# Patent

## Title
Salute lettuce variety

## Abstract

A new lettuce variety designated ‘Salute’ is described. ‘Salute’ is an iceberg lettuce variety exhibiting stability and uniformity.

## Background
CROSS REFERENCE TO RELATED APPLICATION
This application claims the benefit under 35 § USC 119(e) of prior U.S. Provisional Patent Application No. 61/480,989, filed Apr. 29, 2011, the disclosure of which is hereby incorporated by reference in its entirety.


FIELD OF THE INVENTION
This invention relates to the field of plant breeding. In particular, this invention relates to a new lettuce, Lactuca sativa, variety, ‘Salute’.
BACKGROUND OF THE INVENTION
Lettuce is an increasingly popular crop. Worldwide lettuce consumption continues to increase. As a result of this demand, there is a continued need for new lettuce varieties. In particular, there is a need for improved iceberg lettuce varieties that exhibit vigorous growth, increased weight, and increased yield.
SUMMARY OF THE INVENTION
In order to meet these needs, the present invention is directed to an improved iceberg lettuce variety that exhibits vigorous growth, increased weight, and increased yield. In particular, the present invention is directed to Lactuca sativa seed designated as ‘Salute’ having ATCC Accession Number PTA-13110. The present invention is further directed to a Lactuca sativa plant produced by growing ‘Salute’ lettuce seed having ATCC Accession Number PTA-13110. The present invention is further directed to a lettuce head isolated from a Lactuca sativa plant produced by growing ‘Salute’ lettuce seed having ATCC Accession Number PTA-13110. The present invention is further directed to a Lactuca sativa plant having all the physiological and morphological characteristics of a Lactuca sativa plant produced by growing ‘Salute’ lettuce seed having ATCC Accession Number PTA-13110. The present invention is further directed to an F1 hybrid Lactuca sativa plant having ‘Salute’ as a parent, where ‘Salute’ lettuce seed is grown from ‘Salute’ seed having ATCC Accession Number PTA-13110.
The present invention is further directed to pollen isolated from ‘Salute’ lettuce plants. The present invention is further directed to ovules isolated from ‘Salute’ lettuce plants. The present invention is further directed to tissue culture of ‘Salute’ lettuce plants.
The present invention is further directed to a method of selecting lettuce by: a) growing more than one ‘Salute’ lettuce plant, where the plants are grown from lettuce seed having ATCC Accession Number PTA-13110; and b) selecting a plant from step a). The present invention is further directed to lettuce plants and seeds produced there from wherein the lettuce plant is isolated by the selection method of the invention.
The present invention is further directed to a method of breeding lettuce by crossing a lettuce plant with a plant grown from ‘Salute’ lettuce seed having ATCC Accession Number PTA-13110. The present invention is further directed to lettuce plants and seeds produced therefrom, where the lettuce plant is isolated by the breeding method of the invention.


