UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON, D.C. 20460 

Ms. Stephanie Burton 

Biotech Regulatory Manager 

Dow AgroSciences LLC 

9330 Zionsville Road 

Indianapolis, IN 46268-1054 

MAR 0 4 2016 OFFICE OF CHEMICAL SAFETY 

AND POLLUTION PREVENTION 

Re: Experimental Use Permit Amendment and Extension for Com Plant-incorporated Protectants 

(PIPs) with MON 87411 (DvSnf7 dsRNA and Bacillus thuringiensis (Bt) Cry3Bbl protein), MON 

89034 (Bt CrylA.105 and Bt Cry2Ab2 proteins), MON 88017 (Bt Cry3Bbl protein), TC1507 (Bt 

CrylF protein), and/or DAS-59122-7 (Bt Cry34Abl and Bt Cry35Abl proteins) 

EPA Experimental Use Permit No.: 62719-EUP-66 

Decision No.: 511429 

Application Dated: November 23, 2015 

Effective Dates: Immediately through March 31, 2017 (includes planting and associated activities, 

e.g., collection of field data, harvesting, and processing) 

Quantity Authorized: 8.509 pounds (3,860 grams) of active ingredient (0.399, 0.120, 9.364 x 10-1, 

0.448, 6.22, 0.152, and 1.17 pounds of Bt Cry 1A.I05 protein, Bt Cry2Ab2 protein, DvSnf7 

dsRNA, Bt CrylF protein, Bt Cry34Abl protein, Bt Cry35Abl protein, and Bt Cry3Bbl 

protein, respectively) in 456,699 pounds of seed 

Acres: 5,844 acres, including PIP (unregistered = 965; registered = 1,695; total= 2,660), non-PIP 

(1 ,312), and border (1,872) acreage 

Dear Ms. Burton: 

On the basis of the information furnished by you, the U.S. Environmental Protection Agency (EPA) 

hereby amends and extends the subject Experimental Use Permit (EUP) under section 5 of the Federal 

Insecticide, Fungicide, and Rodenticide Act (FIFRA) to test MON 87411 (DvSnf7 dsRNA and Bacillus 

thuringiensis (Bt) Cry3Bbl protein) alone or in combination with the following plant-incorporated 

protectants (PIPs): MON 89034 (Bt CrylA.105 and Bt Cry2Ab2 proteins), MON 88017 (Bt Cry3Bbl 

protein), TC1507 (Bt CrylF protein), and/or DAS-59122-7 (Bt Cry34Abl and Bt Cry35Abl proteins). 

This EUP is authorized only in the Commonwealth of Puerto Rico (PR) and in the States of Arkansas 

(AR), California (CA), Georgia (GA), Hawaii (HI), Illinois (IL), Indiana (IN), Iowa (IA), Kansas (KS), 

Minnesota (MN), Missouri (MO), Nebraska (NE), North Carolina (NC), North Dakota (ND), Ohio 

(OH), Oklahoma (OK), Pennsylvania (PA), South Carolina (SC), South Dakota (SD), Texas (TX), and 

Wisconsin (WI) for inbred and hybrid development, nursery observations, and testing and collection of 

product characterization data. 
EPA EUP No. 62719-EUP-66 

Decision No. 511429 

Shipment and/or use under this permit are subject to the provisions of 40 CFR Part 172. The Bt proteins 

to be used in this EUP include CrylA.105, Cry2Ab2, CrylF, Cry3Bbl , Cry34Abl , and Cry35Abl. The 

EPA previously determined the environmental and human health safety of these proteins and exempted 

them from the requirement of a tolerance as follows: 40 CFR §§ l 74.502(a) (Bt CrylA.105 protein), 

174.504 (Bt Cryl F protein), 174.506 (Bt Cry34Abl and Bt Cry35Abl proteins), 174.518 (Bt Cry3Bbl 

protein), and l 74.5 l 9(a) (Bt Cry2Ab2 protein). Jhe ~p AJws also established a permanent tolerance 

exemption for nucleic acids; this includes n$sn}7 dsRNA that is expressed in MON 87411 (40 CFR § 

174.507). The marker proteins phosphinothricin acetyltransferase (PAT), CP4 enolpyruvylshikimate-3-

phosphate synthase (CP4 EPSPS), and phosphomannose isomerase (PMI) are also exempt from the 

requirement of a tolerance at 40 CFR §§ 174.522 and 174.523, respectively. Harvested plant material 

from PIPs that have not yet been commercially registered must not be allowed to enter the food or 

feed supply. 

