# Patent

## Title
Isolation of phosphoproteins, glycoproteins and fragments thereof

## Abstract

The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of U.S. Provisional Application No. 61/391,606, filed Oct. 9, 2010 and U.S. Provisional Application No. 61/498,510, filed Jun. 17, 2011, both of which are incorporated by reference herein.


BACKGROUND OF THE INVENTION
This invention relates to methods for the isolation of phosphoproteins and glycoproteins and fragments of proteins for the analysis of biological samples, including biological fluids, biological cultures, cell cultures, cell lysates, cell-free cultures, yeast, HeLa cells, food, blood, urine, tissue and human cerebrospinal fluid.
Protein phosphorylation is the enzymatic process performed by kinases of adding phosphate group(s) to a protein. Phosphorylation is a reversible post translational modification that is important in many different cellular pathway processes including those involving enzymatic activities. It is estimated that up to a third of the proteins contained in a cell can be phosphorylated at some point in the life cycle of any particular protein (Hubbard, M. J. et al. (1993) Trends Biochem. Sci. 5, 172-177). Phosphorylation of proteins that are contained in eukaryotic cells occurs mainly on serine, threonine, and tyrosine residues with serine being the most frequently modified amino acid. One study performed on HeLa cell phosphorylation site distribution reported the relative concentration of the phosphoserine, phosphothreonine, and phosphotyrosine sites at 86.4, 11.8, and 1.8%, respectively (Olsen, J. et al. (2006) Cell. 127, 635-648).
Substantial work has been devoted to the development and improvement of methods for selective enrichment of phosphopeptides (Trojer, L., et al. (2005) J. Chromatogr. A. 1079, 197-207; Feuerstein, I. et al. (2006) J. Am. Soc. Mass Spectrom. 17, 1203-1208; Valiant, R. M. et al. (2007) Anal. Chem. 79, 8144-8153; Feuerstein, I. et al. (2005) Proteomics. 5, 46-54) but not phosphoprotein enrichment. The isolation of low concentrations of a phosphorylated protein fragment from its non-phosphorylated counterpart and a mixture of polypeptides has been shown to be difficult. Even more difficult is separating a phosphorylated protein from a mixture containing its non-phosphorylated counterpart and other proteins. The reason for this is unknown but may be because the number of phosphorylated sites on a given protein is small.
Two common strategies are currently employed for the isolation of phosphopeptides: immobilized metal ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC). IMAC is the most widely used strategy for phosphopeptide enrichment. This technique was initially developed in 1975 and was originally used to separate His-tagged proteins (Porath, J. et al. (1975) Nature. 258, 598-599). IMAC resins containing iron and gallium are the commonly used metals for the enrichment of phosphorylated species (Bonn, G. K. et al. (1990) Chromatographia. 30, 9/10; Aprilita, N. H. et al. (2005). J. Proteome Res. 4, 2312-2319; Sykora, C. et al. (2007) Protein & Peptide Letters. 14, 489-496). The other chemical strategy, MOAC (Wolschin, F. et al. (2005) Proteomics. 5, 4389-4397) employs metal oxides such as titanium dioxide (TiO2)(Mazanek, M. et al. (2007) Nat. Protoc. 2, 1059-1069), zirconium dioxide (ZrO2) (Zhou, H. et al. (2007) Electrophoresis. 28, 2201-2215; Kweon, H. K. et al. (2006) Anal. Chem. 78, 1743-1749) or mixed TiO2/ZrO2 nanoparticles embedded in a monolithic polymer (Rainer, M. et al. (2008) Proteomics. 8, 4593-4602) to selectively retain phosphopeptides from complex biological samples. In several studies, aluminum hydroxide and aluminum oxide were shown to exhibit a high and selective attraction to phosphorylated proteins (Chang, M. F. et al. (1997) J. Pharm. Sci. Technol. 51, 25-29; Lyer, S. et al. (2003) Pharm. Dev. Technol. 8, 81-86). After capture and isolation of the phosphopeptides, analysis is often performed by mass spectrometry.
More recently, a two-step phosphopeptide enrichment method was reported in which calcium cations are used to help isolate the phosphopeptides and then IMAC was used for final purification of the precipitated peptides (Zhang, H. et al. (2007) Molec. and Cell. Prot. 6.11, 2032-2042). However, the researchers found that calcium cation did not precipitate the phosphopeptides contained in a mixture. When calcium cation was added to the mixture, no precipitate was formed. Instead, a phosphate ion solution was added to the trypsin digested sample first. Then, with the phosphate present in the digested sample, calcium cation was added to the digested sample solution to precipitate calcium phosphate. Using this procedure, the calcium phosphate precipitate also pulled down the phosphopeptides that became associated with the calcium phosphate precipitate. After this process, it was further discovered that IMAC was needed for additional purification and enrichment to remove non phosphopeptides that also became associated with the calcium phosphate precipitate. Phosphoproteins, glycoproteins and glycopeptides could not be recovered by these researchers.
Glycoproteins play an essential role in the body. For instance, in the immune system, almost all of the key molecules involved in the immune response are glycoproteins. The analysis or determination of protein glycosylation has become an important goal for biomarker studies since it has long been known that cellular glycosylation profiles change significantly during oncogenesis, the process whereby normal cells are transformed to cancer cells (Hakomori, S. (1996) Cancer Res. 56, 5309-5318; Kobata, A. (1998) Glycoconj J. 15, 323-331). Similar to phosphoprotein and protein fragment isolation and analysis, the isolation and analysis of the glycoproteome presents a significant challenge for the bioanalytical chemist. The most common approach for the enrichment of glycosylated proteins is based on isolation by lectin affinity resins (Zhao, J. et al. (2006) J. Proteome Res. 5 (7), 1792-1802). A number of different resin types have been used to capture various types of glycoproteins. After enrichment and elution, the species are digested and then usually deglycosylated by protein-N-glycanase F. Finally, the proteins and the glycosylation sites are identified, primarily by mass spectrometry (Xiong, L. et al. (2003) J. Proteome Res. 2, 618-625; Alvarez-Manilla, G. et al. (2006) J. Proteome Res. 5, 701-708; Zhang, L. et al. (2005) Anal. Chem. 77, 7225-7231; Wang, Y. et al (2006) Glycobiology. 16, 514-523).
Phosphoproteins and glycoproteins and their peptides are important for general biological research and are potentially important biomarkers that could be used for the diagnosis of disease. The ability to capture both species together or separately is desirable. There exists a need to be able to distinguish between phosphoproteins and glycoproteins. There exists a need to capture, at will, both phosphoproteins and glycoproteins together or separately and selectively.
Valuable biological information can be obtained by capturing whole phosphorylated and glycosylated proteins rather than merely capturing their fragments. When enzymatic digestion is performed on a protein mixture, the resulting mix of polypeptides is very complex, composed of fragments originating from many different proteins. Analysis of this mixture requires attempting to determine the starting protein from which each particular polypeptide originated. Capturing whole phosphoproteins (or glycoproteins) and performing top down analysis simplifies the sample and provides additional insight into the position or positions that are phosphorylated (or glycosylated) on any particular protein. Once whole functionalized proteins are captured, chromatography or gel electrophoresis can be used to separate and collect individual proteins prior to enzymatic digestion.
Therefore, there exists a need for the ability to isolate phosphoproteins and/or glycoproteins in a reproducible manner, especially when they are present at very low concentrations.
SUMMARY OF THE INVENTION
The invention provides methods for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Selective metal cations are used to precipitate phosphoproteins and/or glycoproteins from complex mixtures. Selective metal cations may be used to precipitate polypeptides containing phospho and/or glycan groups from complex mixtures. For example, lanthanum (III) is used to preferentially complex into a precipitate the phosphate group and the glyco group, particularly N-linked glyco groups, contained on proteins or fragments of proteins allowing their isolation from complex mixtures. The invention also allows the controlled release and isolation of protein and protein fragments containing glycans from the initial precipitate leaving the phosphoproteins. Proteins containing phospho or glyco groups can be digested (and analyzed) while they are contained in the precipitation pellet or after the pellet containing the proteins has been dissolved. Proteins containing glyco groups can be digested and analyzed after the proteins containing these groups have been eluted from the pellet.
The precipitated proteins may be digested with an enzyme directly on the pellets formed from the method or any of the precipitates may be dissolved and treated with an enzyme. The sample preparation method and device can be used for many different types of biological samples, including biological fluids, biological cultures, cell cultures, cell lysates, cell-free cultures, yeast, HeLa cells, fresh milk, blood, urine, tissue and human cerebrospinal fluid. The proteins are precipitated, recovered and ready for analysis by electrophoresis, chromatography, mass spectrometry, IR or UV spectrometry, ELIZA, protein array, SPR or other analytical methods or combinations of method. The sample can be ready for analysis in as little as ten minutes and may allow detection limits down to the femtomole level and lower. The method and device are valuable tools in the field of protein analysis and diagnostics.
The application of metals, particularly lanthanum for the precipitation of phosphorylated and/or glycosylated proteins opens a new field of fast selective and sensitive biomarker analysis. The method can be used to determine biological pathways and mechanisms. The method was demonstrated for complex biological samples by being able to identify proteins present in the samples in low concentrations.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1: MALDI-TOF spectra showing the isolation method for phosphoproteins.
FIG. 2: MALDI-TOF spectra showing the isolation method for phosphoproteins in milk.
FIG. 3: MALDI-TOF spectra of tryptic digested HeLa cell lysate spiked with α-casein (1:100) before precipitation and after isolation.
FIG. 4: MALDI-TOF spectra showing the precipitation and recovery of a phosphoprotein and glycoprotein.
FIG. 5: MALDI-TOF spectra showing the precipitation, separation and recovery of a glycoprotein and a phosphoprotein.



