PUBLIC COPY p. 1 

Form Approved. O.MB. No. 2.070-0012. Approval Expires 10-31-96. 

U.S. ENVIRONMENTAL PROTECTION AGENCY 

... _ 1111 PREMANUFACTURE 

NOTICE 

NEW CHEMICAL SUBSTANCES 

DOCUMENTCONTROLOFRCER 

OFFICE OF POLLUTION PREVENTION 

AND TOXIC SUBSTANCES, 7407 

U.S. E.P.A. 401 M STREET, SW 

WASHINGTON, D.C. 20460 

Enter the total number of pages 

in the Premanufacture Notice 

COMPANY SANITIZED 

•You must provide all information requested in this form to the exb!llt that it is known to or reuonably ascertainable by you. Make reasonable estimates if you do 

not have actual data. ' 

• Before you complete this form, you should read the "Instructions Manual for Premanufacture Notification" (the Instructions Manual is available from the Toxic 

Substances Control Act (TSCA) Information Service by calling 202~554-1404, or faxing 202-554-5603). 

•If a user fee has been remitted for this notice (40 CFR 'ioo.45), indicate in the boxes above the TS-user fee identification number you have generated. 

Remember, your user fee ID number must also appear on your corresponding fee remittance, which is sent to: EPA, HQ Accounting Operations Branch (PM-266), 

P.O. 360399M, Pittsburgh, PA 15251-6399, Attn. TSCA User Fee. 

Part I- GENERAL INFORMATION 

You must provide the currently correct Chemical Abstracts (CA) Name of the 

new chemical substance, even if you claim the identity as confidentiaL You may 

authorize another person to submit chemical identity information for you, but 

your submission will not be complete and the review will not begin until EPA 

receives this information. A letter in support of your submission should 

reference your TS user fee identification number. You must submit an original 

and two copies of this notice including all test data. If you claimed any 

information as confidential, a single sanitized copy must also be submitted. 

Part II- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

If there are several manufacture, processing, or use operations to be described in 

Part II, sections A and B of this notice, reproduce the sections as needed. 

Part III- LIST OF ATTACHMENTS 

Attach additional sheets if there is not enough space to answer a question fully. 

Label each continuation sheet with the corresponding section heading. In Part 

III, list these attachments, any test data or other data and any optional 

information included in the notice. 

OPTIONAL INFORMATION 

You may include any information that you want EPA to consider in evaluating 

the new substance. On page 11 of this form, space has been provided for you to 

descn"be pollution prevention and recycling information you may have regarding 

the new substance. 

So-called "binding" boxes are included throughout this form for you to indicate 

your willingness to be bound to certain statements you make in this notic:e. such 

as use, production volume, protective equipment ••• This option is intended to 

reduce delays that routinely accompany the development of consent orders or 

Significant New Use Rules. Except in the case of exemption applications ~uch as 

TMEA, LVE, LOREX) where certain information provided in such notification is 

binding on the submitter when the Agency approves the exemption application, 

checking a binding box in this notice~ by itself prohibit the submitter 

from later deviating from the information (except chemical identity) reported in 

the form. 

CONFIDENTIALITY CLAIMS 

You may claim any information in this notice as confidential. To assert a claim 

on the form. mark (X) the confidential box next to the information that you claim 

as confidential. To assert a claim in an attachment, circle or bracket the 

information you claim as confidential. If you dajm information in the police u 

confidential. you must also proyide a unitizes! vemiop of the notic;e. <ipc;ludin& 

attachments!. For additional instructions on claiming information as 

confidential, read the Instructions Manual. 

~ Mark (X) if any information in this notice is claimed as confidential. 

EPA FORM 7710-25 (Rev. 5-95) 

TEST DATA AND OTHER DATA 

You are required to submit all test data in your possession or control 

and to provide a description of all otker data known to or reasonably 

ascertainable by you, if these data are related to the health and 

environmental effects of the manufacture, processing, distribution 

in commerce, use, or disposal of the new chemical substance. Standard 

literature citations may be submitted for data in the open scientific 

literature. Complete test data <written in Eoslishl pot symmaries of dab 

mgst be submitted if they do not awev in the open literature, You should 

clearly identify whether test data is on the substance or on an analog. Also, 

the chemical composition of the tested material should be characterized. 

Following are examples of test data and other data. Data should be 

submitted according to the requirements of §720.50 of the Premanufacture 

Notification Rule (40 CFR Part 720). 

Test Data (Check Below any included in this notice) 

• Environmental fate data Yes • Other data D Yes 

• Health effects data Yes Risk assessments 

• Environmental effects data Yes Structure/activity relationships 

Test data not in the possession 

or control of the submitter 

• Physical/Chemical Properties • ~ Yes 

• A physical and chemical properties worksheet is located on the last page 

of this form. 

TYPE OF NOTICE (Check Only One) 

D 

D 

rn 

D 

D 

D 

D 

PMN (Premanufacture Notice) 

INTERMEDIATE PMN (submitted in sequence with final product 

PMN) 

SNUN (Significant New Use Notice) 

TMEA (Test Marketing Exemption Application) 

.:: ·:::J 

LVE (Low Volume Exemption) @ 40 CFR '723.50 (c)(1) 1 '"U i;2 c.r; -o .. , LOREX (Low Release/Low Exposure Exemption) @ 723.SO(c)(2T-I f;j 

h -...- LVE Modification 0 LOREX Modificatkill 2)"" '-0 _,..,.., • • ("") t::) 

IS THIS A CONSOLIDATED PMN? 0 Yes f\.) 

I of chemicals 

(Prenotice Communication I required, enter I on page 3) 

Replaces previous editions of EPA Form 7710-25. 

' 
p.2 

Public reporting burden for this collection of information is estimated to average 110 hours per response, including time for reviewing instructiom, 

searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send 

comments regarding the burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, 

to Chief, Information Policy Branch, PM-223, U.S. Environmental Protection Agency, 401 M. St, S.W., Washington, D.C. 20460; and to the Office of 

Management and Budget, Paperwork Reduction Act (2070-0012), Washington, D.C. 20503. 

CERTIFICATION 

I certify that to the best of my knowledge and belief: 

1. The company named in Part L section A, subsection 1a of this notice form intends to manufacture or import for a 

commercial purpose, other than in small quantities solely for research and development, the substance identified in 

Part I, Section B. 

2. All information provided in this notice is complete and truthful as of the date of submission. 

3. I am submitting with this notice all test data in my possession or control and a description of all other data known to 

or reasonably ascertainable by me as required by §720.50 of the Premanufacture Notification Rule. 

Additional Certification Statements: 

If you are submitting a PMN, Intermediate PMN, Consolidated PMN, or SNUN, check the following user fee certification 

statement that applies: 

[!] The Company named in Part I, Section A has remitted the fee of $2500 specified in 40 CFR 700.45 {b), or 

D The Company named in Part I, Section A has remitted the fee of $1000 for an Intermediate PMN (defined@ 40 CFR 700.43) in 

accordance with 40 CFR 700.45(b }, or 

D The Company named in Part I, Section A is a small business concern under 40 CFR 700.43 and has remitted 

a fee of $100 in accordance with 40 CFR 700.45 (b). 

If you are submitting a low volume exemption (LVE) application in accordance with 40 CFR 723.50 (c) (1) or a Low 

release and low exposure exemption (LoREX) application in accordance with 40 CFR 723.50 (c) (2}, check the following 

certification statements: 

D 

D 

D 

The manufacturer submitting this notice intends to manufacture or import the new chemical substance for 

commercial purposes, other than in small quantities solely for research and development, under the terms 

of 40 CFR 723.50. 

The manufacturer is familiar with the terms of this section and will comply with those terms; and 

The new chemical substance for which the notice is submitted meets all applicable exemption conditions. 

D If this application is for an L VEin accordance with 40 CFR 723.50 (c)(1}, the manufacturer intends to commence 

manufacture of the exempted substance for commercial purposes within 1 year of the date of the expiration of the 

30 day review period. 

The accuracy of the statements you make in this notice should reflect your best predictioo of the anticipated facts regarding the chemical 

substance described herein. Any knowing and willful misinterpretation is subject to criminal penalty pursuant to 18 USC 1001. Slgna-;r;; A;_~ Slgnatu .. Requhed) Date 

MANAGER, OCCUPATIONAL HEALTH 

Signature of agent - (if applicable) Date 

FORM EPA m0-25 (Rev. 5-95) Page2 

Confidential 
Part I-- GENERAL INFORMATION 

la. Person 

Submitting 

Notice 

Name of authorized official 

F. Mulherin 

Companv 

Position 

Manager, Occupational Health Fluoroproducts 

(in U.S.) E. I. duPont de Nemours & Company, Inc. 

b. Agent(if 

applicable) 

Mailin2 address (number and street) 

1007 Market Street 

City, State, ZIP Code 

Wilmington, DE 19898 

Name of authorized official 

City, State, ZIP Code Telephone 

c. H you are submitting this notice as part of a joint submission. mark (X) this box. ---------------• 

Joint Name of authorized official J'no;iHnn 

Submitter 

(if applicable) 

Mailing address (number and street) 

City, State, ZIP Code 

Telephone 

2. Technical Name Position 

Contact F. Mulherin 

(in U.S.) 

Manager, Occupational Health Fluoroproducts 

Company 

E. I. du Pont de Nemours & Company, Inc. 

Mallin~~; address (number and street) 

1 007 Market Street 

City, State, ZIP Code 

Wilmington, DE 19898 

3. H you have had a prenotice communication (PC) concerning this notice 

and EPA assigned a PC Number to the notice, enter the number. 

4. H you previously submitted an exemption application for the chemical 

substance covered by this notice, enter the exemption number assigned 

by EPA. H you previously submitted a PMN for this substance enter the 

PMN number assigned by EPA (i.e. withdrawn or incomplete). 

H you have submitted a notice of Bona fide intent to manufacture --·~ or 

import for the chemical substance covered by this notice, enter the 

notice number assigned by EPA :. 

Type of Notice- Mark (X) 

FORM EPA 7710-25 lRev. 5-95) 

t.0 

D 

Manufacture 

Only 

Option 

Page3 

Telephone 1 

I 302 I 

1 774-2193 

PC-1574 

2. D Import 

Only 

Mark (X) 

if none 

Mark (X) 

if none 

Mark (X) 

if none 

D BindingOption 

Mark(x) 

_ ___,.,..~ D 

3. 0 Both 
Part I -- GENERAL INFORMATION - Continued 

Section B- CHEMICAL IDENTI'IY INFORMATION: Youmutprovideacurre~tly blued on the ninth Collective 

Mark (X} the "Confidential" box next to any item you claim as confidential. 

.4 

Abstracts ICA \~·-'~of the substance 

nomenclature rules and conventions. 

