13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J12090146

Project Name: Somerville Waste Water

Lab Contact: Jason C Perkins

Date: 10/1/2012

Customer Address: 253 Plant Allen Road

Customer Name(s): BillK-RonL--RobnJ-DonS

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality 

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data 

included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the 

subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have 

been qualified by Duke Energy to perform these analyses except where noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Page 1 of 42
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2012019595 ALLEN 05-Sep-12 2:03 PM Chris Williams FGD Purge Eff 

2012019596 ALLEN 05-Sep-12 1:34 PM Chris Williams EQ Tank Eff 

2012019597 ALLEN 05-Sep-12 1:37 PM Chris Williams BioReactor 1 Inf 

2012019598 ALLEN 05-Sep-12 1:37 PM Chris Williams BioReactor 1 Inf BLANK 

2012019599 ALLEN 05-Sep-12 1:45 PM Chris Williams BioReactor 2 Inf 

2012019600 ALLEN 05-Sep-12 1:45 PM Chris Williams BioReactor 2 Inf BLANK 

2012019601 ALLEN 05-Sep-12 1:55 PM Chris Williams BioReactor 2 Eff 

2012019602 ALLEN 05-Sep-12 1:55 PM Chris Williams BioReactor 2 Eff BLANK 

2012019603 ALLEN 05-Sep-12 2:15 PM Chris Williams Filter Blk 

9 Total Samples

Page 2 of 42
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: DataBase Administrator Date: 10/1/2012

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019595

Collection Date: 05-Sep-12 2:03 PM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 380 mg/L 5 50 EPA 300.0 9/17/2012 9:37:00 PM JAHERMA

Chloride 1600 mg/L 50 500 EPA 300.0 9/17/2012 9:37:00 PM JAHERMA

Sulfate 1500 mg/L 50 500 EPA 300.0 9/17/2012 9:37:00 PM JAHERMA

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 19.2 ug/L 2.5 50 EPA 245.1 9/13/2012 2:38:28 PM AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/12/2012 10:16:00 AM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 104 mg/L 0.5 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Calcium (Ca) 4220 mg/L 0.1 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Iron (Fe) 123 mg/L 0.1 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Lithium (Li) 0.178 mg/L 0.05 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Magnesium (Mg) 505 mg/L 0.05 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Manganese (Mn) 5.31 mg/L 0.05 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Potassium (K) 34.6 mg/L 1 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

Sodium (Na) 25.6 mg/L 0.5 10 EPA 200.7 9/26/2012 11:59:00 AM DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 1760 ug/L 10 10 EPA 200.8 9/18/2012 3:05:00 PM KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 233 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Chromium (Cr) 228 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Copper (Cu) 193 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Nickel (Ni) 261 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Selenium (Se) 2940 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

Zinc (Zn) 366 ug/L 10 10 EPA 200.8 9/25/2012 11:42:00 AM KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 8600 mg/L 200 1 SM2540C 9/12/2012 3:51:00 PM SWILLI3

TOTAL SUSPENDED SOLIDS

TSS 3700 mg/L 250 1 SM2540D 9/13/2012 1:55:00 PM SWILLI3

Page 4 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019595

Collection Date: 05-Sep-12 2:03 PM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

2012019596

Collection Date: 05-Sep-12 1:34 PM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 23.7 ug/L 2.5 50 EPA 245.1 9/13/2012 2:40:51 PM AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/12/2012 10:19:00 AM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 113 mg/L 0.5 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Calcium (Ca) 1950 mg/L 0.1 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Iron (Fe) 68.4 mg/L 0.1 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Lithium (Li) 0.132 mg/L 0.05 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Magnesium (Mg) 525 mg/L 0.05 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Manganese (Mn) 2.51 mg/L 0.05 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Potassium (K) 27.2 mg/L 1 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

Sodium (Na) 40.4 mg/L 0.5 10 EPA 200.7 9/26/2012 12:03:00 PM DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 2090 ug/L 10 10 EPA 200.8 9/18/2012 3:08:00 PM KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 120 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Chromium (Cr) 144 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Copper (Cu) 120 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Nickel (Ni) 186 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Selenium (Se) 2760 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

