BASF Plant Science, L.P., Petition (09-015-01p) for 

Determination of Nonregulated Status of HerbicideResistant BASF BPS-CV127-9 Soybean, Event CV127

OECD Unique Identifier: BPS-CV127-9 

Final Environmental Assessment 

January 2014

Agency Contact

Cindy Eck

USDA, APHIS, BRS 

4700 River Road, Unit 91 

Riverdale, MD 20737-1237 

Phone: (301) 734-0667

Fax: (301) 734-8910

Cynthia.A.Eck@aphis.usda.gov

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Mention of companies or commercial products in this report does not imply recommendation or endorsement by the 

U.S. Department of Agriculture over others not mentioned. USDA neither guarantees nor warrants the standard of any 

product mentioned. Product names are mentioned solely to report factually on available data and to provide specific 

information. 

This publication reports research involving pesticides. All uses of pesticides must be registered by appropriate State 

and/or Federal agencies before they can be recommended. 
Final BASF CV127 Soybean EA

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TABLE OF CONTENTS

SECTION........................................................................................................................ PAGE

1 PURPOSE AND NEED.................................................................................................... 8

1.1 BACKGROUND .......................................................................................................... 8

1.2 PURPOSE OF PRODUCT ........................................................................................... 8

1.3 Coordinated Framework Review and Regulatory Review ........................................... 9

1.3.1 USDA-APHIS......................................................................................................... 9

1.3.2 Environmental Protection Agency........................................................................ 10

1.3.3 Food and Drug Administration ............................................................................. 11

1.4 PURPOSE AND NEED FOR USDA-APHIS ACTION............................................ 11

1.5 PUBLIC INVOLVEMENT ........................................................................................ 12

1.5.1 First Opportunity for Public Involvement............................................................. 12

1.5.2 Second Opportunity for Public Involvement ........................................................ 12

1.5.3 Public Comment Period for Petition 09-015-01p ................................................. 13

1.5.4 Public Comment Period for Draft EA for Petition 09-015-01p............................ 14

1.6 ISSUES CONSIDERED............................................................................................. 14

2 ALTERNATIVES........................................................................................................... 16

2.1 NO ACTION: CONTINUATION AS A REGULATED ARTICLE ......................... 16

2.2 PREFERRED ALTERNATIVE: APPROVAL OF THE PETITION FOR 

NONREGULATED STATUS FOR BASF CV127 SOYBEAN............................... 16

2.3 ALTERNATIVES CONSIDERED BUT REJECTED FROM FURTHER 

CONSIDERATION .................................................................................................... 17

2.3.1 Prohibit Any BASF CV127 Soybean from Being Released................................. 17

2.3.2 Approve the Petition In Part ................................................................................. 18

2.3.3 Production/Geographical Restrictions to Isolate BASF CV127 Soybean from 

Non-GE Soybean .................................................................................................. 18

2.3.4 Requirement of Testing for BASF CV127 Soybean............................................. 18

2.4 COMPARISON OF ALTERNATIVES ..................................................................... 19

3 AFFECTED ENVIRONMENT..................................................................................... 24

3.1 Socioeconomics .......................................................................................................... 24

3.1.1 Areas and Acreage of Soybean Production .......................................................... 24

3.1.2 Soybean Seed Production ..................................................................................... 25

3.1.3 Organic Soybean Production ................................................................................ 25

3.1.4 Soybean Imports and Exports............................................................................... 27
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3.2 Human Health and Animal Feed................................................................................. 27

3.2.1 Food and Feed....................................................................................................... 27

3.2.2 Worker Safety ....................................................................................................... 27

3.3 PHYSICAL ENVIRONMENT................................................................................... 28

3.3.1 Soil Quality........................................................................................................... 28

3.3.2 Water Resources ................................................................................................... 28

3.3.3 Air Quality ............................................................................................................ 29

3.3.4 Climate Change..................................................................................................... 29

3.4 BIOLOGICAL RESOURCES.................................................................................... 29

3.4.1 Animals Communities .......................................................................................... 30

3.4.2 Plant Communities................................................................................................ 31

3.4.3 Soil Microorganisms............................................................................................. 31

3.4.4 Biological Diversity.............................................................................................. 31

4 ENVIRONMENTAL CONSEQUENCES.................................................................... 33

4.1 SCOPE OF THE ENVIRONMENTAL ANALYSIS AND ASSUMPTIONS .......... 33

4.2 Socioeconomics .......................................................................................................... 33

4.2.1 No Action - Socioeconomics................................................................................ 33

4.2.2 Preferred Alternative - Socioeconomic Effects .................................................... 40

4.3 Human Health and Animal Feed................................................................................. 42

4.3.1 No Action - Human Health and Animal Feed ...................................................... 42

4.3.2 Preferred Alternative - Human Health and Animal Feed ..................................... 43

4.4 PHYSICAL ENVIRONMENT................................................................................... 44

4.4.1 No Action Alternative – Physical Environment ................................................... 44

4.4.2 Preferred Alternative – Physical Environment ..................................................... 48

4.5 BIOLOGICAL RESOURCES.................................................................................... 49

4.5.1 No Action Alternative – Biological Resources..................................................... 49

4.5.2 Preferred Alternative – Biological Resources....................................................... 56

5 CUMULATIVE IMPACTS ........................................................................................... 56

5.1 Assumptions Used for Cumulative Impacts Analysis ................................................ 56

5.2 Past and Present Actions............................................................................................. 57

5.3 Reasonably Foreseeable Actions ................................................................................ 58
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6 THREATENED AND ENDANGERED SPECIES...................................................... 60

6.1 USDA-APHIS’ APPROACH TO EVALUATION OF POTENTIAL IMPACTS TO 

THREATENED AND ENDANGERED SPECIES ................................................... 60

6.2 SCOPE OF APHIS’ EFFECTS ANALYSIS OF THE CULTIVATION OF BASF 

CV127 SOYBEAN ON TES ...................................................................................... 61

6.3 POTENTIAL EFFECTS OF BASF CV127 SOYBEAN ON TES ........................... 62

7 CONSIDERATION OF EXECUTIVE ORDERS, STANDARDS, AND TREATIES 

RELATING TO ENVIRONMENTAL IMPACTS ..................................................... 65

7.1 EXECUTIVE ORDERS WITH DOMESTIC IMPLICATIONS ............................... 65

7.2 INTERNATIONAL IMPLICATIONS....................................................................... 67

7.3 COMPLIANCE WITH CLEAN WATER ACT AND CLEAN AIR ACT................ 69

7.4 IMPACTS ON UNIQUE CHARACTERISTICS OF GEOGRAPHIC AREAS........ 69

7.5 NATIONAL HISTORIC PRESERVATION ACT (NHPA) OF 1966 AS AMENDED

..................................................................................................................................... 70

8 LIST OF PREPARERS:................................................................................................. 72

9 BIBLIOGRAPHY........................................................................................................... 73

LIST OF TABLES

Table 1: Summary of potential impacts and consequences of alternatives. ................................. 19

Table 2: Certified organic soybean acreage by state. ................................................................... 37

LIST OF FIGURES

Figure 1: Soybean maturity group distributions in the U.S. ........................................................ 24

Figure 2: Soybeans imported to the U.S, 2003-12........................................................................ 38

Figure 3: Countries from which the U.S. imported soybeans in 2012.......................................... 39
Final BASF CV127 Soybean EA

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ACRONYMS AND ABBREVIATIONS

APHIS Animal and Plant Health Inspection Service

AIA advanced informed agreement

AMS Agricultural Marketing Service

AOSCA American Organization of Seed Certifying Agencies

BRS Biotechnology Regulatory Services (within USDA–APHIS)

CAA Clean Air Act

CBD Convention on Biological Diversity

CEQ Council on Environmental Quality

CFR Code of Federal Regulations (United States)

CH4 methane

CO carbon monoxide

CO2 carbon dioxide

CRP Conservation Reserve Program

DNA deoxyribonucleic acid

DRT drift reduction technology

EA environmental assessment

EO Executive Order

EPA U.S. Environmental Protection Agency

ESA Endangered Species Act of 1973

FDA U.S. Food and Drug Administration 

FFDCA Federal Food, Drug, and Cosmetic Act

FFP food, feed, or processing

FIFRA Federal Insecticide, Fungicide, and Rodenticide Act

FONSI Finding of No Significant Impact

FQPA Food Quality Protection Act

FR Federal Register

GE genetically engineered

GHG greenhouse gas

HRAC Herbicide Resistance Action Committee

IPCC Intergovernmental Panel on Climate Change

ISPM International Standard for Phytosanitary Measure

IPPC International Plant Protection Convention

ISO International Organization For Standardization
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ACRONYMS AND ABBREVIATIONS

LMOs Living Modified Organisms

MG Maturity Group

MOA mode of action

MOU Memorandum of Understanding

NO2 nitrogen dioxide

N2O nitrous oxide

NABI North American Biotechnology Initiative

NAPPO North American Plant Protection Organization

NEPA National Environmental Policy Act of 1969 and subsequent amendments

NGO Non-Governmental Organization

NHPA National Historic Preservation Act

NMFS National Marine Fisheries Service

NOI notice of intent

NOP National Organic Program

NPS nonpoint source pollution

NRC National Research Council

OPP Office of Pesticide Program

PIP plant incorporated protectant

PPRA Plant Pest Risk Assessment

PPA Plant Protection Act of 2000

PRA pest risk analysis

RED Registration Eligibility Decision

RNA ribonucleic acid

RSPM Regional Standards for Phytosanitary Measures

SOM soil organic matter

SSA sole source aquifer

TES threatened and endangered species

TSCA Toxic Substances Control Act

U.S. United States

USDA U.S. Department of Agriculture

USDA-APHIS U.S. Department of Agriculture-Animal and Plant Health Inspection 

Service

USDA-ERS U.S. Department of Agriculture-Economic Research Service

USDA-FAS U.S. Department of Agriculture-Foreign Agricultural Service

USDA-NASS U.S. Department of Agriculture-National Agricultural Statistics Service
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ACRONYMS AND ABBREVIATIONS

USDA-NOP U.S. Department of Agriculture-National Organic Program

USC United States Code

WPS Worker Protection Standard for Agricultural Pesticides
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1 PURPOSE AND NEED

1.1 BACKGROUND

BASF Plant Science, L.P. (referred hereafter as BASF) submitted petition number 09-015-01p to 

the United States Department of Agriculture (USDA), Animal and Plant Health Inspection 

Service (APHIS), on July 13, 2009, seeking approval of the petition for nonregulated status for 

BASF CV127 Soybean, a genetically engineered (GE) imidazolinone herbicide-resistant1

soybean (Glycine max) event BPS-CV127-9 (CV127) (referred hereafter as BASF CV127 

Soybean). Field trials of BASF CV127 Soybean have been conducted in Brazil. Data resulting 

from these field trials are described in the BASF petition (BASF, 2011) and analyzed for plant 

pest risk in the USDA-APHIS Plant Pest Risk Assessment (PPRA) (USDA-APHIS, 2012). 

BASF’s petition states that USDA-APHIS should not regulate BASF CV127 Soybean because it 

does not present a plant pest risk (BASF, 2011). In the event of approval of the petition for

nonregulated status, the nonregulated status would include BASF CV127 Soybean, any progeny 

derived from crosses between BASF CV127 Soybean and conventional soybean, and crosses of 

BASF CV127 Soybean with other biotechnology-derived soybean that are no longer subject to 

the regulatory requirements of 7 CFR (Code of Federal Regulations) Part 340 or the plant pest 

provisions of the Plant Protection Act of 2000 (PPA).

1.2 PURPOSE OF PRODUCT

Imidazolinone herbicides2control a wide spectrum of grass and broadleaf weeds (BASF, 2011; 

USDA-APHIS, 2012). Soybeans are naturally resistant to some of the imidazolinone herbicides 

due to an ability to metabolize the compound (Tan et al., 2005). However, soybeans are unable 

to metabolize imazapyr and imazapic, and are thus very sensitive to these imidazolinone

herbicides (BASF, 2011). These two herbicides are active ingredients in a number of 

imidazolinone herbicide products, and imazapyr is found in Lightning®(a mixture of 

imazethapyr – 52.5% and imazapyr – 17.5%;) (BASF, 2008). BASF CV127 Soybean is resistant

to the use of imidazolinone herbicides containing these active ingredients, which provides 

growers with an option for effective weed control using these two herbicides (BASF, 2011).

 

1 Resistance to herbicides is defined by HRAC (Herbicide Resistance Action Committee) as the inherited ability of a 

plant population to survive and reproduce following repeated exposure to a dose of herbicide normally lethal to the 

wild type HRAC, Guideline to the Management of Herbicide Resistance, 2013, Herbicide Resistance Action 

Committee, Available: http://www.hracglobal.com/Publications/ManagementofHerbicideResistance.aspx, January 

22 2013.. Resistance may be induced by genetic engineering or selection of variants produced by tissue culture or 

mutagenesis HRAC, Guideline to the Management of Herbicide Resistance.. This is to be distinguished from 

tolerant, which is defined by HRAC as the inherent ability of a plant to survive and reproduce after herbicide 

treatment. This implies that there was no selection or genetic manipulation to make the plant tolerant; it is naturally 

tolerant HRAC, Guideline to the Management of Herbicide Resistance.. 

2

Imidazolinone herbicides include imazapyr, imazapic, imazethapyr, imazamox, imazamethabenz and imazaquin

Siyuan Tan, Richard R. Evans, Mark L. Dahmer, Bijay K. Singh and Dale L. Shaner, "Imidazolinone-Tolerant 

Crops: History, Current Status and Future," Pest Management Science 61.3 (2005)..
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BASF CV127 Soybean was developed for cultivation primarily in Brazil and Argentina; the 

petitioner indicates that introduction of BASF CV127 Soybean varieties will offer soybean 

growers in Brazil and Argentina an additional tool for controlling weeds, as well as an important 

option for weed resistance management (BASF, 2011; USDA-APHIS, 2012). The use of 

glyphosate with glyphosate-resistant soybeans in Argentina and Brazil has led to a shift in 

prevalent weed species with those more resistant to glyphosate predominating (BASF, 2011).

The major weeds in soybean cultivation in these countries are sensitive to the imidazolinone

herbicides containing imazapyr and imazapic (BASF, 2011). The most common weeds in this 

category include Benghal dayflower (Commelina benghalensis L.), morning glory (Ipomoea 

spp.), Brazil pusley (Richardia brasiliensis), and winged false buttonweed (Spermacoce alata)

(BASF, 2012 ). 

BASF has stated in the petition that it does not intend to commercialize BASF CV127 Soybean 

in the U.S. (BASF, 2011; BASF, 2012 ). Regulatory approvals for CV127 are being sought in 

Brazil and Argentina for production as well as for food and feed uses, and in the U.S. and other 

countries for importation of grain from CV127 soybean for food, feed, and processing uses.

1.3 COORDINATED FRAMEWORK REVIEW AND REGULATORY REVIEW

Since 1986, the U.S. government has regulated genetically engineered (GE) organisms pursuant 

to Federal regulations published in the Federal Register (EOP-OSTP; US-FDA) entitled The 

Coordinated Framework for the Regulation of Biotechnology (henceforth referred to here as the 

Coordinated Framework). The Coordinated Framework, published by the Office of Science and 

Technology Policy, describes the comprehensive federal regulatory policy for ensuring the safety 

of biotechnology research and products and explains how federal agencies will use existing 

Federal statutes in a manner to ensure public health and environmental safety while maintaining 

regulatory flexibility to avoid impeding the growth of the biotechnology industry. The 

Coordinated Framework is based on several important guiding principles: (1) agencies should 

define those transgenic organisms subject to review to the extent permitted by their respective 

statutory authorities; (2) agencies are required to focus on the characteristics and risks of the 

biotechnology product, not the process by which it is created; (3) agencies are mandated to 

exercise oversight of GE organisms only when there is evidence of “unreasonable” risk.

The Coordinated Framework explains the regulatory roles and authorities for the three major 

agencies involved in regulating GE organisms: USDA-APHIS, the U.S. Environmental 

Protection Agency (EPA), and the U.S. Food and Drug Administration (FDA). A summary of 

each agency’s role follows:

1.3.1 USDA-APHIS

USDA-APHIS regulations at 7 CFR part 340, which were promulgated pursuant to authority

granted by the PPA, as amended (7 United States Code (U.S.C.) 7701–7772), regulate the 

introduction (i.e., importation, interstate movement, or release into the environment) of certain 

GE organisms and products. A GE organism is no longer subject to the plant pest provisions of 

the PPA or to the regulatory requirements of 7 CFR part 340, when USDA-APHIS determines 

that it is unlikely to pose a plant pest risk. A GE organism is considered a regulated article if the 

donor organism, recipient organism, vector, or vector agent used in engineering the organism 
Final BASF CV127 Soybean EA

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belongs to one of the taxa listed in the regulation (7 CFR 340.2) and is also considered a plant 

pest. A GE organism is also regulated under 7 CFR 340 when USDA-APHIS has reason to 

believe that the GE organism may be a plant pest or USDA-APHIS does not have information to 

determine if the GE organism is unlikely to pose a plant pest risk. 

A person may petition the agency for a determination that a particular regulated article is 

unlikely to pose a plant pest risk, and therefore, is no longer regulated under the plant pest 

provisions of the PPA or the regulations at 7 CFR 340. Under § 340.6(c)(4), the petitioner must 

provide information related to plant pest risk that the agency can use to determine whether the 

regulated article is unlikely to present a greater plant pest risk than the unmodified organism. A 

GE organism is no longer subject to the regulatory requirements of 7 CFR part 340 or the plant 

pest provisions of the PPA when USDA-APHIS determines that it is unlikely to pose a plant pest 

risk.

1.3.2 Environmental Protection Agency

The EPA is responsible for regulating the sale, distribution, and use of pesticides, including 

pesticides that are produced by an organism through techniques of modern biotechnology. The 

EPA regulates plant incorporated protectants (PIPs) under the Federal Insecticide, Fungicide, and 

Rodenticide Act (FIFRA) (7 U.S.C. 136 et seq.) and certain biological control organisms under 

the Toxic Substances Control Act (TSCA) (15 U.S.C. 53 et seq.). Before planting a crop 

containing a PIP, a company must seek an experimental use permit from EPA. Commercial 

production of crops containing PIPs for purposes of seed increases and sale requires a FIFRA 

Section 3 registration with EPA. 

Under FIFRA (7 U.S.C. 136 et seq.), EPA regulates the use of pesticides, and requires 

registration of all pesticide products for all specific uses prior to distribution for sale. EPA 

examines: the ingredients of the pesticide; the particular site or crop on which it is to be used; the 

amount, frequency, and timing of its use; storage and disposal practices. Prior to registration for 

a new use for a new or previously registered pesticide, EPA must determine through testing that 

the pesticide does not cause unreasonable adverse effects on humans, the environment, and nontarget species when used in accordance with label instructions. EPA must also approve the 

language used on the pesticide label in accordance with 40 CFR part 158. Once registered, a 

pesticide may only be legally used in accordance with directions and restrictions on its label. 

The overall intent of the label is to provide clear directions for effective product performance, 

while minimizing risks to human health and the environment. The Food Quality Protection Act 

(FQPA) of 1996 amended FIFRA, enabling EPA to implement periodic registration review of 

pesticides to ensure they are meeting current scientific and regulatory standards of safety and 

continue to have no unreasonable adverse effects (US-EPA, 2011b). 

EPA also sets tolerances (maximum residue levels) or establishes an exemption from the 

requirement for a tolerance, under the Federal Food, Drug, and Cosmetic Act (FFDCA). A 

tolerance is the amount of pesticide residue that can remain on or in food for human consumption 

or animal feed. Before establishing a pesticide tolerance, EPA is required to reach a safety 

determination based on a finding of reasonable certainty of no harm under the FFDCA, as 

amended by the FQPA. FDA enforces the pesticide tolerances set by EPA.
Final BASF CV127 Soybean EA

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1.3.3 Food and Drug Administration

FDA regulates GE organisms under the authority of the FFDCA (21 U.S.C. 301 et seq.). The 

FDA published its policy statement concerning regulation of products derived from new plant 

varieties, including those derived from genetic engineering, on May 29, 1992 (US-FDA). Under 

this policy, FDA implements a voluntary consultation process to ensure that human food and 

animal feed safety issues or other regulatory issues, such as labeling, are resolved before 

commercial distribution of bioengineered food. This voluntary consultation process provides a 

way for developers to receive assistance from FDA in complying with their obligations under 

Federal food safety laws prior to marketing.

More recently (June 2006), FDA published recommendations in “Guidance for Industry: 

Recommendations for the Early Food Safety Evaluation of New Non-Pesticidal Proteins 

Produced by New Plant Varieties Intended for Food Use” (US-FDA, 2006). This establishes 

voluntary food safety evaluations for new non-pesticidal proteins produced by new plant 

varieties intended to be used as food, including bioengineered plants. Early food safety 

evaluations help make sure that potential food safety issues related to a new protein in a new 

plant variety are addressed early in development. These evaluations are not intended as a 

replacement for a biotechnology consultation with FDA, but the information may be used later in 

the biotechnology consultation.

1.4 PURPOSE AND NEED FOR USDA-APHIS ACTION

As noted in the previous section, any party can petition USDA-APHIS to seek a determination of 

nonregulated status for a GE organism that is regulated under 7 CFR 340. As required by 7 CFR 

340.6, USDA-APHIS must respond to petitioners that request a determination of the regulated 

status of GE organisms, including GE plants such as BASF CV127 Soybean. When a petition 

for nonregulated status is submitted, USDA-APHIS must determine if the GE organism is 

unlikely to pose a plant pest risk. The petitioner is required to provide information under 

§340.6(c)(4) related to plant pest risk that the agency may use to determine whether the regulated 

article is unlikely to present a greater plant pest risk than the unmodified organism. A GE 

organism is no longer subject to the regulatory requirements of 7 CFR part 340 or the plant pest 

provisions of the PPA when APHIS determines that it is unlikely to pose a plant pest risk.

USDA-APHIS must respond to the petition from BASF requesting a determination of 

nonregulated status for BASF CV127 Soybean. USDA-APHIS has prepared this Environmental 

Assessment (EA) to consider the potential environmental effects of an agency determination of 

nonregulated status consistent with Council of Environmental Quality’s (CEQ) National 

Environmental Policy Act of 1969 (NEPA) regulations and the USDA and USDA-APHIS NEPA 

implementing regulations and procedures (40 CFR Parts 1500-1508, 7 CFR Part 1b, and 7 CFR 

Part 372). This EA has been prepared in order to specifically evaluate the effects on the quality

of the human environment1that may result from a determination of nonregulated status for 

BASF CV127 Soybean.

 

1

Under NEPA regulations, the “human environment” includes “the natural and physical environment and the 

relationship of people with that environment” (40 CFR §1508.14).
Final BASF CV127 Soybean EA

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1.5 PUBLIC INVOLVEMENT

USDA-APHIS routinely seeks public comment on EAs prepared in response to petitions seeking 

a determination of nonregulated status of a regulated GE organism. USDA-APHIS does this 

through a notice published in the Federal Register. On March 6, 2012, USDA-APHIS published 

a notice1in the Federal Register advising the public that APHIS is implementing changes to the 

way it solicits public comment when considering petitions for determinations of nonregulated 

status for GE organisms to allow for early public involvement in the process. As identified in this 

notice, USDA-APHIS will publish two separate notices in the Federal Register for petitions for 

which USDA-APHIS prepares an EA. The first notice will announce the availability of the 

petition, and the second notice will announce the availability of USDA-APHIS’ decision making 

documents. As part of the new process, with each of the two notices published in the Federal 

Register, there will be an opportunity for public involvement:

1.5.1 First Opportunity for Public Involvement 

Once USDA-APHIS deems a petition complete, the petition will be made available for public 

comment for 60 days, providing the public an opportunity to raise issues regarding the petition 

itself and give input that will be considered by the Agency as it develops its EA and PPRA.

USDA-APHIS will publish a notice in the Federal Register to inform the public that USDAAPHIS will accept written comments regarding a petition for a determination of nonregulated 

status for a period of 60 days from the date of the notice. This availability of the petition for 

public comment will be announced in a Federal Register notice.

1.5.2 Second Opportunity for Public Involvement 

Assuming an EA is sufficient, the EA and PPRA are developed and a notice of their availability

is published in a second Federal Register notice. This second notice follows one of two 

approaches for public participation based on whether or not USDA-APHIS decides the petition 

for a determination of nonregulated status is for a GE organism that raises substantive new 

issues:

Approach 1: GE organisms that do not raise substantive new issues.

This approach for public participation is used when APHIS decides, based on the review of the 

petition and our evaluation and analysis of comments received from the public during the 60-day

comment period on the petition, that the petition involves a GE organism that does not raise new 

biological, cultural, or ecological issues because of the nature of the modification or APHIS' 

familiarity with the recipient organism. After developing its EA, finding of no significant impact 

(FONSI), and PPRA, USDA-APHIS publishes a notice in the Federal Register announcing its 

preliminary regulatory determination and the availability of the EA, FONSI, and PPRA for a 30-

day public review period.

If USDA-APHIS determines that no substantive information has been received that would 

 

1

 This notice can be accessed at: http://www.gpo.gov/fdsys/pkg/FR-2012-03-06/pdf/2012-5364.pdf
Final BASF CV127 Soybean EA

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warrant USDA-APHIS altering its preliminary regulatory determination or FONSI, substantially 

changing the proposed action identified in the EA, or substantially changing the analysis of 

impacts in the EA, USDA-APHIS’ preliminary regulatory determination becomes final and 

effective upon public notification through an announcement on its website. No further Federal 

Register notice is published announcing the final regulatory determination.

Approach 2: For GE organisms that raise substantive new issues not previously reviewed by 

USDA-APHIS.

A second approach for public participation is used when USDA-APHIS determines that the 

petition for a determination of nonregulated status is for a GE organism that raises substantive 

new issues. This could include petitions involving a recipient organism that has not previously 

been determined by USDA-APHIS to have nonregulated status or when USDA-APHIS 

determines that gene modifications raise substantive biological, cultural, or ecological issues not 

previously analyzed by USDA-APHIS. Substantive issues are identified by APHIS based on our 

review of the petition and our evaluation and analysis of comments received from the public 

during the 60-day comment period on the petition. 

USDA-APHIS solicits comments on its draft EA and draft PPRA for 30 days through the 

publication of a Federal Register notice. USDA-APHIS reviews and evaluates comments and 

other relevant information, then revises the PPRA as necessary and prepares a final EA. 

Following preparation of these documents, USDA-APHIS approves or denies the petition, 

announcing in the Federal Register the regulatory status of the GE organism and the availability

of USDA-APHIS' final EA, PPRA, NEPA decision document (either a FONSI or notice of intent 

(NOI) to prepare an EIS), and regulatory determination.

Enhancements to public input are described in more detail in the Federal Register notice1

published on March 6, 2012.

1.5.3 Public Comment Period for Petition 09-015-01p

USDA-APHIS decided this EA will follow Approach 2 because this is the first EA prepared for 

soybean genetically engineered for resistance to imidazolinone herbicides. The BASF petition 

was published for public comment on July 13, 2012, with comments accepted until September 

11, 2012. A total of 75 public submissions were made to the docket. Some of the submissions to 

the docket contained multiple comments combined together gathered by organizations from their 

members. Counting these individual comments, the 75 submissions contained a total of 4,676

public comments. The majority of the comments expressed a general dislike of the use of GE 

organisms or were form letters sent to all of the dockets which were opened on the same date that 

this docket was opened. The form letter submitted expressed the concern that there were too 

many dockets published on the same day. It also referenced other open dockets and potential 

effects from the use of the subjects of those petitions. These issues are outside the scope of this 

EA.

 

1 This notice can be accessed at: http://www.gpo.gov/fdsys/pkg/FR-2012-03-06/pdf/2012-5364.pdf
Final BASF CV127 Soybean EA

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Issues raised in these public comments on the petition were focused on the nature of agronomic 

inputs associated with this new trait, potential impacts to plants from off-target drift, 

management of herbicide-resistant weeds, human health considerations from exposure to 

herbicides, and domestic and international economic impacts associated with the development 

and marketing of a new herbicide-resistant product. APHIS evaluated these raised issues and the 

submitted documentation. APHIS has also included a discussion of these issues in this EA.

1.5.4 Public Comment Period for Draft EA for Petition 09-015-01p

The draft EA and draft PPRA were made available for public comment during a 30-day

comment period closing on December 9, 2013. Ten comments were received and were carefully

analyzed to identify new issues, alternatives, or information. The public comments in response 

to the petition and the EA may be viewed at the federal website, regulations.gov1. APHIS 

evaluated the issues raised and the submitted documentation. APHIS has included a discussion of 

issues relative to this petition in the EA or in the response to comments attached to the National 

Environmental Policy Act Decision and Finding of No Significant Impact document, posted at the 

federal website, regulations.gov. 