DETAILED DESCRIPTION OF THE INVENTION
Before describing the present invention in detail, it is to be understood that this invention is not limited to specific embodiments described herein. It is also to be understood that the terminology used herein for the purpose of describing particular embodiments is not intended to be limiting. As used in this specification and the appended claims, the singular forms “a”, “an” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to polymer bearing a protected carbonyl would include a polymer bearing two or more protected carbonyls, and the like.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, specific examples of appropriate materials and methods are described herein. The invention provides methods and devices for the rapid and controllable precipitation of phosphoproteins, glycoproteins and their fragments. Phosphoproteins are proteins to which phosphate groups are covalently bound. A common example of a phosphoprotein is the protein casein found in milk. A glycoprotein is a molecule composed of a protein that contains oligosaccharide chains (glycans) covalently attached to polypeptide side-chains. A glycopeptide is similar in structure to a glycoprotein but consists of a chain of amino acids.
In the present invention, metal cations are used to precipitate and complex the proteins in a sample containing either phosphate groups or glycans. These proteins can be analyzed together, or through further chemical manipulation, the pellet proteins containing the phosphate groups and/or the glyco groups can be isolated and analyzed. Rare earth metals including Lanthanum (III) cations are preferred.
The characteristics of the method and device of this invention can be illustrated and performed in simple samples containing a protein mixture or in complex samples such as a biological matrix of cerebrospinal fluid. The sample preparation method and device can be used for any type of biological sample, including but not limited to biological fluids, biological cultures, cell cultures, cell lysates, cell-free cultures, yeast, HeLa cells, fresh milk, blood, urine, tissue and human cerebrospinal fluid. The recovered proteins can be analyzed by mass spectrometry or other analytical methods with detection limits at the femtomole level and lower. The method and devices are valuable tools in the field of protein analysis, biological research and diagnostics.
For the isolation procedure of the invention, several metals can be used. The metal is added to the sample to precipitate proteins. The sample may be centrifuged at this point to recover the precipitate containing the phosphoproteins and/or glycoproteins. After precipitation, optionally additional phosphate can be added to produce more precipitate, after the metal cation has been added to the sample first and the first precipitate has been formed. It is remarkable that a metal can selectively bind with phophosproteins and/or glycoproteins to form a precipitate. It is not known, but presumably the precipitate structure would contain many protein molecules associated with each other in such a way that the proteins are insoluble. It is unknown how the proteins form the precipitate with the addition of the metal ion.
Previous workers discovered that no precipitate is formed when calcium is added to a biological sample containing phosphopeptides (Zhang, H., et al. (2007) Molec. and Cell. Prot. 6.11, 2032-2042). Polypeptides are much smaller than proteins. However surprisingly and unexpectedly in this invention, metals such as silver, lead, strontium, barium, copper, zinc or others metal can be used to form a precipitate with phosphoproteins and glycoproteins when the metal is added to a sample. Proteins are very large molecules and the portion of the protein molecule containing the phospho groups or glyco groups can be a relatively small portion of the molecule. The selective precipitation of either phosphoproteins or glycoproteins from a sample is unexpected. Trivalent and higher valent metals are particularly effective and include antimony, iron, gold, rare earth metals and others. Rare earth metals are preferred and include lanthanum, terbium, europium, erbium and others. Lanthanum is preferred because it is inexpensive, forms precipitate with both phosphoproteins and glycoproteins, forms protein precipitate at low concentrations of the metal ion and is relatively non-toxic. In one embodiment of the invention, LaCl3 solution is added to a biological sample.
Different additives can be added to the solution to enhance formation of the precipitate. These include salts, organic solvents and water soluble polymers. A non-limiting list of water miscible organic solvents that could be added includes alcohols, ketones, aldehydes and other organics or mixtures thereof. Water soluble polymers including polyelectrolytes and non ionic polymers or mixtures may be added. Salts may be added to reduce the solubility of the precipitate. Salts may be added to form additional precipitates with any free or left over metal ions that have not precipitated with the proteins.
The temperature of the solution can be varied. For example, the kinetic rate of precipitation can be increased by increasing the solution temperature. The amount of precipitate formed can be increased by decreasing the solution temperature.
Other metals will form phosphoprotein and/or glycoprotein precipitation complexes including for example, lanthanum, cadmium, thallium, silver and barium and mixtures of these. Lanthanum metal is preferred because it is nontoxic, inexpensive and effective. The solubility of the lanthanum phosphoproteins and glycoproteins precipitate complexes was found to be low. Rare earth metal salts of terbium, europium and erbium are very effective in forming precipitates with phosphoproteins.
In one embodiment, the biological sample is not denatured. However, denaturing the sample prior to addition of the metal may be used to improve the capture, digestion and analysis. In certain embodiments, the biological sample may be denatured to enhance or improve the ability of the Lanthanum or other metals to precipitate the proteins. Any method for denaturing the proteins within a biological sample can be used. For example, the sample can be treated with heat, acid, base, alcohol, reducing agents, or heavy metal salts such as Hg+2, Pb+2, Ag+1Tl+1, Cd+2 and other metals with high atomic weights. A common denaturing agent is a mixture of dithiotreitol (DTT), 1-O-n-oxtyl-β-D-glucopyranoside (nOGP) and iodoacetamide (IAA). This mixture helps denature the sample by breaking up the disulfide bonds by DTT and alkylating the thio groups with IAA. The nOGP is a nonionic detergent used with proteins. The term, “denature biological sample”, as used herein can include biological samples in which the proteins are partially denatured or samples in which a portion of the proteins are denatured. Denaturing the proteins can be performed at different steps in the process. When denaturing is performed prior to capture, the protein is denatured to help capture and precipitate the entire protein. In other examples, the protein may be denatured at a later stage for example, to improve digestion with an enzyme.
To demonstrate the value of denaturing the sample before precipitation, the following experiment was carried out. Two identical (1 mL) urine samples were analyzed. The first urine sample was prepared without denaturing. Lanthanum metal ion was added to the sample and a precipitate was formed and recovered. The second urine sample was denatured by the addition of 20 μL DTT (45 mM) and 20 μL of nOGP (40 mM) followed by vortex mixing at 95° C. for 5 min. After cooling down the sample to room temperature, 20 μL of IAA (100 mM) were added for the alkylation of the thiol groups. Afterwards the sample was stored in the dark for 30 min. Lanthanum metal was added to the denatured sample and the precipitate was recovered into a pellet. The two pellets were digested with an enzyme and the supernatant digestion fraction was recovered for both samples. The digested fraction was analyzed by nanoLC-ESI-MS. The analysis conditions were as follows: PepMap LC Packings column, 50 cm×75 μm with a particle size of 3 μm and 120 Å pore size. The flow rate was 300 mL/min and the gradient from 100% A to 60% A in 120 min (A: H2O, 3% ACN, 0.1% formic acid; B: 80% ACN, 20% H2O, 0.1% formic acid).
The number of identified proteins after the two runs was compared. Without denaturing the sample, 101 proteins were identified (72 phosphorylated and 29 glycosylated). In the experiment performed by first denaturing the sample, 426 proteins were identified (288 phosphorylated and 138 glycosylated) showing the positive influence of the denaturing step. A denaturing step prior to precipitation is preferred for enhanced recovery of both phosphoproteins and glycoproteins.
Lanthanum (III) cations added to the sample solution will begin to precipitate proteins and/or fragments thereof that contain phospho groups and/or glyco functional groups. The mechanism for precipitating phosphate containing proteins containing proteins with a metal is unknown. For a precipitate to form, many molecules must be linked together to form a very large lanthanum phosphate containing insoluble molecular structure. It is unexpected and surprising that a metal cation phosphoprotein precipitate can be formed and recovered since it was reported earlier that calcium does not form a precipitate with phosphopeptides, which are much smaller molecules (Zhang, H., et al. (2007) Molec. and Cell. Prot. 6.11, 2032-2042).
