# Patent

## Title
Receptor modulators

## Abstract

Method of identifying a modulator of CD28 comprising comparing a structural model of a candidate modulator with a structural model of CD28 to thereby determine whether the modulator will bind to CD28, wherein the structural model is derived from, or comprises, structural coordinates of a crystal of: (i) CD28, (ii) a fragment of CD28, or (iii) a homologue of (i) or (ii). The crystal of CD28 in a soluble form complexed with the Fab fragment of a mitogenic (superagonistic) antibody has been obtained and used for the determination of the 3D-structure of the receptor. The application also relates to modulators of superagonistic signalling for any receptor of the CD28 family, i.e. to superagonistic antibodies and chimeric proteins thereof, and to the screening of the superagonistic modulators. In the methods of screening, the binding of the candidate modulators to a portion of the receptor proximal to the cell membrane is investigated.

## Background
CROSS-REFERENCE TO RELATED APPLICATION
This application is the U.S. national stage application of International Patent Application No. PCT/GB2005/000099, filed Jan. 10, 2005, which claims the benefit of U.S. Provisional Patent Application No. 60/536,354, filed Jan. 14, 2004, the disclosures of which are hereby incorporated by reference in their entireties, including all figures, tables and amino acid or nucleic acid sequences.
FIELD OF THE INVENTION
The present invention relates to use of a crystal structure to obtain modulators of a cell surface receptor and to the generation of therapeutic antibodies and chimeric proteins that bind a particular class of signalling receptors.
BACKGROUND OF THE INVENTION
CD28 is present on the surface of T cells and plays an important role in their activation. Signal transduction occurs through CD28 after it is activated (triggered) by binding to its ligand. CD28 activation is dependent on phosphorylation of its cytoplasmic domain. CD28 does not have intrinsic phosphorylation activity but instead is dependent on an extrinsic kinase, e.g. p56lck.
SUMMARY OF THE INVENTION
The invention relates to the obtaining of the structure of CD28. This was done by crystallizing a CD28/Fab fragment complex, subjecting it to X-ray diffraction and deriving the structural coordinates from the diffraction measurements. The Fab fragment is from an antibody that has superagonist activity towards CD28, i.e. is able to cause activation of CD28 without the need for a T cell receptor-derived signal. Conventional antibodies that activate CD28 need an additional signal generated by the T cell receptor. The deduced structure allows modulators of CD28 signalling to be obtained which can in turn be used to modulate the immune system.
Accordingly the invention provides a method of identifying a modulator of CD28 comprising comparing a structural model of a candidate modulator with a structural model of CD28 to thereby determine whether the modulator will bind to CD28, wherein the structural model is derived from, or comprises, structural coordinates of a crystal of: (i) CD28, (ii) a fragment of CD28, or (iii) a homologue of (i) or (ii).
In addition the invention relates to antibodies and chimeric proteins that are capable of being superagonists of particular receptors by preferentially excluding phosphatases (as opposed to kinases) from the vicinity of the said receptor.
Accordingly the invention provides an antibody that causes superagonistic signalling of a cell surface receptor, wherein said antibody binds to the extracellular portion of the receptor at a membrane proximal region and said receptor comprises a cytoplasmic domain which is dependent on an extrinsic protein kinase, wherein said antibody does not bind only the C′-D loop of human CD28.
In addition the invention provides a chimeric protein that causes superagonistic signalling of a cell surface receptor, which chimeric protein comprises

    
    
        (i) sequence representing a fragment of a ligand of the receptor, or a homologue of such a fragment, wherein the fragment or homologue is capable of binding to the extracellular portion of the receptor at a membrane proximal region, and
        (ii) an Fc region of an antibody,

wherein said receptor comprises a cytoplasmic domain which is dependent on an extrinsic protein kinase.

    
    


Further the invention provides a chimeric protein that causes superagonistic signalling of a first cell surface receptor, which chimeric protein comprises two Fv regions of an antibody that may be the same or different, wherein at least one of the Fv regions is capable of binding to said first receptor, and the other Fv region binds to a second cell surface receptor expressed on another cell, wherein said first receptor comprises a cytoplasmic domain which is dependent on an extrinsic protein kinase, and the first receptor can be identical to the second receptor.



DESCRIPTION OF THE DRAWINGS
FIG. 1 shows mechanisms of differential triggering of extrinsic kinase-dependent receptors, by superagonistic agents in vitro and in vivo.
A. In vitro superagonistic signalling, giving the example of CD28 antibodies. The basic signalling principle is as follows. Antibodies raised against CD28 bind distally (“conventional” antibodies, left) or membrane-proximally (“superagonists”, right), as indicated by the structure of the CD28-5.11A1 antibody-Fab complex. The antibodies hold the cell surface at certain distances from an immobilising substrate: in this case plastic, and as shown in parts B, C and D of the figure, Fc receptor- or other receptor-bearing cells. For superagonistic antibodies this distance is typically 150-200 Å, whereas for costimulatory antibodies, it is considerably larger.
The induced proximity of the membrane and the immobilising substrate in the region of the immobilised antibody and receptor will lead to the differential steric exclusion, from the immediate vicinity of the receptor, of other molecules whose extracellular domains are comparable in size or larger than CD28-antibody complexes, such as the tyrosine phosphatase, CD45. In contrast, tyrosine kinases, e.g. p56lck, will be unaffected because they are small and/or attached to the inner leaflet of the membrane. The result is that, overall, the phosphorylation of CD28 by the kinases will be favoured over its de-phoshorylation by phosphatases, with the net increase in phosphorylation amounting to receptor triggering. Superagonists are more potent than conventional antibodies because they bind epitopes close to the membrane rather than at the “top” of the molecule, leading to more efficient exclusion of, e.g., CD45, and therefore a larger increase in the net phosphorylation of CD28.
B. In vivo superagonistic signalling, giving the example of CD28 antibodies. The binding of the antibody to the membrane-proximal region of CD28 on a T cell, and to the Fc receptor of, e.g. an antigen presenting cell, forces the membranes of the two cells into close proximity (150-200 Å). This in turn excludes CD45 from the immediate vicinity of CD28 as described in A, leading to signalling by CD28.
C. In vivo superagonistic signalling, giving the example of a chimeric, ligand-based agent. The chimera consists of a receptor-binding region of the ligand of the receptor, fused to the Fc region of an antibody. The binding of the ligand portion of the chimera to the receptor, and of the Fc region of the chimera to the Fc receptor of, e.g. an antigen presenting cell, forces the membranes of the two cells into close proximity (150-200 Å). This in turn excludes CD45 from the immediate vicinity of the receptor as described in A, leading to signalling by the receptor.
D. In vivo superagonistic signalling, giving the example of a chimeric, Fv-based agent. The chimera consists of the receptor-binding Fv region of one antibody, fused to the Fv region of a second antibody reactive with another receptor on a second cell. The binding of the chimera to both receptors forces the membranes of the two cells into close proximity (150-200 Å). This in turn excludes CD45 from the immediate vicinity of the receptor as described in A, leading to signalling by the receptor.
FIG. 2 shows the method for identification of binding sites for superagonist antibodies of receptors raised against example receptors.



DESCRIPTION OF THE SEQUENCES MENTIONED HEREIN
SEQ ID NO:1 shows the amino acid sequence of CD28.
SEQ ID NO:2 shows the sequence of the CD28/Fc fusion protein used to express and dimerise CD28.
DETAILED DESCRIPTION OF THE INVENTION
The CD28 Protein
Many of the different aspects of the invention discussed herein refer to CD28. It is to be understood that references to CD28 herein also include (i) a homologue of CD28, or (ii) a fragment of CD28 or the homologue, or (iii) a fusion protein comprising CD28, (i) or (ii), unless the context requires otherwise. The homologue and/or fragment of CD28 may be of particular lengths, as discussed below, or may have the binding or functional properties of naturally occurring (native) CD28, such as the ability to bind a cell membrane and/or bind to B7-1 or B7-2. The homologue and/or fragment may comprise the extracellular domain of CD28. The homologue and/or fragment may comprise, or essentially consist of, the fragment of CD28 present in the fusion protein of SEQ ID NO:2. The homologue and/or fragment may have the ability to transduce a signal to the cytoplasm of a T cell.
The CD28 may be of any species of animal, such as a mammalian or avian CD28. The CD28 is preferably a human CD28, for example as shown in SEQ ID NO:1. The CD28 protein may be present in particular forms, for example which aid expression and/or crystallization. Thus the CD28 may be fully glycosylated, partially glycosylated or lack glycosylation and/or have a reduced and alkylated stalk region.
The CD28 Crystal
The crystal of CD28 generally comprises CD28 present in a regular repeating array. As mentioned above the term “CD28” includes fragments and/or homologues. Preferred fragments or homologues present in the crystal comprise the extracellular domain of native CD28. In the crystal the CD28 is preferably in the form of a monomer. The crystal may be of CD28 bound to another moiety. Such a moiety may be an antibody specific for CD28, including fragments/derivatives of the antibody (as further discussed below), which bind CD28. In a preferred embodiment the crystal is of CD28 bound to the Fab fragment of an antibody. The crystal may comprise CD28 in a form that aids crystallization, and thus the CD28 may be fully glycosylated, partially glycosylated or lack glycosylation and/or have a reduced and alkylated stalk region. In one embodiment the crystal has the coordinates shown in Table 4.
The crystal is generally obtained by providing a solution that comprises CD28 and optionally a moiety that binds to CD28, such as an antibody fragment, and subjecting the solution to conditions that allow the crystal to form. The CD28 which is to be crystallized is generally obtained by recombinant expression, optionally in the form of a fusion protein. The fusion protein may comprise CD28 and a polypeptide sequence which forms a homodimer. Such a fusion protein aids the formation of a CD28 homodimer. Preferably the fusion protein comprises the sequence of the Fc region of an antibody. The fusion protein may be cleaved before crystallisation to separate CD28 from the other polypeptide sequence, for example by thrombin.
The CD28 may be expressed in any suitable cell that is able to express large amounts of CD28, such as a Chinese hamster ovary (CHO) cell.
The CD28 may be further treated in order to aid crystallization. Binding to an antibody fragment, such as a Fab fragment, may be used to prevent the N-linked glycans present on the fully glycosylated form of CD28 from inhibiting the crystallisation of the protein. In one embodiment the antibody (or fragment thereof) is a superagonistic antibody, which may have any of the properties of the superagonistic antibodies mentioned herein. Thus, in one embodiment the antibody (or fragment thereof) binds to a loop region in the extracellular membrane proximal region of CD28, such as the C′-D loop (said loop being defined for example as defined in US-A1-2003/0166860 as the sequence from amino acid positions 52 to 66 CD28 represented by the sequence GNYSQQLQVYSKTGF).
The treatment may comprise reduction of the interchain disulphide bonds in the stalk-like region of CD28, e.g. using dithioreitol (DTT). The reduced cysteines may then be inactivated, for example by alkylation (typically the alkyl moiety has 2 to 6, preferably 2, carbons). The alkylation may be performed using iodoacetamide.
Crystallisation is typically carried out at 15 to 25° C., such as at 17 to 19° C., preferably 18° C. Magnesium formate and polyethylene glycol (PEG) may be used as precipitating agents. Preferably precipitation is carried out using 0.15 to 0.25 M magnesium formate (such as 0.2 M magnesium formate) and 15 to 25% PEG 3350 (such as 20% PEG 3350).
In the work described in the Examples it was found that in order to crystallise CD28 this protein had to be expressed in the form of a fusion protein with a second protein capable of forming a homodimer (the Fc region of an antibody), the fully glycosylated form of CD28 needed to be complexed with an Fab fragment of an antibody in order that the N-linked glycans did not interfere with crystallisation, and the disulphide bonds in the stalk region of CD28 needed to be reduced and alkylated so that the stalk did not interfere with crystallisation. Thus in a preferred embodiment the method of obtaining a crystal of CD28 (including fragment and/or homologue thereof) comprises

    
    
        (a) expressing CD28 in the form of a fusion protein with a second protein that is able to form a homodimer, wherein the presence of the second protein in the fusion protein causes CD28 to dimerise,
        (b) cleaving the second protein from the fusion protein,
        (c) reducing and alkylating the disulphide bond present in the stalk-like region of CD28, and
        (d) crystallising CD28 bound to the Fab fragment of an antibody.

Identifying a Modulator of CD28

    
    


The invention provides a method of identifying a modulator using the structural coordinates determined from the above-mentioned crystal of CD28. The structural coordinates used in the method may be in the form of a structural model, such as a three dimensional representation of the structure or a pharmacophore. The coordinates/model typically comprise information relating to the identity of each atom (i.e. whether it is nitrogen, oxygen, hydrogen etc.) and its three dimensional location (normally defined by three spatial coordinates) in the structure. The model may also comprise additional information relevant to obtaining modulators, such as the electronic charge at different locations in the structure or information concerning whether or not the bonds in the structure can be rotated.
The coordinates/model used in the method typically comprise a specific region of the surface of CD28 corresponding to the site where it is desired for the modulator to bind. Such a site typically comprises Glu-32, Arg-34, Tyr-51, Glu-97, Met-99, Tyr-100, Pro-101, Pro-102, Pro-103, Tyr-104 and Leu-105, or a part thereof which comprises one or more of these amino acids. Preferred modulators bind to such a site or part thereof. In one embodiment the modulator is able to inhibit binding of another moiety to this site. Such a moiety may be an antibody which is specific to the site.
In the method the coordinates/model of CD28 are compared to the structural coordinates/model of a candidate modulator to determine whether or not the candidate modulator will bind to CD28. The comparison may be performed by any suitable means, such as the methods described or referenced in Lyne (2002) Drug Discovery Today 7, 1047-1055. Thus one or more of the algorithms described in this document may be used, such as one or more of Dock, FlexX, FlexE, Slide, Fred, Gold, Glide, AutoDock, LigandFit, ICM, QXP, Amber, CHARMM, SCORE, VALIDATE, Chemscore, Ludi, PLP, PMF, Bleep, SmoG, ZAP, VIDA, GRID, MCSS, Superstar and ROCS.
The method typically comprises deducing one or more ways of fitting (docking) a candidate modulator with CD28 followed by an evaluation (scoring) of the fit. The evaluation may comprise deducing the binding energy between CD28 and the modulator. This may be done based on the interatomic distances between the atoms involved in binding or by analysis of the force fields of CD28 and the modulator. In one embodiment the evaluation comprises comparing the similarity between the fit between CD28 and the candidate modulator and the fit between one or more other proteins and their ligands. Thus the evaluation may comprise comparison with a database of structures of proteins fitted/bound to ligands.
A candidate modulator which has been selected computationally as discussed above may be physically tested to determine whether or not it is able to bind or modulate CD28. Any suitable binding or activity assay may be used. The binding assay may measure the extent of direct binding between the candidate modulator and CD28 or instead be in the form of a competition assay. The binding assay may comprise/use of any of the following:

    
    
        (a) an assay of binding to CD28 (which may be immobilized), for example in which
        (i) the inhibition of B7-1 binding to the CD28 (a soluble form of B7-1 may be used) in the presence of the candidate modulator is measured,
        (ii) inhibition of the binding of an antibody (that binds to the ligand binding face of CD28) to the CD28 in the presence of the candidate modulator is measured,
        (b) a scintillation proximity assay (SPA) in which (i) or (ii) above are measured,
        (c) an ELISA assay in which (i) or (ii) above are measured.
    
    


The activity assay may test the effect of the candidate modulator on the ability of B7-1 or B7-2 to activate CD28. In such an assay, B7-1 or B7-2 may be present on a natural or artificial antigen-presenting cell and CD28 may be present on a T cell. CD28 activity may be detected by measuring the extent of T cell activation, for example by determining the extent of T cell proliferation (e.g. thymidine incorporation) or gene expression in the T cell (e.g. with microarrays).
The candidate modulator may be tested using the assays described in Green N J, Xiang J, Chen J, Chen L, Davies A M, Erbe D, Tam S, Tobin J F. (2003) Structure-activity studies of a series of dipyrazolo[3,4-b:3′,4′-d]pyridin-3-ones binding to the immune regulatory protein B7.1. Bioorg Med Chem. 11, 2991-3013 or Erbe D V, Wang S, Xing Y, Tobin J F. (2002) Small molecule ligands define a binding site on the immune regulatory protein B7.1. J Biol Chem. 277, 7363-8.
The Antibody and Chimeric Protein of the Invention
The term “antibody” as used herein is understood to also include fragments and derivatives of the antibody which retain binding ability, unless the context requires otherwise. Such fragments/derivatives include Fv, F(ab′) and F(ab′)2 fragments, as well as single chain antibodies, camelid antibodies and similarly acting proteins.
The antibody of the invention may be of any species, such as a mammalian or bird antibody, preferably a rodent (such as mouse) or primate (such as human) antibody. The antibody may be a chimeric antibody, a CDR-grafted antibody or a humanised antibody. The antibody may be monoclonal or polyclonal. The antibody is preferably an IgG antibody.
The invention also provides two types of chimeric protein that bind to a receptor. One type of chimeric protein comprises the sequence of a fragment of a ligand of the receptor, or sequence that is homologous to such a fragment. The sequence is capable of binding to an extracellular region of the receptor, as discussed further below. The chimeric protein also comprises the Fc region of an antibody, such as that of any of the types of antibody discussed above. Where the chimeric protein is administered to an individual the Fc region may be of an antibody of the same species as the individual.
The second type of chimeric protein provided by the invention comprises two Fv regions of an antibody. The Fv regions may be the same or different. One of the Fv regions is capable of binding to the extracellular region of the receptor (the “first” receptor). The other Fv region may bind the extracellular region of a “second” apposing receptor, this being the same type of receptor as the first receptor (with the second Fv binding at a location which is the same or different from the first Fv region) or an entirely different cell surface receptor, for example in the case where the first and second receptors are each expressed on the surfaces of two cells capable of interacting/contacting each other. Such cells may be any of the types of cell mentioned herein, including T cells.
In one embodiment the second Fv region of the second type of chimeric protein binds to a protein expressed on the surface of T cells, such as the T cell receptor, CD2, CD4, CD5, CD8, CD52 or CS1. The second Fv region may bind proteins expressed on the surface of other cells, such as CD48, CD58, CD59, B7-1 or B7-2. The term “receptor” when used in the present context refers to a protein expressed on surface of a cell (which may or may not be one which is capable of signal transduction, for example).
The antibody and chimeric protein of the invention are able to cause superagonistic signalling of a cell surface receptor when they bind to the receptor, typically according to the mechanism illustrated in FIG. 1. Thus the antibody or chimeric protein is able to cause activation of the receptor (i.e. cause the receptor to transduce a signal to the cytoplasm of the cell) without the need for additional costimulus of the receptor or cell. As mentioned above, in one embodiment the second type of chimeric protein comprises Fv regions able to bind to different types of receptors. Such a chimeric protein is preferably able to induce superagonistic signalling by both of the receptors which it binds.
The antibody and both types of chimeric protein bind the extracellular portion of the receptor at a membrane proximal region of the receptor, typically to a region of the receptor which is within 75 Å of the cell membrane, such as within 60 Å, 50 Å or 40 Å of the cell membrane. However the second type of chimeric protein of the invention typically also binds the extracellular portion of the second apposing receptor (as defined above), within 75 Å of the cell membrane, such as within 60 Å, 50 Å or 40 Å of the cell membrane.
Generally the antibody or chimeric protein will be capable of binding the native form of the receptor (at the extracellular regions discussed above) when the receptor is present on the surface of the cell where it occurs naturally.
The antibody or chimeric protein cause superagonistic signalling by sterically hindering the access of phosphatases (which tend to be large proteins), such as CD45, to the receptor (i.e. hindering contact of the phosphatase and receptor). In the case of the antibody or first type of chimeric protein such steric hindrance may be caused by the antibody binding to the receptor at one end (through the Fab of the antibody of the invention or the ligand sequence of the first type of chimeric protein) and typically binding to a protein on the surface of another cell (e.g. due to the Fc region of the antibody or chimeric protein binding to an Fc receptor expressed on a second cell), thus bringing the cell membrane of the two cells into close proximity in the region of the receptor.
For the second type of chimeric protein, one of the Fv fragments of the chimeric protein will bind to the receptor, and in the case where the other Fv fragment is specific for a second type of receptor expressed on another cell, the other Fv fragment will bind to the second receptor and thus bring the cell membranes of the two cells into close proximity in the region of the first receptor.
For the antibody and both types of chimeric protein, the close proximities of the cell membranes in the region of the first receptor will sterically hinder phosphatases from accessing the receptor, leading to signal transduction or “triggering” of the receptor. The antibody or chimeric protein are typically capable of bringing the cell membranes of two cells within 200 Å of each other, such as within 180 Å, 150 Å or 120 Å of each other.
The antibody or chimeric protein will preferably bind orthogonally to the main axis of the domain of the receptor which is bound, such as at more than 60 degrees from the main axis of the bound domain of the receptor, for example at more than 70, 80 or 90 degrees. The antibody or chimeric protein will preferably lie substantially parallel to the cell surface when bound to the receptor (to ensure the membranes are brought into close proximity).
The antibody or chimeric protein will bind to an extracellular region of the receptor, generally to amino acids of the receptor that are exposed at the surface of the protein. The amino acids in the receptor that are bound may be present in a loop region or a β-strand of the receptor. Preferred loops and strand sequences are shown for key examples in Table 1. The antibody or chimeric protein may bind to any of the epitopes or part thereof shown in Table 1 or an equivalent homologous sequence from the membrane proximal region of other receptors.
The antibody of the invention does not bind only to the C′-D loop of CD28, and thus in one embodiment will bind both the C′-D loop of CD28 and another region of CD28. The antibody may be one which does not bind any portion of the C′-D loop of CD28. The antibody may or may not bind to the C′-D loop (or the equivalent loop) of any other member of the CD28 family of proteins. The antibody of the invention may or may not bind to any or all of the sequences shown in Table 3.
The first type of chimeric protein of the invention may or may not bind to only the C′-D loop of CD28. The first type of chimeric protein may be one which does not bind to any portion of the C′-D loop of CD28. The first type of chimeric protein may or may not bind to the C′-D loop (or the equivalent loop) of any other member of the CD28 family of proteins. The first type of chimeric protein may or may not bind to any or all of the sequences shown in Table 3.
The second type of chimeric protein of the invention may or may not bind to only the C′-D loop of CD28. The second type of chimeric protein may be one which does not bind to any portion of the C′-D loop of CD28. The second type of chimeric protein may or may not bind to the C′-D loop (or the equivalent loop) of any other member of the CD28 family of proteins. The second type of protein may or may not bind to any or all of the sequences shown in Table 3.
Receptors Bound by the Antibody and Chimeric Protein
The receptors which are bound by the antibody or chimeric protein of the invention are expressed on the cell surface. The receptor is capable of being phosphorylated (typically at one or more tyrosine residues in the cytoplasmic region of the receptor), and phosphorylation of the receptor will typically lead to its activation. The receptor will comprise a cytoplasmic domain that is dependent on extrinsic protein kinases to be phosphorylated. Thus the receptor will not have an intrinsic enzymatic (kinase or phosphatase) activity. The receptor will typically comprise tyrosine-containing, activating ITAM motifs (Yxx/Ix7-12YxxL/I), inhibitory ITIM motifs (I/V/L/SxYxxL/V) or “switch” (TxYxxV/I; activating and inhibitory) signalling motifs (where x is any amino acid). These motifs are phosphorylated by cytoplasmic tyrosine kinases, such as the Src kinases, in competition with antagonistic tyrosine phosphatases, such as CD45. The signalling character of the receptors is defined exclusively by the nature of these motifs (ITAM vs ITIM: activating vs inhibitory).
The receptor may be a member of any surface protein superfamily, but is typically a member of the immunoglobulin superfamily. The receptor may be a member of the CD28 superfamily. The receptor may be any of the specific receptors which are shown in Table 1 or 2 or may comprise a sequence which is homologous to the sequence of any of these specific receptors. The receptor may be CD28, CTLA-4, ICOS, PD-1 or BTLA or comprise a sequence which is homologous to the sequence of any of these specific receptors.
The receptor may be of any of the species that are mentioned herein, and thus may be a mammalian or avian, preferably rodent (such as mouse or rat) or primate (such as human) receptor.
The receptor may be naturally present on a cell of the immune system such as a T cell, B cell, myeloid cell, mast cell, NK cell or a granulocyte.
Screening Methods of the Invention
The invention provides a method of obtaining an agent which is capable of superagonising the above-mentioned receptor which binds to the antibody and chimeric protein of the invention. The method comprises determining whether a candidate agent has any of the properties discussed above possessed by the antibody or chimeric protein of the invention which allows them to induce superagonistic signalling by a receptor. Thus the method will generally comprise determining whether a candidate agent is able to bind to an extracellular membrane proximal region of the receptor, and may also determine whether a candidate agent is able to bind
at a particular location from the cell membrane (such as within 75 Å of the cell membrane),
to particular sequences (such as the sequences mentioned in Table 1), or
in a manner (such as orthogonal to the main axis of the bound receptor domain) which is discussed above for binding of the antibody or chimeric protein of the invention.
Suitable extracellular membrane proximal regions of a receptor may be identified using a sequence database search algorithm (e.g. BLAST) to search for the solved structure most related to the receptor in the protein structural database (PDB). This structure would then be used to identify membrane proximal regions (preferably β-strands) in the receptor by sequence alignment. Whether or not a candidate agent binds to the identified sequences could be determined by taking the midpoint of the strand and the side chains of amino acids two- and three-residues below this mid-point could then be mutated. Mutant forms of the receptor mutated at each of these residues would then be expressed (e.g. on suitable target cells that can be transfected stably or transiently, such as 293T cells), and used to screen for candidate agents that bound the non-mutated receptor but failed to bind the mutant receptor, for example using fluorescence-activated cell sorting. It is envisaged that only every second strand in a conventional beta barrel protein would need to be mutated. In the case of a receptor which is a member of the immunoglobulin superfamily, this corresponds to 8-10 mutant lines being generated, in total. Examples of this process are shown in FIG. 2 for the immediate CD28 family members (ICOS and CTLA-4) and for the PD-1 protein, which is representative of receptors not related to CD28.
Agents that induce superagonistic signalling by a receptor may also be identified using a peptide which comprises sequence from the extracellular membrane proximal region of a receptor or homologous sequence thereto. Such a sequence is typically 5 to 20 amino acids long, such as 10 to 15 amino acids long. In one embodiment the agent is identified using peptide arrays. The mapping of discontinuous epitopes can be performed using overlapping peptides derived from the entire primary sequence of a protein. The whole protein sequence is generated in the form of short overlapping peptides (for example each shifted by 3 amino acids), e.g. prepared by standard spot synthesis (Automated Spot Synthesiser, Abimed, Langenfeld, Germany) on Whatman 50 filter paper (Whatman, Maidstone, England). The C-termini of the peptides may be attached to cellulose via a (β-Ala)2 spacer and the N-termini acetylated. The arrays may be subsequently probed either directly on the peptide membrane or after transfer to another surface (e.g. nitrocellulose) using the monoclonal antibody and a chemiluminescence-based detection system (ECL Western Blotting Detection System, Amersham Pharmacia Biotech).
Suitable candidate agents that can be tested in the above screening methods include antibody agents (for example, monoclonal and polyclonal antibodies, single chain antibodies, chimeric antibodies and CDR-grafted antibodies). Furthermore, combinatorial libraries, defined chemical identities, peptide and peptide mimetics, oligonucleotides and natural agent libraries, such as display libraries (e.g. phage display libraries) may also be tested. The candidate agents may be chemical compounds, which are typically derived from synthesis around small molecules. Batches of the candidate agents may be used in an initial screen of, for example, ten substances per assay, and the agents of batches which show the required property tested individually.
Obtaining a Superagonistic Antibody
The invention provides methods for obtaining a superagonistic antibody. In one embodiment the method comprises screening antibodies for the ability to superagonise a receptor, wherein the antibodies have been obtained either by immunizing an animal with the receptor or a homologue or fragment of the receptor, or from a combinatorial antibody library. Preferably the animal is immunized with a protein which comprises the sequences shown in Table 1, or a fragment thereof, or a homologue of such a sequence or fragment.
Whether or not an antibody is a superagonistic antibody may be ascertained by determining whether or not the antibody can bind an extracellular membrane proximal region of a receptor, for example using any of the suitable methods described above in the section of screening for superagonistic agents.
Alternatively the antibody can be tested in an activity assay to see whether or not it causes activation of the receptor. Receptors that are activated by the antibody will either transduce an activating signal or an inhibitory signal to the cell cytoplasm. In the case of receptors which transduce an activating signal, as in the case of CD28, the activity which is tested may be activation of the cell. This can be determined using functional screens based on, for example, cell proliferation, increased cellular calcium, enhanced tyrosine phosphorylation of proteins in the cell or the production of/release of substances by the cell, e.g. IL-2 production: In the case of receptors that transduce inhibitory signals when activated, activation of such receptors can be ascertained by detection of any of the changes which occur to the receptor or the cell when such a signal is transduced, such as changes in the extent of phosphorylation of one or more proteins in the cell.
The binding or activity assay may be carried out in vitro (inside or outside a cell) or in vivo.
In one embodiment of the method the antibody is obtained by immunising an animal with a peptide comprising a sequence of length 5 to 20 amino acids which represents an extracellular membrane proximal region of the receptor, or a homologue of such sequence, and obtaining the antibody produced by the animal against said sequence. Alternatively the antibody may be obtained by selecting an antibody from a combinatorial library based on its ability to bind a peptide comprising a sequence of length 5 to 20 amino acids which represents an extracellular membrane proximal region of the receptor, or a homologue of such sequence.
In one embodiment the peptide which is used to immunise the animal or select an antibody from a library only comprises sequence from the receptor, or a homologue thereof, and does not comprise any additional sequence. In this embodiment the peptide will only be 5 to 20 amino acids long. However in other embodiments the peptide comprises other sequence to the N-terminus and/or C-terminus of the receptor sequence, such as sequence which is different from (and typically not homologous to) receptor sequence.
Antibodies
Various types of antibodies are mentioned herein, including antibodies obtained by immunisation. Such antibodies may be raised against specific epitopes/sequences. An antibody, or other compound, “specifically binds” to a sequence when it binds with preferential or high affinity to the sequence for which it is specific but does not bind or binds with only low affinity to other sequences. A variety of protocols for competitive binding or immunoradiometric assays to determine the specific binding capability of an antibody are well known in the art (see for example Maddox et al, J. Exp. Med. 158, 1211-1226, 1993). Such immunoassays typically involve the formation of complexes between the specific sequence and its antibody and the measurement of complex formation.
Means for preparing and characterising antibodies are well known in the art, see for example Harlow and Lane (1988) “Antibodies: A Laboratory Manual”, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. For example, an antibody may be produced by raising antibody in a host animal.
A method for producing a polyclonal antibody comprises immunising a suitable host animal, for example an experimental animal, with the immunogen and isolating immunoglobulins from the animal's serum. The animal may therefore be inoculated with the immunogen, blood subsequently removed from the animal and the IgG fraction purified.
A method for producing a monoclonal antibody comprises immortalising cells which produce the desired antibody. Hybridoma cells may be produced by fusing spleen cells from an inoculated experimental animal with tumour cells (Kohler and Milstein (1975) Nature 256, 495-497).
An immortalized cell producing the desired antibody may be selected by a conventional procedure. The hybridomas may be grown in culture or injected intraperitoneally for formation of ascites fluid or into the blood stream of an allogenic host or immunocompromised host. Human antibody may be prepared by in vitro immunisation of human lymphocytes, followed by transformation of the lymphocytes with Epstein-Barr virus.
For the production of both monoclonal and polyclonal antibodies, the experimental animal is suitably a goat, rabbit, rat, mouse, guinea pig, chicken, sheep or horse. If desired, the immunogen may be administered as a conjugate in which the immunogen is coupled, for example via a side chain of one of the amino acid residues, to a suitable carrier. The carrier molecule is typically a physiologically acceptable carrier. The antibody obtained may be isolated and, if desired, purified.
The Peptide of the Invention
The invention also provides a peptide of length 5 to 20 amino acids comprising a sequence which binds to the superagonistic antibody of the invention. The peptide may have a length of 10 to 15 amino acids. The peptide may comprise sequence from an extracellular membrane proximal region of any of the receptors mentioned herein or have a homologous sequence thereto (such sequence may have a length of 5 to 20 amino acids). The peptide may thus comprise any of the sequences shown in Table 1 or homologues of such sequences.
Therapeutic Aspects of the Invention
The invention provides substances which can be used to treat a patient. The patient is typically a human or animal, such as a mammal or bird, for example a cow, sheep, goat, pig, camel, horse, dog, cat, goose, duck or chicken.
The modulator of CD28 which is identified from a structural model or structural coordinates of CD28 can be used to modulate the immune system of a patient. Such modulators will either agonise or antagonize CD28 (i.e. cause or contribute to an increase or decrease, respectively, in CD28 signalling activity). Modulators which agonise CD28 may be used to stimulate the activity of T cells (which express CD28) and thus to stimulate an immune response against an antigen. Such modulators may therefore be used in the prevention or treatment of a disease caused by a pathogenic agent, such as a virus, microorganism (for example a bacterium) or multicellular organism; or in the prevention or treatment of a cancer.
Modulators which antagonise CD28 may be used to inhibit the activity of T cells, and thus to inhibit an immune response to an antigen. Such modulators may be used to prevent or treat an autoimmune disease (such as rheumatoid arthritis or asthma), allergy or transplantation rejection.
The superagonistic antibodies, chimeric proteins and agent described herein may also be used in the therapy of patients to prevent or treat a disease. These substances may be used to modulate the state of a cell on which the relevant receptor is present. Thus the substance may activate or inhibit cell activity depending on whether the activated receptor transduces a stimulatory or inhibitory signal to the cytoplasm of the cell. The modulation of the cell may thus be used to treat a disease caused by the cell or a disease which can be alleviated or prevented by the cell.
In a preferred embodiment the cell is a cell of the immune system (e.g. any such cell mentioned herein) and therefore superagonistic antibodies, chimeric proteins and agents may be used to modulate the immune response of a patient. Superagonistic antibodies, chimeric proteins and agents which stimulate an immune response may be used in the prevention or treatment of a disease caused by a pathogenic agent, such as a virus, microorganism (for example a bacterium) or multicellular organism; or in the prevention or treatment of a cancer. Superagonistic antibodies, chimeric proteins and agents which inhibit an immune response may be used to prevent or treat an autoimmune disease (such as rheumatoid arthritis or asthma), allergy or transplantation rejection.
The patient may also be treated by generating a superagonistic antibody response in the patient by immunisation with a peptide that stimulates the generation of such a response. The antibody that is generated will be specific to a sequence present in the peptide. The peptide will comprise sequence from the extracellular membrane proximal region of a receptor, or sequence which is homologous thereto. Such receptor sequence may be any of the membrane proximal sequences of a receptor (or homologues thereof) mentioned herein. Thus the peptide may be any of the peptides mentioned herein which comprise such sequence.
In one embodiment a nucleic acid capable of expressing any of the above-mentioned therapeutic substances is administered to the patient. Such a nucleic acid typically comprises a region which encodes the therapeutic substance and a control region which causes expression of the coding sequence, such as a promoter. Thus the nucleic acid may be in the form of a vector.
The Substances Mentioned Herein
The modulators, antibodies, chimeric proteins, peptides and nucleic acids mentioned herein may be present in a substantially isolated form. They may be mixed with carriers or diluents which will not interfere with their intended use and still be regarded as substantially isolated. They may also be in a substantially purified form, in which case they will generally comprise at least 90%, e.g. at least 95%, 98% or 99%, of the proteins nucleic acids or dry mass of the preparation.
Homologues
Homologues of protein sequences are referred to herein. Such homologues typically have at least 50% homology, preferably at least 60%, 70%, 80, 90%, 95%, 97% or 99% homology, for example over a region of at least 15, 20, 30, 100 more contiguous amino acids. The homology may be calculated on the basis of amino acid identity (sometimes referred to as “hard homology”).
For example the UWGCG Package provides the BESTFIT program which can be used to calculate homology (for example used on its default settings) (Devereux et al (1984) Nucleic Acids Research 12, p 387-395). The PILEUP and BLAST algorithms can be used to calculate homology or line up sequences (such as identifying equivalent or corresponding sequences (typically on their default settings), for example as described in Altschul S. F. (1993) J Mol Evol 36:290-300; Altschul, S, F et al (1990) J Mol Biol 215:403-10.
Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (http://www.ncbi.nhn.nih.gov/). This algorithm involves first identifying high scoring sequence pair (HSPs) by identifying short words of length W in the query sequence that either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighbourhood word score threshold (Altschul et al, supra). These initial neighbourhood word hits act as seeds for initiating searches to find HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Extensions for the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T and X determine the sensitivity and speed of the alignment. The BLAST program uses as defaults a word length (W) of 11, the BLOSUM62 scoring matrix (see Henikoff and Henikoff (1992) Proc. Natl. Acad. Sci. USA 89: 10915-10919) alignments (B) of 50, expectation (E) of 10, M=5, N=4, and a comparison of both strands.
The BLAST algorithm performs a statistical analysis of the similarity between two sequences; see e.g., Karlin and Altschul (1993) Proc. Natl. Acad. Sci., USA 90: 5873-5787. One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two amino acid sequences would occur by chance. For example, a sequence is considered similar to another sequence if the smallest sum probability in comparison of the first sequence to the second sequence is less than about 1, preferably less than about 0.1, more preferably less than about 0.01, and most preferably less than about 0.001.
The homologous sequence typically differs by at least 1, 2, 5, 10, 20 or more mutations (each of which may be a substitution, deletion or insertion of an amino acid). These mutations may be measured across any of the regions mentioned above in relation to calculating homology. The substitutions are preferably conservative substitutions. These are defined according to the following Table. Amino acids in the same block in the second column and preferably in the same line in the third column may be substituted for each other:

















ALIPHATIC
Non-polar
G A P





I L V




Polar - uncharged
C S T M





N Q




Polar - charged
D E





K R



AROMATIC

H F W Y










Administration

The formulation of any of the therapeutic substances mentioned herein will depend upon factors such as the nature of the substance and the condition to be treated. Any such substance may be administered in a variety of dosage forms. It may be administered orally (e.g. as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules), parenterally, subcutaneously, intravenously, intramuscularly, intrasternally, transdermally or by infusion techniques. The substance may also be administered as suppositories. A physician will be able to determine the required route of administration for each particular patient.
Typically the substance is formulated for use with a pharmaceutically acceptable carrier or diluent. The pharmaceutical carrier or diluent may be, for example, an isotonic solution. For example, solid oral forms may contain, together with the active compound, diluents, e.g. lactose, dextrose, saccharose, cellulose, corn starch or potato starch; lubricants, e.g. silica, talc, stearic acid, magnesium or calcium stearate, and/or polyethylene glycols; binding agents; e.g. starches, arabic gums, gelatin, methylcellulose, carboxymethylcellulose or polyvinyl pyrrolidone; disaggregating agents, e.g. starch, alginic acid, alginates or sodium starch glycolate; effervescing mixtures; dyestuffs; sweeteners; wetting agents, such as lecithin, polysorbates, laurylsulphates; and, in general, non-toxic and pharmacologically inactive substances used in pharmaceutical formulations. Such pharmaceutical preparations may be manufactured in a known manner, for example, by means of mixing, granulating, tabletting, sugar-coating, or film coating processes.
Liquid dispersions for oral administration may be syrups, emulsions and suspensions. The syrups may contain as carriers, for example, saccharose or saccharose with glycerine and/or mannitol and/or sorbitol.
Suspensions and emulsions may contain as carrier, for example a natural gum, agar, sodium alginate, pectin, methylcellulose, carboxymethylcellulose, or polyvinyl alcohol. The suspensions or solutions for intramuscular injections may contain, together with the active compound, a pharmaceutically acceptable carrier, e.g. sterile water, olive oil, ethyl oleate, glycols, e.g. propylene glycol, and if desired, a suitable amount of lidocaine hydrochloride.
Solutions for intravenous or infusions may contain as carrier, for example, sterile water or preferably they may be in the form of sterile, aqueous, isotonic saline solutions.
A therapeutically effective amount of substance is administered. The dose may be determined according to various parameters, especially according to the substance used; the age, weight and condition of the patient to be treated; the route of administration; and the required regimen. Again, a physician will be able to determine the required route of administration and dosage for any particular patient A typical daily dose is from about 0.1 to 50 mg per kg, preferably from about 1.0 mg per kg to 10 mg per kg of body weight, according to the activity of the specific therapeutic substance, the age, weight and condition of the subject to be treated, the type and severity of the disease and the frequency and route of administration. Preferably, daily dosage levels are from 5 mg to 2 g.
The following Examples illustrate the invention:
EXAMPLES
Overview of Crystallisation Strategy
In order to undertake crystallization trials with glycoproteins, such as the CD28 homodimer, it is generally necessary to produce large amounts of protein (>10 milligrams). For this the glutamine synthetase-based Chinese hamster ovary cell expression system (Lonza Biologics Plc, UK) was chosen, which is one of the few eukaryotic expression systems capable of glycoprotein production at this level. The Lec3.2.8.1 cell line was used as the expression host as this provides the option of enzymatically removing unnecessary glycosylation after protein folding and secretion has taken place; which generally favours crystallization. In order to enhance homodimerization, the protein was expressed in the form of a fusion protein with the Fc region of immunoglobulin, which is itself a homodimer. Because the link between the CD28 portion and the Fc is extremely flexible (which will generally discourage crystallization), the construct was prepared in a way that made it thrombin-cleavable.
It was found that producing active, thrombin-cleavable protein depended on the location of the cleavage site. When the cleavage site was too close to the ligand binding domain (to make a more compact protein for crystallization), the protein produced was mis-folded.
In general removal of the N-linked glycans from a glycoprotein substantially enhances its ability to crystallise. Unexpectedly, and in marked contrast to other cell surface glycoproteins, which are generally very stable and active after deglycosylation, the CD28 homodimer proved to be very unstable after deglycosylation with the enzyme endoglycosidase H at the slightly acidic pH at which this enzyme is active. Therefore it was necessary to leave the glycosylation of CD28 intact. The glycosylated form of the homodimer failed to crystallise in more than 100 trials.
To reduce the impact of the glycosylation, therefore, an alternative strategy was employed, in which Fab fragments of anti-CD28 antibodies were prepared and crystallised with CD28. Fab fragments are almost invariably unglycosylated, so the formation of complexes with the Fab can be expected to reduce the impact of the glycans by 80%, regardless of where they bind the protein of interest (a Fab is four times the size of the CD28 monomer). The likelihood that the complex of the Fab and protein of interest will crystallise depends on the overall shape of the complex, (i.e. its “compactness”) and on the chemical properties of the surfaces of the complex involved in forming crystal lattice contacts. Because the surface of the complex that will form lattice contacts cannot be predicted a priori, it is impossible to predict whether a given Fab will give crystals when complexed with the protein. Not unexpectedly, therefore, the homodimer still failed to crystallize after being complexed with one Fab molecule per homodimer for two different antibodies (5.11A1 and 9D7), or two Fabs per homodimer for one of these antibodies (5.11A1), in >100 trials per complex.
In addition the stalk region was expected to create problems. The key to obtaining crystals was to prepare monomeric CD28 by gentle reduction and alkylation of the interchain disulphide bond in the stalk-like region of the homodimer. The expectation was that the presence of an intact disulphide in the stalk might rigidify the stalk making the overall structure less compact. Two complexes formed with the monomer and two different Fabs (5.11A1 and 9D7) gave crystals, but only one of these crystal forms (obtained with 5.11A1 Fab fragments) proved to be suitable for data collection. Other antibodies may also have been useful in crystallising CD28, including antibodies that bind to the same loop of CD28 as bound by 5.11A1 (the C′-D loop) or that bind to the ligand binding site of CD28. Suitable antibodies are described in US-A1-2003/0166860 and Luhder et al (2003) J. Exp. Med., 197, 955-66.
Preparation of CD28 Homodimer
(i) Construct Generation
The polymerase chain reaction (PCR) was used to amplify complementary DNA (cDNA) encoding the signal peptide sequence of mouse immunoglobulin heavy chain and the extracellular domain of CD28 (residues 1-134 of the mature polypeptide; SEQ ID NO:1) from 44 ng of the plasmid, pKGe1145. The oligonucleotides used in the reaction added, at the 5′ end, an Xba I cleavage site and 24 nt of the rat CD4 5′ untranslated region and, at the 3′ end, codons for the thrombin cleavage site, LVPRGS. The sequences of the oligonucleotide primers used for this were (oligonucleotide sequences are given in 5′ to 3′ direction) CD28T—5′: TAG TAG TCT AGA CCC CAT CCG CTC AAG CAG GCC ACC ATG GAT TGG CTG CGG AAC TTG; and CD28T—3′: CTA CCA CTA CCC CTG GGT ACC AGG GGC TTA G. In a second reaction, cDNA encoding the heavy chain constant (C) region-2 and C region-3 domains of murine Ig (residues 103-323 of the secreted protein) was amplified by PCR from 44 ng of pKGe1145. At the 3′ end, the oligonucleotides added an Xba I restriction site to aid cloning. The NH2-terminus of the protein encoded by the Ig cDNA had the sequence GSKPSIS rather than GCKPCIC. The sequences of the oligonucleotide primers for this reaction were Ig—5′: CTA AGC CCC TGG TAC CCA GGG GTA GTG GTA G; and Ig—3′: CTA CTA TCT AGA TTA TTT ACC AGG AGA GTG GGA G. The PCR conditions for both reactions were: denaturation at 94° C. for 15 s, annealing at 59° C. for 30 s, extension at 68° C. for 210 s; 2.5 U of Accuzyme polymerase (Bioline Ltd, U.K.), 300 nM oligonucleotides and 200 μM dNTPs were used and the reactions run for 20 cycles.
In a third reaction, PCR was used to anneal the two initial products together to generate cDNA encoding a chimera consisting of the CD28 extracellular domain fused to murine IgGFc via a thrombin cleavable sequence (called the CD28TFc chimera; SEQ ID NO:2). Ten microlitres of each of the initial PCR reaction product mixtures was used as template and 300 nM of the CD28—5′ and Ig—3′ oligonucleotides was also added to the mix. The PCR conditions were otherwise identical to the first set except that they were run for only 4 cycles. The PCR product was gel-purified, cut with Xba I and cloned into the Xba I site of pEE14 for expression in the glutamine synthetase-based gene expression system (Lonza Biologics Plc, UK). The plasmid construct was then sequenced using dideoxy sequencing.
(ii) Expression of the CD28TFc Chimera
Lec3.2.8.1 cells, 2×106/flask, were transfected with 20 μg of DNA/flask for 3 h using Pfx-8 lipids (Invitrogen Inc), and then the cells were cloned the following day by plating out at 2×106 cells/96-well plate. After 2 weeks, the clones expressing the highest amounts of CD28TFc were selected by Western-blotting using the semi-quantitive ECL detection system (Amersham-Pharmacia, UK) and an anti-mouse Fc primary antibody (Sigma-Aldrich Co.). The best-expressing clone was expanded and grown to confluence in bulk culture in Cell Factories (Nunc A/S, Denmark) containing immunoglobulin-free medium (Invitrogen-Gibco Ltd, UK) in the presence of 2 mM sodium butyrate. The supernatant was harvested after approximately four weeks and clarified by centrifugation at 5,000 g.
(iii) CD28TFc Purification
The spent, clarified medium was concentrated to approximately 1⁄6 of the original volume using a Masterflex L/S concentrator (Helixx Technologies, Inc.). The sample was then buffer-exchanged against the original volume of 3 M NaCl, 1.5 M glycine, pH 8.5 high-salt buffer and the protein incubated with Protein A Sepharose beads (Sigma-Aldrich Co.) using 10 mls of swollen beads/litre of concentrated supernatant at 1 litre of supernatant/5 litre conical flask or beaker. The pH was adjusted to pH 8.5 with 2.75 M Tris, pH 8.5 to allow binding of the CD28TFc to Protein A overnight with gentle stirring at 4° C.
The beads were allowed to settle for 2 h prior to siphoning off the depleted supernatant. The settled beads were then transferred to 50 ml Falcon tubes (Becton Dickinson Biosciences, UK) and recovered by centrifugation at 200 g. The beads were transferred to a 30 ml column (Biorad, UK) and then washed with 300 mls of cold phosphate-buffered saline, 0.5 M NaCl, pH 8.3. The column was eluted with 0.1 M citric acid, pH 3.0 into 10×2 ml fractions in glass tubes containing 0.4 ml of 2.75M Tris, pH 8.5 for immediate neutralization of the citric acid. Fractions containing CD28TFc according to analysis by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) were concentrated to 0.5 ml or no higher concentration than 20 mg/ml using a Centriprep 10 concentrator (Millipore Corp). The protein was then applied to a Superdex 200 H/R gel filtration column (Amersham Biosciences) pre-equilibrated with 10 mM Hepes, 140 mM NaCl, pH 7.4 (HBS buffer) for up to three successive runs. Eluting fractions were monitored for absorbance at 280 nm. Protein-containing fractions were examined by SDS-PAGE. Each cycle of batch purification yielded  ̃9 mgs of CD28TFc; up to 7 batch purifications was required to deplete all the CD28TFc per set of 6 cell factories ( ̃5 liters of starting tissue culture supernatant).
(iv) Thrombin Protease Cleavage of CD28TFc
The most-pure fractions containing CD28TFc were pooled for thrombin cleavage. Initial trial titrations dictated the use of the following conditions for large-scale cleavage of CD2TFc: lyophilized thrombin (Sigma-Aldrich Co.) was re-hydrated in HBS buffer to a concentration of 1 U/μl and this was then added to the CD28TFc at 0.11 μl/6 μg of protein for overnight digestion at 37° C. The reaction was stopped by addition of freshly re-hydrated benzamidine to a final concentration of 1 mM. A 12% non-reducing SDS-PAGE gel was used to confirm the extent of cleavage.
(v) Purification of the CD28 Homodimer
The pH of the thrombin-cleaved protein was adjusted to pH 8.5 using 2.75 M Tris pH 8.5, prior to concentration to 0.5 ml using a Centriprep 10 concentrator (Millipore Corp). Fresh Protein A beads were washed and rehydrated to a final volume of  ̃5 mls, prior to being packed into a 0.7 cm×20 cm Econo-column (Bio-Rad, U.K.) and then equilibriated with HBS, pH 8.5 at 4° C. The concentrated protein was then applied to the column, allowed to run into the bed, and then sequential fractions were eluted by addition of 0.5 ml of HBS, pH 8.5 to the top of the bed every 10 minutes for 2 h. The absorbance of each fraction was determined at 280 nm. The extent of separation of the Fc from the thrombin-released CD28 homodimer was determined by 12% SDS-PAGE analysis of the fractions under non-reducing conditions. The critical steps for good separation were (1) to allow the protein to pass slowly through the column and (2) to conduct the separation at 4° C. The homodimer was concentrated to 0.5 ml and subjected to gel-filtration on a Superdex 75 H/R column (Amersham Biosciences). The purified homodimer was used for crystallization trials, reduced and alkylated for other crystallization trials (see below), or frozen at −80° C. for future use.
Preparation of Fab Fragments of 5.11A1 Antibody
Fab fragments were prepared using the Pierce Biotechnology ImmunoPure® Fab Preparation Kit, as briefly outlined below.
(i) Fab Fragment Generation and Purification
Nine millilitres of whole, purified 5.11A1 antibody at 0.3 mg/ml in PBS was concentrated to 1 ml and then diluted to 10 mls with 20 mM sodium phosphate, 10 mM EDTA, pH 7 and then re-concentrated to 0.5 ml. To this was added 0.5 ml of 20 mM sodium phosphate, pH 7 containing 3.5 mg/ml cysteine●HCl. The 1 ml mixture was then added to 0.5 ml of a 50% slurry of Sepharose-immobilized Papain supplied with the kit, which had been pre-equilibrated with 20 mM sodium phosphate pH 7 containing 3.5 mg/ml cysteine-HCl. This was then incubated for 5 hours in a shaking water bath at 37° C. The cleaved Fab and Fc fragments and undigested IgG were separated from the Immobilized Papain beads by centrifugation at 1000 g and the beads rinsed with 1.5 ml of the ImmunoPure IgG Binding Buffer supplied with the kit. The wash was then combined with the crude digest and the mix applied to a Sepharose-immobilized Protein A column pre-equilibrated with 13 ml of ImmunoPure IgG Binding Buffer. The column was washed with 6.0 ml of the Binding Buffer and the eluate containing the Fab fragments collected (9 mls in total). The eluate was concentrated to 0.5 ml and applied to a Superdex 200 H/R gel filtration column (Amersham Biosciences) pre-equilibrated with HBS buffer. Eluting fractions were monitored for absorbance at 280 nm. Protein-containing fractions were examined by SDS-PAGE.
Preparation of a Crystallizable Form of CD28
(i) Reduction and Alkylation of CD28
CD28 has an interchain disulphide bond within the “stalk-like” region that is largely responsible for homodimerization. It was speculated that its location within the stalk would render it more sensitive to reducing agents than the “canonical” disulphide bond and one other disulphide buried within the ligand-binding domain. If true this meant that monomeric CD28 could be generated which was native-like and fully active for ligand and antibody binding. The minimum concentration of reducing agent required to release the monomer was ascertained by titrating the reducing agent, dithiothreitol (DTF). The reduced cysteines in the protein and excess DTT were each then inactivated by alkylation with a 2.2-fold molar excess of iodoacetamide (IAA). This titration indicated that the optimal conditions were 12.5 μM protein, 1.5 mM DTT and 3.3 mM IAA. The reduced protein sample, generally  ̃4 ml, was finally concentrated to 0.5 ml and separated from unreduced homodimer by gel-filtration on a Superdex 75 H/R column (Amersham Biosciences).
Crystallographic Methods
(i) Crystallization and Data Collection
The CD28/5.11A1 Fab complex was formed by incubating a 2:1 molar ratio of CD28 and Fab, followed by concentration of the protein mixture to 15.6 mg/ml in HBS (the extinction coefficient, ε, of CD28 is 1.7 and that of the Fab is 1.4). Crystals were grown at 18° C. in 0.2 μl hanging drops (0.1 μl of the protein mixture plus 0.1 μl of precipitating reagent) set up using a Cartesian Robot (APS Robotics & Integration, llc; Brown et al (2003) J. Appl. Cryst. 36, 315-18). The precipitating reagent consisted of a mixture of 0.2 M magnesium formate and 20% polyethylene glycol 3350 in water. Crystals appeared in 5-7 days and were cooled to 100K in the precipitating reagent with glycerol added to a final concentration of 10%, using a Cryojet liquid nitrogen system for data collection (Oxford Instruments, Abingdon, UK). Crystals belong to space group C2 with unit cell dimensions a=191.2, b=47.4, c=71.8 Å and a calculated solvent content of 56-58% for one CD28 monomer and one Fab molecule per asymmetric unit (ignoring the effects of glycosylation). The 2.7 Å resolution data were collected from a single crystal at 100K at Beamline ID2 at the European Synchrotron Radiation Facility using a FReLoN CCD. One hundred and fifty-eight 1° rotation images were collected and reduced with HKL2000 (Otwinowski and Minor (1997) Methods Enzym. 276, 307-26).
(ii) Structure Determination and Refinement
The structure was solved by molecular replacement in XPLOR v3.851 (Brünger (1992) X-PLOR Version 3.1. A system for X-ray crystallography and NMR. Yale University Press, New Haven, Conn.) using a set of search models generated from a single Fab structure (PDB accession no. 15c8) by varying the elbow angle in 2° steps using an XPLOR script. A single unambiguous solution was found when the elbow angle was altered by −8° as defined by the script. After rigid body refinement of each domain of the Fab in CNS (Brünger et al. (1998) Acta Cryst. D54, 905-21), the R-factor was 44.4%. Initial electron density maps phased with Fab alone showed very limited, if any, electron density for the CD28 molecule. The Fab model was mutated to the sequence of 5.11A1 before proceeding with two cycles of manual rebuilding in O (Jones et al (1990) O: A macromolecular modelling environment. Crystallographic and modeling methods in molecular design. Bugg and Ealick, Eds. Springer-Verlag Press 189-95) and positional refinement in CNS, reducing the R-factor to 36.4%. A molecular replacement solution for CD28 was then found by using only regions of human CTLA-4 (from PDB 1i81) with strong sequence homology to CD28, i.e. all the β-strands (except C′) and the EF and FG loops, as a search model and by fixing the refined Fab structure. In order to avoid model bias, the refined constant regions of the Fab were replaced with the unaltered constant domains from PDB 15c8 by superposition using SHP (Stuart et al (1979) J. Mol. Biol. 134, 109-42), and the positions were fixed during refinement. The variable regions of the Fab and the CD28 monomer were refined in X-PLOR v3.851 using positional refinement followed by grouped and restrained individual B factor refinement along with manual rebuilding in O. All refinement procedures used data from 25.0-2.7 Å and excluded 5% of reflections randomly selected for generation of the Rfree data set. The anisotropy of the data was corrected for using X-PLOR and the data were sharpened for all but the last round of refinement. The current model has an R factor of 25.3% and an Rfree of 33.4% against all data to 2.7 Å. The model includes the CD28 monomer (excluding its C-terminal stalk) from residues 2 to 118, excluding residues Leu28 and Phe29, which are poorly ordered, and the Fab, but with none of the sugar chains built onto the CD28. The model has 76% of residues in the most favoured regions of the Ramachandran plot, with a further 21% in additionally allowed regions.
The Structure of a CD28-Superagonist Antibody Complex
The ligand-binding V-set IgSF domain of the CD28 monomer, solved in complex with 5.11A1 Fab, is very similar to the equivalent region of CTLA-4. Lattice contacts generate a plausible CD28 homodimer. Whilst this dimer is similar to the CTLA-4 homodimer (which is bivalent), the arrangement of the monomers in CD28 is sufficiently distinct to suggest an explanation for the apparent monovalence of CD28, i.e. the membrane proximal domains of ligands competing for each ligand binding site are likely to clash. Cryoelectron microscopic analyses show that both classes of antibodies are bivalent, ruling out aggregation-based explanations for the differential signalling effects of conventional versus superagonistic antibodies. The 5.11A1 Fab fragment binds orthogonally to the surface formed by the CC′C′′ strands of CD28, favouring the C′C′′ “edge” of the monomer. This places the two long axes of the Fabs parallel to each other and the cell surface. In contrast, the epitopes of the conventional antibodies are close to the ligand binding “top” of the homodimer.
Signalling by Receptors Dependent on Extrinsic Kinases
The existence of two classes of activating anti-CD28 antibodies, i.e. costimulatory antibodies and superagonists, greatly simplifies the analysis of signalling by this archetypal costimulatory receptor. The observation that the two antibody classes have homologous effects in two different species, and that these effects are predictable simply according to the location of their epitopes, rules out trivial explanations for the distinct signalling properties of these antibodies, e.g. affinity differences. Instead, the receptor-triggering problem is reduced to a comparison of the structural properties of the complexes that these two types of antibodies form with CD28.
From the present crystal structure of the complex of CD28 with a superagonistic antibody, it can be deduced that an antibody-induced increase in the net phosphorylation of the cytoplasmic domain of a given cell surface receptor, due to changes in the rates of phosphorylation and dephosphorylation of it, results in the triggering of that receptor (see FIG. 1). In more detail, the antibodies hold the cell surface at certain distances from an immobilizing substrate (i.e. other, Fc receptor-bearing cells, in vivo or plastic, in vitro), such that the membrane separation in the region of the immobilized antibody and receptor will differentially exclude, from the immediate vicinity of the receptor, other molecules whose extracellular domains are comparable in size or larger than CD28-antibody complexes, such as the tyrosine phosphatase, CD45. In contrast, tyrosine kinases, e.g. p56lck, will be unaffected because they are small and/or attached to the inner leaflet of the membrane. The result is that, overall, the phosphorylation of CD28 will be favoured over its de-phoshorylation (by CD45), with the net increase in phosphorylation amounting to receptor triggering. Superagonists are more potent than conventional antibodies because they bind epitopes close to the membrane rather than at the “top” of the molecule, leading to more efficient segregation of, e.g. CD45, and therefore a larger increase in the net phosphorylation of CD28.
The important corollary of this explanation is that antibodies that bind the membrane-proximal epitopes of the large number of other cell surface molecules not belonging to the CD28 subset of the immunoglobulin superfamily, but sharing the key signalling property of CD28-subset molecules, i.e. a reliance on extrinsic tyrosine kinases, for example PD-1 and BTLA, should also potently invoke the activating or inhibitory signalling properties of those molecules in precisely the same way, allowing the manipulation of cell behaviour with such antibodies.
PD-1 and BTLA
Programmed Death (PD)-1 protein modulates the responses of previously activated T- and B-cells in secondary lymphoid organs or peripheral (parenchymal) tissues. PD-1 is expressed on a subset of thymocytes and upregulated on activated T, B and myeloid cells. The PD-1 ligands, B7-H1 and B7-DC, are more widely expressed than those of CD28 family members and are induced by pro-inflammatory agents on monocytes and dendritic cells and on activated but not resting B cells. B7-DC is expressed in a variety of peripheral tissues, including heart, pancreas, lung and liver. The B and T lymphocyte attenuator (BTLA), is also expressed on activated T and B cells, but attenuates production of IL-2 after binding a different peripherally expressed B7-related protein, called B7x.
PD-1 and BTLA are structurally comparable to other members of the CD28 family only insofar as the proteins also consist of a single V-set IgSF domain supported on stalks, and the cytoplasmic domains contain immunoreceptor tyrosine-based motifs (inhibitory in these two cases). The sequences of the IgSF domains of PD-1 and BTLA indicate that these proteins are unrelated and do not belong to the same subset of the IgSF as CD28. Consistent with the view that PD-1 is involved in maintaining tolerance in normal T cells in the periphery, mice lacking the gene develop a variety of autoimmune-like diseases dependent on the genetic background, including lupus-like arthritis and glomerulonephritis, and have increased serum IgG3 and augmented B-cell proliferative responses to anti-IgM in vivo.
Activating signalling by PD-1 and BTLA, because these molecules are inhibitory, would be expected to dampen a variety of peripheral immune pathologies. Signalling of this type could be stimulated by the binding of superagonistic antibodies to the membrane proximal regions of these proteins. A set of additional molecules, each of which has inhibitory immunoreceptor tyrosine-based signalling motifs and are therefore targets for generating superagonists, is shown in Table 2.
Identifying Epitopes for Superagonists
The procedure is as follows. The “lower”, membrane-proximal half of the membrane proximal domain of a given structure, e.g. CD28 in FIG. 2A, is identified by visual inspection of the structure. Superagonistic agents bind residues in this region. For identifying superagonistic agents in receptors closely related to the known structure, the equivalent regions are identified in an alignment of the sequence of the known structure with that of the related proteins as shown in FIG. 2A. To identify the epitopes of superagonistic antibodies in other molecules that have the requisite signalling properties but for which there is no structure, e.g. PD-1 in FIG. 2B, a structural model is identified by alignment of the sequence with that of the most similar molecule of known structure in the Protein Data Bank (in the example in FIG. 2B, the variable domain of the Ig kappa chain is the structure with the most similar sequence to PD-1). The “lower”, membrane proximal half of this domain is highlighted on the structure by visual inspection and the equivalent regions are identified in the sequence alignment. To screen for superagonists, the second and third residues “down” (i.e. toward the membrane) in the marked epitopes shown in parts A and B are identified as shown in FIGS. 2C and 2D. These residues, singly or together preferably form at least part of the epitopes of antibodies or other superagonistic agents, as binding to these residues will require that the binding of the agent is parallel with the cell membrane. These residues can therefore be mutated in proteins used for screening the superagonistic agents (i.e. agents binding to the unmutated protein but not the mutated protein, should be superagonistic).








