# Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

January 2013 Distribution Statement A: Approved for public release; distribution is unlimited.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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# N O T I C E

This document is disseminated under th e sponsorship of th e Department of Homeland Security in the interest of inform ation exchange. The United States Government assumes no liability for its contents or use thereof.

The United States Government does no t endorse products or manufacturers. Trade or manufacturers’ names appea r herein solely because they are considered essential to th e object of this report.

This report does not constitute a standar d, specification, or regulation.

Timothy R. Girton Technical Director United States Coast Guard Research & Development Center 1 Chelsea Street New London, CT 06320Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

iii UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Technical Report Documentation Page

1. Report No. 2. Government Accession Number 3. Recipient’s Catalog No.

4. Title and Subtitle

Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I 5. Report Date January 2013

6. Performing Organization Code

Project No. 410143

7. Author(s)

D. Heilprin, C. Ehrler, Todd Main, Penny Herring 8. Performing Organization Report No. RDC UDI No. 1381

9. Performing Organization Name and Address

U.S. Coast Guard Research and Development Center 1 Chelsea Street New London, CT 06320 SAIC 23 Clara Drive, Suite 206 Mystic, CT 06355-1959 ECORP 3914 Murphy Canyon Rd, Ste A232 San Diego, CA 92123 10. Work Unit No. (TRAIS)

11. Contract or Grant No.

Contract HSCG32-10-D-R00021/ Task Order: HSCG32-10-J-R300004 Task Order: HSCG32-11-J-R300016 Task Order: HSCG32-12-J-R300030 Contract HSCG32-11-P-E55003

12. Sponsoring Organiza tion Name and Address

United States Environmental Protection Agency Great Lakes National Program Office 77 West Jackson Blvd (G-17J) Chicago, IL 60604-3511 13. Type of Report & Period Covered Final

14. Sponsoring Agency Code

United States Environmental Protection Agency Great Lakes National Program Office

15. Supplementary Notes

The R&D Center's technical point of contact is Gail E. Roderick, 860-271-2658, email: Gail.E.Roderick@uscg.mil

.

16. Abstract (MAXIMUM 200 WORDS) This report combines two earlier reports re garding investigations into the potential for barges and towboats to transport Asian carp upstream across the United States Army Corps of Engineers’ electronic dispersa l barrier and release them on the Lake Michigan side of the barrier. It summarizes a series of experiments conducted du ring June 2011 to evaluate the potential for Asian carp larvae to be entrained into and survive in barge balla st tanks on the Illinois River. It also describes investigatio ns in 2010 and 2012 to determine the amount of water normally carried in barge ballast tanks.

Experiments were conducted in the LaGrange Reach of the Illinois River. Results indicat ed few Asian carp larvae were entrained and the majority of entrained fi sh were non-Asian carp, primarily gizzard shad. Survival of Asian carp larvae in test cages in tanks was high, even when water quality conditions were not fa vorable (low dissolved oxygen concentrations). A very small percentage (0.56%) of Asian carp survived for 30 minutes after being pumped through either a 2-inch or 3-inch pump. Although long-term survival following pumping was not determined, this extremely low survival rate tr anslates to a minimal risk.

Visual inspections of ballast tanks and voids on 132 barges (empty and loaded) and 14 towboats were completed in the Chicago Sanitary and Ship Canal (CSSC) in August 2010. An additional tank survey was conducted in July 2012 on barges operating locally near the electronic dispersal barrier s. Overall, only 5 percent of the mo re than 1000 tanks surveyed contained a measurable amount of water. Dissolved oxygen (DO) in tanks ranged between 0.44 - 7.80 mg/L. Although the water quality conditions were not optimal and water depth was very shallow, tank s could support early developmental stages of Asian carp.

Volume I of this report contains the descriptions, results, and conclusions from the experiments and surveys as well as a description of barge design and normal operating procedures. Volume II is comprised of the appendices containing a test plan fo r experiments and field and laboratory data sheets from the original reports.

17. Key Words 18. Distribution Statement

Asian carp, barge ballast tank, water quality, entrainment, larval survival, LaGrange Reach, Illinois River, water transport Distribution Statement A: Approved for public release; distribution is unlimited.

19. Security Class (This Report)

UNCLAS//Public 20. Security Class (This Page) UNCLAS//Public 21. No of Pages 80 22. PriceAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### ACKNOWLEDGEMENTS

Survivability Experiments:

The authors would like to thank the staff of the Illinois Natural History Survey (INHS), Havana Biological Field Station for their assi stance with conducting the field data collec tion on this project. INHS staff who helped included Thad Cook, Levi Solomon, Nerissa Michaels, Stephen Tyszko, Blake Ruebush, Cammy Smith, Greg Sass, T. D. VanMiddlesworth, Eric Hi ne, Greg Fretueg, Chase Holtman, Tad Locher, Kenny Lookingbill, Clint Morgeson, Emma Trone, Heather Po sluszny, Collin Hinz, and Anthony Erickson. We would also like to thank Chuck Burlingame from American River Transportation Company (ARTCO) for supplying the experimental barge and special thanks to his crew for accommodating us during the field work. A special thanks to Phil Moy and Duane Chapman for their re view and input during the planning stages and on the Final Test Plan and on this report. Thanks to Israel Marquez and Dave Krolick of ECORP Consulting for producing the Geographic Information System (GIS) map. Thanks to Susan Helberg, Andrew Harmer, and John Steinbeck of Tenera Envir onmental for their assistan ce with laboratory larval identifications, database setup, data entry, and analysis. SAIC pers onnel who assisted on this project include Steve Ricard, Brad Wilson, and Ron Kesner w ith technical support and Ardeth Mortensen with production support.

Water Transport Surveys: This effort was supported by the Un ited States Environmental Protection Agency, United States Army Corps of Engineers, Illi nois Department of Natural Resources, and the United States Coast Guard. The Coast Guard would like to thank the following companies for providing access to their barges and towboats and for their continued cooperation:

In 2010

• Illinois Marine Towing (IMT)

• American River Transportation Company (ARTCO)

• Hanson Material Services

• Kindra Lake Towing

• American Commercial Lines

In 2012

• Illinois Marine Towing (IMT)

• American River Transportation Company (ARTCO)

• Hanson Material Services

• Midwest Generation, LLCAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### EXECUTIVE SUMMARY

The overall purpose of this project wa s to investigate the potential for Asian carp early life stages to be entrained into and survive in barge ballast tanks which might allow these fish to bypass the United States (U.S.) Army Corp of Engineers (U SACE) electrical dispersal barriers in the Chicago Sanitary and Ship Canal (CSSC). Asian carp are non-native, invasive fish that have been steadily migrating up th e Mississippi River and its tributaries since the mid-1990s. A self -sustaining population of As ian carp exists in the Marseilles pool and Asian carp have been iden tified proximate to the Lockport Lock and Dam. Environmental deoxyribonucleic acid (e-DNA) from Asian carp has been found on the lake side of the USACE electric dispersal barriers. There is concern that Asian carp eggs, larvae, and fry entrained in towboat and barge ballast tanks could be transported upstream past the disp ersal barriers, released above the barriers, then possibly move into Lake Michigan a nd eventually into the rest of the Great Lakes.

In the early 1960s and 1970s, Asian carp (bighead, silver , grass, and black) were brought to the United States from Asia to control submersed aquatic vegeta tion, algae, and mollusks, and also to improve water quality of aquaculture ponds and at wastewater treatment facilitie s. Through pond escapement or by deliberate introductions, these specie s now occur in many of the rivers and back waters throughout the Upper Mississippi River System (UMRS).

USACE has constructed electrical barriers to keep inva sive fish from moving between the Illinois River and Lake Michigan (and the rest of the Great Lakes). The CSSC Dispersal Ba rrier now consists of three barriers comprised of Barrier I (the Demons tration Barrier) and Barrier II c ontaining a set of two independent barriers (IIA and IIB). Each barrier has electrode arra ys spanning the entire width of the canal. The electrodes pulse direct current into the water, which causes fish to turn back rather than pass through the electric current. The barriers currently operate as part of an effort to keep Asian carp from passing through the CSSC and into the Great Lakes, but little informa tion exists on potential transport mechanisms for early life stages of Asian carp, incl uding eggs and larvae.

The project required two separate effo rts: evaluation of the potential for early life stages of Asian carp to survive in barge ballast tanks and a determination of the volume of water being transported across the barriers during normal barge operations.

The survivability study was designed to assess the poten tial for early life stages of Asian carp to be entrained into barge ballast tanks th rough either cracks or holes in the hu ll and then survive there. A hopper barge was modified by instal ling valves on 3-inch holes cut into the exterior of its four ballast tanks to simulate a barge with a ruptured hull. Valves were opened at set times to entrain water and potentially Asian carp larvae. Survival was investigated by placing caged larvae into the flooded tanks for known periods of time. In addition, an experiment to assess pump effects was conducted to determine whether early life stages of Asian carp could survive a single pass through the type of por table pump normally used during barge and towboat ballasting ope rations. Water quality parameters, including temperature, dissolved oxygen (DO), conductivity, potential hydroge n (pH), and ammonia were record ed in the Illinois River, as well as in the barge ballast tanks during each experimental trial.

At selected time intervals, tanks were pumped out and the contents retained in a plankton net were examined to determine whether larvae had been entrained. Only three Asian carp larvae we re found in the pump-outAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

viii UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 material during the entrainment trials. However, b ecause the entrainment and survival experiments ran concurrently, and because some of the mesh used in the experimental cages was damaged during the study, it is possible that these Asian carp larvae escaped from the damaged cages rather than having been entrained. The majority of the fish entrained into the tanks and later pumped out were non-Asian carp (mainly gizzard shad), which were present in relati vely high abundances during some of the river plankton tows prior to the initial tank flooding. Results of the survival experiment indicated high survival (low mortality) rates for containment period s as long as 144 hours in the flooded tanks.

The pump effects portion of this stud y indicated that if entrained, a very small percentage (0.56 percent) of Asian carp larvae could survive a sing le pass through either a 2-inch or 3-inch portable ballasting pump. During normal tank deballasting, no hose is attached to the pump discharge and th e discharged water falls several feet from the barge deck level to the river surface. During this study, however, a discharge hose was attached from the pump to below the waterline to allow the pumped water to be directed into and filtered through a submerged plankton net. It is uncertain what additional mortalit y (if any) might have taken place had no hose been used on the pump’s discharge and th e larvae fell directly from the pump discharge and impacted the river surface. Likewise it is not possible to determine the e ffects of impact with the hose wall.

Water quality parameters measured during the experime nts showed that Asian carp larvae were able to withstand a wide range of water quality parameters. The most variable water quality parameter was DO, which decreased in the tanks from a maximum of 5.51 milligrams per liter (mg/L) to a level as low as 0.86 mg/L in the tanks. However, even at these depre ssed levels, larval survival was not adversely affected.

It should be noted the study began an estimated 11-13 days after an Asian carp spawning event based on the age of the larvae collected in the river at the beginning of the study. Consequently, no eggs or larvae younger than this age were available for testing. It is possible that had the expe riments been conducted in the midst of an Asian carp spawning event (both ti me and location), entrainm ent of their eggs and young larvae may have been higher through the 3-inch test openings since their avoida nce reaction would be less developed.

The water transport study used visual inspections of barge ballast tanks an d measured water depth, temperature and dissolved oxygen (DO) concentration in tanks and voids of barges and towboats to assess the potential for early developmental stages (eggs and larvae) to surviv e and potentially be transported above the barrier. Initial surveys con ducted in the Illinois Rive r in 2010 indicated that most (95 percent) of the 969 barge and towboat ballast tanks inspected were dry. For those barge and towboats that contained measurable amounts (more than 2 - 3 inches) of wate r in an individual tank, wa ter temperatures and DO concentrations were within published tolerances for either Asian carp or other carp species.

A follow-on survey of tanks on barges operating in the immediate vicinity of th e USACE electric dispersal barriers was conducted in July 2012 to determine whether locally operating barges engaged in different ballasting procedures and frequencie s than the barges surveyed in 2010. Initially, the focus was intended to shift from the large barge companies as previously reported to local/small barge prov iders. It was found that there are no small companies that move barges within the dispersal barrier focus area, but a few of the previously visited large companies do designate many of their barges to wo rk in the local barrier area. The inspection team focused on these local groups of barges to collect data on 39 barges rafted along areas of the river and two tugboats. The barges inspected were usually empty and in generally good condition. Discussions with operators revealed that ballasting procedures and freque ncies wer e nearly the same for the local barges as for the longer distance barges survey ed earlier. Similarly, only 6 percent of the local bargeAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

ix UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 tanks inspected contained water deep enough (about 4 inches) to measur e temperature and DO. Temperature and DO readings were at the upper ra nges of those measured in 2010. Results from this survey of barge tanks suggest that water quality conditions in barg e and towboat ballast tanks during one season (summer) would be able to sustain juve nile to adult Asian carp.

Results of the overall study i ndicate that while it may be possible for ea rly life stages to be transported in a barge ballast tank for long periods, there is a very low probability that those life stages will survive passage through the pump when the tanks are deballasted. Additionally, early life st ages are available for entrainment or active pumping for a limited time each year. Therefore, barge ballast tanks present a minimal risk vector for incidental transport and introduction of Asian carp.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### TABLE OF CONTENTS

ACKNOWLEDGEMENTS .............................................................................................................. ........... V

EXECUTIVE SUMMARY .............................................................................................................

