1. ESTROUS CYCLING

Daily vaginal smears were taken at approximately the same time

each day and the stage of the estrous cycle was determined for each

P 0 

# female beginning on Test Day 49

, three weeks prior to

cohabitation, and continued until there was evidence of mating or

until the 14-day mating period ended on Test Day 83.

2.12.2. MATING PROCEDURE

Following a 10

- week pre

- mating treatment period, one male and

one female from the same exposure group were caged together

until evidence of mating was observed (a copulation plug in the

vagina and/or microscopic observation of sperm in the vaginal

smear) or 14 consecutive days had elapsed. During cohabitation

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period exposures, males and f emales were not paired together. The

day evidence of mating was observed was defined as Day 0 of

gestation.

2.12.3. PATURITION AND LACTA TION

On Day 18 of gestation, several days prior to expected parturition,

each female was transferred to a solid plastic “shoe

- box” cage.

Bedding substance was provided and changed at least weekly as

needed. Examinations for signs of parturition were made twice

daily (morning and afternoon). The day on which parturition was

first observed was defined as Day 0 of lactation. Fe males that

exhibited no evidence of mating were transferred to plastic “shoe

box” cages when the first animals mated reached their Day 18 of

gestation.

2.12.4. LITTER EVALUATIONS ( F 1 

# )

Observations

F 1 

# litters were observed as soon as possible after delivery for the

number of live and dead pups, sex of the pups and pup

abnormalities. The sex was determined on the day of delivery

completion for all pups by inspection of the anogenital distance.

All pups were uniquely identified within the litter by toe tattoo on

the day of delivery completion. Thereafter, litters were observed

twice daily (morning and afternoon) for the presence of dead pups.

These dead pups were examined to the extent possible for defects

and/or cause of death ( lung floatation test to distinguish stillborn

and found dead pups) and preserved in neutral, phosphate

- buffered

10% formalin. Autolyzed remains found on the day of parturition

were presumed to be stillborn. Litter size was recorded daily from

Lactation Day 1 until weaning on Lactation Day 28.

Culling Pups

On Day 4 of lactation, each litter with more that 10 pups was

culled to that number, with sex distribution equalized (five/sex)

when possible. The pups were culled randomly, preferential

culling of runts not being performed.

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Physical Examinations

Each F 1 

# pup was given a macroscopic physical examination on the

day of delivery completion and on Day 4, 7, 14, 21 and 28 of

lactation.

Body Weight

Individual F 1 

# pup weights were recorded on Day 1, 4 (precull

intervals), 7, 14, 21 and 28 of lactation (postcull intervals).

Sexing

The sex of each individual pup was recorded on the day of delivery

completion, then reconfirmed on Lactation Day 4, 7, 14, 21, and at

weaning on Lactation Day 28.

2.13. POSTMORTEM

2.13.1. PARENTAL ANIMALS (P 0 

# )

Necropsy Information

Necro psy was performed on up to 26 animals/sex/group. All P 0 

# males were euthanized after the last day that the F 1 

# litters were

delivered. All P 0 

# females with litters were euthanized on Day 28

of lactation. P 0 

# females with no confirmed day of mating were

eutha nized on last possible Gestation Day 25. P 0 

# females with

confirmed days of mating that did not deliver were euthanized on

Gestation Day 25. Animals were fasted overnight prior to

necropsy.

If a dam entirely lost her litter, she resumed exposures the nex t day

(if prior to lactation day 5) or otherwise continued exposures. She

was sacrificed on the initial day of weaning for the study (or later,

as practical) and macroscopically examined and tissues retained

but no organs were weighed.

Method of Euthanasi a

Exsanguination following anesthesia with inhaled carbon dioxide.

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Macroscopic Examination

Complete macroscopic postmortem examinations were performed

on all adult animals, and all abnormal observations were recorded.

The necropsy of the parental animals included examination of the

external surface and all orifices; the external surfaces of the brain

and spinal cord; the organs and tissues of the cranial, thoracic,

abdominal and pelvic cavities and neck; and the remainder of the

carcass. Examination of a ll P 0 

# females included a vaginal smear to

determine the stage of the estrous cycle and a count of uterine

implantation scars, if present.

Organ Weights

Organs indicated in Table I (page 25 ) were weighed for all animals

at the sche duled sacrifice intervals. Prior to weighing, the organs

were carefully dissected and properly trimmed to remove adipose

and other contiguous tissues in a uniform manner. Organs were

weighed as soon as possible after dissection in order to avoid

drying. Paired organs were weighed together (gonads were

weighed separately). Organ weight data is presented as absolute

values and relative to terminal body weight and brain weight.

Organ weights were not recorded for an animal dying

spontaneously or euthanized moribund.

Tissues Preserved and Examined Histopathologically

The tissues listed in Table I (page 25) were obtained at the

scheduled sacrifice intervals and preserved for all animals. Slides

of the indicated tissues were prepared and examined

microscopica lly for 10 randomly selected animals per sex per

group in the con trol and high exposure groups. Any abnormalities

not noted during macroscopic examinations which were seen

during histology processing were recorded.

Histopathological examinations of the te stes were conducted to

identify potential treatment

- related effects such as retained

spermatids, missing germ cell layers or types, multinucleated giant

cells, or sloughing of spermatogenic cells into the lumen. The

examination of the intact epididymis wa s of a longitudinal section

through the caput, corpus and cauda regions to permit detection of

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lesions such as sperm granulomas, leukocytic infiltration

(inflammation), aberrant cell types within the lumen, or the

absence of clear cells in the cauda epidid ymal epithelium.

Histopathological examination of the ovary included evaluation of

five sections taken at least 100 μ m apart from the inner third of

each ovary permitting detection of depletion of the primordial

follicle population and enumeration of the total number of

primordial follicles for comparison with the ovaries from control

animals. These examinations also confirmed the presence or

absence of growing follicles and corpora lutea in comparison to

control ovaries.

TABLE I

O RGAN NAME WEIGHED PRESERVED EXAMINED

MICROSCOPICALLY

(Groups I and IV)

adrenal glands X X X

brain (medulla, pons, cerebrum and

cerebellum) X X

epididymides (total and caudal) X X a 

### X a



### kidneys X X

liver X X

lungs (with mainstem bronchi) X X X

ovari es X X X

pituitary gland X X X

prostate gland X X X

seminal vesicles with coagulating glands X X X

spleen X X

testes X X a 

### X a



### uterus (body/horns) with cervix and oviducts X X X

vagina X X

tissues with macroscopic findings including

tissue masses X X

a 

### Right testis and epididymis only.



# Preservatives

All tissues

- 10% neutral buffered formalin (NBF).

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Testes and epididymides were placed in Modified Davidson’s

solution initially and then retained in 10% formalin. Lungs were

infused with formalin pr ior to their immersion into a larger volume

of the same fixative.

Processing

After fixation, the tissues and organs from all animals were

routinely processed, embedded in paraffin, cut at a microtome

setting of 4 -7 microns, mounted on glass slides and stained with

hematoxylin and eosin.

2.13.2. F 1 

# PUPS

Method of Euthanasia

Culled F 1 

# pups, or those sacrificed prior to the day of weaning,

were euthanized by IP injection of sodium pentobarbital.

W eanling s were euthanized by exsanguination after anesthesia with

carbo n dioxide.

Dead and Culled Pups

F 1 

# pups found dead at birth were identified (lung floatation test) as

stillborn or live

- born but found dead. F 1 

# pups found dead during

the lactation period were examined to the maximum extent

possible for defects and/or th e cause of death, and for the presence

or absence of milk in the stomach. Dead pups were preserved

intact in 10% NBF. Partially cannibalized pups were examined to

the maximum extent possible and discarded or preserved intact in

10% NBF. Culled F 1 

# pups w ere examined for external

abnormalities. If unremarkable, these pups were then euthanized

and discarded. Culled F 1 

# pups with external abnormalities were

preserved intact in 10% NBF.

Macroscopic Examination and Tissues Preserved

Macroscopic examinations were performed on up to 3 randomly

selected pups/sex/litter on Day 28 of lactation. Examinations

included observations of any structural abnormalities or

pathological changes. Special attention was given to the organs of

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the reproductive system, brain, liver, kidneys, pituitary, adrenal

gland, spleen, heart and thymus gland.

All remaining F 1 

# pups were examined for external abnormalities

and euthanized. Pups with external abnormalities were preserved

intact in 10% NBF. Pups with no external abnormalities were

discarded. No further macroscopic postmortem examinations were

performed.

Organ Weights

The following organs were weighed from one randomly selected

pup/sex/litter sacrificed on Day 28 of lactation:

brain

spleen

thymus gland

Organ weight data is pr esented as absolute values and relative to

terminal body weight and brain weight.

2.14. SPERM COUNT, MOTILITY AND MORPHOLOGY

ASSESSMENTS

The following sperm evaluations were conducted for all control and high

exposure P 0 

# males (samples and images were collected but not evaluated

for all other males): 1) motility; 2) a count of homogenization

- resistant

testicular spermatids; 3) a count of caudal epididymal sperm; and 4) sperm

morphology (cauda epididymis). Evaluations were performed by

Pathology Associates International, Frederick, MD (PAI)as follows:

The right testis and right epididymis from each animal were removed

intact, weighed (testes weighed together and also separately) and

preserved.

The left epididymis was removed intact, weighed, and frozen on dry ice

for transport to PAI. The epididymides were stored frozen at -70 ° C until

evaluation for caudal sperm count. Each epididymis was thawed and the

caudal portion removed and weighed. A homogenized sample of the

caudal epididymis was stained and then examine d using the Hamilton

Thorne IVOS sperm analyzer. For each stained preparation, 20 fields were

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counted. The total number of sperm in the caudal epididymis was

calculated and adjusted for the caudal epididymal weight. Additionally,

for each male, two sper m morphology slides were prepared, stained with

Eosin and evaluated for morphological development.

The left vas deferens was excised and placed in a prewarmed solution of

phosphate buffered saline and 1% Bovine Serum Albumin. After a

minimum three minute “swimout” period, a sample was placed in a

Hamilton Thorne IVOS sperm analyzer. Five fields were selected and

stored as digital images. The images were analyzed for percent motility

and transferred to optical media for permanent storage.

The left testis w as removed and frozen on dry ice for transport to PAI.

The testes were stored frozen at -20 ° C until processed for counting of

homogenization- resistant spermatids.

(See Appendix AA for details and methodology.)

Tissues retained from these evaluations will be discarded following

issuance of the final report following consultation with the Sponsor.

2.15. STATISTICAL ANALYSIS

2.15.1. CONTINUOUS DATA

The following parameters were analyzed statistically:

Body weights

Body weight change

Feed consumption values

Organ weight d ata

Estrous cycle data

Gestation length

Pup body weights

Number of pups (live, dead, total)

Method of Analysis

Mean values of all exposure groups were compared to the mean

value for the control group at each time interval.

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For all parameters, the stan dard one

- way analysis of variance

(ANOVA) using the F ratio to assess significance was used

(Dunlap and Duffy, 1975). If significant differences among the

means were indicated, additional testing was performed using

Dunnet’s t

- test to determine which means were significantly

different from the control (Dunlap et al., 1981). All t

- tests were

conducted at the 5% and 1% significance levels.

Exceptions

Statistical evaluations were not performed when the standard

deviation for the control group was 0 and/or N (number of animals)

in the control group was less than or equal to two.

Dose groups were eliminated from statistical analysis if their

standard deviation was 0 and/or N (number of animals) in the

group was less than or equal to two.

2.15.2. SPERM AND MOTILITY ANALYSIS

The following parameters were analyzed statistically:

Mean sperm count (homogenization

- resistant testicular spermatid

count and caudal epididymal sperm count) and motility data

.



# A Kruskal

- Wallis nonparametric ANOVA test was performed to

assess signif icance (Kruskal and Wallis, 1952, 1953). If a

significant difference occurred (p < 0.05), the Wilcoxon (Mann

Whitney U) test was used for pair

- wise comparisons of each

treated group to the vehicle control group. Statistics were

performed using an IBM compa tible computer with SAS computer

programs (SAS/STAT User’s Guide, 1989).

2.15.3. INCIDENCE DATA

The following parameters were analyzed statistically:

Mortality rates

Mating indices (male and female)

Pregnancy rates

Male fertility indices

Live birth indices

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Pup via bility indices (Days 0

- 4) and lactation indices (Days 4 -28)

Incidence Data Analysis

A Fisher Exact Test with Bonferonni correction was performed to

identify differences between the groups (Seigel, 1956).

2.16. DATA STORAGE

All raw data, preserved specimens, and retained samples, as well as the

original study protocol and the original final report are to be maintained in

the Archives of the Testing Facility upon completion of the study. The

Sponsor will determine the final disposition of these materials.

All raw data and all reports generated by Pathology Associates

International (PAI), Frederick, MD were maintained by PAI. After

submission of the final report, all of the above will be shipped to

Huntingdon Life Sciences to be archived.

2.17. REGULATORY REFERENCE S

2.17.1. TEST GUIDELINE

This study was designed to meet or exceed the pertinent

requirements of:

US EPA Vehicle Emissions Inhalation Exposure Guideline 79.61,

CFR Vol. 59, No. 122, 27 June 1994.

US EPA (Environmental Protection Agency) Health Effects Test

Guidelines, OPPTS 870.3800, Reproduction and Fertility Effects

(August 1998) except this study will only continue through the 1 st



# generation of offspring.

2.17.2. GOOD LABORATORY PRAC TICES

This study was conducted in accordance with US EPA 79.60, CFR

Vol. 59, No. 122, 27 June 1994 and was performed according to

the protocol and Huntingdon Life Sciences ’ and Pathology

Associates International’s Standard Operating Procedures.

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2.17.3. ANIMAL WELFARE ACT COMPLIANCE

This study complied with all appropriate parts of the Animal

Welfare Act re gulations: 9 CFR Parts 1 and 2 Final Rules, Federal

Register, Volume 54, No. 168, August 31, 1989, pp. 36112 -36163

effective October 30, 1989 and 9 CFR Part 3 Animal Welfare

Standards; Final Rule, Federal Register, Volume 56, No. 32,

February 15, 1991, pp. 6426-6505 effective March 18, 1991.

2.18. PROTOCOL DEVIATIONS

The following protocol deviations occurred during the study but were not

considered to have compromised the validity or integrity of the study:

1. Due to supplier error, Charles River Laboratories did not supply male

and female animals from different rooms. Therefore, the mating of

male and female littermates, prohibited by protocol, may have

occurred.

2. Due to technician error, Group II female Animal No. 2543 was not co

housed with a male on 30 July 2002, the mating initiation date. The

animal was co -housed on 31 July 2002.

3. Due to technician error, Group II female Animal No. 2542 was found

pregnant on 30 July 2002 but was not separated from the male until

31 July 2002. As a result, feed consumption wa s not initiated until

31 July 2002 which was Gestation Day 1.

4. Due to technician error, Group IV female Animal No. 4542 was

inadvertently loaded into the exposure chamber and exposed on

Gestation Day 20.

5. Due to technician error, Group I animals were unloade d from the

exposure chamber prior to completion of the 30 minute clearing period

on 25 August 2002.

6. Due to technician error, the disposition of the extra animals not used

on study was not recorded in the Study File. The animals would have

been euthanized or transferred to the in -house colony.

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7. Due to equipment malfunction, sperm motility images were not

captured for Group I Animal No. 1026 and Group IV Animal

No. 4026.

8. Although not required, a lung floatation test was performed for Pup

No. 3534-1 which was found dead on Lactation Day 1.

9. Due to technician error, t here is no documentation that the spleen was

collected for Animal Nos. 1039, 1041, 1043, 1044 and 1049 and no

saved tissues could be located for these animals.

10. Weanling pups from Dam No. 4538 (pups 2 through 11) were

euthanized via an intraperitoneal injection of sodium pentobarbital

instead of by exsanguination after anesthesia with carbon dioxide.

11. Although partially cannibalized pups were to be discarded

, tissues

were saved for Pup No. 2538-4 , foun d dead and partially cannibalized

, on Lactation Day 1.

12. Due to technician error, l eft testis specimens were stored at -70°C prior

to processing instead of -20°C.

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3. RESULTS AND DISCUSSI ON

3.1. CHAMBER MONITORING

(Appendices A and B)

Chamber distribution analyses (see Table IV in Appendix A) showed that

the test substance was evenly distributed within each chamber. Chamber

monitoring (see Table VI in Appendix A) showed tha t the chamber oxygen

levels were 20%. Chamber room monitoring (see Table VI in

Appendix A) showed that no test substance was present in the room and

that the sound and light levels were acceptable.

The target and mean ( ± standard deviation) analytical (IR ) and nominal

concentrations are summarized as follows:

Group Test

Substance Target

Concentration

( mg/m 3 

# ) Analytical

Concentration

(mg/m 3 

# ) Nominal

Concentration

( mg/m 3 

# )

I Air Control 0 0 ± 0 0 ± 0

II Gasoline DIPE

Vapor

Condensate 2000 2034 ± 128 2086 ± 116

III Gasoline DIPE

Vapor

Condensate 10000 10260 ± 621 1023 1 ± 422

IV Gasoline DIPE

Vapor

Condensate 20000 20230 ± 775 1990 3 ± 635

The analytically measured exposure levels of the airborne test substance

were acceptably close , in the opinion of the study director, to the targeted

exposure levels. The measured and nominal concentrations varied

somewhat (less than 3%), but acceptably, from the expected 1:1 ratio for

this type of vapor exposure. Chamber environmental conditions averaged

25oC temperature and 46% relative humidity.

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Mean particle size distribution measurements for the exposures are

summarized as follows:

Group Test

Substance Mass Median

Aerodynamic

Diameter

(μm) Geometric

Standard

Deviation Total

Mass

Concentration

(mg/m 3 

# )

I Air Co ntrol 2.478 2.117 6.31 x 10 -3



# II Gasoline DIPE

Vapor

Condensate 1.930 2.074 6.28 x 10 -3



# III Gasoline DIPE

Vapor

Condensate 1.974 2.281 7.75 x 10 -3



# IV Gasoline DIPE

Vapor

Condensate 2.142 2.182 7.83 x 10 -3



# These results indicated that the atmospheres we re essentially vapor only,

as expected, since there was no substantial difference between the test

substance chambers and the Air Control chamber.

Analysis of the major components in the neat test substance and the test

atmospheres (see Appendix B) showed an acceptably close comparison

between the neat test substance and the vaporized test substance. This

data demonstrated that the test animals were exposed, as expected, to all of

the major components of the test substance in their acceptably proper

propor tion. The data was consistent from week

- to

- week during the study

indicating stability of the test substance and the atmosphere generation

techniques.

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3.2. PARENTAL DATA (P 0 

# GENERATION)

3.2.1. MORTALITY

(Table s 1, 3 and 4; Appendices C, E and F)

There was no effect of treatment on survival. One male rat

(#3043) in the 10000 mg/m 3 

# group was humanely sacrificed on

Day 23 due to an injury to the animal’s snout. Another male rat

(#3044) in the 10000 mg/m 3 

# group was found dead on Day 110

with no prior clinical signs or bo dy weight effects. However, the

gross necropsy examination found an extremely enlarged spleen

and liver and discolored thymus.

One female rat (#2536) in the 2000 mg/m 3 

# group was humanely

sacrificed on Day 78 (during the gestation phase) due to an injury

t o the animal’s teeth. Another female rat (#4538) in the

20000 mg/m 3 

# group was humanely sacrificed on Day 106 (during

the lactation phase) due to an injury to the animal’s snout.

3.2.2. PHYSICAL OBSERVATION DATA

(Tables 2, 3 and 4; Appendices A, D, E and F)

The test animals were unremarkable in

- chamber during the

exposure periods.

The test animals were generally unremarkable during the non

exposure periods (afternoon evaluations) during the premating

period in both sexes, the mating/postmating period in the male r ats,

and the gestation and lactation periods in the female rats. Scattered

observations such as chromodacryorrhea and lacrimation were

noted but were not treatment- related.

3.2.3. BODY WEIGHTS

– PREMATING PERIOD

(Tables 5 and 6; Appendices G and H)

There were no exposure- related differences in body weights or

weight changes in the test substance exposed animals compared to

the Air Control animals. While a few statistically significant

differences in body weight gain were seen at several intervals in

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the test sub stance exposed animals, these were not in a treatment

related pattern.

3.2.4. MATERNAL BODY WEIGHT S

- GESTATION AND

LACTATION PERIODS

(Tables 7, 8, 9 and 10; Appendices I, J, K and L)

There were no exposure

- related differences in body weights or

weight changes in the test substance exposed animals compared to

the Air Control animals. The slightly greater weight gain during

gestation, well within normal variation, is most likely due to the

slightly larger litter sizes in the exposed groups. While a

statistically s ignificant decrease in weight gain was noted for the

mid

- level exposed group from days 21 to 28 during the lactation

period, this was not in a treatment

- related pattern. The variations

in gain and loss patterns among the groups during lactation suggest

th at these weight changes are natural fluctuations in lactating

animals and not linked specifically to exposure.

3.2.5. FEED CONSUMPTION

– PREMATING PERIOD

(Table 11; Appendix M)

There were no exposure

- related differences in feed consumption in

the test substance e xposed animals compared to the Air Control

animals. While a few statistically significant differences were

noted at various intervals, the absolute differences were generally

small and were not in a treatment

- related pattern.

3.2.6. MATERNAL FEED CONSUM PTION - G ESTATION AND

LACTATION PERIODS

(Tables 12 and 13; Appendices N and O)

There were no exposure

- related differences in feed consumption

during the gestation and lactation periods in the test substance

exposed animals compared to the Air Control animals. Whil e

statistically significant increases were noted at two intervals for the

mid -level exposed group during the lactation period, these were not

considered as adverse responses because modestly increased feed

consumption (as opposed to decreased feed consumpt ion)

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especially in the absence of a dose response pattern is not identified

as a potentially toxic effect.

3.2.7. ESTROUS CYCLE DATA

– PREMATING PERIOD

(Table 14; Appendix P)

There were no exposure

- related differences in estrous cycle data

(as measured by cycle l ength and number of estrous cycles) in the

test substance exposed animals compared to the Air Control

animals. One female in each of the Control and 10000 mg/m3

exposed groups showed signs of having entered pseudopregnancy,

with prolonged diestrus after the first or second estrus while paired

with a male, and did not produce a litter. One female in each of the

2000 and 20000 mg/m3 exposed groups also showed this pattern,

but they were found pregnant and it was concluded that the mating

evidence at the fir st estrus while paired with the male had not been

observed. One female in the 20000 mg/m3 exposed group showed

an abnormal cycle (irregular length and then persistent estrus) and

did not become pregnant. These occurrences were considered

incidental.

3.2.8. MATI NG, FERTILITY AND GESTATION INDICES

(Tables 15 and 16; Appendices Q and R)

Mating indices for the male rats treated with the test substance

were comparable to the Air Control group. Mating, fertility and

gestation indices for the female rats treated with the test substance

were comparable to the Air Control group and normal for this

strain of animal. Most of the females in each group mated at the

first opportunity (first estrus after initial pairing) with only

occasional animals mating at the second estrus. There were also no

treatment

- related differences in the other reproductive parameters

up to the time of parturition including the percent of females

completing delivery and the duration of gestation, when compared

to the Air Control group.

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3.2.9. PARTURITION AND LITTER SURVIVAL DATA

(Table 16; Appendices R and S)

There were no treatment related inter

- group differences among the

parturition and pup survival parameters. The number of

implantation sites per dam, the number of pups delivered (live or

dead), subse quent viability (to Day 4 and then to Day 21 post

partum) and sex ratio were normal for this strain of animal.

Statistically significant differences between treated groups and the

Air Control group were a result of the incidental finding that the

Air Control group lost more pups due to a total litter loss in one

dam prior to Day 4 post partum.

3.3. PUP DATA (F 1 

# GENERATION)

3.3.1. OBSERVATION DATA

(Table 17; Appendix T)

The pups were unremarkable during the lactation period. Scattered

observations were noted but were not treatment

- related.

3.3.2. BODY WEIGHT DATA

(Tables 16 and 18; Appendix U)

There was a slight, uniform reduction in pup body weight

performance over the first 4 days after birth across the groups

exposed to the test substance, as compared with Air Control. T his

was commensurate with the slightly larger live litter size in these

groups, as compared with Air Control, and was considered not to

represent an adverse effect of treatment. Following the

‘standardization’ of live litter size (via culling) at Day 4 af ter birth,

pup body weight performance became uniform across all the

groups.

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3.4. POSTMORTEM DATA

3.4.1. TERMINAL ESTROUS AND MACROSCOPIC

POSTMORTEM EVALUATIO NS

- PARENTAL

GENERATION

(Table 19; Appendices V and Z)

There were no exposure

- related differences in terminal estrous

evaluations in the test substance exposed animals compared to the

Air Control animals. Scattered macroscopic postmortem

observations such as lung discoloration were noted in a treatment

related pattern, but in absence of any effects on lung weigh ts or

histopathology (see following sections), these findings were not

considered adver se

.

3.4.2. ORGAN WEIGHTS

- PARENTAL GENERATION

(Table 21; Appendix X)

Exposure- related effects on organ weights included statistically

significant increases in kidney weights ( absolute and/or relative to

body and brain weight) at the 2 higher exposure levels in the males.

These differences for the males were consistent with the

hydrocarbon nephropathy findings at the 20000 mg/m 3 

# exposure

level in the 13

- week inhalation study 00

- 6130 with this same test

substance. However, these same findings were not seen

microscopically with these test animals. Also, this finding has

been generally accepted (US EPA 1991. Alpha 2 microglobulin:

association with chemically induced renal toxicit y and neoplasia in

male rat. In Risk Assessment Forum, p.85. US Govt. Printing

Office, Washington, DC.) not to be relevant to human risk

assessment.

Exposure- related effects on organ weights also included

statistically significant increases in liver weigh ts (absolute and/or

relative to body weight or brain weight) at the 2 higher exposure

levels in the males. These may have been the result of enzyme

induction by the test substance but were not correlated with any

histopathology finding (see following section).

# Huntingdon Life Sciences 00-4205 Page 40

211- DIPE -1G Final Report

A few other statistically significant differences from Air Control

were apparent. The differences in testis weight at 20000 mg/m3

(relative to brain weight only) and epididymis weight at 10000 and

20000 mg/m3 were of a very small magnitude (in the region of

5- 8%) and were discounted as being of no biological significance.

Equally, the difference in seminal vesicle/coagulating gland weight

(approximately 17% higher in the 20000 mg/m3 group) was not



# considered to be of definitive toxicological signifi cance

, considering also that neither the histopathological examinations

nor the sperm evaluations revealed any evidence of treatment

related change in any of these organs (see relevant sections 3.4.3

and 3.4.6).

3.4.3. SPERM EVALUATIONS

– PARENTAL GENERATION

(App endix AA)

Mean percent motility, epididymal sperm count, homogenization

resistant testicular spermatid count, sperm morphology and caudal

epididymis weight were not affected by treatment at an exposure

level of 20,000 mg/m 3 

# . No biologically meaningful dif ferences were

observed between the study groups.

3.4.4. MACROSCOPIC POSTMORTEM EVALUATIONS

- PUPS

(Table 20; Appendix W)

There were no exposure

- related differences in macroscopic

postmortem evaluations in the pups feeding from test substance

exposed animals comp ared to the pups feeding from Air Control

animals. Scattered observations such as dilated renal pelvis were

noted but were not treatment- related.

3.4.5. ORGAN WEIGHTS

- PUPS

(Table 22; Appendix Y)

There were no exposure

- related differences in organ weights in th e

pups feeding from test substance exposed animals compared to the

pups feeding from Air Control animals. A few statistically

significant differences in thymus weights (relative to body weight)

were noted for the low

- and mid

- exposure groups. However, th ese

# Huntingdon Life Sciences 00-4205 Page 41

211- DIPE -1G Final Report

were not in a treatment

- related pattern and were comparable to

historical control data (see Appendix DD).

3.4.6. HISTOPATHOLOGICAL EV ALUATIONS

- PARENTAL

GENERATION

(Table 23; Appendix Z)

No test substance related microscopic changes were noted in male

and female reproductive organs or other protocol

- specified tissues

in this study. The remaining microscopic findings were similar to

those routinely observed in animals of this age and species and

were considered incidental.

4. CONCLUSION

Exposure of rats to 2000, 10000 and 20000 mg/m 3 

# of vapor of Gasoline DIPE

Vapor Condensate resulted in no effects on the exposed parental animals prior to

their termination. However, increases in liver weights in parental male animals

exposed to 10000 and 20000 mg/m 3 

# of vapor we re seen. Increases in kidney

weights in parental male animals exposed to the two higher exposure levels of

vapor were consistent with hydrocarbon nephropathy seen in similar animals

exposed for 13 weeks to this test substance. However, this finding has b een

generally accepted (US EPA 1991. Alpha 2 microglobulin: association with

chemically induced renal toxicity and neoplasia in male rat. In Risk Assessment

Forum, p.85. US Govt. Printing Office, Washington, DC.) not to be relevant to

human risk assessmen t. Therefore, a no observable effect level (NOEL) of

2000 mg/m 3 

# was determined. There was no effect at any of the exposure levels on

reproductive performance in the study, including mating, fertility, parturition,

lactation and offspring survival and dev elopment for 28 days after birth.

Therefore, with respect to reproductive performance, a no observable effect level

( NOEL) of 20000 mg/m 3 

# was determined

.

# Huntingdon Life Sciences 00-4205 Page 42

211- DIPE -1G Final Report

REFERENCES

Dunlap, W.P. and Duffy, J.A. 1975. Fortran IV functions for calculating exact

probabilities associated with z, chi-square, t and f values. Behav. Res. Methods and

Instrumentations 7: 59-60.

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV functions for calculating

probabilities associated with Dunnett’s test. Behav. Res. Methods and Instrumentation

13: 363-366.

Dunnett, C.W. 1955. A multiple comparison procedure for comparing several treatments

with a control. Journal of the American Statistica l Association 50: 1096-1121.

Dunnett, C.W. 1964. New tables for multiple comparisons with a control. Biometrics 20-

3: 482-491.

Games, P.A. and Howell, J.F. 1976. Pairwise multiple comparison procedures with

unequal n’s and/or variances: a monte- carlo s tudy. Journal of Educational Statistics 1:

113-125.

Kruskal, W.H. and Wallis, W.A. 1952. Use of Ranks in One- Criterion Variance Analysis.

Journal of the American Statistical Association 47: 583-621.

Kruskal, W.H. and Wallis, W.A. 1953. Errata for Krusk al -Wallis (1952). Journal of the

American Statistical Association 48: 907-911.

Mantel, N. 1963. Chi-square test with one degree of freedom: extensions of the MantelHaenszel procedure. Journal of the American Statistical Association 58: 690-700.

Seige l, S. 1956. Nonparametric Statistics for the Behavioral Sciences. McGraw- Hill.

# Huntingdon Life Sciences 00

- 4205 Page 43

211

- DIPE

- 1G Final Report

Calculations

Female Mating Index:

no. of females with confirmed mating (sperm and/or vaginal plug) plus no. of

pregnant females without evidence of mating (no sperm or vaginal plug)/no. of

females placed with males

Female Fertility Index:

no. of females pregnant/no. of females confirmed mating or pregnancy for

females without evidence of mating

Male Mating Index:

no. of males with confirmed mating with a female or pregnancy for females

without evidence of mating/no. of males placed with females

Male Fertility Ind ex:

no. of males mating and impregnating a female plus the no. of males with a

pregnant female without evidence of mating/no. of males with confirmed mating

plus no. of males with a pregnant female without evidence of mating

Gestation Index:

no. of females with liveborn/no. of females with confirmed pregnancy

Viability Index:

no. of pups alive Day 4 precull/no. of liveborn pups

Lactation Index:

no. of pups alive Day 21/no. of pups Day 4 postcull

Live Birth Index:

total no. of liveborn pups/total no. of pups born

# Huntingdon Life Sciences 00

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- 1G Final Report

General Preface

General Notes

Individual animal data values presented in this report may be rounded.

Unrounded individual animal data values are used to calculate the reported

mean and standard deviation values. Therefore, use of the reported individual

values to reproduce means, standard deviations and/or to perform any

subsequent calculations may produce minor discrepancies between the

calculated values and those presented in this report.

Key to Abbreviations

M = Male

F = Female

No = Number

Gen. = General

PG = Pregnant

Group Group

Designation Exposure

Levels

(mg/m 3 

# )

I Control 0 (air only)

II Low 2,000

III Middle 10,000

IV High 20,00011 -JAN- 2010 11: 46

Huntingdon Life Sciences 00

- 4205 PAGE 45

TABLE 1 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF SURVIVAL AND PREGNANCY

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

No. of males at start N 26 26 26 26

Premating

- Died/sacrificed N 0 0 1 0

Postmating

- Died/sacrificed N 0 0 1 0

No. of females at start N 26 26 26 26

No. of females cohabitated N 26 26 26 26

- Without evidence of mating N 1 3 2 4

Pregnant N 0 1 0 1

Nonpregnant N 1 2 2 3

Pregnant N 22 24 24 23

- Died/sacrificed N 0 1 0 0

- Died delivering N 0 0 0 0

- Died/sacrificed post partum N 0 0 0 1

- Aborted died/sacrificed N 0 0 0 0

Nonpregnant N 4 2 2 3

- Died/sacrificed N 0 0 0 0

Total females died/sacrificed N 0 1 0 1

% 0.0 4.3 0.0 4.5

Dams delivering N 22 23 24 23

- With liveborn pups N 22 23 24 23

% 100.0 100.0 100.0 100.0

- With all pups stillborn N 0 0 0 0

% 0.0 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences

# Huntingdon Life Scien ces 00

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211

- DIPE

- 1G Final Report

Summary of Clinical Observations

Preface Table 2

Number of animals examined represents the total number of animals observed

and animals, which were found dead, died accidentally or were killed in a

moribund condition or at a scheduled sacrifice for a given interval. Deaths

occurring between scheduled observation intervals are presented at the next

scheduled interval.

For summarization purposes, descriptive comments [i.e., location of scab(s)

and/or sore(s), etc.] are not presented in this table. These data are contained in

the study raw data if needed.

Total represents a cumulative total of all animals with the indicated observation

one or more times during the study.

Corresponding exposure levels for each group were as follows:

Group I

- 0 mg/m 3



# Group II

- 2,000 mg/m 3



# Group III

- 10,000 mg/ m 3



# Group IV

- 20,000 mg/m 3



# Generation Intervals:

P 0 

# Animals

Premating: Days

– 7 to 69

Mating: Days 70 to 83

Postmating: Day s 84 to 11211

- JAN

- 2010 11:48

Huntingdon Life Sciences 00

- 4205 PAGE 47

TABLE 2 P0 GENERATION

GASOLINE DIPE VA POR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES SUMMARY OF CLINICAL OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

GROUP#

- 7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

III 26 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25

IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

Normal

WITHIN NORMAL LIMITS I 26 26 25 25 25 25 24 24 23 22 22 21 21 21 21 20 19 20 26

II 26 26 26 26 26 26 26 24 24 24 24 26 24 24 25 25 25 24 26

III 26 26 26 26 22 21 22 22 21 20 20 19 19 20 20 21 21 20 26

IV 26 26 26 24 24 24 24 23 22 22 21 20 20 20 20 21 21 21 26

Dead

HUMANE SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

FOUND DEAD I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Gen. Appearance

SWOLLEN DIGIT I 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

SWOLLEN PAW(S) I 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 011

- JAN

- 2010 11:48

Huntingdon Life Sciences 00

- 4205 PAGE 48

TABLE 2 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES SUMMARY OF CLINICAL OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

GROUP#

- 7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

III 26 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25

IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

Dermal

- General

ULCERATION

- CERVICAL I 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

ALOPECIA

- I 0 0 0 0 0 0 1 2 2 3 3 3 2 2 2 2 1 1 3

EXTREMITIES/SNOUT II 0 0 0 0 0 0 0 1 2 2 2 0 1 1 1 1 1 1 3

III 0 0 0 0 3 3 3 3 4 4 4 4 4 3 3 3 3 3 5

IV 0 0 0 1 1 1 1 2 2 2 3 3 3 2 2 2 1 1 4

SCABS I 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1

ALOPECIA

- GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

II I 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 1 1

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Ocular

CHROMODACRYOR RHEA

- I 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1

BILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1

IV 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 111

- JAN

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Huntingd on Life Sciences 00

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TAB LE 2 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES SUMMARY OF CLINICAL OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

GROUP#

- 7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

II 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

III 26 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 25

IV 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26

CHROMODACRYORRHEA

- I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 3

UNILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 2

IV 0 0 0 0 0 0 0 1 0 1 1 1 1 1 1 1 1 2 2

LACRIMATION

- UNILATERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1

Oral/Buccal

INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 0 1 0 1 1 1 1 1 2 2 2 3

II 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 1 3

III 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 2

IV 0 0 0 0 0 0 0 0 1 0 1 2 2 4 3 3 3 3 4

ORAL SORE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

INCISORS MALOCCLUDED I 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 1

IV 0 0 0 0 0 0 0 1 2 1 3 3 3 3 4 3 3 3 411

- JAN

- 2010 11:48

Huntingdon Life Sciences 00

- 4205 PAGE 50

TABLE 2 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES SUMMARY OF CLINICAL OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

GROUP#

- 7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 22 1 1 1 1 1 1

II 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 2 2 2

III 26 26 26 26 26 26 26 26 26 26 26 23 2 2 2 2 2 2

IV 26 26 26 26 26 26 26 26 26 26 26 23 4 4 4 3 3 3

Normal

WITHIN NORMAL LIMITS I 26 26 26 26 26 25 25 23 21 21 21 18 1 1 1 1 1 0 26

II 26 26 26 26 26 25 25 25 23 23 21 21 3 3 3 2 2 0 26

III 26 26 26 25 26 26 26 25 26 26 25 22 2 2 2 2 2 0 26

IV 26 26 26 26 26 25 25 25 24 25 24 21 4 4 4 3 3 0 26

Dead

TERMINAL SACRIFICE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3

Gen. Appearance

SWOLLEN DIGIT I 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1

II 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Dermal

- General

ALOPECIA

- I 0 0 0 0 0 0 1 2 3 3 3 3 0 0 0 0 0 0 3

EXTREMITIES/SNOUT II 0 0 0 0 0 0 1 1 3 3 4 2 0 0 0 0 0 0 4

III 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 2

IV 0 0 0 0 0 1 1 1 1 1 1 1 0 0 0 0 0 0 111

- JAN

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Huntingdon Life Sciences 00

- 4205 PAGE 51

TABLE 2 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES SUMMARY OF CLINICAL OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

GROUP#

- 7 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 112 TOTAL

-----------------------------------------------------------------------------------------------------------------------------------

# OF ANIMALS EXAMINED I 26 26 26 26 26 26 26 26 26 26 26 22 1 1 1 1 1 1

II 26 26 26 26 26 26 26 26 26 26 26 23 3 3 3 2 2 2

III 26 26 26 26 26 26 26 26 26 26 26 23 2 2 2 2 2 2

IV 26 26 26 26 26 26 26 26 26 26 26 23 4 4 4 3 3 3

ALOPECIA

- GENERAL I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1

IV 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1

Ocu lar

CHROMODACRYORRHEA

- I 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0 0 0 1

UNILATERAL II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

LACRIMATION

- UNILATERAL I 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 1

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Oral/Buccal

INCISORS BROKEN/MISSING I 0 0 0 0 0 0 0 1 2 1 1 1 0 0 0 0 0 0 2

II 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

III 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1

IV 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1

INCISORS MALO CCLUDED I 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1

II 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1

III 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

IV 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 111 -JAN- 2010 11: 52

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TABLE 3 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF CLINICAL OBSERVATIONS DURING GESTATION

- (frequency/animals)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 to 20

Normal

----------

WITHIN NORMAL LIMITS 79/ 20 69/18 91/23 80/20

HUMANE SACRIFICE 0/ 0 1/ 1 0/ 0 0/ 0

Gen. Appearance

-------------------

SWOLLEN DIGIT 2/ 1 0/ 0 0/ 0 0/ 0

MISSING DIGIT 2/ 1 0/ 0 0/ 0 0/ 0

Dermal

- General

------------------

ALOPECIA

- EXTREMITIES/SNOUT 13/ 4 16/ 5 5/ 2 4/ 1

ALOPECIA

- GENERAL 3/ 1 4/ 1 4/ 1 3/ 1

Ocular

----------

CHROMODACRYORRHEA

- BILATERAL 0/ 0 1/ 1 0/ 0 0/ 0

CHROMODACRYORRHEA

- UNILATERAL 4/ 1 0/ 0 0/ 0 0/ 0

Oral/Buccal

---------------

INCISORS BROKEN/MISSING 4/ 1 1/ 1 0/ 0 0/ 0

INCISORS MALOCCLUDED 0/ 0 0/ 0 0/ 0 4/ 1

Room Check

--------------

DECREASED FECAL VOLUME 0/ 0 1/ 1 0/ 0 0/ 012

- JAN

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TABLE 4 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

- (frequency/animals)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 1 to 30

Normal

----------

WITHIN NORMAL LIMITS 104/ 18 107/18 128/23 122/21

HUMANE SACRIFICE 0/ 0 0/ 0 0/ 0 1/ 1

TERMINAL SACRIFICE 22/22 23/23 24/24 22/22

Gen. Appearance

-------------------

EMACIATION 1/ 1 0/ 0 0/ 0 0/ 0

Dermal

- General

------------------

ALOPECIA

- EXTREMITIES/SNOUT 19/ 4 30/ 5 15/ 3 8/ 2

ALOPECIA

- GENERAL 6/ 1 6/ 1 4/ 1 6/ 1

YELLOW ANO

- GENITAL STAINING 1/ 1 0/ 0 0/ 0 0/ 0

Ocular

----------

CHROMODACRYORRHEA

- UNILATERAL 6/ 1 0/ 0 0/ 0 0/ 0

Oral/Buccal

---------------

INCISORS BROKEN/MISSING 6/ 1 1/ 1 0/ 0 0/ 0

INCISORS MALOCCLUDED 0/ 0 0/ 0 0/ 0 6/ 1

Room Check

--------------

INCISORS MALOCCLUDED 0/ 0 0/ 0 0/ 0 1/ 1

SWOLLEN SNOUT 0/ 0 0/ 0 0/ 0 1/ 1

RED EXUDATE SNOUT 0/ 0 0/ 0 0/ 0 1/ 111

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TABLE 5 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY

- 7 MEAN 145 145 145 145

S.D. 10.4 10.5 10.5 10.8

N 26 26 26 26

DAY 0 MEAN 213 212 213 213

S.D. 14.1 11.9 13.4 12.8

N 26 26 26 26

DAY 7 MEAN 267 265 269 266

S .D. 17.6 16.9 16.9 17.6

N 26 26 26 26

DAY 14 MEAN 319 315 321 317

S.D. 22.6 21.9 22.1 22.7

N 26 26 26 26

DAY 21 MEAN 360 357 356 358

S.D. 26.6 25.8 27.5 27.1

N 26 26 26 26

DAY 28 MEAN 389 389 388 387

S.D. 29.4 29.6 28.1 30.1

N 26 26 25 26

DAY 35 MEAN 419 420 418 415

S.D. 33.4 34.7 33.2 34.8

N 26 26 25 26

DAY 42 MEAN 440 446 440 440

S.D. 40.8 38.6 35.9 40.1

N 26 26 25 26

DAY 49 MEA N 462 465 459 459

S.D. 37.8 43.1 38.0 41.5

N 26 26 25 26

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 11:54

Huntingdon Life Sciences 00

- 4205 PAGE 55

TABLE 5 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 56 MEAN 480 482 480 478

S.D. 40.3 45.0 40.4 46.6

N 26 26 25 26

DAY 63 MEAN 494 499 491 489

S.D. 42.2 48.3 41.4 51.3

N 26 26 25 26

DAY 69 MEAN 506 511 503 496

S.D. 45.5 49.5 44.8 58.8

N 26 26 25 26

DAY 77 MEAN 516 520 513 509

S.D. 46.4 50.4 44.7 55.5

N 26 26 25 26

DAY 84 MEAN 528 535 527 526

S.D. 49.7 51.5 45.8 58.1

N 26 26 25 26

DAY 91 MEAN 535 543 534 536

S.D. 52.0 53.6 47.5 57.7

N 26 26 25 26

DAY 98 MEAN 549 554 545 545

S.D. 54.4 55.1 50.8 59.7

N 26 26 25 26

DAY 105 MEAN 557 562 557 555

S.D. 55.9 56.0 49.6 61.8

N 26 26 25 26

DAY 112 MEAN 570 572 570 566

S.D. 57.0 58.0 51.6 63.8

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 11:54

Huntingdon Life Sciences 00

- 4205 PAGE 56

TABLE 5 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY

- 7 MEAN 109 109 109 109

S.D. 7.5 7.9 7.8 7.6

N 26 26 26 26

DAY 0 MEAN 155 154 150 152

S.D. 8.9 10.3 9.3 10.1

N 26 26 26 26

DAY 7 MEAN 178 179 175 175

S.D. 11.2 11.0 11.5 12.5

N 26 26 26 26

DAY 14 MEAN 204 201 199 198

S.D. 13.7 18.2 13.2 16.2

N 26 26 26 26

DAY 21 MEAN 224 223 220 215

S.D. 15.6 14.0 14.8 18.8

N 26 26 26 26

DAY 28 MEAN 240 238 233 229

S.D. 17.0 18.9 16.2 21.1

N 26 26 26 26

DAY 35 MEAN 248 251 247 242

S.D. 21.0 19.8 18.4 21.8

N 26 26 26 26

DAY 42 MEAN 258 262 259 253

S.D. 24.5 20.5 19.1 23.8

N 26 26 26 26

DAY 49 MEAN 268 268 264 261

S.D. 20.3 19.7 19.9 25.3

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 1 1:54

Huntingdon Life Sciences 00

- 4205 PAGE 57

TABLE 5 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 56 MEAN 273 275 273 267

S.D. 21.2 20.4 20.3 25.7

N 26 26 26 26

DAY 63 MEAN 278 278 276 273

S.D. 21.3 23.1 22.5 27.3

N 26 26 26 26

DAY 69 MEAN 283 283 279 278

S.D. 20.6 21.0 20.1 28.7

N 26 26 26 26

DAY 77 MEAN 264 282 296 280

S.D. 0.0 6.6 22.6 20.2

N 1 3 2 4

DAY 84 MEAN 268 294 301 291

S.D. 0.0 14.9 10.0 29.6

N 1 3 2 4

DAY 91 MEAN 272 303 293 314

S.D. 0.0 29.0 19.4 44.5

N 1 3 2 4

DAY 98 MEAN 284 292 310 285

S.D. 18.2 14.5 16.7 29.9

N 2 2 3 3

DAY 105 MEAN 288 299 311 290

S .D. 8.6 7.1 19.8 29.5

N 2 2 3 3

DAY 112 MEAN 310 307

S.D. 0.0 0.0

N 1 1

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 11:54

Huntingdon Life Sciences 00

- 4205 PAGE 58

TABLE 5 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT VALUES (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 119 MEAN 311 312

S.D. 0.0 0.0

N 1 1

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 11:56

Huntingdon Life Sciences 00

- 4205 PAGE 59

TABLE 6 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY

- 7 TO 0 MEAN 68 67 67 67

S.D. 7.8 5.0 7.7 6.2

N 26 26 26 26

DAY 0 TO 7 MEAN 54 53 56 53

S.D. 5.7 6.4 5.6 7.2

N 26 26 26 26

DAY 7 TO 14 MEAN 52 50 52 52

S .D. 7.6 6.3 8.5 8.4

N 26 26 26 26

DAY 14 TO 21 MEAN 41 42 35 40

S.D. 7.2 6.7 22.7 6.6

N 26 26 26 26

DAY 21 TO 28 MEAN 30 31 29 29

S.D. 10.5 6.3 6.8 6.6

N 26 26 25 26

DAY 28 TO 35 MEAN 29 32 30 28

S.D. 6.1 7.0 7.3 7.4

N 26 26 25 26

DAY 35 TO 42 MEAN 22 26 23 24

S.D. 22.2 5.7 5.2 9.3

N 26 26 25 26

-----------------------------------------------------------------------------------------------------------------------------------

No stati stically significant differences11

- JAN

- 2010 11:56

Huntingdon Life Sciences 00

- 4205 PAGE 60

TABLE 6 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

---------------------------------------------------------------------------------------------------------------- -------------------

DAY 42 TO 49 MEAN 22 19 19 20

S.D. 16.8 6.5 6.8 7.3

N 26 26 25 26

DAY 49 TO 56 MEAN 18 16 21 19

S.D. 7.5 4.3 5.3 8.5

N 26 26 25 26

DAY 56 TO 63 MEAN 13 17 11 11

S.D. 9.5 5.8 5.3 12.3

N 26 26 25 26

DAY 63 TO 69 MEAN 12 12 12 7

S.D. 8.3 5.1 5.4 24.3

N 26 26 25 26

DAY 69 TO 77 MEAN 10 9 9 13

S.D. 9.5 4.9 4.3 15.6

N 26 26 25 26

DAY 77 TO 84 MEAN 12 15 14 16

S.D. 9.9 5.1 5.5 6.1

N 26 26 25 26

DAY 84 TO 91 MEAN 7 8 8 10

S.D. 5.1 6.4 6.2 6.5

N 26 26 25 26

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 11:56

Huntingdon Life Sciences 00

- 4205 PAGE 61

TABLE 6 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

M ALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 91 TO 98 MEAN 14 11 11 9

S.D. 4.8 6.3 6.1 6.4

N 26 26 25 26

DAY 98 TO 105 MEAN 8 8 12* 10

S.D. 5.9 5.0 6.5 5.8

N 26 26 25 26

DAY 105 TO 112 MEAN 13 9* 12 10

S.D . 5.7 4.3 5.6 5.6

N 26 26 24 26

DAY 0 TO 69 MEAN 292 299 291 284

S.D. 38.2 41.2 37.6 50.6

N 26 26 25 26

DAY 0 TO 112 MEAN 356 360 358 353

S.D. 48.5 49.7 44.5 54.8

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0511

- JAN

- 2010 11:56

Huntingdon Life Sciences 00

- 4205 PAGE 62

TABLE 6 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY

- 7 TO 0 MEAN 46 45 42 43

S.D. 7.1 6.5 6.8 6.5

N 26 26 26 26

DAY 0 TO 7 MEAN 24 25 25 23

S.D. 5.1 5.9 5.8 6.0

N 26 26 26 26

DAY 7 TO 14 MEAN 26 22 24 24

S.D. 5.8 15.2 6.1 6.2

N 26 26 26 26

DAY 14 TO 21 MEAN 20 22 21 17

S.D. 4.4 10.8 5.2 6.2

N 26 26 26 26

DAY 21 TO 28 MEAN 15 15 12 14

S.D. 5.1 8.3 6.7 7.9

N 26 26 26 26

DAY 28 TO 35 MEAN 8 13 14** 12

S.D. 6.8 7.6 6.8 4.8

N 26 26 26 26

DAY 35 TO 42 MEAN 10 11 13 12

S.D. 14.0 5.7 5.2 6.1

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0111

- JAN

- 2010 11:56

Huntingdon Life Sciences 00

- 4205 PAGE 63

TABLE 6 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REP RODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 42 TO 49 MEAN 10 6 5 8

S.D. 11.8 5.6 4.3 6.7

N 26 26 26 26

DAY 49 TO 56 MEAN 5 7 9 6

S.D. 5.5 5.6 6.0 6.8

N 26 26 26 26

DAY 56 TO 63 MEAN 5 4 3 5

S.D. 5.8 7.4 5.8 6.3

N 26 26 26 26

DA Y 63 TO 69 MEAN 5 4 3 5

S.D. 5.1 6.7 7.6 5.3

N 26 26 26 26

DAY 0 TO 69 MEAN 129 129 129 126

S.D. 16.4 16.6 15.2 22.7

N 26 26 26 26

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 11:58

Huntingdon Life Sciences 00

- 4205 PAGE 64

TABLE 7 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF GESTATION BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 MEAN 280 280 278 275

S.D. 19.3 24.9 22.4 28.8

N 22 23 24 22

DAY 4 MEAN 301 304 301 299

S.D. 20.7 23.5 25.0 32.1

N 22 22 24 22

DAY 7 MEAN 309 313 312 309

S .D. 21.6 23.9 26.8 33.7

N 22 22 24 22

DAY 14 MEAN 336 340 339 337

S.D. 24.4 24.7 28.2 35.5

N 22 22 24 22

DAY 20 MEAN 392 396 393 397

S.D. 31.2 30.7 33.6 41.6

N 22 22 24 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:00

Huntingdon Life Sciences 00

- 4205 PAGE 65

TABLE 8 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF GESTATION BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 0 TO 4 MEAN 22 21 23 23

S.D. 5.4 5.3 6.0 7.0

N 22 22 24 22

DAYS 4 TO 7 MEAN 8 9 11 11

S.D. 4.2 3.8 3.2 6.7

N 22 22 24 22

DAYS 7 TO 14 MEAN 26 27 27 28

S .D. 5.4 3.8 4.6 10.2

N 22 22 24 22

DAYS 14 TO 20 MEAN 56 56 54 60

S.D. 14.3 13.2 18.7 11.4

N 22 22 24 22

DAYS 0 TO 20 MEAN 113 113 115 122

S.D. 20.0 15.5 20.4 17.8

N 22 22 24 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:00

Huntingdon Life Sciences 00

- 4205 PAGE 66

TABLE 9 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MATERNAL LACTATION BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 1 MEAN 309 304 306 304

S.D. 24.6 22.2 27.8 31.5

N 22 23 24 23

DAY 4 MEAN 317 323 321 321

S.D. 28.5 23.4 27.3 27.5

N 21 23 23 23

DAY 7 MEAN 318 329 328 329

S .D. 35.4 20.9 23.4 28.2

N 21 23 23 23

DAY 14 MEAN 340 345 346 348

S.D. 24.0 19.4 22.6 30.1

N 21 23 23 23

DAY 21 MEAN 331 334 343 343

S.D. 21.8 21.0 23.1 28.3

N 21 23 23 22

DAY 28 MEAN 308 312 310 311

S.D. 24.1 23.3 22.3 29.9

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:01

Huntingdon Life Sciences 00

- 4205 PAGE 67

TABLE 10 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 1 TO 4 MEAN 7 19* 16 16

S.D. 21.1 8.5 11.2 12.4

N 21 23 23 23

DAYS 4 TO 7 MEAN 1 6 7 8

S.D. 12.5 9.1 6.7 7.5

N 21 23 23 23

DAYS 7 TO 14 MEAN 22 15 18 19

S .D. 22.5 6.6 8.0 8.9

N 21 23 23 23

DAYS 14 TO 21 MEAN

- 9

- 11

- 3

- 4

S.D. 14.2 13.2 7.5 9.9

N 21 23 23 22

DAYS 21 TO 28 MEAN

- 23

- 22

- 34*

- 32

S.D. 15.8 17.3 11.3 10.5

N 21 23 23 22

DAYS 1 TO 28 MEAN

- 1 8 4 8

S.D. 12.5 15.8 14.8 17.1

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0511

- JAN

- 2010 12:22

Huntingdon Life Sciences 00

- 4205 PAGE 68

TABLE 11 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 MEAN 124 126 128** 130**

S.D. 4.6 5.0 5.6 6.1

N 26 26 25 26

DAY 7 MEAN 100 99 100 98

S.D. 4.1 4.8 3.4 4.0

N 26 26 26 26

DAY 14 MEAN 89 86* 87 85**

S .D. 4.2 3.4 3.2 3.6

N 25 26 26 26

DAY 21 MEAN 79 77 75 76

S.D. 5.3 3.1 8.6 7.3

N 26 26 26 26

DAY 28 MEAN 70 71 69 70

S.D. 4.5 3.2 3.7 4.7

N 26 26 25 25

DAY 35 MEAN 67 66 67 66

S.D. 2.9 2.1 1.9 3.2

N 26 26 25 26

DAY 42 MEAN 63 63 64 63

S.D. 8.0 2.4 2.6 3.4

N 26 26 25 26

DAY 49 MEAN 63 60 62 61

S.D. 5.5 2.6 2.3 3.7

N 25 26 24 26

DAY 56 MEAN 59 55** 57 57

S.D. 4.1 5.1 2.7 3.0

N 26 26 25 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 12:22

Huntingdon Life Sciences 00

- 4205 PAGE 69

TABLE 11 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

MALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 63 MEAN 55 55 54 54

S.D. 3.2 2.4 3.2 5.8

N 26 26 25 26

DAY 69 MEAN 54 53 54 52

S.D. 5.0 2.2 2.2 7.8

N 26 26 25 26

DAY 91 MEAN 50 47** 49 49

S.D. 2.4 2.7 1.9 2.6

N 26 26 25 26

DAY 98 MEAN 49 47* 48 48

S.D. 2.5 2.9 2.1 2.7

N 26 26 25 26

DAY 105 MEAN 48 47 49 49

S.D. 3.0 3.2 3.2 2.5

N 26 26 25 26

DAY 112 MEAN 47 46 48 47

S.D. 3.0 2.6 2.2 2.7

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 12:22

Huntingdon Life Sciences 00

- 4205 PAGE 70

TABLE 11 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BOD Y

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 0 MEAN 127 135** 131 131

S.D. 9.8 7.2 6.2 7.6

N 26 26 25 26

DAY 7 MEAN 104 103 105 101

S.D . 8.1 7.1 6.3 6.6

N 26 26 22 25

DAY 14 MEAN 96 92 94 91*

S.D. 6.5 10.1 5.6 6.6

N 26 26 25 25

DAY 21 MEAN 87 87 86 83*

S.D. 4.9 8.1 4.4 4.6

N 26 26 26 26

DAY 28 MEAN 84 83 8 3 81

S.D. 5.6 5.5 4.0 7.6

N 26 26 24 26

DAY 35 MEAN 80 78 80 77

S.D. 5.1 4.2 4.2 3.3

N 25 24 26 26

DAY 42 MEAN 76 76 80 75

S.D. 9.9 5.5 3.9 4.9

N 26 26 26 25

DAY 49 MEAN 77 74 76 74

S.D. 7.4 5.4 4.6 3.6

N 26 25 26 26

DAY 56 MEAN 70 71 72 70

S.D. 3.8 4.8 5.3 4.9

N 26 25 26 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 12:22

Huntingdon Life Sciences 00

- 4205 PAGE 71

TABLE 11 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

FEMALES MEAN FEED CONSUMPTION ( GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 63 MEAN 66 67 68 70

S.D. 4.2 4.6 6.4 9.9

N 26 26 26 26

DAY 69 MEAN 65 64 66 65

S.D. 4.1 5.6 4.4 4.0

N 26 26 26 25

DAY 91 MEAN 57 64 54 59

S.D. 0.0 6.6 0.4 1.7

N 1 3 2 4

DAY 98 MEAN 54 53 54 56

S.D. 0.0 0.4 4.6 3.0

N 1 2 2 3

DAY 105 MEAN 54 56 54 55

S.D. 4.4 2.6 3.2 2.7

N 2 2 3 3

DAY 112 MEAN 110 130

S.D. 0.0 0.0

N 1 1

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:23

Huntingdon Life Sciences 00

- 4205 PAGE 72

TABLE 12 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF GESTATION FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 0 TO 4 MEAN 72 72 74 72

S.D. 5.3 8.2 5.3 5.8

N 22 21 24 22

DAYS 4 TO 7 MEAN 75 73 76 73

S.D. 4.3 7.0 6.2 5.9

N 22 22 24 22

DAYS 7 TO 14 MEAN 71 71 71 70

S .D. 3.9 4.4 3.5 5.5

N 22 22 24 22

DAYS 14 TO 20 MEAN 64 61 61 62

S.D. 3.4 3.8 4.3 3.9

N 22 22 23 22

DAYS 0 TO 20 MEAN 70 69 71 69

S.D. 3.3 4.9 3.7 4.1

N 22 22 24 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:25

Huntingdon Life Sciences 00

- 4205 PAGE 73

TABLE 13 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF LACTATION FEED CONSUMPTION (GRAMS/KG/DAY)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAYS 1 TO 4 MEAN 90 93 104* 99

S.D. 19.6 15.0 15.6 16.8

N 21 23 23 2 2

DAYS 4 TO 7 MEAN 108 111 116 11 3

S.D. 14.1 13.4 12.8 11.2

N 19 23 23 2 1

DAYS 7 TO 14 MEAN 135 136 143 140

S .D. 13.7 15.2 9.4 10.1

N 21 23 23 2 2

DAYS 14 TO 21 MEAN 178 183 190 187

S.D. 17.3 21.2 17.3 21.6

N 21 22 23 21

DAYS 21 TO 28 MEAN 324 341 344 337

S.D. 60.4 58.2 35.3 41.7

N 17 17 22 18

DAYS 1 TO 28 MEAN 161 161 178* 16 8

S.D. 20.9 25.8 16.3 22.8

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0511

- JAN

- 2010 12:30

Huntingdon Life Sciences 00

- 4205 PAGE 74

TABLE 14 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ESTROUS STAGES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

PRE

- COHABITATION PERIOD (21 days evaluated)

FEMALES EXAMINED N 26 26 26 26

CYCLE LENGTH (days) MEAN 4.2 4.2 4.2 4. 0

S.D. 0.42 0.3 7 0.32 0.73

N 26 26 26 25

NUMBER of ESTROUS CYCLES MEAN 4.2 4.0 4.1 4.0

S.D. 0.54 0.66 0.59 0. 17

N 26 26 26 25

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:31

Huntingdon Life Sciences 00

- 4205 PAGE 75

TABLE 15 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF COHABITATION DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Females paired with males N 26 26 26 26

Males placed with females N 26 26 25 26

Total number mated N 25 24 23 23

male mating index % 96.2 92.3 92.0 88.5

with females pregnant N 22 24 a 23 23

male fertilit y index % 84.6 92.3 92.0 88.5

Females with defined

day 0 of Gestation N 25 23 24 22

No. of days until Mating MEAN 2.4 3.0 3.2 3.1

S.D. 1.12 1.48 1.28 1.70

Day 1 to 4 N 24 22 21 18

% 96.0 95.7 87.5 81.8

Day 5 to 8 N 1 1 3 4

% 4.0 4.3 12.5 18.2

Day 9 to 14 N 0 0 0 0

% 0.0 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences

a Includes Female No. 2536 which had positive signs of mating and was sacrificed for humane reasons on Gestation Day 1 due to a

snout injury. This animal was presumed pregnant for calculation purposes although this cannot be confirmed.22

- JAN

- 2010 11:58

Huntingdon Life Sciences 00

- 4205 PAGE 76

TABLE 16 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Females on Study N 26 26 26 25

Females Mated N 25 24 24 2 2

Mating Index % 96.2 92.3 92.3 88. 0

F emales Pregnant N 22 24 a 24 2 2

Female Fertility Index % 88.0 100.0 100.0 100.0

Females with L iveborn N 22 23 24 2 2

Gestation Index % 100.0 95.8 100.0 100.0

Females Completing Delivery N 22 23 24 2 2

% 100.0 95.8 100.0 100.0

with Stillborn Pups N 4 2 2 5

% 18.2 8.7 8.3 2 2.7

with all Stillborn N 0 0 0 0

% 0.0 0.0 0.0 0.0

Litters with Liveborn,

but no Pups Alive

day 4 N 1 0 1 0

% 4.5 0.0 4.2 0.0

day 21 N 1 0 1 0

% 4.5 0.0 4.2 0.0

Duration of Gestation MEAN 21.9 22.0 21.9 22.0

S.D. 0.29 0.31 0.41 0.4 5

N 22 22 24 2 1

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences

a Includes Female No. 2536 which had positive signs of mating and was sacrificed for humane reasons on Gestation Day 1 due to a

snout injury. This animal was presumed pregnant for calculation purposes although this cannot be confirmed.22

- JAN

- 2010 11:58

Huntingdon Life Sciences 00

- 4205 PAGE 77

TABLE 16 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRO DUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters with Liveborn Pups N 22 23 24 2 2

Pups Delivered (total) N 266 301 314 287

MEAN 12.1 13.1 13.1 13. 0

S.D. 2.81 2.70 2.95 2.0 8

Liveborn N 262 299 312 2 80

Live Birth Index % 98.5 99.3 99.4 97. 6

Stillborn N 4 2 2 7

% 1.5 0.7 0.6 2. 4

Culled day 4 46 71 78 6 2

Liv eborn, not culled N 216 228 234 2 1 8

prior to day 21

Pups Dying, Missing, and/or Cannibalized

day 0 N 0 2 0 2

% 0.0 0.7 0.0 0.7

days 1

- 4 N 18 3 ** 4** 2**

% 6.9 1.0 1.3 0.7

days 5

- 21 N 2 0 2 0

% 0.8 0.0 0.6 0.0

days 0

- 4 N 18 5** 4** 4**

% 6.9 1.7 1.3 1.4

days 0

- 21 N 20 5** 6** 4**

% 7.6 1.7 1.9 1.4

Pups Surviving 4 days N 244 294** 308** 2 76**

Viability Index % 93.1 98.3 98.7 98.6

Pups Surviving 21 days N 196 223 228 214

Lactation Index % 9 9.0 100.0 99.1 100.0

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0122

- JAN

- 2010 11:58

Huntingdon Life Sciences 00

- 4205 PAGE 78

TABLE 16 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Implantation Sites N 280 325 342 319

per Litter MEAN 12.7 14.1 14.3 14.5

S.D. 3.21 2.60 2.86 1.71

Live Pups/Litter

day 1 MEAN 11.8 12.8 13.4 12.6

S.D. 2.76 2.77 1.64 2.34

N 22 23 23 22

day 4 preculling MEAN 11.1 12.8 13.4* 12.5

S.D. 3.70 2.80 1.64 2.44

N 22 23 23 22

day 4 postculling MEAN 9.0 9.7 10.0 9.7

S.D. 2.43 1.26 0.00 0.77

N 22 23 23 22

day 7 MEAN 9.0 9.7 10.0 9.7

S.D. 2.43 1.26 0.00 0.77

N 22 23 23 22

day 14 MEAN 8.9 9.7 10.0 9.7

S.D. 2.45 1.26 0.00 0.77

N 22 23 23 22

day 21 MEAN 8.9 9.7 9.9 9.7

S.D. 2.45 1.26 0.29 0.77

N 22 23 23 22

day 28 MEAN 8.9 9.7 9.9 9.7

S.D. 2.45 1.26 0.29 0.77

N 22 23 23 22

Sex Ratio

- Male Pups:Total Pups

day 0 N 133 152 161 150

% 50.8 50.8 51.6 53.8

day 21 N 99 112 120 111

% 50.5 50.2 52.6 51.9

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.0522

- JAN

- 2010 11:58

Huntingdon Life Sciences 00

- 4205 PAGE 79

TABLE 16 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF DELIVERY AND LITTER DATA

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Pup Weight/Litter (grams)

day 1 MEAN 7.4 7.2 7.0 7.3

S.D. 0.81 0.46 0.63 0.49

N 22 23 23 22

day 4 MEAN 11.4 10.4* 10.2** 10.5*

S.D. 1.38 1.18 1.16 0.95

N 21 23 23 22

day 7 MEAN 14.6 14.6 14.3 14.3

S.D. 2.25 1.36 1.51 1.09

N 21 23 23 22

day 14 MEAN 25.2 25.1 24.5 24.3

S.D. 3.39 2.01 2.37 1.82

N 21 23 23 22

day 21 MEAN 41.9 42.3 40.9 41.2

S.D. 5.89 4.20 5.19 3.99

N 21 23 23 22

day 28 MEAN 78.6 78.8 76.2 76.9

S.D. 7.92 6.60 8.22 5.85

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical ke y: * = p<0.05 ** = p<0.0111

- JAN

- 2010 12:35

Huntingdon Life Sciences 00

- 4205 PAGE 80

TABLE 1 7 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP CLINICAL OBSERVATIONS DURING LACTATION

- (frequency/animals)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

DAY 1 to 28

Normal

----------

WITHIN NORMAL LIMITS 1029/ 21 1184/23 1217/23 1166/23

Gen. Appearance

-------------------

SWOLLEN PAW(S) 1/ 1 0/ 0 0/ 0 0/ 0

DO ME HEAD 0/ 0 0/ 0 7/ 1 0/ 0

SWOLLEN SNOUT 0/ 0 1/ 1 0/ 0 0/ 0

Dermal

- General

------------------

ULCERATION

- CERVICAL 0/ 0 1/ 1 0/ 0 0/ 0

SCABS 1/ 1 1/ 1 0/ 0 0/ 0

Ocular

----------

OPACITY

- UNILATERAL 0/ 0 2/ 1 0/ 0 0/ 011

- JAN

- 2010 12:36

Huntingdon Life Sciences 00

- 4205 PAGE 81

TABLE 18 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 1 males MEAN 7.6 7.4 7.2 7.4

S.D. 0.81 0.45 0.66 0.55

N 22 23 23 22

1 females MEAN 7.1 7.0 6.8 7.1

S.D. 0.87 0.56 0.62 0.50

N 22 23 23 22

1 males+females MEAN 7.4 7.2 7.0 7.3

S .D. 0.81 0.46 0.63 0.49

N 22 23 23 22

day 4 males MEAN 11.7 10.6** 10.5** 10.7*

preculling S.D. 1.37 1.18 1.18 1.06

N 21 23 23 22

4 females MEAN 11.0 10.3 9.9* 10.3

preculling S.D. 1.46 1.39 1.13 0.92

N 21 23 23 22

4 males+females MEAN 11.4 10.4* 1 0.2** 10.5*

preculling S.D. 1.38 1.18 1.16 0.95

N 21 23 23 22

day 4 males MEAN 11.7 10.6** 10.6** 10.7*

postculling S.D. 1.37 1.18 1.17 1.12

N 21 23 23 22

4 females MEAN 11.0 10.3 9.9* 10.3

postcull ing S.D. 1.48 1.39 1.12 0.91

N 21 23 23 22

4 males+females MEA N 11.4 10.4* 10.3** 10.5*

postculling S.D. 1.41 1.19 1.15 0.99

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 12:36

Huntingdon Life Sciences 00

- 4205 PAGE 82

TABLE 18 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 7 males MEAN 15.0 14.8 14.6 14.7

S.D. 2.25 1.43 1.54 1.29

N 21 23 23 22

7 females MEAN 14.2 14.3 13.9 14.0

S.D. 2.30 1.60 1.47 0.97

N 21 23 23 22

7 males+females MEAN 14.6 14.6 14.3 14.3

S.D. 2.25 1.36 1.51 1.09

N 21 23 23 22

day 14 males MEAN 25.6 25.5 25.0 24.8

S.D. 3.39 2.23 2.43 2.03

N 21 23 23 22

14 females MEAN 24.8 24.7 23.9 23.9

S.D. 3.52 2.21 2.35 1.70

N 21 23 23 22

14 m ales+females MEAN 25.2 25.1 24.5 24.3

S.D. 3.39 2.01 2.37 1.82

N 21 23 23 22

day 21 males MEAN 42.7 42.8 41.6 42.1

S.D. 5.99 4.73 5.54 4.66

N 21 23 23 22

21 females MEAN 41.2 41.7 40.1 40.5

S.D. 6.18 4.30 4.85 3.49

N 21 23 23 22

21 males+females MEAN 41.9 42.3 40.9 41.2

S.D. 5.89 4.20 5.19 3.99

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:36

Huntingdon Life Sciences 00

- 4205 PAGE 83

TABLE 18 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MEAN PUP BODY WEIGHTS (GRAMS)

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 28 males MEAN 81.8 81.2 79.1 80.0

S.D. 8.95 7.83 8.91 7.19

N 21 23 23 22

28 females MEAN 75.5 76.2 73. 0 73.8

S.D. 7.72 6.37 7.55 4.96

N 21 23 23 22

28 males+females MEAN 78.6 78.8 76.2 76.9

S.D. 7.92 6.60 8.22 5.85

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statist ically significant differences11

- JAN

- 2010 12:36

Huntingdon Life Sciences 00

- 4205 PAGE 84

TABLE 18 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ON E- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP BODY WEIGHT GAIN

-- GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

------------------------------------------------------------------------------------------------------------------ -----------------

day 1

- 4 males MEAN 4.0 3.2** 3.3* 3.3*

S.D. 0.90 0.90 0.67 0.83

N 21 23 23 22

females MEAN 3.8 3.2 3.1* 3.2*

S.D. 0.90 0.97 0.66 0.56

N 21 23 23 22

males+females MEAN 3.9 3.2* 3.2* 3.2*

S.D. 0.88 0.87 0.66 0.62

N 21 23 23 22

day 4

- 7 males MEAN 3.3 4.2* 4.1* 3.9

S.D. 1.81 0.73 0.69 0.67

N 21 23 23 22

females MEAN 3.1 4.0* 4.0* 3.8

S.D. 1.78 0.67 0.62 0.61

N 21 23 23 22

males+females MEAN 3.2 4.1** 4.0* 3.9

S.D. 1.80 0.66 0.65 0.63

N 21 23 23 22

day 4

- 21 males MEAN 31.0 32.2 31.1 31.4

S.D. 5.44 4.21 4.71 4.03

N 21 23 23 22

fem ales MEAN 30.2 31.4 30.1 30.2

S.D. 5.57 3.54 4.02 3.12

N 21 23 23 22

males+females MEAN 30.5 31.9 30.7 30.8

S.D. 5.34 3.67 4.35 3.52

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 12:36

Huntingdon Life Sciences 00

- 4205 PAGE 85

TABLE 18 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMM ARY OF PUP BODY WEIGHT GAIN

-- GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 4

- 28 males MEAN 70.1 70.6 68.5 69.2

S.D. 8.29 7.30 8.13 6.51

N 21 23 23 22

females MEAN 6 4.5 65.9 63.1 63.6

S.D. 6.99 5.70 6.82 4.56

N 21 23 23 22

males+females MEAN 67.2 68.4 66.0 66.4

S.D. 7.23 6.08 7.45 5.35

N 21 23 23 22

day 7

- 14 males MEAN 10.6 10.6 10.4 10.1

S.D. 1.46 1.29 1.29 1.22

N 21 23 23 22

females MEAN 10.7 10.4 10.0 9.9

S.D. 1.70 1.13 1.34 1.17

N 21 23 23 22

males+females MEAN 10.6 10.5 10.2 10.0

S.D. 1.49 1.15 1.28 1.19

N 21 23 23 22

day 7

- 28 males MEAN 66.8 66.4 64.5 65.3

S.D. 7.02 6.79 7.68 6.19

N 21 23 23 22

females MEAN 61.4 61.9 59.1 59.8

S.D. 5.98 5.35 6.40 4.24

N 21 23 23 22

males+females MEAN 64.0 64.3 62.0 62.6

S.D. 6.01 5.66 7.01 5.03

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:36

Huntingdon Life Sciences 00

- 4205 PAGE 86

TABLE 18 F 1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION T OXICITY STUDY IN RATS

SUMMARY OF PUP BODY WEIGHT GAIN

-- GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

day 14

- 21 males MEAN 17.1 17.3 16.6 17.3

S.D. 3.12 2.78 3.32 2.99

N 21 23 23 22

females MEAN 16.4 17.0 16.1 16.6

S.D. 3.18 2.46 2.90 2.17

N 21 23 23 22

males+females MEAN 16.7 17.2 16.4 16.9

S.D. 3.02 2.50 3.10 2.53

N 21 23 23 22

day 14

- 28 males MEAN 56.3 55.7 54.1 55.2

S.D. 5.79 5.86 6.68 5.46

N 21 23 23 22

females MEAN 50.7 51.5 49.1 49.9

S.D. 4.52 4.65 5.60 3.55

N 21 23 23 22

males+females MEAN 53.4 53.7 51.8 52.6

S.D. 4.76 4.93 6.12 4.31

N 21 23 23 22

day 21

- 28 males MEAN 39.2 38.4 37.5 37.9

S.D. 3.71 3.60 3.86 2.94

N 21 23 23 22

females MEAN 34.3 34.5 32.9 33.3

S.D. 2.25 2.82 3.21 2.17

N 21 23 23 22

males+females MEAN 36.7 36.6 35.3 35.7

S.D. 2.74 3.00 3.50 2.34

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically signif icant differences11

- JAN

- 2010 12:38

Huntingdon Life Sciences 00

- 4205 PAGE 87

TABLE 19 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26

GROSS EXAM N 0 0 1 0

----------

FRACTURE(S) N 0 0 1 0

% 0.0 0.0 3.8 0.0

THYMUS N 1 3 2 1

--- ---

DISCOLORED FOCUS/FOCI N 1 3 2 1

% 3.8 11.5 7.7 3.8

LUNGS N 1 3 5 6

-----

DISCOLORED FOCI N 1 3 5 6

% 3.8 11.5 19.2 23.1

SPLEEN N 0 0 1 0

------

ENLARGED N 0 0 1 0

% 0.0 0.0 3.8 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:38

Hunting don Life Sciences 00

- 4205 PAGE 88

TA BLE 19 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26

LIVER N 2 0 2 0

-----

ENLARGED N 0 0 1 0

% 0.0 0.0 3.8 0.0

DISCOLORED FOCI N 1 0 0 0

% 3.8 0.0 0.0 0.0

DISCOLORED AREA(S) N 1 0 0 0

% 3.8 0.0 0.0 0.0

ADHESION N 0 0 1 0

% 0.0 0.0 3.8 0.0

NODULE(S) N 0 0 1 0

% 0.0 0.0 3.8 0.0

KIDNEY N 3 3 4 3

------

DILATED RENAL PELVIS N 3 3 3 2

% 11.5 11.5 11.5 7.7

DISCOLORED FOCUS/FOCI N 0 0 0 1

% 0.0 0.0 0.0 3.8

CYSTIC KIDNEY N 0 0 1 0

% 0.0 0.0 3.8 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:38

Huntingdon Life Sciences 00

- 4205 PAGE 89

TABLE 19 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSAT E: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

MALES N 26 26 26 26

GONADS N 0 1 0 1

------

DISCOLORED FOCI ON PROSTATE N 0 1 0 1

% 0.0 3.8 0.0 3.8

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:38

Huntingdon Life Sciences 00

- 4205 PAGE 90

TABLE 19 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

FEMALES N 26 26 26 26

GROSS EXAM N 0 1 0 1

----------

FRACTURE(S) N 0 1 0 0

% 0.0 3.8 0.0 0.0

HEMORRHAGE N 0 0 0 1

% 0.0 0.0 0.0 3.8

EYES N 0 1 0 0

----

OPACITY N 0 1 0 0

% 0.0 3.8 0.0 0.0

THYMUS N 0 0 1 0

------

DISCOLORED FOCUS/FOCI N 0 0 1 0

% 0.0 0.0 3.8 0.0

THORACIC CAVITY N 1 0 0 0

---------------

ENLARGED LYMPH NO DE(S) N 1 0 0 0

% 3.8 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 12:38

Huntingdon Life Sciences 00

- 4205 PAGE 91

TABLE 19 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PARENTAL NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

---------------------------------------------------------------------------------------------------------------------------------- -

FEMALES N 26 26 26 26

LUNGS N 4 7 11 10

-----

DISCOLORED FOCI N 4 7 11 10

% 15.4 26.9 42.3 38.5

SPLEEN N 0 0 0 1

------

ADHESION N 0 0 0 1

% 0.0 0.0 0.0 3.8

ADRENALS N 1 0 0 0

--------

DISCOLORED ADRENALS N 1 0 0 0

% 3.8 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 92

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

Stillborn N 4 2 2 7

GROSS EXAM

----------

Litter Incidence N 4 1 3 0

Pup Incidence N 15 1 3 0

CANNIBALIZED

Pup Incidence N 1 1 1 0

% 0.5 0.5 0.5 0.0

Litter Incidence N 1 1 1 0

% 4.5 4.3 4.2 0.0

SEX UNDETERMINED

Pup Incidence N 0 0 1 0

% 0.0 0.0 0.5 0.0

Litter Incidence N 0 0 1 0

% 0.0 0.0 4.2 0.0

AUTOLYSIS

Pup Incidence N 14 0 3 0

% 7.4 0.0 1.4 0.0

Litter Incidence N 4 0 3 0

% 18.2 0.0 12.5 0.0

EYES

----

Litter Incidence N 0 0 2 0

Pup Incidence N 0 0 2 0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 93

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP NECROPSY OBSERVATIONS

----------------------------------------------------------------------------------------------------------------------------- ----- -

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

Stillbo rn N 4 2 2 7

OPACITY

Pup Incidence N 0 0 2 0

% 0.0 0.0 0.9 0.0

Litter Incidence N 0 0 2 0

% 0.0 0.0 8.3 0.0

THYMUS

------

Litter Incidence N 1 0 0 1

Pup Incidence N 1 0 0 1

DISCOLORED FOCI

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 0.5

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 4.5

SMALL

Pu p Incidence N 1 0 0 0

% 0.5 0.0 0.0 0.0

Litter Incidence N 1 0 0 0

% 4.5 0.0 0.0 0.0

LUNGS

-----

Litter Incidence N 3 4 2 5

Pup Incidence N 3 4 2 8

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 94

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

Stillborn N 4 2 2 7

LUNG FLOTATION TEST

- STILLBORN

Pup Incidence N 3 2 1 7

% 1.6 0.9 0.5 3.5

Litter Incidence N 3 2 1 5

% 13.6 8.7 4.2 22.7

LUNG FLOTATION TEST

- FOUND DEAD

Pup Incidence N 0 2 1 1

% 0.0 0.9 0.5 0.5

Litter Incidence N 0 2 1 1

% 0.0 8.7 4.2 4.5

STOMACH

-------

Litter Incidence N 3 4 2 5

Pup Incidence N 6 4 2 8

NO MILK IN STOMACH

Pup Inciden ce N 5 3 2 8

% 2.6 1.4 0.9 4.0

Litter Incidence N 3 3 2 5

% 13.6 13.0 8.3 22.7

SMALL

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 0.5

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 4.5

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 95

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

St illborn N 4 2 2 7

MILK IN STOMACH

Pup Incidence N 1 1 0 0

% 0.5 0.5 0.0 0.0

Litter Incidence N 1 1 0 0

% 4.5 4.3 0.0 0.0

KIDNEY

------

Litter Incidence N 4 3 3 3

Pup Incidence N 4 4 4 4

M FUSED KIDNEY

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 0.5

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 4.5

ENLARGED KIDNEY(S)

Pup Incidence N 0 0 1 0

% 0.0 0.0 0.5 0.0

Litter Incidence N 0 0 1 0

% 0.0 0.0 4.2 0.0

CYST(S)

Pup Incidence N 1 1 3 0

% 0.5 0.5 1.4 0.0

Litter Incidence N 1 1 2 0

% 4.5 4.3 8.3 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 96

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RA TS

SUMMARY OF PUP NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

Stillborn N 4 2 2 7

DILATED RENAL PELVIS

Pup Incidence N 3 3 1 3

% 1.6 1.4 0.5 1.5

Litter Incidence N 3 2 1 2

% 13.6 8.7 4.2 9.1

DISCOLORED

Pup Incidence N 0 0 1 0

% 0.0 0.0 0.5 0.0

Litter Incidence N 0 0 1 0

% 0.0 0.0 4.2 0.0

INTESTINES

----------

Litter Incidence N 0 0 0 1

Pup Incidence N 0 0 0 1

A UNDERDEVELOPMENT OF INTESTINE

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 0.5

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 4.5

ANAL ATRESIA

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 0.5

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 4.5

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 97

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TO XICITY STUDY IN RATS

SUMMARY OF PUP NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

Stillborn N 4 2 2 7

GONADS

------

Litter Incidence N 5 5 4 6

Pup Incidence N 8 8 4 6

UNDESCENDED TESTE(S)

Pup Incidence N 8 8 4 6

% 4.2 3.8 1.8 3.0

Litter Incidence N 5 5 4 6

% 22.7 21.7 16.7 27.3

ABDOMINAL CAVITY

----------------

Litter Incidence N 1 0 0 1

Pup Incidence N 1 0 0 1

GASTROSCHISIS

Pup Incidence N 0 0 0 1

% 0.0 0.0 0.0 0.5

Litter Incidence N 0 0 0 1

% 0.0 0.0 0.0 4.5

DIAPHRAGMAT IC HERNIA

Pup Incidence N 1 0 0 0

% 0.5 0.0 0.0 0.0

Litter Incidence N 1 0 0 0

% 4.5 0.0 0.0 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences22

- JAN

- 2010 12:34

Huntingdon Life Sciences 00

- 4205 PAGE 98

TABLE 20 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION T OXICITY STUDY IN RATS

SUMMARY OF PUP NECROPSY OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

Litters Evaluated N 22 23 24 22

Pups Evaluated N 190 211 221 201

Live N 186 209 219 194

Stillborn N 4 2 2 7

HEAD

----

Litter Incidence N 0 0 1 0

Pup Incidence N 0 0 1 0

V DOMED HEAD

Pup Incidence N 0 0 1 0

% 0.0 0.0 0.5 0.0

Litter Incidence N 0 0 1 0

% 0.0 0.0 4.2 0.0

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences

# Huntingdon Life Sciences 00

- 4205 Page 99

211

- DIPE

- 1G Final Report

Mean Parental Organ Weights

Preface Tab le 21

Table of Contents

P 0 

# Generation Absolute Organ Weights

........................................................... 100

P 0 

# Generation Organ to Body Weight Ratios

................................................... 104

P 0 

# Generation Organ to Brain Weight Ratios

................................................... 108

Key to Abbreviations:

g = Grams

Sem.Ves & Co.GL. = Seminal Vesicles and Coagulating Glands11

- JAN -2 010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 100

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ABSOLUTE ORGAN WEIGHTS

MALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

------------------------------------------------------------------------------------------------------------------ -----------------

FINAL BODY WEIGHT g MEAN 575 576 575 568

S.D. 58.0 59.5 53.0 64.9

N 26 26 24 26

TESTIS

- RIGHT g MEAN 1.7060 1.6767 1.7498 1.7651

S.D. .14280 .09950 .13300 .12232

N 26 26 24 26

TESTIS

- LEFT g MEAN 1.7065 1.6774 1.7594 1.7527

S.D. .15133 .08929 .14124 .12487

N 26 26 24 26

TESTES g MEAN 3.4053 3.3432 3.4960 3.5072

S.D. .29263 .17706 .26960 .24313

N 26 26 24 26

PROSTATE g MEAN 1.2088 1.14 42 1.2587 1.1935

S.D. .25577 .19400 .25284 .25560

N 26 26 24 26

SEM.VES & CO.GL. g MEAN 1.8887 1.8686 1.9957 2.1836**

S.D. .34371 .36861 .32072 .33852

N 26 26 24 26

EPIDIDYMIS RIGHT g MEAN 0.7065 0.7342 0.7542* 0.7485*

S.D. .06035 .05919 .05040 .06290

N 26 25 24 26

EPIDIDYMIS LEF T g MEAN 0.7025 0.7200 0.7507* 0.7339

S.D. .06496 .05654 .05279 .06036

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 101

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ABSOLUTE ORGAN WEIGHTS

MALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

---------------------------------------------------------------------------------------------------------------------------------- -

EPIDIDYMIDES g MEAN 1.3979 1.4429 1.4969** 1.4742*

S.D. .11588 .09958 .09790 .10475

N 26 26 24 26

ADRENALS g MEAN 0.0632 0.0646 0.0680 0.0607

S.D. .00800 .01165 .01189 .01061

N 26 26 24 26

BRAIN g MEAN 2.1742 2.1648 2.1704 2.1301

S.D. .10816 .06601 .08024 .08252

N 26 26 24 26

KIDNEYS g MEAN 4.1123 4.0631 4.6034** 4.7256**

S.D. .51983 .38374 .47749 .53666

N 26 26 24 26

LIVER g MEAN 19.337 19.551 20.315 21.225*

S.D. 2.3574 2.3028 2.6331 2.5354

N 26 26 24 26

LUNGS g MEAN 1.8452 1.8113 1.8679 1.82 36

S.D. .26034 .18217 .21915 .15627

N 26 26 24 26

PITUITARY g MEAN 0.0120 0.0114 0.0120 0.0118

S.D. .00147 .00207 .00133 .00207

N 26 26 24 26

SPLEEN g MEAN 0.8145 0.7693 0.8396 0.7907

S.D. .13658 .10398 .12317 .10640

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 102

TABLE 21 P0 GENERA TION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ABSOLUTE ORGAN WEIGHTS

FEMALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG /M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

FINAL BODY WEIGHT g MEAN 308 312 310 311

S.D. 24.1 23.3 22.3 29.9

N 21 23 23 22

ADRENALS g MEAN 0.0734 0.0751 0.0728 0.0767

S.D. .0 0770 .01102 .01314 .01069

N 21 23 23 22

BRAIN g MEAN 1.9398 1.9219 1.9267 1.9395

S.D. .07778 .08865 .06155 .07416

N 21 23 23 22

KIDNEYS g MEAN 2.5653 2.5942 2.6382 2.6944

S.D. .18529 .18497 .19552 .25326

N 21 23 23 22

LIVER g MEAN 13.920 13.849 14.350 14.506

S.D. 1.6664 1.7315 1.5820 2.1402

N 21 23 23 22

LUNGS g MEAN 1.4551 1.4585 1.4780 1.4882

S.D. .10850 .12881 .11823 .16740

N 21 23 23 22

PITUITARY g MEAN 0.0143 0.0159 0.0145 0.0156

S.D. .00271 .00341 .00207 .00345

N 21 23 23 22

SPLEEN g MEAN 0.58 69 0.5995 0.6025 0.6148

S.D. .07949 .06532 .07758 .09543

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 103

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ABSOLUTE ORGAN WEIGHTS

FEMALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

OVARY RIGHT g MEAN 0.0577 0.0550 0.0565 0.0585

S.D. .01177 .01297 .00880 .01266

N 21 23 23 22

OVARY LEFT g MEAN 0.0529 0.0554 0.0532 0.0583

S.D. .01332 .01074 .00974 .01005

N 21 23 23 22

OVARIES g MEAN 0.1100 0.1100 0.1097 0.1161

S.D. .02113 .01881 .0 1548 .01902

N 21 23 23 22

UTERUS/OVIDUCTS g MEAN 0.7000 0.7036 0.6054 0.6729

S.D. .19015 .19208 .14815 .19628

N 21 23 23 22

-------------------------------------------------------------------------------------------------------------------------------- ---

No statistically significant differences11

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 104

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO

MALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

FINAL BODY WEIGHT g MEAN 575 576 575 568

S.D. 58.0 59.5 53.0 64.9

N 26 26 24 26

TESTIS

- RIGHT Ratio MEAN 0.0030 0.0029 0.0031 0.003 1

S.D. .00033 .00038 .00033 .00035

N 26 26 24 26

TESTIS

- LE FT Ratio MEAN 0.0030 0.0029 0.0031 0.0031

S.D. .00033 .00037 .00035 .00034

N 26 26 24 26

TESTES Ratio MEAN 0.0060 0.0059 0.0061 0.0062

S.D. .00066 .00073 .00068 .00069

N 26 26 24 26

PROSTATE Ratio MEAN 0.0021 0.0020 0.0022 0.0021

S.D. .00046 .00041 .00044 .00052

N 26 26 24 26

SEM.VES & CO.GL. Ratio MEAN 0.0033 0.0033 0.0035 0.0039**

S.D. .00061 .00065 .00064 .00076

N 26 26 24 26

EPIDIDYMIS RIGHT Ratio MEAN 0.0012 0.0013 0.0013 0.0013

S.D. .00015 .00017 .00014 .00015

N 26 25 24 26

EPIDIDYMIS LEFT Ratio MEAN 0.0012 0.0013 0.0013 0.0013

S.D. .00014 .00014 .00016 .00014

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0111

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 105

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENER ATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO

MALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

EPIDIDYMIDES Ratio MEAN 0.0024 0.0025 0.0026 0 .0026

S.D. .00028 .00029 .00029 .00028

N 26 26 24 26

ADRENAL S Ratio MEAN 0.0001 0.0001 0.0001 0.0001

S.D. .00002 .00002 .00002 .00002

N 26 26 24 26

BRAIN Ratio MEAN 0.0038 0.0038 0.0038 0.0038

S.D. .00034 .00039 .00032 .00035

N 26 26 24 26

KIDNEYS Ratio MEAN 0.0071 0.0071 0.0080** 0.0084**

S.D. .00048 .00053 .00048 .00089

N 26 26 24 26

LIVER Ratio MEAN 0.0336 0.0339 0.0352** 0.0374**

S.D. .00159 .00147 .00255 .00188

N 26 26 24 26

LUNGS Ratio MEAN 0.0032 0.0032 0.0033 0.0032

S.D. .00039 .00026 .00031 .00032

N 26 26 24 26

PITUITARY Ratio MEAN 0.0000 0.0000 0.0000 0.0000

S.D. .00000 .00000 .00000 .00000

N 26 26 24 26

SPLEEN Ratio MEAN 0.0014 0.0013 0.0015 0.0014

S.D. .00019 .00023 .00018 .00016

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0111

- JAN

- 2010 13:21

Huntingd on Life Sciences 00

- 4205 PAGE 106

TA BLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO

FEMALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

FIN AL BODY WEIGHT g MEAN 308 312 310 311

S.D. 24.1 23.3 22.3 29.9

N 21 23 23 22

ADRENALS Ratio MEAN 0.0002 0.0002 0.0002 0.0002

S.D . .00002 .00004 .00005 .00003

N 21 23 23 22

BRAIN Ratio MEAN 0.0063 0.0062 0.0062 0.0063

S.D. .00053 .00045 .00040 .00050

N 21 23 23 22

KIDNEYS Ratio MEAN 0.0083 0.0083 0.0085 0.0087

S.D. .00044 .00050 .00055 .00050

N 21 23 23 22

LIVER Ratio MEAN 0.0451 0.0443 0.0463 0.0465

S.D. .00281 .00410 .00319 .00346

N 21 23 23 22

LUNGS Ratio MEAN 0.0047 0.0047 0.0048 0.0048

S.D. .00033 .00042 .00028 .00034

N 21 23 23 22

PITUITARY Ratio MEAN 0.0000 0.0001 0.0000 0.0000

S.D. .00001 .00001 .00001 .00001

N 21 23 23 22

SPLEEN Ratio MEAN 0.0019 0.0019 0.0019 0.0020

S.D. .00024 .00017 .00021 .00027

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 107

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BODY WEIGHT RATIO

FEMALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

OVARY RIGHT Ratio MEAN 0.0002 0.0002 0.0002 0.0002

S.D. .00004 .00004 .00003 .00004

N 21 23 23 22

OVARY LEFT Ratio MEAN 0.0002 0.0002 0.0002 0.0002

S.D. .00004 .00004 .00003 .00003

N 21 23 23 22

OVARIES Ratio MEAN 0.0004 0.0004 0.0004 0.0004

S.D. .00007 .00006 .00005 .00006

N 21 23 23 22

UTERUS/OVIDUCTS Ratio MEAN 0.0023 0.0023 0.0020 0.0022

S.D. .00062 .00063 .00054 .00058

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 108

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO

MALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

TESTIS

- RIGHT Ratio MEAN 0.7850 0.7751 0.8068 0.8298*

S.D. .05835 .04972 .06127 .06579

N 26 26 24 26

TESTIS

- LEFT Ratio MEAN 0.7854 0.7752 0.8112 0.8238

S.D. .06432 .04074 .06525 .06481

N 26 26 24 26

TESTES Ratio MEAN 1.5670 1.5453 1.6120 1.6487*

S.D. .12206 .08643 .12535 .12908

N 26 26 24 26

PROSTATE Ratio MEAN 0.5568 0.5291 0.5802 0.5618

S.D. .11538 .09237 .11568 .12730

N 26 26 24 26

SEM.VES & CO.GL. Ratio MEAN 0.8710 0.8640 0.9214 1.0247**

S.D. .16401 .17203 .15383 .14796

N 26 26 24 26

EPIDIDYMIS RIGHT Ratio MEAN 0.3252 0.3397 0.3478* 0.3518**

S.D. .02661 .028 77 .02472 .03174

N 26 25 24 26

EPIDIDYMIS LEFT Ratio MEAN 0.3231 0.3329 0.3462* 0.3449*

S.D. .02522 .02785 .02609 .03031

N 26 26 24 26

EPIDIDYMIDES Ratio MEAN 0.6433 0.6671 0.6904** 0.6928**

S.D. .04846 .04972 .04879 .05363

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Sta tistical key: * = p<0.05 ** = p<0.0111

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 109

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSAT E: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO

MALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS Ratio MEAN 0.0292 0.0298 0.0314 0.0285

S.D. .00428 .00540 .00544 .00471

N 26 26 24 26

KIDNEYS Ratio MEAN 1.8890 1.8772 2.1207** 2.2196**

S.D. .19685 .16948 .20311 .24610

N 26 26 24 26

LIVER Ratio MEAN 8.8903 9.0315 9.3552 9.9636**

S.D. .96476 1.0201 1.1247 1.1240

N 26 26 24 26

LUNGS Ratio MEAN 0.8482 0.8365 0.8599 0.8563

S.D. .10786 .07613 .08880 .06876

N 26 26 24 26

PITUITARY Ratio MEAN 0.0055 0.0053 0.0055 0.0055

S.D. .00062 . 00097 .00056 .00092

N 26 26 24 26

SPLEEN Ratio MEAN 0.3752 0.3556 0.3871 0.3712

S.D. .06294 .04879 .05626 .04767

N 26 26 24 26

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: ** = p<0.0111

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 110

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TO XICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO

FEMALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS Ratio MEAN 0.0379 0.0391 0.0378 0.0395

S.D. .00462 .00560 .00665 .00523

N 21 23 23 22

KIDNEYS Ratio MEAN 1.3246 1.3515 1.3695 1.3890

S.D. .11119 .09915 .09656 .11608

N 21 23 23 22

LIVER Ratio MEAN 7.1818 7.2160 7.4444 7.4674

S.D. .84545 .90048 .75483 .95689

N 21 23 23 22

LUNGS Ratio MEAN 0.7505 0.7588 0.7669 0.7670

S.D. .05244 .05567 .05428 .07663

N 21 23 23 22

PITUITARY Ratio MEAN 0.0074 0.0083 0.0075 0.0080

S.D. .00147 .00176 .00102 .00159

N 21 23 23 22

SPLEEN Ratio MEAN 0.3033 0.3123 0.3128 0.3170

S.D. .04462 .03379 .03959 .04714

N 21 23 23 22

OVARY RIGHT Ratio MEAN 0.0298 0.0286 0.0293 0.0301

S.D. .00632 .00632 .00452 .00654

N 21 23 23 22

OVARY LEFT Ratio MEAN 0.0273 0.0289 0.0276 0.0301

S.D. .00710 .00566 .00486 .0 0503

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 13:21

Huntingdon Life Sciences 00

- 4205 PAGE 111

TABLE 21 P0 GENERATION

GASOLINE DIPE VAPOR CON DENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF ORGAN WEIGHT TO BRAIN WEIGHT RATIO

FEMALES

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

OVARIES Ratio MEAN 0.0568 0.0572 0. 0569 0.0599

S.D. .01149 .00918 .00766 .00970

N 21 23 23 22

UTERUS/OVIDUCTS Ratio MEAN 0.3629 0.3680 0.3140 0.3471

S.D. .10607 .10587 .07336 .10174

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No stat istically significant differences

# Huntingdon Life Sciences 00

- 4205 Page 112

211

- DIPE

- 1G Final Report

Mean Pup Organ Weights

Preface Table 22

Table of Contents

F 1 

# Generation Absolute Organ Weights

........................................................... 113

F 1 

# Generation % Organ to Body Weight Ratios

................................................ 114

F 1 

# Generation % Organ to Brain Weight Ratios

................................................ 115

Corresponding exposure levels for each group were as follows:

Group I

- 0 mg/m 3



# Group II

- 2,000 mg/m 3



# Group III

- 10,000 mg/m 3



# Group IV

- 20,000 mg/m 311

- JAN

- 2010 13:28

Huntingdon Life Sciences 00

- 4205 PAGE 113

TABLE 22 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP ORGAN WEIGHT DATA

PUP ORGAN WEIGHTS IN GRAMS

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

BRAIN MEAN 1.5557 1.5503 1.5423 1.5353

S.D. .05 827 .05893 .07235 .04089

N 21 23 23 22

of Male Pups MEAN 1.5853 1.5684 1.5673 1.5608

S.D. .07752 .09306 .08504 .06183

N 21 23 23 22

of Female Pups MEAN 1.5262 1.5322 1.5172 1.5100

S.D. .05328 .05710 .07169 .03494

N 21 23 23 22

SPLEEN MEAN 0.3267 0.3108 0.3169 0 .2993

S.D. .05122 .04533 .05512 .04018

N 21 23 23 22

of Male Pups MEAN 0.3464 0.3232 0.3418 0.3115

S.D. .06832 .06291 .07812 .05702

N 21 23 23 22

of Female Pups MEAN 0.3070 0.2984 0.2920 0.2871

S.D. .05564 .03633 .04735 .04544

N 21 23 23 22

THYMUS MEAN 0.338 4 0.3726 0.3701 0.3511

S.D. .06328 .06684 .06418 .05561

N 21 23 23 22

of Male Pups MEAN 0.3378 0.3700 0.3671 0.3440

S.D. .08071 .07689 .09186 .08622

N 21 23 23 22

of Female Pups MEAN 0.3389 0.3753 0.3731 0.3582

S.D. .06696 .07188 .05396 .04127

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statistically significant differences11

- JAN

- 2010 13:28

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TABLE 22 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP ORGAN WEIGHT DATA

PUP ORGAN WEIGHT TO BODY WEIGHT RATIO

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

BRAIN MEAN 2.0057 2.0114 2.0624 2.0439

S.D. .18098 .21163 .238 22 .14768

N 21 23 23 22

of Male Pups MEAN 1.9699 1.9982 2.0421 2.0405

S.D. .20062 .27934 .35601 .20930

N 21 23 23 22

of Female Pups MEAN 2.0415 2.0246 2.0826 2.0473

S.D. .19226 .18312 .17288 .14212

N 21 23 23 22

SPLEEN MEAN 0.4148 0.3986 0.4143 0.3949

S.D. .03911 .03784 .04467 .04883

N 21 23 23 22

of Male Pups MEAN 0. 4234 0.4055 0.4315 0.4021

S.D. .04498 .05447 .05852 .05190

N 21 23 23 22

of Female Pups MEAN 0.4062 0.3917 0.3971 0.3876

S.D. .05675 .03284 .04849 .05680

N 21 23 23 22

THYMUS MEAN 0.4314 0.4766* 0.4841 ** 0.4631

S.D. .06560 .05889 .04908 .05711

N 21 23 23 22

of Male Pups MEAN 0.4140 0.4631 0.4602 0.4422

S.D. .07853 .06406 .07189 .07941

N 21 23 23 22

of Female Pups MEAN 0.4487 0.4902 0.5080** 0.4840

S.D. .07897 .06840 .04737 .04990

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

Statistical key: * = p<0.05 ** = p<0.0111

- JAN

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T ABLE 22 F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF PUP ORGAN WEIGHT DATA

PUP ORGAN WEIGHT TO BRAIN WEIGHT RATIO

-----------------------------------------------------------------------------------------------------------------------------------

DOSE GROUP I II III IV

DOSE LEVEL (MG/M3) 0 2000 10000 20000

-----------------------------------------------------------------------------------------------------------------------------------

SP LEEN MEAN 20.926 20.019 20.477 19.496

S.D. 2.8883 2.6336 3.3055 2.6867

N 21 23 23 22

of Male Pups MEAN 21.780 20.568 21.717 19.976

S. D. 3.7017 3.5423 4.4926 3.6668

N 21 23 23 22

of Female Pups MEAN 20.071 19.469 19.236 19.015

S.D. 3.3357 2.1427 3.0478 3.0321

N 21 23 23 22

THYMUS MEAN 21.702 23.991 23.922 22.881

S.D. 3.6459 3.9452 3.7068 3.4589

N 21 23 23 22

of Male Pups MEAN 21.231 23.537 23.2 91 22.044

S.D. 4.6190 4.3796 5.3658 5.4792

N 21 23 23 22

of Female Pups MEAN 22.174 24.446 24.553 23.718

S.D. 4.2181 4.3078 3.0137 2.6346

N 21 23 23 22

-----------------------------------------------------------------------------------------------------------------------------------

No statis tically significant differences

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Lesion Incidence Summary

with Expanded Severity Levels

Preface Table 23

Key to Abbreviations

Gl = Gland

NAD = No abnormal diagnoses

Ctls = Controls (Group 1)

Oviducts/Fallop = Oviducts and Fallopian Tubes

Corresponding exposure levels for each group were as follows:

Group 1

- 0 mg/m 3



# Group 2

- 2,000 mg/m 3



# Group 3

- 10,000 m g/m 3



# Group 4

- 20,000 mg/m 3Page 117Page 118Page 119Page 120

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Inhalation Report Appendix A

TABLE OF CONTENTS

1. INTRODUCTION ..............................................................................................122

2. MATERIALS AND METHODS

2.1. Husbandry During Exposure Periods .......................................................122

2.2. Test Substance Administration and Chamber Operation .........................123

2.3. Exposure Concentration Determination ...................................................127

3. TABLES

I. Summary of In -Chamber Observations ...................................................129

II. Chamber Monitoring Records ..................................................................137

III. Equ ipment List .........................................................................................162

IV. Chamber Distribution Results ..................................................................165

V. Miran Calibration .....................................................................................167

VI. Testing Room and Chambers Environmental Monitoring .......................170

4. FIGURES

I. Chamber Generation System and Whole-Body Exposure

Chamber – Group I ..............................................................................171

II. Chamber Generation System and Whole-Body Exposur e

Chamber

– Groups II, III, IV ...............................................................172

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Inhalation Report Appendix A

1. INTRODUCTION

This appendix presents the methodology for exposure atmosphere generation

monitoring and results.

2. MATERIALS AND METHOD S

2.1. HUSBANDRY DURING EXPOSURE PERIODS

2.1.1. HOUSING

Animals were individually housed in stainless steel, wire mesh

cages within a 1.5 m 3 

# glass and stainless steel whole -body

exposure chamber. The placement of the animals in the whole

body exposure chamber was rotated weekly to ensure uniform

exposure of the animals. A description of the animal rotation is

included in the raw data.

2.1.2. FEE D

None was provided during exposure.

2.1.3. WATER

None was provided during exposure.

2.1.4. ENVIRONMENTAL CONDITIONS

Chamber temperature and relative humidity were recorded every

half

- hour during exposure and maintained, to the maximum extent

possible, within the ranges presented below. Excursions outside

the specified range did not affect the integrity of the study.

Temperature

Desired: 20 to 24 ° C

Actual: 21 to 28 ° C

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The daily average temperature within the chamber was outside the

desired range on 10/125 days , 105/126 days

, 106/125 days and

103/127 days for Groups 1, 2, 3 and 4, respectively.

Relative Humidity

Desired: 40 to 60%

Actual: 35 to 65%

The daily average relative humidity within the chamber was

outside the desired range on 3/125 days

, 10/126 days

, 6/125 days

and 4/127 days for Groups 1, 2, 3 and 4, respectively.

2.2. TEST SUBSTANCE ADMINISTRATION AND CHAMBE R

OPERATIONS

2.2.1. ROUTE OF ADMINISTRAT ION

Inhalation via whole-body exposures

2.2.2. TEST SUBSTANCE ADMINISTRATION

The test substance was administered as a vapor in the bre athing air

of the animals. The test atmosphere was generated by an

appropriate procedure determined during the pre

- study trials. The

trials were performed to evaluate the optimal set of conditions and

equipment to generate a stable atmosphere at the target exposure

levels and maintain uniform conditions throughout the exposure

chambers.

2.2.3. JUSTIFICATION FOR ROUTE OF ADMINISTRATIO N

The inhalation route is one of the potential routes of human

exposure to this test substance and is the route specified in the

re ferenced US EPA 79.61 guidelines.

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2.2.4. TARGET EXPOSURE LEVELS

Group I - 0 mg/m 3



# Group II - 2,000 mg/m 3



# Group III - 10,000 mg/m 3



# Group IV - 20,000 mg/m 3



# 2.2.5. JUSTIFICATION FOR EX POSURE LEVEL SELECTI ON

Exposure levels were selected by the Sponsor, based on results

fro m a 13

- week inhalation study conducted at the Testing Facility

that utilized this test substance in rats (00

- 6130). The exposure

levels were also selected based on the lower flammable limits of

the test substance.

2.2.6. FREQUENCY OF ADMINISTRATION

Frequency

P 0 

# males and females received 70 consecutive days (ten weeks) of

exposure prior to mating for six hours/day. P 0 

# males and females

continued to be exposed daily throughout a 14

- day cohabitation

period. The mated females continued to be exposed daily from

Day 0 through 19 of gestation. Beginning on Day 5 of lactation,

nursing P 0 

# females were exposed daily until weaning of the F 1 

# offspring on Day 28. P 0 

# females with no confirmed day of mating

but with evidence of pregnancy continued exposure until presumed

Day 19 of gestation. P 0 

# females with no confirmed day of mating

and with no evidence of pregnancy (weight gain) continued

exposure for 25 days following completion of the mating period

and then euthanized. P 0 

# females with a confirmed day of mating

that did not deliver were euthanized on presumed Day 25 of

gestation.

P 0 

# males were exposed daily until euthanasia, which occurred after

the date that the last F 1 

# litters were delivered.

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2.2.7. DURATION

The test substance was administered for 6 hours/day during all

segment s of the study.

2.2.8. EXPOSURE PROCEDURE AND CHAMBER OPERATION S

Group I

Houseline nitrogen was delivered from a regulator with a

backpressure gauge via 1⁄4” tubing to a flowmeter regulated by a

metering valve. This nitrogen flow (~ 20 Lpm) was then directed

into the turret of the 1.5 m 3 

# glass and stainless steel exposure

chamber where it was mixed with room air as it was drawn into the

chamber. This nitrogen flow simulated the generation nitrogen

flow for Groups II, III, and IV.

Groups II, III and IV

Houseline ni trogen was delivered from a regulator with a

backpressure gauge through a stainless steel fitting to create three

flow systems: the test substance pressurization flow, the purge flow

and the volatilization flow.

The nitrogen for the test substance pressuri zation flow was directed

via 1⁄4” tubing through a metering valve, attached to a backpressure

gauge, into the vapor inlet valve of the test substance cylinder. The

metering valve was used to adjust and maintain the pressure within

the cylinder. From the pr essurized cylinder, the test substance

flowed from the liquid outlet valve through a quick -disconnect

fitting and through a filter to prevent equipment contamination.

From the filter, the test substance flowed to a liquid flowmeter via

1 

# / 8 

# ” tubing. The o utlet of the flowmeter was regulated by a built -in

metering valve. From this metering valve, the test substance

flowed via 1 

# / 8 

# ” tubing onto the glass helix of a counter current

volatilization chamber. The glass helix was heated by a nichrome

wire, which was controlled by an autotransformer and was inserted

in the center of the glass tube that supported the helix.

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The nitrogen for the purge flow system was directed, via 1⁄4” tubing

to a flowmeter regulated by a metering valve. The purge nitrogen

was delivered via 1 

# / 8 

# ” tubing to the bottom of the tube containing

the nichrome wire. This nitrogen flow continuously purged the

area surrounding the nichrome wire within the tube, thereby

protecting the wire from oxidation.

The nitrogen for the volatilization syste m was directed via 1⁄4”

tubing to a flowmeter regulated by a metering valve. From the

flowmeter, the volatilization nitrogen (~ 20 Lpm) flowed via 1⁄4”

tubing to a ball and socket joint at the bottom of the volatilization

chamber. This nitrogen flowed up thr ough the volatilization

chamber passing over the coil and volatilizing the test substance.

The pressure within the counter

- current volatilization chamber was

maintained slightly negative to the room and was monitored with a

pressure gauge.

This test subst ance laden nitrogen exited the top of the

volatilization chamber through a glass elbow, which directed the

flow, via 1⁄2” tubing, to the turret of 1.5 m 3 

# glass and stainless steel

exposure chamber. As the test substance laden nitrogen was drawn

into the cha mber, it was mixed with room air.

The whole

- body exposure chambers each had a volume of

approximately 1500 Liters (1.5 m 3 

# ). Each chamber was operated at

a minimum flow rate of 300 Liters per minute. The final airflow

was set to provide at least one air c hange (calculated by dividing

the chamber volume by the airflow rate) in 5.0 minutes (12 air

changes/hour) and a T 99 

# equilibrium time (calculated by

multiplying the air change by the exponential factor 4.6) of at most

23 minutes:

Group Airflow Rate Air Cha nge T 99 

# (Lpm) (min) (min)

I 314 4.8 22

II 303 5.0 23

III 303 5.0 23

IV 312 4.8 22

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This chamber size and airflow rate was considered adequate to

maintain the oxygen level at least 19% and the animal -loading

factor below 5%. Chamber temperature, humid ity, airflow rate and

static pressure were monitored continuously and recorded every

30 minutes during exposure. At the end of the 6

- hour exposure, all

animals remained in the chamber for a minimum of 30 minutes.

During this time the chamber was operated at approximately the

same flow rate using clean air only. The chambers were exhausted

through the in house filtering system, which consisted of a coarse

filter, a HEPA filter and activated charcoal.

See Chamber Figures I and II and Table III for equipment details.

2.3. EXPOSURE CONCENTRATION DETERMINATION

2.3.1. NOMINAL CONCENTRATIO N

A nominal exposure concentration was calculated. The flow of air

through the chamber was monitored using appropriate calibrated

equipment. The test substance consumed (weight difference of the

test substance cylinder) during the exposure (mg) was divided by

the total volume of air (m 3 

# ) passing through the chamber

(volumetric flow rate times total exposure time) to give the

nominal concentration.

2.3.2. CHAMBER SAMPLING

During each exposure, measurements of airborne concentrations

were performed in the animals’ breathing zone at least 4 times

using an appropriate sampling procedure and Infra

- red

Spectrophotometric (IR) analytical method. Also, one charcoal

tube sample per chamber per week was analyzed by gas

chromatography (GC) to characterize at least 10 major components

(comprising at least 80% by weight of the test substance) to show

test substance stability and comparison between the neat liquid test

substance and the vaporized test atmospher es.

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Inhalation Report Appendix A

See Table III for equipment details. See Appendix B for Analytical

Report.

2.3.3. PARTICLE SIZE DISTRIBUTION

During each week of exposure, particle size determinations were

performed using a TSI Aerodynamic Particle Sizer to characterize

the aerodynamic p article size distribution of any aerosol present.

See Table III for equipment details.

2.3.4. CHAMBER AND EXPOSURE ROOM ENVIRONMENT

Chamber oxygen levels (maintained at least 19%) were measured

pretest and at the beginning, middle, and end of the study.

Air sa mples were taken in the vapor generation area pretest and at

the beginning, middle and end of the study. Light (maintained

approximately 30 foot

- candles at 1.0 meters above the floor) and

noise levels (maintained below 85 decibels) in the exposure room

we re measured pretest and at the beginning, middle and end of the

study.

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32

Group I

– 0 mg/m 3



## Normal

Within Normal Limi ts All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

# Huntingdon Life Sciences 00

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Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## No rmal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All A ll All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure D ay 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Inhalation Report Appendix A

Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All A ll All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 m g/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg/m 3



## Normal

Withi n Normal Limits All All All All All All All All All All All All All All All All

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Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group II

– 2,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

Group IV

– 20,000 mg /m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All All All

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Table I

Summary of In

- Chamber Observations

All = 100% of the animals exhibiting a given observation.

Note: In

- chamber observations are based on all animals present in the exposure chamber at the time.



## Exposure Day 113 114 115 116 117 118 119 120 121 122 123 124 125 126

Group I

– 0 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All

- -

Group II

– 2,000 mg/m 3



## Normal

W ithin Normal Limits All All All All All All All All All All All All All

-

Group III

– 10,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All

- -

Group IV

– 20,000 mg/m 3



## Normal

Within Normal Limits All All All All All All All All All All All All All All

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Table II

Chamber Monitoring Records

Preface

Key to Abbreviations

MMAD = Mass Median Aerodynamic Diameter

GSD = Geometric Standard Deviation

TMC = Total Mass Concentration

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Table III

Equipment List

Exposure Chamber

1500 Liter glass and stainless steel chamber (Unifab Cages).

Chamber Air

- flow Gauges

Dwyer ® 

# Magnehelic ® 

# gauge (Dwyer  Instruments Inc.), calibrated prestudy with

a Side Trak  III, equipped with a Digital Meter, Model 831

- N2 (Sierra

Instru ments, Inc.).

Chamber Static Pressure

Dwyer ® 

# Magnehelic ® 

# gauge (Dwyer ® 

# Instruments Inc.); calibrated prestudy with a

Dwyer  

# Mark II Manometer, Model 25 (Dwyer  

# Instruments Inc.).

Compound Generator

Counter

- Current Volatilization Unit, coiled glass rod insert with nichrome wire

(Crown Glass Co., Inc.).

Flowmeter with built

- in valve, size 0

- 65 mm, tube #’s 6G02R3, 6G03R3, 6G04R3

(Key Instruments).

Variable Autotransformer, Type 3PN 1010 (Staco Energy Products Company).

T ° Sentry Digital Alarm Module, Model 110 (Hampshire Controls Corp.).

Balston ® 

# Microfibre  Disposable Filter Units, No. L9933

- 05 (Grade DQ).

Quick

- disconnect fitting with toggle valve (Rego  

# ).

Compound Reservoir

5- gallon cylinder (Manchester Tank).

Balance

Mettler PM30000K (Mettler Instrument Corporation).

Pelooze, Model No. 4010.

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Table III

Equipment List

Flowmeters

Flowmeter, size 0

- 4, 0

- 10, 0

- 20, 0

- 40 Lpm (Dwyer ® 

# Instruments Inc.).

Top Trak  Mass Flow Meter size 0

- 1 Lpm, Model 821

- 1 (Sierra Instruments),

calibrated prestudy with a Gilibrator  

# Bubble Generator, P/N D800286, S/N

569

- S, flow cell assembly P/N D800268, BD #1860).

Pressure/Vacuum Gauges

Ashcroft ® 

# backpressure gauge, P/N 733

- 47.

GAST ® 

# vacuum gauge, Model 169

- 01.

Matheson ® 

# backpressure gauge, P/N 63

- 3161.

Norgreen backpressure gauge, P/N 9892K23.

Dw yer ® 

# Magnehelic ® 

# gauge (Dwyer ® 

# Instruments Inc.).

Regulator

Norgreen, P/N 9892K23.

Valves

Metering Valve, Model SS

- 4L Series (Nupro ® 

# Co.).

Tubing

Plastic Size 1/2”, 1/4”, 3/16” (Norton); 1/8” (Tygon).

Teflon ® 

# , size 1/8”, 1/4”, 1/2”.

Stainless steel 1/2”

. T- Tube, stainless steel.

Glass elbow tube (Crown Glass Co.).

Stainless steel cross (Swage).

Filter

Balston ® 

# Microfibre  Disposable Filter Units, Grade DQ.

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Table III

Equipment List

Air Analyzer

MIRAN ® 

# 1A

- CVF Ambient Air Analyzer (Wilks) with a Cole Parmer strip recorder

Model 201 and a Micronta ® 

# LCD Benchtop Digital Multimeter No. 22

- 195.

Oxygen/Gas Analyzer, Model 12145 (Gastech).

Digital Sound Level Meter, Model 840029 (Speer Scientific).

Photometer/Light Meter (Quantum Instruments).

Particle Sizer/Analyzer

TSI Aerodynamic P article Sizer, Model 331001 and a DELL computer, Model

486P/25, equipped with an Epson LQ

- 570+ Dot matrix printer, Model P630B.

Absorbent Tube Sampling

Charcoal Tubes, ORBO

- 32, Lot #2000 (Supelco).

Vacuum Pumps

Thomas Industries Inc., Model 707CM50, 107CA18 3.

Timer

Gralab Universal Timer, Model 171.

Environmental Monitoring

VWR Big Digit Temperature and Humidity Gauge, tested prestudy with a VWR

NIST Traceable Digital Hygrometer/Thermometer.

# Huntingdon Life Sciences 00

- 4205 P age 165

211

- DIPE

- 1G Final Report

Inhalation Report Appendix A

Table IV

Chamber Distribution Records

Group

(target) Date Port IR Conc

(mg/m 3 

# ) Ratio to H

- 3

II (2,000 mg/m 3 

# ) 17

- May

- 02 H

- 3 1930 1.00

H

- 9 1910 0.99

H

- 3 1950 1.00

H

- 1 1890 0.97

H

- 2 1940 0.99

H

- 3 2080 1.00

H

- 8 2020 0.97

H

- 7 2100 1.01

18

- May

- 02 H

- 3 2040 1.00

H

- 14 2090 1. 02

H

- 13 1950 0.96

H

- 12 1870 0.92

H

- 11 1880 0.92

III (10,000 mg/m 3 

# ) 17

- May

- 02 H

- 3 10300 1.00

H

- 1 8390 0.81

H

- 2 9680 0.94

H

- 8 10200 0.99

H

- 3 9870 1.00

H

- 9 9680 0.98

H

- 7 10000 1.01

18

- May

- 02 H

- 3 9940 1.00

H

- 14 10100 1.02

H

- 13 9610 0.97

H

- 12 9840 0.99

H

- 3 10700 1.00

H

- 11 10200 0.95

IV (20,000 mg/m 3 

# ) 17

- May

- 02 H

- 3 19300 1.00

H

- 9 19000 0.98

H

- 3 20300 1.05

H

- 1 20400 1.06

H

- 2 20200 1.05

H

- 7 20400 1.06

# Huntingdon Life Sciences 00

- 4205 P age 166

211

- DIPE

- 1G Final Report

Inhalation Report Appendix A

Table IV

Chamber Distribution Records

Group

(target) Date Port IR Conc

(m g/m 3 

# ) Ratio to H

- 3

IV (20,000 mg/m 3 

# ) 17

- May

- 02 H

- 3 20300 1.00

H

- 8 20100 0.99

18

- May

- 02 H

- 3 20100 1.00

H

- 14 20200 1.00

H

- 13 20300 1.01

H

- 12 20400 1.02

H

- 3 19900 1.00

H

- 11 20000 1.01

# Huntingdon Life Sciences 00

- 4205 Page 167

211

- DIPE

- 1G Final Report

Inhalation Report Appendix A

Table V

Miran Calibration

Methodology for Gasoline DIPE Vapor Condensate

Settings: The instrument settings for the unit are summarized below:

wavelength, microns 10.2

pathlength, dial setting 5.30

slit width, mm 1

range, absorbance 1

response, seconds 1

gain High

chart speed, cm/min 1

chart volts 1

Calibrations: The Miran ® 

## was turned on and allowed to warm up for approximately

10 minutes. The cell was flushed with room air for approximately one minute. The loop

was closed, the unit was zeroed and the calibration se ries was performed as shown

below. The resultant data were plotted to obtain a calibration curve. Each observer

used a separate syringe for calibration.

Injection Calculated Absorbance 2



## Volume Concentration 1 

## Operator 1 Operator 2 Average

(μL) (m g/m 3 

## ) (volts) (volts) (volts)

8.4 953 0.0333 0.0325

0.0313 0.0304 0.0319

17 1929 0.0684 0.0711

0.0675 0.0698 0.0692

84 9532 0.319 0.321

0.315 0.316 0.318

170 19291 0.648 0.580

0.618 0.640 0.6 22

210 23830 0.788 0.838

0.770 0.760 0.7 89

1 

## Calculated Conc. (mg/m 3 

## ) = Injection volume (μL) x Density (mg/μL) x 1000 L/m 3



## 5.64 L

where density = 0.6 4 mg/μL and 5.64 L = volume of Miran cell

2 

## Due to the variability of the initial results, two additional injections (one per operator) were

performed for each concentration. All four values per concentration were used for the regression

analysis.

# Huntingdon Life Sciences 00

- 4205 Page 168

211

- DIPE

- 1G Final Report

Inhalation Report Appendix A

Table V

Miran Calibration

Calibration Checks: A three

- point calibration check of the Miran ® 

# was

performed for each exposure prior to sampling the chambers. The parameters

are shown below:

Expected Acceptable

Injection Calculated Absorbance Absorbance

Volume Concentration Reading Range

(μL) (mg/m 3 

# ) (volts) (volts)

17 1929 0.0692 0.0588

– 0.0796

84 9532 0.318 0.2703

– 0.366

170 19291 0.622 0.529

– 0.715

The absorbance was recorded after each injection. The absorbance was

considered satisfactory if it was within 1 5% of the original calibration series. If

any of the absorbance values fell outside the 15% range, the injection was

rechecked as follows. The volume for the value that was out of range was

reinjected twice. The closer pair of the three injections were averaged and the

results were compared to the original curve. If the average of the pair was within

the 15% range, the original was accepted. If the value of the average was

outside the 15% range, the Study Director decided if a new graph was to be

prepared.

# Huntingdon Life Sciences 00

- 4205 Page 169

211

- DIPE

- 1G Final Report

Inhalation Report Appendix A

Table V

Miran Calibration

Calibration Curve for Gasoline DIPE Vapor Condensate

y = -40124x 4 

## + 41398x 3 

## - 6533.9x 2 

## + 29104x

R 2 

## = 0.9977

0 5000 10000 15000 20000 25000 30000

0.0000 0.2000 0.4000 0.6000 0.8000 1.0000

Absorbance (Volts)

# Huntingdon Life Sciences 00

- 4205 Page 170

211

- DIPE

- 1G Final Report

Appendix A

Table VI

Testing Room and Chambers Environmental Monitoring

Interval Location Testing

Room

Test Substance

(mg/m 3 

# ) Testing

Room

Light

(Ft Candles) Testing

Room

Noise

(dB) Chamber

Oxygen

(%)

Pretest Room 808 0 39.4 60.3

- Group I

- - - 20

Group II

- - - 20

Group III

- - - 20

Group IV

- - - 20

Week 0 Room 808 0 35.0 62.5

- Group I

- - - 20

Group II

- - - 20

Group III

- - - 20

Group IV

- - - 20

Week 9 Room 808 0 38.1 61.1

- Group I

- - - 20

Group II

- - - 20

Group III

- - - 20

Group IV

- - - 20

Week 18 Roo m 808 0 37.3 64.2

- Group I

- - - 20

Group II

- - - 20

Group III

- - - 20

Group IV

- - - 20

# Huntingdon Life Sciences 00

- 4205 Page 171

211

- DIPE

- 1G Final Report

Group I Inhalation Report Appendix A

Figure I

Chamber Generation System

and Whole

- Body Exposure Chamber

Notes:

1. Sampling Ports H

- 11 (left

- bottom), H

- 12 (left

- top), H

- 13 (right

- bottom) and

H- 14 (right

- top) used for pretest distribution sampling, were located on the

back wall of the chambers.

2. Animals where individually housed on five levels within the exposu re

chamber.

# Huntingdon Life Sciences 00 -4 205 Page 172

211

- DIPE

- 1G Final Report

Groups II, III

and IV Inhalation Report Appendix A

Figure II

Chamber Generation System

and Whole

- Body Exposure Chamber

Notes:

1. Sampling Ports H

- 11 (left

- bottom), H

- 12 (left

- top), H

- 13 (right

- bottom) and

H- 14 (right

- top) used for pretest distribution sampling, were located on the

back wall of the chambers.

2. Animals where individually housed on five levels within the exposure

chamber.11

- JAN

- 2010 16:15

Huntingdon Life Sciences 00

- 4205 PAGE 203

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

1026 TERMINAL SACRIFICE 10

- SEP

- 02 113

1027 TERMINAL SACRIFICE 10

- SEP

- 02 113

1028 TERMINAL SACRIFICE 10

- SEP

- 02 113

1029 TERMINAL SACRIFICE 10

- SEP

- 02 113

1030 TERMINAL SAC RIFICE 10

- SEP

- 02 113

1031 TERMINAL SACRIFICE 10

- SEP

- 02 113

1032 TERMINAL SACRIFICE 10

- SEP

- 02 113

1033 TERMINAL SACRIFICE 10

- SEP

- 02 113

1034 TERMINAL SACRIFICE 10

- SEP

- 02 113

1035 TERMINAL SACRIFICE 10

- SEP

- 02 113

1036 TERMINAL SACRIFICE 10

- SEP

- 02 113

1037 TERMINAL SACRIFICE 10

- SEP

- 02 113

1038 TERMINAL SACRIFICE 10

- SEP

- 02 113

1039 TERMINAL SACRIFICE 11

- SEP

- 02 114

1040 TERMINAL SACRIFICE 11

- SEP

- 02 114

1041 TERMINAL SACRIFICE 11

- SEP

- 02 114

1042 TERMINAL SACRIFICE 11

- SEP

- 02 114

1043 TERMINAL SACRIFICE 11

- SEP

- 02 114

1044 TERMINAL SACRIFICE 11

- SEP

- 02 114

1045 TERMINAL SACRIFICE 11

- SEP

- 02 114

1046 TERMINAL SACRIFICE 11

- SEP

- 02 114

1047 TERMINAL SACRIFICE 11

- SEP

- 02 114

1048 TERMINAL SACRIFICE 11

- SEP

- 02 114

1049 TERMINAL SACRIFICE 11

- SEP

- 02 114

1050 TERMINAL SACRIFICE 1 1- SEP

- 02 114

1051 TERMINAL SACRIFICE 11

- SEP

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 16:1 5

Huntingdon Life Sciences 00

- 4205 PAGE 204

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

2026 TERMINAL SACRIFICE 10

- SEP

- 02 113

2027 TERMINAL SACRIFICE 10

- SEP

- 02 113

2028 TERMINAL SACRIFICE 10

- SEP

- 02 113

2029 TERMINAL SACRIFICE 10

- SEP

- 02 113

2030 TERMINAL SACRIFICE 1 0- SEP

- 02 113

2031 TERMINAL SACRIFICE 10

- SEP

- 02 113

2032 TERMINAL SACRIFICE 10

- SEP

- 02 113

2033 TERMINAL SACRIFICE 10

- SEP

- 02 113

2034 TERMINAL SACRIFICE 10

- SEP

- 02 113

2035 TERMINAL SACRIFICE 10

- SEP

- 02 113

2036 TERMINAL SACRIFICE 10

- SEP

- 02 113

2037 TERMINAL SACRIFICE 10

- SEP

- 02 113

2038 TERMINAL SACRIFICE 10

- SEP

- 02 113

2039 TERMINAL SACRIFICE 11

- SEP

- 02 114

2040 TERMINAL SACRIFICE 11

- SEP

- 02 114

2041 TERMINAL SACRIFICE 11

- SEP

- 02 114

2042 TERMINAL SACRIFICE 11

- SEP

- 02 114

2043 TERMINAL SACRIFICE 11

- SEP

- 02 114

20 44 TERMINAL SACRIFICE 11

- SEP

- 02 114

2045 TERMINAL SACRIFICE 11

- SEP

- 02 114

2046 TERMINAL SACRIFICE 11

- SEP

- 02 114

2047 TERMINAL SACRIFI CE 11

- SEP

- 02 114

2048 TERMINAL SACRIFICE 11

- SEP

- 02 114

2049 TERMINAL SACRIFICE 11

- SEP

- 02 114

2050 TERMINAL SACRIFICE 11

- SEP

- 02 1 14

2051 TERMINAL SACRIFICE 11

- SEP

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 16:15

Huntingdon Life Sciences 00

- 4205 PAGE 205

APPEN DIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

3026 TERMINAL SACRIFICE 10

- SEP

- 02 113

3027 TERMINAL SACRIFI CE 10

- SEP

- 02 113

3028 TERMINAL SACRIFICE 10

- SEP

- 02 113

3029 TERMINAL SACRIFICE 10

- SEP

- 02 113

3030 TERMINAL SACRIFICE 10

- SEP

- 02 1 13

3031 TERMINAL SACRIFICE 10

- SEP

- 02 113

3032 TERMINAL SACRIFICE 10

- SEP

- 02 113

3033 TERMINAL SACRIFICE 10

- SEP

- 02 113

3034 TERMINAL SACRIFICE 10

- SEP

- 02 113

3035 TERMINAL SACRIFICE 10

- SEP

- 02 113

3036 TERMINAL SACRIFICE 10

- SEP

- 02 113

3037 TERMINAL SACRIFICE 10

- SEP

- 02 113

3038 TERMINAL SACRIFICE 10

- SEP

- 02 113

3039 TERMINAL SACRIFICE 11

- SEP

- 02 114

3040 TERMINAL SACRIFICE 11

- SEP

- 02 114

3041 TERMINAL SACRIFICE 11

- SEP

- 02 114

3042 TERMINAL SACRIFICE 11

- SEP

- 02 114

3043 HS HUMANE SACRIFICE 12

- JUN

- 02 23

3044 DIED FOUN D DEAD 7

- SEP

- 02 110

3045 TERMINAL SACRIFICE 11

- SEP

- 02 114

3046 TERMINAL SACRIFICE 11

- SEP

- 02 114

3047 TERMINAL SACRIFICE 11

- SE P- 02 114

3048 TERMINAL SACRIFICE 11

- SEP

- 02 114

3049 TERMINAL SACRIFICE 11

- SEP

- 02 114

3050 TERMINAL SACRIFICE 11

- SEP

- 02 114

3051 TERMINAL SACRIFICE 11

- SEP

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD HS=HUMANE SACRIFICE11

- JAN

- 2010 16:15

Huntingdon Life Sciences 00

- 4205 PAGE 206

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY

ANIMAL# DEATH DEATH DAY

-----------------------------------------------------------------------------------------------------------------------------------

4026 TERMINAL SACRIFICE 10

- SEP

- 02 113

4027 TERMINAL SACRIFICE 10

- SEP

- 02 113

4028 TERMINAL SACRIFICE 10

- SEP

- 02 113

4029 TER MINAL SACRIFICE 10

- SEP

- 02 113

4030 TERMINAL SACRIFICE 10

- SEP

- 02 113

4031 TERMINAL SACRIFICE 10

- SEP

- 02 113

4032 TERMINAL SACRIFICE 10 -S EP

- 02 113

4033 TERMINAL SACRIFICE 10

- SEP

- 02 113

4034 TERMINAL SACRIFICE 10

- SEP

- 02 113

4035 TERMINAL SACRIFICE 10

- SEP

- 02 113

4036 TERMINAL SACRIFICE 10

- SEP

- 02 113

4037 TERMINAL SACRIFICE 10

- SEP

- 02 113

4038 TERMINAL SACRIFICE 10

- SEP

- 02 113

4039 TERMINAL SA CRIFICE 11

- SEP

- 02 114

4040 TERMINAL SACRIFICE 11

- SEP

- 02 114

4041 TERMINAL SACRIFICE 11

- SEP

- 02 114

4042 TERMINAL SACRIFICE 11

- SEP

- 02 114

4043 TERMINAL SACRIFICE 11

- SEP

- 02 114

4044 TERMINAL SACRIFICE 11

- SEP

- 02 114

4045 TERMINAL SACRIFICE 11

- SEP

- 02 114

4046 TERMINAL SACRIFICE 11

- SEP

- 02 114

4047 TERMINAL SACRIFICE 11

- SEP

- 02 114

4048 TERMINAL SACRIFICE 11

- SEP

- 02 114

4049 TERMINAL SACRIFICE 11

- SEP

- 02 114

4050 TERMINAL SACRIFICE 11

- SEP

- 02 114

4051 TERMINAL SACRIFICE 11

- SEP

- 02 114

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 16:15

Huntingdon Life Sciences 00

- 4205 PAGE 207

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

1526 TERMINAL SACRIFICE 21

- SEP

- 02 124 P

1527 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1528 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1529 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

1530 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

1531 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

1532 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

1533 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1534 TERMINAL SACRIFICE 23

- AUG

- 02 95 NP

1535 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1536 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

1537 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

1538 TERMINAL SACRIFICE 26

- AUG

- 02 98 NP

1539 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1540 NNMD TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

1541 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1542 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1543 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

1544 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

1545 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1546 TERMINA L SACRIFICE 17

- SEP

- 02 120 P

1547 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1548 TERMINAL SACRIFICE 24

- AUG

- 02 96 NP

1549 TLL TERMINAL SACRIFICE 22

- SEP -0 2 125 P

1550 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

1551 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS11

- JAN

- 2010 16:15

Huntingdon Life Sci ences 00

- 4205 PAGE 208

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATU S

-----------------------------------------------------------------------------------------------------------------------------------

2526 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2527 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2528 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

2529 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

2530 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

2531 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2532 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2533 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2534 TERMI NAL SACRIFICE 18

- SEP

- 02 121 P

2535 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

2536 HS HUMANE SACRIFICE 6

- AUG

- 02 78 P

2537 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

2538 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

2539 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2540 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

2541 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

2542 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

2543 PNMD TERMINAL SACRIFICE 23

- SEP

- 02 126 P

2544 NNMD TERMINAL SACR IFICE 5

- SEP

- 02 108 NP

2545 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

2546 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

2547 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

2548 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

2549 NNMD TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

2550 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

2551 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE11

- JAN

- 2010 16:15

Huntingdon Life Sciences 00

- 4205 PAGE 209

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP II I 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

3526 NNMD TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

3527 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

3528 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

3529 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

3530 NNMD TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

3531 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

3532 TERMINAL SA CRIFICE 19

- SEP

- 02 122 P

3533 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

3534 TLL TERMINAL SACRIFICE 22

- SEP

- 02 125 P

3535 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

3536 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

3537 TERMINAL SACRIFICE 22

- SEP

- 02 125 P

3538 TERMINAL SACRIFICE 21

- SEP

- 02 124 P

3539 TERMINAL SACRIFICE 21

- SEP

- 02 124 P

3540 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

3541 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

3542 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

3543 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

3544 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

3545 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

3546 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

3547 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

3548 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

3549 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

3550 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

3551 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS11

- JA N- 2010 16:15

Huntingdon Life Sciences 00

- 4205 PAGE 210

APPENDIX C P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ANIMAL TERMINATION HISTORY

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TYPE OF DATE OF STUDY PREGNANCY

ANIMAL# DEATH DEATH DAY STATUS

-----------------------------------------------------------------------------------------------------------------------------------

4526 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

452 7 TERMINAL SACRIFICE 24

- SEP

- 02 127 P

4528 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

4529 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

4530 PNMD TERMINAL SACRIFIC E 19

- SEP

- 02 122 P

4531 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

4532 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

4533 TERMINAL SACRIFICE 19

- SEP

- 02 12 2 P

4534 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

4535 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

4536 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

4537 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

4538 HS HUMANE SACRIFICE 3

- SEP

- 02 106 P

4539 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

4540 TERMINAL SACRIFICE 18

- SEP

- 02 121 P

4541 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

4542 TERMINAL SACRIFICE 23

- SEP

- 02 126 P

4543 NNMD TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

4544 NNMD TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

4545 TERMINAL SACRIFICE 20

- SEP

- 02 123 P

4546 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

4547 TERMI NAL SACRIFICE 18

- SEP

- 02 121 P

4548 TERMINAL SACRIFICE 17

- SEP

- 02 120 P

4549 TERMINAL SACRIFICE 21

- SEP

- 02 124 P

4550 TERMINAL SACRIFICE 19

- SEP

- 02 122 P

4551 TERMINAL SACRIFICE 5

- SEP

- 02 108 NP

-----------------------------------------------------------------------------------------------------------------------------------

NP

- NOT PREGNANT, P

- PREGNANT

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE

# Huntingdon Life Sciences 00

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211

- DIPE

- 1G Final Report

Individual Clinical Observations

Preface Appendix D

For summarization purposes, descriptive comments [i.e., location of scab(s) and

sore(s), etc.] are not presented in this appendix. These data are contained in the

study raw data if needed.

Deaths occurring noted between scheduled weekly examinations are presented

at the previous interval. The actual day of an animal’s death is presented in

Appendix C.