1.6 ISSUES CONSIDERED

The list of resource areas considered in this EA were developed by USDA-APHIS through 

experience in considering public concerns and issues raised in public comments submitted for 

this petition and other EAs of GE organisms. The resource areas considered also address 

concerns raised in previous and unrelated lawsuits, as well as issues that have been raised by 

various stakeholders for this petition and in the past. The resource areas considered in this EA 

can be categorized as follows: 

Agricultural Production Considerations

 Acreage and Areas of Soybean Production

 Soybean Seed Production

 Organic Soybean Production

Environmental Considerations

 Soil Quality

 Water Resources

 Air Quality

 Climate Change

 Animal Communities

 Plant Communities

 Soil Microorganisms

 Biological Diversity

Human Health Considerations

 

1

http://www.regulations.gov/#!searchResults;rpp=50;so=ASC;sb=docId;po=0;s=APHIS-2012-0046
Final BASF CV127 Soybean EA

15

 Public Health

 Worker Safety

Livestock Health Considerations

 Animal Feed/Livestock Health

Socioeconomic Considerations

 Domestic Economics

 Trade Economics 
Final BASF CV127 Soybean EA

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2 ALTERNATIVES

This document analyzes the potential environmental consequences of the approval of the petition 

for nonregulated status of BASF CV127 Soybean. To respond favorably to a petition for 

nonregulated status, USDA-APHIS must determine that BASF CV127 Soybean is unlikely to 

pose a plant pest risk. Based on its PPRA (USDA-APHIS, 2012), USDA-APHIS has concluded 

that BASF CV127 Soybean is unlikely to pose a plant pest risk. Therefore, USDA-APHIS must 

determine that BASF CV127 Soybean is no longer subject to 7 CFR Part 340 or the plant pest 

provisions of the PPA. 

Two alternatives will be evaluated in this EA: 1) No Action and 2) approval of a petition for 

nonregulated status of BASF CV127 Soybean. USDA-APHIS has assessed the potential for 

environmental impacts for each alternative in the Environmental Consequences section.

2.1 NO ACTION: CONTINUATION AS A REGULATED ARTICLE

Under the No Action Alternative, USDA-APHIS would deny the petition. BASF CV127 

Soybean and progeny derived from BASF CV127 Soybean would continue to be regulated 

articles under the regulations at 7 CFR Part 340. Permits issued or notifications acknowledged 

by USDA-APHIS would still be required for introductions of BASF CV127 Soybean and 

measures to ensure physical and reproductive confinement would continue to be implemented.

USDA-APHIS might choose this alternative if there were insufficient evidence to demonstrate 

the lack of plant pest risk from the unconfined cultivation of BASF CV127 Soybean. 

However, this alternative is not the Preferred Alternative because USDA-APHIS has concluded 

through a PPRA that BASF CV127 Soybean is unlikely to pose a plant pest risk (USDA-APHIS, 

2012). Choosing this alternative would not satisfy the purpose and need of making a 

determination of plant pest risk status and responding to the petition for nonregulated status.

2.2 PREFERRED ALTERNATIVE: APPROVAL OF THE PETITION FOR NONREGULATED 

STATUS FOR BASF CV127 SOYBEAN

Under this alternative, BASF CV127 Soybean and progeny derived from them would no longer 

be regulated articles under the regulations at 7 CFR Part 340. Permits issued or notifications 

acknowledged by USDA-APHIS would no longer be required for introductions of BASF CV127 

Soybean and progeny derived from this event. 

This alternative best meets the purpose and need to respond appropriately to a petition for 

nonregulated status based on the requirements in 7 CFR Part 340 and the agency’s authority

under the plant pest provisions of the PPA. Because the agency has concluded that BASF CV127 

Soybean is unlikely to pose a plant pest risk, approval of a petition for nonregulated status of 

BASF CV127 Soybean is a response that is consistent with the plant pest provisions of the PPA, 

the regulations codified in 7 CFR Part 340, and the biotechnology regulatory policies in the 

Coordinated Framework. 

Under this alternative, BASF CV127 Soybean could be developed for its target markets outside 

of the U.S. Breeders in the U.S. would no longer require permits to be issued or notifications to 

be acknowledged to conduct outdoor breeding activities. BASF CV127 Soybean could be 
Final BASF CV127 Soybean EA

17

imported into the U.S. from other markets without a permit. If soybean varieties are marketed in 

the U.S. which contain this trait, growers could plant them without APHIS permits. Application 

of herbicides to this and all soybeans is regulated by EPA. Currently, imazapyr is not labeled for 

use on CV127 Soybean and the developer is not seeking a label for its application in the U.S. As 

stated in the petition, this soybean is not intended to be marketed for planting in the U.S. 

Growers and other parties that are involved in production, handling, processing, or consumption 

of soybean would continue to be able to use the current soybean products developed by 

conventional breeding as well as the GE soybean variety. 

2.3 ALTERNATIVES CONSIDERED BUT REJECTED FROM FURTHER CONSIDERATION

USDA-APHIS assembled a comprehensive list of alternatives that might be considered for 

BASF CV127 Soybean. The agency evaluated these alternatives in accordance with its authority

under the plant pest provisions of the PPA, and the regulations at 7 CFR Part 340. The evaluation 

considered environmental safety, efficacy, and practicality to identify which alternatives would 

be further considered for BASF CV127 Soybean. Based on this evaluation, USDA-APHIS

rejected several alternatives. These alternatives are discussed briefly below along with the 

specific reasons for rejecting each.

2.3.1 Prohibit Any BASF CV127 Soybean from Being Released

In response to public comments that might state a preference that no GE organisms enter the 

marketplace, USDA-APHIS considered prohibiting the release of BASF CV127 Soybean, 

including denying any permits associated with the field testing. USDA-APHIS determined 

that this alternative is not appropriate given that BASF CV127 Soybean is unlikely to pose a 

plant pest risk (USDA-APHIS, 2012). 

In enacting the PPA, Congress found that—

[D]ecisions affecting imports, exports, and interstate movement of products 

regulated under [the PPA] shall be based on sound science...§402(4) (codified at 

7 U.S.C. §7701(4)).

On March 11, 2011, in a Memorandum for the Heads of Executive Departments and Agencies, 

the White House Emerging Technologies Interagency Policy Coordination Committee 

established broad principles, consistent with Executive Order 13563, to guide agencies in the 

development and implementation of policies for oversight of emerging technologies such as GE 

that included the following guidance:

[D]ecisions should be based on the best reasonably obtainable scientific, 

technical, economic, and other information, within the boundaries of the 

authorities and mandates of each agency. 

Consistent with this guidance and based on the findings and scientific data evaluated for the 

PPRA (USDA-APHIS, 2012), USDA-APHIS concluded that BASF CV127 Soybean is not 

likely to present a plant pest risk. Accordingly, there is no basis in science for prohibiting 

the release of BASF CV127 Soybean. 
Final BASF CV127 Soybean EA

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2.3.2 Approve the Petition In Part

The regulations at 7 CFR 340.6(d)(3)(i) state that USDA-APHIS may "approve the petition in 

whole or in part." For example, approving the petition for nonregulated status in part may be 

appropriate if there is a plant pest risk associated with some, but not all lines described in a 

petition. USDA-APHIS has concluded that BASF CV127 Soybean is unlikely to pose a plant 

pest risk, so there is no regulatory basis under the plant pest provisions of the PPA for 

considering approval of the petition only in part.

2.3.3 Production/Geographical Restrictions to Isolate BASF CV127 Soybean from NonGE Soybean 

In response to public concerns of gene movement between GE and non-GE plants, USDAAPHIS considered requiring an isolation distance of BASF CV127 Soybean from non-GE 

soybean production. However, because USDA-APHIS has concluded that BASF CV127 Soybean

is unlikely to pose a plant pest risk (USDA-APHIS, 2012), an alternative based on requiring 

isolation distances would be inconsistent with the statutory authority under the plant pest 

provisions of the PPA and regulations in 7 CFR part 340.

USDA-APHIS also considered geographically restricting the production of BASF CV127 

Soybean based on the location of production of non-GE soybean in organic production systems 

in response to public concerns regarding possible gene movement between GE and non-GE 

plants. However, as presented in USDA-APHIS’ PPRA for BASF CV127 Soybean, there are no 

geographic differences associated with any identifiable plant pest risks for BASF CV127 

Soybean (USDA-APHIS, 2012). This alternative was rejected and not analyzed in detail because 

USDA-APHIS has concluded that BASF CV127 Soybean does not pose a plant pest risk, and 

will not exhibit a greater plant pest risk in any geographically restricted area (USDA-APHIS, 

2012). Therefore, such an alternative would not be consistent with USDA-APHIS’ statutory

authority under the plant pest provisions of the PPA and regulations in Part 340 and the 

biotechnology regulatory policies embodied in the Coordinated Framework. 

Based on the foregoing considerations, the imposition of isolation distances or geographic 

restrictions would not meet USDA-APHIS’ purpose and need to respond appropriately to a 

petition for nonregulated status based on the requirements in 7 CFR part 340 and the agency‘s 

authority under the plant pest provisions of the PPA. However, individuals might choose on their 

own to geographically isolate their non-GE soybean productions systems from soybean 

incorporating the BASF CV127 Soybean event or to use isolation distances and other 

management practices to minimize gene movement between soybean fields. Information to assist 

growers in making informed management decisions for BASF CV127 Soybean is available from 

Association of Official Seed Certifying Agencies (AOSCA, 2009).

2.3.4 Requirement of Testing for BASF CV127 Soybean

During the comment periods for other petitions for approving nonregulated status, some 

commenters requested that USDA require and provide testing for GE products in non-GE 

production systems. However, because BASF CV127 Soybean does not pose a plant pest risk 

(USDA-APHIS, 2012), the imposition of any type of testing requirements is inconsistent with 

the plant pest provisions of the PPA, the regulations at 7 CFR part 340, and the biotechnology
Final BASF CV127 Soybean EA

19

regulatory policies embodied in the Coordinated Framework. Therefore, imposing such a 

requirement for BASF CV127 Soybean would not meet USDA-APHIS’ purpose and need to 

respond appropriately to the petition in accordance with its regulatory authorities.

2.4 COMPARISON OF ALTERNATIVES

Table 1 presents a summary of the potential impacts associated with selection of either of the 

alternatives evaluated in this EA. The impact assessment is presented in Section 4 of this EA.

Table 1: Summary of potential impacts and consequences of alternatives.

Attribute/Measure

Alternative A:

No Action

Alternative B:

Nonregulation in Whole

Meets Purpose and 

Need and Objectives No Yes

Unlikely to pose a 

plant pest risk Satisfied by regulated field trials Satisfied – risk assessment(USDA-APHIS, 2012)

Management Practices

Acreage and Areas of 

Soybean Production

In 2012, soybean was cultivated on over 75 

million acres (USDA-NASS, 2012a). 

Approximately 93% of U.S. soybean acreage 

is planted with GE soybean (USDA-ERS, 

2012a; USDA-NASS, 2012a). Acreage 

dedicated to soybean production is expected 

to continue within the 10-year average of 73.3 

million acres.

No change from 

Alternative A

Soybean Seed 

Production

Soybean seed is produced throughout most of 

the U.S. soybean-growing regions (BASF, 

2012 ). About 71.8 million acres were planted 

with certified seeds, and an estimated 1.3 to 

2.7 million tons of certified soybean planting 

seeds were required in 2012 (USDA-ERS, 

2012b).

No change from 

Alternative A 

Organic Soybean 

Production

Organic soybean production is occurring in 

the presence of conventional soybean 

production using GE and non-GE soybean 

varieties, and representing 0.13 to 0.17% of 

total acreage.

No change from 

Alternative A 

Environment

Soil Quality 

Several concerns relating to agricultural 

practices include increased erosion, soil 

compaction, degradation of soil structure, 

nutrient loss, increased salinity, change in pH, 

and reduced biological activity (USDANRCS, 2006c).

No change from 

Alternative A 

Water Resources

Agricultural non-point sources from soybean 

production may derive from many different 

agronomic inputs, including fertilizer and 

pesticide application, and spilled oil and 

gasoline from farm equipment. Agricultural 

No change from 

Alternative A 
Final BASF CV127 Soybean EA

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Attribute/Measure

Alternative A:

No Action

Alternative B:

Nonregulation in Whole

NPS pollution is the leading source of impacts 

to surveyed rivers and lakes and the third 

largest source of impairment to estuaries, as 

well as a major source of impairment to 

groundwater and wetlands (US-EPA, 1996).

Air Quality

Agricultural production of soybean has the

potential to cause negative impacts to air 

quality. Agricultural emission sources 

include smoke from agricultural burning, 

tillage, heavy equipment emissions, pesticide 

drift from spraying, indirect emissions of 

carbon dioxide and nitrous oxide, the 

degradation of organic materials in the soil, 

and from the use of nitrogen fertilizer 

(USDA-NRCS, 2006a; Aneja et al., 2009).

No change from 

Alternative A 

Climate Change

Agriculture is responsible for an estimated 6% 

of all human-induced GHG emissions in the 

United States, produced through the 

combustion of fossil fuels to run farm

equipment; the use of fertilizers; from 

bringing new lands into production; and the 

decomposition of agricultural waste products 

including crop residues, animal wastes, and 

enteric emissions from livestock (US-EPA, 

2012a) .

No change from 

Alternative A 

Animal Communities

During the spring and summer months, 

soybean fields provide browse for rabbits, 

deer, rodents, other mammals; birds such as 

upland gamebirds, while also providing a 

forage base for insects (Palmer et al., No 

Date). During the winter months, leftover and 

unharvested soybeans provide a food-source 

for wildlife (Krapu et al., 2004). Insects and 

other invertebrates can be beneficial to 

soybean production, providing services such 

as nutrient cycling and preying on plant pests. 

Conversely, there are many insects and 

invertebrates that are detrimental to soybean 

crops (Whitworth et al., 2011; Palmer et al., 

No Date). 

No change from 

Alternative A 

Plant Communities

Non-crop vegetation in soybean fields is 

limited by the extensive weed control 

programs using mechanical and chemical 

methods. Volunteer soybean is not a 

widespread problem, and when they occur, it 

is most often in parts of the Delta and the 

southeastern United States. In production 

systems where soybean is rotated, such as 

No change from 

Alternative A 
Final BASF CV127 Soybean EA

21

Attribute/Measure

Alternative A:

No Action

Alternative B:

Nonregulation in Whole

corn or cotton, it has shown up as a volunteer 

weed, yet was not generally seen as a serious 

problem by farmers (Owen and Zelaya, 2005). 

The cultivated soybean, G. max, lacks 

sexually compatible wild relatives in the 

United States and its territories (USDAAPHIS, 2012).

Soil Microorganisms

An important group of soil microorganisms 

associated with legumes, including soybean, 

are the mutualists. These include mycorrhizal 

fungi, nitrogen-fixing bacteria, and some freeliving microbes that have co-evolved with 

plants that supply nutrients to and obtain food 

from their plant hosts (USDA-NRCS, 2004). 

In addition to beneficial microorganisms, 

there are also several microbial pathogens that 

cause disease in soybean and vary somewhat 

depending on the region (Ruhl, 2007; SSDW, 

No Date). 

No change from 

Alternative A 

Biological Diversity

An increase in adoption of conservation 

tillage practices is associated with the use of 

GE herbicide-resistant soybeans (Givens et 

al., 2009). Less tillage provides more wildlife 

habitat by allowing other plants to establish 

between crop rows in either stubble or weeds. 

Conservation tillage also leaves a higher rate 

of plant residue and increases soil organic 

matter (Hussain et al., 1999), which benefit 

soil biota by providing additional food 

sources (energy) and increase the diversity of 

soil microorganisms (USDA-NRCS, 1996).

No change from 

Alternative A 

Human Health and Animal Feed

Human Health

The general population of the U.S. is most 

likely to consume soybean products or 

consume foods containing or prepared with 

soybean oil. Soybeans yield both solid (meal) 

and liquid (oil) products. Soybean meal is 

high in protein and is used for products such 

as tofu, soymilk, meat replacements, and 

protein powder; it also provides a natural 

source of dietary fiber (USB, 2009). Less 

than 2% of soybean meal produced in the 

United States is used to produce soy flour and 

proteins for food use (Soyatech, 2011). 

Extracted soybean liquid oils are used to 

produce salad and cooking oils, baking and 

frying fat, and margarine. Soy oil is low in 

saturated fats, high in poly and 

No change from 

Alternative A 
Final BASF CV127 Soybean EA

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Attribute/Measure

Alternative A:

No Action

Alternative B:

Nonregulation in Whole

monounsaturated fats, and contains essential 

omega-3 fatty acids. Soybean oil comprises 

nearly 70% of the oils consumed in U.S. 

households (ASA, 2010).

Risk to Worker Safety

Agronomic practices used for soybean 

production include the application of 

agricultural chemicals (pesticides and 

fertilizers). Worker safety is taken into 

consideration by EPA in the pesticide 

registration process and reregistration process. 

Pesticides are regularly reevaluated by EPA 

for each pesticide to maintain its registered 

status under FIFRA. Furthermore, the OSHA 

requires all employers to protect their 

employees from hazards associated with 

pesticides and herbicides. When used 

according to label directions, pesticides 

present minimal risk to human health.

No change from 

Alternative A 

Risk to Animal Feed

Animal agriculture consumes 98% of the U.S. 

soybean meal produced (Soyatech, 2011) and 

70% of soybeans worldwide (USB, 2011). 

Poultry consume more than 48% of domestic 

soybean meal or 11.92 million MT of the U.S. 

soybean crop, with soy oil increasingly 

replacing animal fats and oils in broiler diets 

(USB, 2011; ASA, 2012). Soybean can be the 

dominant component of livestock diets, such 

as in poultry, where upwards of 66% of their 

protein intake is derived from soy (Waldroup 

and Smith, No Date). Other animals fed 

domestic soybean by crop volumes consumed 

include swine (26%), beef cattle (12%), dairy 

cattle (9%), other (e.g., farm-raised fish 3%), 

and household pets (3%) (USB, 2009; ASA, 

2010).

No change from 

Alternative A

Socioeconomics

Domestic Economic

Environment

In 2011, 77 million acres of soybeans were 

planted in the U.S., yielding 3.1 billion 

bushels (84.4 MMT) at a value of 35.8 billion 

U.S. dollars (USDA-NASS, 2012f). In the 20 

years from 1991 to 2011, U.S. soybean 

acreage expanded 31% and yields increased 

17.6% (USDA-NASS, 2012f) while annual 

value of production increased approximately 

222% from approximately $11.09 billion to 

approximately $35.8 billion (USDA-NASS, 

2012f). 

No change from 

Alternative A 
Final BASF CV127 Soybean EA

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Attribute/Measure

Alternative A:

No Action

Alternative B:

Nonregulation in Whole

Trade Economic

Environment

The U.S. is the world’s largest exporter of 

soybeans, accounting for 41% of global 

soybean oilseed exports in 2011-12 (USDAFAS, 2012c; USDA-FAS, 2012b). In 2011-

12, U.S. exports of soybeans, soybean cake 

and meal and soybean oil totaled over $22 

billion (USDA-FAS, 2012a). China is the 

largest importer of U.S. soybeans and soybean 

products, accounting for 48% of the total 

value of U.S. soybean and soybean product 

exports, followed by Mexico (8.3% of total) 

and Japan (5.2%) (USDA-FAS, 2012a). The 

U.S. has been importing increasing quantities 

of soybeans over the past decade. Canada 

provides the majority of these soybeans to 

U.S. market. In 2012, approximately 6% of 

the total soybean imports were from South 

America.

No change from 

Alternative A

Other Regulatory Approvals

United States Federal 

Agencies

EPA: BASF does not intend to seek a change 

to the pesticide label or pesticide residue 

tolerances. BASF has applied for an import 

residue tolerance.

FDA: BASF has received a completed 

consultation letter from the FDA

No change from 

Alternative A

Other Country 

Approvals

BASF has submitted applications to Brazil 

and Argentina for approval for cultivation as 

well as for consumption as food or feed. 

Approval of the Brazil National Technical 

Committee for Biosafety was obtained in 

December 2009, and from the Secretariat of 

Agriculture, Livestock and Fisheries of the 

Argentine Republic in March 2013. 

Approvals for food or feed uses have also 

been obtained in countries that import 

significant numbers of soybeans including 

Japan, Canada, the People’s Republic of 

China, Australia, New Zealand, the Republic 

of China (Taiwan), the Republic of Korea, the 

Philippines, Mexico, Columbia, South Africa, 

and the Russian Federation (McKean, 2013).

No change from 

Alternative A

Compliance with Other Laws

CWA, CAA1, EOs Fully compliant Fully compliant

Notes:

CAA – Clean Air Act; CWA – Clean Water Act
Final BASF CV127 Soybean EA

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3 AFFECTED ENVIRONMENT

3.1 SOCIOECONOMICS

3.1.1 Areas and Acreage of Soybean Production 

In the U.S. there are 310 million acres of harvested cropland (USDA-NASS, 2009). Soybean is 

grown as a commercial crop on over 75 million acres in the U.S. Soybean is grown on 24% of 

the harvested cropland. It is the second most frequently planted crop in the U.S. 

http://www.ers.usda.gov/topics/crops/soybeans-oil-crops/background.aspx. About 80% of the 

soybeans are grown in the upper Midwest. http://www.ers.usda.gov/topics/crops/soybeans-oilcrops/background.aspx

Soybean varieties differ in their response and tolerance to temperatures as well as photoperiod. 

Soybean is a quantitative short-day plant, flowering more quickly under short days (OECD, 

2000). As a result, photoperiod and temperature responses are important in determining areas of

specific cultivar adaptation. Soybean cultivars are identified based on geographic bands of 

adaptation that run east-west, determined by latitude and day length (Zhang et al., 2007; Pioneer, 

2012). In North America, there are 13 maturity groups (MGs) described, ranging from MG 000 

in the north (45 degrees [°] latitude) to MG X near the equator.

Figure 1: Soybean maturity group distributions in the U.S.

Source: (Zhang et al., 2007)

1

.

 

1 Zhang et al. L. X. Zhang, S. Kyei-Boahen, J. Zhang, M.H. Zhang, T.B. Freeland, C.E. Watson Jr. and X. Liu, 

"Modifications of Optimum Adaptation Zones for Soybean Maturity Groups in the USA," Crop Management 

(2007).present proposed new soybean maturity group zones, identifying seven maturity groups in the continental 

United States rather than the ten more commonly considered. The proposed new maturity group designations 

combine several of the historically accepted maturity group zones. In the Zhang model, soybean maturity group VIII 

is now identified as maturity group VI, covering the same area and region. Because most of the literature continues 

to refer to the traditional ten maturity groups, these designations are retained in this EA.
Final BASF CV127 Soybean EA

25

Within each maturity group, cultivars are described as early, medium, or late maturing (OECD, 

2000). Figure 1 shows the ten soybean maturity groups commonly associated with the U.S. 

Generally, Groups 00 through IV soybean varieties are planted in the Midwest and Eastern 

Coastal regions; Groups IV through VIII are planted in the southern states (Hodges and French, 

1985; Helsel and Minor, 1993). 

3.1.2 Soybean Seed Production

Seed quality (including genetic purity, vigor, and presence of weed seed, seed-borne diseases, 

and inert materials such as dirt) is a major factor in crop yields. If natural variability in seed 

production is not carefully controlled, the value of a new variety or cultivar may be lost 

(Hartman and Kester, 1975). Genetic purity in commercial seed production is generally 

regulated through a system of seed certification which is intended to ensure that the desired traits 

in the seed are maintained throughout all stages in cultivation (Hartman and Kester, 1975). 

States have developed seed laws and certification agencies to ensure that purchasers who 

received certified seed can be assured that the seed meets established seed quality standards 

(Bradford, 2006). The U.S. Federal Seed Act of 1939 recognizes seed certification and official

certifying agencies. Implementing regulations further recognize land history, field isolation, and 

varietal purity standards for Foundation, Registered, and Certified seed. 

Soybean seed is produced throughout most of the U.S. soybean-growing regions by companies 

that produce and sell seed, and by toll seed producers, or tollers, which produce certified seed for 

other companies (BASF, 2012 ). Seed production or processing plants clean, condition, and bag 

the harvested soybean seed as well as monitor and inspect all the processes at the plant under 

standards established by the International Organization for Standardization (ISO), and includes 

internal and external audit (ISO, 2009). Field inspections are conducted on seed production 

fields throughout the soybean growing season to evaluate variety purity and ensure soybean 

plants are developing properly (AOSCA, 2009).

3.1.3 Organic Soybean Production

In the U.S., only products produced using specific methods and certified under the USDA’s 

Agricultural Marketing Service (AMS) National Organic Program (NOP) definition of organic 

farming can be marketed and labeled as “organic” (USDA-AMS, 2012). Organic 

certification is a process-based certification, not a certification of the end product; the 

certification process specifies and audits the methods and procedures by which the product 

is produced.

In accordance with NOP, an accredited organic certifying agent conducts an annual review of the 

certified operation’s organic system plan and makes on-site inspections of the certified operation 

and its records. Organic growers must maintain records to show that production and handling 

procedures comply with USDA organic standards. 

The NOP regulations preclude the use of excluded methods. The NOP provides the following 

guidance under 7 CFR §205.105—

 
Final BASF CV127 Soybean EA

26

...to be sold or labeled as “100 percent organic”, “organic” or “made with organic 

(specified ingredients or group(s)),” the product must be produced and handled without 

the use of:...

(a) Synthetic substances and ingredients,...

(e) Excluded methods,...

Excluded methods are then defined at 7 CFR §205.2 as—

A variety of methods used to genetically modify organisms or influence their growth 

and development by means that are not possible under natural conditions or processes 

and are not considered compatible with organic production. Such methods include cell 

fusion, microencapsulation and macroencapsulation, and recombinant DNA technology 

(including gene deletion, gene doubling, introducing a foreign gene, and changing the 

positions of genes when achieved by recombinant DNA technology). Such methods do 

not include the use of traditional breeding, conjugation, fermentation, hybridization, in 

vitro fertilization, or tissue culture.

Organic production operations must develop and maintain an organic production system plan 

approved by their accredited certifying agent. This plan enables the production operation to 

achieve and document compliance with the National Organic Standards, including the 

prohibition on the use of excluded methods. In NOP organic systems, the use of synthetic 

pesticides or fertilizers, and GE crops, is strictly limited. Common organic soybean production 

practices include crop rotation, use of cover crops, green and animal manures, application of 

rock minerals such as lime, other soil additives, mechanical weed control, biological control of 

pests, and disease control primarily through management practices (Kuepper, 2003; Heatherly et 

al., 2005; USB, 2011; USDA-AMS, 2012).

The NOP also requires organic farming operations to have distinct, defined boundaries and 

buffer zones to prevent unintended contact with excluded methods from adjoining land that is not 

under organic management (USDA-AMS, 2012). Management practices organic growers may 

use to exclude GE products include planting only organic seed, planting earlier or later than 

neighboring farmers who may be using GE crops so that the crops will flower at different 

times, and employing adequate isolation distances between the organic fields and the fields 

of neighbors to minimize the chance that pollen will be carried between the fields (NCAT, 

2003).

Although the National Organic Standards prohibit the use of excluded methods, they do not 

require testing of inputs or products for the presence of excluded methods. The presence of a 

detectable residue of a product of excluded methods alone does not necessarily constitute a 

violation of the National Organic Standards (USDA-AMS, 2011). The current NOP regulations 

do not specify an acceptable threshold level for the adventitious presence of genetically 

engineered materials in an organic-labeled product. The unintentional presence of the products 

of excluded methods will not affect the status of an organic product or operation when the 

operation has not used excluded methods and has taken reasonable steps to avoid contact with 

the products of excluded methods as detailed in their approved organic system plan. (Ronald and 

Fouche, 2006; USDA-AMS, 2011; USDA-AMS, 2012). 
Final BASF CV127 Soybean EA

27

3.1.4 Soybean Imports and Exports

The U.S. is the world’s largest producer and exporter of soybeans. Main export destinations for 

U.S. oilseeds, oilseed meal, and vegetable oil include China, the European Union (EU), Japan, 

Mexico, and Taiwan. Other important markets also import significant quantities of U.S. oilseed 

meals. These include: Indonesia, South Korea, Thailand, Canada, Mexico, the Philippines, and 

several Latin American countries. (http://www.ers.usda.gov/topics/crops/soybeans-oilcrops/trade.aspx) 

The U.S. imports a limited quantity of soybeans with the majority coming from Canada (GATS 

database, see http://apps.fas.usda.gov/gats/ExpressQuery1.aspx). Currently less than 10% of the 

imported soybeans come from South America (GATS database).

3.2 HUMAN HEALTH AND ANIMAL FEED

3.2.1 Food and Feed

Humans and animals consume soybeans products made from soybeans. Soybeans yield both 

solid (meal) and liquid (oil) products. Soybean meal is high in protein and is used for products 

such as tofu, soymilk, meat replacements, and protein powder; it also provides a natural source 

of dietary fiber (USB, 2009). Nearly 98% of soybean meal produced in the United States is used 

as animal feed, while less than 2% is used to produce soy flour and proteins for food use 

(Soyatech, 2011). Extracted soybean liquid oils are used to produce salad and cooking oils, 

baking and frying fat, and margarine. Soy oil is low in saturated fats, high in poly and 

monounsaturated fats, and contains essential omega-3 fatty acids. Soybean oil comprises nearly 

70% of the oils consumed in U.S. households (ASA, 2010).

3.2.2 Worker Safety

Agriculture is one of the most hazardous industries for U.S. workers. As a result, Congress 

directed the National Institute of Occupational Safety and Health to develop a program to 

address high-risk issues related to occupational workers. In consideration of the risk of pesticide 

exposure to field workers, EPA’s Worker Protection Standard (WPS) (40 CFR Part170) was 

published in 1992 to require actions to reduce the risk of pesticide poisonings and injuries among 

agricultural workers and pesticide handlers. The WPS offers protections to more than two and a 

half million agricultural workers who work with pesticides at more than 560,000 workplaces on 

farms, forests, nurseries, and greenhouses. The WPS contains requirements for pesticide safety 

training, notification of pesticide applications, use of personal protective equipment, restricted 

entry intervals following pesticide application, decontamination supplies, and emergency

medical assistance; furthermore, the Occupational Safety and Health Administration (OSHA) 

require all employers to protect their employees from hazards associated with pesticides and 

herbicides.