.......... VII

LIST OF FIGURES .................................................................................................................................. XIII

LIST OF TABLES .................................................................................................................................... XIV LIST OF ACRONYMS, ABBREVATIONS, AND SYMBOLS ............................................................. XV

1 INTRODUCTION .................................................................................................................. ................ 1

2 SURVIVABILITY OF ASIAN CARP IN BA RGE TANKS IN THE ILLINOIS RIVER .............. 1

3 SURVIVABILITY TEST METHODS ................................................................................................. 3

3.1 Overview ...................................................................................................................... ..................... 3 3.1.1 Barge Location and Setup ........................................................................................................... 4 3.1.2 River Collections and Results ..................................................................................................... 4 3.2 Entrainment Experiment Methods ................................................................................................ .... 8 3.3 Survival Experiment Methods ................................................................................................... ....... 9 3.4 Pump Effects Experiment Methods ............................................................................................... . 12 3.5 Lab Analysis ................................................................................................................................... 12 3.6 Water Quality Analysis ................................................................................................................... 12

4 SURVIVABILITY TEST RESULTS ................................................................................................. 14

4.1 Entrainment Experiment Results ................................................................................................ .... 14 4.2 Survival Experiment Results ................................................................................................... ........ 16 4.3 Pump Effects Experiment Results ............................................................................................... .... 21 4.4 Water Quality Results ..................................................................................................................... 22

5 SURVIVABILITY TEST DIS CUSSION/CONCLUSIONS ............................................................. 25

6 WATER TRANSPORT DURING NORMAL OPERATIONS OF TOWBOATS AND BARGES IN THE ILLINOIS RIVER ................................................................................................ 29

7 SURVEY METHODS ................................................................................................................ .......... 29

8 SURVEY RESULTS ................................................................................................................ ............ 31

8.1 Water Depth .................................................................................................................................... 31 8.1.1 Barges ........................................................................................................................ ............... 31 8.1.2 Towboats ...................................................................................................................... ............. 33 8.2 Water Temperature ......................................................................................................................... 33 8.2.1 Barges ........................................................................................................................ ............... 33 8.2.2 Towboats ...................................................................................................................... ............. 33 8.2.3 Ambient Conditions ............................................................................................................ ...... 34Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### TABLE OF CONTENTS (Continued)

8.3 Dissolved Oxygen (DO) .................................................................................................................. 34 8.3.1 Barges ........................................................................................................................ ............... 34 8.3.2 Towboats ...................................................................................................................... ............. 34 8.3.3 Ambient Conditions ............................................................................................................ ...... 34 8.4 Vessel Traffic .................................................................................................................................. 34

9 SURVEY DISCUSSION/CONCLUSIONS ........................................................................................ 35

10 SURVEY RECOMMENDATIONS .................................................................................................... 37

11 UPDATE FROM 2012 SURVEY OF LOCAL BARGES ................................................................. 37

11.1 Methods Used in 2012 Survey ........................................................................................................ 38 11.2 Results of 2012 Survey ................................................................................................................... 38 11.2.1 Water Depth in Tanks from 2012 Survey ................................................................................. 40 11.2.2 Water Temperatures and Dissolved Oxygen from 2012 Survey .............................................. 41 11.3 Conclusions from 2012 Survey .................................................................................................. ..... 42

12 REFERENCES .................................................................................................................... ................. 43

12.1 References for “Survivability of Asian Carp in Barge Tanks in the Illinois River” ....................... 43 12.2 References for “Water Transport During Normal operations of Towboats and Barges in the Illinois River” .................................................................................................................................. 44

APPENDIX A TEST PLAN ................................................................................................................... A-1

APPENDIX B ASIAN CARP FIELD AND LA BORATORY DATA SHEETS ............................... B-1

APPENDIX C SURVEY PHOTOS, AUGUST 2010 ........................................................................... C-1

APPENDIX D SURVEY PHOTOS, JULY-AUGUST 2012 ............................................................... D-1

APPENDIX E 2010 WATER TRANSPORT FIELD DATA SHEETS ............................................. E-1

APPENDIX F 2012 WATER TRANSPORT FIELD DATA SHEETS .............................................. F-1

APPENDIX G TOWBOAT AND BARGE DESIGN AND OPERATION ........................................ G-1Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### LIST OF FIGURES

Figure 1. Sampling area and barg e location on Illinois River duri ng Trials 1 and 2, June 2011. .................. 5 Figure 2. Photos of the push plankton net frame construction and sampling during river plankton
tows. ......................................................................................................................... ....................... 6 Figure 3. Photos from the entrainment experiment. ............................................................................ ........... 9 Figure 4. Photos from the survival experiment. ............................................................................... ............. 10 Figure 5. Photos from pum p effects experiment. ............................................................................... ........... 13 Figure 6. Example of material (mainly rust) pumped out of ballast tank during Trial 1. ............................. 16 Figure 7. DO concentration in Illin ois River and barge ballast tanks during Trial 1 (top) and Trial 2 (bottom). ..................................................................................................................... ................... 24 Figure 8. Illinois River Ga ge Height at Kingston Mi nes and Copperas Creek.. ........................................... 26 Figure 9. Barge and towboat ba llast tank sampling locations (re d dots) along CSSC (red line), August 2010. ................................................................................................................................. 30 Figure 10. Water depth (inches), water temperature (°F), and DO concentration (mg/L) for deck barge (top) and hopper barge (bottom) ba llast tanks sampled in August 2010. .......................... 32 Figure 11. Water depth (inches), water temperature (°F), and DO c oncentration (mg/L) for towboat ballast tanks sampled in August 2010. ......................................................................................... 33 Figure 12. Annual vessel traffic across the electric fish barrier in 2007 ...................................................... 35 Figure 13. Ballast tank hi nged manhole hatch. ................................................................................ ............. 39 Figure 14. Ballast tank re movable manhole cover. ............................................................................. .......... 39 Figure 15. Ballast tank access tubes. ........................................................................................ .................... 40 Figure 16. Typical tank inspection view. ...................................................................................................... 40 Figure 17. Water Temperature and Dissolved Oxygen Comparisons. ......................................................... 41 Figure C-1. Loaded (upper) a nd unloaded (lower) barges. ...................................................................... ... C-1 Figure C-2. Interior of em pty hopper barge (upper). Load ing hopper barge (lower). ................................ C-2 Figure C-3. Raised and flush hatc h covers on rafted barges (Upper). Raised access hatch (lower). ......... C-3 Figure D-1. Hopper barge dry-docked for repair, not e hull depth to freeboard (upper). Hopper barge fully loaded with construction grade sand (lower). ................................................................ D-1 Figure D-2. Loaded barges rafted at construction ma terials site (upper). C overs being installed to protect cargo on hopper barge; no te added height (lower). .................................................... D-2 Figure D-3. Barge being loaded with loose materi al near dispersal barr ier (upper). Empty hopper barge at Midwest Generation on CSSC (lower). .................................................................... D-3 Figure D-4. Recording data aboard towboat in CSSC (upper). Warn ing sign on CSSC near dispersal barrier (lower). ..............................................................................................................

.......... D-4Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### LIST OF TABLES

Table 1. Date, location, and larval fish in river collections. ............................................................... ............ 7 Table 2. Summary of filling/emptying dates and times plus water depths of varying tank fills during Trials 1 and 2. ............................................................................................................... .................... 8 Table 3. Proposed and actual exposure times (hours) for survival experiment Trial 1 test cages. ............... 11 Table 4. Proposed and actual exposure times (hours) for survival experiment Trial 2 test cages. ............... 11 Table 5. Summary of stage number, stage name, le ngth, and days post-fertiliz ation for silver carp larvae from translation of Yi et al. (1988) by Chapman (2006).. ................................................... 14 Table 6. Number of fish larvae collected after pu mp-out of ballast tanks following different exposure periods during Trials 1 and 2. ......................................................................................................... 15 Table 7. Ballast tank cages: number of aliv e and dead Asian carp, non-Asian carp, and damaged larvae after varying exposure period s in cages during Trials 1 and 2. ........................................... 18 Table 8. Control Cages: Number of alive and dead Asian carp, non-Asian carp, and damaged larvae after varying exposure periods during Trials 1 and 2. .................................................................... 19 Table 9. Ballast tank cages: summ ary of number of alive and dead Asian carp larvae of each stage for three test cages deployed for different exposure intervals during Trials 1 and 2. ..................... 20 Table 10. Control cages: summary of number of alive and dead Asian carp larvae of each stage for three cages deployed for different exposur e intervals during Trials 1 and 2. ............................. 20 Table 11. List of non-Asian carp larvae used in th e survival experiments or collected during river sampling. ..................................................................................................................... .................. 21 Table 12. Summary of observations of larvae follo wing passage through a 2-inch or 3-inch pump and hose.. ........................................................................................................................ ...................... 22 Table 13. Summary of water qua lity measurement reco rded during Trials 1 and 2, June 2011. ................. 23 Table 14. Vessel company and location of barge and towboat sampling, August 2010. ............................. 31 Table 15. Vessel Company and Location of Barge and Towboat Sampled, July-August 2012. .................. 38 Table 16. Barge Tank Water Content, July-August 2012. ........................................................................ .... 41Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### LIST OF ACRONYMS, ABBR EVATIONS, AND SYMBOLS

μS/cm microSiemens per centimeter ARTCO American River Transportation Company oC degrees Centigrade CAWS Chicago Area Waterway System CSSC Chicago Sanitary and Ship Canal DNA deoxyribonucleic acid DO dissolved oxygen e-DNA Environmental deoxyribonucleic acid EPA Environmental Protection Agency ft feet GIS Geographic Information System

GLRI Great Lakes Restoration Initiative IDNR Illinois Department of Natural Resources IMT Illinois Marine Towing in inch INHS Illinois Natural History Survey KLT Kindra Lake Towing LTRMP Long Term Resources Monitoring Program

m 3 cubic meter mg/L milligrams per liter mm millimeter
nc not collected NIS Non-indigenous species pH potential hydrogen R river RDC Research & Development Center TAN total ammonia nitrogen ULT Upper lethal temperature U.S. United States UMRS Upper Mississippi River System USACE United States Army Corps of Engineers USCG United States Coast Guard USGS U.S. Geological Survey YSI Yellow Springs InstrumentAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### 1 INTRODUCTION

This report is a combination of two earlier reports to en sure that all information from the effort to assess the potential risk for early life stages of Asian carp to be transported across the U. S. Army Corps of Engineers electronic dispersal barrier is availa ble in one consolidated report. Thes e efforts included experiments to determine the likelihood of early life st ages being entrained into and surviv e in barge ballast tanks as well as surveys of barge ballast tanks to determine the amount of non-potable water bei ng transported across the barriers in tanks. Due to the si ze of data appendices, this re port consists of two volumes.

Volume I contains the full text from “Survivability of Asian Carp in Barge Tanks in the Illinois River” as the primary report and that from “Wat er Transport during Normal Operations of Towboats and Barges in the Illinois River” as supporting information. Sections 2 – 5 are the complete text from “Survivability of Asian Carp in Barge Tanks in the Illinois River”. Sections 7 – 12 are the complete text from “Water Transport during Normal Operations of Towboats and Barges in the Illinois River”. Appendix F, “Towboat and Barge Design and Operation”, provides a description of barge design and operating procedures used on commercial barges.

Volume II is comprised of appendices to the two earlier reports that are now cons olidated in Volume I. Volume II includes the test plan for the entrainment e xperiments described in “Survivability of Asian Carp in Barge Tanks in the Illinois Rive r” as Appendix A. The field and laboratory data sheets from the survivability experiments are Appendices B1-B9. Fi eld data sheets from the tank surveys are Appendices C and D.



### 2 SURVIVABILITY OF ASIAN CARP IN BARGE TANKS IN THE ILLINOIS RIVER

The purpose of this study was to inves tigate the potential for early life stag es (eggs, larvae, or juveniles) of Asian carp to be entrained into and survive in barge ballast tanks. Specificall y, this study was designed to address the question of whether barges and towboats can potentially provide a means for these stages to bypass the United States (U.S.) Army Corp of Engin eers (USACE) electrical di spersal barrier in the Chicago Sanitary and Ship Canal (CSSC). A previous survey of 969 ballast tanks on barges and towboats showed that only five percent of the tanks c ontained more than a few inches of water.

Asian carp (bighead, silver, grass, and black) were brought to the United States in the early 1960s and 1970s and now inhabit the waters of the Upper Mississippi River System (UMR S). These species were used to control submersed aquatic vegetation, algae, and mo llusks in ponds thereby im proving water quality of aquaculture ponds and in wastewat er treatment faciliti es (Chapman & Hoff, 2011) and “to develop nonchemical and environmental friendly control mechan isms” (Kelly et al., 2011). Asian carp invaded nearby rivers through pond escapement or by deliberate release. Asian carp reproduce rapidly when the conditions are favorable. All four species of Asian carp are now well established in the Mississippi River basin (Chapman & Hoff, 2011). Biol ogists, policy makers, and citizens are deeply concerned about the prospects of Asian carp entering La ke Michigan through the Chicago Ar ea Waterway System (CAWS). Sparks et al. (2011) stated that if bighead and silver carp enter Lake Michigan, they will directly compete for food resources with a number of well-established sport fishes.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

2 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 The United States Army Corps of En gineers constructed a permanent elect rical barrier (the CSSC Aquatic Nuisance Species Dispersal Barrier) to protect Lake Michigan and the other Great Lakes from invasive species movements between the Mississippi River and lake s. This electric barrier was originally conceived as a method to prevent the movement of non-native fishes through the CAWS. The round goby was the first target species but it moved out of La ke Michigan and through the proposed barrier location into the Illinois River before construction of the orig inal barrier was complete. The CS SC Dispersal Barrier consists of three electric barriers (formally Ba rrier I and II) with Barrier II havi ng two independently operating barriers (IIA and IIB), each consisting of 42 electrodes spanning th e width of the canal. The electrodes pulse direct current into the water at a strength and frequency that causes fish to turn back rather than pass through the electric current. The barriers are operating in an effort to keep Asian carp from moving upstream and possibly entering Lake Michigan.

Bighead and silver carp have recently been found downstream of the CSSC and environmental deoxyribonucleic acid (e-DNA) from th ese species has been found upstream of the barrier (Moy et al., 2011; Jerde et al., 2011). Although the actual source of th e positive e-DNA hits has not been found, experiments have shown that e-DNA can persist in water up to 48 hours (Jerde et al., 2010). Towboat operators have reported that Asian carp will occasionally land on the towboat or barge and either die there or leave blood and mucous on the deck until the boat crew hoses down the deck. Thus, it is possible that Asian carp e-DNA could be carried across the ba rriers by vessel traffic.

There is also concern that Asian carp eggs, larvae, a nd juveniles may be taken up in ballast or leakage water of towboats and barges and potentially be transporte d through the dispersal barr ier. If the fish are discharged upstream of the dispersa l barrier, there is concern that As ian carp could advance into Lake Michigan. While the barrier operates to keep Asian carp from continuing to move upriver, little information exists on potential transport m echanisms for early life stages of Asian carp (Moy et al., 2011).

Irons et al. (2011) reporting on samp ling in the La Grange Reach of the Illinois River from 1990 through 2006 stated that the catch of adult silver and bighead carp began in 2000. The peak in abundance of bighead carp was in 2000 followed by a decline to very low leve ls in 2005 and 2006. Silver carp abundance peaked in 2004 and then decreased between 2004 and 2006 in th e La Grange Reach, howev er Irons et al. (2011) stated that the sampling equipment was ineffective in the collection of the largest individuals, with individuals being observed swimming away from the capture boat. They also observed good recruitment of both bighead and silver carp (based on catch of fish less than about 200 millimeter [mm]) during 2000 and 2003. However, recruitment of both species was poor in 2001, 2002, and 2005. Sass et al. (2010) reported that the La Grange Reach of the Illinois River had a conservative esti mate of 2,544 silver carp with a biomass of 5.5 metric tons per ri ver kilometer during 2007-2008. They estimated that silver carp made up about 51 percent of the total fish collected by the Long Term Resour ce Monitoring Program (LTRMP) in the La Grange Reach. No estimate of bighead carp abundance was presen ted in this paper.

Papoulias et al. (2006) found th at bighead and silver carp can have a long annual spawning season each year in the Missouri River and appear to be able to spawn more than one tim e during each year. They found that from 2003 through 2005, silver carp spawned as early as late March when th e water temperature had reached about 14 oC while bighead carp did not begi n spawning until about early May when the temperature was about 19-20 oC. Irons et al. (20 11) stated that the critical spawni ng tem perature for bigheaded carp was 18 oC. Coulter & Goforth (2011) found Asian carp eggs in the Wabash River, Indiana, from May through July during 2011. Several studies, including Jennings (1988), DeGrandchamp et al. (2007), Kolar et al.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

3 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 (2007), Lohmeyer & Garvey (2009), and Irons et al. (2011) also stated that Asian carp spawning is positively correlated to increasing river flow. DeGrandcha mp et al. (2007) also stated that high river flow does not appear to be critical fo r reproduction to take pl ace but may aid in egg and larval survival.

Yi et al. (1988, as translated in Chapman, 2006) described the characteris tics of the 48 early developmental stages of the eggs and larvae of the four species of Asian carp in the Yangtze Ri ver. These stages begin with egg fertilization and end with the larvae transition into a juvenile. Egg deve lopment to hatching takes place quickly. Yi et al. (1988) reported that bighead car p in the field hatch about 39 hours after fertilization while Chapman & George (2011) found hatching in the laboratory after 31 to 45 hours depending on incubation temperature. Silver carp hatch between 26 to 45 hours afte r fertilization in the laboratory (Chapman & George, 2011) while Yi et al (1988) reported hatching in the Yangtze River after about 38 hours. Development to the juvenile stage in silv er and bighead carp was estimated to take about 60-70 days after fertilizati on (Yi et al. 1988).

