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SUBMITTING ENCLOSED INFORMATION Q~ ALICRRB SOLUTIONS IN

DI CHL.l]fi OJ1E;H;il·:E AE;~:030L H~HALAHO~I (lCUTE TOXIC ITY STUD Irs

W-ATTACH

ALDICARB SOLUTIONS IN DICHLOROMETHANE
B{e)

(COMPLIANCE AUDIT PROGRAM)

TSCA CONFIDENTIAL

BUS\NESS INFORMAT'ON

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RETURN RECEIPT REQUESTED

38 OLD FlIOGEBURY R~Ic:L)(N~(iRYAftff!3lj1r-0001

JFIIQ- O,fJC;;l-7C)t:7;C

August 21, 1992

UNION CARBIDE CORPORATION

Document Processing Center (TS-790)

Room L-100

Office of Toxic Substances

U.S. Environmental Protection Agency

401 M Street, SW

Washm.gton, DC 20460

8892888&&38

Attn: Section 8(e) Coordinator (CAP Agreement)

Re: CAP Agreement Identification No. 8ECAP-Oll0

Dear Sir or Madam:

Union Carbide Corporation ("Union Carbide") herewith submits the following reports

pursuant to the terms of the TSCA §8(e) Compliance Audit Program and Union Carbide's CAP

Agreement dated August 14, 1991 (8ECAP-Oll0). These reports describe aerosol inhalation and

acute toxicity studies with Aldicarb solutions in dichloromethane (DCM). The CASRN for

Aldicarb (TEMIK®) is 116-06-3.

(1) "Aldicarb Solution (in DCM) LCso Aerosol Acute Inhalation Toxicity Test", Bushy Run

Research Center, Project Report 48-136, December 11, 1985.

(2) "Aldicarb in DCM (3 Solutions): Acute Peroral Toxicity Study - D.O.T. Classification",

Bushy Run Research Center, Project Report 49-65 Revised, May 15, 1986.

Complet~ ~"Ummaries of these reports are attached.

Previous TSCA Section See) or "FYI" Submission(s) related to this substance are:

(None)

Previous PMN submissions related to this substance are: (None)

49-65
This information is submitted in light of EPA's current guidance. Union Carbide does

not necessarily agree that this information reasonably supports the conclusion that the subject

chemical presents a substantial risk of injury to health or the environment.

In the attached reports the term "CONFIDENTIAL" may appear. This precautionary

statement was for internal use at the time of issuance of these reports. Confidentiality is hereby

waived for purposes of the needs of the Agency in assessing health and safety information. The

Agency is advised, however, that the publication rights to the contained information are the

property of Union Carbide.

Yours truly,

W~D. Associate Director

Product Safety

(203/794-5230)

WCKlcr

Attachment (3 copies of cover letter, summaries, and repor.is)

49-65
SmwARy

BUSHY RUN RESEARCH CENTER

R. D.•, Mellon Roall, Export, Pennsylvania 15632 Telephone 1412) 733·5200

Project ~eport 48-136

Aldicarb Solution (in DCH)

le50 Aerosol Acute Inhalation Toxicity Test

Sponsor: Union Carbide Agrucultural Products Company

* * * * *

ABSTRACT

Five groups of five male and five female Sprague-Dawley albino rats were

exposed once for four hours to an aerosol of aldicarb solution in

dlchloromethane (DeM). The concentration of the individual components of the

test material (aldicarb, dichloromethane and methyl isocyanate) in the chamber

atmosphere were determined by chromatographic methods. The mean aldicarb

concentrations for the five exposures were 0.0463, 0.0087, 0.0060, 0.0020, and

0.00082 mg/l. The mean dlchloromethane (DCM) concentrations for the same

exposures were 0.166, 0.J25, 0.183, 0.094 and 0.043 mg/l. No methyl isocyante

was detected in the chamber atmosphere. The mass median aerodynamic diameter

for the aerosol particle size distributions ranged from 2.0 to 3.8 micrometers

and the geometric standard deviations ranged from 2.5 to 5.0. Mortalities

were seen in all but the 0.00082 mg/l male exposure group, and the 0.0020 and

0.00082 mg/L female exposure groups. Ataxia and tremors were seen i 11

concentration groups during exposure. Addit ona c n cal signs observed in

some groups on the day of exposure or during the postexposure period 'included

periocular, perinasal, and perioral wetness and encrustation, urogenital

wetness, lacrimation, exophthalmos, discolored and unkempt fur, an increased

or decreased respiration rate, abdominal breathing, d~creased motor activity,

and a"slow righting reflex. No clinical signs were observed in survivors

during the second week of the postexposure period. A loss of body weight or

depressed body weight gains were observed in some animals on postexposure

day 7. However, body weight gains were observed for all survivors on postexposure day 14. A discoloration of the lungs, perinasal encrustation and

staining, and eye abnormalities were observed at necropsy in some animals

which died. The lC50 (with 95% confidence limits) for the combined sexes was

0.0039 (0.0028 to 0.0055) mg/L aldlcarb.
SUltWARY

BUSHY RUN RESEARCH CENTER

R. D. 4, Mellon Roed, Export, Pennilivanle 11832 Telephone (4121 733-5200

Project Report 49-65 Revised

Aldiearb in PCM (3 Solutions>

Acute Peroral Toxicity Study - P.O,T. Classification

Sponsor: Union Carbide Agricultural Products Company

SUMMARY

A rat peroral toxicity test was completed on each of 3 concentrations of

aldicarb in DCM. The procedure followed the standard BRRC protocol modified to

conform to Department of Transportation (D.O,T.) test conditions. Results from

this study, expressed in terms of each solution as received, are as follows:

Peroral, Rat (Fasted)

1,0% Concentration

Males: 50 mg of solution per kg of body weight (b,w.)

killed 5 of S.

Females: 50 mg per kg b,w, killed 5 of 5.

Combined: 50 mg per kg b.w. killed 10 of 10.

- 0,5% Concentration

Males: 50 mg per kg b.w. killed one of 5.

Females: 50 mg per kg b.w. killed 3 of S.

Combined: mt..per kg b.w. killed 4 of 10.

0,3% Concentration

Males: 50 mg per kg b.w, killed 0 of 5.

Females: 50 mg per kg b.w. killed 0 of 5.

Combined: ~er kg b.w. killed 0 of 10.

RESULTS

.-...-. -~.:... .

Results from the peroral study are given in Tables 1, 2 and 3 for the

1.0%, 0.5% and 0.3%concentratlons, respectively. All 5 male and 5 female

rats died following peroral doses of 50 mg/kgb.w.of the 1.0% aldicarb in

DCM. Signs of toxicity included sluggishness, tremors, salivation,

lacrlmation.and red tearing. Deaths· occurred at 15 minutes.to 1.5 hours.

necropSY, lungs wer.e dark red and periocular fur' h__d a red crust•.
R. O. ". Mellon ROlRd, Export. ~.nnlyIY.nl. 15632

BUSHY RUN RESEARCH CENTER

Telephone 14121 733·5200

vee BUSINESS CONFIDENTIAL: Not to be released

outside vec without the written consent of

Dr. R. L. Baron, Manager of Toxicology,

Agricultural Products Company.

Project Report 48-136

30 Pages

December II, 1985

Aldicarb Solution (in DCM)

Le50 Aerosol Acute Inhalation Toxicity Test

Sponsor: Union Carbide Agrucultural Products Company

* * * * *

ABSTRACT

F~ve groups of five male and five female Sprague-Dawley albino rats were

expcsed once for four hours to an aerosol of aldicarb solution in

di~hloromethane (DeM). The concentration of the individual components of the'

test material (aldicarb, dichloromethane and methyl isocyanate) in the chamber

atmosphere were determined by chromatographic methods. The mean aldicarb

concentrations for the five exposures were 0.0463, 0.0087, 0.0060, 0.0020, and

0.00082 mg/L. The mean d1chloromethane (DCM) concentrations for the same

exposures were 0.166, 0.025, 0.183, 0.094 and 0.043 mg/L. No methyl isocyante

was detected in the chamber atmosphere. The mass median aerodynamic diameter

for the aerosol particle size distributions ranged from 2.0 to 3.8 micrometers

and the geometric standard ,deviations ranged from 2.5 to 5.0. Mortalities

were seen in all but the 0.00082 mg/L male exposure group, and the 0.0020 and

0.00082 mg/L female exposure groups. Ataxia and tremors were seen 11

concentration groups during exposure. Addit ona c n cal signs observed in

S'Ome groups on the day of exposure or during the postexposure period included

periocular, perinasa1, and perioral wetness and encrustation, urogenital

wetness, lacrimation, exophthalmos,' discolored and unkempt fur, an increased

or decreased respiration rate, abdominal breathing, decreased motor activity,

and a slow righting reflex. No clinical signs were observed in ,survivors

during the second week of the postexposure period. A loss of body weight or

depressed body weight gains were observed in some animals on ppstexposure

day 7. However, body weight gains were observed for all survivors on postexposure day 14. A discoloration of the lungs, perinasal encrustation anc

staining, and eye abnormalities were observed at necropsy in some animals

which died. The LC50 (with 95% confidence limits) for the combined sexes was

0.0039 (0.0028 to 0.0055) mg/L aldicarb.

BustiyRun ~esea,ch,Center "" ' '

A Joint MeUon Instltute-Union Carbide Corporatio,n Op,eration, - .. .'.' . ',: '.', '.:'.,. '" '.~_.:.-:': _,.::..".. ,' '...;: .,;-,~ '~,. .:<..'.:: ...:.::> ,:':;",:,,i,'.::\ ',:~i:~;~l;:'" '.::'~;:":': .;::;~,:'-: ::"
Report 48-136

Page 2

OBJECTIVE

This study was designed to determine the acute inhalation Le50 to rats

resulting from a four-hour exposure to aldicarb solution (in DCM)

MATERIALS AND METHODS

This study followed the specific protocol (BRRC Project 85-03-40150) and

standard amendment prepared by the Bushy Run Research Center.

Test Article

Two, one-quart bottles of aldicarb solution (in DCM) (CAS Registry Number

116-06-03; Reference Number HTS 5819AA) were received on May 23, 1985, from

Union Carbide Agricultural Products Company, Research Triangle Park, NC,

assigned BRRC Sample Number 48-121 A & B, and stored in Room 137. No chemical

analyses were performed on the purity or composition of the material.

Information received from the Sponsor stated the test material to be a

formulation containing 37% aldicarb, 59% dich1oromethane, and a maximum of

5000 ppm methyl isocyanate (MIC). The Sponsor indicated the test material to

be very stable at 20°C.

Animal Species, Source and Husbandry

Male and female ZM/Sprague-Daw1ey albino rats (Zivic-Mi11er Laboratories,

Inc., Allison Park, PA) were used. The age of the male and female rats on the

day of exposure is listed in the following table:

Alicarb

Expo·sure Date Age at

Concentration (mg/L) Sex Received Exposure (days)

0.0463 M July 2, 1985 52

0.0463 F July 2, 1985 56

0.0087 M July 23, 1985 41

0.0087 F July 16, 1985 54

0.0060 M July 23, 1985 47

0.0060 F July 16, 1985 60

0.0020 M July 23, 1985 46

0.0020 F July 16, 1985 59

0.00082 M July 16, 1985 56

0.00082 F July 16, 1985 58
Report 48-136

Page 3

The rats were assigned unique identification numbers by toe-clipping and

were housed two or three per cage in 23.5 x 20 x 18 em high stainless steel

wire mesh cages on carriers in Rooms 109 and l64E for the quarantine and

postexposure periods. respectively. The rats were kept on a l2-hour

photoperiod throughout the entire study period. A layer of Deotized Animal

Cage Board- (Shepherd Specialty Papers, Inc., Kalamazoo. MI) was placed under

each row.of cages. Pelletal feed (Certified Rodent Chow 5002. Ralston Purina

Co •• St. Louis, MO) and tap water (Mun~cipal Authority of Westmoreland County,

Greensburg. PA) were available ad libitum except during exposure.

Inhalation Chamber Design and Operational Characteristics

The rats were individually housed in 21 x 12.5 x 18 cm wire mesh cages and

exposed in a l330-1iter (approximate volume) rectangular with pyramidal top and

bottom stainless steel and glass chamber in Room 137. The inhalation chamber

design and operation are summarized in Table 1.

Exposure Regimen

Five groups of five male and five female rats were exposed once for four

hours to an aerosol atmosphere of aldicarb solution (in DCM). No control

exposures were performed.

Generation of the Test Material

The aldicarb solution (in DCH) aerosol was generated using a Spraying

Systems No. lA syphon spray set-up, (No. 1650 liquid nozzle and No. 64 air

nozzle). The atomizer was inserted into the top of the inhalation chamber

turret where the liquid aerosol was diluted to the desired concentration and

dispersed throughout the chamber by filtered air. A laboratory pump was used

to deliver the test solution to the atomizer. The test material used in the

0.0463 and 0.0087 mg/L exposures was undiluted. The extremely low flowrates of

test material reqUired for the lowest three concentrations of aldicarb

necessitated that the test material be diluted ten-fold (w/w) with DeM.

To prevent clogging of the atomizer, an air piston (Bimba Model 091-NR)

controlled by an electric valve (Skinner Model Bi4DKl075) and interfaced with a

digital valve sequence programmer (Model DVSP-3) was used to actuate the

clean-out needle of the atomizer at 10 second intervals. FiguLe 1 depicts a

schematic diagram of the generation and exposure system. The operating

conditions and the resulting chamber air flowrates are presented in the

following table:
Report 48-136

Page 4

Total

Aldicarb Atomizer Chamber

Exposure Air Air

Concentration Atomizer Pressure Flowrate Pump*

(mg/L) Type (psig) (L!min) Type and Setting

0.0463 Spraying Systems 15 300 FMI RP-G-6; 1/8"

piston; set at 200

0.0087 Spraying Systems 7 560 Sage pump with 30 ml

syringe; set at 1/100

and 5.5

0.0060 Spraying Systems 7 560 Sage pump with 30 ml

syringe; set at 1/100

and at 15.0 or 16.8

0.0020 Spraying Systems 7 560 Sage pump with 30 ml

syringe; set at 1/100

and 8.4

0.00082 Spraying Systems 7 560 Sage pump with 30 ml

syringe; set at 1/100

and 4.2

*FMI Lab Pump (FMI Fluid Metering) Inc., Oyster Bay, NY)

Sage Model 355 Syringe Pump (Sage Instruments, Division of Orion Research,

Cambridge MA)

I \ Target Concentration

The target concentration for the first exposure was 0.2 mglL of aldicarb

solution (in DCM) equivalent to approximately 0.05 mglL aldicarb. This

concentration was selected based on the results of theD.O.T. aerosol test

performed at BRRC on July 10, 1985 (BRRe Report 48-134). The target

concentration for subsequent groups was based on the observed mortality of

previous groups.