Complete either item 1 (Clasa1 or 2 subsbnces) or 2 (Polymers) as appropriate. Complete all other items. 

If another person will submit chemical identity information for you (for either item 1 or 2), mark (X) the box at the ri~t. D 

the and address of that in a continuation sheet. >" 

1. Class 1 or 2 chemical substances (for definitions of class 1 and class 2 substances, see the Instructions ~nual) 

a. Class of substance- Mark (X) 1 [!] Oass 1 or 2 0 Class 2 

b. Chemical name (Currently correct Chemical Abstracts (CA) Name that is consistent with TSCA Inventory listings for similar substances. 

For Class 1 substances a CA Index Name must be provided. For Class 2 substances either a CA Index Name orCA Preferred Name 

be provided, whichever is appropriate based on CA 9CI nomenclature rules and conventions.). 

Pentane, 1, 1, 1,2,3,4,4,5,5,5-

Decafluro 

c. Please identify which method you used to develop or obtain the specified chemical indentity information reported in this notice: 

(check one). 

DMethod 1 (CAS Inventory Expert Service- a copy of the Identification ~ethod 2 (Other Source) 

report obtained from the CAS Inventory Expert Service must be 

submitted as an attachment to this 

d. Molecular formula and CAS Registry Number (if a number already exists for the substance) 

CF3

CFHCFHCF2

CF3 CAS I 

138495-42-8 

e. For a class 1 substance, provide a complete and correct chemical structure diagram. For a class 2 substance- (1) List the immediate 

precursor substances with their respective CAS Registry Numbers. (2) Describe the nature of the reaction or process. (3) Indicate the 

range of composition and the typical composition (where appropriate). (4) Provide a correct representative or partial chemical 

structure diagram, as complete as can be known, if one can be reasonably ascertained. 

F 

F- C 

F 

F 

c 

H 

F 

c 

H 

Mark (X) this box if you attach a continuation sheet. 

FORM EPA m0-25 (Rev. ~95) 

F 

c 

F 

F 

C- F 

F 

Page4 

HFC-43-lOm.ee 
p.5 

Part I --GENERAL INFORMATION -- Continued 

~ Section B - CHEMICAL IDENTITY INFORMATION - Continued 

2. Polymers (For a definition of polymer, see the Instructions Manual.) Confidential 

a. Indicate the number-average weight of the lowest molecular weight composition of the polymer you intend to manufacture. 

Indicate maximum weight percent of low molecular weight species (not including residual monomers, reactants, or solvents) below 

500 and below 1,000 absolute molecular weight of that composition. 

Describe the methods of measurement or the basis for vour estimates: GPC 0 Other0 : (Specify) 

i) lowest number average molecular weight 

ii) maximum weight% below 500 molecular weight NOT APPLICABLE 

iii) maximum weight% below 1000 molecular weight 

0 Mark (X) this box if y<>_u attach a continuation sheel 

b. You must make separate confidentiality claims for monomer or other reactant identity, composition information, and residual information. 

Mark (X) the "Confidential" box next to any item you claim as confidential. 

(1) - Provide the specific chemical name and CAS Registry Number (if a number exists) of each monomer or other reactant used in the 

manufacture of the polymer. 

(2) - Mark (X) this column if entry in column (1) is confidential 

(3) - Indicate the typical weight percent of each monomer or other reactant in the polymer. 

(4) - Mark (X) the identity column if you want a monomer or other reactant used at two weight percent or le• to be listed as part of the 

polymer description on the TSCA Chemical Substance Inventory. 

(5) - Mark (X) this column if entries in columns (3) and (4) are confidential. 

(6) - Indicate the maximum weight percent of each monomer or other reactant that may be present as a residual in the polymer as 

manufactured for commercial purposes. 

(7) - Mark (X) this column if entry in column (6) is confidential. 

Monomer or other reactant and CAS Registry Number Confi- Typical Identity Confi- Maximum Confidential r~;..ootinn Mark (X) dential residual dential 

(1) (2) .(3) (4) (5) (6) (7) 

% % 

% % 

% % 

% % 

% % 

% Of_ 

% % 

0 Mark (X) this box if you attach a continuation sheet. 

c. Please identify which method you used to develop or obtain the specified chemical identity information reported in this notice. (check one). 

0 Method 1 (CAS Inventory Expert Service- a copy of the identification report 0 Method 2 (other source) 

obtained from CAS Inventory Expert Service must be submitted as 

an attachment to this notice)· 

d The currently correct Chemical Abstracts (CA) name for the polymer that is consistent with TSCA Inventory listings for similar polymers. 

e. Provide a correct representative or partial chemical structure diaJUam, as complete as can be known, if one can be reasonably ascertained. 

0 Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (R ev. 5-95 PageS 
,. 

• 

p.G 

Part I -GENERAL INFORMATION --Continued 

~ Section B- CHEMICAL IDENTITY INFORMATION - Continued 

3. Impurities 

(a) - Identify each impurity that may be reasonably anticipated to be present in the ch-icalsubstance • manufactured for commercial purposes. 

Provide the CAS Registry Number if available. If there are unidentified impurities, enter "unidentified." 

(b) -Estimate the muimum weight •;. of each impurity. If there are unidentified impurities, estimate their total weight •;.. 

Impurity and CAS Registry Number Maximum Confi- percent 

(a) (b) dential 

1,1,1,2,4,4,5,5,5 Nonafluoropentane 141993-32-Q <1 % 

1,1,1,3,4,4,5,5,5 Nonafluoropentane 141993-31-9 <1 % 

·- f-pentene-2 (CF3 CF=CFCF2CF j 72804-49-0 .05 % . 

% 

% 

% 

% 

0 Mark_ (X) this box if you attach a continuation sheet. 

4. Synonyms-- Enter any chemical synonyms for the new chemical substance identified in subsection 1 or 2. 

~:=~~ HFC-43-10mee 

0 Mark (X) this box if you attach a continuation sheet. 

5. Trade identification- List trade names for the new chemical substance identified in subsection 1 or 2. 

Hydrofluorocarbon 

0 Mark (X) this box if you attach a continuation sheet. 

6. Generic chemical name- If you claim chemical identity as confidential. you must provide a generic chemical name for your substance 

that reveals the specific chemical identity of the new chemical substance to the maximum extent possible. 

Refer to the TSCA Chemical Substance Inventory, 1985 Edition, Appendix B for guidance on developing 

generic names. 

0 Mark (X) this box if you attach a continuation sheet. 

7. Byproducts '"' any byproducts resulting from the manufacture, processing, use, or disposal of the new chemical substance. Provide the CAS 

Regu;try 1··;uu.....a if available. 

Byproduct CAS Registry Number Confi-

(1) (2) dential 

Hydrogen Fluoride 7664-39-3 

Sodium Fluoride 7681-49-4 

Cf14 

- C7

F

13

CC 

- D Mark (X) this box if you attach a continuation sheel 

FORM EPA 7710-25 (Rev. 5-95) Page6 
p. 7 

Part I --GENERAL INFORMATION-- Continued 

~ Section C- PRODUCTION, IMPORT, AND USE INFORMATION: 

Mark (X) the "Confidential" box next to any item you claim as confidential 

1. Production volume -Estimate the maximum production volume during the first 12 months of production. Also estimate the 

maximum production volume for any consecutive 12-month period during the first three years of production. Estimates should be on 

100% new chemical substance basis. For a Low Volume Exemption application, if you choose to have your notice reviewed at a lower 

production volume t1um 10,000 kg!yr, specify the volume and 11Ulrk (x) in the binding box. If granted, you are bound to this volume .. 

Maximum first 12-month ~roduction {kg/yr} Maximum 12-month production (kg/yr} Confi- Bindittg 

dential Optioll (100% new chemical su stance basis) (100% new chemical substance basis) Muk(x) 

x.· 

2. Use Information - You must make separate confidentiality claims for the description of the category of use, the percent of production volume 

devoted to each category, the formulation of the new substance, and other use information. Mark (X) the "Confidential" Box next to any item you 

claim as confidential. 

a. (1) -Describe each intended category of use of the new chemical substance by function and application. 

(2) - Mark (X) this column if entry in column (1) is confidential business information (CBI). 

(3) - Indicate your willingness to have the information provided in column (1) binding. 

(4) -Estimate the percent of total production for the first three years devoted to each category of use. 

(5) -Mark (X) this column if entry in column (4) is confidential business information (CBI). 

(6) -Estimate the percent of the new substance as formulated in mixtures, suspensions, emulsions, solutions, or gels 

as manufactured for commercial purposes at sites under your control associated with each category of use. 

(7) -Mark (X) this column if entry in column (6) is confidential business information (CBI). 

(8)- Indicate %of product volume expected for the listed "use" sectors. Mark more than one box if appropriate. Mark 

(X) to indicate your willingness to have the use type provided in (8) binding. 

(9)- Mark (X) this column if entry(ies) in column (8) is (are) confidential business information (CBI). 

Category of use (1) CBI Binding Produc- CBI %in CBI %of substance expected per use CBI 

(by function and application i.e. a dispersive dye for Optioll tion% Form- (8) Mark(x) ulation finishing polyester fibers) :site- {;on-.. Indus- Com- ~Jindins (2) (3) (4) (5) (6) (7) limited sumer trial mercial Option (9) 

% % X 

% % X 

% % l>( 

% % 

% % 

% % 

% % 

•If you have identified a "consumer" use, please provide on a continuation sheet a detailed description of the use(s) of this chemical 

substance in consumerlroducts. In addition include estimates of the concentration of the new chemical substance as expected in 

consumer products an describe the chemical reactions by which this substance loses its identity in the consumer product. 

0 Mark (X) this box if you attach a continuation sheet. 

b. Generic If you claim any category of use description in subsection 2a as confidential, enter a generic description of that 

use category. Read the Instructions Manual for examples of generic use descriptions. 

description 

Contained process, heat transfer, and test media. 

D Mark (X) this box if you attach a continuation sheet. 

3. Hazard Information - Include in the notice a copy of reasonable facsimile of any hazard warning statement, label, material safety data sheet, Binding 

or other information which will be provided to any person who is reasonably likely to be exposed to this substance regarding protective Option 00 (uipment or practices for the safe handling, transport, use, or disposal of the new substance. List in part m hazard information you include. Mark(x) 

Mark ()() this box if_you attach hazard information. 

FORM EPA 7710-25 (Rev. 5-95) Page7 
p.8 

Part II --HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

Complete section A for each type of manufacture, proce~&ing, or use operation involving the new chemical substance at industrial sites you control. 

Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are 

further industrial processing or use operations after import. You must dei!ICribe these operations. See instructions manual. 

of the site at which the willocrur. 