TABLE 1







CD28 family superagonistic epitopes



Epitopes are named according to the strands from


which they derive.














Protein
A′
B
C-C′
C′′-D
E
F
G


















hCD28
SPMLV
AVNLS
SLHKGLDSAVEVCV
VYSKTGFNCDG
FYLQN
TDIYFC
NGTIIHV



hCTLA-4
PAVVL
GIASFV
TVLRQADSQVTEVCA
FLDDSICTG
LTIQG
TGLYIC
NGTQIYV


hICOS
YEMFI
GVQIL
QLLKGGQILCD
VSIKSLKFCHS
FFLYN
ANYYFC
TGGYLHI



(SEQ ID
(SEQ ID
(SEQ ID NOs:
(SEQ ID NOs: 32,
(SEQ ID
(SEQ ID
(SEQ ID



NOs: 23,
NOs: 26,
29, 30, 31)
33, 34)
NOs: 35, 36,
NOs: 38, 39,
NOs: 41, 42,



24, 25)
27, 28)


37)
40)
43)










PD-1 and BTLA superagonistic epitopes














Protein
A
B
C-C′
C′′-D
E
F
G


















hpd-1
PALLVV
DNATF
RMSPSNQTDK
QPGQDCRFR
MSVVR
NDSGTY
LRAELR



hBTLA
QSEHSI
DPFEL
KLNG
QTSWK
LHFEP
NDNGSY
TTLYVT



(SEQ ID
(SEQ ID
(SEQ ID NOs: 48,
(SEQ ID NOs:
(SEQ ID
(SEQ ID
(SEQ ID



NOs: 44, 45)
NOs: 46, 47)
49)
50, 51)
NOs: 52, 53)
NOs: 54, 55)
NOs: 56, 57)



















TABLE 3







Protein
Sequence




















hCD28
GNYSQQLQVYSKTGF





(SEQ ID NO: 58)



 



hCTLA-4
YMMGNELTFLDDS




(SEQ ID NO: 59)



 



hICOS
KTKGSGNTVSIKSLK




(SEQ ID NO: 60)



 



hPD-1
LAAFPEDRSQPGQDCR




(SEQ ID NO: 61)

















TABLE 2







Targets of inhibitory superagonist antibodies










Receptor
Chromosome
Expression
no. of ITIMs





FcRIIB
1q23-24
B, myeloid, mast
1


PILR
7q22
Myeloid
2


CD72
9p
B
2


CD5
11q13
T, subset B
1


MAFA
12p12-13
Myeloid, mast, NK
1


NKG2A
12p13.1-13.2
NK, T
2


CD31
17q23
Myeloid, etc
1


CMRF35H
17q24
Leukocytes
3


CD22
19q13.1
B
4


CD66a
19q13.2
Granulocytes, etc
2


CD66d
19q13.2
Granulocytes
1


CD33
19q13.3
Myeloid
1


SIGLEC5
19q13.3
Myeloid
1


SIGLEC6
19q13.3
B cells, myeloid
1


SIGLEC7
19q13.3
NK, myeloid, etc
1


ILT2, 3, 4, 5
19q13.4
Myeloid, B, etc
4


LIR8
19q13.4
Myeloid, B, etc
4


LAIR-1
19q13.4
Leukocytes
2


KIR2DL
19q13.4
NK, T
2


KIR3DL
19q13.4
NK, T
2


SIRP
20p13
Myeloid, etc
2
















TABLE 4





Co-ordinates of the CD28/5.11A1 crystal structure















REMARK FILENAME = “/mnt/safe6/ed/cd28/cd28_all/xplor/ref18/positionaln3.pdb”


