## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 1 of 39 Data Requirement: PMRA Data Code: 9.8.4 (TGAI) or 9.8.6 (EP) EPA DP Barcode: 427855 OECD Data Point: IIA 8.12 (TGAI) and IIIA 10.8.1.1 (EP) EPA Guideline: 850.4100

Test material: Atrazine SC Purity: 43.0% (w/w) Common name: Chemical name: IUPAC: CAS name: CAS No.: 1912-24-9 Synonyms: A8566A; G30027 SC (479.37)

Primary Reviewer: Joan Gaidos Signature: Senior Scientist, CDM Smith Date: 11/12/15

Secondary Reviewer: Teri S. Myers Signature: Senior Scientist, CDM Smith Date: 11/16/15

Secondary Reviewer: Frank T. Farruggia, Ph.D. Date: 2-29-16 {EPA/OECD/PMRA}

Reference/Submission No.: {.....................}

Company Code {............} [For PMRA] Active Code {............} [For PMRA] Use Site Category: {............} [For PMRA] EPA PC Code 080803

Date Evaluation Completed: 29-02-2016

CITATION: Martin J.A. Atrazine SC (A8566A)

– Seedling Emergence Test with Extended Exposure to View Potential Recovery. Unpublished study performed by Smithers Viscient, Wareham, Massachusetts and sponsored by Syngenta crop Protection, LLC, Greensboro, North Ca rolina. Laboratory Study Number: 1781.7000. Study completed March 13, 2015.

DISCLAIMER: This document provides guidance for EPA an d PMRA reviewers on how to complete a data evaluation record after reviewing a scientific study concerni ng the acute toxicity of a pest icide to terrestrial vascular plants. It is not intended to prescribe conditions to any external party for conducting this study nor to establish absolute criteria regarding the assessment of whether the study is scientifically sound and whether the study satisfies any applicable data requirements. Reviewer s are expected to review and to deter mine for each study, on a case-bycase basis, whether it is scientif ically sound and provides sufficient in formation to satisfy applicable data requirements. Studies that fail to meet any of the conditio ns may be accepted, if appropriate; similarly, studies that meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to tak e into account the totality of factors related to the test methodology and results in determining the acceptability of the study. Digitally signed by FRANK FARRUGGIA DN: c=US, o=U.S. Government, ou=USEPA, ou=Staff, cn=FRANK FARRUGGIA, dnQualifier=0000048865 Date: 2016.02.29 12:58:50 -05'00'

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 2 of 39 EXECUTIVE SUMMARY:

The effect of Atrazine on the seedling emergence of monocot corn, Zea mays ; onion, Allium cepa ; ryegrass, Lolium
perenne ; and oat, Avena sativa ) and dicot (cabbage, Brassica oleracea ; lettuce, Lactuca sativa ; carrot, Daucus
carota ; cucumber, Cucumis sativus ; soybean, Glycine max ; tomato, Lycopersicon esculentum ) crops was studied at a nominal concentration of 0 (negative), 0.0050, 0.0098, 0.02 5, 0.050, 0.098 and 0.25 lb ai/A for cabbage and tomato; 0 (negative), 0.0098, 0.025, 0.050, 0.098, 0.25 and 0.50 lb ai/A for soybean; 0 (negative), 0.0025, 0.0050, 0.010, 0.025, 0.050, and 0.098 lb ai/A for cucumber, onion and ryegrass; 0 (negative), 0.0010, 0.0025, 0.0050, 0.010, 0.025, and 0.050 lb ai/A for carrot, lettuce and oat; 0 (negative), 0.50, 0.98, 2.5, 5.0, 9.8 and 25 lb ai/A for corn. Measured application r ate were <LOQ (negative), 0.0046/0.0059, 0. 0099/0.0082, 0.022/0. 021, 0.049/0.047, 0.097/0.095 and 0.26/0.31 lb ai/A for cabbage and tomato; <LOQ (negative), 0.0082, 0.021, 0.047, 0.095, 0.31 and 0.44 lb ai/A for soybean; <LOQ (negative), 0.0024, 0.0047, 0.0099, 0.025, 0.047, and 0.10 lb ai/A for cucumber, onion and ryegrass; <LOQ (negative), 0.00098/0.0011, 0. 0024/0.0042, 0.0046/0.0059, 0.0099/0.0082, 0.022/0.021, and 0.049/0.047 lb ai/A for carrot, lettuce and oat; <LOQ (negative), 0.44, 0.95, 2.5, 5.0, 9.9 and 24 lb ai/A for corn. LOQ was 0.000065 lb ai/A for cabbage, carrot and lettuce; 0.00016 lb ai/A for onion, ryegrass and cucumber; and 0.000067 lb ai/A for corn, oat, soybean and tomato.

The growth medium used in the seedling emergence test wa s natural loam soil augmented with washed silica sand (pH and organic carbon not reported; 91% sand, 7% silt and 2% clay). For Test 1, on day 14 the surviving plants per pot were recorded, and plant height and dry weight were measured. For Test 2, on day 28 the surviving plants per pot were recorded, and plant height and dry weight were measured; height data was also reported for day 14 of the 28 day study.

Test 1, 14 days Based on the reviewer results, negative control emergenc e was 73-98% compared to the negative control. The reviewer found significant inhibition in emergence for cu cumber of 15% at 0.047 lb ai/A (Mann-Whitney U TwoSample test, p<0.05).

The reviewer reported negative control survival, based on number planted, ranged from 73-98% compared to the negative control. The reviewer found significant inhibitions in cabbage survival of 47% at 0.26 lb ai/A; in cucumber of 26% at 0.047 lb ai/A; and in tomato of 46% at 0.31 lb ai/A compared to the negative control (Mann-Whitney U Two-Sample test, p<0.05).

The reviewer reported dry weight inhibitions for cabbage of 62% at 0.26 lb ai/A, and for tomato of 48 and 78% at 0.095 and 0.31 lb ai/A (Williams test, p<0.05). There were inhibitions also for oat of 35% at 0.047 lb ai/A (Dunnett’s test p<0.05).

The reviewer reported height inhibitions for cabbage of 19 to 49% at 0.097 and 0.26 lb ai/A (Williams test, p<0.05); for oat of 15% at 0.047 lb ai/A (Dunnett’s test , p<0.05); and for tomato of 25 and 53% at 0.095 and 0.31 lb ai/A (Jonckheere-Terpstra Step-Down test, p<0.05).

The most sensitive monocot, based on the reviewer’s results, was oat based on dry weight with NOAEC and EC 25 

### values of 0.021 lb ai/A and 0.0456 lb ai/A, respectively; th e most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.097 and 0.0299 lb ai/A, respectively.

Based on the phytotoxicity rating system used by the study au thor, there were no effects in the negative control, and no effects for lettuce, oat, onion, and ryegrass at any app lication rate. There were effects up to 100% for cabbage, carrot, corn, cucumber, soybean and tom ato. The effects were dose-dependent.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 3 of 39 Test 2, 28 days Based on the reviewer results, negative control emergenc e was 70-98% compared to the negative control. The reviewer found no significant inhibitions in surv ival for any species at any application rate.

The reviewer reported negative control survival, based on number planted, ranged from 70-98% compared to the negative control. The reviewer found significant inhibitions in cabbage survival of 46 an d 85% at 0.097 and 0.26 lb ai/A (Dunnett’s test, p<0.05); cucumber of 22% at 0.10 lb ai/A; and in to mato of 46% at 0.31 lb ai/A compared to the negative control (Mann-Whitney U Two-Sample test, p<0.05).

The reviewer reported dry weight inhibitions for cabbage of 35, 45, 34 and 83% at 0.022, 0.049, 0.097, and 0.26 lb ai/A; for onion of 35 and 49% at 0.047 an d 0.10 lb ai/A (Williams test, p<0.05); and in tomato of 69% at 0.31 lb ai/A (Dunnett’s test, p<0.05).

The reviewer reported height inhibitions for cabbage of 12, 14, 17, 20 and 43% at 0.0099, 0.022, 0.049, 0.097 and 0.26 lb ai/A; and for onion of 15 and 18% at 0.047 and 0.10 lb ai/A (William s test, p<0.05). There were also inhibitions in height for corn of 28% at 9.9 lb ai/A, cucumber of 11 and 11% at 0.0099 and 0.047 lb ai/A; and tomato of 31% at 0.31 lb ai/A (Dunnett’s test, p<0.05).

The most sensitive monocot, based on the reviewer’s results, was onion based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.0341 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.0099 and 0.0177 lb ai/A, respectively.

Based on the phytotoxicity rating system used by the study author, there were no effects in the negative control, with the exception of an individual carrot and corn plant. Ther e were no effects for carrot, lettuce, oat, ryegrass and soybean at the application rates. There were effects up to 100% for ca bbage, corn, cucumber, onion and tomato. The effects were dose-dependent.

Test 2a, 14 days of 28 day study The reviewer was also unable to review survival at 14 days of the 28 day study due to the lack of raw data for analysis.

The reviewer reported height significant inhibitions for cabbage of 28, 34, 42 and 63% at 0.022, 0.049, 0.097 and 0.26 lb ai/A (Jonckheere-Terpstra Step-Down test, p<0.05). There were also significant inhibitions for corn of 26% at 9.9 lb ai/A; for cucumb er of 16% at 0.10 lb ai/A; and for tomato of 45% at 0.31 lb ai/A (Dunnett’s test, p<0.05).

The most sensitive monocot, based on the reviewer’s results, was onion based on height with NOAEC and EC 25 

### values of 0.10 lb ai/A and 0.159 lb ai/A, respectively; the most sensitive dicot was cabbage based on height with NOAEC and EC 25 

### values of 0.0099 and 0.0262 lb ai/A, respectively.

The phytotoxicity on day 14 of the 28 day study was not reported.

