NISTIR 7880-33 

NIST Micronutrients Measurement

Quality Assurance Program

Winter, Spring, and Fall 1991 

Comparability Studies

Results for Round Robins XXI, XXII, and XXIII 

Fat-Soluble Vitamins and Carotenoids in Human Serum

Neal E. Craft (Former Employee)

David L. Duewer

Margaret C. Kline

Sam A. Margolis (Retired)

Willie E. May

Katherine E. Sharpless

Jeanice B. Thomas

This publication is available free of charge from

http://dx.doi.org/10.6028/NIST.IR.7880-33
NISTIR 7880-33

NIST Micronutrients Measurement

Quality Assurance Program

Winter, Spring, and Fall 1991 

Comparability Studies

Results for Round Robins XXI, XXII, and XXIII 

Fat-Soluble Vitamins and Carotenoids in Human Serum

Neal E. Craft (Former Employee)

David L. Duewer

Sam A. Margolis (Retired)

Jeanice B. Thomas

Chemical Sciences Division, Materials Measurement Laboratory 

Margaret C. Kline

Biomolecular Measurement Division, Materials Measurement Laboratory 

Katherine E. Sharpless 

Office of Special Programs 

Wille E. May

Under Secretary of Commerce for Standards and Technology and Director

This publication is available free of charge from

http://dx.doi.org/10.6028/NIST.IR.7880-33 

June 2015 

U.S. Department of Commerce 

Penny Pritzker, Secretary

National Institute of Standards and Technology

Willie E. May, Under Secretary of Commerce for Standards and Technology and Director
(This page intentionally blank)

ii
Abstract

The National Institute of Standards and Technology coordinates the Micronutrients Measurement 

Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins 

and carotenoids in human serum and plasma. This report describes the design of and results for the 

Winter, Spring and Fall 1991 MMQAP measurement comparability improvement studies: 1) Round 

Robin XXI Fat-Soluble Vitamins and Carotenoids in Human Serum, 2) Round Robin XXII FatSoluble Vitamins and Carotenoids in Human Serum, and 3) Round Robin XXIII Fat-Soluble 

Vitamins and Carotenoids in Human Serum. The materials for Round Robin XXI were shipped to 

participants in January 1991; participants were requested to provide their measurement results by 

March 1, 1991. The materials for Round Robin XXII were shipped to participants in May 1991; 

participants were requested to provide their measurement results by July 9, 1991. The materials for 

Round Robin XXIII were shipped to participants in August 1991; participants were requested to 

provide their measurement results by September 13, 1991. 

Keywords

Human Serum 

Retinol, α-Tocopherol, Total and Trans-β-Carotene

iii
Table of Contents 

Abstract ............................................................................................................................................... iii

Keywords ............................................................................................................................................ iii

Table of Contents ............................................................................................................................... iv

Introduction ......................................................................................................................................... 1

Round Robin XXI: Fat-Soluble Vitamins and Carotenoids in Human Serum ............................ 1

Round Robin XXII: Fat-Soluble Vitamins and Carotenoids in Human Serum .......................... 2

Round Robin XXIII: Fat-Soluble Vitamins and Carotenoids in Human Serum ........................ 2

References ............................................................................................................................................ 3

Appendix A. Shipping Package Inserts for RR21 ........................................................................ A1

Appendix B. Final Report for RR21 .............................................................................................. B1 

Appendix C. Revised “All-Lab Report” for RR21 ....................................................................... C1 

Appendix D. Shipping Package Inserts for RR22 ........................................................................ D1 

Appendix E. Final Report for RR22 .............................................................................................. E1 

Appendix F. Individualized Report for RR22 ............................................................................... F1 

Appendix G. Revised “All-Lab Report” for RR22 ....................................................................... G1 

Appendix H. Shipping Package Inserts for RR23........................................................................ H1 

Appendix I. Final Report for RR23................................................................................................. I1 

Appendix J. Revised “All-Lab Report” for RR23 ......................................................................... J1 

iv
Introduction

Beginning in 1988, the National Institute of Standards and Technology (NIST) has coordinated the 

Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure 

fat- and water-soluble vitamins and carotenoids in human serum and plasma. The MMQAP 

provides participants with measurement comparability assessment through use of interlaboratory 

studies, Standard Reference Materials (SRMs) and control materials, and methods development and 

validation. Serum-based samples with assigned values for the target analytes (retinol, alphatocopherol, gamma/beta-tocopherol, trans- and total beta-carotene, and ascorbic acid) and 

performance-evaluation standards are distributed by NIST to laboratories for analysis. 

Participants use the methodology of their choice to determine analyte content in the control and 

study materials. Participants provide their data to NIST, where it is compiled and evaluated for 

trueness relative to the NIST value, within-laboratory precision, and concordance within the 

participant community. NIST provides the participants with a technical summary report concerning 

their performance for each exercise and suggestions for methods development and refinement. 

Participants who have concerns regarding their laboratory’s performance are encouraged to consult 

with the MMQAP coordinators. 

All MMQAP interlaboratory studies consist of individual units of batch-prepared samples that are 

distributed to each participant. For historical reasons these studies are referred to as “Round 

Robins”. The MMQAP program and the nature of its studies are described elsewhere. [1] 

Round Robin XXI: Fat-Soluble Vitamins and Carotenoids in Human Serum

Participants in the MMQAP Fat-Soluble Vitamins and Carotenoids in Human Serum Round Robin 

XXI comparability study (hereafter referred to as RR21) received four lyophilized human serum test 

samples for analysis. Unless multiple vials were previously requested, participants received one vial 

of each serum. These sera were shipped on dry ice to participants in January 1991. The 

communication materials included in the sample shipment are described in Appendix A.

Participants are requested to report values for all fat-soluble vitamin-related analytes that are of 

interest to their organizations. Not all participants report values for the target analytes, and some 

participants report values for non-target analytes.

The final report delivered to every participant in RR21 is reproduced as Appendix B. This report 

included: 

• Our analysis of the participants’ results. 

• Tabular presentations of all participant results. 

• Graphical presentations of interlaboratory precision over time. These figures are no longer 

available, but would have been very similar to those in Appendix E. 

Each participant also received an “Individualized Report” that graphed their results for selected 

analytes. These figures are no longer available, but would have been very similar in format to those 

in Appendix F. 

Appendix C lists all of the measurement results reported for RR21 in a more accessible format. 

1 
Round Robin XXII: Fat-Soluble Vitamins and Carotenoids in Human Serum

Participants in the MMQAP Fat-Soluble Vitamins and Carotenoids in Human Serum Round Robin 

XXII comparability study (hereafter referred to as RR22) received three lyophilized human serum 

test samples for analysis. Unless multiple vials were previously requested, participants received one 

vial of each material. These sample materials were shipped on dry ice to participants in May 1991. 

The communication materials included in the sample shipment are described in Appendix D. 

Participants are requested to report values for all fat-soluble vitamin-related analytes that are of 

interest to their organizations. Not all participants report values for the target analytes, and some 

participants report values for non-target analytes.

The final report delivered to every participant in RR22 is reproduced as Appendix E. This report 

included: 

• Our analysis of the participants’ results.

• Tabular presentations of all participant results. 

• Graphical presentations of interlaboratory precision over time.

Each participant also received an “Individualized Report” that graphs their results for selected 

analytes. This report included graphical summaries of the participant’s results relative to the 

assigned values over the previous five years. An example of a RR22 Individualized Report is given 

in Appendix F. 

Appendix G lists all of the measurement results reported for RR22 in a more accessible format. 

Round Robin XXIII: Fat-Soluble Vitamins and Carotenoids in Human Serum

Participants in the MMQAP Fat-Soluble Vitamins and Carotenoids in Human Serum Round Robin 

XXIII comparability study (hereafter referred to as RR23) received four lyophilized human sera and 

one human serum extract in hexane analysis. Unless multiple vials were previously requested, 

participants received one vial of each material. These sample materials were shipped on dry ice to 

participants in August 1991. The communication materials included in the sample shipment are 

described in Appendix H. 

Participants are requested to report values for all fat-soluble vitamin-related analytes that are of 

interest to their organizations. Not all participants report values for the target analytes, and some 

participants report values for non-target analytes.

It is probable that a preliminary report for RR23 was mailed to all participants shortly before the 

October 19, 1991 NIST/NCI Micronutrients Analysis Workshop, however, no version of either the 

letter or the preliminary report is available. Likewise, only the tabular parts of the Final Report for 

RR23 is available; these tables are reproduced as Appendix I. 

It is probable that each participant also received an “Individualized Report” that graphically analyzes 

their results for selected analytes. While there is no longer an example of a RR23 Individualized 

Report, its design would have been similar to the RR22 example in Appendix F. 

Appendix J lists all of the measurement results reported for RR23 in a more accessible format. 

2 
References

1 Duewer DL, Brown Thomas J, Kline MC, MacCrehan WA, Schaffer R, Sharpless KE, May WE, 

Crowell JA. NIST/NCI Micronutrients Measurement Quality Assurance Program: Measurement 

Repeatabilities and Reproducibilities for Fat-Soluble Vitamin-Related Compounds in Human 

Sera. Anal Chem 1997;69(7):1406-1413. 

3 
Appendix A. Shipping Package Inserts for RR21 

Two items were included in each package shipped to an RR21 participant:

• Cover letter. The original letter has been lost. It likely would have described the four 

lyophilized sample materials (sera 144 to 147), given guidance on reconstituting these 

samples, stated that results were due March 1, 1991 and to whom they should be sent, 

and who to contact with technical questions. 

• Datasheets. The following two pages reproduce the form. The first page of the form is 

for reporting the primary-focus analytes: retinol, α-tocopherol, and total and trans-βcarotene. The second page is for reporting three “optional” analytes: retinyl palmitate, 

γ-tocopherol, and lycopene; one participant also reported α-carotene. In the absence of 

other information, the lycopene and α-carotene results are assumed to report total 

lycopene and total α-carotene.

The cover letter and datasheet were enclosed in a sealed waterproof bag along with the samples.

A1 
RR XXI JANUARY 1991

REPORT ON NIST/NCI SAMPLES FROM LABORATORY # _________ 

DATE OF ANALYSIS _____________ 

RESULTS IN μg/mL

SAMPLE # ANALYTE

SERUM 144 RETINOL

VIAL # ___ B-CAROTENE

Trans Total

A-TOCOPHEROL

SERUM 144 RETINOL

VIAL # ___ B-CAROTENE

Trans Total

A-TOCOPHEROL

SERUM 144 RETINOL

VIAL # ___ B-CAROTENE

Trans Total

A-TOCOPHEROL

SERUM 144 RETINOL

VIAL # ___ B-CAROTENE

Trans Total

A-TOCOPHEROL

 Add 1.0 mL of Distilled water to the Serum Samples

A2 
RR XXI JANUARY 1991

REPORT ON NIST/NCI SAMPLES FROM LABORATORY # _________

OPTIONAL ANALYTES

DATE OF ANALYSIS _____________ 

RESULTS IN μg/mL

SAMPLE # ANALYTE

SERUM 144 RETINYL PALMITATE

VIAL # ___ LYCOPENE

GAMMA-TOCOPHEROL

SERUM 144 RETINYL PALMITATE

VIAL # ___ LYCOPENE

Trans Total

GAMMA-TOCOPHEROL

SERUM 144 RETINYL PALMITATE

VIAL # ___ LYCOPENE

Trans Total

GAMMA-TOCOPHEROL

SERUM 144 RETINYL PALMITATE

VIAL # ___ LYCOPENE

Trans Total

GAMMA-TOCOPHEROL

A3 
Appendix B. Final Report for RR21 

The following six pages are the available components of the final report for RR21 as 

provided to all participants. The original report consisted of:

• A cover letter and discussion.

• Tables 1 to 3 that list results and various summary values for retinol,

α-tocopherol, and total β-carotene.

• Tables 4 to 7 that list results and simple summary statistics for trans-β-carotene,

retinyl palmitate, lycopene, and γ-tocopherol.

• Graphical presentations of interlaboratory precision over time for retinol,

α-tocopherol, and total β-carotene.

When the isomeric form of an analyte is not specified, it is likely that most participants 

reported “total” (the sum of all isomers.) Since resolution of γ-tocopherol and 

β-tocopherol is challenging, the results reported as γ-tocopherol can be confidently 

assumed to be γ/β-tocopherol. 

The graphical presentations for RR21 are no longer available; however, they would have 

been very similar to the graphs in the Final Report for RR22 in Appendix E. 

Due to the complex formatting used in the Tables, the originally listed laboratory codes 

have been deleted without replacement. However, Appendix C provides a complete 

listing of the RR21 results where the original codes have been altered to ensure 

confidentiality. Appendix C also provides more relevant summary statistics.

B1 
May 1, 1991 

Dear Colleague: 

NISr UNITED STATES DEPARTMENT DF COMMERCE National Institute of Standards and Technology 

Gaithersburg, Maryland 20899 

Enclosed is a packet describing both overall and your labs performance in NIST/NCI FatSoluble in Serum Analysis Round-Robin XXL Specifically, the packet contains for retinal, cxtocopherol, and ~-carotene, respectively: a tabular presentation of all data received; a 

graphical presentation of the improvement in Interlaboratory Precision vs. Time; a Control 

Chart representing your labs performance vs. the assigned value for all samples over the past 

three years; a graphical presentation of your analyses of blind-duplicate samples over the 

past three years. Tabular data only are presented for trans-~-carotene, retinyl palmitate, 

lycopene and y-tocopherol. 

Data reported for retinol, cx-tocopherol and ~-carotene are given in Tables 1-3. All values 

reported for the study are shown on the left side of section "A" of the Tables along with the 

Grand Average for all data reported. The middle or "B" section of Tables 1-3 provides the 

trimmed average, standard deviation, and an identification of the values dropped from the 

pool during the trimming process (indicated by an asterisk). Simply stated, the trimmed 

average is cletermined by iteratively dropping all values outside two standard deviations from 

the floating average. An NIST value is also provided in the "B" section of Tables 1-3 along 

with the trimmed average determined when each sample was analyzed previously. The bias 

( % ) relative to the assigned value for each sample is given in the "C" section of each Table. 

As in past studies, the trimmed average is used as the Assigned Pool Value for all three core 

analytes (retinol, cx-tocopherol, and total ~-carotene). The relatively close agreement between 

the NIST and the trimmed average allows us to continue the convention of 0-5% bias from 

the assigned value representing EXCEPTIONAL performance, 6-10% ACCEPTABLE 

performance, 11-20% MARGINAL performance, and >20% UNACCEPTABLE with respect 

to the current state-of-the-practice for measurement of these analytes in serum/plasma. 

We encourage labs that receive "U" ratings for any of the core analytes to contact us for 

discussions concerning possible sources of bias in your methodology. Given our limited 

resources, we will have to consider dropping labs that receive unacceptable ratings for three 

consecutive studies. 

Data for non-core analytes reported for RRXXI are provided in Tables 4-7. For trans-~­

carotene (Table 4), it is very encouraging that the RSD of the five reported values is less than 

10% for all four samples. Retinyl palmitate levels are very low (Table 5) for the samples used 

in this study. For the sample with the highest concentration (144), the agreement among the 

data reported by four of the five labs, RSD 22%, is quite commendable for the 50 ppb level. 

B2
May 1, 1991 

Page2 

Twelve laboratories reported data for lycopene and y-tocopherol. The RSD for y-tocopherol 

is approaching the level that we expect for the core analytes (~ 10%). For lycopene, we 

suspect that the scatter in the data may, in part, be due to partial separation of lycopene 

isomers and/or instability oflycopene reference standard solutions. We will carefully monitor 

results for these analytes and make recommendations to you at this years workshop 

(Saturday, October 26) regarding strategies for improving interlaboratory measurement 

capabilities/data. 