Chamber Concentration Analysis

Since the DCM concentrations posed an 1nsignficant acute toxicity hazard in

comparison to aldicarb, the concentrations presented for this report and the

LCSO calculations were based on the aldicarb concentrations, and not the

a1dicarb solution (in DCM) concentrations.

The concentration of aldicarb, dichloromethane t and methyl isocyanate in

the exposure chamber was determined during the five exposures by , .

chromatographic methods. The total test materIal concEmtrat1on of~ldicarb

solution (in DCM) in the exposure chamber atmosphere was calculated by adding

the individual component ccincentrations.A.complete desCrip.t1"Ilof theeL'"

analytical methods used is given .inthe Analyt~alCliemisdyReport, (Appendix 1): .. ;' '; c'. -,'-.;:3:'<
Report 48-136

Page 5

The nominal concentration of the aldicarb solution (in DCM) was determined

by dividing the total weight of the solution generated by the volume of air

which passed through the chamber during the exposure period. The value for

total weight was estimated by multiplying the number of milliliters used by

the specific gravity (1.23 g/ml) of the test material.

Particle Size Distribution

The particle size distribution of the aldicarb solution (in DCM) aerosol

was not obtained for the 0.0463 mg/L exposure due to the short exposure time.

Only one particle size sample was obtained for all other groups, generally due

to the long sample collection times. A Sierra 8-stage cascade impactor

(Series 210, Sierra IU6tru~ents, Inc., Carmel Valley, CA) was usee to obtain

the chamber samples of the aerosol. Sierra C-220 WH filters were used to

collect the aerosol on the stages and the filters were assayed

gravimetrically. The mass m~dian aerodynamic diameter and geometric standard

deviation were determined from log probability plots of the cumulative percent

mass collected on each impactor 9tage.

Temperature and Relative Humidity

The temperature and relative humidity in the animal housing rooillwere

recorded continuously with a seven-day recording hygrothermograph (Cole-Parmer

Instruments, Chicago, IL). During the exposure the temperature was monitored

with a minimum-maximum thermometer (Abbeon Cal. Inc., Santa Barbara, CA) and

the relative humidity was monitored with an'Airguide Humidity Indicator

(Airguide Instrument Company, Chicago, IL).

Animal Observation

All animals were observed on the day of exposure, and for 14 days

following exposure for signs of toxic effects.

Body Weights

The animals were weighed prior to exposure and on post-exposure days seven

and fourteen. The change in body weight was calculated by subtracting the

pre-exposure values from each successive weight.

Necropsy

. The animals were sacrificed following methoxyflurane anesthesia. Th~. rats

were exsanguinated by severing tile brachial blood vessels and a complefe"" . ,

necropsy was performed. The lungs, liver, kidneys, nasal passages, and any

a~normal tissues were placed in a fixative and saved for,p-0Sl.sible,fuf:urec

histologic examination. The following table relates the exposure and terminal ~acrlf1ce dates: :.~
Repcrt 48-136

Page 6

Date of

Ter'llinal

Sacrifice

•

* August 14, 1985

August 13, 1985

August 12, 1985

Exposure Date

july 12, 1985"

July 25, 1985

July 31, 1985

July 30, 1985

July 29, 1985

~~dicarb Exposure

Concentration

(maIL)

*No terminal sacrific~-al1 animals died during exposure or 14-day postexposure

observation period.

Statistical Procedures

The mean and standard deviations of the body weights, body weight changes,

and exposure concentrations were calculated. No statistical comparisons were

made. The LC50 was determined by a modified met~od of Finney's probit analysis

(1952) •

Storage of Records

The final report, tissue specimens, and all raw data are retained in the

Archives of the Bushy Run Research Center.

RESULTS

Chamber Concentration

Analytical results are presented in the Analytical Chemistry Report

(Appendix 1). The mean aldicarb concentration for the five exposures were

0.0463, 0.0087, 0.0060, 0.0020 and 0.00082 mg/L. The mean dichloromethane

(DCM) concentrations for the same exposures were 0.166, 0.025, and 0.183, 0.094

and 0.043 mg/L. No methyl isocyanate was detected in the chamber atmosphere.

The mean measured concentration of aldicarb solution (in DCM), calculated by

addition of the individual components, and the nominal concentrations for the

five exposures are presented in Table 2.

Particle Size Distribution

The resu1t·s of the impactor samples are presented in the follOWing table:

Aldicarb Exposure Sample Sample Mass Median

Concentration Flowrate Duration Aerodynamic

(mg/l) (Llmin) (min) Diameter (mm)

0.0463 --*-- * * 0.0087 8.8 45 3.4

0.0060 8.8 90 3.6

0.0020 8.8 180 3.8

0.00082 8.8 180 2.0

GeometriC

Standard

Deviation

*

4.1

2.5

3.9

5.0

*The particle size distribution of the aldicarb solution (in DeM) aerosol was

not determined for this exposure level. All animals died before a '.' sample of ':"i.;.:;

the aerosol could be obtained.

Figures'2 through 5 present a graphical presentation of these
Report 48-136

Page 7

Humidity and Exposure Chamber Conditions

The temperature and relative humidity in the animal housing room ranged

from 6S to 72°F and 46 to 69%, respectively, throughout the study period. The

temperature and humidity data for the exposure chamber are presented in Table 1.

Animal Observations

Animal observations during the exposure and postexposure periods are

summarized in Tables 3 (male rats) and 4 (female rats). Clinical signs of

toxicity were observed in all exposure groups. Clinical signs observed during

exposure for the 0.0463 and 0.0087 mg/L exposure groups included ataxia,

tremors. hypoactivity. an increased respiration rate, lacrimation,

exophthalmos, and the presence of a red periocular encrustation. The only

clinical signs of toxicity observed during exposure for the 0.0060, 0.0020. and

0.00082 mg/L exposure groups were ataxia and tremors. Addition signs observed

following exposure or during the postexposure period for the three lower

exposure concentration groups included periocular. perinasal. and perioral

wetness, discolored and unkempt fur, decreased motor activity, a slow mid-air

righting reflex, abdominal breathing. and a decreased respiration rate. No

clinical signs of toxicity were observed in survivors during the second week of

the postexposure period.

Mortality

The folloWing table presents a summary of the mortality observed during the

study:

Aldicarb Exposure Time of Death

Concentration During Total

(mg/L) Sex Exposure Postexposure Day 1 Incidence

0.0463 M 5 0 515

0.0463 F 5 0 5/5

0.0087 M 5 0 515

0.0087 F 5 0 515

0.0060 M 0 2 2/5

0.0060 F 1 2 3/5

0.0020 M 0 I lIS

0.0020 F 0 0 0/5

0.00082 M 0 0 O/S

0.00082 F 0 0 0/5

The LC~~ (with 95% confidence limits) for males was determined to be .

0.0038 (0.0021 to 0.0071) mg/L; for females 0.0044 {O.0029toO.0069)mg/Li

and for the combined sexes it was 0.0039 (0.002atoO.00S5) mg/L.'Xhe slopes

for the dose-mortality curves for male rats, female rats,and thecombiried .

sexes were .2.22, 3.86 and 3.04, .,respectively. . , 'i
Report 48-136

·Page 8

Body Weights

The individual body weights and body weight changes are summarized in

Tables 3 (male rats) and 4 (female rats). A loss of body weight was observed

in some rats on postexposure day 7 in the 0.002 mg/L male, and 0.006 and 0.002

mg/L female exposure groups. DeprEssed body weight gains were observed for

two female rats from the 0.00082 mg/L exposure group on postexposure day 7.

Body weight gains were observed for all survivors on postexposure day 14.

Necropsy

Necropsy findings are summarized in Tables 5 and 6. Individual animal

findings are provided in the pathology sheets in Appendix 2, The principal

gross lesions observed in animals vhich died included a disc.,loration of the

lungs, perinasal encrustation and staining, and eye abnormalities (periocular

encrustation. periocular staining, lenticular opacity and corneal opacity).

No significant dose-related macroscopic lesions vere observed in rats

sacrified at the end of the l4-day observation period. The enlarged cervical

lymph nodes observed in some of the survivors and mortalities are a frequent

finding in this strain and are not considered to be exposure related.

DISCUSSION

The aldicarb chamber concentrations for the five aerosol exposures were

0.0463. 0.0087. 0.0060, 0.0020, and 0.00082 mg/L. The dichloromethane

C0ncentrations for the same exposures were 0.166, 0.025, 0.183, 0.094 and

0.043 mg/L. No methyl isocyanate was detected in chamber atmospheres.

Complete mortality ~as observed in the 0.0463 and 0.0087 mg/L aldicarb

exposure groups. The only groups with no mortalities were the 0.0020 mg/L

males, and the 0.00082 mg/L exposure group (both sexes). Ataxia and tremors

vere seen in all groups during exposure. In addition, clinical signs of

ocular and nasal irritation, perioral wetness and encrustation. respiratory

difficulties, and ~oordination loss vere observed in some exposure groups on

che day of exposure or during the first week of the postexposure period.

Hovever, no clinical signs vere observed in survivors during the second week

of the postexposure period. Although Bome animals were observed to have a

loss of body weight or depressed body weight gains on postexposure day 7, body

weight gains vere observed for all survivors on postexposure day 14. A

discoloration of the lungs, perinasal encrustation and staining, and eye

abnormalities were observed in some animals which died. The LC50 for aldicarb

(vith 95% confidence limits) for male rats was 0.0038 (0.0021 to 0.0071) mg/L;

for females 0.0044 (0.0029 to 0.0069) mg/L; and for the combined sexes it wa~

0.0039 (0.0028 to 0.0055) mg/t.

Project Tearn

D. J. Nachreiner, B.S.

D. W. Fait, B.S.

M. L. Steel, AALAS, Cert. I

D. R. Klonne, Ph.D.

M. J. Cardella. , AALAS Cert.

P. A. Baker, RN

II

Study Director

Master Technologist

Senior Technolog1s~

ProjectMana~er; . Animal' Care.Supervisor:. ., ....'.

Necropsy SuperVisor. .": ,.'.,
Reviewed and Approved by:

Report 48-136

Page 9

LCl\t<.i)"? J/-,J..'1.L<.~ / I .'..

Donald J. Nachreiner. B.S. Date

Study Director

? ....,......,~i~' !:.·(N t".

Dennis R. Klonne. Ph.D.

Project Manager

---::::'

~/'7<£=-.~ Fred R. Frank. Ph.D.

Director

REFERENCES

1/"/'· ...:-:;

Date

;zft</

Date

1. Finney. D. J. (1952). Probit Analysis, 2nd Ed., Cambridge University

Press, London, England.

WPC/rkk/0909B-4

11-11-85
Report 48-136

Page 10

Table 1

Inhalation Chamber Design and Operation

Aldicarb Solution (in DCM) LeSe Aerosol Acute Inhalation Toxicity Test

CHAMBER

Location: Room 137

Construction: The chamber was made of stainless steel and glass.

Shape: Rectangular with pyramidal top and bottom

Dimensions: Height - 1.32 meter

Length - 0.91 meter

Width - 0.85 meter

Total Volume - Approximately 1330 liters

Airflow Measurement: An orifice plate was positioned in the exhaust duct

of the chamber and was connected to a Dwyer Magnehelic e pressure gauge

(chamber airflow). A critical orifice. calibrated with a Singer e dry

gas test meter. was used for the impactor samples.

Test Article Generation: A Spraying Systems Atomizer was used to generate

aerosol~ of aldicarb solution (in DCM). Undiluted test material was used

for the 0.0463 and 0.0087 mg!L exposures; for all other exposures, the

test material was diluted ten-fold (w!w) with DCM.

Chamber

Number

Chamber Atmosphere Conditions:

Aldicarb

Exposure

Concentration

(mg!L)

Mean ± SD

Temperature*

(OF)

Mean ± SD

Relative

Humidity**

(%)

37-1

37-1

37-1

37-1

37-1

0.0463

0.0087

'0.0060

0.0020

0.00082

75 ± 0

72 ± 1

71 ± 0

70 ± 0

72 ± 1

33 ± 1

34 ± 1

35 ± 0

35 ± 0

36 ± 1

II: Determined using an Abbeon minimum-maximum thermometer.

** Determined using an Airguide Certified Hygrometer.

WPC/rkk/0909B-2

10-03-85
Report 48-136

Page 11

Table 2

Nominal Exposure Concentrations

Aldicarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test

== Aldicarb Sol-* Total Aldicarb 501-111:

Aldicarb ution (in DCM) Weight Chamber ution (in DCM)

Exposure Exposure Exposure of Test Air Nominal

Concentration Concentration Duration Material Flowrate Concentration

(mg/L) (mg/L) (min) Used (grams) (L!min) (mg/L)

0.0463 0.2123 60 9.90 300 0.5500

0.0087 0.0337 240 9.84 560 0.0732

0.0060 0.1890 240 22.14 560 0.1647

0.0020 0.0960 240 15.87 560 0.1181

0.00082 0.0431 240 5.23 560 0.0389

* The aldicarb solution (in DCM) concentration in the exposure chamber

atmosphere was calculated by adding the individual component concentrations of

aldicarb and DCM. No methyl isocyanate was detected in the chamber atmosphere.