The study author also reported growth rates for height and weight, however the reviewer could not verify these rates. Study author results presented in the conclusions at the end of the DER.

Maximum Labeled Rate: 4 lb ai/A (4500 g ai/ha).

Test 1, 14 days

Most sensitive monocot: Oat, based on dry weight

EC 50 

### /IC 50 

### : 0.0497 lb ai/A 95% C.I.: 0.0488-0.0507 lb ai/A EC 25 

### /IC 25 

### : 0.0456 lb ai/A 95% C.I.: 0.0431-0.0477 lb ai/A

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 4 of 39 EC 05 

### /IC 05 

### : 0.0403 lb ai/A 95% C.I.: N/A-0.043 lb ai/A NOEC: 0.021 lb ai/A Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Cabbage, based on dry weight

EC 50 

### /IC 50 

### : 0.132 lb ai/A 95% C.I.: 0.063-0.277 lb ai/A EC 25 

### /IC 25 

### : 0.0299 lb ai/A 95% C.I.: 0.00884-0.0713 lb ai/A EC 05 

### /IC 05 

### : 0.00351 lb ai/A 95% C.I.: N/A-0.0215 lb ai/A NOEC: 0.097 lb ai/A Slope: N/A 95% C.I.: N/A

Test 2, 28 days

Most sensitive monocot: On ion, based on dry weight

EC 50 

### /IC 50 

### : 0.144 lb ai/A 95% C.I.: 0.050-0.413 lb ai/A EC 25 

### /IC 25 

### : 0.0341 lb ai/A 95% C.I.: 0.0153-0.0645 lb ai/A EC 05 

### /IC 05 

### : 0.00432 lb ai/A 95% C.I.: N/A-0.016 lb ai/A NOEC: 0.025 lb ai/A Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Cabbage, based on dry weight

EC 50 

### /IC 50 

### : 0.102 lb ai/A 95% C.I.: 0.0496-0.21 lb ai/A EC 25 

### /IC 25 

### : 0.0177 lb ai/A 95% C.I.: 0.00657-0.0389 lb ai/A EC 05 

### /IC 05 

### : 0.00143 lb ai/A 95% C.I.: N/A-0.01 lb ai/A NOEC: 0.0099 lb ai/A Slope: N/A 95% C.I.: N/A

Test 2a, 14 days of 28 days

Most sensitive monocot: Onion, based on height (only endpoint analyzed)

EC 50 

### /IC 50 

### : NC 95% C.I.: N/A EC 25 

### /IC 25 

### : 0.159 lb ai/A 95% C.I.: 0.00491-0.625 lb ai/A EC 05 

### /IC 05 

### : 0.0205 lb ai/A 95% C.I.: N/A-0.0693 lb ai/A NOEC: 0.10 lb ai/A Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Cabbage, based on height (only endpoint analyzed)

EC 50 

### /IC 50 

### : 0.132 lb ai/A 95% C.I.: 0.0872-0.199 lb ai/A EC 25 

### /IC 25 

### : 0.0262 lb ai/A 95% C.I.: 0.0158-0.0409 lb ai/A EC 05 

### /IC 05 

### : 0.00257 lb ai/A 95% C.I.: N/A-0.00834 lb ai/A NOEC: 0.0099 lb ai/A Slope: N/A 95% C.I.: N/A

This toxicity study is classified as acceptable for the herein labeled “Tes t 1-14-day Study ” . The concurrent 28-day study labeled here as Test 2 and Test 2a is classified as supplemental, as the study design did not allow for dry weight endpoints at the 14-day period, did not provide survival at 14-days.

Table 1a. Summary of most sensitive paramete rs by species (lb ai/A); Test 1, 14 days

Species Endpoint NOEC EC 05 

### /IC 05 

### EC 25 

### /IC 25 

### EC 50 

### /IC 50

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 5 of 39 Cabbage Dry weight 0.097 0.00351 0.0299 0.132 Carrot None 0.049 NC NC NC Corn* Dry weight 24 9.6 >24 NC Cucumber None 0.10 >0.10 >0.10 >0.10 Lettuce None 0.049 >0.049 >0.049 >0.049 Oat Dry weight 0.021 0.0403 0.0456 0.0497 Onion None 0.10 >0.10 >0.10 >0.10 Ryegrass None 0.10 >0.10 >0.10 >0.10 Soybean* Dry weight 0.44 0.316 >0.44 >0.44 Tomato Dry weight 0.047 0.0168 0.0527 0.117 * Not a dose response and/or ECx/ICx values are not bracketed by the test concentrations and are unreliable.

Table 1b. Summary of most sensitive paramete rs by species (lb ai/A); Test 2, 28 days

Species Endpoint NOEC EC 05 

### /IC 05 

### EC 25 

### /IC 25 

### EC 50 

### /IC 50 

### Cabbage Dry weight 0.0099 0.00143 0.0177 0.102 Carrot None 0.049 >0.049 >0.049 >0.049 Corn* Height 5.0 1.31 >24 NC Cucumber* Height 0.0047 0.00376 >0.10 NC Lettuce Dry weight 0.049 0.0222 0.0475 0.0804 Oat None 0.047 >0.047 >0.047 >0.047 Onion Dry weight 0.025 0.00432 0.0341 0.144 Ryegrass* Dry weight 0.10 0.0285 >0.10 NC Soybean* Dry weight 0.44 0.0493 >0.44 NC Tomato Dry weight 0.095 0.0776 0.146 0.225 * Not a dose response and/or ECx/ICx values are not bracketed by th e test concentrations and are unreliable.

Table 1c. Summary of most sensitive parameters by species (lb ai/A); Test 2a, 14 days of 28 days (height only endpoint analyzed)

Species Endpoint NOEC EC 05 

### /IC 05 

### EC 25 

### /IC 25 

### EC 50 

### /IC 50 

### Cabbage Height 0.0099 0.00257 0.0262 0.132 Carrot None 0.049 >0.049 >0.049 >0.049 Corn* Height 5.0 1.5 34.6 307 Cucumber* Height 0.047 0.0365 0.23 NC Lettuce* Height 0.049 0.0307 0.0645 NC Oat None 0.047 >0.047 >0.047 >0.047 Onion* Height 0.10 0.0205 0.159 NC Ryegrass None 0.10 >0.10 >0.10 >0.10 Soybean None 0.44 >0.44 >0.44 >0.44 Tomato Height 0.095 0.049 0.164 >0.31 * Not a dose response and/or ECx/ICx values are not bracketed by th e test concentrations and are unreliable.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 6 of 39 I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: This study was conducted in compliance with OPPTS 850.4100: Seedling Emergence (January 2012) and OECD Guideline No. 208: Terrestrial Plant Test: Seedling Emergence test. The reviewer evaluated the study methods according to EPA Ecological Effects Test Guidelines, OCSPP Guideline 850.4100: Seedling Emergence. There were some deficiency and deviations noted by the reviewer.

1. The physico-chemical properties of the test material were not reported.

2. Survival at 14 days in the 28 day study was not analyzed by the study author, and raw data were not provided. The reviewer coul d not perform statistical analysis or report results for this endpoint.

The deficiency and deviations did have an impact on the acceptability of this study.

COMPLIANCE: Signed and dated GLP, Quality As surance and No Data Confidentiality statements were provided. This study was conducted in compliance with USEPA (40 CFR, Part 160) and OECD (OECD, 1998) Principles of Good Laboratory Practice., with the following exception: routine water and food contaminant screening analyses for PCBs, pesticides and toxic metals were conducted using standard U.S. EPA procedures by GeoLabs, Inc, Braintree, Massachusetts and are considered facilit y records under Smithers Viscient’s Standard Operating Procedures. Sinc e the analyses were conducted following standard validated methods, these exceptions had no impact on the study results.

A. MATERIALS:

1. Test Material Atrazine SC

Description: White liquid.

Lot No./Batch No.: 726537

Purity: 43.0% (w/w)

Stability of compound under test conditions: Analytical determinations based on measured concentration of the test material in the spray solution yielded a recoveries of 85.0-117.2% of nominal; with the exception of the 0.0042 lb ai/A test concentrations for corn, oat, soybean and tomato, which was 170.0% of nominal. Stability was not determined. QC recover ies ranged from 64-119%. Method recoveries of the atrazine from 1% crop oil in deionized water were 104±7.97% (n=9).

(OECD recommends chemical stability in water and light )

Storage conditions of test chemicals: The test material was stored in the dark at room temperature.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 7 of 39 Table 2. Physical/chemical properties of Atrazine

Parameter Values Comments

Water solubility at 20  ̊ C Not reported

Vapor pressure Not reported

UV absorption Not reported

pKa Not reported

Kow Not reported

2. Test organism :

Monocotyledonous species: Corn ( Zea mays , Golden Bantam); Onion ( Allium cepa , Yellow Granex); Ryegrass ( Lolium perenne , Linn) and Oat ( Avena sativa, Swan); EPA recommends four monocots in two families, including corn. Dicotyledonous species: Lettuce ( Lactuca sativa , Prizehead); Cabbage, Brassica oleracea , Ganzales); Carrot ( Daucus carota , Imperator 58); Cucumber ( Cucumis sativus , Bush Champion); Tomato ( Lycopersicon esculentum , Celebrity Hybrid); and Soybean ( Glycine max , Envy); EPA recommends six dicots in four families, inclu ding soybean a nd a root crop.

OECD recommends a minimum of three spec ies selected for testing, at least one from each of the following categories: Category 1: ryegrass, rice, oat, wheat, and sorghum; Cate gory 2: mustard, rape, radish, turnip, and Chinese cabbage; Category 3: vetch, mung bean, red clover, fenugreek, lettuce, and cress.