REMARK R = 0.251035 from 25 to 2.7


REMARK DATE: Jan. 09, 2004 09:48:19 created by user: robert

















ATOM
1
CB
ASP
1
122.280
49.781
38.327
1.00
64.18
L


ATOM
2
CG
ASP
1
123.319
49.071
37.463
1.00
67.78
L


ATOM
3
OD1
ASP
1
122.934
48.417
36.470
1.00
66.82
L


ATOM
4
OD2
ASP
1
124.524
49.162
37.779
1.00
54.04
L


ATOM
5
C
ASP
1
122.104
52.265
38.005
1.00
50.51
L


ATOM
6
O
ASP
1
122.541
53.057
37.163
1.00
41.52
L


ATOM
7
N
ASP
1
121.674
50.711
36.112
1.00
37.53
L


ATOM
8
CA
ASP
1
121.547
50.905
37.579
1.00
50.29
L


ATOM
9
N
ILE
2
122.073
52.527
39.314
1.00
51.24
L


ATOM
10
CA
ILE
2
122.567
53.783
39.890
1.00
50.84
L


ATOM
11
CB
ILE
2
121.842
54.094
41.240
1.00
22.62
L


ATOM
12
CG2
ILE
2
122.278
55.454
41.781
1.00
18.55
L


ATOM
13
CG1
ILE
2
120.327
54.081
41.022
1.00
30.61
L


ATOM
14
CD1
ILE
2
119.510
54.521
42.229
1.00
31.86
L


ATOM
15
C
ILE
2
124.086
53.714
40.119
1.00
54.91
L


ATOM
16
O
ILE
2
124.645
52.630
40.283
1.00
55.68
L


ATOM
17
N
GLN
3
124.749
54.869
40.136
1.00
58.93
L


ATOM
18
CA
GLN
3
126.204
54.923
40.322
1.00
56.95
L


ATOM
19
CB
GLN
3
126.847
55.679
39.145
1.00
60.02
L


ATOM
20
CG
GLN
3
127.010
54.851
37.863
1.00
63.51
L


ATOM
21
CD
GLN
3
126.479
55.552
36.615
1.00
65.36
L


ATOM
22
OE1
GLN
3
127.024
56.568
36.174
1.00
62.07
L


ATOM
23
NE2
GLN
3
125.412
55.003
36.038
1.00
72.37
L


ATOM
24
C
GLN
3
126.635
55.564
41.650
1.00
51.69
L


ATOM
25
O
GLN
3
126.179
56.649
42.004
1.00
54.63
L


ATOM
26
N
MET
4
127.522
54.889
42.376
1.00
42.42
L


ATOM
27
CA
MET
4
128.005
55.392
43.660
1.00
37.64
L


ATOM
28
CB
MET
4
128.066
54.248
44.671
1.00
54.55
L


ATOM
29
CG
MET
4
127.037
54.319
45.783
1.00
56.74
L


ATOM
30
SD
MET
4
125.360
54.633
45.189
1.00
76.06
L


ATOM
31
CE
MET
4
125.083
56.269
45.843
1.00
68.01
L


ATOM
32
C
MET
4
129.392
56.008
43.507
1.00
44.16
L


ATOM
33
O
MET
4
130.358
55.311
43.176
1.00
35.72
L


ATOM
34
N
ASN
5
129.496
57.310
43.750
1.00
50.40
L


ATOM
35
CA
ASN
5
130.779
57.996
43.624
1.00
58.90
L


ATOM
36
CB
ASN
5
130.703
59.060
42.521
1.00
67.94
L


ATOM
37
CG
ASN
5
130.113
58.517
41.233
1.00
59.91
L


ATOM
38
OD1
ASN
5
130.783
57.811
40.474
1.00
49.08
L


ATOM
39
ND2
ASN
5
128.848
58.839
40.981
1.00
56.43
L


ATOM
40
C
ASN
5
131.191
58.643
44.939
1.00
46.55
L


ATOM
41
O
ASN
5
130.612
59.637
45.368
1.00
40.94
L


ATOM
42
N
GLN
6
132.202
58.076
45.577
1.00
33.77
L


ATOM
43
CA
GLN
6
132.661
58.607
46.846
1.00
36.85
L


ATOM
44
CB
GLN
6
133.257
57.476
47.679
1.00
24.74
L


ATOM
45
CG
GLN
6
132.395
56.218
47.684
1.00
19.84
L


ATOM
46
CD
GLN
6
132.393
55.524
49.029
1.00
27.56
L


ATOM
47
OE1
GLN
6
132.925
56.048
50.013
1.00
30.84
L


ATOM
48
NE2
GLN
6
131.803
54.339
49.082
1.00
34.41
L


ATOM
49
C
GLN
6
133.677
59.725
46.647
1.00
43.10
L


ATOM
50
O
GLN
6
134.328
59.800
45.608
1.00
55.89
L


ATOM
51
N
SER
7
133.807
60.598
47.641
1.00
47.45
L


ATOM
52
CA
SER
7
134.736
61.714
47.543
1.00
54.98
L


ATOM
53
CB
SER
7
134.285
62.858
48.469
1.00
70.71
L


ATOM
54
OG
SER
7
135.232
63.917
48.514
1.00
61.37
L


ATOM
55
C
SER
7
136.187
61.304
47.812
1.00
57.75
L


ATOM
56
O
SER
7
136.828
60.732
46.928
1.00
68.97
L


ATOM
57
N
PRO
8
136.722
61.540
49.029
1.00
48.65
L


ATOM
58
CD
PRO
8
136.250
62.116
50.300
1.00
21.82
L


ATOM
59
CA
PRO
8
138.118
61.109
49.140
1.00
47.63
L


ATOM
60
CB
PRO
8
138.515
61.533
50.559
1.00
29.91
L


ATOM
61
CG
PRO
8
137.525
62.576
50.933
1.00
26.41
L


ATOM
62
C
PRO
8
138.358
59.622
48.893
1.00
48.77
L


ATOM
63
O
PRO
8
137.820
58.772
49.604
1.00
63.86
L


ATOM
64
N
SER
9
139.161
59.318
47.876
1.00
33.02
L


ATOM
65
CA
SER
9
139.500
57.939
47.548
1.00
32.48
L


ATOM
66
CB
SER
9
140.151
57.864
46.169
1.00
30.85
L


ATOM
67
OG
SER
9
141.554
57.812
46.286
1.00
25.75
L


ATOM
68
C
SER
9
140.469
57.407
48.602
1.00
30.90
L


ATOM
69
O
SER
9
140.661
56.197
48.737
1.00
32.96
L


ATOM
70
N
SER
10
141.076
58.333
49.340
1.00
29.68
L


ATOM
71
CA
SER
10
142.022
58.018
50.412
1.00
35.34
L


ATOM
72
CB
SER
10
143.387
57.645
49.831
1.00
49.82
L


ATOM
73
OG
SER
10
144.392
58.538
50.289
1.00
81.38
L


ATOM
74
C
SER
10
142.161
59.254
51.308
1.00
31.04
L


ATOM
75
O
SER
10
141.964
60.379
50.839
1.00
35.88
L


ATOM
76
N
LEU
11
142.502
59.056
52.582
1.00
27.16
L


ATOM
77
CA
LEU
11
142.634
60.181
53.498
1.00
24.50
L


ATOM
78
CB
LEU
11
141.258
60.780
53.780
1.00
47.56
L


ATOM
79
CG
LEU
11
141.120
61.488
55.134
1.00
57.17
L


ATOM
80
CD1
LEU
11
141.752
62.878
55.063
1.00
65.58
L


ATOM
81
CD2
LEU
11
139.650
61.583
55.509
1.00
61.17
L


ATOM
82
C
LEU
11
143.308
59.884
54.831
1.00
22.63
L


ATOM
83
O
LEU
11
142.978
58.919
55.508
1.00
25.60
L


ATOM
84
N
SER
12
144.233
60.759
55.209
1.00
33.11
L


ATOM
85
CA
SER
12
144.965
60.653
56.466
1.00
35.62
L


ATOM
86
CB
SER
12
146.465
60.745
56.201
1.00
34.92
L


ATOM
87
OG
SER
12
147.186
60.895
57.409
1.00
29.53
L


ATOM
88
C
SER
12
144.534
61.798
57.392
1.00
38.38
L


ATOM
89
O
SER
12
144.226
62.894
56.928
1.00
46.48
L


ATOM
90
N
ALA
13
144.519
61.546
58.696
1.00
31.39
L


ATOM
91
CA
ALA
13
144.116
62.559
59.666
1.00
34.62
L


ATOM
92
CB
ALA
13
142.615
62.610
59.753
1.00
28.98
L


ATOM
93
C
ALA
13
144.706
62.178
61.012
1.00
38.12
L


ATOM
94
O
ALA
13
144.928
61.002
61.268
1.00
41.17
L


ATOM
95
N
SER
14
144.946
63.156
61.878
1.00
36.11
L


ATOM
96
CA
SER
14
145.553
62.865
63.177
1.00
42.50
L


ATOM
97
CB
SER
14
146.477
64.012
63.593
1.00
45.35
L


ATOM
98
OG
SER
14
147.608
63.522
64.299
1.00
51.37
L


ATOM
99
C
SER
14
144.618
62.553
64.343
1.00
36.84
L


ATOM
100
O
SER
14
143.395
62.586
64.217
1.00
30.77
L


ATOM
101
N
LEU
15
145.246
62.246
65.477
1.00
42.69
L


ATOM
102
CA
LEU
15
144.588
61.919
66.737
1.00
51.12
L


ATOM
103
CB
LEU
15
145.077
62.874
67.829
1.00
35.76
L


ATOM
104
CG
LEU
15
145.549
64.244
67.331
1.00
50.27
L


ATOM
105
CD1
LEU
15
144.709
65.353
67.956
1.00
45.12
L


ATOM
106
CD2
LEU
15
147.023
64.425
67.671
1.00
46.79
L


ATOM
107
C
LEU
15
143.071
61.968
66.666
1.00
63.00
L


ATOM
108
O
LEU
15
142.440
61.062
66.125
1.00
71.25
L


ATOM
109
N
GLY
16
142.495
63.029
67.229
1.00
61.62
L


ATOM
110
CA
GLY
16
141.052
63.182
67.225
1.00
58.47
L


ATOM
111
C
GLY
16
140.588
64.176
66.177
1.00
62.05
L


ATOM
112
O
GLY
16
139.827
65.094
66.481
1.00
59.73
L


ATOM
113
N
ASP
17
141.052
63.991
64.942
1.00
62.73
L


ATOM
114
CA
ASP
17
140.690
64.863
63.828
1.00
55.42
L


ATOM
115
CB
ASP
17
141.729
64.728
62.715
1.00
45.39
L


ATOM
116
CG
ASP
17
142.006
66.039
62.017
1.00
56.02
L


ATOM
117
OD1
ASP
17
142.467
66.992
62.682
1.00
51.84
L


ATOM
118
OD2
ASP
17
141.762
66.116
60.795
1.00
64.45
L


ATOM
119
C
ASP
17
139.313
64.470
63.298
1.00
54.85
L


ATOM
120
O
ASP
17
139.066
63.296
63.027
1.00
69.38
L


ATOM
121
N
THR
18
138.425
65.448
63.150
1.00
39.55
L


ATOM
122
CA
THR
18
137.073
65.189
62.661
1.00
35.18
L


ATOM
123
CB
THR
18
136.089
66.238
63.211
1.00
39.72
L


ATOM
124
OG1
THR
18
136.155
66.250
64.644
1.00
36.21
L


ATOM
125
CG2
THR
18
134.668
65.918
62.774
1.00
28.85
L


ATOM
126
C
THR
18
136.967
65.172
61.132
1.00
39.85
L


ATOM
127
O
THR
18
136.590
66.167
60.522
1.00
51.97
L


ATOM
128
N
ILE
19
137.289
64.035
60.521
1.00
38.56
L


ATOM
129
CA
ILE
19
137.233
63.881
59.065
1.00
40.49
L


ATOM
130
CB
ILE
19
137.917
62.579
58.621
1.00
43.22
L


ATOM
131
CG2
ILE
19
139.334
62.545
59.144
1.00
53.54
L


ATOM
132
CG1
ILE
19
137.148
61.373
59.161
1.00
31.99
L


ATOM
133
CD1
ILE
19
136.020
60.889
58.260
1.00
27.22
L


ATOM
134
C
ILE
19
135.811
63.857
58.519
1.00
41.63
L


ATOM
135
O
ILE
19
134.850
63.825
59.284
1.00
48.28
L


ATOM
136
N
THR
20
135.690
63.839
57.191
1.00
41.74
L


ATOM
137
CA
THR
20
134.388
63.814
56.517
1.00
46.28
L


ATOM
138
CB
THR
20
133.761
65.246
56.473
1.00
55.85
L


ATOM
139
OG1
THR
20
132.666
65.316
57.396
1.00
68.15
L


ATOM
140
CG2
THR
20
133.260
65.592
55.073
1.00
57.90
L


ATOM
141
C
THR
20
134.516
63.260
55.090
1.00
34.62
L


ATOM
142
O
THR
20
135.433
63.620
54.351
1.00
35.05
L


ATOM
143
N
ILE
21
133.592
62.385
54.706
1.00
27.66
L


ATOM
144
CA
ILE
21
133.613
61.793
53.371
1.00
40.68
L


ATOM
145
CB
ILE
21
133.646
60.260
53.466
1.00
45.17
L


ATOM
146
CG2
ILE
21
133.946
59.641
52.110
1.00
58.28
L


ATOM
147
CG1
ILE
21
134.757
59.849
54.432
1.00
37.17
L


ATOM
148
CD1
ILE
21
134.556
58.489
55.038
1.00
30.25
L


ATOM
149
C
ILE
21
132.412
62.266
52.552
1.00
41.14
L


ATOM
150
O
ILE
21
131.706
63.173
52.978
1.00
52.31
L


ATOM
151
N
THR
22
132.176
61.669
51.386
1.00
37.16
L


ATOM
152
CA
THR
22
131.069
62.096
50.533
1.00
30.97
L


ATOM
153
CB
THR
22
131.426
63.392
49.791
1.00
33.17
L


ATOM
154
OG1
THR
22
131.754
64.417
50.736
1.00
20.87
L


ATOM
155
CG2
THR
22
130.270
63.832
48.913
1.00
26.45
L


ATOM
156
C
THR
22
130.703
61.078
49.462
1.00
45.82
L


ATOM
157
O
THR
22
131.563
60.656
48.694
1.00
54.24
L


ATOM
158
N
CYS
23
129.430
60.705
49.381
1.00
50.07
L


ATOM
159
CA
CYS
23
129.004
59.746
48.363
1.00
63.67
L


ATOM
160
C
CYS
23
127.790
60.230
47.570
1.00
68.93
L


ATOM
161
O
CYS
23
126.651
60.017
47.983
1.00
85.32
L


ATOM
162
CB
CYS
23
128.682
58.384
48.998
1.00
53.28
L


ATOM
163
SG
CYS
23
127.931
57.193
47.829
1.00
60.78
L


ATOM
164
N
HIS
24
128.035
60.874
46.432
1.00
62.30
L


ATOM
165
CA
HIS
24
126.958
61.376
45.581
1.00
55.79
L


ATOM
166
CB
HIS
24
127.438
62.585
44.766
1.00
66.11
L


ATOM
167
CG
HIS
24
127.210
63.903
45.443
1.00
94.47
L


ATOM
168
CD2
HIS
24
127.360
64.280
46.736
1.00
100.00
L


ATOM
169
ND1
HIS
24
126.760
65.020
44.771
1.00
99.99
L


ATOM
170
CE1
HIS
24
126.647
66.029
45.618
1.00
96.64
L


ATOM
171
NE2
HIS
24
127.005
65.605
46.818
1.00
100.00
L


ATOM
172
C
HIS
24
126.456
60.282
44.636
1.00
53.07
L


ATOM
173
O
HIS
24
127.232
59.442
44.174
1.00
50.30
L


ATOM
174
N
ALA
25
125.154
60.295
44.359
1.00
48.84
L


ATOM
175
CA
ALA
25
124.546
59.309
43.470
1.00
62.65
L


ATOM
176
CB
ALA
25
123.334
58.678
44.149
1.00
50.68
L


ATOM
177
C
ALA
25
124.135
59.923
42.124
1.00
71.88
L


ATOM
178
O
ALA
25
123.769
61.098
42.053
1.00
69.35
L


ATOM
179
N
SER
26
124.196
59.120
41.061
1.00
75.26
L


ATOM
180
CA
SER
26
123.833
59.585
39.724
1.00
65.26
L


ATOM
181
CB
SER
26
123.832
58.421
38.724
1.00
51.17
L


ATOM
182
OG
SER
26
122.779
57.511
38.981
1.00
34.32
L


ATOM
183
C
SER
26
122.466
60.247
39.755
1.00
68.59
L


ATOM
184
O
SER
26
122.329
61.422
39.413
1.00
70.72
L


ATOM
185
N
GLN
27
121.452
59.490
40.163
1.00
70.13
L


ATOM
186
CA
GLN
27
120.103
60.030
40.253
1.00
61.69
L


ATOM
187
CB
GLN
27
119.171
59.312
39.273
1.00
49.30
L


ATOM
188
CG
GLN
27
119.385
57.827
39.181
1.00
40.46
L


ATOM
189
CD
GLN
27
118.295
57.064
39.899
1.00
47.76
L


ATOM
190
OE1
GLN
27
117.623
57.601
40.785
1.00
42.99
L


ATOM
191
NE2
GLN
27
118.110
55.806
39.523
1.00
37.30
L


ATOM
192
C
GLN
27
119.568
59.937
41.690
1.00
59.24
L


ATOM
193
O
GLN
27
120.323
59.658
42.623
1.00
58.99
L


ATOM
194
N
ASN
28
118.268
60.160
41.859
1.00
54.50
L


ATOM
195
CA
ASN
28
117.628
60.162
43.176
1.00
48.17
L


ATOM
196
CB
ASN
28
116.286
60.871
43.058
1.00
28.87
L


ATOM
197
CG
ASN
28
115.671
61.159
44.397
1.00
25.62
L


ATOM
198
OD1
ASN
28
116.346
61.607
45.321
1.00
43.90
L


ATOM
199
ND2
ASN
28
114.383
60.894
44.518
1.00
47.96
L


ATOM
200
C
ASN
28
117.422
58.833
43.917
1.00
53.06
L


ATOM
201
O
ASN
28
116.727
57.939
43.431
1.00
50.76
L


ATOM
202
N
ILE
29
118.011
58.727
45.112
1.00
49.78
L


ATOM
203
CA
ILE
29
117.887
57.528
45.951
1.00
48.76
L


ATOM
204
CB
ILE
29
119.278
56.900
46.275
1.00
38.91
L


ATOM
205
CG2
ILE
29
119.870
56.276
45.028
1.00
38.09
L


ATOM
206
CG1
ILE
29
120.235
57.965
46.801
1.00
35.93
L


ATOM
207
CD1
ILE
29
121.444
57.381
47.498
1.00
24.13
L


ATOM
208
C
ILE
29
117.176
57.883
47.270
1.00
49.01
L


ATOM
209
O
ILE
29
117.043
57.056
48.183
1.00
28.15
L


ATOM
210
N
TYR
30
116.720
59.127
47.358
1.00
51.14
L


ATOM
211
CA
TYR
30
116.018
59.610
48.536
1.00
40.55
L


ATOM
212
CB
TYR
30
114.708
58.834
48.708
1.00
51.35
L


ATOM
213
CG
TYR
30
113.856
58.829
47.452
1.00
50.82
L


ATOM
214
CD1
TYR
30
112.914
59.832
47.218
1.00
57.07
L


ATOM
215
CE1
TYR
30
112.164
59.859
46.044
1.00
48.93
L


ATOM
216
CD2
TYR
30
114.023
57.846
46.477
1.00
54.94
L


ATOM
217
CE2
TYR
30
113.280
57.863
45.302
1.00
54.60
L


ATOM
218
CZ
TYR
30
112.354
58.872
45.089
1.00
54.60
L


ATOM
219
OH
TYR
30
111.641
58.906
43.914
1.00
46.05
L


ATOM
220
C
TYR
30
116.867
59.539
49.800
1.00
36.49
L


ATOM
221
O
TYR
30
117.747
60.377
50.007
1.00
36.08
L


ATOM
222
N
VAL
31
116.607
58.553
50.650
1.00
27.22
L


ATOM
223
CA
VAL
31
117.363
58.421
51.889
1.00
25.88
L


ATOM
224
CB
VAL
31
116.518
58.771
53.103
1.00
24.60
L


ATOM
225
CG1
VAL
31
115.741
60.036
52.827
1.00
39.18
L


ATOM
226
CG2
VAL
31
115.581
57.607
53.441
1.00
11.43
L


ATOM
227
C
VAL
31
117.815
57.001
52.068
1.00
30.74
L


ATOM
228
O
VAL
31
118.333
56.637
53.125
1.00
32.26
L


ATOM
229
N
TRP
32
117.612
56.202
51.028
1.00
34.27
L


ATOM
230
CA
TRP
32
117.978
54.796
51.064
1.00
29.66
L


ATOM
231
CB
TRP
32
117.016
53.999
50.173
1.00
37.57
L


ATOM
232
CG
TRP
32
115.569
54.306
50.497
1.00
45.16
L


ATOM
233
CD2
TRP
32
114.858
53.921
51.677
1.00
28.80
L


ATOM
234
CE2
TRP
32
113.571
54.493
51.597
1.00
36.20
L


ATOM
235
CE3
TRP
32
115.183
53.149
52.798
1.00
25.93
L


ATOM
236
CD1
TRP
32
114.706
55.074
49.765
1.00
47.52
L


ATOM
237
NE1
TRP
32
113.504
55.193
50.418
1.00
36.87
L


ATOM
238
CZ2
TRP
32
112.610
54.319
52.591
1.00
25.25
L


ATOM
239
CZ3
TRP
32
114.229
52.978
53.788
1.00
21.98
L


ATOM
240
CH2
TRP
32
112.954
53.563
53.677
1.00
2.00
L


ATOM
241
C
TRP
32
119.419
54.578
50.645
1.00
17.38
L


ATOM
242
O
TRP
32
119.697
54.267
49.491
1.00
20.83
L


ATOM
243
N
LEU
33
120.332
54.750
51.599
1.00
19.97
L


ATOM
244
CA
LEU
33
121.763
54.570
51.357
1.00
39.54
L


ATOM
245
CB
LEU
33
122.352
55.790
50.642
1.00
44.19
L


ATOM
246
CG
LEU
33
123.876
55.803
50.500
1.00
34.32
L


ATOM
247
CD1
LEU
33
124.252
56.190
49.078
1.00
27.47
L


ATOM
248
CD2
LEU
33
124.479
56.774
51.515
1.00
19.98
L


ATOM
249
C
LEU
33
122.481
54.371
52.685
1.00
39.15
L


ATOM
250
O
LEU
33
122.345
55.182
53.595
1.00
42.09
L


ATOM
251
N
ASN
34
123.262
53.303
52.785
1.00
38.96
L


ATOM
252
CA
ASN
34
123.969
53.008
54.017
1.00
33.81
L


ATOM
253
CB
ASN
34
123.635
51.588
54.470
1.00
29.67
L


ATOM
254
CG
ASN
34
122.323
51.104
53.908
1.00
18.96
L


ATOM
255
OD1
ASN
34
121.252
51.484
54.384
1.00
22.09
L


ATOM
256
ND2
ASN
34
122.396
50.269
52.883
1.00
22.68
L


ATOM
257
C
ASN
34
125.465
53.162
53.889
1.00
27.35
L


ATOM
258
O
ASN
34
125.996
53.215
52.785
1.00
25.60
L


ATOM
259
N
TRP
35
126.125
53.242
55.041
1.00
31.00
L


ATOM
260
CA
TRP
35
127.574
53.380
55.122
1.00
37.04
L


ATOM
261
CB
TRP
35
127.942
54.663
55.871
1.00
44.59
L


ATOM
262
CG
TRP
35
127.725
55.956
55.099
1.00
50.73
L


ATOM
263
CD2
TRP
35
128.624
56.560
54.155
1.00
39.25
L


ATOM
264
CE2
TRP
35
128.042
57.776
53.738
1.00
36.81
L


ATOM
265
CE3
TRP
35
129.868
56.194
53.621
1.00
29.95
L


ATOM
266
CD1
TRP
35
126.660
56.808
55.208
1.00
38.58
L


ATOM
267
NE1
TRP
35
126.843
57.900
54.397
1.00
40.14
L


ATOM
268
CZ2
TRP
35
128.655
58.629
52.812
1.00
36.05
L


ATOM
269
CZ3
TRP
35
130.479
57.045
52.698
1.00
39.51
L


ATOM
270
CH2
TRP
35
129.871
58.247
52.307
1.00
31.94
L


ATOM
271
C
TRP
35
128.118
52.168
55.885
1.00
32.85
L


ATOM
272
O
TRP
35
127.499
51.715
56.849
1.00
24.56
L


ATOM
273
N
TYR
36
129.271
51.654
55.451
1.00
29.03
L


ATOM
274
CA
TYR
36
129.894
50.488
56.080
1.00
24.01
L


ATOM
275
CB
TYR
36
129.760
49.256
55.178
1.00
24.57
L


ATOM
276
CG
TYR
36
128.341
48.844
54.845
1.00
36.78
L


ATOM
277
CD1
TYR
36
127.740
47.745
55.469
1.00
45.66
L


ATOM
278
CE1
TYR
36
126.439
47.350
55.138
1.00
29.26
L


ATOM
279
CD2
TYR
36
127.606
49.536
53.885
1.00
43.28
L


ATOM
280
CE2
TYR
36
126.314
49.153
53.550
1.00
29.25
L


ATOM
281
CZ
TYR
36
125.736
48.064
54.174
1.00
33.13
L


ATOM
282
OH
TYR
36
124.458
47.709
53.810
1.00
29.39
L


ATOM
283
C
TYR
36
131.379
50.683
56.401
1.00
34.79
L


ATOM
284
O
TYR
36
132.060
51.530
55.813
1.00
31.84
L


ATOM
285
N
GLN
37
131.876
49.880
57.337
1.00
38.75
L


ATOM
286
CA
GLN
37
133.278
49.923
57.731
1.00
41.58
L


ATOM
287
CB
GLN
37
133.406
50.342
59.199
1.00
30.71
L


ATOM
288
CG
GLN
37
134.852
50.495
59.704
1.00
37.19
L


ATOM
289
CD
GLN
37
134.989
50.332
61.222
1.00
25.39
L


ATOM
290
OE1
GLN
37
134.904
49.222
61.753
1.00
36.25
L


ATOM
291
NE2
GLN
37
135.209
51.439
61.918
1.00
22.58
L


ATOM
292
C
GLN
37
133.884
48.528
57.528
1.00
39.79
L


ATOM
293
O
GLN
37
133.319
47.526
57.975
1.00
29.52
L


ATOM
294
N
GLN
38
135.022
48.465
56.839
1.00
29.65
L


ATOM
295
CA
GLN
38
135.695
47.195
56.596
1.00
31.00
L


ATOM
296
CB
GLN
38
135.471
46.738
55.155
1.00
37.06
L


ATOM
297
CG
GLN
38
136.385
45.597
54.724
1.00
37.85
L


ATOM
298
CD
GLN
38
135.973
44.992
53.401
1.00
51.91
L


ATOM
299
OE1
GLN
38
135.856
45.693
52.399
1.00
56.78
L


ATOM
300
NE2
GLN
38
135.753
43.683
53.390
1.00
47.92
L


ATOM
301
C
GLN
38
137.192
47.285
56.860
1.00
23.61
L


ATOM
302
O
GLN
38
137.952
47.630
55.963
1.00
30.06
L


ATOM
303
N
LYS
39
137.610
46.969
58.086
1.00
29.96
L


ATOM
304
CA
LYS
39
139.021
47.022
58.464
1.00
20.79
L


ATOM
305
CB
LYS
39
139.204
46.606
59.925
1.00
26.60
L


ATOM
306
CG
LYS
39
139.493
47.764
60.884
1.00
31.56
L


ATOM
307
CD
LYS
39
138.268
48.135
61.727
1.00
24.64
L


ATOM
308
CE
LYS
39
138.633
48.266
63.205
1.00
28.08
L


ATOM
309
NZ
LYS
39
137.922
47.293
64.094
1.00
32.42
L


ATOM
310
C
LYS
39
139.831
46.103
57.566
1.00
39.66
L


ATOM
311
O
LYS
39
139.272
45.238
56.886
1.00
49.56
L


ATOM
312
N
PRO
40
141.161
46.283
57.533
1.00
54.59
L


ATOM
313
CD
PRO
40
141.957
47.289
58.266
1.00
52.22
L


ATOM
314
CA
PRO
40
142.007
45.434
56.686
1.00
54.43
L


ATOM
315
CB
PRO
40
143.431
45.874
57.037
1.00
40.87
L


ATOM
316
CG
PRO
40
143.274
47.280
57.530
1.00
36.77
L


ATOM
317
C
PRO
40
141.792
43.935
56.895
1.00
53.33
L


ATOM
318
O
PRO
40
141.962
43.429
58.003
1.00
48.18
L


ATOM
319
N
GLY
41
141.403
43.244
55.825
1.00
56.47
L


ATOM
320
CA
GLY
41
141.178
41.807
55.889
1.00
46.36
L


ATOM
321
C
GLY
41
140.030
41.378
56.783
1.00
47.51
L


ATOM
322
O
GLY
41
139.890
40.195
57.103
1.00
42.82
L


ATOM
323
N
ASN
42
139.198
42.336
57.177
1.00
39.14
L


ATOM
324
CA
ASN
42
138.061
42.049
58.044
1.00
33.16
L


ATOM
325
CB
ASN
42
138.031
43.041
59.210
1.00
25.23
L


ATOM
326
CG
ASN
42
137.400
42.453
60.451
1.00
34.65
L


ATOM
327
OD1
ASN
42
136.876
41.338
60.416
1.00
42.65
L


ATOM
328
ND2
ASN
42
137.444
43.194
61.558
1.00
15.22
L


ATOM
329
C
ASN
42
136.743
42.123
57.287
1.00
36.43
L


ATOM
330
O
ASN
42
136.726
42.413
56.092
1.00
31.10
L


ATOM
331
N
ILE
43
135.648
41.852
57.993
1.00
46.04
L


ATOM
332
CA
ILE
43
134.310
41.894
57.407
1.00
55.78
L


ATOM
333
CB
ILE
43
133.340
40.879
58.089
1.00
55.57
L


ATOM
334
CG2
ILE
43
134.038
39.554
58.323
1.00
47.83
L


ATOM
335
CG1
ILE
43
132.828
41.439
59.423
1.00
61.00
L


ATOM
336
CD1
ILE
43
131.656
40.662
60.017
1.00
68.36
L


ATOM
337
C
ILE
43
133.726
43.298
57.555
1.00
63.83
L


ATOM
338
O
ILE
43
134.037
44.010
58.513
1.00
72.13
L


ATOM
339
N
PRO
44
132.881
43.720
56.605
1.00
56.85
L


ATOM
340
CD
PRO
44
132.444
42.988
55.402
1.00
59.76
L


ATOM
341
CA
PRO
44
132.282
45.052
56.681
1.00
47.82
L


ATOM
342
CB
PRO
44
131.893
45.349
55.242
1.00
52.95
L


ATOM
343
CG
PRO
44
131.590
43.997
54.665
1.00
66.17
L


ATOM
344
C
PRO
44
131.079
45.064
57.613
1.00
41.70
L


ATOM
345
O
PRO
44
130.160
44.258
57.460
1.00
35.22
L


ATOM
346
N
LYS
45
131.095
45.982
58.575
1.00
33.96
L


ATOM
347
CA
LYS
45
130.010
46.106
59.537
1.00
39.65
L


ATOM
348
CB
LYS
45
130.576
46.225
60.953
1.00
36.05
L


ATOM
349
CG
LYS
45
132.096
46.222
61.012
1.00
44.96
L


ATOM
350
CD
LYS
45
132.651
45.003
61.752
1.00
53.54
L


ATOM
351
CE
LYS
45
134.060
45.269
62.297
1.00
58.35
L


ATOM
352
NZ
LYS
45
134.818
44.011
62.586
1.00
46.04
L


ATOM
353
C
LYS
45
129.162
47.329
59.218
1.00
37.07
L


ATOM
354
O
LYS
45
129.686
48.369
58.817
1.00
37.21
L


ATOM
355
N
LEU
46
127.850
47.205
59.385
1.00
22.26
L


ATOM
356
CA
LEU
46
126.969
48.326
59.107
1.00
28.40
L


ATOM
357
CB
LEU
46
125.505
47.904
59.239
1.00
32.99
L


ATOM
358
CG
LEU
46
124.429
48.756
58.541
1.00
40.56
L


ATOM
359
CD1
LEU
46
123.264
48.947
59.508
1.00
53.21
L


ATOM
360
CD2
LEU
46
124.987
50.114
58.096
1.00
41.53
L


ATOM
361
C
LEU
46
127.268
49.459
60.079
1.00
27.28
L


ATOM
362
O
LEU
46
127.353
49.247
61.286
1.00
40.74
L


ATOM
363
N
LEU
47
127.414
50.666
59.548
1.00
31.54
L


ATOM
364
CA
LEU
47
127.719
51.837
60.362
1.00
26.31
L


ATOM
365
CB
LEU
47
128.915
52.562
59.755
1.00
25.09
L


ATOM
366
CG
LEU
47
130.100
52.926
60.636
1.00
16.21
L


ATOM
367
CD1
LEU
47
130.727
51.676
61.198
1.00
17.63
L


ATOM
368
CD2
LEU
47
131.094
53.701
59.805
1.00
2.00
L


ATOM
369
C
LEU
47
126.535
52.807
60.449
1.00
34.89
L


ATOM
370
O
LEU
47
126.190
53.282
61.535
1.00
26.31
L


ATOM
371
N
ILE
48
125.934
53.100
59.293
1.00
44.65
L


ATOM
372
CA
ILE
48
124.794
54.018
59.187
1.00
34.33
L


ATOM
373
CB
ILE
48
125.251
55.439
58.788
1.00
24.64
L


ATOM
374
CG2
ILE
48
124.145
56.146
58.008
1.00
29.43
L


ATOM
375
CG1
ILE
48
125.591
56.253
60.033
1.00
28.98
L


ATOM
376
CD1
ILE
48
126.967
56.866
59.993
1.00
25.03
L


ATOM
377
C
ILE
48
123.775
53.576
58.133
1.00
29.31
L


ATOM
378
O
ILE
48
124.113
53.411
56.962
1.00
40.59
L


ATOM
379
N
TYR
49
122.527
53.405
58.547
1.00
15.91
L


ATOM
380
CA
TYR
49
121.485
53.018
57.613
1.00
21.27
L


ATOM
381
CB
TYR
49
120.762
51.748
58.087
1.00
36.34
L


ATOM
382
CG
TYR
49
119.779
51.925
59.231
1.00
35.16
L


ATOM
383
CD1
TYR
49
118.408
52.066
58.986
1.00
25.01
L


ATOM
384
CE1
TYR
49
117.498
52.216
60.028
1.00
28.72
L


ATOM
385
CD2
TYR
49
120.214
51.934
60.560
1.00
28.85
L


ATOM
386
CE2
TYR
49
119.308
52.084
61.614
1.00
30.37
L


ATOM
387
CZ
TYR
49
117.953
52.228
61.340
1.00
42.99
L


ATOM
388
OH
TYR
49
117.048
52.410
62.370
1.00
55.09
L


ATOM
389
C
TYR
49
120.510
54.172
57.486
1.00
24.72
L


ATOM
390
O
TYR
49
120.451
55.037
58.356
1.00
26.69
L


ATOM
391
N
LYS
50
119.762
54.189
56.392
1.00
25.34
L


ATOM
392
CA
LYS
50
118.786
55.241
56.132
1.00
19.61
L


ATOM
393
CB
LYS
50
117.611
55.134
57.108
1.00
42.59
L


ATOM
394
CG
LYS
50
116.426
54.345
56.566
1.00
36.91
L


ATOM
395
CD
LYS
50
115.124
55.118
56.723
1.00
40.28
L


ATOM
396
CE
LYS
50
114.289
54.568
57.865
1.00
33.03
L


ATOM
397
NZ
LYS
50
112.829
54.799
57.659
1.00
38.05
L


ATOM
398
C
LYS
50
119.368
56.645
56.192
1.00
22.01
L


ATOM
399
O
LYS
50
118.697
57.575
56.623
1.00
30.12
L


ATOM
400
N
ALA
51
120.622
56.790
55.779
1.00
32.92
L


ATOM
401
CA
ALA
51
121.275
58.094
55.749
1.00
27.76
L


ATOM
402
CB
ALA
51
120.346
59.117
55.100
1.00
14.79
L


ATOM
403
C
ALA
51
121.787
58.647
57.073
1.00
25.18
L


ATOM
404
O
ALA
51
122.949
59.026
57.173
1.00
28.58
L


ATOM
405
N
SER
52
120.927
58.714
58.081
1.00
22.15
L


ATOM
406
CA
SER
52
121.347
59.270
59.362
1.00
19.39
L


ATOM
407
CB
SER
52
120.690
60.632
59.599
1.00
25.09
L


ATOM
408
OG
SER
52
120.199
61.188
58.390
1.00
53.81
L


ATOM
409
C
SER
52
121.021
58.369
60.518
1.00
20.68
L


ATOM
410
O
SER
52
121.232
58.729
61.674
1.00
23.62
L


ATOM
411
N
ASN
53
120.497
57.194
60.209
1.00
25.87
L


ATOM
412
CA
ASN
53
120.157
56.253
61.254
1.00
27.39
L


ATOM
413
CB
ASN
53
118.992
55.387
60.811
1.00
27.54
L


ATOM
414
CG
ASN
53
117.668
55.895
61.323
1.00
28.84
L


ATOM
415
OD1
ASN
53
116.619
55.656
60.729
1.00
26.55
L


ATOM
416
ND2
ASN
53
117.713
56.607
62.450
1.00
36.37
L


ATOM
417
C
ASN
53
121.366
55.402
61.592
1.00
25.27
L


ATOM
418
O
ASN
53
121.809
54.592
60.783
1.00
34.60
L


ATOM
419
N
LEU
54
121.899
55.607
62.795
1.00
34.99
L


ATOM
420
CA
LEU
54
123.064
54.880
63.294
1.00
47.12
L


ATOM
421
CB
LEU
54
123.649
55.607
64.510
1.00
37.02
L


ATOM
422
CG
LEU
54
125.152
55.873
64.594
1.00
20.40
L


ATOM
423
CD1
LEU
54
125.508
57.117
63.830
1.00
25.30
L


ATOM
424
CD2
LEU
54
125.533
56.034
66.039
1.00
22.53
L


ATOM
425
C
LEU
54
122.660
53.468
63.705
1.00
56.63
L


ATOM
426
O
LEU
54
121.529
53.250
64.145
1.00
68.94
L


ATOM
427
N
HIS
55
123.578
52.513
63.573
1.00
51.79
L


ATOM
428
CA
HIS
55
123.267
51.142
63.948
1.00
38.57
L


ATOM
429
CB
HIS
55
124.091
50.139
63.137
1.00
36.54
L


ATOM
430
CG
HIS
55
123.551
48.742
63.186
1.00
27.93
L


ATOM
431
CD2
HIS
55
122.338
48.268
63.551
1.00
31.02
L


ATOM
432
ND1
HIS
55
124.303
47.639
62.841
1.00
36.74
L


ATOM
433
CE1
HIS
55
123.573
46.545
62.993
1.00
43.03
L


ATOM
434
NE2
HIS
55
122.376
46.902
63.423
1.00
37.77
L


ATOM
435
C
HIS
55
123.513
50.925
65.424
1.00
38.01
L


ATOM
436
O
HIS
55
124.296
51.634
66.058
1.00
47.97
L


ATOM
437
N
THR
56
122.832
49.930
65.966
1.00
33.15
L


ATOM
438
CA
THR
56
122.951
49.600
67.371
1.00
41.46
L


ATOM
439
CB
THR
56
122.008
48.436
67.708
1.00
58.72
L


ATOM
440
OG1
THR
56
121.601
47.787
66.495
1.00
57.81
L


ATOM
441
CG2
THR
56
120.770
48.955
68.430
1.00
63.75
L


ATOM
442
C
THR
56
124.380
49.260
67.805
1.00
32.47
L


ATOM
443
O
THR
56
124.940
48.236
67.415
1.00
23.36
L


ATOM
444
N
GLY
57
124.967
50.136
68.612
1.00
28.03
L


ATOM
445
CA
GLY
57
126.311
49.893
69.101
1.00
36.90
L


ATOM
446
C
GLY
57
127.388
50.703
68.403
1.00
48.73
L


ATOM
447
O
GLY
57
128.584
50.506
68.641
1.00
45.48
L


ATOM
448
N
VAL
58
126.971
51.616
67.537
1.00
45.32
L


ATOM
449
CA
VAL
58
127.926
52.439
66.819
1.00
42.70
L


ATOM
450
CB
VAL
58
127.448
52.680
65.372
1.00
41.53
L


ATOM
451
CG1
VAL
58
128.383
53.648
64.654
1.00
20.54
L


ATOM
452
CG2
VAL
58
127.380
51.354
64.635
1.00
16.05
L


ATOM
453
C
VAL
58
128.129
53.768
67.546
1.00
40.96
L


ATOM
454
O
VAL
58
127.178
54.509
67.780
1.00
35.53
L


ATOM
455
N
PRO
59
129.378
54.059
67.951
1.00
38.91
L


ATOM
456
CD
PRO
59
130.553
53.187
67.788
1.00
42.54
L


ATOM
457
CA
PRO
59
129.717
55.296
68.658
1.00
38.07
L


ATOM
458
CB
PRO
59
131.253
55.289
68.706
1.00
34.29
L


ATOM
459
CG
PRO
59
131.698
54.148
67.847
1.00
25.37
L


ATOM
460
C
PRO
59
129.174
56.543
67.980
1.00
36.88
L


ATOM
461
O
PRO
59
129.273
56.694
66.762
1.00
38.73
L


ATOM
462
N
SER
60
128.605
57.437
68.782
1.00
35.30
L


ATOM
463
CA
SER
60
128.044
58.680
68.274
1.00
37.70
L


ATOM
464
CB
SER
60
127.466
59.513
69.424
1.00
47.02
L


ATOM
465
OG
SER
60
128.108
59.201
70.648
1.00
44.15
L


ATOM
466
C
SER
60
129.096
59.492
67.536
1.00
33.70
L


ATOM
467
O
SER
60
128.760
60.405
66.785
1.00
38.05
L


ATOM
468
N
ARG
61
130.369
59.175
67.758
1.00
31.36
L


ATOM
469
CA
ARG
61
131.440
59.887
67.068
1.00
40.37
L


ATOM
470
CB
ARG
61
132.801
59.241
67.361
1.00
45.69
L


ATOM
471
CG
ARG
61
133.316
58.277
66.278
1.00
69.44
L


ATOM
472
CD
ARG
61
134.854
58.263
66.188
1.00
56.63
L


ATOM
473
NE
ARG
61
135.482
57.895
67.458
1.00
41.22
L


ATOM
474
CZ
ARG
61
135.733
56.646
67.834
1.00
36.39
L


ATOM
475
NH1
ARG
61
135.411
55.637
67.041
1.00
46.50
L


ATOM
476
NH2
ARG
61
136.294
56.405
69.008
1.00
45.10
L


ATOM
477
C
ARG
61
131.137
59.822
65.573
1.00
39.44
L


ATOM
478
O
ARG
61
131.495
60.718
64.807
1.00
31.59
L


ATOM
479
N
PHE
62
130.468
58.744
65.176
1.00
29.20
L


ATOM
480
CA
PHE
62
130.083
58.534
63.795
1.00
21.62
L


ATOM
481
CB
PHE
62
129.922
57.038
63.507
1.00
15.95
L


ATOM
482
CG
PHE
62
131.216
56.325
63.248
1.00
28.60
L


ATOM
483
CD1
PHE
62
131.770
56.303
61.975
1.00
25.81
L


ATOM
484
CD2
PHE
62
131.884
55.661
64.277
1.00
34.71
L


ATOM
485
CE1
PHE
62
132.973
55.629
61.730
1.00
25.28
L


ATOM
486
CE2
PHE
62
133.086
54.987
64.039
1.00
17.13
L


ATOM
487
CZ
PHE
62
133.628
54.972
62.759
1.00
16.17
L


ATOM
488
C
PHE
62
128.749
59.223
63.574
1.00
27.58
L


ATOM
489
O
PHE
62
127.812
59.041
64.347
1.00
26.02
L


ATOM
490
N
SER
63
128.667
60.028
62.526
1.00
27.01
L


ATOM
491
CA
SER
63
127.432
60.724
62.194
1.00
29.21
L


ATOM
492
CB
SER
63
127.512
62.197
62.620
1.00
27.06
L


ATOM
493
OG
SER
63
127.604
63.059
61.501
1.00
46.67
L


ATOM
494
C
SER
63
127.271
60.609
60.689
1.00
31.69
L


ATOM
495
O
SER
63
128.247
60.359
59.983
1.00
35.00
L


ATOM
496
N
GLY
64
126.046
60.778
60.203
1.00
32.44
L


ATOM
497
CA
GLY
64
125.796
60.677
58.775
1.00
28.03
L


ATOM
498
C
GLY
64
124.656
61.567
58.325
1.00
36.15
L


ATOM
499
O
GLY
64
123.678
61.753
59.050
1.00
31.20
L


ATOM
500
N
SER
65
124.761
62.103
57.115
1.00
45.47
L


ATOM
501
CA
SER
65
123.723
62.993
56.612
1.00
43.02
L


ATOM
502
CB
SER
65
124.029
64.412
57.075
1.00
39.26
L


ATOM
503
OG
SER
65
125.378
64.487
57.510
1.00
49.79
L


ATOM
504
C
SER
65
123.566
62.975
55.097
1.00
33.50
L


ATOM
505
O
SER
65
124.401
62.428
54.373
1.00
35.77
L


ATOM
506
N
GLY
66
122.485
63.579
54.625
1.00
17.16
L


ATOM
507
CA
GLY
66
122.258
63.641
53.196
1.00
29.10
L


ATOM
508
C
GLY
66
120.920
63.081
52.769
1.00
35.42
L


ATOM
509
O
GLY
66
120.321
62.267
53.479
1.00
32.46
L


ATOM
510
N
SER
67
120.449
63.527
51.608
1.00
24.62
L


ATOM
511
CA
SER
67
119.184
63.062
51.061
1.00
28.74
L


ATOM
512
CB
SER
67
118.012
63.757
51.757
1.00
46.75
L


ATOM
513
OG
SER
67
116.780
63.142
51.412
1.00
59.32
L


ATOM
514
C
SER
67
119.129
63.343
49.567
1.00
32.92
L


ATOM
515
O
SER
67
119.579
64.391
49.113
1.00
35.08
L


ATOM
516
N
GLY
68
118.589
62.399
48.803
1.00
32.00
L


ATOM
517
CA
GLY
68
118.485
62.585
47.367
1.00
22.78
L


ATOM
518
C
GLY
68
119.712
62.129
46.604
1.00
35.09
L


ATOM
519
O
GLY
68
119.885
60.935
46.341
1.00
32.80
L


ATOM
520
N
THR
69
120.567
63.086
46.255
1.00
36.67
L


ATOM
521
CA
THR
69
121.784
62.805
45.505
1.00
36.14
L


ATOM
522
CB
THR
69
121.890
63.748
44.285
1.00
43.08
L


ATOM
523
OG1
THR
69
120.775
63.516
43.414
1.00
47.12
L


ATOM
524
CG2
THR
69
123.175
63.505
43.514
1.00
44.41
L


ATOM
525
C
THR
69
123.029
62.953
46.376
1.00
29.87
L


ATOM
526
O
THR
69
123.915
62.101
46.350
1.00
38.58
L


ATOM
527
N
GLY
70
123.082
64.022
47.160
1.00
31.63
L


ATOM
528
CA
GLY
70
124.234
64.256
48.016
1.00
42.61
L


ATOM
529
C
GLY
70
124.171
63.521
49.337
1.00
36.70
L


ATOM
530
O
GLY
70
123.119
63.471
49.965
1.00
45.53
L


ATOM
531
N
PHE
71
125.295
62.944
49.755
1.00
38.83
L


ATOM
532
CA
PHE
71
125.370
62.206
51.019
1.00
33.73
L


ATOM
533
CB
PHE
71
124.999
60.739
50.798
1.00
31.54
L


ATOM
534
CG
PHE
71
123.602
60.540
50.263
1.00
38.58
L


ATOM
535
CD1
PHE
71
122.561
60.199
51.120
1.00
21.48
L


ATOM
536
CD2
PHE
71
123.329
60.697
48.908
1.00
26.22
L


ATOM
537
CE1
PHE
71
121.278
60.021
50.641
1.00
26.37
L


ATOM
538
CE2
PHE
71
122.044
60.520
48.422
1.00
42.61
L


ATOM
539
CZ
PHE
71
121.016
60.183
49.286
1.00
24.00
L


ATOM
540
C
PHE
71
126.774
62.296
51.605
1.00
27.97
L


ATOM
541
O
PHE
71
127.760
62.212
50.877
1.00
31.31
L


ATOM
542
N
THR
72
126.866
62.457
52.921
1.00
26.78
L


ATOM
543
CA
THR
72
128.167
62.582
53.564
1.00
26.67
L


ATOM
544
CB
THR
72
128.520
64.069
53.781
1.00
32.81
L


ATOM
545
OG1
THR
72
129.493
64.187
54.826
1.00
56.77
L


ATOM
546
CG2
THR
72
127.288
64.856
54.164
1.00
32.03
L


ATOM
547
C
THR
72
128.291
61.866
54.903
1.00
27.04
L


ATOM
548
O
THR
72
127.308
61.722
55.635
1.00
23.90
L


ATOM
549
N
LEU
73
129.511
61.417
55.207
1.00
31.55
L


ATOM
550
CA
LEU
73
129.816
60.722
56.459
1.00
24.92
L


ATOM
551
CB
LEU
73
130.387
59.333
56.194
1.00
32.89
L


ATOM
552
CG
LEU
73
130.625
58.551
57.490
1.00
34.57
L


ATOM
553
CD1
LEU
73
129.287
58.045
58.021
1.00
14.98
L


ATOM
554
CD2
LEU
73
131.586
57.404
57.246
1.00
10.86
L


ATOM
555
C
LEU
73
130.826
61.512
57.274
1.00
25.60
L


ATOM
556
O
LEU
73
131.823
62.004
56.755
1.00
39.03
L


ATOM
557
N
THR
74
130.581
61.593
58.567
1.00
21.07
L


ATOM
558
CA
THR
74
131.443
62.362
59.436
1.00
25.98
L


ATOM
559
CB
THR
74
130.686
63.625
59.933
1.00
34.99
L


ATOM
560
OG1
THR
74
130.181
64.351
58.808
1.00
35.85
L


ATOM
561
CG2
THR
74
131.588
64.524
60.747
1.00
27.11
L


ATOM
562
C
THR
74
131.886
61.542
60.640
1.00
29.95
L


ATOM
563
O
THR
74
131.065
60.931
61.322
1.00
36.77
L


ATOM
564
N
ILE
75
133.186
61.522
60.896
1.00
24.95
L


ATOM
565
CA
ILE
75
133.706
60.813
62.052
1.00
23.49
L


ATOM
566
CB
ILE
75
134.723
59.737
61.659
1.00
21.59
L


ATOM
567
CG2
ILE
75
135.597
59.401
62.847
1.00
7.06
L


ATOM
568
CG1
ILE
75
133.988
58.479
61.198
1.00
33.47
L


ATOM
569
CD1
ILE
75
133.840
58.343
59.687
1.00
25.94
L


ATOM
570
C
ILE
75
134.399
61.855
62.914
1.00
34.16
L


ATOM
571
O
ILE
75
135.440
62.381
62.537
1.00
28.63
L


ATOM
572
N
SER
76
133.803
62.167
64.059
1.00
42.00
L


ATOM
573
CA
SER
76
134.366
63.155
64.971
1.00
51.91
L


ATOM
574
CB
SER
76
133.251
63.762
65.829
1.00
67.85
L


ATOM
575
OG
SER
76
131.981
63.244
65.449
1.00
75.08
L


ATOM
576
C
SER
76
135.424
62.507
65.865
1.00
55.79
L


ATOM
577
O
SER
76
135.372
61.304
66.131
1.00
54.31
L


ATOM
578
N
SER
77
136.384
63.307
66.319
1.00
51.60
L


ATOM
579
CA
SER
77
137.459
62.818
67.177
1.00
51.14
L


ATOM
580
CB
SER
77
136.993
62.788
68.633
1.00
51.40
L


ATOM
581
OG
SER
77
136.336
63.998
68.967
1.00
52.89
L


ATOM
582
C
SER
77
137.938
61.433
66.755
1.00
49.10
L


ATOM
583
O
SER
77
137.515
60.422
67.311
1.00
47.48
L


ATOM
584
N
LEU
78
138.832
61.406
65.771
1.00
46.54
L


ATOM
585
CA
LEU
78
139.389
60.168
65.241
1.00
32.83
L


ATOM
586
CB
LEU
78
140.333
60.495
64.084
1.00
27.78
L


ATOM
587
CG
LEU
78
140.423
59.481
62.950
1.00
35.44
L


ATOM
588
CD1
LEU
78
139.472
59.898
61.855
1.00
40.91
L


ATOM
589
CD2
LEU
78
141.852
59.397
62.421
1.00
25.57
L


ATOM
590
C
LEU
78
140.134
59.314
66.268
1.00
28.27
L


ATOM
591
O
LEU
78
140.668
59.809
67.264
1.00
19.55
L


ATOM
592
N
GLN
79
140.163
58.016
65.999
1.00
30.85
L


ATOM
593
CA
GLN
79
140.847
57.064
66.859
1.00
34.63
L


ATOM
594
CB
GLN
79
139.855
56.338
67.763
1.00
47.40
L


ATOM
595
CG
GLN
79
139.162
57.238
68.764
1.00
66.32
L


ATOM
596
CD
GLN
79
140.118
57.793
69.791
1.00
51.70
L


ATOM
597
OE1
GLN
79
141.184
57.229
70.022
1.00
38.18
L


ATOM
598
NE2
GLN
79
139.740
58.901
70.419
1.00
50.52
L


ATOM
599
C
GLN
79
141.534
56.054
65.965
1.00
36.58
L


ATOM
600
O
GLN
79
141.056
55.755
64.869
1.00
30.40
L


ATOM
601
N
PRO
80
142.663
55.504
66.430
1.00
40.78
L


ATOM
602
CD
PRO
80
143.270
55.791
67.740
1.00
37.68
L


ATOM
603
CA
PRO
80
143.430
54.513
65.665
1.00
41.10
L


ATOM
604
CB
PRO
80
144.460
53.991
66.672
1.00
43.39
L


ATOM
605
CG
PRO
80
144.056
54.557
68.021
1.00
37.64
L


ATOM
606
C
PRO
80
142.532
53.398
65.149
1.00
46.35
L


ATOM
607
O
PRO
80
142.569
53.048
63.969
1.00
41.86
L


ATOM
608
N
GLU
81
141.721
52.859
66.056
1.00
57.27
L


ATOM
609
CA
GLU
81
140.793
51.773
65.761
1.00
58.29
L


ATOM
610
CB
GLU
81
139.870
51.548
66.967
1.00
54.76
L


ATOM
611
CG
GLU
81
138.677
52.498
67.042
1.00
71.46
L


ATOM
612
CD
GLU
81
138.507
53.125
68.419
1.00
74.52
L


ATOM
613
OE1
GLU
81
137.390
53.039
68.983
1.00
60.52
L


ATOM
614
OE2
GLU
81
139.489
53.703
68.934
1.00
65.39
L


ATOM
615
C
GLU
81
139.956
51.998
64.503
1.00
62.35
L


ATOM
616
O
GLU
81
139.618
51.052
63.794
1.00
64.51
L


ATOM
617
N
ASP
82
139.629
53.256
64.230
1.00
63.68
L


ATOM
618
CA
ASP
82
138.823
53.600
63.069
1.00
50.22
L


ATOM
619
CB
ASP
82
138.299
55.024
63.212
1.00
50.14
L


ATOM
620
CG
ASP
82
137.729
55.285
64.581
1.00
50.17
L


ATOM
621
OD1
ASP
82
137.107
54.358
65.143
1.00
56.15
L


ATOM
622
OD2
ASP
82
137.906
56.408
65.095
1.00
62.15
L


ATOM
623
C
ASP
82
139.583
53.462
61.761
1.00
47.40
L


ATOM
624
O
ASP
82
139.080
53.837
60.706
1.00
44.58
L


ATOM
625
N
ILE
83
140.801
52.935
61.830
1.00
43.70
L


ATOM
626
CA
ILE
83
141.586
52.738
60.621
1.00
38.44
L


ATOM
627
CB
ILE
83
142.991
52.194
60.932
1.00
41.83
L


ATOM
628
CG2
ILE
83
143.428
51.225
59.846
1.00
41.86
L


ATOM
629
CG1
ILE
83
143.982
53.356
61.025
1.00
52.87
L


ATOM
630
CD1
ILE
83
145.232
53.032
61.826
1.00
47.10
L


ATOM
631
C
ILE
83
140.827
51.706
59.794
1.00
32.55
L


ATOM
632
O
ILE
83
140.588
50.594
60.263
1.00
26.83
L


ATOM
633
N
ALA
84
140.441
52.085
58.575
1.00
26.12
L


ATOM
634
CA
ALA
84
139.692
51.200
57.693
1.00
15.10
L


ATOM
635
CB
ALA
84
138.453
50.702
58.406
1.00
21.07
L


ATOM
636
C
ALA
84
139.290
51.913
56.414
1.00
12.91
L


ATOM
637
O
ALA
84
139.668
53.060
56.195
1.00
24.44
L


ATOM
638
N
THR
85
138.519
51.224
55.578
1.00
19.66
L


ATOM
639
CA
THR
85
138.025
51.774
54.320
1.00
17.95
L


ATOM
640
CB
THR
85
138.413
50.898
53.131
1.00
21.81
L


ATOM
641
OG1
THR
85
137.552
51.197
52.024
1.00
45.12
L


ATOM
642
CG2
THR
85
138.277
49.435
53.490
1.00
27.37
L


ATOM
643
C
THR
85
136.505
51.809
54.410
1.00
27.74
L


ATOM
644
O
THR
85
135.876
50.804
54.732
1.00
32.43
L


ATOM
645
N
TYR
86
135.914
52.960
54.116
1.00
37.25
L


ATOM
646
CA
TYR
86
134.469
53.109
54.213
1.00
33.34
L


ATOM
647
CB
TYR
86
134.153
54.363
55.028
1.00
25.32
L


ATOM
648
CG
TYR
86
134.852
54.381
56.372
1.00
28.51
L


ATOM
649
CD1
TYR
86
134.151
54.118
57.547
1.00
44.83
L


ATOM
650
CE1
TYR
86
134.801
54.071
58.789
1.00
21.36
L


ATOM
651
CD2
TYR
86
136.226
54.607
56.467
1.00
14.36
L


ATOM
652
CE2
TYR
86
136.884
54.561
57.705
1.00
22.98
L


ATOM
653
CZ
TYR
86
136.158
54.289
58.857
1.00
22.24
L


ATOM
654
OH
TYR
86
136.786
54.210
60.072
1.00
25.93
L


ATOM
655
C
TYR
86
133.766
53.159
52.868
1.00
44.00
L


ATOM
656
O
TYR
86
134.164
53.908
51.975
1.00
50.72
L


ATOM
657
N
TYR
87
132.712
52.353
52.748
1.00
51.03
L


ATOM
658
CA
TYR
87
131.910
52.248
51.526
1.00
48.37
L


ATOM
659
CB
TYR
87
131.795
50.780
51.108
1.00
25.20
L


ATOM
660
CG
TYR
87
133.108
50.126
50.753
1.00
41.42
L


ATOM
661
CD1
TYR
87
133.551
50.080
49.427
1.00
38.02
L


ATOM
662
CE1
TYR
87
134.769
49.484
49.096
1.00
42.21
L


ATOM
663
CD2
TYR
87
133.918
49.555
51.742
1.00
33.37
L


ATOM
664
CE2
TYR
87
135.137
48.957
51.418
1.00
33.81
L


ATOM
665
CZ
TYR
87
135.555
48.929
50.096
1.00
23.95
L


ATOM
666
OH
TYR
87
136.771
48.380
49.779
1.00
34.33
L


ATOM
667
C
TYR
87
130.493
52.813
51.687
1.00
52.12
L


ATOM
668
O
TYR
87
129.948
52.838
52.786
1.00
65.19
L


ATOM
669
N
CYS
88
129.895
53.250
50.582
1.00
47.34
L


ATOM
670
CA
CYS
88
128.538
53.789
50.598
1.00
32.49
L


ATOM
671
C
CYS
88
127.715
52.920
49.661
1.00
32.31
L


ATOM
672
O
CYS
88
128.140
52.641
48.547
1.00
39.98
L


ATOM
673
CB
CYS
88
128.530
55.233
50.093
1.00
33.98
L


ATOM
674
SG
CYS
88
128.799
55.411
48.289
1.00
51.95
L


ATOM
675
N
GLN
89
126.549
52.476
50.109
1.00
22.59
L


ATOM
676
CA
GLN
89
125.709
51.648
49.260
1.00
21.84
L


ATOM
677
CB
GLN
89
125.810
50.179
49.700
1.00
31.57
L


ATOM
678
CG
GLN
89
124.788
49.740
50.735
1.00
42.44
L


ATOM
679
CD
GLN
89
123.613
48.982
50.132
1.00
34.76
L


ATOM
680
OE1
GLN
89
122.664
48.637
50.834
1.00
26.74
L


ATOM
681
NE2
GLN
89
123.671
48.723
48.831
1.00
46.97
L


ATOM
682
C
GLN
89
124.262
52.131
49.287
1.00
25.45
L


ATOM
683
O
GLN
89
123.783
52.603
50.313
1.00
20.31
L


ATOM
684
N
GLN
90
123.574
52.035
48.153
1.00
19.13
L


ATOM
685
CA
GLN
90
122.183
52.457
48.096
1.00
19.46
L


ATOM
686
CB
GLN
90
121.882
53.162
46.769
1.00
22.24
L


ATOM
687
CG
GLN
90
122.072
52.299
45.523
1.00
38.10
L


ATOM
688
CD
GLN
90
120.775
51.666
45.009
1.00
30.31
L


ATOM
689
OE1
GLN
90
119.701
51.853
45.586
1.00
32.81
L


ATOM
690
NE2
GLN
90
120.878
50.908
43.921
1.00
28.00
L


ATOM
691
C
GLN
90
121.257
51.250
48.267
1.00
30.72
L


ATOM
692
O
GLN
90
121.556
50.143
47.801
1.00
25.46
L


ATOM
693
N
GLY
91
120.137
51.473
48.951
1.00
34.63
L


ATOM
694
CA
GLY
91
119.166
50.416
49.179
1.00
33.97
L


ATOM
695
C
GLY
91
117.820
50.781
48.580
1.00
38.48
L


ATOM
696
O
GLY
91
116.762
50.335
49.035
1.00
28.43
L


ATOM
697
N
GLN
92
117.869
51.603
47.540
1.00
28.95
L


ATOM
698
CA
GLN
92
116.668
52.043
46.868
1.00
29.94
L


ATOM
699
CB
GLN
92
116.841
53.489
46.418
1.00
30.37
L


ATOM
700
CG
GLN
92
115.571
54.307
46.498
1.00
39.02
L


ATOM
701
CD
GLN
92
115.079
54.736
45.130
1.00
41.59
L


ATOM
702
OE1
GLN
92
114.039
54.274
44.663
1.00
31.97
L


ATOM
703
NE2
GLN
92
115.829
55.621
44.479
1.00
59.08
L


ATOM
704
C
GLN
92
116.352
51.144
45.680
1.00
41.66
L


ATOM
705
O
GLN
92
115.495
50.267
45.782
1.00
33.85
L


ATOM
706
N
THR
93
117.046
51.373
44.564
1.00
60.13
L


ATOM
707
CA
THR
93
116.866
50.596
43.327
1.00
66.93
L


ATOM
708
CB
THR
93
117.639
51.242
42.149
1.00
78.05
L


ATOM
709
OG1
THR
93
117.362
52.647
42.104
1.00
79.52
L


ATOM
710
CG2
THR
93
117.233
50.610
40.829
1.00
75.70
L


ATOM
711
C
THR
93
117.391
49.180
43.539
1.00
59.06
L


ATOM
712
O
THR
93
118.425
49.009
44.183
1.00
58.37
L


ATOM
713
N
TYR
94
116.703
48.173
42.993
1.00
50.92
L


ATOM
714
CA
TYP
94
117.127
46.791
43.209
1.00
52.81
L


ATOM
715
CB
TYR
94
116.119
45.793
42.646
1.00
55.69
L


ATOM
716
CG
TYR
94
115.809
44.706
43.658
1.00
64.81
L


ATOM
717
CD1
TYR
94
116.742
44.361
44.643
1.00
66.44
L


ATOM
718
CE1
TYR
94
116.451
43.413
45.622
1.00
61.74
L


ATOM
719
CD2
TYR
94
114.573
44.066
43.676
1.00
65.08
L


ATOM
720
CE2
TYR
94
114.269
43.112
44.654
1.00
75.90
L


ATOM
721
CZ
TYR
94
115.214
42.796
45.623
1.00
73.24
L


ATOM
722
OH
TYR
94
114.918
41.877
46.603
1.00
71.00
L


ATOM
723
C
TYR
94
118.530
46.372
42.797
1.00
58.78
L


ATOM
724
O
TYR
94
119.146
45.561
43.487
1.00
72.46
L


ATOM
725
N
PRO
95
119.045
46.851
41.650
1.00
50.22
L


ATOM
726
CD
PRO
95
118.550
47.719
40.573
1.00
29.17
L


ATOM
727
CA
PRO
95
120.412
46.375
41.404
1.00
45.68
L


ATOM
728
CB
PRO
95
120.673
46.762
39.946
1.00
19.42
L


ATOM
729
CG
PRO
95
119.780
47.938
39.712
1.00
25.27
L


ATOM
730
C
PRO
95
121.286
47.156
42.398
1.00
43.53
L


ATOM
731
O
PRO
95
121.936
48.131
42.027
1.00
52.07
L


ATOM
732
N
TYR
96
121.253
46.734
43.667
1.00
32.53
L


ATOM
733
CA
TYR
96
122.000
47.378
44.748
1.00
29.95
L


ATOM
734
CB
TYR
96
122.099
46.463
45.971
1.00
35.33
L


ATOM
735
CG
TYR
96
120.833
46.317
46.792
1.00
28.60
L


ATOM
736
CD1
TYR
96
120.678
45.244
47.663
1.00
33.58
L


ATOM
737
CE1
TYR
96
119.523
45.087
48.430
1.00
23.02
L


ATOM
738
CD2
TYR
96
119.792
47.244
46.701
1.00
29.21
L


ATOM
739
CE2
TYR
96
118.625
47.096
47.466
1.00
29.26
L


ATOM
740
CZ
TYR
96
118.506
46.011
48.329
1.00
29.92
L


ATOM
741
OH
TYR
96
117.383
45.853
49.102
1.00
25.77
L


ATOM
742
C
TYR
96
123.392
47.712
44.281
1.00
29.68
L


ATOM
743
O
TYR
96
123.980
46.954
43.511
1.00
37.11
L


ATOM
744
N
THR
97
123.926
48.830
44.770
1.00
36.89
L


ATOM
745
CA
THR
97
125.259
49.280
44.372
1.00
40.63
L


ATOM
746
CB
THR
97
125.153
50.181
43.141
1.00
46.47
L


ATOM
747
OG1
THR
97
123.945
50.951
43.228
1.00
49.45
L


ATOM
748
CG2
THR
97
125.123
49.342
41.874
1.00
48.22
L


ATOM
749
C
THR
97
126.017
50.049
45.458
1.00
34.47
L


ATOM
750
O
THR
97
125.473
50.975
46.062
1.00
27.32
L


ATOM
751
N
PHE
98
127.268
49.662
45.700
1.00
24.58
L


ATOM
752
CA
PHE