DETAILED DESCRIPTION OF THE INVENTION
In order to more clearly understand the invention, the following definitions are provided:
Iceberg Lettuce: Iceberg lettuce, Lactuca sativa L. var. capitala L. is also known as ‘crisp head’ lettuce. Iceberg lettuce is a lettuce plant type that forms a firm, spherical head formed with tightly folded brittle textured foliage. Internal color ranges from white to yellow to light green. The wrapper leaves surrounding the head are wider than they are long. Leaf margins can vary by type, being entire, undulating, or frilled. Wrapper leaf color ranges from yellow green to dark green.
Core Length Core length is the length of the internal lettuce stem. Core length is measured from the base of the cut head to the tip of the core.
Core Diameter Core diameter is the diameter of the lettuce stem at the base of the cut head.
Head Diameter Head diameter is the diameter of the vertically sliced lettuce plant head at its widest horizontal point, perpendicular to the stem.
Head Length Head length is the diameter of the vertically sliced lettuce plant head as measured from the base of the cut stem to the cap leaf.
Average Head Diameter Average head diameter is an average of the measured head diameter and head length of the lettuce head.
Average Head Diameter:Core Length The ratio of the average head diameter to core length is indicative of the percentage of useable product produced by the lettuce plant.
Frame Diameter The frame diameter is a measurement of the lettuce plant diameter at its widest point. The measurement of frame diameter is from the outer most wrapper leaf tip to outer most wrapper leaf tip.
Head Weight Weight of the marketable lettuce plant, cut and trimmed to market specifications.
Rogueing: Rogueing is the process in seed production where undesired plants are removed from a variety. The plants are removed since they differ physically from the general desired expressed characteristics of the variety. The differences can be related to size, color, maturity, leaf texture, leaf margins, growth habit, or any other characteristic that distinguishes the plant.
Market Stage: Market stage is the stage when a lettuce plant is ready for commercial lettuce harvest. In the case of an iceberg variety, the head is solid, and has reached an adequate size and weight.
Taking into account these definitions, the present invention is directed to seed of the lettuce variety ‘Salute’, plants produced by growing ‘Salute’ seed, plants selected from a collection of ‘Salute’ plants and seed derived or produced therefrom; and plants produced by crossing a ‘Salute’ lettuce plant with second lettuce plant, and seed derived or produced therefrom.
Origin and Breeding History of the Variety ‘Salute’
‘Salute’ is an iceberg lettuce variety developed from a hand pollinated cross of the Progeny Advanced Genetics breeding lines PSJV032083 and PSSJV032084. The female parent PSJV032083, was selected for its excellent resistance to tip burn and bolting, while demonstrating a thick leaf texture and excellent heading characteristics. The male parent PSJV032084, a Salinas type iceberg was also selected for its improved resistance to tip burn and bolting, as well as its adaptability to the summer growing conditions of the Salinas valley, California. The two parental varieties were selected for their specific adaptability and physiological resistances, and their respective yield potential. Through the single seed descent breeding method, we have developed a medium heading, tip burn and bolting resistant lettuce variety for the summer production regions of the southern Salinas valley in California, and the spring harvest period of the production region of Yuma Ariz.
In Year one, in a summer research seed production field in the San Joaquin valley in California, a cross was made of the breeding lines PSJV032083 and PSJV032084. The F1 seed was harvested in the fall of Year one and designated as PSJV032083XPSJV032084.
In October of Year two forty F1 seeds of PSJV032083XPSJV032084 were planted in a green house facility in San Juan Bautista, Calif., identified as research line number PSJB032319. At market maturity all apparent self pollinated plants were removed and the remaining F1 plants were allowed to self pollinate, the F2 seed was harvested in bulk in late April of Year two. The seed was immediately cleaned, processed, blended, and prepared for planting.
Two hundred random F2 seeds of line number PSJB032319 were planted in a summer research seed production field, and redesignated as PSJV042985. Segregation amongst the F2 population was noted and all plants were allowed to self pollinate and produce seed. The F3 seed from each plant was harvested and packaged individually in the fall of Year two. One seed from each package corresponding to an individual plant was removed and placed in one envelope and designated as PAUS043608, and planted again in a research production field in Australia in October of Year two. The planted research production field in Australia was observed, where segregation for phenotype and maturity was again evident and noted, and all plants were allowed to self pollinate and produce seed. F4 seed from 100 individual plants was harvested, cleaned, and packaged individually. The packets were then shipped from Australia to Salinas, Calif. for processing.
The 100 F4 lines, all from non-selected single F3 plants of the pedigree PSJV032083XPSJV032084 was processed in Salinas, Calif. in the summer of Year three. A trial was prepared containing each of the 100 F4 individual lines of the designated pedigree, using the parent varieties and industry standard varieties as checks for the desired attributes. A research trial was planted in King City, Calif. in July of Year three where the lines could be evaluated for the desired heat resistant traits. The trial was evaluated in September of Year three. All F4 lines were evaluated based on phenotypic uniformity, improved size, improved weight, improved frame size, resistance to tip burn, and core length when compared to the parent and check varieties. Twelve F4 lines of this pedigree were selected as they out performed the parent varieties, their sibs, and the majority of other lines in the trial for the designated traits. The PAUS 043608-57 line was among the 12 F4 lines advanced.
Seed from these 12 F4 lines were then increased in a Year three research and development seed production field in Australia. A sample of the remnant F4 seed from PAUS 043608-57, designated as PAUS054577, was planted in a research seed production field in October of Year three. The block contained roughly 200 plants, which were rogued for phenotype and maturity at various stages of growth, all offtypes and variants were removed. The remaining plants in the block were allowed to self pollinate, and the F5 seed from the block was harvested in mass in the spring of Year four.
Three trials of the 12 advanced breeding lines, parent lines, and commercial standard varieties, containing roughly 300 plants each were planted in the summer of Year four in the southern region of the Salinas valley, California for further evaluation for the desired traits. Evaluations of the trials were conducted in September and October of Year four. All 24 F5 advanced breeding lines were evaluated based on phenotypic uniformity, improved weight, Improved frame size, improved leaf texture, and resistance to tip burn and bolting when compared to the parent and check varieties. Four of the twelve F5 advanced breeding lines continually out performed the parent lines and the check varieties for the desired traits and were advanced. Among the four lines advanced was line number PAUS054577.
A portion of the remnant F5 seed from the five advanced lines was again increased in a Year five San Joaquin valley, California research and development seed production field. Roughly 200 plants of each line were grown. Line PAUS054577, was redesignated as PSJV075480, and all lines were rogued at multiple stages of development for phenotypic uniformity, head size, frame size and timing of bolting and flowering. The F5 line redesignated as PSJV075480 was noted to be uniform, stable, and free of variants during the production cycle. The F6 seed of the five lines was harvested as a priority in August of Year five, and was cleaned and processed. Multiple trials of the five F6 lines, including PSJV075480, were conducted in the warmer regions of the Salinas valley in California. Again, side-by-side comparisons with the parent varieties and commercial standard varieties were made. Line PSJV075480 continually out performed its siblings, its parents, and the standard check varieties for overall appearance, leaf texture, frame size, and resistance to tip burn and bolting. Based on the trial results, line PSJV075480 was advanced through the research process and designated as PX 1565.
Large trials of PX 1565 were then conducted in multiple lettuce production regions, including the southern region or the Salinas valley, California, and for the spring harvest slot in the Yuma production regions of Arizona. The variety continued to perform and the F6 generation of the variety was evaluated to be uniform, stable, and free of variants. An additional small increase of seed was made in a summer research and development seed production field in the San Joaquin valley, California. F7 seed was harvested in the fall of Year seven. Based on the continued performance of the variety PX 1565 was designated ‘Salute’ in February of Year eight.
‘Salute’ has been observed in large commercial plantings and in seed production fields to be uniform, stable, and free of variants for 2 generations.
A. Variety Description Information














