### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] You are here: EPA Home Prevention, Pesticides & Toxic Substances Pollution Prevention & Toxics High Production Volume (HPV) Challenge High Production Volume Information System (HPVIS) Submission Report 

# High Production Volume Information System (HPVIS)

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# Submission Report

Submission Information

Submission ID : 25349470 Submission Name : Quaternary ammonium compounds, benzyl-C12-16- alkyldimethyl, chlorides

AR Number :



## Chemical Information

CAS Number : 68424-85-1 Ninth Collective Index Name : Quaternary ammonium compounds, benzyl-C12- 16-alkyldimethyl, chlorides



## Submitter Information

Submitter's Name : EPA

Address : City : Washington

State : DC
ZIP/Postal Code : Country : Type of Submitter : Company 

## Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address : 

## Additional Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address :



## Sponsor Information
Sponsor's Name : EPA

Address : City :
State : ZIP/Postal Code : Country :
Phone Number :

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Fax Number :
E-Mail Address : Type of Sponsor : Company 

## Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address :



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 80% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 850.1075

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Cyprinodon variegatus

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Saltwater

Test Concentrations: No Data

Nominal and Measured Concentrations:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.86 mg/L Mortality Unknown

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Results Remarks: LC50 = 0.86 mg/L (a.i.); NOAEC = 0.68 mg/L (a.i.) Highly toxic; categorized as highly toxic to fish 

### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 424795-02. Sved, D.W., J.P. Swigert, and G.J. Smith. 1992. A 96-Hour Static
Renewal Acute Toxicity Test with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in the Sheepshead Minno (Cyprinodon variegatus). Project No. 350A-102. Unpublished data. Conducted by Wildlife International Ltd. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 30% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Species: Oncorhynchus mykiss

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration: 96 Hours

NOEC: = 0.619 mg/L Unknown

LOEC Exposure Duration:

LOEC:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.923 mg/L Mortality Unknown

Results Remarks: Highly toxic; categorized as highly toxic to fish



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 419472-02. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 96- Hour Toxicity Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Rainbow Trout. Study No.
SC890051. Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition ADBAC, 80% AI

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] and Other Test Substance Comments:
Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: Yes

Species: Pimephales promelas

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Water Hardness:

Method/Guideline Test Conditions Remarks: Deviations from guideline include additions of 20 mg/L humic acid to dilution water

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration: 96 Hours

NOEC: = 0.99 mg/L Unknown

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 1.4 mg/L Mortality Unknown

Results Remarks: Moderately toxic; categorized as moderately toxic to fish



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 437401-01. Sword, M.C. and L. Stuerman. 1993. Static-Renewal Acute Toxicity
of ADBAC to Fathead Minnow (Pimephales promelas) in Dilution Water Amended with 20 mg/L Humic Acid. Study
No. 41235. Unpublished data. Conducted by ABC Laboratories, Inc. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] (EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 50% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Pimephales promelas

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.36 mg/L Mortality Unknown

Results Remarks: Highly toxic; categorized as highly toxic to fish

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Missing data

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental Assessment of an Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published
data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance Organisms Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 50% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Oncorhynchus mykiss

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 1.01 mg/L Mortality Unknown

Results Remarks: Highly/moderately toxic; categorized as highly/moderately toxic to fish



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Data missing

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental Assessment of an Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published
data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance Organisms Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 80% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Pimephales promelas

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.28 mg/L Mortality Unknown

Results Remarks: Highly toxic



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 419472-01. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 96- Hour Toxicity Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Bluegill Sunfish. Study No.
SC890050. Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 30% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 850.1075

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Lepomis macrochirus

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration: 96 Hours

NOEC: = 0.456 mg/L Unknown

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.515 mg/L Mortality Unknown

Results Remarks: Highly toxic



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 419472-01. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 96-

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Hour Toxicity Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Bluegill Sunfish. Study No.
SC890050. Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 50% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Menidia beryllina