Round-Robin XXII samples will be shipped to you on May 14. Results are required by July 

9. 

Sincerely , 

~ l,-,r? {]'..J ~#·\, , __ r -· \ . '=-- ~ 

Willie E. May, Ph.D. ~ 

Chief 

Organic Analytical Research Division 

Chemical Science and Technology Laboratory 

Enclosures 

/( .u1 [ r;,;;-

Neal E. Craft 

Biologist 

Organic Analytical Research Division 

Chemical Science Technology Laboratory 

B3
Table 1. A B c 

Rol.l'ld Robin XXI Round Robin XXI 

Retinol Results Retinol Results Percent Bias from Assigned Pool Value. 

Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # 

Lab # 144 145 146 147 Lab # 144 145 146 147 144 145 146 147 

0.934 0.483 0.468 0.253 0.934 0.483 0.468 0.253 -2.5 ·1.4 ·8.9 ·3.3 0.995 0.525 0.544 0.273 0.995 0.525 0.544 0.273 3.9 7.2 5.9 4.3 

1.086 0.518 0.533 0.279 1.086 0.518 0.533 0.279 13.4 5.8 3.8 6.6 

1.062 0.521 0.592 0.299 1.062 0.521 0.592 0.299 10.9 6.4 15.3 14.2 

0.960 0.507 0.529 0.277 0.960 0.507 0.529 0.277 0.2 3.4 3.0 5.8 

0.932 0.507 0.552 0.273 0.932 0.507 0.552 0.273 ·2.7 3.5 7.5 4.3 

1.060 0.480 0.523 0.259 1.060 0.480 0.523 0.259 10.7 ·2.0 1.8 ·1.1 

0.892 0.470 0.505 0.226 0.892 0.470 0.505 0.226 ·6.9 ·4.0 · 1.7 ·13.7 

0.910 0.494 0.492 0.258 0.910 0.494 0.492 0.258 ·5.0 0.9 ·4.2 ·1.4 

0.951 0.468 0.500 0.263 0.951 0.468 0.500 0.263 ·0.7 ·4.4 ·2.6 0.5 

0.920 0.510 0.500 0.260 0.920 0.510 0.500 0.260 ·4.0 4.1 ·2.6 ·0.7 

0.955 0.499 0.561 0.281 0.955 0.499 0.561 0.281 ·0.3 1.9 9.2 7.3 

0.989 0.506 0.531 0.274 0.989 0.506 0.531 0.274 3.2 3.3 3.4 4.7 

1.115 0.421 0.412 0.214 *1.115 0.421 0.412 0.214 16.4 • 14.1 ·19.7 · 18.3 

0.890 0.483 0.472 0.261 0.890 0.483 0.472 0.261 ·7.1 ·1.4 ·8.1 ·0.3 

0.868 0.446 0.435 0.224 0.868 0.446 0.435 0.224 ·9.4 ·8.9 ·15.3 ·14.4 

0.960 0.500 0.500 0.250 0.960 0.500 0.500 0.250 0.2 2.1 ·2.6 ·4.5 

0.770 0.430 0.440 0.210 *0.770 0.430 0.440 0.210 ·19.6 ·12.2 ·14.3 ·19.8 

1.145 0.599 0.610 0.315 *1.145 *0.599 0.610 0.315 19.5 22.3 18.8 20.3 u 

0.759 0.422 0.440 0.202 *0.759 0.422 0.440 *0.202 ·20.8 ·13.8 -14.3 ·22.8 u 0.902 0.425 0.446 0.236 0.902 0.425 0.446 0.236 ·5.8 . ·13.2 · 13.1 ·9.8 0.965 0.492 0.499 0.261 0.965 0.492 0.499 0.261 0.7 0.5 ·2.8 ·0.3 0.978 0.311 0.565 0.540 0.978 *0.311 0.565 *0.540 2.1 ·36.5 10.0 106.3 u 

1.014 0.547 0.562 0.278 1.014 0.547 0.562 0.278 5.9 11.7 9.4 6.2 

0.904 0.463 0.507 0.252 0.904 0.463 0.507 0.252 ·5.6 ·5.5 • 1.3 ·3.7 

1.049 0.602 0.601 0.299 1.049 *0.602 0.601 0.299 9.5 22.9 17.0 14.2 

1.003 0.572 0.554 0.295 1.003 0.572 0.554 0.295 4.7 16.8 7.9 12.7 

0.988 0.550 0.489 0.248 0.988 0.550 0.489 0.248 3.1 12.3 ·4.8 ·5.3 

0.968 0.565 0.639 0.302 0.968 0.565 0.639 0.302 1.1 15.4 24.4 15.4 

0.959 0.509 0.584 0.237 0.959 0.509 0.584 0.237 0.1 3.9 13.7 ·9.5 

0.840 0.472 0.485 0.277 0.840 0.472 0.485 0.277 ·12.3 ·3.6 ·5.6 5.6 

1.580 0.830 0.860 0.460 *1.580 *0.830 *0.860 *0.460 64.9 69.5 67.5 75.7 u 

0.897 0.494 0.488 0.260 0.897 0.494 0.488 0.260 ·6.4 0.9 ·5.0 ·0.7 NIST 0.921 0.488 0.520 0.254 

Grand AVG 0.976 0.504 0.528 0.276 TRIMMED AVG 0.958 0.490 0.514 0.262 

SD 0.138 0.081 0.081 0.065 SD 0.062 0.038 0.051 0.025 

RSD 14.2 16.1 15.3 23.4 RSD 6.5 7.8 10.0 9.7 

PREY TRIMMED AVG 0.921 0.485 0.515 0.259 

Grand AVG = all values included. DATE 1/88 1/90 5/90 1/90 

L = Late results not included in the statistics TRIMMED AVG = the mean of the values that fall within + or • 2 standard deviations. 

All concentrations are reported in ug/ml. * = Value fOLl'ICI to be outside + or · 2 standard deviations of the average. 

B4
Table 2. A B c 

Round Robin XXI Rol.l'ld Robin XXI 

Alpha·Tocopherol Results Alpha·Tocopherol Results Percent Bias from Assigned Pool Value. 

Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # 

Lab # 144 145 146 147 Lab # 144 145 146 147 144 145 146 147 

10.54 6.72 6.68 4.84 10.54 6.72 6.68 4.84 • 1.3 ·2.7 1.2 ·0.9 10.72 6.51 6.21 4.52 10.72 6.51 6.21 4.52 0.4 ·5.7 ·5.8 ·7.5 

10.91 7.22 6.90 5.10 10.91 7.22 6.90 5.10 2.2 4.5 4.6 4.4 

11.81 7.20 7.95 5.51 11.81 7.20 *7.95 5.51 10.6 4.3 20.6 12.8 

11.23 7.09 6.71 4.99 11.23 7.09 6.71 4.99 5.1 2.7 1.8 2.2 

11.10 6.80 6.90 5.20 11.10 6.80 6.90 5.20 3.9 ·1.5 4.6 6.5 

11.00 6.70 6.60 5.10 11.00 6.70 6.60 5.10 3.0 ·3.0 0.1 4.4 

10.53 6.80 6.69 4.35 10.53 6.80 6.69 4.35 ·1.4 ·1.5 1.5 ·10.9 

8.95 5.85 5.65 4.15 *8.95 *5.85 5.65 4.15 ·16.2 ·15.3 ·14.4 ·15.0 

9.84 6.84 6.55 4.89 9.84 6.84 6.55 4.89 ·7.9 ·1.0 ·0.7 0.1 

10.37 6.87 6.14 4.79 10.37 6.87 6.14 4.79 ·2.9 ·0.5 ·6.9 ·1.9 

10.99 7.33 6.49 5.16 10.99 7.33 6.49 5.16 2.9 6.1 ·1.6 5.6 

10.30 6.70 6.63 4.75 10.30 6.70 6.63 4.75 ·3.6 ·3.0 0.5 ·2.8 

10.49 6.65 6.62 4.87 10.49 6.65 6.62 4.87 ·1.8 ·3.7 0.4 ·0.3 12.86 6.71 3.96 3.75 12.86 6.71 *3.96 *3.75 20.4 ·2.9 ·40.0 ·23.2 u 10.92 7.03 6.79 5.23 10.92 7.03 6.79 5.23 2.2 1.8 3.0 7.0 

11.47 7.43 6.66 4.51 11.47 7.43 6.66 4.51 7.4 7.6 1.0 ·7.7 

9.86 6.73 6.93 4.99 9.86 6.73 6.93 4.99 ·7.7 ·2.6 5.1 2.2 

10.70 6.89 6.44 4.92 10.70 6.89 6.44 4.92 0.2 ·0.2 ·2.3 0.7 

10.16 7.63 6.95 5.67 10.16 *7.63 6.95 5.67 ·4.9 10.5 5.4 16.1 

11.00 7.17 6.82 5.50 11.00 7.17 6.82 5.50 3.0 3.8 3.4 12.6 

13.48 7.90 7.43 4.62 *13.48 *7.90 7.43 4.62 26.2 14.4 12.7 ·5.4 

10.53 7.10 7.28 5.57 10.53 7.10 7.28 5.57 ·1.4 2.8 10.4 14.0 

10.69 7.14 6.71 5.10 10.69 7.14 6.71 5.10 0.1 3.4 1.8 4.5 

10.50 5.00 7.50 7.00 10.50 *5.00 7.50 *7.00 ·1.7 ·27.6 13.7 43.3 u 

10.92 6.70 6.38 4.70 10.92 6.70 6.38 4.70 2.2 ·3.0 ·3.2 ·3.8 10.10 6.13 5.96 4.76 10.10 *6.13 5.96 4.76 ·5.4 ·11.2 ·9.6 ·2.5 10.70 6.90 6.90 5.20 10.70 6.90 6.90 5.20 0.2 ·0.1 4.6 6.5 

10.32 7.77 5.94 4.06 10.32 *7.77 5.94 4.06 ·3.4 12.5 ·9.9 ·17.0 

12.06 7.85 8.62 6.24 12.06 7.85 8.62 6.24 12.9 13.7 30.7 27.8 u 

10.00 7.11 6.77 5.03 10.00 7.11 6.77 5.03 ·6.4 2.9 2.7 3.0 10.50 6.63 6.17 4.47 10.50 6.63 6.17 4.47 ·1.7 ·4.0 ·6.5 ·8.6 

9.30 5.97 5.79 4.19 9.30 *5.97 5.79 4.19 ·12.9 ·13.6 ·12.2 ·14.2 

10.71 6.60 6.23 4.69 10.71 6.60 6.23 4.69 0.3 ·4.4 ·5.5 ·4.0 NIST 10.40 6.55 6.29 4.55 

Grand AVG 10.751 6.872 6.616 4.953 TRIMMED AVG 10.679 6.906 6.594 4.884 

SD 0.872 0.574 0.752 SD 0.645 0.249 0.440 0.411 

RSD 8.1 8.4 11.4 12.4 RSD 6.0 3.6 6.7 8.4 

PREY TRllltED AVG 10.54 6.68 6.65 4.83 

Grand AVG = all values included. 

DATE 1/88 1/90 5/90 1/90 

L = Late results not included in the statistics TRIMMED AVG = the me., of the values that fall within + or • 2 standard deviations. 

All concentrations are reported in ug/mL. * = Value fol.l'ld to be outside + or • 2 standard deviations of the average. 

B5
Table 3. A B c 

Round Robin XXI Round Robin XXI 

Total Beta·Carotene Results Total Beta·Carotene Results Percent Bias from Assigned Pool Value. 

Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # 

Lab # 144 145 146 147 Lab # 144 145 146 147 144 145 146 147 

0.855 1.077 0.190 0.422 0.855 1.077 0.190 0.422 o.a ·1.1 ·2.5 ·0.1 

0.786 0.921 0.172 0.373 0.786 0.921 0.172 0.373 ·7.3 ·15.4 ·11.7 ·11.7 

0.826 1.004 0.186 0.435 0.826 1.004 0.186 0.435 ·2.6 ;7.8 ·4.5 3.0 

0.895 1.223 0.209 0.477 0.895 1.223 0.209 0.477 5.5 12.3 7.3 13.0 

1.130 1.516 0.259 0.550 *1.130 *1.516 *0.259 *0.550 33.3 39.3 32.9 30.3 u 

0.802 1.163 0.204 0.474 0.802 1.163 0.204 0.474 ·5.4 6.8 4.7 12.3 

0.882 1.138 0.136 0.384 0.882 1.138 *0.136 0.384 4.0 4.5 ·30.2 ·9.1 

0.880 1.070 0.200 0.430 0.880 1.070 0.200 0.430 3.8 • 1.7 2.7 1.8 

0.895 1.027 0.191 0.434 0.895 1.027 0.191 0.434 5.5 ·5.7 ·2.0 2.8 

0.831 1.046 0.177 0.389 0.831 1.046 0.177 0.389 ·2.0 ·3.9 ·9.1 ·7.9 

0.795 1.107 0.176 0.442 0.795 1.107 0.176 0.442 ·6.2 1.7 ·9.7 4.7 

0.818 1.188 0.193 0.443 0.818 1.188 0.193 0.443 ·3.5 9.1 ·0.9 4.9 

0.860 1.020 0.230 0.430 0.860 1.020 0.230 0.430 1.4 ·6.3 18.1 1.8 

0.738 0.911 0.186 0.351 *0.738 0.911 0.186 0.351 ·13.0 ·16.3 ·4.5 ·16.9 

0.870 1.204 0.204 0.448 0.870 1.204 0.204 0.448 2.6 10.6 4.7 6.1 

0.833 1.110 0.214 0.558 0.833 1.110 0.214 *0.558 -1.8 2.0 9.8 32.2 

0.372 0.467 0.082 0.199 *0.372 *0.467 *0.082 *0.199 -56.1 ·57.1 -57.9 ·52.9 u 

0.980 1.130 0.221 0.495 *0.980 1.130 0.221 0.495 15.6 3.8 13.4 17.2 

0.918 1.200 0.168 0.403 0.918 1.200 0.168 0.403 8.3 10.2 ·13.8 ·4.6 

0.601 1.290 0.296 0.561 0.601 1.290 0.296 0.561 ·29.1 18.5 51.9 32.9 u 

1.650 2.360 0.527 0.905 *1.650 *2.360 *0.527 *0.905 94.6 116.8 170.5 114.3 u 0.854 1.065 0.196 0.401 0.854 1.065 0.196 0.401 0.7 ·2.2 0.5 ·5.0 0.816 1.079 0.190 0.369 0.816 1.079 0.190 0.369 -3.8 -0.9 -2.5 ·12.6 

NIST 0.801 1.040 0.199 0.402 

Grand AVG 0.865 1.144 0.209 0.451 TRIMMED AVG 0.848 1.089 0.195 0.422 

SD 0.219 0.323 0.080 0.125 SD 0.039 0.089 0.017 0.040 

RSD 25.4 28.2 38.3 27.7 RSD 4.6 8.2 8.6 9.4 

Grand AVG = all values included. PREY TRIMMED AVG 0.796 1.038 0.189 0.409 

L = Late results not included in the statistics DATE 1/88 1/90 5/90 1/90 

All concentrations are reported in ug/mL. TRIMMED AVG = the mean of the values that fall within + or • 2 staidard deviations. 

* = Value found to be outside + or • 2 standard deviations of the average. 

B6
Table ... Table 5. 