The forces and structure of a precipitate of lanthanum and a glycan protein are even less clear and it is unexpected and surprising that a metal cation phosphoprotein precipitate can be formed and recovered. It is possible that one or more Lanthanum cations may form a structure with exposed hydroxide groups on the glycoprotein, but it is unknown how these metal protein structures are configured and why they are strong enough and structured in way to be removed by precipitation. Lanthanum (III) has a preference for N-linked glycoproteins over other glycoproteins. Other metals will complex O-linked, C-linked, phospho linked and other glycans.
The precipitate solid may remain in suspension in the sample or may fall out of solution. In one embodiment, the precipitate formed from the addition of metal ion or lanthanum (III) to the biological sample may be centrifuged and processed to recover and analyze the phosphoproteins and glycoproteins or protein fragments in the sample. In another embodiment, additional phosphate in the form of K2HPO4 or another form of phosphate may be added to the solution. The addition of this phosphate does not have an effect on the initial metal protein precipitates formed. However, additional metal phosphate precipitate may be formed to increase the total precipitate mass. In some embodiments the phosphate may be added before the metal ion is added. The increase in precipitate mass is formed from the excess unused (uncomplexed or unprecipitated) metal that may be present. In some embodiments, other anions may be added to the mixture to enhance the precipitate being formed. For example, carbonate, hydroxide and iodate can be used.
In another embodiment, the precipitated sample may be treated to selectively recover only the phosphoproteins or phosphoprotein fragments. A phosphate buffer such as K2HPO4 may be added after precipitation of the sample. In a previous embodiment, it was described that the addition of phosphate anion after the initial addition of the metal cation will form a precipitate with the excess left over metal cation (uncomplexed metal cation). It was discovered that the addition of phosphate can have a positive effect because more precipitate mass is formed and it takes less centrifugation time to form the pellet. This procedure of the second addition of phosphate ion is different because no additional precipitate is formed. It was discovered unexpectedly and surprisingly, that if the phosphate anion is applied at this time at sufficient concentration and without the excess metal present, it will “break up” or “displace” the metal glycoprotein or protein fragment from the precipitate complex and the glycoproteins will be found in the liquid of the mixture. Other glycogen solubilizing reagents may be used to remove the glycoproteins. These include boric acid, borate anion, carbonate, hydroxide, oxalate or anion material that will complex or displace glycoproteins from the pellet.
In some embodiments, after the addition of phosphate anion, the glycoproteins will re-dissolve and remain in the supernatant along with the other non phosphoproteins and other biological material in the initial centrifugation. In certain embodiments, the pellet is centrifuged, washed and supernatant discarded. In this embodiment, the glycoproteins will re-dissolve into the supernatant. Thus, the process carried out on the precipitation pellet from this point will result in the recovery and analysis of only phosphoproteins and protein fragments and not glycoproteins and protein fragments. However in some embodiments, the dissolved portion of the sample, the supernatant, can be taken to recover and analyze glycoproteins and glycopeptides.
The precipitate of metal phosphoproteins and metal glycoproteins formed in the initial precipitation reaction may be centrifuged or filtered and recovered. After the metal protein precipitate is formed, the solution containing the metal protein precipitate and unused metal ion may be treated with a phosphate buffer such as K2HPO4 solution to increase amount of precipitate obtained. Although not normally desired, it is possible that if enough phosphate anion is added at this point or later, the phosphate ion may dissolve or breakup the glycoprotein complex and allow recovery the glycoproteins alone in solution leaving the phosphoproteins intact in the precipitate and the glycoproteins in the supernatant. At this point, either class of proteins could be processed and analyzed directly as whole proteins, with CE, HPLC, or MALDI for example, or digested with an enzyme and analyzed as polypeptides with HPLC and or mass spectrometry for example.
After the pellet is formed, it washed to remove materials not of interest. Water or different buffers or solutions can be used. A buffer containing K2HPO4 can be used to inhibit the binding acidic peptides and proteins to lanthanum and wash these materials from the pellet. Several amino acids are acidic and may lower the pl of a polypeptide or proteins. Body fluid samples may contain may polypeptides. Polypeptides may be formed by enzymatic digestion. Peptides with Asn or Asp amino acid residues may coordinate La3+ and coprecipitate and may need to be washed from the pellet. In some embodiments, after precipitate pellet is formed, the precipitate can be treated with a denaturing solvent such as urea and denaturing surfactant such as CHAPS. The functionalized proteins or protein fragments that are bound to the precipitate remain bound or part of the precipitate but will denature in order to get rid of unspecific proteins associated with the protein interaction by non-covalent interactions. It should be noted that the description “proteins remain bound” is used here to describe proteins within the pellet or precipitate, but the mechanism and form by which the functionalized proteins are associated with the precipitate are unknown. The denaturing treatment may be performed with the precipitate in suspension. The pellet with bound, denatured phosphoproteins and/or glycoproteins or protein fragments can be reformed by centrifugation and then the pellet can be washed by repeated re-suspension and centrifugation in deionized water or buffer. In one embodiment, one denaturing step is used and four washing steps are used but any number of denaturing and washing steps may be used.
In one embodiment, after final washing, centrifugation and removal of supernatant, the bound functionalized proteins are recovered by optionally re-suspending and then dissolving the pellet into solution by lowering the solvent pH. Then the solution containing the functionalized protein or protein fragments may be analyzed by mass spectrometry, gel electrophoresis, 2D-gel electrophoresis or any analytical method including chromatography, capillary electrophoresis, etc.
In one embodiment, the digestion of the proteins with an enzyme may be performed after re-dissolving the pellet. In another embodiment, the whole proteins or protein fragment bound to the precipitate may be digested on pellet with an enzyme. Then peptides released by the digestion may be analyzed by LC-MS or other techniques to determine the peptide mass fingerprint and location of the functional groups.
DEFINITIONS
The terms “protein” “proteins” and “protein fragments” and “fragments of proteins” are used as equivalent terms herein.
The terms “protein fragment”, “fragments of protein”, “peptides” and “polypeptides” are equivalent terms in describing a part or portion of a protein.
The terms “bound”, “precipitate”, “complex”, “precipitate complex” and “solid phase” are equivalent terms in describing the precipitate.
The terms “phosphoproteins” and “glycoproteins” also include fragments thereof in the method of the invention.
The terms “glycoproteins” and “glycosylated proteins” are considered equivalent terms herein.
The terms “phosphoproteins” and “phosphorylated proteins” are considered equivalent terms used in the method of the invention.
The terms phosphoprotein or protein having a phospho functional group are considered equivalent terms used in the method of the invention.
The terms glycoprotein, glycan or protein having a glyco functional group are considered equivalent terms used in the method of the invention.
The terms functionalized protein (or polypeptide) as used herein refers to proteins or polypeptides comprised of phospho or glyco groups.
The following abbreviations are used herein. (nOGP) n-Octyl-β-D-glucopyranosid, (IAA) iodoacetamide, (CSF) cerebrospinal fluid, (AD) Alzheimer's disease, (FA) formic acid, (SA) sinapinic acid, (DHB) 2,5-dihydroxybenzoic acid, (PMF) peptide finger print, (PTM) posttranslational modification and (ApoE) apolipoprotein E.
The following abbreviations are used herein. (nOGP) n-Octyl-β-D-glucopyranosid, (IAA) iodoacetamide, (CSF) cerebrospinal fluid, (AD) Alzheimer's disease, (FA) formic acid, (SA) sinapinic acid, (DHB) 2,5-dihydroxybenzoic acid, (PMF) peptide finger print, (PTM) posttranslational modification and (ApoE) apolipoprotein E.