### Seed source: Cabbage, onion from Park Seed Company; carrot and lettuce from Sustainable Seed Company; corn, cucumber from Burpee; oat from L. A. Hearne Compound; ryegrass from Granite Seed,

so ybean from Johnny’s Selected Seeds; and tomato from Tomato Growers Supply Company. Prior seed treatment/sterilization: The seeds were not treated with any type of fungicides, insecticides, or any pesticides. Historical % germination of seed: Carrot, 83%; lettuce, 85%; oat, 90%; ryegrass, 95%; soybean, 87%; cabbage, corn, cucumber, onion and tomato, N/A. Seed storage, if any: Seeds stored refrigerated in the dark until use.

B. STUDY DESIGN:

1. Experimental Conditions

a. Limit test: None.

b. Range-finding study:

c. Definitive Study

Table 3: Experimental Parameters

– Seedling Emergence.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 8 of 39 Parameters Seedling Emergence

Details Remarks

Criteria

Duration of the test Test 1: 14 days
Test 2: 28 days Recommended test duration is 14-21 days.

OECD recommends that th e test be terminated no sooner than 14 days after 50 percent of the control seedlings have emerged



### Number of seeds/plants/species/ replicate Oat, onion and ryegrass, 5 replicates with 8 plants per replicate; All others 10 replicates with 4 plants per replicates. Ten seeds per replicate should be used.

OECD recommends a minimum of five seeds planted in each replicate within 24 hours of incorporation of the test substance. All seeds of each species for each test should be of the same size class. The seed should not be imbibed.



### Number of replicates

Control: Adjuvant control: Treated:

Control: Adjuvant control: Treated: Oat, onion and ryegrass:
5
N/A
5
All others:
10
N/A
10 Four replicates per dose should be used.

OECD recommends a minimu m of four replicates per treatment

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 9 of 39 Parameters Seedling Emergence

Details Remarks

Criteria

Test concentrations (lb ai/A)

Nominal:

Measured: 0 (negative), 0.0050, 0.0098, 0.025, 0.050, 0.098 and 0.25 lb ai/A for cabbage and tomato; 0 (negative), 0.0098, 0.025, 0.050, 0.098, 0.25 and 0.50 lb ai/A for soybean; 0 (negative), 0.0025, 0.0050, 0.010, 0.025, 0.050, and 0.098 lb ai/A for cucumber, onion and
ryegrass; 0 (negative), 0.0010, 0.0025, 0.0050, 0.010, 0.025, and 0.050 lb ai/A for carrot, lettuce and oat; 0 (negative), 0.50, 0.98, 2.5, 5.0, 9.8 and 25 lb ai /A for corn.

<LOQ (negative), 0.0046/0.0059, 0.0099/0.0082, 0.022/0.021, 0.049/0.047, 0.097/0.095 and 0.26/0.31 lb ai/A for cabbage and tomato; <LOQ (negative), 0.0082, 0.021, 0.047, 0.095, 0.31 and 0.44 lb ai/A for soybean; <LOQ (negative), 0.0024, 0.0047, 0.0099, 0.025, 0.047, and 0.10 lb ai/A for cucumber, onion and
ryegrass; <LOQ (negative), 0.00098/0.0011, 0.0024/0.0042, 0.0046/0.0059, 0.0099/0.0082, 0.022/0.021, and 0.049/0.047 lb ai/A for carrot, lettuce and oat; <LOQ (negative), 0.44, 0.95, 2.5, 5.0, 9.9 and 24 lb ai/A for corn. LOQ = 0.000065 lb ai/A for cabbage, carrot and lettuce; LOQ = 0.00016 lb ai/A for onion, ryegrass and cucumber; LOQ = 0.000067 lb ai/A for corn, oat, soybean and tomato.

Five test concentrations should be used with a dose range of 2X or 3X progression

OECD recommends three concentrations, preferably with application rates equivalent to 0.0 (control), 1.0, 10.0 and 100 mg substance per kg of oven

- dried soil.



### Method and interval of analytical verification Samples were taken from the negative control and the treatment groups for analysis via HPLC with UV detection: Aglient Zorbax SB -C18 column and 2 24 nm wavelength

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 10 of 39 Parameters Seedling Emergence

Details Remarks

Criteria

LOQ:

LOD: 0.000065 lb ai/A for cabbage, carrot and lettuce; 0.00016 lb ai/A for onion, ryegrass and cucumber; 0.000067 lb ai/A for corn, oat, soybean and tomato.

N/A

Adjuvant (type, percentage, if used) Crop oil (AGri-Dex), 1%

Test container (pot)

Size/Volume Material: (glass/polystyrene) 13 cm tall and 26 cm top diameter (20 cm bottom diameter); 20 cm fill paper interior base. Polypropylene Non-porous containers should be used.

OECD recommends that non -porous plastic or glazed pot be used.



### Growth facility Greenhouse

Method/depth of seeding Seeds were placed in a circular pattern around the inside perimeter of the pot.

Test material application Application time including the plant growth stage

Number of application

Application interval

Method of application 1

N/A - single application

The test material was applied using a conventional agricultural overhead atomizing spray nozzle (8001-E-SS) and revolving belt.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 11 of 39 Parameters Seedling Emergence

Details Remarks

Criteria

Details of soil used Geographic location Depth of soil collection Soil texture % sand % silt % clay pH: % organic carbon CEC (meq/100 g) Moisture at 1/3 atm (%) N/A N/A Sand 91 7 2 Not reported Not reported Not reported Not reported Not reported Natural loam soil combined with washed silica sand.

Soil mixes containing sandy loam, loam, or clay loam soil with no greater than 2% organic matter are preferable. Glass beads, rock wool, and 100% acid washed sand are not preferred.

OECD prefers the soil to be sieved (0.5 cm) to remove coarse fragments. Carbon content should not exceed 1.5% (3% organic matter). Fine particles (under 20um) makeup should be between 10 and 20%. The recommended pH is between 5.0 and 7.5.



### Details of nutrient medium, if used Peters 20-20-20 (N-P-K), 400 mg/L for corn, oat, soybean and
tomato . Applied (100 mL) by sub -irrigation twice weekly.

Watering regime and schedules Water source/type: Volume applied: Interval of application: Method of application: Well water (Town of Wareham). Not reported. Daily

. The plants were bottom watered after being initially top watered (100 mL deionized water) to promote capillary action. EPA prefers that bottom watering be utilized for seedling emergence studies so that the chemical is

not leached out of the soil during the test.



### Any pest control method/fertilization, if used None reported

Test conditions Temperature: Photoperiod: Light intensity and quality:

Relative humidity: 15 -51  ̊ C
16L:8D Natural sunlight supplemented with artificial light.
69 -1500 μ mol/m 2 

### /sec
15 -98% EPA prefers that the cold vs warm loving plants be tested in two separate groups to optimize plant growth.

OECD prefers that the temperature, humidity and light conditions be suitable for maintaining normal growth of each species for the test period.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 12 of 39 Parameters Seedling Emergence

Details Remarks

Criteria

Reference chemical (if used) Name: Concentrations: N/A

Other parameters, if any None

2. Observations:

Table 4: Observation Parameters

– Seedling Emergence.

Parameters Seedling Emergence

Details Remarks

Parameters measured (e.g., number of germinated seeds, emerged seedlings, plant height, dry weight or other endpoints) - Emergence - S urvival - Height - Mean dry weight - Phytotoxicity

Measurement technique for each parameter Phytotoxicity was visually determined. Height was measured to the nearest 0.5 centimeter from the soil surface to the tallest new growth. Mean plant weight was estimated by measuring the dry weight following drying at 70 ± 5

 ̊ C

.

Observation intervals Each pot was inspected weekly and phytotoxicity assessments performed. Plant height and dry weight were recorded at study termination.

Other observations, if any N/A

Were raw data included? Yes

The health rating,

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 13 of 39 Phytotoxicity rating system, if used including morphological abnormalities ranges from 0 to 100, where 0 indicates no injury or abnormality and 100 indicates complete effect (dead plant).

II. RESULTS and DISCUSSION:

A. INHIBITORY EFFECTS:

1. Seedling Emergence:

Test 1, 14 days Based on the study author and reviewer results, ne gative control emergence was 73-98% compared to the negative control. The study author did not find any significant inhibitions in emergence for any species at any application rate. The reviewer found significant inhi bition in emergence for cucumber of 15% at 0.047 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05).

The study author reported negative control survival, based the number em erged, was 100% compared to the negative control. The study author found survival si gnificant inhibitions in cabbage of 20, 20 and 53% at 0.050, 0.098 and 0.25 lb ai/A; and in tomato of 42% at 0.25 lb ai/A (Fish er’s Exact Test with Bonferroni

Holm’s Adjustment, p<0.05). The reviewer reported negative control survival, based on number planted, ranged from 73-98% compared to the negative cont rol. The reviewer found significant inhibitions in cabbage survival of 47% at 0.26 lb ai/A; in cucumber of 26% at 0.047 lb ai/A; and in tomato of 46% at 0.31 lb ai/A compared to the negative control (Mann-Whitney U Two-Sample test, p<0.05).

The study author found significant inhibitions in dry weight compared to the negative control for cabbage of 62% at 0.25 lb ai/A; for oat of 35% at 0.050 lb ai/A ; and in tomato of 48 and 78% at 0.098 and 0.25 lb ai/A, respectively (Dunnett’s Multiple Comparison test, p<0.05) . The reviewer reported similar results, with dry weight significant inhibitions for cabbage of 62% at 0.26 lb ai/A, and fo r tomato of 48 and 78% at 0.095 and 0.31 lb ai/A, respectively (Williams test, p< 0.05). There were also significant inhibitions for oat of 35% at 0.047 lb ai/A (Dunnett’s test p<0.05).

