www.hydrozonix.com Recycle..Reuse..Results.. 

# Examining(Techniques(and(Chemistries( For(Managing(Bacteria,(Scaling(And( Corrosion(www.hydrozonix.com Recycle..Reuse..Results.. 

# Agenda(



# • Quan7fying.bacteria.

– Accepted.methods.

– Rapid.detec7on.methods. 

# • Tube>blocking.test.

– What.is.it?.

– How.is.it.used?. 

# • Hydrozonix.case.studies.

2. 

# Scale( Bacteria(

Barium'Sulfate' Calcium'Carbonate'' SRB'on'le3'www.hydrozonix.com Recycle..Reuse..Results.. 

# • Types.of.sample.collec7on.methods.

– Planktonic

– Sessile. 

# • Enumera7on.

– Two.primary.methods. • Most.probable.number.method.(SM.9221).

– Units.=.most.probable.number.per.ml.

– 14.days.for.APB.

– 28.days.for.SRB. 

### • Filtra7on.method.(SM.9215).

– Units.=.colony.forming.units.per.ml.

– 2.days.but.not.as.accurate.

– Normally.show.lower.than.actual.numbers.



## – Understand.test.accuracy. 3. 

# Agenda( Predominant(Bacteria(Tes=ng(Methods(www.hydrozonix.com Recycle..Reuse..Results.. 

# Rapid(Bacteria(Detec=on( 

# • qPCR .–.quan7ta7ve.polymerase.chain. reac7on.

– Detects.and.quan7fies.specific.genes.of. bacteria.

– Expensive. 

# • FISH.–.fluorescence.in.situ.hybridiza7on.

– Chemical.fluorescent.added.to.filtered.sample.

– Bacteria.show.up.as.spots.that.are.counted. 

# • ATP.–.adenosine.triphosphate.

– Molecule.found.in.all.living.organisms.

– Test.uses.this.molecule.to.measure.bacteria. presence. 4.www.hydrozonix.com Recycle..Reuse..Results.. 5. 

# TubeBBlocking(Test( ! 

## Flow.fluid.through.1/16”.capillary.pipe. ! 

## Heat.to.reservoir.temperature. ! 

## Measure.flowing.pressure.at.constant.rate. ! 

## Pressure.increase.=.scale.deposi7on.in.pipe. ! 

## Used.to.determine.appropriate.scale. inhibitor.dosing.www.hydrozonix.com Recycle..Reuse..Results.. 

# Hydrozonix (EF80( On>the>Fly.Treatment. 80.Barrels.Per.Minute. No.Liquid.Chemicals.Used. Microbial.Control.and.Scale. Inhib7on

6.www.hydrozonix.com Recycle..Reuse..Results.. 

# EF80(on(a(Frac(in(West(Texas(

7.www.hydrozonix.com Recycle..Reuse..Results.. 

# Compe==on(Comparison(Matrix( Ozonix TM Chlorine(Dioxide( Liquid(Biocide( (e.g.,( glute / quat )( Liquid(Scale( Inhibitor((e.g.,(

phosponate )( Iron(Control( Agent((chela=ng( agent)( Compa=bility(w/(Other(Frac( Chemicals((Fric=on(Reducer(/( Linear(&(Crosslink(Gel)( FRICTION( REDUCER( GEL( Bacteria(Treatment( Scale(Inhibi=on( Iron(Control( Iron(Sulfide(Preven=on(/( Treatment( Hydrogen(Sulfide(Preven=on(/( Treatment( Significant(Favorable( Impact( Minimally(Favorable( Impact( Zero(or(Unfavorable( Impact( 8.www.hydrozonix.com Recycle..Reuse..Results.. 

### CASE.STUDY.DESCRIPTION. • Permian.Basin,.West.Texas. • Frac.fluid.treatment. • On>the>fly.treatment.at.80. bpm • Mul7>stage. slickwater frac • Rig.up:..trea7ng.80/20.fresh.water.flowback.mixture.

9. 

# Case(Study(1(–(Bacteria(Treatment(www.hydrozonix.com Recycle..Reuse..Results.. 

## Case(Study(1(–(Bacteria(Treatment

0. 50,000. 100,000. 150,000. 200,000. 250,000. 300,000.

3. 3. 4. 4. 5. 5. 6. 6. 7. 7. 8. 8. 9. 9. 10. 10. 11. 11.#(of(Cells(Per(Milliliter(

Stage(Number( 

### OnBtheBFly(Bacteria(Treatment(Results((11(Stages)( Influent. Effluent. 

# 99.98% REDUCTION

10.www.hydrozonix.com Recycle..Reuse..Results.. 

### CASE.STUDY.DESCRIPTION. • Marcellus.shale.flowback.water. • 30,000.mg/l.total.dissolved.solids. • Pit.treatment. • 60,000. bbl .treated.in.three.days. • Rig.up:..circula7ng.pit.

11. 

# Case(Study(2(–(Scale(Inhibi=on(www.hydrozonix.com Recycle..Reuse..Results.. >5.00.

0.00. 5.00. 10.00. 15.00. 20.00. 25.00. 30.00. 35.00. 40.00.

0.00. 1.00. 2.00. 3.00. 4.00. 5.00. 6.00. 7.00. 8.00. 9.00.Change(in(Differen=al(Pressure((PSIG)(

Time(in(Hours( 

### TubeBBlocking(Test(Results(–(A(Test(for(Scaling(Tendency( Effluent.>.Treated.Fluid. Influent.>.Raw.Fluid.

12. 

# Case(Study(2(–(Scale(Inhibi=on( 

# 96.40% REDUCTION In scale depositionwww.hydrozonix.com Recycle..Reuse..Results.. 13. 

# Case(Study(3(–(Fric=on(Reducer(Compa=bility( CASE.STUDY.DESCRIPTION. • Wolfcamp .produced.fluid. • 97,900.mg/l.total.dissolved.solids. • Treatment.from.frac.tanks. • Treated.at.20. bpmwww.hydrozonix.com Recycle..Reuse..Results.. 50. 55. 60. 65. 70. 75. 80.

2. 4. 6. 8. 10. 12. 14. 16.%(Fric=on(Reduc=on(

Time((min)( 

### Anionic(Fric=on(Reducer(Performance(@(0.5(Gallons(per(Thousand( Duncan.Tap.Water. Raw.Water. Treated.(One.Pass).

14. 

# Case(Study(3(–(Fric=on(Reducer(Compa=bility(


# 20.25% improvement In friction reducer performancewww.hydrozonix.com Recycle..Reuse..Results.. 

### Ozone(–(O 3( .....Ahacks.organics,.kills.bacteria. .....Oxidizes.heavy.metals. 

### Hydrodynamic(Cavita=on( .....Efficient.mass.transfer.of.ozone. .....Cavita7on.aids.in.killing.bacteria. 

### Electrochemistry( .....DC.electrodes.aid.chemical.decomposi7on. .....Creates.hydroxyl.radicals.(strong.oxidant).from.residual.O 2......... Forces.electrochemical.precipita7on.of.hardness.salts 

### Acous=c((Ultrasonic)(Cavita=on(..... Sonoluminescence .aids.in.killing.bacteria. ......Ultrasound.cleaves.precipitated.hardness.salts.into. nano >sized.suspended.par7cles.

15. 

# Ozonix TM 

# (Science(www.hydrozonix.com Recycle..Reuse..Results.. 

# www.hydrozonix.com