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Submission Report

Submission Information

Submission ID :

25349470

Submission Name : Quaternary ammonium compounds, benzyl-C12-16-

alkyldimethyl, chlorides

AR Number : 

Chemical Information

CAS Number :

68424-85-1

Ninth Collective Index Name : Quaternary ammonium compounds, benzyl-C12-

16-alkyldimethyl, chlorides

Submitter Information

Submitter's Name : EPA

Address : 

City : Washington

State : DC

ZIP/Postal Code: 

Country: 

Type of Submitter : Company

Technical Point of Contact Information

Technical Point of Contact : 

Title : 

Phone Number : 

Fax Number : 

E-Mail Address : 

Additional Technical Point of Contact Information

Technical Point of Contact : 

Title : 

Phone Number : 

Fax Number : 

E-Mail Address : 

Sponsor Information

Sponsor's Name : EPA

Address : 

City : 

State : 

ZIP/Postal Code : 

Country : 

Phone Number : 
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Fax Number : 

E-Mail Address : 

Type of Sponsor : Company

Technical Point of Contact Information

Technical Point of Contact : 

Title : 

Phone Number : 

Fax Number : 

E-Mail Address : 

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 80% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

EPA OPPTS 850.1075

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Cyprinodon variegatus

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Saltwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.86 mg/L Mortality Unknown
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Results Remarks: LC50 = 0.86 mg/L (a.i.); NOAEC = 0.68 mg/L (a.i.)

Highly toxic; categorized as highly toxic to fish

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 424795-02. Sved, D.W., J.P. Swigert, and G.J. Smith. 1992. A 96-Hour Static

Renewal Acute 

Toxicity Test with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in the Sheepshead

Minno 

(Cyprinodon variegatus). Project No. 350A-102. Unpublished data. Conducted by

Wildlife 

International Ltd. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers

Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 30% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Species: Oncorhynchus mykiss

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

96 Hours

NOEC: = 0.619 mg/L Unknown

LOEC Exposure

Duration:

LOEC:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.923 mg/L Mortality Unknown

Results Remarks: Highly toxic; categorized as highly toxic to fish

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 419472-02. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 96-

Hour Toxicity 

Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Rainbow Trout. Study No.

SC890051. 

Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint

Venture/Chemical 

Specialties Manufacturers Association. 

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

ADBAC, 80% AI
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Yes

Species: Pimephales promelas

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Deviations from guideline include additions of 20 mg/L humic acid to dilution water

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

96 Hours

NOEC: = 0.99 mg/L Unknown

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 1.4 mg/L Mortality Unknown

Results Remarks: Moderately toxic; categorized as moderately toxic to fish

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 437401-01. Sword, M.C. and L. Stuerman. 1993. Static-Renewal Acute Toxicity

of ADBAC to 

Fathead Minnow (Pimephales promelas) in Dilution Water Amended with 20 mg/L Humic

Acid. Study 

No. 41235. Unpublished data. Conducted by ABC Laboratories, Inc. for ADBAC Quat

Joint 

Venture/Chemical Specialties Manufacturers Association.
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 50% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Pimephales promelas

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.36 mg/L Mortality Unknown

Results Remarks: Highly toxic; categorized as highly toxic to fish
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Missing data

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental

Assessment of an Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published 

data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance

Organisms 

Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 50% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Oncorhynchus mykiss

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 1.01 mg/L Mortality Unknown

Results Remarks: Highly/moderately toxic; categorized as highly/moderately toxic to fish

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Data missing

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental

Assessment of an Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published 

data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance

Organisms 

Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 80% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Pimephales promelas

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.28 mg/L Mortality Unknown

Results Remarks: Highly toxic

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 419472-01. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 96-

Hour Toxicity Test 

of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Bluegill Sunfish. Study No.

SC890050. 

Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint

Venture/Chemical 

Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 30% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

EPA OPPTS 850.1075

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Lepomis macrochirus

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

96 Hours

NOEC: = 0.456 mg/L Unknown

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.515 mg/L Mortality Unknown

Results Remarks: Highly toxic

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 419472-01. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 96-
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Hour Toxicity Test 

of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Bluegill Sunfish. Study No.