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Saltwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.31 mg/L Mortality Unknown

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Results Remarks: Highly toxic; categorized as highly toxic to fish 

### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Data missing; Non-guideline species

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental Assessment of an Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published
data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance Organisms Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 80% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: Yes

Species: Pimephales promelas

GLP: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Guideline deviations include 10 mg/L humic acid added to dilution water

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration: 96 Hours

NOEC: = 0.53 mg/L Unknown

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.77 mg/L Mortality Unknown

Results Remarks: Highly toxic; categorized as highly toxic to fish



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: MRID 437401-02. Sword, M.C. and L. Stuerman. 1993. Static-Renewal Acute Toxicity
of ADBAC to Fathead Minnow (Pimephales promelas) in Dilution Water Amended with 10 mg/L Humic Acid. Study
No. 41236. Unpublished data. Conducted by ABC Laboratories, Inc. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1].pdf)



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC,50% AI

Sponsored Chemical Result Read-Across

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Type:

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Daphnia magna

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 48 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Limit Test: Unknown



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % = 0.02 mg/L No Data

Results Remarks: very highly toxic to aquatic invertebrates



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Data Missing
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental Assessment of an Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published
data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance Organisms Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1])

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] 

## Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 80% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Other

Other Species: Eastern oyster

GLP: No Data

Analytical Monitoring: No Data

Test Type: Static

Test Vessel: No Data

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 48 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test: Unknown



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % = 0.055 mg/L Mortality No Data

Results Remarks: very highly toxic to aquatic invertebrates



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Reliability Remarks: High control mortality

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: MRID 424795-03. Sved, D.W., J.P. Swigert, and G.J. Smith. 1992. A 48-Hour Static Acute Toxicity Test with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in Embryo Larvae of the Eastern Oyster (Crassostrea virginica). Unpublished data. Conducted by Wildlife International Ltd. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Assocation.

(EPA-HQ-OPP-2006-0339-0021[1])



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC,80% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Other

Other Species: Mysid shrimp (Mysidopsis bahia)

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Test Vessel: No Data

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test: Unknown



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration: 96 Hours

NOEC: = 0.047 mg/L Unknown

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value :

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.092 mg/L Mortality No Data

Results Remarks: very highly toxic to aquatic invertebrates



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: MRID 424795-01. Sved, D.W., J.P. Swigert, and G.J. Smith. 1992. A 96-Hour StaticRenewal Acute Toxicity Test with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in the Saltwater Mysid (Mysidopsis bahia). Project No. 350A-101A. Unpublished data. Conducted by Wildlife International Ltd. for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1])



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 50% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Invertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: Yes

Species/In Vitro System: Other

Other Species: Mysid shrimp (Mysidopsis bahia)

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Guideline deviations included testing conducted at higher temperature.

Limit Test: Unknown

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.08 mg/L Mortality No Data

Results Remarks: very highly toxic to aquatic invertebrates



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Data missing

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental Assessment of an Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published
data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance Organisms Conference, Toronto, Canada.

(EPA-HQ-OPP-2006-0339-0021[1])



## Acute Toxicity to Aquatic Invertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 30% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 850.1010

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Daphnia magna

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 48 Hours

Vehicle Used: No Data

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test: Unknown



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours EC 50 % = 0.0059 mg/L No Data

Results Remarks: very highly toxic to aquatic invertebrates



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: MRID 419472 -03. Pate, H.O. and D.O. McIntyre. 1991. Daily Static-Renewal Acute 48-Hour Toxicity Test of Alkyl Dimethyl Ammonium Chloride (ADBAC) to Daphnia magna. Study No. SC
890052. Unpublished data. Conducted by Batelle Columbus Division for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Association.