Corresponding exposure levels for each group were as follows:

Group I

- 0 mg/m 3



# Group II

- 2,000 mg/m 3



# Group III

- 10,000 mg/m 3



# Group IV

- 20,000 mg/m 311

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

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INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

1026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1029 WITHIN NORMAL LIMITS P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 3 3 3 3 2

ALOPECIA

- GENERAL 2 2

1030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1033 WITHIN NORMAL LIMITS P P P P P P P P P

SWOLLEN PAW(S) P P P

RIGHT FORE PAW

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2

CHROMODACRYORRHEA

- BILATERAL P P

CHROMODACRYORRHEA

- UNILATERAL P P

INCISORS BROKEN/MISSING P P P P P P P P

1034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P

LACRIMATION

- UNILATERAL 2

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHO LE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-------------------------------------------------------------------------------------------------------------------------- ---------

1036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P

INCISORS BROKEN/MISSING P P P

1039 WITHIN NORMAL LIMITS P P P P P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P

INCISORS MALOCCLUDED P P P P P P P

1040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1042 WITHIN NORMAL LIMITS P P P P P P P P P

SWOLLEN DIGIT P P P P P P P P P

FIRST DIGIT LEFT FORE PAW

1043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1045 WITHIN NORMAL LIMITS P P

ULCERATION

- CERVICAL P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 3 3

SCABS P P

CERVICAL AREA

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARK ED P

- PRESENT11

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

1046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

INCISORS BROKEN/MISSING P

1047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

1051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

2026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

INCISORS BROKEN/MISSING P

2027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

2036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2038 WITHIN NORMAL LI MITS P P P P P P P P P P P P P P P P P P

2039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P

INCISOR S BROKEN/MISSING P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SL IGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODU CTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

2040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2

2042 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

INCISORS BROKEN/MISSING P

2044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2046 WITHIN NORM AL LIMITS P P P P P P P P P P P P P P P P P P

2047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2048 W ITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2050 WITHIN NORMAL LIMITS P P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 2

2051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

3026 WITHIN NORMAL LIMITS P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 2 2 2 2

3027 WITHIN NORMAL L IMITS P P P P P P P P P P P P P P

INCISORS MALOCCLUDED P P P P

3028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3029 WITHIN NORMAL LIMITS P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 3 3 3 3 3 3 2 2

3030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P

3032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3035 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P

INCISORS BROKEN/MISSING P P

3036 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3037 WITHIN NORMAL LIMITS P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 2 3 3 3 3 3 3 3 3

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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AP PENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-------------------------------------------------------------------------------------------------------------------------------- ---

3038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3039 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2

3042 WITHIN NORMAL LIMITS P P P P P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P

INCISORS BROKEN/MISSING P P P P P P P

3043 WITHIN NORMAL LIMITS P P P P

HUMANE SACRIFICE P

CHROMODACRYORRHEA

- BILATERAL P

ORAL SORE P

3044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

FOUND DEAD P

3045 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3048 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDE NSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

3049 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

3051 WITHIN NORMAL LIMITS P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 3 3 3 3 3 3 3 3 3

ALOPECIA

- GENERAL 2 2 2 2 2 2 2 2 3 3 3

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION W HOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GRO UP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

------------------------------------------------------------------------------------------------------------------------ -----------

4026 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4027 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4028 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2

4029 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4030 WITHIN NORMAL LIMITS P P P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2

INCISORS BROKEN/MISSING P

INCISORS MALOCCLUDED P

4031 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4032 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P

SCABS P P P P

CERVICAL

4033 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4034 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4035 WITHIN NORMAL LIMITS P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 3 3 3 3 3 3 3 3

CHROMODACRYORRHEA

- BILATERAL P P P P P P P

INCISORS MALOCCLUDED P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

4036 WITH IN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4037 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4 038 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4039 WITHIN NORMAL LIMITS P P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P P P P P P P P P

LACRIMATION

- UNILATERAL 2

INCISORS BROKEN/MISSING P P P P P

INCISORS MALOCCLUDED P P P P P P P P P

4040 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4041 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4042 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4043 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4044 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4045 WITHIN NORMAL LIMITS P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P P

INCISORS BROKEN/MISSING P P P P P P P P P

INCISORS MALOCCLUDED P P P P P P P P P P P

4046 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

MALES GROUP IV 20000 MG/M3

- ----------------------------------------------------------------------------------------------------------------------------- -----

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

4047 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4048 WITHIN NORMAL LIMITS P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 3 2 2 2 2 3 3 3

4049 WITHIN NORMAL LIMITS P P P P P P P P P P P

INCISORS BROKEN/MISSING P P P P P P P

4050 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

4051 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

1526 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1527 WITHIN NORMAL LIMITS P P P P P P P P P P P

INCISORS BROKEN/MISSING P

1528 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1529 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1530 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1531 WITHIN NORMAL LIMITS P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

1532 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1533 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1534 WITHIN NORMAL LIMITS P P P P P P P P P

SWOLLEN DIGIT P

RIGHT FORE PAW

SWOLLEN DIGIT P

THIRD DIGIT RIGHT FORE PAW

1535 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1536 WITHIN NORMAL LIMITS P P P P P P P P P P P

1537 WITHIN NORMAL LIMITS P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRE SENT11

- JAN

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

1538 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1539 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1540 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

1541 WITHIN NORMAL LIMITS P P P P P P P

CHROMODACRYORRHEA

- UNILATERAL P P P P P

LACRIMATION

- UNILATERAL 3 3 3 3

INCISORS BROKEN/MISSING P P P P P

1542 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1543 WITHIN NORMAL LIMITS P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 3

INCISORS MALOCCLUDED P

1544 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1545 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1546 WITHIN NORMAL LI MITS P P P P P P P P P P P

1547 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1548 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1549 WITHIN NORMAL LIMITS P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

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- 2010 16:33

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APPENDIX D P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATIO N REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

1550 WITHIN NORMAL LIMITS P P P P P P P P P P P P

1551 WITHIN NORMAL LIMITS P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

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GASOLINE DIPE VAPOR COND ENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP II 2000 MG/M3

-------------------------------------------------------------------------------------------------------------- ---------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

2526 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2527 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2528 WITHIN NORMAL LIMITS P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

2529 WITHIN NORMAL LIMITS P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2

2530 WITHIN NORMAL LIMITS P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 3

INCISORS MALOCCLUDED P

2531 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2532 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2533 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2534 WITHIN NORMAL LIMITS P P P P P P P P P P P

SWOLLEN DIGIT P

FOURTH DIGIT RIGHT FORE PAW

2535 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2536 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2537 WITHIN NORMAL LIMITS P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

2538 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2539 WITHIN NORMAL LIMITS P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 3 3

2540 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2541 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2542 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2543 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P P

2544 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

2545 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2546 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2547 WITHIN NORMAL LIMITS P P P P P P P P P P P P

2548 WITHIN NORMAL LIMITS P P P P P P P P P P P

2549 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

2550 WITHIN NORMAL LIMITS P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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GASOLINE DIPE VAPOR CONDE NSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

2551 WITHIN NORMAL LIMITS P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP III 10000 MG /M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBS ERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

3 526 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

3527 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3528 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3529 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3530 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

INCISORS BROKEN/MISSING P

3531 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3532 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3533 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3534 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3535 WITHIN NORMAL LIMITS P P P P P P P P P P P

3536 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3537 WITHIN NORMAL LIMITS P P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2

3538 WITHIN NORMAL LIMITS P P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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GASOLINE DIPE VAPOR CONDENSATE: A O NE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

3539 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3540 WITHIN NORMAL LIMITS P P P P P P P P P P P

3541 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3542 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3543 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3544 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3545 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3546 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3547 WITHIN NORMAL LIMITS P P P P P P P P P P P

3548 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3549 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3550 WITHIN NORMAL LIMITS P P P P P P P P P P P P

3551 WITHIN NORMAL LIMITS P P P P P P P P P P

ALOPECIA

- EXTREMITIES/SN OUT 2

ALOPECIA

- GENERAL 2 2

-----------------------------------------------------------------------------------------------------------------------------------

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY OF

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

4526 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4527 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4528 WITHIN NORMAL LIMITS P P P P P P P P P P P

ALOPECIA

- GENERAL 2

4529 WITHIN NORMAL LIMITS P P P P P P P P P P

INCISORS BROKEN/MISSING P

INCISORS MALOCCLUDED P P

4530 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

4531 WITHIN NORMAL LIMITS P P P P P P P P P P P

4532 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4533 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4534 WITHIN NORMA L LIMITS P P P P P P P P P P P P

4535 WITHIN NORMAL LIMITS P P P P P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 3 3

4536 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4537 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4538 WITHIN NORMAL LIMITS P P P P P P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OB SERVATIONS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

1 1

DAY O F

- 1 2 2 3 4 4 5 6 7 7 8 9 9 0 1

ANIMAL# OBSERVATIONS STUDY 7 0 7 4 1 8 5 2 9 6 3 0 7 4 1 8 5 2

-----------------------------------------------------------------------------------------------------------------------------------

4539 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4540 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4541 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4542 WITHIN NORMAL LI MITS P P P P P P P P P P P P

4543 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINA L SACRIFICE P

4544 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

4545 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4546 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4547 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4548 WITHIN NORMAL LIMITS P P P P P P P P P P P

4549 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4550 WITHIN NORMAL LIMITS P P P P P P P P P P P P

4551 WITHIN NORMAL LIMITS P P P P P P P P P P P P P P P P P

TERMINAL SACRIFICE P

-----------------------------------------------------------------------------------------------------------------------------------

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- MODERATE 3

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GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-------------------------------------------------------------------------------------------------------------------- ---------------

1526 WITHIN NORMAL LIMITS P P P P

1527 WITHIN NORMAL LIMITS P P P P

1528 WITHIN NORMAL LIMITS P P P P

1529 WITHIN NORMAL LIMITS P P P P

1530 WITHIN NORMAL LIMITS P P P P

1531 ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

ALOPECIA

- GENERAL 2 2 2

1532 WITHIN NORMAL LIMITS P P P P

1533 WITHIN NORMAL LIMITS P P P P

1534 TERMINAL SACRIFICE P

SWOLLEN DIGIT P P

MISSING DIGIT P P

1535 ALOPECIA

- EXTREMITIES/SNOUT 2

WITHIN NORMAL LIMITS P P P

1536 WITHIN NORMAL LIMITS P P P P

1537 WITHIN NORMAL LIMITS P P P P

1538 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

1539 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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- MODERATE 3

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GAS OLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

1541 CHROMODACRYORRHEA

- UNILATERAL P P P P

INCISORS BROKEN/MISSING P P P P

1542 WITHIN NORMAL LIMITS P P P P

1543 ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 2

1544 WITHIN NORMAL LIMITS P P P P

1545 WITHIN NORMAL LIMITS P P P P

1546 WITHIN NORMAL LIMITS P P P P

1547 WITHIN NORMAL LIMITS P P P P

1548 TERMINAL SACRIFICE P

WITHIN NORMAL LIMITS P P P P

1549 ALOPECIA

- EXTREMIT IES/SNOUT 2 2 2 2

1550 WITHIN NORMAL LIMITS P P P P

1551 WITHIN NO RMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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- SLIGHT 2

- MODERATE 3

- MARKED P

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- 2010 16:46

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APPENDIX E P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

2526 WITHIN NORMAL LIMITS P P P P

2527 WITHIN NORMAL LIMITS P P P P

2528 ALOPECIA

- EXTREMITIES/SNOUT 2 2 2

WITHIN NORMAL LIMITS P

2529 ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

2530 ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 3

2531 WITHIN NORMAL LIMITS P P P P

2532 WITHIN NORMAL LIMITS P P P P

2533 WITHIN NORMAL LIMITS P P P P

2534 WITHIN NORMAL LIMITS P P P P

2535 WITHIN NORMAL LIMITS P P P P

2536 HUMANE SACR IFICE P

CHROMODACRYORRHEA

- BILATERAL P

INC ISORS BROKEN/MISSING P

DECREASED FECAL VOLUME P

2537 WITHIN NORMAL LIMITS P P P P

2538 WITHIN NORMAL LIMITS P P P P

2539 ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 3

-----------------------------------------------------------------------------------------------------------------------------------

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- SLIGHT 2

- MODERATE 3

- MARKED P

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APPENDIX E P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

2540 WITHIN NORMAL LIMITS P P P P

2541 WITHIN NORMAL LIMITS P P P P

2542 WITHIN NORMAL LIMITS P P P P

2545 WITHIN NORMAL LIMITS P P P P

2546 WITHIN NORMAL LIMITS P P P P

2547 WITHIN NORMAL L IMITS P P P P

2548 ALOPECIA

- EXTREMITIES/SNOUT 2

ALOPEC IA - GENERAL 2 2 2 2

2550 WITHIN NORMAL LIMITS P P P P

2551 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

COD E: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

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APPENDIX E P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

3527 WITHIN NORMAL LIMITS P P P P

3528 WITHIN NORMAL LIMITS P P P P

3529 WITHIN NORMAL LIMITS P P P P

3531 WITHIN NORMAL LIMITS P P P P

3532 WITHIN NORMAL LIMITS P P P P

3533 ALOPECIA

- EXTREMITIES/SNOU T 2

WITHIN NORMAL LIMITS P P P

3534 WITHIN NORMAL LIMITS P P P P

3535 WITHIN NORMAL LIMITS P P P P

3536 WITHIN N ORMAL LIMITS P P P P

3537 WITHIN NORMAL LIMITS P P P P

3538 WITHIN NORMAL LIMITS P P P P

3539 WITHIN NORMAL LIMITS P P P P

3540 WITHIN NORMAL LIMITS P P P P

3541 WITHIN NORMAL LIMITS P P P P

3542 WITHIN NORMAL LIMITS P P P P

3543 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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- MODERATE 3

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APPENDIX E P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICA L OBSERVATIONS DURING GESTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

3544 WITHIN NORMAL LIMITS P P P P

3545 WITHIN NORMAL LIMITS P P P P

3546 WITHIN NORMAL LIMIT S P P P P

3547 WITHIN NORMAL LIMITS P P P P

3548 WITHIN NO RMAL LIMITS P P P P

3549 WITHIN NORMAL LIMITS P P P P

3550 WITHIN NORMAL LIMITS P P P P

3551 ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

ALOPECIA

- GENERAL 2 2 2 2

-----------------------------------------------------------------------------------------------------------------------------------

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- SLIGHT 2

- MODERATE 3

- MARKED P

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APPENDIX E P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHAL ATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CLINICAL OBSERVATIONS DURING GESTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

4526 WITHIN NORMAL LIMITS P P P P

4527 WITHIN NORMAL LIMITS P P P P

4528 WITHIN NORMAL LIMITS P P P P

4529 INCISORS MALOCCLUDED P P P P

4531 WITHIN NORMAL LIMITS P P P P

4532 WITHIN NORMAL LIMITS P P P P

4533 WITHIN NORMAL LIMITS P P P P

4534 WITHIN NORM AL LIMITS P P P P

4535 ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 3

AL OPECIA

- GENERAL 3 3 3

4536 WITHIN NORMAL LIMITS P P P P

453 7 WITHIN NORMAL LIMITS P P P P

4538 WITHIN NORMAL LIMITS P P P P

4539 WITHIN NORMAL LIMITS P P P P

4540 WITHIN NORMAL LIMITS P P P P

4541 WITHIN NORMAL LIMITS P P P P

4542 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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- SLIGHT 2

- MODERATE 3

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- 2010 16:46

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APPENDIX E P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL C LINICAL OBSERVATIONS DURING GESTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2

FEMALE# OBSERVATIONS GESTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5

-----------------------------------------------------------------------------------------------------------------------------------

4545 WITHIN NORMAL LIMITS P P P P

4546 WITHIN NORMAL LIMITS P P P P

4547 WITHIN NORMAL LIMITS P P P P

4548 WITHIN NORMAL LIMITS P P P P

4549 WIT HIN NORMAL LIMITS P P P P

4550 WITHIN NORMAL LIMITS P P P P

-----------------------------------------------------------------------------------------------------------------------------------

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- MODERATE 3

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APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

1526 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1527 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1528 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1529 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1530 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1531 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 3 3 3

ALOPECIA

- GENERAL 2 2 2 3 3 3

1532 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1533 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1535 WITHIN NORMAL LIMITS P P P

TERMINAL SACRIFICE P

ALOPECI A - EXTREMITIES/SNOUT 2 2

YELLOW ANO

- GENITAL STAINING 2

------------------------------------------------------------------------------------------------------------------------------- -------

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APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

1536 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1539 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1541 TERMINAL SACRIFI CE P

CHROMODACRYORRHEA

- UNILATERAL P P P P P P

INCISORS B ROKEN/MISSING P P P P P P

1542 WITHIN NORMAL LIMITS P P P P P

TER MINAL SACRIFICE P

EMACIATION P

1543 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 3

154 4 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1546 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

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- 420 5 PAGE 243

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

1549 ALOPECIA

- EXTREMITIES/SNOUT 2 2 3 3 3

TERMINAL SACRIFICE P

1 550 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

1551 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 244

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

2526 WITHIN NOR MAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2527 WIT HIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2528 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 2

252 9 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 2

2530 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 3 3 3

2531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2532 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2533 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2534 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2535 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 245

APPEND IX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- - ----

2537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2538 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2539 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 3 3 3

2540 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2541 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2542 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2543 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2546 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

2547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 246

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

2548 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 2

ALOPECIA

- GENERAL 2 2 2 2 2 2

2550 WITHIN NORMAL LIMITS P P P P P

TERMINAL SACRIFICE P

INCISORS BROKEN/MISSING P

2551 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 247

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

3527 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3528 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3529 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3532 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3533 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2 3

3534 WITHIN NORMAL LIMITS P P P P P

TERMINAL SACRIFICE P

3535 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3536 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SAC RIFICE P

3538 WITHIN NORMAL LIMITS P P P P P P

TERMI NAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGH T 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 248

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTI ON TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

3539 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3540 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3541 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3542 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRI FICE P

3543 WITHIN NORMAL LIMITS P P P P P P

TERMINA L SACRIFICE P

3544 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3545 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3546 WITHIN NORMAL LIMITS P

TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2 2

3547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3548 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 249

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

3549 WI THIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

3550 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

35 51 WITHIN NORMAL LIMITS P P

TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2 2 2

ALOPECIA

- GENERAL 2 2 2 2

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 250

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OB SERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

4526 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4527 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4528 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4529 TERMINAL SACRIFICE P

INCISORS MALOCCLUDED P P P P P P

4530 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4531 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4532 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4533 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4534 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4535 TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 3 3 3 3 3 3

ALOPECIA

- GENERAL 3 3 3 3 3 3

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 251

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

-------------------------------------------------------------------------------------------------------------------------------- - -----

4536 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4537 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4538 WITHIN NORMAL LIMITS P P P P

HUMANE SACRIFICE P

INCISORS MALOCCLUDED P

SWOLLEN SNOUT P

RED EXUDATE SNOUT P

4539 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4540 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4541 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4542 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4545 WITHIN NORMAL LIMITS P P P P

TERMINAL SACRIFICE P

ALOPECIA

- EXTREMITIES/SNOUT 2 2

4546 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT12

- JAN

- 2010 16:36

Huntingdon Life Sciences 00

- 4205 PAGE 252

APPENDIX F P0 GENERATION

GASOLINE DIPE VAPOR CONDENSAT E: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

--------------------------------------------------------------------------------------------------------------------------------- -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

--------------------------------------------------------------------------------------------------------------------------------- -----

4547 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4548 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4549 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

4550 WITHIN NORMAL LIMITS P P P P P P

TERMINAL SACRIFICE P

------------------------------------------------------------------------------------------------------------------------------- -------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT

# Huntingdon Life Sciences 00

- 4205 Page 253

211

- DIPE

- 1G Final Report

Individual Body Weights

(Premating/Mating/Postmating)

Preface Appendix G

No data is presented for the following animals at the stated intervals for the

following reasons:

Animal No. Day(s) Reason

3043 28 to 112 Sacrificed for Humane Reasons on Day 23

3044 112 Found Dead on Day 110

1540 112 to 119 Terminal Sacrifice on Day 1 08

1549 77 to 91 Total Litter Loss on Lactation Day 2, therefore weekly

body weights were resumed.

2543 98 to 119 Although no evidence of mating was observed, this

female delivered on Day 98. See Lactation Body

Weights.

2544 112 to 119 Terminal Sacrifice on Day 108

2549 112 to 119 Terminal Sacrifice on Day 108

3526 112 to 119 Terminal Sacrifice on Day 108

3530 112 to 119 Terminal Sacrifice on Day 108

3534 77 to 91 Total Litter Loss on Lactation Day 1, therefore weekly

body weights were resumed.

453 0 98 to 119 Although no evidence of mating was observed, this

female delivered on Day 94. See Lactation Body

Weights.

4543 112 to 119 Terminal Sacrifice on Day 108

4544 112 to 119 Terminal Sacrifice on Day 108

4551 112 to 119 Terminal Sacrifice on Day 10811

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 254

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

1026 142 209 267 320 364 397 427 457 483 503 514 525 544 552 557 574

1027 151 217 268 325 364 408 442 468 485 501 522 523 538 553 557 568

1028 140 204 254 298 343 380 406 430 456 471 486 492 503 513 519 526

1029 135 195 249 295 330 366 393 419 434 454 472 474 476 488 491 501

1030 151 228 284 337 379 40 9 434 462 482 493 500 515 519 538 539 558

1031 147 210 266 322 365 384 419 434 449 474 489 501 500 492 506 523

1032 166 242 297 349 4 04 412 448 478 496 514 523 546 543 562 567 583

1033 143 225 274 320 352 392 427 449 469 493 474 467 487 514 525 535

1034 133 189 238 283 321 348 372 395 413 430 448 456 473 480 482 498

1035 139 204 248 292 335 358 388 413 431 442 455 467 474 495 496 507

1036 143 220 281 343 386 407 431 464 483 496 510 516 527 536 548 573

1037 133 199 251 300 336 361 387 410 425 432 452 466 468 480 482 494

1038 154 224 275 326 360 396 417 444 459 486 493 503 520 534 544 555

1039 132 197 241 291 329 359 388 420 425 447 447 455 466 474 487 493

1040 135 213 277 337 387 427 465 499 521 552 560 584 593 607 615 632

1041 148 221 282 352 404 450 491 516 539 545 567 597 613 623 630 649

104 2 153 223 281 344 394 436 469 502 518 552 568 585 598 624 643 661

1043 130 192 240 282 309 318 335 352 373 381 392 405 409 411 414 428

1044 135 218 277 336 382 417 447 476 494 503 528 543 553 562 577 591

1045 164 226 275 319 361 382 408 430 446 466 475 495 501 518 524 532

1046 155 232 295 339 380 391 421 454 475 488 502 523 517 539 547 569

1047 141 197 243 290 339 365 398 412 438 452 462 478 475 486 491 497

1048 161 218 279 335 361 392 432 467 488 511 542 542 558 560 576 595

1049 148 216 268 324 360 395 423 451 466 489 516 527 540 556 568 580

1050 160 233 290 344 389 411 435 352 453 482 497 514 536 556 558 571

1051 137 199 252 289 327 364 384 398 415 427 443 449 487 469 472 485

MEAN 145 213 267 319 360 389 419 440 462 480 494 506 516 528 535 54 9

S.D. 10.4 14.1 17.6 22.6 26.6 29.4 33.4 40.8 37.8 40.3 42.2 4 5.5 46.4 49.7 52.0 54.4

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 255

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

2026 134 200 244 288 317 342 372 393 413 425 444 456 464 486 477 487

2027 141 208 253 29 6 335 363 391 415 429 445 454 467 478 485 493 498

2028 136 204 250 302 348 378 412 434 452 469 484 503 505 523 534 540

2029 147 217 2 72 327 378 412 448 473 492 507 527 534 538 556 557 560

2030 140 206 262 317 372 417 456 482 509 537 552 568 574 593 601 600

2031 140 205 259 307 345 374 401 424 434 448 466 469 474 491 493 499

2032 133 193 245 293 334 360 384 408 421 427 438 452 451 468 474 480

2033 130 211 276 336 387 416 450 479 500 521 542 564 570 593 605 612

2034 153 221 277 331 372 409 449 470 488 511 531 543 557 572 588 597

2035 144 209 260 304 336 360 371 397 405 426 441 447 455 475 479 489

2036 135 208 253 301 330 355 373 396 408 420 429 435 439 455 463 471

2037 154 219 268 322 362 389 423 456 479 492 509 522 526 543 547 553

2038 143 208 261 304 343 374 402 424 437 454 469 483 494 505 510 530

2039 152 214 263 314 354 383 418 434 457 469 491 506 522 531 544 553

2040 150 218 271 319 366 397 429 448 474 490 504 519 532 544 547 571

2041 163 230 288 338 383 412 444 475 492 514 525 541 550 562 577 592

2042 149 211 260 304 350 388 418 448 460 473 486 497 506 515 523 536

2043 161 223 278 330 379 414 453 484 514 529 555 560 574 595 597 614

2044 159 233 294 354 403 430 465 495 524 542 570 577 589 611 627 640

2045 166 237 301 364 405 443 485 519 541 561 578 594 600 607 622 642

2046 142 212 266 308 342 376 404 426 444 462 477 492 497 507 524 540

2047 133 200 253 307 351 387 416 444 468 485 509 515 533 551 554 568

2048 131 195 241 283 318 338 371 389 402 418 434 440 453 459 469 485

2049 155 226 288 346 392 440 483 523 555 569 599 614 625 641 647 666

2050 148 215 270 323 371 403 436 461 481 499 506 525 536 552 573 574

2051 137 192 231 272 316 345 379 398 422 438 451 471 488 499 508 516

MEAN 145 212 265 315 357 389 420 446 465 482 499 511 520 535 543 554

S.D. 10.5 11.9 16.9 21.9 25.8 29.6 34.7 38.6 43.1 45.0 48.3 49.5 50.4 51.5 53.6 55. 1

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 256

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

3026 1 33 203 256 308 352 396 424 454 469 494 507 516 521 532 547 552

3027 134 193 239 286 318 349 375 393 409 426 431 438 445 461 463 465

3028 154 217 275 329 373 413 451 476 501 524 539 556 560 579 582 593

3029 145 215 269 320 358 388 413 439 458 487 507 517 529 551 556 578

3030 141 216 280 342 393 436 474 498 517 548 552 576 577 594 596 609

3031 134 204 259 308 339 366 401 422 428 446 465 475 484 497 490 501

3032 142 198 248 291 327 354 376 396 420 438 448 462 470 476 487 482

3033 136 199 263 321 364 396 421 441 455 474 488 498 505 530 549 565

3034 149 216 271 329 366 391 430 456 467 493 504 519 531 543 559 579

3035 153 218 274 332 374 410 447 469 485 506 513 524 534 558 575 588

3036 130 193 243 295 330 358 382 398 413 433 435 435 452 464 466 473

3037 147 213 270 329 370 394 420 445 456 477 491 495 513 524 527 538

3038 140 200 259 310 349 374 401 425 449 470 473 491 495 501 515 523

3039 135 219 273 322 359 380 413 434 463 487 486 502 511 519 531 547

3040 137 216 277 337 384 420 458 486 509 526 544 554 571 591 603 622

3041 148 233 298 355 400 426 464 484 512 530 544 556 562 575 588 603

3042 157 228 288 343 380 404 429 440 462 476 490 495 499 509 510 523

3043 150 225 292 362 290

3044 154 212 268 302 335 359 383 399 423 448 463 476 483 502 504 513

3045 141 199 252 291 322 338 356 372 396 411 420 426 435 445 455 459

3046 156 224 276 317 345 370 392 420 425 437 453 460 469 479 485 499

3047 139 200 250 305 342 367 406 434 449 464 473 490 502 520 526 531

3048 132 191 243 289 336 363 388 411 431 457 466 476 485 505 514 526

3049 168 232 281 318 351 38 0 407 423 432 456 472 485 496 510 513 519

3050 162 232 296 359 405 439 469 502 526 558 571 592 605 611 616 630

3051 162 232 293 345 3 85 420 466 491 519 539 552 568 582 595 604 609

MEAN 145 213 269 321 356 388 418 440 459 480 491 503 513 527 534 54 5

S.D. 10.5 13.4 16.9 22.1 27.5 28.1 33.2 35.9 38.0 40.4 41.4 44.8 44.7 45.8 47.5 50.8

N 26 26 26 26 26 25 25 25 25 25 25 25 25 25 25 2511

- JAN

- 2010 16:53

Huntingdon Life Scien ces 00

- 4205 PAGE 257

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

4026 154 231 292 353 398 427 465 491 516 542 553 562 580 604 609 618

4027 142 205 263 306 344 368 396 415 437 449 457 465 468 472 482 498

4028 150 211 274 329 372 394 427 458 487 507 509 529 538 557 570 578

4029 170 236 303 364 414 451 495 518 544 569 581 592 606 622 632 642

4030 134 205 259 312 357 392 430 458 474 493 516 525 535 550 550 562

4031 141 206 256 299 330 347 366 380 400 418 429 441 444 457 466 480

4032 162 245 309 375 423 463 503 541 570 611 631 654 672 701 712 731

4033 130 207 255 312 358 389 414 439 452 454 472 480 489 506 506 511

4034 139 205 272 331 374 388 416 431 446 462 475 490 494 508 512 526

4035 156 226 275 321 354 381 407 433 455 452 413 448 462 481 509 502

4036 148 209 252 311 355 393 424 451 472 492 505 517 532 554 562 566

4037 137 205 257 307 346 368 396 417 431 454 459 472 480 493 513 524

4038 135 198 242 288 331 360 384 400 421 445 453 460 469 480 485 495

4039 152 217 274 304 349 373 393 412 436 453 467 475 488 502 517 528

4040 133 198 250 293 333 360 392 411 434 459 462 474 486 501 514 525

4041 142 213 257 306 334 354 380 410 435 441 458 470 478 486 501 504

4042 146 209 258 309 348 380 407 432 456 471 498 512 523 541 554 579

4043 161 224 276 324 358 394 412 434 447 465 463 475 481 496 502 501

4044 160 222 270 312 345 373 406 428 448 467 475 493 497 515 517 519

4045 140 210 268 331 378 403 424 483 480 500 514 520 535 552 558 562

4046 143 210 259 31 2 351 380 409 439 450 469 485 498 508 528 536 544

4047 154 220 269 326 375 412 429 459 482 502 528 519 537 550 564 574

4048 149 224 2 78 341 393 429 457 486 514 543 564 578 583 596 608 624

4049 136 206 255 305 338 368 399 417 425 447 462 356 442 475 494 505

4050 133 186 227 271 303 336 350 360 386 399 406 411 416 430 438 446

4051 132 204 258 310 343 378 411 428 449 466 478 488 502 512 518 528

MEAN 145 213 266 317 358 387 415 440 459 478 489 496 509 526 536 545

S.D. 10.8 12.8 17.6 22.7 27.1 30.1 34.8 40.1 41.5 46.6 51.3 58.8 55.5 58.1 57.7 59. 7

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 258

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GR OUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

1026 574 592

1027 578 590

1028 536 553

1029 507 520

1030 566 572

1031 530 547

1032 595 619

1033 552 555

1034 495 509

1035 521 530

1036 566 588

1037 493 516

1038 563 579

1039 495 500

1040 645 654

1041 660 679

1042 671 682

1043 433 441

1044 603 619

1045 541 553

1046 579 586

1047 515 530

1048 600 614

1049 587 596

1050 576 595

1051 490 501

MEAN 557 570

S.D. 55.9 57.0

N 26 2611

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 259

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

2026 498 500

2027 510 511

2028 554 564

2029 571 586

2030 609 625

2031 507 517

2032 482 490

2033 627 637

2034 604 610

2035 498 502

203 6 481 489

2037 558 573

2038 530 544

2039 560 569

2040 570 579

2041 597 601

2042 537 544

2043 628 642

2044 650 659

2045 644 660

2046 548 559

2047 582 587

2048 485 489

2049 674 686

2050 592 603

2051 526 537

MEAN 562 572

S.D. 56.0 58.0

N 26 2611

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 260

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

3026 558 573

3027 475 483

3028 607 623

3029 592 605

3030 612 622

3031 511 519

3 032 505 509

3033 577 588

3034 578 591

3035 606 623

3036 487 501

3037 557 576

3038 550 565

3039 557 564

3040 633 649

3041 604 615

3042 538 546

3044 518

3045 465 472

3046 515 545

3047 540 543

3048 539 549

3049 530 542

3050 638 648

3051 625 642

MEAN 557 570

S.D. 49.6 51. 6

N 25 2411

- JAN

- 2010 16:53

Huntingdon Life Sciences 00

- 4205 PAGE 261

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTIO N TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112

-----------------------------------------------------------------------------------------------------------------------------------

4026 628 641

4027 502 509

4028 586 599

4029 642 657

4030 577 588

4031 490 503

4032 756 779

4033 522 528

4034 543 552

4035 508 526

4036 589 596

4037 533 544

4038 501 516

4039 538 550

4040 537 548

4041 508 524

4042 585 584

4043 517 519

4044 527 535

4045 566 571

4046 554 567

4047 593 601

4048 634 639

4049 514 527

4050 456 456

4051 540 543

MEAN 555 566

S.D. 61.8 63.8

N 26 2611

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 262

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHAL ATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

1526 96 145 169 189 207 223 228 241 244 261 262 259

1527 105 148 174 198 213 231 241 254 260 269 275 277

1528 11 0 145 159 187 205 217 215 233 244 245 245 258

1529 117 154 174 194 214 219 232 245 256 254 263 263

1530 111 157 186 216 235 255 275 280 296 307 306 313

1531 115 154 170 188 199 223 235 244 247 247 257 262

1532 117 165 188 207 242 253 260 281 295 289 288 298

1533 112 161 183 216 238 256 262 282 287 295 292 295

1534 109 160 177 214 233 256 264 272 278 286 289 292

1535 94 145 167 202 219 239 252 261 283 285 295 306

1536 99 156 187 218 235 256 268 279 276 289 296 306

1537 103 162 192 213 238 243 259 275 277 275 282 288

1538 120 177 205 238 262 279 298 302 314 322 328 324

1539 119 176 203 230 252 273 285 294 296 302 312 312

1540 122 161 180 195 216 230 237 242 254 261 256 263 264 268 272 271

1541 103 152 180 204 229 246 247 267 279 287 284 2 97

1542 104 152 175 203 228 241 242 256 265 272 275 277

1543 113 151 187 211 229 238 255 272 279 274 293 298

1544 106 156 182 216 233 253 267 278 287 293 302 304

1545 114 151 169 188 207 218 223 238 245 248 249 258

1546 100 142 168 190 211 225 219 238 248 249 260 264

1547 113 158 180 210 230 245 245 261 266 273 269 283

1548 102 143 163 184 203 218 218 227 238 243 248 258

1549 112 147 171 197 214 235 237 255 256 260 270 266 297

1550 109 152 174 199 220 229 236 247 254 262 265 271

1551 105 151 175 199 218 229 241 187 249 258 265 268

MEAN 109 155 178 204 224 240 248 258 268 273 278 283 264 268 272 28 4

S.D. 7.5 8.9 11.2 13.7 15.6 17.0 21.0 24.5 20.3 21.2 21.3 20.6 0.0 0.0 0.0 18.2

N 26 26 26 26 26 26 26 26 26 26 26 26 1 1 1 211

- JAN

- 2010 16:55

Huntingdon Life Sciences 00 -4 205 PAGE 263

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

2526 100 140 159 187 207 206 229 232 243 253 239 253

2527 108 150 175 198 220 232 250 259 265 273 280 277

2528 114 159 184 209 224 242 250 269 273 284 277 291

2529 117 160 183 202 228 238 253 257 262 272 273 271

2530 104 147 176 200 218 241 249 253 270 278 276 283

2531 119 158 187 211 232 238 257 269 278 279 288 292

2532 121 179 201 222 228 253 254 270 283 284 288 298

2533 119 167 189 223 252 289 284 301 304 304 319 325

2534 114 159 189 212 229 255 273 273 285 288 285 300

2535 111 158 180 202 220 234 246 259 262 266 271 272

2536 116 146 174 194 215 232 243 253 253 264 26 0 263

2537 110 169 186 219 244 252 274 281 292 292 299 302

2538 112 166 202 236 259 280 304 321 319 3 38 344 339

2539 106 156 182 210 232 250 260 261 272 279 282 285

2540 103 146 175 193 206 226 239 246 242 256 264 259

2541 113 154 177 141 210 235 254 264 260 273 278 275

2542 109 158 184 208 227 242 248 261 266 274 271 288

2543 112 149 172 194 213 215 228 241 248 246 260 267 279 307 334

2544 93 140 168 192 215 232 243 257 265 273 268 278 278 278 276 282

2545 97 141 171 196 221 229 255 274 276 278 298 295

2546 96 149 174 196 221 229 239 258 264 270 282 284

2547 107 146 165 188 203 216 220 235 241 245 245 254

2548 103 139 165 184 210 223 233 239 253 260 263 268

2549 121 169 185 216 229 249 260 272 275 281 288 289 289 298 300 303

2550 100 148 18 3 181 201 208 210 226 230 237 237 248

2551 105 150 158 216 230 244 263 277 275 295 297 291

MEAN 109 154 179 201 223 238 251 262 268 275 278 283 282 294 303 292

S.D. 7.9 10.3 11.0 18.2 14.0 18.9 19.8 20.5 19.7 20.4 23.1 21.0 6.6 14.9 29.0 14. 5

N 26 2 6 26 26 26 26 26 26 26 26 26 26 3 3 3 211

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 264

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BOD Y

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

3526 109 148 172 204 227 233 256 266 271 280 282 286 280 294 279 292

3527 105 138 169 198 211 238 257 267 269 286 292 281

3528 122 162 198 230 260 274 303 318 324 324 340 334

3529 92 139 162 186 204 219 227 242 245 251 259 260

3530 112 166 190 209 236 252 263 283 291 298 300 303 312 308 306 325

3531 104 146 169 198 219 234 249 261 269 273 274 284

3532 99 138 163 189 205 211 230 246 249 255 261 264

3533 95 141 157 190 207 223 242 252 25 3 268 274 265

3534 117 157 172 206 225 236 241 261 267 263 271 277 312

3535 105 138 163 176 205 223 231 2 37 247 253 253 264

3536 107 139 164 186 195 214 233 241 247 253 252 264

3537 119 154 164 191 206 216 221 234 238 245 245 258

3538 114 160 184 218 235 253 260 282 281 296 307 301

3539 107 155 181 208 233 244 264 279 278 300 297 296

3540 113 155 181 197 222 240 255 263 276 286 289 290

3541 120 160 186 209 231 249 262 276 282 292 294 308

3542 100 138 165 179 204 217 221 243 250 267 270 274

3543 102 154 173 194 212 224 237 244 245 257 260 259

3544 113 148 173 197 214 209 232 242 246 250 242 255

3545 116 167 201 224 242 245 251 267 272 287 277 294

3546 116 161 190 211 234 249 268 279 286 290 301 297

3547 10 9 141 172 188 211 214 228 237 241 244 249 248

3548 101 154 186 212 234 251 262 264 279 284 284 283

3549 111 148 177 197 214 226 232 251 246 253 262 260

3550 112 147 170 195 220 225 246 256 259 265 270 273

3551 103 152 178 196 224 230 248 253 256 273 282 275

MEAN 109 150 175 199 220 233 247 259 264 273 276 279 296 301 293 31 0

S.D. 7.8 9.3 11.5 13.2 14.8 16.2 18.4 19.1 19.9 20.3 22.5 20.1 22.6 10.0 19.4 16. 7

N 26 26 26 26 26 26 26 26 26 26 26 26 2 2 2 311

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 265

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL#

- 7 0 7 14 21 28 35 42 49 56 63 69 77 84 91 98

-----------------------------------------------------------------------------------------------------------------------------------

4526 109 145 165 192 205 224 230 248 257 259 260 268

4527 107 146 169 196 208 225 244 254 255 262 266 266

4528 117 165 182 212 229 249 255 279 287 294 286 304

4529 110 161 188 209 233 254 258 280 291 295 306 306

4530 111 153 175 202 217 239 25 3 265 262 285 286 286 303 325 368

4531 116 159 182 200 220 232 244 248 273 266 272 278

4532 118 151 172 192 209 2 22 237 244 246 248 256 260

4533 104 146 168 184 200 210 230 234 243 243 251 253

4534 105 150 171 187 200 211 229 235 237 251 254 252

4535 120 169 204 228 259 260 278 292 297 295 309 315

4536 121 176 192 225 248 264 272 286 297 302 300 310

4537 113 153 186 214 237 243 261 274 281 279 287 291

4538 94 144 174 201 219 236 247 249 264 276 278 284

4539 113 141 156 176 185 195 208 224 225 228 233 241

4540 105 158 188 225 232 275 289 295 304 317 336 341

4541 114 160 176 199 204 221 232 243 240 253 256 259

4542 112 146 158 185 195 206 215 230 235 239 236 247

4543 99 141 163 175 201 219 225 231 245 258 258 263 263 271 287 258

45 44 103 147 175 192 218 227 245 258 267 277 287 287 290 306 333 317

4545 102 148 169 191 208 224 237 249 251 264 277 277

4546 119 164 195 220 236 243 262 278 290 288 303 306

4547 103 155 181 213 234 255 269 286 299 311 312 332

4548 93 130 155 174 191 201 210 212 224 231 233 238

4549 113 154 171 184 203 216 228 231 249 259 256 262

4550 100 141 157 179 193 198 206 220 224 225 235 237

4551 107 150 168 194 205 219 230 247 247 248 256 261 263 261 270 279

MEAN 109 152 175 198 215 229 242 253 261 267 273 278 280 291 314 28 5

S.D. 7.6 10.1 12.5 16.2 18.8 21.1 21.8 23.8 25.3 25.7 27.3 28.7 2 0.2 29.6 44.5 29.9

N 26 26 26 26 26 26 26 26 26 26 26 26 4 4 4 311

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 266

APPENDIX G P0 GENERATION

GASOLI NE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

1540 282

1549 294 310 311

MEAN 288 310 311

S.D. 8.6 0.0 0.0

N 2 1 111

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 267

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE -B ODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

2544 294

2549 304

MEAN 299

S.D. 7. 1

N 211

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 268

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

I NHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

3526 295

3530 333

3534 306 307 312

MEAN 311 307 312

S.D. 19.8 0.0 0.0

N 3 1 111

- JAN

- 2010 16:55

Huntingdon Life Sciences 00

- 4205 PAGE 269

APPENDIX G P0 GENERATION

GASOLINE DIPE VAPOR COND ENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHTS (GRAMS)

FEMALES GROUP IV 20000 MG/M3

-------------------------------------------------------------------------------------------------------------- ---------------------

DAY OF STUDY

ANIMAL# 105 112 119

-----------------------------------------------------------------------------------------------------------------------------------

4543 271

4544 324

4551 275

MEAN 290

S.D. 29.5

N 3

# Huntingdon Life Sciences 00

- 4205 Page 270

211

- DIPE

- 1G Final Report

Individual Bod y Weight Gain

(Premating/Mating/Postmating)

Preface Appendix H

No data is presented for the following animals at the stated intervals for the

following reasons:

Animal No. Intervals Reason

3043 21

- 28

through

98

- 105 Sacrificed for Humane Reasons on Day 23

3044 105

- 112

and

0- 112 Found Dead on Day 110

Note: Body weight gains for females are presented for the premating period only

(Days

– 7 to 0 through 63

- 69 and 0

- 69).11

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 271

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHO LE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

1026 67 58 54 44 33 30 31 25 21 10 11 19 8 5 17 0

1027 66 51 57 39 44 33 27 16 16 22 1 15 15 5 11 10

1028 64 50 44 45 38 26 24 26 16 15 6 11 10 6 8 9

1029 60 55 46 35 35 27 26 15 20 18 2 3 12 3 10 7

1030 77 57 53 42 30 25 28 20 12 7 15 4 19 1 19 9

1031 63 57 56 43 20 35 15 15 24 15 13

- 1

- 8 14 17 7

1032 76 55 53 54 9 35 31 18 18 8 23

- 3 19 5 16 12

1033 82 48 46 32 40 35 22 21 24

- 19

- 8 20 28 11 9 18

1034 56 49 45 38 27 24 23 18 18 18 9 16 7 2 16

- 3

1035 66 44 44 43 24 30 25 17 11 14 11 8 21 1 11 14

1036 77 61 61 44 21 24 33 19 13 13 7 10 9 12 25

- 8

1037 67 52 48 36 25 26 24 15 7 19 14 2 13 2 12

- 1

1038 70 51 52 34 36 21 27 15 27 8 10 17 14 10 11 8

1039 65 44 50 37 30 29 32 5 23

- 1 8 11 9 13 6 2

1040 77 64 60 50 40 38 34 21 32 8 25 8 14 8 17 13

1041 73 60 7 0 52 46 42 24 23 6 22 30 16 10 7 19 11

1042 70 58 63 50 42 33 32 17 34 16 17 13 27 18 18 11

1043 62 47 42 27 9 17 17 20 9 11 13 4 2 4 14 5

1044 83 59 59 47 35 30 28 19 9 25 15 10 9 15 14 12

1045 63 49 44 42 21 26 22 16 21 9 20 6 18 6 9 8

1046 78 62 45 41 11 30 34 21 13 14 21

- 6 22 9 22 10

1047 57 46 47 49 26 32 15 26 14 10 16

- 3 11 5 6 18

1048 57 61 56 26 32 40 35 21 23 31

- 1 17 2 16 19 6

1049 68 52 57 35 35 28 28 15 23 26 11 14 15 12 13 7

1050 73 56 54 45 22 24

- 83 a 101 29 15 17 22 20 2 13 4

1051 62 53 37 38 37 20 15 17 12 16 6 38

- 18 2 13 6

MEAN 68 54 52 41 30 29 22 22 18 13 12 10 12 7 14 8

S.D. 7.8 5.7 7.6 7.2 10.5 6.1 22.2 16.8 7.5 9.5 8.3 9.5 9.9 5.1 4.8 5. 9

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2 6 26

a THIS ANIMAL’S WATER VALVE WAS NOTED AS NOT WORKING PROPERLY ON DAY 42. THE VALVE WAS REPAIRED IMMEDIATELY.11

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 272

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GE NERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MA LES GROUP II 2000 MG/M3

----------------------------------------------------------------------------------------------------------------------------- - -----

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

2026 66 44 44 30 25 30 22 20 12 19 12 7 22

- 9 10 11

2027 67 45 42 40 28 28 24 14 16 9 13 11 7 8 6 12

2028 68 46 52 47 30 34 22 18 17 15 19 2 18 11 6 14

2029 69 55 55 51 34 36 25 19 15 20 8 4 18 1 3 11

2030 67 56 55 55 46 39 27 27 27 15 16 6 19 8

- 1 9

2031 65 54 48 38 29 27 2 3 10 14 18 4 5 17 2 5 9

2032 60 53 48 41 26 24 24 13 6 11 13

- 1 17 6 6 3

2033 81 65 60 51 29 34 29 21 21 21 22 6 23 13 7 15

2034 68 57 54 41 37 40 21 18 23 20 12 15 15 15 9 7

2035 65 52 44 32 24 11 26 8 21 15 6 8 19 5 10 8

2036 73 46 47 30 25 18 23 13 12 9 6 4 15 9 8 10

2037 65 49 54 40 27 34 33 23 13 18 13 3 18 4 6 5

2038 65 53 43 39 31 28 22 12 18 14 15 11 11 5 20 0

2039 61 49 51 41 28 35 16 23 12 22 15 17 9 13 9 7

2040 68 53 48 47 32 32 19 27 15 14 15 14 11 3 24 0

2041 67 58 50 45 29 31 31 17 22 11 16 9 12 15 15 5

2042 62 49 44 46 39 29 31 11 14 12 11 9 9 8 13 1

2043 62 55 52 49 34 39 30 30 16 26 5 14 21 3 17 14

2044 74 61 60 49 27 35 30 29 18 28 7 12 22 16 13 10

20 45 72 64 63 42 38 42 34 23 19 17 16 7 6 15 20 2

2046 69 55 41 34 34 29 22 18 18 15 15 5 11 16 16 9

2047 67 54 54 44 36 30 28 24 18 24 5 19 18 2 15 14

2048 64 46 43 35 20 33 17 13 16 16 6 13 6 10 16 0

2049 71 62 58 46 47 43 40 32 15 30 15 11 16 6 18 9

2050 67 54 53 48 33 33 24 20 18 7 19 11 16 21 1 18

2051 55 39 42 44 29 34 19 24 15 14 20 17 11 9 8 9

MEAN 67 53 50 42 31 32 26 19 16 17 12 9 15 8 11 8

S.D. 5.0 6.4 6.3 6.7 6.3 7.0 5.7 6.5 4.3 5.8 5.1 4.9 5.1 6.4 6.3 5. 0

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 273

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

3026 70 53 52 44 44 28 30 16 24 13 9 5 11 15 5 6

3027 60 46 47 32 32 25 18 16 17 5 8 7 16 2 2 10

3028 63 58 55 43 40 38 25 25 24 14 18 4 18 3 11 14

3029 70 54 51 38 30 25 27 19 29 20 11 12 23 5 22 14

3030 75 64 62 51 42 38 24 18 32 4 24 1 17 2 13 3

3031 70 56 49 30 27 35 22 6 18 18 11 9 13

- 7 11 9

3032 56 50 43 37 27 22 20 24 18 11 13 8 6 11

- 5 23

3033 64 64 58 43 32 25 20 15 19 14 10 8 25 19 16 12

3034 6 7 55 58 37 26 38 27 11 26 11 15 12 13 16 20

- 2

3035 65 56 58 43 36 38 22 16 21 7 10 11 24 17 12 18

3036 62 50 52 35 28 24 16 15 20 2 0 17 12 2 8 14

3037 66 57 59 41 24 26 25 12 21 14 4 18 11 4 11 19

3038 60 59 51 39 25 27 24 23 21 4 18 4 6 14 9 27

3039 84 54 49 38 21 33 22 28 24 0 16 9 8 12 16 10

3040 79 61 60 47 36 38 28 23 17 18 10 17 20 12 19 11

3041 85 65 57 45 26 38 21 28 18 14 12 6 13 13 15 2

3042 72 60 55 37 24 25 11 22 14 14 6 3 10 1 14 14

3043 75 67 71

- 73

3044 58 56 35 33 24 24 16 24 25 15 13 7 19 2 9 5

3045 58 54 39 31 15 19 16 24 15 9 6 10 10 10 4 6

3046 68 51 41 29 25 22 28 5 12 15 8 9 10 5 14 17

3047 61 51 55 37 25 39 28 16 15 9 17 11 18 6 5 9

3048 59 52 46 47 27 25 23 20 26 9 9 10 20 9 12 13

3049 63 49 37 33 29 26 16 9 23 16 13 11 13 4 6 11

3050 70 64 63 46 34 30 33 24 32 12 22 12 6 5 14 8

3051 70 61 52 40 35 46 24 28 21 13 16 14 13 10 5 15

MEAN 67 56 52 35 29 30 23 19 21 11 12 9 14 8 11 1 2

S.D. 7.7 5.6 8.5 22.7 6.8 7.3 5.2 6.8 5.3 5.3 5.4 4.3 5.5 6.2 6.1 6.5

N 26 26 26 26 25 25 25 25 25 25 25 25 25 25 25 2511

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 274

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 69

- 77 77

- 84 84

- 91 91

- 98 98

- 105

-----------------------------------------------------------------------------------------------------------------------------------

4026 77 62 61 45 29 38 26 24 27 11 8 19 24 5 10 9

4027 64 57 44 38 24 28 19 22 12 9 8 3 4 9 16 4

4028 61 63 56 42 22 33 31 29 20 2 20 9 19 13 7 8

4029 67 66 61 51 37 43 23 26 24 12 12 13 17 10 10 1

4030 71 55 53 45 35 37 29 16 19 22 9 10 15 0 12 16

4031 65 50 42 32 17 19 14 20 18 11 12 3 12 9 14 10

4032 83 64 66 48 40 41 37 29 41 20 22 18 30 11 19 25

4033 77 48 58 46 31 25 25 13 3 17 9 9 17 0 5 11

4034 65 67 59 42 15 28 15 15 17 13 14 5 14 3 1 5 16

4035 70 49 46 33 27 27 26 21

- 3

- 38 35 14 19 28

- 7 6

4036 62 43 58 44 38 31 26 21 20 13 12 15 22 8 4 23

4037 68 52 50 39 22 29 21 15 23 5 12 9 13 20 11 9

4038 63 44 46 43 29 25 16 21 23 8 7 9 11 5 10 6

4039 65 57 30 45 25 20 19 24 18 14 8 13 15 14 12 10

4040 65 52 43 40 27 33 18 23 26 3 12 13 15 13 11 12

4041 71 44 49 28 21 26 29 25 6 17 12 8 8 15 3 4

4042 63 49 51 39 32 27 25 24 15 27 15 10 19 12 25 6

4043 63 53 47 35 36 18 22 14 17

- 2 12 6 15 6 0 15

4044 62 48 42 34 28 32 22 20 19 8 19 4 18 2 2 8

4045 70 58 63 47 26 21 59

- 3 21 14 6 14 17 6 4 4

4046 66 49 53 39 29 30 30 12 19 15 14 10 20 7 8 10

4047 66 49 57 49 37 18 30 23 19 27

- 9 18 13 14 10 19

4048 75 55 63 51 36 29 2 8 28 30 21 14 5 13 12 16 10

4049 70 48 50 34 29 32 17 8 22 15

- 106 86 33 20 10 9

4050 54 40 44 32 33 13 11 26 13 8 5 5 13 8 9 9

4051 72 53 53 33 35 33 18 21 17 13 9 14 10 7 10 11

MEAN 67 53 52 40 29 28 24 20 19 11 7 13 16 10 9 10

S.D. 6.2 7.2 8.4 6.6 6.6 7.4 9.3 7.3 8.5 12.3 24.3 15.6 6.1 6.5 6.4 5. 8

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 275

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

---------------------------------------------------------------------------------------------------------------------------- -------

1026 18 316 383

1027 13 306 374

1028 17 288 349

1029 13 279 326

1030 5 287 344

1031 17 292 337

1032 24 304 377

1033 3 242 330

1034 14 268 320

1035 9 262 326

1036 22 297 368

1037 23 267 316

1038 16 279 355

1039 5 258 303

1040 10 372 442

1041 20 376 458

1042 11 362 459

1043 8 213 249

1044 16 324 401

1045 12 269 327

1046 7 291 354

1047 15 281 332

1048 14 324 396

1049 8 311 380

1050 19 281 361

1051 1 0 250 302

MEAN 13 292 356

S.D. 5.7 38.2 48.5

N 26 26 2611

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 276

APPEND IX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

2026 2 256 300

2027 1 259 303

2028 10 299 361

2029 16 318 370

2030 16 362 419

2031 10 265 313

2032 8 259 297

2033 10 353 426

2034 7 322 390

2035 5 239 294

2036 8 227 281

2037 16 303 354

2038 14 276 336

2039 9 292 356

2040 9 300 361

2041 4 311 371

2042 8 286 334

2043 14 337 419

2044 10 344 426

2045 16 356 422

2046 11 28 0 347

2047 5 315 387

2048 4 245 294

2049 12 388 460

2050 11 310 387

2051 12 280 346

ME AN 9 299 360

S.D. 4.3 41.2 49.7

N 26 26 2611

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 277

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHO LE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

3026 15 313 370

3027 8 245 290

3028 16 339 406

3029 13 302 390

3030 10 360 406

3031 8 272 315

3032 4 264 310

3033 11 299 389

3034 13 303 376

3035 17 306 405

3036 14 242 309

3037 19 282 363

3038 15 291 365

3039 8 283 345

3040 16 338 433

3041 11 324 38 2

3042 8 267 317

3044 2 64

3045 8 227 274

3046 30 236 321

3047 3 291 343

3048 11 284 358

3049 12 254 310

3050 10 360 416

3051 17 336 410

MEAN 12 291 358

S.D. 5.6 37.6 44.5

N 24 25 2411

- JAN

- 2010 16:57

Huntingdon Life Sciences 00

- 4205 PAGE 278

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENER ATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 105

- 112 0

- 69 0

- 112

-----------------------------------------------------------------------------------------------------------------------------------

4026 14 331 410

4027 7 260 304

4028 13 318 388

4029 14 356 420

4030 11 320 384

4031 13 235 297

4032 24 408 534

4033 7 273 321

4034 10 285 348

4035 18 222 301

4036 7 308 387

4037 11 266 339

4038 15 262 318

4039 12 258 3 33

4040 12 276 351

4041 16 257 311

4042

- 1 303 375

4043 2 252 295

4044 9 272 314

4045 5 310 361

4046 13 289 358

4047 8 299 381

4048 5 354 416

4049 13 150 321

4050 1 225 270

4051 4 283 339

MEAN 10 284 353

S.D. 5.6 50.6 54.8

N 26 26 2611

- JAN

- 2010 17:00

Huntingdon Life Sciences 00

- 4205 PAGE 279

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

1526 49 24 20 18 16 5 13 3 17 1

- 3 114

1527 42 26 24 15 18 11 13 6 9 6 2 129

1528 35 14 28 18 12

- 2 19 11 1 0 13 112

1529 37 20 20 19 6 13 13 11

- 3 9 0 109

1530 47 29 30 19 20 20 5 15 12

- 2 7 156

1531 40 15 18 11 24 12 8 4

- 1 11 5 108

1532 48 24 19 35 11 7 21 14

- 6

- 1 10 134

1533 49 22 33 22 18 6 19 6 8

- 3 4 134

1534 51 17 37 1 9 23 9 8 6 8 3 3 132

1535 51 22 35 18 20 13 9 22 2 10 12 161

1536 57 31 31 17 21 13 11

- 4 13 7 10 150

1537 59 31 20 26 5 16 16 2

- 2 7 6 126

1538 57 28 33 25 17 19 4 12 8 5

- 4 147

1539 57 28 27 22 21 13 9 2 6 10 1 137

1540 39 19 14 22 14 6 6 11 8

- 5 7 102

1541 49 29 24 25 17 1 20 12 8

- 3 12 145

1542 48 23 28 25 14 1 13 9 7 3 2 125

1543 39 35 25 17 9 17 17 8

- 6 20 5 147

1544 49 27 33 17 21 14 11 9 6 10 2 149

1545 37 19 19 19 11 5 15 7 3 2 9 107

1546 42 26 22 21 14

- 5 19 10 1 11 5 123

1547 45 22 29 21 15 0 15 5 7

- 4 14 125

1548 42 20 21 19 15 0 9 11 5 6 10 115

1549 35 25 25 18 20 3 18 1 4 10

- 4 119

1550 43 22 25 21 10 7 11 8 8 3 6 119

1551 46 24 25 19 11 12

- 54 a 62 9 8 3 117

MEAN 46 24 26 20 15 8 10 10 5 5 5 129

S.D. 7.1 5.1 5.8 4.4 5.1 6.8 14.0 11.8 5.5 5.8 5.1 16.4

N 26 26 26 26 26 26 26 26 26 26 26 26

a THIS ANIMAL’S WATER VALVE WAS NOTED AS NOT WORKING PROPERLY ON DAY 42. THE VALVE WAS REPAIRED IMMEDIATELY.11

- JAN

- 2010 17:00

Huntingdon Life Sciences 00

- 4205 PAGE 280

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

2526 41 18 29 20

- 1 23 3 11 10

- 14 14 113

2527 42 25 24 21 12 18 9 6 7 7

- 2 128

2528 46 25 24 15 18 8 19 4 10

- 7 14 132

2 529 43 23 19 26 10 16 4 6 10 1

- 2 111

2530 43 30 24 18 23 8 4 17 8

- 3 7 136

2531 39 29 25 21 6 19 12 8 1 9 4 134

2532 58 22 21 7 25 1 16 14 1 5 9 119

2533 48 22 34 28 37

- 5 17 2 0 15 6 158

2534 45 30 23 17 26 18 0 12 3

- 2 15 141

2535 47 23 22 19 14 12 12 4 4 5 0 114

2536 30 28 20 21 18 11 10 0 11

- 4 3 117

2537 59 18 33 25 8 21 8 11

- 1 7 2 133

2538 53 37 34 22 21 24 17

- 2 19 7

- 5 17 4

2539 50 26 28 22 18 10 1 12 7 3 4 129

2540 43 29 18 13 21 12 8

- 5 15 8

- 5 113

2541 41 23

- 36 68 25 19 10

- 3 13 5

- 3 121

2542 49 26 24 19 15 6 13 5 8

- 3 17 130

2543 37 23 22 19 2 13 13 7

- 2 14 7 118

2544 48 28 24 23 17 11 14 8 9

- 5 10 138

2545 44 30 25 25 9 25 19 2 2 20

- 3 154

2546 52 26 22 25 8 9 19 6 6 12 2 135

2547 39 20 22 15 13 4 15 6 4

- 1 9 108

2548 36 26 19 26 13 10 6 14 7 3 6 130

2549 48 16 30 13 20 11 12 3 7 7 0 120

2550 47 36

- 3 20 7 2 17 4 6 0 11 100

2551 45 9 57 14 14 19 14

- 2 20 2

- 6 142

MEAN 45 25 22 22 15 13 11 6 7 4 4 129

S.D. 6.5 5.9 15.2 10.8 8.3 7.6 5.7 5.6 5.6 7.4 6.7 16.6

N 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 17:00

Huntingdon Life Sciences 00

- 4205 PAGE 281

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

3526 39 25 32 22 7 22 10 5 9 2 4 139

3527 34 31 29 13 27 19 10 2 17 6

- 11 143

3528 41 36 32 30 14 29 15 6

- 1 17

- 6 172

3529 46 24 23 19 15 8 15 2 7 7 2 122

3530 53 24 20 27 16 1 0 20 8 7 2 3 138

3531 43 23 29 22 14 15 13 8 5 1 11 138

3532 39 25 26 17 5 19 16 4 6 6 3 126

3533 47 16 33 17 16 19 10 1 15 6

- 8 124

3534 41 14 34 19 11 5 20 7

- 4 7 7 120

3535 32 25 13 30 18 8 6 11 6

- 1 12 127

3536 32 24 22 10 19 19 9 6 6

- 2 12 125

3537 35 10 27 15 10 5 13 3 8 0 13 104

3538 46 24 33 17 18 7 22

- 2 15 12

- 6 141

3539 48 27 26 26 11 20 15

- 1 22

- 3

- 1 141

3540 42 27 16 25 18 16 8 13 10 3 1 135

3541 40 25 24 22 18 14 13 6 10 2 14 147

354 2 38 27 14 26 13 4 22 7 17 3 4 136

3543 52 20 21 18 12 13 7 1 12 3

- 1 105

3544 34 26 24 17

- 5 23 10 4 4

- 8 13 107

3545 50 34 23 18 3 6 16 5 15

- 10 17 127

3546 45 29 21 23 15 19 12 6 5 10

- 4 136

3547 32 31 16 23 3 14 9 3 4 5

- 1 107

3548 53 32 26 21 17 12 2 15 5 0

- 1 129

3549 37 29 20 16 13 6 19

- 4 7 9

- 3 111

3550 35 23 25 25 5 21 10 4 6 4 3 126

3551 49 26 17 29 6 18 5 3 17 9

- 7 123

MEAN 42 25 24 21 12 14 13 5 9 3 3 129

S.D. 6.8 5.8 6.1 5.2 6.7 6.8 5.2 4.3 6.0 5.8 7.6 15.2

N 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 17:00

Huntingdon Life Sciences 00

- 4205 PAGE 282

APPENDIX H P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL BODY WEIGHT GAIN (GRAMS)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIM AL# -7- 0 0

- 7 7

- 14 14

- 21 21

- 28 28

- 35 35

- 42 42

- 49 49

- 56 56

- 63 63

- 69 0

- 69

-----------------------------------------------------------------------------------------------------------------------------------

4526 36 20 26 13 20 6 18 9 2 1 8 123

4527 39 23 26 12 17 19 10 1 7 4 0 120

4528 48 17 31 17 20 6 24 9 7

- 8 18 139

4529 51 27 21 24 21 4 22 12 4 11

- 1 145

4530 42 22 27 15 22 14 12

- 3 23 1 0 133

4531 43 23 18 20 12 13 4 24

- 7 7 6 120

4532 33 22 20 17 13 15 6 2 2 9 3 109

4533 42 22 17 16 9 21 4 9 0 8 2 10 7

4534 45 21 16 13 10 18 6 1 14 4

- 2 103

4535 50 34 25 30 1 18 14 4

- 2 13 7 146

4536 55 16 33 24 15 8 14 11 5

- 2 10 135

4537 40 33 29 23 6 18 13 7

- 2 8 3 138

4538 50 30 27 18 17 11 2 15 13 2 6 140

4539 28 15 20 9 10 13 16 1 3 5 7 100

4540 53 30 38 7 42 14 7 9 13 19 5 183

4541 46 16 23 5 17 11 11

- 3 14 3 3 99

4542 34 12 27 10 12 8 16 5 4

- 4 11 101

4543 42 22 12 26 18 6 6 14 12 0 6 122

4544 45 28 17 26 9 18 13 9 10 9 1 140

4545 47 21 22 17 16 13 13 2 13 13 0 129

4546 45 31 25 16 7 19 16 12

- 2 15 4 142

4547 51 27 32 2 1 21 14 17 13 13 1 20 178

4548 37 26 19 17 10 9 2 12 7 2 5 108

4549 41 17 13 19 14 11 3 18 9

- 2 5 108

4550 41 16 22 14 5 8 14 3 2 10 1 95

4551 43 18 26 11 14 11 17 0 1 8 5 111

MEAN 43 23 24 17 14 12 12 8 6 5 5 126

S.D. 6.5 6.0 6.2 6.2 7.9 4.8 6.1 6.7 6.8 6.3 5.3 22.7

N 26 26 26 26 26 26 26 26 26 26 26 26

# Huntingdon Life Sciences 00

- 4205 Page 283

211

- DIPE

- 1G Final Report

Individual Gestation Body Weights

Preface Appendix I

No data is presented for the following animal at the stated intervals for the

following reasons:

Animal No. Gestation

Days Reason

2536 4 to 20 Sacrificed for Humane Reasons on Gestation

Day 111

- JAN

- 2010 17:01

Huntingdon Life Sciences 00

- 4205 PAGE 284

APPENDIX I P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUA L GESTATION BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

1526 263 279 284 313 376

1527 266 293 301 324 384

1528 250 264 274 295 330

1529 264 285 295 325 378

1530 303 330 335 367 395

1531 263 285 291 325 377

1532 294 314 314 340 396

1533 295 314 320 353 424

1534x NP 283 307 315 343 327

1535 300 320 324 345 401

1536 297 325 339 373 432

1537 2 86 312 327 358 430

1538x NP 330 348 349 345 344

1539 309 332 345 372 435

1540xNNMD

1541 295 315 329 357 419

1542 284 298 305 329 390

1543 289 315 332 357 423

1544 310 334 338 367 423

1545 252 271 280 303 372

1546 253 289 292 324 390

1547 276 296 305 328 375

1548x NP 252 269 277 275 275

1549 TLL 276 293 302 322 387

1550 271 286 296 312 377

1551 256 277 282 299 313

MEAN 280 301 309 336 392

S.D. 19.3 20.7 21.6 24.4 31.2

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:01

Huntingdo n Life Sciences 00

- 4205 PAGE 285

APPE NDIX I P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

2526 257 283 296 329 405

2527 273 290 299 324 363

2528 291 315 325 342 413

2529 270 284 293 321 381

2530 283 304 308 338 400

2531 289 305 311 337 397

2532 291 312 320 346 404

2533 319 351 351 384 449

2534 294 311 320 350 409

2535 273 289 298 328 383

2536 HS 215

2537 283 310 319 347 372

2538 336 361 376 402 458

2539 285 302 310 335 399

2540 264 277 286 309 359

2541 282 303 313 341 409

2542 285 306 310 342 406

2543xPNMD

2544xNNMD

2545 296 326 337 367 426

2546 280 304 320 350 404

2547 251 267 271 298 353

2548 271 285 294 318 376

2549xNNMD

2550 245 270 282 305 326

2551 307 329 341 365 423

MEAN 280 304 313 340 396

S.D. 24.9 23.5 23.9 24.7 30.7

N 23 22 22 22 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:01

Huntingdon Life Sciences 00

- 4205 PAGE 286

APPENDIX I P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

3526xNNMD

3527 279 315 328 358 428

3528 335 367 381 415 471

3529 252 277 287 314 379

3530xNNMD

3531 282 300 309 327 369

3532 266 284 292 320 373

3533 268 294 303 337 393

3534 TLL 278 298 306 326 332

3535 259 282 288 315 383

3536 262 278 289 312 370

3537 258 287 304 334 358

3538 314 337 349 374 424

3539 298 331 344 370 423

3540 290 316 327 353 377

3541 312 327 338 373 436

3542 280 305 320 355 380

3543 263 279 287 311 381

3544 247 271 279 304 352

3545 293 323 338 358 414

3546 298 317 331 355 4 29

3547 241 256 263 289 340

3548 284 305 318 34 5 417

3549 265 280 291 316 389

3550 277 297 303 328 398

3551 283 307 313 342 419

MEAN 278 301 312 339 393

S.D. 22.4 25.0 26.8 28.2 33.6

N 24 24 24 24 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:01

Huntingdon Life Sciences 00

- 4205 PAGE 287

APPENDIX I P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICI TY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0 4 7 14 20

-----------------------------------------------------------------------------------------------------------------------------------

4526 262 289 293 319 365

4527 273 284 319 310 371

4528 282 317 320 347 417

4529 308 338 347 387 462

4530xPNMD

4531 270 292 307 319 392

4532 253 280 286 315 372

4533 252 272 279 307 345

4534 257 289 301 330 396

4535 307 326 342 373 433

4536 310 325 335 361 428

4537 290 321 337 370 427

4538 HS 276 307 317 354 415

4539 244 258 269 296 345

4540 326 360 372 391 455

4541 262 284 290 320 387

4542 239 257 266 291 320

4543xNNMD

4544xNNMD

4545 271 290 298 330 401

4546 310 333 347 386 442

4547 328 357 366 401 470

4548 2 34 257 261 293 355

4549 265 280 291 319 373

4550 236 252 260 291 359

4551x NP

MEA N 275 299 309 337 397

S.D. 28.8 32.1 33.7 35.5 41.6

N 22 22 22 22 22

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DAT E HS=HUMANE SACRIFICE

x=EXCLUDED FROM MEAN

# Huntingdon Life Sciences 00

- 4205 Page 288

211

- DIPE

- 1G Final Rep ort

Individual Gestation Body Weight Gain

Preface Appendix J

No data is presented for the following animal at the stated intervals for the

following reasons:

Animal No. Gestation

Days Reason

2536 0- 4 through

14

- 20 and

0

- 20 Sacrificed for Humane Reasons on Gestation

Day 111

- JAN

- 2010 17:03

Huntingdon Life S ciences 00

- 4205 PAGE 289

APPENDIX J P 0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

1526 16 5 29 63 113

1527 28 7 23 6 0 118

1528 13 10 21 35 80

1529 21 11 30 53 114

1530 27 6 32 28 92

1531 22 6 33 53 115

1532 20 1 26 56 103

1533 19 6 33 72 129

1534x NP 25 7 28

- 16 44

1535 20 4 21 56 101

1536 28 14 34 59 135

1537 27 14 32 72 145

1538x NP 18 0

- 4

- 1 14

1539 23 13 28 63 126

1540xNNMD

1541 20 14 28 62 123

1542 14 7 24 61 106

1543 26 17 25 66 134

1544 24 4 29 56 114

1545 19 9 23 69 120

1546 36 3 33 66 137

1547 20 9 24 47 98

1548x NP 16 8

- 2 0 23

1549 TLL 17 9 20 65 111

1550 15 11 16 65 106

1551 21 5 17 14 57

MEAN 22 8 26 56 113

S.D. 5.4 4.2 5.4 14.3 20.0

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:03

Huntingdon Life Sciences 00

- 4205 PAGE 290

APPENDIX J P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

2526 26 12 33 76 147

2527 17 9 25 39 90

2528 24 9 18 71 122

2529 15 9 28 60 112

2530 21 4 30 62 117

2531 15 6 26 61 108

2532 21 8 26 58 113

2533 32 0 34 65 130

2534 17 9 31 59 115

2535 16 9 30 55 110

2536 HS

2537 27 10 28 25 90

2538 25 15 26 56 122

2539 17 8 26 63 114

2540 13 9 23 50 96

2541 21 11 28 68 127

2542 22 3 32 64 121

2543xPNMD

2544xNNMD

2545 31 11 30 59 131

2546 24 16 29 55 124

2547 16 4 26 56 102

2548 14 9 24 58 106

2549xNNMD

2550 25 12 23 20 81

2551 21 12 24 59 116

MEAN 21 9 27 56 113

S.D. 5.3 3.8 3.8 13.2 15.5

N 22 22 22 22 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:03

Huntingdon Life Sciences 00

- 4205 PAGE 291

APPENDIX J P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

3526xNNMD

3527 36 13 29 71 149

3528 32 14 34 56 136

3529 24 10 27 65 126

3530xNNMD

3531 19 9 17 42 87

3532 19 7 28 53 107

3533 26 9 34 57 125

3534 TLL 19 9 20 6 54

3535 23 6 27 68 124

3536 17 10 24 57 108

3537 28 17 30 24 100

3538 23 11 26 49 110

3539 33 13 26 53 125

3540 26 11 26 24 87

3541 15 11 35 63 124

3542 25 15 36 25 100

3543 15 8 25 70 118

3544 25 7 26 47 105

3545 31 15 20 56 121

3546 19 14 24 74 131

3547 15 7 26 51 98

3548 21 14 27 71 133

3549 15 11 25 74 124

3550 20 6 25 70 121

3551 24 6 30 77 136

MEAN 23 11 27 54 115

S.D. 6.0 3.2 4.6 18.7 20.4

N 24 24 24 24 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:03

Huntingdon Life Sciences 00

- 4205 PAGE 292

APPENDIX J P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY S TUDY IN RATS

INDIVIDUAL GESTATION BODY WEIGHT GAIN (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

4526 27 3 27 46 103

4527 11 35

- 10 62 98

4528 35 3 27 70 135

4529 30 9 40 75 153

4530xPNMD

4531 22 15 13 73 122

4532 27 6 29 56 118

4533 20 7 28 38 93

4534 32 12 29 66 139

4535 19 16 31 60 126

4536 15 10 26 67 118

4537 31 17 33 57 137

4538 HS 31 10 36 61 139

4539 15 11 27 49 102

4540 34 13 19 64 130

4541 22 6 30 67 126

4542 18 9 25 29 81

4543xNNMD

4544xNNMD

4545 20 8 33 70 130

4546 24 14 38 56 132

4547 29 10 35 68 142

4548 23 4 32 62 120

4549 15 11 28 54 109

4550 15 8 31 68 123

4551x NP

MEAN 23 11 28 60 122

S.D. 7.0 6.7 10.2 11.4 17.8

N 22 22 22 22 22

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUM ANE SACRIFICE

x=EXCLUDED FROM MEAN

# Huntingdon Life Sciences 00

- 4205 Page 293

211

- DIPE

- 1G Final Report

Individual Maternal Lactation

Body Weights

Preface Appendix K

No data is presented for the following animals at the stated intervals for the

following reasons:

Animal No. Lactation

Days Reason

1549 4 to 28 Total Litter Loss on Lactation Day 2, therefore

weekly body weights were resumed.

2536 0 to 28 Sacrificed for Humane Reasons on Gestation

Day 1.

3534 4 to 28 Total Litter Loss on Lactation Day 1, therefore

weekly body weights were resumed.