Plant Type:
Iceberg









Seed




Seed Color:
White



Light Dormancy:
No



Heat Dormancy:
Yes



Cotyledons



Shape of Cotyledons:
Intermediate



Shape of Fourth Leaf:
Spatulate



Length/Width Index of Fourth Leaf:
22



Apical Margin:
Moderately Dentate



Basal Margin:
Finley Dentate



Undulation:
Medium



Green Color:
Dark



Anthocyanin:
Absent



Distribution:
None



Rolling:
Absent



Cupping:
uncupped



Reflexing:
Slight



Mature Leaves



Margin



Incision Depth (Deepest penetration of
Moderate



the margin):



Indentation (Finest Division of the
Crenate



Margin):



Undulation of the Apical Margin:
Moderate



Green Color:
Dark



Anthocyanin



Distribution:
None



Size:
Medium



Glossiness:
Moderate



Blistering:
Moderate



Leaf Thickness:
Thick



Trichomes:
Absent










B. Comparison to Most Similar Variety













Characteristic
‘Salute’
‘Laguna Fresca’







Spread of Frame Leaves
 34 cm
 39 cm


Head Diameter (market trimmed with
 13 cm
 15 cm


single cup leaf)


Head Shape
Spherical
Spherical


Head Size Class
Medium
Large


Head Count per Carton
 24
 24


Head Weight
896
952


Head Firmness
Firm
Firm


Butt


Shape
Rounded
Flat


Midrib
Flat
Moderately Raised


Core (Stem of Market-trimmed Head)


Diameter at the base of the Head
 4.0 cm
 4.0 cm


Ratio of Head Diameter/Core
 3.46
 3.75


Diameter


Core Height from base of Head to
 3.3 cm
 4.3 cm


Apex


Number of Days from First Water
 72
 66


Date to Seed Stalk Emergence


(Summer condition)


Bolting Class
Very Slow
Slow


Height of Mature Seed Stalk
100 cm
120 cm


Spread of Bolter Plant
 33 cm
 48 cm


Bolter Leaves
Curved
Straight


Margin
Dentate
Dentate


Color
Dark Green
Medium Green


Bolter Habit


Terminal Inflorescence
Present
Present


Lateral Shoots (above head)
Absent
Present


Basal Side Shoots
Absent
Absent


Adaptation Regions
Huron, CA;
Huron, CA;



Yuma, AZ
Yuma, AZ









C. Growing Season
















Season
‘Salute’
‘Laguna Fresca’