The study author found significant inhibitions in height for cabbage of 49% at 0.25 lb ai/A, and oat of 15% at 0.050 lb ai/A compared to the negative control ( Dunnett’s Mul tiple Comparison test, p<0.05). There were inhibitions in tomato height of 25 and 5 3% at 0.098 and 0.25 lb ai/A (Wilcoxon’s Test with Bonferroni- Holm’s Adjustment, p<0.05). The reviewer reported similar results, with height significant inhibitions for cabbage of 19 and 49% at 0.097 an d 0.26 lb ai/A, respectively (Williams test, p<0.05); for oat of 15% at 0.047 lb ai/A (Dunnett’s test, p<0.05); and for tomato of 25 and 53% at 0.095 and 0.31 lb ai/A, respectively (Jonckheere-Terpstra Step-Down test, p<0.05).

The most sensitive monocot, based on the study author's results, was oat based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.039 lb ai/A, re spectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.098 and 0.010 lb ai/A, respectively.

The most sensitive monocot, based on the reviewer’s results, was oat based on dry weight with NOAEC and EC 25 

### values of 0.021 lb ai/A and 0.0456 lb ai/A, re spectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.097 and 0.0299 lb ai/A, respectively.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 14 of 39 Based on the phytotoxicity rating system used by the study author, there were no effects in the negative control, and no effects for lettuce, oat, onion, and ryegra ss at any application rate. There were effects up to 100% for cabbage, carrot, corn, cucumber, soybean and tomato. The effects were dose-dependent.

Test 2, 28 days Based on the study author and reviewer results, neg ative control emergence was 70-98% compared to the negative control. The study author and reviewer found no significant inhibitions in emergence for any species at any app lication rate.

The study author reported negative control survival, based the number emerged, was 98-100%, compared to the negative control. The study author found surviv al significant inhibitions in cabbage of 46 and 85% at 0.098 and 0.25 lb ai/A; and in tomato of 48 % at 0.25 lb ai/A (Fisher’s Exact Test with Bo nferroni- Holm’s Adjustment, p<0.05). The reviewer reported negative control survival, based on number planted, ranged from 70-98% compared to the negative control. The reviewer found significant inhibitions in cabbage survival of 46 and 85% at 0.097 and 0.26 lb ai/A, respectively (Dunnett’s test, p<0.05) ; cucumber of 22% at 0.10 lb ai/A; and in tomato of 46% at 0.31 lb ai/A compared to the negative control (Mann-Whitney U Two-Sample test, p<0.05).

The study author found significant inhibitions in dry weight for cabbage of 35, 45, 34 and 83% at 0.025, 0.050, 0.098 and 0.25 lb ai/A, respectively; for onion of 35 and 49% at 0.050 and 0.098 lb ai/A, respectively; and in tomato of 69 % at 0.25 lb ai/A (Dunnett’s Multiple Comparison test, p<0.05). The reviewer reported similar results, with dry weight sign ificant inhibitions for cabbage of 35, 45, 34 and 83% at 0.022, 0.049, 0.097, and 0.26 lb ai/A, respectivel y; onion of 35 and 49% at 0.047 and 0.10 lb ai/A, respectively (Williams test, p<0.05); and to mato of 69% at 0.31 lb ai/A (Dunnett’s test, p<0.05).

The study author found significant inhibitions in height for cabbage of 17, 20 and 43% at 0.050, 0.098 and 0.25 lb ai/A, respectively; corn of 28% at 9.8 lb ai/A; cucumber of 11 and 11% at 0.010 and 0.050 lb ai/A, respectively; and onion of 18% at 0.098 lb ai/A ( Dunnett’s Multiple Comparison test, p<0.05). There were also significant inhibitions in tomato height of 31 % at 0.25 lb ai/A (Wilcoxon’s Test with Bonferroni

Holm’s Adjustment, p<0.05). Th e reviewer reported similar results, with significant height inhibitions for cabbage of 12, 14, 17, 20 and 43% at 0.0099, 0.022, 0.049, 0.097 and 0.26 lb ai/A, respectively; and for onion of 15 and 18% at 0. 047 and 0.10 lb ai/A, respectively (Williams test, p<0.05). There were also significant inhibitions in height for corn of 28% at 9.9 lb ai/A, cucumber of 11 and 11% at 0.0099 and 0.047 lb ai/A, respectively; and tomato of 31% at 0.31 lb ai/A (Dunn ett’ s test, p<0.05).

The most sensitive monocot, based on the study author's results, was onion based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.031 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.010 and 0.014 lb ai/A, respectively.

The most sensitive monocot, based on the reviewer’s results, was onion based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.0341 lb ai/A, re spectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.0099 and 0.0177 lb ai/A, respectively.

Based on the phytotoxicity rating system used by the study author, there were no effects in the negative control, with the exception of an individual carrot and corn plant. There were no effects for carrot, lettuce, oat, ryegrass and soybean at the application rates. There were effe cts up to 100% for cabbage, corn, cucumber, onion and tomato. Th e effects were dose-dependent.

Test 2a, 14 days of 28 day study Using the study author results for the full 28 day study, survival in the negative control, based on number emerged was 98-100%. Raw data and percent inhibitions for survival at 14 days of the 28 day study was

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 15 of 39 not provided, and the study author did not report th e results of any statistical analysis (NOEC and ECx). The reviewer was also unable to review survival at 14 days of the 28 day study due to the lack of raw data for analysis.

The study author did not report the results of any statistical analysis or inhibitions in height for any species at 14 days of the 28 day study. The reviewer reported significant height inhibitions for cabbage of 28, 34, 42 and 63% at 0.022, 0.049, 0.097 and 0.26 lb ai/A, respectively (Jonckheere-Terpstra Step-Down test, p<0.05). There were also significant inhibitions for co rn of 26% at 9.9 lb ai/A; cucumber of 16% at 0.10 lb ai/A; and tomato of 45% at 0.31 lb ai/A (Dunnett’s test, p<0.05).

The most sensitive monocot and dicot could not be d etermined for the study author because the data were not analyzed.

The most sensitive monocot, based on the reviewer’s results, was onion based on height with NOAEC and EC 25 

### values of 0.10 lb ai/A and 0.159 lb ai/A, respectiv ely; the most sensitive dicot was cabbage based on height with NOAEC and EC 25 

### values of 0.0099 and 0.0262 lb ai/A, respectively.

The phytotoxicity on day 14 of the 28 day study was not reported.

B. REPORTED STATISTICS:

Emergence, survival, dry weight, and height means an d standard deviations were determined for control and treatment groups. For Test 1, the average specific growth rate was calcu lated for shoot length between test initiation and day 14 and shoot dry weight between test in itiation and day 28 (test termination). For Test 2, the average specific growth rate for shoot length was calculated from test initiation to day 14, day 14 to day 28, and test termination. The average specific growth rate for shoot dry weight was calculated using the Test 1 shoot dry weight data from day 14 and Test 2 shoot dry weight at test termination.

The No-Observed-Effect Concentration (NOEC), the highest test concentration which demonstrated no statistically adverse effect (p < 0.05) on day 14 (Test 1) and day 28 (Test 2) percent emergence, survival, shoot length, shoot dry weight and average growth rate based on shoot length and shoot dry weight when compared to the control data was determined. Fisher's Exact Test was used to establish treatment effects for percent survival data. The data were first checked for normality and homo geneity of variance using Shapiro-Wilk's and Bartlett's Test. If the data sets passed the tests for homogeneit y and normality, then the appropriate parametric procedure (i.e. Dunnett's Multiple Comparison Test) was used to deter mine the NOEC. If the data did not pass the tests for homogeneity and normality, then a non-parametric proc edure (i.e., Wilcoxon's Test with Bonferroni-Holm's Adjustment) was used to determine the NOEC. All statistical determinations were made at the 95% level of certainty, except in the case of Shapiro-Wilks' Test and Bartlett's Test, where the 99% level of certainty was applied. At termination of the tests, th e treatment data (percent survival, shoot length, shoot dry weight and average growth rate for shoot length and dry weight) were expressed as percent inhibition relative to the control. The ECx values and 95% confidence limits were determi ned by linear interpolation of response as compared with the control vs. nominal application rate. The regressions were limited to the concentration range for which a clear exposure-response relationship was observed. If less than the designated percent inhibition was observed for a biological endpoint, the EC values were empirically estimated to be greater than the highest application rate tested.