98
128.097
50.338
46.689
1.00
19.63
L


ATOM
753
CB
PHE
98
129.009
49.369
47.458
1.00
21.69
L


ATOM
754
CG
PHE
98
128.386
48.051
47.780
1.00
22.09
L


ATOM
755
CD1
PHE
98
128.078
47.719
49.098
1.00
28.14
L


ATOM
756
CD2
PHE
98
128.145
47.117
46.780
1.00
29.87
L


ATOM
757
CE1
PHE
98
127.543
46.481
49.409
1.00
20.31
L


ATOM
758
CE2
PHE
98
127.610
45.877
47.080
1.00
20.97
L


ATOM
759
CZ
PHE
98
127.308
45.556
48.397
1.00
35.93
L


ATOM
760
C
PHE
98
128.994
51.317
45.947
1.00
27.49
L


ATOM
761
O
PHE
98
128.868
51.493
44.738
1.00
15.09
L


ATOM
762
N
GLY
99
129.912
51.931
46.689
1.00
32.03
L


ATOM
763
CA
GLY
99
130.839
52.878
46.106
1.00
37.27
L


ATOM
764
C
GLY
99
132.207
52.250
45.927
1.00
44.52
L


ATOM
765
O
GLY
99
132.319
51.038
45.717
1.00
42.71
L


ATOM
766
N
GLY
100
133.246
53.077
46.008
1.00
42.44
L


ATOM
767
CA
GLY
100
134.600
52.584
45.848
1.00
51.35
L


ATOM
768
C
GLY
100
135.344
52.448
47.164
1.00
53.12
L


ATOM
769
O
GLY
100
136.390
51.791
47.241
1.00
49.35
L


ATOM
770
N
GLY
101
134.799
53.067
48.204
1.00
43.66
L


ATOM
771
CA
GLY
101
135.430
53.013
49.504
1.00
26.42
L


ATOM
772
C
GLY
101
136.368
54.181
49.696
1.00
21.78
L


ATOM
773
O
GLY
101
136.710
54.886
48.747
1.00
23.28
L


ATOM
774
N
THR
102
136.787
54.392
50.933
1.00
17.65
L


ATOM
775
CA
THR
102
137.682
55.489
51.221
1.00
39.10
L


ATOM
776
CB
THR
102
136.869
56.745
51.644
1.00
49.68
L


ATOM
777
OG1
THR
102
137.738
57.731
52.224
1.00
42.53
L


ATOM
778
CG2
THR
102
135.780
56.357
52.630
1.00
55.18
L


ATOM
779
C
THR
102
138.659
55.072
52.311
1.00
37.54
L


ATOM
780
O
THR
102
138.295
54.948
53.478
1.00
44.36
L


ATOM
781
N
LYS
103
139.903
54.830
51.911
1.00
34.42
L


ATOM
782
CA
LYS
103
140.943
54.427
52.850
1.00
33.42
L


ATOM
783
CB
LYS
103
142.245
54.118
52.095
1.00
27.82
L


ATOM
784
CG
LYS
103
143.518
54.210
52.937
1.00
32.59
L


ATOM
785
CD
LYS
103
144.038
55.649
53.036
1.00
39.07
L


ATOM
786
CE
LYS
103
145.366
55.734
53.790
1.00
45.98
L


ATOM
787
NZ
LYS
103
146.255
56.817
53.258
1.00
37.43
L


ATOM
788
C
LYS
103
141.185
55.531
53.868
1.00
23.38
L


ATOM
789
O
LYS
103
140.842
56.687
53.637
1.00
30.72
L


ATOM
790
N
LEU
104
141.770
55.171
55.002
1.00
32.38
L


ATOM
791
CA
LEU
104
142.077
56.159
56.021
1.00
38.59
L


ATOM
792
CB
LEU
104
140.904
56.368
56.966
1.00
25.66
L


ATOM
793
CG
LEU
104
141.378
57.265
58.113
1.00
32.02
L


ATOM
794
CD1
LEU
104
140.804
58.658
57.944
1.00
23.79
L


ATOM
795
CD2
LEU
104
140.996
56.648
59.452
1.00
29.54
L


ATOM
796
C
LEU
104
143.300
55.784
56.841
1.00
45.89
L


ATOM
797
O
LEU
104
143.525
54.612
57.154
1.00
45.41
L


ATOM
798
N
GLU
105
144.080
56.799
57.197
1.00
40.34
L


ATOM
799
CA
GLU
105
145.280
56.597
57.979
1.00
35.75
L


ATOM
800
CB
GLU
105
146.510
56.674
57.064
1.00
52.11
L


ATOM
801
CG
GLU
105
147.798
57.154
57.728
1.00
69.87
L


ATOM
802
CD
GLU
105
148.876
57.513
56.712
1.00
84.87
L


ATOM
803
OE1
GLU
105
150.027
57.054
56.875
1.00
87.68
L


ATOM
804
OE2
GLU
105
148.578
58.251
55.748
1.00
91.43
L


ATOM
805
C
GLU
105
145.365
57.644
59.076
1.00
23.69
L


ATOM
806
O
GLU
105
145.640
58.804
58.808
1.00
36.93
L


ATOM
807
N
ILE
106
145.088
57.241
60.311
1.00
29.41
L


ATOM
808
CA
ILE
106
145.199
58.168
61.425
1.00
38.27
L


ATOM
809
CB
ILE
106
144.723
57.513
62.769
1.00
40.94
L


ATOM
810
CG2
ILE
106
145.030
56.026
62.766
1.00
31.48
L


ATOM
811
CG1
ILE
106
145.387
58.193
63.971
1.00
37.37
L


ATOM
812
CD1
ILE
106
144.571
59.336
64.579
1.00
27.37
L


ATOM
813
C
ILE
106
146.702
58.441
61.434
1.00
40.57
L


ATOM
814
O
ILE
106
147.497
57.506
61.438
1.00
41.72
L


ATOM
815
N
LYS
107
147.088
59.716
61.399
1.00
48.16
L


ATOM
816
CA
LYS
107
148.500
60.100
61.364
1.00
42.60
L


ATOM
817
CB
LYS
107
148.640
61.486
60.736
1.00
20.26
L


ATOM
818
CG
LYS
107
150.043
62.069
60.819
1.00
43.13
L


ATOM
819
CD
LYS
107
150.034
63.572
60.590
1.00
34.99
L


ATOM
820
CE
LYS
107
149.967
63.895
59.108
1.00
38.89
L


ATOM
821
NZ
LYS
107
148.581
63.791
58.588
1.00
13.28
L


ATOM
822
C
LYS
107
149.186
60.089
62.729
1.00
51.65
L


ATOM
823
O
LYS
107
148.643
60.592
63.713
1.00
65.04
L


ATOM
824
N
ARG
108
150.388
59.519
62.781
1.00
52.66
L


ATOM
825
CA
ARG
108
151.147
59.450
64.028
1.00
39.90
L


ATOM
826
CB
ARG
108
151.199
58.007
64.553
1.00
33.40
L


ATOM
827
CG
ARG
108
152.127
57.083
63.771
1.00
28.54
L


ATOM
828
CD
ARG
108
152.649
55.957
64.635
1.00
25.73
L


ATOM
829
NE
ARG
108
153.934
56.297
65.246
1.00
34.47
L


ATOM
830
CZ
ARG
108
154.303
55.928
66.471
1.00
34.06
L


ATOM
831
NH1
ARG
108
153.489
55.205
67.224
1.00
57.79
L


ATOM
832
NH2
ARG
108
155.484
56.280
66.947
1.00
31.28
L


ATOM
833
C
ARG
108
152.566
59.974
63.858
1.00
41.80
L


ATOM
834
O
ARG
108
153.071
60.102
62.733
1.00
36.52
L


ATOM
835
N
ALA
109
153.193
60.271
64.996
1.00
49.39
L


ATOM
836
CA
ALA
109
154.557
60.774
65.040
1.00
56.62
L


ATOM
837
CB
ALA
109
155.042
60.822
66.479
1.00
33.22
L


ATOM
838
C
ALA
109
155.479
59.894
64.209
1.00
68.35
L


ATOM
839
O
ALA
109
155.350
58.667
64.213
1.00
79.99
L


ATOM
840
N
ASP
110
156.403
60.524
63.492
1.00
75.21
L


ATOM
841
CA
ASP
110
157.350
59.784
62.673
1.00
65.78
L


ATOM
842
CB
ASP
110
158.395
60.740
62.082
1.00
68.55
L


ATOM
843
CG
ASP
110
157.861
61.528
60.889
1.00
84.17
L


ATOM
844
OD1
ASP
110
156.656
61.399
60.580
1.00
78.11
L


ATOM
845
OD2
ASP
110
158.641
62.277
60.256
1.00
94.16
L


ATOM
846
C
ASP
110
158.022
58.731
63.551
1.00
60.38
L


ATOM
847
O
ASP
110
158.093
58.882
64.776
1.00
54.97
L


ATOM
848
N
ALA
111
158.487
57.651
62.932
1.00
55.72
L


ATOM
849
CA
ALA
111
159.151
56.577
63.661
1.00
59.37
L


ATOM
850
CB
ALA
111
158.123
55.633
64.272
1.00
55.98
L


ATOM
851
C
ALA
111
160.074
55.815
62.730
1.00
51.95
L


ATOM
852
O
ALA
111
159.669
55.363
61.658
1.00
59.98
L


ATOM
853
N
ALA
112
161.328
55.685
63.141
1.00
47.00
L


ATOM
854
CA
ALA
112
162.318
54.977
62.348
1.00
42.20
L


ATOM
855
CB
ALA
112
163.712
55.266
62.887
1.00
43.77
L


ATOM
856
C
ALA
112
162.043
53.476
62.376
1.00
42.23
L


ATOM
857
O
ALA
112
161.447
52.957
63.325
1.00
45.43
L


ATOM
858
N
PRO
113
162.464
52.761
61.325
1.00
51.11
L


ATOM
859
CD
PRO
113
163.153
53.256
60.117
1.00
52.49
L


ATOM
860
CA
PRO
113
162.250
51.316
61.269
1.00
49.74
L


ATOM
861
CB
PRO
113
162.267
51.018
59.776
1.00
32.76
L


ATOM
862
CG
PRO
113
163.223
52.029
59.220
1.00
41.64
L


ATOM
863
C
PRO
113
163.356
50.568
61.997
1.00
45.33
L


ATOM
864
O
PRO
113
164.511
50.988
61.974
1.00
53.16
L


ATOM
865
N
THR
114
163.006
49.475
62.661
1.00
36.71
L


ATOM
866
CA
THR
114
164.009
48.675
63.341
1.00
39.49
L


ATOM
867
CB
THR
114
163.505
48.159
64.706
1.00
34.54
L


ATOM
868
OG1
THR
114
162.504
47.153
64.511
1.00
38.54
L


ATOM
869
CG2
THR
114
162.926
49.305
65.518
1.00
31.63
L


ATOM
870
C
THR
114
164.322
47.515
62.406
1.00
42.88
L


ATOM
871
O
THR
114
163.527
46.585
62.247
1.00
35.53
L


ATOM
872
N
VAL
115
165.488
47.596
61.769
1.00
36.33
L


ATOM
873
CA
VAL
115
165.939
46.594
60.815
1.00
42.49
L


ATOM
874
CB
VAL
115
166.973
47.210
59.839
1.00
46.96
L


ATOM
875
CG1
VAL
115
167.217
46.269
58.670
1.00
26.72
L


ATOM
876
CG2
VAL
115
166.470
48.555
59.338
1.00
30.46
L


ATOM
877
C
VAL
115
166.544
45.324
61.424
1.00
38.71
L


ATOM
878
O
VAL
115
167.064
45.327
62.541
1.00
36.49
L


ATOM
879
N
SER
116
166.458
44.237
60.659
1.00
44.76
L


ATOM
880
CA
SER
116
166.988
42.939
61.053
1.00
51.12
L


ATOM
881
CB
SER
116
165.975
42.188
61.913
1.00
55.78
L


ATOM
882
OG
SER
116
165.653
42.932
63.068
1.00
58.23
L


ATOM
883
C
SER
116
167.292
42.130
59.799
1.00
44.61
L


ATOM
884
O
SER
116
166.413
41.891
58.976
1.00
53.07
L


ATOM
885
N
ILE
117
168.547
41.726
59.641
1.00
40.79
L


ATOM
886
CA
ILE
117
168.935
40.929
58.487
1.00
35.02
L


ATOM
887
CB
ILE
117
170.299
41.393
57.902
1.00
20.96
L


ATOM
888
CG2
ILE
117
171.426
41.040
58.848
1.00
28.48
L


ATOM
889
CG1
ILE
117
170.529
40.742
56.537
1.00
16.89
L


ATOM
890
CD1
ILE
117
171.461
41.625
55.632
1.00
18.50
L


ATOM
891
C
ILE
117
169.039
39.484
58.952
1.00
32.15
L


ATOM
892
O
ILE
117
169.467
39.212
60.076
1.00
40.81
L


ATOM
893
N
PHE
118
168.626
38.560
58.091
1.00
28.82
L


ATOM
894
CA
PHE
118
168.671
37.145
58.423
1.00
22.76
L


ATOM
895
CB
PHE
118
167.260
36.601
58.654
1.00
32.60
L


ATOM
896
CG
PHE
118
166.589
37.174
59.858
1.00
32.33
L


ATOM
897
CD1
PHE
118
165.845
38.339
59.761
1.00
32.14
L


ATOM
898
CD2
PHE
118
166.731
36.572
61.100
1.00
30.97
L


ATOM
899
CE1
PHE
118
165.262
38.902
60.884
1.00
43.45
L


ATOM
900
CE2
PHE
118
166.148
37.128
62.230
1.00
24.60
L


ATOM
901
CZ
PHE
118
165.411
38.294
62.124
1.00
41.49
L


ATOM
902
C
PHE
118
169.339
36.332
57.329
1.00
26.66
L


ATOM
903
O
PHE
118
168.928
36.370
56.170
1.00
29.78
L


ATOM
904
N
PRO
119
170.402
35.602
57.685
1.00
34.94
L


ATOM
905
CD
PRO
119
170.999
35.559
59.027
1.00
32.83
L


ATOM
906
CA
PRO
119
171.136
34.763
56.734
1.00
37.60
L


ATOM
907
CB
PRO
119
172.400
34.369
57.497
1.00
31.28
L


ATOM
908
CG
PRO
119
172.428
35.261
58.732
1.00
32.18
L


ATOM
909
C
PRO
119
170.278
33.551
56.389
1.00
36.29
L


ATOM
910
O
PRO
119
169.297
33.266
57.079
1.00
43.06
L


ATOM
911
N
PRO
120
170.628
32.822
55.321
1.00
33.16
L


ATOM
912
CD
PRO
120
171.752
33.046
54.401
1.00
35.66
L


ATOM
913
CA
PRO
120
169.838
31.645
54.944
1.00
27.25
L


ATOM
914
CB
PRO
120
170.543
31.110
53.699
1.00
23.84
L


ATOM
915
CG
PRO
120
171.345
32.258
53.186
1.00
47.85
L


ATOM
916
C
PRO
120
169.771
30.597
56.052
1.00
47.51
L


ATOM
917
O
PRO
120
170.408
30.733
57.097
1.00
61.24
L


ATOM
918
N
SER
121
168.990
29.552
55.812
1.00
46.49
L


ATOM
919
CA
SER
121
168.836
28.481
56.781
1.00
35.79
L


ATOM
920
CB
SER
121
167.366
28.091
56.908
1.00
39.97
L


ATOM
921
OG
SER
121
166.848
28.512
58.153
1.00
61.95
L


ATOM
922
C
SER
121
169.643
27.264
56.366
1.00
35.14
L


ATOM
923
O
SER
121
169.776
26.970
55.178
1.00
54.90
L


ATOM
924
N
SER
122
170.190
26.566
57.352
1.00
34.00
L


ATOM
925
CA
SER
122
170.964
25.367
57.078
1.00
43.67
L


ATOM
926
CB
SER
122
171.319
24.661
58.385
1.00
55.34
L


ATOM
927
OG
SER
122
170.845
25.401
59.496
1.00
66.58
L


ATOM
928
C
SER
122
170.038
24.496
56.265
1.00
39.48
L


ATOM
929
O
SER
122
170.345
24.107
55.139
1.00
38.43
L


ATOM
930
N
GLU
123
168.878
24.223
56.850
1.00
39.62
L


ATOM
931
CA
GLU
123
167.865
23.412
56.208
1.00
42.31
L


ATOM
932
CB
GLU
123
166.569
23.487
57.012
1.00
49.13
L


ATOM
933
CG
GLU
123
165.364
22.895
56.294
1.00
85.24
L


ATOM
934
CD
GLU
123
164.237
22.520
57.240
1.00
100.00
L


ATOM
935
OE1
GLU
123
164.458
22.525
58.473
1.00
99.99
L


ATOM
936
OE2
GLU
123
163.127
22.221
56.742
1.00
99.98
L


ATOM
937
C
GLU
123
167.627
23.885
54.774
1.00
34.58
L


ATOM
938
O
GLU
123
167.599
23.079
53.843
1.00
33.93
L


ATOM
939
N
GLN
124
167.467
25.193
54.588
1.00
36.30
L


ATOM
940
CA
GLN
124
167.228
25.719
53.254
1.00
21.55
L


ATOM
941
CB
GLN
124
166.875
27.207
53.289
1.00
32.12
L


ATOM
942
CG
GLN
124
167.075
27.854
51.927
1.00
35.77
L


ATOM
943
CD
GLN
124
166.351
29.160
51.766
1.00
35.92
L


ATOM
944
OE1
GLN
124
166.349
29.995
52.669
1.00
37.01
L


ATOM
945
NE2
GLN
124
165.739
29.355
50.603
1.00
40.10
L


ATOM
946
C
GLN
124
168.441
25.534
52.353
1.00
29.80
L


ATOM
947
O
GLN
124
168.308
25.269
51.159
1.00
40.34
L


ATOM
948
N
LEU
125
169.626
25.691
52.921
1.00
29.81
L


ATOM
949
CA
LEU
125
170.837
25.538
52.142
1.00
30.50
L


ATOM
950
CB
LEU
125
172.047
25.907
52.998
1.00
28.95
L


ATOM
951
CG
LEU
125
172.296
27.418
53.004
1.00
38.05
L


ATOM
952
CD1
LEU
125
173.275
27.794
54.099
1.00
45.58
L


ATOM
953
CD2
LEU
125
172.814
27.836
51.636
1.00
18.04
L


ATOM
954
C
LEU
125
170.952
24.108
51.630
1.00
31.63
L


ATOM
955
O
LEU
125
171.396
23.873
50.503
1.00
28.04
L


ATOM
956
N
THR
126
170.530
23.156
52.454
1.00
34.33
L


ATOM
957
CA
THR
126
170.570
21.741
52.094
1.00
40.34
L


ATOM
958
CB
THR
126
169.981
20.876
53.226
1.00
47.29
L


ATOM
959
OG1
THR
126
170.445
21.364
54.490
1.00
38.65
L


ATOM
960
CG2
THR
126
170.397
19.426
53.063
1.00
58.05
L


ATOM
961
C
THR
126
169.806
21.444
50.796
1.00
47.30
L


ATOM
962
O
THR
126
170.142
20.515
50.063
1.00
54.95
L


ATOM
963
N
SER
127
168.779
22.236
50.513
1.00
57.73
L


ATOM
964
CA
SER
127
167.985
22.042
49.308
1.00
63.60
L


ATOM
965
CB
SER
127
166.551
22.525
49.542
1.00
63.78
L


ATOM
966
OG
SER
127
166.528
23.787
50.186
1.00
64.66
L


ATOM
967
C
SER
127
168.581
22.760
48.096
1.00
67.39
L


ATOM
968
O
SER
127
168.031
22.693
46.994
1.00
73.65
L


ATOM
969
N
GLY
128
169.701
23.446
48.303
1.00
60.63
L


ATOM
970
CA
GLY
128
170.350
24.154
47.209
1.00
50.24
L


ATOM
971
C
GLY
128
169.838
25.563
46.947
1.00
51.09
L


ATOM
972
O
GLY
128
170.151
26.169
45.917
1.00
49.56
L


ATOM
973
N
GLY
129
169.043
26.083
47.877
1.00
48.06
L


ATOM
974
CA
GLY
129
168.507
27.425
47.731
1.00
48.39
L


ATOM
975
C
GLY
129
168.926
28.261
48.919
1.00
45.57
L


ATOM
976
O
GLY
129
169.221
27.719
49.986
1.00
58.83
L


ATOM
977
N
ALA
130
168.964
29.577
48.746
1.00
44.51
L


ATOM
978
CA
ALA
130
169.368
30.467
49.834
1.00
42.67
L


ATOM
979
CB
ALA
130
170.868
30.721
49.766
1.00
41.38
L


ATOM
980
C
ALA
130
168.612
31.784
49.784
1.00
38.53
L


ATOM
981
O
ALA
130
168.661
32.506
48.783
1.00
43.66
L


ATOM
982
N
SER
131
167.923
32.103
50.872
1.00
34.81
L


ATOM
983
CA
SER
131
167.154
33.334
50.936
1.00
28.61
L


ATOM
984
CB
SER
131
165.662
33.019
51.035
1.00
29.28
L


ATOM
985
OG
SER
131
165.165
32.494
49.817
1.00
65.36
L


ATOM
986
C
SER
131
167.568
34.177
52.118
1.00
17.56
L


ATOM
987
O
SER
131
167.547
33.714
53.259
1.00
23.33
L


ATOM
988
N
VAL
132
167.958
35.413
51.842
1.00
21.04
L


ATOM
989
CA
VAL
132
168.353
36.345
52.891
1.00
22.72
L


ATOM
990
CB
VAL
132
169.558
37.211
52.474
1.00
24.09
L


ATOM
991
CG1
VAL
132
170.321
37.669
53.711
1.00
37.12
L


ATOM
992
CG2
VAL
132
170.468
36.420
51.535
1.00
26.09
L


ATOM
993
C
VAL
132
167.143
37.238
53.115
1.00
27.45
L


ATOM
994
O
VAL
132
166.609
37.836
52.179
1.00
34.77
L


ATOM
995
N
VAL
133
166.701
37.314
54.360
1.00
21.35
L


ATOM
996
CA
VAL
133
165.532
38.101
54.688
1.00
15.87
L


ATOM
997
CB
VAL
133
164.483
37.235
55.426
1.00
18.41
L


ATOM
998
CG1
VAL
133
163.382
38.111
56.010
1.00
41.56
L


ATOM
999
CG2
VAL
133
163.900
36.211
54.466
1.00
38.08
L


ATOM
1000
C
VAL
133
165.870
39.296
55.548
1.00
10.26
L


ATOM
1001
O
VAL
133
166.701
39.222
56.454
1.00
24.39
L


ATOM
1002
N
CYS
134
165.206
40.404
55.254
1.00
24.79
L


ATOM
1003
CA
CYS
134
165.412
41.626
55.999
1.00
33.03
L


ATOM
1004
C
CYS
134
164.070
42.163
56.444
1.00
34.77
L


ATOM
1005
O
CYS
134
163.166
42.338
55.631
1.00
37.02
L


ATOM
1006
CB
CYS
134
166.104
42.660
55.127
1.00
37.32
L


ATOM
1007
SG
CYS
134
166.705
44.083
56.077
1.00
64.48
L


ATOM
1008
N
PHE
135
163.946
42.420
57.737
1.00
28.41
L


ATOM
1009
CA
PHE
135
162.710
42.949
58.296
1.00
35.98
L


ATOM
1010
CB
PHE
135
162.297
42.152
59.536
1.00
23.45
L


ATOM
1011
CG
PHE
135
161.854
40.746
59.244
1.00
41.99
L


ATOM
1012
CD1
PHE
135
160.991
40.472
58.187
1.00
58.79
L


ATOM
1013
CD2
PHE
135
162.280
39.696
60.049
1.00
38.90
L


ATOM
1014
CE1
PHE
135
160.555
39.170
57.939
1.00
56.32
L


ATOM
1015
CE2
PHE
135
161.849
38.391
59.810
1.00
57.18
L


ATOM
1016
CZ
PHE
135
160.987
38.127
58.753
1.00
46.58
L


ATOM
1017
C
PHE
135
162.880
44.412
58.696
1.00
37.21
L


ATOM
1018
O
PHE
135
163.841
44.773
59.373
1.00
31.75
L


ATOM
1019
N
LEU
136
161.951
45.253
58.264
1.00
38.27
L


ATOM
1020
CA
LEU
136
161.968
46.665
58.622
1.00
33.10
L


ATOM
1021
CB
LEU
136
162.049
47.531
57.369
1.00
23.62
L


ATOM
1022
CG
LEU
136
163.303
47.259
56.534
1.00
17.58
L


ATOM
1023
CD1
LEU
136
163.055
46.103
55.572
1.00
17.79
L


ATOM
1024
CD2
LEU
136
163.686
48.512
55.770
1.00
29.81
L


ATOM
1025
C
LEU
136
160.632
46.839
59.319
1.00
30.65
L


ATOM
1026
O
LEU
136
159.600
47.002
58.673
1.00
30.43
L


ATOM
1027
N
ASN
137
160.651
46.779
60.643
1.00
35.92
L


ATOM
1028
CA
ASN
137
159.421
46.873
61.400
1.00
43.25
L


ATOM
1029
CB
ASN
137
159.387
45.751
62.433
1.00
42.56
L


ATOM
1030
CG
ASN
137
159.308
44.384
61.793
1.00
30.61
L


ATOM
1031
OD1
ASN
137
159.471
43.356
62.454
1.00
37.72
L


ATOM
1032
ND2
ASN
137
159.057
44.363
60.490
1.00
39.03
L


ATOM
1033
C
ASN
137
159.101
48.199
62.075
1.00
40.01
L


ATOM
1034
O
ASN
137
159.975
49.028
62.305
1.00
39.51
L


ATOM
1035
N
ASN
138
157.813
48.362
62.370
1.00
41.07
L


ATOM
1036
CA
ASN
138
157.239
49.526
63.036
1.00
38.43
L


ATOM
1037
CB
ASN
138
157.227
49.273
64.540
1.00
34.91
L


ATOM
1038
CG
ASN
138
156.667
47.916
64.883
1.00
33.75
L


ATOM
1039
OD1
ASN
138
155.592
47.806
65.459
1.00
29.26
L


ATOM
1040
ND2
ASN
138
157.402
46.864
64.537
1.00
33.12
L


ATOM
1041
C
ASN
138
157.838
50.898
62.749
1.00
31.73
L


ATOM
1042
O
ASN
138
158.582
51.447
63.559
1.00
39.37
L


ATOM
1043
N
PHE
139
157.492
51.458
61.599
1.00
32.60
L


ATOM
1044
CA
PHE
139
157.982
52.770
61.227
1.00
34.36
L


ATOM
1045
CB
PHE
139
159.138
52.644
60.237
1.00
37.95
L


ATOM
1046
CG
PHE
139
158.770
51.972
58.946
1.00
21.99
L


ATOM
1047
CD1
PHE
139
158.295
52.716
57.869
1.00
28.23
L


ATOM
1048
CD2
PHE
139
158.941
50.597
58.792
1.00
23.34
L


ATOM
1049
CE1
PHE
139
157.998
52.102
56.651
1.00
22.39
L


ATOM
1050
CE2
PHE
139
158.646
49.969
57.578
1.00
15.56
L


ATOM
1051
CZ
PHE
139
158.175
50.723
56.505
1.00
21.70
L


ATOM
1052
C
PHE
139
156.868
53.626
60.627
1.00
42.27
L


ATOM
1053
O
PHE
139
155.772
53.142
60.350
1.00
50.50
L


ATOM
1054
N
TYR
140
157.158
54.906
60.444
1.00
53.69
L


ATOM
1055
CA
TYR
140
156.205
55.847
59.883
1.00
47.40
L


ATOM
1056
CB
TYR
140
155.193
56.266
60.942
1.00
53.56
L


ATOM
1057
CG
TYR
140
154.002
56.979
60.360
1.00
71.75
L


ATOM
1058
CD1
TYR
140
154.072
58.331
60.029
1.00
76.99
L


ATOM
1059
CE1
TYR
140
152.978
58.991
59.471
1.00
91.66
L


ATOM
1060
CD2
TYR
140
152.809
56.301
60.119
1.00
85.47
L


ATOM
1061
CE2
TYR
140
151.712
56.948
59.562
1.00
96.08
L


ATOM
1062
CZ
TYR
140
151.803
58.293
59.242
1.00
93.77
L


ATOM
1063
OH
TYR
140
150.716
58.946
58.706
1.00
99.99
L


ATOM
1064
C
TYR
140
156.981
57.065
59.410
1.00
31.82
L


ATOM
1065
O
TYR
140
157.900
57.515
60.088
1.00
34.79
L


ATOM
1066
N
PRO
141
156.631
57.613
58.240
1.00
33.26
L


ATOM
1067
CD
PRO
141
157.366
58.764
57.691
1.00
26.57
L


ATOM
1068
CA
PRO
141
155.566
57.178
57.336
1.00
39.66
L


ATOM
1069
CB
PRO
141
155.450
58.321
56.323
1.00
45.22
L


ATOM
1070
CG
PRO
141
156.359
59.402
56.804
1.00
34.28
L


ATOM
1071
C
PRO
141
155.882
55.860
56.651
1.00
32.49
L


ATOM
1072
O
PRO
141
156.913
55.244
56.913
1.00
34.82
L


ATOM
1073
N
LYS
142
154.986
55.438
55.762
1.00
33.41
L


ATOM
1074
CA
LYS
142
155.157
54.191
55.033
1.00
45.13
L


ATOM
1075
CB
LYS
142
153.862
53.816
54.304
1.00
63.72
L


ATOM
1076
CG
LYS
142
152.851
54.942
54.200
1.00
82.02
L


ATOM
1077
CD
LYS
142
152.005
54.806
52.943
1.00
86.08
L


ATOM
1078
CE
LYS
142
152.031
56.087
52.114
1.00
97.81
L


ATOM
1079
NZ
LYS
142
153.221
56.173
51.209
1.00
100.00
L


ATOM
1080
C
LYS
142
156.290
54.308
54.029
1.00
47.94
L


ATOM
1081
O
LYS
142
156.926
53.320
53.679
1.00
60.89
L


ATOM
1082
N
ASP
143
156.543
55.523
53.565
1.00
61.49
L


ATOM
1083
CA
ASP
143
157.605
55.725
52.603
1.00
70.17
L


ATOM
1084
CB
ASP
143
157.730
57.203
52.259
1.00
86.10
L


ATOM
1085
CG
ASP
143
156.674
57.656
51.286
1.00
99.99
L


ATOM
1086
OD1
ASP
143
155.526
57.891
51.727
1.00
98.78
L


ATOM
1087
OD2
ASP
143
156.992
57.769
50.080
1.00
99.96
L


ATOM
1088
C
ASP
143
158.923
55.207
53.148
1.00
62.40
L


ATOM
1089
O
ASP
143
159.344
55.564
54.251
1.00
47.98
L


ATOM
1090
N
ILE
144
159.563
54.348
52.363
1.00
59.85
L


ATOM
1091
CA
ILE
144
160.839
53.752
52.722
1.00
51.41
L


ATOM
1092
CB
ILE
144
160.664
52.644
53.785
1.00
41.53
L


ATOM
1093
CG2
ILE
144
160.074
51.392
53.155
1.00
34.89
L


ATOM
1094
CG1
ILE
144
162.011
52.320
54.427
1.00
24.47
L


ATOM
1095
CD1
ILE
144
161.892
51.470
55.671
1.00
21.68
L


ATOM
1096
C
ILE
144
161.441
53.160
51.460
1.00
48.41
L


ATOM
1097
O
ILE
144
160.719
52.739
50.557
1.00
31.96
L


ATOM
1098
N
ASN
145
162.765
53.142
51.387
1.00
56.85
L


ATOM
1099
CA
ASN
145
163.447
52.603
50.221
1.00
62.49
L


ATOM
1100
CB
ASN
145
164.063
53.730
49.399
1.00
75.48
L


ATOM
1101
CG
ASN
145
164.104
53.413
47.922
1.00
98.08
L


ATOM
1102
OD1
ASN
145
163.684
54.221
47.094
1.00
99.97
L


ATOM
1103
ND2
ASN
145
164.612
52.231
47.579
1.00
99.99
L


ATOM
1104
C
ASN
145
164.531
51.633
50.650
1.00
52.75
L


ATOM
1105
O
ASN
145
165.530
52.024
51.251
1.00
46.53
L


ATOM
1106
N
VAL
146
164.324
50.362
50.330
1.00
60.86
L


ATOM
1107
CA
VAL
146
165.283
49.327
50.683
1.00
55.40
L


ATOM
1108
CB
VAL
146
164.572
48.048
51.171
1.00
51.60
L


ATOM
1109
CG1
VAL
146
165.593
47.066
51.728
1.00
23.45
L


ATOM
1110
CG2
VAL
146
163.545
48.397
52.234
1.00
57.40
L


ATOM
1111
C
VAL
146
166.158
48.975
49.490
1.00
53.23
L


ATOM
1112
O
VAL
146
165.707
48.977
48.341
1.00
69.79
L


ATOM
1113
N
LYS
147
167.421
48.679
49.775
1.00
49.86
L


ATOM
1114
CA
LYS
147
168.378
48.312
48.746
1.00
42.42
L


ATOM
1115
CB
LYS
147
169.281
49.499
48.413
1.00
47.62
L


ATOM
1116
CG
LYS
147
168.754
50.388
47.302
1.00
79.82
L


ATOM
1117
CD
LYS
147
169.895
51.010
46.512
1.00
86.84
L


ATOM
1118
CE
LYS
147
169.939
52.524
46.691
1.00
93.63
L


ATOM
1119
NZ
LYS
147
170.983
52.943
47.671
1.00
75.73
L


ATOM
1120
C
LYS
147
169.222
47.159
49.265
1.00
35.56
L


ATOM
1121
O
LYS
147
169.545
47.103
50.451
1.00
22.60
L


ATOM
1122
N
TRP
148
169.566
46.236
48.375
1.00
43.57
L


ATOM
1123
CA
TRP
148
170.386
45.095
48.758
1.00
34.06
L


ATOM
1124
CB
TRP
148
169.782
43.796
48.214
1.00
32.94
L


ATOM
1125
CG
TRP
148
168.782
43.185
49.150
1.00
47.82
L


ATOM
1126
CD2
TRP
148
169.062
42.433
50.338
1.00
46.07
L


ATOM
1127
CE2
TRP
148
167.822
42.096
50.923
1.00
40.42
L


ATOM
1128
CE3
TRP
148
170.243
42.012
50.967
1.00
64.21
L


ATOM
1129
CD1
TRP
148
167.420
43.270
49.065
1.00
34.71
L


ATOM
1130
NE1
TRP
148
166.838
42.621
50.125
1.00
38.06
L


ATOM
1131
CZ2
TRP
148
167.725
41.357
52.109
1.00
15.75
L


ATOM
1132
CZ3
TRP
148
170.146
41.277
52.148
1.00
72.60
L


ATOM
1133
CH2
TRP
148
168.894
40.958
52.705
1.00
49.76
L


ATOM
1134
C
TRP
148
171.793
45.269
48.217
1.00
36.36
L


ATOM
1135
O
TRP
148
171.984
45.624
47.055
1.00
34.43
L


ATOM
1136
N
LYS
149
172.784
45.027
49.062
1.00
44.04
L


ATOM
1137
CA
LYS
149
174.157
45.161
48.623
1.00
41.49
L


ATOM
1138
CB
LYS
149
174.820
46.341
49.321
1.00
34.89
L


ATOM
1139
CG
LYS
149
175.080
47.515
48.400
1.00
38.34
L


ATOM
1140
CD
LYS
149
174.417
48.774
48.915
1.00
36.55
L


ATOM
1141
CE
LYS
149
175.294
49.986
48.667
1.00
43.94
L


ATOM
1142
NZ
LYS
149
175.270
50.930
49.819
1.00
55.84
L


ATOM
1143
C
LYS
149
174.937
43.893
48.896
1.00
38.62
L


ATOM
1144
O
LYS
149
175.125
43.505
50.045
1.00
42.16
L


ATOM
1145
N
ILE
150
175.372
43.241
47.825
1.00
22.84
L


ATOM
1146
CA
ILE
150
176.157
42.020
47.938
1.00
24.90
L


ATOM
1147
CB
ILE
150
175.675
40.954
46.958
1.00
42.14
L


ATOM
1148
CG2
ILE
150
176.371
39.642
47.255
1.00
38.41
L


ATOM
1149
CG1
ILE
150
174.159
40.812
47.060
1.00
42.99
L


ATOM
1150
CD1
ILE
150
173.598
39.635
46.309
1.00
32.26
L


ATOM
1151
C
ILE
150
177.600
42.361
47.614
1.00
23.92
L


ATOM
1152
O
ILE
150
177.975
42.479
46.443
1.00
24.95
L


ATOM
1153
N
ASP
151
178.404
42.527
48.658
1.00
48.84
L


ATOM
1154
CA
ASP
151
179.805
42.886
48.488
1.00
63.98
L


ATOM
1155
CB
ASP
151
180.507
41.896
47.547
1.00
74.75
L


ATOM
1156
CG
ASP
151
180.844
40.574
48.223
1.00
76.70
L


ATOM
1157
OD1
ASP
151
180.681
40.465
49.460
1.00
79.84
L


ATOM
1158
OD2
ASP
151
181.274
39.638
47.508
1.00
56.23
L


ATOM
1159
C
ASP
151
179.854
44.300
47.905
1.00
70.87
L


ATOM
1160
O
ASP
151
180.483
44.536
46.868
1.00
70.42
L


ATOM
1161
N
GLY
152
179.172
45.226
48.577
1.00
76.86
L


ATOM
1162
CA
GLY
152
179.139
46.613
48.139
1.00
67.78
L


ATOM
1163
C
GLY
152
178.418
46.840
46.824
1.00
70.46
L


ATOM
1164
O
GLY
152
178.156
47.980
46.438
1.00
79.32
L


ATOM
1165
N
SER
153
178.098
45.754
46.131
1.00
62.96
L


ATOM
1166
CA
SER
153
177.409
45.845
44.856
1.00
60.58
L


ATOM
1167
CB
SER
153
177.840
44.693
43.947
1.00
68.60
L


ATOM
1168
OG
SER
153
178.171
45.160
42.653
1.00
71.40
L


ATOM
1169
C
SER
153
175.902
45.800
45.063
1.00
61.38
L


ATOM
1170
O
SER
153
175.401
45.044
45.895
1.00
59.28
L


ATOM
1171
N
GLU
154
175.183
46.620
44.304
1.00
59.43
L


ATOM
1172
CA
GLU
154
173.731
46.677
44.393
1.00
66.09
L


ATOM
1173
CB
GLU
154
173.222
47.987
43.793
1.00
81.88
L


ATOM
1174
CG
GLU
154
172.299
48.776
44.700
1.00
78.09
L


ATOM
1175
CD
GLU
154
171.857
50.087
44.079
1.00
85.76
L


ATOM
1176
OE1
GLU
154
170.891
50.074
43.283
1.00
65.69
L


ATOM
1177
OE2
GLU
154
172.476
51.131
44.385
1.00
98.75
L


ATOM
1178
C
GLU
154
173.123
45.505
43.635
1.00
78.80
L


ATOM
1179
O
GLU
154
173.476
45.248
42.485
1.00
87.43
L


ATOM
1180
N
ARG
155
172.211
44.791
44.288
1.00
78.61
L


ATOM
1181
CA
ARG
155
171.552
43.654
43.669
1.00
72.18
L


ATOM
1182
CB
ARG
155
171.904
42.371
44.411
1.00
57.25
L


ATOM
1183
CG
ARG
155
172.051
41.177
43.500
1.00
53.97
L


ATOM
1184
CD
ARG
155
170.868
40.250
43.648
1.00
47.95
L


ATOM
1185
NE
ARG
155
170.680
39.401
42.474
1.00
59.26
L


ATOM
1186
CZ
ARG
155
171.533
38.459
42.084
1.00
47.18
L


ATOM
1187
NH1
ARG
155
172.644
38.239
42.776
1.00
58.65
L


ATOM
1188
NH2
ARG
155
171.272
37.731
41.007
1.00
62.18
L


ATOM
1189
C
ARG
155
170.049
43.862
43.692
1.00
76.06
L


ATOM
1190
O
ARG
155
169.470
44.142
44.741
1.00
79.59
L


ATOM
1191
N
GLN
156
169.426
43.726
42.527
1.00
81.46
L


ATOM
1192
CA
GLN
156
167.984
43.913
42.395
1.00
75.51
L


ATOM
1193
CB
GLN
156
167.702
45.122
41.510
1.00
76.21
L


ATOM
1194
CG
GLN
156
168.779
46.179
41.579
1.00
86.59
L


ATOM
1195
CD
GLN
156
168.216
47.575
41.514
1.00
82.98
L


ATOM
1196
OE1
GLN
156
167.460
47.912
40.597
1.00
74.92
L


ATOM
1197
NE2
GLN
156
168.579
48.402
42.486
1.00
79.96
L


ATOM
1198
C
GLN
156
167.308
42.690
41.799
1.00
75.18
L


ATOM
1199
O
GLN
156
166.094
42.663
41.629
1.00
78.45
L


ATOM
1200
N
ASN
157
168.106
41.681
41.485
1.00
69.92
L


ATOM
1201
CA
ASN
157
167.577
40.460
40.909
1.00
62.62
L


ATOM
1202
CB
ASN
157
168.587
39.880
39.912
1.00
83.40
L


ATOM
1203
CG
ASN
157
168.058
38.656
39.189
1.00
96.73
L


ATOM
1204
OD1
ASN
157
168.142
37.534
39.692
1.00
99.98
L


ATOM
1205
ND2
ASN
157
167.509
38.865
38.000
1.00
96.79
L


ATOM
1206
C
ASN
157
167.270
39.440
42.009
1.00
43.07
L


ATOM
1207
O
ASN
157
168.169
38.978
42.711
1.00
51.60
L


ATOM
1208
N
GLY
158
165.994
39.103
42.166
1.00
37.93
L


ATOM
1209
CA
GLY
158
165.613
38.126
43.168
1.00
45.50
L


ATOM
1210
C
GLY
158
165.052
38.719
44.441
1.00
46.88
L


ATOM
1211
O
GLY
158
164.905
38.016
45.438
1.00
46.67
L


ATOM
1212
N
VAL
159
164.730
40.005
44.409
1.00
46.40
L


ATOM
1213
CA
VAL
159
164.191
40.668
45.582
1.00
48.04
L


ATOM
1214
CB
VAL
159
164.746
42.089
45.707
1.00
46.84
L


ATOM
1215
CG1
VAL
159
164.497
42.616
47.106
1.00
49.41
L


ATOM
1216
CG2
VAL
159
166.234
42.091
45.398
1.00
31.32
L


ATOM
1217
C
VAL
159
162.668
40.738
45.570
1.00
50.14
L


ATOM
1218
O
VAL
159
162.065
41.111
44.563
1.00
70.43
L


ATOM
1219
N
LEU
160
162.060
40.371
46.699
1.00
47.07
L


ATOM
1220
CA
LEU
160
160.606
40.379
46.862
1.00
40.66
L


ATOM
1221
CB
LEU
160
160.060
38.958
46.894
1.00
28.04
L


ATOM
1222
CG
LEU
160
160.148
38.197
45.577
1.00
32.43
L


ATOM
1223
CD1
LEU
160
159.270
36.965
45.669
1.00
37.34
L


ATOM
1224
CD2
LEU
160
159.722
39.095
44.413
1.00
7.94
L


ATOM
1225
C
LEU
160
160.204
41.077
48.151
1.00
42.42
L


ATOM
1226
O
LEU
160
160.474
40.583
49.250
1.00
48.94
L


ATOM
1227
N
ASN
161
159.545
42.220
48.011
1.00
40.58
L


ATOM
1228
CA
ASN
161
159.109
42.996
49.161
1.00
26.69
L


ATOM
1229
CB
ASN
161
159.377
44.477
48.917
1.00
24.90
L


ATOM
1230
CG
ASN
161
160.804
44.746
48.500
1.00
36.23
L


ATOM
1231
OD1
ASN
161
161.743
44.301
49.153
1.00
55.57
L


ATOM
1232
ND2
ASN
161
160.975
45.475
47.407
1.00
47.35
L


ATOM
1233
C
ASN
161
157.637
42.792
49.471
1.00
32.25
L


ATOM
1234
O
ASN
161
156.850
42.415
48.605
1.00
34.73
L


ATOM
1235
N
SER
162
157.276
43.052
50.722
1.00
28.82
L


ATOM
1236
CA
SER
162
155.908
42.907
51.179
1.00
30.10
L


ATOM
1237
CB
SER
162
155.655
41.470
51.623
1.00
25.07
L


ATOM
1238
OG
SER
162
154.368
41.341
52.203
1.00
34.98
L


ATOM
1239
C
SER
162
155.687
43.855
52.349
1.00
28.22
L


ATOM
1240
O
SER
162
156.542
43.949
53.231
1.00
28.01
L


ATOM
1241
N
TRP
163
154.556
44.560
52.346
1.00
44.68
L


ATOM
1242
CA
TRP
163
154.217
45.502
53.413
1.00
47.01
L


ATOM
1243
CB
TRP
163
153.977
46.914
52.865
1.00
47.04
L


ATOM
1244
CG
TRP
163
155.114
47.542
52.139
1.00
50.28
L


ATOM
1245
CD2
TRP
163
155.581
47.209
50.824
1.00
47.77
L


ATOM
1246
CE2
TRP
163
156.655
48.079
50.529
1.00
47.30
L


ATOM
1247
CE3
TRP
163
155.193
46.258
49.865
1.00
36.64
L


ATOM
1248
CD1
TRP
163
155.899
48.573
52.578
1.00
61.33
L


ATOM
1249
NE1
TRP
163
156.828
48.901
51.615
1.00
51.73
L


ATOM
1250
CZ2
TRP
163
157.349
48.028
49.313
1.00
41.27
L


ATOM
1251
CZ3
TRP
163
155.882
46.206
48.655
1.00
43.64
L


ATOM
1252
CH2
TRP
163
156.950
47.089
48.391
1.00
31.42
L


ATOM
1253
C
TRP
163
152.937
45.087
54.111
1.00
47.66
L


ATOM
1254
O
TRP
163
152.056
44.487
53.504
1.00
46.95
L


ATOM
1255
N
THR
164
152.831
45.420
55.389
1.00
42.09
L


ATOM
1256
CA
THR
164
151.623
45.119
56.141
1.00
39.34
L


ATOM
1257
CB
THR
164
151.927
44.805
57.604
1.00
40.04
L


ATOM
1258
OG1
THR
164
152.625
45.911
58.185
1.00
45.73
L


ATOM
1259
CG2
THR
164
152.770
43.552
57.720
1.00
45.43
L


ATOM
1260
C
THR
164
150.804
46.400
56.099
1.00
33.70
L


ATOM
1261
O
THR
164
151.268
47.424
55.597
1.00
27.93
L


ATOM
1262
N
ASP
165
149.584
46.349
56.620
1.00
29.63
L


ATOM
1263
CA
ASP
165
148.739
47.533
56.649
1.00
37.94
L


ATOM
1264
CB
ASP
165
147.261
47.141
56.673
1.00
57.52
L


ATOM
1265
CG
ASP
165
146.784
46.574
55.352
1.00
78.24
L


ATOM
1266
OD1
ASP
165
147.303
47.005
54.302
1.00
92.25
L


ATOM
1267
OD2
ASP
165
145.891
45.698
55.364
1.00
85.65
L


ATOM
1268
C
ASP
165
149.078
48.282
57.923
1.00
47.66
L


ATOM
1269
O
ASP
165
149.760
47.746
58.792
1.00
50.50
L


ATOM
1270
N
GLN
166
148.609
49.516
58.030
1.00
47.56
L


ATOM
1271
CA
GLN
166
148.850
50.319
59.220
1.00
42.28
L


ATOM
1272
CB
GLN
166
147.982
51.570
59.185
1.00
44.46
L


ATOM
1273
CG
GLN
166
148.213
52.541
60.320
1.00
29.22
L


ATOM
1274
CD
GLN
166
147.967
53.965
59.885
1.00
17.51
L


ATOM
1275
OE1
GLN
166
146.909
54.283
59.343
1.00
43.27
L


ATOM
1276
NE2
GLN
166
148.949
54.830
60.103
1.00
25.31
L


ATOM
1277
C
GLN
166
148.462
49.478
60.422
1.00
38.24
L


ATOM
1278
O
GLN
166
147.361
48.934
60.465
1.00
28.20
L


ATOM
1279
N
ASP
167
149.361
49.364
61.393
1.00
47.65
L


ATOM
1280
CA
ASP
167
149.072
48.577
62.576
1.00
59.05
L


ATOM
1281
CB
ASP
167
150.200
48.691
63.591
1.00
40.82
L


ATOM
1282
CG
ASP
167
150.022
47.742
64.752
1.00
39.12
L


ATOM
1283
OD1
ASP
167
149.966
46.517
64.510
1.00
57.63
L


ATOM
1284
OD2
ASP
167
149.927
48.214
65.902
1.00
58.29
L


ATOM
1285
C
ASP
167
147.768
49.040
63.205
1.00
68.64
L


ATOM
1286
O
ASP
167
147.482
50.237
63.274
1.00
62.87
L


ATOM
1287
N
SER
168
146.978
48.079
63.658
1.00
81.22
L


ATOM
1288
CA
SER
168
145.695
48.374
64.271
1.00
77.38
L


ATOM
1289
CB
SER
168
144.865
47.095
64.370
1.00
77.42
L


ATOM
1290
OG
SER
168
145.397
46.080
63.532
1.00
89.64
L


ATOM
1291
C
SER
168
145.839
48.994
65.651
1.00
79.26
L


ATOM
1292
O
SER
168
144.922
49.650
66.139
1.00
85.04
L


ATOM
1293
N
LYS
169
146.990
48.792
66.282
1.00
71.82
L


ATOM
1294
CA
LYS
169
147.210
49.330
67.617
1.00
55.95
L


ATOM
1295
CB
LYS
169
147.785
48.247
68.534
1.00
74.95
L


ATOM
1296
CG
LYS
169
147.259
46.849
68.253
1.00
84.17
L


ATOM
1297
CD
LYS
169
148.379
45.938
67.762
1.00
83.79
L


ATOM
1298
CE
LYS
169
147.843
44.810
66.885
1.00
85.61
L


ATOM
1299
NZ
LYS
169
148.678
44.588
65.665
1.00
81.34
L


ATOM
1300
C
LYS
169
148.114
50.556
67.658
1.00
46.88
L


ATOM
1301
O
LYS
169
147.720
51.614
68.145
1.00
59.39
L


ATOM
1302
N
ASP
170
149.331
50.418
67.142
1.00
40.42
L


ATOM
1303
CA
ASP
170
150.269
51.532
67.164
1.00
32.11
L


ATOM
1304
CB
ASP
170
151.647
51.033
67.620
1.00
37.35
L


ATOM
1305
CG
ASP
170
152.505
50.537
66.479
1.00
58.55
L


ATOM
1306
OD1
ASP
170
152.200
49.471
65.909
1.00
65.81
L


ATOM
1307
OD2
ASP
170
153.500
51.221
66.162
1.00
76.15
L


ATOM
1308
C
ASP
170
150.370
52.309
65.856
1.00
34.80
L


ATOM
1309
O
ASP
170
151.246
53.159
65.693
1.00
26.65
L


ATOM
1310
N
SER
171
149.466
52.024
64.927
1.00
29.78
L


ATOM
1311
CA
SER
171
149.429
52.719
63.648
1.00
32.11
L


ATOM
1312
CB
SER
171
148.876
54.135
63.845
1.00
36.91
L


ATOM
1313
OG
SER
171
147.612
54.105
64.486
1.00
87.97
L


ATOM
1314
C
SER
171
150.750
52.809
62.894
1.00
34.11
L


ATOM
1315
O
SER
171
151.004
53.807
62.223
1.00
25.60
L


ATOM
1316
N
THR
172
151.591
51.783
62.993
1.00
30.87
L


ATOM
1317
CA
THR
172
152.866
51.788
62.271
1.00
19.30
L


ATOM
1318
CB
THR
172
154.046
51.292
63.129
1.00
41.47
L


ATOM
1319
OG1
THR
172
153.590
50.286
64.039
1.00
61.43
L


ATOM
1320
CG2
THR
172
154.686
52.449
63.876
1.00
35.62
L


ATOM
1321
C
THR
172
152.807
50.877
61.058
1.00
25.70
L


ATOM
1322
O
THR
172
151.812
50.189
60.826
1.00
23.98
L


ATOM
1323
N
TYR
173
153.892
50.871
60.295
1.00
30.31
L


ATOM
1324
CA
TYR
173
153.985
50.042
59.109
1.00
21.84
L


ATOM
1325
CB
TYR
173
154.167
50.911
57.863
1.00
39.03
L


ATOM
1326
CG
TYR
173
152.916
51.668
57.487
1.00
42.99
L


ATOM
1327
CD1
TYR
173
151.794
50.994
57.008
1.00
41.83
L


ATOM
1328
CE1
TYR
173
150.621
51.678
56.709
1.00
59.78
L


ATOM
1329
CD2
TYR
173
152.834
53.050
57.651
1.00
48.81
L


ATOM
1330
CE2
TYR
173
151.663
53.745
57.355
1.00
38.39
L


ATOM
1331
CZ
TYR
173
150.560
53.052
56.888
1.00
60.04
L


ATOM
1332
OH
TYR
173
149.392
53.723
56.607
1.00
65.26
L


ATOM
1333
C
TYR
173
155.160
49.098
59.245
1.00
30.15
L


ATOM
1334
O
TYR
173
156.089
49.350
60.009
1.00
35.37
L


ATOM
1335
N
SER
174
155.111
48.005
58.505
1.00
26.54
L


ATOM
1336
CA
SER
174
156.184
47.035
58.539
1.00
25.11
L


ATOM
1337
CB
SER
174
155.832
45.874
59.467
1.00
32.70
L


ATOM
1338
OG
SER
174
156.004
46.246
60.824
1.00
23.41
L


ATOM
1339
C
SER
174
156.394
46.518
57.136
1.00
28.29
L


ATOM
1340
O
SER
174
155.475
46.515
56.314
1.00
22.73
L


ATOM
1341
N
MET
175
157.614
46.093
56.857
1.00
29.61
L


ATOM
1342
CA
MET
175
157.925
45.571
55.552
1.00
24.86
L


ATOM
1343
CB
MET
175
158.423
46.693
54.646
1.00
16.99
L


ATOM
1344
CG
MET
175
159.379
46.232
53.575
1.00
9.33
L


ATOM
1345
SD
MET
175
159.949
47.595
52.577
1.00
36.70
L


ATOM
1346
CE
MET
175
160.270
46.761
51.036
1.00
45.25
L


ATOM
1347
C
MET
175
158.986
44.498
55.686
1.00
31.31
L


ATOM
1348
O
MET
175
159.800
44.522
56.615
1.00
32.20
L


ATOM
1349
N
SER
176
158.947
43.544
54.765
1.00
27.89
L


ATOM
1350
CA
SER
176
159.916
42.462
54.737
1.00
33.91
L


ATOM
1351
CB
SER
176
159.263
41.137
55.135
1.00
32.59
L


ATOM
1352
OG
SER
176
159.019
40.331
53.997
1.00
35.30
L


ATOM
1353
C
SER
176
160.435
42.382
53.312
1.00
22.54
L


ATOM
1354
O
SER
176
159.669
42.497
52.356
1.00
28.72
L


ATOM
1355
N
SER
177
161.743
42.210
53.169
1.00
21.03
L


ATOM
1356
CA
SER
177
162.355
42.107
51.856
1.00
27.57
L


ATOM
1357
CB
SER
177
163.231
43.320
51.574
1.00
32.40
L


ATOM
1358
OG
SER
177
163.713
43.272
50.245
1.00
27.68
L


ATOM
1359
C
SER
177
163.196
40.847
51.802
1.00
22.76
L


ATOM
1360
O
SER
177
164.047
40.621
52.661
1.00
33.72
L


ATOM
1361
N
THR
178
162.956
40.026
50.788
1.00
18.89
L


ATOM
1362
CA
THR
178
163.687
38.780
50.656
1.00
16.96
L


ATOM
1363
CB
THR
178
162.740
37.580
50.740
1.00
21.10
L


ATOM
1364
OG1
THR
178
161.938
37.681
51.922
1.00
36.63
L


ATOM
1365
CG2
THR
178
163.533
36.285
50.782
1.00
22.64
L


ATOM
1366
C
THR
178
164.481
38.675
49.362
1.00
21.04
L


ATOM
1367
O
THR
178
163.949
38.878
48.266
1.00
32.90
L


ATOM
1368
N
LEU
179
165.764
38.358
49.515
1.00
23.97
L


ATOM
1369
CA
LEU
179
166.679
38.183
48.395
1.00
27.62
L


ATOM
1370
CB
LEU
179
168.000
38.899
48.669
1.00
25.50
L


ATOM
1371
CG
LEU
179
169.043
38.856
47.557
1.00
25.95
L


ATOM
1372
CD1
LEU
179
168.487
39.491
46.291
1.00
44.04
L


ATOM
1373
CD2
LEU
179
170.289
39.591
48.025
1.00
24.41
L


ATOM
1374
C
LEU
179
166.920
36.687
48.293
1.00
22.54
L


ATOM
1375
O
LEU
179
167.417
36.070
49.236
1.00
16.26
L


ATOM
1376
N
THR
180
166.558
36.110
47.153
1.00
27.81
L


ATOM
1377
CA
THR
180
166.699
34.675
46.941
1.00
38.84
L


ATOM
1378
CB
THR
180
165.343
34.060
46.531
1.00
58.84
L


ATOM
1379
OG1
THR
180
164.391
34.253
47.585
1.00
69.01
L


ATOM
1380
CG2
THR
180
165.489
32.571
46.243
1.00
65.13
L


ATOM
1381
C
THR
180
167.744
34.304
45.893
1.00
38.32
L


ATOM
1382
O
THR
180
167.658
34.715
44.733
1.00
23.89
L


ATOM
1383
N
LEU
181
168.726
33.515
46.318
1.00
50.48
L


ATOM
1384
CA
LEU
181
169.796
33.059
45.439
1.00
54.78
L


ATOM
1385
CB
LEU
181
171.118
33.751
45.782
1.00
57.52
L


ATOM
1386
CG
LEU
181
171.097
35.239
46.126
1.00
52.16
L


ATOM
1387
CD1
LEU
181
171.777
35.466
47.468
1.00
64.66
L


ATOM
1388
CD2
LEU
181
171.800
36.017
45.032
1.00
59.17
L


ATOM
1389
C
LEU
181
169.978
31.558
45.595
1.00
45.72
L


ATOM
1390
O
LEU
181
169.250
30.904
46.347
1.00
53.82
L


ATOM
1391
N
THR
182
170.964
31.021
44.882
1.00
33.32
L


ATOM
1392
CA
THR
182
171.268
29.601
44.935
1.00
32.86
L


ATOM
1393
CB
THR
182
171.740
29.086
43.571
1.00
35.79
L


ATOM
1394
OG1
THR
182
172.994
29.694
43.241
1.00
51.80
L


ATOM
1395
CG2
THR
182
170.726
29.434
42.497
1.00
10.97
L


ATOM
1396
C
THR
182
172.371
29.340
45.955
1.00
31.89
L


ATOM
1397
O
THR
182
173.084
30.256
46.366
1.00
22.49
L


ATOM
1398
N
LYS
183
172.498
28.086
46.369
1.00
18.19
L


ATOM
1399
CA
LYS
183
173.521
27.703
47.326
1.00
22.39
L


ATOM
1400
CB
LYS
183
173.539
26.186
47.492
1.00
27.09
L


ATOM
1401
CG
LYS
183
173.959
25.715
48.868
1.00
21.06
L


ATOM
1402
CD
LYS
183
174.795
24.443
48.785
1.00
28.23
L


ATOM
1403
CE
LYS
183
175.371
24.075
50.148
1.00
65.45
L


ATOM
1404
NZ
LYS
183
176.860
24.085
50.151
1.00
66.90
L


ATOM
1405
C
LYS
183
174.847
28.168
46.750
1.00
30.33
L


ATOM
1406
O
LYS
183
175.732
28.640
47.462
1.00
47.79
L


ATOM
1407
N
ASP
184
174.957
28.044
45.436
1.00
33.23
L


ATOM
1408
CA
ASP
184
176.162
28.422
44.729
1.00
32.28
L


ATOM
1409
CB
ASP
184
176.089
27.931
43.290
1.00
41.02
L


ATOM
1410
CG
ASP
184
176.041
26.424
43.199
1.00
63.85
L


ATOM
1411
OD1
ASP
184
176.516
25.754
44.147
1.00
80.57
L


ATOM
1412
OD2
ASP
184
175.530
25.907
42.185
1.00
76.42
L


ATOM
1413
C
ASP
184
176.443
29.910
44.747
1.00
37.31
L


ATOM
1414
O
ASP
184
177.250
30.379
45.547
1.00
58.28
L


ATOM
1415
N
GLU
185
175.770
30.652
43.873
1.00
33.24
L


ATOM
1416
CA
GLU
185
175.994
32.088
43.781
1.00
36.62
L


ATOM
1417
CB
GLU
185
175.088
32.691
42.702
1.00
37.34
L


ATOM
1418
CG
GLU
185
173.899
33.484
43.198
1.00
71.02
L


ATOM
1419
CD
GLU
185
172.973
33.876
42.058
1.00
78.07
L


ATOM
1420
OE1
GLU
185
173.399
34.680
41.198
1.00
94.70
L


ATOM
1421
OE2
GLU
185
171.826
33.378
42.020
1.00
82.48
L


ATOM
1422
C
GLU
185
175.870
32.849
45.095
1.00
40.77
L


ATOM
1423
O
GLU
185
176.154
34.039
45.144
1.00
28.19
L


ATOM
1424
N
TYR
186
175.455
32.171
46.160
1.00
45.72
L


ATOM
1425
CA
TYR
186
175.357
32.824
47.461
1.00
31.13
L


ATOM
1426
CB
TYR
186
174.183
32.284
48.271
1.00
29.29
L


ATOM
1427
CG
TYR
186
174.394
32.396
49.769
1.00
30.13
L


ATOM
1428
CD1
TYR
186
174.131
33.590
50.447
1.00
33.39
L


ATOM
1429
CE1
TYR
186
174.314
33.694
51.833
1.00
36.37
L


ATOM
1430
CD2
TYR
186
174.850
31.310
50.509
1.00
34.49
L


ATOM
1431
CE2
TYR
186
175.036
31.402
51.893
1.00
18.79
L


ATOM
1432
CZ
TYR
186
174.766
32.594
52.549
1.00
34.18
L


ATOM
1433
OH
TYR
186
174.933
32.673
53.913
1.00
39.71
L


ATOM
1434
C
TYR
186
176.641
32.530
48.224
1.00
25.69
L


ATOM
1435
O
TYR
186
177.138
33.358
48.985
1.00
46.65
L


ATOM
1436
N
GLU
187
177.163
31.327
48.025
1.00
33.86
L


ATOM
1437
CA
GLU
187
178.380
30.916
48.695
1.00
30.31
L


ATOM
1438
CB
GLU
187
178.478
29.394
48.707
1.00
51.43
L


ATOM
1439
CG
GLU
187
177.688
28.757
49.831
1.00
68.32
L


ATOM
1440
CD
GLU
187
177.931
27.269
49.944
1.00
84.34
L


ATOM
1441
OE1
GLU
187
178.505
26.681
49.000
1.00
72.88
L


ATOM
1442
OE2
GLU
187
177.540
26.690
50.980
1.00
92.94
L


ATOM
1443
C
GLU
187
179.608
31.516
48.021
1.00
25.34
L


ATOM
1444
O
GLU
187
180.731
31.319
48.476
1.00
33.80
L


ATOM
1445
N
ARG
188
179.393
32.255
46.937
1.00
25.18
L


ATOM
1446
CA
ARG
188
180.504
32.887
46.238
1.00
28.26
L


ATOM
1447
CB
ARG
188
180.430
32.583
44.732
1.00
50.64
L


ATOM
1448
CG
ARG
188
179.374
33.352
43.952
1.00
42.62
L


ATOM
1449
CD
ARG
188
179.332
32.895
42.493
1.00
43.87
L


ATOM
1450
NE
ARG
188
179.213
31.440
42.378
1.00
93.56
L


ATOM
1451
CZ
ARG
188
180.106
30.659
41.779
1.00
99.99
L


ATOM
1452
NH1
ARG
188
181.192
31.187
41.230
1.00
99.99
L


ATOM
1453
NH2
ARG
188
179.918
29.345
41.733
1.00
99.98
L


ATOM
1454
C
ARG
188
180.549
34.399
46.490
1.00
22.65
L


ATOM
1455
O
ARG
188
181.077
35.160
45.679
1.00
30.16
L


ATOM
1456
N
HIS
189
180.001
34.819
47.626
1.00
27.86
L


ATOM
1457
CA
HIS
189
179.979
36.223
48.023
1.00
21.88
L


ATOM
1458
CB
HIS
189
178.650
36.871
47.650
1.00
40.07
L


ATOM
1459
CG
HIS
189
178.480
37.107
46.182
1.00
47.15
L


ATOM
1460
CD2
HIS
189
178.796
38.171
45.405
1.00
55.79
L


ATOM
1461
ND1
HIS
189
177.891
36.185
45.342
1.00
63.86
L


ATOM
1462
CE1
HIS
189
177.849
36.672
44.115
1.00
61.64
L


ATOM
1463
NE2
HIS
189
178.391
37.876
44.127
1.00
60.31
L


ATOM
1464
C
HIS
189
180.152
36.239
49.531
1.00
29.34
L


ATOM
1465
O
HIS
189
179.750
35.291
50.205
1.00
34.87
L


ATOM
1466
N
ASN
190
180.729
37.309
50.067
1.00
37.34
L


ATOM
1467
CA
ASN
190
180.961
37.379
51.507
1.00
47.66
L


ATOM
1468
CB
ASN
190
182.388
37.855
51.795
1.00
68.12
L


ATOM
1469
CG
ASN
190
183.417
37.167
50.930
1.00
100.00
L


ATOM
1470
OD1
ASN
190
183.407
35.945
50.784
1.00
99.97
L


ATOM
1471
ND2
ASN
190
184.320
37.954
50.350
1.00
99.98
L


ATOM
1472
C
ASN
190
180.001
38.252
52.290
1.00
42.97
L


ATOM
1473
O
ASN
190
179.383
37.797
53.259
1.00
33.80
L


ATOM
1474
N
SER
191
179.885
39.511
51.892
1.00
39.67
L


ATOM
1475
CA
SER
191
179.010
40.419
52.614
1.00
30.33
L


ATOM
1476
CB
SER
191
179.698
41.774
52.801
1.00
31.09
L


ATOM
1477
OG
SER
191
179.887
42.056
54.177
1.00
59.06
L


ATOM
1478
C
SER
191
177.655
40.634
51.967
1.00
34.40
L


ATOM
1479
O
SER
191
177.550
40.836
50.753
1.00
48.04
L


ATOM
1480
N
TYR
192
176.620
40.574
52.802
1.00
25.05
L


ATOM
1481