TABLE 1







Method for the isolation of phosphorylated and glycosylated proteins by Lanthanum


(III) precipitation. Other metals may be substituted for this method. Comments


and some of the possible variations on the method are included in the table.













Amount,




Step
Description
volume, etc.
Function
Comment





1
Provide
Typically 10-

Can be from cancer cells or



phosphorylated
1000 μL of

human cerebrospinal fluid,



protein and/or
sample

etc. The sample may be



glycoprotein sample


whole protein or be






digested to provide






polypeptides or fragments






of proteins. Samples






comprised of whole






proteins are preferred. The






sample may be denatured






to facilitate precipitation.






Smaller and larger sample






sizes are possible with






proper adjustment of






reagents. In some






embodiments the sample






pH may be adjusted before






step 2 in order to reduce






interaction of the metal ion






with acetic groups on the






proteins.


2
Add Lanthanum (III),
1.5-7 μL of 1M
Forms insoluble salt
Cloudy solution is formed



mix sample by vortex
LaCl3, larger
complex with
with some particulate




volumes are
phosphorylated proteins
falling to the bottom of vial.




used for larger
Forms insoluble complex
Other metals such as rare




sample
with glycosylated proteins.
earth metals and other




volumes.

metals will also form salt






and complex insoluble






precipitates. Lanthanum






cation is preferred.






Lanthanum protein






precipitate can be






centrifuged at this point.


Optional 3
Add K2HPO4, mix
1-5 μL of 2M
The phosphate anion forms
Although not preferred, in



sample by vortex
K2HPO4, larger
La(PO4) ppt with left over
some embodiments the




volumes are
or unused metal cation
addition of phosphate ion




used for larger
(any La that did not bind to
can be performed before




sample
a protein).
step 2. The precipitates of




volumes

La-protein and La(PO4) can






be centrifuged at this point.


4
Centrifuge to form



pellet


Optional 5
Re-suspend and wash
500 μL of 8M
Denatures precipitated
Also, removes any non-



the pellet once with
urea/1%
protein in pellet
specific adsorbed protein.



urea and CHAPS in
CHAPS in PBS

Surfactant may also be



PBS buffer and then
pH 7.4

added earlier with the



centrifuge again


addition of Lanthanum.


6
Re-suspend and wash
De-ionized
Removes excess urea and
Different buffers may be



the pellet 3 times with
water
surfactant and non-specific
used to wash away



DI water, centrifuging

adsorbed protein
materials not of interest.



after each wash


Optional 7
Treat pellet with
10-300 μL of
Breaks up and dissolves
The pellet may be treated



phosphate ion
4M K2HPO4
any La-glycoprotein
with phosphate prior to or




and de-ionized
precipitate.
following separation by




water
Phosphoproteins remain in
centrifugation or a filtration





the pellet. Glycoproteins
method. No extra co-ppt





are in the supernatant and
was formed because excess





are optionally recovered
La (III) has been removed.





and analyzed by top down
The amount of phosphate





or bottom analysis.
anion added can be






adjusted to remove all of






the glycoproteins. Gel






electrophoresis and other






techniques can be used to






measure the effectiveness






of the reagent.


Optional 8
Re-suspend and wash
De-ionized



the pellet 3 times with
water (500 μL)



DI water, centrifuging



after each wash


Optional
On pellet microwave -
Several steps
Releases fragments of
Bottom up sample prep.


9
assisted trypsin

proteins through digestion
This sample preparation is



digestion


preferred.


Optional
uLC-ESI-MS analysis or


Bottom up analysis. Protein


10(follows
other method on


Identification of proteins by


optional
supernatant


MS/MS is preferred.


9)


Optional
Re-dissolve pellet
10 μL of 2.5%
Releases the proteins
Top down sample prep


11

H3PO4


Optional
CE, MALDI or other


Top down analysis


12(follows
method analysis


optional


11)









In this example, only a slight (20%) molar excess of a lanthanum (III) compound is needed to achieve complete precipitation although in practice the metal is used in greater molar excess ensuring complete capture of unknown concentrations of proteins. Sample precipitation pellets in step 2 may be formed from a progression of metal concentrations. The pellets can be analyzed by gel electrophoresis (or other protein analysis methods) to determine whether sufficient and optimum amount of metal has been used to capture proteins. The metal amount can be increased until no new proteins are captured. The precipitation process of proteins is unknown. A protein is large and bulky but presumably the functional group on the protein such as a phosphate group or a glyco group would take up only a very small portion of the protein molecule. The nature of the metal protein precipitate is unknown, but in order for a particular protein type to precipitate it must form a large, presumably multiprotein metal complex perhaps linking multiple protein molecules and multiple metal molecules. It is unknown how a multiprotein linked complex could be formed.
The sample pellets and supernatants obtained from a progression of phosphate concentrations (Table 1, optional step 7) can be analyzed by gel electrophoresis (or other protein analysis methods) to determine whether a sufficient or optimum amount of phosphate has been used to remove and capture glycoproteins. The phosphate amount can be increased until no additional protein is removed from the pellet or no new protein is recovered in the supernatant.
Some metals including europium, terbium and erbium are less effective or will not form glycoprotein precipitates. However, europium, terbium and erbium metals work well for the precipitation and recovery of phosphoproteins.
The precipitation method of the invention may be performed with centrifuges or filtration. The metal precipitating and complexing ion may be added as a liquid or be contained on an ion exchanger or other solid.
The procedure outlined in Table 2 is similar to the procedure in Table 1 except that in this example the amounts of reagent are shown for a 50 μL sample. Also in this example, the sample is denatured prior to precipitation to facilitate (selective) precipitation recovery of the phosphoproteins and glycoproteins. Any method can be used to denature the proteins. The examples cited here are only for illustrative purposes.







TABLE 2







Method for the isolation of phosphorylated and glycosylated proteins by Lanthanum (III) precipitation.













Amount, volume,




Step
Description
etc.
Function
Comment





1
Provide
50 μL of sample

Can be from cancer cells or



phosphorylated


human cerebrospinal fluid,



protein and/or


etc. The sample may be



glycoprotein sample


whole protein or be digested






to provide polypeptides or






fragments of proteins. In






some embodiments the






sample pH may be adjusted






before step 2 in order to






reduce interaction of the






metal ion with acetic groups






on the proteins.


Optional
Protein
Add 8 μL 10 mM
Ensures that all
DTT: Dithiotreitol


2
denaturation
DTT and 10 μL 45
phospho-sites and
nOGP: 1-O-n-octyl-β-D-




mM nOGP to the
glyco-sites are
glucopyranoside




sample
accessible


Optional
Further
Incubate the
Breaks up of disulfide


3
denaturation
sample for 5 min
bonds by DTT




at 95° C.


Optional
Protein alkylation
Add 10 μL of 50
Alkylation of the thiol-
IAA: Iodoacetamide.


4

mM IAA and
groups
Alkylation ensures that




incubate 30 min in

disulfides are not




the dark

reformed.


5
Add Lanthanum (III),
1.5 μL of 1M LaCl3,
Forms insoluble salt
Cloudy solution is formed



mix sample by vortex

complex with
with some particulate falling





phosphorylated proteins
to the bottom of vial. Other





Forms insoluble chelate
metals such as rare earth





complex with
metals and other metals will





glycosylated proteins.
also form salt and complex






insoluble precipitates.






Lanthanum cation is






preferred. Lanthanum






protein precipitate can be






centrifuged at this point.


Optional 6
Add K2HPO4, mix
1.5 μL of 2M K2HPO4
The phosphate anion
Although not preferred, in



sample by vortex

forms La(PO4) ppt with
some embodiments the





left over or unused metal
addition of phosphate ion





cation (any La that did
can be performed before





not bind to a protein).
step 5. The precipitates of La-






protein and La(PO4) can be






easily centrifuged at this






point.


7
Centrifuge to form



pellet


Optional 8
Re-suspend and wash
300 μL of 8M urea/
Denatures precipitated
Also, removes any non-



the pellet once with
1% CHAPS in PBS pH
protein in pellet
specific adsorbed protein.



urea and CHAPS in
7.4

Surfactant may also be



PBS buffer and then


added earlier with the



centrifuge again


addition of Lanthanum.


9
Re-suspend and wash
De-ionized water
Removes excess urea and



the pellet 3 times

surfactant and non



with DI water,

specific adsorbed protein



centrifuging after



each wash


Optional
Treat pellet with
50 μL of 4M K2HPO4
Breaks up any La-
The pellet may be treated


10
phosphate ion and
and de-ionized
glycosylate protein
with centrifugation or



optionally recover
water
precipitate that was
filtration. No extra co-ppt



supernatant

formed in Step 2.
with phosphorylated





Phosphoproteins remain
proteins is formed because





in pellet. Glycoproteins
excess La metal cation has





are in supernatant and
been removed.





are optionally recovered





and analyzed by top





down or bottom up





analysis.


