7/8/2014 ARS Project: GENOME SEQUENCING AND IDENTIFICATION OF VIRULENCE FACTORS IN FLAVOBACTERIUM PSYCHROPHILUM (408300) A...

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Research Project: GENOME SEQUENCING AND
IDENTIFICATION OF VIRULENCE FACTORS IN

FLAVOBACTERIUM PSYCHROPHILUM

Location: Leetown, West Virginia
2008 Annual Report

1a.Objectives (from AD-416)

The objective of this cooperative research project is to identify
virulence factors in the genome of Flavobacterium psychrophilum by
use of DNA sequencing. Flavobacterium psychrophilum is the
etiological agent of bacterial cold water disease. The disease causes
significant production losses of rainbow trout and novel methods are
urgently needed for control of this disease. Knowedge of the F.
psychrophilum genome should serve as a foundation for future
development of new vaccines, improved diagnostic tests, and novel
therapeutics.

1b.Approach (from AD-416)

Genomic DNA will be isolated from a virulent clone of Flavobacterium
psychrophilum strain CSF-259-93 and random, large-insert and small-
insert DNA libraries will be created. The DNA libraries will be used to
generate clones for 10,000 sequencing reactions for low-coverage
sequence analysis of the genome. Sequence data will be organized
into contigs and preliminary annotation will be performed using the
ERGO bioinformatics system developed by Integrated Genomics.
Preliminary annotation will allow assessment of the metabolic and
genetic organization of Flavobacterium psychrophilum. The partially
assembled genome sequence will be available on the NCCCWA web
site and a reference clone bank established.

3.Progress Report

Bacterial cold water disease, caused by Flavobacterium psychrophilum,
is a serious problem for rainbow trout aquaculture. Conventional
approaches to vaccine development have been ineffective and
antimicrobial resistance is of concer. The determination of the
complete genome sequence last year was the first step toward a
better understanding of metabolic and pathogenic processes, and the
identification of vaccine candidates. This year, we reported the
identification of 96 potential virulence factors from the genome
sequence of Flavobacterium psychrophilum and overexpression of
several as recombinant proteins. The ADODR is in frequent contact
with the cooperator by phone and e-mail. This work addresses
research outlined in the National Program 106, Component 4,
Integrated Aquatic Animal Health Managment.

Last Modified: 05/14/2009

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Project Team
Wiens, Gregory - Greg

Scott LaPatra - Principal
° Investigator

Project Annual Reports
o FY 2008
o FY 2007
o FY 2006
o FY 2005
o FY 2004

Related National Programs
© Aquaculture (106)

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