CA
TYR
192
175.246
40.785
52.361
1.00
22.04
L


ATOM
1482
CB
TYR
192
174.420
39.517
52.582
1.00
16.56
L


ATOM
1483
CG
TYR
192
174.807
38.444
51.598
1.00
33.82
L


ATOM
1484
CD1
TYR
192
174.383
38.511
50.273
1.00
39.72
L


ATOM
1485
CE1
TYR
192
174.829
37.592
49.330
1.00
33.77
L


ATOM
1486
CD2
TYR
192
175.683
37.417
51.961
1.00
36.76
L


ATOM
1487
CE2
TYR
192
176.135
36.492
51.024
1.00
36.40
L


ATOM
1488
CZ
TYR
192
175.707
36.587
49.711
1.00
39.55
L


ATOM
1489
OH
TYR
192
176.164
35.693
48.766
1.00
31.96
L


ATOM
1490
C
TYR
192
174.705
41.961
53.160
1.00
26.50
L


ATOM
1491
O
TYR
192
174.749
41.970
54.389
1.00
26.85
L


ATOM
1492
N
THR
193
174.205
42.959
52.443
1.00
42.59
L


ATOM
1493
CA
THR
193
173.716
44.165
53.086
1.00
49.57
L


ATOM
1494
CB
THR
193
174.621
45.362
52.738
1.00
59.55
L


ATOM
1495
OG1
THR
193
175.983
45.025
53.025
1.00
51.20
L


ATOM
1496
CG2
THR
193
174.219
46.592
53.536
1.00
59.94
L


ATOM
1497
C
THR
193
172.285
44.574
52.777
1.00
46.91
L


ATOM
1498
O
THR
193
171.848
44.573
51.621
1.00
57.88
L


ATOM
1499
N
CYS
194
171.578
44.946
53.836
1.00
40.06
L


ATOM
1500
CA
CYS
194
170.208
45.409
53.738
1.00
39.66
L


ATOM
1501
C
CYS
194
170.229
46.894
54.090
1.00
50.91
L


ATOM
1502
O
CYS
194
170.419
47.263
55.253
1.00
50.44
L


ATOM
1503
CB
CYS
194
169.326
44.652
54.719
1.00
56.36
L


ATOM
1504
SG
CYS
194
167.606
45.219
54.662
1.00
57.55
L


ATOM
1505
N
GLU
195
170.039
47.734
53.080
1.00
62.99
L


ATOM
1506
CA
GLU
195
170.066
49.182
53.262
1.00
56.68
L


ATOM
1507
CB
GLU
195
171.006
49.802
52.231
1.00
67.85
L


ATOM
1508
CG
GLU
195
171.618
51.121
52.650
1.00
75.89
L


ATOM
1509
CD
GLU
195
172.492
51.711
51.560
1.00
66.08
L


ATOM
1510
OE1
GLU
195
171.941
52.170
50.533
1.00
83.91
L


ATOM
1511
OE2
GLU
195
173.731
51.710
51.731
1.00
69.63
L


ATOM
1512
C
GLU
195
168.689
49.812
53.128
1.00
41.96
L


ATOM
1513
O
GLU
195
167.926
49.466
52.224
1.00
46.93
L


ATOM
1514
N
ALA
196
168.381
50.755
54.014
1.00
39.48
L


ATOM
1515
CA
ALA
196
167.085
51.419
53.983
1.00
55.44
L


ATOM
1516
CB
ALA
196
166.114
50.674
54.880
1.00
62.95
L


ATOM
1517
C
ALA
196
167.111
52.897
54.375
1.00
56.78
L


ATOM
1518
O
ALA
196
167.572
53.254
55.460
1.00
57.46
L


ATOM
1519
N
THR
197
166.606
53.748
53.484
1.00
59.26
L


ATOM
1520
CA
THR
197
166.532
55.186
53.738
1.00
71.50
L


ATOM
1521
CB
THR
197
166.971
56.023
52.502
1.00
77.13
L


ATOM
1522
OG1
THR
197
166.548
55.374
51.295
1.00
83.37
L


ATOM
1523
CG2
THR
197
168.486
56.185
52.483
1.00
62.62
L


ATOM
1524
C
THR
197
165.086
55.538
54.086
1.00
73.26
L


ATOM
1525
O
THR
197
164.155
55.182
53.362
1.00
73.27
L


ATOM
1526
N
HIS
198
164.901
56.233
55.200
1.00
66.28
L


ATOM
1527
CA
HIS
198
163.570
56.617
55.655
1.00
65.29
L


ATOM
1528
CB
HIS
198
163.203
55.777
56.889
1.00
59.65
L


ATOM
1529
CG
HIS
198
161.802
55.976
57.386
1.00
50.05
L


ATOM
1530
CD2
HIS
198
161.333
56.281
58.619
1.00
10.46
L


ATOM
1531
ND1
HIS
198
160.691
55.811
56.586
1.00
23.72
L


ATOM
1532
CE1
HIS
198
159.601
56.009
57.306
1.00
8.26
L


ATOM
1533
NE2
HIS
198
159.962
56.295
58.545
1.00
26.12
L


ATOM
1534
C
HIS
198
163.611
58.101
56.001
1.00
65.53
L


ATOM
1535
O
HIS
198
164.642
58.601
56.446
1.00
44.38
L


ATOM
1536
N
LYS
199
162.501
58.804
55.776
1.00
69.60
L


ATOM
1537
CA
LYS
199
162.407
60.235
56.078
1.00
62.83
L


ATOM
1538
CB
LYS
199
160.940
60.665
56.214
1.00
59.10
L


ATOM
1539
CG
LYS
199
160.168
60.725
54.908
1.00
66.79
L


ATOM
1540
CD
LYS
199
159.541
59.376
54.566
1.00
77.11
L


ATOM
1541
CE
LYS
199
160.458
58.543
53.670
1.00
66.29
L


ATOM
1542
NZ
LYS
199
160.372
58.922
52.226
1.00
71.28
L


ATOM
1543
C
LYS
199
163.100
60.459
57.409
1.00
54.65
L


ATOM
1544
O
LYS
199
163.853
61.418
57.596
1.00
52.78
L


ATOM
1545
N
THR
200
162.823
59.538
58.322
1.00
59.05
L


ATOM
1546
CA
THR
200
163.361
59.542
59.668
1.00
72.79
L


ATOM
1547
CB
THR
200
162.896
58.262
60.404
1.00
72.74
L


ATOM
1548
OG1
THR
200
162.485
58.594
61.732
1.00
81.55
L


ATOM
1549
CG2
THR
200
164.002
57.222
60.439
1.00
67.03
L


ATOM
1550
C
THR
200
164.890
59.648
59.712
1.00
77.51
L


ATOM
1551
O
THR
200
165.479
59.774
60.785
1.00
76.37
L


ATOM
1552
N
SER
201
165.536
59.607
58.550
1.00
82.04
L


ATOM
1553
CA
SER
201
166.991
59.696
58.521
1.00
77.00
L


ATOM
1554
CB
SER
201
167.599
58.411
59.082
1.00
70.20
L


ATOM
1555
OG
SER
201
168.828
58.678
59.728
1.00
48.40
L


ATOM
1556
C
SER
201
167.583
59.967
57.145
1.00
75.86
L


ATOM
1557
O
SER
201
167.126
59.436
56.129
1.00
78.72
L


ATOM
1558
N
THR
202
168.617
60.802
57.128
1.00
79.38
L


ATOM
1559
CA
THR
202
169.317
61.144
55.897
1.00
85.40
L


ATOM
1560
CB
THR
202
169.864
62.586
55.942
1.00
82.98
L


ATOM
1561
OG1
THR
202
170.536
62.807
57.190
1.00
58.44
L


ATOM
1562
CG2
THR
202
168.725
63.591
55.800
1.00
81.93
L


ATOM
1563
C
THR
202
170.481
60.165
55.752
1.00
87.12
L


ATOM
1564
O
THR
202
171.106
60.069
54.697
1.00
90.15
L


ATOM
1565
N
SER
203
170.757
59.442
56.835
1.00
85.15
L


ATOM
1566
CA
SER
203
171.826
58.452
56.866
1.00
85.74
L


ATOM
1567
CB
SER
203
172.589
58.538
58.194
1.00
91.67
L


ATOM
1568
OG
SER
203
172.049
59.540
59.041
1.00
86.93
L


ATOM
1569
C
SER
203
171.203
57.069
56.719
1.00
87.87
L


ATOM
1570
O
SER
203
170.682
56.508
57.686
1.00
84.65
L


ATOM
1571
N
PRO
204
171.244
56.501
55.502
1.00
89.56
L


ATOM
1572
CD
PRO
204
171.858
57.072
54.292
1.00
93.06
L


ATOM
1573
CA
PRO
204
170.672
55.176
55.244
1.00
82.77
L


ATOM
1574
CB
PRO
204
171.269
54.772
53.888
1.00
81.38
L


ATOM
1575
CG
PRO
204
172.262
55.855
53.525
1.00
78.75
L


ATOM
1576
C
PRO
204
171.002
54.167
56.335
1.00
76.12
L


ATOM
1577
O
PRO
204
172.169
53.960
56.656
1.00
79.62
L


ATOM
1578
N
ILE
205
169.971
53.557
56.911
1.00
69.79
L


ATOM
1579
CA
ILE
205
170.172
52.556
57.953
1.00
59.52
L


ATOM
1580
CB
ILE
205
168.862
52.224
58.675
1.00
59.30
L


ATOM
1581
CG2
ILE
205
169.124
51.192
59.763
1.00
62.73
L


ATOM
1582
CG1
ILE
205
168.264
53.503
59.263
1.00
51.85
L


ATOM
1583
CD1
ILE
205
166.912
53.310
59.921
1.00
48.97
L


ATOM
1584
C
ILE
205
170.711
51.294
57.294
1.00
58.53
L


ATOM
1585
O
ILE
205
170.225
50.873
56.241
1.00
60.90
L


ATOM
1586
N
VAL
206
171.719
50.691
57.918
1.00
53.53
L


ATOM
1587
CA
VAL
206
172.332
49.498
57.356
1.00
41.22
L


ATOM
1588
CB
VAL
206
173.739
49.825
56.810
1.00
18.34
L


ATOM
1589
CG1
VAL
206
174.486
48.548
56.467
1.00
13.15
L


ATOM
1590
CG2
VAL
206
173.618
50.706
55.580
1.00
13.65
L


ATOM
1591
C
VAL
206
172.442
48.336
58.331
1.00
44.96
L


ATOM
1592
O
VAL
206
172.747
48.517
59.509
1.00
44.72
L


ATOM
1593
N
LYS
207
172.180
47.137
57.818
1.00
51.44
L


ATOM
1594
CA
LYS
207
172.269
45.915
58.598
1.00
52.57
L


ATOM
1595
CB
LYS
207
170.882
45.438
59.020
1.00
54.70
L


ATOM
1596
CG
LYS
207
170.800
45.038
60.479
1.00
47.68
L


ATOM
1597
CD
LYS
207
171.141
46.206
61.394
1.00
42.68
L


ATOM
1598
CE
LYS
207
170.477
46.060
62.758
1.00
53.93
L


ATOM
1599
NZ
LYS
207
170.763
44.740
63.395
1.00
56.72
L


ATOM
1600
C
LYS
207
172.906
44.900
57.671
1.00
51.25
L


ATOM
1601
O
LYS
207
172.357
44.592
56.610
1.00
57.87
L


ATOM
1602
N
SER
208
174.072
44.396
58.059
1.00
48.56
L


ATOM
1603
CA
SER
208
174.780
43.429
57.230
1.00
44.28
L


ATOM
1604
CB
SER
208
175.956
44.095
56.525
1.00
43.06
L


ATOM
1605
OG
SER
208
175.511
45.119
55.659
1.00
64.64
L


ATOM
1606
C
SER
208
175.301
42.236
58.003
1.00
46.92
L


ATOM
1607
O
SER
208
175.121
42.129
59.221
1.00
49.18
L


ATOM
1608
N
PHE
209
175.950
41.342
57.269
1.00
47.47
L


ATOM
1609
CA
PHE
209
176.539
40.152
57.850
1.00
48.36
L


ATOM
1610
CB
PHE
209
175.443
39.170
58.296
1.00
58.47
L


ATOM
1611
CG
PHE
209
174.804
38.414
57.168
1.00
68.93
L


ATOM
1612
CD1
PHE
209
173.734
38.960
56.471
1.00
77.87
L


ATOM
1613
CD2
PHE
209
175.280
37.161
56.793
1.00
73.00
L


ATOM
1614
CE1
PHE
209
173.148
38.270
55.411
1.00
71.56
L


ATOM
1615
CE2
PHE
209
174.703
36.461
55.736
1.00
64.61
L


ATOM
1616
CZ
PHE
209
173.633
37.017
55.043
1.00
71.74
L


ATOM
1617
C
PHE
209
177.481
39.498
56.851
1.00
44.99
L


ATOM
1618
O
PHE
209
177.336
39.658
55.637
1.00
35.92
L


ATOM
1619
N
ASN
210
178.463
38.782
57.384
1.00
56.65
L


ATOM
1620
CA
ASN
210
179.444
38.079
56.572
1.00
60.29
L


ATOM
1621
CB
ASN
210
180.853
38.402
57.064
1.00
70.34
L


ATOM
1622
CG
ASN
210
181.925
37.920
56.112
1.00
100.00
L


ATOM
1623
OD1
ASN
210
182.386
38.670
55.250
1.00
99.98
L


ATOM
1624
ND2
ASN
210
182.328
36.658
56.258
1.00
100.00
L


ATOM
1625
C
ASN
210
179.165
36.592
56.741
1.00
63.50
L


ATOM
1626
O
ASN
210
178.624
36.183
57.763
1.00
79.33
L


ATOM
1627
N
ARG
211
179.524
35.780
55.756
1.00
60.96
L


ATOM
1628
CA
ARG
211
179.284
34.350
55.872
1.00
67.53
L


ATOM
1629
CB
ARG
211
179.672
33.644
54.576
1.00
53.17
L


ATOM
1630
CG
ARG
211
178.475
33.141
53.792
1.00
46.30
L


ATOM
1631
CD
ARG
211
178.602
33.466
52.320
1.00
24.02
L


ATOM
1632
NE
ARG
211
179.366
32.449
51.606
1.00
24.23
L


ATOM
1633
CZ
ARG
211
180.693
32.431
51.527
1.00
46.60
L


ATOM
1634
NH1
ARG
211
181.411
33.377
52.118
1.00
74.04
L


ATOM
1635
NH2
ARG
211
181.307
31.466
50.863
1.00
58.03
L


ATOM
1636
C
ARG
211
180.041
33.746
57.060
1.00
78.01
L


ATOM
1637
O
ARG
211
181.277
33.714
57.080
1.00
62.45
L


ATOM
1638
N
ASN
212
179.264
33.284
58.046
1.00
96.28
L


ATOM
1639
CA
ASN
212
179.747
32.661
59.288
1.00
99.99
L


ATOM
1640
CB
ASN
212
181.065
31.915
59.055
1.00
99.98
L


ATOM
1641
CG
ASN
212
181.130
30.602
59.816
1.00
100.00
L


ATOM
1642
OD1
ASN
212
180.343
30.360
60.738
1.00
94.94
L


ATOM
1643
ND2
ASN
212
182.070
29.743
59.434
1.00
99.99
L


ATOM
1644
C
ASN
212
179.918
33.640
60.455
1.00
99.98
L


ATOM
1645
O
ASN
212
180.791
34.533
60.359
1.00
99.99
L


ATOM
1646
OT
ASN
212
181.064
34.157
61.055
1.00
99.99
L


ATOM
1647
CB
VAL
2
121.621
36.267
64.620
1.00
37.38
H


ATOM
1648
CG1
VAL
2
120.401
36.241
65.515
1.00
27.71
H


ATOM
1649
CG2
VAL
2
122.281
37.627
64.680
1.00
34.18
H


ATOM
1650
C
VAL
2
123.855
35.223
64.193
1.00
42.92
H


ATOM
1651
O
VAL
2
124.332
36.300
63.840
1.00
42.57
H


ATOM
1652
N
VAL
2
122.979
35.304
66.494
1.00
47.65
H


ATOM
1653
CA
VAL
2
122.610
35.160
65.057
1.00
44.59
H


ATOM
1654
N
GLN
3
124.382
34.056
63.852
1.00
45.14
H


ATOM
1655
CA
GLN
3
125.594
33.990
63.047
1.00
47.76
H


ATOM
1656
CB
GLN
3
126.585
33.018
63.684
1.00
47.79
H


ATOM
1657
CG
GLN
3
128.013
33.216
63.226
1.00
70.50
H


ATOM
1658
CD
GLN
3
128.654
31.926
62.768
1.00
77.13
H


ATOM
1659
OE1
GLN
3
128.477
30.879
63.392
1.00
89.69
H


ATOM
1660
NE2
GLN
3
129.403
31.990
61.671
1.00
74.80
H


ATOM
1661
C
GLN
3
125.324
33.576
61.605
1.00
43.77
H


ATOM
1662
O
GLN
3
124.223
33.144
61.264
1.00
28.11
H


ATOM
1663
N
LEU
4
126.341
33.721
60.761
1.00
43.76
H


ATOM
1664
CA
LEU
4
126.231
33.358
59.356
1.00
47.78
H


ATOM
1665
CB
LEU
4
126.061
34.620
58.502
1.00
41.23
H


ATOM
1666
CG
LEU
4
125.041
34.700
57.351
1.00
30.41
H


ATOM
1667
CD1
LEU
4
124.005
33.592
57.439
1.00
25.02
H


ATOM
1668
CD2
LEU
4
124.353
36.059
57.404
1.00
18.34
H


ATOM
1669
C
LEU
4
127.499
32.620
58.945
1.00
47.69
H


ATOM
1670
O
LEU
4
128.468
33.245
58.524
1.00
52.13
H


ATOM
1671
N
GLN
5
127.500
31.296
59.081
1.00
46.16
H


ATOM
1672
CA
GLN
5
128.669
30.500
58.706
1.00
47.49
H


ATOM
1673
CB
GLN
5
128.591
29.109
59.335
1.00
47.97
H


ATOM
1674
CG
GLN
5
129.142
29.029
60.747
1.00
41.56
H


ATOM
1675
CD
GLN
5
128.328
28.100
61.633
1.00
64.06
H


ATOM
1676
OE1
GLN
5
128.475
26.874
61.573
1.00
60.56
H


ATOM
1677
NE2
GLN
5
127.459
28.681
62.459
1.00
60.41
H


ATOM
1678
C
GLN
5
128.764
30.379
57.187
1.00
36.59
H


ATOM
1679
O
GLN
5
127.760
30.146
56.519
1.00
31.08
H


ATOM
1680
N
GLN
6
129.978
30.531
56.659
1.00
35.83
H


ATOM
1681
CA
GLN
6
130.214
30.460
55.220
1.00
33.41
H


ATOM
1682
CB
GLN
6
130.697
31.824
54.715
1.00
15.61
H


ATOM
1683
CG
GLN
6
130.089
32.252
53.377
1.00
48.11
H


ATOM
1684
CD
GLN
6
130.061
33.762
53.195
1.00
44.23
H


ATOM
1685
OE1
GLN
6
129.429
34.480
53.971
1.00
44.77
H


ATOM
1686
NE2
GLN
6
130.750
34.251
52.164
1.00
43.09
H


ATOM
1687
C
GLN
6
131.230
29.393
54.818
1.00
39.29
H


ATOM
1688
O
GLN
6
131.746
28.655
55.659
1.00
47.11
H


ATOM
1689
N
SER
7
131.492
29.318
53.514
1.00
52.14
H


ATOM
1690
CA
SER
7
132.464
28.402
52.913
1.00
45.28
H


ATOM
1691
CB
SER
7
133.561
28.037
53.913
1.00
30.02
H


ATOM
1692
OG
SER
7
134.775
27.764
53.240
1.00
36.01
H


ATOM
1693
C
SER
7
131.963
27.125
52.255
1.00
53.29
H


ATOM
1694
O
SER
7
130.825
26.691
52.435
1.00
41.78
H


ATOM
1695
N
GLY
8
132.873
26.546
51.478
1.00
74.11
H


ATOM
1696
CA
GLY
8
132.656
25.313
50.744
1.00
86.06
H


ATOM
1697
C
GLY
8
133.987
25.088
50.046
1.00
96.74
H


ATOM
1698
O
GLY
8
135.032
25.207
50.691
1.00
99.99
H


ATOM
1699
N
PRO
9
133.999
24.757
48.739
1.00
99.99
H


ATOM
1700
CD
PRO
9
132.851
24.529
47.850
1.00
99.99
H


ATOM
1701
CA
PRO
9
135.283
24.547
48.047
1.00
95.17
H


ATOM
1702
CB
PRO
9
134.873
23.925
46.702
1.00
99.99
H


ATOM
1703
CG
PRO
9
133.404
23.557
46.852
1.00
99.98
H


ATOM
1704
C
PRO
9
136.019
25.882
47.872
1.00
83.28
H


ATOM
1705
O
PRO
9
135.387
26.937
47.793
1.00
73.84
H


ATOM
1706
N
GLU
10
137.349
25.847
47.811
1.00
73.05
H


ATOM
1707
CA
GLU
10
138.109
27.087
47.672
1.00
56.11
H


ATOM
1708
CB
GLU
10
139.003
27.279
48.898
1.00
43.53
H


ATOM
1709
CG
GLU
10
138.487
26.554
50.136
1.00
30.69
H


ATOM
1710
CD
GLU
10
137.988
27.497
51.217
1.00
42.43
H


ATOM
1711
OE1
GLU
10
138.782
28.352
51.678
1.00
16.81
H


ATOM
1712
OE2
GLU
10
136.803
27.374
51.612
1.00
22.46
H


ATOM
1713
C
GLU
10
138.939
27.223
46.393
1.00
47.28
H


ATOM
1714
O
GLU
10
139.302
28.336
46.017
1.00
48.73
H


ATOM
1715
N
LEU
11
139.239
26.106
45.730
1.00
37.54
H


ATOM
1716
CA
LEU
11
140.012
26.137
44.486
1.00
30.14
H


ATOM
1717
CB
LEU
11
141.398
25.517
44.676
1.00
20.14
H


ATOM
1718
CG
LEU
11
142.591
26.256
44.045
1.00
23.62
H


ATOM
1719
CD1
LEU
11
143.477
25.265
43.309
1.00
18.85
H


ATOM
1720
CD2
LEU
11
142.110
27.357
43.100
1.00
23.67
H


ATOM
1721
C
LEU
11
139.306
25.400
43.357
1.00
44.95
H


ATOM
1722
O
LEU
11
138.867
24.257
43.519
1.00
54.63
H


ATOM
1723
N
VAL
12
139.210
26.057
42.207
1.00
41.04
H


ATOM
1724
CA
VAL
12
138.564
25.466
41.042
1.00
44.92
H


ATOM
1725
CB
VAL
12
137.198
26.116
40.760
1.00
55.40
H


ATOM
1726
CG1
VAL
12
136.166
25.600
41.736
1.00
60.33
H


ATOM
1727
CG2
VAL
12
137.324
27.631
40.849
1.00
67.40
H


ATOM
1728
C
VAL
12
139.428
25.687
39.824
1.00
38.99
H


ATOM
1729
O
VAL
12
140.065
26.727
39.702
1.00
23.16
H


ATOM
1730
N
LYS
13
139.451
24.705
38.928
1.00
56.02
H


ATOM
1731
CA
LYS
13
140.230
24.817
37.701
1.00
61.99
H


ATOM
1732
CB
LYS
13
140.577
23.430
37.154
1.00
47.53
H


ATOM
1733
CG
LYS
13
142.054
23.066
37.259
1.00
29.92
H


ATOM
1734
CD
LYS
13
142.364
21.831
36.416
1.00
39.21
H


ATOM
1735
CE
LYS
13
143.629
22.007
35.596
1.00
40.05
H


ATOM
1736
NZ
LYS
13
144.804
21.337
36.230
1.00
30.64
H


ATOM
1737
C
LYS
13
139.365
25.595
36.706
1.00
60.22
H


ATOM
1738
O
LYS
13
138.184
25.287
36.513
1.00
48.99
H


ATOM
1739
N
PRO
14
139.951
26.619
36.066
1.00
55.96
H


ATOM
1740
CD
PRO
14
141.359
27.003
36.236
1.00
55.17
H


ATOM
1741
CA
PRO
14
139.270
27.477
35.091
1.00
63.02
H


ATOM
1742
CB
PRO
14
140.412
28.234
34.404
1.00
59.73
H


ATOM
1743
CG
PRO
14
141.685
27.633
34.929
1.00
60.43
H


ATOM
1744
C
PRO
14
138.356
26.766
34.096
1.00
61.60
H


ATOM
1745
O
PRO
14
138.802
25.957
33.275
1.00
63.48
H


ATOM
1746
N
GLY
15
137.069
27.093
34.185
1.00
55.03
H


ATOM
1747
CA
GLY
15
136.077
26.503
33.307
1.00
55.23
H


ATOM
1748
C
GLY
15
134.961
25.815
34.071
1.00
49.69
H


ATOM
1749
O
GLY
15
133.800
25.865
33.670
1.00
45.93
H


ATOM
1750
N
THR
16
135.304
25.176
35.182
1.00
46.88
H


ATOM
1751
CA
THR
16
134.303
24.480
35.969
1.00
46.72
H


ATOM
1752
CB
THR
16
134.946
23.549
36.992
1.00
53.32
H


ATOM
1753
OG1
THR
16
135.914
24.275
37.758
1.00
63.69
H


ATOM
1754
CG2
THR
16
135.615
22.387
36.286
1.00
61.76
H


ATOM
1755
C
THR
16
133.361
25.415
36.700
1.00
42.84
H


ATOM
1756
O
THR
16
133.391
26.626
36.505
1.00
35.33
H


ATOM
1757
N
SER
17
132.535
24.829
37.559
1.00
46.34
H


ATOM
1758
CA
SER
17
131.543
25.570
38.320
1.00
44.29
H


ATOM
1759
CB
SER
17
130.190
25.458
37.621
1.00
43.09
H


ATOM
1760
OG
SER
17
130.349
25.482
36.211
1.00
37.03
H


ATOM
1761
C
SER
17
131.408
25.051
39.742
1.00
45.39
H


ATOM
1762
O
SER
17
131.124
23.872
39.956
1.00
51.18
H


ATOM
1763
N
VAL
18
131.599
25.938
40.713
1.00
43.96
H


ATOM
1764
CA
VAL
18
131.483
25.564
42.119
1.00
45.50
H


ATOM
1765
CB
VAL
18
132.723
25.989
42.932
1.00
47.04
H


ATOM
1766
CG1
VAL
18
132.979
27.469
42.756
1.00
45.76
H


ATOM
1767
CG2
VAL
18
132.513
25.665
44.400
1.00
36.17
H


ATOM
1768
C
VAL
18
130.257
26.218
42.742
1.00
46.62
H


ATOM
1769
O
VAL
18
129.772
27.240
42.258
1.00
24.82
H


ATOM
1770
N
ARG
19
129.758
25.610
43.813
1.00
53.11
H


ATOM
1771
CA
ARG
19
128.589
26.122
44.518
1.00
58.98
H


ATOM
1772
CB
ARG
19
127.414
25.157
44.354
1.00
48.17
H


ATOM
1773
CG
ARG
19
126.252
25.443
45.280
1.00
32.81
H


ATOM
1774
CD
ARG
19
125.479
24.167
45.605
1.00
61.51
H


ATOM
1775
NE
ARG
19
126.212
23.302
46.530
1.00
70.76
H


ATOM
1776
CZ
ARG
19
125.661
22.352
47.283
1.00
68.17
H


ATOM
1777
NH1
ARG
19
124.353
22.124
47.232
1.00
41.10
H


ATOM
1778
NH2
ARG
19
126.428
21.625
48.090
1.00
47.54
H


ATOM
1779
C
ARG
19
128.912
26.282
46.000
1.00
57.70
H


ATOM
1780
O
ARG
19
129.050
25.294
46.716
1.00
66.45
H


ATOM
1781
N
ILE
20
129.043
27.523
46.456
1.00
53.34
H


ATOM
1782
CA
ILE
20
129.345
27.780
47.860
1.00
52.34
H


ATOM
1783
CB
ILE
20
130.052
29.137
48.067
1.00
45.51
H


ATOM
1784
CG2
ILE
20
131.474
29.053
47.565
1.00
47.12
H


ATOM
1785
CG1
ILE
20
129.280
30.243
47.353
1.00
39.21
H


ATOM
1786
CD1
ILE
20
129.929
31.606
47.466
1.00
37.76
H


ATOM
1787
C
ILE
20
128.059
27.800
48.666
1.00
55.83
H


ATOM
1788
O
ILE
20
126.960
27.764
48.113
1.00
48.41
H


ATOM
1789
N
SER
21
128.208
27.860
49.981
1.00
65.06
H


ATOM
1790
CA
SER
21
127.058
27.892
50.863
1.00
70.97
H


ATOM
1791
CB
SER
21
126.810
26.503
51.468
1.00
72.43
H


ATOM
1792
OG
SER
21
127.562
26.314
52.654
1.00
60.74
H


ATOM
1793
C
SER
21
127.260
28.917
51.968
1.00
69.87
H


ATOM
1794
O
SER
21
128.288
29.599
52.026
1.00
70.39
H


ATOM
1795
N
CYS
22
126.262
29.013
52.839
1.00
69.21
H


ATOM
1796
CA
CYS
22
126.263
29.940
53.963
1.00
61.84
H


ATOM
1797
C
CYS
22
124.996
29.635
54.745
1.00
52.88
H


ATOM
1798
O
CYS
22
123.890
29.845
54.249
1.00
52.20
H


ATOM
1799
CB
CYS
22
126.233
31.379
53.461
1.00
52.16
H


ATOM
1800
SG
CYS
22
125.483
32.535
54.637
1.00
54.59
H


ATOM
1801
N
GLU
23
125.154
29.147
55.968
1.00
40.82
H


ATOM
1802
CA
GLU
23
123.998
28.783
56.767
1.00
43.24
H


ATOM
1803
CB
GLU
23
124.140
27.339
57.230
1.00
55.82
H


ATOM
1804
CG
GLU
23
125.578
26.915
57.456
1.00
76.19
H


ATOM
1805
CD
GLU
23
125.807
26.370
58.853
1.00
92.47
H


ATOM
1806
OE1
GLU
23
124.810
26.025
59.525
1.00
98.93
H


ATOM
1807
OE2
GLU
23
126.982
26.286
59.278
1.00
99.99
H


ATOM
1808
C
GLU
23
123.723
29.675
57.962
1.00
47.05
H


ATOM
1809
O
GLU
23
124.610
29.940
58.777
1.00
50.01
H


ATOM
1810
N
ALA
24
122.472
30.117
58.060
1.00
39.44
H


ATOM
1811
CA
ALA
24
122.022
30.980
59.143
1.00
43.91
H


ATOM
1812
CB
ALA
24
120.770
31.729
58.709
1.00
40.39
H


ATOM
1813
C
ALA
24
121.745
30.197
60.429
1.00
49.01
H


ATOM
1814
O
ALA
24
121.532
28.983
60.398
1.00
44.02
H


ATOM
1815
N
SER
25
121.752
30.909
61.555
1.00
60.65
H


ATOM
1816
CA
SER
25
121.504
30.321
62.872
1.00
64.10
H


ATOM
1817
CB
SER
25
122.557
29.250
63.192
1.00
76.12
H


ATOM
1818
OG
SER
25
123.782
29.512
62.529
1.00
87.44
H


ATOM
1819
C
SER
25
121.514
31.391
63.966
1.00
53.28
H


ATOM
1820
O
SER
25
122.520
32.068
64.188
1.00
50.41
H


ATOM
1821
N
GLY
26
120.384
31.533
64.651
1.00
46.94
H


ATOM
1822
CA
GLY
26
120.275
32.520
65.708
1.00
30.92
H


ATOM
1823
C
GLY
26
119.044
33.377
65.502
1.00
32.69
H


ATOM
1824
O
GLY
26
118.711
34.201
66.349
1.00
19.54
H


ATOM
1825
N
TYR
27
118.372
33.167
64.370
1.00
42.28
H


ATOM
1826
CA
TYR
27
117.163
33.909
64.012
1.00
45.58
H


ATOM
1827
CB
TYR
27
117.546
35.240
63.346
1.00
51.85
H


ATOM
1828
CG
TYR
27
118.025
35.116
61.907
1.00
59.01
H


ATOM
1829
CD1
TYR
27
117.155
35.345
60.838
1.00
52.27
H


ATOM
1830
CE1
TYR
27
117.595
35.261
59.514
1.00
42.40
H


ATOM
1831
CD2
TYR
27
119.355
34.794
61.614
1.00
52.66
H


ATOM
1832
CE2
TYR
27
119.804
34.710
60.293
1.00
46.26
H


ATOM
1833
CZ
TYR
27
118.919
34.947
59.250
1.00
50.61
H


ATOM
1834
OH
TYR
27
119.363
34.893
57.947
1.00
42.85
H


ATOM
1835
C
TYR
27
116.250
33.109
63.072
1.00
48.20
H


ATOM
1836
O
TYR
27
116.553
31.963
62.712
1.00
30.85
H


ATOM
1837
N
THR
28
115.131
33.722
62.684
1.00
47.35
H


ATOM
1838
CA
THR
28
114.176
33.088
61.776
1.00
41.83
H


ATOM
1839
CB
THR
28
112.786
33.737
61.871
1.00
39.96
H


ATOM
1840
OG1
THR
28
112.270
33.577
63.197
1.00
50.93
H


ATOM
1841
CG2
THR
28
111.830
33.084
60.878
1.00
45.16
H


ATOM
1842
C
THR
28
114.653
33.204
60.328
1.00
40.89
H


ATOM
1843
O
THR
28
114.619
34.280
59.739
1.00
31.24
H


ATOM
1844
N
PHE
29
115.095
32.084
59.766
1.00
41.61
H


ATOM
1845
CA
PHE
29
115.591
32.035
58.399
1.00
41.45
H


ATOM
1846
CB
PHE
29
115.946
30.593
58.034
1.00
30.51
H


ATOM
1847
CG
PHE
29
116.611
30.449
56.696
1.00
37.17
H


ATOM
1848
CD1
PHE
29
117.833
31.065
56.439
1.00
30.37
H


ATOM
1849
CD2
PHE
29
116.032
29.667
55.700
1.00
36.28
H


ATOM
1850
CE1
PHE
29
118.469
30.904
55.214
1.00
21.32
H


ATOM
1851
CE2
PHE
29
116.666
29.500
54.463
1.00
41.18
H


ATOM
1852
CZ
PHE
29
117.889
30.122
54.225
1.00
35.05
H


ATOM
1853
C
PHE
29
114.594
32.572
57.379
1.00
54.24
H


ATOM
1854
O
PHE
29
114.952
33.331
56.480
1.00
66.70
H


ATOM
1855
N
THR
30
113.342
32.164
57.527
1.00
55.99
H


ATOM
1856
CA
THR
30
112.276
32.557
56.615
1.00
52.36
H


ATOM
1857
CB
THR
30
111.052
31.652
56.824
1.00
53.76
H


ATOM
1858
OG1
THR
30
110.733
31.583
58.225
1.00
35.56
H


ATOM
1859
CG2
THR
30
111.357
30.244
56.304
1.00
44.72
H


ATOM
1860
C
THR
30
111.829
34.016
56.703
1.00
47.50
H


ATOM
1861
O
THR
30
110.914
34.426
55.996
1.00
53.11
H


ATOM
1862
N
SER
31
112.470
34.804
57.554
1.00
46.37
H


ATOM
1863
CA
SER
31
112.094
36.207
57.697
1.00
45.74
H


ATOM
1864
CB
SER
31
111.832
36.521
59.167
1.00
29.27
H


ATOM
1865
OG
SER
31
110.583
37.159
59.337
1.00
47.69
H


ATOM
1866
C
SER
31
113.143
37.175
57.150
1.00
47.78
H


ATOM
1867
O
SER
31
113.149
38.358
57.509
1.00
46.57
H


ATOM
1868
N
TYR
32
114.027
36.683
56.286
1.00
31.83
H


ATOM
1869
CA
TYR
32
115.068
37.530
55.720
1.00
30.55
H


ATOM
1870
CB
TYR
32
116.287
37.556
56.642
1.00
32.62
H


ATOM
1871
CG
TYR
32
116.075
38.248
57.963
1.00
37.51
H


ATOM
1872
CD1
TYR
32
115.494
37.579
59.031
1.00
33.89
H


ATOM
1873
CE1
TYR
32
115.358
38.184
60.266
1.00
35.72
H


ATOM
1874
CD2
TYR
32
116.510
39.556
58.166
1.00
39.03
H


ATOM
1875
CE2
TYR
32
116.378
40.173
59.401
1.00
40.85
H


ATOM
1876
CZ
TYR
32
115.803
39.476
60.449
1.00
43.40
H


ATOM
1877
OH
TYR
32
115.693
40.053
61.695
1.00
55.17
H


ATOM
1878
C
TYR
32
115.526
37.071
54.342
1.00
42.11
H


ATOM
1879
O
TYR
32
115.438
35.889
54.012
1.00
37.33
H


ATOM
1880
N
TYR
33
116.021
38.017
53.545
1.00
52.12
H


ATOM
1881
CA
TYR
33
116.544
37.721
52.210
1.00
50.02
H


ATOM
1882
CB
TYR
33
116.576
38.960
51.309
1.00
42.85
H


ATOM
1883
CG
TYR
33
115.263
39.601
50.944
1.00
58.80
H


ATOM
1884
CD1
TYR
33
114.637
39.313
49.730
1.00
66.77
H


ATOM
1885
CE1
TYR
33
113.466
39.968
49.345
1.00
69.61
H


ATOM
1886
CD2
TYR
33
114.683
40.558
51.771
1.00
76.77
H


ATOM
1887
CE2
TYR
33
113.513
41.220
51.398
1.00
80.20
H


ATOM
1888
CZ
TYR
33
112.909
40.921
50.185
1.00
74.03
H


ATOM
1889
OH
TYR
33
111.746
41.574
49.830
1.00
56.44
H


ATOM
1890
C
TYR
33
118.000
37.359
52.441
1.00
53.48
H


ATOM
1891
O
TYR
33
118.601
37.825
53.411
1.00
55.64
H


ATOM
1892
N
ILE
34
118.574
36.538
51.570
1.00
39.36
H


ATOM
1893
CA
ILE
34
119.991
36.237
51.705
1.00
36.92
H


ATOM
1894
CB
ILE
34
120.310
34.720
51.687
1.00
41.72
H


ATOM
1895
CG2
ILE
34
121.831
34.515
51.776
1.00
24.87
H


ATOM
1896
CG1
ILE
34
119.647
34.023
52.879
1.00
33.33
H


ATOM
1897
CD1
ILE
34
120.435
34.137
54.177
1.00
29.68
H


ATOM
1898
C
ILE
34
120.629
36.900
50.490
1.00
33.61
H


ATOM
1899
O
ILE
34
120.304
36.568
49.351
1.00
36.71
H


ATOM
1900
N
HIS
35
121.506
37.864
50.739
1.00
34.93
H


ATOM
1901
CA
HIS
35
122.185
38.573
49.666
1.00
29.41
H


ATOM
1902
CB
HIS
35
122.300
40.062
49.990
1.00
22.47
H


ATOM
1903
CG
HIS
35
120.983
40.768
50.047
1.00
28.34
H


ATOM
1904
CD2
HIS
35
120.082
40.885
51.049
1.00
17.42
H


ATOM
1905
ND1
HIS
35
120.446
41.427
48.965
1.00
34.24
H


ATOM
1906
CE1
HIS
35
119.266
41.924
49.298
1.00
35.85
H


ATOM
1907
NE2
HIS
35
119.023
41.609
50.556
1.00
28.90
H


ATOM
1908
C
HIS
35
123.574
38.000
49.482
1.00
24.58
H


ATOM
1909
O
HIS
35
124.156
37.437
50.403
1.00
40.74
H


ATOM
1910
N
TRP
36
124.104
38.149
48.282
1.00
18.85
H


ATOM
1911
CA
TRP
36
125.430
37.664
47.984
1.00
25.49
H


ATOM
1912
CB
TRP
36
125.334
36.410
47.104
1.00
38.00
H


ATOM
1913
CG
TRP
36
125.226
35.122
47.882
1.00
42.56
H


ATOM
1914
CD2
TRP
36
126.288
34.433
48.564
1.00
28.82
H


ATOM
1915
CE2
TRP
36
125.730
33.274
49.146
1.00
18.67
H


ATOM
1916
CE3
TRP
36
127.658
34.687
48.737
1.00
34.82
H


ATOM
1917
CD1
TRP
36
124.097
34.368
48.075
1.00
40.81
H


ATOM
1918
NE1
TRP
36
124.394
33.257
48.834
1.00
30.56
H


ATOM
1919
CZ2
TRP
36
126.492
32.368
49.889
1.00
44.00
H


ATOM
1920
CZ3
TRP
36
128.418
33.786
49.479
1.00
41.71
H


ATOM
1921
CH2
TRP
36
127.832
32.641
50.045
1.00
49.64
H


ATOM
1922
C
TRP
36
126.189
38.784
47.269
1.00
22.21
H


ATOM
1923
O
TRP
36
125.658
39.410
46.352
1.00
20.33
H


ATOM
1924
N
VAL
37
127.420
39.044
47.703
1.00
21.22
H


ATOM
1925
CA
VAL
37
128.237
40.085
47.089
1.00
29.63
H


ATOM
1926
CB
VAL
37
128.157
41.414
47.887
1.00
30.69
H


ATOM
1927
CG1
VAL
37
126.874
41.453
48.701
1.00
7.82
H


ATOM
1928
CG2
VAL
37
129.366
41.569
48.780
1.00
16.02
H


ATOM
1929
C
VAL
37
129.693
39.641
46.984
1.00
33.49
H


ATOM
1930
O
VAL
37
130.223
39.017
47.900
1.00
17.14
H


ATOM
1931
N
LYS
38
130.330
39.970
45.862
1.00
43.70
H


ATOM
1932
CA
LYS
38
131.720
39.591
45.619
1.00
47.72
H


ATOM
1933
CB
LYS
38
131.849
38.932
44.233
1.00
55.60
H


ATOM
1934
CG
LYS
38
132.592
39.756
43.175
1.00
55.19
H


ATOM
1935
CD
LYS
38
133.109
38.890
42.022
1.00
50.50
H


ATOM
1936
CE
LYS
38
131.967
38.347
41.155
1.00
51.42
H


ATOM
1937
NZ
LYS
38
132.372
37.162
40.335
1.00
38.13
H


ATOM
1938
C
LYS
38
132.699
40.757
45.732
1.00
48.64
H


ATOM
1939
O
LYS
38
132.467
41.837
45.189
1.00
52.35
H


ATOM
1940
N
GLN
39
133.798
40.525
46.439
1.00
38.36
H


ATOM
1941
CA
GLN
39
134.819
41.545
46.621
1.00
30.47
H


ATOM
1942
CB
GLN
39
134.969
41.879
48.103
1.00
40.49
H


ATOM
1943
CG
GLN
39
136.078
42.863
48.409
1.00
23.44
H


ATOM
1944
CD
GLN
39
135.965
43.424
49.803
1.00
27.61
H


ATOM
1945
OE1
GLN
39
135.386
44.485
50.004
1.00
26.42
H


ATOM
1946
NE2
GLN
39
136.517
42.709
50.781
1.00
31.89
H


ATOM
1947
C
GLN
39
136.142
41.029
46.074
1.00
29.22
H


ATOM
1948
O
GLN
39
136.781
40.160
46.672
1.00
19.00
H


ATOM
1949
N
ARG
40
136.548
41.560
44.928
1.00
41.18
H


ATOM
1950
CA
ARG
40
137.794
41.139
44.310
1.00
48.75
H


ATOM
1951
CB
ARG
40
137.805
41.496
42.819
1.00
47.54
H


ATOM
1952
CG
ARG
40
137.203
40.422
41.923
1.00
39.67
H


ATOM
1953
CD
ARG
40
137.409
40.741
40.448
1.00
63.74
H


ATOM
1954
NE
ARG
40
138.762
40.415
39.997
1.00
66.96
H


ATOM
1955
CZ
ARG
40
139.215
39.178
39.816
1.00
59.13
H


ATOM
1956
NH1
ARG
40
138.426
38.137
40.046
1.00
49.49
H


ATOM
1957
NH2
ARG
40
140.459
38.982
39.400
1.00
64.52
H


ATOM
1958
C
ARG
40
138.971
41.791
45.024
1.00
49.05
H


ATOM
1959
O
ARG
40
138.955
42.989
45.312
1.00
41.67
H


ATOM
1960
N
PRO
41
140.007
40.996
45.329
1.00
49.95
H


ATOM
1961
CD
PRO
41
140.102
39.557
45.022
1.00
60.26
H


ATOM
1962
CA
PRO
41
141.200
41.485
46.013
1.00
40.75
H


ATOM
1963
CB
PRO
41
142.268
40.476
45.625
1.00
50.53
H


ATOM
1964
CG
PRO
41
141.516
39.202
45.415
1.00
62.82
H


ATOM
1965
C
PRO
41
141.558
42.888
45.576
1.00
38.02
H


ATOM
1966
O
PRO
41
141.769
43.147
44.392
1.00
47.09
H


ATOM
1967
N
GLY
42
141.614
43.794
46.542
1.00
36.72
H


ATOM
1968
CA
GLY
42
141.952
45.170
46.241
1.00
31.35
H


ATOM
1969
C
GLY
42
140.726
45.981
45.900
1.00
40.05
H


ATOM
1970
O
GLY
42
140.468
47.014
46.514
1.00
41.40
H


ATOM
1971
N
GLN
43
139.975
45.502
44.913
1.00
58.75
H


ATOM
1972
CA
GLN
43
138.752
46.163
44.468
1.00
67.06
H


ATOM
1973
CB
GLN
43
138.192
45.449
43.227
1.00
64.21
H


ATOM
1974
CG
GLN
43
137.035
46.178
42.540
1.00
71.56
H


ATOM
1975
CD
GLN
43
135.900
45.251
42.130
1.00
71.51
H


ATOM
1976
OE1
GLN
43
135.330
44.540
42.963
1.00
67.00
H


ATOM
1977
NE2
GLN
43
135.566
45.255
40.842
1.00
53.00
H


ATOM
1978
C
GLN
43
137.701
46.158
45.581
1.00
68.90
H


ATOM
1979
O
GLN
43
137.782
45.366
46.521
1.00
72.87
H


ATOM
1980
N
GLY
44
136.723
47.055
45.471
1.00
67.47
H


ATOM
1981
CA
GLY
44
135.661
47.119
46.458
1.00
54.03
H


ATOM
1982
C
GLY
44
134.746
45.927
46.268
1.00
52.55
H


ATOM
1983
O
GLY
44
135.191
44.881
45.794
1.00
65.13
H


ATOM
1984
N
LEU
45
133.474
46.062
46.623
1.00
38.94
H


ATOM
1985
CA
LEU
45
132.552
44.946
46.456
1.00
50.12
H


ATOM
1986
CB
LEU
45
132.197
44.339
47.819
1.00
44.25
H


ATOM
1987
CG
LEU
45
131.306
45.109
48.790
1.00
36.87
H


ATOM
1988
CD1
LEU
45
131.661
44.692
50.212
1.00
34.38
H


ATOM
1989
CD2
LEU
45
131.480
46.605
48.597
1.00
31.30
H


ATOM
1990
C
LEU
45
131.283
45.315
45.689
1.00
47.35
H


ATOM
1991
O
LEU
45
130.876
46.475
45.656
1.00
52.97
H


ATOM
1992
N
GLU
46
130.673
44.311
45.066
1.00
38.18
H


ATOM
1993
CA
GLU
46
129.468
44.497
44.275
1.00
32.36
H


ATOM
1994
CB
GLU
46
129.779
44.312
42.787
1.00
54.58
H


ATOM
1995
CG
GLU
46
131.106
43.601
42.502
1.00
70.14
H


ATOM
1996
CD
GLU
46
131.544
43.722
41.050
1.00
59.62
H


ATOM
1997
OE1
GLU
46
132.769
43.760
40.796
1.00
60.49
H


ATOM
1998
OE2
GLU
46
130.660
43.778
40.171
1.00
62.64
H


ATOM
1999
C
GLU
46
128.400
43.506
44.686
1.00
33.28
H


ATOM
2000
O
GLU
46
128.697
42.477
45.293
1.00
22.50
H


ATOM
2001
N
TRP
47
127.157
43.818
44.329
1.00
30.17
H


ATOM
2002
CA
TRP
47
126.010
42.982
44.661
1.00
39.51
H


ATOM
2003
CB
TRP
47
124.794
43.881
44.906
1.00
50.31
H


ATOM
2004
CG
TRP
47
123.538
43.163
45.304
1.00
46.71
H


ATOM
2005
CD2
TRP
47
122.417
42.881
44.459
1.00
46.71
H


ATOM
2006
CE2
TRP
47
121.440
42.259
45.262
1.00
40.21
H


ATOM
2007
CE3
TRP
47
122.148
43.096
43.104
1.00
44.16
H


ATOM
2008
CD1
TRP
47
123.204
42.709
46.547
1.00
44.85
H


ATOM
2009
NE1
TRP
47
121.942
42.165
46.533
1.00
44.22
H


ATOM
2010
CZ2
TRP
47
120.205
41.852
44.750
1.00
48.68
H


ATOM
2011
CZ3
TRP
47
120.924
42.689
42.597
1.00
48.92
H


ATOM
2012
CH2
TRP
47
119.969
42.073
43.420
1.00
39.77
H


ATOM
2013
C
TRP
47
125.692
41.936
43.592
1.00
45.09
H


ATOM
2014
O
TRP
47
125.359
42.260
42.449
1.00
45.19
H


ATOM
2015
N
ILE
48
125.800
40.671
43.981
1.00
45.61
H


ATOM
2016
CA
ILE
48
125.529
39.562
43.080
1.00
37.68
H


ATOM
2017
CB
ILE
48
126.185
38.266
43.601
1.00
25.87
H


ATOM
2018
CG2
ILE
48
125.479
37.039
43.036
1.00
23.09
H


ATOM
2019
CG1
ILE
48
127.657
38.249
43.202
1.00
15.27
H


ATOM
2020
CD1
ILE
48
128.449
37.133
43.847
1.00
20.12
H


ATOM
2021
C
ILE
48
124.028
39.356
42.936
1.00
35.05
H


ATOM
2022
O
ILE
48
123.461
39.591
41.870
1.00
41.96
H


ATOM
2023
N
GLY
49
123.388
38.918
44.013
1.00
32.61
H


ATOM
2024
CA
GLY
49
121.953
38.689
43.986
1.00
30.75
H


ATOM
2025
C
GLY
49
121.432
38.298
45.359
1.00
38.37
H


ATOM
2026
O
GLY
49
122.161
38.327
46.349
1.00
41.94
H


ATOM
2027
N
CYS
50
120.163
37.931
45.431
1.00
37.54
H


ATOM
2028
CA
CYS
50
119.587
37.540
46.705
1.00
38.40
H


ATOM
2029
CB
CYS
50
119.095
38.774
47.462
1.00
51.77
H


ATOM
2030
SG
CYS
50
117.500
39.407
46.877
1.00
50.59
H


ATOM
2031
C
CYS
50
118.438
36.577
46.500
1.00
42.42
H


ATOM
2032
O
CYS
50
117.835
36.535
45.431
1.00
50.09
H


ATOM
2033
N
ILE
51
118.146
35.804
47.537
1.00
42.92
H


ATOM
2034
CA
ILE
51
117.059
34.837
47.502
1.00
37.44
H


ATOM
2035
CB
ILE
51
117.596
33.380
47.377
1.00
28.69
H


ATOM
2036
CG2
ILE
51
118.648
33.111
48.440
1.00
29.15
H


ATOM
2037
CG1
ILE
51
116.448
32.382
47.521
1.00
22.13
H


ATOM
2038
CD1
ILE
51
115.840
31.963
46.201
1.00
43.28
H


ATOM
2039
C
ILE
51
116.277
34.989
48.800
1.00
42.00
H


ATOM
2040
O
ILE
51
116.861
35.219
49.868
1.00
34.19
H


ATOM
2041
N
TYR
52
114.957
34.886
48.706
1.00
38.73
H


ATOM
2042
CA
TYR
52
114.124
35.013
49.888
1.00
34.95
H


ATOM
2043
CB
TYR
52
112.954
35.960
49.643
1.00
26.98
H


ATOM
2044
CG
TYR
52
112.041
36.049
50.840
1.00
28.39
H


ATOM
2045
CD1
TYR
52
112.571
36.155
52.132
1.00
31.39
H


ATOM
2046
CE1
TYR
52
111.752
36.193
53.244
1.00
43.44
H


ATOM
2047
CD2
TYR
52
110.660
35.986
50.695
1.00
19.91
H


ATOM
2048
CE2
TYR
52
109.821
36.022
51.800
1.00
43.98
H


ATOM
2049
CZ
TYR
52
110.376
36.124
53.075
1.00
56.97
H


ATOM
2050
OH
TYR
52
109.556
36.153
54.178
1.00
67.54
H


ATOM
2051
C
TYR
52
113.578
33.674
50.350
1.00
39.53
H


ATOM
2052
O
TYR
52
112.611
33.161
49.791
1.00
45.44
H


ATOM
2053
N
PRO
53
114.191
33.092
51.386
1.00
46.32
H


ATOM
2054
CD
PRO
53
115.347
33.610
52.137
1.00
58.13
H


ATOM
2055
CA
PRO
53
113.728
31.803
51.900
1.00
36.81
H


ATOM
2056
CB
PRO
53
114.599
31.566
53.126
1.00
38.59
H


ATOM
2057
CG
PRO
53
115.817
32.404
52.894
1.00
59.97
H


ATOM
2058
C
PRO
53
112.279
31.971
52.275
1.00
35.25
H


ATOM
2059
O
PRO
53
111.926
32.927
52.959
1.00
37.50
H


ATOM
2060
N
GLY
54
111.441
31.055
51.815
1.00
43.54
H


ATOM
2061
CA
GLY
54
110.027
31.142
52.120
1.00
53.47
H


ATOM
2062
C
GLY
54
109.200
31.023
50.860
1.00
58.70
H


ATOM
2063
O
GLY
54
108.726
29.936
50.530
1.00
74.82
H


ATOM
2064
N
ASN
55
109.021
32.132
50.150
1.00
48.62
H


ATOM
2065
CA
ASN
55
108.248
32.116
48.917
1.00
45.89
H


ATOM
2066
CB
ASN
55
107.602
33.482
48.675
1.00
46.66
H


ATOM
2067
CG
ASN
55
108.344
34.312
47.658
1.00
46.59
H


ATOM
2068
OD1
ASN
55
109.183
35.138
48.009
1.00
50.93
H


ATOM
2069
ND2
ASN
55
108.027
34.107
46.384
1.00
65.32
H


ATOM
2070
C
ASN
55
109.177
31.740
47.777
1.00
59.93
H


ATOM
2071
O
ASN
55
108.738
31.536
46.645
1.00
70.19
H


ATOM
2072
N
VAL
56
110.466
31.644
48.110
1.00
68.48
H


ATOM
2073
CA
VAL
56
111.541
31.269
47.182
1.00
68.32
H


ATOM
2074
CB
VAL
56
111.181
29.960
46.416
1.00
73.69
H


ATOM
2075
CG1
VAL
56
110.739
30.271
44.989
1.00
75.39
H


ATOM
2076
CG2
VAL
56
112.377
29.021
46.415
1.00
57.36
H


ATOM
2077
C
VAL
56
111.958
32.352
46.182
1.00
64.26
H


ATOM
2078
O
VAL
56
112.859
32.142
45.366
1.00
50.69
H


ATOM
2079
N
ASN
57
111.307
33.511
46.258
1.00
70.60
H


ATOM
2080
CA
ASN
57
111.610
34.622
45.366
1.00
62.78
H


ATOM
2081
CB
ASN
57
110.996
35.913
45.898
1.00
76.22
H


ATOM
2082
CG
ASN
57
109.868
36.422
45.032
1.00
82.20
H


ATOM
2083
OD1
ASN
57
109.018
37.191
45.486
1.00
72.33
H


ATOM
2084
ND2
ASN
57
109.849
35.989
43.772
1.00
72.74
H


ATOM
2085
C
ASN
57
113.110
34.795
45.242
1.00
63.32
H


ATOM
2086
O
ASN
57
113.857
34.515
46.181
1.00
68.16
H


ATOM
2087
N
THR
58
113.553
35.267
44.084
1.00
54.53
H


ATOM
2088
CA
THR
58
114.974
35.465
43.859
1.00
52.32
H


ATOM
2089
CB
THR
58
115.597
34.191
43.279
1.00
48.24
H


ATOM
2090
OG1
THR
58
116.806
34.523
42.594
1.00
45.77
H


ATOM
2091
CG2
THR
58
114.628
33.516
42.314
1.00
53.99
H


ATOM
2092
C
THR
58
115.243
36.641
42.921
1.00
52.31
H


ATOM
2093
O
THR
58
114.436
36.935
42.039
1.00
59.83
H


ATOM
2094
N
ASN
59
116.375
37.313
43.117
1.00
40.59
H


ATOM
2095
CA
ASN
59
116.730
38.462
42.290
1.00
38.18
H


ATOM
2096
CB
ASN
59
116.330
39.758
42.998
1.00
53.76
H


ATOM
2097
CG
ASN
59
114.928
40.217
42.632
1.00
53.44
H


ATOM
2098
OD1
ASN
59
114.712
40.809
41.572
1.00
58.59
H


ATOM
2099
ND2
ASN
59
113.970
39.951
43.512
1.00
44.63
H


ATOM
2100
C
ASN
59
118.217
38.505
41.970
1.00
30.27
H


ATOM
2101
O
ASN
59
119.049
38.423
42.873
1.00
35.55
H


ATOM
2102
N
TYR
60
118.544
38.661
40.687
1.00
19.72
H


ATOM
2103
CA
TYR
60
119.935
38.710
40.243
1.00
29.52
H


ATOM
2104
CB
TYR
60
120.213
37.596
39.227
1.00
27.47
H


ATOM
2105
CG
TYR
60
119.809
36.215
39.675
1.00
25.29
H


ATOM
2106
CD1
TYR
60
120.762
35.233
39.929
1.00
18.79
H


ATOM
2107
CE1
TYR
60
120.382
33.951
40.325
1.00
40.31
H


ATOM
2108
CD2
TYR
60
118.468
35.883
39.825
1.00
40.64
H


ATOM
2109
CE2
TYR
60
118.077
34.611
40.217
1.00
47.25
H


ATOM
2110
CZ
TYR
60
119.033
33.647
40.469
1.00
51.22
H


ATOM
2111
OH
TYR
60
118.621
32.391
40.883
1.00
56.18
H


ATOM
2112
C
TYR
60
120.367
40.034
39.618
1.00
25.63
H


ATOM
2113
O
TYR
60
119.574
40.746
39.008
1.00
17.78
H


ATOM
2114
N
ASN
61
121.645
40.354
39.789
1.00
31.77
H


ATOM
2115
CA
ASN
61
122.224
41.551
39.213
1.00
30.42
H


ATOM
2116
CB
ASN
61
123.542
41.891
39.907
1.00
26.96
H


ATOM
2117
CG
ASN
61
124.176
43.155
39.367
1.00
28.98
H


ATOM
2118
OD1
ASN
61
124.721
43.964
40.120
1.00
39.03
H


ATOM
2119
ND2
ASN
61
124.105
43.335
38.052
1.00
39.88
H


ATOM
2120
C
ASN
61
122.480
41.102
37.787
1.00
42.86
H


ATOM
2121
O
ASN
61
123.062
40.041
37.571
1.00
56.00
H


ATOM
2122
N
GLU
62
122.045
41.889
36.812
1.00
46.51
H


ATOM
2123
CA
GLU
62
122.224
41.498
35.424
1.00
52.07
H


ATOM
2124
CB
GLU
62
121.511
42.490
34.504
1.00
57.39
H


ATOM
2125
CG
GLU
62
120.513
41.839
33.531
1.00
76.62
H


ATOM
2126
CD
GLU
62
119.280
41.242
34.214
1.00
81.14
H


ATOM
2127
OE1
GLU
62
119.442
40.484
35.197
1.00
79.43
H


ATOM
2128
OE2
GLU
62
118.148
41.528
33.759
1.00
70.90
H


ATOM
2129
C
GLU
62
123.678
41.327
35.000
1.00
53.65
H


ATOM
2130
O
GLU
62
123.953
40.991
33.847
1.00
75.05
H


ATOM
2131
N
LYS
63
124.610
41.543
35.922
1.00
36.05
H


ATOM
2132
CA
LYS
63
126.025
41.379
35.604
1.00
35.02
H


ATOM
2133
CB
LYS
63
126.862
42.421
36.353
1.00
26.34
H


ATOM
2134
CG
LYS
63
127.215
43.653
35.532
1.00
49.15
H


ATOM
2135
CD
LYS
63
126.773
44.948
36.217
1.00
45.62
H


ATOM
2136
CE
LYS
63
127.938
45.619
36.920
1.00
40.94
H


ATOM
2137
NZ
LYS
63
128.652
44.625
37.772
1.00
39.59
H


ATOM
2138
C
LYS
63
126.506
39.969
35.961
1.00
40.58
H


ATOM
2139
O
LYS
63
127.618
39.576
35.611
1.00
45.93
H


ATOM
2140
N
PHE
64
125.657
39.214
36.658
1.00
47.98
H


ATOM
2141
CA
PHE
64
125.975
37.849
37.073
1.00
42.86
H


ATOM
2142
CB
PHE
64
126.263
37.789
38.579
1.00
42.85
H


ATOM
2143
CG
PHE
64
127.177
38.872
39.068
1.00
44.03
H


ATOM
2144
CD1
PHE
64
128.538
38.831
38.791
1.00
50.59
H


ATOM
2145
CD2
PHE
64
126.680
39.929
39.817
1.00
38.08
H


ATOM
2146
CE1
PHE
64
129.388
39.830
39.255
1.00
45.27
H


ATOM
2147
CE2
PHE
64
127.524
40.933
40.284
1.00
43.09
H


ATOM
2148
CZ
PHE
64
128.877
40.883
40.004
1.00
31.11
H


ATOM
2149
C
PHE
64
124.823
36.908
36.768
1.00
43.11
H


ATOM
2150
O
PHE
64
124.560
35.988
37.535
1.00
47.33
H


ATOM
2151
N
LYS
65
124.148
37.128
35.644
1.00
55.21
H


ATOM
2152
CA
LYS
65
123.005
36.302
35.259
1.00
59.07
H


ATOM
2153
CB
LYS
65
122.383
36.806
33.949
1.00
45.97
H


ATOM
2154
CG
LYS
65
123.146
37.914
33.251
1.00
49.55
H


ATOM
2155
CD
LYS
65
124.165
37.363
32.270
1.00
46.70
H


ATOM
2156
CE
LYS
65
125.116
38.461
31.811
1.00
50.30
H


ATOM
2157
NZ
LYS
65
125.548
38.271
30.398
1.00
58.93
H


ATOM
2158
C
LYS
65
123.279
34.802
35.118
1.00
67.16
H


ATOM
2159
O
LYS
65
122.598
33.984
35.741
1.00
70.12
H


ATOM
2160
N
ASP
66
124.267
34.440
34.304
1.00
69.41
H


ATOM
2161
CA
ASP
66
124.579
33.031
34.073
1.00
66.83
H


ATOM
2162
CB
ASP
66
125.508
32.898
32.872
1.00
66.07
H


ATOM
2163
CG
ASP
66
124.747
32.712
31.580
1.00
66.45
H


ATOM
2164
OD1
ASP
66
124.869
31.628
30.973
1.00
66.35
H


ATOM
2165
OD2
ASP
66
124.023
33.649
31.177
1.00
50.12
H


ATOM
2166
C
ASP
66
125.168
32.265
35.251
1.00
70.25
H


ATOM
2167
O
ASP
66
124.514
31.365
35.792
1.00
72.29
H


ATOM
2168
N
LYS
67
126.399
32.605
35.637
1.00
62.38
H


ATOM
2169
CA
LYS
67
127.071
31.934
36.750
1.00
49.02
H


ATOM
2170
CB
LYS
67
128.374
32.653
37.115
1.00
25.39
H


ATOM
2171
CG
LYS
67
128.541
34.034
36.489
1.00
48.63
H


ATOM
2172
CD
LYS
67
129.958
34.579
36.675
1.00
21.16
H


ATOM
2173
CE
LYS
67
130.912
34.018
35.624
1.00
43.17
H


ATOM
2174
NZ
LYS
67
132.251
33.618
36.179
1.00
34.20
H


ATOM
2175
C
LYS
67
126.178
31.887
37.977
1.00
59.48
H


ATOM
2176
O
LYS
67
125.721
30.816
38.390
1.00
51.12
H


ATOM
2177
N
ALA
68
125.929
33.066
38.545
1.00
67.14
H


ATOM
2178
CA
ALA
68
125.104
33.220
39.741
1.00
54.74
H


ATOM
2179
CB
ALA
68
125.017
34.688
40.108
1.00
54.84
H


ATOM
2180
C
ALA
68
123.701
32.631
39.631
1.00
49.40
H


ATOM
2181
O
ALA
68
122.851
33.136
38.896
1.00
58.08
H


ATOM
2182
N
THR
69
123.473
31.564
40.387
1.00
32.05
H


ATOM
2183
CA
THR
69
122.192
30.874
40.429
1.00
37.56
H


ATOM
2184
CB
THR
69
122.284
29.541
39.655
1.00
35.94
H


ATOM
2185
OG1
THR
69
122.392
29.821
38.253
1.00
40.10
H


ATOM
2186
CG2
THR
69
121.065
28.676
39.908
1.00
26.92
H


ATOM
2187
C
THR
69
121.915
30.626
41.914
1.00
43.54
H


ATOM
2188
O
THR
69
122.595
29.823
42.550
1.00
40.52
H


ATOM
2189
N
LEU
70
120.924
31.319
42.469
1.00
45.97
H


ATOM
2190
CA
LEU
70
120.622
31.189
43.893
1.00
46.62
H


ATOM
2191
CB
LEU
70
120.166
32.536
44.445
1.00
47.28
H


ATOM
2192
CG
LEU
70
121.305
33.331
45.091
1.00
55.81
H


ATOM
2193
CD1
LEU
70
121.133
34.805
44.767
1.00
43.73
H


ATOM
2194
CD2
LEU
70
121.322
33.094
46.611
1.00
43.94
H


ATOM
2195
C
LEU
70
119.636
30.107
44.312
1.00
43.94
H


ATOM
2196
O
LEU
70
118.496
30.070
43.849
1.00
42.32
H


ATOM
2197
N
ILE
71
120.103
29.247
45.218
1.00
44.21
H


ATOM
2198
CA
ILE
71
119.333
28.125
45.755
1.00
49.89
H


ATOM
2199
CB
ILE
71
120.026
26.770
45.430
1.00
51.60
H


ATOM
2200
CG2
ILE
71
119.259
25.616
46.072
1.00
29.06
H


ATOM
2201
CG1
ILE
71
120.107
26.573
43.911
1.00
54.43
H


ATOM
2202
CD1
ILE
71
121.184
27.402
43.222
1.00
43.28
H


ATOM
2203
C
ILE
71
119.203
28.255
47.278
1.00
52.79
H


ATOM
2204
O
ILE
71
120.103
28.779
47.939
1.00
62.18
H


ATOM
2205
N
VAL
72
118.093
27.768
47.830
1.00
50.92
H


ATOM
2206
CA
VAL
72
117.837
27.843
49.269
1.00
52.77
H


ATOM
2207
CB
VAL
72
116.551
28.677
49.549
1.00
47.96
H


ATOM
2208
CG1
VAL
72
115.313
27.828
49.317
1.00
44.08
H


ATOM
2209
CG2
VAL
72
116.569
29.224
50.967
1.00
43.63
H


ATOM
2210
C
VAL
72
117.687
26.437
49.861
1.00
56.26
H


ATOM
2211
O
VAL
72
117.627
25.457
49.123
1.00
65.48
H


ATOM
2212
N
ASP
73
117.638
26.344
51.191
1.00
56.09
H


ATOM
2213
CA
ASP
73
117.492
25.064
51.891
1.00
50.35
H


ATOM
2214
CB
ASP
73
118.848
24.365
51.995
1.00
53.65
H


ATOM
2215
CG
ASP
73
118.721
22.907
52.384
1.00
57.99
H


ATOM