Zinc (Zn) 245 ug/L 10 10 EPA 200.8 9/25/2012 11:45:00 AM KRICHAR

2012019597

Collection Date: 05-Sep-12 1:37 PM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

Page 5 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019597

Collection Date: 05-Sep-12 1:37 PM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/12/2012 10:23:00 AM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 105 mg/L 0.5 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Calcium (Ca) 1540 mg/L 0.1 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Iron (Fe) < 0.1 mg/L 0.1 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Lithium (Li) 0.062 mg/L 0.05 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Magnesium (Mg) 414 mg/L 0.05 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Potassium (K) 15.2 mg/L 1 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

Sodium (Na) 77.4 mg/L 0.5 10 EPA 200.7 9/26/2012 12:07:00 PM DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 2660 ug/L 10 10 EPA 200.8 9/18/2012 3:11:00 PM KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Chromium (Cr) 17.1 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Selenium (Se) 2940 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:48:00 AM KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2012019598

Collection Date: 05-Sep-12 1:37 PM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

Page 6 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019598

Collection Date: 05-Sep-12 1:37 PM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2012019599

Collection Date: 05-Sep-12 1:45 PM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/12/2012 10:26:00 AM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 109 mg/L 0.5 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Calcium (Ca) 1570 mg/L 0.1 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Iron (Fe) 0.132 mg/L 0.1 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Lithium (Li) 0.065 mg/L 0.05 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Magnesium (Mg) 422 mg/L 0.05 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Potassium (K) 21.7 mg/L 1 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

Sodium (Na) 78.0 mg/L 0.5 10 EPA 200.7 9/26/2012 12:11:00 PM DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 1440 ug/L 10 10 EPA 200.8 9/18/2012 3:15:00 PM KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Selenium (Se) 1520 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 9/25/2012 11:51:00 AM KRICHAR

Page 7 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019599

Collection Date: 05-Sep-12 1:45 PM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2012019600

Collection Date: 05-Sep-12 1:45 PM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2012019601

Collection Date: 05-Sep-12 1:55 PM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 480 mg/L 5 50 EPA 300.0 9/17/2012 9:55:00 PM JAHERMA

Chloride 2400 mg/L 50 500 EPA 300.0 9/17/2012 9:55:00 PM JAHERMA

Sulfate 1700 mg/L 50 500 EPA 300.0 9/17/2012 9:55:00 PM JAHERMA

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/12/2012 10:30:00 AM MHH7131

Page 8 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019601

Collection Date: 05-Sep-12 1:55 PM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 137 mg/L 0.5 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Calcium (Ca) 1530 mg/L 0.1 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Iron (Fe) 0.892 mg/L 0.1 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Lithium (Li) 0.067 mg/L 0.05 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Magnesium (Mg) 480 mg/L 0.05 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Potassium (K) 29.2 mg/L 1 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

Sodium (Na) 107 mg/L 0.5 10 EPA 200.7 9/26/2012 12:15:00 PM DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 14.5 ug/L 5 5 EPA 200.8 9/18/2012 3:18:00 PM KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Cadmium (Cd) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Chromium (Cr) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Copper (Cu) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Nickel (Ni) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Selenium (Se) 11.9 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Silver (Ag) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

Zinc (Zn) < 5 ug/L 5 5 EPA 200.8 9/25/2012 11:54:00 AM KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2012019602

Collection Date: 05-Sep-12 1:55 PM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

Page 9 of 42
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12090146

2012019603

Collection Date: 05-Sep-12 2:15 PM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.005 mg/L 0.005 1 EPA 200.7 9/12/2012 10:33:00 AM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 3.16 ug/L 1 1 EPA 200.8 9/18/2012 2:19:00 PM KRICHAR

Page 10 of 42
 

September 21, 2012 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen Shay/MillerCreek (LIMS#J12090146) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation analysis on September 11, 2012. The samples were received in a sealed cooler at

-0.4°C on September 12, 2012. Selenium speciation analysis was performed via ion 

chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICPCRC-MS). Any issues associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

 

Page 11 of 42
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen Shay/MillerCreek (LIMS#J12090146) 

September 21, 2012 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on September 11, 2012. The 

samples were received on September 12, 2012 in a sealed container at -0.4°C. 