Nominal concentrations were used.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 16 of 39 Table 5: Effect of Atrazine on 14-Day Seedling Emergence (Test 1)
Species 

### Results summary for biomass (lbs ai/A)

weight (g) NOEC* IC 10 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 0.0144-0.0478 0.098 0.0066 0.0018- 0.016 0.010 0.0070- 0.107 0.143 0.024-ND N/A N/A

Carrot 0.0337-0.0414 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 0.172-0.262 25 8.9 0.4-ND >25 ND >25 ND N/A N/A

Cucumber 0.297-0.398 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Lettuce 0.0339-0.0699 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 2 0.0727-0.114 0.025 0.028 ND-0.036 0.039 0.027-ND >0.050 ND N/A N/A

Onion 0.00658-0.00978 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Ryegrass 0.0198-0.0262 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 0.322-0.541 0.50 0.072 0.062- 0.152 >0.50 ND >0.50 ND N/A N/A

Tomato 3 0.0265-0.124 0.050 0.032 0.0015- 0.052 0.054 0.027- 0.070 0.107 0.071- 0.134 N/A N/A

ND- Not determined. NC- Not calculable. *NOEC and all significant inhibitions determined by Dunnett’s Multiple Comparison Test (p<0.05), unless specified otherwise. 1 Significant decrease in cabbage weight, inhibition of 62% at the 0.25 lb ai/A. 2 Significant decrease in oat weight, inhibition of 35% at the 0.050 lb ai/A. 3 Significant decrease in tomato weight, inhibitions of 48 and 78% at 0.098 and 0.25 lb ai/A.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 17 of 39 Table 5a: Effect of Atrazine on 14-Day Seedling Emergence (Test 1) Species Results summary for height (lbs ai/A)

height (cm) NOEC* IC 10 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 2.24-4.77 0.098 0.014 0.0061- 0.037 0.107 0.021- 0.134 0.241 0.169-ND N/A N/A

Carrot 9.74-10.5 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 36.7-44.6 25 15.2 5.3-ND >25 ND >25 ND N/A N/A

Cucumber 18.5-27.6 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Lettuce 3.46-5.57 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 2 29.9-36.4 0.025 0.037 0.010-ND >0.050 ND >0.050 ND N/A N/A

Onion 11.4-16.2 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Ryegrass 22.6-24.7 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 31.5-43.1 0.50 >0.50 ND >0.50 ND >0.50 ND N/A N/A

Tomato 3 11.5-25.4 0.050 0.051 0.0049- 0.065 0.098 0.072- 0.125 0.223 0.187-ND N/A N/A

ND- Not determined. NC- Not calculable. *NOEC and all significant inhibitions determined by Dunnett’s Multiple Comparison Test (p<0.05), unless specified otherwise. 1 Significant decrease in cabbage height, inhibition of 49% at 0.25 lb ai/A. 2 Significant decrease in oat height, inhibition of 15% at 0.050 lb ai/A. 3 Significant decrease in tomato height, inhibitions of 25 and 53% at 0.098 and 0.25 lb ai/A ( Wilcoxon’s Test with Bonferroni

- Holm’s Adjustment, p<0.05).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 18 of 39 Table 5b: Effect of Atrazine on 14-Day Seedling Emergence (Test 1)
Species 

### Results summary for emergence (lbs ai/A)

% NOEC EC 10 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 85-98 0.25 >0.25 ND >0.25 ND >0.25 ND N/A N/A

Carrot 75-93 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 85-93 25 >25 ND >25 ND >25 ND N/A N/A

Cucumber 83-100 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Lettuce 70-90 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 78-98 0.050 0.031 ND >0.050 ND >0.050 ND N/A N/A

Onion 70-85 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Ryegrass 83-93 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 60-75 0.50 >0.50 ND >0.50 ND >0.50 ND N/A N/A

Tomato 80-93 0.25 >0.25 ND >0.25 ND >0.25 ND N/A N/A

ND- Not determined. NC- Not calculable.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 19 of 39 Table 5c: Effect of Atrazine on 14-Day Seedling Emergence (Test 1)
Species 

### Results summary for survival, based on # emerged (lbs ai/A)

% NOEC* EC 10 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 1 48-100 0.25 0.031 0.021- 0.098 0.116 0.043- 0.143 0.232 0.161-ND N/A N/A

Carrot 97-100 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 98-100 25 >25 ND >25 ND >25 ND N/A N/A

Cucumber 85-100 0.098 0.046 0.033-ND >0.098 ND >0.098 ND N/A N/A

Lettuce 100 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 100 0.050 0.051 ND >0.050 ND >0.050 ND N/A N/A

Onion 97-100 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Ryegrass 100 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 95-100 0.50 >0.50 ND >0.50 ND >0.50 ND N/A N/A

Tomato 2 58-100 0.098 0.116 0.098- 0.134 0.161 0.134- 0.232 0.25 ND N/A N/A

ND- Not determined. NC- Not calculable. *NOEC and all significant inhibitions determined by Fisher’s Exact Test with Bonferroni

- Holm’s Adjustment (p<0.05). 1 Significant decrease in cabbage survival, inhibition of 20, 20 and 53% at 0.050, 0.098 and 0.25 lb ai/A. 2 Significant decrease in tomato survival, inhibition of 42% at 0.25 lb ai/A.



### Plant Injury Index*; Test 1

Control Cabbage Carrot Corn Cucumber Lettuce Oat Onion Ryegrass Soybean Tomato Formulation Blank

0 0-100 0-100 0-100 0-100 0 0 0 0 0-100 0-100 N/A

* The health rating, including morphological abnormalities ranges fr om 0 to 100, where 0 indicates no injury or abnormality and 1 00 indicates complete effect (dead plant).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 20 of 39 Table 6: Effect of Atrazine on 28-Day Seedling Emergence (Test 2)
Species 

### Results summary for biomass (lbs ai/A)

weight (g) NOEC* IC 10 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 0.161-0.925 0.010 0.0054 0.0010- 0.01 4 0.014 0.0046- 0.098 0.125 0.024- 0.152 N/A N/A

Carrot 0.454-0.633 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 0.799-1.6 25 5.8 0.11-6.7 7.7 2.3-18.7 >25 ND N/A N/A

Cucumber 1.8-2.29 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Lettuce 0.755-1.14 0.050 0.029 0.013- 0.037 >0.050 ND >0.050 ND N/A N/A

Oat 0.458-0.592 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Onion 2 0.0285-0.0557 0.025 0.0015 ND-0.045 0.031 ND-0.080 >0.098 ND N/A N/A

Ryegrass 0.187-0.225 0.098 0.037 ND-0.107 >0.098 ND >0.098 ND N/A N/A

Soybean 0.938-1.43 0.50 0.107 0.129-ND >0.50 ND >0.50 ND N/A N/A

Tomato 3 0.426-1.46 0.098 0.088 0.0024- 0. 116 0.125 0.073- 0. 143 0.187 0.152- 0. 223 N/A N/A

ND- Not determined. NC- Not calculable. *NOEC and all significant inhibitions determined by Dunnett’s Multiple Comparison Test (p<0.05), unless specified otherwise. 1 Significant decrease in cabbage weight, inhibition of 35, 45, 34 and 83% at 0.025, 0.050, 0.098 and 0.25 lb ai/A. 2 Significant decrease in onion weight, inhibition of 35 and 49% at the 0.050 and 0.098 lb ai/A. 3 Significant decrease in tomato weight, inhibitions of 69% at 0.25 lb ai/A.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 21 of 39 Table 6a: Effect of Atrazine on 28-Day Seedling Emergence (Test 2) Species Results summary for height (lbs ai/A)

height (cm) NOEC* IC 10 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 8.93-15.8 0.025 0.0087 0.0065- 0.030 0.12 0.062- ND >0.25 ND N/A N/A

Carrot 23.4-26.4 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 2 59.7-82.8 25 6.6 0.13-8.3 >25 ND >25 ND N/A N/A

Cucumber 3 36.4-41.3 0.0050 0.010 0.0062- ND >0.098 ND >0.098 ND N/A N/A

Lettuce 13.7-15.1 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 57.6-64.6 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Onion 4 19.5-23.8 0.050 >0.026 ND-0.098 >0.098 ND >0.098 ND N/A N/A

Ryegrass 36.9-40.5 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 81.3-111 0.50 >0.48 0.017-ND >0.50 ND >0.50 ND N/A N/A

Tomato 5 43-62.5 0.098 0.116 0.0223- 0.196 0.205 0.143-ND >0.25 ND N/A N/A

ND- Not determined. NC- Not calculable. *NOEC and all significant inhibitions determined by Dunnett’s Multiple Comparison Test (p<0.05), unless specified otherwise. 1 Significant decrease in cabbage height, inhibition of 17, 20 and 43% at 0.050, 0.098 and 0.25 lb ai/A. 2 Significant decrease in corn height, inhibition of 28% at 9.8 lb ai/A. 3 Significant decrease in cucumber height, inhibition of 11 and 11% at 0.010 and 0.050 lb ai/A; inhibitions of 9 and 8% at 0.02 5 and 0.098 lb ai/A were not significant and attributed to atypically high CVs. NOEC was empirically estimated to be 0.0050 lb ai/A due to a c lear dose
response. 4 Significant decrease in onion height, inhibitions of 18% at 0.098 lb ai/A. 5 Significant decrease in tomato height, inhibition of 31% at 0.25 lb ai/A (Wilcoxon’s Test with Bonferroni

- Holm’s Adjustment).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 22 of 39 Table 6b: Effect of Atrazine on 28-Day Seedling Emergence (Test 2)
Species 

### Results summary for emergence (lbs ai/A)

% NOEC EC 10 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 88-98 0.25 >0.25 ND >0.25 ND >0.25 ND N/A N/A

Carrot 75-93 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 75-88 25 12.5 0.68-ND >25 ND >25 ND N/A N/A

Cucumber 83-100 0.098 0.080 0.060-ND >0.098 ND >0.098 ND N/A N/A

Lettuce 83-90 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 80-98 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Onion 70-88 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Ryegrass 85-95 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 68-95 0.50 >0.50 ND >0.50 ND >0.50 ND N/A N/A

Tomato 83-98 0.25 >0.25 ND >0.25 ND >0.25 ND N/A N/A

ND- Not determined. NC- Not calculable.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 23 of 39 Table 6c: Effect of Atrazine on 28-Day Seedling Emergence (Test 2)
Species 

### Results summary for survival, based on # emerged (lbs ai/A)

% NOEC* EC 10 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 1 15-100 0.050 0.051 0.014- 0.054 0.067 0.059- 0.079 0.107 0.087- 0.143 N/A N/A

Carrot 94-100 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Corn 95-100 25 >25 ND >25 ND >25 ND N/A N/A

Cucumber 86-100 0.098 0.078 0.064-ND >0.098 ND >0.098 ND N/A N/A

Lettuce 100 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Oat 95-100 0.050 >0.050 ND >0.050 ND >0.050 ND N/A N/A

Onion 83-100 0.098 0.064 ND-0.035 >0.098 ND >0.098 ND N/A N/A

Ryegrass 100 0.098 >0.098 ND >0.098 ND >0.098 ND N/A N/A

Soybean 100 0.50 >0.50 ND >0.50 ND >0.50 ND N/A N/A

Tomato 2 52-100 0.098 0.116 0.107- 0.125 0.161 0.134- 0.214 >0.25 ND N/A N/A

ND- Not determined. NC- Not calculable. *NOEC and all significant inhibitions determined by Fisher’s Exact Test with Bonferroni

- Holm’s Adjustment (p<0.05). 1 Significant decrease in cabbage survival, inhibition of 46 and 85% at 0.098 and 0.25 lb ai/A. 2 Significant decrease in tomato survival, inhibition of 48% at 0.25 lb ai/A.