Pesticides, which include herbicides, are used on most soybean acreage in the U.S., and changes 

in acreage, crops, or farming practices can affect the amounts and types of pesticides used and 

thus the potential risks to farm workers. The EPA pesticide registration process, however, 

involves the design of use restrictions that, if followed, have been determined to be protective of 

worker health. Under FIFRA, all pesticides, (which is inclusive of herbicides) sold or distributed 

in the U.S. must be registered by the EPA (US-EPA, 2005c). Registration decisions are based on 
Final BASF CV127 Soybean EA

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scientific studies that assess the chemical’s potential toxicity and environmental impact. To be 

registered, a pesticide must be able to be used without posing unreasonable risks to people or the 

environment. All pesticides registered prior to November 1, 1984 must also be reregistered to 

ensure that they meet the current, more stringent standards. During the registration decision, the 

EPA must find that a pesticide does not cause unreasonable adverse effects to human health or 

the environment if used in accordance with the approved label instructions (OSTP, 2001).

3.3 PHYSICAL ENVIRONMENT

3.3.1 Soil Quality

Soil consists of solids (minerals and organic matter), liquids, and gases. This body of inorganic 

and organic matter is home to a wide variety of fungi, bacteria, and arthropods, as well as the 

growth medium for terrestrial plant life (USDA-NRCS, 2004). The soil microorganisms play a 

key role in soil structure (Garbeva et al., 2004); (Young and Ritz, 2000). This soil area in the 

root zone, the rhizosphere, is discussed in Subsection 4.5.1, Biological Resources: No Action 

Alternative – Soil Microorganisms and Soybeans.

Soybean fields are typically highly managed agricultural areas that are dedicated to crop 

production for many years. Soybeans are normally grown in managed agricultural fields for crop 

production and are best suited to fertile, well-drained medium-textured loam soils, yet can be 

produced in a wide range of soil types (Berglund and Helms, 2003; NSRL, 2012), although 

soybean does not do well in acid soils (OECD, 2000). 

Soybeans need a variety of macronutrients, such as nitrogen, phosphorus, potassium, calcium, 

magnesium, and sulfur, at various levels (NSRL, 2012). They also require smaller amounts of 

micronutrients such as iron, zinc, copper, boron, manganese, molybdenum, cobalt, and chlorine. 

These micronutrients may be deficient in poor, weathered soils, sandy soils, alkaline soils, or 

soils excessively high in organic matter. As with proper nutrient levels, soil pH is critical for 

soybean development. Soybeans grow best in soil that is slightly acidic (pH 5.8 to 7.0), and soils 

that are high in clay and low in humus may impede plant emergence and development (NSRL, 

2012). 

3.3.2 Water Resources

Water resources can be partitioned into surface water, groundwater, and runoff. Surface water in 

rivers, streams, creeks, lakes, and reservoirs supports everyday life through the provision of 

water for drinking and other public uses, irrigation, and industry. Surface runoff from rain, 

snowmelt, or irrigation water can affect surface water quality by depositing sediment, minerals, 

or contaminants into surface water bodies (US-EPA, 2005b). Surface runoff is influenced by

meteorological factors such as rainfall intensity and duration, and physical factors such as 

vegetation, soil type, and topography (US-EPA, 2003). 

Groundwater is the water that flows underground and is stored in natural geologic formations

called aquifers; it sustains ecosystems by releasing a constant supply of water into wetlands and 

contributes a sizeable amount of flow to permanent streams and rivers (US-EPA, 1990). Based 

on 2005 data, the largest use of groundwater in the U.S. is irrigation, representing approximately

67.2% of all the groundwater pumped [withdrawn] each day (McCray, 2012). In the U.S., 
Final BASF CV127 Soybean EA

29

approximately 47% of the population depends on groundwater for its drinking water supply. The 

US-EPA defines a sole source aquifer (SSA) as an aquifer that supplies at least 50% of the 

drinking water consumed in the area overlying the aquifer. An SSA designation is one tool to 

protect drinking water supplies in areas where there are few or no alternative sources to the 

groundwater resource. There are 77 designated SSAs in the U.S. and its territories (US-EPA, 

2012b).

3.3.3 Air Quality

Dry air consists of about 78% nitrogen, 21% oxygen, 0.9% argon and 0.03% carbon dioxide. It 

also contains small amounts of water vapor and particulate matter. Air quality can affect the 

growth of plants in agricultural systems (Darley and Middleton, 1966) as well as human and 

animal health.

Agronomic practices used in agriculture affect air quality. Tillage exposes soil to wind erosion 

and utilizes motorized equipment that produces emissions. The use of herbicide-resistant crops 

has facilitated the adoption of conservation tillage (Towery and Werblow, 2010). Reduced 

tillage generates fewer particulates (dust), so potentially contributes to lower rates of wind 

erosion and release of soil particulates into the air, thus, benefitting air quality (Fawcett and 

Towery, 2002). Conservation tillage also minimizes the use of mechanized equipment that 

produces exhaust, so reduces emissions. Application of agricultural chemicals, such as 

herbicides or insecticides can also affect air quality. 

3.3.4 Climate Change

Climate change represents a significant, sustained statistical change in average weather 

conditions over a broad region. EPA has identified CO2, methane (CH4), and nitrous oxide 

(N2O) as the most important GHGs (greenhouse gases) contributing to climate change. While 

each of these occurs naturally in the atmosphere, human activity has significantly increased their 

concentration since the beginning of the industrial revolution. The level of human-produced 

gases has been accelerating since the end of World War II, when industrial and consumer

consumption expanded greatly. Since the advent of the industrial age, the percent increase in the 

concentration of some important GHGs are as follows: CO2, 36; CH4,, 148 and N2O, 18 (USEPA, 2011a).

Agriculture, including land-use changes for farming, is estimated to be responsible for 8% of all 

human-induced GHG emissions in the U.S. (Massey and Ulmer, 2010). Many agricultural 

activities affect air quality, including smoke from agricultural burning, machinery, and N2O 

emissions from the use of nitrogen fertilizer (Hoeft et al., 2000; Aneja et al., 2009; US-EPA, 

2012a). Emissions released from agricultural equipment (e.g., irrigation pumps and tractors) 

include carbon monoxide, nitrogen oxides, reactive organic gases, particulate matter, and sulfur 

oxides (US-EPA, 2012a). Tillage contributes to GHG production because it releases CO2 

sequestered in soil and promotes oxidation of soil organic matter (Baker et al., 2005).

3.4 BIOLOGICAL RESOURCES

This section provides a summary of the biological environment and includes an overview of 

animals, plants, microorganisms, and biodiversity associated with soybean production. This 
Final BASF CV127 Soybean EA

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summary provides the foundation to assess the potential impact to plant and animal communities, 

the potential for gene movement, and the potential for human health impacts.

3.4.1 Animals Communities

Wildlife may be found within or near soybean fields. Deer and groundhogs feed on soybean and 

cause soybean damage, whereas feeding damage from Eastern cottontail, raccoon, squirrels, and 

other rodents is of less importance (MacGowan et al., 2006). Migratory birds may feed on 

spilled soybean following harvest; contrasted with corn, however, soybeans are harvested close 

to the ground which leaves few alternative food sources or little standing crop residue for 

protective cover (Galle et al., 2009). 

Reptiles and amphibians associated with soybean cultivation primarily inhabit the uncultivated 

lands adjacent to cultivated fields, particularly those lands associated with conservation reserve 

protection borders (See also, Stamps et al., 2008). These areas would include drainage ditches, 

open grassland, and flooded areas (Sharpe, 2010). Fish may inhabit wetlands, ponds, lakes, 

streams, and rivers proximate to soybean cultivation areas (Palmer et al., 2010; Sharpe, 2010). 

Crop pest insects are considered less problematic than weeds in U.S. soybean production; 

nevertheless, insect injury can impact yield, plant maturity, and seed quality. A variety of insect 

pests may be found in U.S. soybean fields, including those that feed on reproductive tissue, 

foliage, and roots/nodules. Insect pests are managed during the growth and development of 

soybean to enhance soybean yield (Higley, 1994; Aref and Pike, 1998). Although insect injury

can impact yield, plant maturity, and seed quality, insect injury in soybean seldom reaches levels 

to cause an economic loss, as indicated by the low percentage (16%) of the 2006 soybean 

acreage that receives an insecticide treatment (USDA-NASS, 2007).

There are numerous beneficial arthropods that are predators or parasites of the arthropod pests of 

soybean, including predatory insects and spiders and parasitoids (Stewart et al., 2007).

Examples include representatives of several arthropod taxa: Hemiptera (the true bugs), such as 

pirate bugs (Orius spp.) and Nabis spp. (damsel bugs); Coleoptera (the beetles) such as, carabid 

beetles (ground beetles) and Coccinellid ladybird beetles; Neuroptera (the lacewings); 

Hymenoptera (parasitic wasps); Diptera (the flies), such as the syrphid or flower flies; and

Arachnids (spiders) such as the Opilones (harvestmen), and Aranae (spiders) (Van Duyn, 2000; 

Grantham and Arnold, 2007; Stewart et al., 2007). 

Honeybees are generally not considered an important part of the beneficial invertebrate 

community in soybeans. Honeybees are not essential to soybean pollination. Soybean is selfpollinated (OECD, 2000). Although low-levels of cross-pollination can occur, this outcrossing 

has been observed with surrounding plants; insect activity has been found to increase the 

outcrossing rate, but soybean is not a preferred plant for pollinators (Jaycox, 1970a; Jaycox; 

Erickson, 1975 1010; Abrams et al., 1978). Additionally, soybean does not produce a lot of 

pollen and so would not be a significant source of protein for honeybees, however soybean flour 

can be used to provide supplemental protein to bee colonies to support low levels of brood 

rearing during winter months in the South, Southwest, and Southeast when pollen sources are 

low (Standifer et al., 1977). 
Final BASF CV127 Soybean EA

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3.4.2 Plant Communities

The vegetative landscape surrounding a soybean field varies with region; soybean fields may be 

surrounded by additional soybean varieties, other crops, or woodland/pasture/grassland areas.

Weeds are perceived to be the most substantial pest problem in soybean production, negatively 

affecting yield through competition for light, nutrients, and moisture (Aref and Pike, 1998).

When weeds are left to compete with soybean for the entire growing season, yield losses can 

exceed 75% (Dalley et al., 2001). 

Annual weeds are perceived to be the greatest pest problem in soybean production, followed by

perennial weeds (Aref and Pike, 1998). In the Southeast, the most common weeds are 

Morningglory spp. (Ipomoea spp.), crabgrass (Digitaria spp.), Prickly sida (Sida spinosa), 

Nutsedge (Cyperus esculentus), Sicklepod (Senna obtusifoliia), Signalgrass (Brachiaria 

platyphylla), and Palmer amaranthus and related pigweeds (Amaranthus palmeri and 

Amaranthus spp.) (Webster et al., 2005; Webster et al., 2009; Heap, 2013).

Soybean is not native to the U.S. and there are no feral or weedy relatives (USDA-APHIS, 

2012). Consequently, soybean in the U.S. can cross only with other soybean varieties.

Additionally, potential of soybean weediness is low, due to domestication syndrome traits that 

generally lower overall fitness outside an agricultural environment (Stewart et al., 2003). Mature 

soybean seeds have no innate dormancy, are sensitive to cold, and are not expected to survive in 

freezing winter conditions (OECD, 2000; Carpenter et al., 2002).

Cultivated soybean is highly self-pollinating (Ahrent and Caviness, 1994). When soybean plants 

are grown directly adjacent to other soybean plants, the amount of natural cross pollination has 

generally been found to be 0.5 - 1% (OECD, 2000; Fehr, 2007) although higher values (up to 

2.5%) have been noted in some varieties (Abud et al., 2003). Outcrossing can be reduced to 0 –

0.01% with a separation distance of 10 meters (Abud et al., 2007; USDA-APHIS, 2012). 

3.4.3 Soil Microorganisms

Various bacterial and fungal species have been identified as the causal agents of various diseases 

afflicting soybean plants (Ruhl, 2007). Additionally, soil microorganisms may play a key role in 

dynamic biochemical soil processes (Garbeva et al., 2004). They may also suppress soil-borne 

plant diseases and promote plant growth (Doran et al., 1996). The main factors affecting 

microbial population size and diversity include soil type, plant type, and agricultural 

management practices (Garbeva et al., 2004). Microbial diversity in the rhizosphere1 may be 

extensive and differ from the microbial community in the bulk soil (Garbeva et al., 2004).

3.4.4 Biological Diversity

Biological diversity, or biodiversity, refers to all plants, animals, and microorganisms interacting 

in an ecosystem (Wilson, 1988). Biodiversity provides valuable genetic resources for crop 

 

1 The rhizosphere is defined as subsoil area in the root zone of plants in which plant roots compete with the invading 

root systems of neighboring plants for space, water, and mineral nutrients, and interact with soil-borne microorganisms, including bacteria, fungi, and insects feeding on the organic material in the soil T. S. Walker, H. P. Bais, E. 

Grotewold and J. M. Vivanco, "Root Exudation and Rhizosphere Biology," Plant Physiology 132.1 (2003)..
Final BASF CV127 Soybean EA

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improvement and also provides other functions beyond food, fiber, fuel, and income (Harlan, 

1975). These include pollination, genetic introgression, biological control, nutrient recycling, 

competition against natural enemies, soil structure, soil and water conservation, disease 

suppression, control of local microclimate, control of local hydrological processes, and 

detoxification of noxious chemicals (Altieri, 1999). The loss of biodiversity results in a need for 

costly management practices in order to provide these functions to the crop (Altieri, 1999). 

The degree of biodiversity in an agroecosystem depends on four primary characteristics: 1) 

diversity of vegetation within and around the agroecosystem, 2) permanence of various crops 

within the system, 3) intensity of management, and 4) extent of isolation of the agroecosystem 

from natural vegetation (Altieri, 1999). 

Agricultural land subject to intensive farming practices, such as that used in crop production, 

generally has low levels of biodiversity compared with adjacent natural areas; tillage, seed bed 

preparation, planting of a monoculture crop, pesticide use, fertilizer use, and harvest limit the 

diversity of plants and animals (Lovett et al., 2003). 
Final BASF CV127 Soybean EA

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4 ENVIRONMENTAL CONSEQUENCES

4.1 SCOPE OF THE ENVIRONMENTAL ANALYSIS AND ASSUMPTIONS

BASF CV127 Soybean was developed for cultivation primarily in Brazil and Argentina. BASF 

does not intend to commercially cultivate BASF CV127 Soybean in the U.S. (BASF, 2011). 

Soybeans are naturally tolerant of imazethapyr, which is currently labeled for use on soybeans 

(Senseman, 2007; BASF, 2011). Imazapyr is not currently registered for use on soybeans in the 

U.S., and no label for use has been submitted to EPA. According to the developer, there is a 

very small market in the U.S. for this soybean event; the projected market would not support the 

cost associated with pursuing EPA registration (BASF, 2011). 

BASF presents the results of field trials comparing BASF CV127 Soybean with conventional 

isolines for agronomic, phenotypic and ecological interactions (See Appendix F in BASF 

petition) (BASF, 2011). In these field trials, BASF conducted field observations on seed 

germination rate, seedling vigor, days to reach select development stages, plant height and grain 

yield, and susceptibility to and interactions with diseases and insects (BASF, 2011). Results of 

these studies demonstrate that BASF CV127 Soybean is not statistically different from other 

conventional soybean varieties (BASF, 2011; USDA-APHIS, 2012).

Under the Preferred Alternative, USDA-APHIS assumes that U.S. growers could plant BASF 

CV127 soybeans, if they were available. Growers could use any management practices that are 

suitable for the production of soy, including EPA registered herbicides. Growers could not use 

imazapyr because it is not labeled for use on soybeans in the U.S.

4.2 SOCIOECONOMICS

4.2.1 No Action - Socioeconomics

4.2.1.1 No Action - Areas and Acreage of Soybean Production

Soybean acreage in the U.S. over the past five years has been relatively stable varying from 75 

million to 77 million acres with a 10-year average of 73.3 million acres (USDA-NASS, 2012a).

Fluctuations in soybean acreage are due to environmental, agronomic, and economic factors, as 

well as government programs such as the conservation reserve program (CRP) or ethanol 

mandates imposed by the U.S. government which drive acreage into source crops for ethanol

(USDA-ERS, 2012c).

GE- and non-GE soybean varieties are continually under development. In 2012, soybean was 

cultivated on over 75 million acres (USDA-NASS, 2012a). Approximately 93% of U.S. soybean

acreage is planted with GE soybean (USDA-ERS, 2012a; USDA-NASS, 2012a). Acreage 

dedicated to soybean production is expected to continue within the 10-year average of 73.3 

million acres.

Government programs and incentives can influence the amount of soybean that is planted in the 

U.S. For example, the establishment of a bioethanol industry using corn as a feed influenced the 

decrease in acreage devoted to soybean, with more than 27% of the corn harvest dedicated to 
Final BASF CV127 Soybean EA

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corn-based biofuel production (NCGA, 2012 705; USDA-ERS, 2012d). Nationwide, soybeans 

are most commonly grown in a crop rotation with corn (USDA-ERS, 2012f). In the Southern 

U.S., rotation crops include corn, soybean, sorghum, cotton, wheat, and rice, with continuous 

soybean the most commonly reported practice (USDA-NASS, 2012j). 

In 2011, 77 million acres of soybeans were planted in the U.S., yielding 3.1 billion bushels (84.4 

MMT) at a value of 35.8 billion U.S. dollars (USDA-NASS, 2012d). The majority of soybeans 

produced in the U.S. are used domestically for animal feed, with lesser amounts and byproducts 

used for oil or fresh consumption (GINA, 2013; USDA-ERS, 2013a). From 1924 when recording 

U.S. soybean production first began, both soybean acreage and yields have steadily increased 

(Egli, 2008). In comparison, total U.S. cropland has remained relatively level since 1945 

(USDA-ERS, 2011). In the 20 years from 1991 to 2011, U.S. soybean acreage expanded 31% 

and yields increased 17.6% (USDA-NASS, 2012f; USDA-NASS, 2012g) while annual value of 

production increased approximately 222% from approximately $11.09 billion to approximately

$35.8 billion (USDA-NASS, 2012f). 

Soybean supply is a function of the amount of acreage planted and soybean yield on those acres. 

While domestic soybean yield has increased, current demand for soybean products has also 

increased, mitigating any downward pressure on farm soybean prices from potentially increased 

supply (NRC, 2010). The national average of U.S. soybean yield is expected to increase 

between 0.4 to 0.5 bushels per acre per year during the same time period, from 44.5 bushels per 

acre projected in 2013/2014 to 48.1 bushels per acre in 2021/2022 at a total increase of 

approximately 8%, without expanding acreage. While overall productivity would increase 

without expanding U.S. soybean acreage, the U.S. farm price per bushel of soybean is predicted 

to vary only from $10.30 to $11.35 from 2013/2014 to 2021/2022. Grower net returns are 

estimated to increase approximately 24% from $303 per acre to $375 per acre over the same 

period, despite an estimated approximately 3% rise in seed and residual costs, and 10.3% rise in 

overall per acre cost of production (USDA-OCE, 2012b). 

In 2007, soybeans were grown on 279,110 farms in the US, with an average of 229 acres in 

soybeans. Farms with fewer than 250 acres accounted for 72% of farms growing soybeans, but 

produced only 26% of the total crop in 2007. Individual or family-owned farms made up 81% of 

all soybean-producing farms and 69% of total soybean production. Partnerships and small 

family-held corporations accounted for 17% of farms with other corporations and institutions 

owning about 1% of soybean producing farms (USDA-NASS, 2009).

USDA estimates annual production costs and returns for major field crops based on grower 

surveys of the actual costs incurred by producers. On average in 2011, estimates of total 

operating costs and allocated overhead for soybean production in the U.S. were $396.70/acre 

with a return over operating costs and overhead of $128.66/acre (USDA-NASS, 2013). The 

2011 cost estimates are projected based on a survey of soybean producers from 2006, the last 

year for which survey data are available, and therefore may not reflect structural or technical 

change that may have taken place since 2006. 

The national averages mask variation in costs and yields. In an analysis of the costs of producing 

U.S. soybeans in 1997, average soybean production costs ranged from $2.13 per bushel to $6.00 

per bushel for the lowest and highest cost quartiles (Foreman and Livezey, 2002). A substantial 
Final BASF CV127 Soybean EA

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yield difference of 20 bushels per acre and $77 in soybean production costs were found between 

high and low cost producers (Foreman and Livezey, 2002). The differences were largely due to 

differences in capital recovery of machinery and equipment, and other costs, with low-cost 

producers realizing half the machinery costs per acre of high-cost producers (Foreman and 

Livezey, 2002). Production practices and growing conditions varied by region, with most of the 

low cost producers residing in the Heartland. Off-farm income was found to be an important 

source of household income for many soybean producers (Foreman and Livezey, 2002).

Total U.S. soybean production was 2.9 billion bushels in 2012, down slightly from 3.1 billion 

bushels in 2011 (USDA-NASS, 2012c). In 2011, the price of soybeans for beans was $11.70 per 

bushel on average, resulting in a total value of production of nearly $36 billion (USDA-NASS, 

2012d). As of November 2012, the U.S. season-average soybean price range was projected at 

$13.90 to $15.90 per bushel (USDA-OCE, 2012a). 

4.2.1.2 No Action - Soybean Seed Production

Soybean seed is produced throughout most of the U.S. soybean-growing regions by companies 

that produce and sell seed, and by toll seed producers, or tollers, which produce certified seed for 

other companies (BASF, 2012 ). Seed production or processing plants clean, condition, and bag 

the harvested soybean seed as well as monitor and inspect all the processes at the plant under 

standards established by the International Organization for Standardization (ISO), and includes 

internal and external audit (ISO, 2009). Field inspections are conducted on seed production fields 

throughout the soybean growing season to evaluate variety purity and ensure soybean plants are 

developing properly (AOSCA, 2009).

Soybean seed production is conducted under standard procedures specified by AOSCA to 

prevent gene flow between varieties (AOSCA, No Date). Several best management practices to 

preserve varietal identity include:

 Maintaining isolation intervals to prevent pollen movement from other soybean sources;

 Planting border rows to capture any pollen present or employing natural pollen barriers; 

and

 Field monitoring for off types, other crops, etc.

Soybean is considered to be highly self-pollinated; therefore, cross-pollination to adjacent 

soybean plants occurs at a very low frequency (Caviness, 1966; OECD, 2000; Ray et al., 2003; 

Abud et al., 2007). Other research has also demonstrated that soybean pollen dispersal is 

restricted to small areas and wind mediated pollination is negligible (Yoshimura, 2011). Several 

factors influence optimal planting rate for soybean such as row spacing, seed germination rate, 

soil conditions, climate, disease and pest pressure, past tillage practices and crop rotation 

(Robinson and Conley, 2007). Seeding rate is also determined by the plant population desired by 

the grower. Growers may plant certified soybean seed, uncertified seed, and “binrun” soybean 

seed that is grown and stored on individual farms (Oplinger and Amberson, 1986). 

Approximately 93% of the soybean acres planted in the U.S. in 2012 were GE varieties (USDAERS, 2012a), about 71.8 million acres were planted with certified seeds, and an estimated 1.3 to 

2.7 million tons of certified soybean planting seeds were required in 2012. 
Final BASF CV127 Soybean EA

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The production of soybean for foundation, registered, certified, or quality control seed require 

biological, technical, and quality control factors required to maintain varietal purity above that 

required for soybean production for grain. The production and certification of soybean seed is 

regulated by state or regional crop improvement agencies and are chartered under the laws of the 

state(s) they serve (e.g., see Mississippi Crop Improvement Association, 2008; Illinois Crop 

Improvement Association, 2013; SSCA, No Date; Virginia Crop Improvement Association, No 

Date). 

Seed genetic purity is maintained to maximize the value of a new variety or cultivar (Sundstrom 

et al., 2002), of which a seed certification process ensures the desired traits remain within purity

standards (Bradford, 2006) (see Subsection 3.1.2, Soybean Seed Production). Seed producers 

routinely submit applications to the AOSCA National Variety Review Boards for review and 

recommendation for inclusion into seed certification programs. For example, in September 2012, 

AOSCA recommended for certification the inclusion of 60 varieties of soybean expressing high 

yield traits by three seed producing companies (AOSCA, 2012).

Comments submitted in previous environmental assessments have expressed concern over the 

availability and supplies of non-GE, open-pollinated, untreated or organic seed. As soybeans are 

largely self-pollinating, concerns over soybean seed stock generally relate to inadvertent 

commingling of seed after harvest. Soybean growers have many standard management practices 

in place to protect and preserve product identity from seed production through harvest and 

market (See e.g., AOSCA, 2009; ASTA, 2011). Numerous providers of organic and non-GE 

seeds are listed by ATTRA and North Carolina State University (ATTRA, 2012; N.C. State 

University, 2012).

Under this alternative, seed production of BASF CV127 in the U.S. would require a permit from 

USDA-APHIS. Growers would need to apply for permits and follow permit conditions if they 

choose to grow this seed for breeding purposes or to export to South American markets for 

planting. 

4.2.1.3 No Action - Organic Soybean Production

The production of organic soybeans represents between 0.13% and 0.22% of U.S. soybean 

production (USDA-NASS, 2012b; USDA-NASS, 2012a). In 2005, 122,217 acres of soybean in 

the U.S. were certified organic, and in 2008, 125,621 acres were similarly certified representing

about 0.20% of total U.S. soybean production (USDA-ERS, 2013b). Certified organic soybeans 

were harvested from 96,080 acres in 2011, the equivalent of 0.13% of the total U.S. soybean 

acreage (USDA-NASS, 2012b; USDA-NASS, 2012a), and was valued at approximately $49.4 

million, capturing roughly 0.17% of the overall soybean crop value for that year (reported at 

$35.8 billion) (USDA-NASS, 2012g; USDA-NASS, 2012h; USDA-ERS, 2013b). Iowa is the 

largest producer of certified organic soybean, followed by Minnesota, Michigan, New York and 

Illinois (USDA-NASS, 2012b). 
Final BASF CV127 Soybean EA

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Table 2: Certified organic soybean acreage by state shows organic soybean acres planted by state 

in 2007, 2008, and 2011. 
Final BASF CV127 Soybean EA

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Table 2: Certified organic soybean acreage by state.

State

Soybean Acres

State

Soybean Acres

2007 2008 2011 2007 2008 2011

Arizona 241 241 NR1 Missouri 7,893 6,441 5,505

Arkansas 8,374 11,172 W2 Nebraska 5,672 8,825 6,211

Colorado 488 3,502 NR New York 1,324 5,038 8,621

Connecticut 9 9 NR North 

Carolina

165 94 W

Delaware 25 25 W North Dakota 3,308 3,773 3,288

Idaho 1 1 W Ohio 3,665 3,951 5,634

Illinois 6,277 7,225 6,633 Oklahoma 80 165 NR

Indiana 888 1,104 945 Oregon 0 141 NR

Iowa 6,989 19,913 12,659 Pennsylvania ,1589 1,753 1,280

Kansas 639 2,141 1311 South Dakota 4,531 4,786 3,962

Maine 144 194 W Texas 2,093 2,141 W

Maryland 416 437 1,090 Vermont 0 337 527

Michigan 11,320 11,251 11,699 Virginia 360 363 150

Minnesota 25,518 21,229 16,150 Wisconsin 8,318 9,369 7,622

Source: (USDA-ERS, 2013b)

Notes:

1 NR – Not Reported

2 W – Data withheld by growers and not published by USDA-NASS.

Organic soybean producers generally harvest lower yields than other producers, and incur higher 

operating and capital costs when compared with conventional soybean producers (Heatherly et 

al., 2005; McBride and Greene, 2008; USB, 2011). These higher costs are offset by the market 

premium which organic soybean markets typically enjoy (McBride and Greene, 2008). Organic 

soybean production is occurring in the presence of conventional soybean production using GE 

and non-GE soybean varieties. Organic production plans prepared pursuant to the NOP include 

practical methods to prevent co-mingling of organic and GE soybean. The implementation of 

management practices to avoid pollen from a biotechnology-derived crop in organic or 

conventional soybean production operations is facilitated by the nature of soybean pollination, as 

soybean is highly self-pollinating. Organic and non-GE soybean producers can and have 

effectively implemented practices (e.g., isolation during the growing season, equipment cleaning 

during harvest, and post-harvest separation of harvested seed) that allow them to reasonably 

avoid biotechnology-derived soybean and maintain organic or Non-GE production status 

(Brookes and Barfoot, 2004). 

Typically, organic growers use more than one method to prevent unwanted material from 

entering their fields including: isolation of the farm; physical barriers or buffer zones between 

organic production and non-organic production; planting border or barrier rows to intercept 
Final BASF CV127 Soybean EA

39

pollen; changing planting schedules to ensure flowering at different times; and formal 

communications between neighboring farms (Kuepper, 2003; Baier, 2008).

