7/8/2014 ARS Project: DEVELOPMENT OF GENETIC MARKER SYSTEMS FOR USE IN RESEARCH ON THE QUANTITATIVE AND MOLECULAR GENET.

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Research Project: DEVELOPMENT OF GENETIC MARKER
SYSTEMS FOR USE IN RESEARCH ON THE QUANTITATIVE
AND MOLECULAR GENETICS OF PACIFIC OYSTERS

Location: Forage Seed and Cereal Research
2008 Annual Report

1a.Objectives (from AD-416)
The objective of this cooperative research project is to elucidate the
genetic architecture underlying economically important traits in
cultured Pacific oysters (Crassistrea gigas) and to use this information
to develop marker-assisted selection technology that enhances
broodstock development with improved characteristics such as growth
rate, reproduction, survival, disease resistance, and product quality.
Information will be utilized from existing archived samples and
genetic maps and new information will be contributed to mapping
databases by mapping quantitative trait loci (QTL) for traits of
economic importance. The development of these tools will allow the
integration of marker-assisted selection with traditional breeding
approaches to provide the shellfish industry with improved genetic
stock. The program is dependent upon coordination with the Molluscan
Broodstock Program (MBP) at the Hatfield Marine Science Center
(HMSC), an administrative unit of Oregon State University.

1b.Approach (from AD-416)
The levels of genetic (co)variation available in existing and potential
shellfish germ plasm resources for economically important phenotypic
characters as well as the number of loci contributing to these traits
and the nature of their effects (genetic architecture) will be examined
through a combination of quantitative and molecular genetic
approaches. These include reconstructing and verifying pedigrees of
broodstock animals using molecular markers, traditional quantitative
genetic variance partitioning among families of animals of known
relatedness and quantitative trait locus mapping. The patterns of
available genetic (co)variation and genetic architecture are critical
information for the design and execution of effective selection
protocols for genetic improvement. Identifying QTL contributing to
economically important traits will allow for the development of marker-
assisted selection strategies that will improve the efficiency of
selective breeding by allowing direct selection of favorable genotypes
and by reducing the interval between spawning and selection and the
need to maintain undesirable genotypes through the entire life cycle.
Documents NFCA with Oregon State University. Formerly 5358-31000-
001-01N. 04/2005.

3.Progress Report
This project focuses on the development of genetically improved
shellfish stocks through selective breeding using both quantitative and
molecular genetic approaches. In the past year, we have focused on
analyses of transcription-level responses of Pacific oysters to heat
stress and bacterial infection, mapping quantitative trait loci for growth
and survival, and evaluating strategies to increase the efficacy of
selective breeding efforts by using molecular markers to reconstruct
pedigree information rather than rearing families separately. We are
also continuing to propagate recently-collected novel Kumamoto and
Pacific oyster germplasm under quarantine conditions for eventual
release to the oyster culture industry.

The ADODR monitored the cooperator’s performance on this

extramural agreement through site visits, meetings, conference calls,
email correspondence and review of data.

Last Modified: 05/14/2009

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Project Team
Camara, Mark

_ Chris Langdon - Marine
° Biologist

Project Annual Reports
o FY 2008
o FY 2007
o FY 2006
o FY 2005
o FY 2004

Related National Programs
o Aquaculture (106)


7/8/2014 ARS Project: DEVELOPMENT OF GENETIC MARKER SYSTEMS FOR USE IN RESEARCH ON THE QUANTITATIVE AND MOLECULAR GENETI...

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