Round Robin XXI Round Robin XXI 

Trans Beta-Carotene Results Retinyl Palmitate 

Serum # Serum # Serum # Serum # Serum # serum # Serum # Serum # 

Lab # 144 145 146 147 Lab # 144 145 146 147 

o.n9 0.986 0.169 0.363 0.045 0.014 

0.744 1.045 0.170 0.415 0.059 

0.730 1.075 0.172 0.396 0.038 0.027 0.010 0.012 

0.687 0.849 o.1n 0.325 0.039 

NIST 0.723 0.950 0.179 0.360 0.166 0.172 0.068 0.097 

Grand AVG 0.733 0.981 0.173 0.372 Grand AVG 0.069 0.099 0.031 0.055 

SD 0.033 0.089 0.004 0.035 SD 0.055 0.032 

RSD 4.6 9.0 2.5 9.4 RSD 78.9 105.4 

TRIMMED AVG 0.045 0.027 0.012 0.012 

SD 0.010 0.003 

RSD 21.7 22.9 

* = all values removed 

Table 6. Table 7. 

Round Robil"! XXI Round Robin XXI 

Lycopene G811111a Tocopherol 

Serum # serum # Serum # Serum # Serum # Serum # Serum # Serum # 

Lab # 144 145 146 147 Lab # 144 145 146 147 

0.275 0.388 0.247 0.281 2.40 0.90 2.50 1.90 

0.178 0.247 0.146 0.193 3.25 3.64 2.52 0.256 0.360 0.211 0.270 2.50 0.91 2.51 2.22 

0.192 0.258 0.120 0.282 2.99 1.20 3.00 2.53 0.140 0.218 0.133 0.154 3.11 1.11 2.92 2.53 

0.279 0.447 0.243 0.341 5.21 2.02 5.22 4.20 

0.259 0.420 0.217 0.307 2.75 0.98 2.68 2.26 

0.317 0.449 0.283 0.315 2.87 1.23 2.90 2.50 

0.197 0.267 0.166 0.215 2.60 1.33 2.87 2.53 

0.360 0.515 0.317 0.450 2.51 1.01 2.66 2.24 

0.128 0.198 0.120 0.154 2.57 1.38 2.65 2.44 

1.660 1.570 1.670 1.630 3.29 1.00 3.18 2.49 NIST 2.58 0.79 2.65 2.01 

Grand AVG 0.353 0.445 0.323 0.383 Grand AVG 2.971 1.154 3.029 2.490 

SD 0.417 0.369 0.429 0.402 SD 0.735 0.327 0.727 0.555 

RSD 118.1 83.0 133.0 105.0 RSD 24.7 . 28.3 24.0 22.3 

TRIMMED AVG 0.235 0.342 0.200 0.269 TRIMMED AVG 2.803 1.104 2.864 2.378 

SD 0.073 0.109 0.068 0.088 SD 0.318 0.173 0.332 0.202 

RSD 31.3 31.8 34.0 32.6 RSD 11.3 15.7 11.6 8.5 

* = all values removed * = all values removed All concentrations are reported in ug/mL. 

B7
Appendix C. Updated “All-Lab Report” for RR21

The following five pages are a revised version of an “All-Lab” report for RR21. This 

report has three parts: 

• pages 1 to 3 list results for all analytes reported at least twice, counting both

participants and NIST analysts.

• page 4 provides a legend for pages 1 thru 3.

• page 5 summarizes each participant’s performance for retinol, α-tocopherol, and 

total β-carotene. These summaries are compatible with the percent bias 

evaluation advice given in the RR21 Report. However, the current bias 

summaries are estimated relative to the median of all reported values for each 

analyte in each serum rather than to the “Trimmed Average” used in the original 

and detailed in Appendix B. These original reference values were estimated from 

on-time results, with subjective exclusion of results deemed non-representative.

To ensure confidentiality, the laboratory identifiers used in this “All-Lab Report” have 

been altered from those used in RR21. The only attributed results are those reported by 

NIST. The NIST results are not used in the assessment of the consensus summary results 

of the study. 

Note: The results designated NISTc in this updated All-Lab report are designated as 

NIST in the Tables of Appendix B. 

C1 
Round Robin XXI Laboratory Results

All Lab Report Page 1 / 5

Retinyl Palmitate α-Tocopherol γ/β-Tocopherol

Lab 144 145 146 147 144 145 146 147 144 145 146 147 144 145 146 147

FSV-BA 0.989 0.506 0.531 0.274 0.038 0.027 0.010 0.012 10.49 6.65 6.62 4.87 5.21 2.02 5.22 4.20

FSV-BD 0.932 0.507 0.552 0.273 11.10 6.80 6.90 5.20

FSV-BE 0.951 0.468 0.500 0.263 10.37 6.87 6.14 4.79

FSV-BF 0.892 0.470 0.505 0.226 10.53 6.80 6.69 4.35 2.40 0.90 2.50 1.90

FSV-BH 0.890 0.483 0.472 0.261 0.039 nd nd nd 10.92 7.03 6.79 5.23 2.50 0.91 2.51 2.22

FSV-BI 0.995 0.525 0.544 0.273 0.059 nd nd nd 10.72 6.51 6.21 4.52 2.87 1.23 2.90 2.50

FSV-BJ 0.965 0.492 0.499 0.261 10.69 7.14 6.71 5.10 3.11 1.11 2.92 2.53

FSV-BK 0.978 0.311 0.565 0.540 10.50 5.00 7.50 7.00

FSV-BL 1.145 0.599 0.610 0.315 11.00 7.17 6.82 5.50

FSV-BM 0.902 0.425 0.446 0.236 10.53 7.10 7.28 5.57

FSV-BY 0.934 0.483 0.468 0.253 0.045 nd 0.014 nd 10.54 6.72 6.68 4.84 2.99 1.20 3.00 2.53

FSV-BZ 9.86 6.73 6.93 4.99

FSV-CA 1.062 0.521 0.592 0.299 11.81 7.20 7.95 5.51

FSV-CH 0.968 0.565 0.639 0.302 12.06 7.85 8.62 6.24

FSV-CJ 1.086 0.518 0.533 0.279 10.91 7.22 6.90 5.10

FSV-CK 0.868 0.446 0.435 0.224 11.47 7.43 6.66 4.51

FSV-CM 10.50 7.10 7.30 5.90

FSV-CO 0.960 0.507 0.529 0.277 11.23 7.09 6.71 4.99

FSV-CP

FSV-CQ 0.910 0.494 0.492 0.258 8.95 5.85 5.65 4.15

FSV-CV 0.904 0.463 0.507 0.252 10.10 6.13 5.96 4.76 2.57 1.38 2.65 2.44

FSV-CY 0.960 0.500 0.500 0.250 10.70 6.89 6.44 4.92

FSV-DC 0.955 0.499 0.561 0.281 10.30 6.70 6.63 4.75 2.75 0.98 2.68 2.26

FSV-DE 9.84 6.84 6.55 4.89 2.51 1.01 2.66 2.24

FSV-DG 0.920 0.510 0.500 0.260 10.99 7.33 6.49 5.16

FSV-DH 0.759 0.422 0.440 0.202 0.166 0.172 0.068 0.097 13.48 7.90 7.43 4.62 3.29 1.00 3.18 2.49

FSV-DL 0.988 0.550 0.489 0.248

FSV-DM 1.580 0.830 0.860 0.460 9.30 5.97 5.79 4.19

FSV-DN 1.060 0.480 0.523 0.259 11.00 6.70 6.60 5.10

FSV-DY 0.540 0.514 0.907 0.279

FSV-DZ 1.115 0.421 0.412 0.214 12.86 6.71 3.96 3.75

FSV-EB 1.003 0.572 0.554 0.295 10.32 7.77 5.94 4.06

FSV-EF 0.770 0.430 0.440 0.210 10.16 7.63 6.95 5.67 2.60 1.33 2.87 2.53

FSV-EJ 0.957 0.509 0.584 0.237 10.00 7.11 6.77 5.03 3.25 na 3.64 2.52

FSV-EV 1.049 0.602 0.601 0.299 10.70 6.90 6.90 5.20

FSV-EY 1.014 0.547 0.562 0.278 10.92 6.70 6.38 4.70

FSV-FL 0.897 0.494 0.488 0.260 10.71 6.60 6.23 4.69

FSV-FR 0.840 0.472 0.485 0.277 10.50 6.63 6.17 4.47

n 34 34 34 34 5 2 3 2 35 35 35 35 12 11 12 12

Min 0.540 0.311 0.412 0.202 0.038 0.027 0.010 0.012 8.95 5.00 3.96 3.75 2.40 0.90 2.50 1.90

Mean 0.963 0.504 0.539 0.276 0.069 0.099 0.031 0.055 10.74 6.88 6.64 4.98 3.00 1.19 3.06 2.53

Max 1.580 0.830 0.907 0.540 0.166 0.172 0.068 0.097 13.48 7.90 8.62 7.00 5.21 2.02 5.22 4.20

SD 0.155 0.080 0.103 0.064 0.055 0.103 0.032 0.060 0.86 0.57 0.75 0.63 0.76 0.32 0.75 0.56

CV 10 10 11 12 33 60 48 62 6 7 9 9 15 16 14 13

NISTc 0.921 0.488 0.520 0.254 10.40 6.55 6.29 4.55 2.58 0.79 2.65 2.01

Median 0.958 0.500 0.515 0.262 0.045 10.69 6.87 6.68 4.92 2.81 1.11 2.89 2.50

eSD 0.082 0.039 0.061 0.024 0.011 0.46 0.36 0.38 0.42 0.44 0.20 0.34 0.07

eCV 9 8 12 9 24 4 5 6 8 16 18 12 3

Total Retinol

C2 
Round Robin XXI Laboratory Results

All Lab Report Page 2 / 5

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BY

FSV-BZ

FSV-CA

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CQ

FSV-CV

FSV-CY

FSV-DC

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DN

FSV-DY

FSV-DZ

FSV-EB

FSV-EF

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FR

n

Min

Mean

Max

SD

CV

NISTc

Median

eSD

eCV

Total β-Carotene trans-β-Carotene Total cis-β-Carotene

144 145 146 147 144 145 146 147 144 145 146 147

0.831 1.046 0.177 0.389 0.779 0.986 0.169 0.363 0.052 0.060 0.008 0.026

0.802 1.163 0.204 0.474

0.795 1.107 0.176 0.442 0.744 1.045 0.170 0.415 0.051 0.062 0.006 0.027

0.786 0.921 0.172 0.373

0.870 1.204 0.204 0.448

0.855 1.077 0.190 0.422

0.601 1.290 0.296 0.561

0.826 1.004 0.186 0.435

0.818 1.188 0.193 0.443 0.730 1.075 0.172 0.396 0.088 0.113 0.021 0.047

0.895 1.223 0.209 0.477

0.372 0.467 0.082 0.199

0.882 1.138 0.136 0.384

0.833 1.110 0.214 0.558

0.860 1.020 0.230 0.430

0.895 1.027 0.191 0.434

0.880 1.070 0.200 0.430

0.738 0.911 0.186 0.351 0.687 0.849 0.177 0.325 0.051 0.062 0.009 0.026

0.918 1.200 0.168 0.403

1.130 1.516 0.259 0.550

0.864 1.131 0.188 0.419

0.980 1.130 0.221 0.495

1.650 2.360 0.527 0.905

0.816 1.079 0.190 0.369

0.854 1.065 0.196 0.401

24 24 24 24 4 4 4 4 4 4 4 4

0.372 0.467 0.082 0.199 0.687 0.849 0.169 0.325 0.051 0.060 0.006 0.026

0.865 1.144 0.208 0.450 0.735 0.989 0.172 0.375 0.061 0.074 0.011 0.032

1.650 2.360 0.527 0.905 0.779 1.075 0.177 0.415 0.088 0.113 0.021 0.047

0.214 0.316 0.078 0.123 0.038 0.100 0.004 0.040 0.018 0.026 0.007 0.010

13 13 15 14 5 9 2 10 21 23 32 22

0.801 1.040 0.199 0.402 0.723 0.950 0.179 0.360 0.078 0.090 0.020 0.042

0.855 1.109 0.192 0.432

0.058 0.119 0.023 0.063

7 11 12 15

C3 
Round Robin XXI Laboratory Results

All Lab Report Page 3 / 5

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BY

FSV-BZ

FSV-CA

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CQ

FSV-CV

FSV-CY

FSV-DC

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DN

FSV-DY

FSV-DZ

FSV-EB

FSV-EF

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FRn

Min

Mean

Max

SD

CV

NISTc

Median

eSD

eCV

Total Lycopene

144 145 146 147

0.140 0.218 0.133 0.154

0.256 0.360 0.211 0.270

0.279 0.447 0.243 0.341

0.178 0.247 0.146 0.193

0.197 0.267 0.166 0.215

0.275 0.388 0.247 0.281

0.259 0.420 0.217 0.307

0.128 0.178 0.120 0.154

0.360 0.515 0.317 0.450

0.192 0.258 0.120 0.282

0.317 0.449 0.283 0.315

0.182 0.301 0.231 0.231

1.660 1.570 1.670 1.630

13 13 13 13

0.128 0.178 0.120 0.154

0.340 0.432 0.316 0.371

1.660 1.570 1.670 1.630

0.402 0.357 0.412 0.387

24 23 25 24

0.256 0.360 0.217 0.281

0.095 0.138 0.098 0.089

37 38 45 32

C4
Round Robin XXI Laboratory Results

All Lab Report Page 4 / 5

Analytes Reported By One Laboratory

Values in μg/mL

Analyte Code 144 145 146 147

Total α-Carotene FSV-BJ 0.028 0.026 0.011 0.016

Symbol

na 

nd 

italics Value calculated from reported results

n 

Min

Mean Average over all non-NIST reported values.

Max

SD

CV

Median 

eSD 

eCV 

Table Legend

Interpretation

Not available (not reported by participant)

Not detected (not reported or reported as '0', 'not determined', etc.)

Number of non-NIST laboratories reporting quantitative results

Minimum non-NIST reported value.

Maximum non-NIST reported value.

Standard deviation over all non-NIST values.

Coefficient of Variation (% relative standard deviation): 100*SD/Mean

Median over all non-NIST reported values

Robust estimate of SD based on the adjusted median absolute difference from 

the median (MADe)

Robust estimate of CV, 100*eSD/Median

C5 
Round Robin XXI Laboratory Results

All Lab Report Page 5 / 5

Lab TR aT bC Label Definition

FSV-BA 3±1 -2±1 -7±3 Lab Participant code

FSV-BD 3±4 3±3 TR Total Retinol

FSV-BE -2±3 -3±3 aT a-Tocopherol

FSV-BF -7±5 -3±5 4±7 bC Total or trans-b-Carotene

FSV-BH -5±4 3±2 -3±5 % Bias (Mean ± SD) of individual serum biases

FSV-BI 5±1 -5±4 -12±4

FSV-BJ -1±2 2±2 5±3 Mean Average of (xi

-Mediani)/Mediani

FSV-BK 20±61 6±29 SD Standard deviation of (xi

-Mediani)/Mediani

FSV-BL 20±1 5±4 xi Result for analyte in serumi

FSV-BM -11±4 6±6 Mediani Median of non-NIST results in serumi

FSV-BY -5±3 -1±1 -2±1

FSV-BZ -1±5

FSV-CA 11±5 12±6

FSV-CH 13±10 21±8 18±35

FSV-CJ 7±5 4±1 -4±4

FSV-CK -13±3 2±8 1±5

FSV-CM 8±9

FSV-CO 2±2 3±2 9±3

FSV-CP -56±2

FSV-CQ -3±2 -16±1 -9±15

FSV-CV -5±2 -8±4 10±14

FSV-CY -2±2 -1±2 3±12

FSV-DC 4±5 -3±1 -1±5

FSV-DE -3±4

FSV-DG -1±3 3±4 1±3

FSV-DH -18±4 12±13 -13±7

FSV-DL 1±7 -1±10

FSV-DM 68±5 -14±1

FSV-DN 2±6 1±3 33±4

FSV-DY 10±49 0±2

FSV-DZ -9±17 -12±26

FSV-EB 10±5 -5±13 12±6

FSV-EF -17±3 6±9

FSV-EJ 1±9 0±4 122±36

FSV-EV 15±5 2±3

FSV-EY 8±2 -2±3

FSV-FL -3±3 -4±3 -6±6

FSV-FR -5±7 -6±3 -2±4

NISTc -2±2 -5±2 -4±5

%Bias Summary

The original analysis listed % Bias for each result for 

each serum calculated relative to the "Trimmed Core 

Lab Average" of that analyte in the serum. The 

summary values reported here are the (arithmetic mean 

± standard deviation) of each laboratory's reported 

results for the analyte estimated relative to each serum's 

median value.