4.2.1.4 No Action - Soybean Imports and Exports

The U.S. imports oilseeds and oilseed products, although the majority of these imports are 

mainly rapeseed and rapeseed products (e.g. canola oil) from Canada, olive oil from Western 

Europe, and tropical oils from the Philippines, Indonesia and Malaysia (USDA-ERS, 2012g). In 

2012, for example, the U.S. imported nearly $125 million of vegetable oil from Argentina, and 

$24 million of vegetable oil from Brazil, in the aggregate representing approximately 7% of the 

total imports of vegetable oils from Canada (USDA-FAS, 2013b).

The U.S. has been importing increasing quantities of soybeans over the past decade (Figure 2). 

Canada provides the majority of these soybeans to U.S. market. In 2012, approximately 6% of 

the total soybean imports were from South America (Figure 3).

Figure 2: Soybeans imported to the U.S, 2003-12.

0

100000

200000

300000

400000

500000

600000

Imported Soybeans

metric tons of soybean

value in in thousands

of dollars
Final BASF CV127 Soybean EA

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Figure 3: Countries from which the U.S. imported soybeans in 2012.

Six countries were the origin of more than 99% of the soybeans imported to the U.S. in 2012. 

About 6% of the soybeans were imported from South America. Under the no action alternative, 

importers of BASF CV127 soybeans would need to obtain a permit or acknowledged notification 

from USDA. These soybeans would need to be segregated in the country of origin to ensure that 

they were not introduced in the U.S. inadvertently in commodity shipments. 

The U.S. is the world’s largest producer and exporter of soybeans, accounting for 35% of total 

world production and 41% of global soybean oilseed exports in 2011-12 (USDA-FAS, 2012c; 

USDA-FAS, 2012b). Brazil and Argentina are the next largest soybean producing countries in 

2011-12, accounting for 28% and 17% of total world production respectively. China is the fourth 

largest soybean producer, producing 6% of the world total soybean crop in 2011-12 (USDAFAS, 2012c). The U.S. share of global exports has steadily diminished over the past 25 years, 

largely due to the expansion of soybean production in South America (USDA-ERS, 2012g). The 

USDA projects that by 2012/2013, Brazil soybean exports will surpass those of the U.S., and 

Argentina soybean exports will increase by 50% (USDA-FAS, 2012b).

In 2011-12, U.S. exports of soybeans, soybean cake and meal and soybean oil totaled over $22 

billion (USDA-FAS, 2012a). China is the largest importer of U.S. soybeans and soybean 

products, accounting for 48% of the total value of U.S. soybean and soybean product exports, 

followed by Mexico (8.3% of total) and Japan (5.2%) (USDA-FAS, 2012a). Because BASF 

CV127 would not be grown in the U.S. except under permit it would not affect the U.S. export 

businesses.

BASF has submitted applications to Brazil and Argentina for approval for cultivation, as well as 

for consumption as food or feed. Approval of the Brazil National Technical Committee for 

Biosafety was obtained in December 2009 and from the Secretariat of Agriculture, Livestock and 

Fisheries of the Argentine Republic in March 2013.

74%

16%

4%

4%

1% 1%

Soybean Imports 2012

Canada

China

India

Argentina

Chile

Uruguay
Final BASF CV127 Soybean EA

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Approvals for food and feed uses have also been obtained in countries that import significant 

numbers of soybeans, including:

 Ministry of Health, Labor and Welfare and the Ministry of Agriculture, Forestry and 

Fisheries of Japan (approved for food and feed March 2013);

 Canadian Food Inspection Agency and Health Canada (approved November 2012);

 Ministry of Agriculture of the People’s Republic of China (received safety certificate

June 2013);

 Food Standards Australia New Zealand (approved for food July 2012) (FSANZ, 2012);

 Republic of China (Taiwan) Food and Drug Administration (approved for food April 

2013);

 Rural Development Administration of the Republic of Korea and the Korean Food and 

Drug Administration (approved for food and feed October 2011 and April 2013,

respectively);

 Bureau of Plant Industry, the Philippines (approved for food, feed and processing in 

October 2010);

 Government of Mexico (approved for food, feed and processing in May 2011);

 Columbian Institute of Agriculture and Livestock (approved for import and feed 

September 2011) and the Health and Social protection Ministry of Columbia (approved 

for import and food January 2012);

 Republic of South Africa Department of Agriculture, Forestry and Fisheries (approved 

for food and feed June 2012); and

 Russian Federation’s Federal Service for veterinary and Phytosanitary Surveillance 

(approved for feed September 2012), and Federal Service on Consumer’s Rights 

Protection and Well-being Surveillance (approved for food December 2012) (McKean, 

2013).

These approvals will not be affected if APHIS were to choose the No Action Alternative. 

4.2.2 Preferred Alternative - Socioeconomic Effects

The Preferred Alternative is not expected to change the effects on Socioeconomic or Human 

Health related issues when compared to the No Action Alternative except for the effects on 

imports of soybeans from countries producing BASF CV127 and for breeders or seed producers 

that might grow this seed for overseas markets. In both of these cases the Preferred Alternative 

has less regulatory burden than the No Action Alternative.

4.2.2.1 Preferred Alternative - Areas and Acreage of Soybean Production

BASF presented the results of field trials comparing BASF CV127 Soybean with conventional 

isolines for agronomic, phenotypic and ecological interactions (BASF, 2011, at Appendix F). In 

these field trials, BASF conducted field observations on seed germination rate, seedling vigor, 

days to reach select development stages, plant height and grain yield, and susceptibility to and 

interactions with diseases and insects (BASF, 2011). Results of these studies demonstrate that 

BASF CV127 Soybean is not statistically different from other conventional soybean varieties

(BASF, 2011; USDA-APHIS, 2012). Therefore, if growers were to grow these soybeans in the 

U.S. the production practices would not change when compared to the no action alternative. 

Imazapyr is not labeled for use on soybeans in the U.S. It is not legal to apply the herbicide and 
Final BASF CV127 Soybean EA

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so there is no change in herbicides application associated with this soybean variety if grown in 

the U.S. 

Currently, BASF CV127 Soybean is in Maturity Group VIII. If BASF CV127 Soybeans are 

grown in the U.S. for research and development or off-season seed production, that cultivation 

would be would most likely be in areas of Louisiana, Mississippi, Alabama and Georgia, Florida 

and the coastal areas of South Carolina (Figure 1). Under the preferred alternative, breeders 

could choose to incorporate this trait into other maturity groups through breeding programs. 

However, because its target market is in South America and the herbicides to which this soy is 

resistant are not labeled for use on soybean in the U.S. this breeding is not likely. CV127 

Soybean is resistant imazapyr, which is not labeled for use on soybean in the U.S. Therefore,

there is no incentive to breed this trait into varieties suitable for planting in the U.S. 

There are no changes in agronomic characteristics in BASF CV127 Soybean that would result in 

a change in the area where soybean is cultivated in the U.S. or an increase in acreage relative to 

the No Action Alternative (USDA-APHIS, 2012). The trend in soybean planting acreage is a 

function of market conditions and is not specific to a single GE soybean variety (Wallander et 

al., 2011) the preferred alternative will not change these influences. 

4.2.2.2 Preferred Alternative - Soybean Seed Production

Approval of the petition for nonregulated status for BASF CV127 Soybean under the Preferred 

Alternative would not change the availability of soybean seed. Growers who produce BASF 

CV127 would not require a permit or acknowledged notification to plant the variety for breeding 

or off season seed production for South American markets. Therefore, there is a lower 

regulatory burden on seed growers under the Preferred Alternative.

4.2.2.3 Preferred Alternative - Organic Production

BASF CV127 is not likely to be grown in the U.S. except for breeding or seed production. 

BASF has presented results of agronomic trials that demonstrate that BASF CV127 Soybean is 

not different in plant growth, yield, and reproductive capacity from other commercial soybeans

(BASF, 2011). As described in the No Action Alternative, organic producers employ cultivation 

methods to prevent the comingling of organic and GE soy. The practices currently employed in 

organic production systems would not change when compared to the No Action Alternative.

4.2.2.4 Preferred Alternative - Soybean Imports and Exports

The U.S. is the world’s largest exporter of soybeans, its share of the export market has 

diminished over the past 25 years largely as a result of expansion of soybean production in South 

America (USDA-ERS, 2012g). The USDA projects that in 2013 Brazil is expected to surpass the 

U.S. as the largest soybean exporter (USDA-ERS, 2012g; USDA-FAS, 2012b; USDA-FAS, 

2013a). The cultivation of BASF CV127 Soybean in Brazil or Argentina may contribute to the 

trend by providing growers in these countries with alternative weed management options. 

Imports of BASF CV127 soybeans from these countries would not require permits, so there 

would be no need to segregate these soybeans under the Preferred Alternative. This would lower 

the regulatory burden for imports when compared to the No Action Alternative.
Final BASF CV127 Soybean EA

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4.3 HUMAN HEALTH AND ANIMAL FEED

4.3.1 No Action - Human Health and Animal Feed

4.3.1.1 No Action - Food and Feed

The general population of the U.S. is most likely to consume soybean products or consume foods 

containing or prepared with soybean oil. Soybeans yield both solid (meal) and liquid (oil) 

products. Soybean meal is high in protein and is used for products such as tofu, soymilk, meat 

replacements, and protein powder; it also provides a natural source of dietary fiber (USB, 2009). 

Nearly 98% of soybean meal produced in the U.S. is used as animal feed, while less than 2% is 

used to produce soy flour and proteins for food use (Soyatech, 2011). Extracted soybean liquid

oils are used to produce salad and cooking oils, baking and frying fat, and margarine. Soy oil is 

low in saturated fats, high in poly and monounsaturated fats, and contains essential omega-3 fatty 

acids. Soybean oil comprises nearly 70% of the oils consumed in U.S. households (ASA, 2010).

Animal agriculture consumes 98% of the U.S. soybean meal produced (Soyatech, 2011) and 70% 

of soybeans worldwide (USB, 2011). Poultry consume more than 48% of domestic soybean 

meal or 11.92 million MT of the U.S. soybean crop, with soy oil increasingly replacing animal 

fats and oils in broiler diets (USB, 2011; ASA, 2012). Soybean can be the dominant component 

of livestock diets, such as in poultry, where upwards of 66% of their protein intake is derived 

from soy (Waldroup and Smith, No Date). Other animals fed domestic soybean by crop volumes 

consumed include swine (26%), beef cattle (12%), dairy cattle (9%), other (e.g., farm-raised fish 

3%), and household pets (3%) (ASA, 2010; USB, 2011).

Although the soybean market is dominated by seed production, soybean has a long history and a 

standing in the U.S. as a nutritious grazing forage, hay, and silage crop for livestock (Blount et 

al., 2009). Soybean may be harvested for hay or grazed from the flowering stage to near 

maturity; the best soybean for forage is in the beginning pod stage (Johnson et al., 2007). For 

silage, it should be harvested at maturity before leaf loss, and mixed with a carbohydrate source,

such as corn, for optimal fermentation characteristics (Blount et al., 2009). Varieties of soybean 

have been developed specifically for grazing and hay, but use of the standard grain varieties are 

recommended by some because of the whole plant feeding value (Weiderholt and Albrecht, 

2003). 

Growers must adhere to EPA label use restrictions for pesticides used to produce a soybean 

crop before using it as forage, hay, or silage. Pursuant to FFDCA, before a pesticide can be 

used on a food crop EPA must establish the tolerance value, which is the maximum amount of 

pesticide residue that can remain on the crop or in foods processed from that crop (US-EPA, 

2010a). In addition, the FDA and the USDA monitor foods for pesticide residues and enforce 

these tolerances (USDA-AMS, 2011). If pesticide residues are found to exceed the tolerance 

value, the food is considered adulterated and may be seized. The USDA has implemented the 

Pesticide Data Program (PDP) in order to collect data on pesticides residues on food (USDAAMS, 2012). The EPA uses PDP data to prepare pesticide dietary exposure assessments 

pursuant to the 1996 Food Quality Protection Act (FQPA). Tolerance levels for various 

pesticides have been established for a wide variety of commodities, including soybean, and are 

published in the Federal Register, CFR, and the Indexes to Part 180 Tolerance Information for 
Final BASF CV127 Soybean EA

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Pesticide Chemicals in Food and Feed Commodities (US-EPA, 2011b).

The EPA Registration Eligibility Decision (RED) for imazapyr, for example, found no human 

health risks from dietary uptake (US-EPA, 2006b). As the imidazolinone herbicides are 

currently used on many different crops, there are currently over 20 different residue tolerances 

for this herbicide on different crops (40 CFR §180.500). To facilitate foreign production and 

possible importation, BASF has applied to the EPA for an import residue tolerance for the use of 

imazapyr and imazapic on soybean (BASF, 2011). 

4.3.1.2 No Action - Worker Safety

The availability of GE, non-GE and organic soybeans would not change as a result of the 

continued regulation of BASF CV127 soybean. Agronomic practices used for soybean 

production include the application of agricultural chemicals (pesticides and fertilizers). Growers 

choose agronomic practices based on weed, insect and disease pressures, cost of seed and other 

inputs, technology fees, human safety, potential for crop injury, and ease and flexibility of the 

production system (Heiniger, 2000; Farnham, 2001; University of Arkansas, 2006). Worker 

safety is taken into consideration by EPA in the pesticide registration process and reregistration 

process. Pesticides are regularly reevaluated by EPA for each pesticide to maintain its registered 

status under FIFRA. Furthermore, the OSHA requires all employers to protect their employees 

from hazards associated with pesticides and herbicides. When used according to label directions, 

pesticides can be used with a reasonable certainty of no harm to human health and without 

posing unreasonable risks to the environment.

4.3.2 Preferred Alternative - Human Health and Animal Feed

4.3.2.1 Preferred Alternative - Food and Feed

Under the Preferred Alternative the effects on food and feed are no different than under the No 

Action Alternative. BASF CV127 is compositionally similar to other commercially available 

soybeans. BASF has presented data comparing BASF CV127 Soybean with other varieties 

(BASF, 2011). No biologically significant differences were identified between BASF CV127 

Soybean and other varieties. BASF has evaluated the potential toxicity of the AtAHAS protein 

expressed by the BASF CV127 Soybean and has found no evidence of acute or chronic toxicity, 

allergenicity, or other health impacts (BASF, 2011; BASF). FDA completed its consultation on 

this product on February 17, 2012 (US-FDA, 2012). 

4.3.2.2 Preferred Alternative - Worker Safety

A determination of nonregulated status of BASF CV127 soybean is not expected to result in 

changes in current soybean cropping practices. Similar to the No Action Alternative, it is 

expected that EPA-registered pesticides, fertilizers, and other chemicals that currently are used 

for soybean production would continue to be used by growers. EPA’s core pesticide risk 

assessment and regulatory processes ensure that each registered pesticide continues to meet the 

highest standards of safety including all populations of non-target species and humans, and if 

used in accordance with the label, can be demonstrated to pose “a reasonable certainty of no 

harm to humans” and “no unreasonable adverse effects to the environment.” Growers are 
Final BASF CV127 Soybean EA

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required to use pesticides consistently with instructions for application provided on the EPAapproved pesticide label. These label restrictions carry the weight of law and are enforced by 

EPA and the states (Federal Insecticide, Fungicide, and Rodenticide Act 7 USC 136j (a)(2)(G) 

Unlawful Acts). Overall impacts to occupational health and safety under the Preferred 

Alternative are expected to be similar to the No Action Alternative.

4.4 PHYSICAL ENVIRONMENT

4.4.1 No Action Alternative – Physical Environment

4.4.1.1 No Action Alternative - Soil Quality

Cultivation and tillage practices can directly impact the attributes of soil, including its physical 

and biological properties. Although practices such as tillage, fertilization, the use of pesticides 

and other management tools can improve soil health, they can also cause substantial damage if 

not properly used. Several concerns relating to agricultural practices include increased erosion, 

soil compaction, degradation of soil structure, nutrient loss, increased salinity, change in pH, and 

reduced biological activity (USDA-NRCS, 2006c).

The practice of tillage for soil preparation and weed management can affect the quality of soils 

because of the varying impacts of erosion on soil nutrient composition. Field preparation is 

accomplished through a variety of tillage systems, with each system defined by the remaining 

plant residue on the field (US-EPA, 2012a). Conventional tillage is associated with intensive 

plowing and less than 15% crop residue; reduced tillage is associated with 15 to 30% crop 

residue; and conservation tillage, including no-till practices, is associated with at least 30% crop 

residue and substantially less soil erosion than other tillage practices (US-EPA, 2012a). As 

conservation practices are adopted, there are increases in soil organic matter that helps bind soil 

nutrients and corresponding significant reductions over time in the loss of cropland soil from 

runoff, erosion, and leaching (Leep et al., 2003; USDA-NRCS, 2006c; USDA-NRCS, 2006b).

Total soil loss on highly erodible croplands and non-highly erodible croplands decreased from 

462 million tons per year to 281 million tons per year or by 39.2% from 1982 to 2003 (USDANRCS, 2006b). This decrease in soil erosion carries a corresponding decrease in non-point 

source surface water pollution of fertilizer and pesticides (USDA-NRCS, 2006b). The reduction 

in soil erosion is also attributed to a decrease in the number of acres of highly erodible cropland 

being cultivated (USDA-NRCS, 2006b).

Other methods to improve soil quality include careful management of fertilizers and pesticides; 

use of cover crops to increase plant diversity and limit the time soil is exposed to wind and rain; 

and, increased landscape diversity with buffer strips, contour strips, wind breaks, crop rotations, 

and varying tillage practices (USDA-NRCS, 2006c). The USDA requires that growers adopt 

these same conservation measures to protect soil on highly erodible lands in order to qualify for 

crop insurance and associated Federal loans (USDA-ERS, 2012c). 

There are a multitude of organisms associated with soils, ranging from microorganisms to larger

organisms, such as worms and insects. One of the microorganisms associated with soybean 

directly affects soil conditions, and bears introduction in this subsection. A legume, the soybean 

plant fixes a significant portion of its own nitrogen through the symbiotic relationship with the 

nitrogen-fixing Bradyrhizobia bacteria (B. japonicum) that live in soybean root nodules (Hoeft et 
Final BASF CV127 Soybean EA

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al., 2000). The soybean root nodules contain colonies of bacteria which take gaseous nitrogen 

from the atmosphere and fix it into a form that is more biologically available to the soybean 

plant. Because the nitrogen-fixing bacteria are not native to U.S. soils and would not normally be 

found in these soils, soybeans are frequently inoculated with these bacteria prior to planting, 

especially if soybean has not been grown in a field for three to five years (Pedersen, 2007). The 

relationship between these nodule bacteria and soybean cultivation is discussed in Subsection 

4.5.1 – Biological Resources: No Action Alternative - Soil Microorganisms. 

Growers applying imidazolinone herbicides for the control of weeds must be cognizant of the 

potential impacts of herbicide carryover (soil residuals) on rotational crops (Hahn and 

Stachowski, 2003; Heiser, 2007; Senseman, 2007; Hager and Refsell, 2008). Imidazolinone 

herbicides are already used on soybeans, and Lightning®is used on the Clearfield®varieties. In 

2007, Imidazolinone herbicides were used on less than 4% of U.S. soybean acreage (USDANASS, 2007a). 

Imidazolinone herbicides are mobile in soil, and have a variable residual half-life depending on 

the formulation and soil conditions (US-EPA, 1989). For example, the half-life of imazamox has 

been reported at 1.4 weeks, imazethapyr has been reported at 16 weeks, and for imazaquin has 

been reported at 191 weeks (Aichele and Penner, 2005). Imazapyr has been determined to be 

persistent and mobile in soil, with half-lives measured in the laboratory ranging from 1.2 years to 

5.9 years. (US-EPA, 2005a). Because of the known soil residual activity associated with several 

of the imidazolinone herbicides (notably imazapic which may exhibit residual weed control 

activity for up to two years, but also imazequin, imazamethabenz, and imazethapyr), (see e.g., 

Heiser, 2007; Senseman, 2007; Hager and Refsell, 2008), growers must be cognizant of the 

sensitivity of crops in their rotation scheme to these herbicides (Heiser, 2007; Senseman, 2007; 

Hager and Refsell, 2008). For example, although imazaquin is already applied to soybean, 

several rotational crops, including barley, corn, cotton oats and sugar beets are acknowledged to 

be susceptible to residues in soil (Senseman, 2007). The persistence of imidazolinone herbicides 

in soil is a function of soil microbial populations, moisture, organic matter, soil pH, soil 

composition and temperature (Heiser, 2007). 

4.4.1.2 No Action Alternative - Water Resources

Soil texture and structure are key components determining water availability in soils which, in 

turn, affects soybean root depth and density (Helsel and Helsel, 1993). Specific soil types have 

varying abilities to hold water; sandy soils retain the least amount of water, and silt loam and

clay loam soils hold the most (Helsel and Helsel, 1993). However, although clay soils hold more 

water, less water is available to the plant because water adheres strongly to the clay particles 

(Helsel and Helsel, 1993). Soybean root depth and distribution are influenced by available water 

and soil physical characteristics, with deepest root systems identified in sand and clay soils, and 

shallowest in loam soils (Dwyer et al., 1988). 

Soil erosion and runoff contribute nonpoint source pollution (NPS) to surface water. Rainfall or 

snowmelt runoff moving over the ground picks up and carries away soil and materials associated 

with the soil, such as fertilizer and pesticides, creating NPS pollution (see e.g., US-EPA, 1996; 

USDA-NRCS, 2006b). Agricultural non-point sources may derive from many different 

agronomic inputs, including fertilizer and pesticide application, spilled oil and gasoline from 
Final BASF CV127 Soybean EA

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farm equipment, and animal manure (US-EPA, 1996). Agricultural NPS pollution is the leading 

source of impacts to surveyed rivers and lakes and the third largest source of impairment to 

estuaries, as well as a major source of impairment to groundwater and wetlands (US-EPA, 1996). 

In 2009, US-EPA identified sedimentation and turbidity associated with tillage practices as two 

of the top 10 causes of impairment to surface water in the U.S. (US-EPA, 2009a). US-EPA has 

projected conservation tillage to be "the major soil protection method and candidate best 

management practice for improving surface water quality" (US-EPA, 2002). US-EPA identifies 

conservation tillage as the first of its CORE4 agricultural management practices for water quality

protection (US-EPA, 2008a).

Based on the states’ water quality reports, which the US-EPA makes available through its 

National Assessment Database, pesticides in general and herbicides in particular are a relatively 

minor contributor to impairment of surface water in the U.S., compared to 

sedimentation/siltation and turbidity (US-EPA, 2013). Pesticides accounted for less than 1% of 

reported causes of surface water impairment in all but four of the 17 leading U.S. soybeanproducing states (US-EPA, 2013) . In those four states, pesticides accounted for 2-8% of 

reported causes of impairment. Of the pesticides that were reported as contributing to impairment 

among the 17 leading soybean-producing states, almost all are highly persistent chemicals that 

are no longer registered for use in the U.S. (US-EPA, 2013). Imidazolinone herbicides are not 

included on this list.

The soils and climate in the Eastern, Midwestern, and portions of the Great Plains region of the 

U.S. provide sufficient water supplies under normal climatic conditions to produce a soybean 

crop. The general water requirement for a high-yielding soybean crop is approximately 20 to 24

inches of water during the growing season to produce a relatively high yield of 40-50 bushels per 

acre (KSU, 1997; Hoeft et al., 2000). In 2006, when approximately 8% of the total soybean crop 

was irrigated, over 92% of the irrigation supply was from groundwater supply (USDA-ERS, 

2012e). In 2006, irrigated soybean produced an average of 51 bushels per acre, where the 

national average for that year was 42.9 bushels per acre (USDA-NASS, 2012h).

In 2011, 65% of U.S. soybean acres reported some form of conservation tillage (USB, 2011). 

Intensive monitoring of surface water and groundwater proximate to agricultural fields has 

demonstrated that conservation tillage practices can reduce runoff from agricultural lands, 

decreasing NPS pollution of suspended sediment, nutrients from fertilizers, and pesticides (UT, 

2011). Nutrient management reduces agricultural chemical movement into ground or surface 

waters (US-EPA, 2005b).

The EPA considers water resources, and potential contamination of water resources, when 

registering a pesticide under FIFRA. Precautions to protect water resources, including aquatic 

animals and plants, if required, are provided on the pesticide label. Under the No Action 

Alternative, current land acreage and agronomic practices, including irrigation, tillage, and 

nutrient management associated with U.S. soybean production, would not be expected to change. 

These practices and inputs would include the use of imidazolinone herbicides. No expected 

changes to water use beyond current trends associated with soybean production are expected for 

this alternative. 
Final BASF CV127 Soybean EA

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The EPA has considered the potential impacts to water resources from the agricultural 

application of imidazolinone herbicides, and has included label use restrictions and handling 

guidance intended to prevent impacts to water (see e.g., BASF, 2008). Label restrictions for 

Lightning®specific to water resources include prohibiting applications directly to water, 

managing proper disposal of equipment wash water, and adopting cultivation methods to limit 

runoff to surface water (BASF, 2008). The half-life of imazapyr in water is estimated at 3 to 5 

days, and imazapyr is registered for use in aquatic areas and treated water from these sites may

be diverted to irrigate food or feed crops (US-EPA, 2006a). 

4.4.1.3 No Action Alternative - Air Quality

Agricultural activities have the potential to impact air quality. Agricultural air emission sources 

include: smoke from agricultural burning; vehicle exhaust associated with equipment used in 

tillage and harvest; soil particulates associated with tillage; and nitrous oxide emissions from the 

use of nitrogen fertilizer (Hoeft et al., 2000; USDA-NRCS, 2006a; Aneja et al., 2009; US-EPA, 

2012a). Aerial application of pesticides may cause air quality impacts from drift and diffusion, 

and pesticides may volatilize after application to soil or plant surfaces and may also move as 

constituents of entrained materials in wind eroded soils (Vogel et al., 2008). 

The majority of soybean grown in the U.S. is rotated with corn on a two-year rotation (OECD, 

2000; USDA-ERS, 2005). Soybean fields typically are tilled and the new crop rotation planted 

in the following year (Hoeft et al., 2000). Reduced tillage generates fewer particulates (dust) 

entrained by wind erosion (Towery and Werblow, 2010). An additional benefit to air quality

relates to the reduction in emission-producing equipment associated with crop tillage (USDANRCS, 2012).

Open combustion associated with prescribed burning as an agricultural resource management 

strategy produces particles of widely ranging size, depending to some extent on the rate of 

energy release of the fire (US-EPA, 2012a). The extent to which agricultural and other 

prescribed burning may occur is regulated by state air quality requirements to achieve 

compliance with the national ambient air quality standards (US-EPA, 2012a). Prescribed 

burning of fields would likely occur only as a pre-planting option for soybean production based 

on individual farm characteristics.

Volatilization of fertilizers, herbicides, and pesticides from soil and plant surfaces also 

introduces these chemicals to the air. The USDA Agricultural Research Service (ARS) has 

recently reported several long-term studies to identify factors that affect pesticide levels in the 

Chesapeake Bay region airshed (USDA-ARS, 2011). In one study, USDA-ARS has determined 

that pesticide volatilization is highly dependent upon exposure of disturbed unconsolidated soils 

and variability in measured compound levels is correlated with temperature and wind conditions. 

In another ARS study, moisture in dew and soils in higher temperature regimes was found to 

substantially increase volatilization rates (USDA-ARS, 2011). 

Pesticide spraying may impact air quality from drift and diffusion. Drift is defined by EPA as 

“the movement of pesticide through air at the time of application or soon thereafter, to any site 

other than that intended for application” (US-EPA, 2000). Diffusion is gaseous transformation 

to the atmosphere (FOCUS, 2008). Factors affecting pesticide drift and diffusion include 
Final BASF CV127 Soybean EA

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application equipment and method, weather conditions, topography, and the type of crop being 

sprayed (US-EPA, 2000). EPA’s Office of Pesticide Programs (OPP) encourages pesticide 

applicators to use all feasible means available to them to minimize off-target drift, and most 

pesticides where drift is an acknowledged risk include label use restrictions intended to minimize 

off-target drift (see e.g.Micro Flo Company, 2012). The Agency has introduced several 

initiatives to help address and prevent the problems associated with drift. Currently, EPA is 

evaluating new regulations for pesticide drift labeling and the identification of best management 

practices to control such drift, as well as identifying scientific issues surrounding field volatility

of conventional pesticides (US-EPA, 2009b). Additionally, EPA OPP and its Office of Research 

and Development are developing a new voluntary program, the Drift Reduction Technology

(DRT) Program, which encourages the development, marketing and use of application 

technologies verified to substantially reduce spray drift (US-EPA, 2009b).

Traditional agricultural practices have the potential to cause negative impacts to air quality. 

Agricultural emission sources include smoke from agricultural burning, tillage, heavy equipment 

emissions, pesticide drift from spraying, indirect emissions of carbon dioxide and nitrous oxide,

the degradation of organic materials in the soil, and from the use of nitrogen fertilizer (USDANRCS, 2006a; Aneja et al., 2009). 

4.4.1.4 No Action Alternative - Climate Change 

Soil nitrogen sequestration and emissions are affected by multiple field conditions, including soil 

temperature, soil moisture, fertilization, production of nitrogen fixing crops (e.g. soybeans and 

other legumes), retention of crop residues in the soil and decomposition of soil organic matter 

and plant litter (US-EPA, 2012a).

Agriculture, including land-use changes associated with farming, is responsible for an estimated 

6% of all human-induced GHG emissions in the U.S. (US-EPA, 2012a). Agriculture-related 

GHG emissions include CO2, N2O, and CH4, produced through the combustion of fossil fuels to 

run farm equipment; the use of fertilizers; or the decomposition of agricultural waste products, 

including crop residues, animal wastes, and enteric emissions from livestock. N2O emissions 

from agricultural soil management (primarily nitrogen-based fertilizer use) represent 68% of all 

U.S. N2O emissions (US-EPA, 2012a). 

