Final

— July 2, 2015

1 

## Significant New Alternatives Policy Program

Refrigeration and Air Conditioning Sector

Risk Screen on Substitutes in Heat Transfer

Substitute: Meth oxytridecafluoroheptene I somers

This risk screen does not contain Clean Air Act (CAA) Confidential Business Information (CBI) and, therefore, may be disclosed to the public.



### 1. INTRODUCTION

Ozone

- depleting substances (ODS) are being phased out of production in response to a series of dip lomatic and legislative efforts that have taken place over the past two decades, including the Montreal Protocol and the Clean Air Act Amendments of 1990 (CAAA). The U.S. Environmental Protection Agency (EPA), as authorized by Section 612 of the CAAA, adm inisters the Significant New Alternatives Policy (SNAP) Program, which identifies acceptable and unacceptable substitutes for ODS in specific

end

- uses based on assessment of their health and environmental impacts.

EPA’s decision on the acceptability of a substitute is based on the findings of a screening assessment of potential human health and environmental risks posed by the substitute in specific applications. EPA has already screened a large number of substitutes in many end

- uses within all of the ma jor ODS

- using sectors, including refrigeration and air conditioning, solvent cleaning, foam blowing, aerosols, fire suppression, adhesives, coatings and inks, and sterilization. The results of these risk screens are presented in a series of Background Docu ments that are available in EPA's docket.

The purpose of this risk screen is to supplement EPA’s Background Document on the refrigeration and air conditioning sector (EPA 1994) (hereinafter referred to as the Background Docu ment). This risk screen evaluates the potential use of [ ]

. M ethoxytridecafluoroheptene isomers is a mixture of structural and stereo isomers, represented as [ ] . The exact structures of the remaining one percent of isomers are

unknown. This substitute is hereinafter referred to as methoxytridecafluoroheptene isomers (MPHE) and is proposed as a substitute in non

- mechanical heat transfer

. Table 1 presents information on the composition of the proposed substitute.

Table 1

. Composition of MPHE

Constituent Chemical
Formula CAS
Number a Range of
Concentration ( Weight Percent ) Typical
Concentration ( W

eight Percent )

Potential Impurities

a CAS Registry Numbers for MPHE are not available.

The potential risks associated with the use of substitutes in heat transfer have been examined at length in the Background Document. The reader is referred to this reference for a detailed discussion of the

me thodologies use d to conduct this risk screen. This ris k screen addresses flammability risk and assessesFinal

— July 2, 2015

2 the impact of potential releases of MPHE to the atmosphere. Additionally, occupational exposure and

end

- use exposure assessments were performed to ensure that manufacture and application of the proposed substitute in the proposed end

- uses do not pose unacceptable risk s to workers. Lastly, general population exposure was assessed to ensure that the proposed substitute does not pose an unacceptable r is k to the population at large.

Section 2 of this report summarizes the results of the risk screen for the proposed substitute listed in Table

1

. The r emainder of the report is organized into the following sections:

• Section 3 : Atmospheric Assessment

• Section 4 : Volatile Organic Compound Assessment

• Section 5 : Potential Health Effects

• Section 6: Flammability Assessment

• Section 7 : Occupational Exposure Assessment

• Section 8 : End

- Use Exposure Assessment

• Section 9 : General Population Exposure Assessment

• Section 10 : References



### 2. SUMMARY OF RESULTS

MPHE is recommended for SNAP approval as a heat transfer fluid in the non

- mechanical heat transfer

end

- use

. EPA's risk screen indicates that the use of the proposed substitute will be less harmful to the atmosphere than the continued use of ODS and other commonly used refrigerants, as it is less harmful to the ozone layer, h as lower climate impact, and a shorter atmospheric lifetime (ALT)

. MPHE is regulated

as a volatile organic compound (VOC) under CAA regulations (40 CFR 51. 1 00 (s) )

. Based on analysis of potential MPHE emissions , VOC s from the u se of MPHE in heat transfer ar e not anticipated to result in greater impacts on local air quality in the United States than other available options. R eleases of the proposed substitute should be controlled using absorbent material, and vapor in

- air detection systems

should be used to further minimize potential exposures , as MPHE is highly volatile. B ased on the characteristics of the proposed substitute and the proper precautions listed in the manufacturer’s safety

data sheet ( SDS ) for MPHE

, the proposed substitute is not expected to p ose a flammability risk during

manufacture or end

- use (see Section 6)

. Given that personnel will receive proper training, a ppropriate personal protective equipment (PPE) ( e.g., respiratory protection, goggles, impervious gloves, chemical

resistant clothing and boots) will be available

, and proper procedures according to the SDS will be used during manufacturing, end

- use, and disposal, no significant toxicity risk to workers, end

- users, or the general population is anticipated.



### 3. ATMOSPHERIC ASSESSMENT

Th is section presents an assessment of the potential risks to atmospheric integrity posed by the us e of

MPHE as a heat transfer fluid

. The ozone depletion potential (ODP)

, global warming potential ( GWP )

, ALT of the proposed substitute are presented in Table 2

. The proposed substitute is substantially less harmful to the ozone layer, has less climate impact, and a

shorter ALT when compared to CFC

- 113

, methyl chloroform , HCFC

- 225ca, and HCFC

- 225cb . In addition, MPHE also has lower climate impact and a shorter ALT than those predicted for other substitutes examined in the Background Document, as well as commonly utilized substitute s
, such as

HFC

- 365mfc and HFC

- 245fa

. Thus, EPA believes that use of MPHE would result in substantially less harm to the climate and ozone layer than the continued use of ODS and other commonly used ODS

substitutes

.Final

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3 Table 2

. Atmospheric Impacts of MPHE Compared to Other Heat Transfer Fluids

Chemical Ozone Depleting Potential (ODP) a Global Warming Potential (GWP) b Atmospheric Lifetime in Years (ALT) b

MPHE

CFC

- 113 0.8 5 6

, 1 30 85

HCFC

- 123 0 .01 77 1.3

HCFC

- 22 0.05 1,810 12

HFC

- 236fa 0 9,810 240

HFC

- 245fa 0 1,030 7.6

a WMO (2011)

b IPCC 4 th Assessme nt Report (Forster et al. 2007)



### 4

. VOLATILE ORGANIC COMPOUND ASSESSMENT

MPHE is regulated as a VOC under CAA regulations (40 CFR 51.100(s))

. Through regulations and standard industry practices , emissions of MPHE should be controlled.

The submitter intends to manufacture MPHE as a heat transfer fluid in new and retrofit non

- mechanical heat transfer systems. A n assessment was performed to compare the annual VOC emissions from the use of MPHE in the heat transfer end

- use to other anthropogenic sources of VOC emissions. Assuming the submitter’s maximum anticipated annual production of MPHE for the proposed end

- use is entirely released (in the absence of controls), approximately [ ] MT of VOCs would be emitted. This is approximately equal to [ ] percent of all anthro pogenic VOC emissions in the United States in 201 4 ; 1 however, the release of total production for the proposed end

- use of MPHE is extremely unlikely to occur. Thus, an add itional analysis was performed to determine more realistic annual VOC emissions

from the use of MPHE in non

- mechanical heat transfer applications.