Prior to shipment and/or use of the subject com, you must consult with the pesticide regulatory officials 

and, if applicable, the biotechnology regulatory officials of the states 1 in which you will conduct your 

experimental program, and obtain a state permit or license if such is required. Amendment and extension 

of this Federal permit does not negate the need for permission from individual states. Prior to initiating 

this experimental program in any state, you are required to notify the lead agency of the states in which 

you will conduct your experimental program of the specific testing program (when, where, how much, 

etc.). Failure to do so may result in revocation or modification of this EUP. You must comply with all 

requirements of the U.S. Department of Agriculture's Animal and Plant Health Inspection Service as 

they apply to the proposed testing. 

You must provide notice to the state pesticide regulatory agency at least 72 hours before you plant the 

experimental use PIPs. You must also provide a copy of this authorization letter, the stamped, accepted 

EUP label, and a non-confidential portion of the Section G Experimental Program to all cooperators, 

participants, and users prior to initial use of the experimental use PIPs in accordance with this EUP. The 

EPA will notify the relevant EPA Regions of the approval of this EUP by sending copies of this 

authorization letter to them. 

Conditions under which the EPA is amending and extending this EUP are as follows: 

I . The acres, amount of pesticide, and amount of seed to be used shall not exceed the quantity 

authorized by this EUP. See Tables 1, 2, 3, 4, and 5 for specific details. 

T a bl e 1 S . ummary o f th . e A creae:e amone: PLP N PLP d B d Pl f ' on- , an or er an me:s 

State Unregistered Registered Total 

PIP PIP PIP Non-PIP Border Overall State Total 

AR 42 78 120 50 100 270 

CA 8 20 28 50 20 98 

GA 42 78 120 50 100 270 

HI 84 111 195 130 40 365 

IL 93 152 245 90 210 545 

IN 112 173 285 106 226 617 

1 For the purpose of this letter, the terms "state," "states," and "state-by-state" include the Commonwealth of Pueno Rico. 

2 
EPA EUP No. 62719-EUP-66 

Decision No. 511429 

State Unregistered Registered 

PIP PIP 

IA 104 168 

KS 8 20 

MN 67 101 

MO 8 20 

NE 64 111 

NC 8 20 

ND 30 68 

OH 36 68 

OK 8 20 

PA 8 20 

PR 75 155 

SC 42 78 

SD 46 80 

TX 38 76 

WI 42 78 

TOTAL: 965 1,695 

Total 

PIP Non-PIP Border Overall State Total 

272 86 206 564 

28 50 20 98 

168 60 110 338 

28 50 20 98 

175 60 110 345 

28 50 20 98 

98 50 100 248 

104 50 100 254 

28 50 20 98 

28 50 20 98 

230 80 50 360 

120 50 100 270 

126 50 100 276 

114 50 100 264 

120 50 100 270 

2,660 1,312 1,872 5,844 

3 
EPA EUP No. 627 19-EUP-66 

Decision No. 511429 

Table 2. Acreae:e Distribution between Experimental Protocols (Pl and P2) 

Pl 

State Registered Unregistered Total 

Pl Registered 

AR 0 0 0 78 

CA 0 0 0 20 

GA 0 0 0 78 

HI 75 50 125 36 

IL 30 17 47 122 

IN 40 29 69 133 

IA 40 29 69 128 

KS 0 0 0 20 

MN 15 II 26 86 

MO 0 0 0 20 

NE 15 II 26 96 

NC 0 0 0 20 

ND 0 0 0 68 

OH 0 0 0 68 

OK 0 0 0 20 

PA 0 0 0 20 

PR 75 35 110 80 

SC 0 0 0 78 

SD 0 0 0 80 

TX 0 0 0 76 

WI 0 0 0 78 

TOTAL: 290 182 472 1,405 

Notes: Pl = Protocol I (breeding and associated data) 