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and were designated discrete sample identifiers. An aliquot of 

each sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored 

cryofreezer (maintained at a temperature of -80oC) until selenium speciation analysis could 

be performed via ion chromatography inductively coupled plasma collision reaction cell 

mass spectrometry (IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed 

autosampler vial. No further sample preparation was performed as any chemical alteration of 

a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

each analytical day. All calibration curves, associated with each species of interest, are 

Page 12 of 42
standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on September 21, 2012. An aliquot of each

sample is injected onto an anion exchange column and mobilized by a basic (pH > 7) 

gradient. The eluting selenium species are then introduced into a radio frequency (RF) 

plasma where energy-transfer processes cause desolvation, atomization, and ionization. The 

ions are extracted from the plasma through a differentially-pumped vacuum interface and 

travel through a pressurized chamber (CRC) containing a reaction gas which preferentially 

reacts with interfering ions of the same target mass to charge ratios (m/z). A solid-state 

detector detects ions transmitted through the mass analyzer and the resulting current is 

processed by a data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

other quality control parameters associated with the samples were within acceptance limits.

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Page 13 of 42
If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 14 of 42
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff 12.7 1580 3.4 ND (<0.92) ND (<0.92) 0.0 (0)

BioReactor 1 Inf 1.81 2510 0.33 0.79 ND (<0.23) 0.0 (0)

BioReactor 2 Inf 203 842 0.49 0.70 0.37 0.0 (0)

BioReactor 2 Eff 1.80 ND (<0.12) ND (<0.31) ND (<0.23) ND (<0.23) 0.0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

n = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

Date: September 21, 2012

LIMS #J12090146

Page 15 of 42
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0010 0.26 1.0

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.0005 0.12 0.47

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.0012 0.31 1.2

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0009 0.23 0.92

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.0009 0.23 0.92

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 9.30 97.2

Se(VI) LCS 9.48 8.94 94.3

SeCN LCS 8.92 8.60 96.4

MeSe(IV) LCS 6.47 6.07 93.9

SeMe LCS 9.32 8.67 93.1

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

LIMS #J12090146

Date: September 21, 2012

Report Generated by: Russell Gerads

Page 16 of 42
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) Batch QC 8.3 7.4 7.8 11.9

Se(VI) Batch QC 3068 3106 3087 1.2

SeCN Batch QC ND (<1.2) ND (<1.2) NC NC

MeSe(IV) Batch QC ND (<0.92) ND (<0.92) NC NC

SeMe Batch QC ND (<0.92) ND (<0.92) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) Batch QC 5560 5668 101.8 5560 5668 101.8 0.0

Se(VI) Batch QC 5045 8168 100.7 5045 8145 100.3 0.3

SeCN Batch QC 4575 4437 97.0 4575 4452 97.3 0.4

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

LIMS #J12090146

Date: September 21, 2012

Report Generated by: Russell Gerads

Page 17 of 42
Page 18 of 42
3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com

September 28, 2012

Duke Energy

ATTN: Jay Perkins

Scientific Support-Laboratory

13339 Hagers Ferry Road

Huntersville NC 28078

jcperkins@duke-energy.com

labcustomer@duke-energy.com

RE: Project DUK-HV1201 Client Project: J1209014

Dear Mr. Perkins,

On September 12, 2012, Brooks Rand Labs (BRL) received three (3) wastewater samples and

three (3) corresponding field blanks. An aliquot was removed from each sample bottle and 

filtered into a separate container designed for dissolved mercury (Hg) analysis. The sample 

volume from the original container was logged-in for total Hg analysis. All samples were 

received, prepared, analyzed, and stored according to BRL SOPs and EPA methodology.

Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received

outside of the non-regulatory requirement holding time and were qualified H. 

