# MDL: EPA Reference Methods MDL Lab 1 

# MDL Lab 2 

# MDL Lab 3 

# LIS 0.06 0.01 0.017 HIS 0.01 0.04 0.013 MDL 2 

# Lab 1 Lab 2 LIS 0.004 0.000 HIS 0.000 0.002 MDL pooled 

# (mg/L) EPA Reference Hach 10206 LIS 0.03 0.06 HIS 0.02 0.05 ML pooled 

# (mg/L) EPA Reference Hach 10206 LIS 0.09 0.20 HIS 0.06 0.17 Av erage % Recovery (X): EPA Reference Hach 10206 LIS 103.5% 99.0% HIS 103.8% 94.6% Between laboratory Std Dev (s b 

# ) EPA Reference Hach 10206 LIS 0.043 0.005 HIS 0.023 0.036 Lab 1 A vg Lab 2 A

vg Lab 3 A

vg EPA Reference LIS 108% 99% 104% HIS 106% 102% 104% Hach 10206 LIS 100% 99% 99% HIS 97% 90% 96% Pooled within-laboratory Std Dev (s w

# ) EPA Reference Hach 10206 LIS 0.030 0.006 HIS 0.016 0.005 Lab 1 Std Dev Lab 2 Std Dev Lab 3 Std Dev EPA Reference

# LIS 0.0068 0.0522 0.0027

HIS 0.0179 0.0207 0.0018 Hach 10206 LIS 0.0062 0.0077 0.0025 HIS 0.0077 0.0034 0.0023 Precision Acceptance Criteria F = √3.86 1.96468827 RSD EPA Reference Hach 10206 LIS 0.03 0.0 HIS 0.02 0.0 IPR QC @ 95% Conf Precision EPA Reference Hach 10206 LIS 0.06 0.01 Use HIS 0.03 0.01 Recovery Acceptance Criteria (s c 

# ) EPA Reference Hach 10206 LIS 0.05 0.01 HIS 0.03 0.04 (1+1/m) 1.333333333 (1/4-1/n) 0 QC Acceptance criteria (IPR) Recovery EPA Reference Hach 10206 Lower Limit (LIS) 88% 97% Lower Limit (HIS) 95% 81% Upper Limit (LIS) 119% 101% Upper Limit (HIS) 112% 108% Av erage Recovery
Matrix EPA Reference Lab 1 Lab 2 Lab 3 Av erage Percent Recovery 109 97.4 102 Standard Deviation 0.14 2.8 0.14 Overall mean recovery (X) 103 Between laboratory Std Dev (s b 

# ) 5.7 Pooled within-laboratory Std Dev (s w

# ) 1.6

# Hach 10206 Lab 1 Lab 2 Lab 3

Av erage Percent Recovery 96.0 94.3 95.1 Standard Deviation 0.28 0.42 1.7 Overall mean recovery (X) 95.1 Between laboratory Std Dev (s b 

# ) 0.85 Pooled within-laboratory Std Dev (s w

# ) 1.0 Recovery Acceptance Criteria (s c 

# ) EPA Reference Hach 10206 6.55 0.98 Lower limit (%) Recovery Range EPA Reference Hach 10206 Lower 85.7 92.6 Upper 120 97.7

# MDL: Hach Method 10206 MDL Lab 1 

# MDL Lab 2 

# MDL Lab 3 

# LIS 0.10 0.09 0.03 HIS 0.04 0.08 0.08 MDL 2 

# Lab 3 Lab 1 Lab 2 Lab 3

0.000 LIS 0.01 0.01 0.00 0.000 HIS 0.00 0.01 0.01

Std Dev squared Lab 1 Std Dev Lab 2 Std Dev Lab 3 Std Dev

# 0.0000 0.0027 0.0000 0.000925 0.0003 0.0004 0.0000 0.000252 0.0000 0.0001 0.0000 3.49E-05 0.0001 0.0000 0.0000 2.52E-05

Std Dev squared Lab 1 Std Dev Lab 2 Std Dev Lab 3 Std Dev Av erage 0.0200 8.0000 0.0200 2.6800

