# Patent

## Title
Means for treating synucleinopathies

## Abstract

The present invention relates to peptides or polypeptides for producing medicaments for preventing and/or treating synucleinopathies.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a National Stage of PCT/AT10/000303 filed Aug. 20, 2010 and claims the benefit of Austrian application A 1324/2009 filed Aug. 21,2009.
FIELD OF THE INVENTION
The present invention relates to a medicament to be used to prevent and/or treat synucleinopathies.
BACKGROUND OF THE INVENTION
Synucleinopathies are a diverse group of neurodegenerative disorders that share a common pathologic characteristic: in neuropathologic examinations characteristic lesions can be detected containing abnormal aggregates of alpha-synuclein (alpha-syn, a-syn) protein in selected populations of neurons and glia cells.
Alpha-syn (initially identified as PARK1 and PARK4) is a 140 amino acid protein widely expressed in the neocortex, hippocampus, dentate gyrus, olfactory bulb, striatum, thalamus and cerebellum. Alpha-Syn is also highly expressed in hematopoietic cells including B—, T—, and NK cells as well as monocytes and platelets. The exact role in these cells is not known but it has been implicated in the differentiation of megakaryocytes (platelet precursors).
The most common synucleinopathies include but are not limited to Lewy body disorders (LBDs) like Parkinson's disease (PD), Parkinson's disease with dementia (PDD) and dementia with Lewy bodies (DLB), as well as Multiple System Atrophy (MSA) or Neurodegeneration with Brain Iron Accumulation type I (NBIA Type I). The current treatment options for these diseases include symptomatic medications such as L-dopa, anticholinergic drugs as well as inhibitors of monoamine oxidase. However, all treatment opportunities currently present only lead to symptomatic alleviation but do not induce a long lasting, disease modifying effect in patients.
Lewy body disorders (LBD) are progressive neurodegenerative disorders characterized by tremor, rigidity, bradykinesia and by loss of dopaminergic neurons in the brain. In the case of DLB and PDD signs also include cognitive impairment. Up to 2% of the population above 60 years of age in western countries develop the typical signs of PD/LBD. Currently only symptomatic treatment is available. Unfortunately, these therapies only provide temporary relief from early symptoms and do not halt disease progression. The pathogenesis of PD/LBD is still incompletely understood, but it appears that genetic susceptibility and environmental factors are involved in the development of the disease. Despite all genetic advances, PD/LBD is primarily a sporadic disorder with no known cause (also called idiopathic PD/LBD).
Patients suffering from this disease develop characteristic ubiquitinated intracellular inclusions called Lewy bodies (LBs) in the cortical and subcortical areas of the brain. Especially regions with high content of dopaminergic neurons or neuronal projections show this typical pathologic feature. Recently, several studies could show that the synaptic protein alpha-syn plays a central role in LBD pathogenesis. In LBD, alpha-syn accumulates in LBs throughout affected brain areas. Additionally, it could be demonstrated that single point mutations as well as duplications or multiplications in the alpha-syn gene are associated with rare familial forms of parkinsonism. Importantly, based on results from overexpression studies in transgenic (tg) mice as well as in Drosophila melanogaster its key role in the pathogenesis of PD/LBD is underscored as these animal models mimic several characteristics of PD.
Another very important synucleinopathy is Multiple System Atrophy (MSA). MSA is a sporadic neurodegenerative disorder that is characterised by symptoms of L-DOPA-resistant parkinsonism, cerebellar ataxia, and dysautonomia. Patients suffer from multi-system neuronal loss affecting various brain areas including striatum, substantia nigra, cerebellum, pons, as well as the inferior olives and the spinal cord. MSA is characterized by al-pha syn-positive glial cytoplasmic (GCI) and rare neuronal inclusions throughout the central nervous system. These inclusions are associated with striatonigral degeneration, olivopontocerebellar atrophy, and involvement of autonomic nuclei in medulla and spinal cord. The importance of GCIs for the pathogenesis of MSA is generally acknowledged and underscored by recent analysis of transgenic mouse models analysing the effect of alpha-syn overexpression in oligodendroglia. In tg mice overexpressing human alpha-syn both GCI-like aggregates and biochemical markers of MSA were observed.
Although the exact mechanisms by which accumulation of al-pha-syn leads to the typical features of neurodegeneration in synucleopathies are not fully understood, recent studies imply that abnormal formation and accumulation of alpha-syn is involved in the degenerative processes underlying synucleinopathy. Recently, different forms of alpha-syn have been identified in LBs. Beside the full length form of the protein, different forms of modified alpha-syn have been identified including phosphorylated, nitrated, and mono-, di-, or tri-ubiquitinated alpha-syn. In addition, C-terminally truncated forms of the protein, like alpha-syn 1-119, alpha-syn 1-122 and alpha-syn 1-123, have been detected in brain tissue from both transgenic mice and PD cases. It is currently believed that up to 15% of the alpha-syn detected in LBs and lewy neurites is truncated. Previous in vitro studies using truncated alpha-syn could demonstrate that alpha-syn lacking the C-terminal 20-30 amino acids was showing an increased tendency to aggregate and to form filaments found in Lewy-neurites and LBs. These truncated versions could thus act in a similar way as truncated and modified forms of amyloid beta (Aβ) in Alzheimer's disease (AD). These truncated and modified forms of Aβ are thought to act as seed molecules for plaque deposition and show a higher aggregation propensity as well as high neurotoxicity and synaptotoxicity in vivo and in vitro.
Thus full length alpha-syn as well as truncated and/or modified forms of alpha-syn, which are showing potential seeding effects, are then believed to accumulate leading to oligomer-formation. Based on recent studies it is believed that such oligomer-formation for example in the synaptic terminals and axons plays an important role for PD/LBD development and could thus be enhanced by the presence of truncated forms of alpha-syn. Hence, reduction of alpha-syn deposition and oligomerisation should be beneficial in the treatment of synucleopathies, especially of idiopathic LBD/PD and MSA and could present the first strategy for treatment of these neurodegenerative diseases in addition to the mere alleviation of symptoms resulting from current treatment strategies like L-DOPA application.
In Iwatsubo T. (Neuropathology 27 (5) (2007): 474-478) the correlation of alpha-synuclein depositions as well as its phosphorylation with a pathogenesis of alpha-synucleopathies is examined. The author of this publication found that serine 129 of alpha-synuclein deposited in synucleopathy lesions is extensively phosphorylated. US 2007/213253 relates to mutant human alpha-synuclein as well as peptides derived therefrom which may be used for inhibiting the aggregation of the wild-type human alpha-synuclein. In the WO 2004/041067 means and methods for preventing or treating diseases associated with alpha-synuclein aggregation are disclosed which comprise the use of alpha-synuclein fragments. In the US 2003/166558 peptides are described which can be used to induce immune response to protein deposits. US 2005/198694 relates to alpha-synuclein fragments comprising at least 100 amino acids and having a C-terminal deletion of 1 to 23 amino acids.
Liang et al. (J. Neurochem. 99 (2006): 470-482) studied the regulation of alpha-synuclein in rats. They observed that in alcohol preferring rats the expression rate of alpha-synuclein is increased compared to alcohol-non preferring rats.
In Hamilton B A (Genomics 83 (2004): 739-742) the distribution of alpha-synuclein 53Thr and 53Ala in primates is examined.
In US 2005/0037013 immunogenic alpha-synuclein fragments are disclosed which are able to induce an immune response against a specific epitope within residues 70-140 of alpha-synuclein.
WO 2006/045037 relates to C-terminal truncated alpha-synuclein molecules which can be used to screen for agents which have a pharmacological activity useful for treating a Lewy Body Disease.
Although experimental therapies utilizing neurotrophic factors and grafting of dopaminergic cells have yielded promising results, alternative approaches designed to reduce the neuronal accumulation of alpha-syn are required. There is compelling evidence accumulating that alpha-syn aggregates might be targeted by immunotherapy. Indeed, recently a potential for the treatment of synucleopathies has been shown. Tg mice overexpressing human alpha-syn were vaccinated with human alpha-syn protein. In mice that produced high relative affinity antibodies upon vaccination, there was decreased accumulation of aggregated alpha-syn in neuronal cell bodies and synapses which was associated with reduced neurodegeneration. Furthermore, antibodies produced by immunized animals also detected abnormal aggregated forms of alpha-syn associated with the neuronal membrane and promoted the degradation of these aggregates, probably via lysosomal pathways. Similar effects were observed using passive immunotherapy with an exogenously applied alpha-syn-specific antibody. These results suggest that vaccination is effective in reducing neuronal accumulation of alpha-syn aggregates and that further development of this approach might elicit beneficial effects in the treatment of LBD and synucleinopathies.
BRIEF SUMMARY OF THE INVENTION
It is an object of the present invention to provide a medicament to prevent and treat synucleinopathies on the basis of a vaccine.