(EPA-HQ-OPP-2006-0339-0021[1])



## Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: Alkyldimethylbenzylammoniumchloride (R = C12-14); abbreviated as BM Remarks: Purity > 97.5%

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1995

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Chlorella pyrenoidosa

GLP: No Data

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test Concentrations: Unknown

Nominal and Measured

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Chlorella pyrenoidosa was supplied by the Institute of Molecular and Cellular Bioscience. The precultures of test alga were incubated at 25 +/- 1 C under continuous lightening at 140 μE/m2/s and sterile aeration through a 0.2-mm filter for approximately 7 days. A modified Bristol medium was used.

The concentration ranges in which inhibitory effects were likely to occur were determined from range-finding tests. The 400-mL culture medium and the algal inoculums taken from a preculture in the exponential growth phase were added to 500-mL sterile culture bottles. The initial algal density was approximately 1 x 104 cells/mL. The stepwise graded concentrations of BM, with more than four concentrations with two replicates each , were added to each test solution. The test solutions were incubated at 24+/- 2 C under continuous lightening at 140 μE/m2/s with slow continuous shaking (100 rpm) for 96 h using controlled environment incubator shaker. A 100- to 10-mL portion of each test solution was centrifuged at 1,900 g for 15 min at 5 C. Chlorophyll-a concentration, an indicator of cell growth, was measured every 24 h from the start of this test to 96 h by Parsons and Strickland's method.

The growth curves for each concentration of test substance and for the control were obtained by plotting the chlorophyll-a concentration in the ordinate and the incubation time in the abscissa. Following OECD test guidelines, the areas under the growth curves were calculated every 24 h until 96 h, and the percentage of growth inhibition was calculated from the ratio of the difference between the area under the growth curve at each substance concentration. The 96-h EC50 and 95% confidence level were calculated by Litchfield and Wilcoxon's method.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

96 Hours EC 50 % = 0.67 mg/L No Data

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Utsunomiya, A., T. Watanuki, K. Matsushita, and I. Tomita, "Toxic Effects of Linear Alkylbenzene Sulfonate, Quaternary Alkylammonium Chloride and Their Complexes on Dunaliella sp. and Chlorella pyrenoidosa". Environ.Toxicol.Chem. 16(6):1247-1254; 1997



## Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: ADBAC

Remarks: 50% (2.5 mg/L) plus Bentonite Clay at concentrations of 0:0, 2.5:25, 2.5:37.5, 2.5:50, and 2.5:75 mg/L

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Selenastrum sp.

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: No Data

Test Concentrations: Unknown

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Test Conditions Remarks:

Limit Test: No



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

96 Hours EC 50 % = 0.67 mg/L Biomass Unknown

Results Remarks: LOAEC = 2.5:25 for growth; NOAEC = 2.5:37.5 for growth
Remarks: Supplemental study Open literature Assess detoxification of a.i.



### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability Remarks: deviations from guideline include photoperiod and lack of detailed information

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Dobbs, M.G., D.S. Cherry, J.C. Scott, and J.C. Petrille. 1995. Environmental Assessment of an Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) Based Molluscicide Using Laboratory

Tests. Published
data. Proceedings of the Fifth International Zebra Mussel and Other Aquatic Nuisance Organisms Conference, Toronto, Canada.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] (EPA-HQ-OPP-2006-0339-0021[1])



## Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: Hyamine 3500, synonym for quaternary ammonium compounds, benzyl-C12-
16- alkyldimethyl, chlorides Remarks: 80% concentrate in ethanol

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1992

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Other

Other Species: Cladophora glomerata

GLP: No Data

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test Concentrations: Unknown

Nominal and Measured Concentrations: 0.2, 0.3, 0.4, 0.5, 0.8 and 1.0 mg/L

Exposure Period: 96 Hours

Vehicle Used:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 20 °C

Photo (Light/Dark): 12 light: 12 dark

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: The test filamentous species, Cladophora glomerata (L.) Kutz. (UTTC #13) was obtained from the University of Toronto Culture Collection (UTCC). The alga was originally isolated from the Thames River, Ontario. The algal culture was maintained in Bold's Basal Medium (BBM) (Hutchinson and Stokes 1975) supplemented with a vitamin mix (B12, biotin and thiamine), and 5 mg/L Si as NaSiO3 x 9H2O

Chlorella fusca Shihira et Krauss, a unicellular alga, obtained from the laboratory at the Canada Centre for Inland Waters, Burlington, Ontario, was the other species used in the

tests.

