13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J12030116

Lab Contact: Jason C Perkins

Date: 3/23/2012

Customer Address: 253 Plant Allen Road

Customer Name(s): BILL KENNEDY, RON LAWS, ALLEN STOWE, ROBBIN JOLLY, DON SCRUG

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis,

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Analytical Lab 

Page 1 of 47
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2012005462 ALLEN 04-Mar-12 9:24 AM Jeremy D. FGD Purge Eff 

2012005463 ALLEN 04-Mar-12 8:13 AM Jeremy D. EQ Tank Eff 

2012005464 ALLEN 04-Mar-12 8:20 AM JLD/JBW BioReactor 1 Inf 

2012005465 ALLEN 04-Mar-12 8:20 AM JLD/JBW BioReactor 1 Inf BLANK 

2012005466 ALLEN 04-Mar-12 9:40 AM JLD/JBW BioReactor 2 Inf 

2012005467 ALLEN 04-Mar-12 9:40 AM JLD/JBW BioReactor 2 Inf BLANK 

2012005468 ALLEN 04-Mar-12 8:30 AM JLD/JBW BioReactor 2 Eff 

2012005469 ALLEN 04-Mar-12 8:30 AM JLD/JBW BioReactor 2 Eff BLANK 

2012005470 ALLEN 04-Mar-12 10:15 AM Jeremy D. Filter Blk 

9 Total Samples

Analytical Lab 

Page 2 of 47
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

The Vendor Laboratories have been qualified by the 

Analytical Laboratory

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: Mary Ann Ogle Date: 3/23/2012

Yes

Electronic Data Deliverable (EDD) Sent Separately

Analytical Lab 

Page 3 of 47
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12030116

2012005462

Collection Date: 04-Mar-12 9:24 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter COMPLETE 1 V_PRISM

INORGANIC IONS BY IC

Bromide 830 mg/L EPA 300.0 50 500 14-Mar-12 09:19 JAHERMA

Chloride 3000 mg/L EPA 300.0 50 500 14-Mar-12 09:19 JAHERMA

Sulfate 1200 mg/L EPA 300.0 50 500 14-Mar-12 09:19 JAHERMA

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 51.7 ug/L EPA 245.1 2.5 50 13-Mar-12 09:42 AGIBBS

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 2.50 ug/L EPA 245.1 2.5 50 13-Mar-12 11:07 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 38.6 mg/L EPA 200.7 0.5 10 13-Mar-12 14:22 MHH7131

Calcium (Ca) 2490 mg/L EPA 200.7 0.1 10 13-Mar-12 14:22 MHH7131

Iron (Fe) 91.7 mg/L EPA 200.7 0.1 10 13-Mar-12 14:22 MHH7131

Lithium (Li) 0.182 mg/L EPA 200.7 0.05 10 13-Mar-12 14:22 MHH7131

Magnesium (Mg) 393 mg/L EPA 200.7 0.05 10 13-Mar-12 14:22 MHH7131

Manganese (Mn) 5.88 mg/L EPA 200.7 0.05 10 13-Mar-12 14:22 MHH7131

Potassium (K) 36.9 mg/L EPA 200.7 1 10 13-Mar-12 14:22 MHH7131

Sodium (Na) 25.8 mg/L EPA 200.7 0.5 10 13-Mar-12 14:22 MHH7131

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 5440 ug/L EPA 200.8 10 10 16-Mar-12 13:49 KRICHAR

Selenium (Se) 1090 ug/L EPA 200.8 10 10 16-Mar-12 13:49 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 209 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Cadmium (Cd) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Chromium (Cr) 172 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Copper (Cu) 177 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Nickel (Ni) 181 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Selenium (Se) 6790 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Silver (Ag) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

Zinc (Zn) 214 ug/L EPA 200.8 10 10 14-Mar-12 11:27 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

Analytical Lab 

Page 4 of 47
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12030116

2012005462

Collection Date: 04-Mar-12 9:24 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL DISSOLVED SOLIDS

Vendor Parameter Complete 1 V_PACE

TOTAL SUSPENDED SOLIDS

Vendor Parameter Complete 1 V_PACE

2012005463

Collection Date: 04-Mar-12 8:13 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 31.5 ug/L EPA 245.1 2.5 50 13-Mar-12 09:49 AGIBBS

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 2.50 ug/L EPA 245.1 2.5 50 13-Mar-12 11:09 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 31.6 mg/L EPA 200.7 0.5 10 13-Mar-12 14:26 MHH7131