Chapman & George (2011) reported th at newly hatched bighead and silv er carp larvae imme diately begin a vertical swimming motion when they were not resting; this swimmi ng motion changes to a horizontal direction near the water su rface at larval Stage 36 (w hen the eyes become pigmented) which is about 100 hours after fertilization. By Stage 38 at about 146 hours after fertilizatio n (referred to as the one-chambergas-bladder stage), larvae swim horiz ontally at varying depths between re sting periods. These authors also reported that larvae began to move out of the river channel into the slower water near shore about 100+ hours post hatching (about Stage 37 when the gas bladder forms).

The current study was designed to evalua te the potential for entrainment of early life stages of Asian carp into barge tanks via leaking hulls and to assess their survival in barge ballast water. Another aspect of the study was to assess survival of thes e life stages afte r being pumped through a portable pump. This study was not designed to directly address th e question of survival of early life stages being transported across the electric barriers while in a ballast tank.



### 3 SURVIVABILITY TEST METHODS

3.1 Overview Two experimental trials, each las ting approximately 7 days, were conducted from June 11-24, 2011, within the La Grange Reach of the Illinois River, just down river (south) of Peoria, IL. River plankton sampling began June 9 in an attempt to locate high concentr ations of Asian carp eggs and young larvae. With few exceptions, all field and laboratory methods followed an approved Study Plan (Volume II, Appendix A). Details concerning minor changes to the approved Study Plan are presen ted below. Modifications ranged from changes in the experimental loca tions to changes in cage holding times.

The decision to begin in early Ju ne was based on the suggestion of Dr. Greg Sass (pers. comm., June 1, 2011, Director, Illinois Natural History Survey (INHS), Havana Biological Fi eld Station). He felt that the river water temperature was right fo r spawning and the river was rising du e to thunderstorms in the region. He also mentioned that there ha d been reports of Asian carp swimming at the surface with possible spawning having already taken place. By June 9 when sampling began, the river was 6-8 feet above its average height for that time of year and was consider ed to be in flood conditions, which had a direct impact on both sampling and testing locations (see Section 4).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

4 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 3.1.1 Barge Location and Setup All experiments took place on a hopper ba rge secured to the outboard side of a deck barge. The barges were provided by Am erican River Transportation Company (ARTCO) and were moored at the ARTCO Fleeting Services dock in Pekin, IL ( Figure 1). ARTCO modified the four ballast tanks of the hopper barge by drilling one 3-inch d iameter hole be low the waterline in the side of each tank and fitting each with a gate valve to allow tank flooding. The modification was intende d to simulate a leak for potential entrainment of larvae. Each of the four ballast tanks on the modified barge was filled by opening the valve and then emptied using a Honda WB20X (2-inch hose) or WB30 X (3-inch hose) pump. These types of pumps are used by most barge operators during normal operations to fill or empty their ballast tanks. The ballast water was pumped out of each tank and into a plankton net suspended in the river water in a spud well on the adjacent deck barge. The pump’s discharge hose emp tied below the waterline inside the plankton net..

3.1.2 River Collections and Results Plankton tows were used to identify areas where As ian carp eggs and larvae could be found in the La Grange Reach of the Illinois River. Tows were conducted on 9-10 June pr ior to Trial 1, on 18 June prior to Trial 2, and on a few days during both trials. The m ajo rity of the tows were conducted using a plankton net attached to a wood frame ( Figure 2) lowered off the bow of a boat supplied by the IN HS, Havana Biological Field Station. In general, tows were run diagonally across the channe l from one shore to the other.

Results of these tows and visual obs ervation indicated that no Asian carp eggs and very low concentrations of their larvae were present in the channel location; how ever, larval Asian carp were present in significantly higher abundances in shallow water ha bitats along the shoreline, genera lly between the flooded tree trunks. Consequently, larvae were collected using small dip nets from shallow water lo cations along the shoreline and were used during both trials for the survival and pump effects studies. All Asian carp larvae collected in the larger nets in the main river channel were ge nerally of the same size as those collected in higher numbers along the shoreline near the flooded tree line.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

5 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure 1. Sampling area and barg e location on Illinois River du ring Trials 1 and 2, June 2011.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

6 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Plankton net frame construction Plankton net on frame

Plankton net being lower into the water Identifying organisms collected in plankton net using headband magnifier Figure 2. Photos of the push plankton net frame c onstruction and sampling during river plankton tows.

A second result of finding few to no larvae in the main channel was that it would not be possible to follow one cohort of larvae as they drifted downriver as was in itially planned. It was ther efore decided to moor the experimental barge and an additional deck barge at th e ARTCO facility in Pekin with the valve openings at the edge of the channel thereby allowing rive r water to flow freely past the openings.

Table 1 presents a summary of the plankton net tow da tes, locations, and the m ain species of fish larvae found on each collection day. For the first two collections (9-10 June), a 500 micron mesh net was towed from the side of the boat in the upper 3-6 ft (feet) of the water column for about 5 minutes. Generally, the rest of the tows were about 5-10 minutes long using a 500-micron mesh net suspended off the front of the boat while it was motored upstream either into or acro ss the river flow. This net setup filtered water from approximately the upper 30 inches (in) of the water colum n. The majority of the tows were across the river from bank to bank. The volume of water was not determin ed during the first series of tows, as the emphasis was to try to locate high c oncentrations of Asian carp eggs or larv ae in the main channel and along the shoreline. If a high density had been found, a flowmete r would have been attached to the net to determineAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

7 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 volume filtered to find the actual egg or larval concentration. During the 22-23 June tows, a flowmeter was added to the net to generate an estimate of the water volume filtered. The volume per tow ranged from about 100 to 235 cubic meters (m 3 ) (~26,000 to 62,000 gallons). As can be seen in the table, no Asian carp eggs and only low abundances of their larvae were found.

Table 1. Date, location, and larv al fish in river collections.

Note: The total number of all larvae collect ed during each tow was not determined*.

Date Location Number of Tows Larval Fish 6/9/11 ARTCO’s launch ramp to Pekin Landing. Tows were conducted within the upper 6 ft of the water column either across the channel or along the shore just outside of the tree line. 6 Mainly gizzard shad. Four Asian carp total in all six tows.

6/10/11 ARTCO’s launch ramp to Kickapoo Creek and in Copperas Creek area. Main tows were within upper 3-6 ft of the water column either across the channel or along the shore just outside of the tree line. 5 Asian carp found along shoreline in rock rubble and in water around trees. River and creek tows collected mainly gizzard shad, with about 50 Asian carp total for all tows. 6/15/11 Up and down river from Havana (estimate covered 15 miles of river while sampling). Main tows within about the upper 30 inches of the water column either across the channel or along the shore just outside of the tree line. 9 5-50 Asian carp per tow with most of the larvae being gizzard shad. Highest number of Asian carp was near Liverpool.

6/16/11 Sampled from Pekin to Upper Peoria Lake. Main tows within about the upper 30 inches of the water column either across the channel or along the shore just outside of the tree line. 6 2-20 Asian carp per tow with most of the larvae being gizzard shad.

6/22/11 Sampled from Pekin to Peoria Lock and Dam (estimate covered 4-5 river miles while sampling). Main tows within about the upper 30 inches of the water column either across the channel or along the shore just outside of the tree line. Volume per tow varied from about 110 to 235 m 3 (~29,000- 62,000 gallons). 5 0-5 Asian carp per tow with most of the larvae being gizzard shad.

6/23/11 Up and down river from Havana (estimate covered 6-8 miles of river while sampling). One sample conducted at Toehead Island which historically has been area with high spawning activity. Main tows within about the upper 30 inches of the water column either across the channel or along the shore just outside of the tree line. Volume per tow varied from about 100 to 130 m 3 (~26,000-34,000 gallons). 5 No Asian carp. Larvae mainly gizzard shad.

*Total number of all larval fish for tows presented in this table ranged from 0 to an estimated 500 per tow. The highest numbers were due mainly to gizzard shad coll ected between upper and lower Peoria Lakes.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

8 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 

### 3.2 Entrainment Experiment Methods To evaluate potential entrainment of larval Asian carp into barge ballas t tanks, four experimental tanks on the hopper barge were flooded, emptied, and contents anal yzed during Trials 1 and 2 according to the timing presented in Table 2 (see Volume II, Appendix A, Table 2 for planned timing). As ian carp en trainment experiments ran concurrently with th e survival experiments (see Section 3.3 below). A plankton net (500 m icron mesh) was suspended in river water inside a spud well on the adjacent deck barge while each tank was emptied into the net using a portable pump and discharge hose positioned below the water surface inside the net ( Figure 3). A 2-inch pump was used during th e first pump-out only; a 3-inch pump was used to empty the tanks during all other pump-out events. The pump operated at full throttle during pump out process until the tank water level reached approximately 6 inches re maining. The pump rate was then reduced to about half-throttle to avoid losing suction. (Such rate reduction is common practice during normal barge operations.) The plankton net cod ends were emptied and analyzed twice during each tank pump-out. All entrained larvae, live or dead, were removed and placed in labeled vials for subsequent laboratory identification and staging. The volume of water pumped from each tank was estimated using the dimensions of the tanks supplied by ARTCO and th e depth of water prior to and after pumping.

Table 2. Summary of filling/emptying dates and times plus water depths of varying tank fills during Trials 1 and 2.

Start Date Location Tank Fill Date Filled Time Filled Date Emptied Time Emptied Filled Water Depth

(in.)

Trial 1 6/11/11 Pekin Dock T1 F1 6/11/11 9:25 06/11/11 17:30 51 6/11/11 Pekin Dock T1 F2 6/11/11 21:23 06/17/11 9:30 48 6/11/11 Pekin Dock T2 F1 6/11/11 9:25 06/12/11 9:30 54 6/11/11 Pekin Dock T2 F2 6/12/11 12:00 06/17/11 9:15 50.5 6/11/11 Pekin Dock T3 F1 6/11/11 9:37 06/13/11 9:30 44 6/11/11 Pekin Dock T3 F2 6/13/11 12:12 06/17/11 10:45 47 6/11/11 Pekin Dock T4 F1 6/11/11 9:30 06/14/11 9:35 41 6/11/11 Pekin Dock T4 F2 6/14/11 11:50 06/17/11 11:45 48 Trial 2 6/18/11 Pekin Dock T1 F1 6/18/11 11:20 06/24/11 11:45 56 6/18/11 Pekin Dock T1 F2 No Fill #2 6/18/11 Pekin Dock T2 F1 6/18/11 10:35 06/19/11 10:30 59 6/18/11 Pekin Dock T2 F2 6/19/11 13:32 06/24/11 9:45 45 6/18/11 Pekin Dock T3 F1 6/18/11 11:00 06/20/11 10:45 48 6/18/11 Pekin Dock T3 F2 6/20/11 13:10 06/24/11 10:00 48 6/18/11 Pekin Dock T4 F1 6/18/11 11:10 06/21/11 10:55 49 6/18/11 Pekin Dock T4 F2 6/21/11 13:00 06/24/11 11:30 48

Due to the low concentration of larvae found during ri ver tows prior to Trial 1, additional plankton tows were not conducted during tank flooding. However, a plankton net was deployed alongside the barge while the ballast tanks were being flooded for the entrai nment experiment during Trial 2. The 500 mm mesh plankton net ( Figure 3, lower right) was deployed to a depth sim ilar to the tank valve depth to determine ifAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

9 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Asian carp or other species of larv al fish were present in the wate r column and could potentially be entrained.

Pumps for emptying tanks Pump and hose setup

Emptying tank into plankton net in spud well Plankton net Figure 3. Photos from the entrainment experiment.



### 3.3 Survival Experiment Methods As mentioned above, plankton tows were used to identi fy areas in the La Grange Reach where Asian carp eggs and larvae could be obtained. Results of these tows indicated that eggs were absent and larvae were not present in high enough numbers to conduct the surv ival study in the channel location itself. Larval Asian carp but not eggs were present in shallow water ha bitats along the shoreline, therefore larvae used in the survival experiments were collect ed with dip nets from shallow wate r locations along the shore. For Trials 1 and 2 approximately 30 la rvae were placed in each of th ree cages in each ballast tank ( Figure 4) for a range of exposure times as shown in Table 3 and Table 4, respectively. In a ddition, control cages were deployed in the river during both trials for various exposure tim es, as indicated in Table 3 and Table 4.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

10 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Example cage used in survival experiment Example of larvae collected for survival experiment

Preparing cages for survival experiment Cages suspended in ballast tank

Control cages in river (strong current area) Control cages in river (weak current area)
Figure 4. Photos from the survival experiment.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

11 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 3. Proposed and actual exposure times (hours) for survival experiment Trial 1 test cages.

Cage Set Proposed Exposure Times (hrs) Actual Exposure Times (hrs)

1 8 8 2 24 24 3 48 48 4 72 72 5 80 72* 6 92 94* 7 104 117* 8 108 132*

Control 1 8 8 Control 2 24 24 Control 3 48 65* Control 4 72 72 Control 5 96 118* *Times changed from Final Test Plan ( Error! Reference source not found. )

Table 4. Proposed and actual exposure times (hours) for survival experiment Trial 2 test cages.

Cage Set Proposed Exposure Times (hrs) Actual Exposure Times (hrs)

1 8 144* 2 24 144* 3 48 144* 4 72 144*

Control 1 24 143* Control 2 48 143* Control 60** 72 120* Control 90** 72 120* *Times extended over Final Test Plan (Volume II, Appendix A) **The 60 and 90 indicate the total number of larvae that were initially placed in these control cages.

Cages were 5-gallon plastic bu ckets with lids. Openings cut in the bucket sides and lids were covered with 500-micron mesh netting to allow water circulation ( Figure 4). Keeping the mesh attached over the cage opening was a problem once the trials began. Initiall y, aquarium-grade silicone was used but it was determined that this did not bond securely enough to th e bucket walls. Hot glue used along the edge of the mesh generally secured the mesh adequately for the testing. A few cages requi red repair to the mesh covering more than once.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

12 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 

### 3.4 Pump Effects Experiment Methods The pump effects experiments assessed whether larvae were able to survive the pum ping process that occurs during ballasting operations. This study occurred in c onjunction with the entrainment and survival studies. Larvae for this experiment were collected at shore lo cations using similar methods to those described above for the survival experiment (Section 3.3). The pump intake hose was pla ced in the river and a large num ber of larvae were released from a 5- gallon bucket into the water near the intake hose while the pump was operating. Larvae were sucked through the hose and pump and were discharged into a plankton net suspended in a spud well, similar to methods used for the entrainment study ( Figure 5). Two-inch and 3-inch water pum ps (Honda WB20X a nd WB30X pumps, respectively) were tested during both trials. No count was made in the field of the nu mber of larvae initially re leased near the hose inta ke or collected in the plankton net cod end during each test run. The number of larvae coll ected during one pump test was estimated by counting the number of larvae in a photo taken following collection. This estimate of the number of larvae passing through the pump was used for all the other pump effects tests.

Samples collected in the plankton net were placed in wa ter in small plastic contai ners and the number of live larvae recorded immediately after colle ction. The sealed containers were then placed in a bucket of water to keep the samples’ water temperature stable dur ing a 30-minute holding period. After 30 minutes the number of live larvae was recorded to assess potential effects of pumping on th eir viability after passing through the pump assembly.