Hyamine 3500, was purchased from the Rohm and Haas Company. This algicide is
an 80% concentrate in ethanol, containing the active ingredient n-alkyl (80% tetradecyl, 40% dodecyl and 10% hexadecyl) dimethyl benzyl ammonium chloride. The molecular weight of the chemical is
359.6. Stable and nonvolatile, it has been recommended by the company (Rohm and Haas 1978) as a disinfectant in swimming pools at low concentrations.

Algal assays were conducted in duplicate in 250-mL Erlenmeyer flasks containing
100 mL BBM solution. The toxicity range of Hyamine for Cladophora was tested at the beginning of the study. In the final assays, the Hyamine concentrations were set at 0.2, 0.3, 0.4, 0.5, 0.8 and 1.0 mg/L. Zero Hyamine concentration was used as a control. About 0.5 g (wet weight) of Cladophora filament was used as the inoculums. The cultures were incubated for 4 days at 20 C in an environmental chamber where the light and dark cycle was set at 12:12 and the light intensity was 60 μEin/m2/sec.

On day 4, a small amount of plant material was removed from each concentration for morphological examination using a light microscope. One set of samples was put aside for electron microscopic preparation; the other set was incubated for another 3 weeks for further morphological evaluations. These tests were repeated at the end of the experiment.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] For electron microscope studies, Cladophora filaments were fixed in 4% (cacodylate-buffered) glutaraldehyde for 6 hr and then postfixed in 2% (cacodylate-buffered) osium tetroxide for 1 hr. The dehydration ethanol series was 30%, 50%, 70%, 90%, 95% and 100%.

For infiltration, the Spurr's medium (Hayat 1989) to ethanol ratios were 1:3, 1:2, and 2:3 v/v. The Cladophora filaments were then embedded in 100% Spurr's medium in an aluminum dish. The samples were polymerized at 70 C overnight.

A dissecting microscope was used to locate the apical cells on the plastic. After they were marked, small columns of specimens were cut out with a jeweller's saw. Thin sections were prepared with a glass knife on a microtome. These sections were then stained with uranyl acetate and lead citrate. Observations were made using a Siemens electron microscope.

To assess the effect of Hyamine on Chlorella cells, a conventional method based on growth measurements was used (Miller et al. 1978). A Chlorella pre-culture was prepared. The Hyamine concentrations used were the same as those employed in the Cladophora

assays. On day 7, when the pre-culture reached its logarithmic growth phase, the Chlorella cells were ready for inoculation. The initial cell concentration in the test solution was 40 x103 cells/L. After inoculation, the culture flasks were mounted on a platform shaker and incubated for 14 days in a growth
chamber. Cells numbers were enumerated at 4 day intervals and at the end of the experiment when the algal biomass reached its growth plateau.

Limit Test: No



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Results Remarks: test yield after 14 days

conc. (10^3 cells/mL) control 14.5
0.15 13.6
0.20 15.3
0.30 13.9 0.40 no data
0.50 14.6
0.60 10.8 0.80 no growth 1.00 no growth



### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Wong, S.L., and J.F. Wainwright. "Interpretation of Ultrastructural Changes in Cladophora glomerata Resulting from Hyamine Toxicity". Bull.Environ.Contam.Toxicol. 52(3):325-332;
1994



## Acute Toxicity to Aquatic Plants

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: Alkyldimethylbenzylammoniumchloride (R = C12-14); abbreviated as BM Remarks: Purity > 97.5%

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1995

Method/Guideline Followed: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Dunaliella sp.