P2 = Protocol 2 (characterization) 

P2 TOTAL (Pl+P2) 

Total Unregistered P2 Registered Unregistered 

42 120 78 42 

8 28 20 8 

42 120 78 42 

34 70 111 84 

76 198 152 93 

83 216 173 11 2 

75 203 168 !04 

8 28 20 8 

56 142 IOI 67 

8 28 20 8 

53 149 111 64 

8 28 20 8 

30 98 68 30 

36 104 68 36 

8 28 20 8 

8 28 20 8 

40 120 155 75 

42 120 78 42 

46 126 80 46 

38 114 76 38 

42 120 78 42 

783 2,188 1,695 965 

4 

Total 

Pl+P2 

120 

28 

120 

195 

245 

285 

272 

28 

168 

28 

175 

28 

98 

104 

28 

28 

230 

120 

126 

114 

120 

2,660 
EPA EUP No. 62719-EUP-66 

Decision No. 511429 

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x r--

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UnmPstered PIPs 

x - .... x - .... ......... ~o!;; 

00 !!:.! z a~o ::E ::E 

x I";" - x N 

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x x r;-

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x x r--

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'3 

{:. 

.:I 

c; 

Z! 

c 

AR P l 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 O 

P2 4 4 4 10 4 6 4 6 4 6 4 6 4 6 6 78 6 10 6 4 4 4 8 42 120 

Pl+P2 4 4 4 10 4 6 4 6 4 6 4 6 4 6 6 78 6 10 6 4 4 4 8 42 120 

CA Pl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 

P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

P l+P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

GA P l 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 

P2 4 4 4 10 4 6 4 6 4 6 4 6 4 6 6 78 6 10 6 4 4 4 8 42 120 • 

P l+P2 4 4 4 10 4 6 4 6 4 6 4 6 4 6 6 78 6 10 6 4 4 4 8 42 120 

HIPIH 

P2 3 

Pl+P2 8 

5 5 I 5 I 5 5 5 5 5 5 5 5 5 5 5 75 I 5 20 5 5 5 5 5 50 I 125 

I I I I 4 

6 6 I 6 I 9 

3 

8 

II 

16 6 

2 

7 6 

2 

7 

3 

8 6 6 6 

36 I I 

111 I 6 

28 

48 6 6 6 6 6 

34 I 70 

84 I 195 

IA Pl 2 2 2 4 2 2 2 10 2 2 2 2 2 2 2 40 4 15 2 2 2 2 2 29 69 I 

P2 8 6 8 20 8 8 12 8 6 8 6 8 6 8 8 128 8 25 8 6 8 8 12 75 203 I 

Pl+P2 10 8 10 24 10 10 14 18 8 10 8 10 8 10 10 168 12 40 10 8 10 10 14 • 104 272 

ILPIH 

P2 6 

P l+P2 8 

IN P l I 2 

P2 ls 

2 2 I 2 I 2 

6 6 I 20 I 6 

8 8 I 22 I 8 

2 2 I 4 I 2 

6 8 I 25 I 8 

2 2 2 

8 12 8 

10 14 10 

2 2 10 

8 12 8 

2 2 2 2 2 

6 8 6 8 6 

8 10 8 10 8 

2 2 2 2 2 

6 8 6 8 6 

5 

2 2 30 I 2 5 2 2 2 2 2 17 I 47 

8 8 122 I 6 25 8 6 8 8 15 76 I 198 

10 10 152 I 8 30 10 8 10 10 17 93 I 245 

2 2 40 I 4 15 2 2 2 2 2 29 I 69 

8 8 133 I 8 35 8 6 8 8 10 83 I 2 16 
EPA EUP No. 62719-EUP-66 

Decision No. 511429 

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x 

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Registered PlPs 

x x ..,. .... ,.... _ ,... 000 °' 0000 00 "' - z ..,.. u 

0 0 !-

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x x r;-

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x ~ 

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x ~ = i::i ,..... ,...... V -