BRIEF DESCRIPTION OF THE DRAWINGS
The present invention is further illustrated in the following figures and examples, however, without being restricted thereto.
FIG.1 shows the sequence of full length alpha-synuclein (140aa; swiss prot entry: P37840) and the sequence used to create a monoclonal antibody for detection of full length alpha-synuclein as well as C-terminally truncated and modified versions thereof. The peptide at position 100-109 used for generation of the monoclonal antibody is underlined. The peptide (p4453) was coupled to a C at C-terminal position.
FIG.2 shows detection of alpha-synuclein by ELISA using the generated monoclonal antibody specific for human alpha-synuclein at position 100-109. The monoclonal antibody 12-9-9 was generated and tested for its specificity to synucleins in ELISA. Alpha-synuclein (p4446) and p4453 the human epitope are detected. The negative control protein p4447 (beta-synuclein) is not detected.
FIG. 3 shows the definition of the minimal epitope of the monoclonal antibody 12-9-9 by ELISA.
The peptides p4446 (alpha-synuclein), p4453(human epitope used to create the antibody tested) are detected by the antibody. The original epitope p4453 was N- or C-terminally truncated and used for ELISA to define the minimal epitope required for specific binding. The peptides p5399 and p5403 lost binding to the monoclonal antibody 12-9-9. Thus the minimal sequence needed for binding of 12-9-9 is predicted as KNEEGAP located at position 102-108 of alpha-synuclein, while truncation of one of the flanking amino acids abolished the binding. Data are presented in a linear scale.
FIG. 4 shows detection of epitopes and mimotopes by ELISA using a monoclonal specific for human alpha-synuclein at position 100-109. Alpha-Synuclein as well as the peptides p5436 (human minimal epitope) and the mimotopes p5439 are detected similarly by the monoclonal antibody 12-9-9. The mimotope p5440 is not detected, while mimotope p5444 is detected much weaker than the human epitope by the monoclonal antibody 12-9-9.
FIG. 5 shows the induction of immune response against alpha-synuclein after peptide immunization. Sera of immunized mice (p5436 to p5590) show titers against alpha-synuclein after 3 vaccinations. Sera of immunized mice (p5463 to p5466) do not detect alpha synuclein (Titers measured in ELISA are around or below 1:50 half-max). Class of immunogenicity was defined as follows: Class 2: peptides inducing an immune response with OD halfmax higher than 1:1000. Class 1: peptides inducing an immune response with OD halfmax between 1000 and 51. Class 0: peptides inducing no or an very low immune resonse with OD halfmax around 50 or lower.



DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to the provision of at least one peptide or polypeptide comprising the amino acid sequence

(X1)nX2X3X4X5GX6P(X7)m(SEQ ID NO: 78)  (Formula I),

wherein

    
    
        X1 is any amino acid residue,
        X2 is an amino acid residue selected from the group consisting of lysine (K), arginine (R), alanine (A) and histidine (H),
        X3 is an amino acid residue selected from the group consisting of asparagine (N), glutamine (Q), serine (S), glycine (G) and alanine (A), preferably asparagine (N), serine (S), glycine (G) and alanine (A),
        X4 is an amino acid residue selected from the group consisting of glutamic acid (E), aspartic acid (D) and alanine (A),
        X5 is an amino acid residue selected from the group consisting of glutamic acid (E) and aspartic acid (D),
        X6 is an amino acid residue selected from the group consisting of alanine (A) and tyrosine (Y),
        X7 is any amino acid residue,
        n and m, independently, are 0 or an integer of more than 0,
    
    