** The a1dicarb solution (in DCM) used for the 0.0463 and 0.0087 mg/L exposures

was undiluted; for the remaining 3 concentrations the test material was

diluted ten-fold (w/w) with DeM.

WPC/rkk/0909B-3

11-11-85
Individual Animal

Aldicarb Solution

Table 3

Report 48-136

Page 12

IL Aldicarb Male Rats*)

Inhalation Toxicity Test

Animal

Number Weights (g) at Day 0

85-16768 283

85-16765 290

85-16764 280

85-,16767 272

85-16766 260

Mean: 277

Standard Deviation: 11

Days to Death

During exposure

During exposure

During exposure

During exposure

During exposure

Mortality

Ratio: 5/5

Group Observations: Clinical signs observed during the exposure were ataxia,

tremors, an increased respiration rate, hypoactivity, lacrimation, and a red

periocular discoloration. Mortalities occurred within 60 minutes of exposure.

Exposure Date: July 12, 1985

* Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison

Park, PA, were used.

(Continued)
Report 48-136

Page 13

Table 3

(Continued)

Individual Animal

Aldicarb Solution

/L Aldicarb Male Rats*)

Inhalation Toxicity Test

Animal

Number Weights (8) at Day 0 Days to Death

85-17038 256 During exposure

85-17040 267 During exposure

85-17036 284 'During exposure

85-17039 274 During exposure

85-17037 294 During exposure

Mean: 275 Mortality

Standard Deviation: 15 Ratio: 5/5

Group Observations: Clinical signs observed during the exposure were ataxia,

tremors, an increased respiration rate, hypoactivity, lacrimation, exophthalmos,

and a red periocular discoloration. Mortalities occurred between 25 and 240

minutes of exposure.

Exposure Date: July 25, 1985

* Albino rats (ZM/Sprague-Daw1ey), Zivic Miller Laboratories Inc., Allison Park,

FA, were used.

(Continued)
Report 48-136

Pag@ 14

Table 3

(Continued)

Individual Animal Body Weight Data (0.0060 mg/L A1dicarb Male Rats*)

Aldicarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test

Animal Weights (g) at Day Body Wt. Change

Number 0 7 14 7 14 Days to Death

85-17442 248 280 362 32 114 14 (S)

85-17448 260 276 353 16 93 14 (S)

85-17447 254 1

85-17449 255 283 357 28 102 14 (S)

85-17451 272 1

Mean: 258 280 357 25 103 Mortality

Std. Dev. : 9 4 5 8 11 Ratio: 2/5

Group Observations: Clinical signs observed d~ring exposure were ataxia and

tremors. Additional signs of toxicity observed fo11o~ing exposure were periocular, perinasa1. perioral, and urogenital wetness, and decreased motor

activity. During the postexposure period. signs observed were periocular,

perinasa1, and perioral encrustation, discolored and unkempt fur, decreased

motor activity, a slow mid-air righting reflex, abdominal breathing. and a

decreased respiration rate. No clinical signs Gf toxicity were observed on

postexposure days 5 through 14.

Exposure Date: July 31, 1985

(S) • Sacrificed on August 14, 1985

* Albino rats (ZM/Sprague-Daw1ey), Zivie Miller Laboratories Inc., Allison

Park, PA, were used.

(Continued)
Report 48-136

Page 15

Table 3

(Continued)

Individual Animal Body Weight Data (0.0020 mg/L Aldicarb Male Rats*)

Aldicarb Solution (in DCM) LCSO Aerosol Acute Inhalation Toxicity Test

AnImal !~ights (g) at Day Body Wt. Change

Number 0 7 14 7 14 Daxs to Death

85-17408 250 246 333 -4 83 14 (S)

85-17407 242 1

85-17406 254 285 37? 31 118 14 (S)

85-17410 247 273 357 26 110 14 (S)

85-17409 254 271 356 17 102 14 (5)

Mean: 249 269 355 18 103 Mortality

Std. Dev. : S 16 16 15 15 Ratio: 1/5

Group Observations: Clinical signs observed during exposure were ataxia and

tremors. Additional signs of toxicity observed following exposure were periocular, perinasal, peroral, and urogenital wetness, decreased motor activity,

abdominal respiration, and a decreased respiration rate. During the postexposure period, signs observed included periocular, perinasal, and perioral

encrustation, discolored and unkempt fur, ataxia, decreased motor activity, a

slow mid-air righting reflex, abdominal breathing, and a decreased respiration

rate. No clinical signs of toxicity were observed in one animal by postexposure day 1, in 3 animals by postexposure day 2, and in all survivors on

postexposure days 6 through 14.

Exposure Date: July 30, 1985

(5) - Sacrificed on August 13, 1985 * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison

Park, PA, were used.

(Continued)
Report 48-136

Page If)

Table 3

(Continued)

Individual Animal Body Weight Data (0.00082 mg/L Aldicarb Male Rats*)

Aldlcarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test

Animal Weights (g) at Day Body Wt. Change

Number 7 14 7 14 Days to Death

85-17249 290 307 386 17 96 14 (S)

85-17250 300 337 396 37 96 14 (5)

85-17251 294 334 399 40 105 14 (5)

85-17242 300 338 407 38 107 14 (5)

85-17240 300 329 404 29 104 14 (5)

Mean: 297 329 398 32 102 Mortality

Std. Dev. : 5 13 8 9 5 Ratio: 0/5

Group Observations: Clinical signs observed during exposure were ataxia and

tremors. No clinical signs of toxicity were observed following exposure.

During the postexposure period, clinical signs were observed only on postexposure day I for 3 rats. The clinical signs observed were periocular, perinasal, and perioral encrustation, discolored and unkempt fur, decreased motor

activity, abdominal breathing, and a decreased respiration rate.

Exposure Date: July 29, 1985

(5) = Sacrificed on August 12, 1985

* Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison

Park, PA, were used.

WPC/rkk/0909B-2

10-03-85
Report 48-136

Page 17

table 4

IndiYidual Animal

Aldicarb SolutIon

ht Data (0.0463 m /L Aldicarb Female Rats*)

LC50 Aerosol Acute Inhalation Toxicity Test

Animal

Number Weights (g) at Day 0 Days to Death

85-16782 237 During exposure

85-16785 261 During exposure

85-16784 261 During exposure

85-16786 288 During exposure

85-16783 252 During exposure

Mean: 260 Mortality

Standard Deviation: 19 Ratio: 5/5

Group Observations: Clinical signs observed during the exposure were ataxia,

tremors, an increased respiration rate, hypoact1vity, lacrimation, and a red

periocular discoloration. Mortalities occurred within 60 minutes of exposure.

Exposure Date: July 12, 1985 * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison

Park, PA, were used.

<Continued)
Report 48-136

Page 18

Table 4

(Continued)

Individual Animal Body Weight Data (0.0087 mg/L Aldicarb Female Rats~)

Aldicarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test

Animal

Number Weights (g) at Day 0 Days to Death

85-17083 210 During exposure

85-17084 218 During exposure

85-17085 207 During exposure

85-17086 228 During exposure

85-17087 203 During exposure

Mean: 213 Mortality

Standard Deviation: 10 Ratio: 515

Group Observations: Clinical signs observed during the exposure were ataxia,

tremors, an increased respiration rate, hypoactivity, lacrimation, exophthalmos,

and a red periocular discoloration. Mortalities occurred between 25 and 240

minutes of exposure.

Exposu~~ Date: July 25, 1985

Albino rats (ZM/Sprague-Daw1ey), Zivic Miller Laboratories Inc., Allison Park,

PA, were used. 1, )

(Continued)
Report 48-136

Page 19

Table 4

(Continued)

Individual Animal

A1dicarb Solution

ht Data (0.0060 m IL Aldicarb Female Rats*)

LC50 Aerosol Acute Inhalation Toxicjty Test

Body Wt. Change

7 14 Days to Death

During exposure

1

-8

-11

-10

2

14

11

12

2

14 (S)

14 (5)

1

.' Mortal!ty

Ratio: 3/5

Group Observations: Clinical signs observed during exposure were ataxia and

tremors. Following exposure, ataxia and tremors were not observed; however,

additional signs observed following exposure were periocular, perinasa1,

perioral, and urogenital wetness, "decreased motor activity, and a decreased

respiration rate. During the postexposure period,signs observed were

periocular, perinasa1, and perioral encrustation, discolored and unkempt fur,

decreased motor activity, a slow mid-air righting reflex, abdominal breathi~g,

and a decreased respiration rate. No clinical signs of toxicity were observed

on postexposure days 5 through 14.

Exposure Date: July 31, 1985

(S) = Sacrificed on August 14, 1985

* Albino rats (ZM! Sprague-Dawley), Zivic

Park, PA, were used.

Miller Laboratories Inc., Allison;,'J

,

",;

-:...~.

(Continued)
Report 48-136

Page 20

Table 4

(Continued)

Individual Animal Bod Wei ht Data (0.0020 m /L Aldicarb Female Rats*)

Aldicarb Solution (in DCM LC50 Aerosol Acute Inhalation Toxicity·'TeS't

Animal Weights (g) at Day Body Wt. Change

Numbel: 0 7 14 7 14 Days to Death

85-17104 234 244 280 10 46 14 (5)

85-17103 226 213 259 -13 33 14 (S)

85-17105 229 240 247 11 18 14 (5)

85-17106 239 226 271 -13 32 14 (5)

85-17107 236 245 283 9 47 14 (S)

Mean: 233 234 268 0.8 35 Mortality

Std. Dev. : 5 14 15 13 12 Ratio: 0/5

Group Observations: Clinical signs observed during exposure were ataxia and

tremors. Additional sign& of toxicity observed following expoe~re were periocular, perinasal, peroral, and urogenital wetness, decreased motor activity,

abdominal respiration, and a decreased respiration rate. During the postexposure period, signs observed included periocular, perinasa1, and perioral

encrustation, decreased motor activity, abdominal breathing, and a decreased

respiration rate. No clinical signs of toxicity were observed in two animals

by postexposure day 1, in 4 animals by postexposure day 2, and in all survivors

on postexposure days 6 through 14.

Exposure Date: July 30, 1985

(5) C Sacrificed on August 13, 1985 * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison

Park, PA, were used.

(Continued)
,"R:~,p'oit 48-136

." Page 21 "

Table 4

(Continued)

Individual Animal Body Weight Data (0.00082 moIL fi~U~Ca[O Female Rats*)

Aldicarb Solution (in DeM) Le50 Aerosol Acute Inhalation Toxicity Test

Animal

Number

Weights (g) at Day

o 7 14

Body Wt. Change

7 14 Days to Death

85-17099

85-17098

85-17100

85-17101

85-17102

Mean:

Std. Dev.:

217

224

221

244

211

223

12

217

232

231

255

212

229

17

261

271

265

290

244

266

17

o

8

10

11

1

6

5

44

47

44

46

33

43

6

14 (5)

14 (5)

14 (5)

14 (5)

14 (S)

Mortality

Ratio: 0/5

Group Observations: Clinical signs observed during exposure were ataxia and

tremors. No clinical signs of toxicity were observed following exposure.

During the postexposure period, clinical signs were observed only on postexposure day 1. The clinical signs observed were periocular, perinasal, and

perioral encrustation, urogenital wetness, discolored and unkempt fur, and

decreased motor activity.

Exposure Date: July 29. 1985

(S) = Sacrificed on August 12, 1985

* Albino rats (2X/Sprague-Dawley), Zivic Mill~r Laboratories Inc., Allison

Park, PA, were used.

WPC/rkk/0909B-3

11-11-85
... ()R.r~~NSIF).n.d.l.ngs _

NASAL CAV ITY. Nl;L

IPerill<l!;;! I elll:ruslat ion

/Perinasal staining

TRACIII-:A, NGL

/Mucous f HIed

LUNGS. NGL

/Colllr change

BRONCHI, NGL

I.J VEl{. NGL

IC(,lur chiln~e

KI DNI-;YS, NGL

IlIyd~lll\eph~o~is

/Cyst, cortical su~face

C~~RV ICAL I.YMI'H NOlll~, Nl;L

ISize change

/eo I or ch;ll\~e

EYES. N(;L

IC"rl1('ill ('pi!e I ty

·/J.entlculil~ upncity

. /l'e~inclllilr cncrllstntion

II'eriocu1.1r stilll1ing

Nt:!. = No gross lesions.

Table 5

Aldicn~b Solution (in DCM) LC50 Aerosol

Acute Inhalation Toxicity Test

F~equency of Gross Findin~ for RatH that Died

Mnles -------

~gj.llle~~_I.~I}_~·.;I_rj?

O.04h3 0.OO!l7 0.0060 0.0020

5/';* 20 1/2 0/1

0/5 1/5 1/2 0/1

0/5 2/5 0/2 1/1

5/5 5/5 2/2 1/1

0/5 0/5 0/2 0/1

2/5 0/5 0/2 1/1

3/5 5/5 2/2 0/1

5/5 S/5 2/2 1/1

5/5 5/5 0/2 1/1

0/5 0/5 2/2 0/1

4/5 3/5 2/2 0/1

1/5 2/5 0/2 1/1

0/5 0/5 0/2 0/1

S/S 4/5 2/2 1/1

DiS 1/5 0/2 0/1

0/5 0/5 0/'2. 0/1

0/5 0/5 0/2 0/1

2/5'; r./5 O/Z 0/1

4/5 5/5 1/'2. 0/1

2/5 1/5 2/Z 1/1

1/5 4/5 0/2 OJ]

Females

~/liter of Aldicarb

0.0463 0.0087 0.0060

5/5 4/5 3/3

0/5 1/5 0/3

0/5 0/5 0/3

5/5 5/5 2/3

O/S 0/5 1/3

2/5 0/5 1/3

3/5 5/5 2/3

5/5 5/5 3/3

5/5 5/5 3/3

0/5 0/5 0/3

4/5 4/5 3!J

1/5 1/5 0/3

1/5 0/5 0/3

4/5 5/5 1/3

1/5 0/5 1/3

0/5 0/5 1/3

0/5 0/5 0/3

2/5 0/5 O/)

3/5 5/5 2/3

0/5 115 2/3

4/5 3/5 0/3

*Numeracor equals number of rats with specified finding.