### Plant Injury Index*; Test 2

Control Cabbage Carrot Corn Cucumber Lettuce Oat Onion Ryegrass Soybean Tomato Formulation Blank

0-100 0-100 0 0-100 0-100 0 0 0-100 0 0 0-100 N/A

* The health rating, including morphological abnormalities ranges fr om 0 to 100, where 0 indicates no injury or abnormality and 1 00 indicates complete effect (dead plant).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 24 of 39 Table 7: Effect of Atrazine on 14 Days of 28-Day Seedling Emergenc e (Test 2a); Not Reported Species Results summary for height (lbs ai/A)

height (cm) NOEC IC 10 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 2-5.43 NR N/A N/A N/A N/A N/A N/A N/A N/A

Carrot 9.91-22 NR N/A N/A N/A N/A N/A N/A N/A N/A

Corn 32-43.8 NR N/A N/A N/A N/A N/A N/A N/A N/A

Cucumber 17.5-20.9 NR N/A N/A N/A N/A N/A N/A N/A N/A

Lettuce 4.8-6.31 NR N/A N/A N/A N/A N/A N/A N/A N/A

Oat 30.3-37.2 NR N/A N/A N/A N/A N/A N/A N/A N/A

Onion 11.7-14.9 NR N/A N/A N/A N/A N/A N/A N/A N/A

Ryegrass 21.9-25.2 NR N/A N/A N/A N/A N/A N/A N/A N/A

Soybean 29.9-38.6 NR N/A N/A N/A N/A N/A N/A N/A N/A

Tomato 12.7-23.5 NR N/A N/A N/A N/A N/A N/A N/A N/A

ND- Not determined. NC- Not calculable. NR- Not reported

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 25 of 39 C. VERIFICATION OF STATISTICAL RESULTS BY THE REVIEWER:

All analyses were conducted comparing treated to the ne gative control. These analyses were conducted using CETIS version 1.8.7.12 and backend settings approved fo r use by EFED on 9/30/15. Data for each endpoint were tested to determine if their distributions were normal and if their variances were homogeneous using Shapiro- Wilk’s and Levene’s tests, respectively. Data that satisfied these assumptions were subjected to Dunnett’s and William’s tests, and data that did not satisfy these assumpti ons were subjected to the nonparametric Mann Whitney- U and Jonckheere’s tests. Measured concentrations were used for all analyses

. Linear (survival and emergence) and nonlinear (height and dry weight) regression models were used to interpret EC/ICx values.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 26 of 39 Table 8: Effect of Atrazine on 14-Day Seedling Emergence (Test 1)
Species 

### Results summary for biomass (lbs ai/A)

weight (g) NOEC IC 05 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 0.0144-0.0478 0.097 0.00351 N/A0.0215 0.0299 0.00884- 0.07 13 0.132 0.063- 0.277 N/A N/A

Carrot 0.0337-0.0414 0.049 0.0154 N/A-0.108 NC N/A NC N/A N/A N/A

Corn 0.172-0.262 24 9.6 N/A-23.8 24.8 9.85-42.1 NC N/A N/A N/A

Cucumber 0.297-0.398 0.10 >0.10 N/A >0.10 N/A >0.10 N/A N/A N/A

Lettuce 0.0339-0.0699 0.049 >0.049 N/A >0.049 N/A >0.049 N/A N/A N/A

Oat 2 0.0727-0.114 0.021 0.0403 N/A-0.043 0.0456 0.0431- 0.0477 0.0497 0.0488- 0.0507 N/A N/A

Onion 0.00658-0.00978 0.10 >0.10 N/A >0.10 N/A >0.10 N/A N/A N/A

Ryegrass 0.0198-0.0262 0.10 0.143 N/A-44600 >0.10 N/A >0.10 N/A N/A N/A

Soybean 0.322-0.541 0.44 0.316 0.154- 0.522 0.68 0.138- 2.31 NC N/A N/A N/A

Tomato 3 0.0265-0.124 0.047 0.0168 N/A0.0317 0.0527 0.0323- 0.0769 0.117 0.0884- 0.154 N/A N/A

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage weight, inhibition of 62% at the 0.26 lb ai/A (Williams test, p<0.05). 2 Significant decrease in oat weight, inhibition of 35% at the 0.047 lb ai/A (Dunnett’s test, p<0.05). 3 Significant decrease in tomato weight, inhibitions of 48 and 78% at 0.095 and 0.31 lb ai/A (Williams test, p<0.05).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 27 of 39 Table 8a: Effect of Atrazine on 14-Day Seedling Emergence (Test 1) Species Results summary for height (lbs ai/A)

height (cm) NOEC IC 05 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 2.24-4.77 0.049 0.0137 N/A0.0317 0.0827 0.0504- 0.12 6 0.288 0.165- 0.503 N/A N/A

Carrot 9.74-10.5 0.049 0.0469 N/A-0.112 NC N/A NC N/A N/A N/A

Corn 36.7-44.6 24 12.5 N/A-25.3 37.6 N/A-95 NC N/A N/A N/A

Cucumber 18.5-27.6 0.10 NC N/A NC N/A >0.10 N/A N/A N/A

Lettuce 3.46-5.57 0.049 >0.049 N/A >0.049 N/A >0.049 N/A N/A N/A

Oat 2 29.9-36.4 0.021 0.0347 N/A0.0645 0.0554 N/A-0.113 NC N/A N/A N/A

Onion 11.4-16.2 0.10 >0.10 N/A >0.10 N/A >0.10 N/A N/A N/A

Ryegrass 22.6-24.7 0.10 >0.10 N/A >0.10 N/A >0.10 N/A N/A N/A

Soybean 31.5-43.1 0.44 0.17 N/A-0.739 0.778 N/A-10.1 NC N/A N/A N/A

Tomato 3 11.5-25.4 0.047 0.0309 0.00724- 0.049 0.113 0.0845- 0.146 0.279 0.221- 0.351 N/A N/A

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage height, inhibition of 19 and 49% at 0.097 and 0.26 lb ai/A (Williams test, p<0.05). 2 Significant decrease in oat height, inhibition of 15% at 0.047 lb ai/A (Dunnett’s test, p<0.05). 3 Significant decrease in tomato height, inhibitions of 25 and 53% at 0.095 and 0.31 lb ai/A (Jonckheere-Terpstra Step-Down tes t, p<0.05).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 28 of 39 Table 8b: Effect of Atrazine on 14-Day Seedling Emergence (Test 1) Species Results summary for emergence (lbs ai/A)

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 85-98 0.26 NC N/A NC N/A NC N/A N/A N/A

Carrot 1 75-93 0.049 0.148 1.06E+13- 0.0246 0.000524 0.00219- N/A 0.0000104 0.000229- N/A N/A N/A

Corn 85-93 24 NC N/A NC N/A NC N/A N/A N/A

Cucumber 2 83-100 0.025 NC N/A NC N/A NC N/A N/A N/A

Lettuce 70-90 0.049 NC N/A NC N/A NC N/A N/A N/A

Oat 78-98 0.047 0.0026 9.85E-06- 0.0068 0.116 0.0338- 620 1.62 0.166- 1.02E+08 0.588 0.128-1.05

Onion 70-85 0.10 NC N/A NC N/A NC N/A N/A N/A

Ryegrass 83-93 0.10 NC N/A NC N/A NC N/A N/A N/A

Soybean 60-75 0.44 NC N/A NC N/A NC N/A N/A N/A

Tomato 80-93 0.31 NC N/A NC N/A NC N/A N/A N/A

ND- Not determined. NC- Not calculable. 1 Carrot dose-response represents an inverse relationship 2 Significant decrease in cucumber emergence, inhibition of 15% at 0.047 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05); CV%= 25%.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 29 of 39 Table 8c: Effect of Atrazine on 14-Day Seedling Emergence (Test 1)
Species 

### Results summary for survival (lbs ai/A); number survived of planted

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 1 45-98 0.097 0.00775 0.00171- 0.0159 0.0641 0.0379- 0.114 0.278 0.148-0.99 1.06 0.626-1.49

Carrot 2 75-93 0.049 0.127 75100- 0.0251 0.000808 0.00263- 2.44E-07 0.000024 0.000302- N/A N/A N/A

Corn 85-93 24 NC N/A NC N/A NC N/A N/A N/A

Cucumber 3 73-100 0.025 NC N/A NC N/A NC N/A N/A N/A

Lettuce 65-90 0.049 NC N/A NC N/A NC N/A N/A N/A

Oat 78-98 0.047 0.00206 6.17E-07- 0.00595 0.125 0.0335- 15100 2.17 0.179- 1E+10 0.545 0.0955- 0.994

Onion 70-85 0.10 NC N/A NC N/A NC N/A N/A N/A

Ryegrass 83-93 0.10 NC N/A NC N/A NC N/A N/A N/A

Soybean 60-75 0.44 NC N/A NC NA N/C N/A N/A N/A

Tomato 4 48-93 0.095 0.0034 0.00013- 0.0103 0.0761 0.0362- 0.229 0.661 0.223-16.1 0.719 0.333-1.1

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage survival, inhibition of 47% at 0.26 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05). 2 Carrot dose-response represents an inverse relationship. 3 Significant decrease in cucumber survival, inhibition of 26% at 0.047 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05); CV = 50%. 4 Significant decrease in tomato survival, inhibition of 46% at 0.31 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05).