4.4.2 Preferred Alternative – Physical Environment

Because the regulatory decision on the petition for nonregulated status of BASF CV127 Soybean

will not change the availability of soybean varieties or the agronomic practices associated with 

the available soybean varieties, there will be no change to soil quality, water quality or resources, 

air quality, or factors influencing climate change under the preferred alternative when compared 

to the no action alternative. Herbicide use in the U.S. is limited to those applications described 

on the label. This soybean is engineered to be resistant to an herbicide that is not labeled for use 

on soybeans in the U.S. and no application is pending or planned to change that situation. 

Therefore, if these soybeans were acquired by a U.S. grower, they would be cultivated in the 

same way as currently available varieties. Because the effects on these resources result from the 

agronomic practices and these are same the under the No Action and the Preferred Alternative, 

there is no change to soil quality, water resources, air quality, or factors influencing climate 
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change associated with this decision.

4.5 BIOLOGICAL RESOURCES

4.5.1 No Action Alternative – Biological Resources

4.5.1.1 No Action Alternative - Animal Communities

Animal communities in this discussion include wildlife species and their habitats. Wildlife refers 

to both native and introduced species of mammals, birds, amphibians, reptiles, invertebrates, and 

fish/shellfish. Agriculture dominates human uses of land (Robertson and Swinton, 2005). In 

2011, 917 million acres (approximately 47%) of the contiguous 48 states were devoted to 

farming, including: crop production, pasture, rangeland, Conservation Reserve Program, 

Wetlands Reserve Program, or other government program uses (Senseman, 2007; USDA-NASS, 

2012e). How these lands are maintained influences the function and integrity of ecosystems and 

the wildlife populations that they support.

A wide array of wildlife species occur within the 31 major soybean-producing U.S. states. 

During the spring and summer months, soybean fields provide browse for rabbits, deer, rodents, 

other mammals; birds such as upland gamebirds, while also providing a forage base for insects 

(Palmer et al., No Date). During the winter months, leftover and unharvested soybeans provide a 

food-source for wildlife; however, soybeans are poorly suited for meeting nutrient needs of 

wildlife, such as waterfowl, that require a high-energy diet (Krapu et al., 2004). 

Shifts from conventional agricultural practices to conservation tillage and no-till practices has 

benefitted wildlife through improved water quality, availability of waste grain, retention of cover 

in fields, and increased populations of invertebrates (Brady, 2007; Sharpe, 2010). Conservation 

tillage practices that leave greater amounts of crop residue serve to increase the diversity and 

density of birds and mammals (USDA-NRCS, 1999). Increased residue also provides habitat for 

insects and other arthropods, consequently increasing this food source for insect predators. 

Insects are important during the spring and summer brood rearing season for many upland game 

birds and other birds, as they provide a protein-rich diet to fast growing young, as well as a 

nutrient-rich diet for migratory birds (USDA-NRCS, 2003).

Insects and other invertebrates can be beneficial to soybean production, providing services such 

as nutrient cycling and preying on plant pests. Conversely, there are many insects and 

invertebrates that are detrimental to soybean crops, including: bean leaf beetle (Cerotoma 

trifurcata); beet armyworm (Spodoptera exigua); blister beetle (Epicauta spp.); corn earworm 

(Helicoverpa zea); grasshopper (Acrididae spp.); green cloverworm (Hypena scabra); seed corn 

beetle (Stenolophus lecontei); seedcorn maggot (Delia platura); soybean aphid (Aphis glycines); 

soybean looper (Pseudoplusia includens); soybean stem borer (Dectes texanus); spider mites 

(Tetranychus urticae); stink bug (green [Acrosternum hiliare]; brown [Euschistus spp.]); and 

velvetbean caterpillar (Anticarsia gemmatalis) (Whitworth et al., 2011; Palmer et al., No Date). 

While insects are considered less problematic than weeds in U.S. soybean production, insect 

injury can impact yield, plant maturity, and seed quality. Consequently, insect pests are 

managed during the growth and development of soybean to enhance soybean yield (Higley, 

1994; Aref and Pike, 1998). 
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Under FIFRA, all pesticides, (which is inclusive of herbicides) sold or distributed in the U.S.

must be registered by the EPA (US-EPA, 2005b). Registration decisions are based on scientific 

studies that assess the chemical’s potential toxicity and environmental impact. To be registered, 

a pesticide must be able to be used without posing unreasonable risks to the environment, 

including wildlife. All pesticides registered prior to November 1, 1984 must also be reregistered 

to ensure that they meet the current, more stringent standards. During the registration decision, 

the EPA must find that a pesticide does not cause unreasonable adverse effects to the 

environment if used in accordance with the approved label instructions (OSTP, 2001). 

Additionally, growers must adhere to EPA label use restrictions for herbicides and pesticides. 

These measures help to minimize potential impacts of their use on non-target wildlife species. 

EPA is currently evaluating new regulations for pesticide drift labeling and the identification of 

best management practices to control such drift (US-EPA, 2009b), as well as identifying 

scientific issues surrounding field volatility of conventional pesticides (US-EPA, 2010b).

4.5.1.2 No Action Alternative - Plant Communities

Soybeans are grown in 31 states (USDA-NASS, 2012a), encompassing a wide range of 

physiographic regions, ecosystems, and climatic zones. The types of vegetation, including the 

variety of weeds, within and adjacent to soybean fields can vary greatly, depending on the 

geographic area in which the field occurs. Non-crop vegetation in soybean fields is limited by

the extensive cultivation and weed control programs practiced by soybean producers. Plant 

communities bordering soybean fields can range from forests and woodlands to grasslands, 

aquatic habitats, or residential areas. Adjacent crops frequently include other soybean varieties, 

corn, cotton, or other crops.

Weeds are classified as annuals or perennials. An annual is a plant that completes its lifecycle in 

one year or less and reproduces only by seed. Perennials are plants that live for more than two

years. Weeds are also classified as broadleaf (dicots) or grass (monocots). Weeds can reproduce 

by seeds, rhizomes (underground creeping stems), or other underground parts. Annual grass and 

broadleaf weeds are considered the most common weed problems in soybeans (Krausz et al., 

2001); however, with increased rates of conservation tillage, increases in perennial, biennial, and 

winter annual weed species are being observed (Durgan and Gunsolus, 2003) (Green and Martin, 

1996). Some troublesome weeds of soybean include common lambsquarter (Chenopodium 

album), morning glory species (Ipomoea spp.), velvetleaf (Abutilon theophrasti), pigweed, 

(Amaranthus spp.), common cocklebur (Xanthium strumarium), foxtail (Setaria spp.), ragweed 

species (Ambrosia spp.), crabgrass (Digitaria spp.), barynyard grass (Echinochloa crus-galli), 

Johnsongrass (Sorghum halepense), and thistle (Cirsium spp.). Recent surveys of U.S. 

agronomic crop producers suggest that pigweed species, Johnsongrass, foxtail species, and 

velvetleaf are among the most problematic weeds (Heatherly et al., 2009).

An important concept in weed control is the seed bank, which is the reservoir of seeds that are in 

the soil and have the potential to germinate. Agricultural soils contain reservoirs of weed seeds 

ranging from 4,100 to 137,700 seeds per square meter of soil (May and Wilson, 2006). Climate, 

soil characteristics, cultivation, crop selection, and weed management practices affect the seed 

bank composition and size (May and Wilson, 2006).

Herbicide resistance is described by the Weed Science Society of America as the “inherited 
Final BASF CV127 Soybean EA

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ability of a plant to survive and reproduce following exposure to a dose of herbicide normally

lethal to the wild type” (WSSA, 2011). The first reports of weed resistance to herbicides were in 

the 1950s (WSSA, 2011). Individual plants within a species can exhibit different responses to 

the same herbicide rate. Initially, herbicide rates are set to work effectively on the majority of the 

weed population under normal growing conditions. Genetic variability, including herbicide 

resistance, is exhibited naturally in normal weed populations, although at very low frequencies. 

When only one herbicide is used year after year as the primary means of weed control, the 

number of weeds resistant to that herbicide compared to those susceptible to the herbicide may

change as the surviving resistant weeds reproduce. With no change in weed control strategies, in 

time, the weed population may be composed of more and more resistant weeds (WSSA, 2011).

The evolution of herbicide-resistant weeds has required that growers diversify weed management 

practices and use combinations of herbicides, tillage practices, and herbicide-resistant traits. 

Integrated weed management programs that use herbicides from different groups, vary cropping 

systems, rotate crops, and that use mechanical as well as chemical weed control methods, will 

delay or prevent the selection of herbicide-resistant weed populations (Gunsolus, 2002; Sellers et 

al., 2011).

Runoff, spray drift, and volatilization of herbicides have the potential to impact non-target plant 

communities growing in proximity to fields in which herbicides are used. The extent of damage 

to nontarget plants exposed to herbicides is determined by the overall vigor of the affected plant, 

the amount and type of herbicide to which the plant is exposed, and the growing conditions after 

contact (Ruhl et al., 2008).

The total rainfall the first few days after herbicide application can influence the amounts of 

leaching and runoff; however, it has been estimated that even after heavy rains, herbicide losses 

to runoff generally do not exceed 5- to 10% of the total applied (Tu et al., 2001; USDA-FS, 

2009). Planted vegetation, such as grass buffer strips, or crop residues can effectively reduce

runoff (IPPC, 2010). Volatilization typically occurs during application, but herbicides deposited 

on plants or soil can also volatilize. Most of the herbicides considered highly volatile are no 

longer used (Tu et al., 2001).

Spray drift is a concern for non-target susceptible plants growing adjacent to fields when 

herbicides are used in the production of soybeans. This potential impact relates to exposure of 

non-target susceptible plants to the off-target herbicide drift (US-EPA, 2010b). Damage from 

spray drift typically occurs at field edges or at shelterbelts (i.e., windbreaks), but highly volatile 

herbicides may drift further from a field. The risk of off-target herbicide drift is recognized by

the EPA, which has incorporated both equipment and management restrictions to address drift in 

the EPA-approved herbicide labels. These EPA label restrictions include requirements that the 

grower manage droplet size, spray boom height above the crop canopy, restricted applications 

under certain wind speeds and environmental conditions, and using drift control agents (USEPA, 2010b).

Volunteer soybean is not a widespread problem, and when they occur, it is most often in parts of 

the Delta and the southeastern U.S. In production systems where soybean is rotated, such as 

corn or cotton, it has shown up as a volunteer weed, yet was not generally seen as a serious 

problem by farmers (Owen and Zelaya, 2005). Volunteer soybean is not considered difficult to 
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manage, as soybean seeds rarely remain viable the following season and any interference they

may pose to subsequent crops are minimal; furthermore, herbicides usually used for weed control 

in corn are also effective at controlling volunteer soybean (Owen and Zelaya, 2005).

The potential for cross-pollination between soybean varieties is limited. Soybeans are highly

self-pollinating (Ahrent and Caviness, 1994), and the frequency of natural cross pollination 

between varieties grown near to each other has been found to range from 0.0% to 0.5%, when 

row distances ranged from 1m to 10 m (OECD, 2000; Abud et al., 2003; Abud et al.).

4.5.1.3 No Action Alternative - Soil Microorganisms

Soil microorganisms play a key role in soil structure formation, decomposition of organic matter, 

toxin removal, nutrient cycling, and most biochemical soil processes (Garbeva et al., 2004). 

They also suppress soil-borne plant diseases and promote plant growth (Doran et al., 1996). 

Estimates of the number of bacterial species that may be found in a gram of soil range from 

6,000 to 50,000 (Curtis et al., 2002). In a study of soil suppressive to Rhizoctonia solani, a 

fungal pathogen in crops such as potatoes, sugar beets, and rice, (Mendes et al. (2011) found that 

over 33,000 prokaryotic1species were present in the rhizosphere. The soil microbial community

include nitrogen-fixing microbes such as the soybean mutualist B. japonicum, mycorrhizal fungi, 

and free-living bacteria2; bacteria, actinomycetes (filamentous bacteria), and saprophytic fungi 

responsible for decomposition; denitrifying bacteria and fungi; phosphorus-solubilizing bacteria 

and fungi; as well as pathogenic and parasitic microbes (USDA-NRCS, 2004).

The main factors affecting microbial population size and diversity include soil type (texture, 

structure, organic matter, aggregate stability, pH, and nutrient content), plant type (providers of 

specific carbon and energy sources into the soil), agricultural management practices (crop 

rotation, tillage, herbicide and fertilizer application, and irrigation) and cropping history

(Garbeva et al., 2004; Garbeva et al., 2008). Some types of soil micro-organisms share 

metabolic pathways with plants, and might be affected by herbicides. Tillage disrupts 

multicellular relationships among microorganisms, and crop rotation changes soil conditions in 

ways that favor different microbial communities.

Plant roots, including those of soybean, release a variety of compounds into the soil creating a 

unique environment for microorganisms in the rhizosphere (root zone). Microbial diversity in 

the rhizosphere may be extensive and differs from the microbial community in the bulk soil 

(Garbeva et al., 2004). The following briefly focuses on the soybean, GE crops, and herbicide 

use factors with the potential to affect microbial population size and diversity. 

Soybeans

An important group of soil microorganisms associated with legumes, including soybean, are the 

mutualists. These include mycorrhizal fungi, nitrogen-fixing bacteria, and some free-living 

microbes that have co-evolved with plants that supply nutrients to and obtain food from their 

 

1

Prokaryotes are, for the most part, single celled organisms that lack a nucleus or other membrane-bound organelles 

and include bacteria and archaea.

2 Organisms that are able to obtain food without the need for a host organism.
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plant hosts (USDA-NRCS, 2004). Legumes have developed symbiotic relationships with 

specific nitrogen-fixing bacteria in the family Rhizobiaceae that induce the formation of root 

nodules where bacteria may carry out the reduction of atmospheric nitrogen into ammonia (NH3) 

that is usable by the plant (Gage, 2004). Bradyrhizobium japonicum is the rhizobium bacteria 

specifically associated with soybeans (Franzen, 1999). Since neither soybean nor B. japonicum 

is native to North America, if a field has not been planted with soybean within three to five 

years, either the seed or seed zone must be inoculated with B. japonicum prior to soybean 

planting (Berglund and Helms, 2003; Pedersen, 2007). 

In addition to beneficial microorganisms, there are also several microbial pathogens that cause 

disease in soybean and vary somewhat depending on the region. These include fungal pathogens 

such as Rhizoctonia Stem Rot (Rhizoctonia solani), Brown Stem Rot (Phialophora gregata), 

Sudden Death Syndrome (Fusarium solani race A), and Charcoal Root Rot (Macrophomina 

phaseolina); bacterial pathogens Bacterial Blight (Pseudomonas syringae) and Bacterial Pustule 

(Xanthomonas campestri); and viral pathogens Soybean Mosaic Virus and the Tobacco Ringspot 

Virus (Ruhl, 2007; SSDW, No Date). The Soybean Cyst Nematode (Heterodera glycines) is a 

microscopic parasite that infects the roots of soybeans. Management to control disease outbreaks 

varies by region, and pathogen, and parasite, but include common practices such as crop rotation, 

weed control, planting resistant cultivars, and proper planting and tillage practices.

Herbicides

Herbicides have a wide variety of formulations, constituents, and recommended uses and 

concentrations that play a role in how herbicides affect microorganism communities. 

Understanding and quantifying the effects of their use is further compounded by differences in 

environment factors, including the main factors affecting microbial population size and diversity. 

As mentioned previously, some types of soil microorganisms share metabolic pathways with 

plants, and might be negatively affected by herbicides. Alternatively, many microorganisms feed 

on herbicides or produce enzymes that break-down herbicides (Tu et al., 2001; Haney et al., 

2002; Araujo et al., 2003; Senseman, 2007; US-EPA, 2008b). This microorganism activity is 

instrumental to herbicide degradation in the soil. As a result, herbicides have both positive and 

negative effects on microorganism groups that may increase the population of some while 

reducing the population of others.

4.5.1.4 No Action Alternative - Biological Diversity

Biodiversity refers to all plants, animals, and microorganisms interacting in an ecosystem 

(Wilson, 1988). Biodiversity provides valuable genetic resources for crop improvement (Harlan, 

1975) and also provides other functions beyond food, fiber, fuel, and income. These include 

pollination, genetic introgression, biological control, nutrient recycling, competition against 

natural enemies, soil structure, soil and water conservation, disease suppression, control of local 

microclimate, control of local hydrological processes, and detoxification of noxious chemicals 

(Altieri, 1999). The loss of biodiversity can result in a need for costly management practices in 

order to provide these functions to the crop (Altieri, 1999). 

The degree of biodiversity in an agroecosystem depends on four primary characteristics: (1) 

diversity of vegetation within and around the agroecosystem; (2) permanence of various crops 

within the system; (3) intensity of management; and (4) extent of isolation of the agroecosystem 
Final BASF CV127 Soybean EA

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from natural vegetation (Altieri, 1999). Agricultural land subject to intensive farming practices, 

such as that used in crop production, generally has low levels of biodiversity compared with 

adjacent natural areas. Tillage, seed bed preparation, planting of a monoculture crop, pesticide 

use, fertilizer use, and harvesting limit the diversity of plants and animals (Lovett et al., 2003). 

Biodiversity can be maintained or reintroduced into agroecosystems through the use of woodlots, 

fencerows, hedgerows, and wetlands. Agronomic practices that may be employed to support 

biodiversity include intercropping (the planting of two or more crops simultaneously to occupy

the same field), agroforestry, crop rotations, cover crops, no-tillage, composting, green manuring 

(growing a crop specifically for the purpose of incorporating it into the soil in order to provide 

nutrients and organic matter), addition of organic matter (compost, green manure, animal 

manure, etc.), and hedgerows and windbreaks (Altieri, 1999). Integrated pest management 

strategies include several practices that increase biodiversity such as retaining small, diverse 

natural plant refuges and minimal management of field borders.

The potential impacts to biodiversity associated with the agricultural production of crops include 

a loss of diversity, which can occur at the crop, farm, and/or landscape level (Visser, 1998; 

Ammann, 2005; Carpenter, 2011). In this EA, crop diversity refers to the genetic uniformity 

within crops, farm-scale diversity refers to the level of complexity of organisms within the 

boundaries of a farm, and landscape level diversity refers to potential changes in land use and the 

impacts of area-wide weed suppression beyond the farm boundaries (Carpenter, 2011).

Crop Diversity

Genetic diversity in crops is beneficial as it may improve yields, pest and disease resistance, and 

quality in agricultural systems, and that greater varietal and species diversity enable growers to 

maintain productivity over a wide range of conditions (Krishna et al., 2009). There is concern 

that the adoption of GE technology potentially reduces grower-demand for crop genetic diversity

because breeding programs could concentrate on a smaller number of high value cultivars, which 

could reduce the availability of, and demand for, non-GE varieties (Carpenter, 2011). In 

contrast, several studies involving GE soybeans and cotton have found this not to be the case, 

indicating the introduction of GE crops has not decreased crop species diversity (Ammann, 2005; 

Carpenter, 2011). 

Concern for the loss of genetic variability has led to the establishment of a worldwide network of 

genebanks (van de Wouw et al., 2010). The USDA Soybean Germplasm Collection, which is 

part of the National Plant Germplasm System, acquires, maintains, and evaluates soybean 

germplasm and distributes seed samples to scientists in 35 states (University of Illinois, 2003). 

Nationwide, there are over 23,190 soybean varieties (USDA-ARS, 2013) that provide a vast 

reservoir of genetic diversity for crop development.

Farm-scale Diversity

As noted previously, agricultural practices have the potential to impact diversity at the farm level 

by affecting a farm’s biota, including birds, wildlife, invertebrates, soil microorganisms, and 

weed populations. For example, an increase in adoption of conservation tillage practices is 

associated with the use of GE herbicide-resistant crops (Givens et al., 2009). Less tillage 

provides more wildlife habitat by allowing other plants to establish between crop rows in either 

stubble or weeds. Conservation tillage also leaves a higher rate of plant residue and increases 

soil organic matter (Hussain et al., 1999), which benefit soil biota by providing additional food 
Final BASF CV127 Soybean EA

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sources (energy) (USDA-NRCS, 1996) and increase the diversity of soil microorganisms, as 

discussed in Subsection 4.5.1, Biological Resources, No Action Alternative – Soil 

Microorganisms. In addition, invertebrates that feed on plant detritus and their predators and, in 

turn, birds and other wildlife that prey on them, may benefit from increased conservation tillage 

practices (Towery and Werblow, 2010; Carpenter, 2011). Ground-nesting and seed-eating birds, 

in particular, have been found to benefit from greater food and cover associated with 

conservation tillage (SOWAP, 2007).

Herbicide use in agricultural fields may impact biodiversity by decreasing weed quantities or 

causing a shift in weed species present in the field, which would affect those insects, birds, and 

mammals that feed on or find shelter in these weeds. The quantity and type of herbicide use 

associated with conventional and GE crops is dependent on many variables, including cropping 

systems, type and abundance of weeds, production practices, and individual grower decisions.

Landscape-scale Diversity

The greatest direct impact of agriculture on biodiversity on the landscape scale results from the 

loss of natural habitats caused by the conversion of natural ecosystems into agricultural land 

(Ammann, 2005). Increases in crop yields, such as has been observed in the last 10 years in 

soybean production, have the potential to reduce impacts to biodiversity by allowing less land to 

be converted to agriculture than would otherwise be necessary (Carpenter, 2011); however, 

substantial gains in yields have generally not been obtained by herbicide-resistant cultivars 

unless higher yielding cultivars are modified with an herbicide-resistant trait (NRC, 2010). 

Similar to that discussed in farm-scale diversity, the use of herbicides at the landscape-level also 

has the potential to impact biodiversity. Increased conservation tillage practices associated with 

herbicide-resistant crops over large areas may increase certain populations of invertebrates and 

wildlife that benefit from conservation tillage, whereas those species dependent on the targeted 

weeds may be negatively impacted. Potential impacts to landscape-scale diversity can also be 

related to the effects of herbicides on non-target plant and animal species.

Several recent studies (Hartzler, 2010; Brower et al., 2012; Pleasants and Oberhauser, 2012)

have examined the potential causes of observed decreases in overwintering monarch butterfly 

(Danaus plexippus) populations, namely the reduced infestations of common milkweed 

(Asclepias syriaca), a perennial weed, in Corn Belt agricultural fields. The loss of host milkweed 

plants in agricultural fields is assumed to be a result of the increased use of glyphosate associated 

with the high adoption rate of GE crops (Brower et al., 2012), although slight declines in 

milkweed abundance in non-agricultural areas not related to glyphosate use were also observed. 

However, it was concluded that the observed reduced monarch abundance is likely based on 

several contributing factors including: degradation of the forest in the overwintering areas; the 

loss of breeding habitat (i.e., milkweed host plants) in the U.S., resulting from the use of 

herbicide associated with the expansion of GE herbicide-resistant crop acreage and from 

continued land development; and severe weather (Hartzler, 2010; Brower et al., 2012; Pleasants 

and Oberhauser, 2012).

While herbicide use potentially affects biodiversity, the application of pesticides in accordance 

with EPA-registered label uses and careful management of chemical spray drift minimizes the 

potential biodiversity impacts from their use.
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4.5.2 Preferred Alternative – Biological Resources

Because the regulatory decision on the petition for nonregulated status of BASF CV127 Soybean

will not change the availability of soybean varieties or the agronomic practices associated with 

these soybean varieties, there will be no change to biological resources when compared to the No 

Action Alternative. Herbicide use in the U.S. is limited to those applications described on the

EPA-approved labels. This soybean is engineered to be resistant to an herbicide that is not been 

approved by the EPA for use on soybeans and no application is pending or planned. Therefore, 

if these soybeans were acquired by a U.S. grower, they would be cultivated in the same way as 

currently available varieties. Because the effects on these resources result from the agronomic 

practices and these are same under the no action and the preferred alternative, there is no change 

to animal or plant communities, microorganisms, or biological diversity associated with this 

decision.

The AtAHAS protein associated with the GE modification of this variety is derived from 

Arabidopsis thaliana, and the AHAS proteins are ubiquitous in plants, thus animals are routinely 

exposed to this protein (BASF, 2011; BASF, 2012 ). The AHAS proteins are not known to be 

toxic or allergenic, and have a long history of safe consumption in food and feed products from 

many different Clearfield®crops that contain these proteins (BASF, 2011). 

BASF has presented the results of analytic studies comparing the AtAHAS protein with the 

AHAS proteins found in the Clearfield®varieties, and has found no functional differences in 

structure or function (BASF, 2011). BASF has presented evidence that these proteins do not 

differ in their digestibility, toxicity and allergenicity (BASF, 2011; BASF, 2012 ). The 

introduced genetic material does not result in the production of novel proteins, enzymes, or 

metabolites in the plant that are known to have toxic properties (USDA-APHIS, 2012). 

Moreover, BASF has also presented the results of a feeding study in which mice were fed very 

high doses of AtAHAS protein, with no toxic effects (BASF, 2011; BASF). Therefore, 

consumption of CV127 soybeans by animals in the field will not affect animals differently than 

the consumption of soybeans under the No Action Alternative.

EPA has responsibility to regulate the use of pesticides (herbicides) that may be used on feed 

crops, and must establish pesticide tolerances (maximum pesticide residue levels) for the amount 

of pesticide residue that can legally remain in or on the feed crop. EPA undertakes this analysis 

under the authority of the FFDCA, and must conclude that such tolerances will be safe, meaning 

that there is a reasonable certainty that no harm to human health will result from the use of the 

pesticide.

5 CUMULATIVE IMPACTS

5.1 ASSUMPTIONS USED FOR CUMULATIVE IMPACTS ANALYSIS

Cumulative effects have been analyzed for each environmental issue assessed in Section 4, 

Environmental Consequences. The cumulative effects analysis is focused on the incremental 

impacts of the Preferred Alternative taken in consideration with related activities including past, 

present, and reasonably foreseeable future actions. In this analysis, if there are no direct or 

indirect impacts identified for a resource area, then APHIS assumes there can be no cumulative 
Final BASF CV127 Soybean EA

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impacts. Where it is not possible to quantify impacts, APHIS provides a qualitative assessment 

of potential cumulative impacts. APHIS considered the potential for BASF CV127 Soybean

to extend the range of soybean production and affect the conversion of land to agricultural 

purposes. BASF’s studies demonstrate BASF CV127 Soybean is similar in its growth

habit, agronomic properties, disease susceptibility to other nonregulated varieties of soybean

(BASF, 2011; USDA-APHIS, 2012). This implies that its cultural requirements would 

neither differ from those of other soybeans nor change the areas in which soybeans are

currently cultivated. BASF does not intend to market this soybean in the U.S. so it is not 

likely to be available to growers. As such, land use changes associated with approving the 

petition for nonregulated status to BASF CV127 Soybean is not expected to influence the 

use of current soybean cultivars. Therefore, although the preferred alternative would allow 

for new plantings of BASF CV127 Soybean to occur anywhere in the U.S., actual planting 

would likely be limited to breeding plots or production of seed for planting in the target market. 

Because imazypyr and imazapic are not labeled for use on soybeans and there is no label 

pending, any BASF CV127 Soybeans grown in the U.S. could not be cultivated using these 

herbicides even though the plants are resistant to the herbicides. 

5.2 PAST AND PRESENT ACTIONS 

In the preceding analysis, the potential impacts from approving the petition for nonregulated

status to BASF CV127 Soybean were assessed. The potential impacts under the Preferred 

Alternative for all the resource areas analyzed were the same as those described for the Noaction Alternative. 

The Preferred Alternative is not expected to directly cause a measurable change in agricultural 

acreage or area devoted to conventional or GE-soybean cultivation or soybean grown for seed in

the U.S. The majority of soybean grown in the U.S. is GE and herbicide resistant. Because 

BASF CV127 Soybean will not be marketed in the U.S. it will not affect current soybean 

planting, management practices, or cultivation. 

Based upon recent trends, adding GE varieties to the market is not related to the ability of 

organic production systems to maintain their market share. As described above, the majority of 

soybean is herbicide resistant. Since 1993, 11 GE soybean events or lines have been determined 

by APHIS to be no longer subject to the regulatory requirements of 7 CFR part 340 or the plant 

pest provisions of the PPA(USDA-APHIS, 2013). U.S. organic soybean production acreage is 

less than 100,000 acres (USDA-ERS, 2010). Approving the petition for BASF CV127 Soybean

is not expected to impact the organic production of soybean. It is not likely to be grown 

extensively in the U.S. and the majority of the soy in the U.S. is GE.

Approving the petition for a determination of nonregulated status to BASF CV127 Soybean is 

not expected to result in changes to current soybean cropping practices. Studies conducted by 

BASF demonstrate that, in terms of agronomic characteristics and cultivation practices, BASF 

CV127 Soybean is similar to other soybean varieties currently grown (BASF, 2011; USDAAPHIS, 2012). Consequently, no changes to current soybean cropping practices such as tillage, 

crop rotation, or agricultural inputs associated with the adoption of BASF CV127 Soybean are 

expected if it were to be grown in the U.S. 
Final BASF CV127 Soybean EA

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Approving the petition for a determination of nonregulated status to BASF CV127 Soybean

would have the same impacts to water, soil, air quality, and climate change as the no action 

alternative. Agronomic practices that have the potential to impact soil, water and air quality, and 

climate change such as tillage, agricultural inputs (fertilizers and pesticides), and irrigation 

would not change because BASF CV127 Soybean is agronomically similar to other soybeans 

and the herbicides (imazapyr and imazapic) to which it is resistant are not labeled for use on the 

plant in the U.S. (BASF, 2011; BASF, 2012 ). 