Spring area
Desert South West
Not Adapted



Summer area
Salinas Valley
Salinas Valley



Fall area
Not Adapted
Not Adapted



Winter area:
Not Adapted
Not Adapted










D. Diseases and Stress Reactions
















Disease or Stress





Virus
‘Salute’
‘Laguna Fresca’









Big Vein:
Susceptible
Susceptible



Lettuce Mosaic:
Susceptible
Susceptible










E. Fungi/Bacteria













Fungal/Bacterial
‘Salute’
‘Laguna Fresca’







Corky Root Rot (Pythium
Susceptible
Tolerant


Root Rot):


Downy Mildew (Races I,
Susceptible
Susceptible


IIA, III):


Powdery Mildew:
Susceptible
Susceptible


Sclerotinia Rot:
Intermediate
Intermediate


Bacterial Soft Rot
Susceptible
Susceptible


(Pseudomonas spp. &


others): Not tested


Botrytis (Gray Mold):
Susceptible
Susceptible


Other: Corky Root Rot
Susceptible
Susceptible


(Rhizomonas suberifaciens):









F. Insects
















Insects
‘Salute’
‘Laguna Fresca’









Cabbage Loopers:
Susceptible
Susceptible



Root Aphids:
Susceptible
Susceptible



Green Peach Aphid:
Susceptible
Susceptible










G. Physiological/Stress
















Stress
‘Salute’
‘Laguna Fresca’









Tipburn
Resistant
Resistant



Heat
Resistant
Intermediate



Drought
Susceptible
Susceptible



Cold
Susceptible
Susceptible



Salt
Susceptible
Susceptible










H. Post Harvest













Characteristic
‘Salute’
‘Laguna Fresca’







Pink Rib
Moderately Susceptible
Moderately Susceptible


Russet Spotting
Moderately Susceptible
Moderately Susceptible


Rusty Brown
Moderately Susceptible
Moderately Susceptible


Discoloration


Internal Rib
Moderately Susceptible
Moderately Susceptible


Necrosis (Black-


heart, Gray Rib,


Gray Streak)