Optional
On pellet microwave-
Several steps
Releases fragments of
Bottom up sample prep


11
assisted trypsin

proteins through



digestion

digestion


Optional
μLC-ESI-MS analysis


Bottom up analysis


12 (follows
or other method on


optional
supernatant


11)


Optional
Re-dissolve pellet
10 μL of 10% TFA
Releases the proteins
Top down sample prep


13


Optional
CE, MALDI or other


Top down analysis


14 (follows
method analysis


optional


13)









The selectivity of La (III) was tested for the precipitation of a phosphoprotein out of a protein mixture. For this experiment, phosphoprotein α-casein (0.01 μg/ml) was added to a mixture of three non phosphoproteins: myoglobin (1 μg/ml), lysozyme (1 μg/ml) and bovine serum albumin (BSA) (1 μg/ml). Albumin is known to be a “sticky protein” prone to binding other proteins. Nonspecific protein-protein interactions, if present, would decrease the selectivity and reliability of the method by interacting and co-precipitating non-specific proteins together with the target phosphoproteins. To minimize these possible protein-protein interactions, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPS) zwitterion detergent was added to the sample as described in Tables 1 and 2.
The MALDI-TOF spectra (sinapinic acid matrix solution, 50% ACN, 0.1% TFA) results shown in FIG. 1 indicate the selectivity and efficiency of the precipitation method. Spectrum (A) shows the mixture before precipitation, (B) shows the spectrum of the supernatant after precipitation, and (C) shows the spectrum the precipitated pellet after it was denatured, washed and re-dissolved. Spectrum (B) shows that no signal was found for the phosphoprotein α-casein in the supernatant after the precipitation process, indicating the protein was completely precipitated as lanthanum-phosphoprotein complex. The spectrum shows that all the other non-phosphorylated proteins remained in the supernatant and MALDI-TOF peaks were still detected. Spectrum (C) showed that after dissolving the pellet, the α-casein phosphoprotein was recovered and detected with the same intensity.
The use of the reagent K2HPO4 was investigated to determine if it was possible to control the coprecipitation of glycoproteins with lanthanum cation while still precipitating phosphoproteins. In this scenario, the lanthanum would be added to the sample forming the phosphoprotein precipitate and the glycoprotein precipitate. Optionally, phosphate could be added at this point to form additional precipitate with any unused (unprecipitated or uncomplexed) metal ion. Although not preferred, the phosphate ions can be added before the metal ions in alternate embodiments.
After washing the precipitate, phosphate anions can be added to the mixture to break up or redissolve the lanthanum glycoprotein precipitate while not disturbing the lanthanum phosphoprotein precipitate. This procedure may be used because only the stronger or more likely forming lanthanum phosphoprotein and lanthanum phosphate precipitate would be recovered while the lanthanum glycoprotein precipitate complexes would break up and redissolve. This provides a way to distinguish between phosphoproteins and certain glycoproteins. If excess phosphate ions are present in the sample, these phosphate ions might sequester the lanthanum and prevent or otherwise interfere with the desirable lanthanum phosphoprotein precipitation. It is surprising that the phosphate group contained on a protein forms a stable precipitate with lanthanum cation in the presence of phosphate anion. Furthermore, it is surprising that phosphate ions or other anions could facilitate the breaking up of a glycoprotein complex allowing the glycoprotein to re-dissolve when a lanthanum glycoprotein complex precipitate has already been formed. Precipitation reactions by nature may have very slow kinetic rate constants associated with the formation reaction and therefore precipitation and re-disolving may slow considerably or may appear to stop as the reagent concentrations are decreased. It is surprising and unexpected that metal precipitation can be used to recover phosphoproteins and glycoproteins.
In order to study these selective precipitations and the related process of phosphoprotein isolation in the presence of glycoproteins, fresh milk was chosen as sample. This sample contained the phosphoproteins αs1-casein, αs2-casein and β-casein and the glycosylated protein α-lactalbumin. The sample also contained the protein β-lactalbumin which does not contain either phosphorylated or glycosylated sites. The results are shown in FIG. 2. Spectrum (A) shows the MALDI-TOF spectrum of fresh milk before lanthanum precipitation (A). The labeled peaks are (1) [M+2H]2+ caseins, (2) [M+H]+ α-lactalbumin, (3) [M+H]+ β-lactoglobulin and (4) [M+H]+ αS1-casein, (5) [M+H]+ β-casein and (6) [M+H]+ αS2-casein. The mass spectrum of the supernatant after lanthanum precipitation spectrum (B) show no caseins (phosphoproteins) can be detected in the supernatant. However, the mass of glycoprotein α-lactalbumin was still detected with the same intensity showing the glycoprotein was not precipitated. The spectrum shows that β-lactoglobulin was also not precipitated. Spectrum (C) shows the recovered precipitated pellet shows the singly and doubly charged ion signal of caseins after denaturing, washing and re-dissolving the pellet.
In a further step, the precipitated caseins were digested with trypsin directly on the pellet and measured by MALDI-TOF using DHB in 50% ACN, 0.1% TFA (puriss. p.a. Sigma-Aldrich, St. Louis, Mo., USA) as matrix. The mass peak list was submitted to the mascot search engine for peptide mass fingerprint (PMF) analysis and revealed 64% sequence coverage for αs1-casein (15 masses identified), 51% for αs2-casein (7 masses identified), and 20% for β-casein (6 masses identified). The recognized masses enabled a fast qualitative identification of the three casein proteins present in fresh milk due to the PMF.
The on-pellet digestion of precipitated lanthanum-protein complex was microwave-assisted. Several experiments were carried out for different energy levels and time periods. The best results were obtained for two minutes digestion at 70 W. At the same time, the un-precipitated protein mixture containing all four proteins was digested in-solution using the same protocol. The MALDI-TOF peak lists of both digests were submitted to the Mascot search engine for PMF analysis. No α-casein could be significantly found in the digested un-precipitated protein mixture. This was probably due to suppression and interference effects from many other signals given off by proteins that are 100 times more highly concentrated in the sample. However, a Mascot search for the on-pellet digested lanthanum-protein complex yielded total sequence coverage of 92%.
In another experiment, a dephosphorylated HeLa cell lysate spiked with α-casein (1:100) was analyzed with the developed strategy. FIG. 3 (top spectrum) depicts the MALDI-TOF (2,5-dihydroxybenzoic acid matrix) spectrum of a trypsin-digested HeLa cell lysate spiked with α-casein that has not undergone the lanthanum precipitation sample process. The α-casein could not be significantly detected in the un-precipitated digested protein mixture because of the huge amounts of peptides present in the sample. The same sample was treated with the lanthanum precipitation process and then treated with direct on-pellet digestion and measured by MALDI-TOF MS (bottom spectrum shown in FIG. 3). A Mascot search on the data by PMF analysis resulted in a sequence coverage of 87% for α-casein (15 peptides identified with a score of 128). No other protein was present or could be identified.
In order to test the developed strategy with a complex biological sample of relevance to clinical proteomics, cerebrospinal fluid (CSF) samples were analyzed using the lanthanum precipitation procedure followed by on-pellet trypsin digestion and micro liquid chromatography electrospray ionization mass spectrometry (μLC-ESI-MS). The peptide identification was done by MS/MS. CSF is in contact with the whole central nervous system, surrounds the entire brain, and acts as an immunological and mechanical protection for the brain inside the skull. Thus, CSF reflects the biochemistry of the brain and changes in its protein composition and concentration are thought to be relevant for understanding chronic degenerative neurological diseases like Alzheimer's disease (AD), Creutzfeldt-Jakob disease, and multiple sclerosis. The determination of the concentration of beta amyloid 1-42 and tau protein in CSF, for example, is an important tool in the diagnosis of AD.
The analysis resulted in the identification of 53 phosphorylated and/or glycosylated proteins (Table 3). Twenty out of these have been mentioned in the literature as potential biomarkers for AD (Table 3). Previous published methods for the biomarker analysis including enzyme-linked immunosorbent assay (ELISA) applications, which take at least two hours (Wuhrer, M. et al. (2005) J. Chromatogr. A. 825, 124-133; Moon, Y. U. et al. (2000) J. Solution Chem. 29, 8) to perform while having lower performance due to non-specific binding problems (Scherz, H. et al. (1998) Analytical Chemistry of Carbohydrates, 1st Ed., pp. 275-276, Georg Thieme Verlag, Stuttgart.) In addition only a defined number of proteins can be bound in one analytical run in previous methods, while the precipitation method of the invention enables the simultaneous binding of phosphorylated and glycosylated proteins. Proteins which were recently described as phosphorylated like serum albumin, Dickkopf-related protein 3, SPARC-like protein 1 and apolipoprotein E36 (ApoE) were also successfully detected by this method in one single run.