The impacts of the Preferred Alternative to animal and plants communities, microorganisms, and 

biodiversity would be no different than that experienced under the No-action Alternative. BASF 

CV127 Soybean is both agronomically and compositionally similar to other soybean. Thus, it 

would not require any different agronomic practices to cultivate, and does not represent a

weediness risk that is any different from other currently available soybean. Availability of 

BASF CV127 Soybean would not impact the development of herbicide resistant weeds or the 

trend to broaden weed management tactics to affect control over herbicide resistant weeds, as it 

was not developed to be marketed in the U.S. (BASF, 2011; BASF, 2012 ). 

There are no differences in the potential for gene flow and weediness under the Preferred Action 

Alternative. Outcrossing and weediness are addressed in the PPRA (USDA-APHIS, 2012). 

BASF CV127 Soybean is similar to other soybean varieties. The risk of gene flow and 

weediness of BASF CV127 Soybean is no greater than that of other soybeans.

Food and feed derived from GE soybean must be in compliance with all applicable legal and 

regulatory requirements and may undergo a voluntary consultation process with the FDA prior to 

release onto the market to identify and discuss relevant safety, nutritional, or other regulatory

issues regarding the bioengineered food. BASF CV127 Soybean is expected to have no toxic 

effect to human health or livestock. BASF submitted a safety and nutritional assessment and 

food safety consultation of BASF CV127 Soybean was completed by the FDA (BNF No. 114) 

on February 17, 2012. No change in food and feed safety is expected to occur under the 

Preferred Alternative.

BASF CV127 Soybean is not expected to affect the seed, feed, and food trade BASF CV127

soybeans. BASF CV127 Soybean does not require different agricultural management practices 

or agronomic inputs than from conventional soybean (BASF, 2011; BASF, 2012 ) . These 

agronomic and management similarities strongly suggest that coexistence strategies already 

adopted to protect and preserve soybean varietal integrity in the marketplace will not be required 

to change to accommodate the cultivation of BASF CV127 Soybean. 

In summary, the potential cumulative effects regarding past and present actions combined with 

the Preferred Alternative have been analyzed, and no changes from the current baseline under the 

No-action Alternative would occur. 

5.3 REASONABLY FORESEEABLE ACTIONS

BASF has stated in the petition that it does not intend to commercialize BASF CV127 Soybean 

in the U.S. (BASF, 2011; BASF, 2012 ). Under the preferred alternative, APHIS assumes that 

growers could plant BASF CV127 soybeans, if they were available. Growers could use any
Final BASF CV127 Soybean EA

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management practices that are suitable for the production of soy. Growers could use EPA

registered herbicides on these soybeans. However, growers could not use imazapyr because it is 

not labeled for use on soybeans, and there is no label for use pending. According to the 

developer, there is a very small market in the U.S.; the projected market would not support the 

cost associated with pursuing the change in EPA registration (BASF, 2011). 

It is likely that BASF CV127 Soybean will be adopted by growers in Brazil and Argentina. As 

discussed in Section 4.2.1 - Socioeconomic - No Action Alternative: Soybean Imports and 

Exports, BASF has submitted applications to several countries where this crop could be 

cultivated or consumed as food or feed. The U.S. imports about 6% of its imported soybeans 

from South America, mainly from Argentina, and therefore it is possible that BASF CV127

could be imported into the U.S. To facilitate foreign production and possible importation, BASF 

has applied to the EPA for an import residue tolerance for the use of imazapyr and imazapic on 

soybean (BASF, 2011).

As described in Section 4 – Environmental Consequences, the potential for impacts of BASF 

CV127 Soybean would not result in any changes to the resources areas when compared to the No

Action Alternative. No cumulative effects are expected from approving the petition for 

nonregulated status for BASF CV127 Soybean, when taken in consideration with related 

activities, including past, present, and reasonably foreseeable future actions. 
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6 THREATENED AND ENDANGERED SPECIES

6.1 USDA-APHIS’ APPROACH TO EVALUATION OF POTENTIAL IMPACTS TO 

THREATENED AND ENDANGERED SPECIES

Congress passed the Endangered Species Act (ESA) of 1973, as amended, to prevent extinctions 

facing many species of fish, wildlife, and plants. The purpose of the ESA is to conserve 

threatened and endangered species (TES) and the ecosystems on which they depend as key

components of America’s heritage. To implement the ESA, the U.S. Fish and Wildlife Service 

(US-FWS) works in cooperation with the National Marine Fisheries Service (NMFS); other 

Federal, State, and local agencies; Tribes; non-governmental organizations; and private citizens. 

Before a plant or animal species can receive the protection provided by the ESA, it must first be 

added to the Federal list of threatened and endangered wildlife and plants.

A species is added to the list when it is determined by the US-FWS/NMFS to be endangered or 

threatened because of any of the following factors:

 The present or threatened destruction, modification, or curtailment of its habitat or 

range;

 Overutilization for commercial, recreational, scientific, or educational purposes;

 Disease or predation;

 The inadequacy of existing regulatory mechanisms; and

 The natural or manmade factors affecting its survival.

Once an animal or plant is added to the list, in accordance with the ESA, protective measures 

apply to the species and its habitat. These measures include protection from adverse effects of 

Federal activities. 

Section 7 (a) (2) of the ESA requires that Federal agencies, in consultation with US-FWS and/or 

the NMFS, ensure that any action they authorize, fund, or carry out is not likely to jeopardize the 

continued existence of a listed species or result in the destruction or adverse modification of 

designated critical habitat. It is the responsibility of the Federal agency taking the action to assess 

the effects of their action and to consult with the US-FWS and/or NMFS if it is determined that 

the action “may affect” listed species or critical habitat. To facilitate USDA-APHIS’ ESA 

consultation process, USDA-APHIS met with the US-FWS from 1999 to 2003 to discuss factors 

relevant to USDA-APHIS’s regulatory authority and effects analysis for petitions for 

nonregulated status, and developed a process for conducting an effects determination 

consistent with the PPA (Title IV of Public Law 106-224). USDA-APHIS uses this process 

to help fulfill its obligations and responsibilities under Section 7 of the ESA for 

biotechnology regulatory actions.

USDA-APHIS’ regulatory authority over GE organisms under the PPA is limited to those GE 

organisms for which it has reason to believe might be a plant pest or those for which USDAAPHIS does not have sufficient information to determine that the GE organism is unlikely to 

pose a plant pest risk (7 CFR §340.1). USDA-APHIS does not have authority to regulate the use 

of any herbicide, including imidazolinone herbicides. After completing a PPRA, if USDA-
Final BASF CV127 Soybean EA

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APHIS determines that BASF CV127 Soybean does not pose a plant pest risk, then BASF 

CV127 Soybean would no longer be subject to the plant pest provisions of the PPA or to the 

regulatory requirements of 7 CFR §340, and therefore, USDA-APHIS must reach a 

determination that the article is no longer regulated. As part of its EA analysis, USDA-APHIS is 

analyzing the potential effects of BASF CV127 Soybean on the environment including any

potential effects to threatened and endangered species and critical habitat. As part of this process, 

USDA-APHIS thoroughly reviews the genetically engineered product information and data 

related to the organism (generally a plant species, but also may be other genetically engineered 

organisms). As described in Appendix D, for each transgene/transgenic plant, USDA-APHIS 

considers the following: 

 Reviews of the biology and taxonomy of the crop plant and its sexually compatible 

relatives;

 Characterization of each transgene with respect to its structure and function and the 

nature of the organism from which it was obtained;

 Location(s) of the new transgene and its products (if any) produced in the plant and 

their quantity;

 Reviews of the agronomic performance of the plant including disease and pest 

susceptibilities, weediness potential, and agronomic and environmental impact;

 Concentrations of any known plant toxicants, if applicable; 

 Sexual compatibility of the transgenic plant with any threatened or endangered 

species (TES) of plants or a host of any TES; and

 Any other information that may inform the potential for an organism to pose a plant 

pest risk.

As noted in Subsection 1.2 – Purpose of Product, BASF has developed this product to provide 

growers in Brazil and Argentina with an alternative herbicide-resistant soybean product. As 

noted in Section 4 – Environmental Consequences and elsewhere in this EA, it is highly unlikely 

that BASF CV127 Soybean will be commercially cultivated in the U.S. Despite this expectation, 

USDA-APHIS presents the following analysis of the potential impacts of TES in the unlikely

event that BASF CV127 Soybean is cultivated in a very limited commercial setting.

6.2 SCOPE OF APHIS’ EFFECTS ANALYSIS OF THE CULTIVATION OF BASF CV127 

SOYBEAN ON TES

In following its review process, USDA-APHIS, as described below, has evaluated the potential 

effects that approval of the petition for nonregulated status of BASF CV127 Soybean plants may

have, if any, on Federally-listed TES and species proposed for listing, as well as designated 

critical habitat and habitat proposed for designation. 

Studies performed by BASF demonstrate that agronomic characteristics and cultivation practices 

required for BASF CV127 Soybean are essentially indistinguishable from practices used to grow 

other soybean varieties, including other herbicide-resistant varieties (BASF, 2011; USDAAPHIS, 2012; BASF, 2012 ). 

As discussed in Subsection 4.1 – Scope of the Environmental Analysis, commercial cultivation 

of BASF CV127 Soybean in the U.S. is expected to be very limited. If cultivated at all, BASF 
Final BASF CV127 Soybean EA

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CV127 Soybean will likely only be cultivated in controlled small research and development plots 

and small off-season seed cultivation (BASF, 2011). As discussed in Subsection 4.2.2 –

Preferred Alternative - Areas and Acreage of Soybean Production, USDA-APHIS has 

determined BASF CV127 Soybean to be unlikely to extend the range of soybean production, and 

new acreage is not expected to be developed to accommodate the cultivation of BASF CV127 

Soybean. In addition, BASF CV127 Soybean is not expected to replace other varieties of 

soybean currently cultivated (BASF, 2011; BASF, 2012 ).

USDA-APHIS met with US-FWS officials on June 15, 2011 to discuss whether USDA-APHIS 

has any obligations under the ESA regarding analyzing the impacts of herbicide use associated 

with all GE crops on TES. As a result of these joint discussions, US-FWS and USDA-APHIS 

have agreed that it is not necessary for USDA-APHIS to perform an ESA effects analysis on 

herbicide use associated with GE crops because US-EPA has both regulatory authority over the 

labeling of pesticides and the necessary technical expertise to assess pesticide effects on the 

environment under the FIFRA. USDA-APHIS has no statutory authority to authorize or regulate 

the use of imidazolinone herbicides, or any other herbicide, by soybean growers. Under USDAAPHIS’ current CFR Part 340 regulations, USDA-APHIS only has the authority to regulate 

BASF CV127 Soybean or any GE organism as long as USDA-APHIS believes it may pose a 

plant pest risk. For GE organisms, USDA-APHIS has no regulatory jurisdiction over any other 

risks associated with GE organisms including risks resulting from the use of herbicides or other 

pesticides on those organisms. As stated in Section 5 – Cumulative Impacts - because imazypyr 

and imazapic are not labeled for use on soybeans, any BASF CV127 Soybeans grown in the U.S. 

could not be cultivated using these herbicides even though the plants are resistant to the 

herbicides. Moreover, BASF has no intention of seeking a label amendment to provide for the 

application of these herbicides to BASF CV127 Soybean (BASF, 2011). If in the future BASF 

or others decided to seek a label amendment, US-EPA would evaluate the request under their 

Endangered Species Pesticide Program (http://www.epa.gov/oppfead1/endanger/basic-info.htm). 

Under the program, if it is determined that the action may affect TES or critical habitat, the USEPA would consult with US-FWS and/or the NMFS as required by Section 7 of the ESA. 

USDA-APHIS obtained and reviewed the US-FWS list of TES species (listed and proposed). 

The source for USDA-APHIS’ review was the US-FWS Environmental Conservation Online 

System (US-FWS, 2013). 

USDA-APHIS focused its TES effects analysis on the implications of exposure to the AHAS 

protein and csr1-2 gene in soybean, the interaction between TES and the BASF CV127 Soybean 

plant including potential for sexual compatibility, and ability to serve as a host for a TES.

6.3 POTENTIAL EFFECTS OF BASF CV127 SOYBEAN ON TES

BASF CV127 Soybean is not sexually compatible with any listed TES plant species or plant 

proposed for listing; none of these listed plants are in the same genus nor are known to cross 

pollinate with species of the genus Glycine.

USDA-APHIS considered the possibility that BASF CV127 Soybean could serve as a host plant 

for a TES species. A review of the species list reveals that there are no members of the genus 

Glycine that serve as a host plant for any TES.
Final BASF CV127 Soybean EA

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BASF has presented data evaluating the agronomic and morphological characteristics of BASF 

CV127 Soybean, as well as compositional and nutritional characteristics, and safety evaluations 

and toxicity tests, comparing the product to a nontransgenic isoline control (BASF, 2011; BASF, 

2012 ). Compositional elements compared included moisture, protein, fat, carbohydrates, ash, 

minerals, dietary fiber, essential and non-essential amino acids, fatty acids, vitamins, and antinutrients (BASF, 2011; BASF, 2012 ). Allergenicity and toxicity studies included bioinformatics 

analyses, digestibility and acute protein toxicity studies (BASF, 2011; BASF). No biologically 

meaningful differences were observed when comparing BASF CV127 Soybean with the near 

isoline variety (BASF, 2011; USDA-APHIS, 2012; BASF, 2012 ). BASF CV127 Soybean does 

not appear to present any changes in agronomic inputs, morphological characteristics or 

composition and nutritional characteristics that would affect TES. 

The AHAS protein and csr1-2 gene in BASF CV127 Soybean are derived from Arabidopsis 

thaliana (BASF, 2011; BASF, 2012 ). The Arabidopsis AHAS (AtAHAS) is a member of the 

class of AHAS proteins found ubiquitously in plants (BASF, 2012 ).

In its petition, BASF has presented the results of laboratory assays in which non-target 

organisms were exposed to the AHAS proteins, as well as the results of bioinformatics studies 

evaluating toxicity and allergenicity of the AHAS proteins (BASF, 2011). The introduced 

genetic material does not result in the production of novel proteins, enzymes, or metabolites in 

the plant that are known to have toxic properties (BASF, 2012 ). The lack of known toxicity of 

AtAHAS enzyme suggests no potential for deleterious effects on threatened and endangered 

organisms (BASF, 2012 ). Information presented in BASF’s petition support the conclusion that 

there are no biologically meaningful differences between BASF CV127 Soybean and 

commercially available soybean products. 

The potential for gene movement between BASF CV127 Soybean and related soybean 

species is limited (BASF, 2011; USDA-APHIS, 2012; BASF, 2012 ). As discussed in USDAAPHIS’ PPRA, cultivated soybean is highly self-pollinating (Ahrent and Caviness, 1994).

When soybean plants are grown directly adjacent to other soybean plants, the amount of natural 

cross pollination has generally been found to be 0.5 - 1% (OECD, 2000; Fehr, 2007) although 

higher values (up to 2.5%) have been noted in some varieties (Abud et al., 2003). Outcrossing 

can be reduced to 0 – 0.01% with a separation distance of 10 meters (Abud et al., 2007; USDAAPHIS, 2012). 

The cultivated soybean, G. max, lacks sexually compatible wild relatives in the U.S. and its 

territories (USDA-APHIS, 2012). Consequently, there is no potential for gene movement from 

cultivated soybean plants to wild relatives in the U.S. Therefore, it is not likely that gene 

movement and introgression will occur between BASF CV127 Soybean and other species of 

soybean. 

BASF CV127 Soybean does not present a potential as a weed or the potential to displace a TES. 

As discussed in the PPRA, soybean lacks the attributes commonly associated with weeds, such 

as persistence of seed in soil, the ability to disperse, invade or become a dominant species in new 

or diverse landscapes, or the ability to compete with native species (Baker, 1965; USDA-APHIS, 

2012). The agronomic and morphologic characteristics data provided by BASF were used in the 

USDA-APHIS analysis of the weediness potential for BASF CV127 Soybean, and evaluated for 
Final BASF CV127 Soybean EA

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the potential to impact TES. Agronomic studies conducted by BASF tested the hypothesis that 

the weediness potential of BASF CV127 Soybean is unchanged with respect to conventional 

soybean (BASF, 2011; USDA-APHIS, 2012; BASF, 2012 ). No differences were detected 

between BASF CV127 Soybean and nontransgenic isoline control in growth, reproduction, or 

interactions with pests and diseases, other than the intended effect of herbicide-resistance

(USDA-APHIS, 2012). Based on the agronomic field data and literature survey on soybean 

weediness potential, BASF CV127 Soybean is unlikely to affect TES as a troublesome or 

invasive weed (BASF, 2011; USDA-APHIS, 2012; BASF, 2012 ). 

In addition to evaluating BASF’s comparisons of BASF CV127 Soybean with the non-transgenic 

near-isoline control also considers the US-EPA and US-FDA regulatory assessments in its 

environmental assessment. As discussed above in Cumulative Effects, Past and Present Actions 

(Subsection 5.2), BASF has completed a food and feed safety and nutritional assessments and 

consultation for BASF CV127 Soybean with the US-FDA. There are currently over 24 tolerances 

for residues of the imidazolinone herbicides; BASF has submitted an import tolerance petition 

and supporting residue data to the US-EPA for the use of imazapyr and imazapic on BASF 

CV127 Soybean (BASF, 2011). 

As discussed above, cultivation of BASF CF127 Soybean in the US is unlikely, but if it were to 

occur, it would be limited to research and development plots and off-season seed production

(BASF, 2011). BASF CV127 Soybean are not expected to replace other varieties of soybean 

currently cultivated in the U.S. (BASF, 2011; BASF, 2012 ). Moreover, as noted in Subsection 

4.2.2 – Preferred Alternative - Areas and Acreage of Soybean Production, USDA-APHIS has 

determined that BASF CV127 Soybean is unlikely to extend the range of soybean production, 

and new acreage is not expected to be developed to accommodate the cultivation of BASF 

CV127 Soybean. 

After reviewing the possible effects of the approval of the petition for nonregulated status for

BASF CV127 Soybean, USDA-APHIS has not identified any stressor that could affect the 

reproduction, numbers, or distribution of a listed TES or species proposed for listing. As a result, 

a detailed exposure analysis for individual species is not necessary. USDA-APHIS also 

considered the potential effect of approval of a petition for nonregulated status of BASF CV127 

Soybean on designated critical habitat or habitat proposed for designation. Soybean has been 

selected for domestication and cultivation under conditions not normally found in natural settings 

(OECD, 2000). USDA-APHIS could identify no differences from effects that would occur from 

the cultivation of BASF CV127 Soybean and other currently cultivated soybean varieties. 

Based on these factors, USDA-APHIS has concluded that the approval of the petition for 

nonregulated status of BASF CV127 Soybean, and the corresponding environmental release of 

this soybean variety, will have no effect on listed species or species proposed for listing, and 

would not affect designated habitat or habitat proposed for designation. Because of this no-effect 

determination, consultations under Section 7(a)(2) of the Act or the concurrences of the US-FWS

and/or NMFS are not required. 
Final BASF CV127 Soybean EA

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7 CONSIDERATION OF EXECUTIVE ORDERS, STANDARDS, AND 

TREATIES RELATING TO ENVIRONMENTAL IMPACTS

7.1 EXECUTIVE ORDERS WITH DOMESTIC IMPLICATIONS

The following two executive orders require consideration of the potential impacts to minority

and low income populations and children:

 EO 12898 (US-NARA, 2010), "Federal Actions to Address Environmental Justice in 

Minority Populations and Low-Income Populations," requires Federal agencies to 

conduct their programs, policies, and activities that substantially affect human health or 

the environment in a manner so as not to exclude persons and populations from 

participation in or benefiting from such programs. It also enforces existing statutes to 

prevent minority and low-income communities from being subjected to 

disproportionately high and adverse human health or environmental effects.

 EO 13045, “Protection of Children from Environmental Health Risks and Safety 

Risks,” acknowledges that children may suffer disproportionately from environmental 

health and safety risks because of their developmental stage, greater metabolic activity

levels, and behavior patterns, as compared to adults. The EO (to the extent permitted by

law and consistent with the agency’s mission) requires each Federal agency to identify, 

assess, and address environmental health risks and safety risks that may 

disproportionately affect children.

Each Alternative was analyzed with respect to EO 12898 and EO 13045. Neither Alternative is 

expected to have a disproportionate adverse effect on minorities, low-income populations, or 

children. 

As discussed in Subsection 4.1 – Scope of the Environmental Analysis, BASF does not intend to 

make BASF CV127 Soybean available for cultivation in the U.S. Therefore, it is highly unlikely 

that minority populations, low income populations, and children will be exposed to the BASF 

CV127 Soybean. To the extent BASF CV127 Soybean is cultivated in the U.S., as discussed in

Sections 4 and 5, U.S. cultivation would be limited to small research and development plots, a 

small volume of off-season seed production, and a very limited cultivation in those soybean 

production areas where weeds resistant to the imidazolinone herbicides are not found. These 

scenarios are deemed highly improbable. Moreover, BASF has indicated that such cultivation 

would require a change in the US-EPA’s pesticide registration label, and have further asserted 

that BASF has no intention to seek such a label change. Based on these findings, approval of the 

petition for nonregulated status for BASF CV127 Soybean is not expected to have a 

disproportionate adverse effect on minorities, low income populations, or children. 

The following EO addresses Federal responsibilities regarding the introduction and effects of 

invasive species:

 EO 1311 (US-NARA, 2010), “Invasive Species,” states that Federal agencies take 

action to prevent the introduction of invasive species, to provide for their control, and to 

minimize the economic, ecological, and human health impacts that invasive species 

cause.
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As discussed in Subsections 3.1.1 and 4.2.1 – Areas and Acreage of Soybean Production, nonengineered soybean and soybean engineered for herbicide-resistance are widely grown in the 

U.S.. Based on historical experience with these varieties and the data submitted by the applicant 

and reviewed by USDA-APHIS, BASF CV127 Soybean plants are sufficiently similar in fitness 

characteristics to other soybean varieties currently grown and are not expected to become weedy

or invasive (USDA-APHIS, 2012). 

The following Treaty, Statute and EO require the protection of migratory bird populations:

 Migratory Bird Treaty Act of 1918 (MBTA), (16 U.S.C. 703 – 712) implements a 

1916 Convention between the U.S. and Great Britain (now Canada) for the protection 

of migratory birds (USDA-FSA, 2012). Later amendments implemented treaties 

between the U.S. and Mexico, the U.S. and Japan, and the U.S. and Russia. The 

MBTA establishes a Federal prohibition to “pursue, hunt, take, capture, kill, attempt 

to take, capture or kill, possess, offer for sale, sell, offer to purchase, purchase, deliver 

for shipment, ship, cause to be shipped, deliver for transportation, transport, cause to 

be transported, carry, or cause to be carried by any means whatever, receive for 

shipment, transportation or carriage, or export, at any time, or in any manner, any 

migratory bird, included in the terms of this Convention . . . for the protection of 

migratory birds . . . or any part, nest, or egg of any such bird." (16 U.S.C. 703). The 

Secretary of the Interior has the authority to determine, consistent with the 

Conventions, when "hunting, taking, capture, killing, possession, sale, purchase, 

shipment, transportation, carriage, or export of any . . .bird, or any part, nest or egg" 

could be undertaken and to adopt regulations for this purpose. These determinations 

are to be made based on "due regard to the zones of temperature and to the 

distribution, abundance, economic value, breeding habits, and times of migratory

flight." (16 U.S.C. 704). A list of birds protected by the U.S. Fish and Wildlife 

Service under the MBTA can be found at the following website: 

http://www.fws.gov/migratorybirds/RegulationsPolicies/mbta/mbtandx.html. Federal 

agency implementation of the MBTA is guided by EO 13186, described in the 

following bullet.

 EO 13186 (US-NARA, 2010), “Responsibilities of Federal Agencies to Protect 

Migratory Birds,” states that Federal agencies taking actions that have, or are likely 

to have, a measurable negative effect on migratory bird populations are directed to 

develop and implement, within two years, a Memorandum of Understanding (MOU) 

with the Fish and Wildlife Service that shall promote the conservation of migratory bird 

populations. 

As discussed in Subsection 4.3, Preferred Alternative - Human Health and Animal Feed, data 

submitted by the applicant has shown no difference in compositional and nutritional quality 

of BASF CV127 Soybean compared to other GE soybean or non-GE-soybean. As noted 

above in the discussion regarding minorities, low income populations and children, BASF 

does not intend to seek commercial cultivation of BASF CV127 Soybean in the U.S. To the 

extent that this soybean is cultivated domestically, such cultivation would be limited to 

small research and development plots, off-season seed production, and limited cultivation in 

those soybean production areas where weeds resistant to the imidazolinone herbicides are not 

found. As stated above, these scenarios are deemed highly unrealistic. Moreover, BASF has 

indicated that such cultivation would require a change in the US-EPA’s pesticide registration 
Final BASF CV127 Soybean EA

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label, and have further asserted that BASF has no intention to seek such a label change. 

Additionally, BASF has presented results of field trials conducted to evaluate field 

phenotypic, agronomic and environmental interactions associated with BASF CV127 

Soybean. These data, presented in Section VIII of the petition (BASF, 2011) showed no 

differences in arthropod damage or arthropod pests and beneficial insect abundance between 

BASF CV127 Soybean and other varieties, supporting the conclusion that BASF CV127 

Soybean is unlikely to impact food sources for migratory bird species. Additionally, as 

discussed in Subsection 4.5.2, Preferred Alternative – Biological Resources, BASF has 

presented evidence that the introduced proteins in BASF CV127 Soybean do not differ in 

their digestibility, toxicity and allergenicity from conventional soybeans (BASF, 2011; 

BASF, 2012 ). BASF has evaluated compositional characteristics of this soybean, including 

nutrients, anti-nutrients and other key compositional elements; no biologically meaningful 

differences were identified (BASF, 2011; BASF). Moreover, the introduced genetic material 

does not result in the production of novel proteins, enzymes, or metabolites in the plant that 

are known to have toxic properties (USDA-APHIS, 2012). BASF has also presented the 

results of a feeding study in which mice were fed very high doses of AtAHAS protein, with 

no toxic effects (BASF, 2011; BASF, 2012 ). It is thus unlikely that the migratory birds that 

may occasionally forage in soybean fields will be affected by the direct consumption of BASF 

CV127 Soybean. 

Based on these findings, it is highly unlikely that approval of the petition for nonregulated status

for BASF CV127 Soybean would impact migratory birds. 

7.2 INTERNATIONAL IMPLICATIONS

EO 12114 (US-NARA, 2010), “Environmental Effects Abroad of Major Federal Actions”

requires Federal officials to take into consideration any potential environmental effects outside 

the U.S., its territories, and possessions that result from actions being taken. 

USDA-APHIS has given this EO due consideration and does not expect a significant 

environmental impact outside the U.S. should nonregulated status be granted to BASF CV127

Soybean. USDA-APHIS assumes that the cultivation of BASF CV127 Soybean in other soybean 

production regions of the world would be conducted only after appropriate local approvals are 

received. 

It should be noted that all the existing national and international regulatory authorities and 

phytosanitary regimes that currently apply to introductions of new soybean cultivars 

internationally, apply equally to those covered by an USDA-APHIS approval of the petition for

nonregulated status under 7 CFR Part 340. 

Any international trade of BASF CV127 Soybean subsequent to approval of the petition for 

nonregulated status for the product would be fully subject to national phytosanitary requirements 

and be in accordance with phytosanitary standards developed under the International Plant 

Protection Convention (IPPC, 2010). The purpose of the IPPC “is to secure a common and 

effective action to prevent the spread and introduction of pests of plants and plant products and 

to promote appropriate measures for their control” (IPPC, 2010). The protection it affords extends 

to natural flora and plant products and includes both direct and indirect damage by pests, 

including weeds. 
Final BASF CV127 Soybean EA

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The IPPC establishes a standard for the reciprocal acceptance of phytosanitary certification 

among the nations that have signed or acceded to the Convention (177 countries as of 

October, 2012, see http://www.fao.org/fileadmin/user_upload/legal/docs/1_004s-e.pdf ). In April 

2004, a standard for pest risk analysis (PRA) of living modified organisms (LMOs) was

adopted at a meeting of the governing body of the IPPC as a supplement to an existing 

standard, International Standard for Phytosanitary Measure No. 11 (ISPM-11, Pest Risk 

Analysis for Quarantine Pests) (FAO, 2006). LMOs are defined by the FAO as organisms that 

have been modified using techniques of modern biotechnology to express one or more new or 

altered traits (FAO, 2006). In most cases, the LMO parent organism is not normally considered 

to be a plant pest but an assessment may need to be performed to determine if the genetic 

modification (i.e. gene, new gene sequence that regulates other genes, or gene product) results in 

a new trait or characteristic that may present a plant pest risk (FAO, 2006). The standard 

acknowledges that all LMOs will not present a pest risk and that a determination needs to be 

made early in the PRA for importation as to whether the LMO poses a potential pest risk 

resulting from the genetic modification. USDA-APHIS pest risk assessment procedures for 

genetically engineered organisms are consistent with the guidance developed under the IPPC.