C6
Appendix D. Shipping Package Inserts for RR22 

Two items were included in each package shipped to an RR22 participant:

• Cover letter. The original letter has been lost. It likely would have described the three 

lyophilized sample materials (sera 148 to 150), given guidance on reconstituting these 

lyophilized samples, stated that results were due July 9, 1991 and to whom they should 

be sent, and who to contact with technical questions.

• Datasheet. The following page reproduces the form. 

The cover letter and datasheet were enclosed in a sealed waterproof bag along with the samples.

D1 
ROUND ROBIN XXIVI RESULTS FROM LABORATORY # _________ 

DATE OF ANALYSIS _____________ 

RESULTS IN ug/mL

SAMPLE # ANALYTE RESULT

SERUM 148 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

SERUM 149 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

SERUM 150 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

OPTIONAL ANALYTES : SUPPLY ONE RESULT IF AVAILABLE

SERUM # 148 149 150

TRANS-BETA-CAROTENE ______ ______ ______ 

ALPHA-CAROTENE ______ ______ ______ 

RETINYL PALMITATE ______ ______ ______ 

GAMMA-TOCOPHEROL ______ ______ ______ 

LYCOPENE (TOTAL) ______ ______ ______ 

9-CIS-BETA-CAROTENE ______ ______ ______ 

13-CIS-BETA-CAROTENE ______ ______ ______ 

LUTEIN ______ ______ ______ 

ZEAXANTHIN ______ ______ ______ 

BETA-CRYPTOXANTHIN ______ ______ ______ 

 RECONSTITUTE WITH 1.0 mL of WATER.

D2 
Appendix E. Final Report for RR22 

The following nine pages are the final report for RR22 as provided to all participants: 

• A cover letter and discussion.

• Tables 1 to 3 that list the results and various summary values for retinol,

α-tocopherol, and total β-carotene.

• A page of unnumbered tables that list the results and simple summary statistics

for trans-β-carotene, α-carotene, retinyl palmitate, γ-tocopherol, total lycopene,

lutein, zeaxanthin, lutein & zeaxanthin, and β-cryptoxanthin.

• Graphical presentations of interlaboratory precision over time for retinol,

α-tocopherol, and total β-carotene.

When the isomeric form of an analyte is not specified, it is likely that most participants 

reported “total” (the sum of all isomers.) Since resolution of γ-tocopherol and 

β-tocopherol is challenging, the results reported as γ-tocopherol can be confidently 

assumed to be γ/β-tocopherol. 

Due to the complex formatting used in the Tables, the originally listed laboratory codes 

have been deleted without replacement. However, Appendix G provides a complete 

listing of the RR22 results where the original codes have been altered to ensure 

confidentiality. Appendix G also provides more relevant summary statistics.

E1 
E2 
E3
E4
E5 
E6 
E7
E8
E9
E10 
Appendix F. Representative “Individualized Report” for RR22 

Each participant in RR22 received graphical summaries of their own measurement 

performance for total retinol, α-tocopherol, and/or total β-carotene. In RR22, one set of 

graphs was prepared: 

• “Percent Bias” relative to the “Assigned Value” (the “Trimmed Average” of the

Tables in Appendix E) for of the serum-based samples distributed from 7/1987 

through 5/91. 

The following three pages constitute the individualized report for participant FSV-BA. 

F1 
FSV-BA

F2
FSV-BA

F3
FSV-BA

F4
Appendix G. Updated “All-Lab Report” for RR22 

The following five pages are an updated version of an “All-Lab” report for RR22. This 

report has three parts: 

• pages 1 and 3 list results for all analytes reported at least twice, counting both

participants and NIST analysts.

• page 4 provides a legend for pages 1 thru 3.

• page 5 summarizes each participant’s performance for retinol, α-tocopherol, and 

total β-carotene. These summaries are compatible with the percent bias 

evaluation advice given in the RR22 Report. However, the current bias 

summaries are estimated relative to the median of all reported values for each 

analyte in each serum rather than to the “Trimmed Average” used in the original 

and detailed in Appendix E. These original reference values were estimated from 

on-time results, with subjective exclusion of results deemed non-representative.

To ensure confidentiality, the laboratory identifiers used in this “All-Lab Report” have 

been altered from those used in RR22. The only attributed results are those reported by 

NIST. The NIST results are not used in the assessment of the consensus summary results 

of the study. 

Note: The results designated NISTa, NISTb, and NISTc in this updated All-Lab report

are designated NIST-1, NIST-3, and NIST-2 in the Tables of Appendix E. 

G1 
Round Robin XXII Laboratory Results

All Lab Report Page 1 / 5

Total Retinol Retinyl Palmitate α-Tocopherol γ/β-Tocopherol

Lab 148 149 150 148 149 150 148 149 150 148 149 150

FSV-BA 0.197 0.508 0.657 0.003 0.032 0.042 4.31 10.18 15.88 0.86 2.72 3.73

FSV-BD 0.204 0.522 0.704 5.15 10.90 17.11

FSV-BE 0.193 0.500 0.679 4.44 10.60 16.68

FSV-BF 0.198 0.538 0.726 4.58 10.73 16.68 0.91 3.17 4.33

FSV-BG 0.185 0.495 0.678 nd na 0.034 4.95 10.34 17.48

FSV-BH 0.178 0.478 0.638 4.76 10.38 16.11 0.94 3.12 4.33

FSV-BI 0.179 0.488 0.650 4.47 10.23 16.96 0.88 2.89 4.13

FSV-BJ 0.184 0.481 0.665 0.163 0.212 0.288 5.15 10.69 16.34 0.96 3.08 4.20

FSV-BK 0.272 0.570 0.693 5.05 10.27 16.03

FSV-BL 0.230 0.557 0.703 5.34 10.86 16.90

FSV-BM 0.190 0.497 0.658 4.60 10.40 16.60

FSV-BN 0.199 0.530 0.628 5.09 11.05 16.67 0.95 2.40 3.26

FSV-BP 0.506 0.690 0.719 7.81 14.49 19.71

FSV-BS 0.258 0.377 0.552

FSV-BY 0.183 0.472 0.636 0.009 0.019 0.023 4.89 10.25 16.68 1.08 3.18 4.44

FSV-BZ 4.89 6.02 11.10

FSV-CA 0.202 0.578 0.753 4.37 10.33 16.39

FSV-CB 0.133 0.289 0.357 2.80 8.06 9.34

FSV-CH 0.218 0.554 0.740 5.29 10.66 18.13

FSV-CJ 0.192 0.444 0.554 5.42 8.98 13.69

FSV-CK 0.154 0.441 0.577 4.05 10.33 15.66 0.76 2.82 3.91

FSV-CM 4.59 10.04 16.20

FSV-CO 0.175 0.468 0.616 4.18 9.97 15.82

FSV-CP

FSV-CQ 0.144 0.411 0.581 4.19 10.47 16.27

FSV-CT 0.177 0.452 0.750

FSV-CU 0.348 0.649 0.829 3.31 9.74 16.08

FSV-CV 0.187 0.500 0.687 5.83 11.93 17.40 0.96 3.75 4.26

FSV-CX 0.170 0.460 0.630 4.52 10.29 16.54

FSV-CY 0.170 0.490 0.650 4.85 10.60 16.60

FSV-DC 0.196 0.514 0.697 5.10 9.92 14.90 1.29 3.17 4.32

FSV-DE 4.67 10.66 16.90 0.78 2.56 3.62

FSV-DG 0.211 0.487 0.645 5.04 10.88 17.42

FSV-DH 0.171 0.477 0.536 0.025 0.087 0.101 4.79 9.28 13.88 0.80 2.43 3.02

FSV-DL 0.187 0.464 0.595 4.72 9.58 14.61

FSV-DM 0.230 0.660 0.870 3.66 10.82 17.85

FSV-DW

FSV-DY 0.232 0.522 0.675

FSV-EA 0.151 0.401 0.514 3.50 7.30 12.30

FSV-EB 0.182 0.457 0.585 4.69 10.93 18.71

FSV-ED 0.185 0.489 0.664 4.44 10.75 16.55

FSV-EF 0.190 0.510 0.650 5.24 10.47 15.56 0.79 2.70 3.58

FSV-EI 0.290 0.648 0.853 4.29 10.53 16.61

FSV-EJ 0.169 0.504 0.604 4.85 11.27 16.66 0.75 3.08 3.98

FSV-EV 0.186 0.509 0.646 5.50 11.10 17.30

FSV-EY 0.218 0.616 0.805 5.60 12.37 18.84

FSV-FL 0.206 0.498 0.646 4.65 10.50 15.80

FSV-FM 0.122 0.351 0.436 4.40 10.71 15.53

FSV-FS 0.180 0.480 0.680 5.08 10.24 16.81

FSV-FU 0.120 0.064 0.263 3.00 2.00 9.50

n 45 45 45 4 4 5 45 45 45 14 14 14

Min 0.120 0.064 0.263 0.003 0.019 0.023 2.80 2.00 9.34 0.75 2.40 3.02

Mean 0.201 0.491 0.646 0.050 0.088 0.098 4.71 10.18 16.02 0.91 2.93 3.94

Max 0.506 0.690 0.870 0.163 0.212 0.288 7.81 14.49 19.71 1.29 3.75 4.44

SD 0.062 0.100 0.112 0.076 0.088 0.111 0.80 1.75 2.09 0.15 0.36 0.44

CV 12 15 13 47 42 38 10 12 11 11 10 10

NISTa 0.173 0.500 0.631 4.53 10.30 16.22 0.77 2.80 3.95

NISTb 0.183 0.510 0.668 4.29 10.43 15.71 0.88 2.84 3.82

NISTc 0.199 0.500 0.738 3.96 9.18 15.98 0.99 3.26 5.06

Median 0.187 0.495 0.650 0.042 4.72 10.47 16.55 0.89 2.99 4.05

eSD 0.024 0.046 0.070 0.028 0.53 0.43 1.00 0.12 0.28 0.41

eCV 13 9 11 67 11 4 6 13 9 10

G2
Round Robin XXII Laboratory Results

All Lab Report Page 2 / 5

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BG

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BN

FSV-BP

FSV-BS

FSV-BY

FSV-BZ

FSV-CA

FSV-CB

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CQ

FSV-CT

FSV-CU

FSV-CV

FSV-CX

FSV-CY

FSV-DC

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DW

FSV-DY

FSV-EA

FSV-EB

FSV-ED

FSV-EF

FSV-EI

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FM

FSV-FS

FSV-FU

n

Min

Mean

Max

SD

CV

NISTa

NISTb

NISTc

Median

eSD

eCV

Total β-Carotene trans-β-Carotene Total cis-β-Carotene Total α-Carotene Total Lycopene

148 149 150 148 149 150 148 149 150 148 149 150 148 149 150

0.288 0.991 2.580 0.273 0.925 2.420 0.015 0.066 0.160 0.011 0.059 0.119 0.172 0.488 0.421

0.361 0.937 2.606 0.025 0.054 0.118

0.531 0.744 2.220 nd na 0.074 0.172 na 0.329

0.322 1.111 2.931 0.304 1.050 2.749 0.018 0.061 0.182 0.013 0.046 0.104 0.167 0.431 0.371

0.225 0.767 2.024 0.013 0.044 0.092 0.117 0.290 0.272

0.300 0.951 2.381 0.013 0.037 0.074 0.070 0.147 0.132

0.253 0.832 2.129 0.219 0.701 1.799 0.035 0.131 0.330 0.014 0.041 0.096 0.151 0.366 0.299

0.133 0.640 1.673 0.043 0.080 0.097 0.155 0.396 0.437

0.168 0.666 2.193

0.309 0.979 2.557 0.162 0.366 0.319

0.169 0.685 1.784

0.139 0.645 1.627 nd 0.035 0.065 0.101 0.360 0.312

0.245 0.949 2.644 0.217 0.841 2.295 0.028 0.108 0.349 0.019 0.054 0.138 0.141 0.388 0.398

0.294 0.950 2.446

0.202 0.639 1.752 0.004 0.040 0.082 0.130 0.272 0.229

0.224 0.818 2.131

0.234 0.691 1.830

0.195 0.909 2.340 0.185 0.828 2.180 0.010 0.081 0.160 nd 0.052 0.082

0.503 0.948 2.100 0.018 0.066 0.130 0.338 0.655 0.568

0.230 0.840 2.140 0.230 0.840 2.140 nd nd nd 0.030 0.070 0.130

0.330 0.900 2.530

0.282 0.868 2.277

0.278 0.946 2.501

0.479 0.906 2.064 0.216 0.420 0.351

0.188 0.747 1.950 0.009 0.036 0.078 0.073 0.239 0.207

0.180 0.810 2.190

0.200 0.650 1.650 0.014 0.037 0.056 0.112 0.294 0.056

0.239 0.842 2.177

0.249 0.881 2.220

0.215 0.822 2.397

0.309 0.931 2.494

0.362 1.319 2.894

0.284 1.139 3.036 0.082 0.129 0.200 0.203 0.576 1.139

0.260 1.040 2.890

0.205 0.965 2.510

0.350 1.180 3.700

34 34 34 8 8 8 5 5 5 14 16 17 16 15 16

0.133 0.640 1.627 0.185 0.639 1.650 0.010 0.061 0.160 0.004 0.035 0.056 0.070 0.147 0.056

0.275 0.893 2.358 0.229 0.809 2.123 0.021 0.089 0.236 0.022 0.055 0.102 0.155 0.379 0.365

0.531 1.319 3.700 0.304 1.050 2.749 0.035 0.131 0.349 0.082 0.129 0.200 0.338 0.655 1.139

0.094 0.154 0.420 0.040 0.141 0.374 0.010 0.029 0.095 0.020 0.024 0.035 0.064 0.129 0.240

18 12 11 13 13 14 29 23 27 24 18 18 19 20 21

0.231 1.050 2.860 0.201 0.963 2.650 0.030 0.087 0.210 0.026 0.062 0.124

0.203 0.960 2.620 0.010 0.051 0.099 0.124 0.390 0.382

0.193 0.785 2.398 0.011 0.043 0.096 0.062 0.183 0.202

0.257 0.903 2.309 0.218 0.834 2.160 0.018 0.081 0.182 0.014 0.049 0.096 0.153 0.366 0.324

0.077 0.116 0.319 0.025 0.166 0.460 0.011 0.029 0.033 0.007 0.016 0.033 0.044 0.107 0.125

30 13 14 11 20 21 64 36 18 49 33 34 29 29 39

G3 
Round Robin XXII Laboratory Results

All Lab Report Page 3 / 5

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BG

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BN

FSV-BP

FSV-BS

FSV-BY

FSV-BZ

FSV-CA

FSV-CB

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CQ

FSV-CT

FSV-CU

FSV-CV

FSV-CX

FSV-CY

FSV-DC

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DW

FSV-DY

FSV-EA

FSV-EB

FSV-ED

FSV-EF

FSV-EI

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FM

FSV-FS

FSV-FU

n

Min

Mean

Max

SD

CV

NISTa

NISTb

NISTc

Median

eSD

eCV

Total β-Cryptoxanthin Total Lutein Total Zeaxanthin Total Lutein&Zeaxanthin

148 149 150 148 149 150 148 149 150 148 149 150

0.039 0.095 0.063 0.112 0.224 0.179

0.042 0.127 0.075 0.035 0.122 0.089 0.013 0.035 0.029 0.048 0.157 0.118

0.032 0.093 0.066 0.051 0.145 0.126

0.022 0.063 0.047 0.021 0.060 0.043 0.016 0.035 0.021 0.037 0.095 0.064

0.055 0.208 0.193

0.024 0.097 0.074 0.060 0.178 0.130

0.057 0.103 0.088 0.073 0.201 0.185

0.026 0.063 0.041 0.044 0.108 0.087

0.034 0.102 0.093 0.017 0.041 0.016 0.051 0.143 0.109

0.031 0.089 0.061

0.021 0.061 0.037 0.045 0.110 0.083

10 10 10 5 5 5 4 4 4 6 6 6

0.021 0.061 0.037 0.021 0.060 0.043 0.013 0.035 0.016 0.037 0.095 0.064

0.035 0.100 0.074 0.042 0.119 0.098 0.022 0.055 0.038 0.060 0.157 0.121

0.057 0.208 0.193 0.073 0.201 0.185 0.044 0.108 0.087 0.112 0.224 0.179

0.013 0.043 0.045 0.020 0.051 0.052 0.014 0.036 0.033 0.027 0.043 0.037

23 21 23 27 26 28 33 33 38 24 19 21

0.018 0.058 0.047 0.032 0.086 0.079 0.013 0.040 0.037 0.045 0.126 0.116

0.032 0.094 0.065 0.035 0.110 0.089 0.051 0.151 0.122

0.013 0.030 0.021 0.014 0.018 0.009 0.009 0.026 0.016

40 32 33 41 16 10 17 17 13

G4
Round Robin XXII Laboratory Results

All Lab Report Page 4 / 5

Symbol

na 

nd 

italics Value calculated from reported results

n 

Min

Mean Average over all non-NIST reported values.