Calcium (Ca) 1610 mg/L EPA 200.7 0.1 10 13-Mar-12 14:26 MHH7131

Iron (Fe) 62.1 mg/L EPA 200.7 0.1 10 13-Mar-12 14:26 MHH7131

Lithium (Li) 0.129 mg/L EPA 200.7 0.05 10 13-Mar-12 14:26 MHH7131

Magnesium (Mg) 291 mg/L EPA 200.7 0.05 10 13-Mar-12 14:26 MHH7131

Manganese (Mn) 4.07 mg/L EPA 200.7 0.05 10 13-Mar-12 14:26 MHH7131

Potassium (K) 25.0 mg/L EPA 200.7 1 10 13-Mar-12 14:26 MHH7131

Sodium (Na) 20.3 mg/L EPA 200.7 0.5 10 13-Mar-12 14:26 MHH7131

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 3740 ug/L EPA 200.8 10 10 16-Mar-12 13:53 KRICHAR

Selenium (Se) 215 ug/L EPA 200.8 10 10 16-Mar-12 13:53 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 141 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Cadmium (Cd) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Chromium (Cr) 115 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Copper (Cu) 128 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Nickel (Ni) 134 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Selenium (Se) 3560 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Silver (Ag) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Zinc (Zn) 155 ug/L EPA 200.8 10 10 14-Mar-12 11:30 KRICHAR

Analytical Lab 

Page 5 of 47
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12030116

2012005464

Collection Date: 04-Mar-12 8:20 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter COMPLETE 1 V_PRISM

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) < 1.00 ug/L EPA 245.1 1 20 13-Mar-12 09:51 AGIBBS

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 2.50 ug/L EPA 245.1 2.5 50 13-Mar-12 11:11 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 32.3 mg/L EPA 200.7 0.5 10 13-Mar-12 14:30 MHH7131

Calcium (Ca) 1340 mg/L EPA 200.7 0.1 10 13-Mar-12 14:30 MHH7131

Iron (Fe) < 0.100 mg/L EPA 200.7 0.1 10 13-Mar-12 14:30 MHH7131

Lithium (Li) 0.056 mg/L EPA 200.7 0.05 10 13-Mar-12 14:30 MHH7131

Magnesium (Mg) 239 mg/L EPA 200.7 0.05 10 13-Mar-12 14:30 MHH7131

Manganese (Mn) 0.383 mg/L EPA 200.7 0.05 10 13-Mar-12 14:30 MHH7131

Potassium (K) 9.99 mg/L EPA 200.7 1 10 13-Mar-12 14:30 MHH7131

Sodium (Na) 18.6 mg/L EPA 200.7 0.5 10 13-Mar-12 14:30 MHH7131

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 376 ug/L EPA 200.8 10 10 16-Mar-12 13:57 KRICHAR

Selenium (Se) 68.3 ug/L EPA 200.8 10 10 16-Mar-12 13:57 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Cadmium (Cd) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Chromium (Cr) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Copper (Cu) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Nickel (Ni) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Selenium (Se) 66.9 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Silver (Ag) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

Zinc (Zn) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:33 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

Analytical Lab 

Page 6 of 47
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12030116

2012005465

Collection Date: 04-Mar-12 8:20 AM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

2012005466

Collection Date: 04-Mar-12 9:40 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter COMPLETE 1 V_PRISM

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) < 1.00 ug/L EPA 245.1 1 20 13-Mar-12 09:54 AGIBBS

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 2.50 ug/L EPA 245.1 2.5 50 13-Mar-12 11:14 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 31.1 mg/L EPA 200.7 0.5 10 13-Mar-12 14:34 MHH7131

Calcium (Ca) 1280 mg/L EPA 200.7 0.1 10 13-Mar-12 14:34 MHH7131

Iron (Fe) 7.95 mg/L EPA 200.7 0.1 10 13-Mar-12 14:34 MHH7131

Lithium (Li) 0.055 mg/L EPA 200.7 0.05 10 13-Mar-12 14:34 MHH7131

Magnesium (Mg) 215 mg/L EPA 200.7 0.05 10 13-Mar-12 14:34 MHH7131

Manganese (Mn) 0.625 mg/L EPA 200.7 0.05 10 13-Mar-12 14:34 MHH7131

Potassium (K) 10.7 mg/L EPA 200.7 1 10 13-Mar-12 14:34 MHH7131

Sodium (Na) 17.5 mg/L EPA 200.7 0.5 10 13-Mar-12 14:34 MHH7131

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 599 ug/L EPA 200.8 10 10 16-Mar-12 14:01 KRICHAR

Selenium (Se) 14.6 ug/L EPA 200.8 10 10 16-Mar-12 14:01 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Cadmium (Cd) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Chromium (Cr) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Copper (Cu) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Nickel (Ni) 17.3 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Selenium (Se) 16.7 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Silver (Ag) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Zinc (Zn) < 10.0 ug/L EPA 200.8 10 10 14-Mar-12 11:36 KRICHAR