In addition, issues that may relate to commercialization and transboundary movement of 

particular agricultural commodities produced through biotechnology are being addressed in 

other international forums and through national regulations.

The Cartagena Protocol on Biosafety is a treaty under the United Nations Convention on 

Biological Diversity (CBD) that established a framework for the safe transboundary 

movement, with respect to the environment and biodiversity, of LMOs, which includes those 

modified through biotechnology. The Protocol came into force on September 11, 2003, and 193

countries are Parties to it as of October, 2012 (CBD, 2010). Although the U.S. is not a party to 

the CBD, and thus not a party to the Cartagena Protocol on Biosafety, U.S. exporters will still 

need to comply with those regulations that importing countries which are Parties to the Protocol 

have promulgated to comply with their obligations. The first intentional transboundary

movement of LMOs intended for environmental release (field trials or commercial planting) will 

require consent from the importing country under an advanced informed agreement (AIA) 

provision, which includes a requirement for a risk assessment consistent with Annex III of the 

Protocol, and the required documentation.

LMOs imported for food, feed, or processing (FFP) are exempt from the AIA procedure, 

and are covered under Article 11 and Annex II of the Protocol. Under Article 11, Parties 

must post decisions to the Biosafety Clearinghouse database on domestic use of LMOs for 

FFP that may be subject to transboundary movement. To facilitate compliance with 

obligations to this protocol, the U.S. Government has developed a website that provides the 

status of all regulatory reviews completed for different uses of bioengineered products (see 

the NBII listings posted at http://usbiotechreg.epa.gov/usbiotechreg/. These data will be 

available to the Biosafety Clearinghouse. 

USDA-APHIS continues to work toward harmonization of biosafety and biotechnology 

consensus documents, guidelines, and regulations, including within the North American 

Plant Protection Organization (NAPPO), which includes Mexico, Canada, and the U.S., and 

within the Organization for Economic Cooperation and Development. NAPPO has 

completed three modules of the Regional Standards for Phytosanitary Measures (RSPM No. 

14), Importation and Release into the Environment of Transgenic Plants in NAPPO Member 
Final BASF CV127 Soybean EA

70

Countries (NAPPO, 2003).

USDA-APHIS also participates in the North American Biotechnology Initiative (NABI), a 

forum for information exchange and cooperation on agricultural biotechnology issues for the 

U.S., Mexico, and Canada. In addition, bilateral discussions on biotechnology regulatory 

issues are held regularly with other countries including: Argentina, Brazil, Japan, China, and 

Korea.

7.3 COMPLIANCE WITH CLEAN WATER ACT AND CLEAN AIR ACT

This Environmental Assessment evaluated the changes in soybean production due to the 

unrestricted use of BASF CV127 Soybean. This included an analysis of the potential impacts 

to water resources and air quality, presented in Subsection 4.4.2 – Physical Environment –

Preferred Alternative. Potential impacts to both resource aspects involve an attendant 

analysis of the relationships of agronomic practices and soil resources, evaluated in 

Subsections 4.4.1 Physical Environment – No Action Alternative: Soil Quality. 

As noted above, BASF has indicated that it does not intend to seek commercial cultivation 

of BASF CV127 Soybean in the U.S. Any domestic commercial cultivation will be limited 

to research and development, off-season seed production, and very limited cultivation in 

those soybean production areas where weeds resistant to the imidazolinone herbicides are not 

found. These scenarios are deemed highly unrealistic. Moreover, BASF has indicated that such 

cultivation would require a change in the US-EPA’s pesticide registration label, and have further 

asserted that BASF has no intention to seek such a label change. 

Based on these findings, it is unlikely that approval of the petition for nonregulated status for 

BASF CV127 Soybean will effect air or water quality. Based on this review, USDA-APHIS

concludes approval of the petition for nonregulated status of BASF CV127 Soybean would 

inherently comply with the Clean Water Act and the Clean Air Act. 

7.4 IMPACTS ON UNIQUE CHARACTERISTICS OF GEOGRAPHIC AREAS

As discussed in Subsection 4.2.2 – Preferred Alternative - Areas and Acreage of Soybean 

Production, cultivation of BASF CV127 Soybean will not lead to the increased production of 

soybean in U.S. agriculture. As noted above, BASF has indicated that it does not intend to 

seek commercial cultivation of BASF CV127 Soybean in the U.S. Any domestic 

commercial cultivation will be limited to research and development, off-season seed 

production, and very limited cultivation in those soybean production areas where weeds 

resistant to the imidazolinone herbicides are not found. These scenarios are deemed highly 

unrealistic. In the unlikely event that BASF CV127 Soybean is commercially cultivated, it will 

be cultivated on agricultural land currently suitable for production of soybean, and is not 

expected to increase the acreage of soybean production. BASF CV127 Soybean may displace 

currently cultivated varieties of soybean.

As discussed in Subsection 4.2.2 – Preferred Alternative - Areas and Acreage of Soybean 

Production, the common agricultural practices that would be carried out in the cultivation of 

BASF CV127 Soybean are not expected to deviate from current practices, with the exception of 

a potentially broader use of imidazolinone herbicides. Standard agricultural practices for land 

preparation, planting, irrigation, and harvesting of plants would be used on agricultural lands 
Final BASF CV127 Soybean EA

71

planted to BASF CV127 Soybean, including the use of US-EPA registered pesticides. 

Applicant’s adherence to US-EPA label use restrictions for all pesticides will mitigate potential 

impacts to the human environment. As noted above, BASF has noted that a change in the USEPA Pesticide Registration label is required in order for the imidazolinone herbicides to be used 

on BASF CV127 Soybean; an application for such a label change has not been submitted.

The Preferred Alternative evaluated in this EA is limited to approving the petition for 

nonregulated status for BASF CV127 Soybean. There are no proposed major ground 

disturbances; no new physical destruction or damage to property; no alterations of property, 

wildlife habitat, or landscapes; and no prescribed sale, lease, or transfer of ownership of any 

property. This Alternative would not convert land use to nonagricultural use and therefore would 

have no adverse impact on prime farm land.

Based on these findings, approving the petition for nonregulated status of BASF CV127 Soybean 

is not expected to impact unique characteristics of geographic areas such as park lands, prime 

farm lands, wetlands, wild and scenic areas, or ecologically critical areas. 

7.5 NATIONAL HISTORIC PRESERVATION ACT (NHPA) OF 1966 AS AMENDED 

The NHPA of 1966, and its implementing regulations (36 CFR Part 800), requires Federal 

agencies to: 1) determine whether activities they propose constitute "undertakings" that has the 

potential to cause effects on historic properties; and 2) if so, to evaluate the effects of such 

undertakings on such historic resources and consult with the Advisory Council on Historic 

Preservation (i.e., State Historic Preservation Office, Tribal Historic Preservation Officers), as 

appropriate. 

As noted above, BASF has indicated that it does not intend to seek commercial cultivation 

of BASF CV127 Soybean in the U.S. Any domestic commercial cultivation will be limited 

to research and development, off-season seed production, and very limited cultivation in 

those soybean production areas where weeds resistant to the imidazolinone herbicides are not 

found. These scenarios are deemed highly unrealistic. In the unlikely event that BASF CV127

Soybean is commercially cultivated, it will be cultivated on agricultural land currently suitable 

for production of soybean, and is not expected to increase the acreage of soybean production. 

Based on these findings, USDA-APHIS’ proposed action, approving the petition for 

nonregulated status for BASF CV127 Soybean will not adversely impact cultural resources on 

tribal properties. Any farming activities that may be taken by farmers on tribal lands are only 

conducted at the tribe’s request; thus, the tribes have control over any potential conflict with 

cultural resources on tribal properties.

USDA-APHIS’ proposed action would have no impact on districts, sites, highways, structures, or 

objects listed in or eligible for listing in the National Register of Historic Places, nor would they

likely cause any loss or destruction of significant scientific, cultural, or historical resources. This 

action is limited to approving the petition for nonregulated status for BASF CV127 Soybean.

Standard agricultural practices for land preparation, planting, irrigation, and harvesting of plants 

would be used on these agricultural lands including the use of US-EPA registered pesticides.

Applicant’s adherence to US-EPA label use restrictions for all pesticides will mitigate impacts to 

the human environment. 

USDA-APHIS’ proposed action is not an undertaking that may directly or indirectly cause 
Final BASF CV127 Soybean EA

72

alteration in the character or use of historic properties protected under the NHPA. In general, 

common agricultural activities conducted under this action do not have the potential to introduce 

visual, atmospheric, or audible elements to areas in which they are used that could result in 

effects on the character or use of historic properties. For example, there is potential for audible 

effects on the use and enjoyment of a historic property when common agricultural practices, such 

as the operation of tractors and other mechanical equipment, are conducted close to such sites. A 

built-in mitigating factor for this issue is that virtually all of the methods involved would only

have temporary effects on the audible nature of a site and can be ended at any time to restore the 

audible qualities of such sites to their original condition with no further adverse effects.

Additionally, these cultivation practices are already being conducted throughout the soybean 

production regions. The cultivation of BASF CV127 Soybean does not inherently change any of 

these agronomic practices so as to give rise to an impact under the NHPA.
Final BASF CV127 Soybean EA

73

8 LIST OF PREPARERS:

Name Organization Project Role

Michael Blanchette, B.S.

Senior Environmental 

Protection Specialist

Biotechnology

Environmental Analysis 

Branch

Reviewer/Editor

Diane Sinkowski, M.E.

Environmental Protection 

Specialist

Biotechnology

Environmental Analysis 

Branch

Reviewer/Editor

Rebecca Stankiewicz-Gabel, 

Ph.D.

Branch Chief 

Biotechnology

Environmental Analysis 

Branch

Reviewer/Editor

Charles Dobroski, M.S.

Technical Director, Ecologist

Avatar Environmental, LLC Quality Assurance and 

Biological Resources

Michael Werner, M.S., J.D.

Senior NEPA Project 

Manager, Ecologist

Avatar Environmental, LLC Project Management, Sections 

1, 2, 3, 4, 5, 6, 7

Janet Carpenter, M.S.

Agricultural Economist

Avatar Environmental, LLC Domestic and International 

Economics, Weediness, 

Herbicide Resistance, 

Agronomics

Susan Herbert, M.S.

Ecologist/Editor

Avatar Environmental, LLC References, Document 

Production, Editor, Endnotes

Florence Sevold, M.S., 

Toxicologist

Avatar Environmental, LLC Quality Assurance, Pesticide 

Exposures, Editor

Deborah Jones, M.S.

Soil Science

Avatar Environmental, LLC Quality Assurance
Final BASF CV127 Soybean EA

74

9 BIBLIOGRAPHY

Abrams, RI; Edwards, CR; and Harris, T (1978) "Yields and cross-pollination of soybeans as 

affected by honey bees and alfalfa leafcutting bees." American Bee Journal. 118 p 555-

58. 

Abud, S; de Souza, PIM; Vianna, GR; Leonardecz, E; Moreira, CT; Faleiro, FG; Júnior, JN; 

Monteiro, PMFO; Rech, EL; and Aragão, FJL (2007) "Gene flow from transgenic to 

nontransgenic soybean plants in the Cerrado region of Brazil." Genetics and Molecular 

Research. 6 (2): p 445-52. http://www.funpecrp.com.br/ >.

Abud, S; Mello de Souza, PI; Moreira, CT; Andrade, SRM; Ulbrich, AV; Vianna, GR; Rech, EL; 

and Lima Aragão, FJ (2003) "Gene flow in transgenic soybean in the Cerrado region, 

Brazil." Pesquisa Agropecuária Brasileira. 38 p 1229-35. 

Ahrent, DK and Caviness, CE (1994) "Natural cross-pollination of twelve soybean cultivars in 

Arkansas." Crop Science. 34 p 376-78. 

Aichele, TM and Penner, D (2005) "Adsorption, Desorption, and Degradation of Imidazolinones 

in Soil." Weed Technology. 19 (1): p 154-59. 

Altieri, MA (1999) "The ecological role of biodiversity in agroecosystems." Agriculture, 

Ecosystems and Environment. 74 p 19-31. 

Ammann, K (2005) "Effects of biotechnology on biodiversity: herbicide-tolerant and insectresistant GM crops." TRENDS in Biotechnology. 23 (8): p 388-94. 

Aneja, VP; Schlesinger, WH; and Erisman, JW (2009) "Effects of agriculture upon the air 

quality and climate: Research, policy, and regulations." Environmental Science &

Technology. 43 (12): p 4234–40. 

AOSCA (2009) Seed Certification Handbook: Including Genetic and Crop Standards, 

Procedures, and AOSCA Service Programs. Moline, Illinois: Association of Official 

Seed Certifying Agencies. 

AOSCA (2012) "National Soybean Variety Review Board " Association of Official Seed 

Certifying Agencies. 

http://www.aosca.org/VarietyReviewBoards/Soybean/2012SB_Sep_Report_FINAL.pdf

>.

AOSCA (No Date) "Seed Certification." Association of Official Seed Certifying Agencies. 

http://www.aosca.org/seed%20certification.htm >.

Araujo, ASF; Montiero, RTR; and Abarkeli, RB (2003) "Effect of glyphosate on the microbial 

activity of two Brazilian soils." Chemosphere. 52 p 799–804. 

Aref, S and Pike, DR (1998) "Midwest farmers' perceptions of crop pest infestation." Agronomy 

Journal. 90 (6): p 819-25. <Go to ISI>://000078678900017 >.

ASA (2010) "U.S. Fats & Oils Edible Consumption 2009." American Soybean Association. 

http://www.soystats.com/2010/Default-frames.htm >.

ASA (2012) "Soystats: Domestic Utilization - Soy Meal Production and Meal Use by 

Livestock." American Soybean Association. http://www.soystats.com/2012/Defaultframes.htm >.

ASTA (2011) "Existing U.S. seed industry production practices that address coexistence." 

American Seed Trade Association. 
Final BASF CV127 Soybean EA

75

ATTRA (2012) "Directory of Organic Seed Suppliers." National Center for Appropriate 

Technology, NCAT. https://attra.ncat.org/attrapub/organic_seed/search_results.php?Category=1&SubCategory=3&State=&Keyword=

&page=1 >.

Baier, AH (2008) "Organic Standards for Crop Production." National Center for Appropriate 

Technology. http://attra.ncat.org/attra-pub/PDF/nopstandard_crops.pdf >.

Baker, HG (1965) "Characteristics and Modes of Origin of Weeds." The Genetics of Colonizing 

Species. Academic Press. p 147-72. 

Baker, J; Southard, R; and Mitchell, J (2005) "Agricultural dust production in standard and 

conservation tillage systems in the San Joaquin Valley." Journal of Environmental 

Quality. 34 p 1260-69. 

BASF. "Lightning® Herbicide Label." Research Triangle Park, NC2008.

BASF (2011) "Petition for the Determination of Nonregulated Status for Imidazolinone-tolerant 

Soybean BPS-CV127-9, Submitted by James Ligon, BASF Plant Science, LP, Research 

Triangle Park, NC." 

BASF (2012 ) "Environmental Report for the Determination of Nonregulated Status for 

Imidazolinone-tolerant Soybean BPS-CV127-9." 

Berglund, DR and Helms, TC (2003) "Soybean Production." North Dakota State University

Extension Service. 

Blount, A; Wright, D; Sprenkel, R; Hewitt, T; and Myer, R (2009) "Forage Soybeans for 

Grazing, Hay and Silage." Florida Cooperative Extension Service, Institute of Food and 

Agricultural Sciences, University of Florida. 

http://edis.ifas.ufl.edu/pdffiles/AG/AG18400.pdf. >.

Bradford, KJ (2006) "Methods to Maintain Genetic Purity of Seed Stocks." University of 

California, Division of Agriculture and Natural Resources. 

http://ucanr.org/freepubs/docs/8189.pdf >.

Brady, SJ (2007) "Effects of Cropland Conservation Practices on Fish and Wildlife Habitat." The 

Wildlife Society. http://www.fsa.usda.gov/Internet/FSA_File/chap_1.pdf >.

Brookes, G and Barfoot, P (2004) Co-existence case study of North America: Widespread GM 

cropping with non GM and organic crops? Dorchester, England: PG Economics Ltd. 

Brower, LP; Taylor, OR; Williams, EH; Slayback, DA; Zubieta, RR; and Ramìrez, MI (2012) 

"Decline of monarch butterflies overwintering in Mexico: is the migratory phenomenon 

at risk?" Insect Conservation and Diversity. 5 p 95-100. 

Carpenter, J; Felsot, A; Goode, T; Hammig, M; Onstad, D; and Sankula, S (2002) "Comparative 

Environmental Impacts of Biotechnology-Derived and Traditional Soybean, Corn, and 

Cotton Crops." www.cast-science.org >.

Carpenter, JE (2011) "Impacts of GM crops on biodiversity." GM Crops. 2 (1): p 1-17. 

Caviness, C (1966) "Estimates of natural cross-pollination in Jackson soybeans in Arkansas." 

Crop Science. 6 p 211-12. 

CBD (2010) "The Cartegena Protocol on Biosafety." Convention on Biological Diversity. 

http://www.cbd.int/biosafety/ >.

Curtis, TP; Sloan, WT; and Scannell, JW (2002) "Estimating prokaryotic diversity and its 

limits." Proceedings of the National Academy of Science. 99 (16): p 10494-99. 

Dalley, CD; Renner, KA; and Kells, JJ (2001) "Weed Competition in Roundup Ready®Soybeans 

and Corn." Dept. of Crop and Soil Sciences, Michigan State University. 

http://www.ipm.msu.edu/cat06field/pdf/weedfactsheet.pdf >.
Final BASF CV127 Soybean EA

76

Darley, EF and Middleton, JT (1966) "Problems of Air Pollution in Plant Pathology." Annual 

Review of Phytopathology. 4 (1): p 103-18. 

http://www.annualreviews.org/doi/abs/10.1146/annurev.py.04.090166.000535 >.

Doran, JW; Sarrantonio, M; and Liebig, MA (1996) "Soil Health and Sustainability." Advances 

in Agronomy, Volume 56. San Diego: Academic Press. p 1-54. 

Durgan, BR and Gunsolus, JL (2003) "Developing Weed Management Strategies that Address 

Weed Species Shifts and Herbicide Resistant Weeds. Department of Agronomy and Plant 

Genetics University of Minnesota." 

http://appliedweeds.cfans.umn.edu/pubs/03pub01.pdf. >.

Dwyer, LM; Stewart, DW; and Balchin, D (1988) "Rooting characteristics of corn, soybeans and 

barley as a function of available water and soil physical characteristics." Can. J. Soil Sci. 

68 p 121-32. 

Egli, DB (2008) "Comparison of Corn and Soybean Yields in the United States: Historical 

Trends and Future Prospects." Agronomy Journal. 100 p S79-S88. 

EOP-OSTP (1986) "Coordinated Framework for Regulation of Biotechnology." 51 Federal 

Register 23302, June 26, 2006. p Executive Office of the President, Office of Science and 

Technology Policy. http://usbiotechreg.epa.gov/usbiotechreg/ >.

Erickson, EH (1975) "Honey bees and soybeans." American Bee Journal. 115 p 351-53. 

FAO (2006) "International Standards for Phytosanitary Measures, ISPM No. 11." Produced by

the Secretariat of the International Plant Protection Convention. 

https://www.ippc.int/file_uploaded/1146658377367_ISPM11.pdf >.

Farnham, D (2001) "Corn Planting Guide." Iowa State University University Extention. 

http://www.extension.iastate.edu/publications/pm1885.pdf >.

Fawcett, R and Towery, D (2002) "Conservation Tillage and Plant Biotechnology." 

Fehr, WR (2007) "Breeding for modified fatty acid composition in soybean." Crop Science. 47 p 

S72-S87. 

FOCUS (2008) "Pesticides in Air: Considerations for Exposure Assessment Report of the 

FOCUS Working Group on Pesticides in Air." http://focus.jrc.ec.europa.eu/ai/index.html

>.

Foreman, L and Livezey, J (2002) "Characteristics and Production Costs of U.S. Soybean 

Farms." http://www.ers.usda.gov/media/761260/sb974-4_1_.pdf >.

Franzen, D (1999) "Soybean Soil Fertility." North Dakota State University Extension. 

http://www.ag.ndsu.edu/pubs/plantsci/soilfert/sf1164w.htm >.

FSANZ (2012) "Approval Report for Food Derived from Herbicide-tolerant Soybean line 

CV127 Soybean (BPS-CV127-9), May 2012." Food Standards Australia New Zealand. 

http://www.foodstandards.gov.au/_srcfiles/A1064%20Soybean%20BPS-CV127-

9%20AppR.pdf >.

Gage, DJ (2004) "Infection and invasion of roots by symbiotic, nitrogen-fixing rhizobia during 

nodulation of temperate legumes." Microbiology and Molecular Biology Reviews. 68 (2): 

p 280-300. 

Galle, AM; Linz, GM; Homan, HJ; and Bleier, WJ (2009) "Avian Use of Harvested Crop Fields 

in North Dakota During Spring Migration." USDA National Wildlife Research Center -

Staff Publications. Paper 921. 

Garbeva, P; van Elsas, JD; and van Veen, JA (2008) "Rhizosphere microbial community and its 

response to plant species and soil history." Plant Soil. 302 p 19-32. 
Final BASF CV127 Soybean EA

77

Garbeva, P; van Veen, JA; and van Elsas, JD (2004) "Microbial diversity in soil: Selection of 

microbial populations by plant and soil type and implications for disease 

suppressiveness." Annual Review of Phytopathology. 42 (1): p 243-70. 

GINA (2013) "Livestock - #1 Customer for Soybean Meal." 

http://www.growinginagriculture.com/ >.

Givens, WA; Shaw, DR; Kruger, GR; Johnson, WG; Weller, SC; Young, BG; Wilson, RG; 

Owen, MDK; and Jordan, D (2009) "Survey of Tillage Trends Following The Adoption 

of Glyphosate-Resistant Crops." Weed Technology. 23 (1): p 150-55. 

Grantham, R and Arnold, DC (2007) "Benefical Insects." http://osufacts.okstate.edu >.

Green, JD and Martin, JR (1996) "Dealing with Perennial Broadleaf Weeds in Conservation 

Tillage Systems." 

http://www.ag.auburn.edu/auxiliary/nsdl/scasc/Proceedings/1996/Green.pdf >.

Gunsolus, JL (2002) "Herbicide Resistant Weeds." University of Minnesota Extension. 

http://www.extension.umn.edu/distribution/cropsystems/DC6077.html >.

Hager, AG and Refsell, D (2008) "Herbicide Persistence and How to Test for Residues in Soils." 

2008 Illinois Agricultural Pest Management Handbook. 

http://ipm.illinois.edu/pubs/iapmh/13chapter.pdf >.

Hahn, RR and Stachowski, PJ (2003) "Lightning® Registered for Use on Clearfield® Corn." 

Cornell University. http://css.cals.cornell.edu/cals/css/extension/cropping-uparchive/wcu_vol13no6_2003a1lightningweeds.pdf >.

Haney, RL; Senseman, SA; and Hons, FM (2002) "Effect of Roundup Ultra on microbial activity

and biomass from selected soils." Journal of Environmental Quality. 31 p 730-35. 

Harlan, JR (1975) "Our vanishing genetic resources." Science. 188 (4188): p 618-21. 

Hartman, HT and Kester, DE (1975) Plant Propagation: Principles and Practices. Englewood 

Cliffs, NJ: Prentice-Hall, Inc. 

Hartzler, RG (2010) "Reduction in common milkweed (Asclepias syriaca) occurrence in Iowa 

cropland from 1999 to 2009." Crop Protection. 29 (12): p 1542-44. 

Heap, I (2013) "Herbicide Resistant Weeds in Soybean Globally: United States - State Specific." 

http://www.weedscience.com/summary/Crop.aspx >.

Heatherly, L; Dorrance, A; Hoeft, R; Onstad, D; Orf, J; Porter, P; Spurlock, S; and Young, B 

(2009) "Sustainability of U.S. Soybean Production: Conventional, Transgenic, and 

Organic Production Systems." Council for Agricultural Science and Technology. 

http://www.cast-science.org/publications/index.cfm/ >.

Heatherly, LG; Reddy, KN; and Spurlock, SR (2005) "Weed Management in GlyphosateResistant and Non-Glyphosate-Resistant Soybean Grown Continuously and in Rotation." 

Agronomy Journal. 97 p 568-77. 

Heiniger, R (2000) "NC Corn Production Guide - Chapter 4 - Irrigation and Drought 

Management " http://www.ces.ncsu.edu/plymouth/cropsci/cornguide/Chapter4.html >.

Heiser, JW (2007) "Dissipation and carryover of imidazolinone herbicides in imidazolinoneresistant rice (Oryza sativa)." University of Missouri-Columbia. Dissertations and 

Theses. 

https://mospace.umsystem.edu/xmlui/bitstream/handle/10355/5096/research.pdf?sequenc

e=3 >.

Helsel, DG and Helsel, ZR (1993) "Irrigating Soybeans." http://extension.missouri.edu/p/G4420

>.
Final BASF CV127 Soybean EA

78

Helsel, ZR and Minor, HC (1993) "Soybean production in Missouri." University of Missouri 

Extension. http://extension.missouri.edu/explore/agguides/crops/g04410.htm >.

Higley, LG (1994) "Insect injury to soybeans." The Handbook of Soybean Insect Pests. 

Entomological Society of America. 

Hodges, T and French, V (1985) "Soyphen: Soybean growth stages modeled from temperature, 

daylength, and water availability." Agronomy Journal. 77 (3): p 500-05. 

Hoeft, RG; Nafziger, ED; Johnson, RR; and Aldrich, SR (2000) "Soybean as a crop." Modern 

Corn and Soybean Production. Champaign, Illinois: MCSP Publications. p 31-209 

HRAC (2013) "Guideline to the management of herbicide resistance." Herbicide Resistance 

Action Committee. 

http://www.hracglobal.com/Publications/ManagementofHerbicideResistance.aspx >.

Hussain, I; Olson, KR; and Ebelhar, SA (1999) "Impacts of tillage and no-till on production of 

maize and soybean on an eroded Illinois silt loam soil." Soil and Tillage. 52 p 37-49. 

Illinois Crop Improvement Association (2013) "Seed Directory 2013 Spring Planting, Illinois 

Certified Seed." http://www.ilcrop.com/images/stories/2013_spring_seed_directory.pdf

>.

IPPC (2010) "Official Web Site for the International Plant Protection Convention: International 

Phytosanitary Portal,." International Plant Protection Convention. 

https://www.ippc.int/IPP/En/default.jsp >.

ISO (2009) "Selection and use of the ISO 9000 family of standards." 

Jaycox, ER (1970a) "Ecological relationships between honey bees and soybeans: II The plant 

factors." American Bee Journal. 110 p 343-45. 

Jaycox, ER (1970b) "Ecological relationships between honey bees and soybeans: III The honeybee factors." American Bee Journal. 110 p 383-85. 

Johnson, S; Dunphy, J; and Poore, M (2007) "Soybeans as Forage for Grazing, Haying or 

Silage." North Carolina Cooperative Extension Service, North Carolina State University. 

http://www.ces.ncsu.edu/disaster/drought/Soybeans10-9-07.pdf >.

Krapu, GL; Brandt, DA; and Cox, RR (2004) "Less waste corn, more land in soybeans, and the 

switch to genetically modified crops: Trends with important implications for wildlife 

management." Wildlife Society Bulletin. 32 (1): p 127-36. 

Krausz, RF; Young, BG; Kapusta, G; and Matthews, JL (2001) "Influence of weed competition 

and herbicides on glyphosate-resistant soybean (Glycine max)." Weed Technology. 15 p 

530-34. 

Krishna, V; Zilberman, D; and Qaim, M (2009) "Transgenic Technology Adoption and On-Farm 

Varietal Diversity." International Association of Agricultural Economists (IAAE) 

Conference. http://ageconsearch.umn.edu/bitstream/51750/2/COTTON.pdf >.

KSU. (1997). ed.^eds. Soybean Production Handbook. Kansas State University, Agricultural 

Experiment Station and Cooperative Extension Service. Print.

Kuepper, G (2003) "Organic Soybean Production." National Center for Appropriate Technology. 

http://attra.ncat.org/attra-pub/PDF/organicsoy.pdf >.

Leep, R; Undersander, D; Min, D; Harrigan, T; and Grigar, J. "Steps to Successful No-till 

Establishment of Forages." Extension Bulletin E-2880. Lansing, MI: Michigan State 

University Extension, 2003. http://www.fieldcrop.msu.edu/documents/E2880.pdf.

Lovett, S; Price, P; and Lovett, J (2003) "Managing Riparian Lands in the Cotton Industry." 

Cotton Research and Development Corporation. 
Final BASF CV127 Soybean EA

79

http://live.greeningaustralia.org.au/nativevegetation/pages/pdf/Authors%20L/18_Lovett_

Price.pdf >.

MacGowan, BJ; Humberg, LA; Beasley, JC; DeVault, TL; Retamosa, MI; and Rhodes, OE, Jr. 

(2006) "Corn and Soybean Crop Depredation by Wildlife." Purdue University, 

Department of Forestry and Natural Resources Publication FNR-265-W. 

http://www.tn.gov/twra/pdfs/cornsoydamage.pdf >.

Massey, R and Ulmer, A (2010) "Agriculture and Greenhouse Gas Emissions." University of 

Missouri Extension. http://extension.missouri.edu/p/G310 >.

May, MJ and Wilson, RG (2006) "Weeds and weed control." In Sugar Beet. Oxford: Blackwell 

Publishing. p 359-86. 

