**-  South Atlantic  
-  Raleigh, NC 
-  Plant Science Research 
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**Location: Plant Science Research**
**2009 Annual Report**
**1a.Objectives (from AD-416)**													Characterize perchlorate accumulation in plants in response to ozone exposure, and determine whether the response is different for ozone-sensitive and tolerant plants.						
**1b.Approach  (from AD-416)**													Grow ozone-sensitive and tolerant snap bean lines (developed by parent project) under field conditions and analyze leaf tissue for total perchlorate content and the distribution of natural abundance oxygen-16 and oxygen-18 in perchlorate purified from these leaves.						
**3.Progress Report**													This project is related to inhouse objective 1:  Quantify the combined effects of elevated carbon dioxide and ozone concentrations on soil carbon dynamics in a soybean-wheat field crop system; quantify the effects of temperature on the physiology, biomass production and seed yield of selected crop plants under conditions of elevated carbon dioxide and ozone; and identify soybean germplasm with enhanced growth and yield potential under elevated levels of carbon dioxide and ozone.
The objective of this project is to identify and characterize natural sources of perchlorate in plants. Ozone-sensitive and tolerant plants are used to assess potential contribution of ambient ozone to perchlorate accumulation in leaf tissue.
Plans were developed and implemented to conduct a field experiment at two locations for production of large quantities of snap bean leaf tissue to be used by Department of Defense collaborators for perchlorate extraction and analysis.
Pre-season preparations included:.1)winter greenhouse seed increases for the ozone-sensitive S156 and ozone-tolerant R123 snap bean genotypes,.2)identification of a low perchlorate fertilizer to provide plant nutrition during the growing season, and.3)testing irrigation water at the two locations for perchlorate content and development of an in-line filter to reduce perchlorate in irrigation water in order to minimize irrigation as a source of perchlorate accumulation in the plant tissues.
During the summer months three replicated field plots for each genotype containing 1000-1500 plants per plot were established at two locations for production of leaf tissue.  Locations were:.1)Raleigh, NC in a field maintained by parent project staff, and.2)the Long Island Horticultural Research & Extension Center, Riverhead, NY by staff of a cooperator supported through a Specific Cooperative Agreement with Cornell University.  Leaf tissue was harvested and dried according to established protocols, and then shipped to Department of Defense collaborators at Texas Tech for perchlorate extraction and analysis.  Samples of soil, irrigation water, and rain were collected for testing as potential sources of perchlorate in the resulting plant tissue.  Environmental data including ambient ozone concentrations, air temperature, relative humidity, light levels, and rainfall were continuously recorded at each location.						
    Project TeamBurkey, Kent  
Project Annual Reports FY 2009 
Related National Programs Air Quality(203) Global Change(204)  							Last Modified: 04/14/2010 		ARS Home |USDA.gov |Site Map |Policies and Links FOIA |Accessibility Statement |Privacy Policy |Nondiscrimination Statement |Information Quality |USA.gov |White House

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