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I 73 I 12 I 50 

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Unregistered PIPs 

x - ;'3; x ~ or--.--

0\0oo 

00 ~ z 

zuo Ot- ;:E :E 

8 

x ~ 

- xN - N ..,. .... _ t"'- 0 °' 00 "' "' 

6~~ :E 0 

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x x r;-

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66~ ;:E ;:EO 

10 

i ~ ..,. .... x _ , -.!! .... 0 °' 0 00"""" :c 

~~~~ ~ 

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(,.') 

12 112 I 285 

KS Pl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 

P2 2 I 2 2 2 . 2 I I I I I I I I I 20 I I I I I I 2 8 28 

Pl+P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

MNPI~ 

P2 4 

Pl+P2 5 

I I I 

6 4 6 6 

5 

11 I 26 

4 

5 

4 10 I 4 12 6 4 6 4 6 

7 13 5 7 7 7 

15 I I 5 

8 

9 

56 I 142 

11 I 5 7 5 5 67 I 168 

86 I 6 20 6 6 6 

5 

4 

7 IOI I 7 25 7 7 7 

MO Pl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

P l+P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

NC Pl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

Pl+P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

ND Pl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 

P2 4 4 4 I 0 4 4 4 6 4 4 4 4 4 4 4 68 4 4 4 4 4 4 6 30 98 I 

Pl+P2 4 4 4 10 4 4 4 6 4 4 4 4 4 4 4 68 4 4 4 4 4 4 6 30 98 

NE Pl I I I I I I I I I I I I I I I 15 I 5 I I I I I 11 26 I 

P2 6 4 6 12 6 6 I 0 6 4 6 4 6 4 6 I 0 96 6 15 6 4 6 6 I 0 53 149 I 

Pl +P2 7 5 7 13 7 7 11 7 5 7 5 7 5 7 II 11 1 7 20 7 5 7 7 11 64 175 

OH Pl I 0 0 0 o I o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 o I o 

6 
EPA EUP No. 627 19-EUP-66 

Decision No. 511429 

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Registered PlPs 

x x 

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x x r;-

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x x ,..... x 

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4 IO 

4 IO 

x - v-

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Zo 

~~ 

4 

4 

Unregistered PIPs 

x - v x - ......... v ~ 0 l;; oo~z 

zuo 

Ob~ 

~ 

4 

4 

x r;- - x N 

~s~ 

~~~ u~ ~bo 

4 

4 

x x r;-

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55~ ~~o 

4 

4 

x x ..... 

8 ~ ~ 1 i ~~~~ ~ 

55~~ ~ ~~ 0 

"C 

~ c 

Z! 

!;;) 