Assuming the average annual release rate of two percent for chillers, 2 approximately [ ] MT of VOCs would be expected to be released into the atmosphere annually. This amount is appr oximately [ ] percent
of all anthropogenic VOC emissions in the United States . As the calculated annual releases of MPHE from this analysis are several orders of magnitude lower than other anthropogenic sources of VOC

emissions , and as VOC emissions from manufacture are anticipated to be controlled through standard industry practices, EPA believes that the environmental impacts of MPHE as a substitute in non

mechan ical heat transfer is not considered a significant concern.



### 5

. POTENTIAL HEALTH EFFECTS

To assess potential health risks from exposure to this proposed substitute, EPA identified the relevant toxicity threshold values for comparison to modeled exposure concentrations for different scenarios. The

submitter indicates MPHE has an acceptable exposure limit (AEL) of [ ] ppm. The AEL represents the

maximum 8

- hour time weig hted average (TWA) at which a worker can be exposed regularly without

adverse effects. A n AEL of 625 ppm was i ndependently developed for MPHE based on a review of available toxicity studies. EPA’s determination of a 625 ppm AEL and summary of available tox icity studies can be found in Appendix A

. Because it is the more conservative value, the submitter’s AEL of [ ] ppm was used in exposure assessments in this risk screen.

According to the SDS

, exposure to MPHE through ocular or dermal absorption, inhalation , or ingestion may be harmful. If MPHE is inhaled, ingested, or absorbed through skin or eyes in large amounts, the affected person(s) should adhere to the first aid measures provided in the SDS . In the case of ocular
exposure, t he SDS for MPHE recommends that person ( s ) should immediately flush their eyes, including

1 Based on the 2014 annual total VOC emissions for the United States (i.e., approximately 17.1 x 10 6 MT) as reported in the National Emissions Inventory (EPA 2015).

2 Leak rate for chillers from EPA’s Vintaging Model ( VM IO file_v4.4_10.13.14 ).Final

— July 2, 2015

4 under their eyeli d s, with water for 15 minutes . Medical attention should be sought if irritation persists ( DuPont 2012)

. In the case of dermal exposure to MPHE , the SDS for MPHE recommends that person(s) should immediately remove all contaminated clothing to avoid irritation and wash the affected area with

warm water

. If high concentrations of MPHE are inhaled

, the SDS recommends person ( s ) should be immediately

removed from the contaminated space and exposed to fresh air. The SDS recommends that if breathing is difficult, artificial respiration and/or oxygen may be necessary , and medical attention should be so ught. In case of ingestion, the SDS recommends the person ( s ) consult a physician immediately, and refrain from inducing vomiting without medical advice

, as there is a greater hazard associated with aspirating MPHE into the lungs if vomiting is induced.

EPA’s review of the human health impacts of this proposed substitute is c ontained in the public docket

for this decision. These risks and procedures after exposure are similar for other common heat transfer

fluids

. The potential health effects of MPHE can be minimized by following the exposure guidelines and

ventilation and PPE recommendations outlined in the SDS for MPHE and this risk screen

.



### 6. FLAMMABILITY ASSESSMENT

MPHE is considered to be non

- flammable ( DuPont 2012 ). Based on this classification, manufacture, servicing, and use of MPHE is not expected to present a flammability risk in heat transfer applications.



### 7. OCCUPATIONAL EXPOSURE ASSESSMENT

[ ]

. Using EPA’s Chemical Screening Tool for Exposures and Environmental Releases ( ChemSTEER )

, 3 the submitter provided estimated exposures during [ ]

. Neither the American Conference of Industrial Hygienists (ACGIH) nor the American Industrial Hygiene Association (AIHA) recommended short

- term exposure limits (STELs) for MPHE

. As a result, a STEL for MPHE was developed by EPA based on the

submitter’s AEL of [ ] ppm. The STEL was estimated as three to five times the AEL, which is an established method of estimating a short

- term exposure limit by ACGIH. The results of the submitter’s
exposure assessment are presented in Table 3.

Table 3

. Modeled Occupational Exposure during [ ]

Activity Time of Potential Exposure (hours /day ) Measured Exposure
Concentration ( ppm ) Exposure Limit (ppm)

a EPA

- derived STEL

b 8

- hour AEL

As sh own in Table 3, exposure during [ ] activities are significantly less than the STEL. Furthermore, worker exposure [ ] is expected to be significantly less than the 8

- hour AEL. If one worker were to perform all activities listed in Table 3 in an 8

- hour workday, the combined daily exposure would be [ ] ppm per day (DuPont 2012). This estimate is lower than the AEL and does not pose a concern for occupational exposure during all four activities over an 8

- hour workday.

Because ChemSTEER does not consider engineering controls such as local exhaust ventilation, exposure

3 EPA’s Chemical Screening Tool for Exposures and Environmental Releases (ChemSTEER) is a computer

- based software program used to estimate workplace exposures and environmental releases for chemicals manufactured and used in industrial/commercial settings.Final

— July 2, 2015

5 levels are expected to be lower than the results presented in Table 3. MPHE is not expected to pose a risk to workers during [ ] when the engineering controls and PPE guidelines consistent with the Occu pational Safety and Health Administration (OSHA) are followed

. In the case of large releases of MPHE

, the proposed substitute should be collected on absorbent material ( e.g ., rags, saw dust, vermiculite, spill pillows, cellulose products ) and transferred to steel drums for recovery or disposal to prevent leakage into sewers, waterways, or low lying areas, as recommended by

the SDS . Once the proposed substitute is adequately removed and clean

- up residues are disposed of in

accordance with the SDS , normal ventilation for standard manufacturing procedures and vapor in

- air detection systems should be used to further minimize potential exposures.

EPA believes that because proper handling and disposal guidelines are followed in accordance with good industrial hygiene and manu facturing practices and the substitute’s SDS , there is no significant risk to workers during the manufacture of MPHE

. See the following section, “End

- Use Exposure Assessment” for a discussion of exposure to workers using MPH E during heat transfer applications.



### 8

. END

- USE EXPOSURE ASSESSMENT

MPHE is proposed for use as a heat transfer fluid in new and retrofit non

- mechanical heat transfer
systems ( DuPont 2012). End

- use exposure is limited to [ ] . The potential exposures to workers during

these end

- use activities are examined in the sections that follow.

8.1 End

- Use Exposure at Charging, Servicing, and Draining for Reclamation

Minimal worker exposure is expected during use of MPHE in the proposed heat transfer applications;

h owever, the submitter notes various environmental releases may occur due to [ ]

. The submitter anticipates exposure to MPHE during [ ]

. The submitter modeled the exposure scenarios

u sing ChemSTEER

. The estimated exposure concentrations during [ ] are presented in Table 4.

Table 4

. Modeled End

- Use Exposure for MPHE in Heat Transfer (ppm)

Activity Time of Potential Exposure (hours /day ) Measured Exposure Concentration (ppm) Exposure Limit (ppm)

a EPA

- derived STEL

b 8

- hour AEL

The average anticipated exposure concentrations for MPHE during [ ] are significantly below the ST EL

for MPHE ( i.e., 1,500 ppm). Furthermore, t he combined daily exposure (i.e.