### 3.5 Lab Analysis Labeled vials containing the live a nd dead larvae from the entrainment and survival experiments and the river-collected larvae were returned to the lab for identification, st aging, and enumeration. All larvae that survived passing through the pump in the pump effects experiments were also placed in labeled vials and returned to the laboratory for processing. The stage number of each Asian carp larvae ( Table 5) was determ ined based on Yi et al. (1988). Data from the entrainment, surv ival, and pump effects samples were recorded on laboratory datasheets. The stage and status (live or dead) of each Asian carp larva was recorded for the entrainment and survival trials. The number a nd status was also recorded for each non-Asian carp larva collected during these experiment s. All recorded data were entere d into a Microsoft® Office Access® database and verified for complete ness and accuracy prior to the development of summary tables.



### 3.6 Water Quality Analysis Water quality parameters from the flooded tanks and the Illinois River were record ed during the entrainment and survival experiments based on the filling and emptyi ng schedule (Table 2). Water quality data included tank water depth, water temperature, dissolved oxyge n (DO), potential hydrogen (pH), and total ammonia nitrogen (TAN). All water quality samples were collect ed at the water surface in the ballast tanks and the river. A Yellow Springs Instrument (YSI) water quality probe was used to collect temperature and DO. During Trial 1, total ammonia was measured using an AP I® Freshwater Ammonia Test Kit. No pH testing was conducted during Trial 1. During Trial 2, ammoni a and pH were measured using API Freshwater Ammonia and pH Test Kits, respectively.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

13 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Impeller from 3-inch portable water pump Hose location for pump effects experiment

Example of larvae used for pump effects experiment Releasing larvae into river near pump intake

Larvae and juvenile fish (approximately 3 inches) condition after going through pump Figure 5. Photos from pump effects experiment.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

14 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 5. Summary of stage number, stage name, length, and days post-fertilization for silver carp larvae from translation of Yi et al. (1988) by Chap man (2006). Stages 1-30 were egg development stages.

Stage # Stage Name Length (mm) Hours or DaysPostFertilization 31 hatching 6.1 38 hours 32 rudimentary-pectoral-fin 6.3 48 hours 33 gill-arch 6.8 53 hours 34 xanthic-eye 7.2 63 hours 35 gill-filament 7.6 74 hours 36 melanoid-eye 8.0 92 hours 37 gas-bladder-emergence 8.2 106 hours 38 one-chamber-gas-bladder 8.5 146 hours 39 yolk-absorption 8.7 168 hours 40 dorsal-fin-differentiation 9.0 212 hours 41 notochord-tip-lifting 9.4 240 hours 42 two-chamber-gas-bladder 10.3 279 hours 43 pelvic-fin-bud 11.2 312 hours 44 dorsal-fin-formation 14.1 19 days 45 anal-fin-formation 15.7 22 days 46 pelvic-fin-formation 17.0 25 days 47 squamation 20.0 34-55 days 48 juvenile 34.0 60-90 days



### 4 SURVIVABILITY TEST RESULTS

4.1 Entrainment Experiment Results The volume of water pumped out of each ballast tank wa s determined using the dimensions of the tanks provided by ARTCO. The depth of wate r in the ballast tanks varied from 41 to 59 inches with a mean depth of 49 inches. In spite of splitting the ballast tank pump out into two collection periods, the net’s cod end captured a significant amount of rust. To avoid damage to captured larvae from additional rust particles, pumping was halted when an estimated average of 4.5 inches of water remained in each tank. The average volume pumped from the tanks during the two trials wa s calculated to be 9,092 ga llons with a minimum of 8,183 gallons and a maximu m of 10,203 gallons ( Table 6). The water depths used to estimate the volume of water pum ped from each tank are provided on field data sheets in Appendix B1 (See Volume II for appendices.) All other field and laboratory datasheet s are presented in Volume II, Appendices B2 through B9.
The number and type of larvae found in the material pumped out of the ballast tanks during the entrainment studies are also presented in Table 6. In all instances the pump-out m aterial included a large amount of rust particles ( Figure 6). All larvae, regardless of species, were found dead when they were removed from the
pum p-out material from the tanks. It could not be determined if the larv ae died in the tanks, were killed by passing though the pump and hose, or were killed by bei ng hit or smothered by rust while in the cod end. Only three Asian carp larvae, one e ach of stage 41, 44, or 45, were found dur ing these collecti ons. It is not certain if these Asian carp larvae were entrained in to the ballast tanks during flooding or if they escapedAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

15 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 from a damaged cage from the survival study. The majo rity of the fish collected during the entrainment study were non-Asian carp, mainly gizzard shad. Gi zzard shad were found in relatively high abundance during some of the river tows in the days leading up to the initial flooding for Trial 1. A few damaged larvae that could not be visually identified were also collected. The la boratory datasheets for the entrainment experiment are presented in Volume II, Appendices B3 and B6.

Table 6. Number of fish larvae collected after pu mp-out of ballast tanks following different exposure periods during Trials 1 and 2. Note that all larv ae were dead. Blank cells mean no larvae found.

Tank # Fill # Hours Exposed Asian Carp Larvae by Stage NonAsian Carp Larvae Damaged Larvae Volume Pumped from Tank

(gallon) 41 44 45

Trial 1 1 1 8 2 9,305 2 1 24 1 9,642 3 1 48 13 8,519 4 1 72 1 3 14 8,183 4 2 72 1 8,968 3 2 94 1 8,856 2 2 117 9,249 1 2 132 1 8,968 Trial 1 Total 1 0 1 21 14 71,690 Trial 2
1 1 144 1 9,866 2 1 24 1 1 10,203 3 1 48 5 8,968 4 1 72 1 2 1 9,080 4 2 70.5 2 8,968 3 2 96 8,968 2 2 115 8,632 1 2 Tank 1 only filled one time during this trial Trial 2 Total 0 1 0 11 2 64,685Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

16 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure 6. Example of material (mainly rust ) pumped out of ballast tank during Trial 1.

During Trial 2, icthyoplankton samples were collected in the river alongs ide the tug and barge while the ballast tanks were being filled. Two to three samples we re collected each day during each of the four filling events (at 0, 24, 48, 72 hours). The volume of water fi ltered through the plankton net varied from 6,399 to 16,970 gallons with a per sample average of 8,366 gallons . (Scanned field datasheet example is shown in Volume II, Appendix B). The density of larval fi sh collected during these alongside collections was calculated at 33 to 48 individuals per 9,000 gallons (the approximate average volume of water pumped from each ballast tank). No Asian carp larvae were found in any of these river samples. The larval fish concentration in the river was higher than that found in the water pumped from the ballast tanks during this trial. The actual reason for the difference is unknown but could be due to a number of causes including, but not limited to, larval patchiness, avoidance activity by th e advanced stage of larvae, and the difference in the capture efficiency of the open plankton net versus the su ction created by the 3-inch hole in the ballast tanks.



### 4.2 Survival Experiment Results

Survival tests were conducted from 11-24 June 2011 us ing fish larvae collected from near the shoreline, generally from around submerged trees. The larvae were of the same general size as the few found in the channel.

The larvae used for the testing were a mixture of both Asian carp and non-Asia n carp larvae. Initial attempts to separate the larval sp ecies with the aid of a dissecting microscope subj ected the larvae to too much handling and damaged them. Therefore, both Asia n carp and non-Asian carp were placed into the test cages to minimize handling of the collected larvae. A higher proportion of Asian carp larvae made up the larval mixture collected during Trial 2 than Trial 1 due to the decrease in the abundance of non-Asian carp larvae in shoreline collection areas.

The target initial count was 30 larvae per test cage. As indicated in Section 3.3, some of the mesh on several cages becam e loose during the testing due to failu re of the adhesive. This potentially allowed some larvae to escape, causing a reduction in the total number of larvae remain ing in a cage at the conclusion of the test interval for that cage.

Control cages were placed in two areas in the river near the barge. Duri ng the first two days in Trial 1, the control cages were lowered into th e river alongside the tug, but the fast river flow and large amount of floating debris in this location caused extensive damage to the mesh of th e cages, allowing larvae to escape.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

17 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Therefore, the location of the contro l cages was changed to a more “protected” area for the remainder of the survival experiment. All control cages for most of Trial 1 and all of Trial 2 were located in the same protected area where the river current was substantially reduced and there was less large floating debris such as logs and branches. (See Figure 4.)

The control cages in both locations experienced higher sediment loads th an cages in the tanks, thus the control larvae were not subjected to the same water c onditions as those in cages pl aced in the ballast tanks. Once the water entered the ballast tanks, there was li ttle water movement and the suspended sediment tended to settle to the bottom of th e barge with only a small amount of sediment going into each cage. The control cages in the river, however, had a constant s upply of new sediment that could enter the cage through the mesh and then settle out due to the decreased water movement with in the cage. Upon inspection, the higher sediment load in the control cages at both locat ions did not appear to a ffect larval Asian carp survival.

The number of larvae at the conclusion of each test inte rval for each set of three cages in the tanks or as controls are presented in Table 7 and Table 8. The tables show the number of each type of larvae that were alive and dead at th e end of each exposure interval. In addition, Table 7 tables present the percent of Asian carp th at were alive after these exposures. Larvae fr om all three cages in each tank were combined and are presented as the total for each exposure interval. Like wise, the numbers in Table 8 are the combined results from three control cages except as no ted. Asian carp survival rates du ring the study ranged from 92 percent to 100 percent in the ballast tank cages and 89 percen t to 100 percent in the c ontrol cages. The mean percent survival of Asian carp larv ae during all test inte rvals was 97.0 percent in the test cages and 97.6 percent in the control cages. Scanned laboratory datasheets for the survival experiments are presented in Volume II, Appendices B4, B5, B7, and B8.

During Trial 1, longer exposure interval s did not significantly d ecrease survival. The lowest survival was after a 48-hour exposure period. Prio r to pumping the water out of and th en refilling each of the ballast tanks during Trial 2, the test cages were removed from the tanks and a visual assessment was made of the status of the larvae in each cage. Larval survival was always high, therefore th e larvae were not removed from the cages. The cages were kept on the deck in the shade during pump out and flooding and were replaced into the ballast ta nks once the tank was refilled. This allowe d all caged larvae to be submerged in the tanks for about 144 hours. The average survival of Asian carp larvae was sli ghtly lower during Trial 2 (95.7 percent) than during Tria l 1 (98.3 percent), which might have been due to the longer exposure interval.

No statistical difference wa s found between survival of Asian carp la rvae in the test versus control cages during Trial 1 (P=0.622). Some larvae could not be identified to specie s, including one live larva that was damaged while being transferred to a storage vial at the end of the cage’s test interval ( Table 7) and a few dead larvae that had begun to deterior ate. The percent of Asian carp th at were alive in control cages after these exposures are presented in Table 8.

During Trial 2, the effect of highe r densities of larvae was assessed by stocking higher num bers of larvae into two control cages exposed for 119 and 120 hours. Su rvival at these higher de nsities was still high (94- 97 percent). No statistical difference was found between survival of larval Asian carp in the ballast tanks or in control cages during Trial 2 (P=0.2 93). No attempt was made to dete rmine the density of larval Asian carp in the river locations where the test larvae were collected.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

18 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 7. Ballast tank cages: number of alive an d dead Asian carp, non-Asian carp, and damaged larvae after varying exposure periods in cages during Trials 1 and 2. Th e percent alive is based on the number of larvae in the cages at the end of the exposure period. A blank cell means no larvae in that category were found.

Tank # Fill # Hrs Exposed Number in cages

after
exposure Asian Carp Larvae Non-Asian Carp Larvae Damaged Larvae Total Alive Total Dead Grand Total % Alive Total Alive Total Dead Total Alive Total Dead
Trial 1 1 1 8 97 39 2 41 95.1 52 4 2 1 24 90 24 24 100.0 57 9 3 1 48 85 30 2 32 93.8 40 13 4 1 72 65 26 26 100.0 26 12 1 4 2 72 80 66 66 100.0 2 3 9 3 2 94.5 76 32 32 100.0 16 18 10 2 2 117 77 39 39 100.0 28 7 1 2 1 2 132 82 36 1 37 97.3 39 6 Trial 1 Total 652 292 5 297 98.3 260 72 1 22 Trial 2 1 1 144 77 72 4 76 94.7 1 2 1 144 68 60 5 65 92.3 2 1 3 1 144 75 66 3 69 95.7 5 1 4 1 144 74 70 1 71 98.6 2 1 Trial 2 Total 294 268 13 281 95.4 10 2 0 1 Average for Asian carp per Trial 280 9 289 96.8Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

19 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 8. Control Cages: Number of alive and dead Asian carp, non-Asian carp, and damaged larvae after varying exposure periods duri ng Trials 1 and 2. The percent alive is based on the number of larvae in the cages at the end of the exposure peri od. A blank cell means no larvae in that category were found.

Hrs Exposed Number in cages

after
exposure Asian Carp Larvae Non-Asian Carp Larvae Damaged Larvae Total Alive Total Dead Grand Total % Alive Total Alive Total Dead Total Alive Total Dead
Trial 1 8 90 21 2 23 91.3 47 20 24 63 2 2 100.0 40 16 48 49 16 2 18 88.9 31 72 69 47 47 100.0 12 10 72 81 39 39 100.0 35 7 Total 352 125 4 129 96.9 165 53 0 0 Trial 2 143.5 89 87 1 88 98.9 1 143* 44 42 42 100.0 2 120** 54 34 1 35 97.1 7 10 2 119*** 63 31 2 33 93.9 12 18 Total 249 194 4 198 98.0 22 28 2 0 Average for Asian carp (both trials) 160 4 164 97.6 *began with three cages but one lost an entire mesh panel and many larvae lost so data from this cage was not presented **only one cage with 60 larvae at start ***only one cage with 90 larvae at start

Larvae from the tank and cont rol cages were preserved and later were classified by stage in the laboratory. Table 9 and Table 10 provide a summary of the number of a live and dead caged Asian carp larvae separated by their stag e. Asian carp placed in the cages in Trial 1 were genera lly younger (lower stage number) than those collected and used during the second trial for this study. During Trial 1, more larvae were stage 43, followed by stage 42, while during Trial 2, more larvae were stage 43 followed by stage 44. Based on Yi et al.’s (1988) description of larval stage and number of days post fertili zation, the majority of the larvae placed in the cages on 11 June for the first cage filling for Trial 1 were estimated to be about 11-13 days of age. This aligns with field observations of initial spawning by INHS personnel.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

20 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 9. Ballast tank cages: summar y of number of alive and dead Asian carp larvae of each stage for three test cages deployed for different exposure interv als during Trials 1 and 2. A blank cell means no larvae in that category were found.

Tank # Fill # Hours Exposed Number of Asian Carp Larvae by Stage Alive Dead 40 41 42 43 44 45 40 41 42 43 44 45
Trial 1 1 1 8 1 27 11 2 2 1 24 2 12 8 2 3 1 48 1 3 13 13 2 4 1 72 4 22 4 2 72 1 5 46 14 3 2 94.5 28 4 2 2 117 6 33 1 2 132 3 7 26 1 TOTAL 1 10 74 187 20 0 0 0 5 0 0 0 Trial 2 1 1 144 1 41 29 1 1 3 2 1 144 41 19 5 3 1 144 40 26 1 1 1 4 1 144 29 41 1 TOTAL 0 0 1 151 115 1 0 0 0 7 5 0

Table 10. Control cages: summary of number of alive and dead Asian carp larvae of each stage for three cages deployed for different exposure in tervals during Trials 1 and 2. A blank cell means no larvae in that category were found.