Breeding and Selection

The present invention is further directed to the use of ‘Salute’ lettuce in breeding and selection of new varieties.
A. Breeding
In lettuce breeding, lines are selected for their appropriate characteristics. For example, one line may be selected for bolt tolerance in the fall growing conditions of the desert production locations of California and Arizona. Another line may be selected for the size, color, and texture of the lettuce head. Crosses are made, for example, to produce a dark green, sure heading iceberg lettuce with improved texture, and size for fall plantings in Yuma Ariz., and Huron Calif.
To optimize crossing, it is important to note that lettuce is an obligate self-pollinating species. This means that the pollen is shed before stigma emergence, assuring 100% self-fertilization. Since each lettuce flower is an aggregate of about 10-20 individual florets (typical of the Compositae family), manual removal of the anther tubes containing the pollen may be performed by procedures well known in the art of lettuce breeding.
In addition to manual removal of anther tubes, A modified method of misting to wash the pollen off prior to fertilization may be used to assure crossing or hybridization. About 60-90 min past sunrise, flowers to be used for crossings are selected. The basis for selection are open flowers, with the stigma emerged and the pollen visibly attached to the single stigma (about 10-20 stigma). Using 3-4 pumps of water from a regular spray bottle, the pollen are washed off with enough pressure to dislodge the pollen grains, but not enough to damage the style. Excess water is dried off with clean paper towels. About 30 min later the styles should spring back up and the two lobes of the stigma are visibly open in a “V” shape. Pollen from another variety or donor parent are then introduced by gently rubbing the stigma and style of the donor parent to the maternal parent. Tags with the pertinent information on date and pedigree are then secured to the flowers.
About 2-3 weeks after pollination, seeds are harvested when the involucre have matured. The seeds are eventually sown and in the presence of markers such as leaf color or leaf margins, the selfed or maternal seedlings or plants are identified. Generally, there are no visible markers and breeders must wait until the F2 generation when expected segregation patterns for the genetic character of interest can be followed. This latter situation mandates a lengthy wait to determine if hybrids are produced. Two useful references teaching the methods for out crossing lettuce are: (1) Ryder, E. J. and A. S. Johnson. 1974. Mist depollination of lettuce flowers. Hortscience 9:584; and (2) Nagata, R. T. 1992. Clip and Wash Method of Emasculation for Lettuce. Hortscience 27(8):907-908.
B. Selection
In addition to crossing, selection may be used to isolate lettuce new lettuce lines. One or more lettuce seeds are planted, the plants are grown and single plant selections are made of plants with desired characteristics. Such characteristics may include improved head and frame size, deeper or darker green leaf color, etc. Seed from the single plant selections are harvested; separated from seeds of the other plants in the field and re-planted. The plants from the selected seed are monitored to determine if they exhibit the desired characteristics from the originally selected line. Selection work is continued over multiple generations to increase the uniformity and size of new line.
DEPOSIT INFORMATION
A deposit of the lettuce variety ‘Salute’ is maintained by Progeny Advanced Genetics, having an address at 590A Works Street, Salinas, Calif. 93901, United States of America. Access to this deposit will be available during the pendency of this application to persons determined by the Commissioner of Patents and Trademarks to be entitled thereto under 37 C.F.R. §1.14 and 35 U.S.C. §122. Upon allowance of any claims in this application, all restrictions on the availability to the public of the variety will be irrevocably removed by affording access to a deposit of at least 2,500 seeds of the same variety with the American Type Culture Collection, (ATCC), ATCC Patent Depository, 10801 University Boulevard, Manassas, Va., 20110, USA.
At least 2500 seeds of lettuce variety ‘Salute’ were deposited on Aug. 1, 2012 according to the Budapest Treaty in the American Type Culture Collection (ATCC), ATCC Patent Depository, 10801 University Boulevard, Manassas, Va., 20110, USA. The deposit has been assigned ATCC number PTA-13110. Access to this deposit will be available during the pendency of this application to persons determined by the Commissioner of Patents and Trademarks to be entitled thereto under 37 C.F.R. §1.14 and 35 U.S.C. §122. Upon allowance of any claims in this application, all restrictions on the availability to the public of the variety will be irrevocably removed.
The deposit will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if a deposit becomes nonviable during that period.
This invention will be better understood by reference to the following non-limiting Examples.
EXAMPLES
Example 1
General Trialing Method
I. Set Up
1. Parental lines and competing varieties are identified.
2. Primary slots are identified.
3. Necessary accession lines are located and purchased/received from seed dealers or growers.
4. All varieties are assigned a number to maintain integrity and anonymity.
5. Trials are set up in with all necessary varieties. Variety arrangement for trial is diagramed.
II. Planting
1. Commercial plantings are located by contacting commercial growers during the planting slot recommended for the variety.
2. A field is located during commercial planting, and the necessary rows and area is marked off.
3. Varieties are planted according to a diagram in 100 ft. ranges.
4. All varieties are planted in same manner to mimic the planting of the commercial variety as closely as possible.
5. A trial map is drawn diagramming the trial, the trial location in the field and directions to the field.
III. Maintenance
1. All varieties are treated identically. Plants are watered, fertilized, and treated to control pests in the same manner as the commercial field.
2. The trial is thinned to separate the plants for optimum growth.
Example 2
Comparative Analysis
Following the procedures of Example 1, ‘Salute’ iceberg lettuce was compared to its most similar variety. The data are presented in Tables 1 and 2. Table 1 shows trial data comparing core lengths of ‘Salute’ iceberg lettuce variety and its most similar variety ‘Laguna Fresca’. Table 2 shows trial data comparing head diameters of ‘Salute’ iceberg lettuce variety and its most similar variety ‘Laguna Fresca’.







TABLE 1





Evaluation of core length of ‘Salute’ and most similar variety ‘Laguna Fresca’

















Trial No.











1
2
3









Location











Summer Year 8
Summer Year 8
Summer Year 8



King City, CA
King City, CA
King City, CA



Core Length (mm)
Core Length (mm)
Core Length (mm)