and wherein the amino acid sequence according to Formula I is not identical with, or does not comprise the 7-mer polypeptide fragment of alpha-synuclein having the amino acid sequence KNEEGAP (SEQ ID NO: 139),
said at least one peptide or polypeptide having a binding capacity to an antibody which is specific for an epitope of alpha-synuclein comprising the amino acid sequence KNEEGAP (SEQ ID NO: 139),
for use in preventing and/or treating synucleinopathies.
These peptides or polypeptides according to the present invention can be provided in compositions suitable for the intended use for preventing and/or treating synucleinopathies, especially in pharmaceutical compositions, preferably combined with a pharmaceutically acceptable carrier. Such pharmaceutical compositions can be administered to a patient in need thereof in an effective amount to achieve the preventive and/or therapeutic effect.
The peptides and polypeptide according to the present invention are able to induce the in vivo formation of antibodies directed (binding) to alpha-synuclein and fragments thereof, in particular to fragments of alpha-synuclein comprising the amino acid sequence KNEEGAP (SEQ ID NO: 139). Antibodies directed (binding) to said peptides and polypeptides, however, show no or substantially no immune reactivity to beta-synuclein (beta-syn, b-syn). Therefore, unlike the original alpha-synuclein or fragment(s) thereof, the peptides and polypeptides according to the present invention provide a specificity towards the disease related agent and avoid cross reactivity with disease unrelated synucleins. This strongly suggests significant superiority regarding efficacy and safety, the latter in particular because of the neuroprotective characteristics that have been described for beta-synuclein (Hashimoto M. et al., J Biol. Chem. 2004 May 28; 279(22):23622-9. Hashimoto M, Neuron. 2001 Oct. 25; 32(2):213-23).
The alpha-synuclein specific antibodies induced by the administration of the compounds of the present invention might not only bind to monomeric forms of alpha-synuclein but also to multimeric forms. This allows to reduce the amount of oligomers of alpha-synuclein in the body of an individual to be treated. The reduction of alpha-synuclein is particularly beneficial in the treatment of synucleopathies.
The amino acid sequence (X1)nX2X3X4X5GX6P(X7)m (SEQ ID NO: 78)is considered to be a mimotope of the epitope of alpha-synuclein comprising the amino acid sequence KNEEGAP (SEQ ID NO: 139). According to the present invention the term “mimotope” refers to a molecule which has a conformation that has a topology equivalent to the epitope of which it is a mimic. The mimotope binds to the same antigen-binding region of an antibody which binds immunospecifically to a desired antigen. The mimotope will elicit an immunological response in a host that is reactive to the antigen to which it is a mimic. The mimotope may also act as a competitor for the epitope of which it is a mimic in in vitro inhibition assays (e.g. ELISA inhibition assays) which involve the epitope and an antibody binding to said epitope. However, a mimotope of the present invention may not necessarily prevent or compete with the binding of the epitope of which it is a mimic in an in vitro inhibition assay although it is capable to induce a specific immune response when administered to a mammal.
As used herein, the term “epitope” refers to an immunogenic region of an antigen which is recognized by a particular anti-body molecule. In general, an antigen will possess one or more epitopes, each capable of binding an antibody that recognizes the particular epitope.
The mimotopes of the present invention can be synthetically produced by chemical synthesis methods which are well known in the art, either as an isolated peptide or as a part of another peptide or polypeptide. Alternatively, the peptide mimotope can be produced in a microorganism which produces the peptide mimotope which is then isolated and if desired, further purified. The peptide mimotope can be produced in microorganisms such as bacteria, yeast or fungi, in eukaryote cells such as a mammalian or an insect cell, or in a recombinant virus vector such as adenovirus, poxvirus, herpesvirus, Simliki forest virus, baculovirus, bacteriophage, sindbis virus or sendai virus. Suitable bacteria for producing the peptide mimotope include E. coli, B. subtilis or any other bacterium that is capable of expressing peptides such as the peptide mimotope. Suitable yeast types for expressing the peptide mimotope include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida, Pichia pastoris or any other yeast capable of expressing peptides. Corresponding methods are well known in the art. Also methods for isolating and purifying recombinantly produced peptides are well known in the art and include e.g. gel filtration, affinity chromatography, ion exchange chromatography etc.
To facilitate isolation of the peptide mimotope, a fusion polypeptide may be made wherein the peptide mimotope is translationally fused (covalently linked) to a heterologous polypeptide which enables isolation by affinity chromatography. Typical heterologous polypeptides are His-Tag (e.g. His6; 6 histidine residues) (SEQ ID NO: 140)), GST-Tag (Glutathione-S-transferase) etc. The fusion polypeptide facilitates not only the purification of the mimotopes but can also prevent the mimotope polypeptide from being degraded during purification. If it is desired to remove the heterologous polypeptide after purification the fusion polypeptide may comprise a cleavage site at the junction between the peptide mimotope and the heterologous polypeptide. The cleavage site consists of an amino acid sequence that is cleaved with an enzyme specific for the amino acid sequence at the site (e.g. proteases).
According to a preferred embodiment of the present invention, X2 is an amino acid residue selected from the group consisting of lysine (K) and arginine (R) and/or X6 is alanine (A).
According to a particularly preferred embodiment of the present invention, the peptide or polypeptide comprises an amino acid sequence selected from the group consisting of (X1)nKNDEGAP(X7)m (SEQ ID NO: 87), (X1)nANEEGAP(X7)m (SEQ ID NO: 79), (X1)nKAEEGAP(X7)m (SEQ ID NO: 80), (X1)nKNAEGAP(X7)m (SEQ ID NO: 81), (X1)nRNEEGAP(X7)m (SEQ ID NO: 85), (X1)nHNEEGAP(X7)m (SEQ ID NO: 86), (X1)nKNEDGAP(X7)m (SEQ ID NO: 88), (X1)nKQEEGAP(X7)m (SEQ ID NO: 89), (X1)nKSEEGAP(X7)m (SEQ ID NO: 90), (X1)nKNDDGAP(X7)m (SEQ ID NO: 91), (X1)nRNDEGAP(X7)m (SEQ ID NO: 115), (X1)nRNEDGAP(X7)m (SEQ ID NO: 116), (X1)nRQEEGAP(X7)m (SEQ ID NO: 117), (X1)nRSEEGAP(X7)m (SEQ ID NO: 118), (X1)nANDEGAP(X7)m (SEQ ID NO: 119), (X1)nANEDGAP(X7)m (SEQ ID NO: 120), (X1)nHSEEGAP(X7)m (SEQ ID NO: 121), (X1)nASEEGAP(X7)m (SEQ ID NO: 122), (X1)nHNEDGAP(X7)m (SEQ ID NO: 123), (X1)nHNDEGAP(X7)m (SEQ ID NO: 124), (X1)nRNAEGAP(X7)m (SEQ ID NO: 125), (X1)nHNAEGAP(X7)m (SEQ ID NO: 126), (X1)nKSAEGAP(X7)m (SEQ ID NO: 127), (X1)nKSDEGAP(X7)m (SEQ ID NO: 128), (X1)nKSEDGAP(X7)m (SEQ ID NO: 129), (X1)nRQDEGAP(X7)m (SEQ ID NO: 141), (X1)nRQEDGAP(X7)m (SEQ ID NO: 131), (X1)nHSAEGAP(X7)m (SEQ ID NO: 132), (X1)nRSAEGAP(X7)m (SEQ ID NO: 133), (X1)nRSDEGAP(X7)m (SEQ ID NO: 134), (X1)nRSEDGAP(X7)m (SEQ ID NO: 135), (X1)nHSDEGAP(X7)m (SEQ ID NO: 136), (X1)nHSEDGAP(X7)m (SEQ ID NO: 137), (X1)nRQDDGAP(X7)m (SEQ ID NO: 138), preferably (X1)nKNDEGAP(X2)m (SEQ ID NO: 87), (X1)nRNEEGAP(X2)m (SEQ ID NO: 85), (X1)nRNDEGAP(X2)m (SEQ ID NO: 115), (X1)nKNAEGAP(X2)m (SEQ ID NO: 81), (X1)nKSDEGAP(X2)m (SEQ ID NO: 128), (X1)nRNAEGAP(X2)m (SEQ ID NO: 125)or (X1)nRSEEGAP(X2)m (SEQ ID NO: 118).
It turned out that not only peptides and polypeptides according to formula I can be used in the treatment and prevention of synucleinopathies, but also other peptides and polypeptides. Therefore, another aspect of the present invention relates to at least one peptide or polypeptide comprising an amino acid sequence selected from the group consisting of (X1)nKNEAGAP(X7)m (SEQ ID NO: 82), (X1)nKNEEAAP(X7)m (SEQ ID NO: 83), (X1)nKNEEGAA(X7)m (SEQ ID NO: 84), (X1)nKPSFKNE(X7)m (SEQ ID NO: 92), (X1)nQPSFAME(X7)m (SEQ ID NO: 93), (X1)nSPSFKQE(X7)m (SEQ ID NO: 94), (X1)nTPSWKGE(X7)m (SEQ ID NO: 95), (X1)nDPSFALE(X7)m (SEQ ID NO: 96), (X1)nLPSFRLE(X7)m (SEQ ID NO: 97), (X1)nEPNSRMD(X7)m (SEQ ID NO: 98), (X1)nQPSSKLD(X7)m (SEQ ID NO: 99), (X1)nHIHQSKFFDAPP(X7)m (SEQ ID NO: 100), (X1)nQASFAME(X7)m (SEQ ID NO: 101), (X1)nTASWKGE(X7)m (SEQ ID NO: 102), (X1)nQASSKLD(X7)m (SEQ ID NO: 103), (X1)nQPAFAME(X7)m (SEQ ID NO: 104), (X1)nTPAWKGE(X7)m (SEQ ID NO: 105), (X1)nQPASKLD(X7)m (SEQ ID NO: 106), (X1)nQPSFAMA(X7)m (SEQ ID NO: 107), (X1)nTPSWKGA(X7)m (SEQ ID NO: 108), (X1)nQPSSKLA(X7)m (SEQ ID NO: 109), (X1)nAPSWKGE(X7)m (SEQ ID NO: 110), (X1)nTPSAKGE(X7)m (SEQ ID NO: 111), (X1)nTPSWAGE(X7)m (SEQ ID NO: 112), (X1)nTPSWKAE(X7)m (SEQ ID NO: 113), (X1)nTPSWKGE(X7)m (SEQ ID NO: 114), especially an amino acid sequence selected from the group consisting of (X1)nQASFAME(X7)m (SEQ ID NO: 101), (X1)nTASWKGE(X7)m (SEQ ID NO: 102), (X1)nQASSKLD(X7)m (SEQ ID NO: 103), (X1)nTPAWKGE(X7)m (SEQ ID NO: 105), (X1)nTPSWAGE(X7)m (SEQ ID NO: 112), (X1)nTPSWKGE(X7)m (SEQ ID NO: 95),
wherein
X1 is any amino acid residue,
X7 is any amino acid residue,
n and m, independently, are 0 or an integer of more than 0,
said at least one peptide or polypeptide having a binding capacity to an antibody which is specific for an epitope of alpha-synuclein comprising the amino acid sequence KNEEGAP (SEQ ID NO: 139),
for use in preventing and/or treating synucleinopathies, especially for the production of a medicament therefor.
The peptides and polypeptides of the present invention may also be modified at or nearby their N- and/or C-termini so that at said positions a cysteine residue is bound thereto. In a preferred embodiment terminally positioned (located at the N- and C-termini of the peptide) cysteine residues are used to cross-link said molecules with carrier molecules such as KLH or to cyclize the peptides through a disulfide bond. Therefore, n and/or m are preferably 1 and X1 and/or X7 are preferably cysteine (C).
The mimotopes of the present invention may also be used in various assays and kits, in particular in immunological assays and kits. Therefore, it is particularly preferred that the peptides and polypeptides of the present invention may be part of another peptide or polypeptide, particularly an enzyme which is used as a reporter in immunological assays. Such reporter enzymes include e.g. alkaline phosphatase or horseradish peroxidase.
The alpha-synuclein mimotopes according to the present invention preferably are antigenic polypeptides which in their amino acid sequence vary from the amino acid sequence of alpha-synuclein or of fragments of alpha-synuclein. In this respect, the inventive mimotopes may not only comprise amino acid substitutions of one or more naturally occurring amino acid residues but also of one or more non-natural amino acids (i.e. not from the 20 “classical” amino acids) or they may be completely assembled of such non-natural amino acids. Moreover, the inventive antigens which induce anti-alpha-synuclein antibodies may be assembled of D- or L-amino acids or of combinations of DL-amino acids and, optionally, they may have been changed by further modifications, ring closures or derivatizations. Suitable antialpha-synuclein-antibody-inducing antigens may be provided from commercially available peptide libraries. Preferably, these peptides are at least 7 amino acids, and preferred lengths may be up to 16, preferably up to 14 or 20 amino acids residues (e.g. 7 or 8 to 20, 7 or 8 to 16 etc.). Thus, the peptide or polypeptide of the present invention comprises 7 to 30, preferably 7 to 20, more preferably 7 to 16, most preferably 8, amino acid residues. According to the invention, however, also longer peptides may very well be employed as anti-alpha-synuclein-antibody-inducing antigens. Furthermore the mimotopes of the present invention may also be part of a polypeptide and consequently comprising at their N- and/or C-terminus at least one further amino acid residue.
For preparing alpha-synuclein mimotopes (i.e. anti-alpha-synuclein-antibody-inducing antigens), of course also phage libraries, peptide libraries are suitable, for instance produced by means of combinatorial chemistry or obtained by means of high throughput screening techniques for the most varying structures (Display: A Laboratory Manual by Carlos F. Barbas (Editor), et al.; Willats W G Phage display: practicalities and prospects. Plant Mol. Biol. 2002 December; 50(6):837-54).
Furthermore, according to the invention also anti-alpha-synuclein-antibody-inducing antigens based on nucleic acids (“aptamers”) may be employed, and these, too, may be found with the most varying (oligonucleotide) libraries (e.g. with 2-180 nucleic acid residues) (e.g. Burgstaller et al., Curr. Opin. Drug Discov. Dev. 5(5) (2002), 690-700; Famulok et al., Acc. Chem. Res. 33 (2000), 591-599; Mayer et al., PNAS 98 (2001), 4961-4965, etc.). In anti-alpha-synuclein-antibody-inducing antigens based on nucleic acids, the nucleic acid backbone can be provided e.g. by the natural phosphor-diester compounds, or also by phosphorotioates or combinations or chemical variations (e.g. as PNA), wherein as bases, according to the invention primarily U, T, A, C, G, H and mC can be employed. The 2′-residues of the nucleotides which can be used according to the present invention preferably are H, OH, F, Cl, NH2, O-methyl, O-ethyl, O-propyl or O-butyl, wherein the nucleic acids may also be differently modified, i.e. for instance with protective groups, as they are commonly employed in oligonucleotide synthesis. Thus, aptamer-based anti-alpha-synuclein-antibody-inducing antigens are also preferred anti-alpha-synuclein-antibody-inducing antigens within the scope of the present invention.
According to the present invention the term “synucleinopathy” includes all neurodegenerative disorders characterized by pathological synuclein aggregations. Several neurodegenerative disorders including Parkinson's Disease (PD), Lewy Body Disease (LBD), Diffuse Lewy Body Disease (DLBD), Dementia with Lewy Bodies (DLB), Parkinsonism with Dementia (PDD), Multiple System Atrophy (MSA) and Neurodegeneration with Brain Iron Accumulation type I (NBIA Type I) are collectively grouped as synucleinopathies.
The peptides and polypeptides according to the present invention may be employed not only for treating synucleinopathies but also to prevent said diseases in individuals being at risk of developing a synucleinopathy (e.g. predisposed, for example genetically predisposed, to developing a synucleinopathy).
The abbreviations for the amino acid residues disclosed in the present invention follow the IUPAC recommendations:















Amino Acid
3-Letter Code
1-Letter Code









Alanine
Ala
A



Arginine
Arg
R



Asparagine
Asn
N



Aspartic
Asp
D



Cysteine
Cys
C



Glutamic
Glu
E



Glutamine
Gln
Q



Glycine
Gly
G



Histidine
His
H



Isoleucine
Ile
I



Leucine
Leu
L



Lysine
Lys
K



Methionine
Met
M



Phenylalanine
Phe
F



Proline
Pro
P



Serine
Ser
S



Threonine
Thr
T



Tryptophan
Trp
W



Tyrosine
Tyr
Y



Valine
Val
V










The peptides and polypeptides of the present invention may also be part of a polypeptide comprising 7 to 30 amino acid residues. Consequently n and m may independently be an integer selected from the group of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20 and 25.
The at least one peptide or polypeptide according to the present invention may consist of the amino acid sequence (X1)nX2X3X4X5GX6P(X7)m (SEQ ID NO: 142), wherein n and m are independently 0 or 1 or being part of a polypeptide which comprises at least 7 amino acid residues, preferably at least 10 amino acid residues, more preferably at least 15 amino acid residue, and/or a maximum of 50 amino acid residues, preferably a maximum of 30 amino acid residues, more preferably of 16 amino acid residues.
Surprisingly, it turned out that the compounds according to the present invention comprising or consisting of the amino acid sequences listed above are particularly suited to be used for the manufacture of a medicament to be used to treat or prevent synucleinopathies. These peptides (mimotopes) are able to induce the in vivo formation of antibodies directed to the original epitope of human alpha-synuclein comprising the amino acid sequence KNEEGAP (SEQ ID NO: 139) and human alpha-synuclein protein itself. Said peptides (mimotopes) are, however, not able to induce immune reactivity against human beta-synuclein protein. The peptide induced antibodies are responsible for the removal of alpha-synuclein (which is involved in the formation of alpha-synuclein aggregates, Lewy bodies) and/or for the dissolution of alpha-synuclein aggregates (Lewy bodies) in an individual.
The peptides and polypeptides according to the present invention may be used for the preparation of a medicament, in particular a vaccine, which can be used to treat alpha-synucleinopathy, whereby the medicament is particularly suited to treat synucleinopathy selected from the group consisting of Parkinson's Disease (PD), Lewy Body Disease (LBD), Diffuse Lewy Body Disease (DLBD), Dementia with Lewy Bodies (DLB), Parkinsonism with Dementia (PDD), Multiple System Atrophy (MSA) and Neurodegeneration with Brain Iron Accumulation type I (NBIA Type I).
According to a preferred embodiment of the present invention the at least one peptide or polypeptide is coupled to a pharmaceutically acceptable carrier, preferably KLH (Keyhole Limpet Hemocyanin), tetanus toxoid, albumin-binding protein, bovine serum albumin, a dendrimer (MAP; Biol. Chem. 358: 581), peptide linkers (or flanking regions) as well as the adjuvant substances described in Singh at al., Nat. Biotech. 17 (1999), 1075-1081 (in particular those in Table 1 of that document), and O'Hagan at al., Nature Reviews, Drug Discovery 2 (9) (2003), 727-735 (in particular the endogenous immuno-potentiating compounds and delivery systems described therein), and others or mixtures thereof. The conjugation chemistry (e.g. via heterobifunctional compounds such as GMBS and of course also others as described in “Bioconjugate Techniques”, Greg T. Hermanson) in this context can be selected from reactions known to the skilled man in the art. Moreover, the vaccine composition may be formulated with an adjuvant, preferably a low soluble aluminium composition, in particular aluminium hydroxide. Of course, also adjuvants like MF59 aluminium phosphate, calcium phosphate, cytokines (e.g., IL-2, IL-12, GM-CSF), saponins (e.g., QS21), MDP derivatives, CpG oligos, IC31, LPS, MPL, polyphosphazenes, emulsions (e.g., Freund's, SAF), liposomes, virosomes, iscoms, cochleates, PLG microparticles, poloxamer particles, virus-like particles, heat-labile enterotoxin (LT), cholera toxin (CT), mutant toxins (e.g., LTK63 and LTR72), microparticles and/or polymerized liposomes may be used.
The peptide or polypeptide of the present invention is preferably bound to the carrier or adjuvant via a linker, which is selected from the group consisting of NHS-poly (ethylene oxide) (PEO) (e.g. NHS-PEO4-maleimide).
A vaccine which comprises the present compound (mimotope) and the pharmaceutically acceptable carrier may be administered by any suitable mode of application, e.g. i.d., i.p., i.m., intranasally, orally, subcutaneously, etc. and in any suitable delivery device (O'Hagan at al., Nature Reviews, Drug Discovery 2 (9), (2003), 727-735). The compound of the present invention is preferably formulated for subcutaneous, intradermal or intramuscular administration (see e.g. “Handbook of Pharmaceutical Manufacturing Formulations”, Sarfaraz Niazi, CRC Press Inc, 2004).
Typically, the vaccine contains the compound according to the invention in an amount of from 0.1 ng to 10 mg, preferably 10 ng to 1 mg, in particular 100 ng to 100 μg, or, alternatively, e.g. 100 fmol to 10 μmol, preferably 10 pmol to 1 μmol, in particular 100 pmol to 100 nmol. Typically, the vaccine may also contain auxiliary substances, e.g. buffers, stabilizers etc. Preferably, such auxiliary substances, e.g. a pharmaceutically acceptable excipient, such as water, buffer and/or stabilisers, are contained in an amount of 1 to 99% (weight), more preferred 5 to 80% (weight), especially 10 to 70% (weight). Possible administration regimes include a weekly, biweekly, four-weekly (monthly) or bimonthly treatment for about 1 to 12 months; however, also 2 to 5, especially 3 to 4, initial vaccine administrations (in one or two months), followed by boaster vaccinations 6 to 12 months thereafter or even years thereafter are preferred—besides other regimes already suggested for other vaccines. Another aspect of the present invention relates to a peptide having an amino acid sequence selected from the group consisting of (X1)nKNDEGAP(X7)m (SEQ ID NO: 143), (X1)nANEEGAP(X7)m (SEQ ID NO: 144), (X1)nKAEEGAP(X7)m (SEQ ID NO: 145), (X1)nKNAEGAP(X7)m (SEQ ID NO: 146), (X1)nKNEAGAP(X7)m (SEQ ID NO: 147), (X1)nKNEEAAP(X7)m (SEQ ID NO: 148), (X1)nKNEEGAA(X7)m (SEQ ID NO: 149), (X1)nRNEEGAP(X7)m (SEQ ID NO: 150), (X1)nHNEEGAP(X7)m (SEQ ID NO: 151), (X1)nKNEDGAP(X7)m (SEQ ID NO: 152), (X1)nKQEEGAP(X7)m (SEQ ID NO: 153), (X1)nKSEEGAP(X7)m (SEQ ID NO: 154), (X1)nKNDDGAP(X7)m (SEQ ID NO: 155), (X1)nKPSFKNE (X7)m (SEQ ID NO: 156),(X1)nQPSFAME(X7)m (SEQ ID NO: 157), (X1)nSPSFKQE(X7)m (SEQ ID NO: 158), (X1)nTPSWKGE(X7)m (SEQ ID NO: 159), (X1)nQPSFALE(X7)m (SEQ ID NO: 160), (X1)nLPSFRLE (X7)m (SEQ ID NO: 161), (X1)nEPNSRMD(X7)m (SEQ ID NO: 162),(X1)nQPSSKLD(X7)m (SEQ ID NO: 163), (X1)nHIHQSKFFDAPP(X7)m (SEQ ID NO: 164), (X1)nQASFAME(X7)m (SEQ ID NO: 165), (X1)nTASWKGE(X7)m (SEQ ID NO: 166), (X1)nQASSKLD(X7)m (SEQ ID NO: 167), (X1)nQPAFAME(X7)m (SEQ ID NO: 168), (X1)nTPAWKGE(X7)m (SEQ ID NO: 169), (X1)nQPASKLD(X7)m (SEQ ID NO: 170), (X1)nQPSFAMA(X7)m (SEQ ID NO: 171), (X1)nTPSWKGA(X7)m (SEQ ID NO: 172), (X1)nQPSSKLA(X7)m (SEQ ID NO: 173), (X1)nAPSWKGE(X7)m (SEQ ID NO: 174), (X1)nTPSAKGE(X7)m (SEQ ID NO: 175), (X1)nTPSWAGE(X7)m (SEQ ID NO: 176), (X1)nTPSWKAE(X7)m (SEQ ID NO: 177), (X1)nTPSWKGE(X7)m (SEQ ID NO: 178), (X1)nRNDEGAP(X7)m (SEQ ID NO: 179), (X1)nRNEDGAP(X7)m (SEQ ID NO: 180), (X1)nRQEEGAP(X7)m (SEQ ID NO: 181), (X1)nRSEEGAP(X7)m (SEQ ID NO: 182), (X1)nANDEGAP(X7)m (SEQ ID NO: 183), (X1)nANEDGAP(X7)m (SEQ ID NO: 184), (X1)nKSEEGAP(X7)m (SEQ ID NO: 185), (X1)nASEEGAP(X7)m (SEQ ID NO: 186), (X1)nHNEDGAP(X7)m (SEQ ID NO: 187), (X1)nHNDEGAP(X7)m (SEQ ID NO: 188), (X1)nRNAEGAP(X7)m (SEQ ID NO: 189), (X1)nHNAEGAP(X7)m (SEQ ID NO: 190), (X1)nKSAEGAP(X7)m (SEQ ID NO: 191), (X1)nKSDEGAP(X7)m (SEQ ID NO: 192), (X1)nKSEDGAP(X7)m (SEQ ID NO: 193), (X1)nRQDEGAP(X7)m (SEQ ID NO: 194), (X1)nRQEDGAP(X7)m (SEQ ID NO: 195), (X1)nHSAEGAP(X7)m (SEQ ID NO: 196), (X1)nRSAEGAP(X7)m (SEQ ID NO: 197), (X1)nRSDEGAP(X7)m (SEQ ID NO: 198), (X1)nRSEDGAP(X7)m (SEQ ID NO: 199), (X1)nHSDEGAP(X7)m (SEQ ID NO: 200), (X1)nHSEDGAP(X7)m (SEQ ID NO: 201) and (X1)nRQDDGAP(X7)m (SEQ ID NO: 202), especially an amino acid sequence selected from the group consisting of (X1)nKNDEGAP(X7)m (SEQ ID NO: 143), (X1)nANEEGAP(X7)m (SEQ ID NO: 144), (X1)nKAEEGAP(X7)m (SEQ ID NO: 145), (X1)nKNAEGAP(X7)m (SEQ ID NO: 146), (X1)nRNEEGAP(X7)m (SEQ ID NO: 150), (X1)nHNEEGAP(X7)m (SEQ ID NO: 151), (X1)nKNEDGAP(X7)m (SEQ ID NO: 152), (X1)nKQEEGAP(X7)m (SEQ ID NO: 153), (X1)nKSEEGAP(X7)m (SEQ ID NO: 154), (X1)nKNDDGAP(X7)m (SEQ ID NO: 155), (X1)nQASFAME(X7)m (SEQ ID NO: 165), (X1)nTASWKGE(X7)m (SEQ ID NO: 166), (X1)nQASSKLD(X7)m (SEQ ID NO: 167), (X1)nTPAWKGE(X7)m (SEQ ID NO: 169), (X1)nTPSWAGE(X7)m (SEQ ID NO: 176), (X1)nTPSWKGE(X7)m (SEQ ID NO: 178), (X1)nRNDEGAP(X7)m (SEQ ID NO: 179), (X1)nRNEDGAP(X7)m (SEQ ID NO: 180), (X1)nRQEEGAP(X7)m (SEQ ID NO: 181), (X1)nRSEEGAP(X7)m (SEQ ID NO: 182), (X1)nANDEGAP(X7)m (SEQ ID NO: 183), (X1)nANEDGAP(X7)m (SEQ ID NO: 184), (X1)nHSEEGAP(X7)m (SEQ ID NO: 185), (X1)nASEEGAP(X7)m (SEQ ID NO: 186), (X1)nHNEDGAP(X7)m (SEQ ID NO: 187), (X1)nHNDEGAP(X7)m (SEQ ID NO: 188), (X1)nRNAEGAP(X7)m (SEQ ID NO: 189), (X1)nHNAEGAP(X7)m (SEQ ID NO: 190), (X1)nKSAEGAP(X7)m (SEQ ID NO: 191), (X1)nKSDEGAP(X7)m (SEQ ID NO: 192), (X1)nKSEDGAP(X7)m (SEQ ID NO: 193), (X1)nRQDEGAP(X7)m (SEQ ID NO: 194), (X1)nRQEDGAP(X7)m (SEQ ID NO: 195), (X1)nHSAEGAP(X7)m (SEQ ID NO: 196), (X1)nRSAEGAP(X7)m (SEQ ID NO: 197), (X1)nRSDEGAP(X7)m (SEQ ID NO: 198), (X1)nRSEDGAP(X7)m (SEQ ID NO: 199), (X1)nHSDEGAP(X7)m (SEQ ID NO: 200), (X1)nHSEDGAP(X7)m (SEQ ID NO: 201) and (X1)nRQDDGAP(X7)m (SEQ ID NO: 202), wherein X1 and X7 is cysteine and n and m, independently, are 0 or 1.