### Plant Injury Index*; Test 1

Control Cabbage Carrot Corn Cucumber Lettuce Oat Onion Ryegrass Soybean Tomato Formulation Blank

0 0-100 0-100 0-100 0-100 0 0 0 0 0-100 0-100 N/A

* The health rating, including morphological abnormalities ranges fr om 0 to 100, where 0 indicates no injury or abnormality and 1 00 indicates complete effect (dead plant).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 30 of 39 Table 9: Effect of Atrazine on 28-Day Seedling Emergence (Test 2)
Species 

### Results summary for biomass (lbs ai/A)

weight (g) NOEC IC 05 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 0.161-0.925 0.0099 0.00143 N/A-0.01 0.0177 0.00657- 0.0389 0.102 0.0496- 0.21 N/A N/A

Carrot 0.454-0.633 0.049 >0.049 N/A >0.049 N/A >0.049 N/A N/A N/A

Corn 0.799-1.6 24 NC N/A NC N/A NC N/A N/A N/A

Cucumber 1.8-2.29 0.10 0.0192 N/A-0.252 NC N/A NC N/A N/A N/A

Lettuce 0.755-1.14 0.049 0.0222 N/A0.0409 0.0475 0.0333- 0.0 621 0.0804 0.0229- 0.282 N/A N/A

Oat 0.458-0.592 0.047 >0.047 N/A >0.047 N/A >0.047 N/A N/A N/A

Onion 2 0.0285-0.0557 0.025 0.00432 N/A-0.016 0.0341 0.0153- 0.0645 0.144 0.05- 0.413 N/A N/A

Ryegrass 0.187-0.225 0.10 0.0285 N/A0.0887 0.161 N/A-0.65 NC N/A N/A N/A

Soybean 0.938-1.43 0.44 0.0493 N/A-0.326 0.639 0.00688- 3.58 NC N/A N/A N/A

Tomato 3 0.426-1.46 0.095 0.0776 N/A-0.118 0.146 0.081- 0.205 0.225 0.181- 0.28 N/A N/A

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage weight, inhibition of 35, 45, 34 and 83% at 0.022, 0.049, 0.097 and 0.26 lb ai/A (Williams te st, p<0.05). 2 Significant decrease in onion weight, inhibition of 35 and 49% at the 0.047 and 0.10 lb ai/A (Williams test, p<0.05). 3 Significant decrease in tomato weight, inhibitions of 69% at 0.31 lb ai/A ( Dunnett’s test, p<0.05).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 31 of 39 Table 9a: Effect of Atrazine on 28-Day Seedling Emergence (Test 2) Species Results summary for height (lbs ai/A)

height (cm) NOEC IC 05 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 8.93-15.8 0.0046 0.0063 4.87E-05- 0.01 78 0.103 0.059-0.17 0.724 0.174-3.01 N/A N/A

Carrot 23.4-26.4 0.049 >0.049 N/A >0.049 N/A >0.049 N/A N/A N/A

Corn 2 59.7-82.8 5.0 1.31 N/A-7.24 38 3.03-196 NC N/A N/A N/A

Cucumber 3 36.4-41.3 0.0047 0.00376 7.69E-05- 0.0264 3.17 2.99E-05- 290 NC N/A N/A N/A

Lettuce 13.7-15.1 0.049 >0.049 N/A >0.049 N/A >0.049 N/A N/A N/A

Oat 57.6-64.6 0.047 >0.047 N/A >0.047 N/A >0.047 N/A N/A N/A

Onion 4 19.5-23.8 0.025 0.00311 N/A0.0204 0.384 0.0116- 3.81 10.9 2.29E-05- 5200000 N/A N/A

Ryegrass 36.9-40.5 0.10 NC N/A NC N/A NC N/A N/A N/A

Soybean 81.3-111 0.44 0.0522 N/A-0.341 1.4 N/A-25.8 NC N/A N/A N/A

Tomato 5 43-62.5 0.095 0.246 N/A-0.267 0.302 0.283- 0.319 0.349 0.34-0.358 N/A N/A

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage height, inhibition of 12, 14, 17, 20 and 43% at 0.0099, 0.022, 0.049, 0.097 and 0.26 lb ai/A (Williams test, p<0.05). 2 Significant decrease in corn height, inhibition of 28% at 9.9 lb ai/A (Dunnett’s test, p<0.05). 3 Significant decrease in cucumber height, inhibition of 11 and 11% at 0.0099 and 0.047 lb ai/A (Dunnett’s test, p<0.05). 4 Significant decrease in onion height, inhibitions of 15 and 18% at 0.047 and 0.10 lb ai/A (Williams test, p<0.05). 5 Significant decrease in tomato height, inhibition of 31% at 0.31 lb ai/A (Dunnett’s test, p<0.05).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 32 of 39 Table 9b: Effect of Atrazine on 28-Day Seedling Emergence (Test 2)
Species 

### Results summary for emergence (lbs ai/A)

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 88-98 0.26 NC N/A NC N/A NC N/A N/A N/A

Carrot 75-93 0.049 NC N/A NC N/A NC N/A N/A N/A

Corn 75-88 24 NC N/A NC N/A NC N/A N/A N/A

Cucumber 83-100 0.10 NC N/A NC N/A NC N/A N/A N/A

Lettuce 83-90 0.049 NC N/A NC N/A NC N/A N/A N/A

Oat 80-98 0.047 NC N/A NC N/A NC N/A N/A N/A

Onion 70-88 0.10 NC N/A NC N/A NC N/A N/A N/A

Ryegrass 85-95 0.10 NC N/A NC N/A NC N/A N/A N/A

Soybean 68-95 0.44 >0.44 N/A >0.44 N/A >0.44 N/A N/A N/A

Tomato 83-98 0.31 NC N/A NC N/A NC N/A N/A N/A

ND- Not determined. NC- Not calculable.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 33 of 39 Table 9c: Effect of Atrazine on 28-Day Seedling Emergence (Test 2)
Species 

### Results summary for survival (lbs ai/A); number survived of planted

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Cabbage 1 15-98 0.049 0.00466 0.00177- 0.00838 0.0284 0.0186- 0.0401 0.1 0.07-0.161 1.24 0.894-1.58

Carrot 73-93 0.049 NC N/A NC N/A NC N/A N/A N/A

Corn 73-85 24 NC N/A NC N/A NC N/A N/A N/A

Cucumber 2 73-100 0.047 0.0152 0.00328- 0.0297 0.193 0.0805- 3.73 1.12 0.265-302 0.88 0.346-1.41

Lettuce 83-90 0.049 NC N/A NC N/A NC N/A N/A N/A

Oat 80-98 0.047 NC N/A NC N/A NC N/A N/A N/A

Onion 70-80 0.10 NC N/A NC N/A NC N/A N/A N/A

Ryegrass 85-95 0.10 NC N/A NC N/A NC N/A N/A N/A

Soybean 68-95 0.44 >0.44 N/A >0.44 N/A >0.44 N/A N/A N/A

Tomato 3 45-93 0.095 0.00916 0.00152- 0.0201 0.103 0.0565- 0.255 0.557 0.233-4.46 0.922 0.497-1.35

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage survival, inhibition of 46 and 85% at 0.097 and 0.26 lb ai/A ( Dunnett’s test, p<0.05). 2 Significant decrease in cucumber survival, inhibition of 22% at 0.10 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05); CV = 4 1%. 3 Significant decrease in tomato survival, inhibition of 46% at 0.31 lb ai/A (Mann-Whitney U Two-Sample test, p<0.05).



### Plant Injury Index*; Test 2

Control Cabbage Carrot Corn Cucumber Lettuce Oat Onion Ryegrass Soybean Tomato Formulation Blank

0-100 0-100 0 0-100 0-100 0 0 0-100 0 0 0-100 N/A

* The health rating, including morphological abnormalities ranges fr om 0 to 100, where 0 indicates no injury or abnormality and 1 00 indicates complete effect (dead plant).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 34 of 39 Table 10: Effect of Atrazine on 14 days of 28-Day Seedlin g Emergence (Test 2a) Species Results summary for height (lbs ai/A)

height (cm) NOEC IC 05 95%CI IC 25 95%CI IC 50 95%CI slope 95%CI

Cabbage 1 2-5.43 0.0099 0.00257 N/A0.00834 0.0262 0.0158- 0.0409 0.132 0.0872- 0.199 N/A N/A

Carrot 9.91-22 0.049 >0.049 N/A >0.049 N/A >0.049 N/A N/A N/A

Corn 2 32-43.8 5.0 1.5 N/A-6.58 34.6 5.22-131 307 0.311- 303000 N/A N/A

Cucumber 3 17.5-20.9 0.047 0.0365 N/A0.0759 0.23 N/A-0.848 NC N/A N/A N/A

Lettuce 4.8-6.31 0.049 0.0307 N/A0.0627 0.0645 N/A-0.152 NC N/A N/A N/A

Oat 30.3-37.2 0.047 >0.047 N/A >0.047 N/A >0.047 N/A N/A N/A

Onion 11.7-14.9 0.10 0.0205 N/A0.0693 0.159 0.00491- 0.625 NC N/A N/A N/A

Ryegrass 21.9-25.2 0.10 >0.10 N/A >0.10 N/A >0.10 N/A N/A N/A

Soybean 29.9-38.6 0.44 >0.44 N/A >0.44 N/A >0.44 N/A N/A N/A

Tomato 4 12.7-23.5 0.095 0.049 N/A0.0808 0.164 0.119- 0.214 0.378 0.275-0.52 N/A N/A