4538 21 to 28 Sacrificed for Humane Reasons on Lactation

Day 14.11

- JAN

- 2010 17:04

Huntingdon Life Sciences 00

- 42 05 PAGE 294

APPENDIX K P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL M ATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 1 4 21 28

-----------------------------------------------------------------------------------------------------------------------------------

1526 288 311 306 322 315 268

1527 299 312 317 340 330 306

1528 263 276 273 290 287 269

1529 303 322 323 331 340 309

1530 343 348 335 351 302 327

1531 275 299 309 318 316 295

1532 316 329 334 358 345 316

1533 326 339 354 366 350 329

1534x NP

1535 312 259 237 316 315 311

1536 337 346 367 380 351 331

1537 338 349 340 369 374 342

1538x NP

1539 349 345 350 371 339 339

1540xNNMD

1541 320 344 360 363 349 335

1542 315 261 236 330 338 300

1543 323 340 344 350 342 311

1544 339 344 343 366 364 344

1545 277 297 301 312 306 284

1546 297 307 317 336 339 304

1547 317 332 321 329 316 291

1548x NP

1549 TLL 303

1550 285 303 301 324 310 268

1551 280 292 311 320 324 292

MEAN 309 317 318 340 331 308

S.D. 24.6 28.5 35.4 24.0 21.8 24.1

N 22 21 21 21 21 21

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:04

Huntingdon Life Sc iences 00

- 4205 PAGE 295

APPENDIX K P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

2526 312 329 333 335 275 303

2527 285 315 309 321 324 291

2528 288 306 326 351 334 330

2529 278 288 305 327 327 292

2530 299 324 326 345 341 291

2531 306 341 334 357 337 321

2532 299 319 320 340 331 309

2533 334 356 356 380 372 366

2534 303 330 329 342 337 326

2535 306 317 313 331 317 299

2536 HS

2537 315 331 332 343 346 348

2538 360 374 388 392 377 343

2539 307 320 320 336 327 315

2540 284 301 310 316 316 278

2541 313 320 330 346 330 310

2542 307 317 335 345 332 298

2543 PNMD 315 332 343 363 343 322

2544xNNMD

2545 328 358 366 377 366 331

2546 311 335 338 350 335 314

2547 265 278 304 323 309 265

2548 289 296 307 329 333 308

2549xNNMD

2550 261 296 307 329 332 293

2551 326 352 347 355 343 327

MEAN 304 323 329 345 334 312

S.D. 22.2 23.4 20.9 19.4 21.0 23.3

N 23 23 23 23 23 23

-----------------------------------------------------------------------------------------------------------------------------------

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:04

Hunti ngdon Life Sciences 00

- 4205 PAGE 296

APPENDIX K P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal No. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

3526xNNMD

3527 316 343 348 357 354 303

3528 369 390 391 411 405 355

3529 291 321 320 328 324 287

3530xNNMD

3531 285 304 313 330 326 310

3532 299 312 315 342 329 297

3533 292 323 321 352 340 305

3534 TLL 306

3535 281 276 298 318 315 275

3536 266 295 3 09 329 328 304

3537 304 319 320 346 330 288

3538 342 33 9 337 355 347 326

3539 350 350 352 362 374 343

3540 307 316 329 363 359 320

3541 332 349 356 360 351 339

3542 323 334 334 347 354 316

3543 286 294 304 330 333 291

35 44 271 297 308 326 322 287

3545 333 333 344 360 355 336

3546 327 347 360 370 366 335

3547 265 270 291 311 308 275

3548 318 340 340 362 376 319

3549 280 300 308 318 322 289

3550 274 305 312 327 329 309

3551 316 330 334 365 355 318

MEAN 306 321 328 346 343 310

S.D. 27.8 27.3 23.4 22.6 23.1 22.3

N 24 23 23 23 23 23

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:04

Huntingdon Life Sciences 00

- 4205 PAGE 297

APPENDIX K P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

---------------------------------------------------

Animal N o. 1 4 7 14 21 28

-----------------------------------------------------------------------------------------------------------------------------------

4526 273 302 298 308 312 2 88

4527 293 295 308 328 327 306

4528 311 320 323 335 35 0 308

4529 353 356 371 401 388 353

4530 PNMD 274 328 328 340 327 307

4531 323 331 346 360 356 317

4532 282 295 312 337 331 300

4533 282 307 309 327 333 303

4534 301 320 327 349 336 300

4535 318 342 345 360 361 352

4536 323 340 347 372 372 345

4537 330 341 338 360 348 312

4538 HS 319 320 345 362

4539 271 290 302 316 315 300

4540 355 374 383 390 392 359

4541 299 322 319 349 324 290

4542 277 286 297 321 303 268

4543xNNMD

4544xNNMD

4545 307 311 317 344 347 293

4546 328 354 364 401 393 352

4547 372 372 386 399 387 365

4548 258 278 297 302 313 271

4549 297 309 311 316 323 280

4550 252 283 287 318 316 282

4551x NP

MEAN 304 321 329 348 343 311

S.D. 31.5 27.5 28.2 30.1 28.3 29.9

N 23 23 23 23 22 22

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE

x=EXCLUDED FROM MEAN

# Huntingdon Life Sciences 00

- 4205 Page 298

211

- DIPE

- 1G Final Report

Individual Maternal Lactation

Body Weight Gain

Preface Appendix L

No data is presented for the following animals at the stated intervals for the

following reasons:

Animal No. Lactation

Days Reason

1549 1- 4 through

21

- 28 and

1

- 28 Total Litter Loss on Lactation Day 2, therefore

weekly body weights were resumed.

2536 1-4 through

21

- 28 and

1- 28 Sacrificed for Humane Reasons on Gestation

Day 1.

3534 1- 4 through

21

- 28 and

1

- 28 Total Litter Loss on Lactation Day 1, therefore

weekly body weights were resumed.

4538 14

- 21

through

21

- 28 and

1

- 28 Sacrificed for Humane Reasons on Lactation

Day 14.11

- JAN

- 2010 17:06

Huntingdon Life Sciences 00

- 4205 PAGE 299

APPENDIX L P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICIT Y STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7

- 14 14

- 21 2 1

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

1526 23

- 5 17

- 7

- 47

- 19

1527 13 5 23

- 10

- 24 7

1528 13

- 3 17

- 3

- 18 5

1529 19 2 8 9

- 31 6

1530 5

- 14 16

- 49 25

- 16

1531 24 10 10

- 2

- 21 21

1532 12 5 24

- 13

- 29

- 1

1533 13 15 12

- 16

- 21 3

1534x NP

1535

- 52

- 22 79

- 1

- 4

- 1

1536 9 21 13

- 29

- 19

- 6

1537 11

- 9 29 5

- 33 3

1538x NP

1539

- 4 5 22

- 32 0

- 10

1540xNNMD

1541 23 16 4

- 14

- 15 14

1542

- 53

- 25 94 8

- 38

- 14

1543 17 4 6

- 7

- 32

- 13

1544 5

- 1 23

- 2

- 20 5

1545 21 3 11

- 6

- 22 7

1546 10 10 19 4

- 35 7

1547 15

- 11 9

- 13

- 25

- 26

1548x NP

1549 TLL

1550 18

- 2 23

- 14

- 42

- 17

1551 13 18 9 4

- 31 13

MEA N 7 1 22

- 9

- 23 -1

S.D. 21.1 12.5 22.5 14.2 15.8 12.5

N 21 21 21 21 21 21

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=T OT AL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:06

Huntingdon Life Sciences 00

- 4205 PAGE 300

APPENDIX L P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATI ON

FEMALE# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

2526 17 4 2

- 60 28

- 9

2527 31

- 7 12 3

- 33 6

2528 18 20 24

- 16

- 4 42

2529 10 18 21 1

- 36 14

2530 25 2 18

- 4

- 50

- 8

2531 35

- 6 23

- 20

- 16 16

2532 21 0 21

- 10

- 22 11

2533 22 0 24

- 7

- 7 31

2534 27

- 1 13

- 5

- 11 23

2535 12

- 4 18

- 14

- 18

- 7

2536 H S

2537 16 0 11 3 3 33

2538 14 15 4

- 15

- 34

- 17

2539 13 0 16

- 9

- 12 8

2540 17 9 6 0

- 38

- 5

2541 7 10 16

- 16

- 20

- 3

2542 10 18 10

- 14

- 34

- 9

2543 PNMD 17 12 20

- 20

- 21 7

2544xNNMD

2545 30 8 12

- 11

- 36 2

2546 24 4 12

- 15

- 21 3

2547 13 26 19

- 14

- 44 0

2548 7 11 22 4

- 25 19

2549xNNMD

2550 35 11 22 3

- 39 32

2551 27

- 6 9

- 13

- 16 2

MEAN 19 6 15

- 11

- 22 8

S.D. 8.5 9.1 6.6 13.2 17.3 15.8

N 23 23 23 23 23 23

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:06

Huntingdon Life Sciences 00

- 4205 PAGE 301

APPENDIX L P0 GENERATION

GASOLINE DIPE VAPOR COND ENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP III 10000 MG/M3

-------------------------------------------------------------------------------------------------------------- ---------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

----------------------------------------------------------------------------------------------------------------------------- ------

3526xNNMD

3527 28 4 10

- 4

- 50

- 13

3528 21 1 20

- 6

- 50

- 14

3529 30 0 8

- 4

- 37

- 4

3530xNNMD

3531 19 9 17

- 4

- 16 25

3532 13 3 27

- 14

- 32

- 2

3533 30

- 2 31

- 12

- 35 13

3534 TLL

3535

- 5 22 20

- 4

- 40

- 6

3536 29 13 20 0

- 24 38

3537 15 1 26

- 16

- 42

- 16

3538

- 3

- 2 19

- 9

- 21

- 16

3539 0 3 10 12

- 31

- 7

3540 9 13 34

- 4

- 39 13

3541 17 7 4

- 9

- 12 7

3542 11 0 12 7

- 38

- 7

3543 8 10 26 3

- 42 5

3544 26 12 18

- 5

- 35 16

3545 0 10 16

- 5

- 19 3

3546 20 12 11

- 4

- 31 7

3547 5 21 20

- 3

- 32 11

3548 23 0 22 14

- 57 1

3549 20 8 10 5

- 34 8

3550 31 7 15 2

- 20 35

3551 14 4 30

- 9

- 38 1

MEAN 16 7 18

- 3

- 34 4

S.D. 11.2 6.7 8.0 7.5 11.3 14.8

N 23 23 23 23 23 23

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:06

Huntingdon Life Sciences 00

- 4205 PAGE 302

APPENDIX L P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION BODY WEIGHT GAIN (GRAMS)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

FEMALE# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

4526 29

- 3 9 4

- 24 15

4527 3 13 20

- 1

- 22 13

4528 9 3 13 15

- 42

- 4

4529 3 15 30

- 13

- 35 0

4530 PNMD 54 0 12

- 13

- 19 34

4531 7 15 15

- 4

- 38

- 6

4532 13 17 26

- 6

- 31 18

4533 25 2 18 5

- 30 20

4534 19 7 22

- 14

- 35

- 1

4535 23 3 15 1

- 10 33

4536 16 7 25 0

- 26 22

4537 11

- 3 22

- 12

- 36

- 18

4538 HS 1 25 18

4539 20 12 13 0

- 15 30

4540 19 9 7 2

- 33 4

4541 22

- 2 30

- 25

- 35

- 10

4542 8 11 24

- 18

- 35

- 10

4543xNNMD

4544xNNMD

4545 4 5 27 4

- 55

- 14

4546 26 10 37

- 8

- 42 24

4547 0 14 13

- 13

- 22

- 6

4548 20 20 5 10

- 42 13

4549 13 2 5 8

- 43

- 16

4550 31 4 31

- 2

- 34 30

4551x NP

MEAN 16 8 19

- 4

- 32 8

S.D. 12.4 7.5 8.9 9.9 10.5 17.1

N 23 23 23 22 22 22

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE

x=EXCLU DED FROM MEAN

# Huntingdon Life Sciences 00

- 4205 Page 303

211

- DIPE

- 1G Final Report

Individual Feed Consumption

(Premating/Mating/Postmating)

Preface Appendix M

No data is presented for the following animals at the stated intervals for the

following reasons:

Animal No. Day(s) Reason

3043 28 to 112 Sacrificed for Humane Reasons on Day 23

3044 112 Found Dead on Day 110

1540 112 Terminal Sacrifice on Day 108

154 9 91 to 98 Total Litter Loss on Lactation Day 2, therefore weekly

feed consumption was resumed.

2543 98 to 112 Although no evidence of mating was observed, this

female delivered on Day 98. See Lactation Feed

Consumption.

2544 112 Terminal Sacrifice on Day 108

2549 112 Terminal Sacrifice on Day 108

3526 112 Terminal Sacrifice on Day 108

3530 112 Terminal Sacrifice on Day 108

3534 91 to 98 Total Litter Loss on Lactation Day 1, therefore weekly

feed consumption was resumed.

4530 98 to 112 Although no e vidence of mating was observed, this

female delivered on Day 94. See Lactation Feed

Consumption.

4543 112 Terminal Sacrifice on Day 108

4544 112 Terminal Sacrifice on Day 108

4551 112 Terminal Sacrifice on Day 10811

- JAN

- 2010 17:07

Huntingdon L ife Sciences 00

- 4205 PAGE 304

APPENDI X M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

1026 123 108 99 96 84 75 74 70 58 56 55 49 53 46 49

1027 121 98 89 77 70 66 64 58 56 54 50 47 45 47 46

1028 122 98 88 78 72 67 66 61 57 53 55 48 47 46 48

1029 123 102 86 75 69 66 64 61 46 56 51 50 51 52 49

1030 122 99 88 78 71 65 61 59 56 52 49 48 48 47 47

1031 125 101 89 78 69 66 58 58 58 53 52 52 47 47 49

1032 120 100 84 76 58 64 62 58 58 5 4 55 47 49 48 48

1033 130 97 83 71 71 69 67 63 62 53 35 51 50 49 40

1034 120 96 85 74 69 64 63 59 59 58 53 48 47 45 46

1035 118 93 80 71 64 62 61 59 56 53 53 48 46 50 46

1036 125 103 94 82 67 64 63 61 59 55 52 49 52 46 47

1037 129 105 89 81 72 69 64 BF 58 56 55 51 50 49 52

1038 121 94 85 74 70 64 60 60 60 51 51 48 46 44 47

1039 129 100 87 77 71 67 65 60 59 53 56 52 46 49 46

1040 123 103 92 78 68 65 62 60 57 50 54 47 47 43 41

1041 125 102 92 78 72 66 62 59 56 53 57 46 48 47 47

1042 121 102 93 82 75 70 68 62 60 55 56 51 49 48 47

1043 129 108 89 76 69 71 67 70 64 62 61 53 54 53 55

1044 132 105 93 84 72 69 64 62 51 58 52 49 47 46 49

1045 117 95 89 81 64 65 65 61 59 57 56 49 47 46 46

1046 134 10 5 96 83 68 65 70 69 64 62 64 58 53 56 50

1047 121 100 93 87 73 70 65 65 62 59 57 49 49 54 52

1048 1 17 94 87 73 68 67 64 63 57 55 53 49 48 48 47

1049 124 100 89 76 72 70 64 64 61 59 54 52 50 47 46

1050 122 98 IW 81 68 67 27 83 67 59 56 50 52 47 47

1051 127 103 89 80 74 66 65 64 62 57 54 51 50 47 47

ME AN 124 100 89 79 70 67 63 63 59 55 54 50 49 48 47

S.D. 4.6 4.1 4.2 5.3 4.5 2.9 8.0 5.5 4.1 3.2 5.0 2.4 2.5 3.0 3.0

N 26 26 25 26 26 26 26 25 26 26 26 26 26 26 26

BF=Broken Feeder IW=Invalid Weight: Feed left greater than feed fed weight11

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 305

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

2026 130 100 86 74 69 67 64 61 58 57 55 47 48 52 50

2027 124 94 81 71 67 64 59 58 57 53 51 47 47 48 45

2028 130 100 87 77 69 65 62 56 56 54 55 46 46 46 45

2029 122 94 84 76 71 64 64 60 56 58 52 44 43 47 48

2030 130 105 90 83 76 70 66 63 4 9 54 54 49 45 46 45

2031 133 106 92 83 76 71 67 60 60 59 55 51 49 53 51

2032 122 100 87 77 70 64 62 56 54 54 53 45 44 45 47

2033 138 105 90 79 70 64 65 62 59 57 55 50 47 49 47

2034 121 97 85 73 73 67 60 57 47 52 50 44 43 43 43

2035 122 98 83 72 68 63 64 60 49 54 51 48 48 49 48

2036 131 115 97 81 78 68 68 67 63 60 58 55 58 56 54

2037 121 93 86 75 69 68 65 62 57 55 51 45 45 43 45

2038 128 102 82 74 69 63 60 57 53 53 52 46 48 45 44

2039 122 97 88 80 77 65 63 61 50 55 56 49 48 45 46

2040 124 97 83 74 71 67 63 62 58 52 53 48 48 46 45

2041 117 99 84 73 69 65 61 57 48 52 51 47 45 44 43

2042 122 95 85 77 70 65 63 57 53 51 50 46 45 43 42

2043 121 94 86 79 72 68 66 63 41 56 49 44 45 45 45

2044 12 9 99 85 77 67 66 65 63 57 54 54 47 45 44 44

2045 123 96 84 72 68 63 62 61 49 52 51 47 46 45 46

2046 132 100 85 76 71 66 61 60 57 56 57 51 51 49 47

2047 132 103 89 76 69 63 62 61 57 56 52 46 44 46 45

2048 128 99 87 77 67 65 66 60 60 56 52 50 48 48 43

2049 124 99 87 78 74 68 65 62 56 56 52 44 46 46 44

2050 129 105 87 78 72 68 60 60 58 55 54 48 47 48 46

2051 125 98 83 77 71 66 64 62 59 56 55 48 48 47 46

MEAN 126 99 86 77 71 66 63 60 55 55 53 47 47 47 46

S.D. 5.0 4.8 3.4 3.1 3.2 2.1 2.4 2.6 5.1 2.4 2.2 2.7 2.9 3.2 2.6

N 26 26 26 26 26 26 26 26 26 26 26 26 26 26 2611

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 306

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GEN ERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MAL ES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

3026 132 100 95 96 80 71 68 64 52 55 54 52 45 47 49

3027 128 101 87 75 75 68 63 60 57 54 51 52 50 50 50

3028 121 98 86 76 69 67 61 61 58 53 54 48 47 45 46

3029 128 99 89 78 70 67 65 63 60 57 54 48 49 50 49

3030 129 100 83 75 69 64 57 57 56 50 50 45 44 43 43

3031 138 108 87 75 71 69 6 5 SF 59 56 53 49 51 48 48

3032 120 96 85 75 66 66 63 61 54 53 51 49 46 51 49

3033 126 105 90 76 69 67 65 61 57 55 52 54 51 51 49

3034 125 99 89 76 70 70 64 60 58 53 58 49 48 47 48

3035 122 97 85 74 68 66 62 57 54 52 50 49 46 49 50

3036 131 98 86 74 68 67 65 59 58 52 52 48 47 51 50

3037 129 106 91 77 70 68 67 64 59 58 55 50 49 55 53

3038 123 98 85 75 65 67 65 65 59 53 55 50 50 53 50

3039 139 95 84 75 65 68 64 66 52 52 55 49 50 48 45

3040 139 103 90 79 72 67 63 63 50 45 54 50 48 47 47

3041 BF 102 90 77 69 67 66 64 59 54 54 49 49 47 46

3042 126 98 87 73 66 64 62 61 58 54 54 49 50 51 48

3043 133 104 91 39

3044 121 93 80 71 65 65 61 61 58 54 52 47 46 39

30 45 120 97 85 75 63 66 60 62 56 59 53 52 50 50 47

3046 128 98 83 73 66 67 63 61 57 50 52 48 48 50 48

3047 129 100 87 78 73 68 67 62 58 56 57 52 52 51 47

3048 129 100 87 78 69 66 65 64 60 59 55 48 50 49 46

3049 130 100 86 75 71 70 67 63 60 55 57 50 49 50 46

3050 134 101 90 79 73 70 66 62 55 48 58 49 49 50 46

3051 129 98 87 76 72 71 67 64 56 53 55 49 45 48 47

MEAN 128 100 87 75 69 67 64 62 57 54 54 49 48 49 48

S.D. 5.6 3.4 3.2 8.6 3.7 1.9 2.6 2.3 2.7 3.2 2.2 1.9 2.1 3.2 2.2

N 25 26 26 26 25 25 25 24 25 25 25 25 25 25 24

SF=Spilled Feeder BF=Broken Feeder11

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 307

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDEN SATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

MALES GROUP IV 20000 MG/M3

---------------------------------------------------------------------------------------------------------------- -------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

4026 130 99 85 49 65 65 61 59 49 51 52 45 44 45 46

4027 123 95 82 73 68 68 60 59 56 55 51 51 48 49 48

4028 125 100 91 82 SF 73 70 69 57 54 55 50 48 47 45

4029 121 97 84 73 67 6 5 57 57 54 51 50 47 46 44 43

4030 128 96 83 71 67 63 60 57 53 53 50 45 44 46 46

4031 135 99 85 74 65 64 61 62 57 54 55 47 49 51 47

4032 131 93 84 74 67 64 61 59 57 43 52 46 46 47 44

4033 140 97 87 85 68 66 62 60 57 55 55 49 48 50 48

4034 132 100 88 74 65 62 59 57 59 54 53 48 50 50 48

4035 129 97 83 73 69 65 60 59 53 37 54 53 45 47 51

4036 123 92 84 75 69 64 60 58 57 53 53 47 44 49 46

4037 135 101 84 87 66 62 57 57 56 52 52 50 47 48 48

4038 134 96 88 78 73 68 65 62 58 53 53 47 48 48 47

4039 123 97 75 85 72 67 62 62 61 59 59 54 54 54 50

4040 143 109 88 85 85 73 66 67 55 59 57 53 53 51 51

404 1 133 100 86 78 71 68 68 69 62 63 54 51 48 50 49

4042 125 96 85 77 72 66 62 61 58 56 53 46 47 46 46

4043 118 96 82 71 69 62 63 58 57 53 49 46 46 49 45

4044 125 93 82 72 68 66 64 64 58 56 55 50 47 48 47

4045 136 107 91 81 72 68 65 65 62 61 59 51 50 51 40

4046 127 96 85 74 70 68 64 60 54 55 53 47 47 45 43

4047 127 98 83 74 67 59 58 60 58 56 46 47 47 50 47

4048 136 99 91 83 76 68 68 64 59 40 53 49 50 49 45

4049 135 98 87 81 73 68 64 61 59 58 16 53 52 51 50

4050 133 99 89 83 79 69 67 65 60 59 54 53 53 54 50

4051 135 104 88 77 74 70 65 65 61 56 52 49 46 48 45

MEAN 130 98 85 76 70 66 63 61 57 54 5 2 49 48 49 47

S.D. 6.1 4.0 3.6 7.3 4.7 3.2 3.4 3.7 3.0 5.8 7.8 2.6 2.7 2.5 2.7

N 26 26 26 26 25 26 26 26 26 26 26 26 26 26 26

SF=Spilled Feeder11

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 308

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

1526 124 96 83 80 79 76 74 73 77 66 63

1527 121 97 86 80 8 0 73 72 69 66 63 64

1528 119 100 99 81 78 78 78 74 70 71 71

1529 120 98 91 82 81 83 80 74 69 65 65

1530 124 101 98 89 89 82 75 78 69 57 57

1531 115 96 89 83 88 82 80 77 68 69 66

1532 125 99 95 91 82 86 88 85 70 70 66

1533 132 106 96 86 83 79 80 75 67 63 65

1534 127 99 98 86 86 78 77 76 65 62 66

1535 139 107 97 86 84 80 78 78 68 63 62

1536 131 105 100 91 97 79 80 78 70 65 70

1537 141 105 95 87 86 82 79 76 71 66 70

1538 122 99 95 86 84 81 74 71 69 61 62

1539 136 105 89 82 82 78 71 68 65 60 59

1540 111 93 93 85 82 62 82 77 71 64 66 57 54 57

1541 140 110 101 92 87 IW 82 80 72 73 68

1542 132 106 101 88 83 79 77 75 70 70 61

1543 134 125 107 93 91 90 82 78 74 68 68

1544 137 113 108 95 96 85 77 80 71 63 67

1545 125 108 101 92 84 82 80 78 76 71 71

1546 140 118 100 96 82 81 78 83 76 74 71

1547 130 111 104 89 83 78 76 75 71 68 67

1548 132 105 97 86 79 76 71 72 66 64 64

1549 99 85 83 76 69 77 68 63 63 63 56 51 110

1550 128 105 102 89 83 81 77 74 72 69 69

1551 128 102 93 83 79 77 32 105 78 69 65

MEAN 127 104 96 87 84 80 76 77 70 66 65 57 54 54 110

S.D. 9.8 8.1 6.5 4.9 5.6 5.1 9.9 7.4 3.8 4.2 4.1 0.0 0.0 4.4 0.0

N 26 26 26 26 26 25 26 26 26 26 26 1 1 2 1

IW=Invalid Weight: Feed left greater than feed fed weight11

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 309

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

2526 135 100 91 86 78 81 74 72 74 64 64

2527 130 109 92 86 87 81 78 75 SF 69 61

2528 130 102 94 83 85 82 82 76 75 73 72

2529 125 94 87 82 78 80 76 73 70 68 61

2530 137 107 99 93 88 80 80 81 72 69 68

2531 130 101 89 82 81 80 74 69 67 65 59

2532 139 97 87 79 81 76 75 73 69 61 62

2533 129 98 92 85 84 73 69 69 69 70 60

2534 134 106 93 89 90 88 75 SF 68 72 68

2535 129 98 85 81 76 76 74 73 68 64 66

2536 123 96 86 78 80 73 72 69 70 65 61

2537 139 100 89 84 81 79 73 73 67 64 59

2538 144 117 101 89 85 79 78 72 70 63 53

2539 132 102 94 86 79 74 73 70 67 58 57

2540 138 110 90 82 81 75 70 69 68 67 63

2541 122 92 52 114 87 80 73 72 72 64 62

2542 142 100 87 82 80 71 71 66 67 63 65

2543 132 103 88 82 76 73 73 71 69 68 66 70

2544 139 114 106 104 100 SF 92 86 83 74 67 64 53 58

2545 143 110 101 92 79 84 80 79 74 72 67

2546 153 113 97 89 80 77 76 78 74 69 63

2547 135 105 91 82 77 72 74 70 66 65 63

2548 132 103 100 89 83 75 76 76 71 68 69

2549 136 94 91 82 82 72 71 71 69 65 66 57 53 54

2550 145 95 97 99 91 SF 90 88 85 80 82

2551 138 117 108 80 80 79 74 68 72 68 60

MEAN 135 103 92 87 83 78 76 74 71 67 64 64 53 56

S.D. 7.2 7.1 10.1 8.1 5.5 4.2 5.5 5.4 4.8 4.6 5.6 6.6 0.4 2.6

N 26 26 26 26 26 24 26 25 2 5 26 26 3 2 2

SF=Spilled Feeder11

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 310

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (G RAMS/KG/DAY)

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

3526 125 98 92 84 78 80 76 74 69 62 61 54 51 51

3527 125 102 94 83 85 80 76 76 73 65 63

3528 129 95 92 84 83 85 78 79 70 64 62

3529 124 102 87 71 72 72 72 65 70 63 64

3530 135 95 85 82 81 76 75 67 62 61 56 55 57 52

3531 133 102 91 83 80 79 76 74 69 64 66

3532 133 107 95 84 89 85 85 83 71 70 69

3533 134 105 97 81 88 83 82 82 77 64 65

3534 SF SF SF 91 77 77 85 81 61 74 69 57 13 0

3535 121 100 87 88 83 78 80 77 81 63 68

3536 130 105 89 85 89 84 82 82 71 94 73

3537 130 103 107 88 86 83 84 82 76 71 74

3538 134 103 99 89 83 84 82 73 76 73 67

3539 135 119 95 88 84 78 79 75 78 68 66

3540 133 100 92 85 81 79 81 78 69 64 60

3541 128 100 97 90 83 84 83 74 72 69 67

3542 133 104 93 90 84 74 83 82 79 69 66

3543 142 SF 86 86 82 74 72 70 70 63 61

3544 121 106 92 86 84 83 79 80 74 69 72

3545 132 SF 99 89 79 77 84 75 82 70 75

3546 127 SF 89 87 SF 76 77 75 68 63 62

3547 133 106 96 92 SF 77 80 75 71 65 64

3548 135 109 96 92 84 76 7 6 78 69 68 64

3549 127 109 103 88 85 78 80 73 69 67 64

3550 129 117 100 91 85 89 85 78 75 69 66

3551 150 115 101 91 87 85 80 81 80 70 65

MEAN 131 105 94 86 83 80 80 76 72 68 66 54 54 54 130

S.D. 6.2 6.3 5.6 4.4 4.0 4.2 3.9 4.6 5.3 6.4 4.4 0.4 4.6 3.2 0.0

N 25 22 25 26 24 26 26 26 26 26 26 2 2 3 1

SF=Spilled Feeder11

- JAN

- 2010 17:07

Huntingdon Life Sciences 00

- 4205 PAGE 311

APPENDIX M P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL FEED CONSUMPTION (GRAMS/KG/DAY)

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF STUDY

ANIMAL# 0 7 14 21 28 35 42 49 56 63 69 91 98 105 112

-----------------------------------------------------------------------------------------------------------------------------------

4526 125 95 86 79 78 77 77 71 66 65 65

4527 127 104 91 90 84 80 73 71 71 63 63

4528 126 94 85 76 78 75 76 70 66 64 65

4529 145 102 94 92 98 82 93 85 74 89 67

4530 129 99 92 83 54 77 77 78 74 65 64 59

4531 120 87 79 78 73 72 67 72 57 63 63

4532 120 94 86 79 82 78 74 75 72 69 67

4533 127 95 SF 76 81 79 72 73 69 98 64

4534 136 101 87 76 80 79 SF 77 74 67 68

4535 127 96 86 84 75 73 70 69 64 64 60

4536 124 103 91 81 77 73 73 72 66 60 63

4537 135 106 91 84 82 76 72 72 68 60 60

4538 145 114 97 85 86 79 78 82 74 76 65

4539 118 97 89 80 83 83 79 75 71 76 68

4540 138 109 100 80 90 78 70 72 73 65 63

4541 129 SF 108 77 83 77 75 75 77 72 70

4542 125 102 95 88 88 82 79 72 71 80 SF

4543 134 97 88 89 79 72 73 75 67 59 66 57 52 52

4544 134 104 91 88 80 78 75 74 71 63 61 58 58 57

4545 138 104 92 87 82 77 75 72 73 70 65

4546 126 97 86 80 76 7 5 72 69 65 66 56

4547 142 112 98 88 86 79 75 76 74 68 71

4548 136 106 92 84 82 73 75 75 69 90 67

4549 131 89 83 83 80 74 78 76 63 63 64

4550 130 104 82 80 80 83 75 70 73 70 62

4551 141 106 100 87 89 82 81 74 79 70 75 60 56 55

MEAN 131 101 91 83 81 77 75 74 70 70 65 59 56 55

S.D. 7.6 6.6 6.6 4.6 7.6 3.3 4.9 3.6 4.9 9.9 4.0 1.7 3.0 2.7

N 26 25 25 26 26 26 25 26 26 26 25 4 3 3

SF=Spilled Feeder

# Huntingdon Life Sciences 00

- 4205 Page 312

211

- DIPE

- 1G Final Report

Individual Gestation Feed Consumption

Preface Appendix N

No data is presented for the following animal at the stated intervals for the

following reasons:

Animal No. Gestation

Days Reason

2536 0- 4 through

14

- 20 and

0

- 20 Sacrificed for Humane Reasons on Gestation

Day 111

- JAN

- 2010 17:11

Huntingdon Life S ciences 00

- 4205 PAGE 313

APPENDIX N P 0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

1526 66 70 65 64 67

1527 67 72 68 63 67

1528 74 80 73 64 73

1529 73 74 72 63 71

1530 61 65 69 61 64

1531 78 73 76 62 72

1532 65 75 68 62 67

1533 73 73 72 67 71

1534x NP 69 68 76 57 68

1535 67 67 64 60 65

1536 81 78 78 65 75

1537 79 76 72 61 72

1538x NP 64 58 57 49 57

1539 71 80 68 62 70

1540xNNMD

1541 69 74 71 65 70

1542 71 76 71 65 71

1543 71 81 73 62 72

1544 77 75 76 59 72

1545 76 80 72 70 75

1546 79 79 77 69 76

1547 69 75 73 72 72

1548x NP 66 69 62 57 63

1549 TLL 68 71 65 61 66

1550 78 79 70 65 73

1551 69 71 66 66 68

MEAN 72 75 71 64 70

S.D. 5.3 4.3 3.9 3.4 3.3

N 22 22 22 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN11

- JAN

- 2010 17:11

Huntingdon Life Sciences 00

- 4205 PAGE 314

APPENDIX N P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

2526 78 80 76 60 74

2527 71 71 67 53 65

2528 79 79 73 63 73

2529 71 73 72 62 69

2530 75 75 71 62 71

2531 62 64 65 57 62

2532 63 71 70 61 66

2533 71 70 70 65 69

2534 63 72 70 63 67

2535 63 67 66 61 64

2536 HS

2537 61 68 69 58 64

2538 67 70 63 55 64

2539 67 63 68 64 65

2540 72 71 70 59 68

2541 81 76 71 61 72

2542 SF 72 73 67 71

2543xPNMD

2544xNNMD

2545 77 74 74 66 73

2546 75 88 81 60 76

2547 68 65 66 62 66

2548 78 84 76 66 76

2549xNNMD

2550 95 88 77 67 82

2551 74 74 66 59 68

MEAN 72 73 71 61 69

S. D. 8.2 7.0 4.4 3.8 4.9

N 21 22 22 22 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN

SF=S pilled Feeder11

- JAN

- 2010 17:11

Huntingdon Life Sciences 00

- 4205 PAGE 315

APPENDIX N P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

3526xNNMD

3527 81 79 72 59 73

3528 73 76 71 BF 74

3529 73 77 72 60 71

3530xNNMD

3531 72 69 66 52 65

3532 79 85 77 68 77

3533 72 71 70 59 68

3534 TLL 64 80 71 68 71

3535 71 70 67 59 67

3536 71 75 71 66 71

3537 85 96 77 60 79

3538 68 71 66 54 65

3539 75 76 70 59 70

3540 76 75 71 59 70

3541 73 77 72 56 70

3542 76 75 76 62 72

3543 72 70 66 62 68

3544 83 78 72 62 74

3545 83 87 71 64 76

3546 65 73 68 60 66

3547 74 71 76 61 71

3548 71 74 70 67 71

3549 72 71 65 60 67

3550 79 75 70 61 71

3551 77 78 74 68 74

MEAN 74 76 71 61 71

S.D. 5.3 6.2 3.5 4.3 3.7

N 24 24 24 23 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN

BF=Broken Feeder11

- JAN

- 2010 17:11

Huntingdon Life Sciences 00

- 4205 PAGE 316

APPENDIX N P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY ST UDY IN RATS

INDIVIDUAL GESTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF GESTATION

ANIMAL# 0

- 4 4

- 7 7

- 14 14

- 20 0

- 20

-----------------------------------------------------------------------------------------------------------------------------------

4526 79 73 72 60 71

4527 67 59 55 58 60

4528 73 66 67 60 66

4529 76 81 73 67 74

4530xPNMD

4531 72 74 57 60 66

4532 77 79 76 70 75

4533 67 69 67 66 67

4534 89 80 73 66 77

4535 65 74 70 57 66

4536 66 69 64 59 64

4537 74 82 75 60 73

4538 HS 77 80 76 63 74

4539 75 77 75 66 73

4540 76 75 66 61 70

4541 75 73 73 68 72

4542 69 72 68 65 68

4543xNNMD

4544xNNMD

4545 67 64 68 64 66

4546 66 72 69 56 66

4547 70 74 72 60 69

4548 75 68 76 60 70

4549 69 78 73 60 70

4550 69 71 71 66 69

4551x NP

MEAN 72 73 70 62 69

S.D. 5.8 5.9 5.5 3.9 4.1

N 22 22 22 22 22

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE

x=EXCLUDED FROM MEAN

# Huntingdon Life Scien ces 00

- 4205 Page 317

211

- DIPE

- 1G Final Report

Individual Maternal Lactation

Feed Consumption

Preface Appendix O

No data is presented for the following animals at the stated intervals for the

following reasons:

Animal No. Lactation

Days Reason

1549 1- 4 through

21

- 28 and

1

- 28 Total Litter Loss on Lactation Day 2, therefore

weekly feed consumption was resumed.

2536 1- 4 through

21

- 28 and

1- 28 Sacrificed for Humane Reasons on Gestation

Day 1.

3534 1- 4 through

21

- 28 and

1

- 28 Total Litter Loss on Lactation Day 1, therefore

weekly feed consumption was resumed.

4538 14

- 21

through

21

- 28 and

1

- 28 Sacrificed for Humane Reas ons on Lactation

Day 14.11

- JAN

- 2010 17:14

Huntingdon Life Sciences 00

- 4205 PAGE 318

APPENDIX 0 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TO XICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

1526 108 120 146 193 372 188

1527 98 116 143 194 371 184

1528 75 96 123 173 307 155

1529 97 97 129 200 353 175

1530 57 74 98 136 248 122

1531 87 114 140 165 337 168

1532 96 111 142 181 EF 132

1533 104 114 130 173 352 175

1534x NP

1535 41 IW1 135 164 306 162

1536 96 116 134 177 EF 13 1

1537 116 118 147 197 EF 144

1538x NP

1539 76 95 131 177 368 169

1541 106 119 145 181 353 181

1542 65 IW1 153 181 318 179

1543 101 114 139 185 142 136

1544 88 98 134 185 372 176

1545 104 116 146 187 372 185

1546 118 125 145 202 EF 147

1547 72 80 103 137 266 132

1548x NP

1549 TLL

1550 85 107 143 177 366 176

1551 91 117 138 175 314 167

MEAN 90 108 135 178 324 161

S.D. 19.6 14.1 13.7 17.3 60.4 20 .9

N 21 19 21 21 17 21

NP=NOT PREGNANT TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN

EF=Empty Feeder IW1=Invalid Weight: Water ac cess problem11

- JAN

- 2010 17:14

Huntingdon Life Sciences 00

- 4205 PAGE 319

APPENDIX 0 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATIO N

ANIMAL# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

2526 92 115 138 201 414 192

2527 114 112 139 190 364 184

2528 85 114 144 172 305 164

2529 94 117 137 187 329 173

2530 105 124 144 202 EF 144

2531 105 117 138 180 343 176

2532 78 98 138 195 348 172

2533 87 104 141 189 EF 130

2534 9 7 113 139 IW IW 116

2535 96 103 132 179 419 186

2536 HS

2537 61 72 88 118 190 106

2 538 69 91 104 145 294 141

2539 82 97 123 182 317 160

2540 106 124 140 186 387 189

2541 96 112 133 180 366 177

2542 84 110 132 180 EF 126

2543 PNMD 121 129 151 219 374 199

2545 79 101 130 164 332 161

2546 98 118 143 205 378 188

2547 98 128 151 177 EF 139

2548 118 125 158 200 EF 150

2550 105 117 151 189 250 163

2551 79 100 129 194 382 177

MEAN 93 111 136 183 341 161

S.D. 15.0 13.4 15.2 21.2 58.2 25.8

N 23 23 23 22 17 23

PNMD=PR EGNANT, NO MATING DATE HS=HUMANE SACRIFICE

EF=Empty Feeder IW=Invalid Weight: Feed left greater than feed fed weight11

- JAN

- 2010 17:14

Huntingdon Life Sciences 00

- 4205 PAGE 320

APPENDIX 0 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUMPTION

-- (GRAMS/KG/DAY)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

3527 99 115 128 172 316 166

3528 93 104 125 164 301 158

3529 123 117 147 198 377 192

3531 89 114 140 201 294 168

3532 105 131 147 204 334 184

3533 97 113 140 171 284 161

3534 TLL

3535 100 103 126 165 276 154

3536 105 128 155 205 332 185

3537 114 128 158 210 406 203

3538 73 94 126 176 317 157

3539 93 146 149 172 392 190

3540 100 92 147 193 EF 133

3541 105 125 145 208 387 194

3542 94 108 142 204 360 182

3543 104 118 141 206 355 185

3544 111 133 153 197 333 185

3545 105 115 148 188 368 185

3546 104 116 135 189 343 177

3547 99 117 140 212 363 186

3548 102 119 150 203 335 182

3549 112 114 152 207 369 191

3550 159 99 146 173 350 186

3551 102 123 148 158 370 180

MEAN 104 116 143 190 344 178

S.D. 15.6 12.8 9.4 17.3 35.3 16.3

N 23 23 23 23 22 23

TLL=TOTAL LITTER LOSS

EF=Empty Feeder11

- JAN

- 2010 17:14

Huntingdon Life Sciences 00

- 4205 PAGE 321

APPENDIX 0 P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATERNAL LACTATION FEED CONSUM PTION -- (GRAMS/KG/DAY)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF LACTATION

ANIMAL# 1

- 4 4

- 7 7

- 14 14

- 21 21

- 28 1

- 28

-----------------------------------------------------------------------------------------------------------------------------------

4526 92 115 150 186 353 179

4527 93 125 151 192 356 183

4528 96 109 133 172 322 166

4529 79 107 141 168 EF 124

4530 PNMD 107 113 146 IW2 398 191

4531 143 120 137 167 EF 142

4532 113 135 155 253 354 202

4533 89 101 125 162 250 146

4534 99 114 137 195 364 182

4535 92 104 125 1 80 293 159

4536 101 113 148 193 274 166

4537 86 98 138 191 355 174

4538 x HS 119 143 150 137

4539 86 105 121 172 271 151

4540 82 114 140 192 EF 132

4541 121 119 143 195 365 189

4542 93 117 143 201 367 184

4545 84 100 132 174 324 163

4546 82 92 127 165 332 160

454 7 82 EF 136 178 EF 132

4548 111 134 134 166 331 175

4549 113 123 146 213 399 199

4550 127 122 160 214 356 196

MEAN 99 11 3 140 187 337 16 8

S.D. 16.8 11.2 10.1 21.6 41.8 2 2.8

N 2 2 21 22 21 18 2 2

PNMD=PREGNANT, NO MATING DATE x=EXCLU DED FROM MEA N HS=HUMANE SACRIFICE

EF=Empty Feeder IW2=Invalid weight: Weighing error11

- JAN

- 2010 17:35

Huntingdon Life Sciences 00

- 4205 PAGE 322

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DA YS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

1526 /P E D D D /E M D D /E D D D /E D D D /E D D D 14 1 5 1 4 4.3

1527 D /E D D D /E E D D D /E M D D D D /E E D D /E 13 0 7 1 4 4.8

1528 D D /E E D D /E M D D /E D D D /E D D D /E D D 14 0 6 1 4 4.0

1529 /M D D /E D D D /E D D D /E D D D /E D D D /E E 14 0 6 1 5 3.8

1530 /E D D D /E M D D /E M D D /E E D D D /E E M D 11 0 7 3 4 4.3

1531 D /P E D D D /P E D D D /E D D D /P E D D D /E 13 3 5 0 4 4.8

1532 D D /E D D D /E M D D /E D /E D /E M D D /E D D 13 0 6 2 5 3.2

1533 D D /E D D D /E D D D /E D D D /E M D D /E D D 15 0 5 1 4 4.0

1534 /E E D D D /E D D D /E E E D /E D D /E E E E E 9 0 12 0 4 4.0

1535 D D /E D D D D /E E D D D /E E D D D /E E D D 14 0 7 0 3 5.0

1536 /E D D D /E M D D /E E D D D /E E D D /E D D /E 12 0 8 1 5 4.0

1537 D D D /E M D D /E M D D D D /E D D D /E M D D 14 0 4 3 3 4.7

1538 D D /E E D D D /E E M D D /E D D D /E M D D /E 12 0 7 2 4 4.5

1539 D /E D D D /E D D D /E D D D /E D D D /E D D /E 15 0 6 0 5 3.8

1540 D /E D D D /E D D D /E D D /E E D D D D /E E D 14 0 7 0 4 4.3

1541 D D /E M D D /E D D D /E D D D /E M D D /E D D 14 0 5 2 4 4.0

1542 D D /E D D D /E M D D /E D D D /E M D D /E M D 13 0 5 3 4 4.0

1543 D D D /E M D D /E D D D /E M D D /E D D D /E D 14 0 5 2 4 4.0

1544 /E D D D /E D D /E E D D D /E D D D /E M D D /E 13 0 7 1 5 4.0

1545 D D /E D D D /E D D D /E D D D /E M D D /E D D 15 0 5 1 4 4.0

1546 /E E M D D D /E M D D /P E D D D D /E D D D /P 12 2 5 2 4 5.0

1547 D D /E D D D /E D D D /E D D D /E D D /E E D D 15 0 6 0 4 3.8

1548 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0

1549 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

1550 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0

1551 D /E M D D /P E D D D /P E D D D /P E D D D /P 12 4 4 1 4 4.8

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE11

- JAN

- 2010 17:35

Huntin gdon Life Sciences 00

- 4205 PAGE 323

A PPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

2526 D D D /E M D D D D /E D D D /E E M D D /E E D 13 0 6 2 3 5.0

2527 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0

2528 D D D /E E D D D /E D D D D /P E D D D /E D D 15 1 5 0 3 5.0

2529 D /E M D D /E D D D /E D D D /E D D D /E D D D 15 0 5 1 4 4.0

2530 /E E D D D /E D D D /E D D /E E D D D /E M D D 13 0 7 1 4 4.3

2531 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0

2532 D D D /E M D D /E E D D D /P E D D D /P E M D 12 2 5 2 3 4.7

253 3 D D D /E M D D /E D D D /E M D D /E M D D /E M 12 0 5 4 4 4.0

2534 D /E M D D /E E D D D /E M D D /E M D D /E M D 11 0 6 4 4 4.3

2535 D /E E D D D /E E D D D D /E D D D /E D /E D /E 13 0 8 0 5 3.8

2536 /E D D D /E D D D /E M D D D /P E D D D /E D D 14 1 5 1 4 4.5

2537 D D /E E D D D /E E D D D D /E D D D /E D D /E 14 0 7 0 4 4.5

2538 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

2539 D D /E E D D D /E E D D D /E E D D D /E D D D 14 0 7 0 3 5.0

2540 /E D D D /E M D D /E D D D /E D D D /E M D D /E 13 0 6 2 5 4.0

2541 /E D D D /E M D D /E D D D /E D D D /E D D D /E 14 0 6 1 5 4.0

2542 D D /E D D D /E D D D /E D D D /E D D D /E M D 15 0 5 1 4 4.0

2543 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0

2544 D D /E M D D /E D D D /E D D D /E D D D /E M D 14 0 5 2 4 4.0

2545 D D D /E M D D /E D D D /E E D D D /E M D D /E 13 0 6 2 4 4.3

2546 /M D D /E D D D /E D D D /E M D D /E D D D /E D 14 0 5 2 5 3.8

2547 D /E E M D D /E D D D /E D D D /E D D D /E M D 13 0 6 2 4 4.3

2548 D D D D D /E D D D /E D D D /E D D D /E M D D 16 0 4 1 3 4.0

2549 /E D D D /E D D D /E D D D /E M D D /E M D D /E 13 0 6 2 5 4.0

2550 D D /E M D D /E D D D /E M D D /E M D D / E D D 13 0 5 3 4 4.0

2551 /E D D D D /E D D D /P E D D D /P E M D D /P E 12 3 5 1 4 4.8

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE11

- JAN

- 2010 17:35

Huntingdon Life Sciences 00

- 4205 PAGE 324

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

IND IVIDUAL ESTROUS CYCLE DATA

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

3526 D D D D /E M D D /E E D D D /P D D D /E D D D 15 1 4 1 3 4.3

3527 /E D D D /E D D D /E D D D /E D D D /E M D D D 15 0 5 1 4 4.0

3528 D D D /E D D D /E D D D /E D D D /E M D D /E E 14 0 6 1 4 4.0

3529 D D D /E D D D /E D D D /E M D D /E M D D /E D 14 0 5 2 4 4.0

3530 D D /E D D D /E D D D /E D D D /E D D D /E D D 16 0 5 0 4 4.0

3531 D D /E M D D /E D D D / E D D D /E M D D /E D D 14 0 5 2 4 4.0

3532 D D D /E D D D /E D D D /E D D D /E M D D /E D 15 0 5 1 4 4.0

3533 /E D D D /E M D D /E M D D /E M D D /E M D D /E 11 0 6 4 5 4.0

3534 D D D /E M D D /E D D D /E M D D /E D D D /E D 14 0 5 2 4 4.0

3535 D /E M D D /E D D /E E D D /E E D D D /E D D D 13 0 7 1 4 4.0

3536 D /E M D D /E D D D /E D D D D /E M D D /E D D 14 0 5 2 4 4.3

3537 D D / E D D D /E D D D /E D D D /E M D D /E M D 14 0 5 2 4 4.0

3538 D /E E D D /P E D D D /P E M D D /E M D D /P E 10 3 6 2 4 4.5

3539 /E D D D D /E M D D /E E M D D /P E M D D /P E 10 2 6 3 4 4.8

3540 D /E D D D /E D D D /E D D D /E D D D /E D D D 16 0 5 0 4 4.0

3541 D D /E D D D /E D D D /E D D D /E M D D /E D D 15 0 5 1 4 4.0

3542 /E D D D /E D D D /E D D D /E D D D /E M D D /E 14 0 6 1 5 4.0

3543 /E M D D /E D D D /E D D D /E D D D /E M D D /E 13 0 6 2 5 4.0

3544 D D D /E E D D D /E E D D D /E E D D D /E E D 13 0 8 0 3 5.0

3545 D D D /E E D D D /E E M D D /E E D D D /E D D 13 0 7 1 3 5.0

3546 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

3547 /M D D /E D D D D /E M D D /E D D D /E E D D D 14 0 5 2 4 4.0

3548 D /E M D D /E D D D /E M D D D /E E D D D /E M 12 0 6 3 4 4.5

3549 /E M D D /E M D D /E M D D /E D D D /E E D D /E 11 0 7 3 5 4.0

3550 D D D /E M D D /E D D D /E D D /E E D D D /E D 14 0 6 1 4 4.0

3551 /E D D D /E D D D /E D D D /E E D D /E M D D /E 13 0 7 1 5 4.0

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE11

- JAN

- 2010 17:35

Huntingdon Life Sciences 00

- 4205 PAGE 325

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE DATA

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (PRE

- COHABITATION PERIOD) NUMBER OF DAYS

IN STAGE

FEMALE# 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 D P E M NC MD E

-----------------------------------------------------------------------------------------------------------------------------------

4526 D D /E M D D /E M D D /E M D D /E M D D /E M D 11 0 5 5 4 4.0

4527 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

4528 D D /E M D D /E D D D /E M D D /E M D D /E D D 13 0 5 3 4 4.0

4529 /P E M D D /E D D D D /E M D D /E M D D D /E D 12 1 5 3 4 4.8

4530 D D D D /E M D D /E D D D /E D D D /E D D D /E 15 0 5 1 4 4.0

4531 /E E D D D /E D D D /E M D /E E D D D /E M D D 12 0 7 2 4 4.3

4532 D D D /E D D D /E M D D /E D D D /E D D D /E M 14 0 5 2 4 4.0

4533 D D D /E M D D /E D D D /E D D D /E M D D /E D 14 0 5 2 4 4.0

4534 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

4535 D D D /E D D D /E M D D /E D D D /E D D D /E D 15 0 5 1 4 4.0

4536 D D /E D D D /E D D D /E M D D /E D D D /E D D 15 0 5 1 4 4.0

4537 D D D /E D D D /E D D D /E D D D /E D D D /E D 16 0 5 0 4 4.0

4538 D /E M D D /E D D D /E M D D /E M D D /E D D D 13 0 5 3 4 4.0

4539 D D D /E M D D /E D D D /E M D D /E D D D /E D 14 0 5 2 4 4.0

4540 D D D D D /M D D D D D D D D D D D /E M D D 18 0 1 2 1 a 12.0 a

4541 /E D D D /E D D D /E D D D /E D D D /E D D D /E 15 0 6 0 5 4.0

4542 D D /E M D D /E D D D /E M D D /E M D D /E M D 12 0 5 4 4 4.0

4543 D /E D D D /E D D D /E D D D /E M D D /E M D D 14 0 5 2 4 4.0

4544 D D D D D D D D D /E D D D /E M P E E E E E 12 1 7 1 1 4.0

4545 / E D D D /E D D /E E D D D /E D D D /E D D D /E 14 0 7 0 5 4.0

4546 D D D /E M D D /E D D D /E M D D /E M D D /E D 13 0 5 3 4 4.0

4 547 D D /E M D D /E D D D /E D D D /E D D D /E D D 15 0 5 1 4 4.0

4548 D /E D D D /E D D D /E D D D /E D D D /E D D D 16 0 5 0 4 4.0

4549 D /E D D D /E D D D /E D D D /E M D D /E D D D 15 0 5 1 4 4.0

4550 D D D /E D D D /E D D D /E D D D /E M D D /E M 14 0 5 2 4 4.0

4551 D D D /E D D D /E D D D /E D D D /E M D D /E D 15 0 5 1 4 4.0

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG NC=NUMBER OF CYCLES MDE=MEAN# OF DAYS OF CYCLE

A PSEUDOPREGNANT, EXCLUDED FROM CALCULATION.11

- JAN

- 2010 17:36

Huntingdon Life Sciences 00

- 4205 PAGE 326

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

1526 E M D D C

1527 E C

1528 D C

1529 D D C

1530 D E E C

1531 D D D C

1532 D C

1533 D C

1534 S

1535 D C

1536 S

1537 S

1538 E D D C

1539 E C

1540 D D E E E D D D D D D D D D

1541 D C

1542 D C

1543 D D C

1544 M D D C

1545 D C

1546 S

1547 D C

1548 D C

1549 D D D C

1550 D C

1551 E S

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG11

- JAN

- 2010 17:36

Huntingdon Life Sciences 00

- 4205 PAGE 327

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP II 2000 MG/M3

---------------------------------------------------------------------------------------------------------------------------- -------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

2526 D D C

2527 D D C

2528 D S

2529 S

2530 S

2531 D D C

2532 D P C

2533 D D C

2534 D S

2535 M D D C

2536 D D D D D D D C

2537 D D C

2538 D D D C

2539 D D C

2540 M D D C

2541 M D D S

2542 D C

2543 D D E D D D D D D D D D D D

2544 D E D D D E D D D E D D D E

2545 D D D C

2546 D E C

2547 D S

2548 S

2549 D D D E D D D E D D D E D D

2550 D C

2551 D D D C

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG11

- JAN

- 2010 17:36

Huntingdon Life Sciences 00

- 4205 PAGE 328

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

I NHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

3526 D E D D D P E D D D D D D D

3527 D D D S

3528 D D C

3529 D D S

3530 D E D D D E D D D E D E D D

3531 D S

3532 D D S

3533 M D D C

3534 D D C

3535 S

3536 D S

3537 D E D D D S

3538 D D D P C

3539 D D D P C

3540 S

3541 D D S

354 2 D D D C

3543 D D D C

3544 D D S

3545 E S

3546 D D D S

3547 S

3548 D D D C

3549 D D P S

3550 D D S

3551 D D D S

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG11

- JAN

- 2010 17:36

Huntingdon Life Sciences 00

- 4205 PAGE 329

APPENDIX P P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL ESTROUS CYCLE AND MATING DATA

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF EVALUATION (COHABITATION PERIOD)

FEMALE# 70 71 72 73 74 75 76 77 78 79 80 81 82 83

-----------------------------------------------------------------------------------------------------------------------------------

4526 D S

452 7 M D D E D D D S

4528 D C

4529 D D S

4530 M D D E D D D D D D D D D D

4531 S

4532 D D C

4533 D D S

4534 M D D C

4535 D D S

4536 D S

4537 D D S

4538 S

4539 D D S

4540 P C

4541 M D D S

4542 D E D D D S

4543 E D D D E D D D E M D D E D

4544 D E E E E E E E E E E E E E

4545 D D D E S

4546 D D S

4547 D S

4548 S

4549 E D D D S

4550 D D S

4551 D D E D D D E D D D E D D D

-----------------------------------------------------------------------------------------------------------------------------------

D=DIESTRUS M=METESTRUS P=PROESTRUS E=ESTRUS S=SPERM C=COPULATORY PLUG11

- JAN

- 2010 17:38

Huntingdon Life Sciences 00

- 4205 PAGE 330

APPENDIX Q P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Alternate Date of Date of Date of

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

------------------------------------------------------------------------------------------------------------------ -----------------

1526 1026 + P 28

- JUL

- 02 2

- AUG

- 02 24

- AUG

- 02

1527 1027 + P 28

- JUL

- 02 30

- JUL -02 21

- AUG

- 02

1528 1028 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1529 1029 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

1530 1030 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

1531 1031 + P 28

- JUL

- 02 1 -A UG

- 02 22

- AUG

- 02

1532 1032 + P 28

- JUL

- 02 30

- JUL

- 02 20

- AUG

- 02

1533 1033 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1534 1034 + NP 28

- JUL

- 02 29

- JUL

- 02

1535 1035 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1536 1036 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

1537 1037 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

1538 1038 + NP 28

- JUL

- 02 1

- AUG

- 02

1539 1039 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1540 1040

- NNMD 28

- JUL

- 02

1541 1041 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1542 1042 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1543 1043 + P 28

- JUL

- 0 2 31

- JUL

- 02 22- AUG

- 02

1544 1044 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

1545 1045 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1546 1046 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

1547 1047 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1548 1048 + NP 28

- JUL

- 02 30

- JUL

- 02

1549 1049 + TLL 28

- JUL

- 02 1

- AUG

- 02 23 -A UG

- 02

1550 1050 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

1551 1051 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DAT E TLL=TOTAL LITTER LOSS P=PREGNANT11

- JAN

- 2010 17:38

Huntingdon Life Sciences 00

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APPENDIX Q P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Alternate Date of Date of Date of

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

2526 2026 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2527 2027 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2528 2028 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

2529 2029 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

2530 2030 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

2531 2031 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2532 2032 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2533 203 3 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2534 2034 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

2535 2 035 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

2536 2036 + HS 28

- JUL

- 02 5

- AUG

- 02

2537 2037 + P 28

- JUL

- 02 31

- JUL

- 02 23

- AUG

- 02

2538 2038 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

2539 2039 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2540 2040 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

2541 2041 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

2542 2042 + P 28

- JUL

- 0 2 30

- JUL

- 02 22

- AUG

- 02

2543 20 43

- PNMD 28

- JUL

- 02 26

- AUG

- 02

2544 2044

- NNMD 28

- JUL

- 02

2545 2045 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

2546 2046 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

2547 2047 + P 28

- JUL

- 02 30

- JUL

- 02 20

- AUG

- 02

2548 2048 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

2549 2049

- NNMD 28

- JUL

- 02

2550 2050 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

2551 2051 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positi ve - = Sperm/Plug Negative N = Not Mated

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE P=PREGNANT11

- JAN

- 2010 17:38

Huntingdon Life Sciences 00

- 4205 PAGE 332

APPENDIX Q P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNM ENTS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Alternate Date of Date of Date of

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

3526 3026

- NNMD 28

- JUL

- 02

3527 3027 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

3528 3028 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

3529 3029 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

3530 3030

- NNMD 28

- JUL

- 02

3531 3031 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

3532 3032 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

3533 3033 + P 28

- JUL

- 02 1

- AUG

- 02 22

- AUG

- 02

3534 3034 + TLL 28

- JUL

- 02 31

- JUL

- 02 23

- AUG

- 02

3535 3035 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

3536 3036 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

3537 3037 + P 28

- JUL

- 02 3

- AUG

- 02 25

- AUG

- 02

3538 3038 + P 28

- JUL

- 02 2

- AUG

- 02 24

- AUG

- 02

3539 3039 + P 28

- JUL

- 02 2

- AUG

- 02 24

- AUG

- 02

3540 3040 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

3541 3041 + P 28

- JUL

- 02 31

- JUL

- 02 21

- AUG

- 02

3542 3042 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

3543 3035 + P 29

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

3544 3044 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

3545 3045 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

3546 3046 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

3547 3047 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

3548 3048 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

3549 3049 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

3550 3050 + P 28

- JUL

- 02 31

- JUL

- 02 21

- AUG

- 02

3551 3051 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS P=PREGNANT11

- JAN

- 2010 17:38

Huntingdon Life Sciences 00

- 4205 PAGE 333

APPENDIX Q P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXIC ITY STUDY IN RATS

INDIVIDUAL MATING ASSIGNMENTS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Alternate Dat e of Date of Date of

Female No. Male No. Male No. Sperm/Plug Outcome Cohabitation Insemination Delivery

-----------------------------------------------------------------------------------------------------------------------------------

4526 4026 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

4527 4027 + P 28

- JUL

- 02 5

- AUG

- 02 27

- AUG

- 02

4528 4028 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

4529 4029 + P 28

- JUL

- 02 31

- JUL

- 02 21

- AUG

- 02

4530 4030

- PNMD 28

- JUL

- 02 22

- AUG

- 02

4531 4031 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

4532 4032 + P 28

- JUL

- 02 31

- JUL

- 02 23

- AUG

- 02

4533 4033 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

4534 4034 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

4535 4035 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

4536 4036 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

4537 4037 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

4538 4038 + HS 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

4539 4039 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

4540 4040 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

4541 4041 + P 28

- JUL

- 02 1

- AUG

- 02 23

- AUG

- 02

4542 4042 + P 28

- JUL

- 0 2 3

- AUG

- 02 26

- AUG

- 02

4543 4043

- NNMD 28

- JUL

- 02

4544 4044

- NNMD 28

- JUL

- 02

4545 4045 + P 28

- JUL

- 02 2

- AUG

- 02 23

- AUG

- 02

4546 4046 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

4547 4047 + P 28

- JUL

- 02 30

- JUL

- 02 21

- AUG

- 02

4548 4048 + P 28

- JUL

- 02 29

- JUL

- 02 20

- AUG

- 02

4549 4049 + P 28

- JUL

- 02 2

- AUG

- 02 24

- AUG

- 02

4550 4050 + P 28

- JUL

- 02 31

- JUL

- 02 22

- AUG

- 02

4551 4051

- NP 28

- JUL

- 02

-----------------------------------------------------------------------------------------------------------------------------------

+ = Sperm/Plug Positive

- = Sperm/Plug Negative N = Not Mated

NP=NOT PREGN ANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE P=PREGNANT22

- JAN

- 2010 12:28

Huntingdon Life Sciences 00

- 4205 PAGE 334

APPENDIX R P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

1526 12 0 12 4 8 4 8 4 6 4 6 4 6 4 6 12 22

1527 12 0 12 8 4 8 4 6 4 6 4 6 4 6 4 13 22

1528 9 0 9 5 4 5 3 5 3 5 2 5 2 5 2 10 22

1529 12 0 12 4 7 4 7 4 6 4 6 4 6 4 6 13 22

1530 5 0 5 2 3 2 3 2 3 2 3 2 3 2 3 5 22

1531 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 14 21

1532 13 0 13 9 4 9 4 6 4 6 4 6 4 6 4 12 21

1533 14 1 15 5 9 5 9 5 5 5 5 5 5 5 5 15 22

1534x NP

1535 11 0 11 5 5 5 5 5 5 4 5 4 5 4 5 12 22

1536 12 0 12 5 7 5 7 5 5 5 5 5 5 5 5 12 22

1537 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 16 22

1538x NP

1539 12 1 13 6 6 6 6 5 5 5 5 5 5 5 5 17 22

1540xNNMD

1541 15 0 15 7 8 7 7 5 5 5 5 5 5 5 5 17 22

1542 11 0 11 6 5 6 5 5 5 5 5 5 5 5 5 11 22

1543 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 17 22

1544 12 1 13 7 5 7 5 5 5 5 5 5 5 5 5 13 22

1545 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 15 22

1546 13 0 13 4 9 4 9 4 6 4 6 4 6 4 6 15 22

1547 6 0 6 4 2 4 2 4 2 4 2 4 2 4 2 6 22

1548x NP

1549 TLL 13 1 14 7 6 0 0 0 0 0 0 0 0 0 0 14 22

1550 12 0 12 8 3 8 3 7 3 7 3 7 3 7 3 12 22

1551 9 0 9 3 6 3 6 3 6 3 6 3 6 3 6 9 22

M EAN 11.9 0.2 12.1 6.0 5.8 6.0 5.7 4.8 4.7 4.7 4.6 4.7 4.6 4.7 4.6 12.7 21.9

S.D. 2.7 0.4 2.8 2.1 2.0 2.1 2.0 1.0 1.1 1.1 1.2 1.1 1.2 1.1 1.2 3.2 0.3

N 22 22 22 22 22 21 21 21 21 21 21 21 21 21 21 22 22

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDED FROM MEAN

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY

DAY 4 COLUMNS = PRECULLING22

- JAN

- 2010 12:28

Huntingdon Life Sciences 00

- 4205 PAGE 335

APPENDIX R P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

2526 16 0 16 12 4 12 4 6 4 6 4 6 4 6 4 18 22

2527 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 15 22

2528 16 0 16 7 9 7 9 5 5 5 5 5 5 5 5 16 22

2529 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 15 22

2530 13 1 14 6 7 6 7 5 5 5 5 5 5 5 5 14 22

2531 16 0 16 7 8 7 8 5 5 5 5 5 5 5 5 17 22

2532 13 0 13 9 4 9 4 6 4 6 4 6 4 6 4 15 22

2533 17 0 17 9 8 9 8 5 5 5 5 5 5 5 5 16 22

2534 14 0 14 9 5 9 5 5 5 5 5 5 5 5 5 14 22

2535 10 0 10 7 3 7 3 7 3 7 3 7 3 7 3 10 22

2536x HS 18

2537 4 0 4 3 1 3 1 3 1 3 1 3 1 3 1 7 23

2538 11 0 11 5 4 5 4 5 4 5 4 5 4 5 4 11 22

2539 14 0 14 5 9 5 9 5 5 5 5 5 5 5 5 14 22

2540 11 0 11 6 5 6 5 5 5 5 5 5 5 5 5 12 22

2541 14 0 14 5 9 5 9 5 5 5 5 5 5 5 5 16 22

2542 13 0 13 5 7 5 7 5 5 5 5 5 5 5 5 15 22

2543 PNMD 16 0 16 7 9 7 9 5 5 5 5 5 5 5 5 18 no day 0

2544xNNMD

254 5 12 0 12 5 7 4 7 4 6 4 6 4 6 4 6 13 22

2546 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 16 22

2547 12 0 12 4 8 4 8 4 6 4 6 4 6 4 6 12 21

2548 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 14 22

2549xNNMD

2550 11 1 12 3 8 3 8 3 7 3 7 3 7 3 7 1 5 22

2551 12 0 12 4 8 4 8 4 6 4 6 4 6 4 6 12 22

MEAN 13.0 0.1 13.1 6.5 6.3 6.5 6.3 4.9 4.8 4.9 4.8 4.9 4.8 4.9 4. 8 14.1 22.0

S.D. 2.7 0.3 2.7 2.2 2.2 2.3 2.2 0.9 1.2 0.9 1.2 0.9 1.2 0.9 1.2 2.6 0.3

N 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 23 22

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE x=EXCLUDED FROM MEAN

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY

DAY 4 COLUMNS = PRECULLING22

- JAN

- 2010 12:28

Huntingdon Life Sciences 00

- 4205 PAGE 336

APPENDIX R P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATIO N REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NU MBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

3526xNNMD

3527 16 0 16 10 6 10 6 5 5 5 5 5 5 5 5 16 22

3528 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 15 22

3529 15 0 15 6 8 6 8 5 5 5 5 5 5 5 5 16 22

3530xNNMD

3531 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 16 22

3532 12 0 12 7 5 7 5 5 5 5 5 5 5 5 5 13 22

3533 15 0 15 7 8 7 8 5 5 5 5 5 5 5 5 15 21

3534 TLL 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 3 23

3535 15 0 15 8 6 8 6 5 5 5 5 5 5 5 5 15 22

3536 13 0 13 8 5 8 5 5 5 5 5 5 5 5 5 14 22

3537 13 0 13 11 2 11 2 8 2 8 2 8 2 8 2 14 22

3538 12 0 12 7 5 7 5 5 5 5 5 5 4 5 4 14 22

3539 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 14 22

3540 13 0 13 7 6 7 6 5 5 5 5 5 4 5 4 13 22

3541 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 14 21

3542 10 0 10 6 4 6 4 6 4 6 4 6 4 6 4 10 22

3543 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 16 22

3544 13 0 13 6 7 6 7 5 5 5 5 5 5 5 5 14 22

3545 13 0 13 5 8 5 8 5 5 5 5 5 5 5 5 14 22

3546 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 16 22

3547 10 0 10 6 4 6 4 6 4 6 4 6 4 6 4 14 22

3548 11 0 11 5 6 5 6 5 5 5 5 5 5 5 5 17 22

3549 14 1 15 6 8 6 8 5 5 5 5 5 5 5 5 15 22

3550 16 1 17 8 8 8 8 5 5 5 5 5 5 5 5 17 21

3551 15 0 15 5 10 5 10 5 5 5 5 5 5 5 5 17 22

MEAN 13.0 0.1 13.1 6.9 6.5 6.9 6.5 5.2 4.8 5.2 4.8 5.2 4.7 5.2 4.7 14.3 21. 9

S.D. 2.9 0.3 2.9 1.6 2.0 1.6 2.0 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 2.9 0.4

N 24 24 24 23 23 23 23 23 23 23 23 23 23 23 23 24 24

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS x=EXCLUDE D FROM MEAN

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY

DAY 4 COLUMNS = PRECULLING22

- JAN

- 2010 12:28

Huntingdon Life Sciences 00

- 4205 PAGE 337

APPENDIX R P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL DELIVERY AND LITTER DATA

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LITTER DELIVERED NUMBER OF LIVE PUPS TOTAL DURATION OF

DAYS IMPLAN

- GESTATION

LIVE DEAD TOTAL 1 4 7 14 21 28 TATIONS (DAYS)

FEMALE# N N N M F M F M F M F M F M F N N

4526 13 0 13 7 6 7 6 5 5 5 5 5 5 5 5 13 22

4527 11 1 12 6 5 6 5 5 5 5 5 5 5 5 5 12 22

4528 15 0 15 9 6 9 6 5 5 5 5 5 5 5 5 16 22

4529 1 5 0 15 11 4 11 4 6 4 6 4 6 4 6 4 16 21

4530 PNMD 15 0 15 6 9 6 9 5 5 5 5 5 5 5 5 16 no day 0

4531 14 0 14 7 7 7 7 5 5 5 5 5 5 5 5 15 22

4532 14 0 14 8 6 8 6 5 5 5 5 5 5 5 5 14 23

4533 8 1 9 5 3 5 3 5 3 5 3 5 3 5 3 17 22

4534 10 2 12 7 3 7 3 7 3 7 3 7 3 7 3 13 22

4535 15 1 16 10 5 10 5 5 5 5 5 5 5 5 5 16 22

4536 14 0 14 6 8 6 8 5 5 5 5 5 5 5 5 17 2 2

4537 14 0 14 5 9 5 9 5 5 5 5 5 5 5 5 15 22

4538x HS 14 1 15 5 9 5 9 5 5 5 5 0 0 0 0 16 22

4539 9 0 9 3 5 2 5 2 5 2 5 2 5 2 5 16 22

4540 13 0 13 6 7 5 7 5 5 5 5 5 5 5 5 14 22

4541 12 0 12 7 5 7 5 5 5 5 5 5 5 5 5 13 22

4542 9 0 9 5 4 5 4 5 4 5 4 5 4 5 4 11 23

4543xNNMD

4544xNNMD

4545 11 0 11 7 4 7 4 6 4 6 4 6 4 6 4 13 21

4546 12 2 14 5 6 5 6 5 5 5 5 5 5 5 5 15 22

4547 15 0 15 10 5 10 5 5 5 5 5 5 5 5 5 16 22

4548 14 0 14 6 8 6 8 5 5 5 5 5 5 5 5 15 22

4549 12 0 12 6 6 6 6 5 5 5 5 5 5 5 5 12 22

4550 15 0 15 8 7 8 7 5 5 5 5 5 5 5 5 14 22

4551x NP

MEAN 12.7 0.3 13.0 6.8 5.8 6.7 5.8 5.0 4.7 5.0 4.7 5.0 4.7 5.0 4.7 14.5 22.0

S.D. 2.2 0.6 2.1 1.9 1.7 2.1 1.7 0.8 0.6 0.8 0.6 0.8 0.6 0.8 0.6 1.7 0.4

N 22 22 22 2 2 22 22 22 22 22 22 22 22 22 22 22 22 21

NP=NOT PREGNANT PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE

x=EXCLUDED FROM MEAN

DEAD = STILLBORN AND CANNIBALIZED PUPS ONLY DAY 4 COLUMNS = PRECULLING22

- JAN

- 2010 12:31

Huntingdon Life Sciences 00

- 4205 PAGE 338

APPENDIX S F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

1526 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4

1527 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

1528 MK28 MK28 MK28 MK28 MK28 FK28 FD 2 FD11 FK28

1529 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FK28 FM 1 FK28

1530 MK28 MK28 FK28 FK28 FK28

1531 MK28 MK28 MC 4 MK28 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FK28

1532 MK28 MC 4 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

1533 FS MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4

1535 MK28 MD 8 MK28 MK28 MK28 FD 1 FK28 FK28 FK28 FK28 FK28

1536 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FK28

1537 MC 4 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FC 4 FK28 FK28 FK28 FK28

1539 MS MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FC 4 FK28 FK28

1541 MK28 MK28 MK28 MC 4 MC 4 MK28 MK28 FK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FM 2

1542 MK28 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

1543 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4 FK28

1544 MS MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

1545 MC 4 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FC 4 FK28 FK28 FK28 FK28 FC 4

1546 MK28 FK28 MK28 M K28 MK28 FK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FC 4

1547 MK28 MK28 MK28 MK28 FK28 FK28

1549 FS MD 2 MD 2 MD 2 MD 2 MD 2 MD 2 MD 2 FD 2 FD 2 FD 2 FD 2 FD 2 FD 2

1550 MC 4 MK28 MM 1 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

1551 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCERTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED M

- MISSING K

- SCHED ULED SACRIFICE22

- JAN

- 2010 12:31

Huntingdon Life Sciences 00

- 4205 PAGE 339

APPENDIX S F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

2526 MC 4 MC 4 MK28 MC 4 MK28 MC 4 MK28 MC 4 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28

2527 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 FC 4 FK28 FK28 FK28 FK28 FK28

2528 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4 FC 4

2529 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

2530 FS MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

2531 MC 4 MK28 MK28 MK28 MK28 MC 4 MK28 FC 4 FK28 FC 4 FC 4 FK28 FK28 FM 1 FK 28 FK28

2532 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28

2533 MK28 MC 4 MC 4 MC 4 MK28 MK28 MK28 MC 4 MK28 FC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4

2534 MK28 MC 4 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

2535 MK28 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

2537 MK28 MK28 MK28 FK28

2538 MK28 MK28 MK28 MD 0 MK28 MK28 FK28 FM 1 FK28 FK28 FK28

2539 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FC 4 FC 4 FK28 FK28 FC 4

2540 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

2541 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4 FC 4 FC 4

2542 MD 0 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FC 4 FK28 FK28

2543 MC 4 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FC 4 FK28 FK28 FC 4

2545 MK28 MK28 MK28 MM 2 MK28 FK28 FK28 FK28 FK28 FC 4 FK28 FK28

2546 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FC 4 FK28

2547 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FK28

2548 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

2550 FS MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28 FK28 FK28

2551 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCERTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED M

- MISSING K

- SCHEDUL ED SACRIFICE22

- JAN

- 2010 12:31

Huntingdon Life Sciences 00

- 4205 PAGE 340

APPENDIX S F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

3527 MC 4 MC 4 MK28 MK28 MK28 MC 4 MK28 MC 4 MC 4 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

3528 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FC 4 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

3529 MM 1 MC 4 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FC 4 FK28 FK28 FK28 FK28 FC 4

3531 MC 4 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

3532 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 MK28

3533 MK28 MC 4 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FK28 FK28 FC 4 FC 4

3534 FD 1 FD 1

3535 MK28 MD 1 MK28 MK28 MC 4 MK28 MC 4 MK28 FK28 FK28 FK28 MC 4 FC 4 FK28 FK28

3536 MC 4 MK28 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

3537 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 MK28 MK28 MC 4 M K28 FK28 FK28

3538 MK28 MC 4 MC 4 MK28 MK28 MK28 MK28 FK28 FM17 FK28 FK28 FK28

3539 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FC 4 FC 4 FK28 FK28 FK28

3540 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 FK28 FK28 FK28 FC 4 FD16 FK28

3541 MK28 MK28 MK28 MK28 MK28 MC 4 MC 4 MC 4 FK28 FC 4 FK28 FK28 FK28 FK28

3542 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

3543 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FC 4 FK28 FC 4 FK28 FC 4

3544 M C 4 MK28 MK28 MK28 MK28 MK28 FK28 FC 4 FK28 FK28 FC 4 FK28 FK28

3545 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FC 4 FC 4 FK28

3546 MC 4 MC 4 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

3547 MK28 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

3548 MK28 MK28 MK28 MK28 MK28 FC 4 FK28 FK28 FK28 FK28 FK28

3549 FS MK28 MK28 MK28 MK28 MC 4 MK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28 FC 4

3550 FS MC 4 MK28 MK28 MC 4 MC 4 MK28 MK 28 MK28 FK28 FC 4 FC 4 FK28 FC 4 FK28 FK28 FK28

3551 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FC 4 FK28 FC 4 FC 4 FC 4 FK28 FK28 FC 4

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U

- UNCE RTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED M

- MISSING K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:31

Huntingdon Life Sciences 00

- 4205 PAGE 341

APPENDIX S F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP SEX AND STATUS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

PUP #

FEMALE# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

-----------------------------------------------------------------------------------------------------------------------------------

4526 MK28 MC 4 MK28 MK28 MC 4 MK28 MK28 FK28 FC 4 FK28 FK28 FK28 FK28

4527 FS MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FK28

4528 MC 4 MK28 MC 4 MC 4 MK28 MK 28 MK28 MC 4 MK28 FK28 FK28 FC 4 FK28 FK28 FK28

4529 MK28 MK28 MK28 MC 4 MK28 MK28 MK28 MC 4 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28

4530 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FC 4 FC 4 FC 4 FK28 FK28 FK28 FC 4

4531 MK28 MK28 MK28 MK28 MC 4 MK28 MC 4 FK28 FK28 FK28 FK28 FK28 FC 4 FC 4

4532 MC 4 MC 4 MK28 MK28 MC 4 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

4533 MS MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28

4534 MS FS MK28 MK28 MK28 MK28 MK2 8 MK28 MK28 FK28 FK28 FK28

4535 MS MK28 MK28 MK28 MC 4 MK28 MK28 MC 4 MC 4 MC 4 MC 4 FK28 FK28 FK28 FK28 FK28

4536 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FC 4 FK28 FC 4 FC 4

4537 MK28 MK28 MK28 MK28 MK28 FK28 F K28 FC 4 FK28 FK28 FC 4 FC 4 FC 4 FK28

4538 US MU14 MU14 MU14 MU14 MU14 FU14 FU14 FU14 FU14 FU14 FC 4 FC 4 FC 4 FC 4

4539 UM 0 MM 2 MK28 MK28 FK28 FK28 FK28 FK28 FK28

4540 MK28 MK28 MK28 MM 3 MK28 MK28 FK28 FK28 FK28 FC 4 FC 4 FK28 FK28

4541 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28

4542 MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

4545 MK28 MK28 MC 4 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28

4546 FS FD 0 FS MK28 MK28 MK28 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

4547 MC 4 MK28 MK28 MK28 MC 4 MC 4 MC 4 MK28 MK28 MC 4 FK28 FK28 FK28 FK28 FK28

4548 MK28 MC 4 MK28 MK28 MK28 MK28 FC 4 FK28 FC 4 FC 4 FK28 FK28 FK28 FK28

4549 MK28 MK28 MK28 MC 4 MK28 MK28 FK28 FK28 FK28 FK28 FK28 FC 4

4550 MC 4 MC 4 MC 4 MK28 MK28 MK28 MK28 MK28 FC 4 FC 4 FK28 FK28 FK28 FK28 FK28

SEX CODE PRECEDES PUP STATUS CODE. NUMERICAL VALUE INDICATES DAY OF LACTATION.