SC890050. 

Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint

Venture/Chemical 

Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 50% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Menidia beryllina

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Saltwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.31 mg/L Mortality Unknown
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Results Remarks:

Highly toxic; categorized as highly toxic to fish

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Data missing; Non-guideline species

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental

Assessment of an Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published 

data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance

Organisms 

Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 80% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Yes

Species: Pimephales promelas

GLP: No Data
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Guideline deviations include 10 mg/L humic acid added to dilution water

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

96 Hours

NOEC: = 0.53 mg/L Unknown

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.77 mg/L Mortality Unknown

Results Remarks: Highly toxic; categorized as highly toxic to fish

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 437401-02. Sword, M.C. and L. Stuerman. 1993. Static-Renewal Acute Toxicity

of ADBAC to 

Fathead Minnow (Pimephales promelas) in Dilution Water Amended with 10 mg/L Humic

Acid. Study 

No. 41236. Unpublished data. Conducted by ABC Laboratories, Inc. for ADBAC Quat

Joint 

Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC,50% AI

Sponsored

Chemical Result

Read-Across
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Type:

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Daphnia magna

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 48 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Limit Test: Unknown

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % = 0.02 mg/L No Data

Results Remarks: very highly toxic to aquatic invertebrates

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability

Remarks: Data Missing

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental

Assessment of an Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published 

data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance

Organisms 

Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1])
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 80% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Other

Other Species: Eastern oyster 

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Static

Test Vessel: No Data

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 48 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test: Unknown

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % = 0.055 mg/L Mortality No Data

Results Remarks: very highly toxic to aquatic invertebrates

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Reliability

Remarks:

High control mortality

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: MRID 424795-03. Sved, D.W., J.P. Swigert, and G.J. Smith. 1992. A 48-Hour Static

Acute Toxicity Test 

with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in Embryo Larvae of the Eastern

Oyster 

(Crassostrea virginica). Unpublished data. Conducted by Wildlife International Ltd.

for ADBAC Quat 

Joint Venture/Chemical Specialties Manufacturers Assocation.

(EPA-HQ-OPP-2006-0339-0021[1])

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC,80% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Other

Other Species: Mysid shrimp (Mysidopsis bahia)

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Test Vessel: No Data

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test: Unknown

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

96 Hours

NOEC: = 0.047 mg/L Unknown

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value :
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.092 mg/L Mortality No Data

Results Remarks: very highly toxic to aquatic invertebrates

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: MRID 424795-01. Sved, D.W., J.P. Swigert, and G.J. Smith. 1992. A 96-Hour StaticRenewal Acute 

Toxicity Test with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in the Saltwater

Mysid 

(Mysidopsis bahia). Project No. 350A-101A. Unpublished data. Conducted by Wildlife

International 

Ltd. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1])

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 50% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Invertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Yes

Species/In Vitro

System:

Other

Other Species: Mysid shrimp (Mysidopsis bahia)

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Guideline deviations included testing conducted at higher temperature.

Limit Test: Unknown
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.08 mg/L Mortality No Data

Results Remarks: very highly toxic to aquatic invertebrates

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Data missing

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental

Assessment of an Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published 

data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance

Organisms 

Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1])

 Acute Toxicity to Aquatic Invertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 30% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

Method/Guideline

Followed:

EPA OPPTS 850.1010

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Daphnia magna

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 48 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test: Unknown

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours EC 50 % = 0.0059 mg/L No Data

Results Remarks: very highly toxic to aquatic invertebrates

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: MRID 419472 -03. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute

48-Hour Toxicity 

Test of Alkyl Dimethyl Ammonium Chloride (ADBAC) to Daphnia magna. Study No. SC

890052. 

Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint

Venture/Chemical 

Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1])

 Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Identity: Alkyldimethylbenzylammoniumchloride (R = C12-14); abbreviated as BM

Remarks: Purity > 97.5%

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1995

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Chlorella pyrenoidosa

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test

Concentrations:

Unknown

Nominal and

Measured
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Chlorella pyrenoidosa was supplied by the Institute of Molecular and Cellular

Bioscience. The precultures of test alga were incubated at 25 +/- 1 C under

continuous lightening at 140 μE/m2/s and sterile aeration through a 0.2-mm filter

for approximately 7 days. A modified Bristol medium was used.

The concentration ranges in which inhibitory effects were likely to occur were

determined from range-finding tests. The 400-mL culture medium and the algal

inoculums taken from a preculture in the exponential growth phase were added to

500-mL sterile culture bottles. The initial algal density was approximately 1 x

104 cells/mL. The stepwise graded concentrations of BM, with more than four

concentrations with two replicates each , were added to each test solution. The

test solutions were incubated at 24+/- 2 C under continuous lightening at 140

μE/m2/s with slow continuous shaking (100 rpm) for 96 h using controlled

environment incubator shaker. A 100- to 10-mL portion of each test solution was

centrifuged at 1,900 g for 15 min at 5 C. Chlorophyll-a concentration, an

indicator of cell growth, was measured every 24 h from the start of this test to

96 h by Parsons and Strickland's method.

The growth curves for each concentration of test substance and for the control

were obtained by plotting the chlorophyll-a concentration in the ordinate and the

incubation time in the abscissa. Following OECD test guidelines, the areas under

the growth curves were calculated every 24 h until 96 h, and the percentage of

growth inhibition was calculated from the ratio of the difference between the

area under the growth curve at each substance concentration. The 96-h EC50 and

95% confidence level were calculated by Litchfield and Wilcoxon's method.

Limit Test:

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

96 Hours EC 50 % = 0.67 mg/L No

Data

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Utsunomiya, A., T. Watanuki, K. Matsushita, and I. Tomita, "Toxic Effects of

Linear Alkylbenzene Sulfonate, 

Quaternary Alkylammonium Chloride and Their Complexes on Dunaliella sp. and

Chlorella pyrenoidosa". 

Environ.Toxicol.Chem. 16(6):1247-1254; 1997

 Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Identity: ADBAC

Remarks: 50% (2.5 mg/L) plus Bentonite Clay at concentrations of 0:0, 2.5:25,

2.5:37.5, 2.5:50, and 

2.5:75 mg/L
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Selenastrum sp.

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: No Data

Test

Concentrations:

Unknown

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Test Conditions

Remarks:

Limit Test: No

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

96 Hours EC 50 % = 0.67 mg/L Biomass Unknown

Results Remarks: LOAEC = 2.5:25 for growth; NOAEC = 2.5:37.5 for growth

Remarks: 

Supplemental study

Open literature

Assess detoxification of a.i.

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability

Remarks:

deviations from guideline include photoperiod and lack of detailed information

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental

Assessment of an Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published 

data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic

Nuisance Organisms 

Conference, Toronto, Canada.
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

(EPA-HQ-OPP-2006-0339-0021[1])

 Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Identity: Hyamine 3500, synonym for quaternary ammonium compounds, benzyl-C12-

16-

alkyldimethyl, chlorides

Remarks: 80% concentrate in ethanol

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1992

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Other

Other Species: Cladophora glomerata

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test

Concentrations:

Unknown

Nominal and

Measured

Concentrations:

0.2, 0.3, 0.4, 0.5, 0.8 and 1.0 mg/L

Exposure Period: 96 Hours

Vehicle Used:
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Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 20 °C

Photo

(Light/Dark):

12 light: 12 dark

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

The test filamentous species, Cladophora glomerata (L.) Kutz. (UTTC #13) was

obtained from the 

University of Toronto Culture Collection (UTCC). The alga was originally

isolated from the Thames 

River, Ontario. The algal culture was maintained in Bold's Basal Medium (BBM)

(Hutchinson and Stokes 

1975) supplemented with a vitamin mix (B12, biotin and thiamine), and 5 mg/L

Si as NaSiO3 x 9H2O

Chlorella fusca Shihira et Krauss, a unicellular alga, obtained from the

laboratory at the Canada Centre 

for Inland Waters, Burlington, Ontario, was the other species used in the

tests.