Analytical Lab 

Page 7 of 47
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12030116

2012005466

Collection Date: 04-Mar-12 9:40 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

2012005467

Collection Date: 04-Mar-12 9:40 AM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

2012005468

Collection Date: 04-Mar-12 8:30 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter COMPLETE 1 V_PRISM

INORGANIC IONS BY IC

Bromide 18 mg/L EPA 300.0 5 50 12-Mar-12 23:59 JAHERMA

Chloride 1600 mg/L EPA 300.0 50 500 12-Mar-12 23:59 JAHERMA

Sulfate 1300 mg/L EPA 300.0 50 500 12-Mar-12 23:59 JAHERMA

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) < 1.00 ug/L EPA 245.1 1 20 13-Mar-12 09:56 AGIBBS

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 2.50 ug/L EPA 245.1 2.5 50 13-Mar-12 11:16 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 29.3 mg/L EPA 200.7 0.5 10 13-Mar-12 14:38 MHH7131

Calcium (Ca) 1120 mg/L EPA 200.7 0.1 10 13-Mar-12 14:38 MHH7131

Iron (Fe) 0.743 mg/L EPA 200.7 0.1 10 13-Mar-12 14:38 MHH7131

Lithium (Li) < 0.050 mg/L EPA 200.7 0.05 10 13-Mar-12 14:38 MHH7131

Magnesium (Mg) 164 mg/L EPA 200.7 0.05 10 13-Mar-12 14:38 MHH7131

Manganese (Mn) 0.816 mg/L EPA 200.7 0.05 10 13-Mar-12 14:38 MHH7131

Potassium (K) 16.4 mg/L EPA 200.7 1 10 13-Mar-12 14:38 MHH7131

Sodium (Na) 14.8 mg/L EPA 200.7 0.5 10 13-Mar-12 14:38 MHH7131

Analytical Lab 

Page 8 of 47
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12030116

2012005468

Collection Date: 04-Mar-12 8:30 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 776 ug/L EPA 200.8 5 5 16-Mar-12 14:05 KRICHAR

Selenium (Se) < 5.00 ug/L EPA 200.8 5 5 16-Mar-12 14:05 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5.00 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Cadmium (Cd) < 5.00 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Chromium (Cr) < 5.00 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Copper (Cu) < 5.00 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Nickel (Ni) 68.6 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Selenium (Se) < 5.00 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Silver (Ag) < 5.00 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

Zinc (Zn) 6.41 ug/L EPA 200.8 5 5 14-Mar-12 11:39 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

2012005469

Collection Date: 04-Mar-12 8:30 AM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

2012005470

Collection Date: 04-Mar-12 10:15 AM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) < 0.05 ug/L EPA 245.1 0.05 1 13-Mar-12 11:40 AGIBBS

DISSOLVED METALS BY ICP-MS

Manganese (Mn) < 1.00 ug/L EPA 200.8 1 1 16-Mar-12 12:58 KRICHAR

Selenium (Se) < 1.00 ug/L EPA 200.8 1 1 16-Mar-12 12:58 KRICHAR

Analytical Lab 

Page 9 of 47
 

March 14, 2012 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen HAPS/MACT (Wastewater) (LIMS # J12030116) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation analysis on March 6, 2012. The samples were received in a sealed cooler at -0.5°C on 

March 7, 2012. Selenium speciation analysis was performed via ion chromatography inductively 

coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). Any issues 

associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

 

Analytical Lab 

Page 10 of 47
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen HAPS/MACT (Wastewater) (LIMS # J12030116) 

March 14, 2012 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on March 6, 2012. The samples 

were received on March 7, 2012 in a sealed container at -0.5°C. 

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and was designated a discrete sample identifier. An aliquot of each 

sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored cryofreezer 

(maintained at a temperature of -80oC) until selenium speciation analysis could be performed 

via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry 

(IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed 

autosampler vial. No further sample preparation was performed as any chemical alteration of 

a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

each analytical day. All calibration curves, associated with each species of interest, are 

Analytical Lab 

Page 11 of 47
standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on March 12, 2012. An aliquot of each sample is

injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The 

eluting selenium species are then introduced into a radio frequency (RF) plasma where 

energy-transfer processes cause desolvation, atomization, and ionization. The ions are 

extracted from the plasma through a differentially-pumped vacuum interface and travel 

through a pressurized chamber (CRC) containing hydrogen gas which preferentially reacts 

with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector 

detects ions transmitted through the mass analyzer and the resulting current is processed by a 

data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with this sample were within acceptance limits.