Max

SD

CV

Median 

eSD 

eCV 

Not available (not reported by participant)

Not detected (not reported or reported as '0', 'not determined', etc.)

Number of non-NIST laboratories reporting quantitative results

Minimum non-NIST reported value.

Table Legend

Interpretation

Maximum non-NIST reported value.

Standard deviation over all non-NIST values.

Coefficient of Variation (% relative standard deviation): 100*SD/Mean

Median over all non-NIST reported values

Robust estimate of SD based on the adjusted median absolute difference from the median (MADe)

Robust estimate of CV, 100*eSD/Median

G5 
Round Robin XXII Laboratory Results

All Lab Report Page 5 / 5

Lab TR aT bC Label Definition

FSV-BA 3±2 -5±3 11±1 Lab Participant code

FSV-BD 8±2 6±3 TR Total Retinol

FSV-BE 3±2 -1±4 aT a-Tocopherol

FSV-BF 9±3 0±3 19±19 bC Total or trans-b-Carotene

FSV-BG 1±3 3±4 29±68 % Bias (Mean ± SD) of individual serum biases

FSV-BH -4±2 -1±2 25±2

FSV-BI -2±2 -2±4 -13±2 Mean Average of (xi

-Mediani)/Mediani

FSV-BJ -1±3 3±5 8±7 SD Standard deviation of (xi

-Mediani)/Mediani

FSV-BK 22±20 1±6 xi Result for analyte in serumi

FSV-BL 15±8 6±6 Mediani Median of non-NIST results in serumi

FSV-BM 1±1 -1±1

FSV-BN 3±6 5±4 -6±4

FSV-BP 74±85 41±23 -35±11

FSV-BS 0±33 -22±15

FSV-BY -3±1 1±3 13±6

FSV-BZ -24±24

FSV-CA 14±5 -3±4

FSV-CB -39±9 -36±11

FSV-CH 14±2 8±5 -27±6

FSV-CJ -7±9 -6±18 -35±10

FSV-CK -13±4 -7±7 5±9

FSV-CM -3±1

FSV-CO -6±1 -7±4 9±5

FSV-CP -25±4

FSV-CQ -17±6 -4±6 -10±3

FSV-CT 0±13 -18±8

FSV-CU 48±33 -13±15 -7±14

FSV-CV 2±3 14±9 31±57

FSV-CX -6±3 -2±2 -8±2

FSV-CY -3±5 1±1 13±15

FSV-DC 5±2 -2±9 2±7

FSV-DE 1±2

FSV-DG 4±8 5±1 7±2

FSV-DH -10±7 -9±9 25±53

FSV-DL -5±4 -7±6 -20±6

FSV-DM 30±6 -4±16 -15±13

FSV-DW -26±4

FSV-DY 11±11 -6±1

FSV-EA -20±1 -27±3 -3±1

FSV-EB -7±4 6±7 -7±10

FSV-ED 0±2 -1±4 11±9

FSV-EF 2±2 2±9

FSV-EI 39±14 -3±6 38±11

FSV-EJ -5±6 4±4 23±11

FSV-EV 1±2 9±7

FSV-EY 22±4 17±3

FSV-FL 3±6 -2±2 14±12

FSV-FM -32±3 -4±5 -1±16

FSV-FS -1±5 2±5 42±16

FSV-FU -61±26 -53±24

NISTa -3±4 -3±1 10±18

NISTb 1±3 -5±4 0±18

NISTc 7±6 -11±6

%Bias Summary

The original analysis listed % Bias for each result for 

each serum calculated relative to the "Trimmed Core 

Lab Average" of that analyte in the serum. The 

summary values reported here are the (arithmetic mean 

± standard deviation) of each laboratory's reported 

results for the analyte estimated relative to each 

serum's median value.

G6 
Appendix H. Shipping Package Inserts for RR23 

Two items were included in each package shipped to an RR23 participant:

• Cover letter. The original letter has been lost. It likely would have described the five

sample materials (lyophilized sera 155, 156, 157, and 159; serum extract 158), given 

guidance on reconstituting the lyophilized samples, stated a date for return of results, to 

whom they should be sent, and who to contact with technical questions. 

• Datasheet. Page H2 reproduces the form.

The cover letter and datasheet were enclosed in a sealed waterproof bag along with the samples.

In addition to the information shipped with the samples, participants received two 

communications (likely via fax or airmail) prior to sample shipment: 

• Workshop notification and instructions on the analysis of the serum extract. Page H3

reproduces this letter. 

• Workshop reminder and request for return of results by September 13, 1991. Page H4

reproduces this letter

H1 
ROUND ROBIN XXIII RESULTS FROM LABORATORY # _________ 

DATE OF ANALYSIS _____________ 

RESULTS IN μg/mL

SAMPLE # ANALYTE RESULT

SERUM 155 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

SERUM 156 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

SERUM 157 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___

SERUM EXTRACT RETINOL _____________

 158 ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

SERUM 159 RETINOL _____________

ALPHA-TOCOPHEROL _____________

VIAL # ___ BETA-CAROTENE (TOTAL) _____________

OPTIONAL ANALYTES : SUPPLY ONE RESULT IF AVAILABLE

SERUM # 155 156 157 159

TRANS-BETA-CAROTENE ______ ______ ______ ______ 

ALPHA-CAROTENE ______ ______ ______ ______ 

RETINYL PALMITATE ______ ______ ______ ______ 

GAMMA-TOCOPHEROL ______ ______ ______ ______ 

LYCOPENE (TOTAL) ______ ______ ______ ______ 

9-CIS-BETA-CAROTENE ______ ______ ______ ______ 

13-CIS-BETA-CAROTENE ______ ______ ______ ______ 

LUTEIN ______ ______ ______ ______ 

ZEAXANTHIN ______ ______ ______ ______ 

BETA-CRYPTOXANTHIN ______ ______ ______ ______ 

 RECONSTITUTE SERUM SAMPLES WITH 1.0 mL of WATER. 

 SERUM EXTRACT 158 IS IN HEXANE.

 NIST FAX # 301-926-8671

H2 
H3 
H4 
Appendix I. Final Report for RR23 

The following 11 pages present the available components of the final report for RR23. 

The report as originally distributed would have had the following components: 

• A “Summary of 1991 Activities”; no version of this component is now available.

• Tables that summarize results for retinol, α-tocopherol, and total β-carotene for

RR21 and RR22. These tables reiterate the results provided in the RR21 and

RR22 Final Reports and so are not included here.

• Tables listing the results and various summary values for retinol, α-tocopherol,

total β-carotene in RR23. These tables are numbered as Table 1 thru Table 3 in

the following pages.

• Tables listing the results and simple summary statistics for trans-β-carotene,

α-carotene, 13-cis-β-carotene, retinyl palmitate, γ-tocopherol, lutein, zeaxanthin,

lutein & zeaxanthin, β-cryptoxanthin, and lycopene. These tables are numbered

as Table 4 to 15 in the following pages.

• Six graphical presentations related to interlaboratory precision for total retinol,

α-tocopherol, and total β-carotene for all round robin studies from March 1986

thru September 1991. The first three of these figures display “Interlaboratory

Precision vs Time”, estimated as a relative standard deviation, for all participants

(with subjective removal of discordant results). The next three display the

relative standard deviation for just the so-called “core labs” (laboratories that

participated in round robin studies prior to RR22).

When the isomeric form of an analyte is not specified, it is likely that most participants 

reported “total” (the sum of all isomers.) Since resolution of γ-tocopherol and 

β-tocopherol is challenging, the results reported as γ-tocopherol can be confidently 

assumed to be γ/β-tocopherol. 

Due to the complex formatting used in the tabular listings, the original laboratory codes 

have been deleted without replacement. However, Appendix J provides a complete 

listing of the RR23 results where the original codes have been altered to ensure 

confidentiality. Appendix J also provides more relevant summary statistics.

I1 
Table 1. A B 

Round Robin XXIII 

Retinal Results Percent Bias from Core Lab Trinmed Average. 

Lab# 

Serl.Ill 

155 

# Ser1.111 

156 

# Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 Extract Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 # Serl.Ill # Ser1.111 Extract 157 159 149 158 Lab # 155 156 157 159 149 158 =======~========== 0.570 0.387 1.099 0.475 ================== 

0.516 0.391 0.997 

0.472 0.255 6.3 -4.4 8.8 -6.1 -3.4 -13.9 0.488 0.488 0.294 0.536 o.3n 0.949 -3.7 -3.5 -1.3 -3.6 0.2 -0.7 

*+0.412 *+0.297 

0.495 0.495 0.0 -6.9 -6.0 -2.2 1.3 *+0.741 *+0.374 0.444 *+0.163 0.509 0.386 -23.1 -26.7 -26.6 -26.1 -9.1 -44.9 1.070 0.523 0.280 0.585 0.411 1.046 -5.0 -4.7 5.9 3.4 -5.4 

0.512 0.373 

0.518 0.578 0.283 9.1 1.5 3.6 2.4 18.3 -4.4 0.963 0.471 0.468 0.342 0.562 0.434 -4.5 -8.0 -4.7 -6.9 -4.2 15.5 

0.539 0.429 

1.108 0.534 0.522 *+0.068 4.9 7.2 9.7 5.5 6.9 -n.o 

0.504 0.403 0.929 

0.989 0.544 0.538 *+0.541 0.6 5.9 -2.1 7.5 10.2 82.8 

0.527 0.397 

0.490 0.500 0.389 -6.0 -0.5 -8.0 -3.2 2.4 31.4 1.013 0.498 0.487 0.358 0.559 0.401 -1.7 -2.0 0.3 -1.6 -0.2 20.9 

0.554 0.431 

1.020 0.523 0.508 *+0.508 4.3 -1.0 1.0 3.4 4.0 71.6 

*+0.726 0.457 *+1.314 

1.073 0.484 0.478 0.273 3.4 6.4 6.2 -4.3 -2.2 -7.8 

0.522 0.386 

*+0.637 0.441 *+0.514 35.4 12.8 30.1 25.9 -9.7 73.6 

0.540 0.370 

1.002 0.469 0.298 -2.6 -4.7 -0.8 -7.3 0.7 0.970 0.500 0.490 0.7 -8.6 -4.0 -1.2 0.3 

0.573 

0.550 

0.425 

0.440 0.980 0.490 0.300 2.6 8.6 -3. 0 -3.2 1.4 

*+0.623 

0.971 0.535 0.522 6.9 4.9 -3.9 5.7 6.9 

+0.447 

0.436 

0.445 

1.080 0.548 0.557 0.344 16.2 7.7 6.9 8.3 14.0 16.2 

0.511 

*+1.227 0.536 o.4n 0.269 -16.6 9.9 21.5 5.9 -2.3 -9.1 0.390 1.006 0.507 0.497 

0.524 -4.7 -3.7 -0.4 0.2 1.8 

*+0.630 *+0.510 

0.398 0.980 0.491 0.481 0.223 -2.2 -1. 7 -3.0 -3.0 -1.5 -24.7 1.048 0.590 0.570 17.5 25.9 3.8 16.6 16.7 0.519 0.386 0.968 0.498 0.616 0.308 0.532 -3.2 -4.7 -4.2 -1.6 26.1 4.1 0.400 o.9n 0.470 0.500 *+0.101 *+0.632 0.411 -0.7 -1.2 -3.3 -7 .1 2.4 -65.9 *+1.183 0.553 0.509 17.9 1.5 17. 1 9.3 4.2 0.508 0.352 *+0.835 0.432 0.649 -5.2 -13.1 -17.3 -14.6 32.9 0.490 0.386 0.914 0.450 0.464 o.5n -8.6 -4.7 -9.5 -11.1 -5.0 

*+0.387 *+0.321 

0.432 1.089 0.558 0.554 0.227 7.6 6.6 7.8 10.3 13.4 -23.3 *+0.634 0.504 *+0.005 -27.8 -20.7 -37.2 3.2 -98.3 0.580 0.410 1.050 0.530 +0.030 8.2 1.2 4.0 4.7 -89.9 0.536 0.394 0.999 0.498 0.1 -2.6 -1 . 1 -1.6 0.474 0.359 0.919 0.455 0.660 0.187 0.521 -11.6 -11.4 -9.0 -10.1 35.1 -36.8 0.391 0.929 0.486 0.498 0.364 -2.8 -3.5 -8.0 -4.0 2.0 23.0 

*0.410 

0.440 0.348 *0.839 0.461 0.188 -17.9 -14.1 -16.9 -8.9 -36.5 0.340 *0.780 *0.410 0.460 *-0.660 -23.5 -16.0 -22.8 -19.0 -5.8 123.0 0.442 0.404 *0.597 *0.367 0.480 *0.146 0.540 -17.5 -0.2 -40.9 -27.5 -1.7 -50.7 0.441 0.981 0.545 0.690 0.346 0.7 8.9 -2.9 7.7 41.3 16.9 

*-0.788 

0.499 0.341 *0.841 0.434 0.530 0.302 -6.9 -15.8 -16.7 -14.2 8.4 2.0 *-0.656 *-1.355 *-0.834 0.064 47.0 62.0 34.2 64.8 -86.9 *0.405 *0.296 *O.n9 *0.360 0.351 *-1.011 -24.4 -26.9 -22.9 -28.9 -28.1 241.6 *-0.658 *-0.493 *1.175 0.559 1.396 0.374 22.8 21.7 16.3 10.5 185.8 26.4 0.448 0.345 *0.723 *-0.206 o.3n 0.286 0.459 -16.4 -14.8 -28.4 -59.3 -22.7 -3.4 0.355 0.983 0.500 0.452 *0.434 -14.4 -12.3 -2.7 -1.2 -7.5 46.6 

TRIMMED ALL LABS (44) ALL LABS 

GRAND AV 0.531 0.401 0.980 0.496 0.510 0.314 AVERAGE 0.521 0.396 1.003 0.504 0.488 0.295 

RSD 

SD 0.080 0.059 0.154 0.088 0.170 0.187 SD 0.041 0.031 0.054 0.037 0.025 0.057 15.0 14.8 15.8 17.8 33.3 59.7 RSD 7.8 7.9 5.4 7.4 5.1 19.2 FRAC REJ 10/44 6/44 14/44 7/43 35.3 

CORE LAB(30) , CORE LAB 

AVERAGE 

SD 

0.538 0.402 1.004 0.506 0.506 0.289 AVERAGE 0.536 0.405 1.010 0.506 0.489 0.296 

RSD 

0.065 

12.2 

0.041 0.131 0.050 0.050 0.137 SD 0.026 0.026 0.055 0.030 0.024 0.047 10.2 13.0 9.9 10.0 47.4 RSD 4.9 6.5 5.4 5.9 5.0 15.8 FRAC REJ 7/30 3/30 6/30 4/29 31.8 

NEW LABSC14) NEW LABS· 

AVERAGE 

SD 

0.514 0.398 0.925 0.475 0.521 0.361 AVERAGE 0.480 0.369 0.892 0.467 0.479 0.266 

RSD 

0.105 0.089 0.192 0.138 0.321 0.260 SD 0.056 0.040 0.153 0.065 0.127 0.125 20.5 22.4 20.8 29.2 61.7 72.0 RSD 11.6 10.8 17.2 14.0 26.4 46.9 FRAC REJ 2/14 2/14 1/14 2/14 16.7 

* = Value removed for ALL LAB TRIMMED AVERAGE. 