, if a worker were to perform

all the activities in an 8

- hour workday ) would be appr oximately [ ] ppm per day (DuPont 2012) . This

estimate is lower than the AEL, and does not pose a concern for exposure during all end

- use activities

listed in Table 4 o ver an 8

- hour workday.

EPA believes that because proper handling and disposal guidelines are followed in accordance with good industrial hygiene and manufacturing practices, and the substitute’s SDS , there is no significant risk to workers during charging, servicing, or draining of MPHE

.

8. 2 End

- Use Exposure at Recovery

This section examines the potential end

- use exposure during [ ] of the heat transfer fluid.

The results of the submitter’s exposure analysis were modeled with ChemSTEER and compared to the

STEL for MPHE . The resu lts of the ChemSTEER model are presented in Table 5.Final

— July 2, 2015

6 Table 5

. Modeled End

- Use Exposure for MPHE during [ ] (ppm)

Activity Time of Potential Exposure (hours/day) Measured Exposure Concentration (ppm) Exposure Limit (ppm)

a EPA

- derived STEL

b 8

- hour AEL

As shown in Table 5, potential exposur es during [ ] are significantly below the ST EL. Exposure to MPHE during [ ] over an 8

- hour period is also significantly less than the AEL of [ ] ppm for MPHE

. Furthermore, s ignificant exposure to MPHE is especially not expected during [ ] , as the fluid is

contaminated with an average concentration of [ ] percent MPHE . The combined daily exposure is

measured at [ ] ppm, which is also significantly lower than the AEL, an d conse quently does not pose a concern for exposure during [ ]

. In addition, t he estimated exposures were derived using conservative assumptions, and do not take into account the use of ventilation. These types of systems are typically serviced by trained person nel using proper industrial hygiene techniques and using proper engineering controls (e.g., local ventilation, refrigerant detectors, and alarm systems). Thus, EPA does not believe that recovery of MPHE present s a significant concern to workers.

Given that MPHE in the non

- mechanical heat transfer end

- use is not expected to result in signific ant

exposure , and because end

- users of MPHE will follow appropriate equipment guidelines and environmental measures, such as employment of capture and destruction technologies , EPA does not

believe that MPHE presents a significant risk to workers at end

- use.



### 9

. GENERAL POPULATION EXPOSURE ASSESSMENT

This section screens potential risks to the general population from exposure to releases of MPHE to
ambi ent air, surface water, and solid waste. Because the proper safety and disposal precautions, as listed in the remainder of this section, are followed, MPHE is not expected to cause a significant risk to the environment and human health in the general popu lation when manufactured o r used for non

- mechanical heat transfer applications.

9. 1 Ambient Air

MPHE is highly volatile [ ] . A ccidental release measures, handling and storage, engineering controls, and waste disposal must be followed per the proposed substitute’s SDS to avoid significant releases to air.

Given that non

- mechanical heat transfer applications will take place indoors with good manufacturing practices, such as employment of capture and destruction technologies, re leases are not expected to result in significant exposure to the general population.

9 .2 Surface Water

Due to the near

- insolubility of MPHE in water [ ]

, MPHE is not likely to accumulate in surface water ( DuPont 20 12 ). For the proposed heat transfer applications , the product will be handled indoors with waste or accidental spills collected in steel drums or take n up with an absorbent material . Water

contaminated with MPHE should not be dumped into sewers, on the ground, or into any body of water, in

a ccordance with the SDS

.Final

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7 9 .3 Solid Waste

Although the constituents of MPHE are not listed as Resource Conservation and Recovery Act ( RCRA ) hazardous wastes, contaminated heat transfer fluid may display characteristics of hazardous waste ( e.g

. , ignitability , corrosivity, reactivity, toxicity) . A ny solid waste displaying these characteristics or having a specific waste code (e.g., F001) is considered to be a hazardous waste (40 CFR Part 261). Waste regulated as RCRA hazardous waste is subject to the requirements of the Subtitle C program (including storage, treatment, and disposal requirements), which EPA believes are sufficient to control human health and environmental risks. In addition, because solid materials used during end

- use or clean

- up procedures that may be absorbed with MPHE ( i.e., rags, vermiculite, and spill pillows ) are disposed of in accordance

with the MPHE SDS , EPA does not expect general population exposure to MPHE through solid waste to

occur.Final

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8 

### 10

. REFERENCES

Du Pont Chemicals & Fluoroproducts (now The Chemours Company ). 2012. Premanufacture Notice and Significant New Alternatives Policy Program Submission to the Environmental Protection Agency for Methoxytridecafluoroheptene Isomers to the United States Environme ntal Protection Agency. June 22,

2012.

EPA 201 5 . National Emissions Inventory Air Pollutant Emissions Trends Data. Accessed 1 3 March 201 5

. Updated March 201 5 . Available at: http://www.epa.gov/ttnchie1/trends/.

EPA 1994. Significant New Alternatives Policy Technical Background Document: Risk Screen on the

Use of Substitutes for Class I Ozone

- depleting Substances: Refrigeration and Air Conditioning

. Stratospheric Protection Division. March, 1994.

Forster, P., V. Ramaswamy, P. Artaxo, T. Berntsen, R. Betts, D.W. Fahey, J. Haywood, J. Lean, D.C. Lowe, G. Myhre, J. Nganga, R. Prinn, G. Raga, M. Schulz and R. Van Dorland. 2007. Changes in Atmospheric Constituents and in Radiative Forcing. In: Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change [Solomon, S., D. Qin, M. Manning, Z. Chen, M. Marquis, K.B. Averyt, M.Tignor and H.L. Miller (eds.)]. Cambridge Un iversity Press, Cambridge, United Kingdom and New York, NY, USA.

WMO (World Meteorological Organization), 2011. Scientific Assessment of Ozone Depletion: 2010

, Global Ozone Research and Monitoring Project

— Report No. 52, 516 pp., Geneva, Switzerland.Final

— July 2, 2015

9 

## Appendix A

Determination of an Acceptable Exposure Limit ( AEL ) for MPHE

Recommended AEL: 625 ppm (8

- hour time

- weighted average)

Basis and Endpoints: NOAEC 4 : 2,500 ppm (dehydration; clinical chemistry; microscopic changes in the teeth)

Critical Study: 28

- day inhalation (whole body) study (DeLorme 2014)

Doses: 0; 1,000; 2,500; 5,100 ppm

NOAEC: 2,500 ppm

HEC 5 : 2,500 ppm x 6 hr/d / 8 hr/d = 1,875 ppm

Uncertainty Factors: 3

– animal to human extrapolation

4 No Observable Adverse Effect Concentration (NOAEC)

5 Human Equivalent Concentration (HEC)Final

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10 

## I. Introduction

MPHE [ ] has been proposed as a replacement for CFC

- 113, HCFC

- 141b, HCFC

- 225ca/cb , and methyl chloroform in many solvent cleaning uses, as well as in carrier fluid and heat transfer fluid applications. Several studies assessing the potential toxicity of MPHE have been conducted, including the following:

• An acute inhalation study in r ats;

• a sub

- acute (28 day) inhalation study in rats;

• four genotoxicity studies in bacterial and mammalian cells; and

• four oral (gavage) studies in rats.