2216
OD1
ASP
73
118.119
22.616
53.443
1.00
57.92
H


ATOM
2217
OD2
ASP
73
119.229
22.055
51.625
1.00
61.79
H


ATOM
2218
C
ASP
73
116.932
25.313
53.290
1.00
50.51
H


ATOM
2219
O
ASP
73
117.664
25.288
54.280
1.00
43.10
H


ATOM
2220
N
THR
74
115.624
25.535
53.352
1.00
56.54
H


ATOM
2221
CA
THR
74
114.913
25.836
54.593
1.00
66.24
H


ATOM
2222
CB
THR
74
113.421
26.020
54.296
1.00
71.27
H


ATOM
2223
OG1
THR
74
113.183
25.793
52.901
1.00
71.81
H


ATOM
2224
CG2
THR
74
112.979
27.430
54.659
1.00
81.34
H


ATOM
2225
C
THR
74
115.054
24.876
55.785
1.00
71.66
H


ATOM
2226
O
THR
74
114.662
25.220
56.902
1.00
67.59
H


ATOM
2227
N
SER
75
115.610
23.689
55.559
1.00
75.40
H


ATOM
2228
CA
SER
75
115.775
22.712
56.634
1.00
69.25
H


ATOM
2229
CB
SER
75
115.833
21.292
56.063
1.00
76.48
H


ATOM
2230
OG
SER
75
116.615
20.445
56.891
1.00
75.33
H


ATOM
2231
C
SER
75
117.024
22.961
57.474
1.00
60.40
H


ATOM
2232
O
SER
75
116.949
23.066
58.703
1.00
51.38
H


ATOM
2233
N
SER
76
118.171
23.040
56.807
1.00
45.77
H


ATOM
2234
CA
SER
76
119.435
23.282
57.498
1.00
44.97
H


ATOM
2235
CB
SER
76
120.585
22.601
56.749
1.00
51.28
H


ATOM
2236
OG
SER
76
120.106
21.807
55.676
1.00
43.07
H


ATOM
2237
C
SER
76
119.691
24.781
57.589
1.00
34.83
H


ATOM
2238
O
SER
76
120.755
25.221
58.008
1.00
28.11
H


ATOM
2239
N
ASN
77
118.697
25.563
57.194
1.00
46.96
H


ATOM
2240
CA
ASN
77
118.826
27.009
57.222
1.00
38.99
H


ATOM
2241
CB
ASN
77
118.847
27.513
58.665
1.00
41.25
H


ATOM
2242
CG
ASN
77
117.459
27.785
59.206
1.00
39.52
H


ATOM
2243
OD1
ASN
77
116.470
27.731
58.468
1.00
40.29
H


ATOM
2244
ND2
ASN
77
117.377
28.082
60.496
1.00
23.39
H


ATOM
2245
C
ASN
77
120.111
27.395
56.515
1.00
34.49
H


ATOM
2246
O
ASN
77
120.971
28.067
57.077
1.00
45.69
H


ATOM
2247
N
THR
78
120.240
26.944
55.275
1.00
39.07
H


ATOM
2248
CA
THR
78
121.417
27.244
54.481
1.00
43.99
H


ATOM
2249
CB
THR
78
122.340
26.001
54.336
1.00
47.54
H


ATOM
2250
OG1
THR
78
121.955
25.239
53.189
1.00
23.72
H


ATOM
2251
CG2
THR
78
122.244
25.122
55.568
1.00
19.39
H


ATOM
2252
C
THR
78
121.030
27.761
53.100
1.00
35.58
H


ATOM
2253
O
THR
78
120.107
27.255
52.465
1.00
41.21
H


ATOM
2254
N
ALA
79
121.738
28.791
52.658
1.00
32.09
H


ATOM
2255
CA
ALA
79
121.498
29.399
51.361
1.00
45.71
H


ATOM
2256
CB
ALA
79
121.265
30.903
51.520
1.00
20.04
H


ATOM
2257
C
ALA
79
122.734
29.148
50.509
1.00
54.97
H


ATOM
2258
O
ALA
79
123.854
29.447
50.927
1.00
71.91
H


ATOM
2259
N
TYR
80
122.550
28.589
49.321
1.00
37.02
H


ATOM
2260
CA
TYR
80
123.697
28.329
48.480
1.00
32.51
H


ATOM
2261
CB
TYR
80
123.635
26.921
47.920
1.00
27.41
H


ATOM
2262
CG
TYR
80
123.465
25.846
48.950
1.00
28.36
H


ATOM
2263
CD1
TYR
80
124.539
25.049
49.332
1.00
30.12
H


ATOM
2264
CE1
TYR
80
124.371
24.000
50.232
1.00
47.45
H


ATOM
2265
CD2
TYR
80
122.212
25.575
49.495
1.00
29.76
H


ATOM
2266
CE2
TYR
80
122.028
24.529
50.394
1.00
40.26
H


ATOM
2267
CZ
TYR
80
123.111
23.742
50.759
1.00
45.55
H


ATOM
2268
OH
TYR
80
122.933
22.695
51.643
1.00
45.67
H


ATOM
2269
C
TYR
80
123.819
29.307
47.327
1.00
47.49
H


ATOM
2270
O
TYR
80
122.870
30.010
46.974
1.00
54.32
H


ATOM
2271
N
MET
81
125.012
29.328
46.744
1.00
58.24
H


ATOM
2272
CA
MET
81
125.340
30.175
45.609
1.00
51.28
H


ATOM
2273
CB
MET
81
126.117
31.401
46.073
1.00
47.13
H


ATOM
2274
CG
MET
81
127.250
31.794
45.151
1.00
49.47
H


ATOM
2275
SD
MET
81
126.890
33.310
44.268
1.00
52.80
H


ATOM
2276
CE
MET
81
127.508
34.508
45.421
1.00
52.92
H


ATOM
2277
C
MET
81
126.220
29.327
44.699
1.00
51.47
H


ATOM
2278
O
MET
81
127.342
28.976
45.065
1.00
48.72
H


ATOM
2279
N
GLN
82
125.707
28.981
43.525
1.00
46.87
H


ATOM
2280
CA
GLN
82
126.476
28.175
42.595
1.00
45.38
H


ATOM
2281
CB
GLN
82
125.589
27.113
41.943
1.00
71.53
H


ATOM
2282
CG
GLN
82
125.978
26.739
40.511
1.00
89.74
H


ATOM
2283
CD
GLN
82
127.278
25.955
40.433
1.00
96.73
H


ATOM
2284
OE1
GLN
82
127.925
25.699
41.448
1.00
98.73
H


ATOM
2285
NE2
GLN
82
127.665
25.572
39.220
1.00
94.58
H


ATOM
2286
C
GLN
82
127.075
29.065
41.530
1.00
44.59
H


ATOM
2287
O
GLN
82
126.366
29.787
40.833
1.00
42.20
H


ATOM
2288
N
LEU
83
128.394
29.014
41.422
1.00
49.17
H


ATOM
2289
CA
LEU
83
129.114
29.797
40.434
1.00
46.27
H


ATOM
2290
CB
LEU
83
130.369
30.398
41.067
1.00
32.33
H


ATOM
2291
CG
LEU
83
130.033
31.416
42.160
1.00
31.17
H


ATOM
2292
CD1
LEU
83
131.119
31.455
43.252
1.00
6.90
H


ATOM
2293
CD2
LEU
83
129.865
32.766
41.496
1.00
18.19
H


ATOM
2294
C
LEU
83
129.471
28.845
39.298
1.00
45.11
H


ATOM
2295
O
LEU
83
130.201
27.870
39.496
1.00
36.84
H


ATOM
2296
N
SER
84
128.925
29.118
38.117
1.00
44.08
H


ATOM
2297
CA
SER
84
129.160
28.280
36.948
1.00
46.97
H


ATOM
2298
CB
SER
84
127.829
27.869
36.323
1.00
54.37
H


ATOM
2299
OG
SER
84
127.604
28.576
35.118
1.00
64.19
H


ATOM
2300
C
SER
84
130.037
28.942
35.896
1.00
49.55
H


ATOM
2301
O
SER
84
129.905
30.137
35.617
1.00
47.92
H


ATOM
2302
N
ARG
85
130.925
28.146
35.306
1.00
48.23
H


ATOM
2303
CA
ARG
85
131.842
28.637
34.294
1.00
41.86
H


ATOM
2304
CB
ARG
85
131.057
29.141
33.082
1.00
46.21
H


ATOM
2305
CG
ARG
85
130.474
28.012
32.236
1.00
41.59
H


ATOM
2306
CD
ARG
85
129.125
28.393
31.630
1.00
68.47
H


ATOM
2307
NE
ARG
85
129.174
28.406
30.170
1.00
68.50
H


ATOM
2308
CZ
ARG
85
128.142
28.126
29.381
1.00
60.66
H


ATOM
2309
NH1
ARG
85
126.962
27.808
29.906
1.00
56.39
H


ATOM
2310
NH2
ARG
85
128.293
28.166
28.065
1.00
55.47
H


ATOM
2311
C
ARG
85
132.670
29.752
34.912
1.00
40.27
H


ATOM
2312
O
ARG
85
132.430
30.939
34.669
1.00
37.17
H


ATOM
2313
N
MET
86
133.645
29.350
35.722
1.00
35.41
H


ATOM
2314
CA
MET
86
134.518
30.288
36.412
1.00
32.72
H


ATOM
2315
CB
MET
86
134.974
29.684
37.733
1.00
38.34
H


ATOM
2316
CG
MET
86
134.091
28.551
38.215
1.00
45.32
H


ATOM
2317
SD
MET
86
133.310
28.856
39.804
1.00
63.97
H


ATOM
2318
CE
MET
86
133.680
30.599
40.068
1.00
45.50
H


ATOM
2319
C
MET
86
135.733
30.691
35.592
1.00
33.88
H


ATOM
2320
O
MET
86
136.097
30.018
34.627
1.00
28.01
H


ATOM
2321
N
THR
87
136.357
31.794
35.996
1.00
37.82
H


ATOM
2322
CA
THR
87
137.525
32.330
35.310
1.00
39.63
H


ATOM
2323
CB
THR
87
137.081
33.070
34.035
1.00
31.11
H


ATOM
2324
OG1
THR
87
136.455
32.132
33.153
1.00
44.08
H


ATOM
2325
CG2
THR
87
138.263
33.706
33.324
1.00
41.18
H


ATOM
2326
C
THR
87
138.299
33.282
36.232
1.00
49.08
H


ATOM
2327
O
THR
87
137.797
33.684
37.278
1.00
56.80
H


ATOM
2328
N
SER
88
139.523
33.634
35.845
1.00
59.16
H


ATOM
2329
CA
SER
88
140.362
34.539
36.633
1.00
63.45
H


ATOM
2330
CB
SER
88
141.693
34.780
35.911
1.00
64.98
H


ATOM
2331
OG
SER
88
142.614
33.729
36.165
1.00
44.05
H


ATOM
2332
C
SER
88
139.672
35.878
36.897
1.00
61.47
H


ATOM
2333
O
SER
88
140.291
36.838
37.356
1.00
63.13
H


ATOM
2334
N
GLU
89
138.384
35.928
36.586
1.00
61.96
H


ATOM
2335
CA
GLU
89
137.567
37.114
36.791
1.00
60.40
H


ATOM
2336
CB
GLU
89
136.888
37.517
35.483
1.00
58.39
H


ATOM
2337
CG
GLU
89
136.045
36.410
34.861
1.00
65.30
H


ATOM
2338
CD
GLU
89
134.607
36.832
34.614
1.00
74.02
H


ATOM
2339
OE1
GLU
89
134.227
36.969
33.430
1.00
59.34
H


ATOM
2340
OE2
GLU
89
133.858
37.028
35.604
1.00
71.55
H


ATOM
2341
C
GLU
89
136.518
36.725
37.822
1.00
58.77
H


ATOM
2342
O
GLU
89
135.492
37.388
37.971
1.00
54.85
H


ATOM
2343
N
ASP
90
136.788
35.624
38.514
1.00
59.98
H


ATOM
2344
CA
ASP
90
135.898
35.102
39.544
1.00
62.69
H


ATOM
2345
CB
ASP
90
135.475
33.664
39.211
1.00
63.78
H


ATOM
2346
CG
ASP
90
134.268
33.601
38.293
1.00
63.07
H


ATOM
2347
OD1
ASP
90
133.728
32.488
38.109
1.00
51.98
H


ATOM
2348
OD2
ASP
90
133.864
34.653
37.757
1.00
64.25
H


ATOM
2349
C
ASP
90
136.653
35.105
40.867
1.00
58.67
H


ATOM
2350
O
ASP
90
136.052
35.098
41.939
1.00
60.42
H


ATOM
2351
N
SER
91
137.979
35.102
40.783
1.00
57.82
H


ATOM
2352
CA
SER
91
138.806
35.113
41.978
1.00
51.69
H


ATOM
2353
CB
SER
91
140.285
35.096
41.597
1.00
44.52
H


ATOM
2354
OG
SER
91
140.598
33.935
40.846
1.00
46.65
H


ATOM
2355
C
SER
91
138.487
36.362
42.787
1.00
53.31
H


ATOM
2356
O
SER
91
138.881
37.471
42.427
1.00
45.71
H


ATOM
2357
N
ALA
92
137.755
36.167
43.877
1.00
53.93
H


ATOM
2358
CA
ALA
92
137.356
37.257
44.755
1.00
50.72
H


ATOM
2359
CB
ALA
92
136.133
37.967
44.177
1.00
48.61
H


ATOM
2360
C
ALA
92
137.045
36.691
46.143
1.00
46.86
H


ATOM
2361
O
ALA
92
137.638
35.690
46.545
1.00
49.03
H


ATOM
2362
N
VAL
93
136.116
37.313
46.868
1.00
46.02
H


ATOM
2363
CA
VAL
93
135.775
36.842
48.207
1.00
44.17
H


ATOM
2364
CB
VAL
93
136.116
37.909
49.272
1.00
39.83
H


ATOM
2365
CG1
VAL
93
136.006
37.299
50.663
1.00
31.85
H


ATOM
2366
CG2
VAL
93
137.523
38.450
49.049
1.00
37.20
H


ATOM
2367
C
VAL
93
134.330
36.371
48.439
1.00
45.91
H


ATOM
2368
O
VAL
93
134.092
35.525
49.301
1.00
48.37
H


ATOM
2369
N
TYR
94
133.373
36.916
47.691
1.00
38.60
H


ATOM
2370
CA
TYR
94
131.963
36.524
47.825
1.00
49.33
H


ATOM
2371
CB
TYR
94
131.706
35.189
47.125
1.00
40.10
H


ATOM
2372
CG
TYR
94
132.160
35.137
45.692
1.00
31.43
H


ATOM
2373
CD1
TYR
94
131.372
35.660
44.675
1.00
29.40
H


ATOM
2374
CE1
TYR
94
131.758
35.556
43.344
1.00
41.76
H


ATOM
2375
CD2
TYR
94
133.355
34.512
45.346
1.00
33.02
H


ATOM
2376
CE2
TYR
94
133.750
34.402
44.025
1.00
31.73
H


ATOM
2377
CZ
TYR
94
132.949
34.922
43.028
1.00
46.94
H


ATOM
2378
OH
TYR
94
133.336
34.796
41.711
1.00
62.64
H


ATOM
2379
C
TYR
94
131.450
36.407
49.267
1.00
50.08
H


ATOM
2380
O
TYR
94
131.708
35.413
49.953
1.00
34.70
H


ATOM
2381
N
PHE
95
130.688
37.408
49.703
1.00
50.03
H


ATOM
2382
CA
PHE
95
130.140
37.431
51.051
1.00
34.71
H


ATOM
2383
CB
PHE
95
130.292
38.814
51.671
1.00
32.84
H


ATOM
2384
CG
PHE
95
131.646
39.077
52.243
1.00
19.95
H


ATOM
2385
CD1
PHE
95
131.966
38.638
53.516
1.00
6.35
H


ATOM
2386
CD2
PHE
95
132.600
39.780
51.508
1.00
14.02
H


ATOM
2387
CE1
PHE
95
133.219
38.892
54.057
1.00
29.11
H


ATOM
2388
CE2
PHE
95
133.849
40.037
52.041
1.00
21.81
H


ATOM
2389
CZ
PHE
95
134.162
39.592
53.321
1.00
20.98
H


ATOM
2390
C
PHE
95
128.678
37.082
51.077
1.00
37.58
H


ATOM
2391
O
PHE
95
127.941
37.378
50.146
1.00
40.29
H


ATOM
2392
N
CYS
96
128.272
36.455
52.168
1.00
47.35
H


ATOM
2393
CA
CYS
96
126.888
36.080
52.375
1.00
48.56
H


ATOM
2394
C
CYS
96
126.368
37.062
53.415
1.00
47.93
H


ATOM
2395
O
CYS
96
127.035
37.326
54.420
1.00
49.37
H


ATOM
2396
CB
CYS
96
126.796
34.652
52.911
1.00
45.82
H


ATOM
2397
SG
CYS
96
125.140
34.190
53.511
1.00
75.54
H


ATOM
2398
N
THR
97
125.188
37.614
53.168
1.00
37.05
H


ATOM
2399
CA
THR
97
124.595
38.575
54.086
1.00
35.84
H


ATOM
2400
CB
THR
97
125.127
40.008
53.807
1.00
33.55
H


ATOM
2401
OG1
THR
97
124.832
40.851
54.922
1.00
24.79
H


ATOM
2402
CG2
THR
97
124.517
40.584
52.542
1.00
23.01
H


ATOM
2403
C
THR
97
123.085
38.518
53.931
1.00
34.60
H


ATOM
2404
O
THR
97
122.584
37.860
53.026
1.00
34.26
H


ATOM
2405
N
ARG
98
122.356
39.192
54.814
1.00
46.98
H


ATOM
2406
CA
ARG
98
120.896
39.158
54.752
1.00
49.01
H


ATOM
2407
CB
ARG
98
120.352
38.157
55.795
1.00
38.44
H


ATOM
2408
CG
ARG
98
119.596
38.781
56.963
1.00
40.81
H


ATOM
2409
CD
ARG
98
120.319
38.594
58.296
1.00
27.38
H


ATOM
2410
NE
ARG
98
119.849
39.546
59.307
1.00
53.08
H


ATOM
2411
CZ
ARG
98
119.540
39.230
60.563
1.00
40.53
H


ATOM
2412
NH1
ARG
98
119.651
37.977
60.980
1.00
58.43
H


ATOM
2413
NH2
ARG
98
119.113
40.167
61.403
1.00
33.33
H


ATOM
2414
C
ARG
98
120.229
40.507
54.938
1.00
39.44
H


ATOM
2415
O
ARG
98
120.740
41.377
55.637
1.00
41.38
H


ATOM
2416
N
SER
99
119.079
40.666
54.296
1.00
35.04
H


ATOM
2417
CA
SER
99
118.299
41.889
54.388
1.00
24.81
H


ATOM
2418
CB
SER
99
117.920
42.396
53.004
1.00
14.04
H


ATOM
2419
OG
SER
99
117.671
43.780
53.039
1.00
29.22
H


ATOM
2420
C
SER
99
117.050
41.455
55.106
1.00
28.87
H


ATOM
2421
O
SER
99
116.660
40.294
55.009
1.00
36.12
H


ATOM
2422
N
HIS
100
116.418
42.367
55.829
1.00
34.54
H


ATOM
2423
CA
HIS
100
115.209
41.998
56.541
1.00
40.60
H


ATOM
2424
CB
HIS
100
114.976
42.943
57.714
1.00
35.00
H


ATOM
2425
CG
HIS
100
113.731
42.641
58.492
1.00
48.41
H


ATOM
2426
CD2
HIS
100
113.141
41.463
58.812
1.00
45.56
H


ATOM
2427
ND1
HIS
100
112.947
43.625
59.057
1.00
32.49
H


ATOM
2428
CE1
HIS
100
111.930
43.069
59.687
1.00
29.00
H


ATOM
2429
NE2
HIS
100
112.023
41.759
59.557
1.00
33.71
H


ATOM
2430
C
HIS
100
114.037
42.072
55.584
1.00
41.32
H


ATOM
2431
O
HIS
100
113.824
43.110
54.964
1.00
48.72
H


ATOM
2432
N
TYR
101
113.295
40.976
55.438
1.00
35.01
H


ATOM
2433
CA
TYR
101
112.136
41.001
54.551
1.00
39.86
H


ATOM
2434
CB
TYR
101
111.509
39.606
54.408
1.00
26.52
H


ATOM
2435
CG
TYR
101
110.507
39.496
53.267
1.00
26.05
H


ATOM
2436
CD1
TYR
101
110.933
39.404
51.945
1.00
40.86
H


ATOM
2437
CE1
TYR
101
110.024
39.324
50.885
1.00
27.58
H


ATOM
2438
CD2
TYR
101
109.135
39.502
53.507
1.00
33.96
H


ATOM
2439
CE2
TYR
101
108.216
39.422
52.450
1.00
40.43
H


ATOM
2440
CZ
TYR
101
108.673
39.336
51.145
1.00
27.74
H


ATOM
2441
OH
TYR
101
107.779
39.275
50.100
1.00
40.21
H


ATOM
2442
C
TYR
101
111.167
41.950
55.247
1.00
55.50
H


ATOM
2443
O
TYR
101
111.013
41.892
56.470
1.00
73.70
H


ATOM
2444
N
GLY
102
110.525
42.828
54.482
1.00
45.70
H


ATOM
2445
CA
GLY
102
109.611
43.790
55.079
1.00
43.01
H


ATOM
2446
C
GLY
102
110.364
45.094
55.237
1.00
39.11
H


ATOM
2447
O
GLY
102
110.169
46.028
54.464
1.00
47.90
H


ATOM
2448
N
LEU
103
111.229
45.155
56.243
1.00
31.80
H


ATOM
2449
CA
LEU
103
112.062
46.331
56.475
1.00
34.98
H


ATOM
2450
CB
LEU
103
112.270
46.554
57.975
1.00
47.24
H


ATOM
2451
CG
LEU
103
111.258
47.406
58.750
1.00
56.12
H


ATOM
2452
CD1
LEU
103
109.959
47.593
57.956
1.00
31.67
H


ATOM
2453
CD2
LEU
103
110.993
46.730
60.092
1.00
44.52
H


ATOM
2454
C
LEU
103
113.394
46.019
55.807
1.00
31.85
H


ATOM
2455
O
LEU
103
114.392
45.736
56.471
1.00
32.76
H


ATOM
2456
N
ASP
104
113.368
46.045
54.477
1.00
33.42
H


ATOM
2457
CA
ASP
104
114.512
45.757
53.625
1.00
20.79
H


ATOM
2458
CB
ASP
104
113.992
45.202
52.295
1.00
32.02
H


ATOM
2459
CG
ASP
104
115.093
44.901
51.298
1.00
55.62
H


ATOM
2460
OD1
ASP
104
114.753
44.583
50.136
1.00
38.63
H


ATOM
2461
OD2
ASP
104
116.286
44.985
51.663
1.00
61.09
H


ATOM
2462
C
ASP
104
115.255
47.067
53.415
1.00
29.02
H


ATOM
2463
O
ASP
104
115.021
47.775
52.440
1.00
33.69
H


ATOM
2464
N
TRP
105
116.148
47.386
54.346
1.00
37.55
H


ATOM
2465
CA
TRP
105
116.907
48.628
54.288
1.00
32.28
H


ATOM
2466
CB
TRP
105
116.599
49.484
55.518
1.00
42.44
H


ATOM
2467
CG
TRP
105
115.123
49.751
55.717
1.00
44.69
H


ATOM
2468
CD2
TRP
105
114.456
50.028
56.955
1.00
43.59
H


ATOM
2469
CE2
TRP
105
113.091
50.217
56.662
1.00
39.38
H


ATOM
2470
CE3
TRP
105
114.887
50.136
58.285
1.00
53.16
H


ATOM
2471
CD1
TRP
105
114.157
49.780
54.751
1.00
25.93
H


ATOM
2472
NE1
TRP
105
112.942
50.059
55.313
1.00
37.01
H


ATOM
2473
CZ2
TRP
105
112.144
50.507
57.650
1.00
45.54
H


ATOM
2474
CZ3
TRP
105
113.945
50.425
59.270
1.00
48.69
H


ATOM
2475
CH2
TRP
105
112.588
50.609
58.945
1.00
55.24
H


ATOM
2476
C
TRP
105
118.405
48.421
54.187
1.00
40.80
H


ATOM
2477
O
TRP
105
119.063
49.042
53.352
1.00
47.85
H


ATOM
2478
N
ASN
106
118.952
47.556
55.038
1.00
49.48
H


ATOM
2479
CA
ASN
106
120.389
47.305
55.013
1.00
46.39
H


ATOM
2480
CB
ASN
106
121.103
48.280
55.951
1.00
36.85
H


ATOM
2481
CG
ASN
106
120.869
47.961
57.408
1.00
28.91
H


ATOM
2482
OD1
ASN
106
119.909
48.434
58.013
1.00
32.97
H


ATOM
2483
ND2
ASN
106
121.744
47.152
57.981
1.00
41.71
H


ATOM
2484
C
ASN
106
120.786
45.877
55.363
1.00
48.25
H


ATOM
2485
O
ASN
106
119.950
45.059
55.760
1.00
46.92
H


ATOM
2486
N
PHE
107
122.079
45.602
55.202
1.00
47.37
H


ATOM
2487
CA
PHE
107
122.665
44.294
55.489
1.00
47.06
H


ATOM
2488
CB
PHE
107
123.589
43.862
54.341
1.00
51.22
H


ATOM
2489
CG
PHE
107
123.004
44.052
52.954
1.00
36.58
H


ATOM
2490
CD1
PHE
107
121.628
44.053
52.737
1.00
43.99
H


ATOM
2491
CD2
PHE
107
123.847
44.180
51.851
1.00
35.28
H


ATOM
2492
CE1
PHE
107
121.107
44.175
51.449
1.00
41.51
H


ATOM
2493
CE2
PHE
107
123.334
44.301
50.561
1.00
20.31
H


ATOM
2494
CZ
PHE
107
121.965
44.296
50.362
1.00
40.17
H


ATOM
2495
C
PHE
107
123.479
44.386
56.787
1.00
45.87
H


ATOM
2496
O
PHE
107
124.641
44.805
56.765
1.00
38.80
H


ATOM
2497
N
ASP
108
122.874
43.982
57.905
1.00
44.31
H


ATOM
2498
CA
ASP
108
123.528
44.053
59.215
1.00
40.48
H


ATOM
2499
CB
ASP
108
122.479
44.243
60.325
1.00
54.96
H


ATOM
2500
CG
ASP
108
121.048
44.057
59.833
1.00
66.26
H


ATOM
2501
OD1
ASP
108
120.304
45.059
59.790
1.00
56.03
H


ATOM
2502
OD2
ASP
108
120.664
42.914
59.494
1.00
63.18
H


ATOM
2503
C
ASP
108
124.445
42.889
59.600
1.00
27.53
H


ATOM
2504
O
ASP
108
125.418
43.077
60.328
1.00
16.20
H


ATOM
2505
N
VAL
109
124.133
41.690
59.128
1.00
18.58
H


ATOM
2506
CA
VAL
109
124.946
40.527
59.458
1.00
20.11
H


ATOM
2507
CB
VAL
109
124.072
39.410
60.037
1.00
25.57
H


ATOM
2508
CG1
VAL
109
124.936
38.353
60.691
1.00
22.40
H


ATOM
2509
CG2
VAL
109
123.098
39.998
61.038
1.00
21.93
H


ATOM
2510
C
VAL
109
125.704
39.984
58.246
1.00
26.64
H


ATOM
2511
O
VAL
109
125.110
39.594
57.248
1.00
34.79
H


ATOM
2512
N
TRP
110
127.023
39.949
58.336
1.00
27.30
H


ATOM
2513
CA
TRP
110
127.818
39.459
57.229
1.00
27.28
H


ATOM
2514
CB
TRP
110
128.834
40.519
56.810
1.00
33.78
H


ATOM
2515
CG
TRP
110
128.248
41.738
56.167
1.00
36.21
H


ATOM
2516
CD2
TRP
110
128.440
42.162
54.813
1.00
25.48
H


ATOM
2517
CE2
TRP
110
127.801
43.411
54.669
1.00
33.24
H


ATOM
2518
CE3
TRP
110
129.097
41.609
53.711
1.00
13.14
H


ATOM
2519
CD1
TRP
110
127.511
42.715
56.770
1.00
42.55
H


ATOM
2520
NE1
TRP
110
127.236
43.728
55.879
1.00
36.86
H


ATOM
2521
CZ2
TRP
110
127.795
44.115
53.467
1.00
25.21
H


ATOM
2522
CZ3
TRP
110
129.096
42.314
52.511
1.00
30.00
H


ATOM
2523
CH2
TRP
110
128.449
43.553
52.402
1.00
26.50
H


ATOM
2524
C
TRP
110
128.566
38.187
57.601
1.00
29.69
H


ATOM
2525
O
TRP
110
128.946
37.993
58.753
1.00
39.20
H


ATOM
2526
N
GLY
111
128.774
37.317
56.621
1.00
26.69
H


ATOM
2527
CA
GLY
111
129.523
36.110
56.880
1.00
15.66
H


ATOM
2528
C
GLY
111
130.988
36.505
56.847
1.00
32.31
H


ATOM
2529
O
GLY
111
131.327
37.622
56.451
1.00
24.60
H


ATOM
2530
N
ALA
112
131.867
35.604
57.261
1.00
41.72
H


ATOM
2531
CA
ALA
112
133.287
35.907
57.249
1.00
41.34
H


ATOM
2532
CB
ALA
112
134.033
34.911
58.119
1.00
39.24
H


ATOM
2533
C
ALA
112
133.857
35.896
55.826
1.00
45.93
H


ATOM
2534
O
ALA
112
134.902
36.492
55.568
1.00
49.85
H


ATOM
2535
N
GLY
113
133.178
35.217
54.906
1.00
45.09
H


ATOM
2536
CA
GLY
113
133.665
35.161
53.538
1.00
44.98
H


ATOM
2537
C
GLY
113
134.180
33.798
53.099
1.00
44.48
H


ATOM
2538
O
GLY
113
134.273
32.862
53.894
1.00
40.99
H


ATOM
2539
N
THR
114
134.519
33.690
51.819
1.00
44.31
H


ATOM
2540
CA
THR
114
135.019
32.446
51.242
1.00
31.88
H


ATOM
2541
CB
THR
114
133.845
31.530
50.772
1.00
20.38
H


ATOM
2542
OG1
THR
114
133.279
30.861
51.900
1.00
35.87
H


ATOM
2543
CG2
THR
114
134.328
30.478
49.789
1.00
32.39
H


ATOM
2544
C
THR
114
135.865
32.814
50.032
1.00
33.60
H


ATOM
2545
O
THR
114
135.325
33.054
48.953
1.00
42.98
H


ATOM
2546
N
THR
115
137.182
32.880
50.208
1.00
35.92
H


ATOM
2547
CA
THR
115
138.055
33.208
49.089
1.00
33.21
H


ATOM
2548
CB
THR
115
139.525
33.317
49.499
1.00
43.78
H


ATOM
2549
OG1
THR
115
139.649
34.185
50.632
1.00
41.45
H


ATOM
2550
CG2
THR
115
140.351
33.867
48.332
1.00
43.50
H


ATOM
2551
C
THR
115
137.938
32.100
48.059
1.00
39.18
H


ATOM
2552
O
THR
115
137.823
30.918
48.406
1.00
28.11
H


ATOM
2553
N
VAL
116
137.958
32.490
46.791
1.00
36.51
H


ATOM
2554
CA
VAL
116
137.840
31.537
45.708
1.00
40.64
H


ATOM
2555
CB
VAL
116
136.443
31.616
45.051
1.00
41.04
H


ATOM
2556
CG1
VAL
116
136.337
30.599
43.931
1.00
35.25
H


ATOM
2557
CG2
VAL
116
135.362
31.359
46.096
1.00
33.28
H


ATOM
2558
C
VAL
116
138.899
31.816
44.661
1.00
43.76
H


ATOM
2559
O
VAL
116
139.009
32.929
44.146
1.00
46.23
H


ATOM
2560
N
THR
117
139.683
30.791
44.354
1.00
44.39
H


ATOM
2561
CA
THR
117
140.740
30.919
43.370
1.00
40.89
H


ATOM
2562
CB
THR
117
142.095
30.552
43.969
1.00
38.40
H


ATOM
2563
OG1
THR
117
142.320
31.340
45.145
1.00
36.59
H


ATOM
2564
CG2
THR
117
143.205
30.810
42.966
1.00
32.70
H


ATOM
2565
C
THR
117
140.449
30.001
42.203
1.00
38.02
H


ATOM
2566
O
THR
117
139.802
28.971
42.357
1.00
37.58
H


ATOM
2567
N
VAL
118
140.928
30.386
41.031
1.00
45.45
H


ATOM
2568
CA
VAL
118
140.705
29.597
39.838
1.00
47.20
H


ATOM
2569
CB
VAL
118
140.199
30.481
38.694
1.00
54.07
H


ATOM
2570
CG1
VAL
118
138.698
30.704
38.844
1.00
40.64
H


ATOM
2571
CG2
VAL
118
140.942
31.807
38.700
1.00
46.30
H


ATOM
2572
C
VAL
118
141.973
28.890
39.400
1.00
42.28
H


ATOM
2573
O
VAL
118
141.946
27.706
39.060
1.00
42.04
H


ATOM
2574
N
SER
119
143.081
29.622
39.415
1.00
40.12
H


ATOM
2575
CA
SER
119
144.381
29.089
39.015
1.00
48.99
H


ATOM
2576
CB
SER
119
145.469
29.569
39.983
1.00
49.51
H


ATOM
2577
OG
SER
119
146.419
30.398
39.331
1.00
31.69
H


ATOM
2578
C
SER
119
144.418
27.563
38.929
1.00
49.87
H


ATOM
2579
O
SER
119
144.028
26.856
39.870
1.00
42.52
H


ATOM
2580
N
SER
120
144.887
27.067
37.787
1.00
55.96
H


ATOM
2581
CA
SER
120
144.996
25.633
37.540
1.00
53.80
H


ATOM
2582
CB
SER
120
144.774
25.334
36.050
1.00
57.59
H


ATOM
2583
OG
SER
120
145.785
25.919
35.239
1.00
55.34
H


ATOM
2584
C
SER
120
146.377
25.145
37.965
1.00
53.97
H


ATOM
2585
O
SER
120
146.879
24.149
37.447
1.00
49.63
H


ATOM
2586
N
ALA
121
146.974
25.858
38.919
1.00
62.49
H


ATOM
2587
CA
ALA
121
148.307
25.544
39.445
1.00
58.34
H


ATOM
2588
CB
ALA
121
148.830
26.714
40.278
1.00
52.59
H


ATOM
2589
C
ALA
121
148.333
24.268
40.284
1.00
54.04
H


ATOM
2590
O
ALA
121
147.318
23.584
40.432
1.00
56.38
H


ATOM
2591
N
LYS
122
149.498
23.951
40.841
1.00
48.35
H


ATOM
2592
CA
LYS
122
149.624
22.750
41.649
1.00
46.60
H


ATOM
2593
CB
LYS
122
150.912
21.998
41.318
1.00
51.72
H


ATOM
2594
CG
LYS
122
150.682
20.565
40.843
1.00
51.16
H


ATOM
2595
CD
LYS
122
150.117
19.675
41.948
1.00
40.43
H


ATOM
2596
CE
LYS
122
151.191
18.754
42.525
1.00
52.69
H


ATOM
2597
NZ
LYS
122
150.980
17.320
42.166
1.00
52.23
H


ATOM
2598
C
LYS
122
149.577
23.043
43.133
1.00
52.24
H


ATOM
2599
O
LYS
122
150.258
23.933
43.644
1.00
51.54
H


ATOM
2600
N
THR
123
148.758
22.255
43.811
1.00
58.75
H


ATOM
2601
CA
THR
123
148.562
22.363
45.249
1.00
59.16
H


ATOM
2602
CB
THR
123
147.422
21.459
45.722
1.00
70.62
H


ATOM
2603
OG1
THR
123
146.234
21.774
44.989
1.00
100.00
H


ATOM
2604
CG2
THR
123
147.174
21.644
47.216
1.00
71.56
H


ATOM
2605
C
THR
123
149.851
21.904
45.913
1.00
59.76
H


ATOM
2606
O
THR
123
150.327
20.797
45.654
1.00
51.50
H


ATOM
2607
N
THR
124
150.430
22.671
46.957
1.00
60.58
H


ATOM
2608
CA
THR
124
151.805
22.409
47.339
1.00
54.63
H


ATOM
2609
CB
THR
124
152.776
23.187
46.433
1.00
44.65
H


ATOM
2610
OG1
THR
124
152.458
22.930
45.055
1.00
43.48
H


ATOM
2611
CG2
THR
124
154.207
22.779
46.719
1.00
35.75
H


ATOM
2612
C
THR
124
152.034
22.844
48.776
1.00
61.90
H


ATOM
2613
O
THR
124
151.832
24.005
49.113
1.00
66.47
H


ATOM
2614
N
PRO
125
152.470
21.918
49.644
1.00
63.12
H


ATOM
2615
CD
PRO
125
152.792
20.505
49.387
1.00
43.60
H


ATOM
2616
CA
PRO
125
152.705
22.286
51.044
1.00
64.11
H


ATOM
2617
CB
PRO
125
153.012
20.956
51.725
1.00
53.31
H


ATOM
2618
CG
PRO
125
153.525
20.089
50.636
1.00
27.09
H


ATOM
2619
C
PRO
125
153.858
23.274
51.141
1.00
60.12
H


ATOM
2620
O
PRO
125
154.781
23.255
50.329
1.00
49.68
H


ATOM
2621
N
PRO
126
153.811
24.153
52.140
1.00
55.19
H


ATOM
2622
CD
PRO
126
152.761
24.258
53.169
1.00
61.07
H


ATOM
2623
CA
PRO
126
154.855
25.156
52.333
1.00
47.53
H


ATOM
2624
CB
PRO
126
154.213
26.141
53.296
1.00
49.84
H


ATOM
2625
CG
PRO
126
153.315
25.289
54.118
1.00
73.92
H


ATOM
2626
C
PRO
126
156.155
24.608
52.891
1.00
49.11
H


ATOM
2627
O
PRO
126
156.148
23.695
53.716
1.00
53.71
H


ATOM
2628
N
SER
127
157.266
25.174
52.429
1.00
38.85
H


ATOM
2629
CA
SER
127
158.586
24.792
52.913
1.00
21.97
H


ATOM
2630
CB
SER
127
159.628
24.904
51.798
1.00
39.50
H


ATOM
2631
OG
SER
127
159.487
23.866
50.845
1.00
52.42
H


ATOM
2632
C
SER
127
158.864
25.826
53.990
1.00
33.98
H


ATOM
2633
O
SER
127
159.021
27.008
53.687
1.00
34.88
H


ATOM
2634
N
VAL
128
158.903
25.393
55.243
1.00
39.20
H


ATOM
2635
CA
VAL
128
159.135
26.308
56.353
1.00
46.26
H


ATOM
2636
CB
VAL
128
158.247
25.929
57.562
1.00
49.37
H


ATOM
2637
CG1
VAL
128
158.162
27.089
58.545
1.00
42.90
H


ATOM
2638
CG2
VAL
128
156.862
25.538
57.078
1.00
51.53
H


ATOM
2639
C
VAL
128
160.594
26.350
56.794
1.00
37.55
H


ATOM
2640
O
VAL
128
161.183
25.329
57.153
1.00
49.56
H


ATOM
2641
N
TYR
129
161.172
27.545
56.765
1.00
48.51
H


ATOM
2642
CA
TYR
129
162.562
27.740
57.150
1.00
44.86
H


ATOM
2643
CB
TYR
129
163.364
28.279
55.969
1.00
35.54
H


ATOM
2644
CG
TYR
129
163.348
27.402
54.736
1.00
27.55
H


ATOM
2645
CD1
TYR
129
163.718
26.059
54.800
1.00
42.44
H


ATOM
2646
CE1
TYR
129
163.729
25.256
53.654
1.00
44.45
H


ATOM
2647
CD2
TYR
129
162.986
27.928
53.497
1.00
28.77
H


ATOM
2648
CE2
TYR
129
162.991
27.135
52.345
1.00
44.26
H


ATOM
2649
CZ
TYR
129
163.363
25.806
52.430
1.00
46.95
H


ATOM
2650
OH
TYR
129
163.366
25.036
51.291
1.00
71.81
H


ATOM
2651
C
TYR
129
162.670
28.722
58.312
1.00
41.49
H


ATOM
2652
O
TYR
129
162.195
29.856
58.225
1.00
48.76
H


ATOM
2653
N
PRO
130
163.309
28.302
59.415
1.00
46.24
H


ATOM
2654
CD
PRO
130
163.913
26.972
59.604
1.00
58.08
H


ATOM
2655
CA
PRO
130
163.482
29.152
60.597
1.00
40.62
H


ATOM
2656
CB
PRO
130
163.970
28.180
61.661
1.00
49.21
H


ATOM
2657
CG
PRO
130
164.699
27.132
60.872
1.00
50.86
H


ATOM
2658
C
PRO
130
164.486
30.276
60.359
1.00
42.13
H


ATOM
2659
O
PRO
130
165.506
30.079
59.704
1.00
49.80
H


ATOM
2660
N
LEU
131
164.195
31.455
60.895
1.00
40.92
H


ATOM
2661
CA
LEU
131
165.081
32.598
60.732
1.00
35.23
H


ATOM
2662
CB
LEU
131
164.319
33.772
60.120
1.00
34.49
H


ATOM
2663
CG
LEU
131
163.916
33.644
58.652
1.00
34.92
H


ATOM
2664
CD1
LEU
131
163.135
34.878
58.245
1.00
11.51
H


ATOM
2665
CD2
LEU
131
165.144
33.483
57.781
1.00
26.67
H


ATOM
2666
C
LEU
131
165.666
33.014
62.077
1.00
40.36
H


ATOM
2667
O
LEU
131
164.956
33.557
62.927
1.00
46.95
H


ATOM
2668
N
ALA
132
166.956
32.755
62.263
1.00
56.70
H


ATOM
2669
CA
ALA
132
167.632
33.110
63.507
1.00
56.71
H


ATOM
2670
CB
ALA
132
168.300
31.886
64.096
1.00
57.53
H


ATOM
2671
C
ALA
132
168.662
34.210
63.268
1.00
55.87
H


ATOM
2672
O
ALA
132
169.276
34.279
62.204
1.00
54.54
H


ATOM
2673
N
PRO
133
168.864
35.091
64.262
1.00
57.72
H


ATOM
2674
CD
PRO
133
168.161
35.065
65.557
1.00
47.07
H


ATOM
2675
CA
PRO
133
169.815
36.206
64.187
1.00
60.50
H


ATOM
2676
CB
PRO
133
169.496
37.030
65.431
1.00
59.11
H


ATOM
2677
CG
PRO
133
168.946
36.033
66.393
1.00
46.85
H


ATOM
2678
C
PRO
133
171.285
35.780
64.154
1.00
64.47
H


ATOM
2679
O
PRO
133
171.743
35.030
65.021
1.00
63.82
H


ATOM
2680
N
GLY
134
172.021
36.276
63.161
1.00
66.38
H


ATOM
2681
CA
GLY
134
173.429
35.937
63.030
1.00
54.50
H


ATOM
2682
C
GLY
134
174.356
36.531
64.074
1.00
60.13
H


ATOM
2683
O
GLY
134
175.515
36.851
63.795
1.00
70.75
H


ATOM
2684
N
SER
135
173.893
36.652
65.276
1.00
74.93
H


ATOM
2685
CA
SER
135
174.743
37.216
66.319
1.00
86.16
H


ATOM
2686
CB
SER
135
176.038
36.396
66.439
1.00
90.22
H


ATOM
2687
OG
SER
135
176.965
36.773
65.426
1.00
100.00
H


ATOM
2688
C
SER
135
175.096
38.660
65.950
1.00
88.57
H


ATOM
2689
O
SER
135
176.088
38.928
65.255
1.00
77.68
H


ATOM
2690
N
ALA
136
174.255
39.552
66.431
1.00
94.60
H


ATOM
2691
CA
ALA
136
174.413
40.992
66.206
1.00
96.15
H


ATOM
2692
CB
ALA
136
174.299
41.304
64.714
1.00
91.75
H


ATOM
2693
C
ALA
136
173.334
41.759
66.962
1.00
99.99
H


ATOM
2694
O
ALA
136
172.158
41.398
66.918
1.00
99.98
H


ATOM
2695
N
ALA
137
173.740
42.818
67.655
1.00
99.99
H


ATOM
2696
CA
ALA
137
172.802
43.637
68.417
1.00
100.00
H


ATOM
2697
CB
ALA
137
173.565
44.631
69.285
1.00
89.73
H


ATOM
2698
C
ALA
137
171.860
44.377
67.474
1.00
99.97
H


ATOM
2699
O
ALA
137
172.119
45.518
67.094
1.00
99.95
H


ATOM
2700
N
GLN
138
170.768
43.718
67.103
1.00
99.99
H


ATOM
2701
CA
GLN
138
169.788
44.308
66.198
1.00
99.99
H


ATOM
2702
CB
GLN
138
168.740
43.264
65.804
1.00
92.32
H


ATOM
2703
CG
GLN
138
167.946
42.702
66.972
1.00
80.27
H


ATOM
2704
CD
GLN
138
166.461
42.627
66.679
1.00
62.10
H


ATOM
2705
OE1
GLN
138
166.051
42.463
65.530
1.00
72.23
H


ATOM
2706
NE2
GLN
138
165.646
42.749
67.720
1.00
47.02
H


ATOM
2707
C
GLN
138
169.100
45.522
66.818
1.00
99.97
H


ATOM
2708
O
GLN
138
168.850
46.516
66.136
1.00
99.97
H


ATOM
2709
N
THR
139
168.797
45.438
68.116
1.00
99.99
H


ATOM
2710
CA
THR
139
168.138
46.530
68.810
1.00
100.00
H


ATOM
2711
CB
THR
139
166.629
46.520
68.570
1.00
100.00
H


ATOM
2712
OG1
THR
139
166.068
45.306
69.085
1.00
99.99
H


ATOM
2713
CG2
THR
139
166.326
46.637
67.084
1.00
99.99
H


ATOM
2714
C
THR
139
168.421
46.461
70.301
1.00
99.99
H


ATOM
2715
O
THR
139
167.865
45.622
71.014
1.00
99.98
H


ATOM
2716
N
ASN
140
169.282
47.359
70.792
1.00
99.98
H


ATOM
2717
CA
ASN
140
169.654
47.435
72.187
1.00
96.92
H


ATOM
2718
CB
ASN
140
168.714
48.381
72.927
1.00
94.82
H


ATOM
2719
CG
ASN
140
168.783
49.786
72.357
1.00
91.71
H


ATOM
2720
OD1
ASN
140
169.866
50.280
72.032
1.00
88.37
H


ATOM
2721
ND2
ASN
140
167.633
50.433
72.229
1.00
91.05
H


ATOM
2722
C
ASN
140
169.724
46.052
72.801
1.00
93.43
H


ATOM
2723
O
ASN
140
170.715
45.321
72.647
1.00
83.04
H


ATOM
2724
N
SER
141
168.684
45.691
73.529
1.00
91.74
H


ATOM
2725
CA
SER
141
168.629
44.391
74.200
1.00
78.99
H


ATOM
2726
CB
SER
141
168.101
44.544
75.629
1.00
69.11
H


ATOM
2727
OG
SER
141
166.899
45.297
75.639
1.00
60.50
H


ATOM
2728
C
SER
141
167.726
43.431
73.432
1.00
75.37
H


ATOM
2729
O
SER
141
168.173
42.413
72.896
1.00
88.08
H


ATOM
2730
N
MET
142
166.479
43.787
73.391
1.00
59.56
H


ATOM
2731
CA
MET
142
165.449
42.970
72.766
1.00
44.97
H


ATOM
2732
CB
MET
142
164.206
43.807
72.516
1.00
56.70
H


ATOM
2733
CG
MET
142
163.645
44.397
73.800
1.00
86.09
H


ATOM
2734
SD
MET
142
162.177
45.354
73.535
1.00
99.97
H


ATOM
2735
CE
MET
142
162.239
46.814
74.546
1.00
99.42
H


ATOM
2736
C
MET
142
165.871
42.372
71.432
1.00
32.94
H


ATOM
2737
O
MET
142
166.780
42.880
70.772
1.00
33.63
H


ATOM
2738
N
VAL
143
165.183
41.304
71.027
1.00
27.18
H


ATOM
2739
CA
VAL
143
165.501
40.615
69.779
1.00
24.76
H


ATOM
2740
CB
VAL
143
166.197
39.272
70.065
1.00
10.57
H


ATOM
2741
CG1
VAL
143
165.186
38.268
70.594
1.00
34.15
H


ATOM
2742
CG2
VAL
143
166.872
38.750
68.800
1.00
21.19
H


ATOM
2743
C
VAL
143
164.299
40.345
68.871
1.00
32.37
H


ATOM
2744
O
VAL
143
163.150
40.451
69.293
1.00
31.65
H


ATOM
2745
N
THR
144
164.588
39.981
67.623
1.00
32.76
H


ATOM
2746
CA
THR
144
163.561
39.686
66.630
1.00
32.18
H


ATOM
2747
CB
THR
144
163.459
40.810
65.580
1.00
23.79
H


ATOM
2748
OG1
THR
144
162.934
41.991
66.196
1.00
53.98
H


ATOM
2749
CG2
THR
144
162.543
40.394
64.436
1.00
20.69
H


ATOM
2750
C
THR
144
163.855
38.384
65.891
1.00
39.99
H


ATOM
2751
O
THR
144
164.958
38.178
65.384
1.00
46.16
H


ATOM
2752
N
LEU
145
162.858
37.507
65.830
1.00
32.72
H


ATOM
2753
CA
LEU
145
163.004
36.237
65.138
1.00
13.00
H


ATOM
2754
CB
LEU
145
162.645
35.082
66.062
1.00
22.16
H


ATOM
2755
CG
LEU
145
163.748
34.615
67.003
1.00
24.64
H


ATOM
2756
CD1
LEU
145
163.529
35.237
68.376
1.00
21.35
H


ATOM
2757
CD2
LEU
145
163.736
33.095
67.084
1.00
18.63
H


ATOM
2758
C
LEU
145
162.064
36.220
63.948
1.00
24.06
H


ATOM
2759
O
LEU
145
161.188
37.073
63.831
1.00
32.59
H


ATOM
2760
N
GLY
146
162.244
35.240
63.072
1.00
32.97
H


ATOM
2761
CA
GLY
146
161.397
35.153
61.903
1.00
32.08
H


ATOM
2762
C
GLY
146
161.254
33.741
61.381
1.00
29.89
H


ATOM
2763
O
GLY
146
161.995
32.845
61.778
1.00
19.61
H


ATOM
2764
N
CYS
147
160.283
33.553
60.499
1.00
39.08
H


ATOM
2765
CA
CYS
147
160.023
32.258
59.885
1.00
38.87
H


ATOM
2766
C
CYS
147
159.696
32.518
58.425
1.00
30.42
H


ATOM
2767
O
CYS
147
158.934
33.431
58.109
1.00
37.75
H


ATOM
2768
CB
CYS
147
158.843
31.560
60.574
1.00
55.35
H


ATOM
2769
SG
CYS
147
159.313
30.021
61.435
1.00
81.70
H


ATOM
2770
N
LEU
148
160.288
31.730
57.536
1.00
28.82
H


ATOM
2771
CA
LEU
148
160.037
31.891
56.115
1.00
27.56
H


ATOM
2772
CB
LEU
148
161.363
31.967
55.352
1.00
34.75
H


ATOM
2773
CG
LEU
148
161.254
32.129
53.831
1.00
34.95
H


ATOM
2774
CD1
LEU
148
160.549
33.425
53.488
1.00
18.93
H


ATOM
2775
CD2
LEU
148
162.636
32.111
53.219
1.00
21.69
H


ATOM
2776
C
LEU
148
159.187
30.737
55.580
1.00
24.86
H


ATOM
2777
O
LEU
148
159.576
29.572
55.663
1.00
30.70
H


ATOM
2778
N
VAL
149
158.020
31.077
55.045
1.00
27.57
H


ATOM
2779
CA
VAL
149
157.091
30.096
54.487
1.00
25.23
H


ATOM
2780
CB
VAL
149
155.658
30.376
54.969
1.00
3.47
H


ATOM
2781
CG1
VAL
149
154.800
29.127
54.827
1.00
25.66
H


ATOM
2782
CG2
VAL
149
155.695
30.830
56.417
1.00
15.58
H


ATOM
2783
C
VAL
149
157.152
30.207
52.969
1.00
43.19
H


ATOM
2784
O
VAL
149
156.520
31.080
52.375
1.00
55.54
H


ATOM
2785
N
LYS
150
157.906
29.313
52.341
1.00
45.51
H


ATOM
2786
CA
LYS
150
158.078
29.377
50.897
1.00
36.67
H


ATOM
2787
CB
LYS
150
159.572
29.376
50.570
1.00
23.27
H


ATOM
2788
CG
LYS
150
159.950
30.226
49.366
1.00
37.80
H


ATOM
2789
CD
LYS
150
161.441
30.145
49.070
1.00
45.44
H


ATOM
2790
CE
LYS
150
161.689
29.729
47.631
1.00
47.90
H


ATOM
2791
NZ
LYS
150
163.035
30.147
47.149
1.00
74.67
H


ATOM
2792
C
LYS
150
157.393
28.322
50.047
1.00
47.62
H


ATOM
2793
O
LYS
150
157.086
27.225
50.503
1.00
61.91
H


ATOM
2794
N
GLY
151
157.172
28.686
48.789
1.00
52.19
H


ATOM
2795
CA
GLY
151
156.562
27.808
47.805
1.00
56.42
H


ATOM
2796
C
GLY
151
155.344
26.981
48.176
1.00
65.95
H


ATOM
2797
O
GLY
151
155.404
25.751
48.146
1.00
83.19
H


ATOM
2798
N
TYR
152
154.238
27.641
48.522
1.00
68.59
H


ATOM
2799
CA
TYR
152
153.018
26.919
48.866
1.00
57.51
H


ATOM
2800
CB
TYR
152
152.646
27.131
50.342
1.00
55.56
H


ATOM
2801
CG
TYR
152
152.211
28.529
50.695
1.00
49.75
H


ATOM
2802
CD1
TYR
152
150.893
28.938
50.497
1.00
37.14
H


ATOM
2803
CE1
TYR
152
150.474
30.222
50.847
1.00
37.53
H


ATOM
2804
CD2
TYR
152
153.104
29.437
51.250
1.00
44.59
H


ATOM
2805
CE2
TYR
152
152.696
30.726
51.605
1.00
49.92
H


ATOM
2806
CZ
TYR
152
151.379
31.107
51.400
1.00
51.57
H


ATOM
2807
OH
TYR
152
150.967
32.371
51.745
1.00
62.33
H


ATOM
2808
C
TYR
152
151.874
27.358
47.965
1.00
48.01
H


ATOM
2809
O
TYR
152
151.962
28.386
47.291
1.00
28.34
H


ATOM
2810
N
PHE
153
150.810
26.567
47.947
1.00
50.83
H


ATOM
2811
CA
PHE
153
149.646
26.857
47.125
1.00
59.21
H


ATOM
2812
CB
PHE
153
150.003
26.728
45.646
1.00
64.09
H


ATOM
2813
CG
PHE
153
148.926
27.214
44.725
1.00
66.51
H


ATOM
2814
CD1
PHE
153
148.786
28.571
44.464
1.00
66.83
H


ATOM
2815
CD2
PHE
153
148.036
26.323
44.147
1.00
60.06
H


ATOM
2816
CE1
PHE
153
147.770
29.037
43.638
1.00
55.52
H


ATOM
2817
CE2
PHE
153
147.013
26.775
43.316
1.00
50.07
H


ATOM
2818
CZ
PHE
153
146.880
28.138
43.061
1.00
49.86
H


ATOM
2819
C
PHE
153
148.499
25.907
47.449
1.00
52.00
H


ATOM
2820
O
PHE
153
148.709
24.702
47.619
1.00
57.40
H


ATOM
2821
N
PRO
154
147.265
26.436
47.538
1.00
46.46
H


ATOM
2822
CD
PRO
154
146.057
25.644
47.815
1.00
39.60
H


ATOM
2823
CA
PRO
154
146.931
27.851
47.353
1.00
44.22
H


ATOM
2824
CB
PRO
154
145.533
27.795
46.772
1.00
48.46
H


ATOM
2825
CG
PRO
154
144.923
26.614
47.482
1.00
49.48
H


ATOM
2826
C
PRO
154
146.932
28.559
48.694
1.00
48.75
H


ATOM
2827
O
PRO
154
147.255
27.958
49.722
1.00
45.97
H


ATOM
2828
N
GLU
155
146.573
29.835
48.683
1.00
45.51
H


ATOM
2829
CA
GLU
155
146.508
30.585
49.927
1.00
46.54
H


ATOM
2830
CB
GLU
155
146.287
32.072
49.649
1.00
42.15
H


ATOM
2831
CG
GLU
155
147.565
32.888
49.519
1.00
52.55
H


ATOM
2832
CD
GLU
155
147.866
33.696
50.773
1.00
54.96
H


ATOM
2833
OE1
GLU
155
148.005
33.096
51.862
1.00
38.84
H


ATOM
2834
OE2
GLU
155
147.964
34.938
50.667
1.00
57.00
H


ATOM
2835
C
GLU
155
145.292
30.003
50.625
1.00
43.57
H


ATOM
2836
O
GLU
155
144.445
29.388
49.974
1.00
52.29
H


ATOM
2837
N
PRO
156
145.203
30.148
51.953
1.00
28.63
H


ATOM
2838
CD
PRO
156
143.990
29.751
52.682
1.00
21.85
H


ATOM
2839
CA
PRO
156
146.134
30.804
52.873
1.00
32.78
H


ATOM
2840
CB
PRO
156
145.212
31.685
53.699
1.00
41.77
H


ATOM
2841
CG
PRO
156
143.896
30.824
53.775
1.00
10.50
H


ATOM
2842
C
PRO
156
146.879
29.797
53.776
1.00
40.11
H


ATOM
2843
O
PRO
156
146.757
28.583
53.613
1.00
30.70
H


ATOM
2844
N
VAL
157
147.641
30.333
54.722
1.00
47.16
H


ATOM
2845
CA
VAL
157
148.383
29.540
55.693
1.00
58.27
H


ATOM
2846
CB
VAL
157
149.898
29.452
55.362
1.00
52.20
H


ATOM
2847
CG1
VAL
157
150.106
28.797
54.011
1.00
56.74
H


ATOM
2848
CG2
VAL
157
150.525
30.837
55.387
1.00
45.36
H


ATOM
2849
C
VAL
157
148.239
30.258
57.032
1.00
68.19
H


ATOM
2850
O
VAL
157
147.993
31.466
57.076
1.00
78.90
H


ATOM
2851
N
THR
158
148.395
29.520
58.122
1.00
68.08
H


ATOM
2852
CA
THR
158
148.282
30.116
59.442
1.00
63.24
H


ATOM
2853
CB
THR
158
147.209
29.396
60.279
1.00
61.67
H


ATOM
2854
OG1
THR
158
145.962
29.426
59.573
1.00
52.99
H


ATOM
2855
CG2
THR
158
147.034
30.079
61.632
1.00
56.86
H


ATOM
2856
C
THR
158
149.623
30.035
60.154
1.00
64.54
H


ATOM
2857
O
THR
158
150.187
28.954
60.312
1.00
65.02
H


ATOM
2858
N
VAL
159
150.131
31.190
60.571
1.00
48.72
H


ATOM
2859
CA
VAL
159
151.409
31.266
61.256
1.00
38.04
H


ATOM
2860
CB
VAL
159
152.384
32.209
60.517
1.00
35.68
H


ATOM
2861
CG1
VAL
159
153.745
32.207
61.195
1.00
13.69
H


ATOM
2862
CG2
VAL
159
152.520
31.765
59.072
1.00
35.33
H


ATOM
2863
C
VAL
159
151.239
31.746
62.690
1.00
32.37
H


ATOM
2864
O
VAL
159
150.940
32.917
62.951
1.00
46.22
H


ATOM
2865
N
THR
160
151.430
30.812
63.616
1.00
34.81
H


ATOM
2866
CA
THR
160
151.316
31.088
65.037
1.00
30.02
H


ATOM
2867
CB
THR
160
150.305
30.146
65.702
1.00
29.19
H


ATOM
2868
OG1
THR
160
149.788
29.230
64.729
1.00
41.86
H


ATOM
2869
CG2
THR
160
149.165
30.943
66.295
1.00
40.64
H


ATOM
2870
C
THR
160
152.676
30.872
65.681
1.00
28.21
H


ATOM
2871
O
THR
160
153.501
30.118
65.169
1.00
44.55
H


ATOM
2872
N
TRP
161
152.909
31.546
66.800
1.00
43.52
H


ATOM
2873
CA
TRP
161
154.171
31.404
67.506
1.00
54.28
H


ATOM
2874
CB
TRP
161
154.833
32.773
67.672
1.00
46.91
H


ATOM
2875
CG
TRP
161
155.369
33.327
66.379
1.00
31.10
H


ATOM
2876
CD2
TRP
161
156.648
33.059
65.790
1.00
39.01
H


ATOM
2877
CE2
TRP
161
156.710
33.782
64.579
1.00
43.01
H


ATOM
2878
CE3
TRP
161
157.748
32.277
66.169
1.00
31.39
H


ATOM
2879
CD1
TRP
161
154.727
34.179
65.525
1.00
30.94
H


ATOM
2880
NE1
TRP
161
155.525
34.458
64.443
1.00
31.32
H


ATOM
2881
CZ2
TRP
161
157.829
33.749
63.741
1.00
35.89
H


ATOM
2882
CZ3
TRP
161
158.861
32.242
65.335
1.00
23.31
H


ATOM
2883
CH2
TRP
161
158.893
32.975
64.134
1.00
38.71
H


ATOM
2884
C
TRP
161
153.920
30.746
68.862
1.00
57.21
H


ATOM
2885
O
TRP
161
153.157
31.255
69.691
1.00
61.27
H


ATOM
2886
N
ASN
162
154.569
29.602
69.071
1.00
56.37
H


ATOM
2887
CA
ASN
162
154.426
28.829
70.298
1.00
50.72
H


ATOM
2888
CB
ASN
162
154.818
29.676
71.515
1.00
39.98
H


ATOM
2889
CG
ASN
162
156.331
29.771
71.698
1.00
45.87
H


ATOM
2890
OD1
ASN
162
157.103
29.298
70.854
1.00
61.54
H


ATOM
2891
ND2
ASN
162
156.760
30.385
72.801
1.00
49.15
H


ATOM
2892
C
ASN
162
152.979
28.365
70.399
1.00
44.59
H


ATOM
2893
O
ASN
162
152.387
28.329
71.479
1.00
33.04
H


ATOM
2894
N
SER
163
152.419
28.021
69.242
1.00
55.71
H


ATOM
2895
CA
SER
163
151.048
27.541
69.133
1.00
65.28
H


ATOM
2896
CB
SER
163
150.858
26.313
70.021
1.00
69.26
H


ATOM
2897
OG
SER
163
151.917
25.391
69.823
1.00
87.88
H


ATOM
2898
C
SER
163
149.993
28.592
69.468
1.00
64.07
H


ATOM
2899
O
SER
163
148.799
28.363
69.267
1.00
71.86
H


ATOM
2900
N
GLY
164
150.430
29.744
69.970
1.00
56.72
H


ATOM
2901
CA
GLY
164
149.490
30.798
70.308
1.00
44.67
H


ATOM
2902
C
GLY
164
149.953
31.629
71.482
1.00
45.89
H


ATOM
2903
O
GLY
164
149.611
32.807
71.597
1.00
41.13
H


ATOM
2904
N
SER
165
150.739
31.011
72.355
1.00
56.45
H


ATOM
2905
CA
SER
165
151.259
31.688
73.533
1.00
57.02
H


ATOM
2906
CB
SER
165
152.169
30.745
74.316
1.00
62.37
H


ATOM
2907
OG
SER
165
151.418
29.663
74.836
1.00
50.35
H


ATOM
2908
C
SER
165
152.020
32.945
73.153
1.00
57.97
H


ATOM
2909
O
SER
165
152.276
33.809
73.989
1.00
41.07
H


ATOM
2910
N
LEU
166
152.385
33.049
71.883
1.00
64.03
H


ATOM
2911
CA
LEU
166
153.102
34.227
71.421
1.00
56.74
H


ATOM
2912
CB
LEU
166
154.493
33.841
70.923
1.00
37.44
H


ATOM
2913
CG
LEU
166
155.626
34.088
71.922
1.00
41.79
H


ATOM
2914
CD1
LEU
166
156.948
33.782
71.254
1.00
36.73
H


ATOM
2915
CD2
LEU
166
155.598
35.532
72.416
1.00
58.45
H


ATOM
2916
C
LEU
166
152.318
34.920
70.319
1.00
60.22
H


ATOM
2917
O
LEU
166
152.399
34.547
69.147
1.00
63.00
H


ATOM
2918
N
SER
167
151.547
35.928
70.708
1.00
65.96
H


ATOM
2919
CA
SER
167
150.740
36.684
69.762
1.00
64.77
H


ATOM
2920
CB
SER
167
149.255
36.347
69.946
1.00
66.65
H


ATOM
2921
OG
SER
167
148.697
37.030
71.057
1.00
54.23
H


ATOM
2922
C
SER
167
150.978
38.172
69.977
1.00
65.40
H


ATOM
2923
O
SER
167
150.141
39.009
69.637
1.00
63.94
H


ATOM
2924
N
SER
168
152.134
38.486
70.548
1.00
72.22
H


ATOM
2925
CA
SER
168
152.515
39.868
70.814
1.00
66.97
H


ATOM
2926
CB
SER
168
152.814
40.052
72.306
1.00
83.10
H


ATOM
2927
OG
SER
168
153.476
41.282
72.551
1.00
92.96
H


ATOM
2928
C
SER
168
153.754
40.212
69.997
1.00
58.05
H


ATOM
2929
O
SER
168
154.694
39.422
69.922
1.00
58.07
H


ATOM
2930
N
GLY
169
153.754
41.392
69.381
1.00
49.62
H


ATOM
2931
CA
GLY
169
154.897
41.799
68.582
1.00
43.99
H


ATOM
2932
C
GLY
169
155.088
40.916
67.366
1.00
44.05
H


ATOM
2933
O
GLY
169
156.205
40.731
66.887
1.00
49.35
H


ATOM
2934
N
VAL
170
153.991
40.366
66.862
1.00
45.75
H


ATOM
2935
CA
VAL
170
154.058
39.506
65.697
1.00
34.35
H


ATOM
2936
CB
VAL
170
153.135
38.291
65.854
1.00
29.09
H


ATOM
2937
CG1
VAL
170
153.213
37.418
64.614
1.00
23.76
H


ATOM
2938
CG2
VAL
170
153.539
37.492
67.079
1.00
30.40
H


ATOM
2939
C
VAL
170
153.660
40.269
64.445
1.00
37.22
H


ATOM
2940
O
VAL
170
152.732
41.074
64.460
1.00
35.69
H


ATOM
2941
N
HIS
171
154.383
40.017
63.364
1.00
38.69
H


ATOM
2942
CA
HIS
171
154.118
40.664
62.088
1.00
33.69
H


ATOM
2943
CB
HIS
171
155.167
41.743
61.802
1.00
38.36
H


ATOM
2944