McBride, WD and Greene, C (2008) "The Profitability of Organic Soybean production." 

American Agricultural Economics Association Annual Meeting. 

McCray, K (2012) "Groundwater: Out of Sight, But Not Out of Mind." National Ground Water 

Association. http://www.ngwa.org/Events-Education/awareness/Pages/Editorial.aspx >.

McKean, A to: Michael P. Blanchette, USDA-APHIS. (2013). Updated Global Regulatory

Approval Status for Imidazolinone-tolerant Soybean BPS-CV127-9. 

Mendes, R; Kruijt, M; de Bruijn, I; Dekkers, E; van der Vorrt, M; Schneider, JHM; Piceno, YM; 

DeSantis, TZ; Andersen, GL; Bakker, PAHM; and Raaijmakers, JM (2011) "Deciphering 

the rhizophere microbiome for disease-suppressive bacteria." Science. 332 p 1097-100. 

Micro Flo Company (2012) "Banvel®Label." 

Mississippi Crop Improvement Association (2008) "General Seed Certification Standards." 

http://mcia.msstate.edu/pdf/gen-seed-cert-standards.pdf >.

NAPPO (2003) "Regional Standards for Phytosanitary Measures (RSPM) 14: Importation and 

Release (into the Environment) of Transgenic Plants in NAPPO Member Countries." 

http://www.nappo.org/Standards/Std-e.html >.

N.C. State University (2012) "Organic Grain Seed Suppliers." 

http://www.organicgrains.ncsu.edu/production/seedsuppliers.htm >.

NCAT (2003) "NCAT's Organic Crops Workbook: A Guide to Sustainable and Allowed 

Practices." National Center for Appropriate Technology. http://attra.ncat.org/attrapub/PDF/cropsworkbook.pdf >.

NCGA (2012) "Corn. Rooted in Human History; 2012 World of Corn." National Corn Growers 

Association. Last Accessed: 17 July 2012 < 

http://www.ncga.com/files/pdf/2012WOC.pdf >.

NRC (2010) The Impact of Genetically Engineered Crops on Farm Sustainability in the United 

States. Washington, DC: National Academies Press. 

http://www.nap.edu/catalog.php?record_id=12804 >.

NSRL (2012) "Soybean Production Basics." National Soybean Research Laboratory. 

http://www.nsrl.illinois.edu/general/soyprod.html >.

OECD (2000) "Consensus document on the biology of Glycine max (L.) merr. (soybean). 

ENV/JM/MONO(2000)9. ." Organisation for Economic Co-operation and Development. 

Oplinger, ES and Amberson, EA (1986) "Soybean Seed Quality and Certified Seed." University 

of Wisconsin - Madison, Dept. of Agronomy. 

OSTP (2001) "Case Studies of Environmental Regulation for Biotechnology: Herbicide-Tolerant 

Soybean." The White House Office of Science and Technology Policy. 

http://www.whitehouse.gov/files/documents/ostp/Issues/ceq_ostp_study4.pdf >.
Final BASF CV127 Soybean EA

80

Owen, MDK and Zelaya, IA (2005) "Herbicide-resistant crops and weed resistance to 

herbicides." Pest Management Science. 61 (3): p 301-11. 

Palmer, WE; Bromley, PT; and Anderson, JR (2010) "Pesticides and Wildlife - Soybeans." 

http://ipm.ncsu.edu/wildlife/soybeans_wildlife.html >.

Palmer, WE; Bromley, PT; and Anderson, JR, Jr. (No Date) "Pesticides and Wildlife – Soybeans

" North Carolina Cooperative Extension Service. 

http://ipm.ncsu.edu/wildlife/soybeans_wildlife.html >.

Pedersen, P (2007) "Seed Inoculation." Iowa State University Extension. 

http://extension.agron.iastate.edu/soybean/production_seedinoc.html >.

Pioneer (2012) "Field Facts: Soybean Variety Maturity Ratings." 

https://www.pioneer.com/home/site/ca/template.CONTENT/guid.01FD7761-12B6-

F2DC-5E45-015867C14C14/ >.

Pleasants, JM and Oberhauser, KS (2012) "Milkweed loss in agricultural fields because of 

herbicide use: effect on the monarch butterfly population." 

http://www.mlmp.org/Results/Findings/Pleasants_and_Oberhauser_2012_milkweed_loss

_in_ag_fields.pdf >.

Ray, J; Kilen, T; Abel, C; and Paris, R (2003) "Soybean natural cross-pollination rates under 

field conditions." Environmental Biosafety Research. 2 p 133-38. 

Robertson, GP and Swinton, SM (2005) "Reconciling agricultural productivity and 

environmental integrity: A grand challenge for agriculture." Frontiers in Agriculture and 

the Environment. 3 p 38-46. 

Robinson, AP and Conley, SP (2007) "Soybean Production Systems: Plant Populations and 

Seeding Rates for Soybeans." Purdue University Extension. 

http://www.ces.purdue.edu/extmedia/AY/AY-217-W.pdf >.

Ronald, P and Fouche, B (2006) "Genetic Engineering and Organic Production Systems." 

University of California, Division of Agriculture and Natural Resources. 

http://anrcatalog.ucdavis.edu/pdf/8188.pdf >.

Ruhl, G (2007) "Crop Diseases in Corn, Soybean, and Wheat." Department of Botany and Plant 

Pathology, Purdue University. 

http://www.btny.purdue.edu/Extension/Pathology/CropDiseases/Corn/ >.

Ruhl, G; Whitford, F; Weller, S; and Dana, M (2008) "Diagnosing Herbicide Injury on Garden 

and Landscape Plants." www.extension.purdue.edu/extmedia/ID/ID_184_W.pdf >.

Sellers, BA; Ferrell, JA; and MacDonald, GE (2011) "Herbicide Resistant Weeds." 

http://edis.ifas.ufl.edu/ag239 >.

Senseman, SA (2007) "Herbicide Handbook, Ninth Edition (Imidazolinone Herbicide 

Overview)." Weed Science Society of America. 

Sharpe, T (2010) "Cropland Management (Chapter 4)." Tarheel Wildlife: A Guide for Managing 

Wildlife on Private Lands in North Carolina. Raleigh: North Carolina Wildlife Resources 

Commission. p 26-29. Last Accessed: November 9, 2010 < 

http://www.ncwildlife.org/tarheelwildlife/documents/Tarheel_Wildlife_ch_4.pdf >.

SOWAP (2007) "Impact of Conservation Tillage on Terrestrial Biodiversity." Soil and Water 

Protection Project. http://www.sowap.org/results/biodiversity.htm >.

Soyatech (2011) "Soy Facts." Soyatech, LLC. http://72.32.142.180/soy_facts.htm >.

SSCA (No Date) "General Seed Certification Standards." 

http://www.ag.auburn.edu/auxiliary/ssca/general_standards06.pdf >.
Final BASF CV127 Soybean EA

81

SSDW (No Date) "Soybean Disease Atlas 2nd Edition." Southern Soybean Disease Workers. 

http://cipm.ncsu.edu/ent/ssdw/soyatlas.htm >.

Stamps, WT; Dailey, TV; Gruenhagen, NM; and Linit, MJ (2008) "Soybean yield and resource 

conservation field borders." Agriculture, Ecosystems & Environment. 124 (1-2): p 142-

46. 

Standifer, LN; Moeller, FE; Kauffeld, NM; Herbert Jr., EW; and Shimanuki, H (1977) 

"Supplemental feeding of honeybee colonies." United States Department of Agriculture. 

http://www.beesource.com/resources/usda/supplemental-feeding-of-honey-bee-colonies/

>.

Stewart, CN; Halfhill, MD; and Warwick, SI (2003) "Genetic modification: Transgene 

introgression from genetically modified crops to their wild relatives." Nature Reviews 

Genetics. 4 (10): p 806-17. 

Stewart, SD; Layton, B; and Catchot, A (2007) "Common Benefical Arthropods Found in Field 

Crops." University of Tennessee Extension. 

http://msucares.com/pubs/publications/e0020.pdf >.

Sundstrom, FJ; Williams, J; Van Deynze, A; and Bradford, KJ (2002) "Identity Preservation of 

Agricultural Commodities." http://anrcatalog.ucdavis.edu/pdf/8077.pdf >.

Tan, S; Evans, RR; Dahmer, ML; Singh, BK; and Shaner, DL (2005) "Imidazolinone-tolerant 

crops: history, current status and future." Pest Management Science. 61 (3): p 246-57. 

Towery, D and Werblow, S (2010) "Facilitating Conservation Farming Practices and Enhancing 

Environmental Sustainability with Agricultural Biotechnology." 

http://www.ctic.purdue.edu/media/pdf/Biotech_Executive_Summary.pdf >.

Tu, M; Hurd, C; and Randall, JM (2001) Weed Control Methods Handbook: Tools and 

Techniques for Use in Natural Areas. Ed. Conservancy, The Nature: UC Davis, CA. 

University of Arkansas (2006) "Soil and Water Management, Soybeans – Crop Irrigation." 

http://www.aragriculture.org/soil_water/irrigation/crop/soybeans.htm >.

University of Illinois (2003) "USDA Soybean Germplasm Collection." Department of Crop 

Sciences, College of Agriculture, Consumer and Environmental Sciences, University of 

Illinois Extension. 

http://agronomyday.cropsci.illinois.edu/2003/exhibits/soybean_germplasm.html >.

US-EPA (1989) "Imazethapyr chemical fact sheet." 

US-EPA (1990) "Citizen’s Guide to Ground-water Protection " 

US-EPA (1996) "Managing NPS from Agriculture." 

http://water.epa.gov/polwaste/nps/outreach/point6.cfm >.

US-EPA (2000) "Environmental Fate and Ecological Risk Assessment for the Registration of 

Glufosinate-ammonium on Canola, Sugar Beets and Potatoes." Office of Pesticide 

Programs, Environmental Fate and Effects Division, Environmental Risk Branch 1V. 

US-EPA (2002) "Environmental implications of conservation tillage: A systems approach." Last 

Accessed: May 19, 2010 < 

http://cfpub.epa.gov/si/si_public_record_report.cfm?dirEntryId=39100&fed_org_id=770

&SIType=PR&TIMSType=&showCriteria=0&address=nerl&view=citation&keyword=P

esticides&sortBy=pubDateYear&count=100&dateBeginPublishedPresented >.

US-EPA (2003) "National Management Measures for the Control of NonPoint Pollution from 

Agriculture " Office of Water. 

US-EPA (2005a) "Level I Screening Ecological Risk Assessment for the Reregistration of 

Imazapyr." 
Final BASF CV127 Soybean EA

82

US-EPA. "Protecting Water Quality from Agricultural Runoff." Washington, DC: Nonpoint 

Source Control Branch (4503T), EPA 841-F-05-001, 2005b.

US-EPA (2005c) "Reregistration Eligibility Decision for 2,4-D." U.S. Environmental Protection 

Agency. http://www.epa.gov/oppsrrd1/REDs/24d_red.pdf >.

US-EPA (2006a) "40 CFR Part 180 Glyphosate; Pesticide Tolerance." Federal Register. 71 

(244): p 76180-85. 

US-EPA (2006b) "Registration Eligibility Decision (RED) for Imazapyr." 

US-EPA (2008a) "Agricultural management practices for water quality protection." Last 

Accessed: August 27, 2010 < 

http://www.epa.gov/owow/watershed/wacademy/acad2000/agmodule/ >.

US-EPA (2008b) "Glufosinate Summary Document Registration Review: Initial Docket " U.S. 

Environmental Protection Agency. 

http://www.regulations.gov/#!documentDetail;D=EPA-HQ-OPP-2008-0190-

0003;oldLink=false >.

US-EPA (2009a) "National water quality inventory: Report to Congress, 2004 reporting cycle." 

Last Accessed: May 19, 2010 < http://www.epa.gov/owow/305b/2004report/ >.

US-EPA (2009b) "Pesticide Spray and Dust Drift Fact Sheet." 

http://www.epa.gov/opp00001/factsheets/spraydrift.htm >.

US-EPA (2010a) "Pesticide Registration Manual: Chapter 11 – Tolerance Petitions: Import 

Tolerances." http://www.epa.gov/pesticides/bluebook/chapter11.html#import >.

US-EPA (2010b) "A Set of Scientific Issues Being Considered by the Environmental Protection 

Agency Regarding: Field Volatilization of Conventional Pesticides." U.S. Environmental 

Protection Agency, Scientific Advisory Panel. 

http://www.regulations.gov/#!documentDetail;D=EPA-HQ-OPP-2009-0687-0037 >.

US-EPA (2011a) "Inventory of U.S. Greenhouse Gas Emissions And Sinks: 1990 – 2009." U.S. 

Environmental Protection Agency. 

http://www.epa.gov/climatechange/emissions/downloads11/US-GHG-Inventory-2011-

Complete_Report.pdf >.

US-EPA (2011b) "Pesticides: Registration Review." 

http://www.epa.gov/oppsrrd1/registration_review/ >.

US-EPA (2012a) "Inventory of U.S. Greenhouse Gas Emissions and Sinks: 1990-2010." U.S. 

Environmental Protection Agency. 

http://www.epa.gov/climatechange/emissions/usinventoryreport.html >.

US-EPA (2012b) "Sole Source Aquifer Program." 

http://water.epa.gov/infrastructure/drinkingwater/sourcewater/protection/solesourceaquife

r.cfm >.

US-EPA (2013) "National Water Quality Assessment and Total Maximum Daily Load 

Database." http://www.epa.gov/waters/ir/ >.

US-FDA (1992) "Guidance to Industry for Foods Derived from New Plant Varieties." Federal 

Register. 57 (104): p 22984. 

http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocume

nts/Biotechnology/ucm096095.htm >.

US-FDA (2006) "Guidance for Industry: Recommendations for the Early Food Safety 

Evaluation of New Non-Pesticidal Proteins Produced by New Plant Varieties Intended 

for Food Use." 
Final BASF CV127 Soybean EA

83

http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocume

nts/biotechnology/ucm096156.htm >.

US-FDA (2012) "Biotechnology Consultation Note to File and Agency Response Letter BNF 

No. 114 BASF CV127 Soybean." 

http://www.fda.gov/Food/Biotechnology/Submissions/ucm296341.htm and 

http://www.fda.gov/Food/Biotechnology/Submissions/ucm296342.htm >.

US-FWS (2013) "Threatened and Endangered Species Reports - Listings and Occurrences for 

Louisiana, Alabama, Mississippi, Georgia, Florida, and South Carolina." 

http://ecos.fws.gov/tess_public/pub/stateListingAndOccurrence.jsp >.

US-NARA (2010) "Executive Orders Disposition Tables Index." United States National 

Archives and Records Administration. http://www.archives.gov/federalregister/executive-orders/disposition.html >.

USB (2009) "Food and Feed." United Soybean Board. 

http://www.soyconnection.com/soybean_oil/pdf/foodvsfuel_soy_biofuels.pdf >.

USB (2011) "U.S. Soybean Production." United Soybean Board. 

USDA-AMS (2011) "National Organic Policy Memo GMOs." USDA Agricultural Marketing 

Service, National Organic Program. 

http://www.ams.usda.gov/AMSv1.0/getfile?dDocName=STELPRDC5090396 >.

USDA-AMS (2012) "National Organic Program." Agricultural Marketing Service, United States 

Department of Agriculture. http://www.ams.usda.gov/AMSv1.0/nop >.

USDA-APHIS (2012) "Plant Pest Risk Assessment for Imidazolinone-tolerant Soybean BPSCV127-9." 

USDA-APHIS (2013) "Petitions for Nonregulated Status Granted or Pending by APHIS." 

http://www.aphis.usda.gov/biotechnology/not_reg.html >.

USDA-ARS (2011) "At ARS, the Atmosphere Is Right for Air Emissions Studies." 

http://www.ars.usda.gov/is/AR/archive/jul11/emissions0711.htM? >.

USDA-ARS "Summary Statistics of Holdings as of 06 Jan 2013." U.S. Department of 

Agriculture–Agricultural Research Services. http://www.ars-grin.gov/cgibin/npgs/html/stats/genussite.pl?Glycine >.

USDA-ERS (2005) "Agricultural Chemicals and Production Technology: Soil Management." 

http://www.ers.usda.gov/briefing/agchemicals/soilmangement.htm >.

USDA-ERS (2011) "Major Uses of Land in the United States, 2007, Economic Informatin 

Bulletin Number 89." http://www.ers.usda.gov/publications/eib-economic-informationbulletin/eib89.aspx >.

USDA-ERS (2012a) "Adoption of Genetically Engineered Crops in the U.S.: Soybeans."

http://www.ers.usda.gov/data-products/adoption-of-genetically-engineered-crops-in-theus.aspx and http://www.ers.usda.gov/data-products/adoption-of-genetically-engineeredcrops-in-the-us/recent-trends-in-ge-adoption.aspx >.

USDA-ERS (2012b) "Agricultural Resources and Environmental Indicators, 2012." 

http://www.ers.usda.gov/media/874175/eib98.pdf or 

http://www.ers.usda.gov/Publications/eib-economic-information-bulletin/eib98.aspx >.

USDA-ERS (2012c) "Conservation Policy: Compliance Provisions for Soil and Wetland 

Conservation." 

http://webarchives.cdlib.org/sw1rf5mh0k/http:/www.ers.usda.gov/Briefing/Conservation

Policy/compliance.htm >.

USDA-ERS (2012d) "Corn: Background Briefing." http://ers.usda.gov/topics/crops/corn.aspx >.
Final BASF CV127 Soybean EA

84

USDA-ERS (2012e) "Irrigation Practices in Soybeans in 2006." Agricultural Resource 

Management Survey (ARMS). http://www.ers.usda.gov/data-products/arms-farmfinancial-and-crop-productionpractices/tailored-reports.aspx >.

USDA-ERS (2012f) "Soybeans & Oil Crops: Background." 

http://www.ers.usda.gov/topics/crops/soybeans-oil-crops/background.aspx >.

USDA-ERS (2012g) "Soybeans and Oil Crops: Trade." 

http://www.ers.usda.gov/topics/crops/soybeans-oil-crops/trade.aspx >.

USDA-ERS (2013a) "ARMS Farm Financial and Crop Production Practices, Tillage." 

http://www.ers.usda.gov/data-products/arms-farm-financial-and-crop-productionpractices/tailored-reports.aspx >.

USDA-ERS (2013b) "Organic Production, Acreage, Crops and Livestock and Beans, Tables 2, 3, 

and 7." http://www.ers.usda.gov/data-products/organic-production.aspx >.

USDA-FAS (2012a) "Global Agricultural Trade System Online: U.S. Soybean Exports, 2007 -

2012." http://www.fas.usda.gov/gats/default.aspx >.

USDA-FAS (2012b) "Oilseeds: World Markets and Trade." 

http://usda01.library.cornell.edu/usda/current/oilseed-trade/oilseed-trade-12-11-2012.pdf

>.

USDA-FAS (2012c) "World Agricultural Production." USDA-FAS. 

http://www.fas.usda.gov/psdonline/circulars/production.pdf >.

USDA-FAS (2013a) "Oilseeds: World Markets and Trade FOP 13-01, January 2013." USDA 

Foreign Agricultural Service. http://usda01.library.cornell.edu/usda/fas/oilseedtrade//2010s/2013/oilseed-trade-01-11-2013.pdf >.

USDA-FAS (2013b) "U.S. Imports of Other Vegetable Oils, 2006-2012 (GATS database 

retrieval)." USDA-Foreign Agricultural Service. 

http://www.fas.usda.gov/gats/PrintBicoReport.aspx?&PT=C&MYT=F&EY=2012&EM=

11&EMName=November&RT=P&PC=0080AT&PN=Other+Vegetable+Oils&SY=2005

&IP=N >.

USDA-FS (2009) "Invasive Species." U.S. Department of Agriculture–Forest Service, Pacific 

Northwest Research Station. http://www.fs.fed.us/pnw/invasives/index.shtml >.

USDA-FSA (2012) "Environmental and Cultural Resource Compliance: Migratory Bird Treaty

Act." http://www.fsa.usda.gov/FSA/webapp?area=home&subject=ecrc&topic=waf-ma >.

USDA-NASS (2007) "Quick Stats Ad-hoc Query Tool Total Insecticides Used in Soybean, 1991 

- 2006." http://quickstats.nass.usda.gov/data/printable/78C36EDF-3B78-3D67-A2ABDE154C9F490D

http://quickstats.nass.usda.gov/results/5D437B3D-1058-3C19-9436-917F7A651F41 >.

USDA-NASS (2009) "2007 Census of Agriculture, United States, Summary and State Data,

Volume 1 - Geographic Area Series, Part 51. AC-07-A-51." 

USDA-NASS (2012a) "Acreage." USDA, National Agricultural Statistics Service, Agricultural 

Statistics Board. Last Accessed: 17 July 2012 < 

http://usda01.library.cornell.edu/usda/current/Acre/Acre-06-29-2012.pdf >.

USDA-NASS (2012b) "Certified Organic Production Survey." 10-04-2012 p 184. 

http://usda01.library.cornell.edu/usda/current/OrganicProduction/OrganicProduction-10-

04-2012.pdf >.

USDA-NASS (2012c) "Crop Production, October 11, 2012." USDA National Agricultural 

Statistics Service (NASS), Agricultural Statistics Board. 
Final BASF CV127 Soybean EA

85

USDA-NASS (2012d) "Crop Values, 2011 Summary " 

http://usda01.library.cornell.edu/usda/nass/CropValuSu//2010s/2012/CropValuSu-02-16-

2012.pdf or http://www.nass.usda.gov/Publications/Todays_Reports/reports/cpvl0212.pdf 

>.

USDA-NASS "Farms, Land in Farms, and Livestock Operations 2011 Summary." U.S. 

Department of Agriculture–National Agriculture Statistics Service. 

http://usda01.library.cornell.edu/usda/current/FarmLandIn/FarmLandIn-02-17-2012.pdf

>.

USDA-NASS "National Statistics for Soybeans." U.S. Department of Agriculture–National 

Agriculture Statistics Service. 

http://www.nass.usda.gov/Statistics_by_Subject/result.php?818D7CBF-2510-3E92-

B0F0-

89D1ACD4737F§or=CROPS&group=FIELD%20CROPS&comm=SOYBEANS >.

USDA-NASS (2012g) "Quick Stats: Soybean Production 2001-2011." 

http://quickstats.nass.usda.gov/results/20932FD5-FEDB-3823-84E0-

8F81D5178A23?pivot=short_desc and 

http://www.nass.usda.gov/Statistics_by_Subject/result.php?20932FD5-FEDB-3823-

84E0-

8F81D5178A23§or=CROPS&group=FIELD%20CROPS&comm=SOYBEANS >.

USDA-NASS (2012h) "Quick Stats: Soybeans - Acres planted." U.S. Department of Agriculture, 

National Agricultural Statistics Service. Last Accessed: December 20, 2011 < 

http://quickstats.nass.usda.gov/?long_desc__LIKE=soybean&x=15&y=9#50E59E35-

FEBB-3BD5-88F8-1CD036BCBC6D query: QuickStats, Soybean Yields, measured in 

BU/Acre >.

USDA-NASS (2013) "Commodity Costs and Returns, Soybeans: 2010-2011." 

http://www.ers.usda.gov/data-products/commodity-costs-and-returns.aspx >.

USDA-NRCS (1996) "Effects of Residue Management and No-Till on Soil Quality." U.S. 

Department of Agriculture–Natural Resources Conservation Service. 

http://soils.usda.gov/sqi/management/files/sq_atn_3.pdf >.

USDA-NRCS (1999) "Conservation Tillage Systems and Wildlife." U.S. Department of 

Agriculture–Natural Resources Conservation Service. 

http://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs143_022212.pdf >.

USDA-NRCS (2003) "Wildlife Plan for CRP, Iowa Job Sheet." U.S. Department of Agriculture–

Natural Resources Conservation Service. ftp://ftpfc.sc.egov.usda.gov/IA/news/wildlifeplan.pdf >.

USDA-NRCS (2004) "Soil Biology and Land Management." 

USDA-NRCS (2006a) "Conservation Resource Brief: Air Quality, Number 0605." National 

Resources Conservation Service. 

http://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs143_023301.pdf >.

USDA-NRCS (2006b) "Conservation Resource Brief: Soil Erosion, Number 0602." National 

Resources Conservation Service. 

http://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs143_023234.pdf >.

USDA-NRCS (2006c) "Conservation Resource Brief: Soil Quality, Number 0601." National 

Resources Conservation Service. 

http://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs143_023219.pdf >.
Final BASF CV127 Soybean EA

86

USDA-NRCS (2012) "Energy Estimator Tillage." United States Department of Agriculture, 

National Resources Conservation Service. http://ecat.sc.egov.usda.gov/ >.

USDA-OCE (2012a) "World Agricultural Supply and Demand Estimates (WASDE 512)." 

http://www.usda.gov/oce/commodity/wasde/latest.pdf >.

USDA-OCE (2012b) "World Agricultural Supply and Demand Estimates (WASDE 513)." 

UT (2011) "Beaver Creek Study Final Report: Making a Splash AE03-63." 

http://economics.ag.utk.edu/bcstudy.html >.

van de Wouw, M; van Hintum, T; Kik, C; van Treuren, R; and Visser, B (2010) "Genetic 

diversity trends in twentieth century crop cultivars: A meta analysis." Theoretical and 

Applied Genetics. 120 p 1241-52. 

Van Duyn, J (2000) "Soybean Insect Management." North Carolina Cooperative Extension 

Service and North Carolina State University. 

http://www.ces.ncsu.edu/plymouth/pubs/ent/soybeanInsectMgt2.html >.

Virginia Crop Improvement Association (No Date) "Virginia Handbook of Seed Certification 

Standards." 

http://www.virginiacrop.org/VA%20Handbook%20of%20Seed%20Certification%20Stan

dards.pdf >.

Visser, B (1998) "Effects of Biotechnology on Agro-biodiversity." 

Vogel, JR; Majewski, MS; and Capel, PD (2008) "Pesticides in rain in four agricultural 

watersheds in the United States." Journal of Environmental Quality. 37 (3): p 1101-15. 

Waldroup, P and Smith, K (No Date) "Soybean Meal Information Center Soybean Use -

Poultry." United Soybean Board. http://soymeal.org/pdf/PoultrySoybeanUse.pdf. >.

Walker, TS; Bais, HP; Grotewold, E; and Vivanco, JM (2003) "Root exudation and rhizosphere 

biology." Plant Physiology. 132 (1): p 44-51. 

http://www.ncbi.nlm.nih.gov/pubmed/12746510 >.

Wallander, S; Claassen, R; and Nickerson, C (2011) "The Ethanol Decade: An Expansion of 

U.S. Corn Production, 2000-2009." 

Webster, TM; Patterson, M; Everest, J; Ferrell, J; Brecke, B; Culpepper, AS; Prostko, EP; Green, 

JD; Martin, JR; Webster, E; Kelly, S; Griffin, J; Sanders, D; Byrd, J; Kendig, A; York, A; 

Jordan, D; Fisher, L; Medlin, C; Murray, D; Norsworthy, J; Chapin, J; Nelson, L; and 

Steckel, L (2005) "Weed survey: Southern states 2005: Broadleaf crops section (cotton, 

peanut, soybean, tobacco, and forestry)." p 517-20. Las Cruces, New Mexico. 

Webster, TM; Patterson, M; Everest, J; Smith, K; Oliver, D; Norsworthy, J; Burgos, N; Scott, B; 

Byrd, J; Fristoe, C; Ferrell, J; Brecke, B; Minogue, P; Culpepper, AS; Prostko, EP; 

Moorhead, D; Moore, JM; Green, JD; Martin, JR; Williams, B; Stephenson, D; Miller, D; 

Sanders, D; Griffin, J; Bradley, K; York, A; Jordan, D; Fisher, L; Murray, D; Marshall, 

M; Hagood, S; and Johnson, C (2009) "Weed survey  Southern states 2009: Broadleaf 

crops subsection (cotton, peanut, soybean, tobacco, and forestry)." 2009 Southern Weed 

Science Society Annual Meeting. 

Weiderholt, R and Albrecht, K (2003) "Using Soybean as Forage." University of Wisconsin 

Cooperative Extension. http://www.uwex.edu/ces/crops/uwforage/SoybeanForage.htm. >.

Whitworth, RJ; Michaud, JP; and Davis, HN (2011) "Soybean Insect Management 2011." 

Kansas State University Agricultural Experiment Station and Cooperative Extension. 

http://www.ksre.ksu.edu/library/entml2/mf743.pdf >.

Wilson, EO (1988) Biodiversity. Ed. Peter, F.M. Washington, DC: National Academy Press. 
Final BASF CV127 Soybean EA

87

WSSA (2011) "Resistance." Weed Science Society of America. 

http://www.wssa.net/Weeds/Resistance/index.htm >.

Yoshimura, Y (2011) "Wind tunnel and field assessment of pollen dispersal in soybean [Glycine 

max (L.) Merr.]." Journal of Plant Science. 124 p 109-14. 

Young, IM and Ritz, K (2000) "Tillage, habitat space and function of soil microbes." Soil &

Tillage Research. 53 p 201-12. 

Zhang, LX; Kyei-Boahen, S; Zhang, J; Zhang, MH; Freeland, TB; Watson Jr., CE; and Liu, X 

(2007) "Modifications of Optimum Adaptation Zones for Soybean Maturity Groups in

the USA." Crop Management. p 10.1094/CM-2007-0927-01-RS.