SEX CODES: M

- MALE F

- FEMALE U -U NCERTAIN

PUP STATUS CODES: A

- ALIVE S

- STILLBORN D

- DIED C

- CULLED M

- MISSING K

- SCHEDULED SACRIFICE U

- UNSCHEDULED SACRIFICE11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 342

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1526 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

1527 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LI MITS P P P P P P

1528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NO RMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 343

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LA CTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1528 PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 SWOLLEN PAW(S) P

PUP# 8 SCABS P

1529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMIT S P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 W ITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

1530 PUP # 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

1531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 344

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVI DUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATION S LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1531 PUP# 10 WITH IN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1532 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1533 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 345

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ON E- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-------------------------------------------------------------------------------------------------------------------------- ---------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

1536 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

1537 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LI MITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NOR MAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITH IN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1 -S LIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 346

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPROD UCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1537 PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

1539 PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN N ORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WI THIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 347

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDU AL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1541 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P

1542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

1543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 20 10 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 348

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1544 PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

1545 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMIT S P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

1546 PUP# 1 WITHIN NORMA L LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 349

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1546 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

1547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LI MITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NOR MAL LIMITS P P P P P P

1549 PUP# 2 WITHIN NORMAL LIMITS P

PUP# 3 WITHIN NORMAL LIMITS P

PUP# 4 WITHIN NORMAL LIMITS P

PUP# 5 WITHIN NORMAL LIMITS P

PUP# 6 WITHIN NORMAL LIMITS P

PUP# 7 WITHIN NORMAL LIMITS P

PUP# 8 WITHIN NORMAL LIMITS P

PUP# 9 WITHIN NORMAL LIMITS P

PUP# 10 WITHIN NORMAL LIMITS P

PUP# 11 WITHIN NORMAL LIMITS P

PUP# 12 WITHIN NORMAL LIMITS P

PUP# 13 WITHIN NORMAL LIMITS P

PUP# 14 WITHIN NORMAL LIMITS P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:43

Huntingdon Life Sciences 00

- 4205 PAGE 350

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

1550 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN N ORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WI THIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

1551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:45

Huntingdon Life Sciences 00

- 4205 PAGE 351

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2526 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WI THIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 SWOLLEN SNOUT P

PUP# 9 ULCERATION

- CERVICAL P

PUP# 9 SCABS P

PUP# 9 OPACITY

- UNILATERAL 3 P

2527 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:45

Huntingdon Life Sciences 00

- 4205 PAGE 352

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENE RATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROU P II 2000 MG/M3

----------------------------------------------------------------------------------------------------------------------------- ------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2527 PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

2528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

PUP# 16 WITHIN NORMAL LIMITS P P

2529 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LI MITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NOR MAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITH IN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 353

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2530 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

2531 PUP# 1 WITHIN NORMAL L IMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NO RMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 354

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2532 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL L IMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

2533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P

PUP# 17 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Hun tingdon Life Sciences 00

- 4205 PAGE 355

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2534 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

2535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

2537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRE SENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 356

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2538 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

2539 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LI MITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NOR MAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

2540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

-------------------------------------------------------------------------------------------------------------------------------- ---

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 357

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURI NG LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATIO N 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2540 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

2541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN N ORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WI THIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

2542 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 358

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2543 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P

2545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 359

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GEN ERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GRO UP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2546 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

2547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

2548 PUP# 1 WITHIN NORMAL L IMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NO RMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:44

Huntingdon Life Sciences 00

- 4205 PAGE 360

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

2548 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

2550 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN N ORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WI THIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

2551 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 361

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP C LINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3527 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN N ORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WI THIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P

3528 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

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- 4205 PAGE 362

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3529 PUP# 1 WITHIN NORMAL LIMITS P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

3531 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P

PUP# 5 DOME HEAD P P

PUP# 7 DOME HEAD P P

PUP# 11 DOME HEAD P P

PUP# 13 DOME HEAD P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 363

APPENDIX T F1 GENERATION

GASOL INE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3532 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

3533 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LI MITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NOR MAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITH IN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

P UP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

3534 PUP# 1 WITHIN NORMAL LIMITS P

PUP# 2 WITHIN NORMAL LIMITS P

-------------------------------------------------------------------------------------------------------------------- ---------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 364

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3535 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL L IMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NO RMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 1 0 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

3536 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 365

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3538 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

3539 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRE SENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 366

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3539 PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3541 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL L IMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NO RMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-------------------------------------------------------------------------------------------------------------------------------- ---

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 367

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURI NG LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATIO N 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3541 PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

3542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN N ORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WI THIN NORMAL LIMITS P P P P P P

3543 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 368

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUA L PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3544 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

3546 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 369

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GE NERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GR OUP III 10000 MG/M3

----------------------------------------------------------------------------------------------------------------------------- - -----

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3546 PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

3547 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

3548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN N ORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 370

APPENDIX T F1 GENERATION

GA SOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3549 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

3550 PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

PUP# 17 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:47

Huntingdon Life Sciences 00

- 4205 PAGE 371

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINIC AL OBSERVATIONS DURING LACTATION

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

3551 PUP# 1 WITHIN NORMAL LIMIT S P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

------------------------------------------------------------------------------------------------------------------------ -----------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:50

Huntingdon Life Sciences 00

- 4205 PAGE 372

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

D AY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4526 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN N ORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WI THIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

452 7 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

4528 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

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- 4205 PAGE 373

APP ENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

45 28 PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

4529 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

4530 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 374

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR C ONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4530 PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P

4531 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

4532 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

----------------------------------------------------------------------------------------------------------------------------- - -----

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 375

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INH ALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4532 PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

4533 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMI TS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMA L LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

4534 PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PU P# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

4535 PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 376

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4535 PUP# 10 WITHIN NORMAL LIMITS P P

P UP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

PUP# 16 WITHIN NORMAL LIMITS P P P P P P

4536 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

4537 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESE NT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 377

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4537 PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

4538 PUP# 2 WITHIN NORMAL LIMITS P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P

PUP# 12 WITHIN NORMAL LIMITS P P

PUP# 13 WITHIN NORMAL LIMITS P P

PUP# 14 WITHIN NORMAL LIMITS P P

PUP# 15 WITHIN NORMAL LIMITS P P

4539 PUP# 2 WITHIN NORMAL LIMITS P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIM ITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORM AL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

4540 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

P UP# 4 WITHIN NORMAL LIMITS P

-----------------------------------------------------------------------------------------------------------------------------------

COD E: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 378

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION R EPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4540 PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

4541 PUP# 1 WITHIN NORMAL L IMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NO RMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

4542 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 379

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4545 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WI THIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

4546 PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WITHIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P

4547 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P

PUP# 6 WITHIN NORMAL LIMITS P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JA N- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 380

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

------------------------------------------------------------------------------------------------------------------ -----------------

4547 PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

4548 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN NORMAL LIMITS P P P P P P

4549 PUP# 1 WITHIN NORMAL LIMITS P P P P P P

PUP# 2 WITHIN NORMAL LIMITS P P P P P P

PUP# 3 WITHIN NORMAL LIMITS P P P P P P

PUP# 4 WITHIN NORMAL LIMITS P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL L IMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NO RMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P P P P P

PUP# 10 WIT HIN NORMAL LIMITS P P P P P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:49

Huntingdon Life Sciences 00

- 4205 PAGE 381

APPENDIX T F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP CLINICAL OBSERVATIONS DURING LACTATION

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

DAY OF 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2

FEMALE# OBSERVATIONS LACTATION 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

-----------------------------------------------------------------------------------------------------------------------------------

4549 PUP# 12 WITHIN NORMAL LIMITS P P

4550 PUP# 1 WITHIN NORMAL LIMITS P P

PUP# 2 WITHIN NORMAL LIMITS P P

PUP# 3 WITHIN NORMAL LIMITS P P

PUP# 4 WITHIN NORMAL LIMITS P P P P P P

PUP# 5 WITHIN NORMAL LIMITS P P P P P P

PUP# 6 WITHIN NORMAL LIMITS P P P P P P

PUP# 7 WITHIN NORMAL LIMITS P P P P P P

PUP# 8 WITHIN NORMAL LIMITS P P P P P P

PUP# 9 WITHIN NORMAL LIMITS P P

PUP# 10 WITHIN NORMAL LIMITS P P

PUP# 11 WITHIN NORMAL LIMITS P P P P P P

PUP# 12 WITHIN NORMAL LIMITS P P P P P P

PUP# 13 WITHIN NORMAL LIMITS P P P P P P

PUP# 14 WITHIN N ORMAL LIMITS P P P P P P

PUP# 15 WITHIN NORMAL LIMITS P P P P P P

-----------------------------------------------------------------------------------------------------------------------------------

CODE: 1

- SLIGHT 2

- MODERATE 3

- MARKED P

- PRESENT11

- JAN

- 2010 17:54

Huntingdon Life Sciences 00

- 4205 PAGE 382

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 8.3 8.2 8.7 8.6 8.9 7.6 7.8 8.4 8.6 8.5 8.4 8.0 7.7

1527 8.0 8.6 8.0 7.9 8.1 7.5 7.4 8.6 8.3 8.4 7.3 7.7 8.3

1528 7.4 8.3 7.6 7.9 6.0 8.5 8.3 5.3 7.1 7.8

1529 7.6 7.7 7.5 8.0 8.1 7.1 7.7 7.4 7.2 7.4 7.1 M 7.9

1530 8.5 8.2 8.9 8.4 8.2 8.7

1531 5.9 6.2 6.2 6.3 6.3 6.2 5.8 6.1 6.0 5.5 5.9 5.3 5.5 5.6

1532 6.4 6.9 6.2 5.8 6.6 6.5 6.9 6.9 6.8 6.3 6.3 6.0 5.8 6.0

1533 7.5 S 7.4 7.7 7.6 7.7 8.3 7.6 6.7 7.3 7.0 7.7 7.7 7.3 7.6 7.0

1534 NOT PREGNANT

1535 8.3 8.4 8.8 8.1 8.0 8.5 D 8.7 7.8 8.4 8.3 8.2

1536 7.9 8.8 7.9 8.1 8. 3 8.3 7.8 8.0 7.9 6.9 6.8 7.1 8.3

1537 7.0 7.1 7.0 7.9 6.7 7.0 6.9 7.3 7.2 6.3 7.1 6.6 7.0 6.5 7.0 7.3 6.8

1538 NOT PREGNANT

1539 7.5 S 8.3 7.2 7.3 8.1 7.6 7.9 7.1 6.9 8.0 7.1 7.6 6.9

1540 NOT PREG., NO MATING DATE

1541 7.3 7.2 8.0 7.1 7.4 7.3 7.9 6.8 6.5 6.7 7.3 6.9 7.8 7.0 7.9 7.4

1542 7.5 8.2 7.7 7.8 7.7 7.7 7.5 7.8 6.7 7.2 7.1 7.2

1543 7.3 7.7 7.7 7.7 7.5 8.0 7.7 7.4 7.7 7.1 7.3 6.9 6.9 6.8 6.8 5.8

154 4 7.5 S 8.4 7.9 8.1 7.7 7.4 7.7 7.1 7.6 7.3 6.9 7.5 7.0

1545 6.5 7.0 6.5 7.0 5.9 6.3 6.6 7.4 6.4 6.4 6.8 6.9 6.2 6.4 5.4 5.6

1546 7.6 8.2 7.8 8.0 8.1 8.1 7.9 7.4 7.7 6.7 7.0 7.7 7.2 7.1

1547 8.4 8.6 8.8 8.5 8.0 8.5 7.9

1548 NOT PREGNANT

1549 5.2 S 5.2 5.3 5.6 4.8 5.3 5.6 6.1 5.0 5.1 4.7 4.8 4.9 4.8

1550 7.7 8.0 8.2 M 7.7 7.9 7.8 8.1 8.4 8.3 6.7 7.0 6.4

1551 7.4 8.0 7.6 7.7 7.1 7.0 7.1 7.6 7.4 7.0

MEAN 7.4

S.D. 0.81

N 22

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:54

Huntingdon Life Sciences 00

- 4205 PAGE 383

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 6.9 7.2 7.3 6.4 6.5 7.5 7.1 6.7 7.0 7.7 7.1 6.6 7.2 6.3 6.4 6.3 6.5

2527 6.3 6.8 6.5 5.6 7.0 7.0 6.9 6.5 6.6 6.2 6.2 5.4 6.1 5.6 5.7

2528 7.2 7.7 7.6 6.9 7.8 7.1 7.1 7.4 7.2 7.4 6.5 7.9 7.0 7.2 6.8 6.5 6.7

2529 7.4 7.9 7.6 7.5 7.5 7.5 7.6 7.4 7.9 7.1 5.9 7.4 7.4 7.1

2530 7.5 S 7.7 7.4 7.6 8.2 8.2 7.7 7.3 7.6 7.2 7.3 7.4 7.5 7.0

2531 6.8 7.0 7.0 7.2 6.8 6.8 6.7 7.1 7.0 6.7 6.9 6.7 6.3 6.4 M 6.9 6.2

2532 7.3 7.4 7.7 7.7 6.7 7.6 7.5 7.3 7.1 7.4 7.2 7.0 7.2 7.2

2533 7.0 7.0 7.8 7.9 7.1 6.9 7.4 6.9 6.3 6.9 7.4 6.8 6.7 6.0 6.9 6.5 7.2 7.1

2534 7.4 6.8 6.5 7.0 8.1 7.3 8.0 8.2 7.2 8.4 6.5 6.7 7.8 7.5 7.3

2535 7.4 7.0 6.9 7.3 7.9 8.2 7.7 7.3 7.9 7.4 6.8

2536 HUMANE SACRIFICE

2537 8.2 7.5 7.8 8.9 8.5

2538 8.1 8.2 8.5 8.2 D 8.7 8.0 7.4 M 7.8 8.2 8.2

2539 7.0 8.0 7.6 7.2 7.5 7.2 7.2 6.9 7.3 7.0 6.7 6.5 6.0 6.2 6.4

2540 7.7 7.3 7.8 8.0 8.2 7.4 7.7 8.2 7.7 7.2 7.5 7.4

2541 7.5 7.1 8.4 7.8 8.2 7.0 6.9 7.0 8.2 7.4 8.0 7.1 7.3 7.6 7.4

2542 7.2 D 7.1 7.7 7.6 7.0 8.0 6.9 6 .5 6.9 7.1 7.6 6.4 7.2

2543 6.8 7.1 7.2 7.3 7.2 7.2 5.7 7.0 7.1 6.5 6.0 6.6 7.0 7.2 6.8 6.5 7.0

2544 NOT PREG., NO MATING DATE

2545 6.7 6.9 7.0 7.1 5.7 6.9 6.3 7.0 7.2 7.1 6.1 7.3 6.2

2546 7.4 7.5 7.6 7.6 5.9 7.5 7.3 8.0 7.9 7.6 7.5 7.4 7.0 7.5 7.2

2547 6.6 7.1 6.8 7.2 6.4 6.1 6.6 6.4 6.6 6.5 6.5 6.3 6.6

2548 7.0 7.0 7.2 7.3 7 .3 7.9 7.4 6.9 7.4 7.4 6.6 7.2 4.9 6.5

2549 NOT PREG., NO MATING DATE

2550 6.8 S 5.0 9.1 5.4 6.4 7.7 7.0 6.5 6.2 8.0 7.8 6.1

2551 7.6 7.6 7.5 7.6 8.0 8.0 7.8 7.3 7.7 7.7 7.5 7.1 7.3

MEAN 7.2

S.D. 0.46

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:54

Huntingdon Life Sciences 00

- 4205 PAGE 384

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREG., NO MATING DATE

3527 6.7 6.7 7.1 6.7 6.9 6.3 6.6 7.4 7.0 7.3 7.1 6.2 6.1 6.5 6.5 6.7 6.5

3528 7.4 7.2 7.1 7.6 8.0 8.1 7.5 6.9 6.6 7.1 7.3 6.6 7.7 7.7 7.1 7.9

3529 6.9 M 7.1 7.3 6.7 7.1 6.8 7.3 7.0 6.9 7.2 6.8 7.1 6.8 5.4 6.4

3530 NOT PREG., NO MATING DATE

3531 6.8 7.1 6.7 6.5 7.2 7.2 7.3 6.6 7.4 6.0 6.9 6.0 6.7 7.1

3532 7.6 7.5 7.5 7.9 7.9 6.7 8.0 7.7 6.8 8.1 7.6 7.2 7.8

3533 6.1 6.3 6.1 5.8 6.2 6.2 6.6 6.1 6.1 6.1 6.1 5.8 5.9 5.5 6.0 6.5

3534 TOTAL LITTER LOSS

3535 6.3 6.8 D 6.3 7.1 7.1 6.9 7.0 6.4 5.9 6.5 6.0 5.2 6.1 5.8 5.7

3536 6.8 5.7 7.4 7.3 7.1 7.1 7.2 6.9 6.5 7.2 6.3 6.5 6.5 6.4

3537 7.1 7.4 7.1 7.1 6.9 7.3 7.6 6.9 7.4 7.1 7.0 7.0 6.3 6.9

3538 7.3 7.4 7.3 7.0 7.3 7.3 7.2 8.3 6.7 7.0 7.2 7.8 6.9

3539 6.7 7.1 6.7 6.6 7.0 6.8 7.0 6.5 6.5 6.4 6.4 6.5 6.9 6.8

3540 7.6 7.2 7.6 7.7 7.7 7.9 8.0 7.9 7.4 7.6 7.8 7.4 7.5 7.3

3541 7.3 7.3 7.5 8.3 6.1 7.9 8.3 7.6 8.1 4.0 7.6 6.8 7.5 7.6 7.6

3542 8.1 8.4 8.3 9.0 8.1 8.5 7.9 7.9 8.4 7.4 6.8

3543 6.5 7.2 7.0 6.4 7.1 5.6 4.5 6.8 6.7 6.6 6.9 6.0 6.5 5.7 6.6 7.2

3544 6.0 6.1 5.9 6.1 6.2 6.4 5.9 6.5 6.0 5.7 6.1 6.0 5.1 5.7

3545 7.3 7.3 8.1 7.6 7.4 7.9 6.6 6.9 7.3 6.4 8.3 7.3 7.3 7.0

3546 7.2 7.0 6.8 7.7 7.2 7.4 7.7 7.6 7.3 6.7 6.9 6.6 7.9 7.0 7.1

3547 7.1 8.0 7.5 6.8 7.3 7.2 7.5 7.1 6.7 6.7 6.4

3548 8.0 8.6 8.3 8.1 8.7 8.0 8.2 7.8 7.6 7.7 8.0 7.5

3549 7.9 S 8.5 8.1 8.3 8.1 7.5 8.0 6.8 7.5 7.9 8.1 8.5 7.7 7.5 7.8

3550 5.8 S 6.0 6.4 5.9 6.5 5.3 6.0 5.9 5.9 5.2 5.4 5.9 5.7 5.0 5.9 5.6 5.7

3551 7.5 7.3 7.8 7.8 7.9 7.9 8.0 7.3 7.5 7.7 7.2 7.1 7.3 7.5 7.2 7.3

MEAN 7.0

S.D. 0.63

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:54

Huntingdon Life Sciences 00

- 4205 PAGE 385

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERAT ION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 1

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 7.4 7.3 8.0 7.8 7.8 7.2 7.4 7.6 7.4 7.0 6.9 7.2 7.2 7.8

4527 7.8 S 8.1 7.6 8.3 7.7 7.4 8.2 7.4 7.7 7.7 7.8 8.0

4528 6.6 7.0 6.5 6.3 7.2 6.9 7.0 6.8 6.9 6.6 6.3 6.5 6.4 6.5 5.3 6.1

4529 6.5 6.3 6.5 6.8 7.2 6.0 6.3 6.4 6.4 6.5 6.8 6 .8 6.2 6.9 6.4 6.7

4530 6.1 6.2 5.9 5.9 6.7 6.0 6.3 6.0 5.9 6.1 6.3 5.9 6.0 6.9 5.9 6.0

4531 7.0 7.3 7.0 6.9 7.6 6.9 7.9 7.4 7.0 6.7 6.4 6.6 7.5 6.8 6.0

4532 7.5 7.6 7.1 8.1 7.3 7.8 8.6 8.8 8.1 6.8 7.7 6.5 6.5 7.5 6.8

4533 6.9 S 6.7 7.4 7.3 7.5 5.4 6.7 6.5 7.3

4534 7.9 S S 7.7 8.1 8.1 8.6 7.6 7.9 8.3 7.4 6.9 8.1

4535 7.3 S 7.5 7.5 7.2 7.2 7.5 6 .5 8.0 8.1 7.6 7.3 7.2 6.7 7.0 7.1 7.4

4536 7.5 6.9 7.4 10.5 7.3 7.4 7.7 6.9 7.3 6.7 7.5 8.0 7.4 7.0 6.7

4537 7.7 8.0 8.0 7.6 8.4 8.2 6.9 7.4 7.5 7.7 7.9 7.2 7.5 8.4 7.7

4538 7.3 S 7.3 7.4 7.5 7.9 7.2 7.2 6.9 7.6 6.5 7.0 6.3 7.3 8.1 7.4

4539 7.5 M 4.0 7.3 9.4 7.9 7.0 8.7 8.0 7.4

4540 7.5 7.9 7.7 7.7 7.7 7.7 7.2 7.4 8.2 7.2 7.6 7.3 7.0 7.3

4541 7.4 7.2 7.3 7 .2 7.5 7.9 7.6 7.8 6.8 7.1 7.3 7.2 7.5

4542 8.2 8.7 8.6 7.7 8.6 7.6 8.0 8.3 8.2 8.2

4543 NOT PREG., NO M ATING DATE

4544 NOT PREG., NO MATING DATE

4545 7.6 7.7 7.8 8.2 7.9 7.9 7.9 7.8 7.5 6.4 7.4 6.7

4546 7.2 S D S 7.1 8.2 7.5 7.4 7.7 6.9 6.9 6.7 6.9 7.6 6.4

4547 7.3 7.6 7.5 7.8 7.6 7.2 8.3 7.3 7.0 7.6 7.4 7.2 6.7 7.1 6.3 6.6

4548 6.8 6.7 6.7 6.4 7.0 7.0 7.2 6.7 7.0 6.7 6.7 6.6 6.5 6.5 7.2

4549 7.2 7.4 7.8 6.6 7.4 7.4 7.1 7.5 6.8 6.7 7.2 7.1 7.2

4550 6.8 6.9 7.4 7.0 6.7 7.1 6.6 6.5 7.2 6.9 7.2 6.6 6.8 6.3 6.8 6.5

4551 NOT PREGNANT

MEAN 7.3

S.D. 0.49

N 22

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:55

Huntingdon Life Sciences 00

- 4205 PAGE 386

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 1 1.1 11.3 11.6 11.2 11.9 10.0 10.4 11.6 11.5 11.3 10.9 10.6 10.3

1527 12.1 12.6 12.3 12.1 12.0 11.8 11.2 12.5 12.7 12.3 10.9 12.0 12.4

1528 11.8 13.0 12.5 12.8 8.0 13.5 13.3 D 8.8 12.4

1529 10.9 11.0 10.7 11.7 11.2 10.3 11.1 10.8 10.7 11.3 10.7 M 10.6

1530 14.1 14.3 14.7 14.3 13.7 13.5

1531 8.2 8.6 8.2 8.6 8.9 8.6 8.4 8.4 8.4 8.1 7.9 6.9 7.9 7.9

1532 9.6 10.2 9.6 9.0 9.6 10.1 10.2 10.6 9.5 9.4 9.0 9.4 8.9 9.2

1533 11.6 S 12.1 11.3 12.3 11.7 12.4 11.6 10.7 11.7 10.8 11.7 11.3 11.7 11.5 10.9

1534 NOT PREGNANT

1535 12.0 11.7 12.7 11.5 11.8 12.6 D 12.2 10.9 12.4 12.1 12.4

1536 13.0 14.1 13.1 13.2 13.5 13.6 13.0 13.1 12.8 12.4 11.4 12.1 13.2

1537 10.3 10.7 11.0 11.2 9.7 11.2 10.0 10.3 10.4 10.0 10.9 9.6 10.3 10.3 9.3 9.9 9.9

1538 NOT PREGNANT

1539 12.2 S 13.5 12.2 12.0 12.6 12.1 13.0 12.0 11.4 12.0 11.4 12.3 11.4

1540 NOT PREG., NO MATING DATE

1541 11.4 12.0 12.5 12.0 11.5 12.2 12.0 10.8 11.0 10.3 11.9 9 .9 11.1 11.7 11.0 M

1542 11.3 11.9 12.2 11.6 11.6 11.4 10.7 11.4 10.3 11.0 11.4 10.6

1543 10.1 10.3 10.7 11.2 10.6 11.0 10.0 10.4 10.6 9.5 10.6 9.8 9.7 9.4 9.6 8.5

1544 11.3 S 12.2 11.7 11.9 11.9 11.5 11.0 10.7 11.0 11.2 10.1 11.1 11.3

1545 9.9 10.3 10.1 10.8 9.9 9.8 10.3 11.0 9.5 9.6 9.9 10.5 10.0 9.9 8.8 8.8

1546 11.0 12.1 10.9 10.9 11.2 12.0 11.0 10.4 11.5 10.2 10.9 11.0 10.8 10.0

1547 14.2 14.0 14.3 14.9 13.4 14.4 14.0

1548 NOT PREGNANT

1549 TOTAL LITTER LOSS

1550 11.8 12.0 12.8 M 12.5 12.5 11.8 12.9 12.2 11.9 11.1 11.0 9.5

1551 11.3 12.0 11.8 12.2 11.0 11.1 11.0 11.5 11.0 10.5

MEAN 11.4

S.D. 1.38

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:55

Huntingdon Life Sciences 00

- 4205 PAGE 387

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY ST UDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 9.4 9.4 10.4 9.1 9.9 9.4 9.7 8.9 8.7 10.4 9.9 9.6 9.9 9.1 8.0 9.1 8.5

2527 9.4 10.5 10.2 8.5 10.2 10.7 9.3 9.5 8.8 9.3 9.4 7.6 9.6 8.4 9.0

2528 8.9 8.8 8.6 8.3 9.8 9.7 9. 7 8.2 9.5 9.2 7.6 9.6 8.2 8.9 8.6 9.0 8.1

2529 10.3 10.7 10.3 10.5 10.5 10.7 10.7 10.3 10.9 9.8 8.5 10.5 9.9 10.1

2530 10.8 S 11.7 10.8 10.9 1 1.1 11.5 10.6 10.3 10.7 10.6 10.3 11.1 10.1 10.1

2531 9.7 10.2 10.0 10.1 9.6 9.4 9.4 10.1 9.2 9.4 9.4 10.0 9.7 9.3 M 10.7 9.5

2532 9.5 9.3 9.5 8.9 9.0 9.8 9.8 9.1 9.5 9.8 9.5 9.9 9.6 9.3

2533 9.6 9.6 11.2 9.9 9.5 9.6 9.0 10.1 9.1 9.5 10.5 9.8 10.3 8.3 9.6 9.1 9.0 9.7

2534 10.0 9.5 9. 5 9.2 11.3 10.2 10.0 11.0 10.2 11.2 9.4 9.3 10.3 10.0 9.5

2535 12.1 11.5 11.1 11.8 12.6 12.9 12.3 12.0 12.6 12.6 11.3

2536 HUMANE SAC RIFICE

2537 14.0 11.7 14.4 14.9 15.0

2538 12.6 12.6 12.9 13.1 D 13.2 12.6 11.5 M 12.2 12.4 12.6

2539 9.9 9.6 10.6 10.1 10.3 11.0 10.3 9.8 10.9 10.0 8.7 9.8 9.1 9.0 9.5

2540 11.8 11.3 11.9 12.1 12.4 12.0 11.7 12.5 11.9 11.1 11.5 11.4

2541 10.2 9.9 11.1 10.3 10.3 9.6 9.4 9.8 10.4 10.4 10.4 10.5 9.6 10.3 10.2

2542 10.2 D 10.8 11.2 10.7 9.7 11.1 10.0 9.4 9.9 9.8 10.7 9.4 10.2

2543 9.7 10.1 10.6 10.6 10.2 10.3 8.4 9.8 9.9 9.2 8.7 9.3 9.9 9.9 9.4 9.4 9.4

2544 NOT PREG., NO MATING DATE

2545 10.3 10.6 10.5 10.5 M 10.9 9.5 10.4 10.9 10.1 9.3 11.1 9.2

2546 10.5 11.0 10.8 9.8 7.2 10.6 11.0 12.6 10.3 10.4 11.4 10.3 10.0 10.4 10.5

2547 9.9 10.5 10.0 10.8 9.5 9.7 10.0 9.8 9.6 9.7 10.2 9.4 10.1

2548 10.6 10.3 11.2 11.4 10.9 10.9 11.4 10.6 11.1 10.8 10.5 11.0 7.6 9.7

2549 NOT PREG., NO MATING DATE

2550 9.9 S 6.2 12.4 7.6 9.6 11.3 10.7 9.9 9.3 10.9 11.6 9.1

2551 11.0 11.4 11.0 11.0 11.4 11.6 11.1 11.3 10.9 11.2 10.8 10.4 10. 4

MEAN 10.4

S.D. 1.18

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:55

Hunti ngdon Life Sciences 00

- 4205 PAGE 388

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREG., NO MATING DATE

3527 9.7 9.3 10.1 9.7 10.3 9.6 10.1 10.1 9.5 10.4 10.9 8.6 8.9 9.2 10.3 9.7 8.8

3528 10.9 10.7 10.4 11.9 11.5 11.8 11.4 10.2 10.2 10.6 10.9 9.7 10.9 11.0 11 .2 11.6

3529 10.3 M 10.5 9.9 10.4 10.1 11.3 11.1 11.1 9.8 11.2 10.2 10.0 10.2 8.8 10.0

3530 NOT PREG., NO MATING DATE

3531 9.8 10.4 10.1 9.8 11.0 8.5 10.6 9.3 10.6 8.8 10.2 9.1 9.9 9.7

3532 11.4 11.6 11.3 12.2 11.8 9.9 11.8 11.5 10.3 11.8 11.1 11.7 11.5

3533 8.8 9.2 8.8 9.1 9.3 8.7 9.5 8.8 9.0 8.6 9.0 8.0 8.0 8.1 8.2 9.3

3534 TOTAL LITTER LOSS

3535 9.4 9.8 D 9.8 9.8 10.4 11.1 9.8 9.8 8.5 9.6 8.7 7.9 9.1 9.0 8.5

3536 9.5 8.4 9.7 10.4 10.0 9.9 9.8 9.9 9.5 9.1 8.9 9.0 9.7 9.7

3537 10.8 11.5 10.8 11.0 11.0 11.5 10.7 10.5 11.1 11.1 10.1 10.9 10.4 10.4

3538 11.5 11.7 11.8 11.0 12.0 11.0 11.7 12.4 10.4 11.4 11.6 12.2 10.8

3539 9.7 10.1 9.8 8.5 10.1 10.0 10.8 9.8 9.4 9.2 9.6 9.7 10.1 8.4

3540 11.2 10.7 12.1 12.0 11.7 11.6 11 .3 11.5 10.7 10.9 11.2 10.7 10.0 10.8

3541 11.4 11.4 11.3 12.4 10.2 12.6 13.0 12.8 11.9 6.7 11.7 10.7 11.2 11.4 11.9

3542 12.7 13.0 13.1 13.5 12.8 13.1 12.5 12.7 12.8 12.2 11.4

3543 9.0 9.9 10.3 9.2 10.5 7.7 5.9 9.3 9.0 8.5 9.3 8.3 9.2 8.6 8.6 10.1

3544 8.3 8.4 8.3 8.3 8.7 8.9 8.8 8.9 7.4 8.0 8.7 8.3 7.3 8.2

3545 10.4 11.3 10.9 10.7 11.0 11.3 9.2 9.8 11.0 9.0 10.3 10.5 10.4 10.0

3546 10.2 10.1 9 .7 11.1 10.1 10.7 10.3 10.9 10.3 9.7 9.8 9.5 10.8 10.2 9.9

3547 10.6 11.8 11.0 10.1 10.8 10.8 11.1 10.5 10.2 10.3 9.6

3548 11.9 12.6 12.6 11.7 13.0 11.7 11.2 11.8 11.5 11.3 11.8 11.3

3549 10.2 S 10.3 9.3 10.5 10.9 10.0 10.5 9.1 9.7 10.8 10.5 10.7 9.3 10.5 10.4

3550 7.9 S 7.9 8.1 8.5 8.6 7.7 8.4 7.7 8.0 7.3 7.3 8.0 8.1 7.3 8.0 8.0 7.1

3551 10.1 10.3 10.4 11.0 10.4 10.3 9.7 10.1 9.6 10.7 9.8 9.8 10.5 9.2 10.2 9.3

M EAN 10.2

S.D. 1.16

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:55

Huntingdon Life Sciences 00

- 4205 PAGE 389

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGH TS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 4

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 10.1 9.9 10.7 9.9 10.6 9.7 10.4 10.9 10.0 9.0 9.8 10.0 10.1 10.4

4527 10.8 S 11.2 11.3 11.4 10.5 10.9 11.0 10.0 10.3 10.7 10.3 11.0

4528 9.5 10.3 9.4 9.3 9.9 9.7 10.2 10.2 9.7 9.7 9.0 9.5 9.2 9.9 7.6 9.4

4529 9 .2 8.8 9.8 9.2 9.0 9.1 9.3 9.0 9.0 9.3 9.5 9.8 8.6 9.6 9.0 9.3

4530 8.6 8.6 8.4 8.7 9.8 8.6 8.6 8.7 8.2 8.5 7.9 8.2 8.4 9.2 8.4 8.7

4 531 10.4 11.3 10.3 10.5 10.9 10.0 10.9 11.0 10.8 9.3 9.6 10.2 11.0 9.7 10.0

4532 10.6 10.6 10.9 10.9 10.6 10.4 11.3 12.2 11.1 9.8 10.9 10.0 9.3 11.2 9.8

4533 10.2 S 10.3 11.2 10.5 9.9 8.4 10.3 10.4 10.8

4534 11.6 S S 10.8 12.3 11.4 12.1 11.5 11.3 12.0 11.7 10.3 12.3

4535 9.8 S 9.9 10.1 9.7 9.7 10.0 8.8 10.5 10.9 10.1 9.6 9.4 9.0 9.3 9.9 10.3

4536 10.6 10.1 10.9 13.6 10.8 10.6 10.3 10.7 10.3 9.9 10.5 10.5 10.2 10.4 9.9

4537 10.7 11.0 11.0 9.7 10.8 11.6 10.5 10.8 10.6 10.3 10.9 10.4 10.8 11.4 10.6

4538 10.9 S 10.9 11.0 11.2 11.4 11.5 10.2 10.4 11.3 10.3 10.6 10.0 11.6 11.2 10. 7

4539 12.1 M M 11.7 13.8 12.3 10.0 13.0 12.5 11.4

4540 11.3 12.2 11.6 11.8 M 10.7 11.2 11.2 11.8 11.1 11.7 11.2 10.2 11.0

4541 11.3 11.7 11.1 10.9 11.9 11.8 11.9 11.8 10.7 10.8 10.9 11.2 11.1

4542 12.0 12.9 12.5 11.0 13.0 11.3 11.2 12.1 11.4 12.7

4543 NOT PREG., NO MATING DATE

4544 NOT PREG., NO MATING DATE

4545 11.2 11.7 11.5 12.0 11.7 11.2 11.3 11.4 11.0 10.0 11.4 9.8

4546 10.6 S D S 10.6 11.5 10.9 11.0 11.1 10.0 10.3 9.9 10.1 10.9 10.2

4547 9.9 11.0 10.8 10.5 9.5 9.7 10.9 10.4 9.9 9.9 10.2 9.1 9.0 9.5 9.0 9.7

4548 9.2 8.2 9.7 9.1 8.7 9.3 9.0 9.2 9.1 9.6 9.4 9.4 9.3 9.0 10.1

4549 11.6 12.0 12.6 11.7 11.9 11.8 12.1 11.6 10.8 10.7 11.7 11.2 11.2

4550 9.5 9.4 9.7 10.0 9.7 9.6 9.7 8.8 9.6 10.1 10.2 9.3 9.7 8.2 10.0 9.2

4551 NOT PREGNANT

MEAN 10.5

S.D. 0.95

N 22

PUP STATUS CODES: S

- STILLBORN D

- DIED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 390

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDEN SATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 7

---------------------------------------------------------------------------------------------------------------- -------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 13.7 13.6 14.2 13.7 15.0 12.5 13.4 13.9 C 13.9 13.6 13.1 C

1527 15.8 C 16.1 C 15.6 15.7 15.3 17.0 16.5 15. 5 14.6 15.3 15.9

1528 14.4 16.6 16.3 16.0 7.3 16.9 16.8 D 9.6 16.0

1529 14.6 15.3 14.4 15.6 14.8 13.3 15.2 13.7 1 4.5 C 14.1 M 15.0

1530 18.9 19.4 19.1 18.9 18.7 18.4

1531 10.7 11.3 10.8 C 11.2 11.6 C C 10.5 11.0 10.6 9.1 10.0 10.5

1532 14.6 15.2 C C 14.4 C 15.4 16.1 14.6 14.5 14.0 14.4 13.7 13.9

1533 15.3 S 16.3 14.8 16.4 15.1 16.2 C 14.2 14.6 C 15.2 15.2 15.0 C C

1534 NOT PREGNANT

1535 10.0 9.7 11.1 9.4 9.7 10.7 D 9.8 9.1 10.7 9.7 10.4

1536 17.1 18.7 17.2 17.0 17.3 17.9 17.2 C 17.3 15.9 C 15.9 17.0

1537 15.6 C C C 15.4 17.3 15.8 15.7 15.7 C C 15.1 C 15.6 14.4 15.3 15.6

1538 NOT PREGNANT

1539 15.7 S C 15.6 16.1 16.8 15.7 17.0 15.5 14.5 15.2 C 16.2 14.6

1540 NOT PREG., NO MATING DATE

1541 14 .7 15.1 16.1 15.3 C C 15.9 14.3 14.1 C C 13.2 14.1 14.9 14.0 M

1542 10.4 10.7 11.2 10.6 10.6 10.9 C 10.4 9.4 9.8 10.2 9.8

1 543 14.1 14.9 14.9 14.9 14.5 C 14.2 C 14.5 13.5 C C 13.9 13.9 C 11.9

1544 15.5 S C C 16.1 16.6 15.7 15.5 15.3 15.5 14.9 14.1 15.7 15.5

1545 12.7 C 12.7 13.7 13.1 12.6 13.1 C C 11.8 C 13.6 13.4 12.3 10.9 C

1546 15.9 16.8 15.9 15.7 16.4 16.8 16.2 15.0 C 15.0 16.0 C 15.5 C

1547 17.9 17.6 18.6 18.3 16.7 18.0 18.2

1548 NOT PREGNANT

1549 TOTAL LITTER LOSS

1550 14.3 C 15.2 M 14.6 14.9 14.5 14.9 14.7 14.5 13.7 13.5 12.0

1551 14.3 15.4 15.1 15.2 13.7 13.8 13.7 14.5 13.6 13.5

MEAN 14.6

S.D. 2.25

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 391

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 12.7 C C 12.8 C 13.1 C 11.9 C 14.4 13.5 13.4 C 12.4 11.2 12.1 12.5

2527 13.2 13.8 14.3 12.5 14.9 C 13.7 C C C 12.8 11.4 13.7 12.3 12.9

2528 12.2 12.2 11.8 12.1 13.4 13.2 C C 13.3 11.8 10.9 C C 11.7 11.9 C C

2529 13.8 C 13.8 C 14.3 14.4 14.4 14.0 C 11.8 13.5 13.9 13.9 13.7

2530 16.0 S 17.0 16.5 16.1 C 17.0 15.6 14.5 16.7 C C 16.1 15.1 15.6

2531 14.5 C 14.6 14.9 15.0 14.4 C 15.2 C 13.3 C C 14.2 13.6 M 15.2 14.3

2532 13.3 13.0 13.8 12.7 12.6 C 13.6 C C 13.4 13.5 14.0 13.1 12.8

2533 14.8 14.7 C C C 15.1 14.4 16.5 C 15.3 C 14.9 15.0 12. 9 14.8 14.1 C C

2534 14.1 13.7 C C 15.1 C C 15.7 13.4 15.3 13.7 12.6 13.9 13.8 13.7

2535 16.0 15.6 15.1 15.7 17.3 16.8 15.7 15.8 16.6 16.8 14.8

2536 HUMANE SACRIFICE

2537 18.6 15.0 19.4 20.0 19.8

2538 15.8 15.6 15.6 16.8 D 16.9 15.9 14.0 M 15.8 15.8 15.7

2539 14.7 14.2 14.9 14.3 14.9 15.8 14.7 14.8 C 15. 4 C C 13.7 13.8 C

2540 15.3 14.9 15.6 C 16.2 15.9 15.6 15.7 15.2 14.3 14.9 14.6

2541 13.6 13.9 14.7 14.3 13.6 13.4 12.9 13.0 13.6 12.8 13.6 C C C C

2542 14.5 D 14.5 19.0 14.9 13.7 14.9 C 13.4 13.6 13.8 C 13.3 14.3

2543 15.1 C 16.3 16.4 16.2 C 12.3 15.5 15.4 14.2 C C 15.8 C 14.6 14.4 C

2544 NOT PREG., NO MATING DATE

2545 14.1 14.1 14.8 14.3 M 14. 8 13.2 14.8 14.6 13.8 C 14.6 12.5

2546 15.1 15.9 15.7 13.8 C 15.6 C C 14.9 15.0 16.6 14.9 13.4 C 15.3

2547 14.3 15.1 13.7 15.6 1 3.4 14.1 14.0 C 13.8 14.3 14.6 C 14.5

2548 15.1 15.2 16.3 16.3 15.5 C 16.5 C C 15.2 14.8 15.4 11.3 14.2

2549 NOT PREG., NO MATING DATE

2550 13.0 S 8.2 16.2 10.0 12.9 C 14.3 13.0 12.3 15.2 15.2 12.4

2551 15.5 16. 0 16.0 15.5 15.8 15.3 15.7 C C 15.5 15.1 14.7 15.1

MEAN 14.6

S.D. 1.36

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 392

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPROD UCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREG., NO MATING DATE

3527 13.7 C C 13.5 14.9 14.0 C 13.9 C C 14.9 12.5 12.6 13.1 14.3 13.2 C

3528 15.9 15.5 14.9 16. 8 16.3 17.0 C C C C C 14.1 16.4 16.3 15.9 16.3

3529 14.7 M C 14.5 15.5 14.2 15.8 15.9 C 14.1 C 14.6 15.0 14.5 12.8 C

3530 NOT PREG., NO MA TING DATE

3531 13.6 C 14.5 14.2 15.4 12.5 C 13.3 C 12.6 14.0 12.2 13.8 13.6

3532 15.4 C C 16.2 16.0 13.2 15.8 15.6 14.5 16.3 14.8 15.3 15.8

3533 12.8 13.5 C 13.1 C 12.8 13.8 12.9 12.8 12.9 C 12.1 11.7 12.2 C C

3534 TOTAL LITTER LOSS

3535 12.5 12.8 D 11.8 13.6 C 13.6 C 13.0 11.4 13.1 11.7 C C 12.8 11.5

3536 12.9 C 13.2 C C 13.5 13.5 13.2 13.2 12.6 12.1 12.7 12.9 12.5

3537 15.8 16.4 15.6 16.1 15.6 C 15.9 C 16.4 16.5 C 15.4 15.3 14.9

3538 15.2 15.7 C C 15.4 14.8 15.2 16.5 13.8 14.3 15.5 15.9 14.5

3539 13.6 13.7 13.6 11.6 14.6 14.3 C 14.8 13.0 C C 13.7 14.2 12.5

3540 15.2 15.4 16.1 16.3 15.9 14.6 C C 14.7 15.2 15.5 C 13.3 15.3

3541 16.7 18.4 17.2 18.8 15.4 18.5 C C C 10.4 C 16.1 17.2 17.5 17.8

3542 16.5 16.8 16.4 17.5 16.7 17.3 15.7 16.6 17.0 16.1 15.3

3543 13.5 14.0 14.5 13.5 14.6 11.9 C C 13.4 13.0 14.0 C 13.0 C 13.2 C

3544 11.6 C 11.4 11.8 12.0 12.5 12.0 12.2 C 10.8 11.9 C 9.5 12.1

3545 15.3 16.6 15.1 15.6 15.5 16.1 C 14.7 16.1 13.5 15.4 C C 14. 4

3546 14.3 C C 15.4 C 14.5 13.9 15.2 14.3 13.4 13.3 13.5 15.3 14.6 C

3547 13.7 14.9 14.1 13.1 14.8 14.0 14.0 13.7 13.1 13.5 12.2

3548 16.0 16.3 16.6 15.8 16.9 16.7 C 15.4 15.6 15.5 16.0 15.6

3549 14.3 S 14.8 14.4 15.2 14.3 C 14.4 13.0 13.9 14.9 C C 13.4 14.2 C

3550 11.1 S C 11.5 11.7 C C 11.5 11.2 11.3 10.8 C C 11.1 C 10.9 11.1 10.2

3551 13.8 13.4 13.9 14.6 14.0 14.1 13.4 14.0 C 14.4 C C C 13.0 13.3 C

MEAN 14.3

S.D. 1.51

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 20 10 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 393

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 7

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 13.6 13.5 C 13.6 14.2 C 13.4 14.1 13.3 C 13.5 12.8 13.7 14.1

4527 15.0 S 15.1 15.5 15.4 C 15.2 15.5 14.2 14.7 14.8 14.6 15.2

4528 13.2 C 13.1 C C 13.5 14.4 14.1 C 13.4 13.0 13. 2 C 13.2 11.1 13.2

4529 13.4 13.7 14.1 12.8 C 13.1 13.3 13.4 C C C C 13.2 12.6 14.2 13.6

4530 13.0 13.3 13.2 12.8 C 12.7 13.2 12.8 12.3 C C C 12.9 13.9 12.9 C

4531 14.1 14.9 13.5 14.3 15.3 C 15.2 C 13.8 12.9 12.8 14.1 14.7 C C

4532 14.0 C C 15.0 13.7 C 15.1 15.1 15.2 13.1 13.8 12.3 12.4 14.4 C

4533 13.2 S 13.3 14.0 13.6 12.9 11.0 13.4 13.5 13.6

4534 15.4 S S 14.7 15.5 14.8 16.1 15. 8 15.3 16.3 15.5 13.7 16.2

4535 13.3 S 13.7 13.9 13.3 C 13.3 12.1 C C C C 13.4 12.5 12.9 13.9 13.8

4536 15.9 C 16.7 18.4 16.2 16.4 1 5.5 15.1 15.1 15.1 15.4 C 15.4 C C

4537 15.3 16.3 16.0 14.8 15.5 16.4 14.2 14.7 C 14.4 15.1 C C C 15.2

4538 16.3 S 16.3 16.8 16.5 17.2 16.9 16.1 15.8 16.8 15.2 15.4 C C C C

4539 15.6 M M 15.9 17.5 16.6 12.0 16.8 16.1 14.6

4540 15.8 16.7 16.7 16. 4 M 15.1 15.7 15.9 16.1 15.2 C C 14.9 15.2

4541 15.6 C C 15.9 16.1 16.3 15.6 16.0 14.5 15.2 15.3 15.0 15.6

4542 15.5 16.1 1 5.7 14.4 16.4 15.4 14.6 15.6 15.2 16.3

4543 NOT PREG., NO MATING DATE

4544 NOT PREG ., NO MATING DATE

4545 14.4 15.0 15.1 C 15.4 14.5 14.9 14.3 14.6 12.6 14.6 12.6

4546 12.9 S D S 12.9 13.6 13.2 13.2 13.5 12.5 12.6 12.3 12.1 12.9 C

4547 14.0 C 15.8 15.3 14.0 C C C 13.5 14.2 C 13.6 13.4 13.2 13.3 13.8

4548 13.2 11.8 C 13.3 13.0 13.5 13.4 C 13.0 C C 12.8 13.2 13.1 15.0

4549 15.6 16.6 17.3 15.8 C 15.4 16.0 15.3 14.7 14.5 15.9 14.4 C

4550 13.4 C C C 13.6 13.9 13.5 12.2 13.3 C C 13.4 14.1 12.2 13.6 13.7

4551 NOT PREGNANT

MEAN 14.3

S.D. 1.09

N 22

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 394

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 24.2 23.7 24.9 23.7 26.5 23.1 22.8 25.0 C 24.4 24.4 23.1 C

15 27 26.4 C 26.3 C 25.8 26.1 26.2 27.3 27.2 27.9 24.2 26.5 26.8

1528 25.2 27.9 26.1 27.6 12.0 27.4 28.4 D D 26.8

1529 24.5 25.3 24.0 25.7 25.4 23.0 24.3 24.0 24.0 C 24.0 M 25.2

1530 34.2 35.0 33.5 34.2 34.8 33.3

1531 18.9 20.3 19.8 C 19.5 19.5 C C 18.6 19.5 18.7 16.8 17.3 18.7

1532 24.6 25.7 C C 25.7 C 25.5 26.8 24.8 25.0 22.9 23.5 23.2 23.4

1533 25.9 S 26.9 25.4 27.9 25.3 27.1 C 23.2 25.7 C 25.1 26.5 26.3 C C

1534 NOT PREGNANT

1535 19.8 19.0 D 18.9 18.7 21.7 D 18.9 18.7 21.0 19.5 22.0

1536 27.6 30.0 27.6 27.8 28.8 29.5 26.7 C 28.2 26.3 C 25.3 26.1

1537 28.7 C C C 28.5 30.2 28.9 27.9 28.9 C C 29.5 C 30.0 26.4 28.9 27.8

1538 NOT PREGNANT

1539 25.8 S C 25.7 25.6 28.0 25.2 27.4 26.2 24.5 25.2 C 25.4 24.9

1540 NOT PREG., NO MATING DATE

1541 27.0 28.4 28.1 26.8 C C 29.0 26.9 26.6 C C 24.9 26.4 27.6 25.4 M

1542 20.0 20.9 22.1 19.5 19.7 20.4 C 19.1 19.1 20.3 19 .5 19.5

1543 25.2 26.4 25.7 26.7 25.4 C 25.1 C 25.6 25.1 C C 25.8 24.2 C 21.8

1544 26.3 S C C 26.6 27.7 26.7 25.8 25.6 28.0 24.9 25.2 25.7 26.5

1545 22.1 C 21.6 23.8 21.7 22.6 22.0 C C 21.1 C 23.1 22.5 21.9 20.3 C

1546 26.5 27.3 25.8 26.4 27.8 27.3 26.3 26.2 C 26.1 25.9 C 25.8 C

1547 28.3 27.7 28.6 29.4 27.3 28.4 28.6

1548 NOT PREGNANT

1549 TOTAL LITTER LOSS

1550 23.1 C 23.6 M 22.9 24.5 22.3 23.5 23.7 23.8 23.3 22.3 20.9

1551 24.3 26.2 24.9 25.2 24.1 23.9 23.3 24.3 23.4 23.6

MEAN 25.2

S.D. 3.39

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 395

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 22.7 C C 22.4 C 22.4 C 22.1 C 24.9 23.5 24.3 C 22.2 20.6 22.4 21.9

2527 23.4 24.6 26.1 22.3 25.2 C 23.9 C C C 21.6 20.8 24.2 22.8 22.3

2528 22.2 23.2 23.1 22.7 23.0 23.5 C C 23.5 21.8 20.5 C C 19.9 21.0 C C

2529 22.0 C 21.5 C 23.2 22.5 23.1 23.3 C 20.8 19.8 21.6 22.6 21.8

2530 26.4 S 27.2 27.1 26.8 C 28.0 25.7 24.2 26.3 C C 26.3 26.0 26.1

2531 25.4 C 25.9 25.9 25 .4 25.5 C 26.6 C 23.8 C C 25.7 24.9 M 26.0 24.7

2532 23.6 22.6 23.6 23.8 22.2 C 24.0 C C 24.4 22.9 25.0 25.0 23.0

2533 27.4 28.0 C C C 28.6 26.6 29.2 C 27.7 C 27.9 26.9 25.1 27.3 26.7 C C

2534 23.9 22.2 C C 24.9 C C 25.7 23.2 25.6 24.4 21.7 23.0 24.8 23.1

2535 26.0 25.6 24.4 26.5 27.6 27.0 26.7 25.6 25.7 27.0 24.3

2536 HUMANE SACRIFICE

2537 29. 9 25.2 31.6 31.8 31.0

2538 26.3 25.2 25.2 28.2 D 27.8 26.7 23.7 M 26.3 26.5 26.7

25 39 24.5 24.0 25.7 24.9 25.2 25.0 24.1 24.3 C 24.8 C C 23.9 23.0 C

2540 24.2 23.4 24.8 C 25.1 24.9 23.9 24.8 24.3 23.4 23.9 23.5

2541 24.6 24.9 25.0 25.1 25.6 24.9 23.1 24.3 24.5 23.0 25.3 C C C C

2542 23.8 D 24.9 25.1 24.8 23.2 25.3 C 22.6 23.5 21.7 C 22.5 24.0

2543 27.3 C 28.3 29.1 28.1 C 25.3 28.3 27.6 24.5 C C 28.3 C 27.0 26.6 C

2544 NOT PREG., NO MATING DATE

2545 26.0 26.1 26.4 27.3 M 26.7 24.4 27.7 26.9 24.4 C 27.1 23.1

2546 27.9 28.1 29.0 26.4 C 28.7 C C 28.6 28.3 28.5 27.1 25.6 C 29.0

2547 25.4 26.1 24.7 26.6 25.5 25.3 25.4 C 24.5 24.9 25.1 C 25.6

2548 26.4 26.7 28.5 27.5 27.0 C 27.6 C C 26.6 25.4 25.6 22.2 26.5

2549 NOT PREG., NO MATING DATE

2550 22.4 S 14.9 26.4 17.1 22.2 C 24.7 23.2 22.4 26.3 25.2 21.8

2551 25.9 26.7 27.4 25.9 26.1 26.8 25.4 C C 25.2 25.3 24.5 25.6

MEAN 25.1

S.D. 2.01

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 396

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG /M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# ME AN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-------------------------------------------------------------------------------------------------------------- ---------------------

3526 NOT PREG., NO MATING DATE

3527 23.5 C C 23.3 25.0 24.6 C 23.6 C C 23.3 21.5 22.6 22.9 24.1 24.0 C

3528 26.2 26.9 24.1 27.0 25.2 28.7 C C C C C 22.5 25.5 27.4 27.6 27.3

3529 24.5 M C 23.3 26.7 24.6 26.3 26.0 C 24.3 C 23.2 24.9 23.5 22.5 C

3530 NOT PREG., NO MATING DATE

3531 22.5 C 25.0 24.8 23.6 20.0 C 22.8 C 20.9 23.9 19.0 23 .6 21.5

3532 24.9 C C 26.5 25.3 22.1 26.2 24.4 23.2 27.6 23.4 25.0 25.6

3533 21.5 22.8 C 21.7 C 21.4 22.8 21.0 21.8 21.3 C 20.7 21.0 20.3 C C

3534 TOTAL LITTER LOSS

3535 20.1 20.3 D 19.3 21.6 C 21.9 C 20.6 18.8 19.7 18.6 C C 21.1 18.7

3536 23.0 C 23.2 C C 24.9 23.1 23.6 23.3 22.9 22.0 21.7 23.8 21.8

3537 26.7 27.8 26.6 27.3 27.1 C 27.9 C 27.6 27.5 C 25.9 24.6 24.9

3538 25.6 25.2 C C 26.6 25.2 24.7 26.8 25.9 26.5 26.1 24.4 24.7

3539 25.9 25.8 25.4 22.5 23.3 28.5 C 28.2 26.1 C C 26.6 28.2 24.0

3540 25.0 25.2 26.0 27.8 26.4 25.4 C C 24.9 25.7 25.0 C 18.2 25.6

3541 29.3 31.2 28.9 32.1 28.0 31.4 C C C 21.6 C 28.7 31.0 30.6 29.3

3542 26.3 27.0 27.0 27.8 25.5 26.0 25.9 26.6 26.6 25.9 24.4

3543 23.1 24.1 25.8 21.8 23 .2 20.4 C C 23.5 23.6 24.2 C 22.4 C 22.1 C

3544 21.9 C 22.0 23.0 22.2 22.9 22.0 21.7 C 20.2 22.1 C 19.7 23.4

3545 27.4 28.6 27.5 28.4 27.7 28.2 C 25.2 28.3 25.1 28.2 C C 26.3

3546 25.1 C C 26.9 C 24.3 25.4 26.6 25.6 22.9 22.0 24.5 27.2 25.9 C

3547 21.9 23.8 22.0 21.8 22.6 20.7 22.6 21.7 21.1 22.0 20.3

3548 27.4 27.2 29.2 27.0 29.4 27.7 C 26.9 26.1 27.1 27.7 25.6

3549 25. 7 S 27.3 25.6 27.9 24.7 C 25.5 23.9 24.4 27.3 C C 25.6 24.7 C

3550 20.5 S C 21.4 21.0 C C 21.3 20.7 20.6 20.6 C C 20.7 C 19.0 20.7 19.1

35 51 25.3 24.7 26.2 28.6 26.3 25.0 26.4 23.1 C 25.6 C C C 23.1 23.7 C

MEAN 24.5

S.D. 2.37

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:56

Huntingdon Life Sciences 00

- 4205 PAGE 397

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

IN HALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 14

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 21.6 21.2 C 22.3 22.7 C 21.4 22.0 21.0 C 21.4 20.9 21.4 21.8

4527 26.6 S 26.9 27.2 26.9 C 26.5 27.4 25.0 26.0 26.2 25.9 28.0

4528 21.9 C 22.5 C C 21.9 23.2 22.4 C 21.6 22.3 21.6 C 21.8 19.2 22.4

4529 24.4 23.8 25.6 24.4 C 24.8 23.5 25.4 C C C C 24.4 23.0 24.7 24.8

4530 24.4 25.4 24.1 23.9 C 23.5 25.6 24.1 22.9 C C C 24.9 24.7 24.9 C

4531 23.6 24.1 23.6 23.5 24.9 C 24.6 C 23.1 22.6 22.1 23.0 24.0 C C

4532 23.6 C C 25.9 21.8 C 24.9 26.1 25.8 22.6 22.7 20.9 21.2 24.3 C

4533 22.3 S 22.2 23.7 22.6 21.6 19.7 23.1 23.2 22.6

4534 25.2 S S 25.1 25.2 24.1 25.3 25.3 25.5 27.3 26.1 21.7 26.7

4535 22.7 S 22.1 23.1 23.4 C 23.2 21.2 C C C C 22.0 22.5 22.9 23.9 22.5

4536 27.2 C 27.5 30.2 28.7 28.2 25.6 25.9 26.6 26.1 26.6 C 26.8 C C

4537 25.8 27.9 26.1 25.1 25.7 26.8 24.1 26.1 C 24.2 26.2 C C C 25.9

4538 28.3 S 28.2 29.4 28.4 28.9 29.5 27.2 28.9 29.4 26.2 26.6 C C C C

4539 24.6 M M 25.3 27.4 26.9 17.1 26.7 25.9 23.2

4540 27.5 28.9 29.4 29.1 M 25.5 27.0 28.2 29.2 25.9 C C 25.3 26.1

4541 25.3 C C 24.9 25.8 26.1 26.1 25.8 24.0 24.9 24.8 24.9 26.0

4542 25.0 24.9 26.3 24.2 25.8 25.0 23.6 25.4 25.0 25.1

4543 NOT PREG., NO MATING DATE

4544 NOT PREG., NO MATING DATE

4545 23.0 24.2 23.9 C 23.1 23.7 23.9 22.8 23.5 20.6 23.7 20.7

4546 22.5 S D S 22.1 23.7 22.4 23.2 23.3 21.8 22.1 22.0 21.5 22.8 C

4547 26.4 C 28.5 27.7 25.8 C C C 26.5 27.1 C 25.4 25.9 25.6 25.0 26.5

4548 21.6 17.8 C 21.9 22.2 22.0 22.3 C 21.7 C C 20.7 21.4 21.4 24.3

4549 26.1 26.2 29.1 26.7 C 25.4 26.0 26.2 25 .2 24.5 25.6 25.8 C

4550 23.7 C C C 24.7 24.4 24.2 23.3 23.4 C C 23.7 24.6 22.0 23.5 23.5

4551 NOT PREGNANT

MEAN 24.3

S.D. 1.82

N 22

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Sciences 00

- 4205 PAGE 398

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 41.6 42.3 43.7 40.9 45.4 39.2 39.6 43.6 C 42.1 41.2 37.6 C

1527 44.7 C 45.6 C 43.5 44.8 43.9 47.4 44.9 45.9 40.1 44.7 46.1

1528 44.5 50.5 45.9 49.7 48.3 18.6 50.8 D D 4 7.5

1529 46.2 45.7 45.4 49.5 47.4 43.7 47.1 41.3 47.3 C 45.4 M 49.3

1530 54.2 56.7 52.5 55.0 55.3 51.5

1531 31.8 31.7 32.4 C 32.3 32.9 C C 32.2 32.4 31.9 28.4 30.7 32.8

1532 38.4 39.4 C C 38.0 C 38. 7 41.5 40.6 39.7 35.9 35.9 37.5 37.2

1533 44.5 S 47.3 45.8 45.4 43.1 45.9 C 40.3 44.8 C 43.7 44.8 44.3 C C

1534 NOT PREGNANT

1535 32.6 30.2 D 29.6 29.5 37.6 D 27.4 32.0 33.8 34.1 39.0

1536 45.6 52.1 45.1 47.5 47.6 49.3 41.7 C 47.5 41.6 C 41.6 41.5

1537 47.6 C C C 45.6 49.3 45.0 48.1 48.8 C C 49.3 C 50.8 44.8 46.3 47.7

1538 NOT PREGNANT

1539 43.6 S C 44.3 42.8 48.5 43.6 45.8 42.8 40.2 40.7 C 43.8 43.2

1540 NOT PREG., NO MATING DATE

1541 43.7 46.6 46.0 43.8 C C 45.5 44.1 42.9 C C 38.8 41.8 44.6 42.9 M

1542 32.6 33.7 36.1 32.2 33.0 35.5 C 31.0 30.3 31.7 31.9 30.7

1543 45.7 48.0 47.5 50.7 46.3 C 44.1 C 44.5 45.2 C C 44.7 45.9 C 40.2

1544 44.5 S C C 45.2 48.5 46.5 44.9 42.5 46.2 40.7 42.7 42.7 45.2

1545 35.0 C 29.2 39.9 34.4 35.6 35.2 C C 34.2 C 39.3 35.4 35.3 32.0 C

1546 43.7 44.5 44.0 42.5 44.9 44.6 42.5 44.7 C 44.8 42.9 C 42.0 C

1547 46.5 49.6 48.3 47.1 42.0 46.0 46.1

1548 NOT PREGNANT

1549 TOTAL LITTER LOSS

1550 34.5 C 32.1 M 35.0 35.4 34.3 34.5 38.7 37.3 34.9 32.0 31.0

1551 38.1 40.7 41.2 40.2 39.2 39.5 35.0 35.4 35.7 35.6

MEAN 41.9

S.D. 5.89

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Scien ces 00

- 4205 PAGE 399

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 35.5 C C 36.2 C 33.3 C 37.0 C 34.9 37.2 40.6 C 33.2 33.3 35.3 33.6