Plant
Salute
Laguna Fresca
Salute
Laguna Fresca
Salute
Laguna Fresca





 1
35.0
44.0
35.0
45.0
25.0
45.0


 2
35.0
50.0
35.0
45.0
25.0
45.0


 3
35.0
40.0
35.0
45.0
25.0
45.0


 4
40.0
45.0
30.0
40.0
20.0
35.0


 5
35.0
45.0
35.0
50.0
25.0
35.0


 6
35.0
40.0
30.0
40.0
20.0
35.0


 7
40.0
40.0
30.0
35.0
25.0
35.0


 8
35.0
40.0
30.0
45.0
25.0
40.0


 9
35.0
40.0
30.0
40.0
30.0
40.0


10
35.0
40.0
35.0
40.0
20.0
40.0


11
40.0
45.0
30.0
40.0
20.0
40.0


12
35.0
45.0
30.0
45.0
25.0
35.0


13
30.0
40.0
35.0
40.0
20.0
35.0


14
30.0
40.0
35.0
45.0
20.0
30.0


15
30.0
40.0
30.0
45.0
20.0
35.0


16
35.0
45.0
30.0
35.0
15.0
45.0


17
35.0
40.0
30.0
40.0
25.0
35.0


18
35.0
40.0
35.0
40.0
25.0
40.0


19
30.0
35.0
35.0
40.0
25.0
35.0


20
30.0
35.0
35.0
35.0
20.0
35.0


21
30.0
40.0
30.0
50.0
20.0
35.0


22
30.0
45.0
35.0
40.0
25.0
30.0


23
35.0
45.0
30.0
40.0
25.0
30.0


24
30.0
40.0
25.0
35.0
20.0
45.0


25
35.0
40.0
30.0
30.0
20.0
45.0


26
30.0
45.0
35.0
50.0
20.0
30.0


27
35.0
40.0
26.0
50.0
25.0
30.0


28
35.0
45.0
30.0
35.0
25.0
30.0


29
35.0
45.0
30.0
30.0
30.0
35.0


30
30.0
40.0
30.0
50.0
25.0
35.0


Average
33.8
41.8
31.7
41.3
23.0
36.8


stan dev
3.13E+00
3.32E+00
2.96E+00
5.71E+00
3.37E+00
5.17E+00


T test
9.61E−05

2.84E−11

9.01E−18



Probability %
100.0

100.0000

100.0000



% Difference
−19.1

−23.3

−37.6



Confidence Int
0.0358
0.0380
0.0339
0.0654
0.0386
0.0592


Range of Var min*
33.80
41.76
31.67
41.27
22.96
36.77


Range of Var max*
33.87
41.84
31.73
41.40
23.04
36.89












Trial No.











4
5
6









Location











Summer Year 9
Summer Year 9
Summer Year 9



King City, CA
King City, CA
King City, CA



Core Length (mm)
Core Length (mm)
Core Length (mm)













Plant
Salute
Laguna Fresca
Salute
Laguna Fresca
Salute
Laguna Fresca





 1
40.0
45.0
45.0
45.0
35.0
40.0


 2
40.0
45.0
45.0
45.0
35.0
40.0


 3
40.0
45.0
45.0
45.0
35.0
35.0


 4
40.0
45.0
40.0
45.0
30.0
45.0


 5
35.0
45.0
40.0
45.0
35.0
35.0


 6
35.0
45.0
45.0
45.0
35.0
45.0


 7
40.0
50.0
40.0
50.0
35.0
45.0


 8
40.0
50.0
40.0
50.0
35.0
45.0


 9
35.0
45.0
35.0
45.0
30.0
45.0


10
40.0
45.0
30.0
50.0
35.0
55.0


11
40.0
50.0
35.0
55.0
35.0
45.0


12
45.0
50.0
35.0
50.0
35.0
50.0


13
30.0
45.0
45.0
50.0
30.0
45.0


14
35.0
45.0
45.0
50.0
30.0
50.0


15
30.0
45.0
35.0
55.0
40.0
50.0


16
35.0
50.0
35.0
45.0
35.0
45.0


17
35.0
45.0
40.0
50.0
35.0
45.0


18
35.0
50.0
35.0
55.0
35.0
45.0


19
30.0
45.0
40.0
55.0
30.0
45.0


20
30.0
50.0
45.0
45.0
35.0
45.0


21
35.0
50.0
35.0
45.0
40.0
45.0


22
35.0
45.0
30.0
40.0
35.0
50.0


23
35.0
55.0
35.0
45.0
35.0
50.0


24
40.0
55.0
35.0
40.0
30.0
45.0


25
40.0
45.0
45.0
45.0
35.0
45.0


26
45.0
50.0
40.0
50.0
35.0
40.0


27
40.0
45.0
45.0
45.0
30.0
40.0


28
35.0
45.0
35.0
40.0
30.0
45.0


29
35.0
40.0
30.0
55.0
35.0
45.0


30
35.0
40.0
35.0
45.0
35.0
45.0


Average
36.8
46.8
38.7
47.5
34.0
44.8


stan dev
4.04E+00
3.59E+00
5.07E+00
4.50E+00
2.75E+00
4.25E+00


T test
1.94E−14

1.75E−09

6.41E−17


Probability %
100.0000

100.0000

100.0000


% Difference
−21.4

−18.6

−24.2


Confidence Int
0.0463
0.0411
0.0581
0.0515
0.0315
0.0487


Range of Var min*
36.79
46.79
38.61
47.45
33.97
44.78


Range of Var max*
36.88
46.87
38.72
47.55
34.03
44.88





*Range of variation among means of statistically significant differences at the 95% level using the confidence interval