+ = Value removed for CORE LAB TRIMMED AVERAGE. 

- = Value removed for NEW LAB TRIMMED AVERAGE. 

I2
able 2. A B 

ound Robin XXIII 

Alpha·Tocopherol Results Percent Bias from Core Lab Trinmed Average. 

Lab # 

Serun 

155 

# Serun 

156 

# Serun # Serun # Serun # Serun Extract Serun # Serun # Serun # Serun # Serun # Serun Extract 157 159 149 158 Lab # 155 156 157 159 149 158 ============-=•=== 7.94 11.06 6.50 ======r-=--======= 

6.87 9.53 5.78 

10.43 10.25 5.57 5.2 8.8 3.4 0.1 ·2.3 ·2.9 10.02 10.23 5.16 7.28 9.30 ·9.0 ·6.2 ·8.0 ·3.8 ·2.4 ·10.1 5.85 10.39 10.34 7.42 *+8.84 ·3.6 ·8.5 ·6.8 ·0.3 ·1.4 5.60 10.23 8.98 *+8.58 *+6.47 *+5.39 ·1.7 ·13.0 ·10.8 ·1.8 ·14.4 49.5 5.88 9.90 5.24 7.71 10.29 6.36 ·14.3 ·47.0 ·6.3 ·5.0 ·8.7 

7.22 9.60 

10.64 10.33 5.11 2.1 1.3 1.3 2.1 ·1.5 ·11.0 6.05 10.29 9.97 6.35 7.90 11.10 ·4.4 ·5.5 ·3.7 ·1.2 ·4.9 10.5 

7.30 10.66 

6.60 *+11.90 10.90 4.70 4.6 9.3 5.1 14.2 3.9 ·18.1 6.32 11.19 10.73 *+9.18 7.60 10.23 ·3.3 4.9 0.6 7.4 2.3 59.9 

7.41 10.10 

6.22 10.82 10.66 4.99 0.7 0.7 ·1.0 3.8 1.6 ·13.1 5.91 10.30 10.60 6.64 7.58 10.06 6.58 ·1.9 ·0.6 ·5.9 ·1.2 1.1 15.7 

7.55 9.89 

10.83 10.88 7.33 0.3 ·1.0 4.8 3.9 3.7 27.6 

7.23 9.89 

6.34 10.64 10.18 6.72 o.o ·2.7 1.0 2.1 ·2.9 17 .1 6.26 10.74 10.38 5.32 +*9.09 10.96 ·4.2 ·2.7 ·0.3 3.1 ·1.0 ·7.3 

7.78 10.22 

7.19 *+12.76 10.33 *+9.92 20.4 7.9 14.5 22.5 ·1.5 72.8 

7.18 *+8.30 

6.26 10.52 5.31 3.0 0.6 ·0.3 1.0 · 7.5 5.76 *+9.41 6.02 7.53 7.30 10.00 ·4.9 ·18.3 ·8.3 ·9.7 ·42.6 31.2 6.60 10.20 10.60 

7.50 ·3.3 ·1.6 5.1 ·2. 1 1. 1 9.90 6.40 10.90 5.60 7.33 9.57 +5.31 ·0.7 ·2.6 1.9 4.6 ·2.4 9.63 10.04 7.60 7.33 ·2.9 ·5.8 ·15.4 ·7.6 ·4.3 32.4 9.70 6.12 9.89 10.86 5.39 *+6.59 *+11.99 ·2.9 ·4.5 ·2.5 ·5.1 3.5 ·6.1 *+8.14 *+12.98 9.28 6.13 7.30 10.70 ·12.7 18.0 29.6 24.5 ·11.5 6.8 +7.20 *+11.60 10.40 ·3.3 5.3 14.6 11.3 ·0.9 

8.08 

7.66 10.25 6.42 10.69 10.69 4.86 1.4 0.9 2.2 2.6 2.0 · 15.3 

8.25 

9.96 6.54 10.50 10.27 7.0 ·2.0 4.1 0.8 ·2. 1 

*+6.50 

10.58 

9.24 

6.80 *+11.58 12.37 5.69 9.3 4.1 8.2 11. 1 17.9 ·0.9 +5.15 9.90 11.93 *+1.52 7.85 10.20 ·13.9 ·9.1 ·18.0 ·5.0 13.7 ·73.5 6.55 10.95 11.10 4.0 0.4 4.3 5.1 5.8 6.96 9.43 6.01 10.08 9.74 *+5.90 ·7.8 ·7.2 ·4.3 ·3.3 ·7 .1 *+8.26 +5.39 *+9.34 9.58 5.31 

8.18 ·21.9 ·18.7 ·14.2 ·10.4 ·8.7 ·7.5 

8.20 

11.06 6.61 *+12. 14 10.66 4.09 8.3 8.9 5.3 16.5 1.6 ·28.7 

7.38 . 10.72 

9.74 

6.84 11.27 *+0.00 8.6 5.5 8.9 7.4 • 100.0 6.08 10.62 3.49 7.99 ·2.3 ·4.1 ·3.2 1.9 ·39.2 10.39 6.44 11.16 5.9 2.2 2.5 7.1 

7.21 

7.84 *·12.24 4.95 *12.59 10.82 4.76 3.9 20.5 ·21.2 20.9 3.2 ·17.0 

*·5.70 

9.43 5.84 10.40 10.50 3.97 ·4.5 ·7.2 ·7.0 ·0.2 0.1 ·30.8 *·7.00 *4.60 10.80 3.23 6.85 9.44 ·24.5 ·31.1 ·26.8 3.6 ·43.7 6.29 *9.48 10.29 *·12.02 7.80 ·9.3 ·7.1 0.2 ·9.0 ·1.9 109.4 

*·12.23 *·15.38 

9.67 6.61 9.74 10.24 3.06 3.3 ·4.8 5.3 ·6.6 ·2.4 ·46.7 *·10.24 *15.04 14.49 62.0 51.4 63.1 44.3 38.1 

*6.28 

7.86 

*8.50 

10.48 6.07 10.73 11.05 3.70 4.1 3.2 ·3.4 3.0 5.4 ·35.6 5.20 *8.96 0.20 ·16.9 ·16.4 ·17 .1 ·14.0 ·98.1 7.23 9.71 6.09 *6.39 10.71 *·19.62 8.18 *11.29 ·4.3 ·4.4 ·3.0 ·38.7 2.1 241.8 7.14 *4.73 24.38 6.02 8.3 11.1 13.7 ·54.6 132.4 4.9 

TRIMMED ALL LABS C44) ALL LABS 

SD 

GRAND AV 7.52 

0.97 

10.01 6.30 10.51 10.52 5.99 AVERAGE 7.56 10.09 6.20 10.45 10.49 5.32 

RSD 12.9 

1.44 0.88 1.58 2.91 3.27 SD 0.38 0.54 0.53 0.42 0.35 1.22 14.4 14.0 15.0 27.6 54.5 RSD 5.1 5.3 8.6 4.0 3.4 22.9 FRAC REJ 8/44 10/44 3/44 14/43 20.0 

CORE LABC32) CORE LABS 

AVERAGE 

SD 

7.44 

0.62 

9.89 6.30 10.69 10.35 5.79 AVERAGE 7.55 10.16 6.28 10.42 10.50 5.74 

RSD 8.3 

1.16 

11.8 

0.60 0.90 1.017 2.10 SD 0.37 0.55 0.34 0.40 0.32 0.96 9.6 8.4 9.8 36.3 RSD 4.9 5.4 5.4 3.9 3.0 16.7 FRAC REJ 5/32 5/32 6/32 8/31 19.2 

NEW LABSC12) NEW LABS 

AVERAGE 

SD 

7.71 

1.60 

10.27 

2.07 

6.30 10.05 11.07 6.65 AVERAGE 7.46 9.85 5.94 10.05 10.60 4.03 

RSD 20.8 

1.43 2.65 5.94 5.61 SD 0.59 0.79 0.75 2.65 0.32 1.04 20.2 22.8 26.4 53.7 84.3 RSD 7.9 8.0 12.7 26.4 3.0 25.8 FRAC REJ 2/12 3/12 1/12 0/12 22.2 

* = Value removed for ALL LAB TRIMMED AVERAGE. 

+ = Value removed for CORE LAB TRIMMED AVERAGE. 

· = Value removed for NEW LAB TRIMMED AVERAGE. 

I3
able 3. A B 

OU"td Robin XXII I 

Total Beta-Carotene Results Percent Bias from Core Lab Trinmed Average. 

Lab # 

Serun 

155 

# Serun # Serun # Serun # Serun # Serun # Serun # serun # Serun # Serun # 156 157 159 149 Lab # 155 156 157 159 149 ================== 0.442 0.980 0.093 0.891 ================== 0.979 0.333 0.750 19.1 19.2 2.2 1.4 14.9 0.083 0.827 0.767 

0.283 -10.2 ·8.8 -8.8 -5 .9 -10.1 0.851 0.106 0.757 0.744 0.293 0.593 -23.7 3.5 16.5 -13.9 ·12.7 0.065 0.689 0.645 

0.342 ·21.0 ·27.9 ·28.6 ·21.6 ·24.3 0.656 *+0.170 0.893 

0.389 -7.8 ·20.2 86.8 1.6 

0.437 

0.853 0.109 0.935 0.950 4.7 3.7 19.8 6.4 11.4 1.046 0.090 *+1.200 0.937 

0.348 17.8 27.3 ·1.1 36.5 10.0 0.891 0.080 0.782 0.946 

0.396 ·6.2 8.4 • 12. 1 ·11.0 11.0 

0.411 

0.818 0.092 0.902 0.991 6.7 ·0.5 1.1 2.6 16.3 0.921 0.112 1.050 1.111 

*0.080 10.8 12.0 23.1 19.5 30.3 *0.455 *0.044 *0.403 0.949 

0.440 ·78.4 ·44.7 ·51.4 -54.1 11.4 

0.379 

0.820 0.090 0.950 0.900 18.6 ·0.2 ·1.1 8.1 5.6 

0.344 

0.852 0.094 0.930 0.842 2.2 3.6 3.3 5.8 ·1.2 0.965 0.118 1.065 0.906 

0.362 ·7.3 17.4 29.7 21.2 6.3 0.933 0.098 0.908 0.951 0.390 ·2.4 13.5 7.7 3.3 11.6 

0.303 

0.891 0.080 0.872 0.948 5.1 8.4 ·12.1 ·0.8 11.2 0.697 0.067 +0.602 

+0.234 ·18.4 ·15.2 ·26.4 ·31.5 0.658 0.057 0.688 0.747 

0.408 ·36.9 ·20.0 ·37.4 ·21.7 ·12.4 0.851 0.112 0.923 0.685 9.9 3.5 23.1 5.0 ·19.7 0.597 +0.024 1.139 

0.420 ·27.4 ·73.6 33.6 0.823 0.124 0.982 13.2 0. 1 36.3 11. 7 0.418 0.893 0.091 0.998 12.5 8.7 0.3 13.5 

0.423 

0.433 0.873 0.099 0.826 0.810 16.7 6.2 8.2 -6.0 -5.0 0.842 0.113 0.950 1.040 14.0 2.4 24.2 8.1 22.0 0.258 *0.542 0.054 0.660 -30.5 ·34.1 ·40.7 ·24.9 *·1.320 *·1.750 *·1.640 *·1.910 0.840 255.8 112.9 1702.2 117.3 ·1.4 0.381 1.055 0.092 *·1.836 1.180 2.7 28.3 1. 1 108.9 38.4 0.482 1.049 *·0.214 0.962 0.640 29.9 27.6 135.2 9.4 ·24.9 0.239 *0.397 0.052 0.593 0.965 

0.308 ·35.6 ·51.7 ·43.1 ·32.5 13.2 0.881 0.069 0.904 1.319 ·17.0 7.2 ·24.2 2.8 54.8 *·0.074 *0.382 *0.020 *·0.246 0.666 -80.1 -53.5 ·78.0 ·72.0 ·21.9 0.353 0.766 0.089 0.718 0.691 -4.9 ·6.8 ·2.2 ·18.3 ·18.9 

TRIMMED ALL LABS (33) ALL LABS 

========= GRAND AV 0.378 0.823 0.139 ========= 

SD 

0.898 0.894 AVERAGE 0.366 0.845 0.090 0.856 0.852 

RSD 

0.199 0.246 0.276 0.324 0.154 SD 0.066 0.126 0.020 0.132 0.114 52.7 29.9 199.2 36.0 17.2 RSD 18.0 15.0 22.2 15.4 13.4 FRAC REJ 3/31 5/32 6/32 5/31 

CORE LAB (23) CORE LABS 

AVERAGE 0.348 0.804 0.089 0.856 0.891 AVERAGE 0.371 0.822 0.091 0.879 0.890 SD 

RSD 

0.086 0.152 0.031 0.179 0.121 SD 0.050 0.131 0.018 0.109 0.078 24.8 18.9 34.3 20.9 13.6 RSD 13.6 15.9 19.3 12.4 8.8 FRAC REJ 2/19 1/20 3/20 3/19 

NEW LABSC10) NEW LABS 

AVERAGE 0.426 0.854 0.221 0.965 0.898 AVERAGE 0.371 o.m 0.080 0.844 0.898 SD 

RSD 

0.303 0.36 0.449 0.475 0.226 so 0.080 0.234 0.031 0.152 0.226 71.2 42.1 203.0 49.2 25.2 RSD 21.6 30.2 39.2 18.0 25.2 FRAC REJ 2/12 1/12 2/12 3/12 

* = Value removed for ALL LAB TRIMMED AVERAGE. 

+ = Value removed for CORE LAB TRIMMED AVERAGE. - = Value removed for NEW LAB TRIMMED AVERAGE. 