6 36 I I04 

6 36 I I04 

OK P l 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 

P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 1 

Pl +P2 2 I 2 2 2 2 I I I I I I I I I 20 I I I I I I 2 8 28 

PAPIH 

P2 2 

Pl+P2 2 

0 0 o I o 

2 I 2 

o I o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

2 2 I 2 2 

2 

0 

20 

20 

2 

2 

8 I 28 

2 8 I 28 

PR Pl I 5 I 5 I 5 I 5 I 5 I 5 5 5 5 5 5 5 5 5 5 75 5 5 5 5 5 5 5 35 110 I 

P2 I 5 I 5 I 5 I 5 I 5 I 5 I 0 5 5 5 5 5 5 5 5 80 5 I 0 5 5 5 5 5 40 120 

Pl+P21 IO I IO I IO I IO I IO IO I5 10 10 IO IO 10 IO IO 10 I55 10 15 10 10 10 10 10 75 230 

SC Pl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 

P2 4 4 4 IO 4 6 4 6 4 6 4 6 4 6 6 78 6 IO 6 4 4 4 8 42 120 

Pl +P2 4 4 4 10 4 6 4 6 4 6 4 6 4 6 6 78 6 10 6 4 4 4 8 42 I20 

SD P I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 

P2 4 4 4 I 0 4 6 6 6 4 6 4 6 4 6 6 80 6 I 0 6 4 6 6 8 46 126 I 

P l+P2 4 4 4 10 4 6 6 6 4 6 4 6 4 6 6 80 6 10 6 4 6 6 8 46 126 

TXP I~ 

P216 

P I+P21 6 

0 0 

4 6 

4 6 

o I o 0 0 0 

10 I 6 6 4 6 

IO I 6 6 4 6 

0 0 0 0 0 

4 4 4 4 4 

4 4 4 4 4 

7 

0 0 0 0 0 0 0 0 0 0 o I o 

4 4 76 4 10 4 4 4 4 8 38 I 114 

4 4 76 4 10 4 4 4 4 8 38 I I 14 
EPA EUP No. 62719-EUP-66 

Decision No. 511429 

~ 

~ 

WIPlk 

P2 4 

P l+P2 4 

,_ 

0 

00 

00 

~ 

0 

4 

4 

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0 

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4 

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00 

~ 

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0 0 

10 4 

10 4 

Pl I 18 I 18 I 18 I 22 I 18 

r;- x ~ ,_ Oo--

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0 

6 

6 

18 

P2 86 70 84 185 87 100 

P l+ 104 88 102 207 105 118 

x ,_ ..., _ MO 

~::g 

ooz 50 

~~ 

0 

4 

4 

18 

11 9 

137 

x 

~ ,_ ~ 5:: oo6~ ~ 

0 

6 

6 

34 

96 

130 

Registered PIPs 

x x ..., ,_ .., _ ,_ 000 °' 0000 00 "' 

-

66~ ~~ 

0 

4 

4 

18 

7 1 

89 

x x f7' 

~!:::~ 00 -

°' 00 00 00 °' "' 

z5~ 

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0 

6 

6 

18 

90 

108 

Notes: PI = Protocol I (breeding and associated data) 

P2 = Protocol 2 (characterization) 