[C] = mean +/− {SDXSE}













TABLE 2





Evaluation of head diameter of ‘Salute’ and most similar variety ‘Laguna Fresca’

















Trial No.











1
2
3









Location











Summer Year 8
Summer Year 8
Summer Year 8



King City, CA
King City, CA
King City, CA














Head diam (g)

Head diam (g)

Head diam (g)



Plant
Salute
Laguna Fresca
Salute
Laguna Fresca
Salute
Laguna Fresca





 1
132.0
145.0
135.0
150.0
135.0
150.0


 2
135.0
148.0
135.0
155.0
140.0
145.0


 3
130.0
145.0
135.0
155.0
140.0
145.0


 4
130.0
145.0
130.0
155.0
135.0
145.0


 5
135.0
148.0
125.0
154.0
145.0
150.0


 6
136.0
158.0
128.0
150.0
145.0
150.0


 7
138.0
152.0
130.0
150.0
145.0
145.0


 8
140.0
157.0
130.0
158.0
148.0
150.0


 9
135.0
150.0
136.0
156.0
145.0
155.0


10
135.0
150.0
138.0
155.0
143.0
155.0


11
135.0
152.0
140.0
150.0
140.0
145.0


12
135.0
155.0
145.0
150.0
135.0
145.0


13
135.0
150.0
140.0
155.0
135.0
150.0


14
135.0
145.0
135.0
150.0
136.0
150.0


15
138.0
142.0
140.0
160.0
130.0
150.0


16
136.0
130.0
135.0
168.0
140.0
145.0


17
130.0
154.0
135.0
155.0
135.0
140.0


18
130.0
150.0
140.0
154.0
140.0
140.0


19
125.0
150.0
145.0
150.0
145.0
145.0


20
130.0
155.0
130.0
150.0
130.0
145.0


21
135.0
155.0
135.0
155.0
130.0
150.0


22
120.0
145.0
135.0
155.0
135.0
145.0


23
125.0
145.0
130.0
140.0
140.0
140.0


24
123.0
148.0
138.0
145.0
135.0
145.0


25
135.0
148.0
140.0
150.0
140.0
140.0


26
135.0
145.0
140.0
145.0
135.0
145.0


27
130.0
145.0
145.0
145.0
135.0
150.0


28
130.0
150.0
130.0
140.0
150.0
145.0


29
134.0
150.0
135.0
145.0
140.0
140.0


30
132.0
150.0
140.0
150.0
135.0
140.0


Average
132.5
148.7
135.8
151.7
138.7
146.2


Stan dev
4.61E+00
5.39E+00
5.16E+00
5.79E+00
5.30E+00
4.29E+00


T test
3.46E−18

4.23E−16

1.54E−07



Probability %
100.00

100.00

100.00



% Difference
10.9

10.4

5.1



Confidence Int
0.0527
0.0618
0.0591
0.0663
0.0607
0.0491


Range of Var min*
132.41
148.67
135.77
151.60
138.67
146.12


Range of Var max*
132.52
148.80
135.89
151.73
138.79
146.22












Trial No.











4
5
6









Location











Summer Year 9
Summer Year 9
Summer Year 9



King City, CA
King City, CA
King City, CA














Head diam (g)

Head diam (g)

Head diam (g)