I4
ROUND ROBIN XXIII 

"able 4. Table 5. 

irans Beta-Carotene Alpha-Carotene 

Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # 

Lab # 155 156 157 159 149 Lab # 155 156 157 159 149 

z======z========== =======s========= 

0.360 0.780 0.086 0.826 0.925 0.019 0.043 0.024 0.045 

0.388 0.877 0.112 0.984 1.050 0.016 0.026 0.020 0.042 0.044 

0.080 0.396 0.039 0.353 0.841 0.018 0.023 0.017 0.033 0.035 

0.388 0.755 0.112 0.899 0.828 0.015 0.040 0.014 0.069 0.054 

0.290 0.710 0.010 0.740 0.840 0.031 0.041 0.018 0.055 0.059 

0.241 0.634 0.084 0.650 0.018 0.034 0.020 0.052 0.046 

0.359 0.575 0.069 0.610 0.710 0.018 0.027 0.018 0.027 0.538 

0.019 0.026 0.018 0.056 

0.019 0.027 0.019 0.038 0.037 ======== 0.046 0.054 0.066 

AVERAGE 0.301 0.675 0.073 0.735 0.843 0.013 0.024 0.014 0.035 0.036 

SD 0.112 0.158 0.038 0.228 0.125 0.004 0.015 0.003 0.129 

RSD 37.1 23.4 51.4 31.0 14.8 0.065 0.086 0.079 0.109 0.080 

0.009 0.025 0.018 0.030 0.041 

Table 6. 

AVG 0.020 0.034 0.022 0.050 

9+13·CIS·Beta·Carotene SD 0.015 0.017 0.018 0.022 

RSD 72.7 50.4 82.5 43.7 

Serun # Serun # Serun # Serun # 

Lab # 155 156 157 159 

56 0. 036 0.38 0.006 0.073 

Table 7. Table 8. 

Retinyl Palmitate Gamia Tocopherol 

Serun # Serun # Serun # Serun # Serun # Serun # Serun # serun # Serun # Serun # 

Lab # 155 156 157 159 149 Lab # 155 156 157 159 149 

===========--====== ===============--== 

0.015 0.008 0.01 0.019 0.019 2.64 2.23 1.79 2.88 3.18 

0.033 0.037 0.047 0.041 0.032 2.09 1.86 1.53 2.66 2.89 

0.227 0.237 0.425 0.249 0.212 2.22 2.40 1.78 3.45 3.17 

2.14 1.80 1.51 2.59 2.56 

2.07 1.85 1.64 2.64 2.72 

2.65 2.28 1.99 3.40 3.12 

2.90 1.72 1.89 3.32 2.82 

2.24 1.83 1.76 2.74 

2.15 1.95 1.62 2.70 2.70 

========= 2.43 2.05 1.81 2.94 3.08 

AVG 0.092 0.094 0.161 0.103 0.088 2.01 1.91 1.32 2.87 3.75 

SD 0.118 0.125 0.230 0.127 0.088 2.49 1.85 1.45 3.08 

RSD 128.2 132.6 142.9 123.2 100.7 

===== 

AVG 2.34 1.98 1.67 2.93 3.00 

SD 0.28 0.22 0.20 0.32 0.40 

RSD 12.1 10.9 11.7 10.9 13.4 

Table 9. Table 10. 

Lute in Zeaxanthin 

Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # Serun # 

Lab # 155 156 157 159 149 Lab # 155 156 157 159 149 

================== 

0.087 0.110 0.071 0.109 0.122 0.026 0.036 0.024 0.038 0.035 

0.223 0.248 0.183 0.270 0.201 0.023 0.020 0.012 0.020 0.035 

0.138 0.179 0.126 0.257 0.048 0.064 0.027 0.061 0.041 

0.050 0.063 0.038 0.055 0.060 

0.091 0.116 0.063 0.114 0.102 

========= ===== AVG 0.118 0.143 0.096 0.161 0.114 AVG 0.032 0.040 0.021 0.040 0.038 

SD 0.067 0.072 0.058 0.096 0.054 SD 0.014 0.022 0.008 0.021 0.003 

RSD 56.5 50.0 60.5 59.9 47.0 RSD 42.2 55 .7 37.8 51.8 8.6 I5
ROUND ROBIN XXIII 

Table 11. Table 12. 

Zeaxanthin & Lutein Beta·Cryptoxanthin 

Serl.Ill # Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 # Ser1.111 # Serl.Ill # 

Lab # 155 156 157 159 149 Lab# 155 156 157 159 149 

================== =======----======= 

0.100 0.139 0.083 0.136 0.145 0.061 0.069 0.052 0.099 0.093 

0.113 0.144 0.074 0.144 0.178 0.081 0.064 0.069 0.096 0.097 

0.166 0.209 0.126 0.200 0.224 0.066 0.063 0.057 0.083 0.095 

0.185 0.239 0.171 0.317 0.102 0.098 0.107 0.139 0.127 

0.084 0.109 0.069 0.109 0.051 0.054 0.067 0.082 0.103 

0.081 0.092 0.060 0.081 0.049 0.043 0.037 0.067 

0.051 0.048 0.050 0.082 0.089 

0.297 0.283 0.320 0.319 0.208 

0.033 0.032 0.015 0.044 0.063 

=•====== =======z 

AVG 0.122 0.155 0.097 0.164 0.182 AVG 0.088 0.084 0.086 0.112 

SD 0.044 0.057 0.043 0.085 0.040 SD 0.081 0.077 0.091 0.082 

RSD 36.0 36.9 44.2 51.5 21.8 RSD 92.1 91.9 106.1 72.6 

Table 13. 

Lycopene 

Ser1.111 # Serl.Ill # Ser1.111 # Serl.Ill # Serl.Ill # 

Lab # 155 156 157 159 149 :E:::::::=:::::::: 

0.583 0.423 0.827 0.352 0.660 

0.507 0.372 0.706 0.361 0.290 

0.339 0.419 0.844 0.312 

0.602 0.416 0.773 0.426 0.360 

0.507 0.411 0.745 0.434 

0.571 0.422 0.811 0.800 0.488 

0.674 0.512 1.039 0.497 0.431 

0.107 0.181 0.378 0.143 0.388 

0.776 0.585 1.097 0.573 

0.780 0.722 1.520 0.727 0.420 

0.206 0.172 0.305 0.156 0.147 

0.779 0.653 0.941 0.590 0.655 

0.470 0.327 0.633 0.276 

0.257 0.228 0.385 0.227 0.239 

0.455 0.303 0.743 0.576 

0.577 0.481 0.748 0.425 0.392 

0.506 0.322 0.652 0.302 0.366 

========= 

AVG 0.512 0.409 0.773 0.413 0.400 

SD 0.196 0.153 0.289 0.189 0. 158 

RSD 38.4 37.3 37.4 45.8 39.6 

I6
lnterlaboratory Precision vs Time 

Retinal 

Percent 

30 

+ + + 

20 

+ 

+ 

+ + 

10 

+ + 

+ + 

+ + + + + 

3/86 10/86 4/87 7187 1/88 7/88 9/88 1/89 4/89 8/89 1/90 6/90 9/90 1/91 6/91 8/91 

Data Controls Available 

+ % SD Retfnof 

I7
lnterlaboratory Precision vs Time 

Alpha-Tocopherol 

Percent 

30 

* 

201* * * 

* 

10 

* 

* * * * * * * * * * 

0 J 

3/86 10/86 4/87 7187 1/88 7/88 9/88 1/89 4/89 8189 1/90 5/90 9/90 1/91 5/91 8/91 

Date 

Controls Available * % SD Tocopherol 

I8
lnterlaboratory Precision vs Time 

Beta-Carotene 

Percent 

30 

z 

z z 

20 z 

z z 

z z z z 

z 

10 z z z z z 

3/88 10/86 4/87 7187 1/88 7188 9188 1/89 4/89 8189 1/90 5/90 9/90 1/91 5/91 8/91 

Data 

Controls Available 

Z 'l SD Beta-Carotene 

I9
RSD 

Average Relative Standard Deviation 

Core Labs 

Retinal 

2Qr ----------------·-·----------·------------··----·------------··-·---·-·-·------···----·--···---·---··----------------···--··-------- -· ·-····· · ---··-----I 

15 10 

5 

0 

RR# 7 

3/86 

8 9 11 

10/86 4187 7187 

12 13 14 

1/88 17/88 9/88 

Free Controls 

15 

1/89 

16 17 18 

4/89 8/891 1/90 

19 20 

5/90 9/90 

21 

1/91 

22 23 

5/91 8/91 

SRM Available 

I10
RSD 

Average Relative Standard Deviation 

Core Labs 

Alpha-Tocopherol 

mm •mm••••••••••••• ••••••••mm•mmm•m 

15 10 

5 

0 

RR# 7 8 9 11 

3/86 10/86 4/87 7/87 

12 13 

1/88 17 /88 

14 

9/88 

15 

1/89 

16 17 18 

4/89 8/891 1/90 

19 20 

5/90 9/90 

Free Controls SRM Available 

21 22 23 

1/91 5/91 8/91 

I11
Average Relative Standard Deviation 

Core Labs 

Total Beta-Carotene 

RSD 

20 

15 . ····-----· .. ··---·····-·-·------- ....... ---···-··-··---·· .... ·-·--·-····· . ···········-·····- ······-·--···· 10 

5 

0 I t;·.•.·;-.·t--.·-·.·.·.J 1.·.·. 

RR# 7 8 9 11 

3/86 10/86 4/87 7187 

12 13 

1/88 17188 

14 

9/88 

15 

1/89 

16 17 18 

4/89 8/891 1/90 

19 20 

5/90 9/90 

Free Controls SRM Available 

21 22 23 

1/91 5/91 8/91 

I12
Appendix J. Updated “All-Lab Report” for RR23 

The following five pages are an updated version of an “All-Lab” report for RR23. This 

report has three parts: 

• pages 1 thru 4 list results for all analytes reported by at least twice, counting both

participants and NIST analysts.

• page 5 lists values for all analytes reported by only once. This page also provides

a legend for pages 1 thru 4.

• page 6 summarizes each participant’s performance for total retinol, α-tocopherol, 

and total β-carotene. These summaries are compatible with the percent bias 

evaluation advice given in the RR23 Report. However, the current bias 

summaries are estimated relative to the median of all reported values for each 

analyte in each serum rather than to the “Trimmed Core Lab Average” used in the 

original and detailed in Appendix J. These original reference values were 

estimated from on-time results of the more experienced participants, with 

subjective exclusion of results deemed non-representative.

To ensure confidentiality, the laboratory identifiers used in this “All-Lab Report” have 

been altered from those used in RR23. The only attributed results are those reported by 

NIST. The NIST results are not used in the assessment of the consensus summary results 

of the study. 

J1 
Round Robin XXIII Laboratory Results

All Lab Report Page 1 / 6

Total Retinol Retinyl Palmitate α-Tocopherol γ/β-Tocopherol

Lab 155 156 157 158 159 155 156 157 158 159 155 156 157 158 159 155 156 157 158 159

FSV-BA 0.559 0.401 1.020 0.508 0.523 0.033 0.037 0.047 0.041 7.55 9.89 6.34 6.72 10.64 2.07 1.85 1.64 2.64

FSV-BD 0.562 0.434 1.108 0.068 0.534 7.90 11.10 6.60 4.70 11.90

FSV-BE 0.504 0.403 0.929 0.389 0.490 7.41 10.10 5.91 6.64 10.30

FSV-BF 0.539 0.429 0.989 0.451 0.544 7.30 10.66 6.32 9.18 11.19 2.22 2.40 1.78 3.45

FSV-BG 0.536 0.377 0.949 0.495 7.28 9.30 5.85 10.39

FSV-BH 0.554 0.431 1.073 0.273 0.484 nd nd nd nd 7.23 9.89 6.26 5.32 10.74 2.65 2.28 1.99 3.40

FSV-BI 0.516 0.391 0.997 0.294 0.488 nd nd nd nd 6.87 9.53 5.78 5.16 10.02 2.09 1.86 1.53 2.66

FSV-BJ 0.524 0.398 0.980 0.223 0.491 0.227 0.237 0.425 0.249 7.66 10.25 6.42 4.86 10.69 2.43 2.05 1.81 2.94

FSV-BK 0.630 0.510 1.048 0.590 8.08 9.96 6.54 10.50

FSV-BL 0.623 0.436 1.080 0.344 0.548 7.33 9.70 6.12 5.39 9.89

FSV-BM 0.511 0.390 1.006 0.507 7.30 10.70 7.20 11.60

FSV-BN 0.499 0.341 0.841 0.302 0.434 nd nd nd nd 7.86 10.48 6.07 3.70 10.73

FSV-BP 0.540 0.441 0.981 0.346 0.545 12.23 15.38 10.24 na 15.04

FSV-BS 0.448 0.345 0.723 0.286 0.206

FSV-BX 0.522 0.386 1.002 0.298 0.469 7.78 10.22 6.26 5.31 10.52 2.24 1.83 1.76 2.74

FSV-BY 0.570 0.387 1.099 0.255 0.475 0.015 0.008 0.010 0.019 7.94 11.06 6.50 5.57 10.43 2.64 2.23 1.79 2.88

FSV-BZ 7.18 8.30 5.76 7.53 9.41

FSV-CA 0.585 0.411 1.046 0.283 0.518 7.71 10.29 6.36 5.11 10.64

FSV-CH 0.577 0.432 1.089 0.227 0.558 8.18 11.06 6.61 4.09 12.14

FSV-CJ 0.412 0.297 0.741 0.163 0.374 7.42 8.84 5.60 8.58 10.23

FSV-CK 0.726 0.457 1.314 0.514 0.637 9.09 10.96 7.19 9.92 12.76 2.90 1.72 1.89 3.32

FSV-CM 7.33 9.57 5.31 7.60 9.63

FSV-CO 0.512 0.373 0.963 0.345 0.471 7.22 9.60 6.05 6.35 10.29

FSV-CP

FSV-CT 0.459 0.355 0.983 0.434 0.500

FSV-CU 0.508 0.352 0.835 0.432 6.96 9.43 6.01 10.08

FSV-CV 0.532 0.400 0.977 0.101 0.470 6.50 9.24 5.15 1.52 9.90 2.01 1.91 1.32 2.87

FSV-CX 0.410 0.340 0.780 0.660 0.410 6.85 9.44 6.29 12.02 9.48

FSV-CY 0.540 0.370 0.970 0.500 7.30 10.00 6.60 10.20

FSV-DE 7.60 10.23 6.22 4.99 10.82 2.14 1.80 1.51 2.02 2.59

FSV-DG 0.527 0.397 1.013 0.358 0.498 7.58 10.06 6.58 7.33 10.83

FSV-DH 0.447 0.445 1.227 0.269 0.536 6.59 11.99 8.14 6.13 12.98

FSV-DL 0.490 0.386 0.914 nd 0.450 5.90 8.26 5.39 5.31 9.34

FSV-DM 0.474 0.359 0.919 0.187 0.455 7.84 12.24 4.95 4.76 12.59

FSV-DO 0.509 0.386 1.070 0.280 0.523 6.47 5.39 5.88 5.24 9.90

FSV-DW

FSV-DY 0.573 0.425 0.971 0.535

FSV-EA 0.440 0.348 0.839 0.188 0.461 5.70 7.00 4.60 3.23 10.80

FSV-EB 0.506 0.378 1.057 0.308 0.496 7.44 10.13 6.33 3.29 10.76

FSV-EF 0.550 0.440 0.980 0.300 0.490 7.50 9.90 6.40 5.60 10.90 2.15 1.95 1.62 2.70

FSV-EI 0.658 0.493 1.175 0.374 0.559 8.18 11.29 7.14 6.02 4.73 2.38 1.79 1.61 1.15

FSV-EJ 0.387 0.321 0.634 0.005 ns 8.20 10.72 6.84 0.00 ns 2.49 1.85 1.45

FSV-EV 0.632 0.411 1.183 ns 0.553 7.85 10.20 6.55 ns 10.95

FSV-EY 0.519 0.386 0.968 0.308 0.498 8.25 10.58 6.80 5.69 11.58

FSV-FL 0.521 0.391 0.929 0.364 0.486 7.21 9.43 5.84 3.97 10.40

FSV-FM 0.405 0.296 0.779 1.011 0.360 7.23 9.71 6.09 19.62 6.39

FSV-FR 0.536 0.394 0.999 0.498 7.99 10.39 6.44 11.16

FSV-FS 0.442 0.404 0.597 0.146 0.367 7.80 9.67 6.61 3.04 9.74

FSV-FU 0.788 0.656 1.355 0.834 6.28 8.50 5.20 8.96

FSV-GB 0.580 0.410 1.050 0.030 0.530 7.38 9.74 6.08 3.49 10.62

n 45 45 45 35 44 3 3 3 0 3 45 45 45 36 44 13 13 13 1 12

Min 0.387 0.296 0.597 0.005 0.206 0.015 0.008 0.010 0.019 5.70 5.39 4.60 0.00 4.73 2.01 1.72 1.32 1.15