GLP: No Data

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test Concentrations: Unknown

Nominal and Measured Concentrations: The test chemical was dissolved with water to make a final concentration between
2 and 0.1 mM.

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Dunaliella sp. isolated from an Antarctic high salt lake by Watanuki et al. was used to test the biological effects of surfactacts by determining glycerol content of the cells. The precultures of test algae were incubtated at 20 +/- 1 C under continuous lightening at 140 μE/m2/s and sterile aeration through 0.2-μm filter for approximately 7 days. Modified Johnson's medium was used in this study. The algal culture was taken from a preculture in the exponential growth phase. The initial cell density was about 6 x 106 cells/ml, as counted by Microcellcounter F-800. The 200-ml algal culture and the stepwise graded concentration of BM were added to 300-ml culture bottles with two

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] replicates of each concentration. These cultures were incubated without shaking and aeration of 24 h at 20 +/- 1 C under continuous lightening at 140 μE/m2/s using a controlled environmental incubator shaker. The number of cells in the control was almost unchanged from the initial density during the 24-h incubation. The coefficient of the control cells was 15.1%

The glycerol contents at each concentration level of the test chemicals were measured by H-NMR after
24 h. The dose-response curves were drawn by plotting the glycerol contents, which were shown with the percent of untreated control without surfactant in the ordinate and the concentration of surfactant in the abscissa. The 24-h EC50 and 95% confidence level were calculated by Litchfield and Wilcoxon's
method.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

24 Hours EC 50 % = 1.8 mg/L No Data

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Remarks:
Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Utsunomiya, A., T. Watanuki, K. Matsushita, and I. Tomita, "Toxic Effects of Linear Alkylbenzene Sulfonate, Quaternary Alkylammonium Chloride and Their Complexes on Dunaliella sp. and Chlorella pyrenoidosa". Environ.Toxicol.Chem. 16(6):1247-1254; 1997



## Chronic Aquatic Vertebrate Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Chronic Aquatic Vertebrate Toxicity

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 30% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Chronic Aquatic Vertebrate Toxicity

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 850.1400

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Pimephales promelas

GLP: No Data

Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 34 Days

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test: Unknown



### Test Results - Chronic Aquatic Vertebrate Toxicity

NOEC Exposure Duration: 34 Days

NOEC: = 0.0322 mg/L Unknown

LOEC Exposure Duration: 34 Days

LOEC: = 0.0759 mg/L Unknown

NOELR Exposure Duration:

NOELR: =

LOELR:

LOELR Exposure Duration:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Results Remarks: LOAEC = 0.0759 mg/L a.i.; NOAEC = 0.0322 mg/L a.i.; MATC = 0.0494 a.i.



### Reliability/Data Quality - Chronic Aquatic Vertebrate Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks:
Key Study Sponsor Indicator: 

### Reference - Chronic Aquatic Vertebrate Toxicity

Reference: MRID 423021-02. McIntyre, D.O. and H.O. Pate. 1992. Daily Static-Renewal Early Life Stage Toxicity Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Fathead Minnows. Project

No. SC890057. Unpublished data. Conducted by Battelle Columbus Operations for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Assocation.

(EPA-HQ-OPP-2006-0339-0021[1])



## Chronic Aquatic Invertebrate Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides



### Test Substance - Chronic Aquatic Invertebrate Toxicity

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: ADBAC, 30% AI

Sponsored Chemical Result Type: Read-Across

Test Substance Result Type: Unknown



### Method - Chronic Aquatic Invertebrate Toxicity

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 850.1300

Other Method/Guideline:

Deviations from Method/Guideline: Yes

Species/In Vitro System: Daphnia magna

GLP: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Analytical Monitoring: No Data

Test Type: Semi-Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period:

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):
Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Numerous deviations from guideline include MATC not established

Limit Test:



### Test Results - Chronic Aquatic Invertebrate Toxicity

NOEC Exposure Duration:

NOEC: = 0.00415 mg/L Unknown

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

Results Remarks: LOAEC = Not determined; NOAEC 0.00415 mg/L a.i.; MATC = Not Determined



### Reliability/Data Quality - Chronic Aquatic Invertebrate Toxicity

Reliability: 3 - Not Valid

Reliability Remarks: High control mortality

Key Study Sponsor Indicator: 

### Reference - Chronic Aquatic Invertebrate Toxicity

Reference: MRID 423021-01. McIntyre, D.O. and H.O. Pate. 1992. Daily Static-Renewal Chronic 21-day Toxicity Test of Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to Daphnia magna. Study
No. SC890056. Unpublished data. Conducted by Batalle Columbus Division for ADBAC Quat Joint Venture/Chemical Specialties Manufacturers Assocation.

(EPA-HQ-OPP-2006-0339-0021[1])



## Acute Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name

: Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides


### Test Substance - Acute Toxicity

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: ADBAC

Remarks: Purity 82.26%

Sponsored Chemical Result Type: Read-Across



### Method - Acute Toxicity

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Route of Administration: Dermal

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

Gender: Both M/F

Number of Animals per Dose: 10

Dose: 0, 500, 1000 or 2000 mg/kg Rats (5/sex/dose)

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 870.1200

GLP: No Data

Method/Guideline and Test Condition Remarks: 

### Test Results - Acute Toxicity
Concentration (LC/LD) : LC/LD % Value Description Value/Lower Concentration Upper Concentration Units

LD 50 % = 930 mg/kgbw

Number of Deaths ( Male ):
Number of Deaths ( Female ):
Number of Deaths ( Total ):

Results Remarks: LD50 value: 930 mg/kg (combined) 1100 mg/kg (males) 704 mg/kg (females)

Number of deaths at each dose level: Five 2000 mg/kg females were found dead on Day 1. One 500 mg/kg female, one male and four females in the 1000 mg/kg group, and five 2000 mg/kg were found dead by Day 4.

Remarks: Five 2000 mg/kg females were found dead on Day
1. One 500 mg/kg female, one male and four females in the 1000 mg/kg group, and five 2000 mg/kg were found dead by Day 4. Irregular respirations, hunched postures, hypoactivity, coldness to the touch, reduced fecal volume, tremors,

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] ataxia, and/or dyspnea were noted for the decendents prior to
death. Hypoactivity, hunched postures, reduced fecal volume, and/or irregular respiration were noted for some of the surviving rats with recovery by Day 2. Erythema and edema were noted at dose site from most of the rats. Eschar was also noted at the dose site from surviving rats. All surviving rats had normal body weight gains. No gross abnormalities were noted from the surviving rats and one female decedent in the 1000 mg/kg

group. The other decedents had slightly/moderately red lungs, discolored livers, and/or red/black/green/yellow intestines.

Conclusion: Toxic Category II



### Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity

Reference: 45109202 (MRID) Moore, G. (1999) Acute Dermal Toxicity Study in Rats: Albemarle BQ451-8 Biocide: Lab Project Number: 7466:P322. Unpublished study prepared by Product Safety Labs. 25p.

(EPA-HQ-OPP-2006-0339-0019[1])



## Acute Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name

: Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides


### Test Substance - Acute Toxicity

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: ADBAC

Remarks: Purity 82.26%

Sponsored Chemical Result Type: Read-Across



### Method - Acute Toxicity

Route of Administration: Oral

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Gender: Both M/F

Number of Animals per Dose: 10

Dose: Females: 0, 100, 250, 500 or 510 mg/kg Males: 0, 500, 510, 525, 600 or 750 mg/kg Rats (5/sex/dose)

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 870.1100

GLP: No Data

Method/Guideline and Test Condition Remarks: 

### Test Results - Acute Toxicity
Concentration (LC/LD) : LC/LD % Value Description Value/Lower Concentration Upper Concentration Units