# Std Dev squared Lab 1 Std Dev Lab 2 Std Dev Lab 3 Std Dev 0.0800 0.1800 2.8800 1.0467

# Watertow n

EPA 353.2 MDL Study : 0.075 mg/L Spike

Av erage (mg/L) Std. Dev. MDL ML Rounded ML LIS 0.054 0.019 0.061 0.19 0.20 HIS 0.033 0.003 0.010 0.03 0.03 IPR Study : 5.0 mg /L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 Av g % Recovery LIS 107.6% 106.8% 107.2% 108.4% 107.5% HIS 107.4% 106.0% 107.4% 103.6% 106% Matrix Matrix (mg/L) MS(mg/L) MS D (mg/L) Av erage (mg/L) Av g Recovery -0.03 5.43 5.44 5.435 108.7%

108.6 108.8 109 Hach M ethod 10206 MDL Study : 0.3 mg/L Spike

Av erage (mg/L) Std. Dev. MDL ML Rounded ML LIS 0.286 0.030 0.10 0.30 0.30 HIS 0.208 0.011 0.04 0.11 0.10 IPR Study : 5.0 mg /L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 Av g % Recovery LIS 100.4% 99.4% 99.2% 99.0% 99.5% HIS 97.6% 97.6% 96.8% 96.0% 97.0% Matrix Matrix (mg/L) MS(mg/L) MS D (mg/L) Av erage (mg/L) Av g Recovery -0.08 4.81 4.79 4.80 96.0%

96.2 95.8 96.0

# Std. Dev.

0.01 0.02 Standard Deviation %RSD 0.14 0.13

Std. Dev. 0.006 0.008 Standard Deviation %RSD 0.28 0.29

# Cedar Falls

SM 20th Ed. 4500-NO3 E MDL Study : 0.075 mg/L Spike

Av erage (mg/L) Std. Dev. MDL ML Rounded ML LIS 0.072 0.002 0.01 0.02 0.02 HIS 0.079 0.013 0.04 0.13 0.10 IPR Study : 5.0 mg /L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 Av g % Recovery LIS 91.2% 101.5% 101.7% 101.7% 99.0% HIS 98.6% 101.5% 103.0% 103.0% 102% Matrix Matrix (mg/L) MS(mg/L) MS D (mg/L) Av erage (mg/L) Av g Recovery 1.41 6.18 6.38 6.28 98.0%

95.4 99.4 97.4 Hach M ethod 10206 MDL Study : 0.3 mg/L Spike

Av erage (mg/L) Std. Dev. MDL ML Rounded ML LIS 0.303 0.030 0.09 0.295 0.30 HIS 0.294 0.024 0.08 0.239 0.20 IPR Study : 5.0 mg /L Spike

% Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 Av g % Recovery LIS 99.6% 98.6% 98.2% 99.8% 99.1% HIS 90.4% 90.0% 90.8% 90.6% 90.5% Matrix Matrix (mg/L) MS(mg/L) MS D (mg/L) Av erage (mg/L) Av g Recovery 2.00 6.73 6.70 6.72 95.9%

94.6 94 94.3

# Std. Dev.

0.05 0.02 Stdev %RSD 2.8 2.9

Std. Dev. 0.008 0.003 Stdev %RSD 0.42 0.45

# Phoenix

EPA 300.0 MDL Study : 0.6 mg/L Spike Av erage (mg/L) Std. Dev. MDL ML LIS 0.582 0.005 0.017 0.054 HIS 0.583 0.004 0.013 0.042 IPR Study : 5.0 mg /L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 104.2% 104.0% 103.9% 103.5% HIS 104.0% 103.9% 103.6% 103.7% Matrix Matrix (mg/L) MS(mg/L) MS D (mg/L) Av erage (mg/L) 0.07 5.18 5.17 5.18

102.2 102 Hach M ethod 10206 MDL Study : 0.3 mg/L Spike Av erage (mg/L) Std. Dev. MDL ML LIS 0.245 0.011 0.03 0.110 HIS 0.251 0.024 0.08 0.239 IPR Study : 5.0 mg /L Spike % Recovery #1 % Recovery #2 % Recovery #3 % Recovery #4 LIS 98.8% 98.6% 98.6% 98.2% HIS 96.6% 96.2% 96.2% 96.6% Matrix Matrix (mg/L) MS(mg/L) MS D (mg/L) Av erage (mg/L) 0.307 5.12 5.00 5.06