Name 

City, County, State, ZIP Code 

If the same operation will ocrur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different ... 

production rates or operations, include all the information requested in this section for those 

b. TypeMark (X) 

attach a continuation sheet 

0 Manufacturing 

1. Batch 

2. Continuous 

D Processing 

Hours/batch 

Hours/dav 

0 Use 

Batches/vear 

Days/year 

d. Process description Mark (X} to indicate your willingness to have your process deKription binding. 

(1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify-e.g. 5 gallon pails, 55 

gallon drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/ day or kg/batch on an 100% new chemical substance basis), and entry point of all starting 

materials and feedstocks (including reactants, solvents, and catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning 

chemicals (note frequency if not used daily or per batch.). 

(3) Identify by number the points of release, including small or intermittant releases, to the environment of the new chemical substance. 

W Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (Rev. 5-95) Page SA 
p.9 

Part II --HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

Section A- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER 

Complete section A for each type of manufacture, proce11ing, or use operation involving the new chemical substance at industrial sites you control. 

Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are 

further industrial processing or use operations after import. You must describe these operations. See instructions manual. 

of the site at which the will occur. 

Name 

Site address (number and street) 

City, County, State, ZIP Code 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different > 

production rates or operations, include all the information requested in this section for those 

b. TypeMark (X) 

attach a continuation sheet 

0 Manufacturing 

1. Batch 

2. Continuous 

D Processing 

Hours/batch 

Hours/dav 

0 Use 

Batches/ vear 

Days/year 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

X 

(1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify-e.g. 5 gallon pails, 55 

gallon drum. rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/ day or kg/batch on an 100% new chemical substance basis), and entry point of all starting 

materials and feedstocks (including reactants, solvents, and catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning 

chemicals (note frequency if not used daily or per batch.). 

(3) Identify by number the points of release, including small or intermittant releases, to the environment of the new chemical substance. 

D Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (Rev. 5-95) PageS B 
\.iOntatns Confidential 

Business Information 

p.10 
\Jontatns Confidential 

Business Information 

If 

p. 11 
· 0 c t. 2 5. 19 9 6 1 0 : 3 4 AM DUPONT No. 4342 

Part II- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE- Continued 

~ Section A - INDUSTRIAL SITES CONTROLLED BY THE SUBMITrER- Continlled 

1. Occupational Exposure- You m~t make separate con.lid~ty claims for~ de:scripli_~,ofworker activity, physical ~rm of the new .chemical . substance, number of workers exposed. and duration of actiVlty. Mark (X) the Confidential box I\I!Xt to any 1tem. you claim as confidential. 

(1)- Describe the activities (e.g. bag dl.lDlping, tote .lilliiig, unloading drums, sampling, clearUI\g. etc.) in which workers may be expasecl. to the 

substance. 

(2) -Mark (X) this column if entry in colWNI (1) is confidential busiJI.ess infonnation (CBI). 

(3) - Describe any protective equipment a.nd engiMering controls used to protect workets. 

(4) and (6) - Indicate you williJ1glles& to have the infomla.tion provided in colwm (3) or (5) binding. 

(5) -Indicate the physical farm(s) of the rtew chemical substance (e.g. solid: crystal granule, powder, or dust) and% new chemical substance (if part of 

a mixture) at the time of exposUJ"e. 

~7) - Mark (X) this colwnn il entry in column (5) is confidential business intonnation (CBI). 

8) - Estimate the maximW"n number of workers involved in each activity ford sites combined. 

(9) -Mark (X) thiscolwnnilenltyin col\ID\I\ (8) is conlidential buslnessi.nlonnati.on (CBI). 

(10) and (11) - Estimate the maximum dUJ"ation of the activity for any worker in hoiUS per day and days per year. 

(12) - Mark (X) this column if entries in colUDIJIS (10) and (11} are con5dential business infonnation (CBl). 

Worker activity CBI ProtectiYe E<t alpaeatf. llidins: l'ftysJc.al tonnt~J 

~· CBI tof CBI Mmmum duration CBI 

(e.g. bag dumping. fillitlg drums) bgiaccriaiJ Coatrols Optloa (4!.g.solid:powdar) 

~be) Worken ~(x) m4%new Exposed Hr.;/ day Day-5/yr 

m 12) (3) (4) 11Ub5~CI! (..;t {7\ "(6} {9) 110> n1) lnl) 

X Impervious Gloves. X X 1 70 

Respiratory Protection - X· 1 1mpotv1ous ~lOVes, 

Respiratory Protection X X 8 10 

X Impervious Gloves X X 4 10 . 

0 Mark (X) this box if you attach a continuation sheet. 

3. En•ironmental Releaee a11d Dilpotal - You U\USt make separate cOIIfidentiality cWm5 for the release nUD\be:r aad the amount of the n.ew chemical 

su.bsbu\ce released.and·other release and disposal infonnation. Mar.k (X) the "Conficlential" box next to each item you claim as confidential. 

(1) - Enter the number of each release point idenli.6ed in the proce55 descriptio:n,. part II,. section A. subsection ld(3). · . 

~2) - Estimate the amounr of the new substance released (a) directly to the enviroNIW\t or (b) into contr:ol technology (in kg/ day or kg/batch). . 

3) - Mark (X) this c:olUDIX\ if entries in colWMS (1) and (2) are confidential business infonnation (CBI). 

(4) - Identify the media of release i.e. stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or itldnerati.o.a, 

PO'lW, or othe:f (please specify) to which the new substance will. be released lrom that release point · 

(5) - a. Desaibe control technology, if any, ,and contrDl efficiency that will be useclto limit the release of the new subsllmce to the en..;rpnment. 

For releases disposed of on land, characterize the disposal method ancl state whether it is approved for disposal of RCRA hazwdous waste. 

· On a continuation sheet , for each site de~>cribe any additional cl.i5poAl methods that will ~ usecl anci whether the waste is subject to 

secon<Wy or tertiary on-si.te treatment. b. Estimate ~e amount released to the enviroraneat after control techrlology (m kg/ day). 

~6) - Mark (X) this col\lJM if entries in colwn.ns (4) mel (5) are conficlenMJ business i.nformation (CBl}. . . · . . 7) - Identily the destixlation(s) of. releases to water.· Please ~pply NPDES (National Poll~tant Discharge ElimiNtion system) numbers for direct 

di.schargeJS or NPDES numbers of the PO'IW (Publicly Owned Txeatment Works). Mark (X) if the PO'TW name or NPDES t# i:> confidential 

business information (CBI). . 

Release Amount of-new subsW~Ce CBI 1Medl.aof Control technology and efficiency (you may wi&h to optioDally attach effic:iii\Cy data) CBl 

Number I'Q)~wl releilse I~ (1) (2a) (2b) e.JA>Ckair 

(3) (4) .:. (SOl) : M.uk l'<l . (Sb) (6) 

X Water Neutralization System I <.001 k!Jiday ·- 1 I 

I 

2 X Air Incineration I <0.0001 kgfday - .. I 

I 

I 

I 

I 

I ' 

I 

I 

I 

(7) Mark (X) the D P01W pr:ovide l.'lall\e(s) below: CBI C Navigable 0 OOIIr. Specify provi.de NPDES it CBI 

destiauliOn(s) of f-- -~y 

X X releases J water. 

'fl} Mark (X) this box if you attach a continuation sheet 

FORM EPA 7710-25 (Rev. 5-95) Page9 A 

OCT-25-1996 10:36 3027748416 J...7 96% P.02 
l -0 c t. 2 5. 19 9 6 I 0 : 3 4 AM DUPONT No. 4342 .. . Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE- Continued 

~ Section A- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTF.R ··Continued 

2.. Occupational Exp05un -You must INIIte separate con.fidentialiay claims for ahe description of worker .l(tiv\ty, physical form Clf the new chetnical ~~bs~nce. ni.UI"'b~r of worker; ~'<p<'Sed. and dunlion of activity. Mark (X) thl! 'Cilniidtmaal" bo~t nt:~<t w any item y011 c1ailn as conJiclential. 

(1) - De,;~nbo! rho! .l(tivities (t.g. b:~g dumping. late filling. unloacling drums. sampling, cleaning. etc.) in which workers may be exposed to the 

substance. 

(2) - Mark (X) this coli.U\"'n 1f enuy in col \UN\ (1) is confidmti.3.1 business information (CBl). 

(3)- Describe any protective equipmetlt and~ controls used co protect workers. 

(-l) and (6)- (ndieate you willitlgnts.s to 1\:lve the mformation provided in column {3) or (S) binditls-

(S)- Indicate the physical form(s) of lhe new chemical substance (e.g. solid: crystal, grarwle. powder, or dust) and 0

k new chemical substance {if pa-t of 

a mixture) at the time of exposure. · 

(7) - Mark (X) this column ii entry in colUINI (5) is confidential business information (CBI). 

(8) - Esamate the maxi.lnwn number of worlc.ers involved. irl each activity for all sites combined. 

(9) -Mark (X) this colWN\ if entry ir1 colwnn (8) is confidential business information (CBI). 

(10) and (11) - Estunate the maxunum duration of the activity for any worker in hours per day and days per yev. 

02) -Mark (X) this column ii entries in col\liX\IIS (10) and (11) are confidl!l\tial businesS infonnation (CBI). 

Worker activity CBI l'lotcdivc £q11ip111oellll !Jiactiq 1'1\ys,caa torm~J ~illclias CBI I of CBl MalliDJ~ duration CBI 

pptloa (e.g.solid:powder) 

~:c, Worken (e.g. bag dumping. fill.lllg drwns) Eapnec1iR3 Coatrols ~(ll) ancl %new Hrs/lky Days/yr Exposed 

m (2) J3l (4\ subs~e ({,\ 17\ "<8) (9\ (l()) n1\ il12) 

. 'oj X Impervious Glo11es, X X 1 230 

Respiratory Protection 

X ~mpeiVIOUS '!JOV8S, 

RespiraiOiy Proteetion X X 8 40 

X Impervious Gloves X X 4 50 

~ 

0 Mark (X) this bo:t if you attach a continuationsheel. 

3. Environmental Rei ease a.n d D iJpoAI- You must make separate coa&denl:iality daims for the release nwnber mel the amount of lhe new chemica! 

substance released and ocher relea5e and disposal infonMiion. Mark (X) the "Confidential" box next to eachitem~tl claim ascon&lentW. 

(1) - Enter the ni.QI\ber of each release point idez~tified il\ the process desaiptiort. pvt 11.. section A. subseclion 1 (3). 

(2) - E.5timate the amow:tt of the new substaJu:e released (a) directly to lhe enviroxunent or (b) into control tKhrlology ("m kg/ day or kg/batc:h). 