CG
HIS
171
155.011
42.971
62.636
1.00
40.32
H


ATOM
2945
CD2
HIS
171
155.806
44.055
62.783
1.00
49.35
H


ATOM
2946
ND1
HIS
171
153.923
43.182
63.456
1.00
58.85
H


ATOM
2947
CE1
HIS
171
154.053
44.343
64.068
1.00
58.33
H


ATOM
2948
NE2
HIS
171
155.189
44.896
63.678
1.00
51.33
H


ATOM
2949
C
HIS
171
154.172
39.623
60.985
1.00
37.90
H


ATOM
2950
O
HIS
171
155.251
39.144
60.626
1.00
52.25
H


ATOM
2951
N
THR
172
153.012
39.250
60.465
1.00
36.40
H


ATOM
2952
CA
THR
172
152.974
38.289
59.379
1.00
37.99
H


ATOM
2953
CB
THR
172
151.865
37.251
59.576
1.00
30.37
H


ATOM
2954
OG1
THR
172
152.160
36.452
60.729
1.00
16.22
H


ATOM
2955
CG2
THR
172
151.760
36.351
58.356
1.00
18.72
H


ATOM
2956
C
THR
172
152.708
39.097
58.118
1.00
43.29
H


ATOM
2957
O
THR
172
151.690
39.782
58.011
1.00
42.87
H


ATOM
2958
N
PHE
173
153.635
39.031
57.172
1.00
42.03
H


ATOM
2959
CA
PHE
173
153.500
39.783
55.935
1.00
41.59
H


ATOM
2960
CB
PHE
173
154.889
40.102
55.372
1.00
69.43
H


ATOM
2961
CG
PHE
173
155.707
40.945
56.285
1.00
69.16
H


ATOM
2962
CD1
PHE
173
155.577
42.326
56.264
1.00
69.14
H


ATOM
2963
CD2
PHE
173
156.555
40.360
57.216
1.00
71.48
H


ATOM
2964
CE1
PHE
173
156.277
43.117
57.165
1.00
47.16
H


ATOM
2965
CE2
PHE
173
157.260
41.140
58.122
1.00
46.77
H


ATOM
2966
CZ
PHE
173
157.120
42.523
58.097
1.00
50.06
H


ATOM
2967
C
PHE
173
152.651
39.103
54.870
1.00
51.94
H


ATOM
2968
O
PHE
173
152.670
37.881
54.715
1.00
45.37
H


ATOM
2969
N
PRO
174
151.873
39.901
54.128
1.00
47.56
H


ATOM
2970
CD
PRO
174
151.760
41.360
54.268
1.00
40.08
H


ATOM
2971
CA
PRO
174
151.010
39.369
53.070
1.00
39.95
H


ATOM
2972
CB
PRO
174
150.417
40.618
52.419
1.00
38.02
H


ATOM
2973
CG
PRO
174
151.234
41.778
52.945
1.00
41.70
H


ATOM
2974
C
PRO
174
151.793
38.515
52.080
1.00
34.30
H


ATOM
2975
O
PRO
174
152.926
38.844
51.719
1.00
38.61
H


ATOM
2976
N
ALA
175
151.191
37.407
51.655
1.00
33.98
H


ATOM
2977
CA
ALA
175
151.834
36.505
50.711
1.00
28.24
H


ATOM
2978
CB
ALA
175
150.947
35.303
50.455
1.00
24.04
H


ATOM
2979
C
ALA
175
152.106
37.226
49.411
1.00
43.10
H


ATOM
2980
O
ALA
175
151.420
38.182
49.064
1.00
41.83
H


ATOM
2981
N
VAL
176
153.119
36.758
48.691
1.00
47.34
H


ATOM
2982
CA
VAL
176
153.479
37.340
47.409
1.00
46.04
H


ATOM
2983
CB
VAL
176
154.660
38.335
47.546
1.00
48.76
H


ATOM
2984
CG1
VAL
176
155.822
37.686
48.280
1.00
50.38
H


ATOM
2985
CG2
VAL
176
155.100
38.809
46.167
1.00
64.07
H


ATOM
2986
C
VAL
176
153.858
36.205
46.462
1.00
36.12
H


ATOM
2987
O
VAL
176
154.544
35.263
46.857
1.00
30.73
H


ATOM
2988
N
LEU
177
153.392
36.298
45.220
1.00
48.02
H


ATOM
2989
CA
LEU
177
153.647
35.282
44.205
1.00
60.32
H


ATOM
2990
CB
LEU
177
152.686
35.467
43.036
1.00
39.51
H


ATOM
2991
CG
LEU
177
152.048
34.165
42.557
1.00
27.99
H


ATOM
2992
CD1
LEU
177
151.014
33.698
43.572
1.00
30.57
H


ATOM
2993
CD2
LEU
177
151.410
34.378
41.197
1.00
38.71
H


ATOM
2994
C
LEU
177
155.074
35.257
43.679
1.00
73.61
H


ATOM
2995
O
LEU
177
155.621
36.275
43.254
1.00
78.42
H


ATOM
2996
N
GLN
178
155.669
34.074
43.694
1.00
79.08
H


ATOM
2997
CA
GLN
178
157.031
33.895
43.227
1.00
95.82
H


ATOM
2998
CB
GLN
178
157.981
33.932
44.432
1.00
99.99
H


ATOM
2999
CG
GLN
178
159.244
33.098
44.308
1.00
99.99
H


ATOM
3000
CD
GLN
178
160.357
33.583
45.224
1.00
99.99
H


ATOM
3001
OE1
GLN
178
160.327
33.367
46.439
1.00
98.52
H


ATOM
3002
NE2
GLN
178
161.350
34.244
44.637
1.00
99.99
H


ATOM
3003
C
GLN
178
157.123
32.564
42.483
1.00
99.99
H


ATOM
3004
O
GLN
178
157.289
31.504
43.092
1.00
99.97
H


ATOM
3005
N
SER
179
156.984
32.637
41.159
1.00
96.75
H


ATOM
3006
CA
SER
179
157.047
31.465
40.289
1.00
87.63
H


ATOM
3007
CB
SER
179
158.307
30.641
40.589
1.00
93.06
H


ATOM
3008
OG
SER
179
159.098
30.472
39.425
1.00
67.71
H


ATOM
3009
C
SER
179
155.815
30.580
40.430
1.00
81.94
H


ATOM
3010
O
SER
179
155.927
29.351
40.461
1.00
85.05
H


ATOM
3011
N
ASP
180
154.649
31.217
40.517
1.00
61.79
H


ATOM
3012
CA
ASP
180
153.367
30.518
40.643
1.00
53.42
H


ATOM
3013
CB
ASP
180
153.284
29.372
39.633
1.00
63.39
H


ATOM
3014
CG
ASP
180
152.902
29.846
38.251
1.00
90.41
H


ATOM
3015
OD1
ASP
180
151.736
30.262
38.066
1.00
95.28
H


ATOM
3016
OD2
ASP
180
153.771
29.804
37.350
1.00
91.35
H


ATOM
3017
C
ASP
180
153.036
29.975
42.030
1.00
48.83
H


ATOM
3018
O
ASP
180
151.965
29.414
42.234
1.00
32.55
H


ATOM
3019
N
LEU
181
153.955
30.120
42.976
1.00
49.73
H


ATOM
3020
CA
LEU
181
153.716
29.646
44.332
1.00
42.63
H


ATOM
3021
CB
LEU
181
154.751
28.594
44.730
1.00
51.61
H


ATOM
3022
CG
LEU
181
154.817
27.335
43.865
1.00
45.19
H


ATOM
3023
CD1
LEU
181
155.788
26.358
44.503
1.00
36.88
H


ATOM
3024
CD2
LEU
181
153.429
26.712
43.723
1.00
49.90
H


ATOM
3025
C
LEU
181
153.811
30.840
45.265
1.00
36.09
H


ATOM
3026
O
LEU
181
154.401
31.859
44.914
1.00
47.48
H


ATOM
3027
N
TYR
182
153.235
30.711
46.454
1.00
34.48
H


ATOM
3028
CA
TYR
182
153.254
31.803
47.410
1.00
34.99
H


ATOM
3029
CB
TYR
182
151.965
31.824
48.221
1.00
43.15
H


ATOM
3030
CG
TYR
182
150.768
32.266
47.431
1.00
26.23
H


ATOM
3031
CD1
TYR
182
150.514
33.621
47.219
1.00
24.46
H


ATOM
3032
CE1
TYR
182
149.437
34.034
46.455
1.00
26.76
H


ATOM
3033
CD2
TYR
182
149.908
31.332
46.864
1.00
28.50
H


ATOM
3034
CE2
TYR
182
148.827
31.733
46.097
1.00
40.52
H


ATOM
3035
CZ
TYR
182
148.596
33.085
45.893
1.00
31.63
H


ATOM
3036
OH
TYR
182
147.538
33.480
45.108
1.00
52.38
H


ATOM
3037
C
TYR
182
154.410
31.694
48.361
1.00
34.67
H


ATOM
3038
O
TYR
182
154.902
30.604
48.642
1.00
30.57
H


ATOM
3039
N
THR
183
154.842
32.844
48.860
1.00
46.64
H


ATOM
3040
CA
THR
183
155.915
32.914
49.838
1.00
32.69
H


ATOM
3041
CB
THR
183
157.250
33.348
49.232
1.00
32.26
H


ATOM
3042
OG1
THR
183
157.678
32.396
48.250
1.00
39.58
H


ATOM
3043
CG2
THR
183
158.288
33.442
50.318
1.00
33.99
H


ATOM
3044
C
THR
183
155.483
33.981
50.823
1.00
28.00
H


ATOM
3045
O
THR
183
154.927
35.009
50.440
1.00
38.20
H


ATOM
3046
N
LEU
184
155.737
33.743
52.097
1.00
27.54
H


ATOM
3047
CA
LEU
184
155.353
34.696
53.114
1.00
30.19
H


ATOM
3048
CB
LEU
184
153.926
34.380
53.559
1.00
30.95
H


ATOM
3049
CG
LEU
184
153.483
34.617
54.996
1.00
46.42
H


ATOM
3050
CD1
LEU
184
151.969
34.814
55.016
1.00
25.32
H


ATOM
3051
CD2
LEU
184
153.891
33.439
55.867
1.00
45.00
H


ATOM
3052
C
LEU
184
156.337
34.598
54.271
1.00
30.88
H


ATOM
3053
O
LEU
184
157.075
33.623
54.383
1.00
17.03
H


ATOM
3054
N
SER
185
156.370
35.613
55.122
1.00
29.51
H


ATOM
3055
CA
SER
185
157.278
35.599
56.252
1.00
24.01
H


ATOM
3056
CB
SER
185
158.573
36.350
55.914
1.00
21.45
H


ATOM
3057
OG
SER
185
158.354
37.749
55.844
1.00
46.01
H


ATOM
3058
C
SER
185
156.610
36.235
57.449
1.00
28.20
H


ATOM
3059
O
SER
185
155.828
37.173
57.312
1.00
26.88
H


ATOM
3060
N
SER
186
156.917
35.710
58.625
1.00
38.58
H


ATOM
3061
CA
SER
186
156.354
36.229
59.853
1.00
34.31
H


ATOM
3062
CB
SER
186
155.389
35.210
60.463
1.00
37.09
H


ATOM
3063
OG
SER
186
154.862
35.685
61.689
1.00
44.43
H


ATOM
3064
C
SER
186
157.487
36.510
60.822
1.00
25.01
H


ATOM
3065
O
SER
186
158.356
35.663
61.036
1.00
38.29
H


ATOM
3066
N
SER
187
157.491
37.703
61.395
1.00
15.70
H


ATOM
3067
CA
SER
187
158.524
38.066
62.348
1.00
25.23
H


ATOM
3068
CB
SER
187
159.100
39.440
62.008
1.00
18.98
H


ATOM
3069
OG
SER
187
158.187
40.466
62.346
1.00
36.58
H


ATOM
3070
C
SER
187
157.905
38.092
63.739
1.00
27.63
H


ATOM
3071
O
SER
187
156.692
38.240
63.882
1.00
26.02
H


ATOM
3072
N
VAL
188
158.739
37.926
64.762
1.00
31.12
H


ATOM
3073
CA
VAL
188
158.266
37.950
66.141
1.00
31.94
H


ATOM
3074
CB
VAL
188
157.996
36.524
66.681
1.00
32.97
H


ATOM
3075
CG1
VAL
188
159.298
35.776
66.878
1.00
21.32
H


ATOM
3076
CG2
VAL
188
157.221
36.606
67.987
1.00
48.09
H


ATOM
3077
C
VAL
188
159.284
38.646
67.030
1.00
29.00
H


ATOM
3078
O
VAL
188
160.469
38.322
67.016
1.00
18.81
H


ATOM
3079
N
THR
189
158.811
39.618
67.799
1.00
43.09
H


ATOM
3080
CA
THR
189
159.664
40.385
68.691
1.00
52.62
H


ATOM
3081
CB
THR
189
159.381
41.890
68.535
1.00
55.64
H


ATOM
3082
OG1
THR
189
159.915
42.346
67.288
1.00
72.06
H


ATOM
3083
CG2
THR
189
160.006
42.678
69.676
1.00
47.82
H


ATOM
3084
C
THR
189
159.450
39.981
70.147
1.00
47.05
H


ATOM
3085
O
THR
189
158.313
39.914
70.622
1.00
51.99
H


ATOM
3086
N
VAL
190
160.547
39.715
70.846
1.00
33.42
H


ATOM
3087
CA
VAL
190
160.489
39.327
72.251
1.00
49.20
H


ATOM
3088
CB
VAL
190
160.501
37.795
72.408
1.00
46.79
H


ATOM
3089
CG1
VAL
190
159.222
37.205
71.839
1.00
24.58
H


ATOM
3090
CG2
VAL
190
161.717
37.217
71.715
1.00
27.47
H


ATOM
3091
C
VAL
190
161.670
39.893
73.037
1.00
57.65
H


ATOM
3092
O
VAL
190
162.797
39.935
72.538
1.00
35.32
H


ATOM
3093
N
PRO
191
161.419
40.347
74.277
1.00
74.09
H


ATOM
3094
CD
PRO
191
160.096
40.363
74.931
1.00
85.05
H


ATOM
3095
CA
PRO
191
162.461
40.912
75.142
1.00
73.28
H


ATOM
3096
CB
PRO
191
161.769
41.033
76.496
1.00
85.43
H


ATOM
3097
CG
PRO
191
160.328
41.206
76.155
1.00
90.65
H


ATOM
3098
C
PRO
191
163.708
40.031
75.207
1.00
69.24
H


ATOM
3099
O
PRO
191
163.618
38.814
75.392
1.00
45.94
H


ATOM
3100
N
SER
192
164.871
40.663
75.063
1.00
75.06
H


ATOM
3101
CA
SER
192
166.146
39.952
75.082
1.00
71.45
H


ATOM
3102
CB
SER
192
167.311
40.955
75.103
1.00
59.57
H


ATOM
3103
OG
SER
192
167.154
41.923
76.129
1.00
83.36
H


ATOM
3104
C
SER
192
166.291
38.977
76.248
1.00
74.48
H


ATOM
3105
O
SER
192
167.095
38.051
76.184
1.00
78.55
H


ATOM
3106
N
SER
193
165.498
39.169
77.297
1.00
78.60
H


ATOM
3107
CA
SER
193
165.574
38.315
78.476
1.00
75.62
H


ATOM
3108
CB
SER
193
164.901
39.019
79.661
1.00
88.02
H


ATOM
3109
OG
SER
193
163.490
39.027
79.525
1.00
99.66
H


ATOM
3110
C
SER
193
165.013
36.898
78.345
1.00
67.52
H


ATOM
3111
O
SER
193
165.430
35.998
79.073
1.00
55.75
H


ATOM
3112
N
THR
194
164.083
36.684
77.418
1.00
72.49
H


ATOM
3113
CA
THR
194
163.464
35.365
77.259
1.00
71.00
H


ATOM
3114
CB
THR
194
161.967
35.507
76.936
1.00
85.22
H


ATOM
3115
OG1
THR
194
161.795
36.425
75.846
1.00
87.88
H


ATOM
3116
CG2
THR
194
161.213
36.023
78.154
1.00
86.98
H


ATOM
3117
C
THR
194
164.082
34.432
76.219
1.00
51.89
H


ATOM
3118
O
THR
194
163.726
33.253
76.150
1.00
40.90
H


ATOM
3119
N
TRP
195
164.996
34.957
75.408
1.00
50.33
H


ATOM
3120
CA
TRP
195
165.654
34.154
74.381
1.00
48.60
H


ATOM
3121
CB
TRP
195
165.109
34.515
72.990
1.00
43.12
H


ATOM
3122
CG
TRP
195
165.338
33.444
71.945
1.00
50.92
H


ATOM
3123
CD2
TRP
195
166.332
33.451
70.907
1.00
56.48
H


ATOM
3124
CE2
TRP
195
166.199
32.235
70.192
1.00
47.66
H


ATOM
3125
CE3
TRP
195
167.320
34.365
70.513
1.00
53.35
H


ATOM
3126
CD1
TRP
195
164.664
32.259
71.817
1.00
57.45
H


ATOM
3127
NE1
TRP
195
165.175
31.528
70.766
1.00
58.35
H


ATOM
3128
CZ2
TRP
195
167.019
31.911
69.104
1.00
45.39
H


ATOM
3129
CZ3
TRP
195
168.136
34.040
69.430
1.00
48.84
H


ATOM
3130
CH2
TRP
195
167.979
32.821
68.741
1.00
37.86
H


ATOM
3131
C
TRP
195
167.164
34.383
74.420
1.00
55.91
H


ATOM
3132
O
TRP
195
167.620
35.492
74.702
1.00
67.87
H


ATOM
3133
N
PRO
196
167.960
33.334
74.143
1.00
55.52
H


ATOM
3134
CD
PRO
196
169.426
33.449
74.122
1.00
48.86
H


ATOM
3135
CA
PRO
196
167.526
31.972
73.804
1.00
46.54
H


ATOM
3136
CB
PRO
196
168.764
31.339
73.154
1.00
39.17
H


ATOM
3137
CG
PRO
196
169.812
32.444
73.087
1.00
48.88
H


ATOM
3138
C
PRO
196
167.047
31.179
75.016
1.00
48.13
H


ATOM
3139
O
PRO
196
166.920
29.951
74.958
1.00
51.32
H


ATOM
3140
N
SER
197
166.789
31.891
76.109
1.00
63.32
H


ATOM
3141
CA
SER
197
166.315
31.282
77.343
1.00
62.46
H


ATOM
3142
CB
SER
197
165.718
32.353
78.255
1.00
69.33
H


ATOM
3143
OG
SER
197
166.324
32.329
79.533
1.00
75.74
H


ATOM
3144
C
SER
197
165.261
30.225
77.029
1.00
55.85
H


ATOM
3145
O
SER
197
165.557
29.029
76.979
1.00
63.63
H


ATOM
3146
N
GLU
198
164.032
30.680
76.809
1.00
50.23
H


ATOM
3147
CA
GLU
198
162.925
29.787
76.494
1.00
57.31
H


ATOM
3148
CB
GLU
198
161.605
30.416
76.945
1.00
72.95
H


ATOM
3149
CG
GLU
198
161.645
30.972
78.360
1.00
75.15
H


ATOM
3150
CD
GLU
198
160.646
32.093
78.584
1.00
85.11
H


ATOM
3151
OE1
GLU
198
159.817
32.341
77.680
1.00
99.04
H


ATOM
3152
OE2
GLU
198
160.689
32.725
79.664
1.00
77.93
H


ATOM
3153
C
GLU
198
162.889
29.516
74.995
1.00
56.54
H


ATOM
3154
O
GLU
198
163.247
30.384
74.201
1.00
63.21
H


ATOM
3155
N
THR
199
162.462
28.314
74.614
1.00
54.93
H


ATOM
3156
CA
THR
199
162.392
27.937
73.203
1.00
53.51
H


ATOM
3157
CB
THR
199
162.304
26.400
73.031
1.00
61.18
H


ATOM
3158
OG1
THR
199
161.128
25.903
73.681
1.00
51.25
H


ATOM
3159
CG2
THR
199
163.536
25.727
73.623
1.00
78.07
H


ATOM
3160
C
THR
199
161.205
28.583
72.485
1.00
59.63
H


ATOM
3161
O
THR
199
160.120
28.730
73.054
1.00
59.98
H


ATOM
3162
N
VAL
200
161.429
28.966
71.228
1.00
57.25
H


ATOM
3163
CA
VAL
200
160.407
29.603
70.405
1.00
51.96
H


ATOM
3164
CB
VAL
200
160.815
31.039
70.035
1.00
33.42
H


ATOM
3165
CG1
VAL
200
159.951
31.540
68.899
1.00
17.75
H


ATOM
3166
CG2
VAL
200
160.680
31.944
71.239
1.00
31.97
H


ATOM
3167
C
VAL
200
160.195
28.832
69.112
1.00
51.65
H


ATOM
3168
O
VAL
200
161.136
28.613
68.353
1.00
62.16
H


ATOM
3169
N
THR
201
158.955
28.427
68.862
1.00
39.44
H


ATOM
3170
CA
THR
201
158.631
27.684
67.648
1.00
32.31
H


ATOM
3171
CB
THR
201
158.116
26.267
67.969
1.00
41.49
H


ATOM
3172
OG1
THR
201
159.042
25.602
68.831
1.00
51.63
H


ATOM
3173
CG2
THR
201
157.949
25.458
66.688
1.00
39.87
H


ATOM
3174
C
THR
201
157.545
28.399
66.860
1.00
26.58
H


ATOM
3175
O
THR
201
156.682
29.055
67.438
1.00
25.18
H


ATOM
3176
N
CYS
202
157.593
28.279
65.538
1.00
22.39
H


ATOM
3177
CA
CYS
202
156.582
28.902
64.699
1.00
25.43
H


ATOM
3178
C
CYS
202
155.765
27.777
64.064
1.00
25.96
H


ATOM
3179
O
CYS
202
156.324
26.826
63.522
1.00
34.22
H


ATOM
3180
CB
CYS
202
157.243
29.820
63.649
1.00
49.34
H


ATOM
3181
SG
CYS
202
157.564
29.150
61.983
1.00
74.97
H


ATOM
3182
N
ASN
203
154.441
27.871
64.172
1.00
24.52
H


ATOM
3183
CA
ASN
203
153.554
26.842
63.628
1.00
29.46
H


ATOM
3184
CB
ASN
203
152.539
26.387
64.686
1.00
36.69
H


ATOM
3185
CG
ASN
203
152.926
26.807
66.099
1.00
44.66
H


ATOM
3186
OD1
ASN
203
152.586
27.904
66.552
1.00
28.02
H


ATOM
3187
ND2
ASN
203
153.629
25.926
66.805
1.00
33.32
H


ATOM
3188
C
ASN
203
152.800
27.293
62.389
1.00
18.74
H


ATOM
3189
O
ASN
203
151.964
28.196
62.447
1.00
38.03
H


ATOM
3190
N
VAL
204
153.099
26.646
61.270
1.00
23.45
H


ATOM
3191
CA
VAL
204
152.451
26.957
60.006
1.00
38.87
H


ATOM
3192
CB
VAL
204
153.473
27.049
58.853
1.00
39.50
H


ATOM
3193
CG1
VAL
204
152.795
27.575
57.599
1.00
52.52
H


ATOM
3194
CG2
VAL
204
154.624
27.944
59.254
1.00
29.99
H


ATOM
3195
C
VAL
204
151.461
25.854
59.676
1.00
41.89
H


ATOM
3196
O
VAL
204
151.733
24.682
59.919
1.00
46.77
H


ATOM
3197
N
ALA
205
150.312
26.236
59.126
1.00
41.52
H


ATOM
3198
CA
ALA
205
149.285
25.273
58.751
1.00
32.14
H


ATOM
3199
CB
ALA
205
148.156
25.281
59.777
1.00
44.74
H


ATOM
3200
C
ALA
205
148.749
25.614
57.365
1.00
28.16
H


ATOM
3201
O
ALA
205
148.349
26.750
57.104
1.00
23.06
H


ATOM
3202
N
HIS
206
148.756
24.629
56.474
1.00
38.89
H


ATOM
3203
CA
HIS
206
148.267
24.825
55.121
1.00
31.01
H


ATOM
3204
CB
HIS
206
149.357
24.497
54.112
1.00
34.08
H


ATOM
3205
CG
HIS
206
149.025
24.909
52.715
1.00
25.51
H


ATOM
3206
CD2
HIS
206
148.521
26.065
52.227
1.00
33.37
H


ATOM
3207
ND1
HIS
206
149.193
24.073
51.635
1.00
28.36
H


ATOM
3208
CE1
HIS
206
148.807
24.698
50.535
1.00
30.36
H


ATOM
3209
NE2
HIS
206
148.393
25.907
50.868
1.00
29.53
H


ATOM
3210
C
HIS
206
147.061
23.930
54.876
1.00
38.48
H


ATOM
3211
O
HIS
206
147.198
22.819
54.371
1.00
42.18
H


ATOM
3212
N
PRO
207
145.856
24.417
55.228
1.00
46.11
H


ATOM
3213
CD
PRO
207
145.636
25.758
55.802
1.00
59.24
H


ATOM
3214
CA
PRO
207
144.597
23.684
55.065
1.00
49.09
H


ATOM
3215
CB
PRO
207
143.538
24.780
55.138
1.00
55.68
H


ATOM
3216
CG
PRO
207
144.146
25.814
56.018
1.00
65.42
H


ATOM
3217
C
PRO
207
144.504
22.890
53.769
1.00
60.16
H


ATOM
3218
O
PRO
207
144.293
21.680
53.787
1.00
51.48
H


ATOM
3219
N
ALA
208
144.664
23.577
52.646
1.00
56.11
H


ATOM
3220
CA
ALA
208
144.584
22.933
51.342
1.00
41.51
H


ATOM
3221
CB
ALA
208
145.043
23.899
50.261
1.00
34.52
H


ATOM
3222
C
ALA
208
145.397
21.638
51.269
1.00
43.41
H


ATOM
3223
O
ALA
208
144.983
20.676
50.615
1.00
51.37
H


ATOM
3224
N
SER
209
146.550
21.615
51.933
1.00
47.62
H


ATOM
3225
CA
SER
209
147.396
20.424
51.923
1.00
48.72
H


ATOM
3226
CB
SER
209
148.852
20.811
51.634
1.00
44.67
H


ATOM
3227
OG
SER
209
149.387
21.642
52.650
1.00
55.80
H


ATOM
3228
C
SER
209
147.304
19.643
53.236
1.00
53.29
H


ATOM
3229
O
SER
209
147.973
18.624
53.411
1.00
52.35
H


ATOM
3230
N
SER
210
146.462
20.119
54.148
1.00
71.17
H


ATOM
3231
CA
SER
210
146.269
19.475
55.444
1.00
82.90
H


ATOM
3232
CB
SER
210
145.509
18.158
55.277
1.00
90.95
H


ATOM
3233
OG
SER
210
144.137
18.333
55.578
1.00
100.00
H


ATOM
3234
C
SER
210
147.579
19.213
56.169
1.00
79.59
H


ATOM
3235
O
SER
210
147.702
18.234
56.908
1.00
80.94
H


ATOM
3236
N
THR
211
148.555
20.088
55.950
1.00
73.59
H


ATOM
3237
CA
THR
211
149.854
19.964
56.594
1.00
75.14
H


ATOM
3238
CB
THR
211
151.027
20.111
55.599
1.00
76.28
H


ATOM
3239
OG1
THR
211
150.990
21.414
55.008
1.00
73.09
H


ATOM
3240
CG2
THR
211
150.950
19.062
54.510
1.00
89.67
H


ATOM
3241
C
THR
211
150.024
21.051
57.643
1.00
74.45
H


ATOM
3242
O
THR
211
149.536
22.172
57.491
1.00
76.65
H


ATOM
3243
N
LYS
212
150.711
20.699
58.722
1.00
69.16
H


ATOM
3244
CA
LYS
212
150.988
21.645
59.783
1.00
61.67
H


ATOM
3245
CB
LYS
212
150.041
21.450
60.967
1.00
73.38
H


ATOM
3246
CG
LYS
212
150.718
21.612
62.317
1.00
89.85
H


ATOM
3247
CD
LYS
212
150.006
22.614
63.192
1.00
99.99
H


ATOM
3248
CE
LYS
212
150.452
22.449
64.637
1.00
97.52
H


ATOM
3249
NZ
LYS
212
150.308
23.707
65.419
1.00
95.76
H


ATOM
3250
C
LYS
212
152.413
21.344
60.198
1.00
51.18
H


ATOM
3251
O
LYS
212
152.730
20.222
60.594
1.00
46.57
H


ATOM
3252
N
VAL
213
153.275
22.349
60.081
1.00
47.52
H


ATOM
3253
CA
VAL
213
154.672
22.201
60.434
1.00
33.06
H


ATOM
3254
CB
VAL
213
155.593
22.651
59.282
1.00
35.68
H


ATOM
3255
CG1
VAL
213
157.002
22.136
59.511
1.00
51.97
H


ATOM
3256
CG2
VAL
213
155.047
22.154
57.948
1.00
53.21
H


ATOM
3257
C
VAL
213
155.011
23.028
61.661
1.00
30.06
H


ATOM
3258
O
VAL
213
154.324
23.996
61.991
1.00
34.34
H


ATOM
3259
N
ASP
214
156.071
22.625
62.343
1.00
35.26
H


ATOM
3260
CA
ASP
214
156.541
23.325
63.523
1.00
39.19
H


ATOM
3261
CB
ASP
214
156.228
22.526
64.791
1.00
58.41
H


ATOM
3262
CG
ASP
214
154.791
22.709
65.259
1.00
63.54
H


ATOM
3263
OD1
ASP
214
154.397
23.857
65.553
1.00
65.10
H


ATOM
3264
OD2
ASP
214
154.059
21.699
65.336
1.00
80.45
H


ATOM
3265
C
ASP
214
158.046
23.454
63.342
1.00
37.64
H


ATOM
3266
O
ASP
214
158.725
22.489
62.983
1.00
41.00
H


ATOM
3267
N
LYS
215
158.564
24.652
63.564
1.00
34.85
H


ATOM
3268
CA
LYS
215
159.985
24.877
63.410
1.00
35.83
H


ATOM
3269
CB
LYS
215
160.261
25.637
62.112
1.00
37.75
H


ATOM
3270
CG
LYS
215
161.248
24.948
61.191
1.00
59.89
H


ATOM
3271
CD
LYS
215
160.536
23.992
60.251
1.00
59.31
H


ATOM
3272
CE
LYS
215
161.386
22.766
59.964
1.00
53.98
H


ATOM
3273
NZ
LYS
215
160.573
21.613
59.475
1.00
65.45
H


ATOM
3274
C
LYS
215
160.515
25.659
64.588
1.00
38.73
H


ATOM
3275
O
LYS
215
160.030
26.745
64.904
1.00
53.68
H


ATOM
3276
N
LYS
216
161.511
25.084
65.244
1.00
48.31
H


ATOM
3277
CA
LYS
216
162.141
25.714
66.389
1.00
47.35
H


ATOM
3278
CB
LYS
216
162.649
24.641
67.357
1.00
48.55
H


ATOM
3279
CG
LYS
216
162.043
23.256
67.119
1.00
74.84
H


ATOM
3280
CD
LYS
216
162.861
22.156
67.793
1.00
85.40
H


ATOM
3281
CE
LYS
216
162.592
22.085
69.296
1.00
94.97
H


ATOM
3282
NZ
LYS
216
162.133
20.733
69.738
1.00
92.19
H


ATOM
3283
C
LYS
216
163.305
26.543
65.860
1.00
46.24
H


ATOM
3284
O
LYS
216
163.898
26.203
64.838
1.00
34.41
H


ATOM
3285
N
ILE
217
163.622
27.637
66.541
1.00
41.02
H


ATOM
3286
CA
ILE
217
164.719
28.497
66.116
1.00
44.97
H


ATOM
3287
CB
ILE
217
164.273
29.978
66.046
1.00
41.86
H


ATOM
3288
CG2
ILE
217
165.094
30.716
64.991
1.00
44.48
H


ATOM
3289
CG1
ILE
217
162.768
30.063
65.749
1.00
33.66
H


ATOM
3290
CD1
ILE
217
162.407
30.038
64.270
1.00
17.95
H


ATOM
3291
C
ILE
217
165.890
28.363
67.094
1.00
47.47
H


ATOM
3292
O
ILE
217
165.690
28.400
68.310
1.00
43.27
H


ATOM
3293
N
VAL
218
167.103
28.219
66.559
1.00
50.40
H


ATOM
3294
CA
VAL
218
168.301
28.054
67.394
1.00
50.95
H


ATOM
3295
CB
VAL
218
169.003
26.705
67.084
1.00
57.08
H


ATOM
3296
CG1
VAL
218
167.974
25.686
66.599
1.00
55.44
H


ATOM
3297
CG2
VAL
218
170.105
26.903
66.037
1.00
73.39
H


ATOM
3298
C
VAL
218
169.344
29.175
67.289
1.00
41.93
H


ATOM
3299
O
VAL
218
170.001
29.458
68.317
1.00
46.19
H


ATOM
3300
OT
VAL
218
170.011
29.390
69.119
1.00
52.14
H


ATOM
3301
CB
LYS
2
95.854
32.588
47.685
1.00
42.79
C


ATOM
3302
CG
LYS
2
95.825
31.435
46.699
1.00
53.80
C


ATOM
3303
CD
LYS
2
94.407
30.925
46.486
1.00
62.43
C


ATOM
3304
CE
LYS
2
94.373
29.740
45.531
1.00
62.95
C


ATOM
3305
NZ
LYS
2
94.065
28.460
46.232
1.00
60.28
C


ATOM
3306
C
LYS
2
94.693
33.099
49.840
1.00
46.91
C


ATOM
3307
O
LYS
2
93.606
32.673
50.239
1.00
58.66
C


ATOM
3308
N
LYS
2
95.259
30.740
49.234
1.00
34.71
C


ATOM
3309
CA
LYS
2
95.690
32.169
49.150
1.00
47.34
C


ATOM
3310
N
ILE
3
95.072
34.368
49.975
1.00
39.77
C


ATOM
3311
CA
ILE
3
94.226
35.372
50.621
1.00
41.61
C


ATOM
3312
CB
ILE
3
95.062
36.456
51.331
1.00
43.12
C


ATOM
3313
CG2
ILE
3
94.142
37.421
52.074
1.00
41.52
C


ATOM
3314
CG1
ILE
3
96.056
35.818
52.301
1.00
48.11
C


ATOM
3315
CD1
ILE
3
97.282
36.672
52.556
1.00
29.73
C


ATOM
3316
C
ILE
3
93.346
36.101
49.623
1.00
38.09
C


ATOM
3317
O
ILE
3
93.645
36.147
48.437
1.00
41.06
C


ATOM
3318
N
LEU
4
92.266
36.686
50.124
1.00
41.63
C


ATOM
3319
CA
LEU
4
91.349
37.439
49.287
1.00
47.21
C


ATOM
3320
CB
LEU
4
89.906
37.026
49.581
1.00
47.90
C


ATOM
3321
CG
LEU
4
89.389
35.782
48.852
1.00
49.25
C


ATOM
3322
CD1
LEU
4
89.552
35.970
47.351
1.00
37.24
C


ATOM
3323
CD2
LEU
4
90.138
34.543
49.324
1.00
54.80
C


ATOM
3324
C
LEU
4
91.536
38.927
49.569
1.00
48.08
C


ATOM
3325
O
LEU
4
91.868
39.322
50.695
1.00
31.46
C


ATOM
3326
N
VAL
5
91.326
39.743
48.538
1.00
43.17
C


ATOM
3327
CA
VAL
5
91.469
41.190
48.660
1.00
33.62
C


ATOM
3328
CB
VAL
5
92.806
41.673
48.047
1.00
32.71
C


ATOM
3329
CG1
VAL
5
93.315
42.880
48.803
1.00
22.52
C


ATOM
3330
CG2
VAL
5
93.836
40.559
48.081
1.00
29.21
C


ATOM
3331
C
VAL
5
90.325
41.911
47.945
1.00
36.98
C


ATOM
3332
O
VAL
5
90.028
41.628
46.788
1.00
50.54
C


ATOM
3333
N
LYS
6
89.689
42.844
48.640
1.00
39.70
C


ATOM
3334
CA
LYS
6
88.593
43.622
48.068
1.00
41.54
C


ATOM
3335
CB
LYS
6
87.299
43.378
48.857
1.00
38.28
C


ATOM
3336
CG
LYS
6
86.443
42.233
48.341
1.00
48.43
C


ATOM
3337
CD
LYS
6
85.343
41.841
49.339
1.00
58.62
C


ATOM
3338
CE
LYS
6
85.905
41.158
50.593
1.00
47.34
C


ATOM
3339
NZ
LYS
6
86.645
39.893
50.281
1.00
51.47
C


ATOM
3340
C
LYS
6
88.957
45.108
48.147
1.00
32.95
C


ATOM
3341
O
LYS
6
89.265
45.614
49.222
1.00
43.88
C


ATOM
3342
N
GLN
7
88.926
45.805
47.021
1.00
30.15
C


ATOM
3343
CA
GLN
7
89.258
47.224
47.022
1.00
39.07
C


ATOM
3344
CB
GLN
7
90.631
47.449
46.395
1.00
44.76
C


ATOM
3345
CG
GLN
7
91.355
46.178
46.002
1.00
48.85
C


ATOM
3346
CD
GLN
7
92.745
46.462
45.467
1.00
53.95
C


ATOM
3347
OE1
GLN
7
93.575
45.560
45.334
1.00
50.12
C


ATOM
3348
NE2
GLN
7
93.007
47.730
45.155
1.00
35.66
C


ATOM
3349
C
GLN
7
88.230
48.035
46.262
1.00
36.89
C


ATOM
3350
O
GLN
7
87.567
47.514
45.370
1.00
42.41
C


ATOM
3351
N
SER
8
88.095
49.307
46.623
1.00
34.89
C


ATOM
3352
CA
SER
8
87.148
50.178
45.940
1.00
33.64
C


ATOM
3353
CB
SER
8
87.247
51.613
46.475
1.00
39.10
C


ATOM
3354
OG
SER
8
86.259
51.882
47.465
1.00
33.65
C


ATOM
3355
C
SER
8
87.564
50.141
44.481
1.00
36.57
C


ATOM
3356
O
SER
8
88.751
50.012
44.180
1.00
34.54
C


ATOM
3357
N
PRO
9
86.596
50.239
43.553
1.00
45.61
C


ATOM
3358
CD
PRO
9
85.146
50.390
43.755
1.00
42.54
C


ATOM
3359
CA
PRO
9
86.946
50.207
42.128
1.00
42.76
C


ATOM
3360
CB
PRO
9
85.594
50.325
41.411
1.00
35.11
C


ATOM
3361
CG
PRO
9
84.577
49.955
42.428
1.00
29.77
C


ATOM
3362
C
PRO
9
87.879
51.370
41.813
1.00
45.27
C


ATOM
3363
O
PRO
9
88.929
51.194
41.190
1.00
49.59
C


ATOM
3364
N
MET
10
87.486
52.561
42.254
1.00
34.14
C


ATOM
3365
CA
MET
10
88.292
53.749
42.041
1.00
36.35
C


ATOM
3366
CB
MET
10
87.975
54.374
40.674
1.00
51.67
C


ATOM
3367
CG
MET
10
87.958
55.908
40.628
1.00
45.29
C


ATOM
3368
SD
MET
10
86.852
56.596
39.357
1.00
41.23
C


ATOM
3369
CE
MET
10
88.067
57.323
38.239
1.00
36.20
C


ATOM
3370
C
MET
10
88.031
54.730
43.172
1.00
31.46
C


ATOM
3371
O
MET
10
87.032
54.629
43.882
1.00
22.69
C


ATOM
3372
N
LEU
11
88.962
55.661
43.344
1.00
34.82
C


ATOM
3373
CA
LEU
11
88.890
56.669
44.385
1.00
23.35
C


ATOM
3374
CB
LEU
11
89.826
56.264
45.530
1.00
20.95
C


ATOM
3375
CG
LEU
11
89.592
54.804
45.946
1.00
18.02
C


ATOM
3376
CD1
LEU
11
90.765
54.235
46.696
1.00
5.04
C


ATOM
3377
CD2
LEU
11
88.339
54.745
46.785
1.00
31.87
C


ATOM
3378
C
LEU
11
89.306
58.008
43.789
1.00
22.25
C


ATOM
3379
O
LEU
11
90.294
58.093
43.061
1.00
23.22
C


ATOM
3380
N
VAL
12
88.540
59.051
44.075
1.00
23.39
C


ATOM
3381
CA
VAL
12
88.871
60.365
43.551
1.00
25.58
C


ATOM
3382
CB
VAL
12
87.610
61.166
43.174
1.00
37.83
C


ATOM
3383
CG1
VAL
12
88.008
62.509
42.585
1.00
20.17
C


ATOM
3384
CG2
VAL
12
86.771
60.387
42.173
1.00
25.83
C


ATOM
3385
C
VAL
12
89.655
61.133
44.603
1.00
30.45
C


ATOM
3386
O
VAL
12
89.106
61.605
45.597
1.00
47.50
C


ATOM
3387
N
ALA
13
90.955
61.246
44.379
1.00
37.89
C


ATOM
3388
CA
ALA
13
91.820
61.943
45.307
1.00
26.16
C


ATOM
3389
CB
ALA
13
93.203
62.091
44.706
1.00
30.65
C


ATOM
3390
C
ALA
13
91.241
63.309
45.615
1.00
33.79
C


ATOM
3391
O
ALA
13
90.816
64.028
44.711
1.00
39.57
C


ATOM
3392
N
TYR
14
91.206
63.657
46.895
1.00
39.35
C


ATOM
3393
CA
TYR
14
90.711
64.959
47.306
1.00
37.04
C


ATOM
3394
CB
TYR
14
89.660
64.835
48.410
1.00
33.79
C


ATOM
3395
CG
TYR
14
89.555
66.110
49.213
1.00
48.57
C


ATOM
3396
CD1
TYR
14
90.182
66.233
50.458
1.00
50.52
C


ATOM
3397
CE1
TYR
14
90.178
67.444
51.148
1.00
54.90
C


ATOM
3398
CD2
TYR
14
88.913
67.231
48.685
1.00
44.13
C


ATOM
3399
CE2
TYR
14
88.904
68.441
49.364
1.00
49.92
C


ATOM
3400
CZ
TYR
14
89.538
68.545
50.590
1.00
58.83
C


ATOM
3401
OH
TYR
14
89.536
69.761
51.240
1.00
69.05
C


ATOM
3402
C
TYR
14
91.884
65.765
47.846
1.00
39.91
C


ATOM
3403
O
TYR
14
92.626
65.283
48.703
1.00
36.85
C


ATOM
3404
N
ASP
15
92.041
66.992
47.359
1.00
34.60
C


ATOM
3405
CA
ASP
15
93.131
67.838
47.820
1.00
35.97
C


ATOM
3406
CB
ASP
15
92.958
68.141
49.307
1.00
54.21
C


ATOM
3407
CG
ASP
15
92.605
69.588
49.573
1.00
74.11
C


ATOM
3408
OD1
ASP
15
92.300
70.322
48.604
1.00
77.84
C


ATOM
3409
OD2
ASP
15
92.634
69.985
50.758
1.00
75.11
C


ATOM
3410
C
ASP
15
94.448
67.112
47.603
1.00
33.47
C


ATOM
3411
O
ASP
15
95.389
67.265
48.380
1.00
34.49
C


ATOM
3412
N
ASN
16
94.499
66.309
46.543
1.00
44.74
C


ATOM
3413
CA
ASN
16
95.683
65.534
46.196
1.00
48.87
C


ATOM
3414
CB
ASN
16
96.898
66.457
46.053
1.00
62.07
C


ATOM
3415
CG
ASN
16
96.840
67.292
44.793
1.00
64.94
C


ATOM
3416
OD1
ASN
16
96.213
68.355
44.760
1.00
66.06
C


ATOM
3417
ND2
ASN
16
97.497
66.816
43.742
1.00
73.29
C


ATOM
3418
C
ASN
16
95.983
64.449
47.225
1.00
48.29
C


ATOM
3419
O
ASN
16
97.148
64.096
47.451
1.00
38.81
C


ATOM
3420
N
ALA
17
94.923
63.923
47.835
1.00
34.08
C


ATOM
3421
CA
ALA
17
95.055
62.867
48.840
1.00
29.57
C


ATOM
3422
CB
ALA
17
94.996
63.459
50.237
1.00
33.14
C


ATOM
3423
C
ALA
17
93.955
61.832
48.667
1.00
29.96
C


ATOM
3424
O
ALA
17
92.931
62.107
48.049
1.00
32.16
C


ATOM
3425
N
VAL
18
94.169
60.642
49.213
1.00
28.09
C


ATOM
3426
CA
VAL
18
93.188
59.568
49.111
1.00
30.54
C


ATOM
3427
CB
VAL
18
93.339
58.824
47.767
1.00
15.07
C


ATOM
3428
CG1
VAL
18
94.517
57.889
47.829
1.00
23.83
C


ATOM
3429
CG2
VAL
18
92.086
58.054
47.448
1.00
16.29
C


ATOM
3430
C
VAL
18
93.356
58.575
50.266
1.00
33.43
C


ATOM
3431
O
VAL
18
94.314
58.660
51.029
1.00
28.92
C


ATOM
3432
N
ASN
19
92.419
57.643
50.398
1.00
30.17
C


ATOM
3433
CA
ASN
19
92.496
56.642
51.456
1.00
28.35
C


ATOM
3434
CB
ASN
19
91.468
56.959
52.545
1.00
37.98
C


ATOM
3435
CG
ASN
19
92.105
57.540
53.794
1.00
36.06
C


ATOM
3436
OD1
ASN
19
92.991
56.923
54.385
1.00
36.77
C


ATOM
3437
ND2
ASN
19
91.659
58.732
54.205
1.00
27.02
C


ATOM
3438
C
ASN
19
92.241
55.260
50.867
1.00
28.23
C


ATOM
3439
O
ASN
19
91.113
54.941
50.502
1.00
25.92
C


ATOM
3440
N
LEU
20
93.291
54.446
50.781
1.00
37.11
C


ATOM
3441
CA
LEU
20
93.192
53.097
50.204
1.00
39.33
C


ATOM
3442
CB
LEU
20
94.382
52.830
49.284
1.00
52.73
C


ATOM
3443
CG
LEU
20
94.409
53.492
47.913
1.00
59.34
C


ATOM
3444
CD1
LEU
20
95.375
54.657
47.953
1.00
54.35
C


ATOM
3445
CD2
LEU
20
94.826
52.478
46.855
1.00
58.17
C


ATOM
3446
C
LEU
20
93.122
51.946
51.202
1.00
24.88
C


ATOM
3447
O
LEU
20
94.141
51.349
51.532
1.00
27.29
C


ATOM
3448
N
SER
21
91.926
51.619
51.670
1.00
24.25
C


ATOM
3449
CA
SER
21
91.795
50.515
52.601
1.00
32.93
C


ATOM
3450
CB
SER
21
90.474
50.603
53.357
1.00
22.84
C


ATOM
3451
OG
SER
21
90.626
50.123
54.682
1.00
42.82
C


ATOM
3452
C
SER
21
91.834
49.250
51.760
1.00
36.87
C


ATOM
3453
O
SER
21
91.633
49.308
50.551
1.00
50.83
C


ATOM
3454
N
CYS
22
92.098
48.109
52.384
1.00
41.84
C


ATOM
3455
CA
CYS
22
92.142
46.858
51.636
1.00
34.97
C


ATOM
3456
C
CYS
22
91.519
45.728
52.420
1.00
29.54
C


ATOM
3457
O
CYS
22
92.086
45.250
53.400
1.00
31.51
C


ATOM
3458
CB
CYS
22
93.578
46.504
51.294
1.00
44.42
C


ATOM
3459
SG
CYS
22
94.382
47.674
50.162
1.00
54.15
C


ATOM
3460
N
LYS
23
90.339
45.312
51.983
1.00
9.94
C


ATOM
3461
CA
LYS
23
89.630
44.234
52.639
1.00
27.48
C


ATOM
3462
CB
LYS
23
88.196
44.149
52.099
1.00
45.60
C


ATOM
3463
CG
LYS
23
87.083
44.123
53.160
1.00
46.91
C


ATOM
3464
CD
LYS
23
85.742
44.572
52.558
1.00
49.03
C


ATOM
3465
CE
LYS
23
84.537
43.911
53.219
1.00
44.60
C


ATOM
3466
NZ
LYS
23
83.321
43.966
52.347
1.00
31.56
C


ATOM
3467
C
LYS
23
90.373
42.933
52.354
1.00
28.04
C


ATOM
3468
O
LYS
23
90.727
42.651
51.209
1.00
27.74
C


ATOM
3469
N
TYR
24
90.616
42.154
53.404
1.00
48.19
C


ATOM
3470
CA
TYR
24
91.305
40.869
53.279
1.00
51.31
C


ATOM
3471
CB
TYR
24
92.585
40.864
54.121
1.00
33.41
C


ATOM
3472
CG
TYR
24
93.582
41.914
53.708
1.00
28.69
C


ATOM
3473
CD1
TYR
24
94.190
41.867
52.454
1.00
41.70
C


ATOM
3474
CE1
TYR
24
95.089
42.846
52.049
1.00
20.51
C


ATOM
3475
CD2
TYR
24
93.901
42.971
54.554
1.00
29.67
C


ATOM
3476
CE2
TYR
24
94.803
43.960
54.159
1.00
21.03
C


ATOM
3477
CZ
TYR
24
95.389
43.889
52.905
1.00
18.10
C


ATOM
3478
OH
TYR
24
96.263
44.862
52.495
1.00
37.76
C


ATOM
3479
C
TYR
24
90.403
39.736
53.749
1.00
47.73
C


ATOM
3480
O
TYR
24
89.264
39.957
54.151
1.00
49.24
C


ATOM
3481
N
SER
25
90.920
38.518
53.690
1.00
57.38
C


ATOM
3482
CA
SER
25
90.175
37.353
54.146
1.00
60.28
C


ATOM
3483
CB
SER
25
89.888
36.407
52.975
1.00
59.25
C


ATOM
3484
OG
SER
25
91.068
36.142
52.238
1.00
69.79
C


ATOM
3485
C
SER
25
91.076
36.668
55.164
1.00
54.52
C


ATOM
3486
O
SER
25
91.290
35.455
55.108
1.00
60.55
C


ATOM
3487
N
TYR
26
91.606
37.458
56.096
1.00
43.18
C


ATOM
3488
CA
TYR
26
92.516
36.933
57.101
1.00
31.88
C


ATOM
3489
CB
TYR
26
93.949
36.995
56.566
1.00
26.62
C


ATOM
3490
CG
TYR
26
94.845
35.878
57.060
1.00
37.63
C


ATOM
3491
CD1
TYR
26
95.606
36.026
58.220
1.00
43.69
C


ATOM
3492
CE1
TYR
26
96.443
35.003
58.673
1.00
50.14
C


ATOM
3493
CD2
TYR
26
94.941
34.677
56.363
1.00
43.23
C


ATOM
3494
CE2
TYR
26
95.776
33.646
56.809
1.00
62.28
C


ATOM
3495
CZ
TYR
26
96.524
33.816
57.964
1.00
56.86
C


ATOM
3496
OH
TYR
26
97.352
32.801
58.404
1.00
54.48
C


ATOM
3497
C
TYR
26
92.455
37.637
58.451
1.00
30.34
C


ATOM
3498
O
TYR
26
92.275
38.851
58.518
1.00
12.16
C


ATOM
3499
N
ASN
27
92.626
36.832
59.504
1.00
35.16
C


ATOM
3500
CA
ASN
27
92.639
37.230
60.920
1.00
43.53
C


ATOM
3501
CB
ASN
27
94.092
37.461
61.380
1.00
44.74
C


ATOM
3502
CG
ASN
27
94.595
38.869
61.095
1.00
54.71
C


ATOM
3503
OD1
ASN
27
93.890
39.691
60.503
1.00
49.86
C


ATOM
3504
ND2
ASN
27
95.833
39.153
61.521
1.00
38.35
C


ATOM
3505
C
ASN
27
91.760
38.379
61.413
1.00
52.07
C


ATOM
3506
O
ASN
27
91.255
39.159
60.582
1.00
64.58
C


ATOM
3507
OT
ASN
27
91.583
38.478
62.651
1.00
38.64
C


ATOM
3508
CB
SER
30
98.029
39.101
66.273
1.00
38.67
C


ATOM
3509
OG
SER
30
99.164
39.940
66.371
1.00
28.74
C


ATOM
3510
C
SER
30
99.198
37.741
64.527
1.00
48.62
C


ATOM
3511
O
SER
30
99.534
36.748
65.163
1.00
42.19
C


ATOM
3512
N
SER
30
96.758
37.537
64.847
1.00
40.61
C


ATOM
3513
CA
SER
30
97.910
38.480
64.881
1.00
46.89
C


ATOM
3514
N
ARG
31
99.906
38.226
63.505
1.00
52.76
C


ATOM
3515
CA
ARG
31
101.156
37.606
63.056
1.00
48.62
C


ATOM
3516
CB
ARG
31
100.864
36.509
62.014
1.00
48.21
C


ATOM
3517
CG
ARG
31
99.390
36.316
61.654
1.00
45.83
C


ATOM
3518
CD
ARG
31
99.123
34.913
61.098
1.00
45.48
C


ATOM
3519
NE
ARG
31
98.202
34.159
61.946
1.00
56.80
C


ATOM
3520
CZ
ARG
31
97.819
32.904
61.723
1.00
60.88
C


ATOM
3521
NH1
ARG
31
98.273
32.238
60.666
1.00
53.06
C


ATOM
3522
NH2
ARG
31
96.980
32.312
62.565
1.00
65.41
C


ATOM
3523
C
ARG
31
102.140
38.620
62.457
1.00
42.34
C


ATOM
3524
O
ARG
31
102.024
39.821
62.696
1.00
37.05
C


ATOM
3525
N
GLU
32
103.109
38.119
61.688
1.00
33.63
C


ATOM
3526
CA
GLU
32
104.114
38.959
61.035
1.00
28.55
C


ATOM
3527
CB
GLU
32
105.510
38.366
61.211
1.00
35.76
C


ATOM
3528
CG
GLU
32
106.639
39.289
60.762
1.00
44.46
C


ATOM
3529
CD
GLU
32
107.993
38.584
60.704
1.00
47.94
C


ATOM
3530
OE1
GLU
32
108.183
37.588
61.436
1.00
50.54
C


ATOM
3531
OE2
GLU
32
108.870
39.024
59.931
1.00
43.16
C


ATOM
3532
C
GLU
32
103.777
39.047
59.550
1.00
32.96
C


ATOM
3533
O
GLU
32
103.876
38.061
58.815
1.00
19.87
C


ATOM
3534
N
PHE
33
103.394
40.244
59.122
1.00
41.41
C


ATOM
3535
CA
PHE
33
102.989
40.488
57.747
1.00
35.05
C


ATOM
3536
CB
PHE
33
101.494
40.691
57.715
1.00
26.59
C


ATOM
3537
CG
PHE
33
101.065
41.944
58.408
1.00
24.17
C


ATOM
3538
CD1
PHE
33
101.369
43.192
57.861
1.00
23.40
C


ATOM
3539
CD2
PHE
33
100.374
41.889
59.611
1.00
15.64
C


ATOM
3540
CE1
PHE
33
100.989
44.367
58.496
1.00
28.02
C


ATOM
3541
CE2
PHE
33
99.984
43.063
60.260
1.00
24.23
C


ATOM
3542
CZ
PHE
33
100.292
44.304
59.703
1.00
31.35
C


ATOM
3543
C
PHE
33
103.640
41.716
57.121
1.00
34.54
C


ATOM
3544
O
PHE
33
104.134
42.613
57.813
1.00
21.74
C


ATOM
3545
N
ARG
34
103.564
41.771
55.797
1.00
28.28
C


ATOM
3546
CA
ARG
34
104.145
42.864
55.045
1.00
21.13
C


ATOM
3547
CB
ARG
34
105.469
42.413
54.437
1.00
12.61
C


ATOM
3548
CG
ARG
34
106.189
43.483
53.643
1.00
19.39
C


ATOM
3549
CD
ARG
34
106.709
42.916
52.339
1.00
18.93
C


ATOM
3550
NE
ARG
34
108.026
43.440
51.995
1.00
36.83
C


ATOM
3551
CZ
ARG
34
108.251
44.344
51.045
1.00
40.83
C


ATOM
3552
NH1
ARG
34
107.239
44.825
50.340
1.00
45.97
C


ATOM
3553
NH2
ARG
34
109.490
44.752
50.785
1.00
38.52
C


ATOM
3554
C
ARG
34
103.202
43.309
53.940
1.00
32.95
C


ATOM
3555
O
ARG
34
103.239
42.779
52.831
1.00
41.93
C


ATOM
3556
N
ALA
35
102.362
44.294
54.239
1.00
34.63
C


ATOM
3557
CA
ALA
35
101.416
44.798
53.251
1.00
26.09
C


ATOM
3558
CB
ALA
35
100.381
45.657
53.921
1.00
26.37
C


ATOM
3559
C
ALA
35
102.134
45.599
52.173
1.00
28.19
C


ATOM
3560
O
ALA
35
103.201
46.163
52.414
1.00
19.20
C


ATOM
3561
N
SER
36
101.545
45.641
50.980
1.00
36.87
C


ATOM
3562
CA
SER
36
102.136
46.374
49.863
1.00
39.36
C


ATOM
3563
CB
SER
36
103.172
45.488
49.160
1.00
51.82
C


ATOM
3564
OG
SER
36
103.705
44.516
50.050
1.00
62.97
C


ATOM
3565
C
SER
36
101.099
46.886
48.847
1.00
32.13
C


ATOM
3566
O
SER
36
100.092
46.238
48.575
1.00
28.44
C


ATOM
3567
N
LEU
37
101.364
48.069
48.304
1.00
34.51
C


ATOM
3568
CA
LEU
37
100.496
48.705
47.320
1.00
31.61
C


ATOM
3569
CB
LEU
37
99.897
49.987
47.895
1.00
36.64
C


ATOM
3570
CG
LEU
37
98.943
50.801
47.023
1.00
21.97
C


ATOM
3571
CD1
LEU
37
97.533
50.720
47.578
1.00
21.64
C


ATOM
3572
CD2
LEU
37
99.412
52.231
46.991
1.00
26.43
C


ATOM
3573
C
LEU
37
101.393
49.042
46.148
1.00
28.60
C


ATOM
3574
O
LEU
37
102.341
49.811
46.295
1.00
35.19
C


ATOM
3575
N
HIS
38
101.112
48.454
44.993
1.00
22.34
C


ATOM
3576
CA
HIS
38
101.935
48.694
43.816
1.00
21.00
C


ATOM
3577
CB
HIS
38
102.418
47.370
43.232
1.00
29.80
C


ATOM
3578
CG
HIS
38
103.038
46.464
44.248
1.00
41.65
C


ATOM
3579
CD2
HIS
38
104.324
46.102
44.458
1.00
36.66
C


ATOM
3580
ND1
HIS
38
102.296
45.819
45.218
1.00
51.35
C


ATOM
3581
CE1
HIS
38
103.102
45.102
45.978
1.00
45.79
C


ATOM
3582
NE2
HIS
38
104.337
45.256
45.539
1.00
50.11
C


ATOM
3583
C
HIS
38
101.138
49.458
42.794
1.00
20.21
C


ATOM
3584
O
HIS
38
99.935
49.263
42.679
1.00
24.62
C


ATOM
3585
N
LYS
39
101.807
50.334
42.055
1.00
17.62
C


ATOM
3586
CA
LYS
39
101.135
51.154
41.065
1.00
28.42
C


ATOM
3587
CB
LYS
39
101.458
52.627
41.334
1.00
33.83
C


ATOM
3588
CG
LYS
39
100.938
53.579
40.281
1.00
47.76
C


ATOM
3589
CD
LYS
39
102.046
54.496
39.767
1.00
56.33
C


ATOM
3590
CE
LYS
39
101.922
54.727
38.264
1.00
52.05
C


ATOM
3591
NZ
LYS
39
101.573
56.133
37.920
1.00
39.26
C


ATOM
3592
C
LYS
39
101.527
50.784
39.644
1.00
37.38
C


ATOM
3593
O
LYS
39
102.715
50.697
39.323
1.00
38.26
C


ATOM
3594
N
GLY
40
100.528
50.559
38.793
1.00
33.72
C


ATOM
3595
CA
GLY
40
100.819
50.230
37.412
1.00
30.90
C


ATOM
3596
C
GLY
40
100.183
48.957
36.913
1.00
33.65
C


ATOM
3597
O
GLY
40
100.025
47.997
37.665
1.00
26.93
C


ATOM
3598
N
LEU
41
99.819
48.962
35.634
1.00
48.13
C


ATOM
3599
CA
LEU
41
99.196
47.809
34.988
1.00
43.35
C


ATOM
3600
CB
LEU
41
98.598
48.211
33.633
1.00
41.37
C


ATOM
3601
CG
LEU
41
97.256
47.613
33.178
1.00
50.66
C


ATOM
3602
CD1
LEU
41
96.622
46.783
34.283
1.00
41.32
C


ATOM
3603
CD2
LEU
41
96.324
48.742
32.756
1.00
36.16
C


ATOM
3604
C
LEU
41
100.270
46.758
34.791
1.00
44.95
C


ATOM
3605
O
LEU
41
99.976
45.585
34.577
1.00
48.29
C


ATOM
3606
N
ASP
42
101.522
47.197
34.841
1.00
51.52
C


ATOM
3607
CA
ASP
42
102.648
46.285
34.713
1.00
53.82
C


ATOM
3608
CB
ASP
42
103.666
46.804
33.690
1.00
44.60
C


ATOM
3609
CG
ASP
42
104.352
48.090
34.125
1.00
49.55
C


ATOM
3610
OD1
ASP
42
103.874
48.765
35.063
1.00
40.26
C


ATOM
3611
OD2
ASP
42
105.385
48.427
33.510
1.00
48.33
C


ATOM
3612
C
ASP
42
103.249
46.241
36.106
1.00
57.65
C


ATOM
3613
O
ASP
42
104.394
45.836
36.298
1.00
57.61
C


ATOM
3614
N
SER
43
102.433
46.679
37.065
1.00
57.54
C


ATOM
3615
CA
SER
43
102.767
46.741
38.486
1.00
56.75
C


ATOM
3616
CB
SER
43
102.299
45.467
39.177
1.00
40.78
C


ATOM
3617
OG
SER
43
100.896
45.502
39.359
1.00
32.86
C


ATOM
3618
C
SER
43
104.234
46.995
38.803
1.00
59.27
C


ATOM
3619
O
SER
43
104.795
46.413
39.732
1.00
57.66
C


ATOM
3620
N
ALA
44
104.847
47.884
38.033
1.00
54.64
C


ATOM
3621
CA
ALA
44
106.244
48.215
38.224
1.00
41.67
C


ATOM
3622
CB
ALA
44
106.654
49.295
37.239
1.00
41.38
C


ATOM
3623
C
ALA
44
106.514
48.679
39.646
1.00
42.01
C


ATOM
3624
O
ALA
44
107.073
47.935
40.449
1.00
48.39
C


ATOM
3625
N
VAL
45
106.083
49.904
39.943
1.00
40.52
C


ATOM
3626
CA
VAL
45
106.290
50.563
41.239
1.00
34.97
C


ATOM
3627
CB
VAL
45
106.036
52.073
41.094
1.00
27.80
C


ATOM
3628
CG1
VAL
45
106.126
52.753
42.441
1.00
32.20
C


ATOM
3629
CG2
VAL
45
107.036
52.674
40.126
1.00
35.71
C


ATOM
3630
C
VAL
45
105.526
50.100
42.489
1.00
35.47
C


ATOM
3631
O
VAL
45
104.307
49.956
42.462
1.00
45.80
C


ATOM
3632
N
GLU
46
106.259
49.893
43.585
1.00
39.87
C


ATOM
3633
CA
GLU
46
105.666
49.503
44.868
1.00
41.71
C


ATOM
3634
CB
GLU
46
106.546
48.488
45.615
1.00
50.85
C


ATOM
3635
CG
GLU
46
106.163
48.251
47.094
1.00
34.64
C


ATOM
3636
CD
GLU
46
106.638
46.895
47.637
1.00
57.55
C


ATOM
3637
OE1
GLU
46
107.869
46.712
47.809
1.00
47.66
C


ATOM
3638
OE2
GLU
46
105.783
46.014
47.893
1.00
29.27
C


ATOM
3639
C
GLU
46
105.575
50.791
45.680
1.00
37.48
C


ATOM
3640
O
GLU
46
106.530
51.189
46.338
1.00
43.67
C


ATOM
3641
N
VAL
47
104.418
51.433
45.614
1.00
25.13
C


ATOM
3642
CA
VAL
47
104.173
52.692
46.297
1.00
15.83
C


ATOM
3643
CB
VAL
47
102.736
53.146
46.037
1.00
19.37
C


ATOM
3644
CG1
VAL
47
102.428
54.405
46.826
1.00
28.90
C


ATOM
3645
CG2
VAL
47
102.543
53.374
44.547
1.00
26.86
C


ATOM
3646
C
VAL
47
104.437
52.717
47.800
1.00
29.17
C


ATOM
3647
O
VAL
47
105.070
53.641
48.310
1.00
31.30
C


ATOM
3648
N
CYS
48
103.952
51.709
48.512
1.00
40.43
C


ATOM
3649
CA
CYS
48
104.142
51.665
49.955
1.00
37.33
C


ATOM
3650
C
CYS
48
104.145
50.246
50.495
1.00
27.79
C


ATOM
3651
O
CYS
48
103.618
49.331
49.864
1.00
20.24
C


ATOM
3652
CB
CYS
48
103.035
52.459
50.644
1.00
57.93
C


ATOM
3653
SG
CYS
48
103.314
52.822
52.407
1.00
69.94
C


ATOM
3654
N
VAL
49
104.747
50.070
51.664
1.00
18.58
C


ATOM
3655
CA
VAL
49
104.802
48.764
52.290
1.00
20.44
C


ATOM
3656
CB
VAL
49
106.139
48.030
51.966
1.00
27.56
C


ATOM
3657
CG1
VAL
49
107.042
48.931
51.131
1.00
18.34
C


ATOM
3658
CG2
VAL
49
106.851
47.592
53.249
1.00
17.63
C


ATOM
3659
C
VAL
49
104.654
48.926
53.794
1.00
33.14
C


ATOM
3660
O
VAL
49
105.537
49.471
54.455
1.00
29.92
C


ATOM
3661
N
VAL
50
103.523
48.467
54.324
1.00
41.12
C


ATOM
3662
CA
VAL
50
103.255
48.539
55.759
1.00
44.37
C


ATOM
3663
CB
VAL
50
101.796
48.932
56.031
1.00
48.04
C


ATOM
3664
CG1
VAL
50
101.486
48.786
57.511
1.00
45.70
C


ATOM
3665
CG2
VAL
50
101.562
50.367
55.576
1.00
35.76
C


ATOM
3666
C
VAL
50
103.553
47.176
56.385
1.00
38.20
C


ATOM
3667
O
VAL
50
102.899
46.176
56.085
1.00
36.51
C


ATOM
3668
N
TYR
51
104.543
47.146
57.264
1.00
25.60
C


ATOM
3669
CA
TYR
51
104.957
45.898
57.879
1.00
34.11
C


ATOM
3670
CB
TYR
51
106.479
45.779
57.799
1.00
34.41
C


ATOM
3671
CG
TYR
51
107.060
44.744
58.724
1.00
31.19
C


ATOM
3672
CD1
TYR
51
107.617
45.109
59.944
1.00
38.77
C


ATOM
3673
CE1
TYR
51
108.122
44.153
60.822
1.00
44.07
C


ATOM
3674
CD2
TYR
51
107.025
43.396
58.395
1.00
33.29
C


ATOM
3675
CE2
TYR
51
107.525
42.432
59.265
1.00
41.62
C


ATOM
3676
CZ
TYR
51
108.068
42.818
60.477
1.00
39.08
C


ATOM
3677
OH
TYR
51
108.511
41.867
61.364
1.00
47.15
C


ATOM
3678
C
TYR
51
104.507
45.729
59.315
1.00
33.68
C


ATOM
3679
O
TYR
51
104.696
46.613
60.145
1.00
23.59
C


ATOM
3680
N
GLY
52
103.927