Hyamine 3500, was purchased from the Rohm and Haas Company. This algicide is

an 80% concentrate 

in ethanol, containing the active ingredient n-alkyl (80% tetradecyl, 40%

dodecyl and 10% hexadecyl) 

dimethyl benzyl ammonium chloride. The molecular weight of the chemical is

359.6. Stable and 

nonvolatile, it has been recommended by the company (Rohm and Haas 1978) as a

disinfectant in 

swimming pools at low concentrations.

Algal assays were conducted in duplicate in 250-mL Erlenmeyer flasks containing

100 mL BBM 

solution. The toxicity range of Hyamine for Cladophora was tested at the

beginning of the study. In 

the final assays, the Hyamine concentrations were set at 0.2, 0.3, 0.4, 0.5,

0.8 and 1.0 mg/L. Zero 

Hyamine concentration was used as a control. About 0.5 g (wet weight) of

Cladophora filament was 

used as the inoculums. The cultures were incubated for 4 days at 20 C in an

environmental chamber 

where the light and dark cycle was set at 12:12 and the light intensity was 60

μEin/m2/sec. 

On day 4, a small amount of plant material was removed from each concentration

for morphological 

examination using a light microscope. One set of samples was put aside for

electron microscopic 

preparation; the other set was incubated for another 3 weeks for further

morphological evaluations. 

These tests were repeated at the end of the experiment. 
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For electron microscope studies, Cladophora filaments were fixed in 4%

(cacodylate-buffered) 

glutaraldehyde for 6 hr and then postfixed in 2% (cacodylate-buffered) osium

tetroxide for 1 hr. The 

dehydration ethanol series was 30%, 50%, 70%, 90%, 95% and 100%.

For infiltration, the Spurr's medium (Hayat 1989) to ethanol ratios were 1:3,

1:2, and 2:3 v/v. The 

Cladophora filaments were then embedded in 100% Spurr's medium in an aluminum

dish. The samples 

were polymerized at 70 C overnight. 

A dissecting microscope was used to locate the apical cells on the

plastic. After they were marked, 

small columns of specimens were cut out with a jeweller's saw. Thin sections

were prepared with a 

glass knife on a microtome. These sections were then stained with uranyl

acetate and lead citrate. 

Observations were made using a Siemens electron microscope.

To assess the effect of Hyamine on Chlorella cells, a conventional method based

on growth 

measurements was used (Miller et al. 1978). A Chlorella pre-culture was

prepared. The Hyamine 

concentrations used were the same as those employed in the Cladophora

assays. On day 7, when the 

pre-culture reached its logarithmic growth phase, the Chlorella cells were

ready for inoculation. The 

initial cell concentration in the test solution was 40 x103 cells/L. After

inoculation, the culture flasks 

were mounted on a platform shaker and incubated for 14 days in a growth

chamber. Cells numbers 

were enumerated at 4 day intervals and at the end of the experiment when the

algal biomass reached 

its growth plateau.

Limit Test: No

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration
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Results Remarks: test yield after 14 days

conc. (10^3 cells/mL)

control 14.5

0.15 13.6

0.20 15.3

0.30 13.9

0.40 no data

0.50 14.6

0.60 10.8

0.80 no growth

1.00 no growth

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Wong, S.L., and J.F. Wainwright. "Interpretation of Ultrastructural Changes in

Cladophora glomerata 

Resulting from Hyamine Toxicity". Bull.Environ.Contam.Toxicol. 52(3):325-332;

1994

 Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Identity: Alkyldimethylbenzylammoniumchloride (R = C12-14); abbreviated as BM

Remarks: Purity > 97.5%

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1995

Method/Guideline

Followed:

No Data
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Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Dunaliella sp.