The results of these studies are discussed in the following sections to inform an assessment of the potential health risks from human exposure to the study compound in the intended end

- uses.



## II. Summary of Toxicity Studies

The toxicological studies reviewed for the determination of an AEL for MPHE are summarized below in Table 1. The toxicity database is rather comprehensive, but is focused primarily on gavage (oral) exposure studies, which are of limited usefulness for the determination of an occupational AEL based on the expected inhalation expos ures (as discussed in the latter portion of Section IV).Final

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11 

### Table 1. Select MPHE Toxicological Studies

a All values NOAEL/NOAEC unless otherwise reported; NOAEL/NOAEC = No observed adverse effect level/concentration (commonly used for oral or dermal/inhalation exposures) ; wt

– weight Principal Studies Concentrations NOAEC (L) a Effects

Acute (inhalation; whole body; rat) 0; 2,000; 5,000; 9,700; 15,000 ppm LC 50 >15,000

ppm Anesthetic effects at ≥5,000 ppm including 

### ↓ activity 10 min. into exposure; rats were motionless at 45 min. into exposure.

28

- day (inhalation; whole body; rat) with 14

- day

recovery 0; 1,000; 2,500; 5,100 ppm 2,500 ppm Diuresis

- induced dehydration; chang es in clinical chemistry parameters; degeneration of tooth epithelium in 1 male at 5,100. Adaptive liver effects

acute (oral; female rat) 0; 5,000 mg/kg LC 50 >5,000 mg/kg No clinical findings or gross pathology.

28

- day (oral; rat) 0; 250; 500, 1,000 mg/kg 1,000 mg/kg Sig. ↑ absolute, relative liver wt in males and females; sig. ↑ absolute, relative

kidney wt in males. Hepatocellular hypertrophy in 2/10 males at 1,000 mg/kg; considered adaptive effect. No histopathology in kidneys in males. Some clinical chemistry changes at ≥500 mg/kg.

28

- day with FOB (oral; rat) 0; 100; 250; 1,000 mg/kg 1,000 mg/kg Sig. ↑ absolute, relative liver wt in males. Hepatocellular hypertrophy in both sexes at 250 and 1,000 mg/kg; considered adaptive
effect.

90

- day (oral; rat) with 30

- day recovery 0; 250; 500, 1,000 mg/kg Not identified (males); 1,000 mg/kg (females) Evidence of chronic progressive nephropathy in males at all doses, including 7/10 control animals; no similar

effects in females. Sig. ↑ absolute,
relative liver weights in both sexes at all
doses. Developmental toxicity (oral; rat) 0; 250; 500; 1,000 mg/kg 1,000 mg/kg No exposure

- related adverse effects.

Supporting Studies Doses NOAEL Effects

In vitro bacterial reverse mutation assay ≤ 5,000 μg/plate 5,000 μg/plate Negative

In vitro chromosomal aberration assay , CHL
cells ≤ 3621 μg/mL 3621 μg/mL Negative

In vivo micronucleus 0, 500; 1,000; 2,000 mg/kg 2,000 mg/kg Negative

In vivo micronucleus 0, 1,000; 2,500; 5,100 ppm 5,100 ppm NegativeFinal

— July 2, 2015

12 

## III. Development of the AEL for MPHE

The body of data indicates that MPHE induces anesthetic effects in rodents at concentrations ≥ 5,000 ppm in inhalation exposures lasting 4 hours; however, no neurotoxic effects were noted in the 28

- day inhalation study. Toxicity from repeated inhalation ex posure is limited to diuresis

- induced dehydration, increased serum levels of albumin, globulin and total proteins, and decreased body weight, in male rats only at 5,100 ppm. Further, one male rat at 5,100 ppm exhibited degeneration/atrophy of the ameloblas tic epithelium (the enamel

- producing cells of the teeth). Hepatocellular hypertrophy was noted in both sexes at 5,100 ppm and in males at 1,000 and 2,500 ppm, but this effect is due to an adaptive increase in metabolic enzymes and commonly occurs in respon se to many xenobiotics. Thus, it was not considered adverse. Oral toxicity appears to be limited to adaptive effects in the livers and kidneys of primarily male rats. No effects in the kidneys other than increased absolute and relative weight were presen t in the 28

- day gavage study, whereas, kidney lesions (i.e., chronic progressive nephropathy [CPN]) were present in

the 90

- day study.

Because of the high incidence of CPN in control male rats in the 90

- day gavage study, however, the effect

cannot be ascr ibed specifically to oral exposure to the study compound. The problematic kidney data limits the usefulness of the oral studies for establishing an occupational exposure limit. Another limitation of the studies is that the oral (gavage) pathway used to ex pose the rodents to MPHE is not relevant for worker exposure. Although the submitter argues that inhalation toxicity is unlikely to be a significant pathway of exposure (DuPont 2013), the vapor pressure for the compound ( [ ] mm Hg) and boiling point ( [ ] o C ) are comparable to those of perchloroethylene (18.6 mm Hg; 121.1oC), and the compound is generally classifiable as volatile or likely to be volatile (Mackay et al. 1993). It is reasonable to expect

that MPHE will enter the vapor phase during vapor degre asing and if it is used as an aerosol solvent. Further, exposure to high concentrations identified in the acute inhalation exposure study resulted in clinical signs indicative of anesthetic effects. This contrast between the acute inhalation study and the oral studies indicates that there are differences in activity in the compound between exposure routes, and thus the oral studies will not serve as a basis for development of an exposure limit that is protective of occupational health. For this reason, the 28

- day inhalation exposure study has been chosen as the appropriate toxicity study.

ICF calculated the AEL using the following equations:

2,500 ppm x (6 hr/8 hr) = 1,875 ppm

1,875 ppm x 1/ 3 = 625 ppm

A HEC was determined by adjusting the identified NOAEC of 2,500 ppm in the 28

- day inhalation study by the ratio of rat exposure duration per day to that of an occupational worker (6 hours/8 hours). No further exposure duration adjustment was necessary. A t otal uncertainty factor (UF) of 3 was applied to account for pharmacodynamic differences between rats and humans as the guidelines for developing

Reference Concentration ( RfC ) values ( U.S. EPA, 1994 ) were followed.Final

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13 

## IV. Principal Toxicity Studies

Inhalation Exposure

Acute Study

In the acute study (Kegelman 2011), five young adult Sprague

- Dawley rats per sex were exposed via inhalation (whole

- body exposure) to MPHE in measured concentrations of 0 ; 2,000; 5,000 ; 9,700 ; or 15,000 ppm for 4 hours. During the exp osure
, the rats were reportedly assessed for response to stimuli (startle response) three times. Rats were sacrificed 14 days following exposure and assessed for gross pathological effects; rats in control and 15,000 ppm groups were assessed for histopatho logy in the respiratory tract, livers, and kidneys.