x ";" ..., X N 

8s~ 

~~~ Z U en OE-< 

~ 0 

0 

4 

4 

18 

71 

89 

x x r-;--

~ !::: x ~ 

~~So: 00 00 Vl "' 

66R~ ~~ 0 

0 

6 

6 

x 

,_ 

- ,_ 00 

00"' zu oi-

~ 

0 

4 

4 

x ,_ ,_ r:i - N 0 -

00 00 °' ";' 

z~ 

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0 

6 

6 

TOTAL Pl + P2 

18 

92 

11 0 

8 

18 

70 

88 

18 

90 

108 

x ";" 

!::: x ~ 

~b;; 

00 "' "' z u ch ~!-C§ 

0 

6 

6 

18 

'B 

s 

.&> 

<I) = 

0 

78 

78 

x ,_ 

... x ,_..., ~ = ~ _ ~ 00 ~ ~ 

u6 6~ !- ~ ~ 0 

0 0 

6 10 

6 10 

290 I 22 I 70 

..., x -_ 

~~ ooz 50 

~~ 

0 

6 

6 

18 

94 1,405 88 238 90 

112 1,695 110 308 108 

Unregistered PIPs 

x - ..., x -

...... "f!: ~ 0 00 

00 ~ z 

zuo 

OE-~ 

~ 

0 

4 

4 

18 

70 

88 

x r;- - x N :;;: ,_ !::! r-O °' 00 "' "' 

5R~ ~ 0 

0 

4 

4 

18 

82 

100 

x x r-;-

O• ~ =~ - °' 00 00"' ,_ °' z5~ 

~~Cl 

0 

4 

4 

18 

82 

100 

~ ~ x , - ~~s!::! ~ 0000~~ .c 

66~~ ~ 

'B 

~ '1:1 

c 

f 

~ 

~~ 0 

0 

8 

8 

18 

133 

151 

o I o 

42 I 120 

42 I 120 

182 I 472 

783 2,188 

965 2,660 
EPA EUP No. 627 19-EUP-66 

Decision No. 511 429 

Table 4. Total Pounds o rs eed E xpected to b e U se db P . /RN •Y rot em A an d S tate (f or Pl + P2 A crea2e 

State CrylA.105 Cry2Ab2 DvSnj7 Cryl F 

AR 2,632 2,632 2,444 2,9 14 

CA 6 11 6 11 470 705 

GA 2,632 2,632 2,444 2,9 14 

HI 3,854 3,854 4,230 3,666 

IL 5,3 11 5,3 11 5,405 5,405 

IN 5,546 5,546 6,627 5,828 

IA 5,640 5,640 6,0 16 5,922 

KS 6 11 6 11 470 705 

MN 3,6 19 3,619 3,666 3,572 

MO 6 11 6 11 470 705 

NE 3,713 3,713 3,6 19 3,948 

NC 6 11 6 11 470 705 

ND 2,256 2,256 1,880 2,444 

OH 2,256 2,256 2, 162 2,444 

OK 6 11 6 11 470 705 

PA 6 11 6 11 470 705 

PR 5,405 5,405 3,995 5,640 

SC 2,632 2,632 2,444 2,9 14 

SD 2,820 2,820 2,632 3,008 

TX 2,444 2,444 2,256 2,726 

WI 2,632 2,632 2,444 2,9 14 

TOTAL: 57,058 57,058 55,084 60,489 

Notes: P 1 = Protocol I (breeding and associated data) 

P2 = Protocol 2 (characterization) 

Cry34Abl Cry35Abl Cry3bl 

3,008 3,008 4,324 

658 658 846 

3,008 3,008 4,324 

5,452 5,452 7,097 

6,25 1 6,25 1 9,07 1 

7,050 7,050 10,293 

6,674 6,674 9,682 

658 658 846 

4,37 1 4,371 6,298 

658 658 846 

4,5 12 4,51 2 6,345 

658 658 846 

2, 162 2, 162 3,384 

2,444 2,444 3,666 

658 658 846 

658 658 846 

5,405 5,405 7,990 

3,008 3,008 4,324 

3, 196 3, 196 4,606 

2,726 2,726 3,760 

3,008 3,008 4,324 

66,223 66,223 94,564 

Combined) 

Table 5. Total Pounds of Active Ingredient to be Used by Protein/RNA and State (for Pl + P2 Acrea e Combined) 

State CrylA.105 Cry2Ab2 CrylF DvSuf7 Cry34Abl Cry35Abl Cry3Bbl 

AR 0.0184 0.00553 0.02 16 4.155 x 10'

8 0.283 0.00692 0.0548 

CA 0.00428 0.00 128 0.00522 7.990 x 10·

9 0.06 19 0.00 151 0.0109 

GA 0.0 184 0.00553 0.02 16 4.155 x I 0·

3 0.283 0.00692 0.0548 

HI 0.0270 0.00809 0.0271 7. 19 1 x 10·

8 0.51 2 0.0 125 0.0863 

IL 0.0372 0.01 12 0.0400 9. 189 x 10·

3 0.588 0.0 144 0. 1 IO 

IN 0.0388 0.01 16 0.043 1 1.1 27 x 10·

1 0.663 0.0 162 0. 117 

IA 0.0395 0.0 118 0.0438 1.023 x 10·

7 0.627 0.0 154 0. 11 4 

KS 0.00428 0.00 128 0. 00522 7.990 x 10'

9 0.061 9 0.00 151 0.0 109 

MN 0.0253 0.00760 0.0264 6.232 x 10·

8 0.41 1 0.0 101 0.0781 

MO 0.00428 0.00 128 0.00522 7.990 x 10·9 0.06 19 0.00 151 0.0 109 

9 
EPA EUP No. 62719-EUP-66 

Decision No. 51 1429 

State CrytA.105 Cry2Ab2 

NE 0.0260 0.00780 

NC 0.00428 0.00128 

ND 0.0158 0.00474 

OH 0.0158 0.00474 

OK 0.00428 0.00128 

PA 0.00428 0.00128 

PR 0.0378 0.0 11 4 

SC 0.0184 0.00553 

SD 0.0 197 0.00592 

TX 0.01 7 1 0.005 13 

WI 0.0 184 0.00553 

TOTAL: 0.399 0.120 

CrylF 

0.0292 

0.00522 

0.0 181 

0.0 18 1 

0.00522 

0.00522 

0.04 17 

0.02 16 

0.0223 

0.0202 

0.02 16 

0.448 

