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**1a.Objectives (from AD-416)**													The objective of this cooperative research project is to conduct geo-spatial analyses of climatic and bio-geographic factors that influence the distribution and vector competence of Culex tarsalis in the inter-mountain West. This mosquito species  is a very competent vector of WNV, and using GIS to analyze and interpret climatic and bio-geographic factors that support mosquito populations will define spatial patterns where risk for exposure to WNV is high for humans, livestock, and wildlife populations.  The same methods will be applied to assess risk of a potential Rift Valley fever virus introduction.						
**1b.Approach  (from AD-416)**													The goal is to be able to predict areas and conditions of high and low risk for WNV.  This will focus mosquito control programs and call attention to the need for use of personal protection methods (insect repellents). Vector-borne diseases are the product of habitats that support vector populations, ambient temperatures that enhance rates of larval development and virus amplification, and wildlife species that serve as donors of virus ingested during vector blood feeding. Using GIS, it will be possible to predict geographic areas and seasonal patterns that are necessary for outbreaks  of arbovirus to occur. 
GIS methods provide powerful tools for spatial analysis of  climatic and biogeographic factors that promote vector competence among mosquito populations.  We will use satellite imagery and ground-based data to define environmental profiles for habitats and areas that support populations of C. tarsalis.  These data will define most suitable habitats and best conditions for the species and serve as a basis for predictions of where and when this mosquito is most abundant on a larger spatial scale.  Climatic data superposed on the spatial abundance of C. tarsalis will enable predictions of maximum risk for WNV transmission, and potentially, Rift Valley fever virus.						
**3.Progress Report**													This report documents research conducted under a Specific Cooperative Agreement between ARS and the UNIV OF WYOMING.  Additional details for the research can be found in the report for the parent project 5410-32000-014-00D, VECTOR COMPETENCE AND PROTECTION OF U.S. LIVESTOCK AND WILDLIFE FROM ARTHROPOD-BORNE DISEASES							  										The degree day modeling project was field tested in California in 2007-2008. The model was modified to include new data on virus replication rates and host susceptibility.  These changes were incorporated into a new model.  The model was further modified to match some field conditions and a higher resolution thermal profile was acquired.  This new model had an excellent match to field data in CA.  Data for additional states are being added to the model to further validate its use.  The computer model was submitted to the ARS for public dissemination and is in the clearance process.  This research supports NP104 Action Plan Component 1: Ecology and Epidemiology.  ADODR monitoring activities to evaluate research progress were through meetings, email and phone calls.						
    
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