According to the study summary and discussion sections, 5/5 rats of both sexes at 9,700 ppm exhibited decreased activity within 10 minutes of the start of exposure, and within 30 minutes of exposure, th e rats
became motionless and remained motionless throughout the rest of the exposure period. The only exception is that the rats did exhibit a normal startle response when challenged. All male and female rats at 15,000 ppm exhibited increased activity leve l from 10 minutes until 45 minutes of exposure, which included the following: rapid head

- bobbing and ear twitching, chewing motions, excessive grooming, and an increased startle response. All rats were motionless within 20 minutes of the start of exposure . The anesthetic symptoms were gone 5 minutes (9,700 ppm) or 6 minutes (15,000 ppm) post

- exposure, indicating that the study compound was exhaled quickly and did not persist in the bloodstream to cause any adverse clinical signs (DuPont 2013a). No abnormal clinical signs were reported during the 14

- day recovery period in any exposure group.

Four of five male rats at 9,700 ppm, 5/5 male rats at 15,000 ppm, and 2/5 female rats at all levels exhibited weight loss after the first day of exposure (≤ 10 grams), but normal weight gain was reported during the recovery period. As food consumption was not measured, it is not possible to determine if this minimal weight loss was a result of the animals avoiding their food.

None of the rats exhibited adverse signs u pon gross necropsy and no exposure

- induced signs of toxicity were noted in the 15,000 ppm

- group animals as compared to controls. It is notable that 2/5 control males exhibited minimal CPN, as did 1/5 males at 15,000 ppm. The incidence of CPN in the other groups, if any, is not reported because tissues from those animals were not evaluated microscopically.

Subacute (28

- day) Study

In a 28

- day whole body inhalation study (DeLorme 2014), male and female CD ® rats (20/sex) were exposed to: 0; 1,000; 2,500; and 5,100 ppm of the study compound for 6 h ou rs per day, 5 days per week

, for 4 weeks (21 exposures). Blood samples were taken from 5 rats/sex/exposure group following the

fourth exposure and again at the end of the exposure period for analysis of micronucle i induction (discussed in the Genotoxicity section). Following the exposure period, 10 male and 10 female rats in each group were allowed a 14

- day recovery period to assess the reversibility of any observed adverse effects. Blood and urine samples were ta ken from the remaining 10 rats/sex in each exposure group for hematology, clinical chemistry and urinalysis assessment; the rats were then sacrificed for gross necropsy and organ analysis.Final

— July 2, 2015

14 The study followed harmonized guidelines and appropriate endpoints were measured. Exposure to

MPHE did not result in early mortality

, moribundity or adverse clinical signs. 6 Further, no adverse findings were noted in the measured ophthalmological or neurobehavioral endpoints, including forelimb and hindlimb grip streng th, motor activity assessment, and functional observational battery measurements. No changes were noted in any exposed groups in body weight gain, food consumption, or hematology. High

- concentration males did exhibit a slight (11.3%) but significant reduct ion in body weight following the final exposure compared to controls. Plasma fluoride was increased significantly in

mid

- and high

- concentration males and females and urinary fluoride was increased significantly in all exposed rats. Plasma fluoride levels returned to near control levels following the 14

- day recovery period; urinary fluoride levels had decreased, but not completely returned to pre

- exposure levels following recovery. Male rats at 5,100 ppm exhibited diuresis (increased urine volume and decr eased urine specific gravity), as well as increased serum albumin, globulin, and total protein levels, and decreased body weight. The study authors considered the increased serum protein load and the decreased body weight to be secondary to the dehydratio n resulting from fluoride

- induced increased urine output; ICF agrees with

this assessment. Diuretic effects were also noted in low

- and mid

- concentration males and in mid

- and high

- concentration females; slight increases in serum proteins in males at 1,00 0 and 2,500 ppm were also seen, but the changes were minimal and were not coincident with body weight decrements. Thus, although exposure related, the changes were not deemed adverse. Further, the observed changes in urinalysis and clinical chemistry par ameters were reversible following the recovery period.

Adaptive changes related to compound exposure were noted in the liver, as well as in the larynx :

• mild hepatocellular hypertrophy in all high

- concentration rats,

• minimal hypertrophy in

o 3/10 males at 1,000 ppm

o 10/10 males at 2,500 ppm, and

o 10/10 females at 5,100 ppm;

• minimal hyperplasia/squamous metaplasia of the larynx in

o 8/10, 6/10, and 10/10 exposed males in low

- , mid

- , and high

- concentration groups at

necropsy,

o 5/10, 8/10, and 10/10 exposed fem ales in low

- , mid

- , and high

- concentration groups at

necropsy,

o 3/10 low

- and 4/10 high

- concentration males at recovery, and

o one high

- concentration female at recovery;

• minimal goblet cell 7 hyperplasia in the anterior section of the nose at terminal and recovery necropsy in

o 8/10, 8/10, and 8/10 low

- , mid

- , and high

- concentration males, and

o 5/10, 8/10, and 7/10 low

- , mid

- , and high

- concentration females.

Liver weights were slightly but si gnificantly increased in all exposed male groups and in high

concentration females, resulting from the hypertrophy. These increases were partially resolved after the

14

- day recovery period: males at 2,500 and 5,100 ppm and females at 5,100 ppm still exhibi ted increased liver weights compared to controls, but the values had decreased over the preceding 14 days. Interestingly, 2,500

- p p m recovery females had significantly (8%) increased liver weights compared to controls, but this value was unlikely to be bio logically relevant as it was not present at the end of exposure. No other organ weights were affected by exposure.

6 One 1

, 000

- ppm male was sacrificed on day 27 due to poor health; based on necropsy findings, the animal's condition was

attribu ted to urogenital tract inflammation that was not related to exposure.

7 Goblet cells are epithelial cells that produce mucins (components of mucus).Final

— July 2, 2015

15 A single male at 5,100 ppm exhibited degeneration/atrophy of ameloblastic epithelium 8 at recovery, believed to be due to fluoride exposure i n the study compound. Very slight lamination of dentin was noted in one male at 2,500 ppm and in 2/10 males at 5,100 ppm at recovery, and incomplete decalcification of enamel/dentin in 4/10 males at 2,500 ppm and 5/10 males at 5,100 ppm. No exposed

female s exhibited these endpoints. The incomplete decalcification is indicative of exposure only and is an artifact of tissue processing. The slight lamination of dentin was not considered adverse and occurred in only one male at 2,500 ppm. The NOAEC was ident ified as 2,500 ppm by the study author, based on

diuresis

- related effects (increased serum proteins, dehydration and weight loss) and microscopic effects in

the enamel

- producing cells of the teeth in 1 male at 5,100 ppm.



### Oral Exposure

The remaining roden t toxicity studies all utilized the oral (gavage) exposure pathway because, according to the submitter, significant repeated inhalation exposures were unlikely and it would be difficult to

achieve airborne concentrations of MPHE given its relatively low va por pressure (DuPont 2013b). Four gavage studies were conducted: a limit dose (acute) study, two subacute ( 28

- day ) repeat exposure studies, one subchronic study, and a developmental study.