(3) - Mule: (X) this colUDlll if entries in col\UNIS (1) and (2) are confidential busine5s inforxnation (CBI). 

(4) - lclent# the media of release i.e. stack air, fugitive air (optional-see Instruction Manual), SUJface water, on-«1e or off-site larld or i..nanent3on. 

POTW, or other (please specily) to which the new S\ll7stance will be released &om that release point. 

(5) - a. Describe coatrol technology. il any, ,arid control elficiency that will be used to lilllit IN release of th@ new substarv:e to th@ environment. 

For releases dispo.5ed of on land. characterize the disposal method and stale whether it is approved for disposal of RCR.A hazardous waste. 

On a contint&ation sheet , for each site describ@ any additional disposal. methods that will be used and wtll!ther IN waste is subject 10 

seconclary or tertiary o~site treatment. b. Eslimate the amount released to the environment after CCMtrOltedulology (in kg! day). 

(6) - Mark (X) this colwnn i.l en~5 in columns (4) and (5) are conlidez~tial business informalion (CBI). . . 

(7) - Identity the destination(s) of releases to watet. Please Sl.lpply NPOES (National Pollutant Discharge EWNnation System) nuJDbets for clirect 

dischargers or NPDES munbers of the POTW (Publidy Owned Ttealma\t Worlcs). Mark (X) il the POIW t'alllt or NPDES tis con6denlial 

'bus.iness i.n.fonnation (CBI). 

Release Amount ofMW sW5tal\ce CBI Medilof Control technology and elficiency (you may wish to optionaD.y attach effidmcy data) CBI 

Number releued release I llir.dq 

(1) (la) (2b) ... libel< ~lr 

(3) (4) (Sa) : Muk~ (Sb) (6) - - 1 X Water Nat~tralizalion System . I <.001 kgfday (:>99% Efficiency) I 

- I 

2 X Air JncineraliOf\ I <0.0001 kgday 

(:>99% Elficianc:y) I 

L 

I 

I 

1 

I 

I 

I 

I 

I 

(7) Mark (X) the 0 l'OTW provid.e I\IUU!(s) below: CBI []Navigable 0 Od\er - Specify provide NPDES It CBl 

ciestina!ion(s) of 1-- waterway 

releases to water. X X 

0 Mark (X) this box iJ yo1.1 .lttach a COC\tinW~tion sheet. 

- - Page9 B 

OCT-25-1996 10:37 3027748416 /~ 96% P.03 
p.14 

Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

~ Section B - INDUSTRIAL SITES CONTROLLED BY OTHERS 

Complete section B for typical processing or use operations involving the new chemical substance at sites you do not control.Importers do not have to 

complete this section for operations outside the U.S.; however, you must report any processing or use activities after import. See the Instructions 

Manual. Complete a separate section B fur each type of processing, or use oper11tion involving the new chemiall substance. H the same operation is performed 

at more than one site describe the typical operation common to these sites. Identify additional sites on a continuation sheet. 

1. Operation Description - To claim information in this section as confidential, circle or bracket the specific information that you claim as confidential. 

(1) - Diagram the major unit operation steps and chemical conversions, including interim storage and transport containers (specify- e.g. 5 gallon pails, 

55gallon drums, rail cars, tank trucks, etc). On the diagram, identify by letter and briefly describe each worker activity. (2)- Provide the identity, 

the approximate weight (by kg/ day or kg/batch, on an 100% new chemical substance basis), and entry point of all feedstocks (including reactants, 

solvents and catalysts, etc) and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per 

batch). (3) -- Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. 

(4) PI-"""' the• of""" ( -~ ro Hiontify the l=tiDn> of"""' •itos ~ • <~tinua•~"""'~ .,.,.--..., -- #ofSltes 

L--- - 0 Mark (X) this box if you attach a continuation sheet. 

2. Worker Exposure/Environmental Release 

(1)- From the diagram above, provide the letter for each worker activity. Complete 2-8 for each worker activity described. 

(2)- Estimate the number of workers exposed for all sites combined. 

(4)- Estimate the typical duration of exposure per worker in (a) hours per day and (b) days per year. 

(6)- Describe physical form of exposure and% new chemical substance (if in mixture), and any protective equipment and engineering controls used 

to protect workers. 

(7) - Estimate the percent of the new substance as formulated when packaged or used as a final product. 

(9) -- From the process diagram above, enter the number of each release point. Complete 9-13 for each release point identified. 

(10)- Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology to the environment 

(in kg/ day or kg/batch). 

(12)- Describe media of release i.e. stack air, fugitive air (optional-see Instructions Manual), surface water, on-site or off-site land or incineration, 

POTW, or other (specify) and control technology, if any, that will be used to limit the release of the new substance to the environment. 

(14)- Identify byproducts which may result from the operation. 

(3), (5), (8), (11), (13) and (15)- Mark (X) this column if any of the proceeding entries are confidential business information (CBI). 

Letter #of CBI Duration CBI Protective Equip. I '7oin CBI Release Amount of CBI Control CBI 

of Workers of Engineering Controls/ Form- Number New Technology 

Act- Exposed Exposure Physical Form and % ulation Substance 

ivity new substance Released 

I 

(lOa) : (lOb) (1) (2) (3) (4a) I (4b) (5) (6) (7) (8) (9) (11 (12) (13) 

I I 

'I I K X I 

I I y f<" I 

y· I 'X I 

I [>< I IX \ v 

I I / I' --' 

I I 

I I 

I I 

I I 

I I 

I I 

(14)- Byproducts: (15) 

~Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (Rev. 5-95) Page10 A 
p.15 

Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

~ Section B - INDUSTRIAL SITES CONTROLLED BY OTHERS 

Complete section B for typical processing or use operations involving the new chemical substance at sites you do not control.Importers do not have to 

complete this section for operations outside the U.S.; however, you must report any processing or use activities after import. See the Instructions 

Manual. Camplete a separate section B for each type of processing, or use operation involving the new chemical substance. lf the same operation is performed 

at more than one site describe the typical operation common to these sites. Identify additional sites on a continuation sheet. 

1. Operation Description - To claim information in this section as confidential, circle or bracket the specific information that you claim as confidential. 

(1) -Diagram the major unit operation steps and chemical conversions, including interim storage and transport containers (specify- e.g. 5 gallon pails, 

55 gallon drums, rail cars, tank trucks, etc). On the diagram, identify by letter and briefly describe each worker activity. (2)- Provide the identity, 

the approximate weight (by kg/ day or kg/batch, on an 100% new chemical substance basis), and entry point of all feedstocks (including reactants, 

solvents and catalysts, etc) and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per 

OOI<h). (3) -ldontifyby mnnbo< tho,-., of,..,.,., lncludmg ....U ~ mtenn!ttent.-el~~. to lho ~~ oh..U<ol '"""""' 

( 4) Please enter the # of sites ( remember to identify the locations of these sites on a continuation sheet) : ~ 

r- #otstt 

0onta1ns Confidential 

Business Information 

l..---' "'-... 

0 Mark (X) this box if you attach a continuation sheet. 

2. Worker Exposure/Environmental Release 

(1)- From the diagram above, provide the letter for each worker activity. Complete 2-8 for each worker activity described. 

(2) - Estimate the number of workers exposed for all sites combined. 

(4)- Estimate the typical duration of exposure per worker in (a) hours per day and (b) days per year. 

(6) -- Describe physical form of exposure and% new chemical substance (if in mixture), and any protective equipment and engineering controls used 

to protect workers. 

(7) - Estimate the percent of the new substance as formulated when packaged or used as a final product. 

(9) - From the process diagram above, enter the number of each release point. Complete 9-13 for each release point identified. 

(10)- Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology to the environment 

(in kg/ day or kg/batch). 

(12)- Describe media of release i.e. stack air, fugitive air (optional-see Instructions Manual), surface water, on-site or off-site land or incineration, 

POTW, or other (specify) and control technology, if any, that will be used to limit the release of the new substance to the environment. 

(14)- Identify byproducts which may result from the operation 

(3), (5), (8), (11), (13) and (15)- Mark (X) this column if any of the proceeding entries are confidential business information (CBI). 

Letter #of CBI Duration CBI Protective Equip. I !%in CBI Release Amount of CBI Control CBI 

of Workers of Engineering Controls/ Form- Number New Technology 

Act- Exposed Exposure Physical Form and % ulation Substance 

ivity new substance Released 

I 

(1) (lOa) : (lOb) (2) (3) (4a) I (4b) (5) (6) (7) (8) (9) (11 (12) (13) 

I I 

y I 

~ x I x k I I I 

I I 

I I 

I I 

I I 

I I 

I I 

I I 

I I 

I I 

(14) - Byproducts: (15) 

~Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (Rev. 5-95) Page 10 B 

/~ 
p.16 

Part II -- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE --Continued 

~ Section B - INDUSTRIAL SITES CONTROLLED BY OTHERS 

Complete section B for typical processing or use operations involving the new chemical substance at sites you do not control Importers do not have to 

complete this section for operations outside the U.S.; however, you must report any processing or use activities after import. See the Instructions 

Manual. Ccnnplete a separate section B for each type of processing, or use operation involving the new chemical substance. H the same operation is performed 

at more than one site describe the typical operation common to these sites. Identify additional sites on a continuation sheet. 

1. Operation Description - To claim information in this section as confidential, circle or bracket the specific information that you claim as confidential. 

(1) - Diagram the major unit operation steps and chemical conversions, including interim storage and transport containers (specify- e.g. 5 gallon pails, 

55gallon drums, rail cars, tank trucks, etc). On the diagram, identify by letter and briefly describe each worker activity. (2)- Provide the identity, 

the approximate weight (by kg/ day or kg/batch, on an 100% new chemical substance basis), and entry point of all feedstocks (including reactants, 

solvents and catalysts, etc) and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per 

batch). (3) -- Identify by number the points of release, including small or intermittent releases, to the environmen~ the new chemical substance. 

(4) Please enter the# of sites (remember to identify the locations of these sites on a continuation sheet) : 

71 ol sttes -~ 

vonta1ns Confidential 

L---

Business Information "--

0 Mark (X) this box if you attach a continuation sheet. 

2. Worker Exposure/Environmental Release 

(1)- From the diagram above, provide the letter for each worker activity. Complete 2-8 for each worker activity described. 

(2)- Estimate the number of workers exposed for all sites combined. 

(4)- Estimate the typical duration of exposure per worker in (a) hours per day and (b) days per year. 

(6)- Describe physical form of exposure and% new chemical substance (if in mixture), and any protective equipment and engineering controls used 

to protect workers. 

(7) -- Estimate the percent of the new substance as formulated when packaged or used as a final product. 

(9) - From the process diagram above, enter the number of each release point. Complete 9-13 for each release point identified 

(10)- Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology to the environment 

(in kg/ day or kg/batch). 