LD 50 % = 304.5 mg/kgbw

Number of Deaths ( Male ):
Number of Deaths ( Female ):
Number of Deaths ( Total ):

Results Remarks: LD50 value: 304.5 mg/kg (both sexes combined) 510.9 mg/kg (males) 280.8 mg/kg (females)

Number of deaths at each dose level: Two 200 mg/kg females, four 500 mg/kg females, three males, and four females in the 510 mg/kg group and all males in the 525, 600, and 750 mg/kg groups were dead by Day 6.

Remarks: Two 200 mg/kg females, four 500 mg/kg females, three males, and four females in the 510 mg/kg group and all males in the 525, 600, and 750 mg/kg groups were dead by Day 6. Breathing abnormalities, decreased activity, wobbly gait, salivation, apparent hypothermia, soft/mucoid stools, diarrhea, decreased/no defecation, fecal/urine stain, piloerection, rough haircoat, hunched postures, dehydration, ocular discharge, decreased food consumption, and/or dark material around the facial area were noted from most of the rats with recovery by Day 13 or earlier. Two surviving 510 mg/kg males lost weight during the first week of study, but gained weight during the second week. All other

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] surviving rats gained weight during the study. The decedents had abnormal content and/or reddened mucosa in the digestive tract, dark red thymus, and/or fluid contents in the thoracic cavity, abnormal content in the small intestines and/or thickened stomach

mucosa.

Conclusion: Toxic Category II



### Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity

Reference: 45109204 (MRID) Bonnette, K. (1998) An Acute Oral Toxicity Study in Rats with BQ 451-8: Lab Project
Number: 3192.32. Unpublished study prepared by Springborn Laboratories. 90 p.

(EPA-HQ-OPP-2006-0339-0019[1]; p. 3)



## Acute Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name

: Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides


### Test Substance - Acute Toxicity

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: ADBAC

Remarks: Purity 82.26%

Sponsored Chemical Result Type: Read-Across



### Method - Acute Toxicity

Route of Administration: Inhalation

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

Gender: Both M/F

Number of Animals per Dose: 10

Concentration: 0.054 or 0.51 mg/L

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Rats (5/sex/dose)

Year Study Performed:

Method/Guideline Followed: EPA OPPTS 870.1300

GLP: No Data

Method/Guideline and Test Condition Remarks: 

### Test Results - Acute Toxicity
Concentration (LC/LD) : LC/LD % Value Description Value/Lower Concentration Upper Concentration Units

LC 50 % < 0.51 mg/L

Number of Deaths ( Male ):
Number of Deaths ( Female ):
Number of Deaths ( Total ):

Results Remarks: LC50 value: 0.054 < LC50 < 0.51 mg/L

Number of deaths at each dose level: 0.054 mg/L group: All animals survived; 0.51 mg/L group: All animals died.

Remarks: 0.054 mg/L group: All animals survived. Animal displayed ocular and nasal discharge, irregular respiration, dyspnea, hunched posture, and hypoactivity. Irregular respiration and/or hypoactivity persisted in several rats upon removal from the exposure chamber and some animals also developed facial staining or reduced fecal volume. All rats recovered from the above symptoms by Day 2 and appeared active and healthy for the remainder of the study, gaining bodyweight over the entire 14-day observation period. No gross abnormalities were noted in animals when necropsied.

0.51 mg/L group: All animals died. During exposure, animals exhibited ocular and nasal discharge, irregular respiration, dyspnea, gasping, hunched posture, and hypoactivity prior to death. Gross necropsy of one animal revealed discoloration and edema of the lungs. Gross abnormalities were not noted in the other animals.

Conclusion: Toxic Category II



### Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Reliability Remarks:

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity

Reference: 44885201 (MRID) Wnorowski, G. (1999) Acute Inhalation Toxicity Study in Rats: Albemarle BQ451-8 Biocide: Lab Project Number: 7467: P330. Unpublished study prepared by Product Safety Labs. 30 p.