The results were blank-corrected as described in the calculations section of the relevant SOP

and may have been evaluated using reporting limits that have been adjusted to account for 

sample aliquot size. Please refer to the Sample Results page for sample-specific MDLs, MRLs, 

and other details. 

In sequences 1200724 and 1200736, the results of continuing calibration blank –CCB1 were 

greater than the low calibration; however, no client samples were bracketed by the analysis of –

CCB1 and all other CCBs results were low. The somewhat elevated –CCB1s were likely 

attributed to carryover from the previous analysis of the independent calibration verification 

standard -ICV1.

The continuing calibration verification standard –CCV4, analyzed in sequence 1200724, 

recovered at 123%- above the acceptance criteria range. No client samples from this work order 

were bracketed. 

Aside from concentration qualifiers, all data was reported without further qualification and all

associated quality control sample results met the acceptance criteria.

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. 

Page 19 of 42
Please feel free to contact me if you have any questions regarding this report.

Sincerely,

 

Tiffany Stilwater

Project Manager

tiffany@brooksrand.com

Page 20 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic 

Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 21 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1237018-01 Influent Sample 09/05/2012 09/12/2012

BioReactor 1 Inf 1237018-02 Influent Sample 09/05/2012 09/12/2012

BioReactor 1 Inf Hg Blk 1237018-03 DIW Field Blank 09/05/2012 09/12/2012

BioReactor 1 Inf Hg Blk 1237018-04 DIW Field Blank 09/05/2012 09/12/2012

BioReactor 2 Inf 1237018-05 Influent Sample 09/05/2012 09/12/2012

BioReactor 2 Inf 1237018-06 Influent Sample 09/05/2012 09/12/2012

BioReactor 2 Inf Hg Blk 1237018-07 DIW Field Blank 09/05/2012 09/12/2012

BioReactor 2 Inf Hg Blk 1237018-08 DIW Field Blank 09/05/2012 09/12/2012

BioReactor 2 Eff 1237018-09 Effluent Sample 09/05/2012 09/12/2012

BioReactor 2 Eff 1237018-10 Effluent Sample 09/05/2012 09/12/2012

BioReactor 2 Eff Hg BLk 1237018-11 DIW Field Blank 09/05/2012 09/12/2012

BioReactor 2 Eff Hg BLk 1237018-12 DIW Field Blank 09/05/2012 09/12/2012

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 09/18/2012 09/19/2012 B121708 1200724

Hg Water EPA 1631 09/18/2012 09/24/2012 B121708 1200736

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 22 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Basis Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1237018-01 Hg Influent T 2570 12.6 33.7 ng/L B121708 1200724

1237018-02 Hg Influent D 2230 H 7.58 20.2 ng/L B121708 1200724

BioReactor 1 Inf Hg Blk

1237018-03 Hg DIW T 0.89 0.15 0.39 ng/L B121708 1200724

1237018-04 Hg DIW D 0.15 H, U 0.15 0.39 ng/L B121708 1200724

BioReactor 2 Eff

1237018-09 Hg Effluent T 26.0 0.19 0.49 ng/L B121708 1200736

1237018-10 Hg Effluent D 14.1 H 0.15 0.40 ng/L B121708 1200736

BioReactor 2 Eff Hg BLk

1237018-11 Hg DIW T 0.21 B 0.15 0.39 ng/L B121708 1200724

1237018-12 Hg DIW D 0.15 H, U 0.15 0.39 ng/L B121708 1200724

BioReactor 2 Inf

1237018-05 Hg Influent T 665 3.83 10.2 ng/L B121708 1200736

1237018-06 Hg Influent D 29.2 H 0.15 0.41 ng/L B121708 1200736

BioReactor 2 Inf Hg Blk

1237018-07 Hg DIW T 4.13 0.15 0.41 ng/L B121708 1200724

1237018-08 Hg DIW D 0.23 H, B 0.15 0.39 ng/L B121708 1200724

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 23 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B121708

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B121708-SRM1 Certified Reference Material (1237042, NIST 1641d 1000x dilution)

