7/8/2014 ARS Project: DEVELOPMENT OF GENETIC MARKER SYSTEMS FOR USE IN RESEARCH ON THE QUANTITATI

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Research Project: DEVELOPMENT OF GENETIC MARKER

SYSTEMS FOR USE IN RESEARCH ON THE QUANTITATIVE Project Team
AND MOLECULAR GENETICS OF PACIFIC OYSTERS Camara, Mark
Chris Langdon - Marine
Location: Forage Seed and Cereal Research Biologist
2007 Annual Report Project Annual Reports
FY 2008
1a.Objectives (from AD-416) FY 2007
The objective of this cooperative research project is to elucidate the
genetic architecture underlying economically important traits in FY 2006
cultured Pacific oysters (Crassistrea gigas) and to use this information FY 2005
to develop marker-assisted selection technology that enhances
broodstock development with improved characteristics such as growth FY 2004

rate, reproduction, survival, disease resistance, and product quality.
Information will be utilized from existing archived samples and

genetic maps and new information will be contributed to mapping Related National Programs
databases by mapping quantitative trait loci (QTL) for traits of Aquaculture (106)

economic importance. The development of these tools will allow the
integration of marker-assisted selection with traditional breeding
approaches to provide the shellfish industry with improved genetic
stock. The program is dependent upon coordination with the Molluscan
Broodstock Program (MBP) at the Hatfield Marine Science Center
(HMSC), an administrative unit of Oregon State University.

1b.Approach (from AD-416)

The levels of genetic (co)variation available in existing and potential
shellfish germ plasm resources for economically important phenotypic
characters as well as the number of loci contributing to these traits
and the nature of their effects (genetic architecture) will be examined
through a combination of quantitative and molecular genetic
approaches. These include reconstructing and verifying pedigrees of
broodstock animals using molecular markers, traditional quantitative
genetic variance partitioning among families of animals of known
relatedness and quantitative trait locus mapping. The patterns of
available genetic (co)variation and genetic architecture are critical
information for the design and execution of effective selection
protocols for genetic improvement. Identifying QTL contributing to
economically important traits will allow for the development of marker-
assisted selection strategies that will improve the efficiency of
selective breeding by allowing direct selection of favorable genotypes
and by reducing the interval between spawning and selection and the
need to maintain undesirable genotypes through the entire life cycle.
Documents NFCA with Oregon State University. Formerly 5358-31000-
001-01N. 04/2005.

3.Progress Report

This report serves to document research conducted under Non-Funded
Cooperative Agreement 5358-31000-002-01N between USDA ARS and
Oregon State University, Hatfield Marine Science Center. Additional
details of this research can be found in the report for the in-house
associated project 5358-31000-002-00D "Genetic Characterization and
Improvement of Shellfish Breeding Stocks for the Pacific Northwest".
This project focuses on the development of genetically improved
shellfish stocks through selective breeding using both quantitative and
molecular genetic approaches. In the past year, we have collected new
germplasm of both Pacific and Kumamoto oysters from their native
range in the Ariake Sea in Japan and produced the first captive
generation under strict quarantine conditions at the Hatfield Marine
Science Center. We have also conducted extensive disease testing
and hybridization experiments between these two species. We also
produced 24 F2 mapping families of Pacific oysters from last year's F1
families which will be used to identify quantitative trait lock for
economically important traits, conducted a large scale analysis of
population structure in native Olympia oyster populations in
Washington, Oregon, and California, and produced a software
package that identifies the most informative genetic markers for

https:/|web.archive.or g/wweb/20090515091448/http:/Amww.ars.usda.goviresearchiprojects/projects.hm?ACCN_NO=408065&showpars=true8fy=2007

IVE AND MOLECULAR GENETI...


7/8/2014 ARS Project: DEVELOPMENT OF GENETIC MARKER SYSTEMS FOR USE IN RESEARCH ON THE QUANTITATIVE AND MOLECULAR GENETI...

pedigree re-construction from mixtures of families.

ADODR monitored the research progress and kept the cooperator
informed

Last Modified: 05/14/2009
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