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Analytical Lab 

Page 12 of 47
If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Analytical Lab 

Page 13 of 47
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff 1310 52.1 17.9 29.5 ND (<2.3) 107 (2)

BioReactor 1 Inf 9.02 35.2 ND (<0.39) 6.93 ND (<0.57) 8.06 (1)

BioReactor 2 Inf 6.82 ND (<0.54) ND (<0.39) 0.79 ND (<0.57) 0.98 (1)

BioReactor 2 Eff ND (<0.77) ND (<0.54) ND (<0.39) ND (<0.57) ND (<0.57) 0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

n = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen HAPS/MACT (Wastewater)

Contact: Jay Perkins

Date: March 14, 2012

LIMS #J12030116

Analytical Lab 

Page 14 of 47
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.77 3.1

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.54 2.2

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.39 1.5

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.57 2.3

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.57 2.3

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 9.81 102.5

Se(VI) LCS 9.48 9.10 96.0

SeCN LCS 8.92 8.69 97.5

MeSe(IV) LCS 6.47 5.78 89.3

SeMe LCS 9.32 8.62 92.5

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen HAPS/MACT (Wastewater)

Contact: Jay Perkins

LIMS #J12030116

Date: March 14, 2012

Report Generated by: Russell Gerads

Analytical Lab 

Page 15 of 47
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) Batch QC 13.45 13.03 13.24 3.2

Se(VI) Batch QC 35.78 35.21 35.50 1.6

SeCN Batch QC ND (<0.39) ND (<0.39) NC NC

MeSe(IV) Batch QC 14.55 14.92 14.74 2.5

SeMe Batch QC ND (<0.57) ND (<0.57) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

*Sample concentrations are within 10x the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) Batch QC 1390 2168 155.0 1390 2101 150.2 3.2

Se(VI) Batch QC 1261 1313 101.3 1261 1307 100.8 0.5

SeCN Batch QC 1144 483.3 42.3* 1144 532.3 46.5* 9.6

*Low recovery is attributed to matrix induced species conversion

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen HAPS/MACT (Wastewater)

Contact: Jay Perkins

LIMS #J12030116

Date: March 14, 2012

Report Generated by: Russell Gerads

Analytical Lab 

Page 16 of 47
Analytical Lab 

Page 17 of 47
 

3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

March 14, 2012 

Duke Energy 

ATTN: Jay Perkins 

Scientific Support-Laboratory

13339 Hagers Ferry Road 

Huntersville NC 28078 

jcperkins@duke-energy.com 

labcustomer@duke-energy.com 

RE: Project DUK-HV1201 Client Project: J12030116 

Dear Mr. Perkins, 

On March 7, 2012, Brooks Rand Labs (BRL) received three (3) wastewater samples and three 

(3) corresponding field blanks. Samples were logged-in for total mercury (Hg) analysis. All 

samples were received, prepared, analyzed, and stored according to BRL SOPs and EPA 

methodology. 

The results were blank-corrected as described in the calculations section of the applicable 

SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. 

Please refer to the Sample Results page for sample-specific detection limits and other details. 

Aside from concentration qualifiers, all data was reported without qualification and all associated 

quality control sample results met the acceptance criteria. 

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. Please feel free to contact me if you have any questions regarding this 

report. 

Sincerely, 

 

Tiffany Stilwater 

Project Manager 

tiffany@brooksrand.com 

Analytical Lab 

Page 18 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic 

Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 19 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1210024-01 Influent Sample 03/04/2012 03/07/2012

BioReactor 1 Inf Hg Blk 1210024-02 DIW Field Blank 03/04/2012 03/07/2012

BioReactor 2 Inf 1210024-03 Influent Sample 03/04/2012 03/07/2012

BioReactor 2 Inf Hg Blk 1210024-04 DIW Field Blank 03/04/2012 03/07/2012

BioReactor 2 Eff 1210024-05 Effluent Sample 03/04/2012 03/07/2012

BioReactor 2 Eff Hg Blk 1210024-06 DIW Field Blank 03/04/2012 03/07/2012

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 03/09/2012 03/12/2012 B120366 1200164

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 20 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Fraction Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1210024-01 Hg Influent T 365 3.03 8.08 ng/L B120366 1200164

BioReactor 1 Inf Hg Blk

1210024-02 Hg DIW T 0.15 U 0.15 0.40 ng/L B120366 1200164

BioReactor 2 Eff

1210024-05 Hg Effluent T 127 0.58 1.54 ng/L B120366 1200164

BioReactor 2 Eff Hg Blk

1210024-06 Hg DIW T 0.15 U 0.15 0.40 ng/L B120366 1200164

BioReactor 2 Inf

1210024-03 Hg Influent T 260 3.06 8.16 ng/L B120366 1200164

BioReactor 2 Inf Hg Blk

1210024-04 Hg DIW T 0.15 U 0.15 0.40 ng/L B120366 1200164

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 21 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B120366