Hours Exposed Number of Asian Carp Larvae by Stage Alive Dead 40 41 42 43 44 45 40 41 42 43 44 45
Trial 1 8 10 10 1 2 25 1 1 65 5 11 1 1 72 4 38 5 118 7 31 1 TOTAL 0 11 27 81 6 0 0 1 3 0 0 0 Trial 2 143.5 1 22 63 1 1 143* 17 22 3 120** 2 15 16 1 119*** 10 21 1 1 TOTAL 0 0 3 65 106 20 0 0 1 2 1 0 *only two cages **only one cage with 60 larvae at start ***only one cage with 90 larvae at startAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

21 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Although nine different taxa of non-As ian carp larvae were used in surviv al experiments or collected in river sampling, no attempt was made to enumerate the numbe r of individuals of each of these taxa. A list of the non-Asian carp larvae collected dur ing this study is presented in Table 11.

Table 11. L ist of non-Asian carp larvae used in the su rvival experiments or coll ected during river sampling. Note the number of each of these larvae was not determined from either type of collection. Scientific Name Common Name Aplodinotus grunniens freshwater drum Carassius auratus* goldfish Catostomidae sucker Cyprinidae minnows and carps Cyprinus carpio common carp Dorosoma cepedianum gizzard shad Ictiobus spp. buffalo Morone spp. basses Notropsis hudsonius spottail shiner *Larvae might be a hybrid with the common carp.


### 4.3 Pump Effects Experiment Results A summary of the results from the four sets of trials conducted with two different diameter pumps and hoses is presented in Table 12. Only a few larvae (9 Asian carp plus 3 non-Asian carp) we re able to survive passage through either the 2- or 3-inch pum p and hos e. There was no significant difference between Asian carp survival after passage through the 2-inch or the 3-inch pump. Larvae collected for the pump experiments were darkly pigmented before passing th rough the pump but virtually colorless when removed from the plankton net (See Figure 5). The larvae that were actively swimming immediately after passage through the pump generally surviv ed and continued actively swimmi ng 30 minutes after pumping. This included both Asian carp and non-Asian carp ( Table 12). In contrast, thos e larv ae that were swimming erratically after passing through the pump were not alive 30 minutes afte r pumping. These results indicate that although some Asian carp larvae were able to survive ballast tank pumping, the total percentage of larvae that survived the pumping was extremely low.
No count was made of the number of larvae that pa ssed through the hose and pump for each of the pump tests. However, analysis of a photo of the larvae removed from the cod end estimated approximately 130 individual larva had been collected. For the twelve trials, only 9 Asian carp survived out of an estimated 1,560 (130 times 12) larvae sucked through the pump system for an estimated survival rate of 0.56 percent.
One approximately 3-inch long, relatively intact juve nile fish passed through the 3-inch pump and hose during one of the entrainment studies. It had been cu t into two main pieces plus a somewhat mangled center portion probably from contact with the pump impeller ( Figure 5).
During observations of norm al operations for pumping wa ter from ballast tanks on a barge in use on the river, it was noted that the discharge water is not generally pumped throug h a hose back to the river. Instead water exits the pump’s discharge port w ith some of it potentially hitting the metal deck of the barge before free-falling directly into the river. For the present testing, the discha rge water was pumped through a hose and discharged below water level into the collection net suspended in one of the barge spud wells. It is uncertain what additional mortality, if any, might ha ve taken place if the wate r had not been directed through the hose but had free-fallen 3-10 feet from the pump into the river surface. Likewise it is not possible to determine the effects of im pact with hose walls on the larvae.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

22 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 12. Summary of observations of larvae following passage through a 2-inch or 3-inch pump and hose. Observation were made immediately and 30 mi nutes after pumping. Test dates are in parentheses. Stage number refers to larval stage based on Chapman (2006). Pump Test Description of Larvae
Trial 1 2-inch pump (6/13/11) Run #1 All larvae were dead when initiall y removed from the pla nkton net cod end. Run #2 All larvae were dead when initiall y removed from the pla nkton net cod end. Run #3 Initially, two non-Asian carp were alive a nd actively swimming; all other larvae were dead. These two non-Asian carp larvae were still actively swimming after 30 minutes. Run #4 Initially, two Asian carp (s tage 43) were alive and actively swimming and one Asian carp (stage 43) was alive but swimming erratically; remaining larvae were dead. After 30 minutes, the actively swimming larvae were still actively swimming but the erratically swimming larva was no longer swimming and appeared dead. 3-inch pump (6/14/11) Run #1 All larvae were dead when initiall y removed from the pla nkton net cod end. Run #2 Initially, one Asian carp (stage 43) was alive and actively swimming; all other larvae were dead. After 30 minutes, the actively swimming Asian carp larvae was still actively swimming. Run #3 Initially, two Asian carp (stage 43) we re alive and actively swimming and two Asian carp (stage 43) were alive but swimming erratically; all other larvae were dead. After 30 minutes, the actively swimming larvae were still actively swimming but the erratically swimming larvae were no longer swimming and appeared dead. Trial 2 2-inch pump (6/19/11) Run #1 All larvae were dead when initiall y removed from the pla nkton net cod end. Run #2 All larvae were dead when initiall y removed from the pla nkton net cod end. Run #3 All larvae were dead when initiall y removed from the pla nkton net cod end. 3-inch pump (6/20/11)

Runs #1, 2, and 3 (combined*) Initially, four Asian carp (stage 44) and one non-Asian carp were alive and actively swimming; all other larvae were dead. After 30 minutes, the actively swimming larvae were still actively swimming. *larvae from these pump tests were accidently comb ined into a single vial in the field.



### 4.4 Water Quality Results Although some water quality da ta were collected during night hours, mo st water quality data were collected during daytime sampling events following the ballast ta nk filling and prior to tank pump-out. Illinois River temperature averaged 23.5 ° C (74.3 ° F), with a high of 25.6 ° C (78.1 ° F ) and a low of 22.0 ° C (71.6 ° F). Water temperatures in the ballast tanks of the expe rimental barge were similar to river conditions and averaged 23.4 ° C (74.1 ° F), with a high of 26.8 ° C (80.2 ° F) and a low of 22.3 ° C (72.1 ° F).

Dissolved oxygen concentrations in the Illinois Rive r averaged 5.30 mg/L, with a high of 5.51 mg/L and a low of 4.90 mg/l (Table 13; Figure 7). In contrast, ballast tank DO concentrations were m uch lower and more variable, averaging 2.9 mg/L. The highest DO concentration in the ballast tanks during Trials 1 and 2 was 5.51 mg/L, while the lowest DO concentration wa s 0.86 mg/L. Note that the single very high DO reading (6.55) in the ballast tank (Table 13) was likely an equipment or recorder error and was not included in Figure 7. In all tanks, DO decreased from concentratio ns near river values to as low as 0.86 mg/L over the 7 days trial periods.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

23 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 13. Summary of water qua lity measurement recorded dur ing Trials 1 and 2, June 2011.

Trial Tank Date Time (24-Hr Clock) Water Temperature (deg C) DO (mg/L) Conductivity (μS/cm) pH Total Ammonia

1 T1 6/11/11 9:55 25.2 6.55* 628 nc 0.25 1 T2 6/11/11 9:58 24.9 4.53 550 nc 0.25 1 T3 6/11/11 10:01 25.1 4.01 644 nc 0.25 1 T4 6/11/11 10:03 25.0 4.62 614 nc 0.25 1 R 6/11/11 10:10 25.0 4.99 650 nc 0.25 1 T1 6/11/11 17:25 26.8 4.30 637 nc 0.25 1 R 6/11/11 17:25 25.6 5.20 662 nc 0.25 1 T2 6/12/11 9:30 24.2 3.42 576 nc 0.50 1 R 6/12/11 9:30 24.4 5.23 666 nc 0.25 1 T3 6/13/11 9:30 24.1 1.87 612 nc 0.50 1 R 6/13/11 9:30 23.7 4.90 666 nc 0.25 1 T4 6/14/11 9:30 23.1 2.39 589 nc 0.50 1 R 6/14/11 9:30 23.1 5.07 644 nc 0.50 1 T1 6/15/11 9:00 22.4 2.74 610 nc 0.25 1 T2 6/15/11 9:03 22.3 2.50 606 nc 0.25 1 T3 6/15/11 9:06 22.3 2.55 628 nc 0.25 1 T4 6/15/11 9:08 22.5 2.80 613 nc 0.25 1 R 6/15/11 8:50 22.0 5.50 595 nc 0.25 1 T1 6/17/11 9:20 23.6 1.62 615 nc 0.25 1 T2 6/17/11 9:07 22.5 1.68 604 nc 0.25 1 T3 6/17/11 10:48 23.4 0.95 637 nc 0.25 1 T4 6/17/11 11:40 24.1 1.54 609 nc 0.25 1 R 6/17/11 9:05 22.5 5.42 534 nc 0.25 2 T1 6/18/11 10:28 22.8 5.51 546 nc 0.50 2 T2 6/18/11 10:30 22.8 4.70 549 nc 0.50 2 T3 6/18/11 10:32 22.7 4.87 552 nc 0.50 2 T4 6/18/11 10:35 22.7 5.31 546 nc 0.25 2 R 6/18/11 10:25 22.7 5.42 537 nc 0.50 2 T2 6/19/11 10:30 23.2 2.36 nc 7.0 0.50 2 R 6/19/11 10:15 23.0 5.27 nc 7.2 0.50 2 T3 6/20/11 10:45 23.0 3.09 540 nc 0.25 2 R 6/20/11 10:15 23.5 5.51 565 nc 0.25 2 T4 6/21/11 10:45 24.3 1.14 nc 7.6 0.25 2 R 6/21/11 10:30 24.3 5.39 nc 7.6 0.25 2 T1 6/24/11 11:45 22.6 1.14 nc 7.6 0.25 2 T2 6/24/11 9:45 22.2 1.23 nc 7.6 0.25 2 T3 6/24/11 9:55 22.4 0.86 nc 7.6 0.25 2 T4 6/24/11 11:25 22.6 1.15 nc 7.6 0.25 2 R 6/24/11 9:35 22.4 5.27 nc 7.6 0.50 T1, T2, T3, and T4 correspond to ballast tank numbers 1-4, respectively R = River C = Centigrade mg/L = milligrams per liter μS/cm = MicroSiemens per centimeter nc = not collected *possi ble equipment error; data not included in Figure 7Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

24 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure 7. DO concentration in Illin ois River and barge ballast tanks during Trial 1 (top) and Trial 2 (bottom). Note: T# refers to the ballast tank number.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

25 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Specific conductance (conductivity ) is a measure of the ability of water to conduct an el ectrical current. It is highly dependent on the amount of dissolved solids (suc h as salt) in the water (U.S. Geological Survey (USGS) website, accessed September 2011). Specifi c conductance is an important water quality measurement because it provides an idea of the amount of dissolved material in the water. Conductivity in the Illinois River averaged 613 micro-Siemens per centim eter (μS/cm), with a high of 666 μS/cm and a low of 534 μS/cm (Table 13). Conductiv ity in the ballast tanks during Tria ls 1 and 2 were similar to river conditions and averaged 595 μS/cm, with a hi gh of 644 μS/cm and a low of 540 μS/cm.

Due to equipment problems, pH was only measured during Trial 2. pH values in th e Illinois River averaged 7.5, with a high of 7.6 and low of 7.2 (Table 13). pH values in the ballast tanks closely tracked natural conditions in the Illinois river, with an average of 7.5, high of 7.6, and low of 7.0.

TAN values in the Illinois River and barge ballast tanks were steady, ranging between 0.25 and 0.50 mg/L. No differences in ammonia concentration were ob served between river and ballast tanks samples.



### 5 SURVIVABILITY TEST DISCUSSION/CONCLUSIONS

This study was conducted to determine if Asian carp eggs and larvae could 1) be entrained into barge ballast tanks, 2) survive while in these tanks, and 3) su rvive a single passage through a pump. The Study Plan (Test Plan in Volume II, Appendix A) proposed experi ments be conducted first in the river channel using Asian carp eggs and young larvae by keeping the barge pos itioned within the highest concentrations of these individuals during flooding of the ta nks while the larvae and possibly ne w eggs move down the river with the water current. After completing work in the river channel, the plan called fo r testing with older larvae from the same cohort while the experimental barge was positioned in quieter, slower moving waters. Because initial plankton tows in the river channel show ed that no eggs and low larval concentration were available, testing with the eggs and younger larvae in the study coul d not take place as planned. This was due primarily to a major Asian carp spawn occurring prio r to the initiation of these studies and apparently no subsequent spawning taking place in the area. Ther efore, no Asian carp eggs or larvae younger than about 11-13 days were available during these tests. By this stage, th e larvae had moved out of the main channel towards quieter water. Therefore the expe riments were conducted with the barge moored just outside the main channel.

Spawning of Asian carp is known to take place after the river water te mperature reaches 14-18 ° C along with an increasing river flow (Pa poulias et al., 2006; Jennings, 1988; De Grandchamp et al., 2007; Lohmeyer & Garvey, 2009; and Irons et al., 2011). Asian carp ha ve also been reported to spawn from as early as March until July (Papoulias et al., 2006) when river te mperatures and flow increase, typically from spring and early summer rains. Figure 8 presents the river gage height of the Illinois Rive r at Kingston Mines (about 7.4 m iles downriver from Pekin) and Copperas Cr eek (approximately 16 river miles south of Pekin) from early May through late August 2011. It also shows the 5-year averag e gage height for these locations between 2005 and 2010. As the figure shows, the Illinois River was in flood-like conditions during the time of this study, with gage heights nearly 6-8 feet over the 5-year (2005-2010) average. The study (shaded area) started shortly after the peak flow in late May. Based on the stage and assumed age (based on Yi et al., 1988) of the Asian carp collected during this study, it was estimated that these larvae were spawned during the period of increasing river (gage) height from May 25 until about June 3.Asian Carp Survivability Experiments and Water Transport Surveys inthe Illinois River, Volume I26UNCLAS//Public | CG-926 RDC |D. Heilprin et al PublicJanuary 2013Figure 8. Illinois River Gage Height at Kingston Mines and Copperas Creek. Theshaded area represents the time period that this study wasconducted. The average river gage heightfrom 2005-2010 for each location is also provided. Data provided by U.S. GeologicalSurvey, WRD, Urbana, IL.River Mile MarkersPekin – 152.9Kingston Mines – 145.4Copperas Creek – 136.8Havana - 120Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

27 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Papoulias et al. (2006) also stated th at Asian carp might be able to spawn more than one time per season. Alison Coulter (pers. co mm., September 2011; Coulter & Goforth, 2011) stated that during 2011 they found Asian carp eggs in the Wabash River from May th rough the end of July even though the river flow continued to decrease duri ng this period. They confirmed their vi sual field egg iden tifications with DNA analysis. Although there were small increases in the Illinois River flow during this study, secondary spawning did not appear to happen in the La Grange Reach as no eggs or younger larvae were found during any of the collections.

During the entrainment portion of these studies, it was dete rmined that larvae of a va riety of taxa could be entrained into the ballast tanks thr ough the experimental valves installe d in the tanks. The 3-inch valve penetrations were intended to simulate a break in a ta nk seam or a hole in the barg e hull. Discussions with barge operators and Coast Guard personnel indicated that the 3-inch opening was considerably larger than most cracks occurring during normal barge operations. A hol e as large as 3 inches would fill a tank to about 50 inches in approximately 90 minutes and would probably be noticed by th e crew as a change in handling or during inspections. However, if the penetrations do repr esent naturally occurring breaks in barge walls, then fish larvae could be entrained into ballast tanks. It is expected that if eggs had been available and had been entrained from the swift moving cu rrent, they would settle to the bott om of the quiet water in the tanks and survival would be low. Thus there is some potenti al, though very slight, that eggs or larvae could be inadvertently moved across the barriers during normal barge operations in a damaged barge. It is important to note that this would occur only during the spawning season (late spring – summer).