According to a preferred embodiment of the present invention the peptide is coupled to a pharmaceutically acceptable carrier, preferably KLH (Keyhole Limpet Hemocyanin).
The pharmaceutical formulation according to the present invention, which can be formulated as a vaccine for, e.g., subcutaneous, intradermal and/or intramuscular administration, may be used in the treatment of any kind of synucleinopathy.
The present invention is further illustrated in the following figures and examples, however, without being restricted thereto.
FIG. 1 shows the sequence of full length alpha-synuclein (SEQ ID NO: 1) (140aa; swiss prot entry: P37840) and the sequence used to create a monoclonal antibody for detection of full length alpha-synuclein as well as C-terminally truncated and modified versions thereof. The peptide at position 100-109 used for generation of the monoclonal antibody is underlined. The peptide (p4453) was coupled to a C at C-terminal position.
FIG. 2 shows detection of alpha-synuclein by ELISA using the generated monoclonal antibody specific for human alpha-synuclein at position 100-109. The monoclonal antibody 12-9-9 was generated and tested for its specificity to synucleins in ELISA. Alpha-synuclein (p4446) and p4453 the human epitope are detected. The negative control protein p4447 (beta-synuclein) is not detected.
FIG. 3 shows the definition of the minimal epitope of the monoclonal antibody 12-9-9 by ELISA.
The peptides p4446 (alpha-synuclein), p4453 (human epitope used to create the antibody tested) are detected by the anti-body. The original epitope p4453 was N- or C- terminally truncated and used for ELISA to define the minimal epitope required for specific binding. The peptides p5399 and p5403 lost binding to the monoclonal antibody 12-9-9. Thus the minimal sequence needed for binding of 12-9-9 is predicted as KNEEGAP (SEQ ID NO: 139) located at position 102-108 of alpha-synuclein, while truncation of one of the flanking amino acids abolished the binding. Data are presented in a linear scale.
FIG. 4 shows detection of epitopes and mimotopes by ELISA using a monoclonal specific for human alpha-synuclein at position 100-109. Alpha-Synuclein as well as the peptides p5436 (human minimal epitope) and the mimotopes p5439 are detected similarly by the monoclonal antibody 12-9-9. The mimotope p5440 is not detected, while mimotope p5444 is detected much weaker than the human epitope by the monoclonal antibody 12-9-9.
FIG. 5 shows the induction of immune response against alpha-synuclein after peptide immunization. Sera of immunized mice (p5436 to p5590) show titers against alpha-synuclein after 3 vaccinations. Sera of immunized mice (p5463 to p5466) do not detect alpha synuclein (Titers measured in ELISA are around or below 1:50 half-max). Class of immunogenicity was defined as follows: Class 2: peptides inducing an immune response with OD halfmax higher than 1:1000. Class 1: peptides inducing an immune response with OD halfmax between 1000 and 51. Class 0: peptides inducing no or an very low immune response with OD halfmax around 50 or lower.
EXAMPLES
To identify peptides and polypeptides which can be used to treat and/or prevent synucleopathies an antibody was used, which is able to detect the human alpha-synuclein-derived amino acid sequence LGKNEEGAPQ (SEQ ID NO: 203) (=original epitope, SEQ ID No. 3, p4453) and full length human alpha-synuclein (SEQ ID No. 1, p4446). It does not recognize human beta-synuclein (SEQ ID No. 2, p4447; accession number Q16143: mdvfmkglsm akegvvaaae ktkqgvteaa ektkegvlyv gsktregvvq gvasvaektk eqashlggav fsgagniaaa tglvkreefp tdlkpeevaq eaaeepliep lmepegesye dppqeeyqey epea). The antibody may be a monoclonal or polyclonal antibody preparation or any antibody part or derivative thereof and binds specifically to the LGKNEEGAPQ epitope (SEQ ID NO: 203) of human alpha-synuclein, i.e. it does bind to peptide and full length protein but does not bind to human beta-synuclein. The mimotopes are identified and further characterised with such monoclonal antibodies (detecting a sequence within amino acids 100-109 of the human alpha-synuclein protein) and peptide libraries.
Example 1
Generation of Monoclonal Antibodies to Specifically Detect Original Human Alpha-Synuclein Epitope LGKNEEGAPQC SEQ ID No. 3, p4453 and Human Alpha-Synuclein but not Human Beta-Synuclein
A monoclonal antibody derived from the fusion “AFFiRiS 6”: Balb/c mice (Charles River) were immunized with original alpha-synuclein epitope LGKNEEGAPQ-C (SEQ ID NO:3) coupled to BTG (bovine thyroglobuline) and CFA (complete Freund's adjuvant; first injection) as well as IFA (incomplete Freund's adjuvant; 3 booster injections) as adjuvant. LGKNEEGAPQ-peptide-specific (SEQ ID NO: 203) antibody-producing hybridomas are detected by ELISA (LGKNEEGAPQC-peptide-coated (SEQ ID NO: 3)ELISA plates). Human alpha-synuclein (recombinant protein, p4446) is used as positive control peptide: hybridomas recognizing the recombinant protein immobilized on ELISA plates are included because they are binding both peptide and full length alpha-synuclein specifically. Human beta-synuclein (recombinant protein, p4447) is used as negative control peptide: hybridomas recognizing both recombinant proteins immobilized on ELISA plates are excluded because they do not distinguish between the two different synuclein proteins. The Hybridoma clone (12-9-9; IgG1, kappa) was analysed for specific detection of the natural human alpha-synuclein epitope LGKNEEGAPQ. (SEQ ID NO: 203). 12-9-9 recognizes the injected epitope as well as full length alpha-synuclein protein (recombinant protein; obtained from rPeptide, Bogart, Ga., USA) in ELISA (see FIG. 2). It however does not detect beta-synuclein protein (recombinant protein, obtained from rPeptide, Bogart, Ga., USA) in ELISA (see FIG. 2). Subsequently, the minimal epitope required for binding of the antibody has been defined by ELISA using the peptides p4446, p4453, p5397, p5398, p5399, p5400, p5401, p5402, p5403, p5404, p5405, p5406 (see FIG. 3) and p5436 (see FIG. 4). p4446, p4453, p5397, p5398 and p5402 as well as p5436 retained full binding capacities whereas p5399, p5400, p5401, p5403, p5404, p5405 and p5406 lost binding to 12-9-9. Thus the minimal required epitope for binding has been defined as KNEEGAP (SEQ ID NO: 139).
Example 2
Phage Display, In Vitro Binding and Inhibition ELISA
Phage Display libraries used in this example were: Ph.D. 7: New England BioLabs E8102L (linear 7mer library), Ph.D. 12: New England BioLabs E8111L (linear 12mer library) and Ph.D. C7C: New England BioLabs E8120L (a disulfide-constrained heptapeptide library) Phage Display was done according to manufacturer's protocol. After 2 or 3 subsequent rounds of panning, single phage clones were picked and phage supernatants were subjected to ELISA on plates coated with the antibody that was used for the panning procedure. Phage clones that were positive in this ELISA (strong signal for the target, but no signal for unspecific control) were sequenced. From DNA sequences, peptide sequences were deduced. These peptides were synthesized and characterised in binding and inhibition ELISA. To some peptides additional AA were attached to the C-terminus. Additionally, some novel mimotopes were created by combining sequence information from mimotopes identified in the screen. Both groups containing newly designed mimotopes were used to support the identification of a consensus sequence for a mimotope vaccination.
1. In vitro binding assay (ELISA)
Peptides derived from Phage Display as well as N-terminally truncated variants thereof were coupled to BSA and bound to ELISA plates (1 μM) and subsequently incubated with the monoclonal antibody that was used for the screening procedure to analyse binding capacity of identified peptides (see FIG. 4).
2. In vitro inhibition assay (ELISA)
Different amounts of peptides (concentrations ranging from 400 μg/ml to 3 μg/ml (serial dilutions), derived from Phage Display were incubated with the monoclonal antibody that was used for the screening procedure. Peptides diminishing subsequent binding of the antibody to the original human alpha-synuclein epitope (p5436) and the human alpha-synuclein protein (p4446) coated on ELISA plates were considered as inhibiting in this assay.
Example 3
In Vivo Testing of Mimotopes: Analysis of Immunogenicity
1. In vivo testing of mimotopes
Inhibiting as well as non-inhibiting peptides were coupled to KLH and injected into mice (wildtype C57/B16 or BalbC mice; subcutaneous injection into the flank) together with an appropriate adjuvant (aluminium hydroxide). Animals were vaccinated 3 times in biweekly intervals and sera were taken biweekly as well. Titers to injected peptides as well as to an irrelevant peptide were determined with every serum. Titers against the recombinant human alpha-synuclein protein and recombinant human beta-synuclein were determined starting with Serum 2 respectively. In general sera were analysed by reaction against peptides coupled to Bovine Serum Albumin (BSA) and recombinant full length proteins which were immobilised on ELISA plates. Titers were determined using anti mouse IgG specific antibodies. For examples of immunogenicity against injected peptide and alpha-synuclein see Table 5 and Table 6.
2. Results
2.1. Identification of an alpha-synuclein specific mAB: FIG. 2 depicts the characterisation of the alpha-synuclein specific monoclonal antibody 12-9-9 (IgG1, kappa) derived from fusion Affiris 6.
2.2. Screening for alpha-synuclein specific Mimotopes:
2.2.1. Phage Display PhD 7, PhD12 and PhD C7C and mutational Screen
2.2.1.1. Screening with monoclonal antibody directed against LGKNEEGAPQ (SEQ ID NO: 203)
By screening of PhD 7, PhD12 and PhD C7C phage display libraries and selective displacement of single amino acids a total of 60 sequences was identified (see Table 1; ID18-77).
Table 1 shows examples all peptides used.