2527 37.2 37.7 41.5 33.7 39.8 C 40.2 C C C 33.6 34.2 37.4 37.1 36.7

2528 37.8 39.1 37.9 40.9 36.2 39.1 C C 42.0 37.6 35.3 C C 34.2 35.5 C C

2529 38.4 C 36.2 C 39.5 40.1 39.9 41.8 C 37.5 32.5 38.5 37.9 39.6

2530 45.7 S 47.8 46.4 45.1 C 51.7 44.7 41.5 44.4 C C 46.2 44.1 45.3

2531 42.6 C 43.5 41.8 42.1 41.7 C 43.9 C 41.6 C C 42.1 44.2 M 43.8 41.1

2532 41.1 40.1 41.7 38.9 38.8 C 43.0 C C 41.5 40.2 43.8 42.2 41.2

2533 46.5 48.5 C C C 46.5 42.5 51.3 C 45.0 C 47.9 49.0 44.9 47.8 41.8 C C

2534 39.7 35.8 C C 41.7 C C 40.7 35.9 44.7 39.5 38.1 40.1 4 0.6 40.3

2535 41.7 41.4 36.7 41.7 44.5 43.7 42.5 39.2 44.1 43.3 40.0

2536 HUMANE SACRIFICE

2537 52.5 43.2 53.7 60.0 52.9

2538 43.9 42.6 41.5 48.0 D 47.8 43.3 38.8 M 44.1 43. 5 45.3

2539 41.6 42.1 42.3 43.2 44.9 40.6 40.9 40.9 C 43.5 C C 39.6 38.4 C

2540 40.9 39.3 39.6 C 44.1 39.1 42.3 42.6 41.4 4 0.2 40.1 39.9

2541 42.5 43.1 40.9 44.4 44.0 43.0 39.2 42.1 42.3 40.4 45.9 C C C C

2542 41.2 D 42.0 42.2 42.4 40.4 42.5 C 39.3 43.7 39.2 C 40.0 40.7

2543 48.5 C 49.6 53.8 49.8 C 43.9 51.7 48.6 44.0 C C 47.9 C 48.1 47.3 C

2544 NOT PREG., NO MATING DATE

2545 41.0 37.8 46.6 46.1 M 40.4 35.3 46.3 41.0 42.3 C 39.1 34.7

2546 49.3 50.7 49.5 48.7 C 5 3.2 C C 48.8 51.6 50.4 45.3 45.4 C 49.7

2547 38.7 39.0 38.6 42.0 39.3 39.7 38.3 C 36.8 39.4 35.9 C 38.1

2548 43.2 45.5 45.3 45.3 46.4 C 46.8 C C 45.0 40.6 41.3 35.6 39.8

2549 NOT PREG., NO MATING DATE

2550 37.8 S 22. 4 47.9 24.7 41.5 C 41.1 37.8 39.1 43.7 43.1 36.7

2551 45.2 45.9 47.7 46.3 45.6 46.0 42.8 C C 45.5 44.5 42.5 45.1

MEAN 42.3

S. D. 4.20

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Sciences 00

- 4205 PAGE 400

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 21

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREG., NO MATING DATE

3527 40.1 C C 40.9 41.9 41.1 C 38.5 C C 40.0 37.6 38.1 39.6 42.2 40.6 C

3528 44.4 44.1 39.7 47.5 44.5 47.3 C C C C C 38.6 42.1 47.3 48.5 44.7

3529 41.7 M C 39.7 44.8 41.9 45.6 44.4 C 41.8 C 39.0 42.3 39.1 38.7 C

3530 NOT PREG., NO MATING DATE

3531 36.7 C 44.1 38.7 43.3 28.9 C 34.7 C 33.1 40.3 27.2 40.8 35.7

3532 42.5 C C 46.6 42.6 35.5 42.8 41.0 40.8 48.8 39.8 41.7 45.6

3533 33.2 34.9 C 32.6 C 33.0 35.0 34.6 32.4 32.1 C 32.6 33.7 30.6 C C

3534 TOTAL LITTER LOSS

353 5 32.0 31.2 D 31.5 32.9 C 32.6 C 34.2 31.7 32.3 31.1 C C 33.2 28.8

3536 40.7 C 40.5 C C 44.1 40.5 42.0 42.9 40.6 38.5 38.5 40.7 38.5

3537 46.3 46.7 50.3 47.3 44.8 C 46.1 C 48.8 47.8 C 46.7 41.5 43.3

3538 41.9 38.3 C C 44.2 42.6 39.1 43.7 42.1 M 43.3 43.8 40.1

3539 37.5 36.4 37.3 31.7 34.9 43.8 C 39.2 37.2 C C 37.3 44.1 32.8

3540 44.9 44.4 45.7 50.4 44.8 44.2 C C 42.0 45.2 43.9 C D 43.5

3541 51.0 54.4 49.7 56.7 50.5 51.9 C C C 38.9 C 50.0 53.4 52.7 52.0

3542 46.1 48.1 45.6 51.3 45.7 43.6 42.5 48.7 46.3 46.7 42.2

3543 38.8 41.3 44.0 35.7 38.0 30.1 C C 40.8 38.8 44.5 C 35.7 C 38.9 C

3544 34.5 C 32.6 36.5 33.9 37.0 33.9 37.2 C 31.1 36.0 C 31.0 35.9

3545 45.5 49.9 46.3 47.9 48.1 44.8 C 43.1 46.8 40.1 44.6 C C 43.4

3546 44.0 C C 47.2 C 43.7 45.8 46.8 46.0 42.3 40.0 40.5 43.3 4 4.1 C

3547 35.8 38.1 39.1 33.6 32.5 35.5 38.5 35.7 34.7 36.5 33.8

3548 46.8 46.2 50.8 45.8 50.9 49.1 C 43.9 45.4 45.6 46.9 43.1

3549 41.8 S 43.0 39.5 45.4 41.3 C 39.5 39.8 40.5 47.2 C C 40.2 41.1 C

3550 31.5 S C 32.7 32.7 C C 31.8 31.6 31.5 31.5 C C 31.9 C 28.9 32.4 30.4

3551 43.4 42.6 45.7 47.0 44.8 43.2 45.9 40.5 C 44.0 C C C 40.2 40.0 C

MEAN 40.9

S.D. 5.19

N 23

PUP STATUS CODES: S- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Sciences 00

- 4205 PAGE 401

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERA TION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 21

----------------------------------------------------------------------------------------------------------------------------- ----- -

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 35.1 33.5 C 35.9 39.3 C 34.3 33.7 35.5 C 34.4 33.9 35.8 34.6

4527 44.9 S 44.4 45.9 44.7 C 45.3 48.2 41.7 45.4 44.3 42.0 47. 5

4528 38.3 C 40.6 C C 39.0 40.2 39.7 C 37.4 38.5 38.2 C 39.2 32.3 38.3

4529 40.2 42.0 42.0 39.2 C 38.8 42.4 41.1 C C C C 38.5 38.3 38.0 41.4

4530 37.8 40.0 38.2 36.1 C 35.6 37.5 37.7 35.0 C C C 38.1 39.5 40.7 C

4531 37.3 37.8 37.4 36.6 40.6 C 36.0 C 39.2 37.3 37.0 35.6 35.2 C C

4532 43.3 C C 46.6 42.2 C 46.4 46.0 46.9 40.9 40.9 38.2 38.4 46.0 C

4533 37.1 S 37.2 36.2 38.1 35.1 32.7 39.1 39.1 39.1

4534 43.8 S S 43.0 46.4 42.1 42.2 45.3 43.4 47.8 44.9 37.7 45.6

4535 39.3 S 40.0 40.6 42.9 C 38.6 3 6.3 C C C C 38.1 38.9 37.6 41.2 39.0

4536 48.8 C 50.4 51.3 51.2 51.2 48.3 46.9 46.1 44.3 49.6 C 48.4 C C

4537 44.0 48.2 40.6 42.3 45.9 45.9 38.7 44.7 C 42.5 44.2 C C C 47.4

4538 HUMANE SACRIFICE

4539 42.8 M M 43.9 50. 3 46.1 24.6 46.0 46.8 42.0

4540 46.1 50.2 49.8 47.3 M 45.0 46.7 45.9 47.9 43.3 C C 41.4 43.7

4541 44.0 C C 4 0.4 45.6 45.2 45.1 44.7 42.3 43.7 44.4 44.8 44.3

4542 46.6 46.7 48.6 46.3 48.3 47.3 44.4 47.2 41.5 48.9

4543 NOT PREG., NO MATING DATE

4544 NOT PREG., NO MATING DATE

4545 37.6 38.2 40.9 C 37.7 37.6 39.1 35.5 41.6 33.1 38.0 34.4

4546 39.2 S D S 37.6 40.8 40.0 41.5 42.6 38.0 38.4 37.7 36.6 38.5 C

454 7 44.8 C 49.9 47.0 45.8 C C C 46.7 43.7 C 44.6 39.0 43.7 44.8 43.3

4548 34.0 27.2 C 34.3 33.6 35.9 35.9 C 32.5 C C 33.4 34.5 33.8 38.8

4549 42.6 42.7 41.5 45.7 C 43.7 43.5 44.7 42.1 41.1 40.5 40.6 C

4550 39.7 C C C 41.5 42.9 39.2 38.2 39.4 C C 39.1 41.9 36.6 40.3 38.2

4551 NOT PREGNANT

MEAN 41.2

S.D. 3.99

N 22

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Sciences 00

- 4205 PAGE 402

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP I 0 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

1526 76.7 80.7 83.9 74.0 87.3 72.2 72.1 78.0 C 75.4 75.1 68.7 C

1527 82.6 C 84.7 C 83.9 83.0 82.1 88.1 86.4 83.4 72.7 80.1 81.6

1528 81.9 94.9 85.2 93.4 35.4 91.6 85.6 D D 87.4

1529 82.1 81.8 87.0 92.3 84.0 76.1 82.4 73.2 82.9 C 79.1 M 82.1

1530 98.7 105.7 105.0 94.2 98.7 89.9

1531 62.2 64.0 62.7 C 66.1 65.0 C C 64.0 61.5 59.1 57.3 61.5 60.9

1532 77.4 79.7 C C 78.8 C 78.8 84.2 83.8 80.7 71.2 71.4 70.3 75.1

1533 82.5 S 87.0 83.8 86.6 82.4 86.4 C 74.3 83.2 C 81.8 80.1 79.5 C C

1534 NOT PREGNANT

1535 65.1 59.7 D 61.8 60.9 74.4 D 55.4 64.7 66.2 66.9 75.9

1536 80.8 90.6 79.5 85.5 83.3 89.5 76.3 C 82.7 73.0 C 76.5 71.4

1537 84.1 C C C 83.0 88.6 82.2 90.0 89.5 C C 83.7 C 84.9 78.5 79.2 80.9

1538 NOT PREGNANT

1539 79.4 S C 85.0 81.8 88.2 81.2 88.1 78.3 69.3 69.9 C 75.9 76.7

1540 NOT PREG., NO MATING DATE

1541 82.9 89.9 90.5 85.6 C C 86.4 87.6 79.8 C C 6 6.9 79.4 83.1 80.0 M

1542 69.6 67.2 79.1 68.6 74.5 76.4 C 66.0 69.0 63.6 66.5 64.8

1543 80.3 82.4 87.3 88.8 82.3 C 78.6 C 77.1 79.9 C C 76.8 78.3 C 71.7

1544 82.5 S C C 84.8 95.0 87.7 87.9 82.1 80.2 73.8 79.5 74.4 79.6

1545 70.9 C 66.7 79.7 69.9 72.7 75.3 C C 67.9 C 70.9 70.5 71.2 64.4 C

1546 81.0 84.6 74.9 83.6 82.9 86.2 75.9 82.6 C 81.2 80.5 C 77.8 C

1547 84.7 89.1 88.8 89.1 79.1 82.6 79.5

1548 NOT PREGNANT

1549 TOTAL LITTER LOSS

1550 71.3 C 70.4 M 72.0 73.3 70.4 73.4 76.3 75.4 72.1 67.0 62.2

1551 73.2 80.1 80.7 77.6 73. 5 72.0 65.6 67.5 70.9 71.0

MEAN 78.6

S.D. 7.92

N 21

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Sciences 00

- 4205 PAGE 403

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY ST UDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP II 2000 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

2526 73.1 C C 74.9 C 74.6 C 76.8 C 73.7 77.4 78.3 C 68.3 65.5 72.1 69.3

2527 71.5 76.3 80.6 67.8 76.1 C 79.7 C C C 64.4 64.4 67.9 67.7 69.8

2528 71.9 76.2 74.7 74.2 74.5 75.0 C C 75.5 70.4 68.7 C C 63.2 66.2 C C

2529 70.9 C 69.1 C 73.3 75.0 76.2 75.9 C 60.5 68.7 69.4 69.5 71.8

2530 83.7 S 88.1 88.5 85.3 C 95.6 82.0 75.6 81.7 C C 82.6 78.5 79.1

2531 78.5 C 79.6 78.0 79.9 83.5 C 81.8 C 74.5 C C 76.1 79.5 M 77.9 74.0

2532 77.7 79.2 77.9 77.6 75.1 C 82.7 C C 79.7 72.4 80.0 77.6 75.2

2533 88.3 91.2 C C C 90.0 82.6 97.0 C 86.6 C 92.3 89.1 83.2 88.1 82.8 C C

2534 74.2 71.6 C C 75.8 C C 76.9 73.1 82.4 69.9 72.2 73.1 76.1 70.8

2535 80.1 83.7 69.8 81.3 84.8 83.9 82.6 78.1 81.5 80.6 74.7

2536 HUMANE SAC RIFICE

2537 91.4 77.3 93.8 105.5 88.9

2538 82.8 81.2 84.5 92.6 D 89.7 87.2 70.6 M 77.9 77.4 84.1

2539 76.7 80.8 76.4 81.5 81.1 82.6 71.1 71.7 C 80.0 C C 69.6 72.5 C

2540 77.0 74.3 74.1 C 85.1 76.9 80.4 80.2 78.7 73.8 75.4 71.5

2541 77.6 79.5 80.5 81.2 80.8 77.0 69.0 77.3 75.8 73.7 81.6 C C C C

2542 79.1 D 83.8 80.9 82.9 77.2 84.6 C 77.3 80.0 73.8 C 75.0 75.5

2543 86.9 C 93.2 97.1 92.9 C 82.1 93.4 83.0 78.4 C C 83.1 C 84.0 81.8 C

2544 NOT PREG., NO MATING DATE

2545 80.0 73.1 91.1 90.8 M 80.5 67.9 84.4 80.2 81.3 C 78.1 72.8

2546 90.2 96.5 90.8 91.0 C 99.0 C C 90.2 91.8 90.7 78.7 83.5 C 90.1

2547 68.3 71.2 72.0 73.4 71.6 69.7 62.3 C 66.9 64.6 65.6 C 66.0

2548 79.7 84.4 84.2 85.0 84.6 C 84.2 C C 79.0 75.7 80.2 65.3 74.3

2549 NOT PREG., NO MATING DATE

2550 68.9 S 42.3 89.6 46.6 70.9 C 74.0 70.6 69.1 75.6 81.1 68.9

2551 84.5 86.0 90.2 90.5 87.3 82.2 79.8 C C 81.5 84.5 80.6 82.7

MEAN 78.8

S.D. 6.60

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Hunti ngdon Life Sciences 00

- 4205 PAGE 404

A PPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP III 10000 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

3526 NOT PREG., NO MATING DATE

3527 73.0 C C 74.4 78.5 74.8 C 73.9 C C 73.0 69.8 69.5 69.4 76.8 69.4 C

3528 83.3 83.7 77.2 88.2 88.3 88.5 C C C C C 76.9 77.2 84.4 85.8 82.7

3529 78.8 M C 79.2 86.0 80.8 88.8 85.7 C 77.3 C 70.8 75.8 71.5 72.2 C

3530 NOT PREG., NO MATING DATE

3531 65.3 C 81.9 78.2 80.3 41.9 C 61.5 C 65.6 73.2 34.6 73.0 62.3

3532 76.9 C C 80.7 82.7 67.1 79.6 73.6 75.3 83.8 70.4 74.6 81.3

3533 62.6 68.1 C 65.7 C 61.7 68.9 62.5 59.5 57.2 C 61.5 60.9 60.3 C C

3534 TOTAL LITTER LOSS

3535 59.5 60.4 D 59.0 62.0 C 60.8 C 64.3 59.4 56.6 57.7 C C 58.8 55.9

3536 73.5 C 77.3 C C 80.1 73.6 76.5 76.9 7 3.2 70.5 68.1 71.6 67.2

3537 83.4 86.0 89.8 86.3 78.2 C 83.6 C 88.7 89.0 C 80.5 76.0 75.8

3538 77.8 77.5 C C 83.3 78.4 73.1 84.6 73.5 M 77.3 77.5 75.1

3539 72.1 72.8 75.5 61.3 71.5 85.2 C 74.9 69.0 C C 67.4 77.8 65.4

3540 83.7 85.1 85.6 93.0 87.8 85.3 C C 77.0 79.6 79.5 C D 80.2

3541 91.4 100.7 93.2 98.1 95.0 98.4 C C C 68.8 C 85.1 91.5 93.9 89.8

3542 83.6 87.2 87.0 93.7 82.9 8 1.7 80.9 85.3 80.4 83.6 73.7

3543 74.9 80.0 83.4 71.8 76.1 64.2 C C 75.3 71.7 81.5 C 71.6 C 73.2 C

3544 68.6 C 70.2 74.3 68.6 73.3 68.8 70.5 C 64.6 68.5 C 61.0 65.9

3545 84.5 92.3 85.6 91.3 90.1 85.7 C 82.1 83.2 74.3 81.6 C C 78.4

3546 81.1 C C 87.7 C 81.6 89.9 86.7 86.8 76.7 73.9 71.0 77.5 79.6 C

3547 70.3 76.1 75.3 69.0 66.9 73.0 78.4 67.5 63.7 70.0 63.4

3548 81.9 8 2.3 92.3 81.6 91.9 86.1 C 77.6 76.0 77.2 83.5 70.1

3549 78.7 S 80.8 78.3 86.9 78.4 C 77.7 73.9 74.7 84.0 C C 74.0 78.3 C

3550 64.8 S C 69.0 67.0 C C 63.8 69.6 65.2 60.4 C C 69.6 C 60.6 60.6 62.6

3551 83.9 82.7 85.5 89.3 89.1 88.6 88.1 79.3 C 82.4 C C C 78.1 76.1 C

ME AN 76.2

S.D. 8.22

N 23

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:53

Huntingdon Life Sciences 00

- 4205 PAGE 405

APPENDIX U F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP BODY WEIGHTS (GRAMS)

GROUP IV 20000 MG/M3 LACTATION DAY 28

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# MEAN PUP#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

-----------------------------------------------------------------------------------------------------------------------------------

4526 70.2 69.2 C 73.4 77.7 C 69.4 68.5 70.6 C 66.3 70.0 70.7 65.9

4527 81.0 S 82.3 80.0 81.0 C 82.0 91.0 73.1 84.1 72.9 77.7 85.4

4528 69.6 C 72.6 C C 73.3 74.4 73.9 C 69.5 68.3 68.6 C 68.7 58.5 68.1

4529 78.7 84.2 86.0 77.1 C 76.2 83.4 81.6 C C C C 73.4 74.5 72.8 77.3

4530 73.7 79.2 73.4 73.3 C 71.6 76.6 73.0 69.1 C C C 70.6 73.2 76.9 C

453 1 72.1 72.2 75.1 71.9 77.8 C 74.0 C 71.4 71.3 69.8 68.3 69.0 C C

4532 80.6 C C 85.5 80.5 C 83.9 88.0 92.5 74.7 74.7 74.1 69.6 82.5 C

4533 68.9 S 74.7 64.3 73.1 68.2 64.3 69.9 67.8 69.2

4534 80.4 S S 81.1 85.4 79.0 80.8 84.9 82.2 83.6 79.6 67.3 80.4

4535 72.5 S 71.8 70.7 80.6 C 74.0 70.0 C C C C 67.3 72.8 70.5 74.5 72.4

4536 86.9 C 91.5 94.1 89.4 95.0 89.5 86.1 79.5 73.5 86.1 C 84.4 C C

4537 82.2 93.1 70.6 80.6 87.0 87.0 71.4 79.9 C 83.9 81.7 C C C 87.0

4538 HUMANE SACRIFICE

4539 80.3 M M 84.0 95.6 83.9 50.9 85.6 84.2 77.8

4540 85.0 93.7 90.6 90.1 M 84.2 88.1 80.3 84.3 79.6 C C 77.2 82.2

4541 78.9 C C 70.7 81.4 87.9 84.4 84.1 69.4 77.3 77.2 76.7 79.4

4542 84.7 86.4 92.7 84.0 89.5 89.1 79.1 80.0 76.8 85.0

4543 NOT PREG., NO MATING DATE

4544 NOT PREG., NO MATING DATE

4545 71.5 72.4 78.8 C 73.5 75.7 75.5 70.4 74.5 61.5 69.3 63.6

4546 74.5 S D S 72.3 82.3 78.3 80.7 82.5 71.0 70.2 69.7 68.3 70.1 C

4547 81.9 C 88.1 89.8 85.7 C C C 84.9 87.5 C 81.5 69.6 76.7 78.3 76.9

4548 67.6 58.6 C 70.6 67.8 75.5 69.3 C 62.8 C C 64.0 65.7 66.9 74.8

4549 79.5 79.1 81.6 85.9 C 88.2 78.4 77.4 76.8 79.0 73.6 75.2 C

4550 71.2 C C C 75.3 80.7 72.8 68.5 69.9 C C 65.3 75.1 64.0 71.3 68.7

4551 NOT PREGNANT

MEAN 76.9

S.D. 5.85

N 22

P UP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED M

- MISSING11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 406

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1026 NO REMARKABLE OBSERVATIONS

1027 NO REMARKABLE OBSERVATIONS

1028 NO REMARKABLE OBSERVATIONS

1029 EXTERNAL EXAM ALOPECIA

- GENERAL

EXTERNAL FINDING NOT CONFIRMED

1030 NO REMARKABLE OBSERVATIONS

1031 LIVER DISCOLORED FOCI; SLIGHT

MEDIAN LOBE

- 0.1 CM IN DIAMETER, RED

1032 NO REMARKABLE OBSERVATIONS

1033 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, LESS THAN 0.1 CM IN DIAMETER, TAN

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

1034 NO REMARKABLE OBSERVATIONS

1035 NO REMARKABLE OBSERVATIONS

1036 LIVER DISCOLORED AREA(S); SLIGHT

MEDIAN LOBE

- 1.0 CM IN DIAMETER, BROWN

KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

1037 NO REMARKABLE OBSERVATIONS

1038 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 407

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1039 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

1040 NO REMARKABLE OBSERVATIONS

1041 NO REMARKABLE OBSERVATIONS

1042 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

EXTERNAL EXAM SWOLLEN DIGIT

FIRST DIGIT, LEFT FOREPAW

EXTERNAL FINDING CONFIRMED

TISSUE SAVED

1043 NO REMARKABLE OBSERVATIONS

1044 NO REMARKABLE OBSERVATIONS

1045 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

1046 NO REMARKABLE OBSERVATIONS

1047 NO REMARKABLE OBSERVATIONS

1048 NO REMARKABLE OBSERVATIONS

1049 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

1050 THYMUS DISCOLORED FOCUS/FOCI; MODERATE; BILATERAL

0.2 CM IN DIAMETER, RED

TISSUE SAVED

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 408

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1 051 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 409

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2026 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

2027 NO REMARKABLE OBSERVATIONS

2028 NO REMARKABLE OBSERVATIONS

2029 NO REMARKABLE OBSERVATIONS

2030 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

0.1 CM IN DIAMETER, RED

2031 LUNGS DISCOLORED FOCI; SLIGHT

RIGHT AND LEFT LOBES, RED, 0.2 CM IN DIAMETER

2032 NO REMARKABLE OBSERVATIONS

2033 GONADS DISCOLORED FOCI ON PROSTATE; SLIGHT

RIGHT DORSAL LOBE

- 0.1 CM IN DIAMETER, RED

2034 KIDNEY DILATED RENAL PELVIS; EXTREME; LEFT

2035 NO REMARKABLE OBSERVATIONS

2036 KIDNEY DILATED RENAL PELVIS; MODERATE; LEFT

2037 NO REMARKABLE OBSERVATIONS

2038 NO REMARKABLE OBSERVATIONS

2039 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 410

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCT ION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2040 NO REMARKABLE OBSERVATIONS

2041 NO REMARKABLE OBSERVATIONS

2042 NO REMARKABLE OBSERVATIONS

2043 NO REMARKABLE OBSERVATIONS

2044 THYMUS DISCOLORED FOCUS/FOCI; EXTREME; BILATERAL

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

2045 NO REMARKABLE OBSERVATIONS

2046 NO REMARKABLE OBSERVATIONS

2047 KIDNEY DILATED RENAL PELVIS; EXTREME; RIGHT

2048 NO REMARKABLE OBSERVATIONS

2049 THYMUS DISCOLORED FOCUS/FOCI; SLIGHT

RED, 0. 1

- 0.2 CM DIAMETER

TISSUE SAVED

2050 THYMUS DISCOLORED FOCUS/FOCI; MODERATE; BILATERAL

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

2051 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

0.1 CM IN DIAMETER, RED

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 411

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GEN ERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GRO UP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3026 NO REMARKABLE OBSERVATIONS

3027 NO REMARKABLE OBSERVATIONS

3028 NO REMARKABLE OBSERVATIONS

3029 THYMUS DISCOLORED FOCUS/FOCI; MODERATE; LEFT

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

3030 NO REMARKABLE OBSERVATIONS

3031 NO REMARKABLE OBSERVATIONS

3032 NO REMARKABLE OBSERVATIONS

3033 NO REMARKABLE OBSERVATIONS

3034 NO REMARKABLE OBSERVATIONS

3035 NO REMARKABLE OBSERVATIONS

3036 NO REMARKABLE OBSERVATIONS

3037 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

3038 KIDNEY DILATED RENAL PELVIS; MODERATE; BILATERAL

3039 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 412

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICI TY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3040 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- 0.1 CM IN DIAMETER, RED

LIVER ADHESION; EXTREME

MEDIAN AND RIGHT LOBES TO DIAPHRAGM

LIVER NODULE(S)

MEDIAN LOBE

- 0.5 CM IN DIAMETER, TAN, FIRM

NODULE SAVED WITH PART OF DIAPHRAGM

304 1 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, WHITE, 0.2 CM IN DIAMETER

3042 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

3043 HS GROSS EXAM FRACTURE(S)

NASAL BONES

TISSUE SAVED

KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

EXTERNAL EXAM ORAL SORE

HARD PALATE, WITH SCABS, 0.4 CM IN DIAMETER

TISSUE SAVED

EXTERNAL FINDING CONFIRMED

3044 DIED THYMUS DISCOLORED FOCUS/FOCI; MODERATE

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

SPLEEN ENLARGED; EXTREME

LIVER ENLARGED; EXTREME

ALL LOBES

3045 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

DIED=FOUND DEAD HS=HUMANE SACRIFICE11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 413

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3046 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

3047 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

3048 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

3049 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

30 50 KIDNEY CYSTIC KIDNEY; SLIGHT; LEFT

CORTEX, CLEAR 0.2 CM IN DIAMETER

3051 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN -2 010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 414

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4026 NO REMARKABLE OBSERVATIONS

4027 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, 0.1 CM IN DIAMETER

4028 LUNGS DISCOLORED FOCI; SLIGHT

DIAPHRAGMATIC LOBE

- 0.1 CM IN DIAMETER, RED

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

4029 NO REMARKABLE OBSERVATIONS

4030 NO REMARKABLE OBSERVATIONS

4031 NO REMARKABLE OBSERVATIONS

40 32 THYMUS DISCOLORED FOCUS/FOCI; SLIGHT; BILATERAL

RED, 0.1 CM IN DIAMETER

TISSUE SAVED

LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES

- TAN, 0.1 CM IN DIAMETER

4033 NO REMARKABLE OBSERVATIONS

4034 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

4035 LUNGS DISCOLO RED FOCI; MODERATE

ALL LOBES, WHITE, 0.1

- 0.5 CM IN DIAMETER

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

4036 GONADS DISCOLORED FOCI ON PROSTATE; MODER ATE

RIGHT VENTRAL LOBE

- 0.4 CM IN DIAMETER, RED

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 415

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPR ODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4037 NO REMARKABLE OBS ERVATIONS

4038 NO REMARKABLE OBSERVATIONS

4039 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

4040 NO REMARKABLE OBSERVATIONS

4041 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

4042 NO REMARKABLE OBSERVATIONS

4043 NO REMARKABLE OBSERVATIONS

4044 KIDNEY DISCOLORED FOCUS/FOCI; SLIGHT; RIGHT

CORTEX

- 0.2 CM IN DIAMETER, BROWN

4045 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

4046 NO REMARKABLE OBSERVATIONS

4047 NO REMARKABLE OBSERVATIONS

4048 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:58

Huntingdon Life Sciences 00

- 4205 PAGE 416

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4049 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

4050 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- 0.1 CM IN DIAMETER, TAN

4051 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 18:00

Huntingdon Life Sciences 00

- 4205 PAGE 417

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1526 LUNGS DISCOLORED FO CI; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE ESTRUS

1527 ESTROUS CYCLE DIESTRUS

1528 ESTROUS CYCLE ESTRUS

1529 ESTROUS CYCLE DIESTRUS

1530 ESTROUS CYCLE ESTRUS

1531 THORACIC CAVITY ENLARGED LYMPH NODE(S); MODERATE

SUBMANDULAR

TISSUE SAVED

EXTERNAL EXAM ALOPECIA

- GENERAL

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

1532 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

ESTROUS CYCLE DIESTRUS

1533 ESTROUS CYCLE ESTRUS

1534 NP EXTERNAL EXAM RED EXUDATE SNOUT

NEGATIVE

EXTERNAL EXAM MISSING DIGIT

EXTERNAL FINDING CONFIRMED

SALEWSKI TEST NO FOCI PRESENT

ESTROUS CYCLE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT11

- JAN

- 2010 18:00

Huntingdon Life Sciences 00

- 4205 PAGE 418

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1535 ESTROUS CYCLE DIESTRUS

1536 ESTROUS CYCLE DIESTRUS

1537 ESTROUS CYCLE DIESTRUS

1538 NP ESTROUS CYCLE DIESTRUS

SALEWSKI TEST NO FOCI PRESENT

1539 ESTROUS CYCLE DIESTRUS

1540 NNMD EXTERNAL EXAM SALEWSKI'S TE ST

NO FOCI PRESENT

ESTROUS CYCLE DIESTRUS

1541 EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

1542 ESTROUS CYCLE DIESTRUS

1543 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0. 1- 0.2 CM IN DIAMETER

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE ESTRUS

1544 ADRENALS DISCOLORED ADRENALS; SLIGHT; RIGHT

RED

ESTROUS CYCLE DIESTRUS

1545 ESTROUS CYCLE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE11

- JAN

- 2010 18:00

Huntingdon Life Sciences 00

- 4205 PAGE 419

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1546 ESTROUS CYCLE DIESTRUS

1547 ESTROUS CYCLE DIESTRUS

1548 NP EXTERNAL EXAM RED EXUDATE SNOUT

NEGATIVE

SALEWSKI TEST NO FOCI PRESENT

ESTROUS CYCLE ESTRUS

1549 TLL EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTE RNAL FINDING CONFIRMED

ESTROUS CYCLE ESTRUS

1550 ESTROUS CYCLE PROESTRUS

1551 LUNGS DISCOLORED FOCI; MODERATE

ALL LOB ES

- TAN, 0.1 CM IN DIAMETER

ESTROUS CYCLE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT TLL=TOTAL LITTER LOSS11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 420

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL P ARENTAL NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2526 EYES OPACITY; RIGHT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE PROESTRUS

2527 ESTROUS CYCLE ESTRUS

2528 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDINGS CONFIRMED

ESTROUS CYCLE DIESTRUS

2529 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- 0.1 CM IN DIAMETER, TAN

EXTERNAL EXAM ALOPECIA

- EXT REMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE METESTRUS

2530 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE ESTRUS

2531 ESTROUS CYCLE DIESTRUS

2532 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

2533 ESTROUS CYCLE DIESTRUS

2534 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, WHITE, 0.2 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 421

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHAL ATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2535 ESTROUS CYCLE ESTRUS

2536 HS GROSS EXAM FRACTURE(S)

NASAL BONE

- BILATERAL

SKULL SAVED

EXTERNAL EXAM INCISORS BROKEN/MISSING

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

2537 ESTROUS CYCLE DIESTRUS

2538 ESTROUS CYCLE DIESTRUS

2539 LUNGS DISCOLORED FOCI; SLIGHT

DIAPHRAGMATIC LOBE

- 0.1 C M IN DIAMETER, RED

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

2540 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES, 0.1 CM IN DIAMETER, RED

ESTROUS CYCLE ESTRUS

2541 ESTROUS CYCLE METESTRUS

2542 ESTROUS CYCLE ESTRUS

2543 PNMD ESTROUS CYCLE DIESTRUS

2544 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

PNMD=PREGNANT, NO MATING DATE NNMD=NOT PREG., NO MATING DATE HS=HUMANE SACRIFICE11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 422

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODU CTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2545 LUNGS DISCOLORED FOCI; SLI GHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

2546 ESTROUS CYCLE DIESTRUS

2547 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

ESTROUS CYCLE DIESTRUS

2548 EXTERNAL EXAM ALOPECIA

- GENERAL

EXTERNAL FINDING NOT CONFIR MED

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE ESTRUS

2549 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE DIESTRUS

2550 ESTROUS CYCLE DIESTRUS

2551 ESTROUS CYCLE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 423

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRO DUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3526 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE ESTRUS

3527 ESTROUS CYCLE ESTRUS

3528 ESTROUS CYCLE DIESTRUS

3529 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, T AN, 0.1

- 0.2 CM IN DIAMETER

ESTROUS CYCLE ESTRUS

3530 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE ESTRUS

3531 ESTROUS CYCLE DIESTRUS

3532 ESTROUS CYCLE DIESTRUS

3533 LUNGS DISCOLORED FOCI; SLIGHT; LEFT

LEFT LOBE, 0.1

- 0.2 CM IN DIAMETER, WHITE

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

3534 TLL LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

3535 ESTROUS CYCLE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NNMD=NOT PREG., NO MATING DATE TLL=TOTAL LITTER LOSS11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 424

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3536 ESTROUS CYCLE DIESTRUS

3537 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE PROESTRUS

3538 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES - T AN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

3539 ESTROUS CYCLE DIESTRUS

3540 ESTROUS CYCLE ESTRUS

3541 LUNGS DISCOLORED FOCI ; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

3542 THYMUS DISCOLORED FOCUS/FOCI; SLIGHT

BOTH LOBES, 0.1 - 0 .2 CM IN DIAMETER, RED

TISSUE SAVED

ESTROUS CYCLE DIESTRUS

3543 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

ESTROUS CYCLE DIESTRUS

3544 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE METESTRUS

3545 ESTROUS CYCLE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 425

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICI TY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3546 EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

3547 ESTROUS CYCLE DIESTRUS

3548 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- 0.1 CM IN DIAMETER, RED AND TAN

ESTROUS CYCLE DIESTRUS

3549 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, LESS THAN 0.1 CM IN DIAMETER , WHITE

ESTROUS CYCLE ESTRUS

3550 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

3551 ESTROUS CYCLE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 426

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION T OXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4526 ESTROUS CYCLE DIESTRUS

4527 ESTROUS CYCLE DIESTRUS

4528 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE ESTRUS

4529 EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONF IRMED

ESTROUS CYCLE PROESTRUS

4530 PNMD LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.2 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

4531 SPLEEN ADHESION; EXTREME

TO STOMACH WALL AND PANCREAS

TISSUE SAVED

ESTROUS CYCLE DIESTRUS

4532 LUNGS DISCOLORED FOCI; MODERATE

ALL LOBES

- TAN, 0.1 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

4533 ESTROUS CYCLE DIESTRUS

4534 ESTROUS CYCLE DIESTRUS

4535 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, 0.1 CM IN DIAMETER

EXTERNAL EXAM ALOPECIA

- GENERAL

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM ALOPECIA

- EXTREMITIES/SNOUT

EXTERNAL FINDING CONFIRMED

-----------------------------------------------------------------------------------------------------------------------------------

PNMD=PREGNANT, NO MATING DATE11

- JAN

- 2010 17:59

Huntingdon Life Sciences 00

- 4205 PAGE 427

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERAT ION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4535 (CONTINUED)

ESTROUS CYCLE DIESTRUS

4536 ESTROUS CYCLE DIESTRUS

4537 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- TAN, LESS THAN 0.1 CM IN DIAMETER

ESTROUS CYCLE METESTRUS

4538 HS GROSS EXAM HEMORRHAGE; MODERATE

SKULL

- LEFT FRONTAL/NASAL BONES, RED, 1 CM IN DIAMETER

SKULL SAVED

EXTERNAL EXAM RED EXUDATE SNOUT

EXTERNAL FINDING CONFIRMED

EXTERNAL EXAM SWOLLEN SNOUT

EXTERNAL FINDING CONFIRMED

TISSUE SAVED

EXTERNAL EXAM INCISORS MALOCCLUDED

EXTERNAL FINDING CONFIRMED

ESTROUS CYCLE DIESTRUS

4539 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, TAN, 0.2 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

4540 ESTROUS CYCLE DIESTRUS

4541 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, LESS THEN 0.1 CM IN DIAMETER, TAN

ESTROUS CYCLE ESTRUS

4542 ESTROUS CYCLE DIESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

HS=HUMANE SACRIFICE11

- JAN

- 2010 17:59

Huntingdon Life S ciences 00

- 4205 PAGE 428

APPENDIX V P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PARENTAL NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ANIMAL# ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4543 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE DIESTRUS

4544 NNMD EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE ESTRUS

4545 ESTROUS CYCLE ESTRUS

4546 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES

- LESS THAN 0.1 CM IN DIAMETER, TAN

ESTROUS CYCLE DIESTRUS

4547 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, 0.1

- 0.2 CM IN DIAMETER, WHITE

ESTROUS CYCLE DIESTRUS

4548 ESTROUS CYCLE ESTRUS

4549 ESTROUS CYCLE ESTRUS

4550 LUNGS DISCOLORED FOCI; SLIGHT

ALL LOBES, WHITE, 0.1

- 0.2 CM IN DIAMETER

ESTROUS CYCLE DIESTRUS

4551 NP EXTERNAL EXAM SALEWSKI'S TEST

NO FOCI PRESENT

ESTROUS CYCLE ESTRUS

-----------------------------------------------------------------------------------------------------------------------------------

NP=NOT PREGNANT NNMD=NOT PREG., NO MATING DATE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 429

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1526 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

6F K 28 ABDOMINAL CAVITY DIAPHRAGMATIC HERNIA

PART OF MEDIAN LOBE PROTRUDING THROUGH DIAPHRAGM

1.0 x 0.5 CM

TISSUE SAVED

8F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

1527 1M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

1528 2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

8F D 11 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

9F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 430

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATIO N

-----------------------------------------------------------------------------------------------------------------------------------

1529 1M K 28 GONADS UND ESCENDED TESTE(S); RIGHT

TISSUE SAVED

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMAR KABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

1530 1M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

2M K 28 NO R EMARKABLE OBSERVATIONS

3F K 28 NO REMARKABLE OBSERVATIONS

4F K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

1531 1M K 28 GONA DS UNDESCENDED TESTE(S); BILATERAL

TISSUES SAVED

2M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

1532 2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 431

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1532 (CONTINUED)

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

1533 1F S GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

1535 1M K 28 NO REMARKABLE OBSERVATIONS

2M D 8 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NE CROPSY

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F D 1 GROSS EXAM AUTOLYSIS

POSTMORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

1536 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLB ORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 432

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHO LE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 M G/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMAL E# PUP# STATUS DAY ORGAN OBSERVATION

----------------------------------------------------------------------------------------------------------------------------- ----- -

1536 (CONTINUED)

10F C 4 NO REMARKABLE OBSERVATIONS

1537 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIO NS

10M C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OB SERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMAR KABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

1539 1M S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

7M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

1541 2M K 28 NO REMARKABLE OBSERVATIONS

4 M C 4 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

7M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 433

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STU DY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1541 (CONTINUED)

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REM ARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

1542 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

1543 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

1544 1M S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 434

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1544 (CONTINUED)

10F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

1545 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

3M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

6M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUE SAVED

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

1546 1M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO RE MARKABLE OBSERVATIONS

1547 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 435

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOX ICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1549 TLL 1F S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

3M D 2 GROSS EXAM CANNIBALIZED

4M D 2 STOMACH MILK IN STOMACH

5M D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

6M D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

7M D 2 STOMACH NO MILK IN STOMACH

8M D 2 STOMACH NO MILK IN STOMACH

9F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

10F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

11F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

12F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

13F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

14F D 2 GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

1550 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 KIDNEY CYST(S); SLIGHT; RIGHT

CORTEX, CLEAR, 0.1 CM IN DIAMETER

TISSUE SAVED

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARK ABLE OBSERVATIONS

12F K 28 THYMUS SMALL; SLIGHT

TISSUE SAVED

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES : S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE

TLL=TOTAL LITTER LOSS22

- JA N- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 436

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

1551 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4F K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 437

APPEND IX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2526 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

11M K 28 NO REMARKABLE OBSERVATIONS

12M C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

2527 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSER VATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKAB LE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

2528 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

1 2F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 438

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2528 (CONTINUED)

15F C 4 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

2529 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3 M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

2530 1F S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

2531 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

4M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

6M C 4 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

H untingdon Life Sciences 00

- 4205 PAGE 439

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2531 (CONTINUED)

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

2532 1M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUE SAVED

3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

5M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

2533 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVAT IONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

17F C 4 NO REM ARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 440

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENS ATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2534 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSE RVATIONS

2535 2M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

2537 1M K 28 NO RE MARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4F K 28 NO REMARKABLE OBSERVATIONS

2538 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M D 0 GROSS EXAM CANNIBALIZED

LUNGS LUNG FLOTATION TEST

- FOUND DEAD

STOMACH MILK IN STOMACH

5M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

----------------------------------------------------------------------------------------------------------------------------- - -----

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 441

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2539 2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

5M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

6F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATI ONS

12F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE O BSERVATIONS

2540 1M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REM ARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

2541 1M K 2 8 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

TISSUE SAVED

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

1 2F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 442

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERV ATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2542 1M D 0 LUNGS LUNG FLOTATION TEST

- FOUND DEAD

STOMACH NO M ILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

5M K 28 NO REMARKAB LE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

2543 PNMD 1M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

2545 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE

PNMD=PREGNANT, NO MATING DATE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 443

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BO DY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG /M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2546 1M K 28 KIDNEY CYST(S); SLIGHT; LEFT

CORTEX

- CLEAR, 0.1 CM IN DIAMETER

TISSUE SAVED

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

2547 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

2548 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIO NS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OB SERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 444

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

2550 1F S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

2551 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKAB LE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Un determined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 445

APPENDIX W F1 GENERATION

GASOLINE DIPE VAP OR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3527 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIO NS

9M C 4 NO REMARKABLE OBSERVATIONS

10M K 28 NO REMARKABLE OB SERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMAR KABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

16F C 4 NO REMARKABLE OBSERVATIONS

3528 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

3529 2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon L ife Sciences 00

- 4205 PAGE 446

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3529 (CONTINUED)

15F C 4 NO REMARKABLE OBSERVATIONS

3531 1M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 EYES OPACITY; MODERATE; BILATERAL

TISSUE SAVED

EXTERNAL EXAM DOMED HEAD

EXTERNAL FINDING CONFIRMED

EXTREME INTERNAL HYDROCEPHALY, EXTREME DECREASE IN CORTICAL

THICKNESS, LATERAL VENTRICLES FILLED WITH DISCOLORED (RED)

FLUID, CEREBELLUM AND MEDULLA OBLANGATA MISSHAPEN

6M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 EXTERNAL EXAM DOMED HEAD

EXTERNAL FINDING CONFIRMED

EXTREME INTERNAL HYDROCEPHALY, EXTREME DECREASE IN CORTICAL

THICKNESS, LATERAL VENTRICALS FILLED WITH DISCOLORED (RED)

FLUID, CEREBELLUM MISSHAPEN

TISSUE SAVED

3532 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Hu ntingdon Life Sciences 00

- 4205 PAGE 447

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3533 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

3534 TLL 1F D 1 LUNGS LUNG FLOTATION TEST

- FOUND DEAD

STOMACH NO MILK IN STOMACH

KIDNEY ENLARGED KIDNEY(S); MODERATE; BILATERAL

2F D 1 GROSS EXAM CANNIBALIZED

GROSS EXAM SEX UNDETERMINED

GROSS EXAM AUTOLYSIS

POST

- MORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

3535 1M K 28 NO REMARKABLE OBSERVATIONS

2M D 1 GROSS EXAM AUTOLYSIS

POSTMORTEM CHANGES OB VIOUS AT THE TIME OF NECROPSY

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12M C 4 NO REMARKABLE OBSERV ATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABL E OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE

TLL=TOTAL LITTER LOSS22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 448

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR COND ENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-------------------------------------------------------------------------------------------------------------- ---------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3536 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

3537 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REM ARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

3538 1M K 2 8 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

3539 1M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 449

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY OR GAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3539 (CONTINUED)

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

3540 1M K 28 KIDNEY CYST(S); SLIGHT; RIGHT

CORTEX

- 0.1 CM IN DIAMETER, CLEAR

TISSUE SAVED

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 KIDNEY CYST(S); SLIGHT; BILATERAL

CORTEX

- 0.1 CM IN DIAMETER, CLEAR

TISSUES SAVED

9F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F D 16 HEAD V DOMED HEAD; MODERATE

EXTREME INTERNAL HYDROCEPHALY

EYES OPACITY; BILATERAL

13F K 28 NO REMARKABLE OBSERVATIONS

3541 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 KIDNEY CYST(S); EXTREME; LEFT

CORTEX, MULTIPLE, 0.2

- 2.0 CM IN DIAMETER, CLEAR

TISSUE SAVED

KIDNEY DILATED RENAL PELVIS; EXTREME; LEFT

KIDNEY DISCOLORED; EXTREME; LEFT

PALE

TISSUE SAVED

6M C 4 NO REMARKABLE OBSERVATION S

7M C 4 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBS ERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE22- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 450

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3541 (CONTINUED)

11F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

3542 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUE SAVED

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

3543 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REM ARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

3544 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 451

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3545 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F C 4 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

3546 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

4M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

3547 2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

3548 1M K 28 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 GONADS UNDESCENDED TESTE(S); RIGHT

TISSUE SAVED

6F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 452

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-------------------------------------------------------------------------------------------------------------------------------- ---

3548 (CONTINUED)

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

3549 1F S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSE RVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

3550 1F S GROSS EXAM AUTOLYSIS

POSTMORTEM CHANGES OBVIOUS AT THE TIME OF NECROPSY

2M C 4 NO REMARKABLE OBSERVAT IONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO REM ARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

17F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 453

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTIO N TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

3551 2M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKA BLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Fem ale, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 454

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4526 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATI ONS

6M K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMA RKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

4527 1F S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

4528 1M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SC HEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 455

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4529 3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11M C 4 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

4530 PNMD 1M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUE SAVED

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

4531 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMAR KABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U =Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE

PNMD=PREGNANT, NO MATING DATE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 456

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICIT Y STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4531 (CONTINUED)

14F C 4 NO REMARK ABLE OBSERVATIONS

4532 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

5M C 4 NO REMARKABLE OBSERVATIONS

7M K 28 NO RE MARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

4533 1M S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

STOMACH SMALL; EXTREME

KIDNEY M FUSED KIDNEY

INTESTINES A UNDERDEVELOPMENT OF INTESTINE

INTESTINES REDUCED TO A SMALL MASS.

INTESTINES ANAL ATRESIA

GONADS UNDESCENDED TESTE(S); RIGHT

ABDOMINAL CAVITY GASTROSCHISIS

FISSURE OF ABDOMINAL WALL AT THE MEDIAN LINE.

SMALL PART OF INTESTINES PROTUDE.

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male , F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 457

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4534 1M S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

2F S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN ST OMACH

4M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMA RKABLE OBSERVATIONS

9M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

4535 1M S LUNGS LUNG FLOTATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

4M K 28 NO REMARKABLE OBSERVATIONS

5M C 4 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M C 4 NO REMARKABLE OBSERVATIONS

9M C 4 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

11M C 4 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

16F K 28 NO REMARKABLE OBSERVATIONS

4536 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 458

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4536 (CONTINUED)

14F C 4 NO REMARKABLE OBSERVATIONS

4537 1M K 28 GONADS UNDESCENDED TESTE(S); BILATERAL

TISSUES SAVED

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F C 4 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

4538 HS 1U S GROSS EXAM CANNIBALIZED

GROSS EXAM SEX UNDETERMINED

2M U 14 NO REMARKABLE OBSERVATIONS

3M U 14 NO REMARKABLE OBSERVATIONS

4M U 14 NO REM ARKABLE OBSERVATIONS

5M U 14 NO REMARKABLE OBSERVATIONS

6M U 14 NO REMARKABLE OBSERVATIONS

7F U 14 NO REMARKABLE OBSERVATIONS

8F U 14 NO REMARKABLE OBSERVATIONS

9F U 14 NO REMARKABLE OBSERVATIONS

10F U 14 NO REMARKABLE OBSERVATIONS

11F U 14 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

13F C 4 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

15F C 4 NO REMARKABLE OBSERVATIONS

4539 3M K 28 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

5F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN C

- CULLED K

- SCHEDULED SACRIFICE U

- UNSCHEDULED SACRIFICE

HS=HUMANE SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 459

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4539 (CONTINUED)

6F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

4540 3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

4541 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

4M K 28 KIDNEY DILATED RENAL PELVIS; MODERATE; RIGHT

TISSUE SAVED

5M K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

4542 2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6F K 28 NO REMARKABLE OBSERVATIONS

7F K 28 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

4545 1M K 28 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIO NS

6M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 460

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4545 (CONTINUED)

8F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

4546 1F S LUNGS LUNG FLOTATION TEST

- STILL BORN

STOMACH NO MILK IN STOMACH

2F D 0 STOMACH NO MILK IN STOM ACH

LUNGS LUNG FLOTATION TEST

- FOUND DEAD

3F S LUNGS LUNG FLOT ATION TEST

- STILLBORN

STOMACH NO MILK IN STOMACH

4M K 28 N O REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

14F C 4 NO REMARKABLE OBSERVATIONS

4547 1M C 4 NO REMARKABLE OBSERVATIONS

2M K 28 NO REMARKABLE OBSERVATIONS

3M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

5M C 4 NO REMARKABLE OBSERVATIONS

6M C 4 NO REMARKABLE OBSERVATIONS

7M C 4 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

10M C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

15F K 28 NO REMARKABLE OBSERVATIONS

4548 1M K 28 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: S

- STILLBORN D

- DIED C

- CULLED K

- SCHEDULED SACRIFICE22

- JAN

- 2010 12:33

Huntingdon Life Sciences 00

- 4205 PAGE 461

APPENDIX W F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP NECROPSY OBSERVATIONS

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

FEMALE# PUP# STATUS DAY ORGAN OBSERVATION

-----------------------------------------------------------------------------------------------------------------------------------

4548 (CONTINUED)

6M K 28 NO REMARKABLE OBSERVATIONS

7F C 4 NO REMARKABLE OBSERVATIONS

8F K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

11F K 28 NO REMARKABLE OBSERVATIONS

12F K 28 THYMUS DISCOLORED FOCI; MODERATE

BOTH LOBES

- 0.1 CM IN DIAMETER, RED

TISSUE SAVED

4549 3M K 28 KIDNEY DILATED RENAL PELVIS; SLIGHT; RIGHT

TISSUE SAVED

4M C 4 NO REMARKABLE OBSERVATIONS

5M K 28 NO REMARKABLE OBSERVATIONS

6M K 28 GONADS UNDESCENDED TESTE(S); LEFT

TISSUE SAVED

7F K 28 NO REMARKABLE OBSERVATIONS

9F K 28 NO REMARKABLE OBSERVATIONS

10F K 28 NO REMARKABLE OBSERVATIONS

12F C 4 NO REMARKABLE OBSERVATIONS

4550 1M C 4 NO REMARKABLE OBSERVATIONS

2M C 4 NO REMARKABLE OBSERVATIONS

3M C 4 NO REMARKABLE OBSERVATIONS

4M K 28 NO REMARKABLE OBSERVATIONS

7M K 28 NO REMARKABLE OBSERVATIONS

8M K 28 NO REMARKABLE OBSERVATIONS

9F C 4 NO REMARKABLE OBSERVATIONS

10F C 4 NO REMARKABLE OBSERVATIONS

12F K 28 NO REMARKABLE OBSERVATIONS

13F K 28 NO REMARKABLE OBSERVATIONS

14F K 28 NO REMARKABLE OBSERVATIONS

-----------------------------------------------------------------------------------------------------------------------------------

SEX CODES: M

- Male, F=Female, U=Undetermined

PUP STATUS CODES: C

- CULLED K

- SCHEDULED SACRIFICE

# Huntingdon Life Sciences 00

- 4205 Page 462

211

- DIPE

- 1G Final Report

Individual Parental Organ Weights

Preface Appendix X

Key to Abbreviations:

g = Grams

FBW = Final Body Weight

SEM. VES & CO.GL. = Seminal Vesicles and Coagulating Glands

NOTES:

1. Due to an apparent weighing error, the right epididymis weight for Animal

No. 2043 is not presented in the following appendix.

2. Du e to computer limitation, values for %FBW less than 0.0001 do not

appear in this appendix.11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 463

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TESTIS

- RIGHT TESTIS

- LEFT TESTES PROSTATE

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1026 596 1.7993 0.0030 0.8163 1.7939 0.0030 0.8139 3.5711 0.0060 1.6201 1.0581 0.0018 0.4800

1027 595 1.7557 0.0030 0.7673 1.7465 0.0029 0.7633 3.4946 0.0059 1.5273 1.0416 0.0018 0.4552

102 8 554 1.6881 0.0030 0.7468 1.6812 0.0030 0.7437 3.3187 0.0060 1.4681 1.7335 0.0031 0.7669

1029 522 1.5302 0.0029 0.7426 1.5624 0.0030 0.7582 3.0878 0.0059 1.4985 0.7730 0.0015 0.3751

1030 574 1.7717 0.0031 0.7913 1.8094 0.0032 0.8081 3.5800 0.0062 1.5989 1.5824 0.0028 0.7067

1031 547 1.5122 0.0028 0.7255 1.4277 0.0026 0.6849 2.9311 0.0054 1.4062 1.0058 0.0018 0.4825

1032 621 1.8170 0.0029 0.7711 1.8152 0.0029 0.7703 3.6322 0.0058 1.5414 1.5616 0.0025 0.6627

1033 561 1.9301 0.0034 0.8254 1.9521 0.0035 0.8348 3.8788 0.0069 1.6588 0.6683 0.0012 0.2858

1034 512 1.4359 0.0028 0.7017 1.5098 0.0030 0.7378 2.9438 0.0058 1.4385 1.2219 0.0024 0.5971

1035 535 1.8372 0.0034 0.8900 1.8268 0.0034 0.8850 3.6543 0.0068 1.7703 1.4368 0.0027 0.6961

1036 597 1.8088 0.0030 0.8104 1.9348 0.0032 0.8668 3.7108 0.0062 1.6625 1.4726 0.0025 0.6598

1037 522 1.5800 0.0030 0.7539 1.5592 0.0030 0.7439 3.1302 0.0060 1.4935 1.1181 0.0021 0.5335

1038 582 1.5886 0.0027 0.7629 1.5947 0.0027 0.7658 3.1793 0.0055 1.5267 1.0151 0.0017 0.4875

1039 510 1.5938 0.0031 0.7586 1.5508 0.0030 0.7381 3.1318 0.0061 1.4906 1.1260 0.0022 0.5359

1040 661 1.8173 0.0027 0.8110 1.7817 0.0027 0.7952 3.5884 0.0054 1.6015 1.4564 0.0022 0.6500

1041 683 1.8513 0.0027 0.8461 1.8152 0.0027 0.8296 3.6553 0.0054 1.6705 1.0450 0.0015 0.4776

1042 687 1.7695 0.0026 0.7792 1.7796 0.0026 0.7837 3.5494 0.0052 1.5630 1.0055 0.0015 0.4428

1043 445 1.7503 0.0039 0.8071 1.6880 0.0038 0.7783 3.4333 0.0077 1.5831 1.0918 0.0025 0.5034

1044 635 1.5616 0.0025 0.7607 1.6105 0.0025 0.7845 3.1681 0.0050 1.5432 1.3382 0.0021 0.6519

1045 555 1.7361 0.0031 0.8638 1.7920 0.0032 0.8916 3.5117 0.0063 1.7472 1.2788 0.0023 0.6363

1046 594 1.6286 0.0027 0.7228 1.5760 0.0027 0.6994 3.2102 0.0054 1.4247 1.4828 0.0025 0.6581

1047 530 1.7351 0.0033 0.8288 1.7488 0.0033 0.8353 3.4905 0.0066 1.6673 1.1052 0.0021 0.5279

1048 625 1.4819 0.0024 0.6557 1.4681 0.0023 0.6496 2.9271 0.0047 1.2952 1.2858 0.0021 0.5689

1049 605 1.9999 0.0033 0.9230 2.0042 0.0033 0.9250 4.0212 0.0066 1.8558 1.3470 0.0022 0.6217

1050 597 1.7741 0.0030 0.7531 1.7452 0.0029 0.7408 3.5344 0.0059 1.5003 0.9414 0.0016 0.3996

1051 505 1.6026 0.0032 0.7953 1.5959 0.0032 0.7919 3.2030 0.0063 1.5894 1.2348 0.0024 0.6127

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 464

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODU CTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TESTIS

- RIGHT TESTIS

- LEFT TESTES PROSTATE

Animal FINAL BODY

------------------------- ------------------------- --- ---------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2026 505 1.5245 0.0030 0.7001 1.6044 0.0032 0.7367 3.1258 0.0062 1.4354 1.0736 0.0021 0.4930

2027 511 1.6583 0.0032 0.7244 1.7121 0.0033 0.7479 3.3680 0.0066 1.4713 1.0069 0.0020 0.4398

2028 567 1.7076 0.0030 0.7873 1.6956 0.0030 0.7817 3.3713 0.0060 1.5543 1.5233 0.0027 0.7023

2029 588 1.6714 0.0028 0.7867 1.6794 0.0029 0.7905 3.3367 0.0057 1.5706 1.1011 0.0019 0.5183

2030 625 1.6981 0.0027 0.7488 1.6291 0.0026 0.7184 3.3269 0.0053 1.4671 1.1333 0.0018 0.4998

2031 516 1.7142 0.0033 0.8084 1.6127 0.0031 0.7605 3.3238 0.0064 1.5675 1.3548 0.0026 0.6389

2032 492 1.8586 0.0038 0.8846 1.7882 0.0036 0.8511 3.6445 0.0074 1.7346 1.1312 0.0023 0.5384

2033 639 1.5580 0.0024 0.7042 1.6148 0.0025 0.7299 3.1729 0.0050 1.4341 1.0875 0.0017 0.4915

2034 608 1.7149 0.0028 0.8006 1.6876 0.0028 0.7879 3.4010 0.0056 1.5878 1.1032 0.0018 0.5151

2035 504 1.6905 0.0034 0.8342 1.6329 0.0032 0.8058 3.3173 0.0066 1.6370 0.9001 0.0018 0.4442

2036 491 1.7842 0.0036 0.7794 1.7415 0.0035 0.7607 3.4955 0.0071 1.5270 1.2491 0.0025 0.5456

2037 572 1.8589 0.0032 0.8302 1.9142 0.0033 0.8549 3.7319 0.0065 1.6668 1.2487 0.0022 0.5577

2038 540 1.6706 0.0031 0.7739 1.7581 0.0033 0.8144 3.4002 0.0063 1.5750 1.4708 0.0027 0.6813

2039 582 1.4696 0.0025 0.6710 1.6069 0.0028 0.7337 3.0614 0.0053 1.3978 1.1126 0.0019 0.5080

2040 581 1.6926 0.0029 0.8062 1.6572 0.0029 0.7894 3.3441 0.0058 1.5929 1.5279 0.0026 0.7278

2041 614 1.8165 0.0030 0.8434 1.8179 0.0030 0.8440 3.6254 0.0059 1.6832 0.9897 0.0016 0.4595

2042 551 1.5531 0.0028 0.7493 1.5118 0.0027 0.7294 3.0543 0.0055 1.4737 1.0920 0.0020 0.5269

2043 640 1.6935 0.0026 0.7678 1.6895 0.0026 0.7660 3.3691 0.0053 1.5275 1.2020 0.0019 0.5450

2044 674 1.6068 0.0024 0.7526 1.5856 0.0024 0.7427 3.1837 0.0047 1.4913 0.8473 0.0013 0.3969

2045 664 1.6855 0.0025 0.7908 1.6646 0.0025 0.7810 3.3423 0.0050 1.5681 1.1005 0.0017 0 .5163

2046 563 1.5739 0.0028 0.7481 1.5524 0.0028 0.7379 3.1279 0.0056 1.4867 1.3885 0.0025 0.6600

2047 598 1.6604 0.0028 0.7845 1.6592 0.0028 0.7840 3.3100 0.0055 1.5640 1.2626 0. 0021 0.5966

2048 495 1.7914 0.0036 0.8384 1.8012 0.0036 0.8430 3.5840 0.0072 1.6774 1.0083 0.0020 0.4719

2049 697 1.7536 0.0025 0.7800 1.7164 0.0025 0.7634 3.4671 0.0050 1.5421 1.1086 0.0016 0.4931

2050 606 1.5748 0.0026 0.7172 1.5947 0.0026 0.7262 3.1389 0.0052 1.4294 0.9434 0.0016 0.4296

2051 545 1.6132 0.0030 0.7408 1.6850 0.0031 0.7738 3.2996 0.0061 1.5153 0.7812 0.0014 0.3588

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 465

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TESTIS

- RIGHT TESTIS

- LEFT TESTES PROSTATE

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

---------------------------------------------------------------------------------------------------------------------------------- -

3026 575 1.6386 0.0028 0.7780 1.6814 0.0029 0.7983 3.3205 0.0058 1.5766 0.9738 0.0017 0.4624

3027 485 1.7334 0.0036 0.8623 1.7602 0.0036 0.8757 3.4877 0.0072 1.7351 0.9520 0.0020 0.4736

3028 625 1.8099 0.0029 0.8058 1.7912 0.0029 0.7975 3.5665 0.0057 1.5879 1.7572 0.0028 0.7823

3029 611 1.6481 0.0027 0.7617 1.5417 0.0025 0.7125 3.1848 0.0052 1.4719 1.3883 0.0023 0.6416

3030 627 1.9387 0.0031 0.8891 1.9716 0.0031 0.9042 3.8900 0.0062 1.7841 1.3376 0.0021 0.6135

3031 524 1.5786 0.0030 0.7600 1.5739 0.0030 0.7578 3.1412 0.0060 1.5124 1.1755 0.0022 0.5660

3032 510 1.5368 0.0030 0.7155 1.6427 0.0032 0.7648 3.1535 0.0062 1.4682 1.0800 0.0021 0.5028

3033 587 1.6921 0.0029 0.7995 1.6596 0.0028 0.7842 3.3457 0.0057 1.5808 1.6520 0.0028 0.7806

3034 594 1.7101 0.0029 0.7528 1.7133 0.0029 0.7542 3.3816 0.0057 1.4886 0.8583 0.0014 0.3778

3035 624 2.0352 0.0033 0.8802 2.0943 0.0034 0.9057 4.1095 0.0066 1.7772 1.5748 0.0025 0.6811

3036 505 1.7243 0.0034 0.8159 1.7844 0.0035 0.8443 3.4736 0.0069 1.6435 1.8031 0.0036 0.8531

3037 577 1.7958 0.0031 0.8231 1.7626 0.0031 0.8079 3.5381 0.0061 1.6217 1.2175 0.0021 0.5581

3038 569 1.7906 0.0031 0.8153 1.8088 0.0032 0.8236 3.5855 0.0063 1.6326 1.3860 0.0024 0.6311

3039 568 1.8495 0.0033 0.8419 1.8858 0.0033 0.8584 3.7134 0.0065 1.6904 0.9385 0.0017 0.4272

3040 660 1.6888 0.0026 0.7879 1.7724 0.0027 0.8269 3.4591 0.0052 1.6138 1.2118 0.0018 0.5653

3041 629 1.5568 0.0025 0.7240 1.5861 0.0025 0.7376 3.1365 0.0050 1.4586 1.3628 0.0022 0.6337

3042 553 1.7494 0.0032 0.8242 1.7364 0.0031 0.8181 3.4694 0.0063 1.6345 1.0542 0.0019 0.4967

3045 476 1.6271 0.0034 0.7336 1.6651 0.0035 0.7507 3.2985 0.0069 1.4872 1.0570 0.0022 0.4766

3046 536 1.8476 0.0034 0.8550 1.8307 0.0034 0.8472 3.6691 0.0068 1.6979 1.1521 0.0021 0.5331

3047 559 1.9826 0.0035 0.9524 2.0046 0.0036 0.9630 3.9827 0.0071 1.9133 1.3078 0.0023 0.6283

3048 559 1.6910 0.0030 0.8038 1.6290 0.0029 0.7743 3.3218 0.0059 1.5790 1.2461 0.0022 0.5923

3049 550 1.9390 0.0035 0.8704 1.9360 0.0035 0.8691 3.8820 0.0071 1.7427 1.0790 0.0020 0.4844

3050 660 1.6530 0.0025 0.6948 1.6370 0.0025 0.6881 3.2650 0.0049 1.3724 1.2808 0.0019 0.5384

3051 646 1.7778 0.0028 0.8149 1.7567 0.0027 0.8052 3.5284 0.0055 1.6173 1.3625 0.0021 0.6245

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 466

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

TESTIS

- RIGHT TESTIS

- LEFT TESTES PROSTATE

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4026 642 1.7615 0.0027 0.8302 1.7261 0.0027 0.8135 3.4808 0.0054 1.6404 1.3245 0.0021 0.6242

4027 512 1.5946 0.0031 0.7306 1.5652 0.0031 0.7172 3.1547 0.0062 1.4455 1.2075 0.0024 0.5533

4028 597 1.9704 0.0033 0.9385 1.8835 0.0032 0.8971 3.8503 0.0065 1.8339 0.8864 0.0015 0.4222

4029 657 1.8850 0.0029 0.8429 1.9263 0.0029 0.8613 3.7879 0.0058 1.6937 1.0052 0.0015 0.4495

4030 591 1.7362 0.0029 0.7963 1.8032 0.0031 0.8270 3.5260 0.0060 1.6172 1.2066 0.0020 0.5534

4031 504 1.7098 0.0034 0.7742 1.6729 0.0033 0.7574 3.3677 0.0067 1.5248 1.2415 0.0025 0.5621

4032 783 1.5324 0.0020 0.7186 1.4908 0.0019 0.6991 3.0010 0.0038 1.4073 1.1647 0.0015 0.5462

4033 530 1.8109 0.0034 0.8655 1.7504 0.0033 0.8366 3.5416 0.0067 1.6927 1.4101 0.0027 0.6739

4034 553 1.7124 0.0031 0.8533 1.6687 0.0030 0.8315 3.3608 0.0061 1.6747 1.1010 0.0020 0.5486

4035 498 1.7213 0.0035 0.8608 1.7647 0.0035 0.8825 3.4675 0.0070 1.7341 1.2432 0.0025 0.6217

4036 596 1.8719 0.0031 0.8477 1.8484 0.0031 0.8370 3.7217 0.0062 1.6853 0.8787 0.0015 0.3979

4037 550 1.7254 0.0031 0.8475 1.8409 0.0033 0.9043 3.5455 0.0065 1.7416 1.8010 0.0033 0.8847

4038 520 1.7512 0.0034 0.8566 1.7068 0.0033 0.8349 3.4405 0.0066 1.6830 1.7594 0.0034 0.8606

4039 552 2.0844 0.0038 1.0057 2.0478 0.0037 0.9881 4.1307 0.0075 1.9931 1.0967 0.0020 0.5292

4040 552 1.8674 0.0034 0.8782 1.8224 0.0033 0.8570 3.6792 0.0067 1.7302 1.4292 0.0026 0.6721

40 41 530 1.6517 0.0031 0.7851 1.6291 0.0031 0.7743 3.2755 0.0062 1.5569 0.7926 0.0015 0.3767

4042 595 1.7660 0.0030 0.8193 1.6963 0.0028 0.7870 3.4475 0.0058 1.5995 0.9881 0.0017 0.4584

4043 521 1.6245 0.0031 0.7721 1.6510 0.0032 0.7847 3.2654 0.0063 1.5521 0.8865 0.0017 0.4214

4044 541 1.7284 0.0032 0.8179 1.8509 0.0034 0.8759 3.5761 0.0066 1.6923 1.0462 0.0019 0.4951

4045 566 1.8256 0.0032 0.8568 1.7698 0.0031 0.8307 3.5931 0.0064 1.6864 1.4511 0.0026 0.6811

4046 578 1.6690 0.0029 0.7309 1.6812 0.0029 0.7362 3.3158 0.0057 1.4521 1.1782 0.0020 0.5160

4047 608 1.9350 0.0032 0.9329 1.8670 0.0031 0.9001 3.8087 0.0063 1.8362 1.3182 0.0022 0.6355

4048 655 1.8074 0.0028 0.7686 1.8386 0.0028 0.7819 3.6364 0.0056 1.5464 1.4425 0.0022 0.6134

4049 534 1.6480 0.0031 0.7864 1.6113 0.0030 0.7689 3.2636 0.0061 1.5573 1.2141 0.0023 0.5793

4050 463 1.6948 0.0037 0.8261 1.6150 0.0035 0.7872 3.3129 0.0071 1.6149 1.0667 0.0023 0.5200

4051 551 1.8084 0.0033 0.8324 1.8410 0.0033 0.8474 3.6360 0.0066 1.6736 0.8900 0.0016 0.4097

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 467

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATI ON WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES

Anim al FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1026 596 1.9912 0.0033 0.9034 0.6269 0.0011 0.2844 0.6412 0.0011 0.2909 1.2402 0.0021 0.5627

1027 595 1.6469 0.0028 0.7198 0.7436 0.0012 0.3250 0.7397 0.0012 0.3233 1.4763 0.0025 0.6452

1028 554 1.2242 0.0022 0.5416 0.6839 0.0012 0.3025 0.7512 0.0014 0.3323 1.3579 0.0025 0.6007

1029 522 2.0439 0.0039 0.9919 0.6781 0.0013 0.3291 0.6742 0.0013 0.3272 1.3496 0.0026 0.6550

1030 574 1.6700 0.0029 0.7459 0.7653 0.0013 0.3418 0.7351 0.0013 0.3283 1.5009 0.0026 0.6703

1031 547 1.5901 0.0029 0.7629 0.6676 0.0012 0.3203 0.6184 0.0011 0.2967 1.2868 0.0024 0.6173

1032 621 1.4172 0.0023 0.6014 0.7334 0.0012 0.3112 0.8023 0.0013 0.3405 1.5344 0.0025 0.6512

1033 561 1.8686 0.0033 0.7991 0.7273 0.0013 0.3110 0.7151 0.0013 0.3058 1.4413 0.0026 0.6164

1034 512 1.9693 0.0038 0.9623 0.5679 0.0011 0.2775 0.5757 0.0011 0.2813 1.1448 0.0022 0.5594

1035 535 1.9474 0.0036 0.9434 0.7740 0.0014 0.3750 0.7168 0.0013 0.3473 1.4971 0.0028 0.7253

1036 597 2.7034 0.0045 1.2112 0.7566 0.0013 0.3390 0.7185 0.0012 0.3219 1.4455 0.0024 0.6476

1037 522 1.8864 0.0036 0.9000 0.6120 0.0012 0.2920 0.6408 0.0012 0.3057 1.2492 0.0024 0.5960

1038 582 2.3289 0.0040 1.1184 0.7701 0.0013 0.3698 0.7055 0.0012 0.3388 1.4664 0.0025 0.7042

1039 510 1.6997 0.0033 0.8090 0.6275 0.0012 0.2987 0.6298 0.0012 0.2998 1.2484 0.0024 0.5942