Mean 0.531 0.400 0.982 0.311 0.496 0.092 0.094 0.161 0.103 7.52 10.01 6.30 5.92 10.52 2.34 1.96 1.67 2.02 2.78

Max 0.788 0.656 1.355 1.011 0.834 0.227 0.237 0.425 0.249 12.23 15.38 10.24 19.62 15.04 2.90 2.40 1.99 3.45

SD 0.079 0.059 0.153 0.182 0.087 0.118 0.125 0.230 0.127 0.96 1.42 0.87 3.25 1.56 0.27 0.21 0.19 0.60

CV 10 9 11 18 10 52 53 54 51 8 9 9 17 10 9 9 9 17

NISTc 0.566 0.411 0.990 0.516 7.63 9.81 6.38 10.39 2.12 1.88 1.57 2.66

Median 0.524 0.394 0.983 0.298 0.497 7.42 10.00 6.29 5.32 10.57 2.24 1.86 1.64 2.81

eSD 0.052 0.046 0.099 0.105 0.051 0.56 0.83 0.46 1.89 0.77 0.25 0.10 0.21 0.23

eCV 10 12 10 35 10 8 8 7 36 7 11 6 13 8

J2
Round Robin XXIII Laboratory Results

All Lab Report Page 2 / 6

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BG

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BN

FSV-BP

FSV-BS

FSV-BX

FSV-BY

FSV-BZ

FSV-CA

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CT

FSV-CU

FSV-CV

FSV-CX

FSV-CY

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DO

FSV-DW

FSV-DY

FSV-EA

FSV-EB

FSV-EF

FSV-EI

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FM

FSV-FR

FSV-FS

FSV-FU

FSV-GB

n

Min

Mean

Max

SD

CV

NISTc

Median

eSD

eCV

Total β-Carotene trans-β-Carotene Total cis-β-Carotene

155 156 157 158 159 155 156 157 158 159 155 156 157 158 159

0.396 0.818 0.092 0.902 0.360 0.780 0.086 0.826 0.036 0.380 0.006 0.073

0.437 1.046 0.090 1.200

0.283 0.851 0.106 0.757

0.411 0.921 0.112 1.050 0.388 0.877 0.112 0.984 0.023 0.044 nd 0.066

0.333 0.750 0.083 0.827

0.362 0.933 0.098 0.908

0.359 0.575 0.069 0.610

0.482 1.049 0.214 0.962

0.074 0.382 0.020 0.246

0.442 0.980 0.093 0.891

0.408 0.851 0.112 0.923

0.293 0.593 0.065 0.689

0.080 0.455 0.044 0.403 0.080 0.396 0.039 0.353 nd 0.059 0.005 0.050

0.389 0.853 0.109 0.935

0.303 0.697 0.067 0.602

0.353 0.766 0.089 0.718

0.420 0.823 0.124 0.982 0.388 0.755 0.112 0.899 0.032 0.068 0.012 0.083

0.390 0.891 0.080 0.872

0.290 0.710 0.010 0.740

0.440 0.820 0.090 0.950

0.348 0.891 0.080 0.782

0.344 0.965 0.118 1.065

0.234 0.658 0.057 0.688

0.433 0.873 0.099 0.826

0.342 0.656 0.170 0.893

0.241 0.634 0.084 0.019 ns

0.379 0.852 0.094 0.930

0.258 0.542 0.054 0.660

0.376 0.829 0.061 0.865

0.308 0.881 0.069 0.904

na 0.597 0.024 ns

0.423 0.842 0.113 0.950

0.239 0.397 0.052 0.593

0.418 0.893 0.091 0.998

0.381 1.055 0.092 nd 1.836

0.420 0.823 0.124 0.982

32 33 33 0 32 7 7 7 1 6 3 4 3 0 4

0.074 0.382 0.020 0.246 0.080 0.396 0.010 0.353 0.023 0.044 0.005 0.050

0.350 0.795 0.090 0.868 0.301 0.675 0.073 0.019 0.735 0.030 0.138 0.008 0.068

0.482 1.055 0.214 1.836 0.388 0.877 0.112 0.984 0.036 0.380 0.012 0.083

0.095 0.176 0.038 0.261 0.112 0.158 0.038 0.228 0.007 0.162 0.004 0.014

20 17 18 14 29 18 34 23 18 43 31 17

0.419 0.853 0.089 0.786

0.378 0.842 0.091 0.898 0.359 0.710 0.084 0.783

0.064 0.117 0.031 0.125 0.043 0.113 0.042 0.214

17 14 34 14 12 16 49 27

J3
Round Robin XXIII Laboratory Results

All Lab Report Page 3 / 6

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BG

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BN

FSV-BP

FSV-BS

FSV-BX

FSV-BY

FSV-BZ

FSV-CA

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CT

FSV-CU

FSV-CV

FSV-CX

FSV-CY

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DO

FSV-DW

FSV-DY

FSV-EA

FSV-EB

FSV-EF

FSV-EI

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FM

FSV-FR

FSV-FS

FSV-FU

FSV-GB

n

Min

Mean

Max

SD

CV

NISTc

Median

eSD

eCV

Total α-Carotene Total Lycopene trans-Lycopene Total β-Cryptoxanthin

155 156 157 158 159 155 156 157 158 159 155 156 157 158 159 155 156 157 158 159

0.031 0.041 0.018 0.055 0.571 0.422 0.811 0.800 0.066 0.063 0.057 0.083

0.015 0.040 0.014 0.069 0.507 0.411 0.745 0.434

0.339 0.419 0.844 0.312

0.018 0.034 0.020 0.052 0.674 0.512 1.039 0.497 0.102 0.098 0.107 0.139

0.016 0.026 0.020 0.042 0.507 0.372 0.706 0.361 0.061 0.069 0.052 0.099

0.019 0.027 0.019 0.038 0.206 0.172 0.305 0.156

0.009 0.025 0.018 0.030 0.506 0.322 0.652 0.302 0.033 0.032 0.015 0.044

0.065 0.086 0.079 0.109 0.577 0.481 0.748 0.425 0.297 0.283 0.320 0.319

0.019 0.043 0.024 0.045 0.583 0.423 0.827 0.352

0.018 0.023 0.017 0.033 0.602 0.416 0.773 0.426 0.081 0.064 0.069 0.096

0.018 0.027 0.018 0.027 0.107 0.181 0.378 0.143 0.051 0.054 0.067 0.082

0.019 0.030 0.019 0.036 0.470 0.327 0.633 0.276 0.049 0.043 0.037 0.067

0.046 0.054 0.779 0.653 0.941 0.590

0.780 0.722 1.520 0.727

0.013 0.024 0.014 0.035 0.257 0.228 0.385 0.227 0.051 0.048 0.050 0.082

0.019 0.026 0.018 0.056 0.776 0.585 1.097 0.573

0.004 0.015 0.003 ns 0.455 0.303 0.743 ns

14 15 14 0 14 16 16 16 0 15 1 1 1 0 1 9 9 9 0 9

0.004 0.015 0.003 0.027 0.107 0.172 0.305 0.143 0.033 0.032 0.015 0.044

0.020 0.034 0.021 0.049 0.512 0.414 0.781 0.420 0.506 0.322 0.652 0.302 0.088 0.084 0.086 0.112

0.065 0.086 0.079 0.109 0.780 0.722 1.520 0.800 0.297 0.283 0.320 0.319

0.014 0.017 0.017 0.021 0.203 0.156 0.297 0.193 0.081 0.077 0.091 0.082

22 20 22 19 26 22 20 24 27 27 29 26

0.041 0.043 0.035 0.055 0.663 0.536 0.915 0.492 0.663 0.536 0.915 0.492 0.053 0.053 0.039 0.067

0.018 0.027 0.018 0.044 0.539 0.418 0.761 0.425 0.061 0.063 0.057 0.083

0.002 0.006 0.002 0.016 0.162 0.137 0.156 0.219 0.018 0.022 0.018 0.024

12 22 12 36 30 33 21 52 29 35 31 28

J4
Round Robin XXIII Laboratory Results

All Lab Report Page 4 / 6

Lab

FSV-BA

FSV-BD

FSV-BE

FSV-BF

FSV-BG

FSV-BH

FSV-BI

FSV-BJ

FSV-BK

FSV-BL

FSV-BM

FSV-BN

FSV-BP

FSV-BS

FSV-BX

FSV-BY

FSV-BZ

FSV-CA

FSV-CH

FSV-CJ

FSV-CK

FSV-CM

FSV-CO

FSV-CP

FSV-CT

FSV-CU

FSV-CV

FSV-CX

FSV-CY

FSV-DE

FSV-DG

FSV-DH

FSV-DL

FSV-DM

FSV-DO

FSV-DW

FSV-DY

FSV-EA

FSV-EB

FSV-EF

FSV-EI

FSV-EJ

FSV-EV

FSV-EY

FSV-FL

FSV-FM

FSV-FR

FSV-FS

FSV-FU

FSV-GB

n

Min

Mean

Max

SD

CV

NISTc

Median

eSD

eCV

Total Lutein Total Zeaxanthin Total Lutein&Zeaxanthin

155 156 157 158 159 155 156 157 158 159 155 156 157 158 159

0.166 0.209 0.126 0.200

0.087 0.110 0.071 0.109 0.026 0.036 0.024 0.038 0.113 0.146 0.095 0.147

0.100 0.139 0.083 0.136

0.050 0.063 0.038 0.055 0.023 0.020 0.012 0.020 0.081 0.092 0.060 0.081

0.113 0.144 0.074 0.144

0.223 0.248 0.183 0.270

0.084 0.109 0.069 0.109

0.091 0.116 0.063 0.114 0.048 0.064 0.027 0.061 0.139 0.180 0.090 0.175

0.138 0.179 0.126 0.257 0.047 0.060 0.045 0.060 0.185 0.239 0.171 0.317

5 5 5 0 5 4 4 4 0 4 8 8 8 0 8

0.050 0.063 0.038 0.055 0.023 0.020 0.012 0.020 0.081 0.092 0.060 0.081

0.118 0.143 0.096 0.161 0.036 0.045 0.027 0.045 0.123 0.157 0.096 0.164

0.223 0.248 0.183 0.270 0.048 0.064 0.045 0.061 0.185 0.239 0.171 0.317

0.067 0.072 0.058 0.096 0.013 0.021 0.014 0.020 0.038 0.049 0.036 0.072

30 29 32 36 28 32 30 32 20 21 21 23

0.074 0.089 0.069 0.079 0.033 0.045 0.034 0.034 0.107 0.134 0.103 0.113

0.091 0.116 0.071 0.114 0.113 0.145 0.087 0.146

0.061 0.079 0.049 0.087 0.040 0.052 0.022 0.049

67 68 69 77 36 36 26 34

J5
Round Robin XXIII Laboratory Results

All Lab Report Page 5 / 6

Symbol

na 

nd 

ns 

italics Value calculated from reported results

n 

Min

Mean Average over all non-NIST reported values.

Max

SD

CV

Median 

eSD 

eCV 

Table Legend

Interpretation

Maximum non-NIST reported value.

Standard deviation over all non-NIST values.

Coefficient of Variation (% relative standard deviation): 100*SD/Mean

Median over all non-NIST reported values

Robust estimate of SD based on the adjusted median absolute difference from the median (MADe)

Robust estimate of CV, 100*eSD/Median

Not available (not reported by participant)

Not detected (not reported or reported as '0', 'not determined', etc.)

No sample (damaged in shipping or not provided by NIST)

Number of non-NIST laboratories reporting quantitative results

Minimum non-NIST reported value.

J6
Round Robin XXIII Laboratory Results

All Lab Report Page 6 / 6

Lab TR aT bC Label Definition

FSV-BA 4±2 1±1 1±3 Lab Participant code

FSV-BD 9±3 9±4 TR Total Retinol

FSV-BE -2±3 -2±3 aT a-Tocopherol

FSV-BF 5±4 3±4 18±15 bC Total or trans-b-Carotene

FSV-BG -1±3 -4±3 -6±18 % Bias (Mean ± SD) of individual serum biases

FSV-BH 5±6 -1±2 14±7

FSV-BI -1±1 -6±2 -10±2 Mean Average of (xi

-Mediani)/Mediani

FSV-BJ 0±1 2±1 4±7 SD Standard deviation of (xi

-Mediani)/Mediani

FSV-BK 19±9 3±4 xi Result for analyte in serumi

FSV-BL 12±4 -3±2 Mediani Median of non-NIST results in serumi

FSV-BM 0±2 7±7

FSV-BN -11±4 2±4

FSV-BP 6±6 56±10 48±58

FSV-BS -28±21 -71±12

FSV-BX -2±3 2±3

FSV-BY 4±8 5±5 9±9

FSV-BZ -10±6

FSV-CA 7±3 2±2

FSV-CH 11±1 10±4 9±10

FSV-CJ -24±2 -6±6 -26±4

FSV-CK 29±10 17±6 -58±14

FSV-CM -7±6

FSV-CO -4±2 -3±1 7±8

FSV-CP -24±7

FSV-CT -5±7 -10±7

FSV-CU -10±5 -5±1 14±16

FSV-CV -1±3 -11±5 -2±8

FSV-CX -18±4 -6±4

FSV-CY -1±4 0±4 5±9

FSV-DE 1±2

FSV-DG 1±1 2±2 -7±9

FSV-DH 8±17 15±18 13±16

FSV-DL -6±3 -16±4 -30±9

FSV-DM -8±1 6±20 5±10

FSV-DO 2±6 -18±19 14±49

FSV-DW -23±14

FSV-DY 6±5 2±2

FSV-EA -12±4 -19±15 -34±6

FSV-EB 0±5 1±1 -10±16

FSV-EF 4±6 1±2

FSV-EI 21±6 -5±34 -9±14

FSV-EJ -27±8 9±2 -51±32

FSV-EV 14±8 4±2

FSV-EY -1±1 9±2

FSV-FL -2±2 -4±3 10±10

FSV-FM -24±3 -12±18 -42±8

FSV-FR 1±1 5±2 7±5

FSV-FS -20±18 0±6 33±49

FSV-FU 56±14 -16±1

FSV-GB 7±3 -2±2 14±16

NISTc 4±3 0±2 -1±10

%Bias Summary

The original analysis listed % Bias for each result for 

each serum calculated relative to the "Trimmed Core 

Lab Average" of that analyte in the serum. The 

summary values reported here are the (arithmetic mean 

± standard deviation) of each laboratory's reported 

results for the analyte estimated relative to each serum's 

median value.

The results for the serum extract 158 do not necessarily 

reflect a participant's measurement performance for 

serum samples. Therefore, these summary values are 

calculated using only results for the four serum samples: 

155, 156, 157, and 159.

J7