SEQ




ID
peptide No
sequence







 1
p4446
Alpha-synuclein (FIG. 1; P37840)


 


 2
p4447
Beta-Synuclein; Q16143


 


 3
p4453
LGKNEEGAPQC


 


 4
p4454
MGKGEEGYPQC


 


 5
p5397
GKNEEGAPQC


 


 6
p5398
KNEEGAPQC


 


 7
p5399
NEEGAPQC


 


 8
p5400
EEGAPQC


 


 9
p5401
EGAPQC


 


10
p5402
LGKNEEGAPC


 


11
p5403
LGKNEEGAC


 


12
p5404
LGKNEEGC


 


13
p5405
LGKNEEC


 


14
p5406
LGKNEC


 


15
p5435
CKNEEGAP


 


16
p5436
KNEEGAPC


 


17
p1253
DAEFRHDSGY-C


 


18
p5437
ANEEGAPC


 


19
p5438
KAEEGAPC


 


20
p5439
KNAEGAPC


 


21
p5440
KNEAGAPC


 


22
p5441
KNEEAAPC


 


23
p5442
KNEEGAAC


 


24
p5443
RNEEGAPC


 


25
p5444
HNEEGAPC


 


26
p5445
KNDEGAPC


 


27
p5446
KNEDGAPC


 


28
p5447
KQEEGAPC


 


29
p5448
KSEEGAPC


 


30
p5449
KNDDGAPC


 


31
p5461
KPSFKNEC


 


32
p5462
QPSFAMEC


 


33
p5463
SPSFKQEC


 


34
p5464
TPSWKGEC


 


35
p5465
DPSFALEC


 


36
p5466
LPSFRLEC


 


37
p5467
EPNSRMDC


 


38
p5468
QPSSKLDC


 


39
p5469
HIHQSKFFDAPPC


 


40
p5547
QASFAMEC


 


41
p5548
TASWKGEC


 


42
p5549
QASSKLDC


 


43
p5550
QPAFAMEC


 


44
p5551
TPAWKGEC


 


45
p5552
QPASKLDC


 


46
p5553
QPSFAMAC


 


47
p5554
TPSWKGAC


 


48
p5555
QPSSKLAC


 


49
p5556
APSWKGEC


 


50
p5557
TPSAKGEC


 


51
p5558
TPSWAGEC


 


52
p5559
TPSWKAEC


 


53
p5560
CTPSWKGE


 


54
p5587
RNDEGAPC


 


55
p5588
RNEDGAPC


 


56
p5589
RQEEGAPC


 


57
p5590
RSEEGAPC


 


58
p5591
ANDEGAPC


 


59
p5592
ANEDGAPC


 


60
p5593
HSEEGAPC


 


61
p5594
ASEEGAPC


 


62
p5595
HNEDGAPC


 


63
p5596
HNDEGAPC


 


64
p5597
RNAEGAPC


 


65
p5598
HNAEGAPC


 


66
p5599
KSAEGAPC


 


67
p5600
KSDEGAPC


 


68
p5601
KSEDGAPC


 


69
p5602
RQDEGAPC


 


70
p5603
RQEDGAPC


 


71
p5604
HSAEGAPC


 


72
p5605
RSAEGAPC


 


73
p5606
RSDEGAPC


 


74
p5607
RSEDGAPC


 


75
p5608
HSDEGAPC


 


76
p5609
HSEDGAPC


 


77
p5610
RQDDGAPC









Table 2 shows examples of peptides and their binding capicity as compared to the original eptide.