1040 661 2.0372 0.0031 0.9092 0.7811 0.0012 0.3486 0.8497 0.0013 0.3792 1.6089 0.0024 0.7180

1041 683 1.8921 0.0028 0.8647 0.6679 0.0010 0.3052 0.6946 0.0010 0.3174 1.3486 0.0020 0.6163

1042 687 2.1539 0.0031 0.9485 0.6861 0.0010 0.3021 0.6686 0.0010 0.2944 1.3447 0.0020 0.5921

1043 445 1.8358 0.0041 0.8465 0.7571 0.0017 0.3491 0.7503 0.0017 0.3460 1.5008 0.0034 0.6920

1044 635 1.9786 0.0031 0.9638 0.6915 0.0011 0.3368 0.6902 0.0011 0.3362 1.3677 0.0022 0.6662

1045 555 1.7824 0.0032 0.8868 0.6592 0.0012 0.3280 0.6985 0.0013 0.3475 1.3545 0.0024 0.6739

1046 594 2.3070 0.0039 1.0238 0.7106 0.0012 0.3154 0.7428 0.0013 0.3296 1.4110 0.0024 0.6262

1047 530 2.0412 0.0039 0.9750 0.7321 0.0014 0.3497 0.7514 0.0014 0.3589 1.4803 0.0028 0.7071

1048 625 1.8270 0.0029 0.8084 0.7523 0.0012 0.3329 0.7723 0.0012 0.3417 1.5026 0.0024 0.6649

1049 605 2.4220 0.0040 1.1178 0.8184 0.0014 0.3777 0.7337 0.0012 0.3386 1.5494 0.0026 0.7151

1050 597 1.6624 0.0028 0.7057 0.7205 0.0012 0.3058 0.6800 0.0011 0.2886 1.4068 0.0024 0.5972

1051 505 1.1790 0.0023 0.5851 0.6572 0.0013 0.3261 0.5699 0.0011 0.2828 1.2310 0.0024 0.6109

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 468

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

SEM.VES & CO.GL. E PIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES

Animal FINAL BODY

------------------------- ---------- --------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2026 505 1.5743 0.0031 0.7229 0.6916 0.0014 0.3176 0.6495 0.0013 0.2983 1.3370 0.0026 0.6140

2027 511 1.1878 0.0023 0.5189 0.7026 0.0014 0.3069 0.6810 0.0013 0.2975 1.3813 0.0027 0.6034

2028 567 2.0938 0.0037 0.9653 0.7844 0.0014 0.3616 0.8253 0.0015 0.3805 1.5779 0.0028 0.7275

2029 588 2.3799 0.0041 1.1202 0.8067 0.0014 0.3797 0.7917 0.0013 0.3727 1.5701 0.0027 0.7390

2030 625 2.1051 0.0034 0.9283 0.7894 0.0013 0.3481 0.6914 0.0011 0.3049 1.4708 0.0024 0.6486

2031 516 2.3005 0.0045 1.0849 0.6846 0.0013 0.3228 0.6554 0.0013 0.3091 1.3385 0.0026 0.6312

2032 492 1.7739 0.0036 0.8443 0.8290 0.0017 0.3946 0.7301 0.0015 0.3475 1.5541 0.0032 0.7397

2033 639 2.4335 0.0038 1.0999 0.6734 0.0011 0.3044 0.6630 0.0010 0.2997 1.3314 0.0021 0.6018

2034 608 2.1199 0.0035 0.9897 0.7231 0.0012 0.3376 0.7394 0.0012 0.3452 1.4605 0.0024 0.6819

2035 504 1.6221 0.0032 0.8004 0.6751 0.0013 0.3331 0.7346 0.0015 0.3625 1.4098 0.0028 0.6957

2036 491 1.6809 0.0034 0.7343 0.7494 0.0015 0.3274 0.7547 0.0015 0.3297 1.4823 0.0030 0.6475

2037 572 1.8144 0.0032 0.8104 0.7693 0.0013 0.3436 0.7425 0.0013 0.3316 1.4773 0.0026 0.6598

2038 540 2.5517 0.0047 1.1820 0.8472 0.0016 0.3924 0.8116 0.0015 0.3759 1.6193 0.0030 0.7501

2039 582 1.7870 0.0031 0.8159 0.7270 0.0012 0.3319 0.7817 0.0013 0.3569 1.5015 0.0026 0.685 6

2040 581 2.0468 0.0035 0.9749 0.7143 0.0012 0.3402 0.7383 0.0013 0.3517 1.4503 0.0025 0.6908

2041 614 1.5050 0.0025 0.6987 0.6854 0.0011 0.3182 0.6819 0.0011 0.3166 1.3568 0.0022 0.6299

2042 551 1.2961 0.0024 0.6253 0.7620 0.0014 0.3677 0.6562 0.0012 0.3166 1.4041 0.0026 0.6775

2043 640 1.3546 0.0021 0.6142 0.7383 0.0012 0.3347 1.4822 0.0023 0.6720

2044 674 1.8257 0.0027 0.8552 0.7243 0.0011 0.3393 0.7241 0.0011 0.3392 1.4317 0.0021 0.6706

2045 664 1.6518 0.0025 0.7749 0.7756 0.0012 0.3639 0.8130 0.0012 0.3814 1.5797 0.0024 0.7411

2046 563 1.5639 0.0028 0.7433 0.6716 0.0012 0.3192 0.6782 0.0012 0.3224 1.3470 0.0024 0.6402

2047 598 2.3298 0.0039 1.1008 0.7565 0.0013 0.3574 0.7166 0.0012 0.3386 1.4613 0.0024 0.6905

2048 495 1.8268 0.0037 0.8550 0.7508 0.0015 0.3514 0.6497 0.0013 0.3041 1.3899 0.0028 0.6505

2049 697 2.2349 0.0032 0.9940 0.8032 0.0012 0.3572 0.7712 0.0011 0.3430 1.5735 0.0023 0.6999

2050 606 1.9345 0.0032 0.8810 0.6150 0.0010 0.2801 0.6659 0.0011 0.3032 1.2516 0.0021 0.5700

2051 545 1.5891 0.0029 0.7298 0.6427 0.0012 0.2952 0.6348 0.0012 0.2915 1.2756 0.0023 0.5858

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 469

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPID IDYMIDES

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

3026 575 2.4182 0.0042 1.1482 0.6871 0.0012 0.3262 0.7432 0.0013 0.3529 1.4230 0.0025 0.6757

3027 485 1.8991 0.0039 0.9448 0.7503 0.0015 0.3733 0.7429 0.0015 0.3696 1.4951 0.0031 0.7438

3028 625 1.8973 0.0030 0.8447 0.7838 0.0013 0.3490 0.7725 0.0012 0.3439 1.5956 0.0026 0.7104

3029 611 2.3883 0.0039 1.1038 0.6996 0.0011 0.3233 0.6984 0.0011 0.3228 1.3928 0.0023 0.6437

3030 627 1.9354 0.0031 0.8876 0.8390 0.0013 0.3848 0.8147 0.0013 0.3736 1.6355 0.0026 0.7501

3031 524 1.9386 0.0037 0.9334 0.6610 0.0013 0.3182 0.6359 0.0012 0.3062 1.2939 0.0025 0.6230

3032 510 1.6818 0.0033 0.7830 0.6917 0.0014 0.3221 0.6894 0.0014 0.3210 1.3418 0.0026 0.6247

3033 587 2.6938 0.0046 1.2728 0.8131 0.0014 0.3842 0.7644 0.0013 0.3612 1.5583 0.0027 0.7363

3034 594 2.1860 0.0037 0.9623 0.7115 0.0012 0.3132 0.7308 0.0012 0.3217 1.4099 0.0024 0.6206

3035 624 1.2161 0.0019 0.5259 0.8112 0.0013 0.3508 0.8053 0.0013 0.3483 1.5870 0.0025 0.6863

3036 505 2.2141 0.0044 1.0476 0.7873 0.0016 0.3725 0.8849 0.0018 0.4187 1.6551 0.0033 0.7831

3037 577 1.9245 0.0033 0.8821 0.7387 0.0013 0.3386 0.7654 0.0013 0.3508 1.4826 0.0026 0.6796

3038 569 1.9414 0.0034 0.8840 0.8297 0.0015 0.3778 0.8247 0.0014 0.3755 1.6418 0.0029 0.7476

3039 568 1.6585 0.0029 0.7550 0.6849 0.0012 0.3118 0.7092 0.0012 0.3228 1.3853 0.0024 0.6306

3040 660 1.6262 0.0025 0.7587 0.7137 0.0011 0.3330 0.6658 0.0010 0.3106 1.3679 0.0021 0.6382

3041 629 1.8279 0.0029 0.8500 0.7670 0.0012 0.3567 0.7426 0.0012 0.3453 1.5053 0.0024 0.7000

3042 553 2.2894 0.0041 1.0786 0.8028 0.0015 0.3782 0.7604 0.0014 0.3582 1.5579 0.0028 0.7340

3045 476 1.9320 0.0041 0.8711 0.7408 0.0016 0.3340 0.7358 0.0015 0.3317 1.4820 0.0031 0.6682

3046 536 2.0079 0.0037 0.9292 0.7533 0.0014 0.3486 0.7906 0.0015 0.3658 1.5304 0.0029 0.7082

3047 559 1.9578 0.0035 0.9405 0.7413 0.0013 0.3561 0.7573 0.0014 0.3638 1.4945 0.0027 0.7180

3048 559 2.0985 0.0038 0.9975 0.8176 0.0015 0.3886 0.7500 0.0013 0.3565 1.5735 0.0028 0.7480

3049 550 2.0285 0.0037 0.9106 0.7298 0.0013 0.3276 0.7147 0.0013 0.3208 1.4578 0.0027 0.6544

3050 660 2.4739 0.0037 1.0398 0.7779 0.0012 0.3270 0.7514 0.0011 0.3158 1.5138 0.0023 0.6363

3051 646 1.6624 0.0026 0.7620 0.7684 0.0012 0.3522 0.7657 0.0012 0.3510 1.5449 0.0024 0.7081

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 470

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FI NAL BODY WEIGHTS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

SEM.VES & CO.GL. EPIDIDYMIS RIGHT EPIDIDYMIS LEFT EPIDIDYMIDES

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4026 642 2.0008 0.0031 0.9429 0.7907 0.0012 0.3726 0.7237 0.0011 0.3411 1.5060 0.0023 0.7097

4027 512 2.5156 0.0049 1.1526 0.6395 0.0012 0.2930 0.6410 0.0013 0.2937 1.3574 0.0027 0.6219

4028 597 2.2210 0.0037 1.0579 0.7286 0.0012 0.3470 0.6792 0.0011 0.3235 1.4035 0.0024 0.6685

4029 657 2.3201 0.0035 1.0374 0.8528 0.0013 0.3813 0.7951 0.0012 0.3555 1.6361 0.0025 0.7316

4030 591 2.1304 0.0036 0.9771 0.6953 0.0012 0.3189 0.6217 0.0011 0.2851 1.3098 0.0022 0.6007

4031 504 3.4055 0.0068 1.5419 0.7411 0.0015 0.3356 0.7113 0.0014 0.3221 1.4518 0.0029 0.6573

4032 783 2.4017 0.0031 1.1262 0.8013 0.0010 0.3758 0.7876 0.0010 0.3693 1.5583 0.0020 0.7307

4033 5 30 1.9443 0.0037 0.9293 0.8036 0.0015 0.3841 0.7698 0.0015 0.3679 1.5456 0.0029 0.7387

4034 553 1.6766 0.0030 0.8355 0.6797 0.0012 0.3387 0.7009 0.0013 0.3493 1.3578 0.0025 0.6766

4035 498 2.1931 0.0044 1.0968 0.7348 0.0015 0.3675 0.7814 0.0016 0.3908 1.4935 0.0030 0.7469

4036 596 2.0301 0.0034 0.9193 0.6817 0.0011 0.3087 0.7351 0.0012 0.3329 1.4138 0.0024 0.6402

4037 550 2.2986 0.0042 1.1291 0.7853 0.0014 0.3857 0.8463 0.0015 0.4157 1.6075 0.0029 0.7896

4038 520 2.0347 0.0039 0.9953 0.8352 0.0016 0.4086 0.7394 0.0014 0.3617 1.5515 0.0030 0.7 589

4039 552 2.3981 0.0043 1.1571 0.8748 0.0016 0.4221 0.8031 0.0015 0.3875 1.6796 0.0030 0.8104

4040 552 2.1016 0.0038 0.9883 0.7496 0.0014 0.3525 0.6718 0.0012 0.3159 1.4084 0.00 26 0.6623

4041 530 2.0509 0.0039 0.9748 0.7575 0.0014 0.3600 0.7721 0.0015 0.3670 1.5301 0.0029 0.7273

4042 595 1.6667 0.0028 0.7733 0.6949 0.0012 0.3224 0.7218 0.0012 0.3349 1.39 73 0.0023 0.6483

4043 521 2.0053 0.0038 0.9531 0.7071 0.0014 0.3361 0.7201 0.0014 0.3423 1.4236 0.0027 0.6766

4044 541 2.2020 0.0041 1.0420 0.6748 0.0012 0.3193 0.6763 0.0013 0.3200 1.3432 0.0025 0.6356

4045 566 2.3618 0.0042 1.1085 0.8331 0.0015 0.3910 0.7462 0.0013 0.3502 1.5759 0.0028 0.7397

4046 578 1.8491 0.0032 0.8098 0.7788 0.0013 0.3411 0.8385 0.0015 0.3672 1.5720 0.0027 0.6884

4047 608 2.3012 0.0038 1.1094 0.7390 0.0012 0.3563 0.7661 0.0013 0.3693 1.5110 0.0025 0.7285

4048 655 2.5843 0.0039 1.0990 0.7845 0.0012 0.3336 0.7874 0.0012 0.3349 1.5650 0.0024 0.6655

4049 534 1.9375 0.0036 0.9245 0.6646 0.0012 0.3171 0.6540 0.0012 0.3121 1.3228 0.0025 0.6312

4050 463 1.9974 0.0043 0.9736 0.6817 0.0015 0.3323 0.6496 0.0014 0.3166 1.3338 0.0029 0.6502

4051 551 2.1462 0.0039 0.9879 0.7516 0.0014 0.3460 0.7424 0.0013 0.3417 1.4740 0.0027 0.6785

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 471

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENS ATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Wei ght %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1026 596 0.0497 0.0001 0.0225 2.2042 0.0037 1.0000 4.5914 0.0077 2.0830 21.6371 0.0363 9.8163

1027 595 0.0672 0.0001 0.0294 2.2881 0.0038 1.0000 4.3794 0.0074 1.91 40 21.0723 0.0354 9.2095

1028 554 0.0622 0.0001 0.0275 2.2605 0.0041 1.0000 4.0830 0.0074 1.8062 19.5191 0.0352 8.6349

1029 522 0.0627 0.0001 0.0304 2.0606 0.0039 1.0000 3.5516 0.0068 1.7236 18.0607 0.0346 8.7648

1030 574 0.0607 0.0001 0.0271 2.2390 0.0039 1.0000 3.5564 0.0062 1.5884 19.1037 0.0333 8.5322

1031 547 0.0550 0.0001 0.0264 2.0844 0.0038 1.0000 3.9014 0.0071 1.8717 18.0676 0.0330 8.6680

1032 621 0.0563 0.0001 0.0239 2.3564 0.0038 1.0000 4.4814 0.0072 1.9018 23.1030 0.0372 9.8044

1033 561 0.0622 0.0001 0.0266 2.3383 0.0042 1.0000 4.2546 0.0076 1.8195 19.0090 0.0339 8.1294

1034 512 0.0540 0.0001 0.0264 2.0464 0.0040 1.0000 3.4090 0.0067 1.6659 16.0564 0.0314 7.8462

1035 535 0.0605 0.0001 0.0293 2.0642 0.0039 1.0000 3.3152 0.0062 1.6060 18.0211 0.0337 8.7303

1036 597 0.0585 0.0001 0.0262 2.2320 0.0037 1.0000 4.1916 0.0070 1.8780 19.2672 0.0323 8.6323

1037 522 0.0697 0.0001 0.0333 2.0959 0.0040 1.0000 3.5059 0.0067 1.6727 16.6953 0.0320 7.9657

1038 582 0.0655 0.0001 0.0315 2.0824 0.0036 1.0000 4.5715 0.0079 2.1953 20.3051 0.0349 9.7508

1039 510 0.0571 0.0001 0.0272 2.1010 0.0041 1.0000 3.7101 0.0073 1.7659 16.1684 0.0317 7.6956

1040 661 0.0552 0.0001 0.0246 2.2407 0.0034 1.0000 4.5382 0.0069 2.0253 21.8379 0.0330 9.7460

1041 683 0.0661 0.0001 0.0302 2.1881 0.0032 1.0000 4.9300 0.0072 2.2531 23.3785 0.0342 10.6844

1042 687 0.0650 0.0001 0.0286 2.2709 0.0033 1.0000 5.4274 0.0079 2.3900 23.0370 0.0335 10.1444

1043 445 0.0616 0.0001 0.0284 2.1687 0.0049 1.0000 3.4595 0.0078 1.5952 14.5009 0.0326 6.6864

1044 635 0.0788 0.0001 0.0384 2.0529 0.0032 1.0000 4.0483 0.0064 1.9720 22.4535 0.0354 10.9375

1045 555 0.0651 0.0001 0.0324 2.0099 0.0036 1.0000 3.7685 0.0068 1.8750 19.0286 0.0343 9.4674

1046 594 0.0672 0.0001 0.0298 2.2533 0.0038 1.0000 4.3610 0.0073 1.9354 20.1033 0.0338 8.9217

1047 530 0.0688 0.0001 0.0329 2.0935 0.0040 1.0000 3.8376 0.0072 1.8331 17.8346 0.0337 8.5190

1048 625 0.0803 0.0001 0.0355 2.2600 0.0036 1.0000 4.4123 0.0071 1.9523 18.8077 0.0301 8.3220

1049 605 0.0520 0.0001 0.0240 2.1668 0.0036 1.0000 4.2264 0.0070 1.9505 19.8124 0.0328 9.1436

1050 597 0.0608 0.0001 0.0258 2.3558 0.0039 1.0000 4.6326 0.0078 1.9665 19.6467 0.0329 8.3397

1051 505 0.0800 0.0002 0.0397 2.0152 0.0040 1.0000 3.7748 0.0075 1.8732 16.2320 0.0321 8.0548

----------------------------------------------------------------------------------------------------------------------------- ------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 472

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2026 505 0.0506 0.0001 0.0232 2.1777 0.0043 1.0000 3.7375 0.0074 1.7163 18.1728 0.0360 8.3450

2027 511 0.0551 0.0001 0.0241 2.2892 0.0045 1.0000 3.4106 0.0067 1.4899 16.4422 0.0322 7.1825

2028 567 0.0509 0.0001 0.0235 2.1690 0.0038 1.0000 4.1151 0.0073 1.8972 19.3291 0.0341 8.9115

2029 588 0.0491 0.0001 0.0231 2.1245 0.0036 1.0000 4.1381 0.0070 1.9478 19.5875 0.0333 9.2198

2030 6 25 0.0649 0.0001 0.0286 2.2677 0.0036 1.0000 4.7805 0.0076 2.1081 22.7354 0.0364 10.0258

2031 516 0.0753 0.0001 0.0355 2.1205 0.0041 1.0000 4.0010 0.0078 1.8868 18.1236 0.0351 8.5469

2032 492 0.0524 0.0001 0.0249 2.1011 0.0043 1.0000 3.3367 0.0068 1.5881 16.3219 0.0332 7.7683

2033 639 0.0654 0.0001 0.0296 2.2124 0.0035 1.0000 4.2193 0.0066 1.9071 20.8139 0.0326 9.4078

2034 608 0.0523 0.0001 0.0244 2.1419 0.0035 1.0000 4.2481 0.0070 1.9833 19.3562 0.0318 9.0369

2035 504 0.0518 0.0001 0.0256 2.0265 0.0040 1.0000 3.4495 0.0068 1.7022 15.4040 0.0306 7.6 013

2036 491 0.0674 0.0001 0.0294 2.2892 0.0047 1.0000 3.9796 0.0081 1.7384 17.3032 0.0352 7.5586

2037 572 0.0690 0.0001 0.0308 2.2390 0.0039 1.0000 3.7521 0.0066 1.6758 18.1371 0.03 17 8.1005

2038 540 0.0548 0.0001 0.0254 2.1588 0.0040 1.0000 4.5410 0.0084 2.1035 19.5089 0.0361 9.0369

2039 582 0.0721 0.0001 0.0329 2.1902 0.0038 1.0000 4.2684 0.0073 1.9489 19.85 77 0.0341 9.0666

2040 581 0.0670 0.0001 0.0319 2.0994 0.0036 1.0000 4.3133 0.0074 2.0545 19.6690 0.0339 9.3689

2041 614 0.0611 0.0001 0.0284 2.1539 0.0035 1.0000 4.0158 0.0065 1.8644 20.4904 0.0334 9.5132

2042 551 0.0860 0.0002 0.0415 2.0726 0.0038 1.0000 3.5861 0.0065 1.7302 18.8070 0.0342 9.0741

2043 640 0.0856 0.0001 0.0388 2.2056 0.0034 1.0000 4.1182 0.0064 1.8672 23.0512 0.0360 10.4512

2044 674 0.0567 0.0001 0.0266 2.1349 0.0032 1.0000 4.3578 0.0065 2.0412 23.4208 0.0348 10.9704

2045 664 0.0773 0.0001 0.0363 2.1315 0.0032 1.0000 4.4919 0.0068 2.1074 22.8447 0.0344 10.7177

2046 563 0.0678 0.0001 0.0322 2.1039 0.0037 1.0000 4.1426 0.0074 1.9690 18.7236 0.0333 8.8995

2047 598 0.0770 0.0001 0.0364 2.1164 0.0035 1.0000 4.0237 0.0067 1.9012 20.0965 0.0336 9.4956

2048 495 0.0676 0.0001 0.0316 2.1367 0.0043 1.0000 3.8509 0.0078 1.8023 16.9682 0.0343 7.9413

2049 697 0.0871 0.0001 0.0387 2.2483 0.0032 1 .0000 4.7940 0.0069 2.1323 24.4855 0.0352 10.8907

2050 606 0.0576 0.0001 0.0262 2.1959 0.0036 1.0000 4.2303 0.0070 1.9265 19.9556 0.0329 9.0877

2051 545 0.0575 0.0001 0.0264 2.1775 0.0040 1.0000 3.7397 0.0069 1.7174 18.7255 0.0343 8.5995

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 473

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

3026 575 0.0570 0.0001 0.0271 2.1061 0.0037 1.0000 4.6270 0.0080 2.1970 20.5960 0.0358 9.7792

3027 485 0.0921 0.0002 0.0458 2.0101 0.0041 1.0000 3.9876 0.0082 1.9838 17.0162 0.0351 8.4654

3028 625 0.0625 0.0001 0.0278 2.2461 0.0036 1.0000 4.6372 0.0074 2.0646 20.0811 0.0321 8.9404

3029 611 0.0625 0.0001 0.0289 2.1637 0.0035 1.0000 4.6139 0.0076 2.1324 21.0988 0.0345 9.7513

3030 627 0.0767 0.0001 0.0352 2.1804 0.0035 1.0000 5.3011 0.0085 2.4313 22.4215 0.0357 10.2832

3031 524 0.0580 0.0001 0.0279 2.0770 0.0040 1.0000 3.8281 0.0073 1.8431 17.1023 0.0326 8.2341

3032 510 0.0647 0.0001 0.0301 2.1478 0.0042 1.0000 3.8597 0.0076 1.7970 15.5536 0.0305 7.2416

3033 587 0.0696 0.0001 0.0329 2.1164 0.0036 1.0000 4.4308 0.0075 2.0936 21.1561 0.0360 9.9963

3034 594 0.0581 0.0001 0.0256 2.2717 0.0038 1.0000 4.4644 0.0075 1.9652 19.8822 0.0335 8.7521

3035 624 0.0577 0.0001 0.0250 2.3123 0.0037 1.0000 4.9814 0.0080 2.1543 23.3690 0.0374 10.1064

3036 505 0.0523 0.0001 0.0247 2.1135 0.0042 1.0000 4.0199 0.0080 1.9020 16.8945 0.0334 7.9936

3037 577 0.0595 0.0001 0.0273 2.1817 0.0038 1.0000 4.5452 0.0079 2.0833 21.1925 0.0367 9.7138

3038 569 0.0795 0.0001 0.0362 2.1962 0.0039 1.0000 5.0869 0.0089 2.3162 20.5248 0.0361 9.3456

3039 568 0.0642 0.0001 0.0292 2.1968 0.0039 1.0000 4.8341 0.0085 2.2005 22.2637 0.0392 10.1346

3040 660 0.0684 0.0001 0.0319 2.1435 0.0033 1.0000 5.2329 0.0079 2.4413 23.7361 0.0360 11.0735

3041 629 0.0748 0.0001 0.0348 2.1504 0.0034 1.0000 4.9062 0.0078 2.2815 23.3730 0.0372 10.8691

3042 553 0.0590 0.0001 0.0278 2.1226 0.0038 1.0000 4.8675 0.0088 2.2932 17.7070 0.0320 8.3421

3045 476 0.0610 0.0001 0.0275 2.2180 0.0047 1.0000 3.6753 0.0077 1.6570 15.9964 0.0336 7.2121

3046 536 0.0840 0.0002 0.0389 2.1610 0.0040 1.0000 4.4532 0.0083 2.0607 18.1863 0.0339 8.4157

3047 559 0.0714 0.0001 0.0343 2.0816 0.0037 1.0000 5.0694 0.0091 2.4353 23.6349 0.0423 11.3542

3048 559 0.0593 0.0001 0.0282 2.1037 0.0038 1.0000 4.3111 0.0077 2.0493 19.7936 0.0354 9.4089

3049 550 0.0679 0.0001 0.0305 2.2276 0.0041 1.0000 4.5017 0.0082 2.0209 19.8437 0.0361 8.9081

3050 660 0.1011 0.0002 0.0425 2.3791 0.0036 1.0000 5.3506 0.0081 2.2490 24.8219 0.0376 10.4333

3051 646 0.0716 0.0001 0.0328 2.1816 0.0034 1.0000 4.8972 0.0076 2.2448 21.3150 0.0330 9.770 4

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 474

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4026 642 0.0481 0.0001 0.0227 2.1219 0.0033 1.0000 4.9232 0.0077 2.3202 25.1112 0.0391 11.8343

4027 512 0.0529 0.0001 0.0242 2.1825 0.0043 1.0000 4.5250 0.0088 2.0733 18.7483 0.0366 8.5903

4028 597 0.0626 0.0001 0.0298 2.0995 0.0035 1.0000 4.5135 0.0076 2.1498 22.0398 0.0369 10.4976

4029 6 57 0.0602 0.0001 0.0269 2.2364 0.0034 1.0000 6.1342 0.0093 2.7429 23.0784 0.0351 10.3194

4030 591 0.0628 0.0001 0.0288 2.1803 0.0037 1.0000 4.8956 0.0083 2.2454 21.8050 0.0369 10.0009

4031 504 0.0593 0.0001 0.0268 2.2086 0.0044 1.0000 5.0211 0.0100 2.2734 20.4573 0.0406 9.2626

4032 783 0.0695 0.0001 0.0326 2.1325 0.0027 1.0000 5.8810 0.0075 2.7578 29.3185 0.0374 13.7484

4033 530 0.0608 0.0001 0.0291 2.0923 0.0039 1.0000 5.3645 0.0101 2.5639 20.4241 0.0385 9.7616

4034 553 0.0443 0.0001 0.0221 2.0068 0.0036 1.0000 4.0653 0.0074 2.0258 20.1266 0.0364 10.0 292

4035 498 0.0685 0.0001 0.0343 1.9996 0.0040 1.0000 4.8185 0.0097 2.4097 19.0169 0.0382 9.5104

4036 596 0.0574 0.0001 0.0260 2.2083 0.0037 1.0000 4.1563 0.0070 1.8821 21.2134 0.03 56 9.6062

4037 550 0.0463 0.0001 0.0227 2.0358 0.0037 1.0000 5.0785 0.0092 2.4946 20.5777 0.0374 10.1079

4038 520 0.0612 0.0001 0.0299 2.0443 0.0039 1.0000 4.5094 0.0087 2.2058 18.72 77 0.0360 9.1609

4039 552 0.0670 0.0001 0.0323 2.0725 0.0038 1.0000 4.5545 0.0083 2.1976 19.8811 0.0360 9.5928

4040 552 0.0743 0.0001 0.0349 2.1265 0.0039 1.0000 4.4954 0.0082 2.1140 23.6899 0.0430 11.1403

4041 530 0.0498 0.0001 0.0237 2.1039 0.0040 1.0000 4.9329 0.0093 2.3446 18.7611 0.0354 8.9173

4042 595 0.0571 0.0001 0.0265 2.1554 0.0036 1.0000 4.5598 0.0077 2.1155 23.7247 0.0399 11.0071

4043 521 0.0486 0.0001 0.0231 2.1039 0.0040 1.0000 4.1860 0.0080 1.9896 18.8542 0.0362 8.9615

4044 541 0.0675 0.0001 0.0319 2.1132 0.0039 1.0000 4.8299 0.0089 2.2856 21.7772 0.0403 10.3053

4045 566 0.0605 0.0001 0.0284 2.1306 0.0038 1.0000 4.6227 0.0082 2.1697 20.3016 0.0359 9.5286

4046 578 0.0590 0.0001 0.0258 2.2835 0.0040 1.0000 5.1649 0.0089 2.2618 20.9115 0.0362 9.1577

4047 608 0.0808 0.0001 0.0390 2.0742 0.0034 1.0000 4.9660 0.0082 2.3942 22.3447 0.0368 10.7727

4048 655 0.0913 0.0001 0.0388 2.3515 0.0036 1 .0000 4.3992 0.0067 1.8708 23.8447 0.0364 10.1402

4049 534 0.0568 0.0001 0.0271 2.0957 0.0039 1.0000 4.3855 0.0082 2.0926 20.3720 0.0381 9.7209

4050 463 0.0577 0.0001 0.0281 2.0515 0.0044 1.0000 3.6937 0.0080 1.8005 17.0722 0.0368 8.3218

4051 551 0.0541 0.0001 0.0249 2.1725 0.0039 1.0000 4.1878 0.0076 1.9276 19.6771 0.0357 9.0574

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 475

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN

Animal FINAL BODY

------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1026 596 1.6649 0.0028 0.7553 0.0125 0.0000 0.0057 0.7026 0.0012 0.3188

1027 595 1.6803 0.0028 0.7344 0.0128 0.0000 0.0056 0.7819 0.0013 0.3417

1028 554 1.6226 0.0029 0.7178 0.0110 0.0000 0.0049 0.8475 0.0015 0.3749

1029 522 1.7016 0.0033 0.8258 0.0107 0.0000 0.0052 0.7528 0.0014 0.3653

1030 574 1.8358 0.0032 0.8199 0.0121 0.0000 0.0054 0.7566 0.0013 0.3379

1031 547 1.6697 0.0031 0.8010 0.0109 0.0000 0.0052 0.7167 0.0013 0.3438

1032 621 2.4779 0.0040 1.0516 0.0151 0.0000 0 .0064 0.8986 0.0014 0.3813

1033 561 1.7288 0.0031 0.7393 0.0111 0.0000 0.0047 0.7179 0.0013 0.3070

1034 512 1.5607 0.0031 0.7627 0.0117 0.0000 0.0057 0.7868 0.0015 0.3845

1035 535 1.5183 0.0028 0.7355 0.0090 0.0000 0.0044 0.7235 0.0014 0.3505

1036 597 2.0919 0.0035 0.9372 0.0104 0.0000 0.0047 0.8550 0.0014 0.3831

1037 522 1.5740 0.0030 0.7510 0.0108 0.0000 0.0052 0.7675 0.0015 0.3662

1038 582 2.3818 0.0041 1.1438 0.0131 0.0000 0.0063 1.0208 0.0018 0.4902

1039 510 1.5953 0.0031 0.7593 0.0103 0.0000 0.0049 0.7961 0.0016 0.3789

1040 661 2.0305 0.0031 0.9062 0.0131 0.0000 0.0058 0.9771 0.0015 0.4361

1041 683 2.2176 0.0032 1.0135 0.0131 0.0000 0.0060 0.9468 0.0014 0.4327

1042 687 1.9694 0.0029 0.8672 0.0122 0.0000 0.0054 0.9194 0.0013 0.4049

1043 445 1.7349 0.0039 0.8000 0.0100 0.0000 0.0046 0.6989 0.0016 0.3223

1044 635 1.7016 0.0027 0.8289 0.0112 0.0000 0.0055 1.0277 0.0016 0.5006

1045 555 1.6095 0.0029 0.8008 0.0139 0.0000 0.0069 0.6809 0.0012 0.3388

1046 594 1.8767 0.0032 0.8329 0.0127 0.0000 0.0056 0.5907 0.0010 0.2621

1047 530 1.7311 0.0033 0.8269 0.0123 0.0000 0.0059 0.7426 0.0014 0.3547

10 48 625 1.8679 0.0030 0.8265 0.0145 0.0000 0.0064 1.2141 0.0019 0.5372

1049 605 1.9854 0.0033 0.9163 0.0109 0.0000 0.0050 0.7879 0.0013 0.3636

1050 597 2.2006 0.0037 0.9341 0.0137 0.0000 0.0058 0.6994 0.0012 0.2969

1051 505 1.9452 0.0038 0.9653 0.0117 0.0000 0.0058 0.7669 0.0015 0.3806

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Scie nces 00

- 4205 PAGE 476

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN

Animal FINAL BODY

------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2026 505 1.6806 0.0033 0.7717 0.0066 0.0000 0.0030 0.7052 0.0014 0.3238

2027 511 1.4735 0.0029 0.6437 0.0103 0.0000 0.0045 0.6373 0.0012 0.2784

2028 567 1.6954 0.0030 0.7817 0.0055 0.0000 0.0025 0.8434 0.0015 0.3888

2029 588 1.9105 0.0033 0.8993 0.0130 0.0000 0.0061 0.8114 0.0014 0.3819

2030 625 2.0506 0.0033 0.9043 0.0116 0.0000 0.0051 0.9337 0.0015 0.4117

2031 516 1.5817 0.0031 0.7459 0.0109 0.0000 0.0051 0.7684 0.0015 0.3624

20 32 492 1.5945 0.0032 0.7589 0.0087 0.0000 0.0041 0.7208 0.0015 0.3431

2033 639 1.9385 0.0030 0.8762 0.0115 0.0000 0.0052 0.7357 0.0012 0.3325

2034 608 1.7966 0.0030 0.8388 0.0125 0.0000 0.0058 0.9700 0.0016 0.4529

2035 504 1.5852 0.0031 0.7822 0.0127 0.0000 0.0063 0.6600 0.0013 0.3257

2036 491 1.9539 0.0040 0.8535 0.0118 0.0000 0.0052 0.7581 0.0015 0.3312

2037 572 1.8430 0.0032 0.8231 0.0117 0.0000 0.0052 0.6169 0.0011 0.2755

2038 540 1.5982 0.0030 0.7403 0.0101 0.0000 0.0047 0.9692 0.0018 0.4490

2039 582 1.8116 0.0031 0.8271 0.0103 0.0000 0.0047 0.6941 0.0012 0.3169

2040 581 1.8326 0.0032 0.8729 0.0123 0.0000 0.0059 0.7065 0.0012 0.3365

2041 614 1.8902 0.0031 0.8776 0.0109 0.0000 0.0051 0.7968 0.0013 0. 3699

2042 551 1.7207 0.0031 0.8302 0.0108 0.0000 0.0052 0.6609 0.0012 0.3189

2043 640 2.0147 0.0031 0.9134 0.0112 0.0000 0.0051 0.8252 0.0 013 0.3741

2044 674 1.8177 0.0027 0.8514 0.0121 0.0000 0.0057 0.6028 0.0009 0.2824

2045 664 1.8537 0.0028 0.8697 0.0138 0.0000 0.0065 0.7 125 0.0011 0.3343

2046 563 1.9157 0.0034 0.9105 0.0121 0.0000 0.0058 0.8378 0.0015 0.3982

2047 598 1.7662 0.0030 0.8345 0.0112 0.0000 0.005 3 0.8314 0.0014 0.3928

2048 495 1.7010 0.0034 0.7961 0.0131 0.0000 0.0061 0.9375 0.0019 0.4388

2049 697 2.3427 0.0034 1.0420 0.0152 0.0000 0.0068 0.7549 0.0011 0.3358

2050 606 1.7826 0.0029 0.8118 0.0139 0.0000 0.0063 0.7247 0.0012 0.3300

2051 545 1.9435 0.0036 0.8925 0.0120 0.0000 0.0055 0.7855 0.0014 0.3607

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 477

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY S TUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN

Animal FINAL BODY

------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

3026 575 1.6761 0.0029 0.7958 0.0105 0.0000 0.0050 0.7205 0.0013 0.3421

3027 485 1.5179 0.0031 0.7551 0.0107 0.0000 0.0053 0.7227 0.0 015 0.3595

3028 625 2.1264 0.0034 0.9467 0.0121 0.0000 0.0054 0.6807 0.0011 0.3031

3029 611 1.7633 0.0029 0.8149 0.0119 0.0000 0.0055 0.7 797 0.0013 0.3604

3030 627 2.0062 0.0032 0.9201 0.0121 0.0000 0.0055 1.0044 0.0016 0.4606

3031 524 1.6220 0.0031 0.7809 0.0122 0.0000 0.005 9 0.7442 0.0014 0.3583

3032 510 1.6470 0.0032 0.7668 0.0134 0.0000 0.0062 0.8738 0.0017 0.4068

3033 587 2.2473 0.0038 1.0619 0.0115 0.0000 0.0054 0.8757 0.0015 0.4138

3034 594 1.7066 0.0029 0.7512 0.0127 0.0000 0.0056 0.7063 0.0012 0.3109

3035 624 1.9884 0.0032 0.8599 0.0106 0.0000 0.0046 0.7755 0.0012 0.3354

3036 505 1.6280 0.0032 0.7703 0.0094 0.0000 0.0044 0.7708 0.0015 0.3647

3037 577 1.8304 0.0032 0.8390 0.0110 0.0000 0.0050 0.8814 0.0015 0.4040

3038 569 2.2100 0.0039 1.0063 0.0123 0.0000 0.0056 0.8817 0.0015 0.4015

3039 568 1.7922 0.0032 0.8158 0.0123 0.0000 0.0056 0.9555 0.0017 0.4350

3040 660 1.8278 0.0028 0.8527 0.0115 0.0000 0.0054 1.0728 0.0016 0.5005

3041 629 1.7134 0.0027 0.7968 0.0139 0.0000 0.0065 1.0363 0.0016 0.4819

3042 553 1.8185 0.0033 0.8567 0.0107 0.0000 0.0050 0.7856 0.0014 0.3701

3045 476 1.7899 0.0038 0.8070 0.0105 0.0000 0.0047 0.5933 0.0012 0.2675

3046 536 1.8109 0.0034 0.8380 0.0116 0.0000 0.0054 0.8835 0.0016 0.4088

3047 5 59 1.8809 0.0034 0.9036 0.0113 0.0000 0.0054 0.8246 0.0015 0.3961

3048 559 1.7809 0.0032 0.8466 0.0124 0.0000 0.0059 0.9210 0.0016 0.4378

3049 550 1.9036 0.0035 0.8546 0.0130 0.0000 0.0058 0.8421 0.0015 0.3780

3050 660 2.2453 0.0034 0.9438 0.0145 0.0000 0.0061 1.0414 0.0016 0.4377

3051 646 2.2972 0.0036 1.0530 0.0149 0.0000 0.0068 0.7781 0.0012 0.3567

----------------------------------------------------------------------------------------------------------------------------- - -----

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 478

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

MALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN

Animal FINAL BODY

------------------------- ------------------------- ---------------- ---------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4026 642 2.0399 0.0032 0.9614 0.0118 0.0000 0.0056 0.8034 0.0013 0.3786

4027 512 1.7243 0.0034 0.7901 0.0123 0.0000 0.0056 0.6395 0.0012 0.2930

4028 597 1.6918 0.0028 0.8058 0.0107 0.0000 0.0051 0.7258 0.0012 0.3457

4029 657 1.9865 0.0030 0.8883 0.0097 0.0000 0.0043 0.7905 0.0012 0.3535

4030 591 1.5825 0.0027 0.7258 0.0140 0.0000 0.0064 0.8130 0.0014 0.3729

4031 5 04 2.0731 0.0041 0.9386 0.0160 0.0000 0.0072 0.8205 0.0016 0.3715

4032 783 2.0094 0.0026 0.9423 0.0108 0.0000 0.0051 1.1075 0.0014 0.5193

4033 530 1.7040 0.0032 0.8144 0.0119 0.0000 0.0057 0.7246 0.0014 0.3463

4034 553 1.8835 0.0034 0.9386 0.0109 0.0000 0.0054 0.7353 0.0013 0.3664

4035 498 1.7434 0.0035 0.8719 0.0119 0.0000 0.0060 0.7869 0.0016 0.3935

4036 596 1.8743 0.0031 0.8488 0.0127 0.0000 0.0058 0.8058 0.0014 0.3649

4037 550 1.8141 0.0033 0.8911 0.0070 0.0000 0.0034 0.8321 0.0015 0.4087

4038 520 1.7147 0.0033 0.8388 0.0124 0.0000 0.0061 0.8396 0.0016 0.4107

4039 552 1.8113 0.0033 0.8740 0.0105 0.0000 0.0051 0.6913 0.0013 0.3336

4040 552 1.9324 0.0035 0.9087 0.0126 0.0000 0.0059 0.7671 0.0014 0.3607

4041 530 1.6449 0.0031 0.7818 0.0118 0.0000 0.0056 0.8959 0.0017 0.4258

4042 595 1.7210 0.0029 0.7985 0.0090 0.0000 0.0042 0.7000 0.0012 0.3248

4043 521 1.6643 0.0032 0.7911 0.0083 0.0000 0.0039 0.6125 0.0012 0.2911

4044 541 1.9595 0.0036 0.9273 0.0094 0.0000 0.0044 0.8839 0.0016 0.4183

4045 566 2.0707 0.0037 0.9719 0.0136 0.0000 0.0064 0.8277 0.0015 0.3885

4046 578 1.7913 0.0031 0.7845 0.0123 0.0000 0.0054 0.7890 0.0014 0.3455

4047 608 1.8242 0.0030 0.8795 0.0148 0.0000 0.0071 0.6940 0.0011 0.3346

4048 655 2.0407 0.0031 0.8678 0.0145 0.0000 0.0062 0.9682 0.0015 0.4117

4049 534 1.8180 0.0034 0.8675 0.0133 0.0000 0.0063 0.7544 0.0014 0.3600

4050 463 1.4950 0.0032 0.7287 0.0110 0.0000 0 .0054 0.6562 0.0014 0.3199

4051 551 1.7979 0.0033 0.8276 0.0131 0.0000 0.0060 0.8929 0.0016 0.4110

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 479

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1526 268 0.0690 0.0003 0.0357 1.9342 0.0072 1.0000 2.2961 0.0086 1.1871 11.7388 0.0438 6.0691

1527 306 0.0656 0.0002 0.0353 1.8588 0.0061 1.0000 2.7035 0.0088 1.4544 14.5085 0.0475 7.8053

1528 269 0.0709 0.0003 0.0360 1.9697 0.0073 1.0000 2.2505 0.0084 1.1426 11.3636 0.0423 5.7692

1529 309 0.0720 0.0002 0.0358 2.0096 0.0065 1.0000 2.3786 0.0077 1.1836 12.8991 0.0417 6.4187

1530 327 0.0685 0.0002 0.0356 1.9232 0.0059 1.0000 2.5788 0.0079 1.3409 13.7154 0.0419 7.1316

1531 295 0.0675 0.0002 0.0359 1.8810 0.0064 1.0000 2.2593 0.0076 1.2011 12.7914 0.0433 6.8003

1532 316 0.0675 0.0002 0.0358 1.8852 0.0060 1.0000 2.8307 0.0090 1.5015 14.9345 0.0473 7.9220

1533 329 0.0709 0.0002 0.0350 2.0271 0.0062 1.0000 2.7475 0.0083 1.3554 14.0334 0.0426 6.9229

1535 311 0.0693 0.0002 0.0358 1.9345 0.0062 1.0000 2.5821 0.0083 1.3348 14.2344 0.0458 7.3582

1536 331 0.0738 0.0002 0.0366 2.0152 0.0061 1.0000 2.6592 0.0080 1.3196 13.8851 0.0419 6.8902

1537 342 0.0746 0.0002 0.0368 2.0276 0.0059 1.0000 2.6700 0.0078 1.3168 16.7770 0.0491 8.2743

1539 339 0.0887 0.0003 0.0446 1.9881 0.0059 1.0000 2.7608 0.0081 1.3887 16.8518 0.0497 8.4763

1541 335 0.0839 0.0003 0.0412 2.0364 0.0061 1.0000 2.7186 0.0081 1.3350 16.4748 0.0493 8.0902

1542 300 0.0802 0.0003 0.0433 1.8543 0.0062 1.0000 2.7735 0.0092 1.4957 14.5041 0.0483 7.8219

1543 311 0.0947 0.0003 0.0536 1.7676 0.0057 1.0000 2.5818 0.0083 1.4606 13.3808 0.0431 7.5700

1544 344 0.0773 0.0002 0.0401 1.9268 0.0056 1.0000 2.7489 0.0080 1.4267 16.2738 0.0473 8.4460

1545 284 0.0673 0.0002 0.0344 1.9579 0.0069 1.0000 2.4160 0.0085 1.2340 13.3421 0.0469 6.8145

1546 304 0.0678 0.0002 0.0340 1.9927 0.0066 1.0000 2.6363 0.0087 1.3230 14.1209 0.0464 7.0863

1547 291 0.0669 0.0002 0.0346 1.9363 0.0066 1.0000 2.3435 0.0080 1.2103 12.3125 0.0423 6.3588

1550 268 0.0736 0.0003 0.0364 2.0198 0.0075 1.0000 2.4009 0.0090 1.1887 11.2886 0.0422 5.5890

1551 292 0.0706 0.0002 0.0394 1.7897 0.0061 1.0000 2.5342 0.0087 1.4160 12.8911 0.0441 7.2029

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 480

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FI NAL BODY WEIGHTS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2526 303 0.0983 0.0003 0.0521 1.8875 0.0062 1.0000 2.4309 0.0080 1.2879 12.5073 0.0412 6.6264

2527 291 0.0848 0.0003 0.0448 1.8927 0.0065 1.0000 2.4557 0.0085 1.2975 12.5833 0.0433 6.6483

2528 330 0.0909 0.0003 0.0460 1.9754 0.0060 1.0000 2.9181 0.0088 1.4772 15.1089 0.0458 7.6485

2529 292 0.0681 0.0002 0.0360 1.8901 0.0065 1.0000 2.5470 0.0087 1.3475 12.4484 0.0427 6.5861

2530 291 0.0795 0.0003 0.0417 1.9076 0.0066 1.0000 2.5260 0.0087 1.3242 12.9113 0.0444 6.7683

2531 321 0.0723 0.0002 0.0378 1.9146 0.0060 1.0000 2.6537 0.0083 1.3860 14.8639 0.0462 7.7634

2532 309 0.0616 0.0002 0.0345 1.7866 0.0058 1.0000 2.6984 0.0087 1.5104 13.5693 0.0439 7.5950

2533 3 66 0.0724 0.0002 0.0353 2.0527 0.0056 1.0000 2.9310 0.0080 1.4279 16.4653 0.0450 8.0213

2534 326 0.0772 0.0002 0.0411 1.8774 0.0058 1.0000 2.6450 0.0081 1.4089 14.1727 0.0435 7.5491

2535 299 0.0619 0.0002 0.0364 1.7014 0.0057 1.0000 2.2113 0.0074 1.2997 12.8387 0.0430 7.5460

2537 348 0.0652 0.0002 0.0326 2.0006 0.0057 1.0000 2.7179 0.0078 1.3585 13.7327 0.0394 6.8643

2538 343 0.0783 0.0002 0.0381 2.0552 0.0060 1.0000 2.3884 0.0070 1.1621 12.9122 0.0376 6.2827

2539 315 0.0733 0.0002 0.0359 2.0419 0.0065 1.0000 2.5320 0.0080 1.2400 15.2936 0.0486 7.4 899

2540 278 0.0697 0.0003 0.0368 1.8950 0.0068 1.0000 2.5245 0.0091 1.3322 12.3091 0.0442 6.4956

2541 310 0.0668 0.0002 0.0347 1.9250 0.0062 1.0000 2.5505 0.0082 1.3249 13.8606 0.04 47 7.2003

2542 298 0.0829 0.0003 0.0435 1.9049 0.0064 1.0000 2.6618 0.0089 1.3973 12.8159 0.0430 6.7279

2543 PNMD 322 0.0700 0.0002 0.0370 1.8915 0.0059 1.0000 2.6686 0.0083 1.4108 16.42 46 0.0510 8.6834

2545 331 0.0711 0.0002 0.0367 1.9371 0.0059 1.0000 2.6877 0.0081 1.3875 14.0572 0.0425 7.2568

2546 314 0.1003 0.0003 0.0526 1.9068 0.0061 1.0000 2.6617 0.0085 1.3959 16.1563 0.0515 8.4730

2547 265 0.0784 0.0003 0.0388 2.0203 0.0076 1.0000 2.2106 0.0083 1.0942 9.0096 0.0339 4.4595

2548 308 0.0631 0.0002 0.0316 1.9982 0.0065 1.0000 2.5685 0.0083 1.2854 13.9637 0.0453 6.9881

2550 293 0.0604 0.0002 0.0341 1.7726 0.0060 1.0000 2.6035 0.0089 1.4687 13.9971 0.0477 7.8964

2551 327 0.0802 0.0002 0.0408 1.9680 0.0060 1.0000 2.8744 0.0088 1.4606 16.5279 0.0505 8.3983

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PR EGNANT, NO MATING DATE11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 481

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP III 10000 MG/M3

------------------------------------------------------------------------------------------------------------------------ -----------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %F BW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

3527 303 0.1072 0.0004 0.0549 1.9514 0.0064 1.0000 2.4618 0.0081 1.2616 14.2889 0.0471 7.3224

3528 355 0.0871 0.0002 0.0434 2.0072 0.0057 1.0000 2.7306 0.0077 1.3604 14.6180 0.0412 7.2828

3529 287 0.0854 0.0003 0.0456 1.8720 0.0065 1.0000 2.5469 0.0089 1.3605 12.6058 0.0439 6.7339

3531 310 0.0863 0.0003 0.0458 1.8842 0.0061 1.0000 2.8295 0.0091 1.5 017 15.5423 0.0501 8.2488

3532 297 0.0662 0.0002 0.0348 1.9042 0.0064 1.0000 2.4502 0.0083 1.2867 12.5695 0.0423 6.6009

3533 305 0.0806 0.0003 0.0409 1.9717 0.0065 1.0000 2.4181 0.0079 1.2264 13.7093 0.0449 6.9530

3535 275 0.0682 0.0002 0.0373 1.8270 0.0066 1.0000 2.5479 0.0093 1.3946 11.5774 0.0421 6.3368

3536 304 0.0698 0.0002 0.0345 2.0254 0.0067 1.0000 2.7019 0.0089 1.3340 15.0901 0.0496 7.4504

3537 288 0.0594 0.0002 0.0297 1.9994 0.0069 1.0000 2.6672 0.0093 1.3340 12.6084 0.0438 6.3061

3538 326 0.0700 0.0002 0.0363 1.9281 0.0059 1.0000 2.6460 0.0081 1.3723 16.3096 0.0501 8.4589

3539 343 0.0650 0.0002 0.0328 1.9800 0.0058 1.0000 2.7390 0.0080 1.3833 17.4218 0.0508 8.7989

3540 320 0.0540 0.0002 0.0276 1.9589 0.0061 1.0000 2.8679 0.0090 1.4640 16.6089 0.0519 8.4787

3541 339 0.0584 0.0002 0.0301 1.9415 0.0057 1.0000 2.5620 0.0076 1.3196 16.6451 0.0491 8.5733

3542 316 0.0736 0.0002 0.0380 1.9367 0.0061 1.0000 2.8326 0.0090 1.4626 13.4752 0.0426 6.9578

3543 291 0.0637 0.0002 0.0342 1.8631 0.0064 1.0000 2.3508 0.0081 1.2618 13.2622 0.0456 7.1184

3544 287 0.0615 0.0002 0.0351 1.7543 0.0061 1.0000 2.4389 0.0085 1.3902 12.9773 0.0453 7.3974

3545 336 0.0649 0.0002 0.0338 1.9219 0.0057 1.0000 3.0667 0.0091 1.5957 15.1961 0.0452 7.9068

3546 335 0.0590 0.0002 0.0306 1.9307 0.0058 1.0000 2.6194 0.0078 1.3567 14.3649 0.0429 7.4403

3547 275 0.0648 0.0002 0.0342 1.8942 0.0069 1.0000 2.2186 0.0081 1.1713 12.2604 0.0446 6.4726

3548 319 0.0736 0.0002 0.0382 1.9275 0.0060 1.0000 2.8445 0.0089 1.4757 14.4653 0.0454 7.5047

3549 289 0.0833 0.0003 0.0421 1.9794 0.0069 1.0000 2.6258 0.0091 1.3266 13.5990 0.0471 6.8703

3550 309 0.0944 0.0003 0.0481 1.9607 0.0064 1.0000 2.7409 0.0089 1.3979 15.0784 0.0489 7.6903

3551 318 0.0771 0.0002 0.0407 1.8955 0.0060 1.0000 2.7704 0.0087 1.4616 15.7650 0.0497 8.3171

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 482

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BOD Y WEIGHTS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

ADRENALS BRAIN KIDNEYS LIVER

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4526 288 0.0824 0.0003 0.0415 1.9867 0.0069 1.0000 2.5238 0.0088 1.2703 13.0964 0.0456 6.5920

4527 306 0.0647 0.0002 0.0321 2.0171 0.0066 1.0000 2.4326 0.0080 1.2060 14.5006 0.0474 7.1888

4528 308 0.0809 0.0003 0.0398 2.0308 0.0066 1.0000 2.9558 0.0096 1.4555 12.7873 0.0416 6.2967

4529 353 0.1056 0.0003 0.0527 2.0034 0.0057 1.0000 3.1637 0.0090 1.5792 16.2706 0.0461 8.1215

4530 PNMD 307 0.0839 0.0003 0.0462 1.8168 0.0059 1.0000 2.8369 0.0092 1.5615 13.3414 0.0434 7.3434

4531 317 0.0824 0.0003 0.0419 1.9667 0.0062 1.0000 2.8388 0.0089 1.4434 12.9324 0.0408 6.5757

4532 300 0.0718 0.0002 0.0383 1.8751 0.0063 1.0000 2.4919 0.0083 1.3289 13.2965 0.0443 7.0911

4533 303 0.0704 0.0002 0.0367 1.9170 0.0063 1.0000 2.5105 0.0083 1.3096 14.5237 0.0480 7.5763

4534 300 0.0629 0.0002 0.0339 1.8569 0.0062 1.0000 2.8176 0.0094 1.5174 13.2892 0.0443 7.1567

4535 352 0.0694 0.0002 0.0340 2.0384 0.0058 1.0000 2.8218 0.0080 1.3843 18.1790 0.0517 8.9183

4536 345 0.0765 0.0002 0.0391 1.9558 0.0057 1.0000 2.8459 0.0082 1.4551 17.9329 0.0519 9.1691

4537 312 0.0813 0.0003 0.0430 1.8927 0.0061 1.0000 2.7381 0.0088 1.4467 14.6032 0.0468 7.7155

4539 300 0.0734 0.0002 0.0399 1.8382 0.0061 1.0000 2.6265 0.0087 1.4288 12.8644 0.0428 6.9984

4540 359 0.0800 0.0002 0.0388 2.0628 0.0057 1.0000 3.0311 0.0084 1.4694 18.2552 0.0508 8.8497

4541 290 0.0916 0.0003 0.0474 1.9328 0.0067 1.0000 2.5288 0.0087 1.3084 12.6979 0.0439 6. 5697

4542 268 0.0675 0.0003 0.0360 1.8728 0.0070 1.0000 2.2435 0.0084 1.1979 12.3581 0.0462 6.5987

4545 293 0.0801 0.0003 0.0434 1.8446 0.0063 1.0000 2.5420 0.0087 1.3781 14.5665 0.0497 7.8968

4546 352 0.0690 0.0002 0.0359 1.9232 0.0055 1.0000 2.7978 0.0080 1.4548 16.7635 0.0477 8.7165

4547 365 0.0923 0.0003 0.0450 2.0506 0.0056 1.0000 3.1394 0.0086 1.5310 18.1232 0.0496 8.8380

4548 271 0.0680 0.0003 0.0357 1.9051 0.0070 1.0000 2.6210 0.0097 1.3758 10.9666 0.0405 5.7564

4549 280 0.0702 0.0003 0.0355 1.9768 0.0070 1.0000 2.4548 0.0088 1.241 8 13.9113 0.0496 7.0373

4550 282 0.0627 0.0002 0.0329 1.9051 0.0068 1.0000 2.3135 0.0082 1.2144 13.8635 0.0492 7.2770

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 483

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP I 0 MG/M3

-------------------------------------------------------------------------------------------------------------------------------- ---

LUNGS PITUITARY SPLEEN OVARY RIGHT

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1526 268 1.3146 0.0049 0.6797 0.0139 0.0001 0.0072 0.4915 0.0018 0.2541 0.0507 0.0002 0.0262

1527 306 1.2909 0.0042 0.6945 0.0121 0.0000 0.0065 0.6723 0.0022 0.3617 0.0329 0.0001 0.0177

1528 269 1.3974 0.0052 0.7094 0.0146 0.0001 0.0074 0.6331 0.0024 0.3214 0.0751 0.0003 0.0381

1529 309 1.4990 0.0048 0.7459 0.0129 0.0000 0.0064 0.4276 0.0014 0.2128 0.0670 0.0002 0.0333

1530 327 1.4945 0.0046 0.7771 0.0187 0.0001 0.0097 0.5916 0.0018 0.3076 0.0551 0.0002 0.0287

1531 295 1.4090 0.0048 0.7491 0.0153 0.0001 0.0081 0.4450 0.0015 0.2366 0.0612 0.0002 0.0325

1532 316 1.4956 0.0047 0.7933 0.0151 0.0000 0.0080 0.6370 0.0020 0.3379 0.0495 0.0002 0.0263

1533 329 1.5628 0.0047 0.7710 0.0178 0.0001 0.0088 0.5645 0.0017 0.2785 0.0590 0.0002 0.0291

1535 311 1.4794 0.0048 0.7647 0.0154 0.0000 0.0080 0.6440 0.0021 0.3329 0.0521 0.0002 0.0269

1536 331 1.4262 0.0043 0.7077 0.0156 0.0000 0.0077 0.7043 0.0021 0.3495 0.0515 0.0002 0.0256

1537 342 1.5461 0.0045 0.7625 0.0070 0.0000 0.0035 0.5333 0.0016 0.2630 0.0459 0.0001 0.0226

1539 339 1.6031 0.0047 0.8063 0.0137 0.0000 0.0069 0.6873 0.0020 0.3457 0.0785 0.0002 0.0395

1541 335 1.6498 0.0049 0.8102 0.0138 0.0000 0.0068 0.6265 0.0019 0.3077 0.0678 0.0002 0.0333

1542 300 1.6510 0.0055 0.8904 0.0153 0.0001 0.0083 0.6142 0.0020 0.3312 0.0695 0.0002 0.0375

1543 311 1.3524 0.0044 0.7651 0.0161 0.0001 0.0091 0.6290 0.0020 0.3558 0.0797 0.0003 0.0451

1544 344 1.5239 0.0044 0.7909 0.0175 0.0001 0.0091 0.6493 0.0019 0.3370 0.0578 0.0002 0.0300

1545 284 1.4049 0.0049 0.7176 0.0085 0.0000 0.0043 0.5663 0.0020 0.2892 0.0507 0.0002 0.0259

1546 304 1.3226 0.0043 0.6637 0.0144 0.0000 0.0072 0.5174 0.0017 0.2596 0.0587 0.0002 0.0295

1547 291 1.3949 0.0048 0.7204 0.0155 0.0001 0.0080 0.6403 0.0022 0.3307 0.0479 0.0002 0.0247

1550 268 1.4211 0.0053 0.7036 0.0141 0.0001 0.0070 0.4723 0.0018 0.2338 0.0543 0.0002 0.0269

1551 292 1.3189 0.0045 0.7369 0.0137 0.0000 0.0077 0.5777 0.0020 0.3228 0.0474 0.0002 0.0265

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 484

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODU CTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN OVARY RIGHT

Animal FINAL BODY

------------------------- ------------------------- --- ---------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2526 303 1.5215 0.0050 0.8061 0.0198 0.0001 0.0105 0.5793 0.0019 0.3069 0.0673 0.0002 0.0357

2527 291 1.5100 0.0052 0.7978 0.0172 0.0001 0.0091 0.5762 0.0020 0.3044 0.0703 0.0002 0.0371

2528 330 1.3997 0.0042 0.7086 0.0204 0.0001 0.0103 0.6536 0.0020 0.3309 0.0494 0.0001 0.0250

2529 292 1.4160 0.0049 0.7492 0.0161 0.0001 0.0085 0.5114 0.0018 0.2706 0.0409 0.0001 0.0216

2530 291 1.4907 0.0051 0.7815 0.0198 0.0001 0.0104 0.5916 0.0020 0.3101 0.0640 0.0002 0.0336

2531 321 1.3404 0.0042 0.7001 0.0164 0.0001 0.0086 0.6127 0.0019 0.3200 0.0426 0.0001 0.0223

2532 309 1.2775 0.0041 0.7150 0.0149 0.0000 0.0083 0.5357 0.0017 0.2998 0.0529 0.0002 0.0296

2533 366 1.6978 0.0046 0.8271 0.0058 0.0000 0.0028 0.7501 0.0021 0.3654 0.0904 0.0002 0.0440

2534 326 1.4532 0.0045 0.7740 0.0148 0.0000 0.0079 0.6604 0.0020 0.3518 0.0379 0.0001 0.0202

2535 299 1.2094 0.0040 0.7108 0.0141 0.0000 0.0083 0.5664 0.0019 0.3329 0.0474 0.0002 0.0279

2537 348 1.7359 0.0050 0.8677 0.0165 0.0000 0.0082 0.6482 0.0019 0.3240 0.0629 0.0002 0.0314

2538 343 1.3408 0.0039 0.6524 0.0213 0.0001 0.0104 0.5947 0.0017 0.2894 0.0516 0.0002 0.0251

2539 315 1.4580 0.0046 0.7140 0.0167 0.0001 0.0082 0.5585 0.0018 0.2735 0.0477 0.0002 0.0234

2540 278 1.4775 0.0053 0.7797 0.0099 0.0000 0.0052 0.5794 0.0021 0.3058 0.0424 0.0002 0.0224

2541 310 1.5219 0.0049 0.7906 0.0132 0.0000 0.0069 0.5416 0.0017 0.2814 0.0559 0.0002 0.0290

2542 298 1.3924 0.0047 0.7310 0.0175 0.0001 0.0092 0.5906 0.0020 0.3100 0.0706 0.0002 0.0371

2543 PNMD 322 1.4711 0.0046 0.7777 0.0144 0.0000 0.0076 0.5190 0.0016 0.2744 0.0462 0.0001 0.0244

2545 331 1.4575 0.0044 0.7524 0.0157 0.0000 0.0081 0.6932 0.0021 0.3579 0.0649 0.0002 0.0335

2546 314 1.5393 0.0049 0.8073 0.0148 0.0000 0.0078 0.6631 0.0021 0.3478 0.0571 0.0002 0.0299

2547 265 1.4163 0.0053 0.7010 0.0168 0.0001 0.0083 0.5543 0.0021 0.2744 0.0440 0.0002 0 .0218

2548 308 1.3839 0.0045 0.6926 0.0137 0.0000 0.0069 0.4858 0.0016 0.2431 0.0664 0.0002 0.0332

2550 293 1.3361 0.0046 0.7538 0.0180 0.0001 0.0102 0.6464 0.0022 0.3647 0.0382 0. 0001 0.0216

2551 327 1.6982 0.0052 0.8629 0.0183 0.0001 0.0093 0.6772 0.0021 0.3441 0.0541 0.0002 0.0275

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE11

- JAN

- 2010 18:09

Hunti ngdon Life Sciences 00

- 4205 PAGE 485

A PPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN OVARY RIGHT

Animal FINAL BODY

------------------------- ------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

3527 303 1.5313 0.0051 0.7847 0.0182 0.0001 0.0093 0.5141 0.0017 0.2635 0.0548 0.0002 0.0281

3 528 355 1.6385 0.0046 0.8163 0.0150 0.0000 0.0075 0.6621 0.0019 0.3299 0.0750 0.0002 0.0374

3529 287 1.3586 0.0047 0.7257 0.0113 0.0000 0.0060 0.5545 0.0019 0.2962 0.0672 0.0002 0.0359

3531 310 1.5106 0.0049 0.8017 0.0131 0.0000 0.0070 0.6387 0.0021 0.3390 0.0587 0.0002 0.0312

3532 297 1.4079 0.0047 0.7394 0.0127 0.0000 0.0067 0.6935 0.0023 0.3642 0.0629 0.0002 0.0330

3533 305 1.3556 0.0044 0.6875 0.0134 0.0000 0.0068 0.5213 0.0017 0.2644 0.0665 0.0002 0.0337

3535 275 1.3202 0.0048 0.7226 0.0165 0.0001 0.0090 0.4794 0.0017 0.2624 0.0621 0.0002 0.0340

3536 304 1.4837 0.0049 0.7325 0.0152 0.0000 0.0075 0.6035 0.0020 0.2980 0.0566 0.0002 0.0279

3537 288 1.4193 0.0049 0.7099 0.0170 0.0001 0.0085 0.5084 0.0018 0.2543 0.0547 0.0002 0.0274

3538 326 1.3836 0.0042 0.7176 0.0148 0.0000 0.0077 0.5582 0.0017 0.2895 0.0553 0.0002 0.0287

3539 343 1.4865 0.0043 0.7508 0.0170 0.0000 0.0086 0.6979 0.0020 0.3525 0.0622 0.0002 0.0314

3540 320 1.5132 0.0047 0.7725 0.0131 0.0000 0.0067 0.6758 0.0021 0.3450 0.0588 0.0002 0.0300

3541 339 1.6717 0.0049 0.8610 0.0161 0.0000 0.0083 0.7774 0.0023 0.4004 0.0446 0.0001 0.0230

3542 316 1.4166 0.0045 0.7315 0.0140 0.0000 0.0072 0.6194 0.0020 0.3198 0.0547 0.0002 0.0282

3543 291 1.4616 0.0050 0.7845 0.0132 0.0000 0.0071 0.6823 0.0023 0.3662 0.0445 0.0002 0.0239

3544 287 1.2889 0.0045 0.7347 0.0108 0.0000 0.0062 0.5717 0.0020 0.3259 0.0555 0.0002 0.0316

3545 336 1.5393 0.0046 0.8009 0.0156 0.0000 0.0081 0.6036 0.0018 0.3141 0.0536 0.0002 0.0279

3546 335 1.6583 0.0050 0.8589 0.0132 0.0000 0.0068 0.6720 0.0020 0.3481 0.0394 0.0001 0.0204

3547 275 1.3278 0.0048 0.7010 0.0134 0.0000 0.0071 0.5253 0.0019 0.2773 0.0542 0.0002 0.0286

3548 319 1.6905 0.0053 0.8770 0.0136 0.0000 0.0071 0.5809 0.0018 0.3014 0.0449 0.0001 0.0233

3549 289 1.4431 0.0050 0.7291 0.0147 0.0001 0.0074 0.6544 0.0023 0.3306 0.0685 0.0002 0.0346

3550 309 1.6271 0.0053 0.8299 0.0131 0.0000 0.0067 0.5301 0.0017 0.2704 0.0445 0.0001 0.0227

3551 318 1.4594 0.0046 0.7699 0.0189 0.0001 0.0100 0.5338 0.0017 0.2816 0.0592 0.0002 0.0312

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 486

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION T OXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

LUNGS PITUITARY SPLEEN OVARY RIGHT

Animal FINAL BODY

------------------------- ------------------------- ---------- --------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Br ain

g g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4526 288 1.4435 0.0050 0.7266 0.0145 0.0001 0.0073 0.5439 0.0019 0.2738 0.0481 0.0002 0.0242

4527 306 1.2743 0.0042 0.6317 0.0119 0.0000 0.0059 0.4832 0.0016 0.2396 0.0490 0.0002 0.0243

4528 308 1.6807 0.0055 0.8276 0.0184 0.0001 0.0091 0.7423 0.0024 0.3655 0.0686 0.0002 0.0338

4529 353 1.8822 0.0053 0.9395 0.0198 0.0001 0.0099 0.6343 0.0018 0.3166 0.0843 0.0002 0.0421

4530 PNMD 307 1.4619 0.0048 0.8047 0.0106 0.0000 0.0058 0.6494 0.0021 0.3574 0.0832 0.0003 0.0458

4531 317 1.4770 0.0047 0.7510 0.0149 0.0000 0.0076 0.9196 0.0029 0.4676 0.0752 0.0002 0.0382

4532 300 1.4534 0.0048 0.7751 0.0140 0.0000 0.0075 0.5873 0.0020 0.3132 0.0486 0.0002 0.0259

4533 303 1.4590 0.0048 0.7611 0.0114 0.0000 0.0059 0.5999 0.0020 0.3129 0.0443 0.0001 0.0231

4534 300 1.6009 0.0053 0.8621 0.0149 0.0000 0.0080 0.6647 0.0022 0.3580 0.0580 0.0002 0.0312

4535 352 1.6058 0.0046 0.7878 0.0221 0.0001 0.0108 0.6429 0.0018 0.3154 0.0466 0.0001 0.0229

4536 345 1.5805 0.0046 0.8081 0.0157 0.0000 0.0080 0.5700 0.0017 0.2914 0.0627 0.0002 0.0321

4537 312 1.3715 0.0044 0.7246 0.0201 0.0001 0.0106 0.5868 0.0019 0.3100 0.0520 0.0002 0.0275

4539 300 1.3932 0.0046 0.7579 0.0137 0.0000 0.0075 0.5452 0.0018 0.2966 0.0389 0.0001 0.0212

4540 359 1.7763 0.0049 0.8611 0.0199 0.0001 0.0096 0.7263 0.0020 0.3521 0.0612 0.0002 0.0297

4541 290 1.3960 0.0048 0.7223 0.0145 0.0001 0.0075 0.5728 0.0020 0.2964 0.0725 0.0003 0.0375

4542 268 1.3239 0.0049 0.7069 0.0102 0.0000 0.0054 0.5176 0.0019 0.2764 0.0416 0.0002 0.0222

4545 293 1.4014 0.0048 0.7597 0.0119 0.0000 0.0065 0.6004 0.0021 0.3255 0.0544 0.0002 0.0295

4546 352 1.6520 0.0047 0.8590 0.0184 0.0001 0.0096 0.6920 0.0020 0.3598 0.0596 0.0002 0.0310

454 7 365 1.5637 0.0043 0.7626 0.0192 0.0001 0.0094 0.6160 0.0017 0.3004 0.0651 0.0002 0.0317

4548 271 1.1615 0.0043 0.6097 0.0135 0.0000 0.0071 0.5294 0.0020 0.2779 0.0624 0.0002 0.0328

4549 280 1.3490 0.0048 0.6824 0.0187 0.0001 0.0095 0.5736 0.0020 0.2902 0.0507 0.0002 0.0256

4550 282 1.4316 0.0051 0.7515 0.0150 0.0001 0.0079 0.5284 0.0019 0.2774 0.0590 0.0002 0.0310

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 487

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

OVARY LEFT OVARIES UTERUS/OVIDUCTS

Animal FINAL BODY

------------------------- ------------------------- ---- ---------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