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B120366-SRM1 Certified Reference Material (1209009, NIST 1641d 1000x dilution)

Hg 15.68 14.73 ng/L 94% 85-115

B120366-MS3 Matrix Spike (1210024-01)

Hg 365.2 1212 1773 ng/L 116% 71-125

B120366-MSD3 Matrix Spike Duplicate (1210024-01)

Hg 365.2 1212 1658 ng/L 107% 71-125 247%

Method Blanks & Reporting Limits

Batch: B120366

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B120366-BLK1 0.07 ng/L

B120366-BLK2 0.07 ng/L

B120366-BLK3 0.05 ng/L

B120366-BLK4 0.04 ng/L

Average: 0.06 Standard Deviation: 0.02 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 22 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200164

Instrument: THG-10 Method: EPA 1631

Date: 03/12/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200164-IBL1 5.96 pg of Hg

1200164-IBL2 5.89 pg of Hg

1200164-IBL3 5.92 pg of Hg

1200164-IBL4 5.80 pg of Hg

1200164-CAL1 25.00 25.66 pg of Hg 103%

1200164-CAL2 100.0 96.61 pg of Hg 97%

1200164-CAL3 500.0 485.2 pg of Hg 97%

1200164-CAL4 2500 2636 pg of Hg 105%

1200164-CAL5 10000 9885 pg of Hg 99%

1200164-ICV1 1568 1473 pg of Hg 94% 85-115

1200164-CCV1 500.0 484.5 pg of Hg 97% 77-123

1200164-CCB1 12.9 pg of Hg

1200164-CCV2 500.0 475.1 pg of Hg 95% 77-123

1200164-CCV3 500.0 483.3 pg of Hg 97% 77-123

1200164-CCV4 500.0 491.9 pg of Hg 98% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 23 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1210024-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 03/07/2012

Collected: 03/04/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250mL none n/a Cooler

Lab ID: 1210024-02 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 03/07/2012

Collected: 03/04/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250mL none n/a Cooler

Lab ID: 1210024-03 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 03/07/2012

Collected: 03/04/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250mL none n/a Cooler

Lab ID: 1210024-04 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 03/07/2012

Collected: 03/04/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250mL none n/a Cooler

Lab ID: 1210024-05 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 03/07/2012

Collected: 03/04/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250mL none n/a Cooler

Lab ID: 1210024-06 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 03/07/2012

Collected: 03/04/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250mL none n/a Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 24 of 47
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Shipping Containers

Cooler

Tracking No: 4726 7966 9021 via FedEx

Temperature: ambient

Coolant Type: None

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: March 7, 2012 8:45

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 25 of 47
Analytical Lab 

Page 26 of 47
March 12, 2012

LIMS USE: FR - TERRY WHITNER

LIMS OBJECT ID: 92113411

92113411

Project:

Pace Project No.:

RE:

Terry Whitner

Duke Energy Carolinas, LLC

PO Box 37929

DPEHS

Charlotte, NC 28237

J12030116

Dear Terry Whitner:

Enclosed are the analytical results for sample(s) received by the laboratory on March 06, 2012. The

results relate only to the samples included in this report. Results reported herein conform to the

most current TNI standards and the laboratory's Quality Assurance Manual, where applicable, unless

otherwise noted in the body of the report.

Analyses were performed at the Pace Analytical Services location indicated on the sample analyte

page for analysis unless otherwise footnoted.

If you have any questions concerning this report, please feel free to contact me.

Sincerely,

Kevin Herring

kevin.herring@pacelabs.com

Project Manager

Enclosures

cc: Mr. Jay Perkins, Duke Energy

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 1 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 27 of 47
CERTIFICATIONS

Pace Project No.:

Project:

92113411

J12030116

Asheville Certification IDs

2225 Riverside Dr., Asheville, NC 28804

Florida/NELAP Certification #: E87648

Massachusetts Certification #: M-NC030

North Carolina Drinking Water Certification #: 37712

North Carolina Wastewater Certification #: 40

South Carolina Certification #: 99030001

Virginia Certification #: 00072

West Virginia Certification #: 356

Virgina/VELAP Certification #: 460147

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 2 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 28 of 47
SAMPLE ANALYTE COUNT

Pace Project No.:

Project:

92113411

J12030116

Lab ID Sample ID Method

Analytes

Analysts Reported Laboratory

92113411001 FGD PURGE EFF SM 2540C SMW 1 PASI-A

SM 2540D SMW 1 PASI-A

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 3 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 29 of 47
HITS ONLY

Pace Project No.:

Project:

92113411

J12030116

Parameters Result Analyzed

Lab Sample ID 

Method Units Report Limit Qualifiers

Client Sample ID

92113411001 FGD PURGE EFF

SM 2540C Total Dissolved Solids 39700 mg/L 500 03/08/12 13:13

SM 2540D Total Suspended Solids 7100 mg/L 250 03/09/12 12:38

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 4 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 30 of 47
PROJECT NARRATIVE

Pace Project No.:

Project:

92113411

J12030116

Method:

Client: Duke Energy

SM 2540C

Date: March 12, 2012

Description: 2540C Total Dissolved Solids

General Information:

1 sample was analyzed for SM 2540C. All samples were received in acceptable condition with any exceptions noted below.

Hold Time:

The samples were analyzed within the method required hold times with any exceptions noted below.

Method Blank:

All analytes were below the report limit in the method blank with any exceptions noted below.

Laboratory Control Spike:

All laboratory control spike compounds were within QC limits with any exceptions noted below.

Matrix Spikes:

All percent recoveries and relative percent differences (RPDs) were within acceptance criteria with any exceptions noted below.

Duplicate Sample:

All duplicate sample results were within method acceptance criteria with any exceptions noted below.

QC Batch: WET/19999

D6: The relative percent difference (RPD) between the sample and sample duplicate exceeded laboratory control limits.

• DUP (Lab ID: 731931)

• Total Dissolved Solids

Additional Comments:

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 5 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 31 of 47
PROJECT NARRATIVE

Pace Project No.:

Project:

92113411

J12030116

Method:

Client: Duke Energy

SM 2540D

Date: March 12, 2012

Description: 2540D Total Suspended Solids

General Information:

1 sample was analyzed for SM 2540D. All samples were received in acceptable condition with any exceptions noted below.

Hold Time:

The samples were analyzed within the method required hold times with any exceptions noted below.

Method Blank:

All analytes were below the report limit in the method blank with any exceptions noted below.

Laboratory Control Spike:

All laboratory control spike compounds were within QC limits with any exceptions noted below.

Matrix Spikes:

All percent recoveries and relative percent differences (RPDs) were within acceptance criteria with any exceptions noted below.

Duplicate Sample:

All duplicate sample results were within method acceptance criteria with any exceptions noted below.

QC Batch: WET/20019

D6: The relative percent difference (RPD) between the sample and sample duplicate exceeded laboratory control limits.

• DUP (Lab ID: 732655)

• Total Suspended Solids

Additional Comments:

This data package has been reviewed for quality and completeness and is approved for release.

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 6 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 32 of 47
ANALYTICAL RESULTS

Pace Project No.:

Project:

92113411

J12030116

Sample: FGD PURGE EFF Lab ID: 92113411001 Collected: 03/04/12 09:24 Received: 03/06/12 11:11 Matrix: Water

Parameters Results Units Report Limit DF Prepared Analyzed CAS No. Qual

2540C Total Dissolved Solids Analytical Method: SM 2540C

Total Dissolved Solids 39700 mg/L 500 1 03/08/12 13:13

2540D Total Suspended Solids Analytical Method: SM 2540D

Total Suspended Solids 7100 mg/L 250 1 03/09/12 12:38

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 03/12/2012 04:36 PM Page 7 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 33 of 47
QUALITY CONTROL DATA

Pace Project No.:

Project:

92113411

J12030116

QC Batch:

QC Batch Method:

Analysis Method:

Analysis Description:

WET/19999

SM 2540C

SM 2540C

2540C Total Dissolved Solids

Associated Lab Samples: 92113411001

Parameter Units

Blank

Result

Reporting

Limit Qualifiers

METHOD BLANK: 731928

Associated Lab Samples: 92113411001

Matrix: Water

Analyzed

Total Dissolved Solids mg/L ND 25.0 03/08/12 13:01

Parameter Units

LCS

Result

% Rec

Conc. % Rec Limits Qualifiers

LABORATORY CONTROL SAMPLE: 731929

Spike LCS

Total Dissolved Solids mg/L 250 236 94 80-120

Parameter Units

Dup

Result Result RPD Qualifiers

92113392001

SAMPLE DUPLICATE: 731930

Total Dissolved Solids mg/L 7760 8200 6

Parameter Units

Dup

Result Result RPD Qualifiers

92113409001

SAMPLE DUPLICATE: 731931

Total Dissolved Solids mg/L 11100 9800 13 D6

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 03/12/2012 04:36 PM Page 8 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 34 of 47
QUALITY CONTROL DATA