Hg 62.72 68.70 ng/L 110% 85-115

B121708-SRM2 Certified Reference Material (1237042, NIST 1641d 1000x dilution)

Hg 62.72 61.78 ng/L 99% 85-115

B121708-DUP4 Duplicate (1237018-05)

Hg 664.9 660.8 ng/L 240.6%

B121708-MS4 Matrix Spike (1237018-05)

Hg 664.9 2296 2938 ng/L 99% 71-125

B121708-MSD4 Matrix Spike Duplicate (1237018-05)

Hg 664.9 2296 2921 ng/L 98% 71-125 240.6%

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 24 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Method Blanks & Reporting Limits

Batch: B121708

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B121708-BLK1 0.27 ng/L

B121708-BLK2 0.13 ng/L

B121708-BLK3 0.12 ng/L

B121708-BLK4 0.14 ng/L

Average: 0.17 Standard Deviation: 0.07 MDL: 0.16

Limit: 0.50 Limit: 0.10 MRL: 0.42

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 25 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200724

Instrument: THG-05 Method: EPA 1631

Date: 09/19/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200724-IBL1 0.55 pg of Hg

1200724-IBL2 0.59 pg of Hg

1200724-IBL3 0.85 pg of Hg

1200724-IBL4 1.49 pg of Hg

1200724-CAL1 10.00 8.44 pg of Hg 84%

1200724-CAL2 25.00 24.04 pg of Hg 96%

1200724-CAL3 100.0 100.6 pg of Hg 101%

1200724-CAL4 500.0 518.9 pg of Hg 104%

1200724-CAL5 2500 2773 pg of Hg 111%

1200724-CAL6 10000 10920 pg of Hg 109%

1200724-ICV1 1568 1718 pg of Hg 110% 85-115

1200724-CCB1 14.4 pg of Hg

1200724-CCV1 500.0 551.6 pg of Hg 110% 77-123

1200724-CCB2 6.70 pg of Hg

1200724-CCB3 4.20 pg of Hg

1200724-CCB4 4.65 pg of Hg

1200724-CCV2 500.0 551.2 pg of Hg 110% 77-123

1200724-CCB5 3.76 pg of Hg

1200724-CCV3 500.0 589.9 pg of Hg 118% 77-123

1200724-CCB6 9.75 pg of Hg

1200724-CCV4 500.0 615.6 pg of Hg 123% 77-123

1200724-CCB7 6.91 pg of Hg

1200724-CCV5 500.0 609.3 pg of Hg 122% 77-123

1200724-CCB8 6.61 pg of Hg

1200724-CCV6 500.0 603.8 pg of Hg 121% 77-123

1200724-CCB9 4.54 pg of Hg

1200724-CCV7 500.0 581.0 pg of Hg 116% 77-123

1200724-CCBA 4.28 pg of Hg

1200724-CCV8 500.0 564.1 pg of Hg 113% 77-123

1200724-CCBB 3.89 pg of Hg

1200724-CCV9 500.0 581.3 pg of Hg 116% 77-123

1200724-CCBC 6.47 pg of Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 26 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200736

Instrument: THG-05 Method: EPA 1631

Date: 09/24/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200736-IBL1 1.30 pg of Hg