Notes: PI = Protocol I (breeding and associated data) 

P2 = Protocol 2 (characterization) 

DvS11.f7 Cry34Abl Cry35Abl Cry3Bbl 

6.152 x 10·3 0.424 0.0104 0.0799 

7.990 x 10·

9 0.0619 0.00 151 0.0109 

3.196 x I o-s 0.203 0.00497 0.0434 

3.675x 10·8 0.230 0.00562 0.0450 

7.990 x 10·9 0.06 19 0.00 151 0.0109 

7.990 x 10·

9 0.06 19 0.00 151 0.0109 

6.792 x 10-s 0.508 0.0124 0.110 

4.155 x 10·8 0.283 0.00692 0.0548 

4.474 x 10-s 0.300 0.00735 0.0579 

3.835 x 10-s 0.256 0.00627 0.0455 

4.155 x 10·8 0.283 0.00692 0.0548 

9.364 x 10·7 6.22 0.152 1.17 

Total amount of protein/RNA in grams (g) is as follows: Cry I A. I 05, 181 g; Cry2Ab2, 54.4 g; Cry IF, 203 g; 

DvSnj7, 4.248 x I 0·

4 g; Cry34Ab I, 2,822 g; Cry35Ab I, 69. l g; and Cry3Bb I, 53 1 g. 

2. You will immediately notify the EPA of any findings from the experimental uses that have a 

bearing on safety (i.e., the EPA requires reporting of any adverse effects from the use of or 

exposure to pesticides). You will also keep records of production, distribution, and performance, 

and make the records available on request to any authorized offi.cer or employee of the EPA. 

3. You must provide a final report to the EPA within 180 days of the expiration of the permit. The 

fina l repo11 must include all of the items set forth in 40 CFR § l 72.8(b)(2) and report on the 

following information: 

• A state-by-state breakdown of actual acreage per protocol; 

• A state-by-state breakdown of actual locations per protocol; 

• Any changes to the stated program, including approved changes to study protocols; and 

• A list of specific counties where studies took place. 

4. Per 40 CFR § l 72.4(b)( l)(v), you must provide to the EPA the name and street add ress of all 

cooperators as soon as available and prior to planting. You must record any changes to this 

information in an updated report and submit that report to the EPA prior to planting. 

5. The label submitted in connection with this application is acceptable and must accompany all 

shipments of seed under this EUP subsequent to receiving this approval. Labels must also be in 

the possession of the user at the time of planting. A stamped, accepted copy of the label is 

enclosed for your records. 

10 
EPA EUP No. 62719-EUP-66 

Decision No. 511429 

6. This EUP is not a limited seed increase registration under section 3 of FIFRA, and plantings 

solely for commercial seed increase are prohibited. 

7. The PIP test plots under this permit must not be within 'l-4 mile of endangered or threatened 

species in the Lepidoptera and Coleoptera insect orders (as listed by the U.S. Fish and Wildlife 

Service) or their Critical Habitats. You must provide the EPA with locations of test plots and an 

analysis of proximity of the test plots to endangered or threatened Lepidoptera and Coleoptera 

species and their designated Critical Habitats prior to planting. 

Should you have questions or concerns regarding the amendment and extension of this permit, please 

contact Jeannine Kausch of the Biopesticides and Pollution Prevention Division by email 

(kausch.jeannine@epa.gov) or by phone (703-347-8920). 

Sincerely, 

irector 

ftice of Pesticide Programs 

Enclosures 

cc: Adrian Enache and Tracy Truesdale, EPA Region 2 (Puerto Rico) 

Fatima El-Abdaoui, EPA Region 3 (Pennsylvania) 

Amber Davis, EPA Region 4 (Georgia, North Carolina, and South Carolina) 

Seth Dibblee, EPA Region 5 (Illinois, Indiana, Minnesota, Ohio, and Wisconsin) 

Eugene Thilsted, EPA Region 6 (Arkansas, Oklahoma, and Texas) 

Jamie Green, EPA Region 7 (Iowa, Kansas, Missouri, and Nebraska) 

Jennifer Schuller, EPA Region 8 (North Dakota and South Dakota) 

Patti TenBrook, EPA Region 9 (California and Hawaii) 

11