(EPA-HQ-OPP-2006-0339-0019[1])



## Repeated-Dose Toxicity

CAS Number : 68424-85-1

Sponsored Chemical Name : Quaternary ammonium compounds, benzyl-C12-16-alkyldimethyl, chlorides


### Test Substance - Repeated-Dose Toxicity

Test Substance: No CAS Number Provided

Test Substance Purity/Composition and Other Test Substance Comments: Identity: ADBAC Remarks: 79.7% ai

Sponsored Chemical Result Type: Read-Across



### Method - Repeated-Dose Toxicity

Route of Administration: Oral

Type of Exposure: In Food

Species: Rat

Mammalian Strain: No Data

Gender: Both M/F

Number of Animals per Dose: 30

Dose: 0, 100, 500, 1000 (0, 6.3, 31.2 and 62.0 mg/kg/day for males and 0, 7.9, 38.3 and 76.7 mg/kg/day for females), 4000 or 8000 ppm
Year Study Performed:

Method/Guideline Followed: EPA OPPTS 870.3100

GLP: No Data

Exposure Period: 90 Days

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Frequency of Treatment: daily

Post-Exposure Period:

Method/Guideline and Test Condition Remarks: In a subchronic oral toxicity study in rats, male and female rats (15/sex/dose) were administered ADBAC (79.7% a.i.) in the diet for 13 weeks at dose levels of 0, 100, 500, 1000, 4000 or 8000 ppm.



### Test Results - Repeated-Dose Toxicity

Concentration (LOAEL/ LOAEC/ NOAEL/ NOAEC) : LOAEL/ LOAEC/ NOAEL/ NOAEC Population Value Description Value/Lower Concentration Upper Concentration Units

NOAEL Male = 31.2 mg/kg bw/day
LOAEL Male = 62 mg/kg bw/day
NOAEL Female = 76.7 mg/kg bw/day
LOAEL Female > 76.7 mg/kg bw/day

Results Remarks: NOAEL (systemic; males) = 500 ppm (31.2 mg/kg/day) LOAEL (systemic; males) = 1000 ppm (62.0 mg/kg/day) based on decreased weight gain NOAEL (systemic; females) = 1000 ppm (76.7 mg/kg/day) LOAEL (systemic; females) = 4000 ppm (>76.7 mg/kg/day) based on increased mortality, decreased body weight gain and food consumption and increased lesions in
females

Actual dose received by dose level by sex: 0, 6.3, 31.2 and 62.0 mg/kg/day for males and 0, 7.9, 38.3 and 76.7 mg/kg/day for females for 0, 100, 500 and 1000 ppm, respectively. Increased mortality at the two highest dose levels did not allow for daily compound consumption to be calculated.

Remarks: Increased mortality was observed at the 4000 and 8000 ppm dose groups in both sexes. Decreased body weight, weight gain, food consumption and increased incidence of microscopic lesions [congestion and edema of the G.I. tract, hemorrhaging of the lungs and brain] were also observed in males and females at 4000 ppm].

Conclusion: The systemic NOAEL for males is 500 ppm and for females is 1000 ppm, based upon decreased body weight and weight gain in males at 1000 ppm, and increased mortality, decreased body weight gain and food consumption, and increased lesions in females at 4000 ppm. 

### Reliability/Data Quality - Repeated-Dose Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks: This study is classified as acceptable.

Key Study Sponsor Indicator:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:29:06 AM] Reference - Repeated-Dose Toxicity

Reference: 40746601 (MRID) Van Miller, J.; Weaver, E. (1998) Ninety-day Dietary Toxicity Study with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) in Rats: Project ID:51- 503. Unpublished study prepared by Bushy Run Re-search Center. 300p.

(EPA-HQ-OPP-2006-0339-0019[1])