1200736-IBL2 1.05 pg of Hg

1200736-IBL3 2.65 pg of Hg

1200736-IBL4 2.88 pg of Hg

1200736-CAL1 10.00 10.53 pg of Hg 105%

1200736-CAL2 25.00 24.49 pg of Hg 98%

1200736-CAL3 100.0 96.17 pg of Hg 96%

1200736-CAL4 500.0 493.0 pg of Hg 99%

1200736-CAL5 2500 2617 pg of Hg 105%

1200736-CAL6 10000 9804 pg of Hg 98%

1200736-ICV1 1568 1545 pg of Hg 99% 85-115

1200736-CCB1 10.4 pg of Hg

1200736-CCV1 500.0 505.3 pg of Hg 101% 77-123

1200736-CCB2 5.42 pg of Hg

1200736-CCB3 4.57 pg of Hg

1200736-CCB4 5.91 pg of Hg

1200736-CCV2 500.0 501.5 pg of Hg 100% 77-123

1200736-CCB5 5.23 pg of Hg

1200736-CCV3 500.0 499.5 pg of Hg 100% 77-123

1200736-CCB6 4.64 pg of Hg

1200736-CCV4 500.0 504.1 pg of Hg 101% 77-123

1200736-CCB7 4.52 pg of Hg

1200736-CCV5 500.0 476.3 pg of Hg 95% 77-123

1200736-CCB8 4.57 pg of Hg

1200736-CCV6 500.0 471.7 pg of Hg 94% 77-123

1200736-CCB9 4.42 pg of Hg

1200736-CCV7 500.0 477.2 pg of Hg 95% 77-123

1200736-CCBA 4.55 pg of Hg

1200736-CCV8 500.0 488.6 pg of Hg 98% 77-123

1200736-CCBB 6.71 pg of Hg

1200736-CCV9 500.0 488.4 pg of Hg 98% 77-123

1200736-CCBC 7.59 pg of Hg

1200736-CCVA 500.0 500.5 pg of Hg 100% 77-123

1200736-CCBD 6.49 pg of Hg

1200736-CCVB 500.0 493.7 pg of Hg 99% 77-123

1200736-CCBE 9.03 pg of Hg

1200736-CCVC 500.0 485.7 pg of Hg 97% 77-123

1200736-CCBF 6.18 pg of Hg

1200736-CCVD 500.0 484.7 pg of Hg 97% 77-123

1200736-CCBG 4.09 pg of Hg

1200736-CCVE 500.0 484.5 pg of Hg 97% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 27 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200736

Instrument: THG-05 Method: EPA 1631

Date: 09/24/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200736-CCBH 3.74 pg of Hg

1200736-CCVF 500.0 488.8 pg of Hg 98% 77-123

1200736-CCBI 5.50 pg of Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 28 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1237018-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 09/12/2012

Collected: 09/05/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1237018-02 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 09/12/2012

Collected: 09/05/2012

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1237018-03 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 09/12/2012

Collected: 09/05/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1237018-04 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 09/12/2012

Collected: 09/05/2012

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1237018-05 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 09/12/2012

Collected: 09/05/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1237018-06 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 09/12/2012

Collected: 09/05/2012

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 29 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1237018-07 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 09/12/2012

Collected: 09/05/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1237018-08 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 09/12/2012

Collected: 09/05/2012

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1237018-09 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 09/12/2012

Collected: 09/05/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1237018-10 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 09/12/2012

Collected: 09/05/2012

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1237018-11 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg BLk Sample Type: Field Blank Received: 09/12/2012

Collected: 09/05/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1237018-12 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg BLk Sample Type: Field Blank Received: 09/12/2012

Collected: 09/05/2012

Comments: Qualify H

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 30 of 42
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Shipping Containers

Cooler

Tracking No: 535305194038 via FedEx

Temperature: 0.4 °C

Coolant Type: Ice

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: September 12, 2012 9:00

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 31 of 42
Page 32 of 42
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

09/14/2012

Duke Energy Corporation (04)

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: Allen Shay/Miller Creek

Project No.: J12090146

Lab Submittal Date: 09/11/2012

Prism Work Order: 2090190

VA Certification No. 1287

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 9

Page 33 of 42
Sample Receipt Summary

09/14/2012

Prism Work Order: 2090190

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2012019595/FGD Purge Eff 2090190-01 Water 09/05/12 09/11/12

2012019597/BioReactor 1 Inf 2090190-02 Water 09/05/12 09/11/12

2012019599/BioReactor 2 Inf 2090190-03 Water 09/05/12 09/11/12

2012019601/BioReactor 2 Eff 2090190-04 Water 09/05/12 09/11/12

Samples received in good condition at 0.4 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 9