1ll!llllminntor CI!U11lS numher of r:lts for which specifi(',t orgnn was rxnmined.
Llh I .. ()

Aldicarb Solution (in DCM) LC,50 Aerosol

Acute Inhalation Toxicity Test

.r:..r~(~~l!.I1:~y_.!~r_Cross fl~~IJ!~;_~\.:lts ~~ r1 f iced at 14 [)ays

Mill!!5 Females

mg/liter of Alclicorh mg/llter of Aldicarb

ORGA~S/Flndings O.OOhO O.OO:.!O O.UOU82 O.OOhO 0.0020 0.00082 ------

NASAL CAVlTY, NGL J/3* 4/4 5/5 2/2 SIS 5/5

TRACHEA, NCL 3/3 4/4 5/') 2/2 5/5 S/5

LUNGS, NGL 3/3 3/4 5/5 2/2 5/5 5/5

/Color change 0/3 1/4 0/5 0/2 0/5 0/5

BRONCHI, NGL 3/3 4/4 5/5 2/2 5/5 5/5 _

LIVER, NGL 31J 4/4 5/5 2/2 5/5 5/5

KlDN~:YS, NGL 3/3 0/4 3/5 2/2 5/5 3/5

/Hydronephrosis 0/3 4/4 2/5 0/2 0/5 2/5

CERVICAL LYMPH NODES, NGL 3/3 J/4 4/5 2/2 4/5 - 5/5

/S i?e Cll<ln~e 0/3 1/4 1/5 0/2 1/5 0/5

EYE, N(;L J/3 4/lj 4/5 2/2 5/5 5/5

/}.cnt il:~~)a f{-'pae i ty 0/3 0/4 1/5 0/2 0/5 0/5

-- -

Nl;!, ,=. No RrosH lesions.

*NlImerator equals 1IIIIIIher of rllts with !Jpecified finding.

lll!llu1l1inatur f.lqu.l!s l1u1I11>l'f of rats fur which specified org,ln was examined.
Pressure Gauge

& I{l'glilator

.j.

l'i~\lIre I. Al'rllSlll Cl'nc'r:ltillll and I~xpll:-;ure System

UseJ for till! AIJil';lrb (in IJU-lj LC50

Acute Inhalatioll Toxicity Test

'-FMI Pump

+Snmple Reservo

+ Snmp le

+ Magnehelic

]:

~·LiJ:I~~ ~(l \111 ::0 x

+ Ai r I'istmli I

......Ht-1------l

Ch:llllbL' r .~ ." V

I t. tmp lllgers Filter

Cagp.H
50 -i-Uu.wWU++++W-~H-H*H+ttltt1fttit-jHttttttttttiittttttlHitI1tttl

40 ._. _ .

30

9999 999 99'

F i )'.\11"1" ~~

1\1dicarb Solution (in DCN) Part kll! Sl.w Ilistrl.hutloll - O.OOB? mg!], 99 98 9~ 90 80 70 60 ~o ~o 30 10 10 1 o 01 01 o O~ 

., 

1j.lU",1 LJIU

.,.::-.t-·II 20 OlUll4-WJWUUlHffi-t+tt+HffittH-H-OOrnt11rttHtHitHHttttttHtttffitfttfmtttttm~tttMr

TIT

ifT

:~:.c. t:J: .

i

I

:1

:: ,..

"1

.!

:=T'I""l 1: " .1< Ill=l-c .,' :n,

'l~r

IIII

'1:::' "t=.: · IHlt';-tlf=!ill

•.!"I11I]-

.... -l--

.II -"f -+-

.. I

."

I

llil

-+-++ lll»~~

·'···r~·t·+/IIIII-:-IT' " •• 11 ..

:I

4

.J. ". .', I I' J I . - .. 1.:.. := l- j; lj.J.q:.- r Ifi~ .

lOt .·llltllI·,.,·IIIIt."I:-:.···· *.~::: ~:.II:: li-·~-~iill.

.. ! -+Tt·mmtWmtmtt.tl:l:ttjj:jtJjj:

O B

I ,. I ' ' .. ·f

I ill I I .. .... 

I I. 1 !II!

10

V1 - 8.0 .~

·w

..... 6.0 ,·w

S 5.0 ~.

U 4.0 ,Or-'.

::c

--- 3.0 o' W·N

. 2.0 V1

0.6

0.5

Q

3.4

14.0

14.0

T4

998 999

o~

D50

084

98 99 9~ 20 JO ~o ~o 60 10 60 90

Percent by weight less than size

\0

1)

o

ill.

o O~ 0 I 02 0.01

\.J JIIITI
• Ill'

05 02 01 OM

f--:-1-H--HIIIt-t- t-

·11

If

III

! I !i

II

IT

n.

iIH't:r-

-Hwt:,:-

.'1,

II

Vti-=Fjl

..-1-,--

l=

Figure 3

Aldicarh Solution (in DeM) Particle Size Distribution - 0.0060 mg/L

91 95 90 10 70 60 50 ~o 30 20 10 2 I 99

~-l9999 999 991

50~1111'11I111I1 I IIIIII11111

I I! 1111111 II

40

30 -I I I III

_.....

. 

:~4-HHtH-

D50 ... 3.6

D84 = 9.0 

9.0 

og = 3.6 = 2

1--

~\-:.

..., .....

1-l-~-W-I-l·UUl~l W-I-l-f+I-l·U·l-l-t+ml-H-j+H-~ ~-+++-t--l---f.:-

'0 '0 10 "0 tn 60 '0 eo .0 _._ .... ~.a...a-. bM. ......,..L.4&~.. "'•• " •• ,.J- 

.-. -"

• nl Q ..