TABLE 3







List of the identified proteins in CSF by μ-LC ESI


MS/MS analysis after precipitation by Lanthanum (III).












Accession
Unique peptide
Sequence





number
detected
coverage %
Protein
Phosphorylation
Glycosylation















P02768
37
75.4
Serum albumin
+
−


P02787
28
46.7
Transferrin
+
+


P01024
20
19.9
Human complement
+
+





component C3


O14594
2
3.1
Neurocan
−
+





core protein


P01009
9
31.1
Alpha-1-antitrypsin
−
+


P06396
8
18.4
Gelsolin
+
−


P00751
4
7.9
Complement factor B
−
+


P02679
9
32.9
Fibrinogen gamma chain
−
+


P00738
3
12.1
Haptoglobin alpha chain
−
+


P0C0L4
11
13.0
Acidic complement C4
−
+


P05090
3
20.1
Apolipoprotein D
−
+


P02751
8
5.5
Fibronectin
+
+


P02675
3
7.5
Fibrinogen beta chain
−
+


P10909
8
23.6
Clusterin (ApoJ)
−
+


P00734
3
8.2
Prothrombin
−
+


P05155
8
17.2
Plasma protease C1
−
+





inhibitor


P01023
17
17.8
Alpha-2-macroglobulin
−
+


P02652
4
41.0
Apolipoprotein A - II
+
−


P05067
3
4.9
Amyloid beta A4 protein
+
+


P02649
9
41.3
Apolipoprotein E
+
+


P41222
4
36.3
Prostaglandin - H2 D-
−
+





isomerase


P01857
7
36.7
Ig gamma-1 chain C region
−
+


P02766
5
68.7
Transthyretin
−
+


P01042
4
8.5
Kininogen-1
+
+


Q14515
5
15.5
SPARC-like protein 1
+
+


P06727
2
5.5
Apolipoprotein A - IV
+
−


P04004
4
10.9
Vitronectin
+
+


P10451
5
27.7
Osteopontin
+
+


P02760
2
6.5
Alpha -1- microglobulin
−
+


Q8WXD2
4
12.2
Secretogranin-3
+
−


P01860
4
65.1
Ig gamma-3 chain C region
−
+


P00450
6
9.4
Ceruloplasmin
+
+



3
5.1
Neural cell adhesion
+
+





molecule 1


P13591
7
33.1
Dickkopf-related
+
+





protein 3


Q9UBP4


P02647
5
21.7
Apolipoprotein A-I
+
+


P01011
4
15.8
Alpha-1-antichymotrypsin
−
+


P01034
3
28.8
Cystatin-C
+
−


P10645
4
16.0
Chromogranin-A
+
+


P05060
8
17.6
Secretogranin-1
+
+


P01019
4
11.1
Angiotensinogen
−
+


P36955
5
13.6
Pigment epithelium-derived
+
+





factor


O94985
3
4.5
Calsyntenin-1
−
+


Q06481
5
10.9
Amyloid-like protein 1
+
−


P23142
2
5.7
Fibulin-1
+
+


P20774
2
3.8
Fibulin - 3
−
+


O00533
4
6.5
Neuronal cell adhesion
+
+





molecule L1 - like





protein


P02774
7
26.8
Vitamin D-binding protein
−
+


P61769
2
18.5
Beta -2 - microglobulin
+
+


Q9UHG2
3
21.9
Pro - SAAS
+
+


P02763
4
26.4
Alpha-1- acid
−
+





glycoprotein 1


Q99435
3
4.9
Protein kinase C-
−
+





binding protein





NELL 2


P02790
2
8.4
Hemopexin
−
+
















TABLE 4







List of identified proteins in CSF samples analyzed by La3+-precipitation


that have been found to be Alzheimer Disease (AD) related.











Accession






Number
Protein
Hit
Phosphorylation
Glycosylation





P05067
Amyloid beta A4
Ray, I. et al. (2000)
+
+



protein
Brain Research, 853,




344-351; Grimmer, T.




et al. (2009) Biol.




Psychiatry, 65, 927-




934


P06396
Gelsolin
Ray, I. et al. (2000)
+
−




Brain Research, 853,




344-351


P01011
Alpha-1-
Eriksson, S. et al.
−
+



Anti-chymotrypsin
(1995) Proc. Natl.




Acad. Sci. USA, 92,




2313-2317; Gollin, P.




et al. (1992) Neuro




Report, 3, 127-210


P02647
Apolipoprotein A-I
Yin, G. N. et al.
−
+




(2009) Brain




Research, 1265, 158-




170


P02679
Fibrinogen
Yin, G. N. et al.
−
+




(2009) Brain




Research, 1265, 158-




170; Ojien van, M. et




al. (2005) J. Am




Hearth Assoc. 36,




2637-2641


P00738
Haptoglobin
Yin, G. N. et al.
+
+




(2009) Brain




Research, 1265, 158-




170


P02766
Transthyretin
Biroccio, A. et al.
−
+




(2006) Proteomics,




6, 2305-2313


P10451
Osteopontin
Wung, J. K. et al.
−
+




(2007) Current




Alzheimer Res. 4,




67-72


Q14515
SPARC-like protein 1
Yin, G. N. et al.
+
+




(2009) Brain




Research, 1265, 158-




170


P02649
Apolipoprotein E
Corder, E. H. et al.
+
+




(1993) Science, 261,




921-923; Nguyen, T.




T. et al. (2000) J. Clin




Endocrinol. Metab.




85, 4354-4359


P10909
Clusterin
Yin, G. N. et al.
−
+




(2009) Brain




Research, 1265, 158-




170.


Q9UBP4
Dickkopf-related
Zenzmaier, C. et al.
+
+



protein 3
(2004) J. of




Neurochem. 110,




653-661


P36955
Pigment epithelium-
Yin, G. N. et al.
+
+



derived factor
(2009) Brain




Research, 1265, 158-




170


P02774
Vitamin D-binding
Yin, G. N. et al.
−
+



protein
(2009) Brain




Research, 1265, 158-




170


P01009
Alpha-1-antitrypsin
Gollin, P. et al.
−
+




(1992) Neuro




Report, 3, 127-210


P02763
Alpha-1-acid
Merritt, C. M. et al.
−
+



glycoprotein 1
(1988) Brain Res.