Acute exposure

An oral limit

- dose assay in young adult Sprague

- Dawley rats (5 females) was performed to determine the limits of acute toxicity (Durando 2009). In this study, only female rats were used because the study authors claimed that females are "frequently more sensitive to the toxicity of test compounds than m ales." One female was initially given a single bolus dose of MPHE (neat) via gavage at 5,000 mg/kg. When this rat did not die prematurely, 4 additional females were dosed in the same manner. The animals were watched cage

- side for mortality and clinical s igns of toxicity within the first 30 minutes and during the

first several hours after dosing

. No behavioral challenges were performed as in the inhalation study, although it was noted that "particular attention was directed to observation of tremors, convu lsions, salivation, diarrhea, and coma." Gross necropsies were performed after 14 days of recovery, but no histopathology was done to determine that tissues were not affected. No adverse effects were reported and the rats satisfactorily gained weight (bod y weights were only measured at days 7 and 14, so subtle changes in body weight over 1

- 2 days would not have been evaluated in this study). Food consumption

was not measured. The LC 50 for females was determined to be >5000 mg/kg.

Subacute ( 28

- day ) Study

In the first 28

- day repeat exposure study (MacKenzie 2009), male and female young adult Sprague

Dawley rats (10/sex/dose) were exposed to the following doses of MPHE once daily, for 28 total days: 0 ; 250 ; 500 ; or 1,000 mg/kg. The exposure did not adversel y affect mortality, clinical signs of toxicity, food consumption or efficiency, body weight gains, hematology or clinical chemistry parameters, or gross necropsy. Significant increases in absolute and relative liver weights were noted in all dosed male and female groups, with an associated, small increase (2/10) incidence of minimal centrilobular hypertrophy at 1,000 mg/kg. No histological effects were noted in any dosed females. Significantly increased absolute and relative kidney weights were also seen in dosed male rats, but not in females; however, there was no associated kidney histopathology in these male rats. The liver effects were considered by the study authors to be the result of an adaptive response to the presence of a xenobiotic; ICF agrees b ecause of the lack of effects on liver cells (i.e., no adverse histopathology). There were incidental, small changes in some clinical chemistry parameters which were not considered adverse either because the changes were not consistently dose

- dependent or they did not correspond with tissue damage: increased serum globulin

8 Ameloblastic cells produce enamel in the tooth.Final

— July 2, 2015

16 and glucose in female rats at the top two doses, and decreases in select enzymes/compounds (sorbitol dehydrogenase, creatinine, inorganic phosphorus). The NOAEL for this study is identifi ed as 1,000 mg/kg.

In the second 28

- day repeat exposure study (Akahoshi 2010), 5 male and female Sprague

- Dawley rats

were administered doses of 0 ; 100 ; 250 ; and 1,000 mg/kg via oral gavage once daily for 28 consecutive days. Because certain parameters w ere not measured ( e.g., urinary analyses) this was not considered a guideline study. An additional 5 male and female control and high

- dose (1,000 mg/kg) rats were exposed identically to the main group animals, and were then maintained post

- dosing for 14 da ys to assess recovery. Exposure to MPHE did not affect clinical signs, parameters measured in a functional observational battery, mortality, food consumption, body weights, hematology, most clinical chemistry parameters, select urinalysis parameters, or gr oss necropsy findings.

In males at 1,000 mg/kg, significantly increased serum cholesterol and total phospholipids were noted at the end of exposure. This is most likely the result of stress to the liver as a result of increased metabolic activity, which is noted in the increased absolute liver weights in these males and the presence of mild centrilobular hepatocellular hypertrophy in 1/5 males at 250 mg/kg and 3/5 males at 1,000 mg/kg. Significantly increased liver weight relative to body weight was not ed at all three exposure doses in males, but in low

- and mid

- dose males, this is most likely the result of decreased mean body weights at the end of the study (compared to controls). Liver weights in exposed females were unaffected, but 1/5

and 2/5 females at 250 and 1,000 mg/kg respectively did exhibit mild centrilobular hypertrophy and 1/5 of the high

- dose females also exhibited fatty periportal hepatocyte degeneration with resultant mononuclear cell infiltration. Females and males at 250 mg/kg showed sig nificantly increased absolute and relative kidney weights, respectively, but these changes were not considered related to compound exposure as no other dose groups were affected. One high

- dose male showed histological evidence of accumulation of alpha 2

- m u globulin; this effect was not considered clinically relevant because it is male

rat

- specific and has no corollary in the human. In recovery animals, only 1,000

- mg/kg males still exhibited significantly increased absolute and relative liver weights. Liv er histopathology was resolved in high

- dose males after recovery. The NOAEL was identified by the study authors as 1,000 mg/kg as the adaptive effects were not considered adverse and were reversible; ICF agrees that because the effects noted in this study were similar to those noted in the first 28

- day study, the effects are indicative of adaptive response to a xenobiotic (i.e., increased cell size due to increased production of metabolizing enzymes) and are considered to be reversible and not adverse.Final

— July 2, 2015

17 Sub chronic ( 90

- day ) Study

In a subchronic oral study (Anand 2011), groups of male and female Sprague

- Dawley rats (15/group for controls and high

- dose animals, 10/group for intermediate groups) were given the following doses via gavage once/day, 5 days/week

, f or 13 weeks: 0 ; 250 ; 500 ; and 1,000 mg/kg. At the end of the 90

- day exposure period, 5 rats per sex at 0 and 1,000 mg/kg were maintained for 30 days post

- dosing to determine the reversibility of any observed effects.

The exposure to MPHE did not affect th e following measured parameters in any toxicologically significant way:

• mortality, 9

• clinical signs (including neurological parameters measured in a functional observation battery),

• ophthalmology,

• hematology or

• gross necropsy.

Changes in liver and kid ney weights and other measured parameters in exposed rats (main study) are provided below in Table 2. Mean terminal body weights of all male dosed groups were significantly lower than the controls (16%, 11%, and 9%, in the low

- , mid

- , and high

- dose groups respectively). Mean body weight values for the 250

- mg/kg

- day male group were consistently lower throughout the study (including prior to dosing at day 0); this may partly be the result of a significantly decreased food consumption (compared to controls) that occurred throughout the study except for days 42

- 56. Food consumption levels of the 500

- and 1,000

- mg/kg

- day males were comparable to controls throughout the exposure period. Overall mean body weight gains in the dosed groups were 76%, 83%, and 87%, respectively, of controls from day 0

- 91 (all values significantly decreased compared to controls). The study authors did not attribute decreased food consumption in the low

- dose males to exposure to MPHE

because the behavior did not follow a dose

- related trend; no potential mechanism for the decreased terminal body weights or deficits in overall body weight gain in all dosed male groups was offered in the study report. These deficits suggest a problem related either to the health of the male rats prior to the onset of exposure or to stress related to dosing methodology or treatment. Dosed females showed no changes in food consumption or efficiency compared to controls, and mean body weight gains were generally higher in dosed females than in controls, whic h are reflected in the mean terminal body weights (shown in Table 2).