~~':O.~ ·H'Ttttttlt+Ht-+-H+Ht++H-l-II-*+-WW-WW

i~~b~:.gt:rttttttih~f+iHij~H-H--++-J~+++~ iO;3 .-- ;.~;, .

~~~O:2; <)) ::.'.~

f;:~~:~~~-j-' .

:KA

."',j';_''-, ".'-0;
"" '" '"~

ri "\Ill' :1

Aldi";lrh Sl1lutioll (ill Ilcm l'nrlil'll~ Si~,l' lliHlrihulill1l - O.OO:W 1lI1\/1.

"9~ 95 90 10 70 bO 50 AD JO 20 10 05 01 0 I 0 

50 -f-+-i-+H-fHf-+T-+-t-H+Ht++t+t-:t-+-I-H+H+HtI

40

30

II

!I

II

iir-

!i1,

lilT

'I:

lIT . ..-.

r, Il!~.

I: HIl

99. "'

+--;J~:~l

i-tlH-

-~H:'::

D50 = 3.8 

D84 = 15.0 

15,0 

og = 3":B == 

."1"

... ~-I-t-

-r=

II

li!l!

1111 i f 1':1' I •• 1-

Iill i

I

ll' 'I ' .' I ,. " hI i 'i-:~ -r- h;;

~-l

Ii

" 99

-

---r-'J'

.-t-. I

f- ,.

I!

•• ~. 1="

.t~ -~i=tt-t

1=.:1::

.'-1-+--

95 20 JO AD 50 60 10'0 90

Percent by weight less than size 10

T

5

_ ..;;jL.

I =:J2

1.1

05

.....:....

02

I=tr..

H HIII--+t-++H-HH-HHb/l-f:k

-fI-.:.:+-I-++HIII-H-' -

;0 • __J1I

20

10,.,_•.••

¥:j~ ~l2Emt+tf1ffiHtlititt=+Hf 2L' s.o, '"-'---'~-. ~e', ',,- 'U,-. 4.0 - _

~:'3.0 . 111111111;1 ~,~.'.' ·'~lJjllllllll, j :iii' 2.0 .....
. ': ill I : ! I! nn i

Illli! J I I II lil!1 

999 WI 99

figure 5

Aldicilrb Solution (in OeM) Particle Size Distribution - 0.00082 mg/L 91 9~ 90 80 10 00 ~o 40 30 10 10 • 1 I O~ 01 0 I 0

1,·1

,:1: Ii, I IiI!i I !iii ! : I i 11 il i 

_·_··-I!IHIII-H-H+I+ I~Wj

•• , • ,•. 1

I

j'-- "WU4-HttH:"j' -H-t~III::I: '. -: dt 0"' I I I _•. , 

.~liitLH!i iI[ Ii. ma

-I' . 11+1 !'l;Q"I,--l -r+- mgt 

: ::: ,I Till;' •-t' :r.... ,: 

I l, t I t- ... -- '''1 

-tWI: ..

tHI.·. " ' ; 10.,.., .

. '80 I .••

>~i , ,'. II III

{~~ '6.-0 II, III

f!', ,~;6+-+-H-I%Hf++-++t+tti \~,l;O

icil . ' :,·.N.

~::~~'"

.( . i I I i I'IIIIIIIIH--t-l-I-I+HHH+

" . • I I

lI~rlllllllllllllllllllllllllllll

050 = 2.0

D84 = 10.0

10.0 11 g = -T:f) = 

........-/-+-

1/ '-~l'

__ . . .~.,.,~.,:>~.~,;:;J,i.::,-:;.~_";, -: ,~-.:

,<0«'.

:V~i:1~ ~~..~.-.
R. O. 4, Mellon Road, Export, Pennsylvania 15632 •• . : I .,.

BUSHY RUN RESEARCH

Report 48-136 Pilg~' .!':!

CENTER

Telephone 14121 733·5200

Compliance with FIFRA Good Laboratory Practices

This study was conducted in accordance with current Federal Insecticide,

Fungicide, and Rodenticide Act (FIFRA) Good Laboratory Practices (GLP).

\'"PC/rkk/0909B-2

10-03-85

D. J.

Study

I

, i" ,. . ' .. .r..,,..........:.~ 1~ 0 __

Nachreiner, B.S.

Director

f

i:

Date
Report 40-136 •.

Page 3u

•

R. D. 4, Mellon Road. Export, Pennsylvania 15632

,,"

. : ..

~.,

BUSHY RUN RESEARCH CENTER

Telephone 14121 7:13-5200

Qualitv Assurance Unit Studv Inspection Sumroarv

rest Substance: Aldicarb in DC~

Study: LC 50 Acute Aerosol

Study Director: D. J. Nachreiner, B.S.

The Quali:y Assurance ~nit of BRRC conducted the inspections liste= c~2~~

and rep=rted the res~lts to the study director and to management on the ~a:fE

incicated. It is the practice of this Quality Assurance Unit to report t~~

re5~lts of 'each inspection to both the study director and management.

1:15:-<.'::::ic;'1

Da:e

Date QAL Re~~rt Issue:

To St~~\ Direct~~ 2~ X~~az~~=~:

12-20-83 Standard Protocol and

Amendment

12-20-83 1-26-84

7-2-85 Standard Protocol Amendment 7-2-85 7-8-85

10-9 to

1Q-U-85

Final Raw Data and

Final Report

10-11-85 12-9-85

. ' ..' '. .'

/). '. . ',',' "':.~:'.~"

.;....••·.~ti~~~;~~i~~_'j~illt~ ,....::.,~'::d;"7,;~}?.t.,:~.~ ..~~~.{~~c~,~~~~~,~;:k2i~~~x§1~~~~~ f~i:[~t)'J~;,~~~~~{{ .'. '
H"[h1 r l 8- 1 ...

APPENDIX 1

Exposure Chamber Atmosphere Analyses for the Aldicarb Solution (in DC~)

Acute LC50 Aerosol Inhalation Study on Rats

(9 Pages)
Rt>pClrt "R-136 '

APPENDIX 1

Exposure Chamber Atmosphere Analyses for the Aldicarb Solution (in DC~)

Acute LC50 Aerosol Inhalation Study on Rats

SUMMARY

The concentrations of aldicarb, dichloromethane (DeM), and methyl

isocyanate (MIG) in the exposure chamber atmosphere were determined during t~e

five exposures by chromatographic methods. The aldicarb was scrubbed from t~e

chamber atmosphere using impingers; the solutions were then analyzed by reve=se

phase liquid chromatography. Dichloromethane and methyl isocyanate

concentrations were determined by direct analysis ,of the chamber atmosphere

usinJ gas chromatographs equipped with flame ionization detectors. A gas

chromatograpt equipped with a nitrogen-phosphorus detector with sensitivity to

detect ppb concentrations of methyl isocyanate was used for industrial hygieuc

monitoring.

The mean aldicarb concentration (± SD) for the five exposures were 0.82

(± 0.100), 2.0 (± 0.15), 6.0 (± 0.61), 8.7 (± 1.38), and 46.3 ug per

liter.

The mea~ dichloromethane (DCM) concentrations (± SD) for the same

exposures were 12.7 (! 5.76), 27.7 (± 2.71), 54.0 (= 5.19), 7.4 (= 1.4B),

and 49.1 (= 12.97) ppm. These dichloromethane concentrations are equivalent

to 43, 94, 183, 25, and 166 ~g per liter.

~o methyl isocyanate was detected in the chamber atmospheres. The

detection li=it was 0.9 ppm.

CHEMICALS

Aldicarb in Dichloromethane (DCM) Test Material: BRRC Number 48-121 A and B,

HrS5819;_~. Formulation containing 37% aldicarb, 59% dichloromethane,anc

5000 pp~ ~ahimum methYl. isocyanate (MIC) and provided by rnion Carbide·

Agricultural Products Company.

Aldicarb: 2-metnyl-2-(methylthio)-propionaldehyde o-(methy1 carbamyl ox:=c)

Aldicarb Standard: BRRC Number 48-117 A and B, HAS3813AA. A 99.4',

a1dicarb standard provided by Union Carbide Agricultural Produ~ts Compa~y.

Methyl Isoc;anate Standard: VICI Metronics (Santa Clara, california) standa~:

rate per~eation tube. Part Number 110-150-7473.

Di.ch1oromethane Standard: BRRC Number 46-283.

reagent.

Acetoni trUe :BRRC Number 48-..71,

\o'ater: Fl1teredwater obtained .," :'
th.';"'(lrt _":,-: ','

APilL':H! i :.: :

-2-

INSTRUHENTATIOl':

Aldicarb Analysis

High pressure liquid chromatography was performed using a Spe~tra-Phys1cs

SP BODO B liquid chromatograph equipped with an SP 8400 ultraviolet/visible

detector and an SP 8000 Data System.

Dichloromethane Analysis

A Perkin-Elmer Sigma 2000 gas chromatograph eGC) equipped with aflame

io~ization detector was used to monitor the DCM vapor concentration in the

chamber atmosphere. Peripheral equipment included a VICI Va1co Instruments

automatic gas sampling system and a Spectra-Physics SP-4200 Computing

Integrator •

~ethyl Isocyanate Analysis

A Perkin-Elmer 3920B gas chromatograph equippeJ ~ith a flame ionization

detector was used to monitor the MIC concentration i~ the chamber atmosphere.

A Spectra-Physics series 4000 central processor, a data interface, and a PerkinElmer automatic gas sampling system were used for the analyses. A VICI

~etronics Model 450 Dynaca1ibrator was used to prepare MIC gas standards.

The MIC hygiene monitoring was performed using similar instrumentation to

that used for the MIC chamber atmosphere analyses except that a

nitrogen/phosphorus GC detector was used instead of the flame ionization

detector.

ANALYSIS METHODS

Aldicarb

The reverse phase high pressure liquid chromatography method is summarized

as follows:

1. Colultn:

2. Mobil~ PhsBe:

3, Flow Rate:

4. Injection Volume:

5. Ultraviolet Detection:

6. Flo\-; :-lode:

7. Preco1umn Ct!'ard Cartridge:

8..Retention Time

A1ltech Rosil C~ BUReversecPhase

(250 x 4.6 mm)"'

60% Water/40~ Acetonitrile (v/v)

1. 5 mL/minute

10 ).lL

210 nm

Constant

Brownlee RP-18 SPHERI,..5 :-IF,LC'',

.3 "C'!!LX 4 ,'6 'mm' "~"

5 minutes
i-:l' ;", r: ;.,;.- I 3i)

1\1'1',-,11,: i ;.: I

-3-

Dich]oromethan€'

The gas chromatographic method is summarized as follows:

1. Col umn:

2. Column Temperature:

3. Gas Sample Valve TeJllperature:

4. Flame Ionization Detector

Temperature:

5. Nitrogen Carrier Flow:

6. Gas Sample Loop:

7. Retention Tim€':

Methyl Isocyanate

10% SP2100 on 80/]00 mesh

Supelcoport C (6 ft. x 1/8 in.

stainless steel)

60·C

60·C

20 mLlminute

1 cc stainless steel

0.91 minute!':

The gas chromatogra?nic method is summarized as follows:

1. Col u!!;n: Chromosorh 101 (3 ft. x 1/4 in.

stainI ess steel)

2. Colul::1 Temperature: 135· C

3. Gas Sample Valve TetJpera ture: 150·C

4. Flal:E Ionization Detector

::-e:::perature: 200·C

5. Nitrogen Carrier Flo\..': 20 m~/minute

6. Gas Sample Loop: 1 cc stainless steEl

7. Retention Time: 129 seconds

The MIe ~ygiene monitoring gas chromatographic ~ystern had similar op€'r2::~~

parameters to those of the methyl isocyanate chamh€'r atmosphere analy~is

parameters. The nitrogen/phosphorus detector was o..erated in the nitro~er c~:~

and the carrier flowrate was 40 mt/minute. A 4-foot Chrolllosnrh 10] column ~~~

used.
K\·;1,,-... r t !. ~~-

/·.;JrlVfhJ i ;.: !

-4-

CALIBRATIO~

Aldicarb

Two analyses vere developed; one analysis for high concentrations arid onp

for lov concentrations.

High Concentration Aldicarb Calibrati0n (Attenuation Range = 0.16)

A stock aldicarb standard solution vas prepared by weighing the

aldicarb standard into a volumetric flask and diluting to mark with

acetonitrile. Solutions ranging in concentration from 0.1 to I mg/mL were

prepared by diluting the stock solution volumetrically. Ten microliter

volumes of standard solutions were injected into the liquid chromatograph.

A linear calibration curve (Figure 1) was obtained when peak areas were

plotted versus concentration. The calibration was checked each exposure

day.

Low Concentration Aldicarb Calibration (Attenuation Range = 0.0025)

A stock aldicarb standard solution was prepared by weighing the

aldicarb standard into a volumetric flask and diluting to mark with

acetonitrile. Solutions ranging in concentration fro~ 0.001 to 0.010 wg/~:

were prepared by diluting the stock solution volumetrically. Ten

microliter volumes of standard solutions were injected into the liquid

chromatograpt. A linear calibration curve (Figure 2) was obtained when

peak heights were plotted versus concentration. The calibration was

checked each exposure day.

::~chlorclLet:'ane

Calibration of the gas chromatograph was achieved by injecting gas

standards, which were prepared by syringe injection of test material into

,edlar* gas bags. These standards were prepared using the mathematical

:'"clationship:

v

C x V

h x MW x 298 x P

d x 24.45 x T x 760

where: V required volume of calibration liQuid"in milliliters (mL) at

temperature T in degrees Kelvin (OK)

C the desired final calibration concentration in parts per

tr.illion (ppm)

Vh = volume of container (Liters)

MW molecular weight of the calibration liquid

P barometric pre~sure in millimeters of mercury (mmHg)

d density of calibration liquid in grams per milliliter (g/~L)

at temperature T

24.45 molecular volume at 298°K and 760 woHg, in Liter~

T temperature in degrees Kelvin (OK)

* Registered Trademark, DuPont de Nemours, E. I. &Co.
Rl'p,,~: ~-;-I y,

ApPl'l~C~ i :.: I

-5-

A linear calibration curve was obtained when areas (integration counts)

were plotted versus the gas standard concentrations. Tlte calibration factor

(KF) was calculated as:

AREA

KF = CONCENTRATION

The mean KF for all valid injections of standards was used in subsequer.t

microprocessor calculations.

Methyl Isocyanate

Calibration of the gas chromatograph was achieved by dynamically

generating gas standards which were prepared using a VICI Metronics standar:

rate permeation tube and a VICI Metronics Dynacalibrator 450. The rate 0:

methyl isocyanate permeation from a 15 cm permeation tube equilibrated at 50"r.

was determined graVimetrically to be 10351 ng/minute. Individual measureme~ts

were 10099, 10709, and 10246 ng/minute. Gas standards of different