(12)- Describe media of release i.e. stack air, fugitive air (optional-see Instructions Manual), surface water, on-site or off-site land or incineration, 

POTW, or other (specify) and control technology, if any, that will be used to limit the release of the new substance to the environment. 

(14)- Identify byproducts which may result from the operation 

(3), (5), (8), (11), (13) and (15)- Mark (X) this column if any of the proceeding entries are confidential business information (CBI). 

Letter #of CBI Duration CBI Protective Equip. I %in CBI Release Amount of CBI Control CBI 

of Workers of Engineering Controls/ Form- Number New Technology 

Act- Exposed Exposure Physical Form and % ulation Substance 

ivity new substance Released 

I 

(1) (2) (3) (4a) (lOa) : (lOb) I (4b) (5) (6} (7) (8) (9) (11 (12) (13) 

I I 

v I IX' I 

I [><. I )< >< I 

I I 

I I 

I I 

I I 

I I 

I I 

I I 

I I 

I I 

(14) - Byproducts: (15) 

0 Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (Rev. 5-95) Page 10 C 

16 
p.17 

OPTIONAL POLLUTION PREVENTION INFORMATION 

To claim information in this section as confidential circle or bracket the specific information that you claim as confidential. 

In this section you may provide information not reported elsewhere in this form regarding your 

efforts to reduce or minimize potential risks associated with activities surrounding 

manufacturing, processing, use and disposal of the PMN substance. Please include new 

information pertinent to pollution prevention, including source reduction, recycling activities 

and safer processes or products available due to the new chemical substance. Source reduction 

includes the reduction in the amount or toxicity of chemical wastes by technological 

modification, process and procedure modification, product reformulation, raw materials 

substitution, and/ or inventory control. Recycling refers to the reclamation of useful chemical 

components from wastes that would otherwise be treated or released as air emissions or water 

discharges, or land disposal. Descriptions of pollution prevention, source reduction and 

recycling should emphasize potential risk reduction subsequent to compliance with existing 

regulatory requirements and can be either quantitative or qualitative. The EPA is interested in 

this information to assess overall net reductions in toxicity or environmental releases and 

exposures, not the shifting of risks to other environmental media or non-environmental areas 

(e.g., occupational or consumer exposure). In addition, information on the relative cost or 

performance characteristics of the PMN substance to potential alternatives may be provided. 

All information provided in this section will be taken into consideration during the review 

of this substance. See the revised Instructions Manual that includes a Pollution Prevention 

manual for guidance and examples. \Jon;.~~---

De=ibe the expected net benefit>, •uch., (1) <m ovenill <edu<tion in risk to human h Jl.,~tion in 

the volume manufactured; {3) a reduction in the generation of waste materials through recycling, so . er 

means; (4) a reduction in potential toxicity or human exposure and/or environmental release; (5) an increase in pro 

performance, a decrease in the cost of production and/ or improved operation efficiency of the new chemical substance in 

comparison to existing chemical substances used in similar applications; or (6) the extent to which the new chemical substance 

may be a substitute for an existing substance that poses a greater overall risk to human health or the environment. 

The PMN chemical, HFC-43-10mee, is a replacement for environmental undesirable solvents CFC-113, perfluorocarbons and Methychloroform in many 

cleaning and solvent applications. 

The ODP (Ozone Depletion Potential) of HFC-43-1 Omee is zero and GWP (Global Worming Potentia~ is less than 1/5 that of CFC-113. The atmospheric life ot lhe PMN chemical is about 20 years 

compare to that of Perfluorocarbons of 2D00-3000 years. Also, the PMN chemicals atmospheric life 

is such that is should not contrl>ute to atmospheric pollution by decomposing In lower stratosphere 

and therefor should be exempted from VOC regulations. Thus, the PMN chemical should significantly enhance global environmental quality. 

- The PMN ch~mi~l H~C-43-1 Omee is made from fairly expensive raw materials and fairly proprietary process. We, therefore expect to sell~ [ 

range for a fa1rly h1gh-1n-value y 

uses. The cleaning equipment to be used with this material are expected to be upgraded to 

minimize losses from the equipment. Also, the boiling point of this material is about rc higher than 

CFC-113 which will further enhance containment of this material in the equipment to be used with this chemical. 

Due to 10dvances in process and equipment technol~, and the high cost of raw materials and final product, the manufacturing and cleaning processes will be h~hly controlled, thereby reducing the 

probab1l~1es and magnrtudes of losses from spills a fugrtive emissions. The best current available technology wHI be used to minimize these losses and maximtze recycling and containment. 

D Mark (X) this box if you attach a continuation sheet. 

FORM EPA 7710-25 (Rev. 5-95) Page 11 

17 
p.18 

Part III-- LIST OF ATTACHMENTS 

Attach continuation sheets for sections of the form and test data and other data (including physical/ chemical properties and 

structure/ activity information), and optional information after this page. Clearly identify the attachment and the section of the form to 

which it relates, if appropriate. Number consecutively the pages of the attachments. In the column below, enter the inclusive page 

numbers of each attachment. 

Mark (X) the "Confidential" box next to any attachment name you claim as confidential. Read the Instructions Manual for 

guidance on how to claim any information in an attachment as confidential. You must include with the sanitized copy of the notice 

form a sanitized version of any attachment in which you claim information as confidential. 

Attachment name Attachment Confipage number(s) dential 

~. Material Safety Data Sheet (MSDS) 

2. Physical and Chemical Properties 

3. X 

4. Toxicology Assessment 

5. Environmental Effect Data 

Co, /o'>/1 c_c.(o~~ .s~ '- D(E S S.' ,. eyV) tJ - '- E.E L r;~. 

p.::_ v~~- <.r3 'C ( f>u lt7 h. t- ~.,~ {..) 

... 

D Mark (X) this box if you attach a continuation sheet. Enter the attachment name and number. 

FORM EPA 7710-25 (Rev. 5-95) Page 12 

If 
/~• l 1'. ' .. t : , . '-.. .... :\. .... , i'r 

DuPont 

Material Safety Data Sheet 

p.19 

Page 1 

----------------------------------------------------------------------

05570733 

HFC-43-10MEE 

Revised 4-AUG-1995 

CHEMICAL PRODUCT/COMPANY IDENTIFICATION 

Material Identification 

Corporate MSDS Number 

CAS Number 

Formula 

Molecular Weight 

: DU008057 

138495-42-8 

CF3CHFCHFCF2CF3 

252 

Printed 7-0CT-1996 

CAS Name : Pentane, 1,1,1,2,3,4,4,5,5,5-decafluoro 

Tradenames and Synonyms 

2,3-DIHYDROPERFLUOROPENTANE 

43-10MEE 

Company Identification 

MANUFACTURER/DISTRIBUTOR 

DuPont 

PHONE NUMBERS 

1007 Market Street 

Wilmington, DE 19898 

Product Information 

Transport Emergency 

Medical Emergency 

: 1-800-441-9442 

: CHEMTREC: 1-800-424-9300 

: 1-800-441-3637 

COMPOSITION/INFORMATION ON INGREDIENTS 

Components 

Material 

1,1,1,2,3,4,4,5,5,5-DECAFLUOROPENTANE 

HAZARDS IDENTIFICATION 

Potential Health Effects 

INHALATION 

CAS Number 

138495-42-8 

% 

99 

Gross overexposure may cause: Suffocation, if air is displaced by 

vapors. Based on animal data, repeated or excessive overexposure 

may cause the following effects: Central nervous system 

stimulation with increased activity or sleeplessness. Tremors. 

Convulsions. These effects may be followed by: Central nervous 

system depression with dizziness, confusion, incoordination, 

drowsiness or unconsciousness. 

Potential effects based on data from OTHER FLUOROCARBONS: 

;;g /'I 
05570733 DuPont p.~ge 

Material Safety Data Sheet 

(HAZARDS IDENTIFICATION - Continued) 

Gross overexposure may cause: Irregular heart beat with a strange 

sensation in the chest, "heart thumping", apprehension, 

lightheadedness, feeling of fainting, dizziness, weakness, 

sometimes progressing to loss of consciousness and death. It is 

unlikely that concentrations sufficient to produce irregular 

heartbeat would be achieved from 

1,1,1,2,3,4,4,5,5,5-DECAFLUOROPENTANE without first producing 

other signs of toxicity. 

SKIN CONTACT 

Immediate effects of overexposure may include: Slight irritation 

with itching, redness or swelling. Repeated and/or prolonged 

exposure may cause: Defatting of the skin with itching, redness or 

rash. Data to evaluate the skin permeation hazard of this 

compound are insufficient. There are no reports of human 

sensitization. 

EYE CONTACT 

Immediate effects of overexposure may include: Eye irritation with 

tearing, pain or blurred vision. 

INGESTION 

The major ingestion hazard is aspiration (liquid entering the 

lungs during ingestion or vomiting) which may result in "chemical 

pneumonia". Symptoms include coughing, gasping, choking, 

shortness of breath, bluish discoloration of the skin, rapid 

breathing and heart rate, and fever. Pulmonary edema or bleeding, 

drowsiness, confusion, coma and seizures may occur in more serious 

cases. Symptoms may develop immediately or as late as 24 hours 

after exposure, depending on how much chemical entered the lungs. 

ADDITIONAL HEALTH EFFECTS 

Increased susceptibility to the effects of this material may be 

observed in persons with pre-existing disease of the: central 

nervous system, cardiovascular system. 

Carcinogenicity Information 

None of the components present in this material at concentrations 

equal to or greater than 0.1% are listed by IARC, NTP, OSHA or ACGIH 

as a carcinogen. 

ltf 

2 
05570733 DuPont p. mage 3 

Material Safety Data Sheet 

---------------------------------------------------------------------- FIRST AID MEASURES 

---------------------------------------------------------------------- First Aid 

INHALATION 

If inhaled, immediately remove to fresh air. Keep person calm. 

If not breathing, give artificial respiration. If breathing is 

difficult, give oxygen. Call a physician. 

SKIN CONTACT 

Flush skin with water after contact. Wash contaminated clothing 

before reuse. 

EYE CONTACT 

In case of contact, immediately flush eyes with plenty of water 

for at least 15 minutes. Call a physician. 

INGESTION 

If swallowed, do not induce vomiting. Immediately give 2 glasses 

of water. Never give anything by mouth to an unconscious person. 

Call a physician. 

Notes to Physicians 

Activated charcoal mixture may be administered. To prepare 

activated charcoal mixture, suspend 50 grams activated charcoal in 

400 mL water and mix thoroughly. Administer 5 mLfkg, or 350 mL 

for an average adult. 

Because of a possible increased risk of eliciting cardiac 

dysrhythmias, catecholamine drugs, such as epinephrine, 

should be used with special caution in situations of 

emergency life support. 