1526 268 0.0473 0.0002 0.0245 0.0988 0.0004 0.0511 0.5877 0.0022 0.3038

1527 306 0.0390 0.0001 0.0210 0.0734 0.0002 0.0395 0.9044 0.0030 0.4866

1528 269 0.0575 0.0002 0.0292 0.1293 0.0005 0.0656 0.6518 0.0024 0.3309

1529 309 0.0626 0.0002 0.0312 0.1291 0.0004 0.0642 0.7285 0.0024 0.3625

1530 327 0.0509 0.0002 0.0265 0.1028 0.0003 0.0535 0.5036 0.0015 0.2619

15 31 295 0.0378 0.0001 0.0201 0.0985 0.0003 0.0524 0.4778 0.0016 0.2540

1532 316 0.0430 0.0001 0.0228 0.0930 0.0003 0.0493 0.7759 0.0025 0.4116

1533 329 0.0584 0.0002 0.0288 0.1165 0.0004 0.0575 0.5997 0.0018 0.2958

1535 311 0.0450 0.0001 0.0233 0.0960 0.0003 0.0496 1.0705 0.0034 0.5534

1536 331 0.0337 0.0001 0.0167 0.0859 0.0003 0.0426 0.5840 0.0018 0.2898

1537 342 0.0727 0.0002 0.0359 0.1166 0.0003 0.0575 0.4749 0.0014 0.2342

1539 339 0.0708 0.0002 0.0356 0.1513 0.0004 0.0761 0.5855 0.0017 0.2945

1541 335 0.0476 0.0001 0.0234 0.1141 0.0003 0.0560 1.0390 0.0031 0.5102

1542 300 0.0848 0.0003 0.0457 0.1504 0.0005 0.0811 0.8168 0.0027 0.4405

1543 311 0.0627 0.0002 0.0355 0.1443 0.0005 0.0816 0.9533 0.0031 0. 5393

1544 344 0.0439 0.0001 0.0228 0.1020 0.0003 0.0529 0.5109 0.0015 0.2652

1545 284 0.0662 0.0002 0.0338 0.1165 0.0004 0.0595 0.6452 0.0 023 0.3295

1546 304 0.0464 0.0002 0.0233 0.1045 0.0003 0.0524 0.7678 0.0025 0.3853

1547 291 0.0393 0.0001 0.0203 0.0876 0.0003 0.0452 0.5 400 0.0019 0.2789

1550 268 0.0450 0.0002 0.0223 0.0969 0.0004 0.0480 0.5383 0.0020 0.2665

1551 292 0.0570 0.0002 0.0318 0.1030 0.0004 0.057 6 0.9440 0.0032 0.5275

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEI GHT11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 488

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

OVARY LEFT OVARIES UTERUS/OVIDUCTS

Animal FINAL BODY

------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

2526 303 0.0844 0.0003 0.0447 0.1489 0.0005 0.0789 1.2438 0.0041 0.6590

2527 291 0.0559 0.0002 0.0295 0.1258 0.0004 0.0665 0.7070 0.0024 0.3735

2528 330 0.0492 0.0001 0.0249 0.0985 0.0003 0.0499 0.6708 0.0020 0.3396

2529 292 0.0402 0.0001 0.0213 0.0805 0.0003 0.0426 0.4501 0.0015 0.2 381

2530 291 0.0504 0.0002 0.0264 0.1128 0.0004 0.0591 0.7609 0.0026 0.3989

2531 321 0.0538 0.0002 0.0281 0.0974 0.0003 0.0509 0.5662 0.00 18 0.2957

2532 309 0.0399 0.0001 0.0223 0.0940 0.0003 0.0526 0.7779 0.0025 0.4354

2533 366 0.0686 0.0002 0.0334 0.1618 0.0004 0.0788 0.53 92 0.0015 0.2627

2534 326 0.0618 0.0002 0.0329 0.0999 0.0003 0.0532 0.9750 0.0030 0.5193

2535 299 0.0415 0.0001 0.0244 0.0896 0.0003 0.0527 0.6396 0.0021 0.3759

2537 348 0.0452 0.0001 0.0226 0.1070 0.0003 0.0535 0.5791 0.0017 0.2895

2538 343 0.0612 0.0002 0.0298 0.1113 0.0003 0.0542 0.5657 0.0016 0.2753

2539 315 0.0494 0.0002 0.0242 0.0971 0.0003 0.0476 0.5778 0.0018 0.2830

2540 278 0.0617 0.0002 0.0326 0.1024 0.0004 0.0540 0.5409 0.0019 0.2854

2541 310 0.0720 0.0002 0.0374 0.1285 0.0004 0.0668 0.5194 0.0017 0.2698

2542 298 0.0579 0.0002 0.0304 0.1262 0.0004 0.0663 0.8274 0.0028 0.4344

2543 PNMD 322 0.0566 0.0002 0.0299 0.1032 0.0003 0.0546 0.7186 0.0022 0.3799

2545 331 0.0458 0.0001 0.0236 0.1121 0.0003 0.0579 0.9739 0.0029 0.5028

2546 314 0.0551 0.0002 0.0289 0.1112 0.0004 0.0583 0.5610 0.0018 0.2942

2547 265 0.0483 0.0002 0.0239 0.0911 0.0003 0.0451 0.4982 0.0019 0.2466

2548 308 0.0535 0.0002 0.0268 0.1191 0.0004 0.0596 0.7594 0.0025 0.3800

2550 293 0.0656 0.0002 0.0370 0.1022 0.0003 0.0577 0.8173 0.0028 0.4611

2551 327 0.0570 0.0002 0.0290 0.1105 0.0003 0.0561 0.9137 0.0028 0.4643

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE11

- JAN

- 2010 18:09

Huntingdon Life Sciences 00

- 4205 PAGE 489

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

OVARY LEFT OVARIES UTERUS/OVIDUCTS

Animal FINAL BODY

------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weight %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

3527 303 0.0539 0.0002 0.0276 0.1082 0.0004 0.0554 0.6043 0.0020 0.3097

3528 355 0.0658 0.0002 0.0328 0.1390 0.0004 0.0693 0.6980 0.0020 0.3477

3529 287 0.0547 0.0002 0.0292 0.1198 0.0004 0.0640 0.6603 0.0023 0.3527

3531 310 0.0665 0.0002 0.0353 0.1208 0.0004 0.0641 0.7209 0.0023 0.3826

3532 297 0.0518 0.0002 0.0272 0.1124 0.0004 0.0590 0.4865 0.0016 0.2555

3533 305 0.0705 0.0002 0.0358 0.1377 0.0005 0.0698 0.5298 0.0017 0.2687

3535 275 0.0532 0.0002 0.0291 0.1145 0.0004 0.0627 0.5928 0.0022 0.3245

3536 304 0.0581 0.0002 0.0287 0.1143 0.0004 0.0564 0.5523 0.0018 0.2727

3537 288 0.0418 0.0001 0.0209 0.0969 0.0003 0.0485 1.1840 0.0041 0.5922

3538 326 0.0465 0.0001 0.0241 0.1021 0.0003 0.0530 0.5887 0.0018 0.3053

3539 343 0.0466 0.0001 0.0235 0.1145 0.0003 0.0578 0.4864 0.0014 0.2457

3540 320 0.0495 0.0002 0.0253 0.1083 0.0003 0.0553 0.6317 0.0020 0.3225

3541 339 0.0417 0.0001 0.0215 0.0822 0.0002 0.0423 0.4900 0.0014 0.2524

3542 316 0.0610 0.0002 0.0315 0.1156 0.0004 0.0597 0.5714 0.0018 0.2950

3543 291 0.0308 0.0001 0.0165 0.0755 0.0003 0.0405 0.4602 0.0016 0.2470

3544 287 0.0415 0.0001 0.0237 0.0968 0.0003 0.0552 0.5992 0.0021 0.3416

3545 336 0.0400 0.0001 0.0208 0.0945 0.0003 0.0492 0.5435 0.0016 0.2828

3546 335 0.0600 0.0002 0.0311 0.0960 0.0003 0.0497 0.5125 0.0015 0.2654

3547 275 0.0560 0.0002 0.0296 0.1083 0.0004 0.0572 0.4475 0.0016 0.2362

3548 319 0.0557 0.0002 0.0289 0.1174 0.0004 0.0609 0.6893 0.0022 0.3576

3549 289 0.0621 0.0002 0.0314 0.1291 0.0004 0.0652 0.6766 0.0023 0.3418

3550 309 0.0578 0.0002 0.0295 0.1014 0.0003 0.0517 0.5997 0.0019 0.3059

3551 318 0.0580 0.0002 0.0306 0.1168 0.0004 0.0616 0.5997 0.0019 0.3164

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brain = ORGAN WEIGHT/BRAIN WEIGHT11

- JAN

- 2010 18:0 9

Huntingdon Life Sciences 00

- 4205 PAGE 490

APPENDIX X P0 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

ORGAN AND FINAL BODY WEIGHTS

FEMALES GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

OVARY LEFT OVARIES UTERUS/OVIDUCTS

Animal FINAL BODY

------------------------- ------------------------- -------------------------

Number WEIGHT Weight %FBW %Brain Weight %FBW %Brain Weig ht %FBW %Brain

g g g g

-----------------------------------------------------------------------------------------------------------------------------------

4526 288 0.0524 0.0002 0.0264 0.0995 0.0003 0.0501 0.5068 0.0018 0.2551

4527 306 0.0406 0.0001 0.0201 0.0898 0.0003 0.0445 0.7180 0.0023 0.3560

4528 308 0.0675 0.0002 0.0332 0.1355 0.0004 0.0667 0.6311 0.0021 0.3108

4529 353 0.0580 0.0002 0.0290 0.1414 0.0004 0.0706 1.0146 0.0029 0.5064

4530 PNMD 307 0.0583 0.0002 0.0321 0.1423 0.0005 0.0783 0.4704 0.0015 0.2589

4531 317 0.0524 0.0002 0.0266 0.1259 0.0004 0.0640 0.6767 0.0021 0.3441

4532 300 0.0475 0.0002 0.0253 0.0961 0.0003 0.0513 0.4131 0.0014 0.2203

4533 303 0.0451 0.0001 0.0235 0.0909 0.0003 0.0474 0.4052 0.0013 0.2114

4534 300 0.0609 0.0002 0.0328 0.1178 0.0004 0.0634 1.0442 0.0035 0.5623

4535 352 0.0661 0.0002 0.0324 0.1080 0.0003 0.0530 0.6108 0.0017 0.2996

4536 345 0.0577 0.0002 0.0295 0.1199 0.0003 0.0613 0.5907 0.0017 0.3020

4537 312 0.0451 0.0001 0.0238 0.0979 0.0003 0.0517 0.9512 0 .0030 0.5026

4539 300 0.0489 0.0002 0.0266 0.0848 0.0003 0.0461 0.6156 0.0020 0.3349

4540 359 0.0755 0.0002 0.0366 0.1344 0.0004 0.0652 0 .5192 0.0014 0.2517

4541 290 0.0754 0.0003 0.0390 0.1476 0.0005 0.0764 0.6463 0.0022 0.3344

4542 268 0.0554 0.0002 0.0296 0.0969 0.0004 0.0 517 0.4756 0.0018 0.2540

4545 293 0.0725 0.0002 0.0393 0.1274 0.0004 0.0691 0.7212 0.0025 0.3910

4546 352 0.0586 0.0002 0.0305 0.1145 0.0003 0.0595 0.9667 0.0027 0.5027

4547 365 0.0686 0.0002 0.0335 0.1333 0.0004 0.0650 0.9168 0.0025 0.4471

4548 271 0.0679 0.0003 0.0356 0.1302 0.0005 0.0683 0.7685 0.0028 0.4034

4549 280 0.0535 0.0002 0.0271 0.1073 0.0004 0.0543 0.6419 0.0023 0.3247

4550 282 0.0547 0.0002 0.0287 0.1137 0.0004 0.0597 0.4997 0.0018 0.2623

-----------------------------------------------------------------------------------------------------------------------------------

%FBW = ORGAN WEIGHT/BODY WEIGHT %Brai n = ORGAN WEIGHT/BRAIN WEIGHT

PNMD=PREGNANT, NO MATING DATE

# Huntingdon Life Sciences 00

- 4205 Page 491

211

- DIPE

- 1G Final Report

Individual Pup Organ Weights

Preface Appendix Y

Table of Contents

F 1 

# Generation

Absolute Organ Weights and % Organ to Body Weight Ratios

........................ 492

Absolute Organ Weights and % Organ to Brain Weight Ratios

........................ 500

Key to Abbreviations:

g = Grams11

- JAN

- 2010 18:16

Huntingdon Life Sciences 00

- 4205 PAGE 492

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS ( GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

1526 4 M 87.3 1.6635 1.9055 0.2952 0.3381 0.4230 0.4845

10 F 75.1 1.5478 2.0610 0.2587 0.3445 0.3330 0.4434

1527 8 M 86.4 1.7058 1.9743 0.3929 0.4547 0.3893 0.4506

11 F 80.1 1.5981 1.9951 0.3573 0.4461 0.4231 0.5282

1528 5 M 91.6 1.6295 1.778 9 0.4421 0.4826 0.4600 0.5022

9 F 87.4 1.5541 1.7781 0.3883 0.4443 0.4554 0.5211

1529 2 M 87.0 1.5788 1.8147 0.3681 0.4231 0.2972 0.3416

10 F 79.1 1.4446 1.8263 0.3129 0.3956 0.3297 0.4168

1530 2 M 105.0 1.7142 1.6326 0.5212 0.4964 0.3831 0.3649

5 F 89.9 1.5861 1.7643 0.3204 0.3564 0.3807 0.4235

1531 1 M 64.0 1.5543 2.4286 0.2186 0.3416 0.2291 0.3580

10 F 59.1 1.5119 2.5582 0.2409 0.4076 0.2636 0.4460

1532 9 M 80.7 1.5049 1.8648 0.3813 0.4725 0.2565 0.3178

11 F 71.4 1.4989 2.0993 0.3181 0.4455 0.2338 0.3275

1533 3 M 83.8 1.5961 1.9047 0.3228 0.3852 0.4841 0.5777

13 F 79.5 1.6198 2.0375 0.3349 0.4213 0.3759 0.4728

1535 1 M 59.7 1.5108 2.5307 0.2321 0.3888 0.2323 0.3891

9 F 66.2 1.4593 2.2044 0.3095 0.4675 0.4214 0.6366

1536 4 M 83.3 1.6626 1.9959 0.3779 0.4537 0.3783 0.4541

8 F 82.7 1.5887 1.9210 0.3136 0.3792 0.3615 0.4371

1537 6 M 82.2 1.6615 2.0213 0.3632 0.4418 0.2512 0.3056

13 F 84.9 1.5728 1.8525 0.3423 0.4032 0.3648 0.4297

1539 7 M 88.1 1.6265 1.8462 0.4056 0.4604 0.4740 0.538011

- JAN

- 2010 18:16

Huntingdon Life Sciences 00

- 4205 PAGE 493

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY W EIGHT RATIOS (%)

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight R atio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

1539 (continued)

10 F 69.9 1.4850 2.1245 0.3068 0.4389 0.3819 0.5464

1541 7 M 87.6 1.6447 1.8775 0.3679 0.4200 0.3294 0.3760

12 F 79.4 1.5057 1.8963 0.2854 0.3594 0.3091 0.3893

1542 5 M 76.4 1.5558 2.0364 0.3315 0.4339 0.4013 0.5253

8 F 69.0 1.4586 2.1139 0.2665 0.3862 0.3418 0.4954

1543 6 M 78.6 1.5333 1.9508 0.3536 0.4499 0.2764 0.3517

8 F 77.1 1.5543 2.0160 0.4021 0.5215 0.3261 0.4230

1544 8 M 82.1 1.6006 1.9496 0.3313 0.4035 0.3084 0.3756

13 F 79.6 1.5836 1.9894 0.3876 0.4869 0.3634 0.4565

1545 2 M 66.7 1.4035 2.1042 0.2651 0.3975 0.2398 0.3595

11 F 70.9 1.4983 2.1133 0.3229 0.4554 0.2964 0.4181

1546 5 M 86.2 1.5629 1.8131 0.2981 0.3458 0.2779 0.3224

6 F 75.9 1.5404 2.0295 0.2415 0.3182 0.3008 0.3963

1547 4 M 79.1 1.5266 1.9300 0.3372 0.4263 0.3611 0.4565

6 F 79.5 1.5151 1.9058 0.3277 0.4122 0.3726 0.4687

1550 4 M 72.0 1.4867 2.0649 0.3018 0.4192 0.3157 0.4385

12 F 62.2 1.4719 2.3664 0.2059 0.3310 0.1574 0.2531

1551 2 M 80.7 1.5679 1.9429 0.3678 0.4558 0.3263 0.4043

6 F 65.6 1.4553 2.2184 0.2030 0.3095 0.3242 0.494211

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- 4205 PAGE 494

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

2526 11 M 78.3 1.6181 2.0665 0.2698 0.3446 0.4092 0.5226

16 F 69.3 1.5905 2.2951 0.2807 0.4051 0.3168 0.4571

2527 1 M 76.3 1.5988 2.0954 0.2659 0.3485 0.3047 0.3993

11 F 64.4 1.4222 2.2084 0.2500 0.3882 0.2632 0.4087

2528 3 M 74.2 1.5527 2.0926 0.3482 0.4693 0.3266 0.4402

14 F 66.2 1.4322 2.1634 0.2902 0.4384 0.3215 0.4856

2529 2 M 69.1 1.5433 2.2334 0.2654 0.3841 0.3642 0.5271

12 F 69.5 1.5262 2.1960 0.2988 0.4299 0.3287 0.4729

2530 6 M 95.6 1.6540 1.7301 0.3057 0.3198 0.3476 0.3636

12 F 82.6 1.5712 1.9022 0.3206 0.3881 0.4472 0.5414

2531 3 M 78.0 1.5916 2.0405 0.2910 0.3731 0.3806 0.4879

15 F 77.9 1.4933 1.9169 0.2941 0.3775 0.4895 0.6284

2532 9 M 79.7 1.5641 1.9625 0.3004 0.3769 0.3436 0.4311

13 F 75.2 1.5630 2.0785 0.2834 0.3769 0.3682 0.4896

2533 1 M 91.2 1.6443 1.8 030 0.4076 0.4469 0.5279 0.5788

15 F 82.8 1.6163 1.9521 0.3943 0.4762 0.4913 0.5934

2534 4 M 75.8 1.6325 2.1537 0.3038 0.4008 0.4184 0.5520

11 F 72.2 1.4984 2.0753 0.2873 0.3979 0.4009 0.5553

2535 2 M 69.8 1.3928 1.9954 0.2751 0.3941 0.3546 0.5080

9 F 80.6 1.5202 1.8861 0.2767 0.3433 0.4022 0.4990

2537 3 M 105.5 1.6101 1.5262 0.3987 0.3779 0.5236 0.4963

4 F 88.9 1.5389 1.7310 0.3339 0.3756 0.4197 0.4721

25 38 5 M 89.7 1.6768 1.8693 0.4748 0.5293 0.4829 0.538411

- JAN

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APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP II 2000 MG/M3

---------------------------------------------------------------------------------------------------------------------- -------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

---------------------------------------------------------------------------------------------------------------------------------- -

2538 (continued)

10 F 77.4 1.5574 2.0121 0.3022 0.3904 0.4356 0.5628

2539 4 M 81.1 1.6087 1.9836 0.3032 0.3739 0.3365 0.4149

9 F 80.0 1.5591 1.9489 0.2889 0.3611 0.3161 0.3951

2540 6 M 80.4 1.5624 1.9433 0.3142 0.3908 0.3355 0.4173

7 F 80.2 1.5003 1.8707 0.3046 0.3798 0.3355 0.4183

2541 1 M 79.5 1.5409 1.9382 0.2880 0.3623 0.3293 0.4142

6 F 69.0 1.5358 2.2258 0.2679 0.3883 0.3044 0.4412

2542 5 M 77.2 1.4912 1.9316 0.3327 0.4310 0.3687 0.4776

8 F 77.3 1.4064 1.8194 0.2887 0.3735 0.3306 0.4277

2543 6 M 82.1 1.5275 1.8605 0.3209 0.3909 0.3853 0.4693

12 F 83.1 1.6215 1.9513 0.2981 0.3587 0.5042 0.6067

2545 1 M 73.1 1.5836 2.1663 0.3058 0.4183 0.2838 0.3882

12 F 72.8 1.5095 2.0735 0.2641 0.3628 0.3369 0.4628

2546 1 M 96.5 1.6034 1.6616 0.4144 0.4294 0.4221 0.4374

10 F 90.7 1.5840 1.746 4 0.3410 0.3760 0.4184 0.4613

2547 4 M 71.6 1.6636 2.3235 0.2774 0.3874 0.2726 0.3807

9 F 64.6 1.5846 2.4529 0.2331 0.3608 0.2784 0.4310

2548 1 M 84.4 1.5975 1.8928 0.3106 0.3680 0.3081 0.3650

13 F 74.3 1.5052 2.0258 0.2803 0.3773 0.2923 0.3934

2550 2 M 42.3 1.2478 2.9499 0.2208 0.5220 0.2241 0.5298

8 F 70.6 1.5451 2.1885 0.3113 0.4409 0.3797 0.5378

2551 2 M 90.2 1.5684 1.7388 0.4391 0.4868 0.4607 0.5108

10 F 84.5 1.5600 1.8462 0.3733 0.4418 0.4498 0.532311

- JAN

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- 4205 PAGE 496

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP III 10000 MG/M3

------------------------------------------------------------------------------------------------------------------------ -----------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

3527 3 M 74.4 1.4606 1.9632 0.2870 0.3858 0.2996 0.4027

13 F 69.4 1.5148 2.1827 0.2561 0.3690 0.3588 0.5170

3528 2 M 77.2 1.5610 2.0220 0.3620 0.4689 0.3477 0.4504

15 F 82.7 1.6389 1.9817 0.3325 0.4021 0.4825 0.5834

3529 5 M 80.8 1.5563 1.9261 0.3459 0.4281 0.4578 0.5666

14 F 72.2 1.5221 2.1082 0.2726 0.3776 0.3983 0.5517

3531 5 M 41.9 1.4318 3.4172 0.1480 0.3532 0.1180 0.2816

9 F 65.6 1.4859 2.2651 0.2530 0.3857 0.3048 0.4646

3532 9 F 83.8 1.6259 1.9402 0.3346 0.3993 0.4296 0.5126

12 M 81.3 1.6251 1.9989 0.3272 0.4025 0.4481 0.5512

3533 1 M 68.1 1.5061 2.2116 0.2652 0.3894 0.2893 0.4248

13 F 60.3 1.4384 2.3854 0.1954 0.3240 0.3448 0.5718

3535 1 M 60.4 1.4675 2.4296 0.2392 0.3960 0.2253 0.3730

15 F 55.9 1.4045 2.5125 0.1782 0.3188 0.2591 0.4635

3536 2 M 77.3 1.6217 2.0979 0.3359 0.4345 0.4429 0.5730

10 F 70.5 1.5073 2.1380 0.2840 0.4028 0.3768 0.5345

3537 3 M 86.3 1.6212 1.8786 0.3290 0.3812 0.4376 0.5071

13 F 75.8 1.5736 2.0760 0.2926 0.3860 0.3384 0.4464

3538 1 M 77.5 1.6451 2.1227 0.2985 0.3852 0.3570 0.4606

11 F 77.5 1.5596 2.0124 0.3369 0.4347 0.3746 0.4834

3539 5 M 85.2 1.6564 1 .9441 0.4276 0.5019 0.3941 0.4626

11 F 67.4 1.5593 2.3135 0.2644 0.3923 0.2913 0.4322

3540 2 M 85.6 1.5612 1.8238 0.3886 0.4540 0.3800 0.443911

- JAN

- 2010 18:16

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- 4205 PAGE 497

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BO DY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

3540 (continued)

13 F 80.2 1.5592 1.9441 0.3123 0.3894 0.4793 0.5976

3541 5 M 98.4 1.6801 1.7074 0.4746 0.4823 0.4214 0.4283

14 F 89.8 1.5283 1.7019 0.3730 0.4154 0.4039 0.4498

3542 1 M 87.2 1.7123 1.9636 0.4146 0.4755 0.4047 0.4641

8 F 80.4 1.6336 2.0318 0.2808 0.3493 0.4060 0.5050

3543 3 M 71.8 1.4558 2.0276 0.2913 0.4057 0.3286 0.4577

9 F 71.7 1.4384 2.0061 0.2647 0.3692 0.3486 0.4862

3544 2 M 70.2 1.4253 2.0303 0.4062 0.5786 0.3154 0.4493

10 F 68.5 1.3822 2.0178 0.3317 0.4842 0.3384 0.4940

3545 3 M 91.3 1.6104 1.7639 0.3438 0.3766 0.3912 0.4285

13 F 78.4 1.5311 1.9529 0.2695 0.3437 0.3564 0.4546

3546 6 M 89.9 1.6226 1.8049 0.4419 0.4915 0.3793 0.4219

10 F 73.9 1.5126 2.0468 0.3273 0.4429 0.3550 0.4804

3547 6 M 78.4 1.4517 1.8517 0.3239 0.4131 0.3827 0.4881

7 F 67.5 1.3869 2.0547 0.3343 0.4953 0.3582 0.5307

3548 2 M 92.3 1.5685 1.6993 0.3662 0.3967 0.5282 0.5723

9 F 77.2 1.5315 1.9838 0.2989 0.3872 0.4246 0.5500

3549 2 M 80.8 1.6042 1.9854 0.4282 0.5300 0.4164 0.5153

8 F 73.9 1.5253 2.0640 0.3449 0.4667 0.4253 0.5755

3550 7 M 63.8 1.5579 2.4418 0.2227 0.3491 0.2234 0.3502

13 F 69.6 1.5527 2.2 309 0.3180 0.4569 0.3543 0.5091

3551 5 M 88.6 1.6458 1.8576 0.3949 0.4457 0.4535 0.5119

14 F 76.1 1.4841 1.9502 0.2593 0.3407 0.3728 0.489911

- JAN

- 2010 18:16

Huntingdon Life Sciences 00

- 4205 PAGE 498

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP IV 20000 MG /M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

4526 6 M 69.4 1.5900 2.2911 0.2610 0.3761 0.3437 0.4952

13 F 65.9 1.5093 2.2903 0.2536 0.3848 0.2959 0.4490

4527 2 M 82.3 1.6845 2.0468 0.2989 0.3632 0.3947 0.4796

10 F 72.9 1.5719 2.1562 0.2577 0.3535 0.3715 0.5096

4528 5 M 73.3 1.5686 2.1400 0.3223 0.4397 0.3162 0.4314

11 F 68.6 1.4889 2.1704 0.2670 0.3892 0.3539 0.5159

4529 3 M 77.1 1.6241 2.1065 0.3153 0.4089 0.2591 0.3361

15 F 77.3 1.5165 1.9618 0.2907 0.3761 0.3411 0.4413

4530 3 M 73.3 1.5800 2.1555 0.2314 0.3157 0.2733 0.3729

7 F 73.0 1.4575 1.9966 0.2425 0.3322 0.3562 0.4879

4531 3 M 71.9 1.5429 2.1459 0.3105 0.4318 0.3014 0.4192

9 F 71.3 1.4720 2.0645 0.3094 0.4339 0.3167 0.4442

4532 8 M 92.5 1.6124 1.7431 0.4105 0.4438 0.4977 0.5381

11 F 74.1 1.5440 2.0837 0.3080 0.4157 0.3749 0.5059

4533 6 M 64.3 1.4033 2.1824 0.3446 0.5359 0.3006 0.4675

9 F 69.2 1.4734 2.1292 0.3914 0.5656 0.2759 0.3987

4534 4 M 85.4 1.6063 1.8809 0.3910 0.4578 0.3234 0.3787

12 F 80.4 1.5083 1.8760 0.2976 0.3701 0.3687 0.4586

4535 6 M 74.0 1.5830 2.1392 0.2355 0.3182 0.3228 0.4362

15 F 74.5 1.5242 2.0459 0.2357 0.3164 0.3881 0.5209

4536 6 M 89.5 1.6077 1.7963 0.3940 0.4402 0.4485 0.5011

9 F 73.5 1.5048 2.0473 0.2562 0.3486 0.3505 0.4769

4537 2 M 70.6 1.5821 2.2409 0.2514 0.3561 0.2224 0.315011

- JAN

- 2010 18:16

Huntingdon Life Sciences 00

- 4205 PAGE 499

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHTS (GRAMS) AND ORGAN/BODY WEIGHT RATIOS (%)

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

4537 (continued)

9 F 83.9 1.5349 1.8294 0.3574 0.4260 0.3401 0.4054

4539 3 M 84.0 1.6141 1.9215 0.2813 0.3349 0.2915 0.3470

8 F 84.2 1.5236 1.8095 0.3069 0.3645 0.4142 0.4919

4540 6 M 88.1 1.5921 1.8072 0.3782 0.4293 0.4326 0.4910

12 F 77.2 1.5358 1.9894 0.3555 0.4605 0.4391 0.5688

45 41 3 M 70.7 1.5188 2.1482 0.3050 0.4314 0.2143 0.3031

12 F 79.4 1.4771 1.8603 0.3549 0.4470 0.3694 0.4652

4542 2 M 92.7 1.5190 1.6386 0.3666 0.3955 0.5598 0.6039

7 F 80.0 1.5489 1.9361 0.2422 0.3027 0.4369 0.5461

4545 6 M 75.5 1.5021 1.9895 0.3198 0.4236 0.4134 0.5475

11 F 63.6 1.4356 2.2572 0.2223 0.3495 0.3621 0.5693

4546 4 M 72.3 1.5617 2.1600 0.3026 0.4185 0.3637 0.5030

10 F 70.2 1.4631 2.0842 0.2413 0.3437 0.3731 0.5315

4547 2 M 88.1 1.5124 1.7167 0.3743 0.4249 0.3504 0.3977

13 F 76.7 1.5273 1.9913 0.2870 0.3742 0.3658 0.4769

4548 1 M 58.6 1.4496 2.4737 0.2164 0.3693 0.2596 0.4430

12 F 65.7 1.5342 2.3352 0.2643 0.4023 0.3383 0.5149

4549 6 M 78.4 1.5552 1.9837 0.2918 0.3722 0.3282 0.4186

10 F 73.6 1.5510 2.1073 0.2864 0.3891 0.2909 0.3952

4550 8 M 69.9 1.5266 2.1840 0.2512 0.3594 0.3516 0.5030

12 F 75.1 1.5166 2.0194 0.2872 0.3824 0.35 65 0 .474711

- JAN

- 2010 18:17

Huntingdon Life Sciences 00

- 4205 PAGE 500

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

1526 4 M 1.6635 0.2952 17.746 0.4230 25.428

10 F 1.5478 0.2587 16.714 0.3330 21.514

1527 8 M 1.7058 0.3929 23.033 0.3893 22.822

11 F 1.5981 0.3573 22.358 0.4231 26.475

1528 5 M 1.6295 0.4421 27.131 0.4600 28.230

9 F 1.5541 0.3883 2 4.986 0.4554 29.303

1529 2 M 1.5788 0.3681 23.315 0.2972 18.824

10 F 1.4446 0.3129 21.660 0.3297 22.823

1530 2 M 1.7142 0.5212 30.405 0.3831 22.349

5 F 1.5861 0.3204 20.200 0.3807 24.002

1531 1 M 1.5543 0.2186 14.064 0.2291 14.740

10 F 1.5119 0.2409 15.934 0.2636 17.435

1532 9 M 1.5049 0.3813 25.337 0.2565 17.044

11 F 1.4989 0.3181 21.222 0.2338 15.598

1533 3 M 1.5961 0.3228 20.224 0.4841 30.330

13 F 1.6198 0.3349 20.675 0.3759 23.207

1535 1 M 1.5108 0.2321 15.363 0.2323 15.376

9 F 1.4593 0.3095 21.209 0.4214 28.877

1536 4 M 1.6626 0.3779 22.729 0.3783 22.754

8 F 1.5887 0.3136 19.739 0.3615 22.754

1537 6 M 1.6615 0.3632 21.860 0.2512 15.119

13 F 1.5728 0.3423 21.764 0.3648 23.194

1539 7 M 1.6265 0.4056 24.937 0.4740 29.14211

- JAN

- 2010 18:17

Huntingdon Life Sciences 00

- 4205 PAGE 501

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION R EPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP I 0 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYM US

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

1539 (continued)

10 F 1.4850 0.3068 20.660 0.3819 25.717

1541 7 M 1.6447 0.3679 22.369 0.3294 20.028

12 F 1.5057 0.2854 18.955 0.3091 20.529

1542 5 M 1.5558 0.3315 21.307 0.4 013 25.794

8 F 1.4586 0.2665 18.271 0.3418 23.433

1543 6 M 1.5333 0.3536 23.061 0.2764 18.026

8 F 1.5543 0.4021 25.870 0.3261 20.981

1544 8 M 1.6006 0 .3313 20.698 0.3084 19.268

13 F 1.5836 0.3876 24.476 0.3634 22.948

1545 2 M 1.4035 0.2651 18.888 0.2398 17.086

11 F 1.4983 0.3229 21.551 0.2964 19.782

1546 5 M 1.5629 0.2981 19.074 0.2779 17.781

6 F 1.5404 0.2415 15.678 0.3008 19.527

1 547 4 M 1.5266 0.3372 22.088 0.3611 23.654

6 F 1.5151 0.3277 21.629 0.3726 24.592

1550 4 M 1.4867 0.3018 20.300 0.3157 21.235

12 F 1.4719 0.2059 13.989 0.1574 10.694

1551 2 M 1.5679 0.3678 23.458 0.3263 20.811

6 F 1.4553 0.2030 13.949 0.3242 22.27711

- JAN

- 2010 18:17

Huntingdon Life Sciences 00

- 4205 PAGE 502

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pu p Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-------------------------------------------------------------------------------------------------------------- ---------------------

2526 11 M 1.6181 0.2698 16.674 0.4092 25.289

16 F 1.5905 0.2807 17.649 0.3168 19.918

2527 1 M 1.5988 0.2659 16.631 0.3047 19.058

11 F 1.4222 0.2500 17.578 0.2632 18.507

2528 3 M 1.5527 0.3482 22.425 0.3266 21.034

14 F 1.4322 0.2902 20.263 0.3215 22.448

2529 2 M 1.5433 0.2654 17.197 0.3642 23.599

12 F 1.5262 0.2988 19.578 0.3287 21.537

2530 6 M 1.6540 0.3057 18.482 0.3476 21.016

12 F 1.5712 0.3206 20.405 0.4472 28.462

2531 3 M 1.5916 0.2910 18.283 0.3806 23.913

15 F 1.4933 0.2941 19.695 0.4895 32.780

2532 9 M 1.5641 0.3004 19.206 0.3436 21.968

13 F 1.5630 0.2834 18.132 0.3682 23.557

2533 1 M 1.6443 0.4076 24.789 0.5279 32.105

15 F 1.6163 0.3943 24.395 0.4913 30.397

2534 4 M 1.6325 0.3038 18.609 0.4184 25.629

11 F 1.4984 0.2873 19.174 0.4009 26.755

2535 2 M 1.3928 0.2751 19.752 0.3546 25.460

9 F 1.5202 0.2767 18.202 0.4022 26.457

2537 3 M 1.6101 0.3987 24.762 0.5236 32.520

4 F 1.5389 0.3339 21.697 0.4197 27.273

2538 5 M 1.6768 0.4748 28.316 0.4829 28.79911

- JAN

- 2010 18:17

Huntingdon Life Sciences 00

- 4205 PAGE 503

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP II 2000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

2538 (continued)

10 F 1.5574 0.3022 19.404 0.4356 27.970

2539 4 M 1.6087 0.3032 18.848 0.3365 20.918

9 F 1.5591 0.2889 18.530 0.3161 20.275

2540 6 M 1.5624 0.3142 20.110 0.3355 21.473

7 F 1.5003 0.3046 20.303 0.3355 22.362

2541 1 M 1.5409 0.2880 18.690 0.3293 21.371

6 F 1.5358 0.2679 17.444 0.3044 19.820

2542 5 M 1.4912 0.3327 22.311 0.3687 24.725

8 F 1.4064 0.2887 20.528 0.3306 23.507

2543 6 M 1.5275 0.3209 21.008 0.3853 25.224

12 F 1.6215 0.2981 18.384 0.5042 31.095

2545 1 M 1.5836 0.3058 19.310 0.2838 17.921

12 F 1.5095 0.2641 17.496 0.3369 22.319

2546 1 M 1.6034 0.4144 25.845 0 .4221 26.325

10 F 1.5840 0.3410 21.528 0.4184 26.414

2547 4 M 1.6636 0.2774 16.675 0.2726 16.386

9 F 1.5846 0.2331 14.710 0.2784 17.569

2548 1 M 1.5975 0.3106 19.443 0.3081 19.286

13 F 1.5052 0.2803 18.622 0.2923 19.419

2550 2 M 1.2478 0.2208 17.695 0.2241 17.960

8 F 1.5451 0.3113 20.148 0.3797 24.574

2551 2 M 1.5684 0.4391 27.997 0.4607 29.374

10 F 1.5600 0.3733 23.929 0.4498 28.83311

- JAN

- 2010 18:17

Huntingdon Life Sciences 00

- 4205 PAGE 504

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

3527 3 M 1.4606 0.2870 19.649 0.2996 20.512

13 F 1.5148 0.2561 16.907 0.3588 23.686

3528 2 M 1.5610 0.3620 23.190 0.3477 22.274

15 F 1.6389 0.3325 20.288 0.4825 29.440

3529 5 M 1.5563 0.3459 22.226 0.4578 29.416

14 F 1.5221 0.2726 17.909 0.3983 26.168

3531 5 M 1.4318 0.1480 10.337 0.1180 8.241

9 F 1.4859 0.2530 17.027 0.3048 20.513

3532 9 F 1.6259 0.3346 20.579 0.4296 26.422

12 M 1.6251 0.3272 20.134 0.4481 27.574

3533 1 M 1.5061 0.2652 17.608 0.2893 19.209

13 F 1.4384 0.1954 13.585 0.3448 23.971

3535 1 M 1.4675 0.2392 16.300 0.2253 15.353

15 F 1.4045 0.1782 12.688 0.2591 18.448

3536 2 M 1.6217 0.3359 20.713 0.4429 27.311

10 F 1.5073 0.2840 18.842 0.3768 24.998

3537 3 M 1.6212 0.3290 20.294 0.4376 26.992

13 F 1.5736 0.2926 18.594 0.3384 21.505

3538 1 M 1.6451 0.2985 18.145 0.3570 21.701

11 F 1.5596 0.3369 21.602 0.3746 24.019

3539 5 M 1.6564 0.4276 25.815 0.3941 23.793

11 F 1.5593 0.264 4 16.956 0.2913 18.681

3540 2 M 1.5612 0.3886 24.891 0.3800 24.34011

- JAN

- 2010 18:17

Hun tingdon Life Sciences 00

- 4205 PAGE 505

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP III 10000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- ------------- - --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

3540 (continued)

13 F 1.5592 0.3123 20.030 0.4793 30.740

3541 5 M 1.6801 0.4746 28.248 0.4214 25.082

14 F 1.5283 0.3730 24.406 0.4039 26.428

3542 1 M 1.7123 0.4146 24.213 0.4047 23.635

8 F 1.6336 0.2808 17.189 0.4060 24.853

3543 3 M 1.4558 0.2913 20.010 0.3286 22.572

9 F 1.4384 0.2647 18.402 0.3486 24.235

3544 2 M 1.4253 0.4062 28.499 0.3154 22.129

10 F 1.3822 0.3317 23.998 0.3384 24.483

3545 3 M 1.6104 0.3438 21.349 0.3912 24.292

13 F 1.5311 0.2695 17.602 0.3564 23.277

3546 6 M 1.6226 0.4419 27.234 0.3793 23.376

10 F 1.5126 0.3273 21.638 0.3550 23.470

3547 6 M 1.4517 0.3239 22.312 0.3827 26.362

7 F 1.3869 0.3343 24.104 0.3582 25.827

3548 2 M 1.5685 0.3662 23.347 0.5282 33.675

9 F 1.5315 0.2989 19.517 0.4246 27.724

3549 2 M 1.6042 0.4282 26.692 0.4164 25.957

8 F 1.5253 0.3449 22.612 0.4253 27.883

3550 7 M 1.5579 0.2227 14.295 0.2234 14.340

13 F 1.5527 0.3180 20.480 0.3543 22.818

3551 5 M 1.6458 0.3949 23.994 0.4535 27.555

14 F 1.4841 0.2593 17.472 0.3728 25.12011

- JAN

- 2010 18:17

Hunti ngdon Life Sciences 00

- 4205 PAGE 506

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Number No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

4526 6 M 1.5900 0.2610 16.415 0.3437 21.616

13 F 1.5093 0.2536 16.802 0.2959 19.605

4527 2 M 1.6845 0.2989 17.744 0.3947 23.431

10 F 1.5719 0.2577 16.394 0.3715 23.634

4528 5 M 1.5686 0.3223 20.547 0.3162 20.158

11 F 1.4889 0.2670 17.933 0.3539 23.769

4529 3 M 1.6241 0.3153 19.414 0.2591 15.953

15 F 1.5165 0.2907 19.169 0.3411 22.493

4530 3 M 1.5800 0.2314 14.646 0.2733 17.297

7 F 1.4575 0.2425 16.638 0.3562 24.439

4531 3 M 1.5429 0.3105 20.124 0.3014 19.535

9 F 1.4720 0.3094 21.019 0.3167 21.515

4532 8 M 1.6124 0.4105 25.459 0.4977 30.867

11 F 1.5440 0.3080 19.948 0.3749 24.281

4533 6 M 1.4033 0.3446 24.556 0.3006 21.421

9 F 1.4734 0.3914 26.564 0.2759 18.725

4534 4 M 1.6063 0.3910 24.342 0.3234 20.133

12 F 1.5083 0.2976 19.731 0.3687 24.445

4535 6 M 1.5830 0.2355 14.877 0.3228 20.392

15 F 1.5242 0.2357 15.464 0.3881 25.463

4536 6 M 1.6077 0.3940 24.507 0.4485 27.897

9 F 1.5048 0.2562 17.026 0.3505 23.292

4537 2 M 1.5821 0.2514 15.890 0.2224 14.05711

- JAN

- 2010 18:17

Huntingdon Life Sciences 00

- 4205 PAGE 507

APPENDIX Y F1 GENERATION

GASOLINE DIPE VAPOR CONDENSATE: A ONE

- GENERATION WHOLE

- BODY

INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL PUP ORGAN WEIGHT (GRAMS) AND ORGAN/BRAIN WEIGHT RATIOS (%) .

GROUP IV 20000 MG/M3

-----------------------------------------------------------------------------------------------------------------------------------

Final BRAIN SPLEEN THYMUS

Animal Pup Body

-------------- -------------- --------------

Numb er No. Sex Weight Weight Ratio Weight Ratio Weight Ratio

-----------------------------------------------------------------------------------------------------------------------------------

4537 (continued)

9 F 1.5349 0.3574 23.285 0.3401 22.158

4539 3 M 1.6141 0.2813 17.428 0.2915 18.060

8 F 1.5236 0.3069 20.143 0.4142 27.186

4540 6 M 1.5921 0.3782 23.755 0.4326 27.172

12 F 1.5358 0.3555 23.148 0.4391 28.591

4541 3 M 1.5188 0.3050 20.082 0.2143 14.110

12 F 1.4771 0.3549 24.027 0.3694 25.008

4542 2 M 1.5190 0.3666 24.134 0.5598 36.853

7 F 1.5489 0.2422 15.637 0.4369 28.207

4545 6 M 1.5021 0.3198 21.290 0.4134 27.521

11 F 1.4356 0.2223 15.485 0.3621 25.223

4546 4 M 1.5617 0.3026 19.376 0.3637 23.289

10 F 1.4631 0.2413 16.492 0.3731 25.501

4547 2 M 1.5124 0.3743 24.749 0.3504 23.168

13 F 1.5273 0.2870 18.791 0.3658 23.951

4548 1 M 1.4496 0.2164 14.928 0.2596 17.908

12 F 1.5342 0.2643 17.227 0.3383 22.051

4549 6 M 1.5552 0.2918 18.763 0.3282 21.103

10 F 1.5510 0.2864 18.466 0.2909 18.756

4550 8 M 1.5266 0.2512 16.455 0.3516 23.032

12 F 1.5166 0.2872 18.937 0.3565 23.507

# Huntingdon Life Sciences 00

- 4205 Page 508

211

- DIPE

- 1G Final Report

Individual Animal Gross

and Microscopic Observations

Preface Appendix Z

Key to Abbreviations

Gl = Gland

Oviducts/Fallop = Oviducts/Fallopian

Notes

1. Unless otherwise specified in a histopathology note, the organ/tissue

examined was the required (routine) section.

2. The dimensions of gross lesions are presented as a general range based on

the actual dimensions noted during macroscopic examinations. The actual

dimensions of gross lesions can be found in the raw data.

3. For summarization purposes, descriptive comments [i.e., location of scab(s)

an d/or sore(s), etc.] are not presented in this appendix. These data are

contained in the study raw data if needed.

Corresponding exposure levels for each group were as follows:

Group 1

- 0 mg/m 3



# Group 2

- 2,000 mg/m 3



# Group 3

- 10,000 mg/m 3



# Group 4

- 20 ,000 mg/m 3Page 509Page 510Page 511Page 512Page 513Page 514Page 515Page 516Page 517Page 518Page 519Page 520Page 521Page 522Page 523Page 524Page 525Page 526Page 527Page 528Page 529Page 530Page 531Page 532Page 533Page 534Page 535Page 536Page 537Page 538Page 539Page 540Page 541Page 542Page 543Page 544Page 545Page 546Page 547Page 548Page 549

# AUDITED FINAL REPORT

Study Phase: Sperm Analysis

Test Site Phase Reference Number 93075132

Te s ting Facility Study Number 00-4205

Sponsor Reference Number 211-DIPE-1G



# Gas oline DIPE Vapor Condensate:

A One-Generation Whole-Body Inhalation Reproduction

Toxicity Study in Rats

TEST SITE:

Cha rles River Laboratories, Pathology Associates - Maryland

15 Worman’s Mill Court, Suite I

Frederick, MD 21701

TESTING FACILITY:

Huntington Life Sciences (HLS)

Mettlers Road

P.O. Box 2360

East Millstone, NJ 08875-2360

SPONSOR:

American Petroleum Institute (API)

1220 L Street, Northwest

Washington, D.C. 20005-4070

09-APR-2009

Page 1 of 14 Appendix AA Page 550

## Final Report

HLS Study No.: 00-4205

2 

# TABLE OF CONTENTS

Section Page No.



# I. QUALITY ASSURANCE STATEMENT ............................. 4

II. MATERIALS AND METHODS ................................... 5

A. Sample Collection................................... 5

B. Sperm Motility Evaluation........................... 5

C. Caudal Epididymis Weight and Total Sperm Count

Determination ...................................... 6

D. Sperm Morphology Evaluation......................... 6

E. Statistical Analyses................................ 6

F. GLP Compliance Statement............................ 7

G. Sample and Data Archiving........................... 7

III. RESULTS ................................................. 7

A. Sperm Motility...................................... 7

B. Total Sperm Count................................... 7

C. Sperm Morphology.................................... 8

D. Caudal Epididymis Weight............................ 8

IV. DISCUSSION AND CONCLUSIONS .............................. 8

V. REFERENCES .............................................. 9 Page 551

## Final Report

HLS Study No.: 00-4205

3 

# INDEX OF TABLES AND APPENDICES

Table Page No.



# 1. Summary of Male Reproductive Parameters ................ 10

Appendix Page No.



# A. Individual Caudal Epididymis Weight,

Sperm Motility and Sperm Count Data .................... 11

B. Individual Sperm Morphology Data ........................ 13 Page 552Page 553

## Final Report

HLS Study No.: 00-4205

5 

# Gasoline DIPE Vapor Condensate:

A One-Generation Whole-Body Inhalation Reproduction Toxicity

Study in Rats

II. MATERIALS AND METHODS

A. Sample Collection

All surviving male rats were euthanized by exsanguination

following anesthesia with inhaled carbon dioxide after the last F 1 

# litters were weaned. For all animals, the abdominal cavity was

opened and the reproductive organs exposed. For motility

assessment, the left vas deferens was dissected away from the

testis and immediately placed in a petri dish containing 10 ml of

a solution consisting of 1% Bovine Serum Albumin dissolved in

Phosphate Buffered Saline. The solution was prewarmed to a

temperature of approximately 38 o 

# C. A minimum 3-minute period was

allowed for the sperm to swim out.

For total sperm count assessment, the left epididymis and

left testis were then removed and placed on dry ice. The frozen

epididymides and testes were then transferred to Pathology

Associates, A Division of Charles River Laboratories, Inc.

(formerly known as Pathology Associates International until

January 8, 2001), Frederick, MD. and stored frozen at -70 o 

# C until

evaluation for caudal epididymal sperm count and homogenizationresistant testicular spermatid count.

Only the control and high-exposure animals were examined for

percent motility, total epididymal sperm, homogenization-resistant

testicular spermatid count, sperm morphology and caudal epididymis

weight.

B. Sperm Motility Evaluation



# Following the swim out period, a sperm sample was obtained

using a 100 μ

m deep cannula. The cannula was immediately loaded

into the prewarmed stage of the Hamilton Thorne IVOS (Ver. 12.1c)

automated sperm analyzer. The analyzer automatically selected

five fields and each motion image was digitally saved and

permanently stored on optical media. The images were

subsequently analyzed and the percent motility determined for

each animal. Page 554

## Final Report

HLS Study No.: 00-4205

6 

# C. Caudal Epididymis Weight and Total Sperm Count Determination

Each frozen epididymis was removed from the freezer, thawed

and the caudal section was trimmed and weighed. Each frozen

testis was removed from the freezer, thawed, the tunica removed

and the testicular parenchyma weighed. The cauda epididymis and

testicular parenchyma were homogenized in deionized water and the

suspensions were transferred to plastic test tubes and vortexed. A

100 μ l sample was transferred to a violet reaction vial containing

a Hoechst dye (H33342) which uniquely stains the head of the

sperm. A sample of the stained sperm was placed into a 20 μ m deep

Cell-Vu glass slide which was loaded into the Hamilton Thorne IVOS

(Ver. 12.1c) analyzer. Twenty fields were automatically selected

by the analyzer for each animal and the number of homogenizationresistant spermatids and cauda epididymal sperm determined. The

counts reported were adjusted for caudal epididymal or testicular

weight.

D. Sperm Morphology Evaluation



# Two Eosin stained slides were prepared for each animal from

the caudal epididymis total count preparation. The slides were

evaluated and a minimum of 200 sperm cells/animal was examined for

morphological development.

E. Statistical Analyses

The means and standard deviations for the sperm motility,

caudal epididymal sperm count, testicular homogenization-resistant

spermatid count and sperm morphology data were calculated and

compared across groups using the Kruskal-Wallis nonparametric

ANOVA test. If a significant effect occurred (p<0.05), the MannWhitney U test was used for pair-wise comparisons of each treated

group to the control group. Animals with fewer than 25 sperm

cells present in the motility analysis were excluded from the

group mean calculation and subsequent statistical analysis.

Animals with at least 25 sperm cells were included in the group

mean calculation subsequent and statistical analysis; however,

animals with fewer than 50 cells were identified to have a low

count. The mean caudal epididymis weight for each group was

calculated and compared using one-way analysis of variance (ANOVA)

technique. If ANOVA is significant, Dunnett's test was used for

pair-wise comparisons of each treated group to the control group

at the 5% and 1% risk levels. Statistical analyses were

performed using an IBM TM 

# compatible computer with SAS computer

programs (SAS/STAT User's Guide, 1989). 

# Page 555

## Final Report

HLS Study No.: 00-4205

7 

# F. GLP Compliance Statement

The portion of this study performed by PAI was conducted in

compliance with US EPA 79.60, CFR Vol. 59, No. 122, 27 June 1994.

G. Sample and Data Archiving



# Frozen epididymis and testis samples not processed for sperm

count determination were transferred to and stored frozen at -70 o 

# C

in PAI's long-term archive facility. The optical media used for

permanent storage of the sperm motility images, raw data printouts

from the sperm motility, epididymal and testicular sperm count and

sperm morphology analysis, as well as any supporting documentation

and the sperm morphology slides will be maintained at PAI until

the study has been completed. Upon finalizing the report, all

non-frozen study material will be returned to HLS for archiving.

Disposition of the frozen tissue samples will be directed by

correspondence from HLS.

III. RESULTS

A. Sperm Motility

Table 1 (Summary Data)

Appendix A (Individual Data)

No apparent treatment-related effects were observed in the

sperm motility data. Group mean values were 90% and 93% for the

control and high-exposure groups, respectively. There was one

animal each in the control (animal 1026) and high-exposure (animal

4026) group for which sperm motility images were not recorded due

to a technical problem with the IVOS sperm analyzer. There was

one animal (animal 1048) in the control group with no motile

sperm. Animal 1034 in the control group had fewer than 25 sperm

cells counted and was excluded from the group mean.

B. Total Sperm Count



# Table 1 (Summary Data)

Appendix A (Individual Data)

The number of sperm per gram of caudal epididymis and the

number of spermatids per gram of testis were comparable between

the control and high-exposure groups. Group mean values were 831.6

and 927.9 million sperm/gram for the epididymal sperm count and

101.2 and 103.9 million sperm/gram for the homogenizationresistant testicular count. Page 556Page 557

## Final Report

HLS Study No.: 00-4205

9 

# V. REFERENCES

Dunlap, W.P., Marx, M.S. and Agamy, G.G. 1981. Fortran IV

functions for calculating probabilities associated with

Dunnett’s test. Behav. Res. Methods and Instrumentation 13:

363-366.

Dunnett, C. W. 1955. A multiple comparison procedure for

comparing several treatments with a control. Journal of the

American Statistical Association 50:1096-1121.

Dunnett, C. W. 1964. New tables for multiple comparisons with

a control. Biometrics 20:482-491.

Kruskal, W.H. and Wallis, W.A. 1952. Use of ranks in onecriterion variance analysis. Journal of the American

Statistical Association 47: 583-621.

Kruskal, W.H. and Wallis, W.A. 1953. Errata for KruskalWallis (1952). Journal of the American Statistical

Association 48: 907-911.

Kruskal, W.H. 1957. Historical notes on the Wilcoxon unpaired

two-sample test. Journal of the American Statistical

Association 52: 356-360.

SAS/STAT User's Guide, 1989a. SAS Institute Inc., Version 6,

Fourth Edition, Cary, NC:SAS Institute Inc., 1989. Vol. 1,

p. 209-244. Vol. 2 p. 1195-1210. Page 558

### 10



## HLS STUDY NO.: 00-4205

TABLE 1

GASOLINE DIPE VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS

SUMMARY OF MALE REPRODUCTIVE PARAMETERS

Group: I IV

Exposure Level: GDIPEVC a 

## (mg/m 3 

## ) 0 20,000

MOTILITY (%)

Mean 90 93

SD 20 6

N 24 25

EPIDIDYMAL COUNT

(MILLION SPERM/GRAM)

Mean 831.6 927.9

SD 189.1 177.2

N 26 26

TESTICULAR COUNT

(MILLION SPERM/GRAM)

Mean 101.2 103.9

SD 16.1 12.0

N 26 26

SPERM MORPHOLOGY b



## (% ABNORMAL)

Mean 7.4 0.4 *



## SD 23.5 0.5

N 26 26

CAUDAL EPIDIDYMIS WEIGHTS

(GRAM)

Mean 0.26 0.27 *



## SD 0.03 0.02

N 26 26

a 

## GASOLINE DIPE VAPOR CONDENSATE.

b 

## MEAN AND STANDARD DEVIATIONS WERE CALCULATED USING THE TOTAL NUMBER OF ABNORMAL SPERM

AS A PERCENTAGE OF THE NUMBER OF SPERM EXAMINED.

* 

## SIGNIFICANTLY DIFFERENT FROM CONTROL GROUP (p<0.05). 

# Page 559

### 11



## HLS STUDY NO.: 00-4205

APPENDIX A

GASOLINE DIPE VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT,

SPERM MOTILITY AND SPERM COUNT DATA

Group I: 0 mg/m 3 

## (GASOLINE DIPE VAPOR CONDENSATE)

S P E R M C O U N T

Animal Caudal Motility Caudal Homogenization Resistant

No. Epididymis Epididymal Testicular

Weight (g) (%) (million sperm/gram)



## 1026 0.21 a 

## 956.0 105.2

1027 0.30 94 794.7 104.6

1028 0.26 97 764.1 109.9

1029 0.27 95 650.6 108.1

1030 0.29 99 973.5 107.1

1031 0.19 87 1012.6 132.1

1032 0.28 91 952.3 96.6

1033 0.25 98 639.9 81.8

1034 0.23 0 b 

## 363.7 62.1

1035 0.27 93 832.6 89.1

1036 0.27 87 677.7 104.0

1037 0.22 96 945.8 123.6

1038 0.26 95 631.4 105.3

1039 0.21 97 1080.5 101.0

1040 0.29 99 894.2 94.4

1041 0.27 96 855.9 92.1

1042 0.22 85 632.1 101.5

1043 0.26 78 876.7 99.8

1044 0.23 94 1113.8 100.2

1045 0.24 99 975.9 105.2

1046 0.28 96 1116.6 71.7

1047 0.24 94 758.1 107.2

1048 0.29 0 c 

## 501.2 131.7

1049 0.32 88 937.8 77.6

1050 0.28 98 952.3 113.8

1051 0.23 98 731.9 104.6

a 

## TECHNICAL ERROR; SPERM MOTILITY IMAGES WERE NOT SAVED.

b 

## FEWER THAN 25 SPERM CELLS COUNTED IN 5 FIELDS ANALYZED; EXCLUDED FROM

STATISTICAL ANALYSIS.

c 

## FEWER THAN 50 SPERM CELLS COUNTED IN 5 FIELDS ANALYZED. 

# Page 560

### 12



## HLS STUDY NO.: 00-4205

APPENDIX A

GASOLINE DIPE VAPOR CONDENSATE:

A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATS

INDIVIDUAL CAUDAL EPIDIDYMIS WEIGHT,

SPERM MOTILITY AND SPERM COUNT DATA

Group IV: 20,000 mg/m 3 

## (GASOLINE DIPE VAPOR CONDENSATE)

S P E R M C O U N T

Animal Caudal Motility Caudal Homogenization Resistant

No. Epididymis Epididymal Testicular

Weight (g) (%) (million sperm/gram)



## 4026 0.26 a 

## 896.8 109.9

4027 0.30 94 798.2 101.7

4028 0.26 89 929.0 102.8

4029 0.30 97 885.3 96.9

4030 0.25 92 807.2 86.1

4031 0.27 79 542.2 113.4

4032 0.26 90 969.2 100.6

4033 0.30 99 864.4 121.4

4034 0.26 94 916.9 125.5

4035 0.30 76 798.2 118.5

4036 0.25 94 924.3 107.1

4037 0.30 88 1017.8 77.8

4038 0.27 98 747.4 89.5

4039 0.30 98 996.8 87.2

4040 0.27 99 844.3 116.4

4041 0.26 96 925.0 93.9

4042 0.25 97 1535.0 109.2

4043 0.29 97 1139.4 100.4

4044 0.25 93 1012.2 99.6

4045 0.34 98 1125.6 94.2

4046 0.25 96 836.5 117.1

4047 0.26 85 1065.7 91.0

4048 0.27 92 848.1 113.5

4049 0.26 98 756.1 106.7

4050 0.25 96 974.5 107.4

4051 0.29 95 969.9 112.4

a 

## TECHNICAL ERROR; SPERM MOTILITY IMAGES WERE NOT SAVED. 

# Page 561HLS STUDY NO.: 00-4205APPENDIX BGASOLINE DIPE VAPOR CONDENSATE:A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL SPERM MORPHOLOGY DATA13Group I: 0 mg/m3(GASOLINE DIPE VAPOR CONDENSATE)Animal ----------------H e a d---------------- --------T a i l--------No. Normal Amorphous Small Enlarged Double Coiled Bent Double Other1026 20000 000 0 0 01027 19910 000 0 0 01028 19730 000 0 0 01029 20000 000 0 0 01030 19901 000 0 0 01031 20000 000 0 0 01032 19721 000 0 0 01033 19820 000 0 0 01034 191810 000 0 0 01035 19910 000 0 0 01036 19730 000 0 0 01037 20000 000 0 0 01038 20000 000 0 0 01039 19622 000 0 0 01040 19910 000 0 0 01041 20000 000 0 0 01042 19730 000 0 0 01043 20000 000 0 0 01044 19640 000 0 0 01045 19721 000 0 0 01046 20000 000 0 0 01047 19910 000 0 0 01048 321680 000 0 0 01049 19460 000 0 0 01050 20000 000 0 0 01051 19901 000 0 0 0Page 562HLS STUDY NO.: 00-4205APPENDIX BGASOLINE DIPE VAPOR CONDENSATE:A ONE-GENERATION WHOLE-BODY INHALATION REPRODUCTION TOXICITY STUDY IN RATSINDIVIDUAL SPERM MORPHOLOGY DATA14Group IV: 20,000 mg/m3(GASOLINE DIPE VAPOR CONDENSATE)Animal ----------------H e a d---------------- --------T a i l--------No. Normal Amorphous Small Enlarged Double Coiled Bent Double Other4026 20000 000 0 0 04027 19910 000 0 0 04028 20000 000 0 0 04029 19820 000 0 0 04030 19901 000 0 0 04031 19910 000 0 0 04032 20000 000 0 0 04033 20000 000 0 0 04034 19910 000 0 0 04035 20000 000 0 0 04036 19820 000 0 0 04037 20000 000 0 0 04038 20000 000 0 0 04039 20000 000 0 0 04040 19910 000 0 0 04041 20000 000 0 0 04042 19910 000 0 0 04043 19811 000 0 0 04044 20000 000 0 0 04045 20000 000 0 0 04046 19910 000 0 0 04047 20000 000 0 0 04048 19640 000 0 0 04049 19820 000 0 0 04050 20000 000 0 0 04051 20000 000 0 0 0Page 563

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Certificates of Analysis Appendix BB

1. Feed Certificates of Anal ysis ....................................................................... 565

2. Water Certificat es of A nalysis ..................................................................... 579

3. Bedding Certificat es of A nalysis .................................................................. 591

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Historical Control Data

Preface Appendix DD

CD Rat - Mating, Fertil ity and Gest ation ........................................................... 631

CD Rat - Summary of M ean Pup Organ We ights ............................................. 632Huntingdon Life Sciences00-4205Page 631211-DIPE-1GFinalReportHistorical Control DataCD Rat - Mating, Fertility and Gestation Appendix DDFemalesStudy Initiation Paired w/ MatedMatingIndex PregnantFertilityIndexNo. withLivebornGestationIndexID Date MalesNo. No. % No. % No. %00-4202 22-Aug-01 26 26 100 24 92.3 24 10000-4203 21-Jan-02 26 26 100 25 96.2 25 10000-4204 19-Mar-02 26 26 100 25 96.2 25 10000-4206 20-Aug-02 26 24 92.3 21 87.5 21 10000-4207 P0 30-Apr-01 262596.224962410000-4207F1 26 25 96.2 25 100 25 10000-4208P0 12-Jun-01 26 24 92.3 23 95.8 23 10000-4208F1 26 23 88.5 21 91.3 21 100MEAN25 95.7 24 94.4 24 100.0SD1 4.3 2 3.9 2 -N8 8 8 8 8 8MIN23 88.5 21 87.5 21 100.0MAX26 100.0 25 100.0 25 100.0Huntingdon Life Sciences00-4205Page 632211-DIPE-1GFinalReportMalesHistorical Control DataCD Rat - Summary of Mean Pup Organ WeightsAppendix DDTBW=Terminal Body Weight; ORG=Organ WeightStudyTBW BrainSpleenThymusID Wt. ORG/TBW WT ORG/TBW ORG/BRAIN WT ORG/TBW ORG/BRAIN(g) (g) (%) (g) (%) (%) (g) (%) (%)00-4202 Mean 82.3 1.5825 1.9312 0.3245 0.3951 20.514 0.3586 0.4392 22.655S.D. 6.4 0.0629 0.1263 0.0500 0.0588 3.142 0.0547 0.0512 3.381N22 22 22 22 2222 22 222200-4203 Mean 81.8 1.6118 1.9785 0.3445 0.4210 21.381 0.3673 0.4499 22.754S.D. 6.8 0.0713 0.1305 0.0477 0.0472 2.845 0.0887 0.1105 5.300N25 25 25 25 2525 25 252500-4204 Mean 77.3 1.5633 2.0568 0.3173 0.4135 20.303 0.3514 0.4542 22.378S.D. 11.8 0.0905 0.2632 0.0517 0.0550 3.196 0.0771 0.0633 4.168N13 13 13 13 1313 13 131300-4206 Mean 80.2 1.5485 1.9522 0.3380 0.4231 21.822 0.3570 0.4443 22.987S.D. 9.8 0.0990 0.2116 0.0515 0.0528 3.068 0.0691 0.0614 3.914N20 20 20 20 2020 20 202000-4207 Mean 72.7 1.5939 2.2127 0.2792 0.3850 17.589 0.3238 0.4471 20.489S.D. 8.1 0.1572 0.2845 0.0491 0.0613 3.031 0.0788 0.1076 5.486N23 23 23 23 2323 23 232300-4208 Mean 75.6 1.5634 2.0757 0.3218 0.4258 20.582 0.3322 0.4389 21.234S.D. 5.6 0.0591 0.1267 0.0484 0.0589 3.031 0.0600 0.0718 3.687N22 22 22 22 2222 22 2222Overall Mean 78.4 1.5797 2.03430.3211 0.4100 20.349 0.3483 0.4451 22.051S.D. 8.6 0.0970 0.2157 0.0533 0.0571 3.293 0.0729 0.0820 4.464N 125 125 125 125 125 125 125 125 125Huntingdon Life Sciences00-4205Page 633211-DIPE-1GFinalReportFemalesHistorical Control DataCD Rat - Summary of Mean Pup Organ WeightsAppendix DDTBW=Terminal Body Weight; ORG=Organ WeightStudyTBW BrainSpleenThymusID Wt. ORG/TBW WT ORG/TBW ORG/BRAIN WT ORG/TBW ORG/BRAIN(g) (g) (%) (g) (%) (%) (g) (%) (%)00-4202 Mean 76.7 1.5323 2.0070 0.2884 0.3741 18.875 0.3836 0.5005 25.068S.D. 5.5 0.0671 0.1472 0.0681 0.0702 4.548 0.0468 0.0486 3.049N22 22 22 22 2222 22 222200-4203 Mean 75.1 1.5506 2.0750 0.3043 0.4041 19.597 0.3459 0.4384 22.301S.D. 6.4 0.0631 0.1576 0.0559 0.0606 3.307 0.0466 0.1045 2.830N25 25 25 25 2525 25 252500-4204 Mean 68.4 1.4423 2.1509 0.2905 0.4296 20.168 0.3468 0.5089 24.529S.D. 11.5 0.1499 0.3410 0.0588 0.0771 3.510 0.0808 0.0917 8.364N13 13 13 13 1313 13 131300-4206 Mean 73.9 1.5185 2.0712 0.3023 0.4107 19.934 0.3484 0.4700 22.918S.D. 7.0 0.0805 0.1894 0.0440 0.0545 2.928 0.0588 0.0550 3.650N20 20 20 20 2020 20 202000-4207 Mean 67.4 1.5015 2.2454 0.2440 0.3613 16.388 0.3253 0.4869 21.854S.D. 6.9 0.1191 0.2698 0.0473 0.0564 3.713 0.0884 0.1423 6.683N23 23 23 23 2323 23 232300-4208 Mean 69.9 1.5284 2.1971 0.2831 0.4058 18.506 0.3420 0.4891 22.349S.D. 5.9 0.0688 0.1724 0.0428 0.0573 2.541 0.0607 0.0805 3.704N22 22 22 22 2222 22 2222Overall Mean 72.2 1.5181 2.12320.2849 0.3950 18.8008 0.3485 0.4796 23.044S.D. 7.7 0.0946 0.2243 0.0563 0.0647 3.6457 0.0655 0.0950 4.858N 125 125 125 125 125 125 125 125 125Huntingdon Life Sciences00-4205Page 634211-DIPE-1GFinalReportCombinedHistorical Control DataCD Rat - Summary of Mean Pup Organ WeightsAppendix DDTBW=Terminal Body Weight; ORG=Organ WeightStudyTBW BrainSpleenThymusID Wt. ORG/TBW WT ORG/TBW ORG/BRAIN WT ORG/TBW ORG/BRAIN(g) (g) (%) (g) (%) (%) (g) (%) (%)00-4202 Mean 79.5 1.5574 1.9691 0.3064 0.3846 19.694 0.3711 0.4698 23.862S.D. 4.9 0.0538 0.1141 0.0498 0.0494 3.371 0.0405 0.0353 2.678N22 22 22 22 2222 22 222200-4203 Mean 78.5 1.5812 2.0267 0.3244 0.4120 20.489 0.3566 0.4442 22.528S.D. 6.0 0.0561 0.1306 0.0467 0.0465 2.769 0.0607 0.0832 3.606N25 25 25 25 2525 25 252500-4204 Mean 72.9 1.5028 2.1039 0.3039 0.4216 20.235 0.3491 0.4816 23.454S.D. 11.2 0.0909 0.2906 0.0526 0.0621 3.167 0.0701 0.0556 5.198N13 13 13 13 1313 13 131300-4206 Mean 77.0 1.5335 2.0117 0.3202 0.4169 20.878 0.3527 0.4572 22.953S.D. 7.5 0.0829 0.1836 0.0374 0.0435 2.398 0.0563 0.0471 3.298N20 20 20 20 2020 20 202000-4207 Mean 70.1 1.5477 2.2290 0.2616 0.3731 16.989 0.3246 0.4670 21.171S.D. 6.9 0.1082 0.2357 0.0423 0.0525 2.746 0.0732 0.1140 4.782N23 23 23 23 2323 23 232300-4208 Mean 72.8 1.5459 2.1364 0.3024 0.4158 19.544 0.3371 0.4640 21.792S.D. 4.8 0.0504 0.1348 0.0391 0.0510 2.453 0.0501 0.0682 3.115N22 22 22 22 2222 22 2222Overall Mean 75.3 1.5489 2.07870.3030 0.4025 19.575 0.3484 0.4623 22.548S.D. 7.5 0.0771 0.2001 0.0486 0.0526 3.057 0.0597 0.0735 3.819N 125 125 125 125 125 125 125 125 125

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Testing Facility Personnel Appendix EE

TITLE/DEPARTMENT NAME/DEGREE

SENIOR VICE PRESIDENT,

SAFETY ASSESSMENT Sylvie J. Gosselin, D.V.M., Ph.D.,

Diplomate A.C.V.P.

SENIOR DIRECTOR, SAFETY

ASSESSMENT Carol S. Auletta, M.B.A., D.A.B.T.,

R.A.C.

DIRECTOR, TOXICOLOGY

OPERATIONS Ian Vanterpool, F.I.A.T.

DIRECTOR, PATHOLOGY SERVICES Barbara A. Litzenberger, B.S.,

M.T. (ASCP)

DIRECTOR, QUALITY ASSURANCE Nicki S. Iacono, B.S.

STUDY DIRECTOR Gary M. Hoffman, B.A., D.A.B.T.

PATHOLOGIST Katharine M. Whitney, D.V.M.,

Ph.D., Diplomate A.C.V.P.

STUDY MONITOR Ronald L. Brzozowski, B.A.,

AALAS LAT

VETERINARIAN Teresa S. Kusznir, V.M.D.

MANAGER/SUPERVISOR

Rodent/Inhalation Toxicology and

Pharmacy

Reproductive Toxicology

Analytical Services

Histology Ellen H. Whiting, AALAS LAT

AALAS LAT

Robert A. Faust, B.S.

Kay Saladdin, B.S.

Janet Kusisto

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Report Amendments Appendix FF

There are no amendments for this report at this time.