TABLE 2







Examples of alpha-synuclein epi- and mimotopes


binding to the monoclonal Antibody 12-9-9











internal

SEQ




number 
Sequence
 ID NO:
Binding
remark





p5435
CKNEEGAP
15
2
original


 


p5436
KNEEGAPC
16
2
original


 


p5437
ANEEGAPC
18
2
mimotope


 


p5438
KAEEGAPC
19
1
mimotope


 


p5439
KNAEGAPC
20
2
mimotope


 


p5443
RNEEGAPC
24
2
mimotope


 


p5444
HNEEGAPC
25
2
mimotope


 


p5445
KNDEGAPC
26
2
mimotope


 


p5446
KNEDGAPC
27
2
mimotope


 


p5447
KQEEGAPC
28
2
mimotope


 


p5448
KSEEGAPC
29
2
mimotope


 


p5449
KNDDGAPC
30
2
mimotope


 


p5398
KNEEGAPQC
 6
2
original


 


p5402
LGKNEEGAPC
10
2
original


 


p5397
GKNEEGAPQC
 5
2
original


 


p4454
MGKGEEGYPQC
 4
2
original 






mouse


 


p4453
LGKNEEGAPQC
 3
2
original


 


p5461
KPSFKNEC
31
2
mimotope


 


p5462
QPSFAMEC
32
2
mimotope


 


p5463
SPSFKQEC
33
2
mimotope


 


p5464
TPSWKGEC
34
2
mimotope


 


p5465
DPSFALEC
35
2
mimotope


 


p5466
LPSFRLEC
36
1
mimotope


 


p5468
QPSSKLDC
38
2
mimotope


 


p5547
QASFAMEC
40
1
mimotope


 


p5548
TASWKGEC
41
2
mimotope


 


p5549
QASSKLDC
42
1
mimotope


 


p5551
TPAWKGEC
44
1
mimotope


 


p5556
APSWKGEC
49
2
mimotope


 


p5558
TPSWAGEC
51
2
mimotope


 


p5560
CTPSWKGE
53
2
mimotope


 


p5587
--RNDEGAPC
54
2
mimotope


 


p5588
--RNEDGAPC
55
2
mimotope


 


p5589
--RQEEGAPC
56
2
mimotope


 


p5590
--RSEEGAPC
57
2
mimotope


 


p5591
--ANDEGAPC
58
2
mimotope


 


p5593
--HSEEGAPC
60
1
mimotope


 


p5594
--ASEEGAPC
61
1
mimotope


 


p5596
--HNDEGAPC
63
2
mimotope


 


p5597
--RNAEGAPC
64
2
mimotope


 


p5598
--HNAEGAPC
65
1
mimotope


 


p5599
--KSAEGAPC
66
2
mimotope


 


p5600
--KSDEGAPC
67
2
mimotope


 


p5601
--KSEDGAPC
68
2
mimotope


 


p5602
--RQDEGAPC
69
2
mimotope


 


p5603
--RQEDGAPC
70
2
mimotope


 


p5604
--HSAEGAPC
71
1
mimotope


 


p5605
--RSAEGAPC
72
2
mimotope


 


p5606
--RSDEGAPC
73
2
mimotope


 


p5607
--RSEDGAPC
74
2
mimotope


 


p5608
--HSDEGAPC
75
1
mimotope


 


p5610
--RQDDGAPC
77
2
mimotope


 


p4446


2
α-Syn





the binding capacity is coded by the following code:


0: no binding to 12-9-9 detectable in ELISA


1: weak binding: binding of mimotope weaker compared to the minimal original sequence p5436


2: strong binding: binding of mimotope similar to the minimal original sequence p5436






2.3. In vitro characterisation of mimotopes identified in a screen (Phage Display and peptide screening) with a monoclonal antibody directed against alpha-synuclein:
FIGS. 2 and 3 show representative examples for binding and inhibition assays used to characterise mimotopes in vitro. Data obtained are summarised in Tables 2 and 3 respectively.
Table : Inhibition assay







TABLE 3







Inhibition assay


Alpha-synuclein mimotopes identified in 


this invention giving positive results


in inhibition assays 











desig-

SEQ




nation
Sequence
 ID NO:
Competition
remark





p5435
CKNEEGAP
15
1
original


 


p5436
KNEEGAPC
16
2
original


 


p5439
KNAEGAPC
20
1
mimotope


 


p5443
RNEEGAPC
24
2
mimotope


 


p5445
KNDEGAPC
26
2
mimotope


 


p5446
KNEDGAPC
27
1
mimotope


 


p5448
KSEEGAPC
29
1
mimotope


 


p5449
KNDDGAPC
30
1
mimotope


 


p5398
KNEEGAPQC
 6
2
original


 


p5402
LGKNEEGAPC
10
2
original


 


p5397
GKNEEGAPQC
 5
2
original 






mouse


 


p5464
TPSWKGEC
34
1
mimotope


 


p5548
TASWKGEC
41
1
mimotope


 


p5556
APSWKGEC
49
1
mimotope


 


p5557
TPSAKGEC
50
1
mimotope


 


p5587
RNDEGAPC
54
2
mimotope


 


p5588
RNEDGAPC
55
1
mimotope


 


p5590
RSEEGAPC
57
1
mimotope


 


p5597
RNAEGAPC
64
1
mimotope


 


p5600
KSDEGAPC
67
1
mimotope


 


p5602
RQDEGAPC
69
1
mimotope


 


p5603
RQEDGAPC
70
1
mimotope





Legend to Table 3: the competition capacity is coded by the following code:


0: no competition of 12-9-9 detectable in ELISA


1: weak competition: competition of mimotope weaker compared to the minimal original sequence p5436


2: strong competition: competition of mimotope similar to the minimal original sequence p5436













TABLE 4







Non-Mimotope peptides and proteins:









SEQ ID No.
designation
sequence












1
p4446
Alpha-synuclein


 


2
p4447
Beta-Synuclein


 


3
p4453
LGKNEEGAPQC


 


4
p4454
MGKGEEGYPQC


 


5
p5397
GKNEEGAPQC


 


6
p5398
KNEEGAPQC


 


7
p5399
NEEGAPQC


 


8
p5400
EEGAPQC


 


9
p5401
EGAPQC


 


10
p5402
LGKNEEGAPC


 


11
p5403
LGKNEEGAC


 


12
p5404
LGKNEEGC


 


13
p5405
LGKNEEC


 


14
p5406
LGKNEC


 


15
p5435
CKNEEGAP


 


16
p5436
KNEEGAPC


 


17
p1253
DAEFRHDSGY-C









2.4. In vivo characterisation of mimotopes identified in screening Phage Display Libraries with a monoclonal antibody directed against alpha-synuclein:
Female C57/B16 mice or BalbC, 5-6 mice per group, were subcutaneously immunized with 30 μg peptide coupled to KLH. Control groups were injected with PBS or the original epitope. As adjuvant alum was used. The peptides administered were all able to bind to monoclonal antibodies specifically binding aa100-109 of human alpha-synuclein although some of the peptides did inhibit the binding of the original epitope to its parental antibody in vitro only weakly (in an in vitro inhibition assay). The in vitro ELISA assay to determine the antibody titer was performed with sera of single mice (see Tab. 5) after each vaccination in a two week interval. The wells of the ELISA plate were coated with mimotope-BSA conjugates. The positive control was performed by reaction of the parental antibody with the respective mimotope-BSA conjugate. The detection was performed with anti-mouse IgG. Additionally, recombinant proteins were immobilised on ELISA plates and sera reacted accordingly. For all mimotopes tested in C57/B16 mice or BalbC, antibodies reacting to the individual injected peptide could be detected after repeated vaccination. Although not all mice induced higher titer against alpha-synuclein (see tab. 5 for examples).







TABLE 5







Induction of immune response is indicated by the


titer against injected peptide (p4446). Titer


was measured by ELISA and indicated as OD halfmax.