Because of the danger of aspiration, emesis or gastric 

lavage should not be employed unless the risk is justified 

by the presence of additional toxic substances. Activated 

charcoal may induce vomiting, but may be given after emesis 

or lavage to absorb toxic additives. Steroid therapy in 

mild to moderate cases does not improve outcome. Bacterial 

pneumonia often occurs after exposure, but prophylactic 

antibiotics are not indicated and should be reserved for 

documented bacterial pneumonia. 

J~ 
05570733 DuPont 4 

Material Safety Data Sheet 

----------------------------------------------------------------------

FIRE FIGHTING MEASURES 

----------------------------------------------------------------------

Flammable Properties 

Flash Point 

Method 

None 

Tag Open Cup - TOC. 

Fire and Explosion Hazards: 

Use water spray or fog to cool containers. Drums may 

rupture under fire conditions. Decomposition may occur. 

Extinguishing Media 

As appropriate for combustibles in area. 

Fire Fighting Instructions 

Self-contained breathing apparatus (SCBA) is required if 

drums rupture and contents are spilled under fire 

conditions. 

----------------------------------------------------------------------

ACCIDENTAL RELEASE MEASURES 

----~----------------------------------------------------------------- Safeguards (Personnel) 

NOTE: Review FIRE FIGHTING MEASURES and HANDLING (PERSONNEL) 

sections before proceeding with clean-up. Use appropriate 

PERSONAL PROTECTIVE EQUIPMENT during clean-up. 

Evacuate personnel, thoroughly ventilate area, use self-contained 

breathing apparatus. 

Spill Clean Up 

Soak up with sawdust, sand, oil dry or other absorbent material. 

HANDLING AND STORAGE 

Handling (Personnel) 

Avoid breathing vapors or mist. Avoid contact with eyes, skin, or 

clothing. Wash thoroughly after handling. 

Material should not be dispensed from its container by 

pouring, except for small sample containers where fume hoods 

or where other ventilation is used to manage the exposure 

limits. The use of a drum pump is recommended for 

dispensing from shipping containers. 
0 05570733 DuPont 

Material Safety Data Sheet p.~atge 

(HANDLING AND STORAGE - Continued) 

Storage 

Store in clean, dry area. Do not heat above 52 deg c (125 

deg F) as excessive pressure may cause container to leak or 

rupture. 

5 

----------------------------------------------------------------------

EXPOSURE CONTROLS/PERSONAL PROTECTION 

----------------------------------------------------------------------

Engineering Controls 

Normal ventilation for standard manufacturing procedures 

is generally adequate. Local exhaust should be used when 

large amounts are released. Mechanical ventilation 

should be used in low or enclosed places. 

Personal Protective Equipment 

Impervious gloves and chemical splash goggles should be 

used when handling liquid. Under normal manufacturing 

conditions, no respiratory protection is required when 

using this product. Self-contained breathing apparatus 

(SCBA) is required if a large release occurs. 

# Exposure Guidelines 

Exposure Limits 

HFC-43-lOMEE 

PEL (OSHA) 

TLV (ACGIH) 

AEL * (DuPont) 

: None Established 

None Established 

: 200 ppm, 8 & 12 Hr. TWA 

400 ppm, Ceiling 

* AEL is DuPont's Acceptable Exposure Limit. Where governmentally 

imposed occupational exposure limits which are lower than the AEL 

are in effect, such limits shall take precedence. 

----------------------------------------------------------------------

PHYSICAL AND CHEMICAL PROPERTIES 

Physical Data 

Boiling Point 

Vapor Pressure 

Solubility in Water 

pH 

Form 

Color 

Density 

55 C (131 F) 

226 mm Hg @ 25 C (77 F) 

: Insoluble 

: Neutral 

: Liquid 

Clear, colorless 

1.58 gjcm3 @ 25 c (77 F) 

13.2 lb/gal 
05570733 DuPont p.P4lge 6 

Material Safety Data Sheet 

----------------------------------------------------------------------

STABILITY AND REACTIVITY 

----------------------------------------------------------------------

Incompatibility with Other Materials 

Incompatible with alkali or alkaline earth metals - powdered 

Al, Zn, Be, etc. 

Decomposition 

Decomposition: 

Polymerization 

Decomposition products are hazardous. 

HFC-43-10mee can be decomposed by high 

temperatures (open flames, glowing metal 

surfaces, etc.) forming, hydrofluoric acid 

and possibly carbonyl fluoride. 

Polymerization will not occur. 

TOXICOLOGICAL INFORMATION 

Animal Data 

EYE: 

Animal testing indicates this material is a mild eye irritant. 

SKIN: 

LD50: No information found. 

Animal testing indicates this material is a slight skin irritant, 

but not a skin sensitizer. 

INGESTION: 

No information found. 

INHALATION: 

4 hour, ALC, rat: 10,000 ppm (Very low toxicity). 

1,1,1,2,3,4,4,5,5,5-DECAFLUOROPENTANE 5,000 ppm. Single exposure 

caused: Tremors. No cardiac sensitization was observed. 

1,900 - 3,500 ppm Single and repeated exposure caused: the 

following temporary effects - Tremors or convulsions. Behavioral 

effects. Altered clinical chemistry. 

No-observed-Adverse-Effect-Level (NOAEL): 500 ppm 

CARCINOGENIC, DEVELOPMENTAL, REPRODUCTIVE, MUTAGENIC EFFECTS: 
05570733 DuPont 

Material Safety Data Sheet 

p. fSige 

(TOXICOLOGICAL INFORMATION - Continued) 

Animal data show developmental effects only at exposure levels 

producing other toxic effects in the adult animal. No animal data 

are available to define the following effects of this material: 

carcinogenicity, reproductive toxicity. Tests have shown that 

this material does not cause genetic damage in bacterial or 

mammalian cell cultures. It has not produced genetic damage in 

tests on animals. 

7 

-------------------------------------------------~-------------------- DISPOSAL CONSIDERATIONS 

----------------------------------------------------------------------

Waste Disposal 

Reclaim by distillation or remove to a permitted waste 

disposal facility. Comply with Federal, State and local 

regulations. 

----------------------------------------------------------------------

TRANSPORTATION INFORMATION 

----------------------------------------------------------------------

Shipping Information 

DOT/IMO 

Proper Shipping Name : NOT REGULATED BY DOT OR IMO 

----------------------------------------------------------------------

REGULATORY INFORMATION 

----------------------------------------------------------------------

u.s. Federal Regulations 

TSCA Inventory Status : Listed. 

TITLE III HAZARD CLASSIFICATIONS SECTIONS 311, 312 

Acute . 

. Yes 

Chronic . . No 

Fire . . No 

Reactivity No 

Pressure . . No 

LISTS: 

SARA Extremely Hazardous Substance - No 

CERCLA Hazardous Substance - No 

SARA Toxic Chemicals - No 

/df 

J5 
05570733 DuPont 8 

Material Safety Data Sheet 

----------------------------------------------------------------------

OTHER INFORMATION 

NFPA, NPCA-HMIS 

NPCA-HMIS Rating 

Health 

Flammability 

Reactivity 

1 

0 

1 

Personal Protection rating to be supplied by user depending on use 

conditions. 

The data in this Material Safety Data Sheet relates only to the 

specific material designated herein and does not relate to use in 

combination with any other material or in any process. 

Responsibility for MSDS 

Address 

> 

> 

Telephone 

DuPont Chemicals 

Engineering & Product Safety 

: P.O. Box 80709, Chestnut Run 

Wilmington, DE 19880-0709 

(302) 999-4946 

# Indicates updated section. 

End of MSDS 
I 

______________ ...:._ ., ' _ ~ __;_ ; ~c. ______ ...... ,/""'\ ·:::"- _,- __::. ~ ____________ ----tp:r..1!2T-7----

['dYSlCAL A:'~D CHE:\UCAL PROPERTIES WORKSHEET 

r.J t·;:;,;t EPA'; revte'"' of ph:1~1Clllllnd chem•c.ll prop<!!rties J.>t~. plel~ complete the followillg worksheet fur J,ltJ you ?rovide 

~c...! "'dude •t •n the nonce. Identify the property me.uured, the po~ge of the notice on wluch the property .appea~. the v.alue 

,)f the propeny. the units in which the proper1y is me.asured (as necessary) • .and whether or not the property is claimed as 

confidential. You are not re<1uired to submit this worksheet; however. EP . .S.. strongly recommends tl1.at you do so. u it will simplify 

re·11·~·.v .1nd ensure :h.at confidential information is properly protected. You should submit this worksheet .lS a supplement to 

your ;ubmission of test d.lt.J. This worksheet is not a substitute for submo.SSion of test do11.1. 

?rope~/ Mark {X) if ?.lge Value 

provided number 

{a) (b) {c) 

Vapor" pressure 25 ·c X @Temperature 22, Torr 

Density I relative density 25°C X 1.56 g/an3 

Solubility 

@Temperature RT -c X Fully miscible 

Solvent Alcohols, H;tdrocarbons 

g/L 

Solubility in water 0 Tempentuft 29-c X 170 ppm g/1. 

Melting temperature X -· -80 -c 

Boiling/sublimation temperature fl 760 torr pressure X 54°C 

Spectra Infrared X 

. Dissociation constant 

Color 

1'mtde"S'ia"ttts~lt" X Clear, colorless 

Octanol/water partition coefficient 

Henry's Law constant 

Volitalization from water 

Volitalization from soU 

pH @ concentration 

Flammability X Nonflanunable 

Coniidential 

Mark (X) 

(d) 

~abtlity- Form X Liquid at R.T. 

Adsorption/ coefficient 

Other- Specify X 

Critical Temperature X l80.6l°C 

Critical Pressure X 331.9 psia 

Critical Volt.!me X 0.433 liter/mol 

Critical Com~ressibility X 0.263 

l ·; 

I 

' I 

I 

I 

; 

, 
~ont Bus. atns · Confidential lness Informat· ton 

p.28 
T..0."\.1 CObQG Y ASSESS~IENT OF HFC-431 Omce 

DuPont ur:publish~d toxicity studies for HFC-B lOmee are reviewed below, and 

copies of the originJ.l reports are included in Appendi.""< A. 

STJM1,1ARY 

Overall, HFC-4310mee has low toxicity. The compound was only a slight skin 

p.29 

and eye irritant. and had low inhalation toxicity. Signs indicative of neurotoxicity were 

evident in rats during the 90-day inhalation study at exposure concentrations of 2000 and 

3500 ppm; no effects were evident at 500 ppm, the lowest concentration tested. Slight 

decreases in motor activity were evident the day following exposure at 3500 ppm only. 