Loncentrations were then prepared using the following relationship:

C = R x K -Fwhere: C

R.

F

K

concentration (ppm, v/v),

diffusion rate (ng/min),

Air flow rate (mL/min),

reciprocal vapor density of material in nl/ng.

A linear calibration curve was obtained when peak heights were p10ttec

versus the standard concentrations. Peak height measurements extended the

linear calitration range (0.9-13 ppm) to the minimum detection limit of

0.9 ppm.

Calibration of the gas chromatogr:.?h for the MIe industrial hygiene

monitoring system paralleled the calibration methods used for the MIC cha~:er

atmosphere monitoring system. A 4.0 cm permeation tube equilibrated at,4C=(

yielded a 14il ng/minute permeation rate. The calibration standard

concentrations ranged from 35 to 1170 ppb. A linear calibraticn curve was

obtained when peak areas were plotted versus HIe concentr.ation.

C~~BER ATMOSPHERE ANALYSIS

Aldicarb

Two mi~get impingers (in series), each containing approximately IS m:

acetonitrile, were used to scrub the chamber air samples. Impinger

efficiencies were 97% or grea~er. A Terracon pump with an in-line critica2

orifice, ope~ating at a f10wrate of approximately 700 ~L per minute, was u5e~

to sample t:;e chamber atmosphere. The sampling system, flowrate was calib::a,:e':

using a Singer dry test meter. Samples were collected for 10 to 55,mint~~s;

depending on chamber atmosphere concentration. The teflon samp::'ing lin,~I0;~5

washed wit:: acetonitrile after each analvsis. The acetonitrile /aldicarb·"·..i.;;

solution was analytically transferred to' the front impingersoluti,on;,a.nd" ",:;"

included in the analysis. .
RL';l\lrt :.~-: j'-

:\:';1';":11<\ i :'. :

-6-

The least sensitive analysis method (Figure 1) was used for th~ 46.3 ~g

per liter exposure. All other exposure analyses made use of the more

sensitive method (Figure 2). The test material used in the 46.3 and 8.7

Ug/L exposures was undiluted; for all other exposures, the test material was

diluted ten-fold (w/w) with dichloromethane.

The concentration of aldicarb in the chamber atmosphere was calculated as:

C

LCC x VI x 1000

VL

where: C Aldicarb Chamber Atmosphere Concentration, ~g/L

LCC Aldicarb Chamber Concentration of the impinger solution as

det2rm1ned by liquid chromatography, mg/mL

VI Impinger Volume, mL

VL Volume of chamber air scrubbed, L

~ote: If the impinger efficiency was less than 100%, both impinger solutions

were analyzed aud included in the analysis calculation.

The mean aldicarb conc~ntratlons (± SD) for the five exposures were 0.82

C~ 0.100), 2.0 (~ 0.15), 6.0 (± 0.61), 8.7 (± 1.38), and 46.3 ~g per

liter. See Table 1 for a summary of the analytical data.

Dichloromethane

DCM was sampled directly by the GC automatic gas sampling system. The

chamber atmosphere was analyzed for DCM at least 40 times for each full 4-hou~

exposure period. The mean DCM conc~ntrations (± SD) for the five expcsures

were 12.7 (± 5.76), 27.7 (± 2.71),54.0 (± 5.19),7.4 (± 1.48), and

49.1 (± 12.97) ppm. These DCH concentrations are equivalent to 43, 94, 183,

25, and 165 ~g per liter. The DCM analytir.al results are summarized in

Table 1.

~ethyl Isocyanate

HIC was sampled directly by the GC automatic gas sampling system. The

chamber atmosp~ere was analyzed for MIC for the 46.3 ug/L aldicarb exposure

5 times and 26 times for the 8.7 ~g!L aldicarb exposure. No MIC was·

detected. The minimum detection limit was 0.9 ppm. For the other three

exposures, the MIC analyses were discontinued since the further dilution of

the test material would decrease HIe concentrations by another factor of ten.

l .---

Date

lftPC/rkk!0894B-4

10-14-85
Rt.:p.',rt :.:;.:-: 'J1

Appt"n';! ;-; J

-7-

Table 1

Analytical Results Summary

A. 0.82 ~g/L Aldicarb Exposure

Aldicarb Concentration, H&/L Dichloromethane Concentration, pp~

0.68, 0.90, 0.88, 0.80

Mean: 0.82

SD: 0.100

B. 2.0 ~g/L Aldicarb Exposure

Mean:

SD:

Samples:

Range:

12.7

5.76

44

3 to 43

A1dicarb Concentration, ~g/L Dichloromethane Concentration, pp~

1.8, 2.1, 2.1, 2.1

Mean: 2.0

SD: 0.15

C. 6.0 ~g/L Aldicarb Exposure

Mean:

SO:

Samples:

Range:

27.7

2.71

44

22 to 34

Aldicarb Concentration, ~g/L Oichloromethane Concentration, P?~

5.8, 6.9, 6.3, 5.9, 5.3

Mean: 6.0

SD: 0.61

D. 8.7 ug/L Aldicarb Exposure

Mean:

SO:

Samples:

Range:

54.0

5.19

40

44 to 64

Aldicarb Concentration, ~g/L Dichloromethane Concentration, F?=

9.9, 9.4, 9.8, 8.7, 7.9, 6.3

Mean: 8.7

SD: 1.38

E. 46.3 ~g/L Aldicarb Exposure

Mean:

SD:

Samples:

Range:

7.4

1.48

44

4.2 to 11.4

Aldicarb Concentration, Ug/L Dichloromethane Concentration,

Mear.:

SD:

46.3

46.3

Mean:

SD:

Samples:

Range:

'..9.1

12.97

9

23 to 65

WPC/rkk/0694B-3

10-15-85
•

2258.

2iHlfl.

175iL

1::5~.

e.

Eo. I

0. 2

Rt.·;... ·,rl ':'K~ l ),.,

ApI"'IHI i :.; I

-8-

Figure 1

Aldicarb Calibration Curve

./

/,

.....I--~----I---.---....,...i---'---,'

e. S £1.7 LL·~

MG/ML ALDICRRB~~
R"'l'.'rl :K-\ 36

Ajlpl·nd i :.: I

-9-

Figure 2

Ald1carb Calibration Curve

2.

r--r---,r-----.---.j---.----y-,---r-,---"r----"T-...,--,I: .'

4. 6.1 8.11'iL1

1.88.

90.

p 8e.

E

R

K 78.

H

E 6e. 1

G

H

T SB.

M 4B.

rl

3B.

2B.

1&.

0.

B.
APPE:\DE 2

Individual Animal Pathology Data

Aldicarb Solution (in DCM) LCSO Aerosol

Acute Inhalation Toxicity Test

(5.0 Pages)

Report 48-136
•

Test Name: Acute Acro~()1 Jnh.Jl.Jt ion Tn:-:icit·.. (I.l'>\"

Path. P;lft' "1

Test 1'0.: 14

PAT H 0 LOG y

RL'i~l)rt :.;-'-1 :,'

APPL'lld i ;.; .'

Sample No. : 48-121 A Species: Rat Animal />;0.:

Sample Name: Aldicarb DC~l Strain: Z~l/S-[)

Treatment Level: 0.0463 mg/liter Sex:

Fate: -X-D E EM

Kodachrome: Gross

Female

Date of Death:

~1i c ro

Age: 8-~ ~cc~e

7/12/85

Clinical Histor, '-

= -

Gross Findin~s

I Date T::':',_€~

- Red stained, bilateral. i /12/85 : : ;J_~ .~_\:

- Ri~ht - Corneal opacity. Or..'an ....:~-L.. 1£) - Liver

Lungs - ~kttled light and dark pink, all lobes. K:cne\'s

S::leen ;

Right kic:-.e\' - 10 x 5 IlL':: clear fluid filled cyst on Heart :

cortical suriace; inadvertantly cut long. Brain - Severe hydronephrosis, bilateral. Pitui'tar,\'

Bronchi

:i.crenals

Xasal ca\'i t\', Trachea, Liver, and - No gross GO!lads ' lesions. I Eo='; I....eight·

D - Sponta:-.e;)~s
:a:::. P:lf.e /12

PAT H 0 LOG Y --------

R... p"rt :'.<;-1 :I"

APlk'''cJ i :.: '

14 Test Name: Acute Aerosol Inh~]ati0n Tnxicitv (LCSUJ

'=-"-"-"-'---

Sa:::?le No.: 48-121 A Species: Rat Animal t-;o.: 8':,-167E3

Sa=?le Name: Aldicarb DeM

7reatment Level: 0.0463 mg/liter Sex:

Strain:

Female

ZM/S-D

Age:

fate: -lLD E P.1

Kc~achrome: Gross

Date of Death:

~li cI'D

711 '2/85

Clinical Ristor, .....

I

I

I ;', Gross findings I

I

I I Date I Ti:::e 1 - Lenticular opaci:y, red stained, bilateral. 7/12185 - I I 11: 36 ;...:..:

i OrDan I '.-;L_ (c-)

i - Mottled light and dark red, all lobes. Liver I 1

I :;::'5 a 1 cavity. Trachea, Liver, Kidnevs, and Bronchi Kidne\"s i - Ko 5nleen I

! gross lesions. Heart I

I Brain i

1 Pituhar\" ! I

! Adrenals ,

I

~cs I

-- Boc'\; ;"·eight I I

:
Path. P~1r," " 3

R\.'!)l'rt ':',",- J j1i

Appt::h'Ji:.: '.!

Test No.:

Sample No.:

14

48-121 A

Test Name: Acute Aerosol ]n11Lllatjon TQ~;icit\' r!.C:'(11

Species: Rat Animal };o.: 8:;-1>';".:.

Date of Death:

!'1icro

Sample Name: Aldicarh DCM

Treatment Level: 0,046) mi:/liter Sex:

F'ate: -X.....D E EM

Kodachrome: Gross

Strain:

Female

ZM/s-J)

____---'7.1l.'2.<-I..... c_, _

Clinical Historv

Gross Findin s

Right - Corneal opacity.

- Left - Red stained.

Cervica! lvmph nodes - Enlarged 3X normal.

Lungs - Mottled light and dark red, all lobes.

~as21 cavity, Trachea. Liver, Kidneys, and Bronchi - ~o

gross lesions.

1====;~D~:!:e'-====~==iTb'.::..,:::_e""._;-,.=,=,,!

711'2/85 L::u :\.."

Or an

Liver

Kidnevs

S leen

Heart

Brain

Pituitan'

Adnmals

Gonads

Bod...· "'ei£::'t·
Acute Aerosol InhalatiC'D Toxicit·,. (LC:"(I)

Path. r.lf.<'

rest ~o.:

Sample No.:

sample Name:

14

48-121 A

Aldicarb DCH

Test Name:

Species: Rat

Strain:

R('r~'rt !.;"';-1)1

;\pj1~·n...; i :.: ...:

Animal No. :

ZH/S-D

rreatment Level: O.0463mg/liter Sex: Female Age: 8-9 Weeks

Fate: X D E EM

Kodachrome: Gross

Date of Death:

~licro

7/l2!8S

I

r

Clinical Historv

Gross Findin s ---------l

Date Tin!?'El 7Il?/P,; Il...;~.-r···

Own I ~l.... (£)

Liver

Kidnevs ,

Spleen

Heart

Brain I

PituitarY

Adrenals

Gonads == Boe, h'ei.>:~t . I

"

'.

:

~a5al ca,it\", Trachea, Liver, Kidneys, Lungs, and

Bronchi - No gross le~.

- Lenticular opacity, bilateral.
Path. P:lfl' .. " PAT H 0 LOG Y R E COR D --------- ------

Rt..'j'\ j"t _ ;"'i-: ~r\

'\1'1"'11<1 i:-: "

Test Name: Acute Aerosol Inhalation To>:icin (Lt.:"!'

Species: Rat Animal No.: 8'J-;i)7b~

Treatment Level: 0.0463 mg/liter

Test ~o.:

Sample No. :

Sample Name:

14

48-121 A

Aldicarb DC1'l

Sex:

Strain:

Female

ZM/S-D

Age: 8-9 I..'t:d:s

Fate: -.lLD E EM

Kodachrome: Gross

Date of Death:

~li cro

7/1 )/~~

Clinical Historv

Gross Findin s

Or an

7 12/8)

~asal cavitv, Trachea, Liver, Kidnevs, Lungs, and Bronchi -~L~i~v~e~r~

~~ gross lesions. Kid~Evs

S lee"

- Lenticular opacity, red stained, bilateral.

Heart

Brain

Pituitary

Acirenals

Gonads

Bod... \,eigh t !
Report 48-136

Appendix 2

Strain: .....~Z~M~/~S~-~D~ __

Fema Ie Age: _ .......:.7....:!!.w.=e.=e.!::k:.=s.....__

Date of Death: ~7~/~2~5L/~B~5 ___

Micro

R E COR D ------

Sex: --~=~----

Gross

Test Name: Acute Aerosol Inhalation Toxicity (LeSO)

Species: Rat Animal No.:· 85-17083

Kodachrome:

Path. PaCe 11 6

Test No.: __~14,,--_

Fate: -X...D

Sample No.: 48-121 B

Sample Name: Aldicarb DCM

Treatment Level; 0.0087 mg/liter

E EM

Clinical History

Gross Findings

Da.te Time

- Lenticular opacity. red stained, bilateral. 3: 11 P~1

Lungs - Dark pink. all lobes. Liller

Kidnevs

~asal cavity. Trachea, Liver, Kidneys, and Bronchi - No.

gross lesions.

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads

Body Weight
Report !oS-I3"

Appendix 2

7/25/85

Age: __-'7'---'w-'f::..:e:..:k.:.;s"--__

Strain: ZM/S-D

Sex: __~F,""e~m~a~l,",e=-- _

Date of Death:

l'licro

Test Name: Acute Aerosol Inhalation Toxicity (Le50)

Species: Rat Animal No.: 85-17084

E EM

Kodachrome: Gross

Sample Name: Aldicarb DeM

Treatme "Level: 0.0087 mg/liter

Path. Pace I! 7

Test No.: 14

Sample No.: __~4~8-~12~1~~~ __

Fate: --!-D

Clinical History 1 I

Gross Findings

Date Time

Eyes - Lenticular opacity, red stained, bilateral. 7/25/85 2:59 P~l

Orean \\'r (.,~

Perinasal area - Red encrustation Liver

Kidneys

I.ungs - Dark pink, all lobes. Spleen I

Heart

Kidnevs - Mild hydronephrosis, bilateral. Brain

Pituitarv I

Trachea, Liver, and Bronchi - No gross lesions. Adrenals

Gonads

Bod\' \\'ei~htl

.

,:':'

Prosector: H~~Stae~, , rod

D Spontaneous Death
:a~h. Page II 8

Rerh'''rt !.K-l ~tl

J\ppt,.' nd i :.:

RECORD ------

,e"t No. :

Sat::ple No.:

14

48-121 B

Test Name: Acute Aerosol Inhalation Toxicity (LC5(Jj

Species: Rat Animal No.: 85-17(J!,,,

0.0087 mg(liter Sex: Female Age: 7 wt:t::k~

E EM .Date of Death: 7(25/85

Kodachrome: Gross Micro

Sa=?le Name: Aldicarb'DCM

Treatment Level:

Fate: -LD

Strain: Z.~l(S-D

Cl inical Historv ---------..-=-

I

:

,

:

I

I

!- Gross Findings

j

I Date 1 T-i.J.le,

\ Lenticular opacity, stained, bilateral. 7/25(85 { 3:05 ?~: ! - red

I OrDan i \.:.- (-)

l"J;1~ S - Dark pink, all lClbes. Liver I

I Kidnevs i

:\asal cavity, Trachea, Liver, Kidneys, and iSronchi - :-"0 Spleen i

I gross lesions. Heart i

Brain I

I Pituitar". !

I Adrenals i