Titer in animals OD



designation
Sequence
SEQ ID NO:
halfmax injected pep.
remark














p5436
KNEEGAPC
16
10000
original


 


p5439
KNAEGAPC
20
8000
mimotope


 


p5443
RNEEGAPC
24
9000
mimotope


 


p5445
KNDEGAPC
26
26000
mimotope


 


p5402
LGKNEEGAPC
10
20000
original


 


p4454
MGKGEEGYPQC
 4
17000
original mouse
















TABLE 6







class of immunogenicity of mimotopes against a Syn














class of



desig-

SEQ
immunogenicity:



nation
sequence
 ID NO:
 a Syn
remark





p5402
LGKNEEGAP-C
10
2
original


 


p5436
KNEEGAPC
16
2
original


 


p5445
KNDEGAPC
26
2
mimotope


 


p5443
RNEEGAPC
24
1
mimotope


 


p5587
RNDEGAPC
54
1
mimotope


 


p5439
KNAEGAPC
20
1
mimotope


 


p5600
KSDEGAPC
67
1
mimotope


 


p4454
MGKGEEGYPQC
 4
1
original






mouse


 


p5597
RNAEGAPC
64
1
mimotope


 


p5590
RSEEGAPC
57
1
mimotope


 


p5463
SPSFKQEC
33
0
mimotope


 


p5556
APSWKGEC
49
0
mimotope


 


p5462
QPSFAMEC
32
0
mimotope


 


p5464
TPSWKGEC
34
0
mimotope


 


p5468
QPSSKLDC
38
0
mimotope


 


p5461
KPSFKNEC
31
0
mimotope


 


p5465
DPSFALEC
35
0
mimotope


 


p5466
LPSFRLEC
36
0
mimotope





Class of immunogenicity:


peptides were ranked according to their capacity to induce an immune response


2: peptides inducing an immune response with OD halfmax higher than 1000.


1: peptides inducing an immune response with OD halfmax between 1000 and 51.


0: peptides inducing no immune response or a very low immune response with OD halfmax around 50 or lower.

## Claims

1. A composition comprising a peptide or polypeptide comprising the amino acid sequence


(X1)nX2X3X4X5GX6P(X7)m(SEQ ID NO: 78)  (Formula I),

wherein
X1 is any amino acid residue,
X2 is an amino acid residue selected from the group consisting of lysine (K), arginine (R), alanine (A) and histidine (H),
X3 is an amino acid residue selected from the group consisting of asparagine (N), glutamine (Q), serine (S), glycine (G) and alanine (A),
X4 is an amino acid residue selected from the group consisting of glutamic acid (E), aspartic acid (D) and alanine (A),
X5 is an amino acid residue selected from the group consisting of glutamic acid (E) and aspartic acid (D),
X6 is an amino acid residue selected from the group consisting of alanine (A) and tyrosine (Y),
X7 is any amino acid residue,
n and m, independently, are 0 or an integer of more than 0,
and wherein the amino acid sequence according to Formula I is not identical with, or does not comprise the 7-mer polypeptide fragment of alpha-synuclein having the amino acid sequence KNEEGAP (SEQ ID NO: 139) and wherein the peptide or polypeptide does not comprise the amino acid sequence of formula I wherein X2 is K, X3 is G, X4 is E, X5 is E, X6 is Y and X7 is P,
said at least one peptide or polypeptide having a binding capacity to an antibody which is specific for an epitope of alpha-synuclein comprising the amino acid sequence KNEEGAP (SEQ ID NO: 139),
suitable for use in preventing and/or treating synucleinopathies.

2. The composition according to claim 1, wherein X2 is an amino acid residue selected from the group consisting of lysine (K) and arginine (R) and/or X6 is alanine (A) (SEQ ID NO: 204).

3. The composition according to claim 1 wherein the peptide or polypeptide comprises an amino acid sequence selected from the group consisting of (X1)nKNDEGAP(X7)m(SEQ ID NO: 87), (X1)nANEEGAP(X7)m(SEQ ID NO: 79), (X1)nKAEEGAP(X7)m(SEQ ID NO: 80), (X1)nKNAEGAP(X7)m(SEQ ID NO: 81), (X1)nRNEEGAP(X7)m, (SEQ ID NO: 85), (X1)nHNEEGAP(X7)m(SEQ ID NO: 86), (X1)nKNEDGAP(X7)m, (SEQ ID NO: 88), (X1)nKQEEGAP(X7)m(SEQ ID NO: 89), (X1)nKSEEGAP(X7)m, (SEQ ID NO: 90), (X1)nKNDDGAP (X7)m, (SEQ ID NO: 91), (X1)nRNDEGAP(X7)m, (SEQ ID NO: 115), (X1)nRNEDGAP(X7)m(SEQ ID NO: 116), (X1)nRQEEGAP(X7)m, (SEQ ID NO: 117),(X1)nRSEEGAP(X7)m(SEQ ID NO: 118), (X1)nANDEGAP(X7)m(SEQ ID NO: 119), (X1)nANEDGAP(X7)m(SEQ ID NO: 120), (X1)nHSEEGAP(X7)m, (SEQ ID NO: 121), (X1)nASEEGAP(X7)m(SEQ ID NO: 122), (X1)nHNEDGAP(X7)m, (SEQ ID NO: 123), (X1)nHNDEGAP(X7)m(SEQ ID NO: 124), (X1)nRNAEGAP(X7)m, (SEQ ID NO: 125), (X1)nHNAEGAP(X7)m(SEQ ID NO: 126), (X1)nKSAEGAP(X7)m(SEQ ID NO: 127), (X1)nKSDEGAP(X7)m(SEQ ID NO: 128), (X1)nKSEDGAP(X7)m(SEQ ID NO: 129), (X1)nRQDEGAP(X7)m(SEQ ID NO: 141), (X1)nRQEDGAP(X7)m(SEQ ID NO: 131), (X1)nHSAEGAP(X7)m, (SEQ ID NO: 132), (X1)nRSAEGAP(X7)m(SEQ ID NO: 133), (X1)nRSDEGAP(X7)m(SEQ ID NO: 134), (X1)nRSEDGAP(X7)m(SEQ ID NO: 135),(X1)nHSDEGAP(X7)m(SEQ ID NO: 136), (X1)nHSEDGAP(X7)m, (SEQ ID NO: 137), and (X1)nRQDDGAP(X7)m, (SEQ ID NO: 138).

4. The composition according to claim 1, wherein
n and/or m are 1, and
X1 and/or X7 are cysteine (C).

5. The composition according to claim 1, wherein the peptide or polypeptide consists of 7 to 30 amino acid residues.

6. The composition according to claim 1, wherein the synucleinopathy is selected from the group consisting of Lewy Body Disorders (LBDs), Multiple System Atrophy (MSA) and Neurodegeneration with Brain Iron Accumulation type I (NBIA Type I).

7. The composition according to claim 1, wherein the at least one peptide or polypeptide is coupled to a pharmaceutically acceptable carrier.

8. The composition according to claim 1, wherein the at least one peptide or polypeptide is formulated for intravenous, subcutaneous, intradermal or intramuscular administration.

9. The composition according to claim 1, wherein the at least one peptide or polypeptide is formulated with an adjuvant.

10. The composition according to claim 1, comprising 0.1 ng to 10 mg of the at least one peptide or polypeptide.

11. A peptide having an amino acid sequence selected from the group consisting of (X1)nKNDEGAP(X7)m(SEQ ID NO: 143), (X1)nANEEGAP(X7)m(SEQ ID NO: 144), (X1)nKAEEGAP(X7)m(SEQ ID NO: 145), (X1)nKNAEGAP(X7)m (SEQ ID NO: 146), (X1)nRNEEGAP(X7)m (SEQ ID NO: 150), (X1)nHNEEGAP(X7)m (SEQ ID NO: 151), (X1)nKNEDGAP(X7)m (SEQ ID NO: 152), (X1)nKQEEGAP(X7)m (SEQ ID NO: 153), (X1)nKSEEGAP(X7)m(SEQ ID NO: 154), (X1)nKNDDGAP(X7)m(SEQ ID NO: 155), (X1)nRNDEGAP(X7)m (SEQ ID NO: 179), (X1)nRNEDGAP(X7)m (SEQ ID NO: 180), (X1)nRQEEGAP(X7)m (SEQ ID NO: 181), (X1)nRSEEGAP(X7)m (SEQ ID NO: 182),(X1)nANDEGAP(X7)m (SEQ ID NO: 183), (X1)nANEDGAP(X7)m(SEQ ID NO: 184), (X1)nHSEEGAP(X7)m (SEQ ID NO: 185), (X1)nASEEGAP(X7)m(SEQ ID NO: 186), (X1)nHNEDGAP(X7)m(SEQ ID NO: 187), (X1)nHNDEGAP(X7)m(SEQ ID NO: 188), (X1)nRNAEGAP(X7)m (SEQ ID NO: 189), (X1)nHNAEGAP(X7)m(SEQ ID NO: 190), (X1)nKSAEGAP(X7)m (SEQ ID NO: 191), (X1)nKSDEGAP(X7)m(SEQ ID NO: 192), (X1)nKSEDGAP(X7)m (SEQ ID NO: 193), (X1)nRQDEGAP(X7)m (SEQ ID NO: 194), (X1)nRQEDGAP(X7)m (SEQ ID NO: 195), (X1)nHSAEGAP(X7)m (SEQ ID NO: 196), (X1)nRSAEGAP(X7)m (SEQ ID NO: 197), (X1)nRSDEGAP(X7), (SEQ ID NO: 198), (X1)nRSEDGAP(X7)m (SEQ ID NO: 199), (X1)nHSDEGAP(X7)m (SEQ ID NO: 200), (X1)nHSEDGAP(X7), (SEQ ID NO: 201) and (X1)nRQDDGAP(X7)m (SEQ ID NO: 202), wherein X1 and X7 are each cysteine and n and m, independently, are 0 or 1.