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test

Concentrations:

Unknown

Nominal and

Measured

Concentrations:

The test chemical was dissolved with water to make a final concentration between

2 and 0.1 mM.

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Dunaliella sp. isolated from an Antarctic high salt lake by Watanuki et al. was

used to test the biological 

effects of surfactacts by determining glycerol content of the cells. The

precultures of test algae were 

incubtated at 20 +/- 1 C under continuous lightening at 140 μE/m2/s and sterile

aeration through 

0.2-μm filter for approximately 7 days. Modified Johnson's medium was used in

this study. The algal 

culture was taken from a preculture in the exponential growth phase. The initial

cell density was about 

6 x 106 cells/ml, as counted by Microcellcounter F-800. The 200-ml algal

culture and the stepwise 

graded concentration of BM were added to 300-ml culture bottles with two
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replicates of each 

concentration. These cultures were incubated without shaking and aeration of 24

h at 20 +/- 1 C 

under continuous lightening at 140 μE/m2/s using a controlled environmental

incubator shaker. The 

number of cells in the control was almost unchanged from the initial density

during the 24-h 

incubation. The coefficient of the control cells was 15.1%

The glycerol contents at each concentration level of the test chemicals were

measured by H-NMR after 

24 h. The dose-response curves were drawn by plotting the glycerol contents,

which were shown with 

the percent of untreated control without surfactant in the ordinate and the

concentration of surfactant 

in the abscissa. The 24-h EC50 and 95% confidence level were calculated by

Litchfield and Wilcoxon's 

method.

Limit Test:

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

24 Hours EC 50 % = 1.8 mg/L No

Data

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability
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Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Utsunomiya, A., T. Watanuki, K. Matsushita, and I. Tomita, "Toxic Effects of

Linear Alkylbenzene Sulfonate, 

Quaternary Alkylammonium Chloride and Their Complexes on Dunaliella sp. and

Chlorella pyrenoidosa". 

Environ.Toxicol.Chem. 16(6):1247-1254; 1997

 Chronic Aquatic Vertebrate Toxicity

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Chronic Aquatic Vertebrate Toxicity

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 30% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Chronic Aquatic Vertebrate Toxicity

Year Study

Performed:

Method/Guideline

Followed:

EPA OPPTS 850.1400

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Pimephales promelas

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 34 Days
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Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test: Unknown

Test Results - Chronic Aquatic Vertebrate Toxicity

NOEC Exposure

Duration:

34 Days

NOEC: = 0.0322 mg/L Unknown

LOEC Exposure

Duration:

34 Days

LOEC: = 0.0759 mg/L Unknown

NOELR Exposure

Duration:

NOELR: =

LOELR:

LOELR Exposure

Duration:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration
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Results Remarks: LOAEC = 0.0759 mg/L a.i.; NOAEC = 0.0322 mg/L a.i.; MATC = 0.0494 a.i.

Reliability/Data Quality - Chronic Aquatic Vertebrate Toxicity

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Chronic Aquatic Vertebrate Toxicity

Reference: MRID 423021-02. McIntyre, D.O. and H.O. Pate. 1992. Daily Static-Renewal Early

Life Stage Toxicity 

Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Fathead Minnows. Project

No. 

SC890057. Unpublished data. Conducted by Battelle Columbus Operations for ADBAC

Quat Joint 

Venture/Chemical Specialties Manufacturers Assocation.

(EPA-HQ-OPP-2006-0339-0021[1])

 Chronic Aquatic Invertebrate Toxicity

CAS Number : 68424-85-1

Sponsored

Chemical Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

Test Substance - Chronic Aquatic Invertebrate Toxicity

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

ADBAC, 30% AI

Sponsored

Chemical Result

Type:

Read-Across

Test Substance

Result Type:

Unknown

Method - Chronic Aquatic Invertebrate Toxicity

Year Study

Performed:

Method/Guideline

Followed:

EPA OPPTS 850.1300

Other

Method/Guideline:

Deviations from

Method/Guideline:

Yes

Species/In Vitro

System:

Daphnia magna

GLP: No Data
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Analytical

Monitoring:

No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period:

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Numerous deviations from guideline include MATC not established

Limit Test:

Test Results - Chronic Aquatic Invertebrate Toxicity

NOEC Exposure

Duration:

NOEC: = 0.00415 mg/L Unknown

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure
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Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

Results Remarks: LOAEC = Not determined; NOAEC 0.00415 mg/L a.i.; MATC = Not Determined

Reliability/Data Quality - Chronic Aquatic Invertebrate Toxicity

Reliability: 3 - Not Valid

Reliability

Remarks:

High control mortality

Key Study Sponsor

Indicator:

Reference - Chronic Aquatic Invertebrate Toxicity

Reference: MRID 423021-01. McIntyre, D.O. and H.O. Pate. 1992. Daily Static-Renewal Chronic

21-day Toxicity 

Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Daphnia magna. Study

No. SC890056. 

Unpublished data. Conducted by Batalle Columbus Division for ADBAC Quat Joint

Venture/Chemical 

Specialties Manufacturers Assocation.

(EPA-HQ-OPP-2006-0339-0021[1])

 Acute Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name

:

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl,

chlorides

Test Substance - Acute Toxicity

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test Substance

Comments:

Identity: ADBAC

Remarks: Purity 82.26%

Sponsored Chemical Result

Type:

Read-Across

Method - Acute Toxicity
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Route of Administration: Dermal

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

Gender: Both M/F

Number of Animals per

Dose:

10

Dose: 0, 500, 1000 or 2000 mg/kg

Rats (5/sex/dose)

Year Study Performed:

Method/Guideline

Followed:

EPA OPPTS 870.1200

GLP: No Data

Method/Guideline and

Test Condition Remarks:

Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

LD 50 % = 930 mg/kgbw

Number of Deaths ( Male

):

Number of Deaths ( Female

):

Number of Deaths ( Total

):

Results Remarks: LD50 value: 

930 mg/kg (combined)

1100 mg/kg (males)

704 mg/kg (females)

Number of deaths at each dose level: Five 2000 mg/kg females

were found dead on Day 1. One 500 

mg/kg female, one male and four females in the 1000 mg/kg

group, and five 2000 mg/kg were found 

dead by Day 4.

Remarks: Five 2000 mg/kg females were found dead on Day

1. One 500 mg/kg female, one male and 

four females in the 1000 mg/kg group, and five 2000 mg/kg

were found dead by Day 4. Irregular 

respirations, hunched postures, hypoactivity, coldness to the

touch, reduced fecal volume, tremors, 
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ataxia, and/or dyspnea were noted for the decendents prior to

death. Hypoactivity, hunched postures, 

reduced fecal volume, and/or irregular respiration were noted

for some of the surviving rats with 

recovery by Day 2. Erythema and edema were noted at dose

site from most of the rats. Eschar was 

also noted at the dose site from surviving rats. All

surviving rats had normal body weight gains. No 

gross abnormalities were noted from the surviving rats and

one female decedent in the 1000 mg/kg 

group. The other decedents had slightly/moderately red lungs,

discolored livers, and/or 

red/black/green/yellow intestines. 

Conclusion: Toxic Category II

Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity

Reference: 45109202 (MRID) Moore, G. (1999) Acute Dermal Toxicity Study

in Rats: Albemarle BQ451-8 Biocide: Lab 

Project Number: 7466:P322. Unpublished study prepared by

Product Safety Labs. 25p.