hOMads \

Bod\' '\o,'ei,"ht I

I

I

I

I

I

,

Pre-sector: .... ;

;',.'>;<,

D Spontaneous Death Euthanized E~:, .';'Euthad'z.?d ";'rro=::,~:-;~:';~~:"

• \':::'f-iJ "'\".,' . ,.§a:;."i.m~~
•

Report 48-136

Appendix 2

Path. Pace 11 9

R E COR D ------

D =.spontaneous Death E -Euthanized

Test No. : 14 Test Name: Acute Aerosol Inhalation Toxicity (LCSO) - Sample No. : 48-121 B Species: Rat Animal No. : 85-17086 ..

- Sample Name: Aldical"b DCM Strain: ZM/S-D ,

Treatment Level: 0.0087 mg/liter Sex: Female Age: 7 week.s

Fate: ..L,D E EM Date of Death: 7/25/85

Kodachrome: Gross Micro -

.,

C1 inica1 Historv

J

,

i

i

I

= Gross Findings

Date Time ,.

Eyes - Lenticular opacity, bilateral 7/25/85 2:53 P~

OrOaT> 1-1t- (p) '.

Lungs - Dark pink, all lobes. Liver

Kidnevs

Nasal cavitv, Trachea, Liver, Kidneys, and ,Bronchi - No Soleen

gross lesions. Heart

Brain

PituitarY

Adrenals

Gonads I

Bodv Wei2ht

;

, :

,

;

,.

, ,

"

;"1

-.....

, "j "•

Prosector: . ·-::~\.~5 !::t?~-l

. -~.

',,~ .~~

:,.1. .'

.. ni,d';'1 H -).•_'. c,L"
•

Report 48-136

Appendix 2

Age: 7 ,:"leeks

Strain: ZM/S-D

Sex; Female

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species; Rat Animal No.: 85-17087

Path. Pa~e 1110

Test No.: 14.

Sample No.: _.:::.;48::..-~1:.::2:.:.1...;B=-- _

Sample Name; Aldicarb DCM

Treatment Level: 0.0087 mg/liter

Fate: -1LD E _EM

Kodachrome: Gross

Date of Death:

Micro

7/25/85

I Clinical Historv

Gross Find1n2s

D"te Time

- Red encrustation, bilateral. 7'~ 2'47 py

, - Right - Lenticular opacity. Draa"" \-I.- 7.;)

Liver

Lungs - Dark pink all lobes. Kidnevs

Spleen

Kasal cavity. Trachea, Liver. Kidneys, and Bronchi - No Heart

gross lesions. Brain

Pituita!"\.'

Adrenals

Gonads

Body We12ht

'Prosector: H~fStc:t?'2 ! -~••\.J !

D = Spontaneous Death

-----,----,~._-----....;------,~.:....;.,-....;-_--::.~~~..;..,-:.'_.-:-::.:--_.-:-..,...;..:.- '.: .~.t -, ,',' ,-:" -:"", '", '"':,',,,': ',.-' :--,",- ':. " :: ,,:,:.'

Euth~,~i:ed;whe.rl'incir:.~~~t;,

;. ." ,'" "'6f77. ;.....; '... -'," ••,.... ,'•..', .. . " .,: ,:'':'?,::' .
Clinical History

...

(LC50) , ',:

10-1 I

811/85

Report 48-135

Appendix 2

Animal No.:

ZM/S-D

Acute Aerosol Inhalation Toxicity

Sex: _--,F'-.5e:JJm~a!."!l,",,e,-- _

Date of Death:

~licro

Species: __....!.R"'a....t'-- _

Strain:

Gross

Test Name:

Kod3chrome:

Path. Pane (I 11

Test No. : 14

Sample No. : 48-121 B

Sample Name: Aldicarb DCM

Treatment Level: Q.DQ6D m~/11ter

Fate: -lLD E EM

.j

. -1

!:

i

i·

Gross Findings .;.

Eyes - Red encrustation, lenticular opacity, bilateral.

Date

RillS:;

, Time _!

9: 47 A}!

Cervical lymph nodes - Enlarged 3X normal.

Lungs - Mottled light and dark red, all lobes.

Orl<an

Liver

Kidne"s

Spleen

Nasal cavity, Trachea, Liver. Kidneys, and Bronchi - ~o

gross lesions.

Heart

Brain

Pituitarv

.!'

Adrenals

Gonads :..:

Bodv Weight !.'

D Spontaneous Death '-'DI
Path. Pace II 12

Test No. : __..:1,--4:-_

Sample No.: 48-121 B

Report 48-136

Appendix 2

Test Name: Acute Aerosol Inhalation Toxicity (Leso)

Species: Rat Animal No.: 85-17172

Sample Name: _..:.A~l~d:,.=i:.::c:.::a~r~b~D..:::C.:.:M _ Strain: _~Z.:.:M!,..;/S~-....:D:..- _

Treatment Level: 0.0060 mgiliter Sex: __..::F..=e:,::m:,:::a:,.=l:.::e_____ Age: 10-11 '>leeks

Fate: ..JLD E __EM Date of Death: 7;..!/..:3::.;1'-'-/..::8~5 _

Kodachrome: Gross Micro

Clinical Historv

Gross Findin~s

D"te TimlL

7/<I/Rt; ,. "r; 1'"

J.eft eye - Lenticular opacity. Or<>an \\'r (12)

Trachea - Thick Il1.lCOUS present.

Liver '. Kidnevs I

!,asal cavity, Liver, Kidneys. Bronchi. and Lungs - No Spleen

Heart I

gross lesions. Brain I

Pituitary \

Adrenals I

Gonads I

Bodv Wei2htl

\ Prosector: ,H.'15t",,:: ."1 . , ..,c(

D Spontaneous Death

',' -.

EIJ'thanized~~en: '~C'~.i~\~~~:~'\· ".'" '~/ '., ..:.. ,.-~':~,'-><: ~'.,,>i._·c:, ..'._,~: .• '

>:~';;;i);,i,:;,:' •• >~;:~;j':.•:':~;~1J,s;l~~~
Report 48-136

Appendix 2

Strain: _-=Z~M'.L/~S.::;-D~ --:-__•

RECORD ------

Test Name: Acute Aerosol Inhalation Toxicity (LC10)

Species: Rat Animal No.: 85-17173

PAT H 0 LOG Y ---------

Sample No.: __~4~8~-~1~2~1~B~ ___

Sample Name: Aldicarb DCM

Path. PaE\e (I 13

Test No.: 14

Treatment Level: 0.0060 mg/liter Sex: ~F~e~m~a~l~e __

Fate: --K...D E EM Date of Death:

Kodachrome: Gross Micro

Age: 10-11 weeks

8/1/85

Clinical Ristor

II=============~==========l·.i Gross Findin s ii

Eyes - Red encrustation, bilateral.

Cervical lymph nodes - Dark red.

Lungs - Mottled light and dark red. all lobes.

Nasal cavitv, Tra~hea, Liver, Kidneys, and Bronchi - No

gross lesions.

S leen

Heart

Brain

Pituitarv

Adrenals I"

~~g~n~/g:.d~~~e=1=.=h=t=i======9.

,

'fl

~(;, ,.

t
Report 48-136

Appendix 2

Strain: ZM/S-D

Sex: _--:F:..;e:.;m:::;a:..l:..e=-_____ Age: __:.12::.-....:.1:.3_w::.::..E:E:::.k:;.::.s_

Date of Death: 8_/_1_4_/_8_5 _

Gross Niero

Test Name: Acute Aerosol Inhalation Toxicity (LCSO)

Species: Rat Animal No.: 85-17171

Kodachrome:

sample No.: 48-121 B

Sample Name: Aldicarb in DCM

Treatment Level: 0.0060 rng/liter

Fate: _D X E __EM

Path. Paf,e I! 14

Test No.: 14

Clinical Historv

Gross Findine:s

Date Time

Nasal cavity. Trachea. Liver, Kidneys. Bronchi, and fl/I/,/flr:. ll..:;1'\ A"

Lungs - No gross lesions. Oroan "'to ,( c)

Liver

Kidnevs

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads

Bodv WeiQ:ht

I

I

Prosector: \ H}!Sto?d

'i'md: !

D Sponta~eous Death .~' ", ' '; !_~_ 1"':~ ,

Eutnah~~~,d wll~n~.~:M;~.~~:::'
Path. Pace (I 15

Test No.:

Sample No.:

14

48-121 B

Report 48-136

Appendix 2

Test Name: Acute Aerosol Inhalation Toxicity (LC50) _

Species: Rat Animal No.: 85-17174,'-

- Sample Name: Aldicarb in DCM

Treatment Level: 0.0060 mg/liter Sex:

Fate: D XE EM

Kodachrome: Gross

Strain: ZM/s-D

Female Age:

Date of Death:

Micro

12-13 weeks

8/14/85

Clinical Histor'

Gross Findin s

Nasal cavity. Trachea, Liver, Kidneys. Bronchi. and

Lungs - ~o gross lesions.

D =
Report 48-136

Appendix :2

sample Name: Aldicarb in DCM

Treatment Level: 0.0020 m2/}lter Perna1e Age: _-'1....2...;- 1....3'--"'w.... wJi.e".ks,,--_

Da te 0 f Death: -.loL8LI l 3'/"/u8"-51.-.__

·~ticro

Sex:

Test Name; Acute Aerosol Inhalation Toxicity (LC50j

Species: Rat Animal No.: 85-17103

Strain: ZM/S-D

-X...E EM

Kodachrome: Gross

path. Par,e [I 16

rest No.: __....a.:14~_

sample No.; _--=4<.1<8~-;.l.1..2..1J._B _

Fate; __D

Clinical Historv

Gross F::'ndings

Nasal cavitv, Trachea, Liver, Kidneys, Bronchi, and

Lungs - ~o gross lesions:-

Date

Or""'"

Liver

Ti!:}.c.

Q. Ol. A"I

Kidnevs

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads

Bodv Wei~htl

I'rosettor: .... H"Src';-
Repo'rt 48-136

Appendix 2

- 85-171 04 ·r

-. Animal No.:

ZM{S D

Bat

Strain:

Mute Aerosol Inhalatjon Tpxjcity (1'[50)

Species:

14 Test Name:

48-121 B

AJdicarh in DCM

Path.. Par;e (I 17

Test No.:

Sample No.:

Sample Name:

Treatment Level: Sex: __....F"'p;.um""auJ"'p'-- _

Fate: D

Q QQ?Q mgIJ iter

EM

Kodachrome: Gross

Date of Death:

Hicro

Age: 12-13 »'peks

- 8/13185

Clinical Historv ,

'1

Gross Findings

Date

Nasal caVity. Trachea, Liver, Kidneys, Bronchi. and

Lungs - ~o gross lesions.

8/13/85

Orl<!an

Liver

9:09 Al-I

Kidne....·s

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads I

Bod..... t<eil:(hti

D Spontaneous-Death
Report 48-'136

Appendix 2

Path. Par.f..' I' 18

8113/85

Age: 12·-,13 weeks

Date of Death:

Micro

Sex: Female

Gross

Test Name: AcUte Auoso1 inhalation Toxicity (Le50)

Species: Rat :. Animal No.: 85:17105

Strain: _'7ZM......! ... S---'D"-- _

Kodachrome:

Test No. : 14

Sample No.: 48-121 B

Sample Name: Aldicarb in DCM

Treatment Level: 0 0020 mg/liter

Fate: __D J-E EM

Clinical Historv --I

Gross Findings

Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and

Lungs - ~o gross lesions.
-, 85-17106

12-13 weeks

8/13/85

Age:

Animal No.:

ZM/S-D

Rat

Date of Death:

IHcro

Strain:

Female

Species:

Aldicarb in DCM

48-121 B

(I 19

14

Report 48-136

Appendix 2

Sample Name:

Path. PaBe

Test No.:

Sample No.:

Treatment Level: 0.0020 mg/liter Sex: ---------- Fate: D X E EM

Kodachrome: Gross

Clinical Ristor

Gross Findin s

Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and

Lungs - No gross lesions.

Kidnevs

S leen

Heart

Brain

Pituitar

Adrenals

Gonads I

Body Wei ht

,', ",I, ,:,::-

. -~.-~:. ;

,.I,
Sample No.: 48-121 B Species: Rat

SalIIple Name: Aldicarb in DCM St.rain:

Treatment Level: 0.0020 mg/liter Sex: Female

Fate: __D J.....E EM Date of Death:

Kodachrome: Gross !'licro

Pa th. Par,e (/ 20

Test No.: 14 Test Name:

Report 48-136

Appendix 2

R E COR D ------

Acute Aerosol Inhalation Toxicitv (LC50)

Animal No.: 85-17107

ZM/s-D

Age: 12-13 weeks

8/13/85

- I Clinical Ristorv

Gross Findin2s

I Date Til!1e~

Cervical lymph node - Enlarged 3.5X normal. 8/13/85 9: 14 A.'l

Oro:>" \it (c)

Nasal Tt"Bchea, Liver, Kidneys, Bronchi, and Liver I cavity, Kidnevs I

Lungs - No gross lesions. Snleen I

Heart !

Brain I

Pituitary I

Adrenals i

Gonads I

Bod\' Weil!htl

.-,.-

.~..
Report 48-136 " I

Appendix 2

__--<..:14::....._ Test Nanle: Acute Aerosol Inhalation Toxicity (LC50) ......

48-121 B Species: Ra t Animal No.: 85-17098 ."

-

Path. Pace j,2l

Test No. :

Sample No.:

- 9-10 weeks ",.

-.. 8112/85 "~

Sample Name: Aldicarb in DCM Strain: ....Z..... M!..L.I....Se--"'D ..:::."\

Treatment Level: 0.00082 mg/liter Sex: __--IF-'ie<Jjm...a....l....e'--___ Age:

Fate: D.-X..E EM Date of Death:

Kodachrome: Gross Micro --,.,

1- ---=C.=.I.=.i::..:n-=.ic:=;a:=;I::......:}c:,:ll:.:·s::..:t:.:o:.:r:...ly'---

Gross Findings

Right kidney - Marked hydronephrosis.

Date

R/]7/Re;

Oraan

Time :--l

g. 1" A\I .

Nasal cavitv, Trachea, Liver, Left kidney, Bronchi, and

lungs - No gross lesions.

Liver

Kidnevs

Spleen I

Heart

Brain

.Pituitarv I

Adrenals

Gonads I

Bod" ~eight

Prosector:

D Spontaneous Death E ... Euthanized EH Euthanized .:he:l. r..::,db:':::!d'

6/.ii.
Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Ra t Anima 1 No.: 85-17099

14

48-121 B

8/12/85

Report 48-136

Appendi:-: 2

Female

Strain: -=Z::.:.M:.!./~S~-~D ,..._-----

Age: __9:..-_1:..;O::......:w:..::e:..::e~k:.:s:-..-

Date of Death:

Micro

R E COR D ------

0.00082 rug/liter Sex:

~E EM

Kodachrome: Gross

Treatment Level:

Fate: __D

Path. Pace (I 22

Test No. :

Sample No ••:

Sample Name: _-,A2:.:ld~i::c~a,=-,r~b~· ...i!:.-'n~D:.::C::..M:....- _

me

I Clinical Historv

Gross Findin~s

I Date Ti!:!!' R /1., I RO; Q.f\-q A'1

Right kidney - Marked hydronephrosis. Or"",,., "'~ (£' )

Trachea, Liver, Left kidne\', Bronchi, Liver

Nasal cavitv, and Kidnevs I

lungs - No gross lesions. Spleen I

Heart

Brain I I

Pituitarv I

Adrenals i

Gon:lds

Bod\' "'eight !

I

I

i

I

I

I

I

I

I

I

" .,.

I

----------------------~-----_.._---------

D Spontaneous Death E ., Euthanized Euthan'ized ~henmcri~~=:

:0.':.::(: ..··
Acute Aerosol Inhalation Toxicity

"'Vf!"

Report 48-13,?"

Appendix 2 . ~: . ......

.,·:~l\

.. (Le50) , ;~ij:

85-17100'~~

8/12/85 .'tf;

Age:

Animal No.:

ZM/S-D

Female

Date of Death:

Micro

Species: R:.:a=-=..t _

Strain:

Clinical History

Test Name:

0.00082 !ltg/liter Sex:

~E EM

Kodachrome: Gross

14

Aldicarb in DeM

48-121 B

Fate: D

Path. Page 11 23

Test No.:

Sample No.:

Sample Name:

Treatment Level:

...,...

:?l

••• 1

Gross Findings

Kasal cavitv. Trachea. Liver. Kidneys, Bronchi. and

Lungs - No gross lesi~

Date

8/12/85

O"oan

Liver

Time '."1

8: 59 &'1 . .!

Wt (I!)

Kidnevs

Spleen

.1

Heart