Slight developmental toxicity was observed in rats at an exposure concentration of 3500 

ppm, and was observed as a decrease in fetal body weights. Maternal effects, body weight 

decreases and convulsions, were also observed at 3500 and 2000 ppm. HFC-4310mee 

was not mutagenic in an Ames assay. and was not clastogenic in an in vivo micronucleus 

study. No cardiac sensitization response was observed in experimental screening studies 

with dogs at 5000 ppm. The occurrence of convulsions at 10,000 ppm. however. 

precluded further cardiac sensitization studies. Based on these studies, DuPont 

established an internal occupation exposure limit (Acceptable Exposure Limit. AEL) of 

200 ppm. 8- and 12-hour TWA. and a 15-minute Short-tenn Exposure Limit of 400 ppm. 

ACUTE TOXICOLOGY S1UD1ES 

HFC-4310mee was evaluated for eye irritation potential in three New Zealand white 

rabbitsO). Approximately 0.1 mL of the material was placed into the conjunctiVal sac of 

each test anima4 and the conjunctiva. iris and cornea subsequently scored for irritation at 

1. 24. 48 and 72 hours post-treatment. The eyes remained unwashed during the study. 

Slight conjunctival redness was observed at 1 and 24 hours post exposure, but all irritation 

had resolved by 48 hours after treatment. 

In a dennal irritation study. 0.5 mL of the test material was applied under an 

occlusive patch to the clipped, intact skin of three New Zealand white rabbits for 4 

hours(2). Dennal irritation was scored at 1, 24, 48, and 72 hours after the end of the 

exposure period. Slight, transient dennal irritation was observed in one rabbit at 24 hours 

after exposure. No irritation was observed in the other animals. 

Seven groups of male Crl:CD®BR rats (n=6/group) were exposed nose-only for 4 

hours to several vapor concentrations of HFC-431 Omee(3). After exposure, the animals 

were observed for 14 days. Mortality occurred in rats exposed to 10,000 ppm and greater 

within one day of exposure (Table 1). Slight to severe body weight losses were observed 

in surviving rats one day after exposure. but all animals gained weight during the recovery 

period. Based on mortality, the approximate lethal concentration CALC) for HFC4310mee was 10.000 ppm. 

1 
TABLE l 

~!ORTALITY IN RATS EXPOSED 4 HOURS TO 

HFC-43l0mee 

5200 

7900 

10,1001 

10,200 

10.5001 

19,400 

67,800 

0/6 

0/6 

3/6 

4/6 

216 

6/6 

516 

p.30 

A group of six beagle dogs was exposed via a face mask to HFC-4310mee 

concentrations of 1000. 5000 or 10.000 ppm(4). About 5 minutes after initiation of 

exposure. the dogs were injected intravenously with a challenge dose of epin~phrine to 

assess the potential for HFC-4310mee to induce a cardiac sensitization response. No 

cardiac sensitization response was observed at either 1000 or 5000 ppm. At 10.000 ppm, 

severe convulsions were observed in one dog which precluded a determination of a 

cardiac sensitization response. The other test dogs and higher exposure concentrations 

were not tested because of the conwlsive response. The conwlsions observed in this 

study were not unexpected based on previous subchronic and rangefmding studies (see 

below). 

SUBCHRONIC TOXICOLOGY STUDIES 

Four groups of 10 male Crl:co®BR rats were exposed nose-only to HFC4310mee concentrations of 0, 500, 1000 or 4000 ppm 6 hours/day. 5 days/week over 12 

days(5). A functional observation battery (FOB) was performed prior to and immediately 

following the third and eighth exposure. After the ninth exposure urine and blood samples 

were collected for clinical pathological evaluations. Following the last exposure the 

animals were sacrificed for gross and microscopic examinations. 

All animals survived the study duration. Significantly lower body weights were 

observed in rats in the 500 and 4000 ppm groups. However, in the absence of a doserelated effect. these body weight changes were not considered toxicologically significant 

Furthermore. a decrease in body weights of animals placed in restraining devices is not an 

unexpected outcome. No compound-related clinical observations were detected, and no 

compound-related effects in the FOB were observed. No compound-related organ weight 

changes or gross or microscopic observations were detected in the animals. Therefore, 

1 Two samples of EFC-4310mee were used in this ALC study, and the composition of the samples differed slightly. 

For these samples (footnoted as 1) the composition was 98.6% pure with 1.3% of a HFC-4310mee isomer. The 

sample used to ge:-:erate the remainder of these data had a purity of 95.6% with 4.2% of a HFC-4310mee isomer. 

2 
untkr tl:e ~uditior".s ~:"·t.'1is study. the no-obser~~ble-ldverse-effect level (NOAEL) for 

this nosl.!-only 2-w~;;:k study was ~000 ppm. 

p.31 

In preparation to initiate the 90-day inhalation study, a series of inhalation rangefmding studies(6) were conducted to select concentrations for the 90-day inhalation study. 

During these range-finding studies, clinical signs of neurotoxicity were observed that 

occurred when a certain chamber concentration was reached (>3500 ppm). However, the 

clinical signs were transient, and were no longer observed approximately 2 hours 

(depending on concentration) after their fli'St occurrence even though the animals 

continued to be exposed to the test material. Following termination of the exposure, some 

animals were evaluated with an abbreviated FOB. However, clinical signs of neurotoxicity 

were not observed after the conclusion of the exposure or prior to a subsequent exposure. 

On subsequent exposure days, the same pattern of clinical signs was repeated with 

exposure to HFC-4310mee. 

In the rangefmding study. 5 male and 5 female rats per concentration were exposed 

four times for 6 hours to nominal concentrations of 4000 or 7000 ppm of the test material. 

The study was conducted in stainless steel chambers with a glass window for gbserving 

the animals. However, because of chamber construction not all of the animals were visible 

at any given time. During exposure, the animals were observed for clinical signs of 

toxicity. After exposure, an abbreviated FOB was conducted. 

The test material provided contained a different concentration of a low boiling 

diastereomer of HFC-431 Omee designated as Isomer A and a higher boiling HFC4310mee diastereomer designated as Isomer B. The fU"St batch of test material used for 

the rangefmding study contained 8.9% of isomer A and 90.8% of isomer B. However, 

because of limited test material, a second batch of material, containing 6.5% of Isomer A 

and 93.3% of Isomer B, was used in the three subsequent exposures. The results of this 

rangefinding study for each exposure are presented below. 

• Exposure #1 (Test Material- 8.95% Isomer A /90.84% Isomer B): 

Two groups of rats were exposed to target concentrations of 4100 and 7000 

ppm, respectively. Within 20-30 minutes after initiation of exposure to 4100 

ppm, animals exhibited clinical signs of jwnping, flipping and had nasal 

discharge. Within 45 minutes after initiation of the exposure, the animals that 

were visible through the chamber window did not respond to a sound stimUlus. 

However, approximately 3 hours after the beginning of the exposure, the animals 

responded normally to a sound stimulus. At the conclusion of the exposure, the 

results of the abbreviated FOB were similar to pre-exposure. 

Animals exposed to a target concentration of 7000 ppm were inadvertently overexposed to a concentration of approximately 11.00) ppm. Clinical signs included 

jumping, convUlsions, gasping, labored breathing and flipping. Mortality 

occurred :n 50% of the animals within 40 minutes. Tne remainder of the 

exposure ~rcceeded with chamber concentrations ranging between 5000 and 

3 
10.000 ppi.. Th~ :lnim:.1Is which survived lh~ ~xp~c .w~re :i:.lcrificed without 

pltholog:.:~ ~v:llu:1tion or a FOB ev:lluation. 

• Exposure ;f2 i_T;!st M:uerial- 6.5% Isomer A I 93.3% Isomer B): 

Prior to the start of the second exposure, the animals previously were evaluated 

by an abbreviated FOB. There were no abnormal clinical signs observed. About 

30 minutes after vapor generation was initiated (at a chamber concentration of 

approximately 4000 ppm). clinical signs of tremors. shaking, flips. and 

convulsions were observed. Within 1 hour after vapor generation was initiated, 

the animals responded normally to a sound stimulus. For the remainder of the 

exposure period (approximately 3 hours due to a power failure), the chamber 

concentntion ranged between 4000-5000 ppm. 

A group of previously unexposed males and females (5/sex) were exposed to a 

target concentration of 7000 ppm. Approximately 15 minutes after initiation of 

exposure, clinical signs of tremors, abnormal gait, twitching, convulsions, and 

low posture were observed when the chamber concentration was app["')x.imately 

4200-5300 ppm. The exposure continued approximately 4 hours and clinical 

signs were observed throughout the exposure period at concentrations ranging 

from 5000-7300 ppm. 

• Exposure #3 (Test Material - 6.5% Isomer A /93.3% Isomer B): 

p.32 

Prior to the start of vapor generation, there were no abnormalities detected in the 

abbreviated FOB conducted on animals receiving two previous exposures to · 

4000-5000 ppm. Within 20 minutes after initiation of vapor generation (at a 

chamber concentration of approximately 3500 ppm), clinical signs of 

convulsions. jerky movements, involuntary muscular reflexes, and discharge from 

the mouth were observed. Approximately 1 hour after initiation of vapor 

generation, the animals responded to a sound stimulus, but were lethargic (at 

chamber concentration of approximately 4,600 ppm). Approximately 2 hours 

after initiation, the animals responded normally to sound, and there were no 

additional clinical signs for the remainder of the exposure (at chamber 

concentrations ranging up to approximately 5,900 ppm). After the conclusion of 

the exposcre, there were no abnormalities detected in an abbreviated FOB. 

Prior to the start of vapor generation, there were no abnormalities detected in an 

abbreviated FOB in animals previously exposed to approximately 7,000 ppm. 

Within 20 minutes after initiation of vapor generation (at a chamber 

concentration of approximately 4,500 ppm) convulsions and seizure-like clinical 

signs were noted. Approximately 1 hour after exposure initiation, the animals 

respondee ~ a sound stimulus. but were lethargic (chamber concentration was 

approxim:1~ly 5,800 ppm). Approximately 2.5 hours after exposure initiation, 

the anirn:Ls responded normally to sound, and there were no additional clinical 

signs fer :.':e remainder of the exposure (at chamber concentrations ranging up to 

4 
appro:tirr...l:.ely 7 .lOO ppm). After the conclusion of the exposure. several 

animals were noted to have discharge from the nose and mouth. 

• Exposure~ CTest Material- 6.5% Isomer A I 93.3% Isomer B): 

p.33 

Prior to the start of vapor generation, no abnormalities were detected in the FOB 

conducted on animals which had three previous exposures to HFC-4310mee. 