The pump effects experiments showed that if Asian ca rp larvae were entrained and then pumped out, only a very small percentage of them (0.56 percent) could su rvive being pumped through either a 2-inch or 3-inch pump. If tanks were actively ballasted (i.e., pumped) and then deballasted, it is highly unlikely that larvae could survive two passages through a pump. This could be readily tested by using the methods of the pump experiments of this study and collecting the pumped wate r and larvae in a portable tank after the first pass. The larvae and tank water could be pumped once more and collected in a submerged plankton net. Active swimming of larvae for more than 30 minutes would indi cate survival potential. A barge or barge-size tank would not be necessary since the volume of water pumpe d would be small. During the pump effects testing, a hose was used to pump ballast water from the tanks directly into a suspended plankton net. This is different from normal barge operations when no hose is used on the pump’s discharge. These studies could not determine whether Asian carp la rvae that survived passage through a pump would then survive free fall into the river if a discharge hose were not used.

Kolar et al. (2007) and King et al. (20 10) stated that bighead and silver ca rp have hybridized in the wild and produced fertile offspring. Blake Ruebush (pers. comm., June 2011, Illinois River Biological Station, Havana, IL) stated that many hybrids of bighead and silver carp have been colle cted during the Long Term Resources Monitoring Program (LTRMP) in the La Gra nge Reach of the Illinois River. All individuals referred to in this report as larval Asian carp visually appeared to be silver carp based on figures presented in Yi et al. (1988) and Chapman & Ge orge (2011), but some might have been hybrids that showed the same pigmentation patterns characteristic of silver carp.

The original survival study plan (See Volume II. Appendix A) called for fish in early developmental stages to be suspended in cages in the ba llast tanks to document whether larv al Asian carp could survive for a maximum of 108 hours in a ballast tank. Based on high (98%) larval survival in Trial 1 (up to 132 hours exposure), all Trial 2 exposur e intervals were increased to 144 hours for all tanks . A sian carp larvae inAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

28 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Trial 2 also had a high (95.7%) survival rate suggesting that entrained la rvae could survive in tanks for at least 6 days. The average Asian carp (stages 40-45) survival in the cont rol cages during both trials was also high (97.7%).

Water quality parameters measured during the trials show ed that Asian carp larvae were able to withstand a wide range of the measured water quality parameters , including temperature and DO. The most variable water quality parameter was DO, which decreased to a le vel of 0.86 mg/L inside one of the ballast tanks but was not low enough to substantially affect survival. It has been reported that grass, bighead, and silver carp can tolerate oxygen levels as low as 0.5 mg/l (Ore gon Sea Grant website, accessed August 2011). In addition, Jennings (1988) reported th at juvenile bighead carp (avera ge weight = 73 mg) survived DO concentrations of 0.33 mg/L, while smaller indi viduals (average weight = 23 mg) survived DO concentrations of about 0.40 mg/L. Although the current study used younge r individuals, it appears that Asian carp larvae collected in the Il linois River are also able to tole rate low DO levels. Because the DO concentrations in ballast tanks vary, even on a single barge, the actual tolerance level of Asian carp egg and larvae for low DO is not necessary for assessing survival during normal barge operations.
Other investigations related to the potential transpor t of Asian carp early life st ages include surveys to determine the amount of water carr ied by barges and towboats. A 2010 survey of 969 ballast tanks on 132 barges and 14 towboats found measurable water in only 5.2 percent (50 tanks) of ta nks. With the exception of three tanks with depths over 36 inches, the average dept h of those tanks with water was 7.2 inches (Heilprin et al, 2011). A similar survey was conducted fo r barges operating in the im mediate vicinity of the USACE dispersal barriers in 2012. Tanks on 39 barges a nd 2 towboats were checked with only 6 percent of the tanks containing measurable wa ter. Heilprin et al (2011, updated October 2012 (See Sections 7-12 of this report) provide a description of barges and the standard opera ting procedures used by towboats operators. Operators interviewed du ring the 2012 survey also indicated they used these procedures. The ballasting procedures call for the us e of portable pumps on the rare occasions when ballasting is necessary.
A final question is whether Asian carp eggs or larvae th at were present in barge or towboat ballast tanks could survive passage across the USACE electrical barriers. When a ba rge or towboat passes through the electrical field generated by the disp ersal barriers, the hull acts as a Fara day cage to prevent any electrical potential from crossing into a partially filled tank (Dr. N. Yankielun, pers. comm., October 2012). Essentially the electrical current s in the canal water are conducted along the highly conductive metal hull without creating a potential differen ce anywhere along that hull. Since th ere is no potential difference, the current cannot pass into the tanks. Thus the hull effectively insolates the ballast water from the electrical field, so any eggs or larvae presen t in the ballast water would not be affected by transiting across the barriers.
In summary, this study showed that Asian carp larvae could potentially be entrained into ballast tanks through hull cracks and can survive the conditions in these tanks for up to 144 hours. But, if these larvae are then pumped out of the tanks us ing standard commercial methods, thei r survival rate is extremely low (less than 1 percent). Survival following intentional ballasting and de ballasting (i.e., two passages through a pump) is even less likely. Finally, it is important to note that the poten tial transport of eggs and larvae would occur only during the spawning season (late spri ng – summer). Based on the results of this study, it is concluded that the risk of transport of eggs or viable larvae through barg e ballast tanks is very low. W hile additional research of this matter may be unde rtaken, it is not warranted at this time.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

29 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 

### 6 WATER TRANSPORT DURING NORMAL OPERA TIONS OF TOWBOATS AND BARGES IN THE ILLINOIS RIVER

The United States Coast Guard (USCG) is tasked by the National Aquatic Nuisance Prevention and Control Act (1990) and National Invasive Speci es Act (1996) with eliminating as much as possible the introduction of non-indigenous species (NIS) via ba llast water. Under the Great La kes Restoration Initiative (GLRI) Act, the Coast Guard has been funded by the U.S. E nvironmental Protection Agency (EPA) to investigate the potential for towboats and barges to transport Asian carp and other species within the Chicago Shipping and Sanitary Canal (CSSC) and across the electrical dispersal barrier cons tructed by the U. S. Army Corps of Engineers (USACE) as a preventive measure to keep fi sh from migrating into and out of the Great Lakes.

Ballast tanks and voids of towboats and barges were initially investig ated in August 2010 to determine the volume of water being transported across the U. S. Army Corps of Engineers’ electr ic dispersal barriers in the Chicago Sanitary and Ship Canal. A second survey of ballast tanks of barges and towboats operating in the immediate vicinity of the Barriers was conducte d in July – August 2012. Results from those surveys were initially presented in the repo rt “Water Transport During Normal Operations of Towboats and Barges in the Illinois River” updated in October 2012. The main body of that report is presented here as a supplement to “Survivability of Asian Carp in Barge tanks in the Illinois River”. Data sheets from this effort are available in Volume II, Appendixes C and D, of this report.

Results of a second survey conducte d on barges operating around and across the barrier are included as an update in Section 12 of this report.



### 7 SURVEY METHODS

Visual inspections of barge and towboat ballast ta nks and voids (hereafter called ballast tanks) were conducted along the CSSC in the vicinity of Lemont, IL ( Figure 9) between 18 and 26 August 2010. Barge

com panies in this area were contacted for access to availa ble barges and towboats. In addition to barges in the Lemont, Channahon, and Romeoville areas, barges owned and operated by Ki ndra Lake Towing (KLT) in Chicago were also inspected. KLT occasionall y moves barges/towboats through the Lemont area. Table 14 indicates the locations of ve ssels inspected during this study.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

30 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Chicago River

Chicago Lock North Shore Channel Wilmette Sluice Gate

Calumet River

Indiana Harbor

Burns Harbor O’Brien Lock-and-DamIllinoisIndiana L i t t l e C

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# Lake Michigan CSSC Sampling locations

Romeoville

Channahon Lemont

Figure 9. Barge and towboat ballas t tank sampling locations (red dots ) along CSSC (red line), August 2010.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

31 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 14. Vessel company and location of barge and towboat sampling, August 2010.

Vessel Com p an y Location American River Trans p ortation Com p an y ( ARTCO ) ARTCO Shi py ard ( Lemont ) Illinois Marine Towin g ( IMT ) Channahon, IL IMT Herita g e Stora g e Yard IMT IMT ( Channahon ) IMT Lemont, IL American Commercial Lines Lemont, IL ARTCO Lemont, IL ARTCO Morris, IL ARTCO Ottawa, IL ARTCO Power Plant ( Romeoville, IL ) Hanson Material Services Romeoville, IL Hanson Material Services Romeoville/Loadin g area ARTCO Seneca, IL Kindra Lake Towin g South Chica g o, IL Kindra Lake Towin g Walsh Sli p ( Chica g o, IL )

Ballast tanks from barges, either rafted along the rive r bank or at the operator’s shipyards, were visually inspected for the presence of condensation and/or m easureable amounts of water. Three types of barges were sampled: tank barges, deck barges, and hopper barges. The number of balla st tanks per barge ranged from 6 to 18, depending on the type of barge. Tank barg es had separate port and starboard wing tanks with a bow and stern tank, while deck barges had separate po rt and starboard tanks sepa rated by a bulkhead at the keel creating separate port/s tarboard ballast tanks. Several of the deck barges were found to have port, center, and starboard tanks per section resulting in a high number of ballas t tanks present. Ballast tanks ran side to side on hopper barges.
Data for all barges and towboats insp ected was recorded on data sheets in the field. Data included: barge type, number of ballast tanks, location of ballast tank inspected, hatch type and condition, condensation presence, and whether hatch was sealed. Water depth wa s determined using a weighted measuring tape that was lowered into the ballast tank. Depth was determined from deck level by measuring the point where the weight was observed hitting the surface of the water to the point the weight reached bottom of the tank. If the water level in an individual ballast tank was measurable (defined as greater than or equal to 2 inches, as determined with a tape measure), a Yellow Springs Instrument (YSI) Model 550A Temperature-Dissolved Oxygen probe was then used to record temperature and dissolved oxygen (DO).
Information regarding the number of barges and towboats passing thr ough the CSSC annually (2007 data) was also obtained from the USACE a nd is presented below in Section 9.4. This information does not necessarily reflect th e actual number of vessels transiting over the disper sal barrier as some local vessels cross the barrier but do not transit the full CSSC.



### 8 SURVEY RESULTS

8.1 Water Depth

8.1.1 Barges
Between August 16 and 25, 2010 a total of 969 individual ballast tanks were inspected on 132 barges and 14 towboats. Data obtained during the survey is described in Appendix E and provided as a tab le in Volume II. The photographs in Appendix C show several barge t ypes, hatch arrangements, and clearances underAsian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

32 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 bridges. Barges sampled included 19 deck barges, 99 hopper barges, and 14 tank barges. Of the 969 ballast tanks inspected, 50 ballast tanks (5.2 pe rcent), all of which were on barges, had measurable levels of water. Water depths in these ballast tanks ranged from a low of 2 inches to a high of 117 inches ( Figure 10). Water depth in deck barges ranged between 4.5 – 24 inches, while hopper barges ranged between 2 – 117 inches (F igure 10). No measurable water was found on tank barges.

For all barges with measurable water in their ballas t tanks, overall average water depth was just over 11.0 inches. When three hopper barge tanks with depths of 42, 76, and 117 inches are excluded, water depth averaged 7.2 inches. No information was availabl e to determine why these th ree tanks contained more water than the remaining 47 tanks with measurable water.

B B B B

B B B B B B B B B B B J J

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0 1 2 3 4 5 6 7Water Depth (cm) / Temperature (deg F)Dissolved Oxygen Concentration (mg/L) B Water Depth (in) J Temperature (deg F) H DO (mg/L)

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0 1 2 3 4 5 6 7 8Water Depth (cm) / Temperature (deg F)Dissolved Oxygen Concentration (mg/L)

Figure 10. Water depth (inches), wate r temperature (°F), and DO concentra tion (mg/L) for deck barge (top) and hopper barge (bottom) ballast tanks sampled in August 2010.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

33 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 8.1.2 Towboats

Of the 14 towboats inspected in A ugust 2010, only four had m easurable amounts of water in their ballast tanks. Water depths for towboats ranged from a low of 18 inches to a high of 72 inches (Figure 11). Average water depth for towboats was 50.5 inches. No information regarding the ballasting of the towboats was available. Towboat operato rs indicated that ballasting is not routinely conducted.

B B B B J J J J H

H

H

H

Stacey Dianne Harvest Run MORGAN OLD MISSION 0 20 40 60 80 100 120

0 1 2 3 4 5 6DO Concentration (mg/L) B Water Ddepth (in) J Temperature (deg F) H DO (mg/L)

Figure 11. Water depth (inches), wate r temperature (°F), and DO concentr ation (mg/L) for towboat ballast tanks sampled in August 2010.



### 8.2 Water Temperature

8.2.1 Barges

Water tem perature (°F) was recorded for the 50 barge ballast tanks that contai ned measurable amounts of water and ranged between 69.5 - 86.7 °F. Water temperature was relatively consistent in most ballast tanks. For deck barges, water temperature ranged between 69.5 - 83.4 °F, with an average of 71.5 °F (Figure 10). Water temperatures for hopper barges ranged between 70.4 - 86.7 °F ( Figure ). Average water temperature in hopper barges was 80.0 °F. For all hopper and deck barges com bined, average water temperature was 77.4 °F. As mentioned above, there was no measurable water in tank barges.

8.2.2 Towboats

Water tem perature in towboat ballas t tanks ranged between 69.2 - 84.5 °F ( Figure ) with higher
tem peratures found in those tanks with less water. Similarly, the ballast tank with the most water (72 inches) had the lowest temperatur e. Average water temperature in towboat ballast tanks was 76.2 °F.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

34 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 8.2.3 Ambient Conditions

Am bient water temperature was measured at approxima tely 1 meter depth alongside the barges twice during the survey. Water temperature at the IMT Ch annahon facility on August 17 was 86.3 °F. Water temperature of 70.0 °F was recorded at the Kindra Lake Towing facili ty near Chicago on August 19. In general, one can expect higher water temperatures in summer in the Lemont area (average = 80 °F) than in those areas closer to Lake Mi chigan (average = 71.5 °F).



### 8.3 Dissolved Oxygen (DO)

8.3.1 Barges

DO concentrations in the water of the 50 ballast tanks ranged between 0.44 - 7.80 m g/L and were highly variable. For deck barges, DO ranged between 3. 60 - 6.17 mg/L, with an average of 5.29 mg/L ( Figure ). For hopper barges DO concentrations ranged between 0.44 - 7.80 m g/L. Average DO in hopper barges was 3.98 mg/L. No apparent trends in DO concentration were observed between ba rges with more water compared to those with less water in their ballast tanks. Tank barges did not contain sufficient water to measure DO. This is not unexpected since tank barg es are inspected by Coast Guard after collisions, elisions, etc and, if leaks are found, are re moved from service until repairs are made.

8.3.2 Towboats

DO concentrations in ballast water from towboats ranged from 2.54 - 5.10 mg/L (Figure 10). Average DO in towboats was 3.65 mg/L.

8.3.3 Ambient Conditions

As was done with water tem perature, ambient DO was also measured twic e during the survey at approximately 1 meter depth alongside the barge. DO at the IMT Channahon facility on August 17 was 2.76 mg/L, while DO at the Kindra Lake Towing facility near Chicago on August 19 was 8.46 mg/L. This large difference could potentially be an artifact of sampling since the K LT sample was taken in the morning and the IMT sample was recorded in the afternoon.