96.3 93.9

# Rounded ML 0.10 0.10 Av g % Recovery Std. Dev.

103.9% 0.003 103.8% 0.002 Av g Recovery Stdev RSD

102.1% 0.14 13.9 102.1 0.137255
Rounded ML 0.10

0.20 Av g % Recovery Std. Dev.

98.6% 0.003 96.4% 0.002 Av g Recovery Stdev RSD

95.3% 1.7 1.79 95.1Analyst Name: Facility Name:

City, State: Date: Drinking W ater Source: EPA Reference Method: 

### Calibration Verification 0.30 mg/L NO 3 - -N standard solution

Hach Method 10206 (mg/L NO 3 --N) 10.0 mg/L NO 3 - -N standard solution

Hach Method 10206 (mg/L NO 3 --N) 

### MDL - Low Ionic Strength: Hach Method 10206 Expected Conc. 0.30 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N) Replicate 5 (Abs) Replicate 6 (Abs) Replicate 7 (Abs)


### MDL - Low Ionic Strength: EPA Reference Method Expected Conc. 0.075 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N)



## Datasheet for the Validation of TNTplusTM Nitrate Reagent in Drinking Water

Note: This sheet must be printed off and filled out in hard copy form. Upon completion, please also fill out the electronic copy and submit both back to Hach Company. Yellow boxes must be filled out by the analyst. A calibration report for the reference method must accompany this data sheet.
TestAmerica Grant Johnson 10/25/2010

0.288 0.096 Watertown, W I

9.69 0.285 WT I0197-02 Lab W ater 353.2

0.101 0.106 0.097

0.050 0.1

0.097 0.036 0.048 0.284

0.050 0.051 0.049 0.260 0.299 0.345 0.264

0.260 0.096 0.099

### MDL - High Ionic Strength: Hach Method 10206 Expected Conc. 0.30 (mg/L NO 3 --N)

Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N) Replicate 5 (Abs) Replicate 6 (Abs) Replicate 7 (Abs) 

### MDL - High Ionic Strength: EPA Reference Method Expected Conc. 0.075 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N)



### IPR - Low Ionic Strength: Hach Method 10206 Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs)


### IPR - Low Ionic Strength: EPA Reference Method Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) 0.089 0.09 0.091 0.088 0.195 0.206 0.215 0.190

0.031 0.040 0.032 0.033 0.216 0.220 0.213 0.091 0.092 0.091

0.032 0.031 0.031

5.02 4.97 4.96

5.38 5.34 5.36 5.42 4.95 0.641 0.636 0.634 0.633

### IPR - High Ionic Strength: Hach Method 10206 Expected Conc. 5.0 (mg/L NO 3 --N)

Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) 

### IPR - High Ionic Strength: EPA Reference Method Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N)



### Drink ing Water Sample Matrix Hach Method 10206 (mg/L NO 3 --N) (Abs) EPA Reference Method (mg/L NO 3 --N)


### Drink ing Water Sample Matrix Spik e Hach Method 10206 (mg/L NO 3 --N) (mg/L NO 3 --N) Replicate 1 Replicate 2 (Abs) (Abs) Replicate 1 Replicate 2 EPA Reference Method (mg/L NO 3 --N) (mg/L NO 3 --N) Replicate 1 Replicate 2 

### Drink ing Water Matrix Characterization Total Chlorine: (mg/L Cl 2 ) Total Hardness: (mg/L CaCO 3 ) Total Alkalinity: (mg/L CaCO 3 ) pH: 5.37 5.18 4.80 0.625 0.625 0.621 0.616 4.88 4.88 4.84

280 7.35 5.37 5.30

-0.08

-0.03

4.81

5.43 0.06

4.79

5.44

0.055 <8.2 0.617 0.615Date:

0.30

0.075

0.0717 0.0727 0.0711 Replicate 5 (mg/L NO Replicate 6 (mg/L NO 3 Replicate 7 (mg/L NO 3 Replicate 1 (mg/L NO Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 Replicate 4 (mg/L NO 3 0.0692 0.0718 0.0743 0.0737 