ND- Not determined. NC- Not calculable. 1 Significant decrease in cabbage height, inhibition of 28, 34, 42 and 63% at 0.022, 0.049, 0.097 and 0.26 lb ai/A (Jonckheere- Terpstra StepDown test, p<0.05). 2 Significant decrease in corn height, inhibition of 26% at 9.9 lb ai/A ( Dunnett’s test, p<0.05). 3 Significant decrease in cucumber height, inhibition of 16% at 0.10 lb ai/A ( Dunnett’s test, p<0.05). 4 Significant decrease in tomato height, inhibition of 45% at 0.31 lb ai/A (Dunnett ’ s test, p<0.05).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 35 of 39 Test 1, 14 days

Most sensitive monocot: Oat, based on dry weight

EC 50 

### /IC 50 

### : 0.0497 lb ai/A 95% C.I.: 0.0488-0.0507 lb ai/A EC 25 

### /IC 25 

### : 0.0456 lb ai/A 95% C.I.: 0.0431-0.0477 lb ai/A EC 05 

### /IC 05 

### : 0.0403 lb ai/A 95% C.I.: N/A-0.043 lb ai/A NOEC: 0.021 lb ai/A Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Cabbage, based on dry weight

EC 50 

### /IC 50 

### : 0.132 lb ai/A 95% C.I.: 0.063-0.277 lb ai/A EC 25 

### /IC 25 

### : 0.0299 lb ai/A 95% C.I.: 0.00884-0.0713 lb ai/A EC 05 

### /IC 05 

### : 0.00351 lb ai/A 95% C.I.: N/A-0.0215 lb ai/A NOEC: 0.097 lb ai/A Slope: N/A 95% C.I.: N/A

Test 2, 28 days

Most sensitive monocot: Onion, based on dry weight

EC 50 

### /IC 50 

### : 0.144 lb ai/A 95% C.I.: 0.050-0.413 lb ai/A EC 25 

### /IC 25 

### : 0.0341 lb ai/A 95% C.I.: 0.0153-0.0645 lb ai/A EC 05 

### /IC 05 

### : 0.00432 lb ai/A 95% C.I.: N/A-0.016 lb ai/A NOEC: 0.025 lb ai/A Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Cabbage, based on dry weight

EC 50 

### /IC 50 

### : 0.102 lb ai/A 95% C.I.: 0.0496-0.21 lb ai/A EC 25 

### /IC 25 

### : 0.0177 lb ai/A 95% C.I.: 0.00657-0.0389 lb ai/A EC 05 

### /IC 05 

### : 0.00143 lb ai/A 95% C.I.: N/A-0.01 lb ai/A NOEC: 0.0099 lb ai/A Slope: N/A 95% C.I.: N/A

Test 2a, 14 days of 28 days

Most sensitive monocot: Onion, based on height (only endpoint analyzed)

EC 50 

### /IC 50 

### : NC 95% C.I.: N/A EC 25 

### /IC 25 

### : 0.159 lb ai/A 95% C.I.: 0.00491-0.625 lb ai/A EC 05 

### /IC 05 

### : 0.0205 lb ai/A 95% C.I.: N/A-0.0693 lb ai/A NOEC: 0.10 lb ai/A Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Cabbage, based on height (only endpoint analyzed)

EC 50 

### /IC 50 

### : 0.132 lb ai/A 95% C.I.: 0.0872-0.199 lb ai/A EC 25 

### /IC 25 

### : 0.0262 lb ai/A 95% C.I.: 0.0158-0.0409 lb ai/A EC 05 

### /IC 05 

### : 0.00257 lb ai/A 95% C.I.: N/A-0.00834 lb ai/A NOEC: 0.0099 lb ai/A Slope: N/A 95% C.I.: N/A

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 36 of 39 D. STUDY DEFICIENCIES:

1. The physico-chemical properties of the test material were not reported.

2. Survival at 14 days in the 28 day study was not analyzed by the study author, and raw data were not provided. The reviewer could not perform statis tical analysis or report results for this endpoint.

E. REVIEWER ’ S COMMENTS: The reviewer and study author results are in general agreement. There were some differences in NOEC and ECx values. These slight disc repancies are likely due to the reviewer using measured concentrations and differences in statistical analysis methods.

Test 1, 14 days The most sensitive monocot, based on the study author's results, was oat based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.039 lb ai/A, respectivel y; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.098 and 0.010 lb ai/A, respectively. The most sensitive monocot, based on the reviewer’s results, was oat based on dry weight with NOAEC and EC 25 

### values of 0.021 lb ai/A and 0.0456 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.097 and 0.0299 lb ai/A, respectively.

Test 2, 28 days The most sensitive monocot, based on the study author's results, was onion based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.031 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.010 and 0.014 lb ai/A, respectively. The most sensitive monocot, based on the reviewer’s results, was onion based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.0341 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.0099 and 0.0177 lb ai/A, respectively.

Test 2a, 14 days of 28 days The most sensitive monocot and dicot could not be deter mined for the study author because the data were not analyzed. The most sensitive monocot, based on the reviewer’s results, was onion based on height with NOAEC and EC 25 

### values of 0.10 lb ai/A and 0.159 lb ai/A, respec tively; the most sensitive dicot was cabbage based on height with NOAEC and EC 25 

### values of 0.0099 and 0.0262 lb ai/A, respectively.

The in-life portion of this study was initiated on April 4, 2014 to September 14, 2014.

F. CONCLUSIONS:

This toxicity study is classified as acceptable for the herein labeled “Tes t 1-14-day Study ” . The concurrent 28- day study labeled here as Test 2 and Test 2a is classif ied as supplemental, as the study design did not allow for dry weight endpoints at the 14-day period, did not provide survival at 14-days.

Test 1, 14 days The most sensitive monocot, based on the reviewer’s results, was oat based on dry weight with NOAEC and EC 25 

### values of 0.021 lb ai/A and 0.0456 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.097 and 0.0299 lb ai/A, respectively.

Most sensitive monocot and EC 25 

### /IC 25 

### : Oat (dry weight, 0.0456 lb ai/A).

Most sensitive dicot and EC 25 

### /IC 25 

### : Cabbage (dry weight, 0.0299 lb ai/A).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 37 of 39 Test 2, 28 days The most sensitive monocot, based on the reviewer’s results, was onion based on dry weight with NOAEC and EC 25 

### values of 0.025 lb ai/A and 0.0341 lb ai/A, respectively; the most sensitive dicot was cabbage based on dry weight with NOAEC and EC 25 

### values of 0.0099 and 0.0177 lb ai/A, respectively.

Most sensitive monocot and EC 25 

### /IC 25 

### : Onion (dry weight, 0.0341 lb ai/A).

Most sensitive dicot and EC 25 

### /IC 25 

### : Cabbage (dry weight, 0.0177 lb ai/A).

Test 2a, 14 days of 28 days The most sensitive monocot, based on the reviewer’s results, was onion based on height with NOAEC and EC 25 

### values of 0.10 lb ai/A and 0.159 lb ai/A, respectively; the most sensitive dicot was cabbage based on height with NOAEC and EC 25 

### values of 0.0099 and 0.0262 lb ai/A, respectively.

Most sensitive monocot and EC 25 

### /IC 25 

### : Onion (height, 0.159 lb ai/A).

Most sensitive dicot and EC 25 

### /IC 25 

### : Cabbage (height, 0.0262 lb ai/A).

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 38 of 39 Study author Growth Rate Results: The study author analyzed and reported growth rate endpoint results for shoot length and dry weight. Results are for Test 1, 14 day study and Test 2, 14-28 days. For the Test 2, 14-28 days, the study author determined dry weight growth rate using the dry weights at the end of the 14 day st udy relative to the dry weights at the end of the 28 day study. All results using nominal concentration are in g ai/ha.

## Data Evaluation Record on the Acute Toxicity of Atrazine to Terrestrial Vascular Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49639102

Page 39 of 39 III. REFERENCES:



## 1. Ives, Michael, 2013. CETIS, Comprehensive Environmental Toxicity Information SystemTM. User's Guide. Tidepool Scientific Software, McKinleyville, California.



## 2. Chetram, R.S., 1989. Tier 2 Seedling Emergence Nontarget Phytotoxicity Test. Pan-Agricultural Laboratory, Inc. Madera, California, Final report date, August 23,1989. 346 pp.



## 3. OECD, 1998. OECD Series on Principles of Good Laboratory Practice and Compliance Monitoring. Number 1. OECD Principles on Go od Laboratory Practice (as revised in 1997). Environment Directorate Chemicals Group and Management Committee. ENV/MC/CHEM(98)17. OECD Paris, France. 41pp.



## 4. OECD, 2006. OECD Guidelines for the Testing of Ch emicals. Terrestrial Plant Test 208: Seedling Emergence and Seedling Growth Test.



## 5. U.S. EPA, 1989. Federal Insecticide, Fungicide and Rodenticide Act (FIFRA); Good Laboratory Practice Standards; Final Rule (40 CFR, Part 160). Federal Register, 48 (230); 34052-34074.



## 6. U.S. EPA, 2002. Short-term methods for estimating the ch ronic toxicity of effluents and receiving waters to freshwater organisms. Third edition. Office of Water, U. S. Environmental Protection Agency, Washington, DC 20460. EPA/82l/R-02/013.



## 7. U.S. EPA, 2012. Office of Chemical Safety and Pollution Prevention. Ecological Effects Test Guideline, OCSPP 850.4150. Vegetative Vigor. EPA 712-C-12-011. January 2012. U.S. Environmental Protection Agency, Washington, D.C.