Reviews, 66, 97-106


P04004
Vitronectin
Rogers, J. et al.
+
+




(2000) Neurobiology




of Aging, 21, 383-421


P01042
Kininogen-1
Puchades, M. et al.
+
+




(2003) Molecular




Brain Res. 118, 140-




146


P01034
Cystatin-C
Mi, W. et al. (2009)
+
−




Nature Genetics, 39,




1440-1442


P10645
Chromogranin A
Lechner, T. et al.
+
+




(2004) Experimental




Gerontology, 39,




101-113









As discussed before, it is possible to precipitate both phosphoproteins and glycoproteins and recover them in two different fractions. It was discovered that lanthanum (III) will bind and precipitate glycoproteins with lower affinity than phosphoproteins and phosphate ions. This property was used to perform the separation. The phosphoprotein α-casein and the glycoprotein lectin from fava beans were dissolved in deionized water (0.1 μg/ml). To 200 μL of the sample, 2 μL of 1M lanthanum chloride solution and optionally 1 μL of 2 M potassium hydrogen phosphate solution were added. After precipitate formation and centrifugation, a pellet was obtained. The pellet was resuspended in 500 μl water to wash and remove non precipitated proteins. This washing step was repeated two more times. In a first experiment the proteins were recovered at the same time by dissolving the pellet in 42.5% phosphoric acid in order to verify if the glycoprotein was still bound after the washing steps. The supernatant was spotted on a MALDI target with sinapinic acid as matrix. It was confirmed by MALDI-TOF that the glycoprotein was bound to the pellet. The same experiment was repeated but after the washing steps the glycoprotein was eluted from the pellet by incubating the sample for 5 min at 800 rpm and 25° C. with 4 M potassium hydrogen phosphate solution. The supernatant with the eluted glycoprotein was collected and spotted on a MALDI target with sinapinic acid matrix confirming that the glycoprotein was released. It was confirmed that the precipitated phosphoprotein remained on the pellet. The pellet was dissolved in phosphoric acid, desalted and spotted on the MALDI target as described before. The phosphoprotein was detected but no glycoprotein was detected.
The MALDI-TOF spectra in FIG. 4 shows that phosphoproteins and glycoproteins can be recovered together with no loss of glycoprotein. Glycoproteins can be precipitated simultaneously by trivalent La cations and recovered. Spectrum (A) shows both a glycoprotein (peak 1, lectin from fava beans) and a phosphoprotein (peak 2 beta casein) before the precipitation process. Spectrum (B) is the measurement of the supernatant demonstrating the successful precipitation of both proteins with no protein in the supernatant. Spectrum (C) shows both types of proteins were recovered and measured after the dissolving the pellet.
Isolating phosphoproteins and/or glycoproteins in complex biological samples is challenging. The method depends on 1) discovering different selectivities of the two types proteins and 2) discovering some sort of separation procedure to take advantage of the selectivity differences. It was necessary to develop a targeted washing strategy for the selective isolation of phosphoproteins alone and glycoproteins alone. Among all the methods tried for removal of the glycosylated proteins from La3+ cations, a washing step of the precipitated pellet gave the best results. It was discovered that several types of wash solutions are effective. The wash solution could select for the glycoprotein. An example of this is boric acid or sodium borate solution. Alternatively, the wash solution could displace the glycoprotein. Examples of this are carbonate, oxalate, or hydroxide containing solutions. A solution of a K2HPO4 solution (e.g. 4 M) gave very good results and could be used to extract or elute the proteins from the metal precipitate pellet.
FIG. 5 shows the MALDI-TOF spectra of the experiment performed with the K2HPO4 solution as the washing buffer. It is important that no glycoproteins are lost during the first washing steps, and that they are recovered later. The spectra show fractionation is achieved by adding concentrated phosphate to the pellet (for example 4M KH2PO4 solution), which will displace the precipitated La-glycoprotein complexes. Spectrum (A) shows the two standard glycoprotein lectin and phosphoprotein casein before precipitation and spectrum (B) the supernatant after precipitation in which no protein could be detected. To check the stability of the La-glycoprotein coordination complexes during the standard pellet washing steps performed in the Table 4 and Table 5 outlined methods, the supernatants from the three washing steps were pooled, desalted and measured by MALDI-TOF. Spectrum (C) shows the glycoprotein (lectin) recovered after the addition of a 4 M KH2PO4 solution. Spectrum (D) is the MALDI-TOF spectrum of the recovered phosphoprotein α-casein after dissolving the pellet. No glycoprotein remained in the pellet.
The method fills an important need; the ability to isolate these proteins in a reproducible manner, especially when they are present at very low concentrations. Highly efficient, selective and reproducible capture is also necessary if the amount of protein present has to be quantified reliably. These results demonstrate that it is possible to simultaneously precipitate phosphoproteins and glycoproteins and then collect them in two different fractions for further analyses. It is also possible to precipitate phosphoproteins and glycoproteins at the same time and then separate the two fractions. Lanthanum will precipitate both types of proteins if the procedure is performed correctly. A metal such as terbium, europium, or erbium can be chosen to selectivity precipitate phosphoproteins.
Table 5 shows the conditions for the application of the method to urine samples.







TABLE 5







Method that was used for the isolation of phosphorylated and glycosylated proteins by Lanthanum


(III) precipitation from a urine sample. Urine samples usually require a larger volume to


recover suitable proteins. Other parameters of the method can be varied as well. Comments


and some of the possible variations on the method are included in the table.













Amount,




Step
Description
volume, etc.
Function
Comment














1
Urine sample was
1000 μL of

Centrifuging down



centrifuged first
sample

the cells contained in



for 20 min to


urine is the only pre-



pellet the cells.


treatment needed.


2
Protein
Add 20 μl 10
Ensured that all
DTT: Dithiotreitol



denaturation
mM DTT and
phospho-sites and
nOGP: 1-O-n-octyl-β-




20 μl 45 mM
glyco-sites are
D-glucopyranoside




nOGP to the
accessible to bind to




sample
the metal


3
Further
Incubated
Broke up the



denaturation
the sample
disulfide bonds by




for 5 min at
DTT




95° C.


4
Protein alkylation
Added 20 μl
Alkylated the thiol-
IAA: Iodoacetamide




of 50 mM IAA
groups
Ensured that the




and

disulphide bonds




incubated 30

were not reformed.




min in the




dark


5
Added Lanthanum
5 μL of 1M
Formed insoluble
Cloudy solution was



(III), mixed sample
LaCl3 solution
salt complex with
formed with some



by vortex

phosphorylated
particulate falling to





proteins
the bottom of vial.





Formed insoluble





chelate complex





with glycosylated





proteins.


6
Added K2HPO4,
4 μL of 2M
Formed additional
The pellet was more



mixed sample by
K2HPO4
ppt with unbound
stable and easier to



vortex
solution
La cation. La(PO4)
form.





ppt increased the





size of the pellet.


7
Centrifuged


8
Re-suspended and
500 μL of 8M
Denatured
Also, removed any



washed the pellet
urea/1%
precipitated
non-specific



once with urea
CHAPS in PBS
proteins in pellet
adsorbed protein.



and CHAPS in PBS
pH 7.4

Surfactant may also



buffer and then


be added earlier with



centrifuged again


the addition of






Lanthanum.


9
Re-suspended and
500 μl de-
Removed excess



washed the pellet
ionized water
urea and surfactant



3 times with DI

and non specific



water, centrifuging

adsorbed proteins.



after each wash

The excess





denaturing agents





would otherwise





affect the on-pellet





tryptic digest.


10
Treated pellet with
50 μL of 4M
Broke up any La-
The pellet was



phosphate ion and
K2HPO4 and
glycoprotein
treated with a



optionally keep
de-ionized
precipitate that was
centrifugation



supernatant
water
formed in Step 2.
method (but





No extra co-ppt
filtration could also





with
be used).





phosphorylated





proteins is formed





because excess La





(III) has been





removed.





Phosphoproteins





remain in pellet.





Glycoproteins are in





supernatant, can be





further analyzed by





top down or bottom





up analysis.


11
On pellet
Several steps
Released mostly
Bottom up sample



microwave-

non-
preparation for



assisted trypsin

phosphorylated
protein identification



digestion

peptides through
by MS/MS analysis.





digestion from the





pellet


12
μLC-ESI-MS


Bottom up analysis



analysis or other



method on



supernatant


Optional
Re-dissolve pellet
20 μL of 10%
Releases the
Top down sample


13

TFA
phosphoproteins
preparation


Optional
SDS-PAGE, MS or


Top down analysis


14 (follows
other instrumental


optional
analysis method


13)