Pace Project No.:

Project:

92113411

J12030116

QC Batch:

QC Batch Method:

Analysis Method:

Analysis Description:

WET/20019

SM 2540D

SM 2540D

2540D Total Suspended Solids

Associated Lab Samples: 92113411001

Parameter Units

Blank

Result

Reporting

Limit Qualifiers

METHOD BLANK: 732653

Associated Lab Samples: 92113411001

Matrix: Water

Analyzed

Total Suspended Solids mg/L ND 2.5 03/09/12 12:34

Parameter Units

LCS

Result

% Rec

Conc. % Rec Limits Qualifiers

LABORATORY CONTROL SAMPLE: 732654

Spike LCS

Total Suspended Solids mg/L 250 260 104 80-120

Parameter Units

Dup

Result Result RPD Qualifiers

92113272001

SAMPLE DUPLICATE: 732655

Total Suspended Solids mg/L 7.8 10 25 D6

Parameter Units

Dup

Result Result RPD Qualifiers

92113393003

SAMPLE DUPLICATE: 732656

Total Suspended Solids mg/L 28.0 28.2 1

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 03/12/2012 04:36 PM Page 9 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 35 of 47
QUALIFIERS

Pace Project No.:

Project:

92113411

J12030116

DEFINITIONS

DF - Dilution Factor, if reported, represents the factor applied to the reported data due to changes in sample preparation, dilution of

the sample aliquot, or moisture content.

ND - Not Detected at or above adjusted reporting limit.

J - Estimated concentration above the adjusted method detection limit and below the adjusted reporting limit.

MDL - Adjusted Method Detection Limit.

S - Surrogate

1,2-Diphenylhydrazine (8270 listed analyte) decomposes to Azobenzene.

Consistent with EPA guidelines, unrounded data are displayed and have been used to calculate % recovery and RPD values.

LCS(D) - Laboratory Control Sample (Duplicate)

MS(D) - Matrix Spike (Duplicate)

DUP - Sample Duplicate

RPD - Relative Percent Difference

NC - Not Calculable.

SG - Silica Gel - Clean-Up

U - Indicates the compound was analyzed for, but not detected.

N-Nitrosodiphenylamine decomposes and cannot be separated from Diphenylamine using Method 8270. The result reported for

each analyte is a combined concentration.

Acid preservation may not be appropriate for 2-Chloroethylvinyl ether, Styrene, and Vinyl chloride.

Pace Analytical is TNI accredited. Contact your Pace PM for the current list of accredited analytes.

LABORATORIES

PASI-A Pace Analytical Services - Asheville

ANALYTE QUALIFIERS

D6 The relative percent difference (RPD) between the sample and sample duplicate exceeded laboratory control limits.

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 03/12/2012 04:36 PM Page 10 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 36 of 47
QUALITY CONTROL DATA CROSS REFERENCE TABLE

Pace Project No.:

Project:

92113411

J12030116

Lab ID Sample ID QC Batch Method QC Batch Analytical Method

Analytical

Batch

92113411001 FGD PURGE EFF SM 2540C WET/19999

92113411001 FGD PURGE EFF SM 2540D WET/20019

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 03/12/2012 04:36 PM Page 11 of 11

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Analytical Lab 

Page 37 of 47
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

03/13/2012

Duke Energy Corporation (04)

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: Allen HAPS/MACT (Wastewater)

Project No.: J12030116

Lab Submittal Date: 03/06/2012

Prism Work Order: 2030114

VA Certification No. 1287

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 9

Analytical Lab 

Page 38 of 47
Sample Receipt Summary

03/13/2012

Prism Work Order: 2030114

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2012005462/FGD Purge Eff 2030114-01 Water 03/04/12 03/06/12

2012005464/BioReactor 1 Inf 2030114-02 Water 03/04/12 03/06/12

2012005466/BioReactor 2 Inf 2030114-03 Water 03/04/12 03/06/12

2012005468/BioReactor 2 Eff 2030114-04 Water 03/04/12 03/06/12

Samples received in good condition at 2.6 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 9

Analytical Lab 

Page 39 of 47
Laboratory Report

03/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Project: Allen HAPS/MACT 

(Wastewater)

Duke Energy Corporation (04)

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2030114-01

Prism Work Order: 2030114

Time Collected: 03/04/12 09:24

Time Submitted: 03/06/12 16:25

2012005462/FGD Purge Eff

J12030116

General Chemistry Parameters

pH 7.1 HT pH Units 1 *SM4500-H B 3/7/12 14:00 JAB P2C0135

Total Alkalinity 47 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0213

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0214

Bicarbonate Alkalinity 47 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0215