Page 34 of 42
Laboratory Report

09/14/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2090190-01

Prism Work Order: 2090190

Time Collected: 09/05/12 14:03

Time Submitted: 09/11/12 16:10

2012019595/FGD Purge Eff

J12090146

General Chemistry Parameters

pH 7.1 HT pH Units 1 *SM4500-H B 9/12/12 11:00 JAB P2I0176

Total Alkalinity 48 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 13:57 JAB P2I0177

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0178

Bicarbonate Alkalinity 48 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0179

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 9

Page 35 of 42
Laboratory Report

09/14/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2090190-02

Prism Work Order: 2090190

Time Collected: 09/05/12 13:37

Time Submitted: 09/11/12 16:10

2012019597/BioReactor 1 Inf

J12090146

General Chemistry Parameters

pH 6.8 HT pH Units 1 *SM4500-H B 9/12/12 11:00 JAB P2I0176

Total Alkalinity 24 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 13:57 JAB P2I0177

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0178

Bicarbonate Alkalinity 24 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0179

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 9

Page 36 of 42
Laboratory Report

09/14/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2090190-03

Prism Work Order: 2090190

Time Collected: 09/05/12 13:45

Time Submitted: 09/11/12 16:10

2012019599/BioReactor 2 Inf

J12090146

General Chemistry Parameters

pH 7.2 HT pH Units 1 *SM4500-H B 9/12/12 11:00 JAB P2I0176

Total Alkalinity 180 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 13:57 JAB P2I0177

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0178

Bicarbonate Alkalinity 180 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0179

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 9

Page 37 of 42
Laboratory Report

09/14/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2090190-04

Prism Work Order: 2090190

Time Collected: 09/05/12 13:55

Time Submitted: 09/11/12 16:10

2012019601/BioReactor 2 Eff

J12090146

General Chemistry Parameters

pH 6.8 HT pH Units 1 *SM4500-H B 9/12/12 11:00 JAB P2I0176

Total Alkalinity 120 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 13:57 JAB P2I0177

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0178

Bicarbonate Alkalinity 120 mg/L 5.0 0.66 1 *SM2320 B 9/12/12 11:00 JAB P2I0179

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 9

Page 38 of 42
9/14/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12090146

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2090190

Time Submitted: 9/11/2012 4:10:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2I0176 - NO PREP

LCS (P2I0176-BS1) Prepared & Analyzed: 09/12/12 

pH 6.81 pH Units 6.860 99 99-101

Batch P2I0177 - NO PREP

Blank (P2I0177-BLK1) Prepared & Analyzed: 09/12/12 

Total Alkalinity BRL 5.0 mg/L

LCS (P2I0177-BS1) Prepared & Analyzed: 09/12/12 

Total Alkalinity 243 5.0 mg/L 250.0 97 90-110

LCS Dup (P2I0177-BSD1) Prepared & Analyzed: 09/12/12 

Total Alkalinity 250 5.0 mg/L 250.0 100 90-110 3 200

Batch P2I0178 - NO PREP

Blank (P2I0178-BLK1) Prepared & Analyzed: 09/12/12 

Carbonate Alkalinity BRL 5.0 mg/L

LCS (P2I0178-BS1) Prepared & Analyzed: 09/12/12 

Carbonate Alkalinity 243 5.0 mg/L 90-110

LCS Dup (P2I0178-BSD1) Prepared & Analyzed: 09/12/12 

Carbonate Alkalinity 250 5.0 mg/L 90-110 3 200

Batch P2I0179 - NO PREP

Blank (P2I0179-BLK1) Prepared & Analyzed: 09/12/12 

Bicarbonate Alkalinity BRL 5.0 mg/L

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 9

Page 39 of 42
9/14/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12090146

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2090190

Time Submitted: 9/11/2012 4:10:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2I0179 - NO PREP

LCS (P2I0179-BS1) Prepared & Analyzed: 09/12/12 

Bicarbonate Alkalinity 243 5.0 mg/L 250.0 97 90-110

LCS Dup (P2I0179-BSD1) Prepared & Analyzed: 09/12/12 

Bicarbonate Alkalinity 250 5.0 mg/L 250.0 100 90-110 3 200

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 8 of 9

Page 40 of 42
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