### MDL - Low Ionic Strength: EPA Reference Method Expected Conc. (mg/L NO 3 --N) Replicate 5 (Abs) Replicate 6 (Abs) Replicate 7 (Abs) Replicate 5 (mg/L NO Replicate 6 (mg/L NO 3 Replicate 7 (mg/L NO 3 0.099 0.104 0.102 0.284 0.324 0.313 Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) 0.103 0.106 0.097 0.097 Replicate 1 (mg/L NO Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 Replicate 4 (mg/L NO 3 0.322 0.341 0.269 0.266 

### MDL - Low Ionic Strength: Hach Method 10206 Expected Conc. (mg/L NO 3 --N) 10.0 mg/L NO 3 - -N standard solution Hach Method 10206 9.99 (mg/L NO 3 --N) Hach Method 10206 0.285 (mg/L NO 3 --N) 

### Calibration Verification 0.30 mg/L NO 3 - -N standard solution Drinking W ater Source: Cedar Falls EPA Reference Method: SM 20th Ed. 4500-NO 3E City, State: Cedar Falls, Iowa 9/29/2010 Analyst Name: Lorna Bormann Facility Name: TestAmerica Cedar Falls A calibration report for the reference method must accompany this data sheet. 

## Datasheet for the Validation of TNTplusTM Nitrate Reagent in Drinking Water

Note: This sheet must be printed off and filled out in hard copy form. Upon completion, please also fill out the electronic copy and submit both back to Hach Company. Yellow boxes must be filled out by the analyst.0.30

0.075

5.0

5.0

5.0

5.0 Replicate 1 (mg/L NO Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 Replicate 4 (mg/L NO 3 4.93 5.075 5.15 5.15 Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) 

### IPR - High Ionic Strength: EPA Reference Method Expected Conc. (mg/L NO 3 --N) 0.584 0.582 0.586 0.586 Replicate 4 (mg/L NO 3 Replicate 1 (mg/L NO Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 

### IPR - High Ionic Strength: Hach Method 10206 Expected Conc. (mg/L NO 3 --N) 4.52 4.5 4.54 4.53 Replicate 1 (mg/L NO Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 Replicate 4 (mg/L NO 3 4.56 5.075 5.084 5.086 

### IPR - Low Ionic Strength: EPA Reference Method Expected Conc. (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 0.636 0.63 0.629 0.638 Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) Replicate 4 (mg/L NO 3 4.98 4.93 4.91 4.99 Replicate 1 (mg/L NO 

### IPR - Low Ionic Strength: Hach Method 10206 Expected Conc. (mg/L NO 3 --N) 0.0889 0.0922 0.071 Replicate 5 (mg/L NO Replicate 6 (mg/L NO 3 Replicate 7 (mg/L NO 3 Replicate 1 (mg/L NO Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 Replicate 4 (mg/L NO 3 0.0625 0.0639 0.0887 0.0841 

### MDL - High Ionic Strength: EPA Reference Method Expected Conc. (mg/L NO 3 --N) 0.096 0.098 0.102 Replicate 5 (Abs) Replicate 6 (Abs) Replicate 7 (Abs) 0.254 0.272 0.307 Replicate 5 (mg/L NO Replicate 6 (mg/L NO 3 Replicate 7 (mg/L NO 3 Replicate 2 (mg/L NO 3 Replicate 3 (mg/L NO 3 0.101 0.1 0.104 0.101 Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) Replicate 4 (mg/L NO 3 0.302 0.295 0.326 0.304 Replicate 1 (mg/L NO 

### MDL - High Ionic Strength: Hach Method 10206 Expected Conc. (mg/L NO 3 --N)(Abs)

(Abs) (Abs)

pH: 7.4 Total Alkalinity: 236.9 (mg/L CaCO 3 ) 284.2 (mg/L CaCO 3 ) Total Chlorine: 0.23 (mg/L Cl 2 ) Total Hardness: Replicate 1 Replicate 2 

### Drinking Water Matrix Characterization EPA Reference Method 6.18 (mg/L NO 3 --N) (mg/L NO 3 --N) Replicate 2 0.84 0.83
6.38 Replicate 1 Hach Method 10206 6.73 (mg/L NO 3 --N) Replicate 1 Replicate 2 6.70 