### 8.4 Vessel Traffic

Annual vessel traffic across the electric fish barrie r was obtained from USACE for 2007 and is presented in Figure 12. A total of 5,792 vessels crossed the electric fish barrier in Rom eoville in 2007, including 2,246 liquid hazardous cargo barges, 2,650 commercial vessels, a nd 896 recreational vessels. A variety of barge types cross the electric barrier each year, including hopper, tank, and deck barges similar to those inspected in this study.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

35 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure 12. Annual vessel traffic across the elec tric fish barrier in 2007 (Source USACE/OMNI).



### 9 SURVEY DISCUSSION/CONCLUSIONS

It is widely accepted that water temperature, flow conditions, food, and preda tion are important factors influencing larval survival and grow th. Little information exists on water quality tolerances such as temperature and DO for Asian carp, esp ecially the early stages of devel opment such as eggs and larvae. Most of the existing litera ture focuses on common carp ( Cyprinus carpio L) and grass carp ( Ctenopharyngodon idella ), which is also an Asian carp species.

Opuszynski et al. (1989) found the upper lethal temperature (ULT) for silver carp was 43.5 - 46.5 °C (110- 116 °F) and also found no significant differences in survival of fish reared at lowe r and higher temperatures when proper food was used. Golovanov and Smirnov (2007) used the chronic leth al impact method with a water heating rate of 1 °C/day or 0.04 °C/h (1.8 °F/day or 0.07 °F/hr) to determine ULT for common carp. At 1 °C/day, (1.8 °F/day) fish successfully acclimated to temperature increases.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

36 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Silver carp are also quite tolerant to low water temp eratures and have been re ported to feed at water temperatures of 10 to 19°C (50-66 °F) in Israel (Lev enter 1979, cited in Wrigley et al. 1988). When the water temperature dropped below 15°C (59 °F), the appe tite of silver carp was reduced, and below 8-10°C (46-50 °F), feeding almost ceased (FAO 1980; Tripathi 1989). At water temperatures below 18°C (64 °F) or higher than 31°C (88 °F), rates of ovulation and hatching of silver carp ha ve been reported to be low with high rates of abnormal embryonic development (FAO 1980).

Grass carp tolerate a wide range of water temperatures from 0 - 33 °C (32 - 91 °F), with temperatures greater than 38 °C (100 °F) being le thal for adults (Fedorenko and Fras er, 1978). ULT for grass carp fry ranges from 33 - 41 °C (91 - 106 °F), and for yearlings the range is 35 - 36 °C (95-97 °F), depending on season (Chilton and Muoneke, 1992). Grass carp also a ppear to tolerate moderately rapid changes in temperature. Shireman and Smith (1983) found finger lings (5 - 7 cm or 2 - 3 inches) could tolerate temperature increases from 4 - 22 °C (39 - 72 °F) ove r a relatively short amount of time (~2 - 3 hours).

Bighead carp can tolerate extremes in water temperature, from cold temperate to tropical (Kolar, et al. 2007). In their native range in China, bighead carp can spawn at temperatures as low as 18°C (64 °F) in the Han River (Chunsheng, et al. 1980). Negonovskaya ( 1980) reported bighead car p fingerlings feeding activity continued at 10 °C (50 °F ) in lakes in Russia's Pskov Regi on, but most active feeding activity occurs at 20 - 22 °C (68 - 72 °F). Experiments with thermal preferences conducted in Texas (Bettoli, et al. 1985) indicated that young bighead carp (56 - 73 mm) acclimated to temperatures at 23.0 oC (73 °F), selected a mean temperature of 25.4 oC (78 °F), and ha d their critical th ermal maximum at 38.8 oC (102 °F). Although little information exists on lo wer water temperature lethal limits for this species, the presence of bighead carp in rivers and reservoirs in the Manchurian Plain that remain frozen 4 to 6 months out of the year suggests that the species is quite cold tolerant.

The vast majority of ballast tanks sampled (919 of 969; 95 percent) were dry; water in the ballast tanks sampled in the current study had littl e variability in temperature and ranged between approximately 70 - 87 °F (21 - 30 °C) which was similar to the two ambi ent river temperatures measured. Comparison of temperature ranges in the barge ballast tanks with values found in the lit erature suggests that Asian carp larvae could potentially tolerate the range of temperat ures found in the tanks and probably the fairly rapid changes in temperature in both th e barge and towboat ballast tanks.

Information on carp (adult, juvenile, or larvae) tolerances to DO is extremely limited. Cudmore and Mandrak (2004) found DO levels below 3 mg/L can cause stress in grass carp. Shireman and Smith (1983) reported that the same species could tolerate oxygen concentrations as low as 0.2 mg/L and fingerlings survived in DO levels between 0.41 - 28 mg/L. Other studies have also found that young grass carp are more susceptible to low oxygen concentr ations than older fishes and that vulnerability varies with season (Chilton and Muoneke, 1992) with le ss tolerance for lower DO concentra tions in colder (winter) water compared to more tolerance for lowe r DO concentrations in summer, when the waters are warmer (Versar, Inc., 1999).

DO concentrations in barge and towboat ballast ta nks measured in this study ranged between 0.44 - 7.80 mg/L. This is well within the ra nge of tolerances for other carp sp ecies and is likely within the range for bighead and silver carp. This suggests that water quality conditi ons with ballast tanks, although not optimal, could support early lif e stages of Asian carp.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

37 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Of the 969 tanks and voids inspected during August 2010, 50 barge tanks (5.2 percent) had measurable levels of water. In April 2010, a visual ballast tank inspection was conducted (no water quality sampling) and found that of the 127 ballast ta nks inspected, only 4 tanks (3.1 percent) had greater than approximately 3 inches of water (visual inspection). A worst case scenario can be constructed by assuming that no barge has more than one ballast tank with measurable water (2 inches or more) and that the roughly 5 percent rate for tanks with measurable water is representative. Applying this 5 perc ent rate to the USACE 2007 data for the number of cargo vessels passing thr ough the electric barrier (see Section 9.4), it can be estimated that approxim ately 112 barges with water in their ballast tanks could potentially pass through the electric barrier each year.



### 10 SURVEY RECOMMENDATIONS

Information collected during this study is useful in understanding volumes and water quality conditions in barge and towboat ballast tanks and voids and is useful as a baseline for the development of additional studies to test whether ballast tanks are a viable vector for Asian ca rp transport. Although the existing literature is sparse on the toleranc es of Asian carp, information on othe r species of carp suggests these species would be able to tolerate a wide range of water quality conditi ons, including high water temperature and low DO concentrations. It must be noted that the current study only provi des a “snapshot” of what ballast water conditions are during a summer event and that no barge hi story was recorded for those tanks that contained substantial amounts of water. It is possible that those barges had leaks and had remained rafted in place rather than being kept in use. A dditional sampling events and barge information during other time periods may be warranted to look at possible s easonal variability within barge and towboat ballast tanks and voids.

Larval fish survivability within a barge or towboat ba llast tank has never been studied. In determining whether ballast tanks are to be consid ered a possible vector for larval tran sport, the next step should be to determine whether early developmental stages of Asian car p (eggs and larvae) are able to tolerate/survive in these tanks. In addition, other studies should be performed to help evaluate transport mechanisms for Asian carp. These should include sampling barge tanks for di fferent life stages of As ian carp, evaluating the effects that tank leakage has on the potential trans port of Asian carp, and inve stigating the effect of deliberate ballasting via pu mps on early life stages.



### 11 UPDATE FROM 2012 SURVEY OF LOCAL BARGES

The November 2010 Towboat/Barge Sampling Study focused on large barge companies that move hundreds of barges across the electric dispersa l barrier. The July 2012 survey addre ssed localized barges and towboats that regularly work within 10 miles of and regularly transit over the USACE di spersal barrier. The study examined the condition and amount of measurable wate r found in ballast tanks of those local vessels.

Initially, the intent was to shift from the large barg e companies previously surveyed to local/small barge providers. Research revealed there are no small companies that move barges within the dispersal barrier focus area, but a few of the large companies previously visited do designate some of their barges and tow boats to work in the local barrie r area. Therefore the inspection team focused on these local groups of barges operating in the immediate vici nity of the dispersal barrier.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

38 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 

### 11.1 Methods Used in 2012 Survey

The methods used in the previous survey were again used in this survey. Loca lly operating barge companies provided towboat transportati on for the inspection team to locally operating barges along the CSSC in the vicinity of Lemont and Romeoville, IL (Table 15) between 30 July and 1 August 2012. Barges were located near the USACE electrical dispersal barrier indicated in Figure 9.

Table 15. Vessel Company and Location of Barge and Towboat Sampled, July-August 2012. Vessel Company Location
IMT Lemont, IL ARTCO Romeoville, IL (Power Plant) Midwest Generation, LLC Romeoville, IL (Power Plant) Hanson Material Services Romeoville, IL (Loading area)

Ballast tanks of barges either raft ed along the river bank or at the opera tor’s facilities on the CSSC were visually inspected for the presence of condensation and/or measureable amounts of water. Hopper barges were the only type of barges avai lable during the inspections. Cargos were either construction materials (sand, gravel) or coal. The barges inspected were double-hull construc tion with single or double-raked ends. On most of the barges ballast tanks run from si de-to-side without obstructi ons. Eleven older barges had a bulkhead in the center which se parated port and starboard tanks. Ba rges additionally had a stern tank, bow tank, or both which ran from side to side. The number of tanks per barge ranged from 6 to 13 depending on the construction, altho ugh 70 percent had 10 tanks each.

Data for all barges and towboats inspected was reco rded on data sheets in th e field, which were then transferred to the tables included in Volume II, Appendix E. Barge location, barge type, number of tanks, location of tank inspected, condensati on/water presence, and if hatch wa s found sealed were recorded for each barge inspected. If water was present in a tank, a sounding device (tape me asure/sounding stick/string and weight) was used to determine if the water level was greater than 4 inches. Four inches of water allowed use of a Yellow Springs Instrument (YSI) Model 550A Temperature-Dissolved Oxygen probe to measure temperature and dissolved oxygen (DO). Ambient river temperature and DO in the vicinity of the barges were also recorded twice a day for a base line comparison. Photographs of tanks and barge configurations seen during the survey are provided in Appendix D.



### 11.2 Results of 2012 Survey

Only two towboats were available for transportation a nd inspection. Both crews re ported that they rarely adjust the ballast water in their tanks. A crewma n from the towboat STACY DIANE (Hanson Material Services) reported the ballast water in the towb oat stern tank was at least 4-6 months old.

Of the 39 barges inspected, the majority were in gene rally good condition. Most were empty and all were rafted along various portions of th e CSSC. Barge ballast tanks were typically accessib le through a hinged manhole type hatch (Figure 13) with a rubber seal and e dge hatch dogs to latch secu rely. All hatches were closed but none were found latched. A few barges us ed a round removable tank ma nhole cover (Figure 14). A small number of these covers were found loosely sitt ing over the access hole and not mated tightly closed.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

39 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure 13. Ballast tank hinged manhole hatch.

Figure 14. Ballast tank re movable manhole cover.

Some barges also incorporated 7-inch tank access t ubes (Figure 15). This type of access tube was not reported in the 2010 survey and appear ed to be on older barges. These tubes are covered with a hinged cap and are used to conveniently insert pump hoses when needed. The barge crewmen stated these tubes were used as a primary access to the tank when sounding or when pumping into/out of the tanks. Because the crewmen prefer to regularly use these smaller tank acces ses, many of the associated larger tank hatch covers were rusted shut from lack of use. The inspectio n team was able to evaluate the tank water level and perform measurements using these tube accesses.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

40 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure 15. Ballast tank access tubes.

Barge ballast tanks were approximate ly 10 feet deep. Typically only a small amount of water was found in the tank and the bottom 4-inch cross-memb ers were usually visible (Figure 16).

Figure 16. Typical ta nk inspection view.

11.2.1 Water Depth in Tanks from 2012 Survey

A total of 28 8 barge tanks were inspected; however, three hatches were not accessible due to covers that could not be opened for inspection resulting in 285 barge tanks evaluated for water content

. As shown in Table 16, results of the barge inspection indicate d 44 percent of all tanks were completely dry and 50 percent had only a trace amount of water. Only 6 percent of the tanks held water above the 4-inch measurable threshold that allo wed the use of the YSI meter.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

41 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Table 16. Barge Tank Water Content, July-August 2012.

Number of Tanks Percent of Total Tanks Dry 126 44%
Less than 4" 141 50% Equal to or greater than 4" 18 6%
Total 285 100%

Of the two towboats accessible to inspect, both ha d a bow and stern tank. The STACY DIANE (Hanson Material Services) had an empty bow tank and held 32 inches of ballast wate r in the stern tank. The WINDY CITY (Illinois Marine Towing) had an empty stern tank and held 24 inches of ballast water in the bow tank.

11.2.2 Water Temperatures and Dissolved Oxygen from 2012 Survey

Water tem perature in barge and towboat tanks range d between 81.1 - 86 °F. DO in tanks ranged between 2.85 - 7.07 milligram per liter (mg/L) (Figure 17). Ta nk water in the STACY DIANE was 90 °F and had a DO reading of 3.11 mg/L when measured during mid- morning. The WINDY CITY bow had a temperature of 84.5 °F and DO was 6.65 mg/L when the water wa s measured in the afternoon. River baseline measurements of temperature and DO were 82.1 to 90 °F and 3.11 to 7.55 mg/L, respectively.

Figure 17. Water Temperature a nd Dissolved Oxygen Comparisons.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

42 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 

### 11.3 Conclusions from 2012 Survey

The overall findings of this survey of local barge tra ffic near the dispersal barrier compares closely to the condition and water content/levels of the larger initial barg e study of 2010. The perc entage of tanks with measurable amounts of water (5 percent versus 6 per cent) and the temperature and DO in those tanks are very similar. Due to lack of ava ilability, only two tow boats were inspect ed. Each tow boat contained 2 feet or more of ballast tank water; at least one tank had been filled for several months. Because towboat tanks are actively filled more frequently than barge tanks, additional tow boat inspections may be warranted. Due to the extended period of time the towboat tanks remain filled, long-term survival of Asian carp larvae in them is unlikely.

The team was able to collect data on 39 barges rafted along areas of the river. The barges inspected were usually empty and in generally good condition. One crewmember co mmented that most barges sit uncovered the majority of the time so there is a potential for rainwater to seep through small weld cracks or holes contributing to the trace amou nts of water that were found. One barge manager reported that the empty barges moored along the river are not checked regularly for water in ballast tanks, but are inspected anytime they are moved. He said barges are also monitored for water in the ballast tanks every 6 hours while in use, which is the same schedule report ed by operators of longer distance barges in 2010.