(EPA-HQ-OPP-2006-0339-0019[1])

 Acute Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name

:

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl,

chlorides

Test Substance - Acute Toxicity

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test Substance

Comments:

Identity: ADBAC

Remarks: Purity 82.26%

Sponsored Chemical Result

Type:

Read-Across

Method - Acute Toxicity

Route of Administration: Oral

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data
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Gender: Both M/F

Number of Animals per

Dose:

10

Dose: Females: 0, 100, 250, 500 or 510 mg/kg

Males: 0, 500, 510, 525, 600 or 750 mg/kg

Rats (5/sex/dose)

Year Study Performed:

Method/Guideline

Followed:

EPA OPPTS 870.1100

GLP: No Data

Method/Guideline and

Test Condition Remarks:

Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

LD 50 % = 304.5 mg/kgbw

Number of Deaths ( Male

):

Number of Deaths ( Female

):

Number of Deaths ( Total

):

Results Remarks: LD50 value: 

304.5 mg/kg (both sexes combined)

510.9 mg/kg (males)

280.8 mg/kg (females)

Number of deaths at each dose level: Two 200 mg/kg females,

four 500 mg/kg females, three males, 

and four females in the 510 mg/kg group and all males in the

525, 600, and 750 mg/kg groups were 

dead by Day 6.

Remarks: Two 200 mg/kg females, four 500 mg/kg females, three

males, and four females in the 510 

mg/kg group and all males in the 525, 600, and 750 mg/kg

groups were dead by Day 6. Breathing 

abnormalities, decreased activity, wobbly gait, salivation,

apparent hypothermia, soft/mucoid stools, 

diarrhea, decreased/no defecation, fecal/urine stain,

piloerection, rough haircoat, hunched postures, 

dehydration, ocular discharge, decreased food consumption,

and/or dark material around the facial 

area were noted from most of the rats with recovery by Day 13

or earlier. Two surviving 510 mg/kg 

males lost weight during the first week of study, but gained

weight during the second week. All other 
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surviving rats gained weight during the study. The decedents

had abnormal content and/or reddened 

mucosa in the digestive tract, dark red thymus, and/or fluid

contents in the thoracic cavity, abnormal 

content in the small intestines and/or thickened stomach

mucosa. 

Conclusion: Toxic Category II

Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity

Reference: 45109204 (MRID) Bonnette, K. (1998) An Acute Oral Toxicity

Study in Rats with BQ 451-8: Lab Project 

Number: 3192.32. Unpublished study prepared by Springborn

Laboratories. 90 p.

(EPA-HQ-OPP-2006-0339-0019[1]; p. 3)

 Acute Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name

:

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl,

chlorides

Test Substance - Acute Toxicity

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test Substance

Comments:

Identity: ADBAC

Remarks: Purity 82.26%

Sponsored Chemical Result

Type:

Read-Across

Method - Acute Toxicity

Route of Administration: Inhalation

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

Gender: Both M/F

Number of Animals per

Dose:

10

Concentration: 0.054 or 0.51 mg/L
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Rats (5/sex/dose)

Year Study Performed:

Method/Guideline

Followed:

EPA OPPTS 870.1300

GLP: No Data

Method/Guideline and

Test Condition Remarks:

Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

LC 50 % < 0.51 mg/L

Number of Deaths ( Male

):

Number of Deaths ( Female

):

Number of Deaths ( Total

):

Results Remarks: LC50 value: 0.054 < LC50 < 0.51 mg/L

Number of deaths at each dose level: 0.054 mg/L group: All

animals survived; 0.51 mg/L group: All 

animals died. 

Remarks: 0.054 mg/L group: All animals survived. Animal

displayed ocular and nasal discharge, 

irregular respiration, dyspnea, hunched posture, and

hypoactivity. Irregular respiration and/or 

hypoactivity persisted in several rats upon removal from the

exposure chamber and some animals also 

developed facial staining or reduced fecal volume. All rats

recovered from the above symptoms by Day 

2 and appeared active and healthy for the remainder of the

study, gaining bodyweight over the entire 

14-day observation period. No gross abnormalities were noted

in animals when necropsied.

0.51 mg/L group: All animals died. During exposure, animals

exhibited ocular and nasal discharge, 

irregular respiration, dyspnea, gasping, hunched posture, and

hypoactivity prior to death. Gross 

necropsy of one animal revealed discoloration and edema of

the lungs. Gross abnormalities were not 

noted in the other animals.