9 One control male in the recovery group was found dead after intubation on day 85.Final

— July 2, 2015

18 Table 2. Selected Organ Weights in Male and Female Rats Exposed to MPHE

Absolute and Relative Organ Weights (g) and Effects

Exposure Dose, mg/kg

0 250 500 1,000

Male, number of rats (10) (10) (10) (10)

Mean terminal body wt. 596.0 500.8* 528.1* 549.1

Liver

Absolute wt. 15.956 19.424* 20.553* 23.066*
Relative wt. (to body wt.) 2.668 3.882* 3.900* 4.196* Hepatocellular hypertrophy

minimal 0 3 6 7

mild 0 0 0 1
Kidney

Absolute wt. 3.412 3.460 3.765 4.409*
Relative wt. (to body wt.) 0.573 0.694* 0.711* 0.801*

CPN, incidence 7 8 10 10

Severity, minimal 6 4 4 1

mild 1 4 6 9

Female, number of rats (10) (10) (10) (10)

Mean terminal body wt. 278.0 310.5* 288.4 291.1

Liver

Absolute wt. 7.314 9.127* 8.535* 8.833*
Relative wt. (to body wt.) 2.639 2.947* 2.953* 3.041* Hepatocellular hypertrophy

minimal 0 0 1 4

mild 0 0 0 0

Kidney

Absolute wt. 1.828 2.190* 2.053* 2.017*
Relative wt. (to body wt.) 0.660 0.708 0.712 0.695

CPN, incidence 1 1 2 0

Severity, minimal 1 1 2 0

Source: Anand ( 2011 )

* S tatistically significant at p<0.05.Final

— July 2, 2015

19 Absolute and relative (to body weight) liver weights were significantly increased in all dosed male and female rats. In addition, minimal to mild hepatocellular hypertrophy

, "characterized by an increase in the size of cells due to an increase in cytoplasmic volume

, " was present in a dose

- dependent manner in all exposed male groups and in females at the top two doses ( Anand 2011 ) . S erum cholesterol was significantly increased in male rats at the top two doses (46% and 74% higher than controls), and in the recovery males, cholesterol levels were still statistically higher (26%) than controls. The difference in the recovery males was smaller than in the main study, indicating that the liver damage was reversible. Absolute liver weights in 1,000

- mg/kg recovery males and females were not significantly increased over controls, but relative (to body weight) liver weights in 1,000

- mg/kg mal es were still significantly increased (see Table 3 below ). These changes indicate that the increased metabolic burden posed by

MPHE was having some cellular effect on the liver, which had not fully resolved at 30 days past the termination of exposure.

Table 3. Organ Weights and Incidence and Severity of Hepatocellular Hypertrophy in Livers and CPN in Kidneys of Recovery Rats

Finding Dose, mg/kg

0 1,000

Liver W eights (incidence of hypertrophy)
Absolute, male 15.001 (0) 17.457 (0)
Absolute, female 7.534 (0) 8.466 (0) Relative to body weight, male 2.413 2.963* Relative to body weight, female 2.488 2.780
CPN (Male) 3 4
Minimal 3 3

Mild 0 1

Source: Anand ( 2011 ) *statistically significant at p<0.05.

The absolute and relative kidney weights in treated males were all significantly increased compared to

controls in a dose

- related fashion, although the increased relative kidney weights were affected by the lower terminal body weights of the dosed male rats. Absolute, but not relative, kidney weight s of dosed female rats were also significantly increased compared to controls. CPN was noted in all male rats, and increased in severity in the dosed rats. CPN was also noted in female rats in the control, low

- and mid

dose groups, although the severity wa s "milder" than that observed in the males. CPN was not noted in control or high

- dose recovery females, but it was noted in a smaller number of control males (3/4; minimal severity) and 1,000

- mg/kg

- day males (4/5; 3 with minimal severity, 1 with mild sever ity). Because of the decreased number of recovery animals compared to main study animals (5 rather than 10) it is not possible to determine if the incidence of CPN was decreased, but the severity of the effect does appear to have decreased.

All dosed mal e rats exhibited significant increases in urinary micro

- total protein (83%, 186%, and 253%, respectively) compared to controls at the end of exposure, which corresponded to higher severity of CPN in these rats. There was also an increased incidence, not do se

- related, in granular casts in the urine

. Mean urine volume was increased and specific gravity decreased, in mid

- and high

- dose males (these last two endpoints were highly influence d by the values of one male rat in each group, and the values for the

rem aining rats in each group were within historical ranges). Mean urine volume and specific gravity were similar in control and high

- dose recovery males. Urinalysis parameters were not affected by treatment in

dosed females.Final

— July 2, 2015

20 The study authors note that CPN i s a common lesion in the aging rat, but can also be found in rodents as young as 2 months of age. The phenomenon is well

- known in 13

- week and 2

- year toxicity studies conducted by the National Toxicology Program (Travlos et al. 2007) and is associated with a high

- protein diet, NIH

- 07 (Seely and Hard 2008; Hard et al. 2009). It is noted that DuPont consistently uses in all its oral studies a diet with a protein content of 20%, which is intermediate between the old diet used by the NTP(NIH

- 07; 23% protein) a nd the new diet adopted in the 1990s ( NTP 2000 ; 14% protein) ( Hard et al. 2009). It is possible that the diet used by DuPont has a high enough protein content to contribute to the development of CPN in its male and female rats. As noted previously, a few male rats in the acute inhalation study exhibited signs of CPN at the age of 2 months (2/5 control rats, with minimal severity; 1/5 rats at 15,000 ppm, with minimal severity; no other dose groups were evaluated histologically). CPN was observed in a signif icant number of control and dosed male rats in the 90

- day oral study; the presence of CPN in 2 control rats and 1 dosed rat in the inhalation study indicates that there may have been a predisposition for CPN in this particular strain of rat, or the potenti al for developing CPN in a very short period of time as a result of diet or other factors.

The study report argues that no NOAEL was identified for male rats due to the presence of CPN in all male rat groups, but identifies a NOAEL for females at 1,000 mg/kg due to the absence of test

- substance

related effects. The author indicates that CPN is a kidney pathology unique primarily to the male rat with no known relevance to humans; the study author did, however, state that the increase in CPN severity was

believed to be an adverse effect related to MPHE exposure. Some scientists do believe that CPN has no relevance to human health risk assessment (Hard et al. 2009; Seely and Hard 2008), but others have disagreed with that viewpoint (Melnick et al. 2012). Nevertheless, the high incidence of CPN in control rats makes an assessment of the kidney as a target organ for MPHE impossible. As such, CPN is not considered a toxicologically relevant endpoint for humans, and it does not inform the AEL for this compound

.

Of greater importance for the development of the AEL, however, is the lack of relevance of oral exposure to an occupational worker. It is not believed that workers will be incidentally ingesting the study compound in any significant amounts. It is possi ble that like some perfluorinated alkanes which are also nephrotoxins, MPHE may be metabolized by glutathione conjugation, followed by ß

- lyase dependent biotransformation to reactive intermediates (Iyer and Anders 1997; Anders 2008). However, even if

metab olic studies were to indicate MPHE were metabolized in this fashion, the ß

- lyase metabolism pathway in humans is 10

- 30 fold less active than in rodents (Anders and Dekant 1998; Gentz and Malan 2001), suggesting that the effect in humans would be much less significant. As discussed previously, the inhalation pathway is the most relevant, and the 28

- day inhalation study performed was used to determine

the AEL.