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 9

Analytical Lab 

Page 40 of 47
Laboratory Report

03/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Project: Allen HAPS/MACT 

(Wastewater)

Duke Energy Corporation (04)

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2030114-02

Prism Work Order: 2030114

Time Collected: 03/04/12 08:20

Time Submitted: 03/06/12 16:25

2012005464/BioReactor 1 Inf

J12030116

General Chemistry Parameters

pH 6.8 HT pH Units 1 *SM4500-H B 3/7/12 14:00 JAB P2C0135

Total Alkalinity 9.6 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0213

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0214

Bicarbonate Alkalinity 9.6 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0215

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 9

Analytical Lab 

Page 41 of 47
Laboratory Report

03/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Project: Allen HAPS/MACT 

(Wastewater)

Duke Energy Corporation (04)

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2030114-03

Prism Work Order: 2030114

Time Collected: 03/04/12 09:40

Time Submitted: 03/06/12 16:25

2012005466/BioReactor 2 Inf

J12030116

General Chemistry Parameters

pH 6.3 HT pH Units 1 *SM4500-H B 3/7/12 14:00 JAB P2C0135

Total Alkalinity 26 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0213

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0214

Bicarbonate Alkalinity 26 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0215

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 9

Analytical Lab 

Page 42 of 47
Laboratory Report

03/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Project: Allen HAPS/MACT 

(Wastewater)

Duke Energy Corporation (04)

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2030114-04

Prism Work Order: 2030114

Time Collected: 03/04/12 08:30

Time Submitted: 03/06/12 16:25

2012005468/BioReactor 2 Eff

J12030116

General Chemistry Parameters

pH 6.7 HT pH Units 1 *SM4500-H B 3/7/12 14:00 JAB P2C0135

Total Alkalinity 30 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0213

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0214

Bicarbonate Alkalinity 30 mg/L 5.0 0.66 1 *SM2320 B 3/12/12 12:45 JAB P2C0215

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 9

Analytical Lab 

Page 43 of 47
3/13/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12030116

Duke Energy Corporation (04) Project: Allen HAPS/MACT (Wastewater)

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2030114

Time Submitted: 3/6/2012 4:25:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2C0135 - NO PREP

LCS (P2C0135-BS1) Prepared & Analyzed: 03/07/12 

pH 6.91 pH Units 6.860 101 99-101

LCS (P2C0135-BS2) Prepared & Analyzed: 03/07/12 

pH 6.87 pH Units 6.860 100 99-101

Duplicate (P2C0135-DUP2) Source: 2030114-04 Prepared & Analyzed: 03/07/12 

pH 6.71 pH Units 6.70 0.1 10

Batch P2C0213 - NO PREP

Blank (P2C0213-BLK1) Prepared & Analyzed: 03/12/12 

Total Alkalinity BRL 5.0 mg/L

LCS (P2C0213-BS1) Prepared & Analyzed: 03/12/12 

Total Alkalinity 257 5.0 mg/L 250.0 103 90-110

LCS Dup (P2C0213-BSD1) Prepared & Analyzed: 03/12/12 

Total Alkalinity 258 5.0 mg/L 250.0 103 90-110 0.4 200

Batch P2C0214 - NO PREP

Blank (P2C0214-BLK1) Prepared & Analyzed: 03/12/12 

Carbonate Alkalinity BRL 5.0 mg/L

LCS (P2C0214-BS1) Prepared & Analyzed: 03/12/12 

Carbonate Alkalinity 257 5.0 mg/L 90-110

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 9

Analytical Lab 

Page 44 of 47
3/13/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12030116

Duke Energy Corporation (04) Project: Allen HAPS/MACT (Wastewater)

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2030114

Time Submitted: 3/6/2012 4:25:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2C0214 - NO PREP

LCS Dup (P2C0214-BSD1) Prepared & Analyzed: 03/12/12 

Carbonate Alkalinity 258 5.0 mg/L 90-110 0.4 200

Batch P2C0215 - NO PREP

Blank (P2C0215-BLK1) Prepared & Analyzed: 03/12/12 

Bicarbonate Alkalinity BRL 5.0 mg/L

LCS (P2C0215-BS1) Prepared & Analyzed: 03/12/12 

Bicarbonate Alkalinity 257 5.0 mg/L 250.0 103 90-110

LCS Dup (P2C0215-BSD1) Prepared & Analyzed: 03/12/12 

Bicarbonate Alkalinity 258 5.0 mg/L 250.0 103 90-110 0.4 200

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 8 of 9

Analytical Lab 

Page 45 of 47

Analytical Lab 

Page 47 of 47