Conclusion: Toxic Category II

Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions
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Reliability Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity

Reference: 44885201 (MRID) Wnorowski, G. (1999) Acute Inhalation

Toxicity Study in Rats: Albemarle BQ451-8 

Biocide: Lab Project Number: 7467: P330. Unpublished study

prepared by Product Safety Labs. 30 p.

(EPA-HQ-OPP-2006-0339-0019[1])

 Repeated-Dose Toxicity

CAS Number : 68424-85-1

Sponsored Chemical

Name :

Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl,

chlorides

Test Substance - Repeated-Dose Toxicity

Test Substance: No CAS Number Provided

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Identity: ADBAC

Remarks: 79.7% ai 

Sponsored Chemical

Result Type:

Read-Across

Method - Repeated-Dose Toxicity

Route of

Administration:

Oral

Type of Exposure: In Food

Species: Rat

Mammalian Strain: No Data

Gender: Both M/F

Number of Animals

per Dose:

30

Dose: 0, 100, 500, 1000 (0, 6.3, 31.2 and 62.0 mg/kg/day for males and

0, 7.9, 38.3 and 76.7 mg/kg/day for 

females), 4000 or 8000 ppm 

Year Study

Performed:

Method/Guideline

Followed:

EPA OPPTS 870.3100

GLP: No Data

Exposure Period: 90 Days
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Frequency of

Treatment:

daily

Post-Exposure

Period:

Method/Guideline and

Test Condition

Remarks:

In a subchronic oral toxicity study in rats, male and female rats

(15/sex/dose) were administered ADBAC 

(79.7% a.i.) in the diet for 13 weeks at dose levels of 0, 100,

500, 1000, 4000 or 8000 ppm.

Test Results - Repeated-Dose Toxicity

Concentration

(LOAEL/ LOAEC/

NOAEL/ NOAEC) :

LOAEL/

LOAEC/

NOAEL/

NOAEC

Population Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

NOAEL Male = 31.2 mg/kg

bw/day

LOAEL Male = 62 mg/kg

bw/day

NOAEL Female = 76.7 mg/kg

bw/day

LOAEL Female > 76.7 mg/kg

bw/day

Results Remarks: NOAEL (systemic; males) = 500 ppm (31.2 mg/kg/day)

LOAEL (systemic; males) = 1000 ppm (62.0 mg/kg/day) based on

decreased weight gain

NOAEL (systemic; females) = 1000 ppm (76.7 mg/kg/day)

LOAEL (systemic; females) = 4000 ppm (>76.7 mg/kg/day) based on

increased mortality, decreased 

body weight gain and food consumption and increased lesions in

females

Actual dose received by dose level by sex: 0, 6.3, 31.2 and 62.0

mg/kg/day for males and 0, 7.9, 38.3 

and 76.7 mg/kg/day for females for 0, 100, 500 and 1000 ppm,

respectively. Increased mortality at 

the two highest dose levels did not allow for daily compound

consumption to be calculated.

Remarks: Increased mortality was observed at the 4000 and 8000 ppm

dose groups in both sexes. 

Decreased body weight, weight gain, food consumption and increased

incidence of microscopic lesions 

[congestion and edema of the G.I. tract, hemorrhaging of the lungs

and brain] were also observed in 

males and females at 4000 ppm]. 

Conclusion: The systemic NOAEL for males is 500 ppm and for females is 1000

ppm, based upon decreased body 

weight and weight gain in males at 1000 ppm, and increased

mortality, decreased body weight gain and 

food consumption, and increased lesions in females at 4000 ppm.

Reliability/Data Quality - Repeated-Dose Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks: This study is classified as acceptable.

Key Study Sponsor

Indicator:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:29:06 AM]

Reference - Repeated-Dose Toxicity

Reference: 40746601 (MRID) Van Miller, J.; Weaver, E. (1998) Ninety-day

Dietary Toxicity Study with Alkyl 

Dimethyl Benzyl Ammonium Chloride (ADBAC) in Rats: Project ID:51-

503. Unpublished study prepared 

by Bushy Run Re-search Center. 300p.

(EPA-HQ-OPP-2006-0339-0019[1])