Having now generally described the invention, the same will be more readily understood through reference to the following examples, which are provided by way of illustration, and are not intended to be limiting of the present invention, unless so specified.
EXAMPLES
The following preparations and examples are given to enable those skilled in the art to more clearly understand and practice the present invention. They should not be construed as limiting the scope of the invention, but merely as being illustrative and representative thereof.
Example 1
Precipitation of Phospho-Proteins Using Lanthanum Chloride
A protein mixture consisting of α-casein (0.1 μg/μL), lysozyme (1 μg/μL), myoglobin (1 μg/μL), and BSA (1 μg/μL) (all purchased from Sigma-Aldrich, St. Louis, Mo., USA) was used as the standard sample. 1.5 μL of a lanthanum chloride solution (1M) (puriss. p.a., Sigma-Aldrich, St. Louis, Mo., USA) was added to 50 μL of the sample followed by vortex-mixing. In a further step, 1.5 μL of a 2 M K2HPO4 (99.9%, Sigma-Aldrich, St. Louis, Mo., USA) solution were added. The resultant precipitate was centrifuged and washed by resuspending the pellet once in 500 μL of a mixture of 8 M urea solution and 1% CHAPS in PBS buffer (pH 7.4) (>98% Sigma-Aldrich, St. Louis, Mo., USA) and then washed three more times in 500 μL deionized water. In order to elute the bound proteins, the pellet was dissolved in 10 μL of 2.5% H3PO4 and analyzed by MALDI-TOF (FIG. 1).
Example 2
On-Pellet Microwave-Assisted Tryptic Digest of Lanthanum Pellets
The pellet from Example 1 was used to perform an on-pellet microwave-assisted tryptic digest. The procedure provided a direct screening of the bound proteins on the pellet. After the washing steps and centrifugation, 16 μL of NH4HCO3 solution (0.5 M) (99.5%, Sigma-Aldrich, St. Louis, Mo., USA), 6 μL nOGP (45 mM) (98% Sigma-Aldrich St. Louis, Mo., USA), and 5 μL DTT (10 mM) (>99%, Sigma-Aldrich, St. Louis, Mo., USA) were added to the lanthanum phosphate pellet which was then incubated at 99° C. and 800 rpm for 5 minutes. After cooling to room temperature, 5 μL of IAA (50 mM) (crystalline, Sigma-Aldrich, St. Louis, Mo., USA) were added, and the sample was put in the dark for 30 minutes. In the next step, 70 μL of deionized water and 8 μL of trypsin (0.1 μg/μL) (sequencing grade modified, Promega, Mannheim, Germany) were added. The sample was then transferred to the microwave, and the digestion process was performed at 70 W for 2 minutes. The tryptic activity was stopped by the addition of 30 μL TFA (2%) (purum≧99.5%, Fluka (Buchs, Switzerland). Finally, 1 μL of the sample was spotted on a MALDI stainless steel target (Bruker Daltonics GmbH, Bremen, Germany), followed by the addition of 1 μL of 2,5-dihydroxybenzoic acid.
Example 3
Analysis of CSF by Lanthanum (III) Precipitation
Twenty CSF samples were analyzed by lanthanum (III) precipitation. Excess CSF samples from the clinical routine at the Clinical Neurochemistry Laboratory at the Sahlgrenska University Hospital (Sweden) were included in the study. The CSF was obtained by lumbar puncture that was performed on clinical indication to exclude infections or inflammatory diseases of the central nervous system. The CSF was immediately transported to the laboratory, centrifuged at 1600×g for 10 min, aliquoted and stored at −80° C. pending analysis. Samples with greater than 500 erythrocytes/μL were excluded from the study. To 100 μL of each sample, 8 μL of a lanthanum chloride solution (1M) were added. After a short vortex mixing, 8 μL of a potassium hydrogen phosphate solution (2 M) were added to increase the stability of the resulting pellet as well as to bind excess lanthanum (III). At this time, a smaller amount of the phosphate solution was added in order not to inhibit the lanthanum (III) glycoprotein interactions.
In another set of experiments 1 mL CSF was used and 6 μl of 1M lanthanum chloride and 5 μL 2M potassium hydrogen phosphate solution was used. The precipitate was then centrifuged and washed four times First with 500 μL of a mixture of 8 M urea solution and 1% CHAPS in PBS buffer (pH 7.4) and then with 500 μL deionized water. In order to digest the bound proteins, the same protocol as described before was used. The resulting peptides were then analyzed by μLC-ESI-MS and identified by Sequest against the SwissProt Homo sapiens database.
MALDI-TOF MS, μLC-ESI-MS and MS/MS analysis including database searching analysis was used for the sample analysis. For the sample preparation, 1 μL of the digest was spotted on a stainless steel target (Bruker Daltonics GmbH, Bremen, Germany) followed by adding 1 μL of a saturated dihydroxybenzoic acid matrix (50% ACN solution containing 1% H3PO4 and 0.1% TFA). For the measurement of the intact proteins, sinapinic acid (50% ACN solution containing 0.1% TFA) was applied as matrix. All the measurements were recorded by an Ultraflex I (Bruker Daltonics, Germany) MALDI-TOF/TOF MS in reflector and lift mode for MS/MS analysis. All mass spectra were recorded by summing 400 laser shots. The laser power was adjusted to between 30 and 50% of its maximal intensity, using a 337-nm nitrogen laser with a frequency of 50 Hz. The Flex Analysis version 2.4 and BioTools 3.0 software packages provided by the manufacturer were used for data processing. Peptide database searching analysis was performed with Mascot software (http://matrixscience.com) and Swiss Prot as database. For MS/MS search, the parameters were the following: C-carbamidomethyl (fixed modification), M-oxidation, ST-phosphorylation, Y— phosphorylation (variable modification), mass value (monoisotopic), peptide mass tolerance (120 ppm), mass tolerance (0.6 Da), missed cleavage (1), taxonomy (“mammalian” and for lysozyme “all entries”) and a S/N threshold of 4.
For the μLC-ESI-MS analysis, tryptic digests were separated using micro high performance liquid chromatography and analyzed by electrospray lontrap MS and MS/MS. The Agilent 1100 series system included a Nanoflow Pump with micro vacuum degasser, a Capillary Pump with micro vacuum degasser, a thermostated micro well-plate autosampler, a thermostated column compartment, and an external 2 position/10-port valve (Agilent Technologies, Santa Clara, Calif., USA). A 300 μm ID×5 mm 5 μm C18 100 Å PepMap trapping column and an Acclaim PepMap C18, 3 μm, 100 Å, 75 μm ID, and 50 cm separation column (Dionex, Amsterdam, The Netherlands) were used. After the loading of 7 μL onto the trapping column with a flow rate of 20 μL/min for 3 min, the separation was performed under reversed phase conditions with solvent A 2% acetonitrile (ACN) and 0.1% (v/v) formic acid (FA) in water and solvent B 0.1% (v/v) FA in 80% ACN, at a flow rate of 150 nL/min and 40° C. A linear gradient (50 min from 0% B to 60% B) was used. Hyphenation to the mass spectrometer was carried out by a nanoflow ESI source from Proxeon (Odense, Denmark) with Pico Tips from New Objective (FS360-20-10, MA, USA). Mass spectrometric data were obtained on the linear ion trap LTQ from Thermo Fisher (Thermo Fisher Scientific Inc., Waltham, Mass.). Measurements were performed as follows: source voltage −1.7 kV, capillary temperature 220° C., capillary voltage 22 V, and tube lens 129 V.
Data acquisition and interpretation was done with Xcalibur from Thermo Fisher (Thermo Fisher Scientific Inc., Waltham, Mass.). Database search was carried out with Bioworks Browser 3.3.1 SP1 (Thermo Fisher Scientific Inc., Waltham, Mass.) and Sequest against the SwissProt Homo sapiens database updated Apr. 22, 2010, 20166 sequences, carbamidomethylation on C as fixed modification, as well as oxidation of M and phosphorylation of S, T and Y as variable modifications and 3 possible missed cleavage sites. The following parameters were used to filter the identified proteins: for peptides a Sf—final score of 0.65, Xcorr vs. charge state for single, double and triple charged 1.50, 1.60, 2.00, 3.00 and a peptide probability of 1×10−4; for proteins a Sf—final score of 2.00 and a minimum number of 2 distinct peptides per protein. To obtain a high confidence for the identified proteins the peptides were checked manually and a reverse data base search was performed. The reverse data base search resulted in 0% false positive confirming the filter as used was reliable.

## Claims

1. A method for isolating phosphoproteins from a biological sample, comprising:
a. providing a biological sample comprised of denatured phosphoproteins, wherein the denatured phosphoproteins are not digested;
b. providing a lanthanum metal cation;
c. mixing the biological sample with the lanthanum metal cation, wherein at least a portion of lanthanum metal cation forms a first precipitate with the phosphoproteins in the biological sample;
d. adding a phosphate anion to the first precipitate obtained from step (c) to form a second precipitate; and
e. recovering the first precipitate from step (c) and the second precipitate from step (d).

2. The method of claim 1, wherein the first precipitate and the second precipitate are recovered by centrifugation.

3. The method of claim 2, wherein following step (e), the first precipitate and the second precipitate are washed.

4. The method of claim 3, wherein the wash is comprised of water, PBS, CHAPS or urea.

5. The method of claim 4, wherein the first and second precipitates are solubilized.

6. A method of isolating phosphoproteins from a biological sample, comprising:
a. providing a biological sample, wherein the biological sample is comprised of denatured phosphoproteins, wherein the denatured phosphoproteins are not digested;
b. providing a lanthanum metal cation;
c. providing a phosphate anion;
d. mixing the biological sample with the lanthanum metal cation and the phosphate anion, wherein at least a portion of lanthanum metal cation forms a precipitate with the phosphate anion and the denatured phosphoproteins in the biological sample; and
e. recovering the precipitate.

7. The method of claim 6, wherein the precipitate is recovered by centrifugation.

8. The method of claim 7, wherein following step (d), the precipitate is washed.

9. The method of claim 8, wherein the wash is comprised of water, PBS, CHAPS or urea.

10. The method of claim 9, wherein the precipitate is solubilized.