Developmental Study

In a developmental assay, 22 time

- mated female Sprague

- Dawley rats were administered MPHE via oral gavage on gestation days (gd) 6

- 20 at doses of 0 ; 250 ; 500 ; and 1,000 mg/kg (Munley 2010). The dams were euthanized on gd 21 and the recommended reproductive a nd developmental parameters were evaluated. Exposure to the study compound did not affect any measured endpoint including those assessing maternal health and fetal development (e.g., fetal number or weight, as well as external, visceral, or skeletal alter ations). It was noted in the study that the percentage of male rat pups was statistically significantly increased at 500 and 1,000 mg/kg. The study authors indicated that the statistical significance was likely a result of the low ratio of males in the c ontrol group, and ICF agrees. Furthermore, ICF notes the following:

• no changes in pre

- or post

- implantation loss were observed across the exposed groups,Final

— July 2, 2015

21 • pup gender was established at conception,

• the dams were purchased already mated and at gd 1, 2, or 3, and

• the dams were not exposed to the study chemical until gd 6.

Other than selective loss of female pups, which the aforementioned data disprove, there is no other mechanism by which exposure to the compound during this developmental perio d could increase the percentage of male pups. In addition, the ranges of male pups in this study are comparable to those found

in more wide

- ranging analyses (Stump et al. 2012). Therefore, ICF believes that the sex ratio in this study was unaffected by exp osure to MPHE . The NOAEL was identified as 1,000 mg/kg by the study authors; ICF agrees.



## V. Supporting Genotoxicity Studies

The genotoxicity data for this study compound consist of a bacterial reversion assay, an assay evaluating

chromosomal aberration in Chinese hamster ovary cells, and two micronucleus assays in rat blood cells following repeated oral and inhalation exposures (Myhre 2009; Glover 2009; Myhre 2012; DeLorme 2014). All studies were determined to be negative, indicating that under the conditi ons used, MPHE and/or its metabolites are not believed to be mutagenic or clastogenic.Final

— July 2, 2015

22 

## VI. References

Akahoshi

, H. 2010. A 28

- day repeated dose oral toxicity study of MPHE in rats. Mitsubishi Chemical Medience Corporation for EI duPont de Nemours and Com pany, Wilmington, DE. 21 December 2010.

Anand

, S

. S. 2011. [ ] : Subchronic toxicity 90

- day gavage study in rats. E.I. du Pont de Nemours and Company, DuPont Haskell Global Centers for Health & Environmental Sciences, Newark, DE. 2 June

2011.

Anders, M.W. 2008. Chemical toxicology of reactive intermediates formed by the glutathione

- dependent bioactivation of halogen

- containing compounds. Chem. Res. Toxicol 21:145

- 159.

Anders, M.W., W. Dekant. 1998. Glutathione

- dependent bioactivation of haloal kanes. Ann Rev

Pharmacol Toxicol 38:501

- 537.

DeLorme

, M

. P. 2014. [ ] : Four

- week inhalation toxicity study with micronucleus evaluation in rats. E.I. du Pont de Nemours and Company, Newark, DE. 2 July 2014.

Durando

, J. 2009. [ ] : Acute oral toxicity

-- fixed dose procedure in rats. Eurofins Product Safety Laboratories Dayton, NJ for EI duPont de Nemours and Company, Wilmington, DE. 12 March 2009.

E.I. du Pont de Nemours and Company. 2013a. Additional information provided to SNAP from DuPont. 27 May 2013.

E.I. du Pont de Nemours and Company. 2013b

. Additional information provided to SNAP from DuPont. 11 February 2013.

Gentz, B.A. and T. P. Malan. 2001. Renal toxicity with sevoflurane: a storm in a teacup? Drugs 61:2155

- 62.

Glove r
, K

. P. 2009. [ ] : In vitro mammalian chromosome aberration test in Chinese hamster ovary cells. E.I. duPont de Nemours and Company, DuPont Haskell Global Centers for Health & Environmental Sciences, Newark, DE. 26 June 2009.

Hard

, G

. C

. , J. K. Johnson, and S. M. Cohen. 2009. A comparison of rat chronic progressive nephropathy

with human renal disease

-- implications for human risk assessment. Crit Rev Toxicol 39:332

- 346.

Iyer

, R

. A . and M.W. Anders. 1997. Cysteine conjugate ß

- lyase

- dependent biotransformation of the cysteine S

- conjugates of the Sevoflurane degradation product 2

- (fluoromethoxy)

- 1,1,3,3,3

- pentafluoro

- 1

- propene (Compound A). Chem Res Toxicol 10:811

- 819.

Kegelman

, T

. A. 2011. [ ] : Inhalation acute exposure with anatomic pathology evaluation in rats. E.I. duPont de Nemours and Company, DuPont Haskell Global Centers for Health & Environmental Sciences, Newark, DE. 22 February 2011.

Mackay D

. , W. Y. Shiu

, and K. C. Ma. 1993. Illustrated Handbook of Physical

- Chemical Properties and

E

nvironmental Fate for Organic Chemicals. Vol. III. Volatile Organic Chemicals . Boca Raton: Lewis Publishers, 1993.Final

— July 2, 2015

23 MacKenzie

, S

. A. 2009. [ ] : Repeated

- dose oral toxicity 28

- day gavage study in rats. E.I. duPont de Nemours and Company, DuPont Haskell Glob al Centers for Health & Environmental Sciences, Newark, DE. 16 July 2009.

Melnick

, R

. L

. , K. M. Burns, J. M. Ward, et al. 2012. Chemically exacerbated chronic progressive nephropathy not associated with renal tubular tumor induction in rats: an evaluation based on 60 carcinogenicity studies by the National Toxicology Program. Toxicol Sci 128:346

- 356.

Munley

, S

. M. 2010. [ ] : Developmental toxicity study in rats. E.I. duPont de Nemours and Company,

DuPont Haskell Global Centers for Health & Environmental S ciences, Newark, DE. 10 August 2010.

Myhre

, A. 2009. [ ] : Bacterial reverse mutation test. E.I. duPont de Nemours and Company, DuPont Haskell Global Centers for Health & Environmental Sciences, Newark, DE. 30 June 2009.

Myhre

, A. 2012. [ ] : Rat blood micronucleus test. E.I. duPont de Nemours and Company, DuPont Haskell Global Centers for Health & Environmental Sciences, Newark, DE. 11 January 2012.

Seely, J. C. and G. C. Hard. 2008. Chronic progressive nephropathy (CPN) in the rat: review of pathology and relationship to renal tumorigenesis. J Toxicol Pathol 21:199

- 205.

Stump

, D

. G

. , M. D. Nemec

, G . A. Parker

, et al. 2012. "Significance, reliability, and interpretation of developmental and reproductive toxicity" in Hood RD, ed: Developmental and Reproductive Toxicology, A Practical Approach, 3rd Edition. London: Informa Healthcare. 2012.

Travlos

, G

. S

. , L. Betz, and G. C. Hard. 2007. The influence of diet or route of exposure on chronic progressive nephropathy, kidney weight, bl ood urea nitrogen and creatinine concentrations in 13

- week toxicity studies for control F344/N male rats. Abstract. Toxicol Pathol 35:1.

United States Environmental Protection Agency (U.S. EPA). 1994. Methods for Derivation of Inhalation

Reference Concent rations (RfCs) and Application of Inhalation Dosimetry. U.S. Environmental Protection Agency, Office of Research and Development, Office of Health and Environmental Assessment, Washington, DC, EPA/600/8

- 90/066F.