The barge companies have experienced a recent slowdow n in activity around the dispersal barrier focus area and consequently fewer barges/tugboa ts were available for access. A local barge company representative from the area mentioned that his company was one of the main barge providers th at traverse the dispersal barrier. He informed the team that regular coal deliveries will end by mid-September 2012, and his barges will no longer be used to transport coal through the ba rrier and up to the Chicago area. This slowdown will dramatically reduce the total number of barges that cross through th e dispersal barrier.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### 12 REFERENCES

12.1 References for “Survivability of Asian Carp in Barge Tank s in the Illinois River” Chapman, D. C. & George, A. E. 2011. Developmental Rate and Behavior of Early Life Stages of Bighead Carp and Silver Carp. US Department of th e Interior, US Geological Society Scientific Investigations Report 2011-5076.
Chapman, D. C. & Hoff, M. H. 2011. Introduction. Inva sive Asian Carps in North America. American Fisheries Society Symposium 74:1-3.
Chapman, D. C., ed. 2006. Early Development of Four C yprinds Native to the Yangtze River, China. U.S. Geological Survey Data Series 239, 51 pp.
Coulter, A. & Goforth, R. R. 2011. Silver and Bighead Carp Movements and Habitat Use in the Wabash River, IN. American Fisheries Society Conference. Abstract.
Cudmore, B. & Mandrak, N. E. 2011. Assessing the bi ological risk of Asian carps to Canada. American Fisheries Society Symposium 74:15-30.
DeGrandchamp, K. L., Garvey, J. E., & Csoboth, L. A. 2007. Linking adult reproduc tion and larval density of invasive carp in a large ri ver. Transactions of the Amer ican Fisheries Society 136:1327-1334.

Irons, K. S., Sass, G. G., McClelland, M. A., & O’ Hara, T. M. 2011. Bigheaded carp invasion of the La Grange Reach of the Illinois River: Insight fr om the Long Term Monitoring Program. American Fisheries Society Symposium 74:31-50.
Jennings, D. P. 1988. Bighead carp ( Hypophthalmichthys nobilis ): Biological Synopsis. US Fish Wild. Serv. Biol. Rep. 88(2). 25 pp.

Jerde, C. L., Mahon, A. R., Chadderton, W. L., & L odge, D. M. 2011. “Sight-unseen” detection of rare aquatic species using environmental DNA. Conservation Le tters. 4:150-157.
Kelly, A. M., Engle, C. R., Armstrong, M. L., Freeze, M., & Mitchell, A. J. 2011. History of Introductions and Governmental Involvement in Promoting the us e of Grass, Silver and Bighead Carp. American Fisheries Society Symposium 74:163-174.
King, T. L., Eackles, M. S., & Chapman, D. C. 2010. Tools for assessing kinshi p, population structure, phylogeography, and interspecific hybr idization in Asian carps invasi ve to the Mississippi River, USA: isolation and characterizati on of novel tetranucleotide microsat ellite DNA loci in silver carp Hypophthalmichthys molitrix . Conservation Genetics Resources 3(3) 397-401.
Kolar, C. S., Chapman, D. C., Courtenay, W. R., H ousel, C. M., Williams, J. D., & Jennings, D. P. 2007. Bigheaded carps: a biological synopsis and environmental rick assessment. American Fisheries Society, Special Publication 33, Bethesda, Maryland.
Lohmeyer, A. M. & Garvey, J. E. 2009. Placing the No rth American invasion of Asian carp in a spatially explicit context. Biol. Invasions.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

44 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Martin, B. Asian carp threat imminent. Leader & Kalkaskian News (accessed September 2011 at http://www.morningstarpublishing.com/article s/2011/07/27/leader_and_kalka skian/news/doc4e3057 d1f2ce0870416494.txt ).
Moy, P. B., Polls, I., & Dettmers, J. M. 2011. The Ch icago Sanitary and Ship Canal Aquatic Nuisance Species Dispersal Barrier. American Fisheries Society Symposium 74:121-137.
Oregon Sea Grant. Website accessed August 2011. http://seagrant.oregonstate.edu/themes/invasive s/toolkit/Asian-Carp.pdf

. Papoulias, D. M., Chapman, D. C., & Tillitt, D. E. 2006. Reproductive condition and occurrence of intrasex in bighead carp and silver carp in th e Missouri River. H ydrobiologia 571:355-360.

Sass, G. G., Cook, T. R., Irons, K. S., McClelland, M. A., Michaels, N. N., O’Hara, T. M., & Stroub, M. R. 2010. A mark-recapture population estimat e for invasive silver carp ( Hypophthalmichthys molitrix ) in the La Grange Reach, Illinois River. Biol. Invasions 12:433-436.
Sparks, R. E., Barkley, T. L., Creque, S. M., Dettme rs, J. M., & Stainbrook, K. M.. 2011. Evaluation of an Electric Fish Dispersal Barrier in the Chicago Sanitary and Ship Ca nal. American Fisheries Society Symposium 74:139-161.
U.S. Army Corps of Engineers (U SACE) website accessed September 2011. http://www.lrc.usace.ar m y.mil/AsianCarp/eDNA.htm
U.S. Geological Society (USG S). Website accessed August 27, 2011. http://waterdata.usgs.gov/u sa/nwis/uv? site_no=05568615

. Yi, B., Liang, Z., Yu, Z., Lin, R., & He, M. 1988. A St udy of the Early Development of Grass Carp, Black Carp, Silver Carp, and Bighead Carp in the Yangtze River, China. Chapter 2 of Early Development of Four Cyprinds Native to the Yangtze River, Ch ina. U.S. Geological Survey Data Series 239-2, 51 pp. Translated from Chinese to English. Original doc ument published in Yi, B ., Yu, Z., and Laing Z. eds., 1988. Gezhouba water control pr oject and four famous fishes of the Yangtze River, China: Wuhan, China, Hubei Science and Technology Press, pp. 69-135.



### 12.2 References for “Water Transport During Normal operations of Tow boats and Barges in the Illinois River” Bettoli, P. W., W. H. Neill and S. W. Kelsch. 1985. Temperature preference and he at resistance of grass
carp, Ctenopharyngodon idella (Valenciennes), bighead carp, Hypophthalmichthys nobilis (Gray), and their FI hybrid. Journal of Fish Biology 27:239-247.

Chilton III, E. W. and M. I. Muoneke. 1992. Biology and management of grass carp ( Ctenopharyngodon idella , Cyprinidae) for vegetation control: a North Am erican perspective. Review Fish Biology and Fisheries 2:283-320.
Chunsheng, Z., L. Zhixin, and H. Henian. 1980. Ecological features of the spawning of certain fishes in the Hanjiang River after the construction of dams. Acta Hydrobio. Sin. 7(2):188-193.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

45 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Cudmore, B. and N.E. Mandrak. 2004. Biological Synopsis of Grass Carp ( Ctenopharyngodon idella ). Canadian Manuscript Report of Fisheries and Aqua tic Sciences 2705. Fisher ies and Oceans Canada, Great Lakes Laboratory for Fish eries and Aquatic Sciences, Bu rlington ON L7R 4A6, Canada.
FAO. 1980. Freshwater Aquaculture Development in China. FAO Fisheries Technical Paper 215.

Federenko, A. Y. and F. J. Fraser. 1978. Revi ew of grass carp biology. Interagency Committee on Transplants and Introductions of Fish and Aqua tic Invertebrates in British Columbia. British Columbia, Department of Fisher ies and Environment, Fisheries and Marine Service, Technical Report No. 786. 15 pp.
Garcia, J., Reporter for ABC in Chica go, 2009. Story posted on their website

Golovanov, V. K. and A. K. Smirnov. 2007. Influence of the water heating rate upon thermal tolerance in common carp ( Cyprinus carpio L. ) during different seasons . Journal of Ichthyology Volume 47 (7): 538 - 543.
Kolar, C. S., D. C. Chapman, W. R. Courtenay, C. M. Housel, J. D. Williams, and D. P. Jennings. 2007. Bigheaded carps: a biological s ynopsis and environmental risk assessment. American Fisheries Society, Special Publication 33, Bethesda, MD.
Leventer, H. 1979. Biological control of reservoir fish. Mekoroth Water Co., Jordan District Central Lab. Nazareth, Israel.
Negonovskaya, I. T. 1980. On the results and prosp ects of the introduction of phytophagous fishes into waters of the USSR. Journal of Ichthyology 20(4):101-111.
Opuszynski, K., A. Liriski, L. Myszkowski, and J. Wolnicki. 1989. Uppe r lethal and rearing temperatures for juvenile common carp, Cyprinus carpio L ., and silver carp, Hypophthalmichthys molitrix (Valenciennes). Aquaculture Re search Volume 20 (3): 287 - 294.
Shireman, J. V. and C. R. Smith. 1983. Synopsis of biological data on the grass carp, Ctenopharyngodon idella (Cuvier and Valenciennes, 1844). Food and Aquaculture Organization Synopsis. 135: 86 pp.
Tripathi, S.D. 1989. Hypophthalmichthys molitrix (Val.) and Ctenopharyngodon idella (Val.) – Exotic elements in freshwater carp polyc ulture in India. Pages 27-33, In : J. M. Mohan, editor. Exotic aquatic species in India. Special Publica tion 1, Asian Fisheries So ciety, Indian Branch.
Versar, Inc. 1999. Risk assessment of the potential e ffects of stocking triploid-certified grass carp in the Potomac River watershed on submersed aquatic vegetation. Report prepared for U.S. Environmental Protection Agency, Chesapeake Bay Program. Columbia, Maryland.
Wrigley, T.J., Toerien, D.F., Gaigher, I. G., 1988. Fi sh production in small oxidation ponds. Water Res. 22 (10), 1279–1285.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX A TEST PLAN

The study plan was developed for us e during entrainment and survival experiments to be conducted with Asian carp on the Illinois River in June 2011. As noted in the report, high river stag e and lack of Asian carp eggs resulted in modificati ons to the study plan.

The full study plan is available in Volume II, Appendi x A, of this report. Di stribution of the plan was limited to U.S. Coast Guard and their c ontractors prior to the experiments.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX B ASIAN CARP FIELD AND LA BO RATORY DATA SHEETS

The data sheets from the survivability experiments conducted in 2011 are availabl e in Volume II, Appendix B, of this report. They are arranged as follows.

Appendix B1: Times for Fill and Empty of each Tank during Entrainment and Survival Testing Appendix B2: Water Quality Measurement Datasheets Appendix B3: Trial 1. Asian carp entrainment/leakage (Task 3.4) laboratory datasheet Appendix B4: Trial 1. Asian carp survival (Task 3.5) laboratory data sheets – in tank cages Appendix B5: Trial 1. Asian carp survival (Task 3.5) laboratory da tasheets – control cages Appendix B6: Trial 2. Asian carp entrainment/leakage (Task 3.4) laboratory datasheets Appendix B7: Trial 2. Asian carp survival (Task 3.5) laboratory data sheets – in tank cages Appendix B8: Trial 2. Asian carp survival (Task 3.5) laboratory da tasheets – control cages Appendix B9: Trial 2. Plankton Tow Datash eets during Tank Filling

NOTE : The word “Survey” in the datasheets is referred to as “Trial” in the report text. Two (2) trials were conducted for the entrainment, surviv al, and pump effects experiments.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX C SURVEY PHOTOS, AUGUST 2010

Figure C-1. Loaded (upper) and un loaded (lower) barges. Note mi nimal clearance under bridge.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

C-2 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure C-2. Interior of em pty hopper barge (upper). Lo ading hopper barge (lower).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

C-3 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure C-3. Raised and flush hatc h covers on rafted barges (Uppe r). Raised access hatch (lower).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

C-4 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure C-4. Measuring temperature and dissolved oxygen on rafted barge (upper). Interior of barge void space (lower). Water depth approximately 3 inches.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX D SURVEY PHOTOS, JULY-AUGUST 2012

Figure D-1. Hopper barge dry-docked for repair, note hull depth to freeb oard (upper). Hopper barge fully loaded with constructio n grade sand (lower).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

D-2 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure D-2. Loaded barges rafted at construction materi als site (upper). Covers being installed to protect cargo on hopper barge; note added height (lower).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

D-3 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure D-3. Barge being loaded with loose material near dispersal barrier (uppe r). Empty hopper barge at Midwest Generation on CSSC (lower).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

D-4 UNCLAS//Public | CG-926 RDC | D. Heilprin et al Public January 2013 Figure D-4. Recording data aboard towboat in CSSC (upper). Warning si gn on CSSC near dispersal barrier (lower).Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX E 2010 WATER TRANSPORT FI ELD DATA SHEETS

The data sheets from the 2010 survey of barge ballast ta nks are available in Volume II, Appendix C, of this report. Sheets include barge number, location, and am ount of water in tanks. Temperature and dissolved oxygen content are provided for tanks with more than 2-4 inches of water.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX F 2012 WATER TRANSPORT FI ELD DATA SHEETS

The data sheets from the 2012 survey of barge ballast ta nks are available in Volume II, Appendix D, of this report. Sheets include barge number, location, and am ount of water in tanks. Temperature and dissolved oxygen content are provided for tanks with more than 2-4 inches of water.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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### APPENDIX G TOWBOAT AND BARGE DE SIGN AND OPERATION

Towboats: Towboats have void tanks, normally located at the bow or stern of the vessel, wh ich are used for ballasting operations. Ballast water is pumped into a void ta nk (not the bilge area) by an onboard pump through permanent piping. The discharge of this ballas t water is done usually through an onboard pump and permanent piping. Some towboats, however, are not equipped with onboard pumps and therefore use portable pumps. Typical portable pumps are 2-inch or 3-inch trash pumps that re quire suction and discharge hoses.

Towboats may take on ballast water as fuel is consumed and before refueli ng takes place. This ballasting is necessary to keep the towboat trimme d to the operating draft required or de sired for that particular vessel. The ballast water is discharged immediately before re fueling or during refueling. Occasionally ballast may be taken onboard to improve handling and remain in the tanks for months be fore being discharged.

Barges: There are several types of barges: dry cargo hopp er barges, tank barges and deck barges.

Dry cargo hopper barges are the most common barges. They measure 2 00 feet long by 35 feet wide by 12 to 14 feet tall. These barges look like giant empty shoe boxes with the cargo down in the box. These barges are built such that there is one bow tank, 4 or 5 wing (or side) tanks a nd one stern tank. The wing tanks go from one side of the barge, under th e cargo hopper, and to the other side of the barge. At the side of the cargo hopper, the wing tanks are about 3 feet wide. Under the cargo hopper, the tanks are about 18 inches deep. By design, water is free to flow from side to side because there is no cente rline bulkhead (or wall) to prevent this flow of water.

Tank barges have more varied sizes. A 10,000 ba rrel capacity barge measures 200 feet by 35 feet by 12 feet. Larger capacity barges measure 296 feet long by 54 feet wide by 12 feet. The tank barges have cargo boxes like the dry cargo barges a lthough they are cargo tanks. There are usually 3 cargo tanks or compartments per barge. Similar to dry cargo barges, tank barges have a bow tank, 4 or 5 wing tanks and a stern tank. Some tank barges may have a center bulkh ead resulting in separate port and starboard tanks.

Deck barges are constructed to ca rry the cargo on the deck of the barg e and not down in a cargo box. There are few standard sizes for deck barges and they come in many sizes. Deck barges usually have one, if not more, centerline bulkheads. This means that the water cannot flow from one side of the barge to the other side. Deck barges usually have more individual voi d tanks because of the centerline bulkhead(s). The centerline bulkhead may or may not dissect the bow and st ern tanks into two or more separate tanks. Unlike dry cargo barges, deck barges do not have wing tanks but rather void tanks that ar e much larger than wing tanks. These void tanks can be 12 – 14 feet deep sin ce they reach from the ba rge hull to the deck.

Barges do not have permanent piping to pump water in to or out of their tanks, portable pumps are used. Barges may be ballasted to clear lo w bridges such as the low railroad bridge in Lamont. Barge tanks are inspected regularly. Operators quest ioned about standard ba llasting practices for th is study indicated that tanks are inspected once per 6-hour watch when ac tive or once per day if rafted and inactive.Asian Carp Survivability Experiments and Water Transport Surveys in the Illinois River, Volume I

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