### Drinking Water Sample Matrix Spike (mg/L NO 3 --N) (mg/L NO 3 --N) (mg/L NO 3 --N) 0.296 EPA Reference Method 1.41 

### Drinking Water Sample Matrix Hach Method 10206 2.00Analyst Name: Facility Name:

City, State: Date: Drinking W ater Source: EPA Reference Method: 

### Calibration Verification 0.30 mg/L NO 3 - -N standard solution

Hach Method 10206 (mg/L NO 3 --N) 10.0 mg/L NO 3 - -N standard solution

Hach Method 10206 (mg/L NO 3 --N) 

### MDL - Low Ionic Strength: Hach Method 10206 Expected Conc. 0.30 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N) Replicate 5 (Abs) Replicate 6 (Abs) Replicate 7 (Abs)


### MDL - Low Ionic Strength: EPA Reference Method Expected Conc. 0.60 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N) 0.576
0.576 0.586 0.586 0.096

0.094 0.094 0.094

0.577 0.585 0.588 0.244 0.244 0.239 City of Phoenix

0.092 0.096 0.095 300.0 - Ion Chromatography

0.257
9.980

0.225 0.255 0.246 0.259 Phoenix, Arizona 9-14 through 9-24-2010 

## Datasheet for the Validation of TNTplusTM Nitrate Reagent in Drinking Water

Note: This sheet must be printed off and filled out in hard copy form. Upon completion, please also fill out the electronic copy and submit both back to Hach Company. Yellow boxes must be filled out by the analyst. A calibration report for the reference method must accompany this data sheet.
Kurt ILL TestAmerica - Phoenix

### MDL - High Ionic Strength: Hach Method 10206 Expected Conc. 0.30 (mg/L NO 3 --N)

Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N) Replicate 5 (Abs) Replicate 6 (Abs) Replicate 7 (Abs) 

### MDL - High Ionic Strength: EPA Reference Method Expected Conc. 0.6 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 5 (mg/L NO 3 --N) Replicate 6 (mg/L NO 3 --N) Replicate 7 (mg/L NO 3 --N)



### IPR - Low Ionic Strength: Hach Method 10206 Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs)


### IPR - Low Ionic Strength: EPA Reference Method Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) 

### IPR - High Ionic Strength: Hach Method 10206 Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N) Replicate 1 (Abs) Replicate 2 (Abs) Replicate 3 (Abs) Replicate 4 (Abs) 

### IPR - High Ionic Strength: EPA Reference Method Expected Conc. 5.0 (mg/L NO 3 --N) Replicate 1 (mg/L NO 3 --N) Replicate 2 (mg/L NO 3 --N) Replicate 3 (mg/L NO 3 --N) Replicate 4 (mg/L NO 3 --N)

5.2 5.197 5.181 5.187 4.83 4.81 4.81 4.83 0.62 0.618 0.617 0.619 0.632 0.631 0.631 0.629

5.21 5.199 5.196 5.177 0.585
0.574 0.586 0.585

4.94 4.93 4.93 4.91 0.096 0.094 0.094

0.585 0.582 0.581 0.093 0.095 0.094 0.101 0.254 0.239 0.24 0.233 0.246 0.241 0.303

### Drinking Water Sample Matrix

Hach Method 10206 (mg/L NO 3 --N) (Abs) EPA Reference Method (mg/L NO 3 --N) 

### Drinking Water Sample Matrix Spike Hach Method 10206 (mg/L NO 3 --N) (mg/L NO 3 --N) Replicate 1 Replicate 2 (Abs) (Abs) Replicate 1 Replicate 2 EPA Reference Method (mg/L NO 3 --N) (mg/L NO 3 --N) Replicate 1 Replicate 2 

### Drinking Water Matrix Characterization Total Chlorine: (mg/L Cl 2 ) Total Hardness: (mg/L CaCO 3 ) Total Alkalinity: (mg/L CaCO 3 ) pH: 184 80.79 8.11 5.00

0.653 0.638

0.362 5.18 5.17

0.307 0.102 0.07

5.12