Within 1 hour after initiation of vapor generation (at a chamber concentration of 

approximately 4,900 ppm), seizure-like clinical signs were observed. Within 2.5 

hours after vapor initiation (at a chamber concentration of approximately 4,900 

ppm), the animals responded normally to sound and there were no additional 

signs for the remainder of the exposure. After the conclusion of the exposure, 

there were ~o abnonnalities detected in the FOB. 

In a FOB conducted prior to the start of vapor generation on animab which 

received two previous exposures to approximately 7,000 ppm, several animals 

exhibited labored breathing. Within one hour after initiation of vapor generation 

(at a chamber concentration of approximately 5,500 ppm), seizure-like clinical 

signs were observed. Within 2.5 hours after vapor initiation (at a chamber 

concentration of approximately 6,500-7,000 ppm), the animals responded 

normally to sound and there were no additional clinical signs for the remaind.er of 

the exposure. After the conclusion of the exposure, 1 animal was found dead, 

and several animals were noted to have labored breathing. 

Based on the results of this study. it was apparent that the neurotoxicity induced 

by HFC-4310mee was an acute phenomenon and that cumulative toxicity was not evident 

The convulsive response was clearly observed at concentrations of 3500 ppm and greater. 

Because this response was an acute response to the test material, a series of single 

exposure studies were conducted to better define a possible "threshold" for this response 

to ensure adequate dose selection for the 90-day study. Furthermore, the concentrations 

of isomers A and B were modified to determine if the toxicity of the test material also 

changed with the composition. 

• Single Exposure (1.03% isomer A /98.8% isomer B): 

Four male rats per concentration were exposed to nominal concentrations of 

approximately 2,000, 3,000, or 4,000 ppm. Seizure-like behavior occurred at a 

chamber concentration of 3,759 ppm (actual). Clinical signs of toxicity were not 

observed during exposures to nominal concentrations of 3,000 ppm or below. 

• Single Exposure (44.85% Isomer N 53.11% Isomer B): 

Four male rats per concentration were exposed to nominal concentrations. of 

approximately 500 - 5,000 ppm. Seizure-like behavior occurred at a chamber 

concent.'"ation of 4,538 ppm (actual)and above. Clinical signs of toxicity were 

not obse::·ved during exposures to concentrations of 4,200 ppm (actual)or below. 

5 

:1.::1 
• Single Exposure (3.95% isomer A /90.84% isomer B): 

Four male nts were exposed to a nominal concentration of 4,000 ppm. Seizurelike behavior occurred at a chamber concentration of 4,385 ppm (actual). 

p.34 

Based on the results of the rangefinding studies, the 90-day inhalation study was 

conducted at concentrations afO, 500,2000 and 3500 ppm(7). Exposures were 

conducted whole-body for 6 hours/day, 5 days/week for 13 weeks. A FOB and an 

assessment of motor activity (MA) were conducted on 10 rats/group prior to initiation of 

the study, and at weeks 4, 8 and 13. The FOB and MA were conducted on non-exposure 

days about 16 hours following the preceding exposure. Clinical pathology and pathology 

measurements were also conducted. 

No compound-related mortalities were observed. Rats exposed to 2000 and 3500 

ppm exhibited jerking behavior and convulsions during exposure. These Behaviors were 

observed within about 1 hour of exposure initiation. Within about 2 hours of achieving 

the chamber concentration. the animals appeared to adapt and these clinical signs were 

infrequently observed. No changes in FOB or MA were observed at 2000 or 500 ppm. 

At 3500 ppm, a decrease in MA was observed. 

A compound-related decrease in serum albumin and urine osmolality and an 

increase in urine volume were evident in male or female rats exposed to 3500 ppm. The 

biological significance of these fmdings are uncertain since correlative changes in other 

clinical pathology or pathology measures were not evidenl An increase in urinary fluoride 

was observed in rats exposed to 2000 and 3500 ppm. This latter fmding was not 

considered to be adverse, but reflects the possible metabolism of the test material. Also, a 

dose-related decrease in ~-oxidation activity towards 14c-palmitoy1 CoA. a measure of 

peroxisome proliferation. was observed. However. in the absence of changes in other fat 

metabolism parameters, e.g .• cholesterol. triglycerides, this fmding was not considered to 

be adverse. No compound-related gross or microscopic changes were noted in any of the 

treated rats. 

DEVELOPMENTAL TOXICOLOGY 

In a pilot developmental toxicity study conducted in conjunction with the 2-week 

subchronic study (see above), four groups of time-pregnant rats (n=6/group) were 

exposed, nose-only, to HFC-4310mee concentrations of 0, 500, 1000 or 4000 ppm 6 

hours/day during gestation days 7-16(8). No evidence of maternal toxicity was observed 

throughout the study. Also, no changes in pregnancy rate, fetal viability, resorptions or 

nidation were observed. Fmally, no fetal toxicity was observed in any of the exposure 

groups. 

In the main developmental toxicity study, groups of 25 rats were exposed, wholebody, on days 7-16 of gestation to concentrations of 0, 500, 2000 or 3500 ppm (9). A 

dose-related dec:ease in maternal body weight g3.ins and food consumption were observed 

at 200) and 35C{) ppm. Also, as in the 90-day study, convulsions were evident in rats 

6 
p.35 

exposed to 2000 l.."':d 350<) ppm. No evidence of m:.1tem:ll toxicity was observed at 500 

ppm. Signifi.can:.!y reduced fet:ll body weights were observed at 3500 ppm, and were 

indicative of d!!velopmental toxicity. No other developmental toxicity was evident in the 

off-spring. Under the conditions of this study. the NOAEL for maternal effects was 500 

ppm, and NOAEL for fetal effects was 2000 ppm. 

GENOTOXICITY 

HFC-4310mee was evaluated for mutagenicity in S. typhimurium strains T AlOO, 

TA1535, TA97 and TA98, and in E. coli strain WP2uvrA(pKm101) with and without 

metabolic activationClO). The maximum concentration tested was 5000 J.lg/mL. No 

evidence of mutagenicity was observed in any of the tester strains. 

An inhalation micronucleus test was conducted with rats to determine if HFC43l0mee was a potential clastogen01). Rats were exposed, whole-body: on 2 

consecutive days to concentrations of 0. 2000. 3500 or 7000 ppm for 6 hours/day. Bone 

marrow smears were prepared approximately at 24 and 48 hours after the second 

exposure, and 2000 cells per animal evaluated. No significant changes in the number of 

m.icronucleated polychromatic cells were observed at any of the tested concentrations. 

Therefore, HFC-4310mee was not considered to be clastogenic. 

In summary. HFC-4310mee has low acute toxicity potential. During exposure, 

however, clinical signs suggestive of neurotoxicity are observed, and consist of 

convulsions, tremors, jerking behavior. etc. These clinical signs are transient, and there 

appears to be an adaptive response to the exposure, Le., after about 1-2 hours of 

exposure, no clinical signs of neurotoxicity are observed. About 16 hours after exposure, 

reduced motor activity is observed, but only at higher concentrations (3500 ppm). The 

convulsive response observed to HFC-4310mee appears to be an acute response. No 

convulsions are observed after termination of exposure. but on subsequent exposure days, 

the same pattern of clinical signs was repeated during exposure to HFC-4310mee. The 

no-observed-effect level from the subchronic inhalation studies was 500 ppm. The 

material produces slight fetal effects (reduced fetal body weights) but does not induce 

structural changes in off spring. Also, HFC-4310mee was not genotox.ic. Based on these 

studies, DuPont established an internal occupation exposure limit (Acceptable Exposure 

Limit, AEL) of 200 ppm, 8- and 12-hour TV{ A. and a 15-minute Short-term Exposure 

Limit (STEL) of 400 ppm. 

ADDffiONAL STUDIES 

Additional studies are currently planned for HFC-4310mee and include. 

• Derm~ sensitization in guinea pigs 

• In vitro chromosomal aberration study with hwnan lymphocytes 

The re.sults of these studies will be provided when concluded. 

7 
p.36 

An additional series of acute and repeated exposure studies are being conducted 

with the test material to better define the threshold for convulsive effects. During the 90-

day study, an accurate incidence of convulsions at 2000 ppm and above could not be 

determined because. not all of the animals were visible in the chamber at any given time. 

In this additional study, the animals are being exposed in a Plexiglas chamber such that all 

of the animals are visib~ Also, during exposure. a modified FOB is being conducted. 

The results of these studies are expected in early 1995. 

8 
p.37 

REFERENCES 

l. DuPont Company, unpublished data, HLR 386-93, 1993. 

2. DuPont Company, unpublished data, HLR 385-93. 1993. 

3. DuPont Company, unpublished data, ID...R 77-93, 1993. 

4. Huntingdon Research Centre. unpublished data, OPT 304/942241, 1993 

5. DuPont Company, unpublished data, HLR 819-91. 1991. 

6. EPA TSCA 8(e) Submission. 

7. DuPont Company. unpublished data, HLR 24-94, 1994. 

8. DuPont Company, unpublished data, MR. 9146 

9. DuPont Company. unpublished data, HLR 255-94, 1994. 

10. DuPont Company, unpublished data, HLR 189-94, 1994. 

11. DuPont Company, unpublished data, HLR 53-94, 1994. 

9 

:37 
ENVIORNMENTAL EFFECT DATA 

FOR HFC-43-lOMEE 

OZONE DEPLETION POTENTAIL ZERO 

ATMOSPHERIC LIFE 20.8 YEARS 

GLOBAL WARMING POTENTIAL 1100 ( 100 YEARS) 

( COMPARED TO CARBON DIOXIDE) 375 ( 500 YEARS) 

VOLATILE ORGANIC CARBON EXEMPTION TO BE FILED 

(VOC ) 

p.38 
jJ-r:r~CH}AG~( (c 

- -- ·------- . -

Study Title 

Acute Dermal Irritation/Corrosion Study with HFC-43-1 Omee in Rabbits 

Study Title 

Primary Eye Irritation Study with HFC-43-1 Omee in Rabbits 

Study Title 

Mutagenicity Testing ofH-20255 in the Salmonella Typhimurium and 

Escherichia Coli Plate Incorporation Assay 

Study Title ~ 

Rat Bone Marrow Micronucleus Assay of H-20255 By Inhalation 

Study Title 

p.39 

Inhalation Approximate Lethal Concentration (ALC) ofH-18780/H-18891 

in Rats 

,,, ... ' .. . ..,_ t . 

HFC-43-1 O:MEE 

Assessment of Cardiac Sensitisation Potential in Dogs 

Study Title 

Two-Week Inhalation Toxicity Study with HFC-43-1 Omee in Male Rats 

Study Title 

Developmental Toxicity Study ofH-20255 in Rats 

Study Title 

Subchronic Inhalation Toxicity: 90 Day Study with H-20255 Inhalation 

Study in Rates 

(Volumes 1, 2, & 3)