44.571
59.603
1.00
29.01
C


ATOM
3681
CA
GLY
52
103.459
44.300
60.947
1.00
30.05
C


ATOM
3682
C
GLY
52
104.067
43.041
61.527
1.00
39.73
C


ATOM
3683
O
GLY
52
104.393
42.096
60.806
1.00
40.35
C


ATOM
3684
N
ASN
53
104.224
43.032
62.843
1.00
46.66
C


ATOM
3685
CA
ASN
53
104.798
41.888
63.537
1.00
57.56
C


ATOM
3686
CB
ASN
53
106.308
42.073
63.721
1.00
32.73
C


ATOM
3687
CG
ASN
53
106.971
40.851
64.322
1.00
30.74
C


ATOM
3688
OD1
ASN
53
106.474
39.728
64.185
1.00
25.99
C


ATOM
3689
ND2
ASN
53
108.099
41.059
64.995
1.00
35.70
C


ATOM
3690
C
ASN
53
104.141
41.729
64.897
1.00
63.15
C


ATOM
3691
O
ASN
53
104.709
42.114
65.918
1.00
67.81
C


ATOM
3692
N
TYR
54
102.941
41.159
64.906
1.00
66.70
C


ATOM
3693
CA
TYR
54
102.213
40.953
66.149
1.00
70.87
C


ATOM
3694
CB
TYR
54
103.004
40.008
67.072
1.00
60.20
C


ATOM
3695
CG
TYR
54
103.185
38.604
66.521
1.00
54.18
C


ATOM
3696
CD1
TYR
54
104.380
38.215
65.923
1.00
49.72
C


ATOM
3697
CE1
TYR
54
104.538
36.931
65.393
1.00
51.50
C


ATOM
3698
CD2
TYR
54
102.149
37.672
66.579
1.00
63.25
C


ATOM
3699
CE2
TYR
54
102.297
36.386
66.052
1.00
53.22
C


ATOM
3700
CZ
TYR
54
103.489
36.025
65.461
1.00
51.26
C


ATOM
3701
OH
TYR
54
103.625
34.761
64.931
1.00
41.36
C


ATOM
3702
C
TYR
54
102.018
42.313
66.811
1.00
75.80
C


ATOM
3703
O
TYR
54
101.118
43.072
66.443
1.00
70.77
C


ATOM
3704
N
SER
55
102.870
42.617
67.784
1.00
83.17
C


ATOM
3705
CA
SER
55
102.805
43.892
68.483
1.00
89.21
C


ATOM
3706
CB
SER
55
103.373
43.750
69.896
1.00
89.77
C


ATOM
3707
OG
SER
55
102.332
43.662
70.853
1.00
90.69
C


ATOM
3708
C
SER
55
103.617
44.916
67.695
1.00
90.09
C


ATOM
3709
O
SER
55
103.181
45.378
66.639
1.00
92.24
C


ATOM
3710
N
GLN
56
104.796
45.251
68.218
1.00
90.35
C


ATOM
3711
CA
GLN
56
105.715
46.207
67.600
1.00
93.42
C


ATOM
3712
CB
GLN
56
106.882
45.448
66.957
1.00
91.24
C


ATOM
3713
CG
GLN
56
107.492
44.372
67.861
1.00
94.20
C


ATOM
3714
CD
GLN
56
108.069
43.192
67.088
1.00
99.99
C


ATOM
3715
OE1
GLN
56
108.128
42.066
67.595
1.00
87.06
C


ATOM
3716
NE2
GLN
56
108.500
43.446
65.854
1.00
99.99
C


ATOM
3717
C
GLN
56
105.040
47.121
66.572
1.00
96.11
C


ATOM
3718
O
GLN
56
104.866
46.744
65.413
1.00
99.98
C


ATOM
3719
N
GLN
57
104.670
48.323
67.013
1.00
90.45
C


ATOM
3720
CA
GLN
57
103.995
49.318
66.177
1.00
83.45
C


ATOM
3721
CB
GLN
57
104.417
50.732
66.589
1.00
85.60
C


ATOM
3722
CG
GLN
57
104.367
50.991
68.084
1.00
89.34
C


ATOM
3723
CD
GLN
57
105.744
50.966
68.718
1.00
87.16
C


ATOM
3724
OE1
GLN
57
106.497
51.937
68.635
1.00
76.99
C


ATOM
3725
NE2
GLN
57
106.080
49.849
69.355
1.00
90.12
C


ATOM
3726
C
GLN
57
104.185
49.175
64.669
1.00
80.35
C


ATOM
3727
O
GLN
57
105.278
48.877
64.182
1.00
77.96
C


ATOM
3728
N
LEU
58
103.095
49.401
63.943
1.00
76.47
C


ATOM
3729
CA
LEU
58
103.077
49.331
62.487
1.00
61.35
C


ATOM
3730
CB
LEU
58
101.723
49.838
61.972
1.00
52.59
C


ATOM
3731
CG
LEU
58
100.956
49.130
60.845
1.00
50.02
C


ATOM
3732
CD1
LEU
58
100.916
47.634
61.070
1.00
55.58
C


ATOM
3733
CD2
LEU
58
99.535
49.671
60.794
1.00
39.64
C


ATOM
3734
C
LEU
58
104.209
50.181
61.906
1.00
54.39
C


ATOM
3735
O
LEU
58
104.387
51.345
62.284
1.00
51.09
C


ATOM
3736
N
GLN
59
104.976
49.589
60.997
1.00
51.81
C


ATOM
3737
CA
GLN
59
106.080
50.290
60.346
1.00
46.97
C


ATOM
3738
CB
GLN
59
107.394
49.516
60.524
1.00
35.66
C


ATOM
3739
CG
GLN
59
108.469
50.260
61.308
1.00
32.42
C


ATOM
3740
CD
GLN
59
109.327
49.340
62.171
1.00
53.43
C


ATOM
3741
OE1
GLN
59
110.198
49.799
62.912
1.00
50.95
C


ATOM
3742
NE2
GLN
59
109.082
48.037
62.079
1.00
46.72
C


ATOM
3743
C
GLN
59
105.748
50.417
58.859
1.00
43.43
C


ATOM
3744
O
GLN
59
105.697
49.422
58.138
1.00
42.60
C


ATOM
3745
N
VAL
60
105.505
51.645
58.412
1.00
40.75
C


ATOM
3746
CA
VAL
60
105.172
51.907
57.018
1.00
39.80
C


ATOM
3747
CB
VAL
60
103.828
52.659
56.896
1.00
26.19
C


ATOM
3748
CG1
VAL
60
103.878
53.955
57.678
1.00
21.41
C


ATOM
3749
CG2
VAL
60
103.528
52.946
55.448
1.00
27.26
C


ATOM
3750
C
VAL
60
106.267
52.745
56.371
1.00
41.05
C


ATOM
3751
O
VAL
60
106.733
53.727
56.950
1.00
51.96
C


ATOM
3752
N
TYR
61
106.692
52.349
55.177
1.00
37.63
C


ATOM
3753
CA
TYR
61
107.731
53.086
54.477
1.00
25.83
C


ATOM
3754
CB
TYR
61
109.118
52.575
54.861
1.00
12.00
C


ATOM
3755
CG
TYR
61
109.496
51.237
54.274
1.00
18.91
C


ATOM
3756
CD1
TYR
61
109.216
50.053
54.953
1.00
42.23
C


ATOM
3757
CE1
TYR
61
109.599
48.819
54.439
1.00
33.05
C


ATOM
3758
CD2
TYR
61
110.173
51.150
53.057
1.00
38.49
C


ATOM
3759
CE2
TYR
61
110.562
49.918
52.533
1.00
23.48
C


ATOM
3760
CZ
TYR
61
110.269
48.758
53.231
1.00
29.34
C


ATOM
3761
OH
TYR
61
110.627
47.535
52.714
1.00
30.14
C


ATOM
3762
C
TYR
61
107.540
52.981
52.986
1.00
35.71
C


ATOM
3763
O
TYR
61
106.694
52.214
52.513
1.00
35.76
C


ATOM
3764
N
SER
62
108.322
53.775
52.258
1.00
39.08
C


ATOM
3765
CA
SER
62
108.268
53.813
50.805
1.00
39.42
C


ATOM
3766
CB
SER
62
107.247
54.857
50.345
1.00
32.31
C


ATOM
3767
OG
SER
62
107.120
54.853
48.932
1.00
23.48
C


ATOM
3768
C
SER
62
109.643
54.137
50.220
1.00
45.43
C


ATOM
3769
O
SER
62
110.287
55.116
50.601
1.00
42.20
C


ATOM
3770
N
LYS
63
110.094
53.302
49.294
1.00
50.45
C


ATOM
3771
CA
LYS
63
111.388
53.499
48.655
1.00
44.30
C


ATOM
3772
CB
LYS
63
112.040
52.136
48.373
1.00
41.16
C


ATOM
3773
CG
LYS
63
113.045
51.676
49.431
1.00
41.13
C


ATOM
3774
CD
LYS
63
112.720
50.288
49.969
1.00
36.52
C


ATOM
3775
CE
LYS
63
113.769
49.809
50.972
1.00
27.51
C


ATOM
3776
NZ
LYS
63
114.917
49.099
50.322
1.00
14.01
C


ATOM
3777
C
LYS
63
111.173
54.243
47.343
1.00
40.39
C


ATOM
3778
O
LYS
63
112.079
54.322
46.515
1.00
43.49
C


ATOM
3779
N
THR
64
109.977
54.802
47.166
1.00
38.77
C


ATOM
3780
CA
THR
64
109.635
55.496
45.926
1.00
33.97
C


ATOM
3781
CB
THR
64
108.407
54.837
45.264
1.00
45.65
C


ATOM
3782
OG1
THR
64
107.445
54.502
46.272
1.00
61.07
C


ATOM
3783
CG2
THR
64
108.811
53.576
44.520
1.00
45.91
C


ATOM
3784
C
THR
64
109.360
56.988
46.033
1.00
33.47
C


ATOM
3785
O
THR
64
109.122
57.651
45.023
1.00
25.44
C


ATOM
3786
N
GLY
65
109.375
57.525
47.244
1.00
35.21
C


ATOM
3787
CA
GLY
65
109.119
58.946
47.383
1.00
35.72
C


ATOM
3788
C
GLY
65
107.644
59.286
47.468
1.00
35.69
C


ATOM
3789
O
GLY
65
107.198
60.317
46.970
1.00
41.32
C


ATOM
3790
N
PHE
66
106.881
58.405
48.100
1.00
31.14
C


ATOM
3791
CA
PHE
66
105.454
58.616
48.280
1.00
22.44
C


ATOM
3792
CB
PHE
66
104.680
57.350
47.914
1.00
15.68
C


ATOM
3793
CG
PHE
66
104.307
57.256
46.463
1.00
27.36
C


ATOM
3794
CD1
PHE
66
105.243
56.859
45.514
1.00
38.42
C


ATOM
3795
CD2
PHE
66
103.009
57.536
46.042
1.00
42.24
C


ATOM
3796
CE1
PHE
66
104.889
56.738
44.159
1.00
42.03
C


ATOM
3797
CE2
PHE
66
102.648
57.419
44.694
1.00
38.62
C


ATOM
3798
CZ
PHE
66
103.590
57.018
43.750
1.00
28.00
C


ATOM
3799
C
PHE
66
105.238
58.921
49.758
1.00
29.60
C


ATOM
3800
O
PHE
66
105.701
58.176
50.622
1.00
28.41
C


ATOM
3801
N
ASN
67
104.569
60.028
50.053
1.00
29.26
C


ATOM
3802
CA
ASN
67
104.289
60.375
51.436
1.00
28.71
C


ATOM
3803
CB
ASN
67
103.776
61.814
51.516
1.00
44.50
C


ATOM
3804
CG
ASN
67
103.832
62.385
52.923
1.00
56.37
C


ATOM
3805
OD1
ASN
67
103.890
63.603
53.110
1.00
55.38
C


ATOM
3806
ND2
ASN
67
103.816
61.507
53.922
1.00
49.90
C


ATOM
3807
C
ASN
67
103.208
59.390
51.891
1.00
30.16
C


ATOM
3808
O
ASN
67
102.018
59.698
51.893
1.00
22.60
C


ATOM
3809
N
CYS
68
103.627
58.190
52.276
1.00
45.93
C


ATOM
3810
CA
CYS
68
102.678
57.165
52.691
1.00
37.84
C


ATOM
3811
C
CYS
68
102.480
56.998
54.198
1.00
40.89
C


ATOM
3812
O
CYS
68
103.414
57.122
54.986
1.00
26.35
C


ATOM
3813
CB
CYS
68
103.087
55.824
52.089
1.00
23.40
C


ATOM
3814
SG
CYS
68
102.108
54.426
52.713
1.00
53.33
C


ATOM
3815
N
ASP
69
101.243
56.700
54.579
1.00
44.34
C


ATOM
3816
CA
ASP
69
100.880
56.482
55.974
1.00
40.80
C


ATOM
3817
CB
ASP
69
100.101
57.683
56.515
1.00
56.37
C


ATOM
3818
CG
ASP
69
100.821
59.001
56.287
1.00
59.33
C


ATOM
3819
OD1
ASP
69
100.303
59.840
55.520
1.00
66.37
C


ATOM
3820
OD2
ASP
69
101.902
59.200
56.877
1.00
68.80
C


ATOM
3821
C
ASP
69
100.001
55.240
56.013
1.00
32.24
C


ATOM
3822
O
ASP
69
99.172
55.051
55.128
1.00
33.63
C


ATOM
3823
N
GLY
70
100.175
54.395
57.026
1.00
34.87
C


ATOM
3824
CA
GLY
70
99.369
53.184
57.107
1.00
28.23
C


ATOM
3825
C
GLY
70
98.691
52.933
58.444
1.00
28.75
C


ATOM
3826
O
GLY
70
99.260
53.198
59.501
1.00
34.76
C


ATOM
3827
N
LYS
71
97.468
52.416
58.394
1.00
40.41
C


ATOM
3828
CA
LYS
71
96.697
52.123
59.599
1.00
49.78
C


ATOM
3829
CB
LYS
71
95.522
53.095
59.714
1.00
57.09
C


ATOM
3830
CG
LYS
71
95.747
54.285
60.629
1.00
56.31
C


ATOM
3831
CD
LYS
71
94.477
55.139
60.720
1.00
55.32
C


ATOM
3832
CE
LYS
71
94.459
56.004
61.984
1.00
59.27
C


ATOM
3833
NZ
LYS
71
93.094
56.536
62.310
1.00
33.49
C


ATOM
3834
C
LYS
71
96.160
50.690
59.553
1.00
51.01
C


ATOM
3835
O
LYS
71
95.553
50.274
58.568
1.00
42.24
C


ATOM
3836
N
LEU
72
96.373
49.940
60.628
1.00
51.79
C


ATOM
3837
CA
LEU
72
95.907
48.564
60.684
1.00
61.74
C


ATOM
3838
CB
LEU
72
96.773
47.760
61.650
1.00
62.34
C


ATOM
3839
CG
LEU
72
97.284
46.425
61.103
1.00
66.36
C


ATOM
3840
CD1
LEU
72
98.165
45.754
62.147
1.00
68.74
C


ATOM
3841
CD2
LEU
72
96.107
45.527
60.736
1.00
53.65
C


ATOM
3842
C
LEU
72
94.436
48.447
61.090
1.00
73.73
C


ATOM
3843
O
LEU
72
93.860
49.374
61.663
1.00
88.59
C


ATOM
3844
N
GLY
73
93.835
47.298
60.789
1.00
67.09
C


ATOM
3845
CA
GLY
73
92.443
47.075
61.126
1.00
50.60
C


ATOM
3846
C
GLY
73
91.964
45.670
60.808
1.00
56.73
C


ATOM
3847
O
GLY
73
91.356
45.434
59.760
1.00
64.99
C


ATOM
3848
N
ASN
74
92.246
44.735
61.713
1.00
46.04
C


ATOM
3849
CA
ASN
74
91.828
43.342
61.566
1.00
54.16
C


ATOM
3850
CB
ASN
74
90.316
43.231
61.790
1.00
79.65
C


ATOM
3851
CG
ASN
74
89.953
42.174
62.814
1.00
90.87
C


ATOM
3852
OD1
ASN
74
88.992
42.334
63.574
1.00
90.68
C


ATOM
3853
ND2
ASN
74
90.715
41.086
62.838
1.00
89.35
C


ATOM
3854
C
ASN
74
92.180
42.738
60.215
1.00
54.40
C


ATOM
3855
O
ASN
74
93.332
42.413
59.951
1.00
62.07
C


ATOM
3856
N
GLU
75
91.171
42.577
59.364
1.00
55.88
C


ATOM
3857
CA
GLU
75
91.364
42.010
58.035
1.00
47.09
C


ATOM
3858
CB
GLU
75
90.269
40.988
57.722
1.00
56.56
C


ATOM
3859
CG
GLU
75
88.966
41.211
58.471
1.00
48.68
C


ATOM
3860
CD
GLU
75
88.614
40.047
59.379
1.00
58.55
C


ATOM
3861
OE1
GLU
75
88.407
38.928
58.859
1.00
55.16
C


ATOM
3862
OE2
GLU
75
88.547
40.250
60.612
1.00
54.08
C


ATOM
3863
C
GLU
75
91.323
43.127
57.013
1.00
44.01
C


ATOM
3864
O
GLU
75
90.606
43.055
56.017
1.00
27.15
C


ATOM
3865
N
SER
76
92.107
44.162
57.268
1.00
36.46
C


ATOM
3866
CA
SER
76
92.143
45.296
56.376
1.00
31.85
C


ATOM
3867
CB
SER
76
90.835
46.072
56.492
1.00
24.92
C


ATOM
3868
OG
SER
76
90.835
46.835
57.681
1.00
16.45
C


ATOM
3869
C
SER
76
93.292
46.208
56.733
1.00
32.47
C


ATOM
3870
O
SER
76
93.689
46.294
57.892
1.00
34.00
C


ATOM
3871
N
VAL
77
93.826
46.884
55.723
1.00
38.60
C


ATOM
3872
CA
VAL
77
94.906
47.848
55.908
1.00
32.38
C


ATOM
3873
CB
VAL
77
96.272
47.308
55.386
1.00
45.71
C


ATOM
3874
CG1
VAL
77
96.747
48.122
54.190
1.00
37.47
C


ATOM
3875
CG2
VAL
77
97.327
47.372
56.484
1.00
19.29
C


ATOM
3876
C
VAL
77
94.509
49.083
55.101
1.00
33.67
C


ATOM
3877
O
VAL
77
93.934
48.972
54.013
1.00
38.06
C


ATOM
3878
N
THR
78
94.811
50.260
55.630
1.00
40.03
C


ATOM
3879
CA
THR
78
94.475
51.493
54.929
1.00
38.24
C


ATOM
3880
CB
THR
78
93.548
52.377
55.787
1.00
35.10
C


ATOM
3881
OG1
THR
78
92.594
51.551
56.472
1.00
36.53
C


ATOM
3882
CG2
THR
78
92.804
53.363
54.916
1.00
36.45
C


ATOM
3883
C
THR
78
95.745
52.269
54.580
1.00
35.91
C


ATOM
3884
O
THR
78
96.574
52.540
55.451
1.00
31.92
C


ATOM
3885
N
PHE
79
95.896
52.610
53.301
1.00
39.13
C


ATOM
3886
CA
PHE
79
97.059
53.358
52.824
1.00
29.89
C


ATOM
3887
CB
PHE
79
97.532
52.801
51.487
1.00
35.47
C


ATOM
3888
CG
PHE
79
97.996
51.380
51.553
1.00
41.67
C


ATOM
3889
CD1
PHE
79
97.174
50.342
51.123
1.00
46.33
C


ATOM
3890
CD2
PHE
79
99.271
51.077
52.019
1.00
35.39
C


ATOM
3891
CE1
PHE
79
97.621
49.026
51.155
1.00
34.72
C


ATOM
3892
CE2
PHE
79
99.724
49.765
52.054
1.00
17.78
C


ATOM
3893
CZ
PHE
79
98.895
48.736
51.619
1.00
26.82
C


ATOM
3894
C
PHE
79
96.687
54.816
52.645
1.00
20.01
C


ATOM
3895
O
PHE
79
95.843
55.143
51.817
1.00
39.07
C


ATOM
3896
N
TYR
80
97.328
55.690
53.414
1.00
37.92
C


ATOM
3897
CA
TYR
80
97.048
57.123
53.361
1.00
39.66
C


ATOM
3898
CB
TYR
80
97.043
57.706
54.775
1.00
41.42
C


ATOM
3899
CG
TYR
80
96.541
59.125
54.849
1.00
29.87
C


ATOM
3900
CD1
TYR
80
95.857
59.702
53.782
1.00
31.82
C


ATOM
3901
CE1
TYR
80
95.404
61.008
53.842
1.00
38.26
C


ATOM
3902
CD2
TYR
80
96.756
59.895
55.984
1.00
35.48
C


ATOM
3903
CE2
TYR
80
96.307
61.207
56.060
1.00
38.78
C


ATOM
3904
CZ
TYR
80
95.632
61.758
54.984
1.00
46.52
C


ATOM
3905
OH
TYR
80
95.195
63.066
55.046
1.00
43.37
C


ATOM
3906
C
TYR
80
98.034
57.901
52.508
1.00
36.43
C


ATOM
3907
O
TYR
80
98.960
58.529
53.028
1.00
41.51
C


ATOM
3908
N
LEU
81
97.817
57.873
51.199
1.00
37.66
C


ATOM
3909
CA
LEU
81
98.694
58.576
50.268
1.00
43.35
C


ATOM
3910
CB
LEU
81
98.538
57.997
48.861
1.00
40.97
C


ATOM
3911
CG
LEU
81
98.392
56.485
48.721
1.00
35.20
C


ATOM
3912
CD1
LEU
81
98.068
56.154
47.268
1.00
41.59
C


ATOM
3913
CD2
LEU
81
99.670
55.798
49.141
1.00
34.03
C


ATOM
3914
C
LEU
81
98.457
60.092
50.216
1.00
39.41
C


ATOM
3915
O
LEU
81
97.410
60.552
49.759
1.00
30.90
C


ATOM
3916
N
GLN
82
99.450
60.853
50.678
1.00
45.56
C


ATOM
3917
CA
GLN
82
99.397
62.315
50.681
1.00
47.39
C


ATOM
3918
CB
GLN
82
99.884
62.868
52.023
1.00
48.95
C


ATOM
3919
CG
GLN
82
99.479
62.062
53.246
1.00
42.53
C


ATOM
3920
CD
GLN
82
99.397
62.921
54.491
1.00
35.76
C


ATOM
3921
OE1
GLN
82
99.826
62.514
55.575
1.00
35.27
C


ATOM
3922
NE2
GLN
82
98.857
64.127
54.339
1.00
29.92
C


ATOM
3923
C
GLN
82
100.280
62.884
49.571
1.00
42.88
C


ATOM
3924
O
GLN
82
101.148
62.194
49.035
1.00
41.58
C


ATOM
3925
N
ASN
83
100.060
64.148
49.237
1.00
45.46
C


ATOM
3926
CA
ASN
83
100.843
64.806
48.197
1.00
43.88
C


ATOM
3927
CB
ASN
83
102.229
65.139
48.748
1.00
38.56
C


ATOM
3928
CG
ASN
83
102.170
66.242
49.779
1.00
47.82
C


ATOM
3929
OD1
ASN
83
101.274
67.083
49.735
1.00
46.58
C


ATOM
3930
ND2
ASN
83
103.118
66.249
50.715
1.00
33.98
C


ATOM
3931
C
ASN
83
100.951
63.966
46.933
1.00
37.91
C


ATOM
3932
O
ASN
83
102.045
63.718
46.424
1.00
45.24
C


ATOM
3933
N
LEU
84
99.803
63.535
46.430
1.00
28.03
C


ATOM
3934
CA
LEU
84
99.760
62.723
45.230
1.00
19.39
C


ATOM
3935
CB
LEU
84
98.540
61.802
45.263
1.00
26.73
C


ATOM
3936
CG
LEU
84
98.774
60.341
45.664
1.00
34.93
C


ATOM
3937
CD1
LEU
84
97.483
59.552
45.502
1.00
37.57
C


ATOM
3938
CD2
LEU
84
99.877
59.729
44.812
1.00
36.66
C


ATOM
3939
C
LEU
84
99.675
63.650
44.037
1.00
20.07
C


ATOM
3940
O
LEU
84
98.914
64.609
44.049
1.00
31.39
C


ATOM
3941
N
TYR
85
100.460
63.365
43.006
1.00
33.90
C


ATOM
3942
CA
TYR
85
100.464
64.187
41.806
1.00
40.42
C


ATOM
3943
CB
TYR
85
101.840
64.117
41.123
1.00
59.23
C


ATOM
3944
CG
TYR
85
102.989
64.613
41.980
1.00
61.79
C


ATOM
3945
CD1
TYR
85
103.155
65.973
42.242
1.00
63.76
C


ATOM
3946
CE1
TYR
85
104.193
66.430
43.053
1.00
67.56
C


ATOM
3947
CD2
TYR
85
103.897
63.717
42.549
1.00
64.22
C


ATOM
3948
CE2
TYR
85
104.938
64.162
43.360
1.00
65.83
C


ATOM
3949
CZ
TYR
85
105.079
65.519
43.610
1.00
70.24
C


ATOM
3950
OH
TYR
85
106.093
65.965
44.428
1.00
73.68
C


ATOM
3951
C
TYR
85
99.378
63.724
40.841
1.00
33.25
C


ATOM
3952
O
TYR
85
98.880
62.606
40.943
1.00
33.16
C


ATOM
3953
N
VAL
86
99.018
64.598
39.907
1.00
32.80
C


ATOM
3954
CA
VAL
86
97.994
64.297
38.914
1.00
31.92
C


ATOM
3955
CB
VAL
86
97.503
65.600
38.241
1.00
29.33
C


ATOM
3956
CG1
VAL
86
98.476
66.730
38.548
1.00
32.20
C


ATOM
3957
CG2
VAL
86
97.361
65.409
36.743
1.00
34.84
C


ATOM
3958
C
VAL
86
98.504
63.312
37.854
1.00
30.06
C


ATOM
3959
O
VAL
86
97.721
62.621
37.204
1.00
28.12
C


ATOM
3960
N
ASN
87
99.819
63.246
37.685
1.00
37.39
C


ATOM
3961
CA
ASN
87
100.411
62.330
36.716
1.00
31.78
C


ATOM
3962
CB
ASN
87
101.803
62.811
36.348
1.00
30.37
C


ATOM
3963
CG
ASN
87
102.721
62.866
37.542
1.00
15.74
C


ATOM
3964
OD1
ASN
87
102.334
62.461
38.631
1.00
26.61
C


ATOM
3965
ND2
ASN
87
103.939
63.370
37.350
1.00
44.07
C


ATOM
3966
C
ASN
87
100.504
60.955
37.374
1.00
31.73
C


ATOM
3967
O
ASN
87
100.964
59.981
36.773
1.00
29.36
C


ATOM
3968
N
GLN
88
100.063
60.897
38.627
1.00
33.61
C


ATOM
3969
CA
GLN
88
100.082
59.665
39.402
1.00
24.73
C


ATOM
3970
CB
GLN
88
100.430
59.983
40.871
1.00
26.06
C


ATOM
3971
CG
GLN
88
101.721
59.314
41.400
1.00
44.43
C


ATOM
3972
CD
GLN
88
102.692
60.285
42.081
1.00
36.54
C


ATOM
3973
OE1
GLN
88
102.504
60.675
43.235
1.00
26.99
C


ATOM
3974
NE2
GLN
88
103.743
60.668
41.363
1.00
38.97
C


ATOM
3975
C
GLN
88
98.747
58.896
39.322
1.00
26.16
C


ATOM
3976
O
GLN
88
98.635
57.805
39.872
1.00
22.71
C


ATOM
3977
N
THR
89
97.735
59.448
38.645
1.00
29.74
C


ATOM
3978
CA
THR
89
96.450
58.742
38.548
1.00
35.51
C


ATOM
3979
CB
THR
89
95.381
59.548
37.786
1.00
23.56
C


ATOM
3980
OG1
THR
89
95.586
59.391
36.381
1.00
37.82
C


ATOM
3981
CG2
THR
89
95.456
61.008
38.140
1.00
8.56
C


ATOM
3982
C
THR
89
96.686
57.434
37.808
1.00
30.57
C


ATOM
3983
O
THR
89
97.222
57.427
36.701
1.00
19.99
C


ATOM
3984
N
ASP
90
96.274
56.326
38.411
1.00
26.02
C


ATOM
3985
CA
ASP
90
96.520
55.032
37.799
1.00
29.88
C


ATOM
3986
CB
ASP
90
98.023
54.758
37.848
1.00
17.27
C


ATOM
3987
CG
ASP
90
98.492
53.857
36.738
1.00
29.31
C


ATOM
3988
OD1
ASP
90
99.661
54.010
36.312
1.00
28.02
C


ATOM
3989
OD2
ASP
90
97.695
53.002
36.299
1.00
35.12
C


ATOM
3990
C
ASP
90
95.774
53.918
38.519
1.00
32.73
C


ATOM
3991
O
ASP
90
94.869
54.181
39.307
1.00
28.33
C


ATOM
3992
N
ILE
91
96.155
52.674
38.234
1.00
33.15
C


ATOM
3993
CA
ILE
91
95.549
51.524
38.891
1.00
29.34
C


ATOM
3994
CB
ILE
91
95.320
50.330
37.960
1.00
19.19
C


ATOM
3995
CG2
ILE
91
93.891
49.803
38.124
1.00
7.46
C


ATOM
3996
CG1
ILE
91
95.613
50.734
36.525
1.00
24.90
C


ATOM
3997
CD1
ILE
91
96.736
49.967
35.904
1.00
37.36
C


ATOM
3998
C
ILE
91
96.539
51.070
39.929
1.00
33.79
C


ATOM
3999
O
ILE
91
97.707
50.844
39.615
1.00
35.59
C


ATOM
4000
N
TYR
92
96.064
50.948
41.162
1.00
39.40
C


ATOM
4001
CA
TYR
92
96.891
50.521
42.274
1.00
25.36
C


ATOM
4002
CB
TYR
92
96.817
51.555
43.398
1.00
24.66
C


ATOM
4003
CG
TYR
92
97.472
52.872
43.039
1.00
27.16
C


ATOM
4004
CD1
TYR
92
98.646
53.282
43.669
1.00
32.14
C


ATOM
4005
CE1
TYR
92
99.265
54.474
43.327
1.00
24.99
C


ATOM
4006
CD2
TYR
92
96.933
53.697
42.051
1.00
24.44
C


ATOM
4007
CE2
TYR
92
97.547
54.896
41.703
1.00
24.44
C


ATOM
4008
CZ
TYR
92
98.712
55.275
42.344
1.00
18.97
C


ATOM
4009
OH
TYR
92
99.332
56.454
42.010
1.00
18.71
C


ATOM
4010
C
TYR
92
96.381
49.179
42.761
1.00
25.56
C


ATOM
4011
O
TYR
92
95.173
48.942
42.791
1.00
36.33
C


ATOM
4012
N
PHE
93
97.303
48.298
43.127
1.00
28.40
C


ATOM
4013
CA
PHE
93
96.931
46.984
43.621
1.00
29.94
C


ATOM
4014
CB
PHE
93
97.448
45.893
42.689
1.00
17.97
C


ATOM
4015
CG
PHE
93
97.105
46.113
41.258
1.00
24.43
C


ATOM
4016
CD1
PHE
93
97.978
46.793
40.422
1.00
27.44
C


ATOM
4017
CD2
PHE
93
95.921
45.617
40.733
1.00
30.96
C


ATOM
4018
CE1
PHE
93
97.673
46.973
39.076
1.00
46.97
C


ATOM
4019
CE2
PHE
93
95.610
45.792
39.391
1.00
31.89
C


ATOM
4020
CZ
PHE
93
96.483
46.467
38.562
1.00
33.13
C


ATOM
4021
C
PHE
93
97.470
46.739
45.020
1.00
35.26
C


ATOM
4022
O
PHE
93
98.635
47.022
45.325
1.00
34.73
C


ATOM
4023
N
CYS
94
96.601
46.209
45.870
1.00
48.06
C


ATOM
4024
CA
CYS
94
96.969
45.907
47.235
1.00
36.83
C


ATOM
4025
C
CYS
94
97.635
44.546
47.284
1.00
31.31
C


ATOM
4026
O
CYS
94
97.397
43.692
46.431
1.00
33.02
C


ATOM
4027
CB
CYS
94
95.738
45.921
48.121
1.00
39.16
C


ATOM
4028
SG
CYS
94
96.102
46.711
49.706
1.00
59.22
C


ATOM
4029
N
LYS
95
98.475
44.349
48.288
1.00
35.15
C


ATOM
4030
CA
LYS
95
99.194
43.093
48.430
1.00
32.02
C


ATOM
4031
CB
LYS
95
100.593
43.227
47.824
1.00
20.95
C


ATOM
4032
CG
LYS
95
101.265
41.913
47.532
1.00
16.51
C


ATOM
4033
CD
LYS
95
102.720
42.111
47.179
1.00
20.20
C


ATOM
4034
CE
LYS
95
103.419
40.772
46.979
1.00
39.20
C


ATOM
4035
NZ
LYS
95
104.812
40.931
46.471
1.00
47.09
C


ATOM
4036
C
LYS
95
99.301
42.731
49.896
1.00
29.30
C


ATOM
4037
O
LYS
95
99.224
43.602
50.759
1.00
32.79
C


ATOM
4038
N
ILE
96
99.480
41.444
50.172
1.00
24.46
C


ATOM
4039
CA
ILE
96
99.619
40.978
51.542
1.00
19.72
C


ATOM
4040
CB
ILE
96
98.258
40.925
52.251
1.00
14.76
C


ATOM
4041
CG2
ILE
96
97.299
40.052
51.477
1.00
17.83
C


ATOM
4042
CG1
ILE
96
98.434
40.405
53.673
1.00
17.25
C


ATOM
4043
CD1
ILE
96
99.184
41.352
54.570
1.00
5.02
C


ATOM
4044
C
ILE
96
100.276
39.599
51.602
1.00
26.57
C


ATOM
4045
O
ILE
96
99.852
38.662
50.916
1.00
34.37
C


ATOM
4046
N
GLU
97
101.321
39.491
52.419
1.00
20.47
C


ATOM
4047
CA
GLU
97
102.052
38.243
52.592
1.00
27.23
C


ATOM
4048
CB
GLU
97
103.367
38.279
51.793
1.00
32.57
C


ATOM
4049
CG
GLU
97
104.186
39.550
51.949
1.00
38.60
C


ATOM
4050
CD
GLU
97
104.535
40.193
50.618
1.00
46.08
C


ATOM
4051
OE1
GLU
97
105.239
39.548
49.815
1.00
37.73
C


ATOM
4052
OE2
GLU
97
104.108
41.348
50.379
1.00
54.19
C


ATOM
4053
C
GLU
97
102.343
37.951
54.071
1.00
30.22
C


ATOM
4054
O
GLU
97
102.705
38.841
54.844
1.00
27.30
C


ATOM
4055
N
VAL
98
102.158
36.694
54.455
1.00
24.37
C


ATOM
4056
CA
VAL
98
102.395
36.256
55.817
1.00
17.97
C


ATOM
4057
CB
VAL
98
101.361
35.183
56.224
1.00
33.66
C


ATOM
4058
CG1
VAL
98
102.002
34.115
57.113
1.00
46.61
C


ATOM
4059
CG2
VAL
98
100.200
35.844
56.931
1.00
31.46
C


ATOM
4060
C
VAL
98
103.787
35.664
55.808
1.00
24.36
C


ATOM
4061
O
VAL
98
104.147
34.979
54.858
1.00
17.44
C


ATOM
4062
N
MET
99
104.569
35.936
56.851
1.00
41.56
C


ATOM
4063
CA
MET
99
105.932
35.414
56.940
1.00
37.25
C


ATOM
4064
CB
MET
99
106.951
36.540
56.753
1.00
26.44
C


ATOM
4065
CG
MET
99
106.449
37.906
57.176
1.00
37.71
C


ATOM
4066
SD
MET
99
106.981
39.212
56.039
1.00
49.54
C


ATOM
4067
CE
MET
99
105.587
39.271
54.899
1.00
38.22
C


ATOM
4068
C
MET
99
106.182
34.728
58.273
1.00
37.57
C


ATOM
4069
O
MET
99
107.286
34.241
58.526
1.00
31.40
C


ATOM
4070
N
TYR
100
105.154
34.712
59.119
1.00
45.50
C


ATOM
4071
CA
TYR
100
105.210
34.084
60.439
1.00
45.47
C


ATOM
4072
CB
TYR
100
106.441
34.532
61.218
1.00
49.87
C


ATOM
4073
CG
TYR
100
106.979
33.489
62.176
1.00
54.53
C


ATOM
4074
CD1
TYR
100
107.908
32.545
61.745
1.00
66.10
C


ATOM
4075
CE1
TYR
100
108.466
31.625
62.622
1.00
67.96
C


ATOM
4076
CD2
TYR
100
106.611
33.483
63.523
1.00
50.84
C


ATOM
4077
CE2
TYR
100
107.166
32.559
64.415
1.00
57.94
C


ATOM
4078
CZ
TYR
100
108.098
31.636
63.951
1.00
57.97
C


ATOM
4079
OH
TYR
100
108.687
30.728
64.801
1.00
53.54
C


ATOM
4080
C
TYR
100
103.978
34.429
61.259
1.00
49.53
C


ATOM
4081
O
TYR
100
103.561
35.587
61.307
1.00
42.87
C


ATOM
4082
N
PRO
101
103.380
33.422
61.920
1.00
53.02
C


ATOM
4083
CD
PRO
101
102.180
33.528
62.771
1.00
52.41
C


ATOM
4084
CA
PRO
101
103.902
32.054
61.844
1.00
46.66
C


ATOM
4085
CB
PRO
101
103.126
31.300
62.927
1.00
42.16
C


ATOM
4086
CG
PRO
101
101.906
32.115
63.189
1.00
38.19
C


ATOM
4087
C
PRO
101
103.711
31.468
60.441
1.00
57.32
C


ATOM
4088
O
PRO
101
102.901
31.960
59.648
1.00
47.74
C


ATOM
4089
N
PRO
102
104.455
30.400
60.122
1.00
58.81
C


ATOM
4090
CD
PRO
102
105.410
29.707
61.000
1.00
43.99
C


ATOM
4091
CA
PRO
102
104.368
29.758
58.807
1.00
54.29
C


ATOM
4092
CB
PRO
102
105.585
28.824
58.766
1.00
46.96
C


ATOM
4093
CG
PRO
102
106.354
29.090
60.030
1.00
46.28
C


ATOM
4094
C
PRO
102
103.072
28.997
58.565
1.00
50.83
C


ATOM
4095
O
PRO
102
102.265
28.812
59.475
1.00
51.79
C


ATOM
4096
N
PRO
103
102.858
28.550
57.321
1.00
43.58
C


ATOM
4097
CD
PRO
103
101.692
27.757
56.901
1.00
38.84
C


ATOM
4098
CA
PRO
103
103.784
28.756
56.209
1.00
34.80
C


ATOM
4099
CB
PRO
103
103.457
27.611
55.247
1.00
35.84
C


ATOM
4100
CG
PRO
103
102.280
26.863
55.860
1.00
31.11
C


ATOM
4101
C
PRO
103
103.581
30.127
55.566
1.00
39.20
C


ATOM
4102
O
PRO
103
102.790
30.939
56.055
1.00
35.30
C


ATOM
4103
N
TYR
104
104.311
30.382
54.481
1.00
40.51
C


ATOM
4104
CA
TYR
104
104.199
31.644
53.760
1.00
40.50
C


ATOM
4105
CB
TYR
104
105.226
31.719
52.630
1.00
34.12
C


ATOM
4106
CG
TYR
104
105.224
33.036
51.888
1.00
35.99
C


ATOM
4107
CD1
TYR
104
104.659
33.143
50.615
1.00
49.18
C


ATOM
4108
CE1
TYR
104
104.652
34.363
49.926
1.00
50.34
C


ATOM
4109
CD2
TYR
104
105.784
34.179
52.458
1.00
34.98
C


ATOM
4110
CE2
TYR
104
105.782
35.403
51.779
1.00
35.62
C


ATOM
4111
CZ
TYR
104
105.215
35.485
50.518
1.00
39.99
C


ATOM
4112
OH
TYR
104
105.205
36.685
49.853
1.00
32.85
C


ATOM
4113
C
TYR
104
102.804
31.725
53.168
1.00
44.36
C


ATOM
4114
O
TYR
104
102.311
30.749
52.603
1.00
46.76
C


ATOM
4115
N
LEU
105
102.171
32.885
53.304
1.00
47.99
C


ATOM
4116
CA
LEU
105
100.827
33.096
52.778
1.00
43.06
C


ATOM
4117
CB
LEU
105
99.859
33.347
53.929
1.00
44.75
C


ATOM
4118
CG
LEU
105
98.748
32.310
54.097
1.00
55.87
C


ATOM
4119
CD1
LEU
105
98.181
31.958
52.725
1.00
60.46
C


ATOM
4120
CD2
LEU
105
99.289
31.062
54.797
1.00
56.52
C


ATOM
4121
C
LEU
105
100.819
34.283
51.813
1.00
45.31
C


ATOM
4122
O
LEU
105
101.438
35.308
52.085
1.00
44.10
C


ATOM
4123
N
ASP
106
100.107
34.144
50.696
1.00
53.21
C


ATOM
4124
CA
ASP
106
100.046
35.199
49.681
1.00
56.65
C


ATOM
4125
CB
ASP
106
100.961
34.827
48.512
1.00
73.34
C


ATOM
4126
CG
ASP
106
101.724
36.011
47.979
1.00
81.75
C


ATOM
4127
OD1
ASP
106
101.437
37.143
48.432
1.00
85.62
C


ATOM
4128
OD2
ASP
106
102.603
35.802
47.109
1.00
80.61
C


ATOM
4129
C
ASP
106
98.639
35.462
49.145
1.00
52.61
C


ATOM
4130
O
ASP
106
97.772
34.592
49.199
1.00
62.59
C


ATOM
4131
N
ASN
107
98.422
36.658
48.609
1.00
42.29
C


ATOM
4132
CA
ASN
107
97.110
37.028
48.072
1.00
50.80
C


ATOM
4133
CB
ASN
107
96.826
38.492
48.359
1.00
42.50
C


ATOM
4134
CG
ASN
107
97.855
39.393
47.736
1.00
41.45
C


ATOM
4135
OD1
ASN
107
97.691
39.852
46.607
1.00
44.69
C


ATOM
4136
ND2
ASN
107
98.940
39.637
48.461
1.00
38.52
C


ATOM
4137
C
ASN
107
96.997
36.815
46.564
1.00
55.83
C


ATOM
4138
O
ASN
107
97.990
36.865
45.840
1.00
54.74
C


ATOM
4139
N
GLU
108
95.771
36.601
46.098
1.00
61.82
C


ATOM
4140
CA
GLU
108
95.513
36.393
44.678
1.00
62.32
C


ATOM
4141
CB
GLU
108
94.305
35.474
44.485
1.00
65.84
C


ATOM
4142
CG
GLU
108
93.817
34.809
45.757
1.00
72.69
C


ATOM
4143
CD
GLU
108
92.445
35.295
46.180
1.00
73.87
C


ATOM
4144
OE1
GLU
108
91.534
34.453
46.327
1.00
58.56
C


ATOM
4145
OE2
GLU
108
92.285
36.522
46.366
1.00
79.05
C


ATOM
4146
C
GLU
108
95.244
37.722
43.991
1.00
64.40
C


ATOM
4147
O
GLU
108
94.094
38.171
43.936
1.00
61.70
C


ATOM
4148
N
LYS
109
96.306
38.339
43.467
1.00
64.63
C


ATOM
4149
CA
LYS
109
96.215
39.629
42.782
1.00
60.84
C


ATOM
4150
CB
LYS
109
96.054
39.420
41.274
1.00
43.96
C


ATOM
4151
CG
LYS
109
97.094
38.496
40.666
1.00
66.29
C


ATOM
4152
CD
LYS
109
98.399
39.235
40.373
1.00
84.02
C


ATOM
4153
CE
LYS
109
99.309
38.435
39.432
1.00
77.45
C


ATOM
4154
NZ
LYS
109
100.234
39.299
38.636
1.00
53.39
C


ATOM
4155
C
LYS
109
95.040
40.435
43.331
1.00
59.47
C


ATOM
4156
O
LYS
109
94.893
40.571
44.545
1.00
73.09
C


ATOM
4157
N
SER
110
94.201
40.950
42.440
1.00
49.53
C


ATOM
4158
CA
SER
110
93.038
41.734
42.841
1.00
50.60
C


ATOM
4159
CB
SER
110
93.404
42.706
43.969
1.00
48.38
C


ATOM
4160
OG
SER
110
93.807
43.962
43.448
1.00
55.94
C


ATOM
4161
C
SER
110
92.455
42.520
41.670
1.00
49.16
C


ATOM
4162
O
SER
110
93.098
42.693
40.634
1.00
36.85
C


ATOM
4163
N
ASN
111
91.228
43.000
41.841
1.00
50.24
C


ATOM
4164
CA
ASN
111
90.580
43.770
40.795
1.00
48.26
C


ATOM
4165
CB
ASN
111
89.073
43.868
41.068
1.00
50.05
C


ATOM
4166
CG
ASN
111
88.273
42.770
40.364
1.00
52.55
C


ATOM
4167
OD1
ASN
111
87.101
42.539
40.671
1.00
30.90
C


ATOM
4168
ND2
ASN
111
88.912
42.089
39.413
1.00
60.36
C


ATOM
4169
C
ASN
111
91.212
45.162
40.708
1.00
42.47
C


ATOM
4170
O
ASN
111
90.982
45.896
39.748
1.00
48.01
C


ATOM
4171
N
GLY
112
92.007
45.517
41.717
1.00
44.01
C


ATOM
4172
CA
GLY
112
92.682
46.808
41.729
1.00
36.00
C


ATOM
4173
C
GLY
112
91.830
48.015
42.081
1.00
36.58
C


ATOM
4174
O
GLY
112
90.630
47.892
42.343
1.00
33.31
C


ATOM
4175
N
THR
113
92.455
49.189
42.094
1.00
28.16
C


ATOM
4176
CA
THR
113
91.738
50.416
42.403
1.00
29.29
C


ATOM
4177
CB
THR
113
91.671
50.667
43.929
1.00
25.84
C


ATOM
4178
OG1
THR
113
90.959
51.884
44.186
1.00
13.15
C


ATOM
4179
CG2
THR
113
93.061
50.762
44.514
1.00
35.81
C


ATOM
4180
C
THR
113
92.344
51.636
41.722
1.00
29.94
C


ATOM
4181
O
THR
113
93.375
52.157
42.133
1.00
21.21
C


ATOM
4182
N
ILE
114
91.679
52.074
40.662
1.00
31.79
C


ATOM
4183
CA
ILE
114
92.093
53.243
39.905
1.00
24.64
C


ATOM
4184
CB
ILE
114
91.132
53.521
38.721
1.00
29.78
C


ATOM
4185
CG2
ILE
114
91.159
55.001
38.361
1.00
29.89
C


ATOM
4186
CG1
ILE
114
91.510
52.661
37.519
1.00
45.18
C


ATOM
4187
CD1
ILE
114
91.376
53.376
36.182
1.00
48.49
C


ATOM
4188
C
ILE
114
92.019
54.450
40.821
1.00
21.71
C


ATOM
4189
O
ILE
114
91.050
54.632
41.546
1.00
23.04
C


ATOM
4190
N
ILE
115
93.028
55.296
40.770
1.00
22.63
C


ATOM
4191
CA
ILE
115
93.003
56.483
41.593
1.00
25.37
C


ATOM
4192
CB
ILE
115
94.147
56.461
42.585
1.00
19.98
C


ATOM
4193
CG2
ILE
115
94.249
57.798
43.307
1.00
15.47
C


ATOM
4194
CG1
ILE
115
93.925
55.312
43.558
1.00
5.93
C


ATOM
4195
CD1
ILE
115
95.089
55.086
44.508
1.00
25.93
C


ATOM
4196
C
ILE
115
93.104
57.712
40.701
1.00
36.88
C


ATOM
4197
O
ILE
115
94.147
57.984
40.112
1.00
39.31
C


ATOM
4198
N
HIS
116
92.003
58.445
40.604
1.00
36.64
C


ATOM
4199
CA
HIS
116
91.951
59.641
39.779
1.00
42.31
C


ATOM
4200
CB
HIS
116
90.530
59.854
39.250
1.00
55.50
C


ATOM
4201
CG
HIS
116
90.397
61.008
38.303
1.00
53.01
C


ATOM
4202
CD2
HIS
116
89.311
61.534
37.690
1.00
53.38
C


ATOM
4203
ND1
HIS
116
91.473
61.764
37.884
1.00
49.21
C


ATOM
4204
CE1
HIS
116
91.054
62.700
37.058
1.00
52.55
C


ATOM
4205
NE2
HIS
116
89.744
62.584
36.920
1.00
46.03
C


ATOM
4206
C
HIS
116
92.380
60.847
40.592
1.00
38.13
C


ATOM
4207
O
HIS
116
91.655
61.278
41.486
1.00
35.69
C


ATOM
4208
N
VAL
117
93.554
61.387
40.274
1.00
35.30
C


ATOM
4209
CA
VAL
117
94.078
62.550
40.980
1.00
36.81
C


ATOM
4210
CB
VAL
117
95.606
62.463
41.146
1.00
24.40
C


ATOM
4211
CG1
VAL
117
96.075
63.529
42.109
1.00
30.24
C


ATOM
4212
CG2
VAL
117
96.004
61.087
41.647
1.00
21.51
C


ATOM
4213
C
VAL
117
93.747
63.857
40.271
1.00
44.32
C


ATOM
4214
O
VAL
117
93.901
63.973
39.054
1.00
48.83
C


ATOM
4215
N
LYS
118
93.304
64.837
41.055
1.00
61.70
C


ATOM
4216
CA
LYS
118
92.926
66.160
40.555
1.00
65.79
C


ATOM
4217
CB
LYS
118
94.076
66.809
39.779
1.00
48.95
C


ATOM
4218
CG
LYS
118
94.137
68.329
39.934
1.00
51.90
C


ATOM
4219
CD
LYS
118
93.956
69.065
38.603
1.00
54.76
C


ATOM
4220
CE
LYS
118
94.163
70.575
38.754
1.00
49.49
C


ATOM
4221
NZ
LYS
118
95.564
71.003
38.435
1.00
45.55
C


ATOM
4222
C
LYS
118
91.693
66.107
39.664
1.00
78.13
C


ATOM
4223
O
LYS
118
91.566
67.006
38.807
1.00
88.53
C


ATOM
4224
OT
LYS
118
90.872
65.178
39.832
1.00
83.59
C


END

















TABLE 5







DNA sequence of human CD28 cDNA (SEQ ID NO: 15)










agactctcag gccttggcag gtgcgtcttt cagttcccct cacacttcgg gttcctcggg
60



 


gaggaggggc tggaacccta gcccatcgtc aggacaaaga tgctcaggct gctcttggct
120


 


ctcaacttat tcccttcaat tcaagtaaca ggaaacaaga ttttggtgaa gcagtcgccc
180


 


atgcttgtag cgtacgacaa tgcggtcaac cttagctgca agtattccta caatctcttc
240


 


tcaagggagt tccgggcatc ccttcacaaa ggactggata gtgctgtgga agtctgtgtt
300


 


gtatatggga attactccca gcagcttcag gtttactcaa aaacggggtt caactgtgat
360


 


gggaaattgg gcaatgaatc agtgacattc tacctccaga atttgtatgt taaccaaaca
420


 


gatatttact tctgcaaaat tgaagttatg tatcctcctc cttacctaga caatgagaag
480


 


agcaatggaa ccattatcca tgtgaaaggg aaacaccttt gtccaagtcc cctatttccc
540


 


ggaccttcta agcccttttg ggtgctggtg gtggttggtg gagtcctggc ttgctatagc
600


 


ttgctagtaa cagtggcctt tattatttc tgggtcgagga gtaagaggag caggctcctg
660


 


cacagtgact acatgaacat gactccccgc cgccccgggc ccacccgcaa gcattaccag
720


 


ccctatgccc caccacgcga cttcgcagcc tatcgctcct gacacggacg cctatccaga
780


 


agccagccgg ctggcagccc ccatctgctc aatatcactg ctctggatag gaaatgaccg
840


 


ccatctccag ccggccacct cagcccctgt tgggccacca atgccaattt ttctcgagtg
900


 


actagaccaa atatcaagat cattttgaga ctctgaaatg aagtaaaaga gatttcctgt
960


 


gacaggccaa gtcttacagt gccatggccc acattccaac ttaccatgta cttagtgact
1020


 


tgactgagaa gttagggtag aaaacaaaaa gggagtggat tctgggagcc tcttcccttt
1080


 


ctcactcacc tgcacatctc agtcaagcaa agtgtggtat ccacagacat tttagttgca
1140


 


gaagaaaggc taggaaatca ttccttttgg ttaaatgggt gtttaatctt ttggttagtg
1200


 


ggttaaacgg ggtaagttag agtaggggga gggataggaa gacatattta aaaaccatta
1260


 


aaacactgtc tcccactcat gaaatgagcc acgtagttcc tatttaatgc tgttttcctt
1320


 


tagtttagaa atacatagac attgtctttt atgaattctg atcatattta gtcattttga
1380


 


ccaaatgagg gatttggtca aatgagggat tccctcaaag caatatcagg taaaccaagt
1440


 


tgctttcctc actccctgtc atgagacttc agtgttaatg ttcacaatat actttcgaaa
1500


 


gaataaaata gttc
1514


 


Amino acid sequence of human CD28 (SEQ ID NO: 1)








(SEQ ID NO:1)









MLRLLLALNL FPSIQVTGNK ILVKQSPMLV AYDNAVNLSC KYSYNLFSRE FRASLHKGLD



 


SAVEVCVVYG NYSQQLQVYS KTGFNCDGKL GNESVTFYLQ NLYVNQTDIY FCKIEVMYPP


 


PYLDNEKSNG TIIHVKGKHL CPSPLFPGPS KPFWVLVVVG GVLACYSLLV TVAFIIFWVR


 


SKRSRLLHSD YMNMTPRRPG PTRKHYQPYA PPRDFAAYRS





The extracellular domain is shown in bold


The stalk region is underlined














TABLE 6





CD28TFc sequence (SEQ ID NO:2)



(thrombin site separating the two halves of the chimera


is shown in bold)





















CCCCATCCGCTCAAGCAGGCCACCATGGATTGGCTGCGGAACTTGCTATTCCTGATGGCG





1
---------+---------+---------+---------+---------+---------+
60




GGGGTAGGCGAGTTCGTCCGGTGGTACCTAACCGACGCCTTGAACGATAAGGACTACCGC



a

                        M  D  W  L  R  N  L  L  F  L  M  A


 




GCCGCTCAAAGTATCAACGCGAACAAGATCTTGGTGAAGCAGTCGCCCATGCTTGTAGCG



61
---------+---------+---------+---------+---------+---------+
120




CGGCGAGTTTCATAGTTGCGCTTGTTCTAGAACCACTTCGTCAGCGGGTACGAACATCGC


a

A  A  Q  S  I  N  A  N  K  I  L  V  K  Q  S  P  M  L  V  A


 




TACGACAATGCGGTCAACCTTAGCTGCAAGTATTCCTACAATCTCTTCTCAAGGGAGTTC



121
---------+---------+---------+---------+---------+---------+
180




ATGCTGTTACGCCAGTTGGAATCGACGTTCATAAGGATGTTAGAGAAGAGTTCCCTCAAG


a

Y  D  N  A  V  N  L  S  C  K  Y  S  Y  N  L  F  S  R  E  F


 




CGGGCATCCCTTCACAAAGGACTGGATAGTGCTGTGGAAGTCTGTGTTGTATATGGGAAT



181
---------+---------+---------+---------+---------+---------+
240




GCCCGTAGGGAAGTGTTTCCTGACCTATCACGACACCTTCAGACACAACATATACCCTTA


a

R  A  S  L  H  K  G  L  D  S  A  V  E  V  C  V  V  Y  G  N


 




TACTCCCAGCAGCTTCAGGTTTACTCAAAAACGGGGTTCAACTGTGATGGGAAATTGGGC



241
---------+---------+---------+---------+---------+---------+
300


 




ATGAGGGTCGTCGAAGTCCAAATGAGTTTTTGCCCCAAGTTGACACTACCCTTTAACCCG


a

Y  S  Q  Q  L  Q  V  Y  S  K  T  G  F  N  C  D  G  K  L  G


 




AATGAATCAGTGACATTCTACCTCCAGAATTTGTATGTTAACCAAACAGATATTTACTTC



301
---------+---------+---------+---------+---------+---------+
360




TTACTTAGTCACTGTAAGATGGAGGTCTTAAACATACAATTGGTTTGTCTATAAATGAAG


a

N  E  S  V  T  F  Y  L  Q  N  L  Y  V  N  Q  T  D  I  Y  F


 




TGCAAAATTGAAGTTATGTATCCTCCTCCTTACCTAGACAATGAGAAGAGCAATGGTACC



361
---------+---------+---------+---------+---------+---------+
420




ACGTTTTAACTTCAATACATAGGAGGAGGAATGGATCTGTTACTCTTCTCGTTACCATGG


a

C  K  I  E  V  M  Y  P  P  P  Y  L  D  N  E  K  S  N  G  T


 




ATTATCCATGTGAAAGGGAAACACCTTTGTCCAAGTCCGCTATTTCCCGGACCTTCTAAG



421
---------+---------+---------+---------+---------+---------+
480




TAATAGGTACACTTTCCCTTTGTGGAAACAGGTTCAGGCGATAAAGGGCCTGGAAGATTC


a

I  I  H  V  K  G  K  H  L  C  P  S  P  L  F  P  G  P  S  K


 




CCCCTGGTACCCAGGGGTAGTGGTAGTAAGCCTAGCATAAGTACAGTCCCAGAAGTATCA



481
---------+---------+---------+---------+---------+---------+
540




GGGGACCATGGGTCCCCATCACCATCATTCGGATCGTATTCATGTCAGGGTCTTCATAGT


a

P  L  V  P  R  G  S  G  S  K  P  S  I  S  T  V  P  E  V  S


 




TCTGTCTTCATCTTCCCCCCAAAGCCCAAGGATGTGCTCACCATTACTCTGACTCCTAAG



541
---------+---------+---------+---------+---------+---------+
600




AGACAGAAGTAGAAGGGGGGTTTCGGGTTCCTACACGAGTGGTAATGAGACTGAGGATTC


a

S  V  F  I  F  P  P  K  P  K  D  V  L  T  I  T  L  T  P  K


 




GTCACGTGTGTTGTGGTAGACATCAGCAAGGATGATCCCGAGGTCCAGTTCAGCTGGTTT



601
---------+---------+---------+---------+---------+---------+
660




CAGTGCACACAACACCATCTGTAGTCGTTCCTACTAGGGCTCCAGGTCAAGTCGACCAAA


a

V  T  C  V  V  V  D  I  S  K  D  D  P  E  V  Q  F  S  W  F


 




GTAGATGATGTGGAGGTGCACACAGCTCAGACGCAACCCCGGGAGGAGCAGTTCAACAGC



661
---------+---------+---------+---------+---------+---------+
720




CATCTACTACACCTCCACGTGTGTCGAGTCTGCGTTGGGGCCCTCCTCGTCAAGTTGTCG


a

V  D  D  V  E  V  H  T  A  Q  T  Q  P  R  E  E  Q  F  N  S


 




ACTTTCCGCTCAGTCAGTGAACTTCCCATCATGCACCAGGACTGGCTCAATGGCAAGGAG



721
---------+---------+---------+---------+---------+---------+
780




TGAAAGGCGAGTCAGTCACTTGAAGGGTAGTACGTGGTCCTGACCGAGTTACCGTTCCTC


a

T  F  R  S  V  S  E  L  P  I  M  H  Q  D  W  L  N  G  K  E


 




TTCAAATGCAGGGTCAACAGTGCAGCTTTCCCTGCCCCCATCGAGAAAACCATCTCCAAA



781
---------+---------+---------+---------+---------+---------+
840




AAGTTTACGTCCCAGTTGTCACGTCGAAAGGGACGGGGGTAGCTCTTTTGGTAGAGGTTT


a

F  K  C  R  V  N  S  A  A  F  P  A  P  I  E  K  T  I  S  K


 




ACCAAAGGCAGACCGAAGGCTCCACAGGTGTACACCATTCCACCTCCCAAGGAGCAGATG



841
---------+---------+---------+---------+---------+---------+
900




TGGTTTCCGTCTGGCTTCCGAGGTGTCCACATGTGGTAAGGTGGAGGGTTCCTCGTCTAC


a

T  K  G  R  P  K  A  P  Q  V  Y  T  I  P  P  P  K  E  Q  M


 




GCCAAGGATAAAGTCAGTCTGACCTGCATGATAACAGACTTCTTCCCTGAAGACATTACT



901
---------+---------+---------+---------+---------+---------+
960




CGGTTCCTATTTCAGTCAGACTGGACGTACTATTGTCTGAAGAAGGGACTTCTGTAATGA


a

A  K  D  K  V  S  L  T  C  M  I  T  D  F  F  P  E  D  I  T


 




GTGGAGTGGCAGTGGAATGGGCAGCCAGCGGAGAACTACAAGAACACTCAGCCCATCATG



961
---------+---------+---------+---------+---------+---------+
1020




CACCTCACCGTCACCTTACCCGTCGGTCGCCTCTTGATGTTCTTGTGAGTCGGGTAGTAC


a

V  E  W  Q  W  N  G  Q  P  A  E  N  Y  K  N  T  Q  P  I  M


 




GACACAGATGGCTCTTACTTCGTCTACAGCAAGCTCAATGTGCAGAAGAGCAACTGGGAG



1021
---------+---------+---------+---------+---------+---------+
1080




CTGTGTCTACCGAGAATGAAGCAGATGTCGTTCGAGTTACACGTCTTCTCGTTGACCCTC


a

D  T  D  G  S  Y  F  V  Y  S  K  L  N  V  Q  K  S  N  W  E


 




GCAGGAAATACTTTCACCTGCTCTGTGTTACATGAGGGCCTGCACAACCACCATACTGAG



1081
---------+---------+---------+---------+---------+---------+
1140




CGTCCTTTATGAAAGTGGACGAGACACAATGTACTCCCGGACGTGTTGGTGGTATGACTC


a

A  G  N  T  F  T  C  S  V  L  H  E  G  L  H  N  H  H  T  E


 




AAGAGCCTCTCCCACTCTCCTGGTAAATAA



1141
---------+---------+---------+
1170




TTCTCGGAGAGGGTGAGAGGACCATTTATT


a

K  S  L  S  H  S  P  G  K  *

## Claims

1. An antibody that binds to the extracellular portion of the human PD-1 receptor at a membrane proximal region and said antibody binds to an epitope comprising SEQ ID NO: 44, SEQ ID NO: 46, SEQ ID NO: 48, SEQ ID NO: 50, SEQ ID NO: 52, SEQ ID NO: 54, SEQ ID NO: 56 or SEQ ID NO: 61, wherein:
a) said antibody does not only bind to a peptide consisting of LAAFPEDRSQPGQDCR (SEQ ID NO: 61), the human PD-1 equivalent of the C′-D loop of human CD28; and
b) said antibody induces superagonistic signaling of the PD-1 receptor by binding to a membrane proximal region of the human PD-1 receptor that is within 75 Å of a cell surface or wherein said antibody binds to a membrane proximal region of human PD-1 expressed on the surface of a cell and holds the cell surface within 200 Å of a substrate to which said antibody is immobilized.

2. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 44.

3. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 46.

4. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 48.

5. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 50.

6. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 52.

7. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 54.

8. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 56.

9. The antibody according to claim 1, wherein said antibody binds to an epitope comprising SEQ ID NO: 61.

10. The antibody according to claim 1, wherein said antibody is a monoclonal antibody.

11. The antibody according to claim 10, wherein said antibody is a humanized antibody.

12. The antibody according to claim 10, wherein said antibody is a complementarity determining region (CDR)-grafted antibody.

13. The antibody according to claim 10, wherein said antibody is a chimeric antibody.

14. The antibody according to claim 1, wherein said antibody induces superagonistic signaling of the PD-1 receptor by binding to a membrane proximal region of the human PD-1 receptor that is within 60 Å of a cell surface.

15. The antibody according to claim 1, wherein said antibody induces superagonistic signaling of the PD-1 receptor by binding to a membrane proximal region of the human PD-1 receptor that is within 50 Å of a cell surface.

16. The antibody according to claim 1, wherein said antibody induces superagonistic signaling of the PD-1 receptor by binding to a membrane proximal region of the human PD-1 receptor that is within 40 Å of a cell surface.

17. The antibody according to claim 1, wherein said antibody binds to a membrane proximal region of human PD-1 expressed on the surface of a cell and holds the cell surface within 180 Å of a substrate to which said antibody is immobilized.

18. The antibody according to claim 1, wherein said antibody hinds to a membrane proximal region of human PD-1 expressed on the surface of a cell and holds the cell surface within 150 Å of a substrate to which said antibody is immobilized.

19. The antibody according to claim 1, wherein said antibody binds to a membrane proximal region of human PD-1 expressed on the surface of a cell and holds the cell surface within 120 Å of a substrate to which said antibody is immobilized.