Plant
Salute
Laguna Fresca
Salute
Laguna Fresca
Salute
Laguna Fresca





 1
135.0
145.0
130.0
145.0
135.0
135.0


 2
130.0
150.0
130.0
145.0
130.0
145.0


 3
135.0
150.0
135.0
145.0
130.0
148.0


 4
132.0
150.0
140.0
150.0
130.0
148.0


 5
132.0
145.0
130.0
154.0
125.0
152.0


 6
130.0
150.0
135.0
145.0
125.0
135.0


 7
134.0
150.0
135.0
158.0
130.0
150.0


 8
140.0
145.0
140.0
158.0
130.0
158.0


 9
145.0
145.0
145.0
158.0
125.0
156.0


10
140.0
150.0
140.0
155.0
130.0
150.0


11
140.0
145.0
145.0
150.0
135.0
155.0


12
145.0
145.0
135.0
14.0
140.0
153.0


13
150.0
140.0
135.0
158.0
135.0
155.0


14
145.0
145.0
135.0
150.0
135.0
150.0


15
145.0
150.0
150.0
150.0
130.0
140.0


16
150.0
155.0
150.0
150.0
130.0
150.0


17
145.0
155.0
135.0
158.0
135.0
150.0


18
140.0
154.0
134.0
155.0
135.0
140.0


19
140.0
150.0
145.0
155.0
130.0
145.0


20
145.0
150.0
140.0
154.0
135.0
135.0


21
135.0
135.0
145.0
155.0
125.0
135.0


22
134.0
135.0
140.0
155.0
130.0
130.0


23
140.0
150.0
135.0
150.0
135.0
135.0


24
135.0
140.0
130.0
155.0
140.0
135.0


25
130.0
145.0
135.0
154.0
135.0
155.0


26
135.0
140.0
140.0
155.0
135.0
150.0


27
130.0
145.0
135.0
155.0
130.0
150.0


28
135.0
145.0
140.0
150.0
135.0
145.0


29
135.0
150.0
140.0
155.0
140.0
145.0


30
140.0
140.0
135.0
158.0
135.0
155.0


Average
138.2
146.5
138.0
148.3
132.3
146.2


Stan dev
5.96E+00
5.22E+00
5.53E+00
2.57E+01
4.30E+00
7.94E+00


T test
4.43E−07

3.55E−02

138E−11


Probability %
100.00

96.45

100.00


% Difference
5.6

7.0

9.5


Confidence Int
0.0683
0.0598
0.0633
0.2943
0.0493
0.0909


Range of Var min*
138.17
146.41
137.90
148.01
132.28
146.08


Range of Var max*
138.30
146.53
138.03
148.59
132.38
146.26





*Range of variation among means of statistically significant differences at the 95% level using the confidence interval


[C] = mean +/− {SDXSE}






‘Salute’ is a new and distinct variety of iceberg lettuce that most closely resembles the iceberg variety ‘Laguna Fresca’. ‘Salute’ is a Salinas type iceberg lettuce variety adapted to warmer lettuce production regions of the Salinas valley and the spring harvest period of the Yuma Ariz. production region. ‘Salute’ is a medium heading and large framed variety, with excellent heading characteristics, head shape, leaf texture and significantly improved resistance to tip burn and bolting.
‘Salute’ is earlier maturing, smaller heading, slower bolting and has better tip burn resistance than ‘Laguna Fresca’. ‘Salute’ is typically two to three days earlier maturing than ‘Laguna Fresca’, and has much smoother ribs and more consistent heading characteristics. Similar to ‘Laguna Fresca’, ‘Salute’ is adapted to the warmer production regions of the Salinas valley, but demonstrates a heavier leaf texture, improved heading characteristics, is slower bolting, and has excellent tip burn resistance. Unlike ‘Laguna Fresca’, ‘Salute’ is also adapted to the heat intense spring harvest slot in the desert south west production region. Under these extreme conditions, ‘Salute’ continues to demonstrate excellent heading characteristics, a large frame, low core, and tip burn resistance.
Although the foregoing invention has been described in some detail by way of illustration and examples for purposes of clarity and understanding, it will be obvious that certain modifications and alternative embodiments of the invention are contemplated which do not depart from the spirit and scope of the invention as defined by the foregoing teachings and appended claims.

## Claims

1. Lactuca sativa seed designated as ‘Salute’ having ATCC Accession Number PTA-13110.

2. A Lactuca sativa plant produced by growing the seed of claim 1.

3. A lettuce head isolated from the plant of claim 2.

4. A Lactuca sativa plant having all the physiological and morphological characteristics of the Lactuca sativa plant of claim 2.

5. An F1 hybrid Lactuca sativa plant having ‘Salute’ as a parent where ‘Salute’ is grown from the seed of claim 1.

6. Pollen of the plant of claim 2.

7. An ovule of the plant of claim 2.

8. Tissue culture of the plant of claim 2.

9. A method of selecting lettuce, comprising:
a) growing more than one plant from the seed of claim 1; and
b) selecting a plant from step a).