Brain

Pituitary

Adrenals

~G~o~n~a~d:'1s;===:=9====="~ Bod...·- I-I'eight I i.

D Spontaneous Death E = Euthanized

ProsectOr: .H}lSr'"el --1

... ' ". m,d<.•j '.

E~I = Euthanized '~1-H~'n~o;ib~/
9-10 WE:E:ks

8/12/85

Age:

ZM/S-D

Female

Strain:

Date of Death:

Micro

Sex:

Gross

Test Name: Acut~ Aerosol Inhalation Toxicity (LeSO)

Species: Rat Animal No.: 85-17101

Report 48-136

Appendix 2

Path. Pace 1./24

Test No.: 14

sample No.: 48-121 B

sample Name: Aldicarb in DCM

Treatment Level: 0.00082 mg/11ter

Fate: __D -LE __EM

Kodachrome:

I Clinica1 Historv

I

- Gross Findings

Date Til:le

8/12/R~ q·o.!, AV

Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and Or2an t-;'t . (r. )

Lungs - No gross lesi~ Liver

Kidnevs

Spleen I

Heart

Brain

Pituitarv

Adrenals I

Gonads I

, Bodv Weight I

-

"

, ,

I ,.,: l

i' Prosector: H~··':::'- - i

, rnd i .,,1

D • 'Spontaneous~Death E "" Euthani'zed
Test Name: Ac~te Aerosol Inhalation Toxicity (Le50)

48-121 B Species: Rat Animal No.: 85-17102

Aldicarb in DCM

,

.i

J.j I

_,f

-.1.; ., ..

:..

--;l:

9-10 Io;eeks....:..~·:

.';'

8/12/85 .•..

Report 48-136

Appendix 2

Age:

Strain: ZM/S-D

Sex: ~F~e:.!!.m~a,-:!:l,",e,,- _

Date of Death:

Gross Micro

PAT H 0 LOG Y

EM

Kodachrome:

0.00082 mg/liter

-XE

14

Treatment Level:

Fate: D

Path. PaGe (I 25

Test No. :

Sample No.:

Sample Name:

" .. .'.\

. . . - D Spontaneous Death E'c Euthanized

Clinical History

Gross Findings

I Date Time__

Trachea, 8/12/85 8~!.1 101 Nasal cavity, Liver, Kidneys, Bronchi, and OrO"" ~1' (01

Lungs - No gross lesions. liver

Kidneys I

'Spleen

Heart

Brilin

Pituitilr...·

Adrenals I

Gonads I

Bodv I.'eight I

i'

Prosector: . H~lSt,~el: .. .crod:·: .,', ':-., ',,' ,',; .. }.:,
Report 48-136

Appendix 2

Path. Par.e (126

Clinical Historv

Sex: __-lMa-.,lue:....-_____ Age: 7-8 weeks

Date of Death: 7..:./_]..;::2~/..:.8~5 _

Gross !oUcro

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-1676"-

Strain: ZM/s-D

Kodachrome:

Sample No.: ~4:il80!.:-:..lw2...1I-'AI..- _

SalIlple .Name: Aldicarb DCM

Treatment Level: 0.0463 m~/liter

Fate: -2..D E EM

1est No.: 1~4:-..__

--I

Gross Findin2s

Eyes - Red encrustation, bilateral.

- Left - Lenticular opacity.

- Right - Corneal opacity.

Date

.,/1?/fl<;

0,.°""

Liver

Time

17· 1 R PV

Right kidney - Moderate hydronephrosis. .Kidnevs I

5nleen I

Lungs - Dark pink. Heart i

Brain

Kasal cavity, Trachea, Liver, Left kidney, and Bronchi -

Ko gross lesions.

Pituitary I

Adrenals i

Gonads i

Bod\' Weight I

E., Euthanized
Test Name: Acute Aerosol Inhalation Toxicity (LCSO)

48-121 A Species: Rat Animal No.: 85-16765

Strain: _...;Z::;M~/:...:S::..-..::.D:...- _

Report 48-136

Appendix 2

R E COR D ------

Sample No. :

SampIe Name: _~A:=l:.d..:i:.c:.a:=r:.b~D..:C::;M=-- _

Path. Pase I' 27

Test No.: 14

Treatment Level: 0.0463 mg/liter Sex: __.:.:Ma=l:.e _ Age: _--:.7_-...:8:......;;w..:e..:e~k:.:s~_

Da te of Death: -=-7:...11:.:2:.:/..::8:.::5~ _

folicro

E EM

Kodachrome: Gross

Fate: ..1L-D

Clinical History

Gross Findings

Eyes - Right - Lenticular opacity.

- Left - Corneal opacity.

Date

Oroan

Time

12:'2 PH

Lungs - Dark pink, all lobes. Liver

Kidnevs

Nasal cavity, Trachea, Liver, Kidneys, and Bronchi - No

gross lesions.

Spleen

Heart

Brain

Pituitary

Adrenals

Gonads

Body WeilZht

D Spontaneous Death .E" Euthanized

:pro~,e~t~~/••:~~l~t,~~,i,.i~~>~i: "~:'" '. :':.>;,-.{::":, I

",,~'\ ~'. ~;~ft~~;,~~:f,~~~t~~r~~
Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-16766

Report 48-136

Appendix 2

Path. Pa~e 1/26

rest No.: 14

Sample No.: _.::4.:::8--.:;.12=..1=-.:A"------

Age: _--'7'--~B::........:w:.:e:.:e:;.:k~s::...._

Strain: ZM/S-D

Sex: __.:.M~a~l:=e,-- _

Date of Death: ----'7"-t/~1:..;;2"-/..!:!8~5 _

~1icro

E _EM

Kodachrome: Gross

Fate: -LD

sample Name: Aldicarb DCM

rreatment Level: 0.0463 mg/liter

Clinical Historv

Gross Findin2s

Eyes - Red stained, bilateral. Date Tim~.

7/12/85 12:05 p~

Or C7 a'" \\It (I') Nasal cavity, Trachea, Live£, Kidneys. Lungs. and Bronchi-r.=L~i~v~e~r~b===~~~=d~==~

No gross lesions. Kidnevs

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads I

Bodv ~ei12htl

D,,= SpantarleOus .Death'
•

Report 48-13&

Appendix 2 R E COR D ------

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-16767

Path. Pace (I 29

Test No.: 14

Samp le No.: _..::4~8:....-...:1..::2...:1--.:.:A _

Sample Name: _..:.A.::l~d::.:i:.:c:.:a::.:r:.:b:......:D:;,;C::.:M..:..... _

Treatment Level: 0.0463 mg/liter

7/l2//j5

l.ge: __7~-...;l:l~w~e::..:(.:,;J,.~·,,~.__

ZM/S-D

Micro

Strain:

Sex: __..:M:a:..:l:;.:e=-- _

Date of Death: _ EM

Kodachrome: Gross

Fate: -LD E

Clinical History

Gross Findings

Date Time

Eyes - Lenticular opacity, red encrustation, bilateral. 7712785

On'an

11:51 AH

Lungs - Mottled light and dark pink, all lobes. Liver

Kidnevs

Nasal cavity, Trachea, Liver, Kidneys, and Bronchi - No

gross lesions.

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads

Bodv Wei!!.ht

,L

I'

I
"

path. Pace It 30

R E COR D ------

Report 48-136

Appendix 2

Test No.: 1..,4=-_

Sample No.: 48-121 A

Test Name: Acute Aerosol InhalatioD Toxicity (LC50)

Species: Rat Animal No.: 85-16768

Male Age: __~7~-~8~'i~e~.~k~5 _

Dateof Death: _7L.ft-l....2ufuBJ..5L- _

Micro

Sample Name: Aldi cub DeM

Treatment Level: 0.0463 mil/liter Sex:

Fate: ..lL..D E _EM

Kodachrome: Gross

Strain: ZM/S-D

Clinical Ristor'

Gross Findin s

- Lenticular opacity, bilateral.

Nasal cavity. Trachea. Liver, Kidneys, Lungs. and Bronchi

Ko gross lesions.
i ,. 1

ht

-or

Sex: __..:.M::;a""l..."eO--_____ Age: __....I6l:.-:....7~wt.lie:J;:e:JSk..:ls'_·'

Da te 0 f Death: 71..J,...{Z'-SJ..{u8:l.5l.-- ,l

Gross Micro

Clinical Histor

Test Name: Acyte Aerosol Inhalation Toxicity CLC50)

Species: Rat Animal No.: 85-17036

Strain: ZM/S-D

0.0087 mg/liter

_E _EM

Kodachrome:

Eyes - Lenticular opacity, red stained, bilateral.

Report 48-136

Appendix 2

Right kidney - Mild hydronephrosis.

Trachea, Liver, Left kidney, and Bronchi - No gross

lesions-.--

Perinasal area - Red stained.

Lungs - Dark pink, all lobes.

Path. Paee (131

Fate: _~...D

Test No.: 1~4;:......_

Sample No.: _-=4!.!:8~-~1-=2~1-,B~ _

Sample Name: _~Al:l~d!.!i,-!:c:..!:a!.!r:.Bb!......!:!D~Cc!:!M,-- _

Treatment Level:

"I

t r============~~========~1 Gross Findin s·-::i
Report 48-136

Appendix 2

Strain: ZM/S-D

Sex: __...:M.=:a:.::l:.,:e;-._____ Age: 6-7 weeks

Da te of Death: --'7'-1./-=2""5..../.:::.8;:.5 _

Micro

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-17037

PAT H 0 LOG Y

-----_._--

E EM

Kodachrome: Gross

Fate: -l.-D

Path. Pace II 32

Test No. : 14

Sample No.: _~4:..:8_-..:..1.::.2::...1.;;;.B _

Sample Name: Aldicarb DeM

Treatment Level: 0.0087 mg/liter

Clinical Ristor·

Gross Findin s

Eyes - Lenticular opacity, red stained.

Perinasal area - Red stained.

Lungs - Dark pink, all lobes.

Trachea, Liver, Kidnevs, and Bronchi - No gross lesions.
Gonads I

Trachea, Liver, Kidneys, and Bronchi - No gross lesions.

R E COR D ------

Clinical Historv

Test Name: Acute Aerosol Inhalation Toxicity (LeSO)

Species: Rat Animal No.: 85-17038

PAT H 0 LOG Y ---------

0.0087 mg/liter

_E _EM

Kodachrome:

Aldicarb PCM

14

t.8-121 B

XD

Perinasal area - Red encrustation.

Lungs - Dark pink all lobes.

- Red encrustation, bilateral.

- Right - Lenticular opacity.

Path. Pace 1133

Sample No. :

Sample Name:

Treatment Level:

Fate:

Test No.:

Bod\' Weight ' '~

Report 48-136 ·W

Appendix 2

Lf

.~

-, !

-f Strain: _--,,~.ao..;..~. ZM/S-D -:." ~.

Sex: --.... :"":"":'"":'-o-f-p-e-a-t-h-:- Age: ~~ 5~:~h v =.'.~ Gross Micro l

i

: ,.

i

·i 1======================~~'=h.================If:.r Gross Findinl'.s t-----------------"-';...;;.c~~;;.;..;.c==:..-------r_-----.,..----_.;.j~.

7~~::5 ~:~\;~:I

Liver ,]:'. I-=.:..;.:::.:...__.-+-----~.:

Kidnevs

Spleen !!!

. . .

,:

I; ......,:

.' c,,'

.D· .. ~~o.h;,meC>u~Death~:.....·· " .>.;:.~'" ~, :.,
Age: __~6=-.L7--:&1.w.l:.e.l:.e.ll.k::..s__

Test Name: Acute Aerosol Inhalation Toxicity (LesO)

Species: Rat Animal No.: 85-17039

Report 48-136

Appendix 2

Strain: _~Z~M!J./...ilS~-M.D _

Treatment Level: 0.0087 mg/liter Sex: __:.:Ma"'""'-l... e _

Fate: l.D E EM Date of Death: -L7L/2....... 'i~/6~).J...· _

Kodachrome: Gross Micro

Path. Pace (134

Test No.: __....:1~4~_

Sample No.: _.....:4~8~-....:l:.o:2:..:.1~B _

Sample Name: _~A=l~d=i~c~ar=-b~D~C::::M:.:... _

Clinical History

Gross 'Findings

Date Time

Cervical lymph nodes - Enlarged 2X normal. 7/25/85 2:30 pv.. OrQan \oil" (0)

Lungs - Dark pink all lobes. Liver

Kidneys - Red stained. Spleen - Left - Lenticular opacity. Heart

Brain

Kidneys - Mild hydronephrosis. Pituitary

Adrenals

};asal cavity, Trachea, Liver, and Bronchi - No gross Gonads

lesions. Body Weight

-

I

..

Prosector: . -H~lS t ~': I

:nd !,

i

. -, '., . '. ..

D = Spontaneous Death _'

,' ~.

E- Euthan:i.zed'
II

Report 48-136

Appendix 2

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No. : 85-17040

Path. PaCe I' 35

Test No.:

Sample No.:

Sample Name:

14

48-121 B

Aldicarb DCM Strain: ZM(S-D

Clinical History

-..: :::

:,

Gross Findings

Date Time -.' ..

~es - Red stained, bilateral. 7/25/8; 2:36 P}j

- Left - Lenticular opacity. On,:an t<.. (~)

Liver

Lun~ - Dark pink, all lobes. .Kidnevs

Spleen

~asal cavitv, Trachea, Live~. Kidneys. and Bronchi - No Heart

gross lesion",. Brain

Pituitary

Adrenals

Gonads ;

Body \-"eight ,

.",

, ,.~

Prosec':tor: .-·}l_\:St ee lX: -'. "

.- "

D ...
Report 48-136

Appendix 2

p", th. Paee [I 36

85-17447

Age: __....;6::..-....;7'--'w::.;E::,;e:,;k'"'s"---_

Animal No.: Rat --='"'-~:....;..:...-

Strain: _....!Z~M:J../...:::S::.-!::.D _

Acute Aerosol Inhalation Toxicity (LC50)

Sex: _--:.Ma=-<l:.l:e~ _

Date of Death: ....!8~/:...J1:.J./..s:8~5 _

Micro

Species:

Gross

Test No.: 14 Test Name:

Sample No.: 48-121 B

Sample Name: Aldicarb DCM

Treatment Level: 0.0060 mg/liter

E EM

Kodachrome:

Fate: ~D

. !,....

Clinical History

= Gross Find1n~s

Date Time

Eyes - Red encrustation, bilateral. s7f785 9:26 A:-l

0,.",,1'\ 1,.,'t (Q)

Perinasal area - Tan stained. Liver

Kidnevs

Lungs - Dark red, all lobes. Snleen

Heart

liver - Mottled light tan and brown. Brain'

Pituitar"

Trachea, Kidneys, and Bronchi - No gross lesions. Adrenals

Gonads

Bod" \o;'ei~ht

I

Prosec tOT·:' H~:St~il ",.

'I t:lC ! .' .,

D Spontaneous..Death E" Euthanized :E!':. lut:ha~~(zed:wh~·ri:,:nCT'ibu~=

·,.····.'.··.,6\1'"f'r·)··•.•··••·
•

Report 48-136

Appendix 2

<

"

6-7 weeks -_....::.....:........:::.::.::.::.:.=-.•. Age:

____......;:;.:-=..:c...::.:: 8/1/85 . ,

Strain: ZM/s-D

R E COR D ------

Sex: __M:..:.a:::.l::.e=- _

Date of Death:

Gross Micro

Test Name: Acute Aerosol Inhalation Toxicity (Le50)

Species: Rat Animal No.: 85-17451

Kodachrome:

Path. Pa8e f.1 37

Test No.: 14

Sample No.: --:4:::.:8~-...:1:.:2:..:1~B"-- _

Sample Name: Aldicarb DeM

Treatment Level: 0.0060 mg/liter

Fa te: -lLD E EM

=-:.'

Clinical Historv ;'

:.' ,.;!

-..

..

-

..

= '.

Gross Findings . i:

:;

Date Time .. . '

Eyes - Red encrustation. lenticular opacity, bilateral. 8/l/R" g'3,} A.'1 ~L~

•....

OroaT'l Wt (\;!) \'~

Lungs - Mottled light and dark red. all lobes. Liver t··

Liver Mottled light and brown. Kidnevs - tan Spleen .. --- f)

Heart :~

l'asal cavity, Trachea, Kidneys, and Bronchi - No gross Brain f~~

.lesio"ns. ---- ? :'

Pituitarv .,

!.l~ Adrenals ('

Gonads

Body Weight I ~:

f.~

..

"

..

i

Prosector: H~lSteel

-'"

.. ',

u_

D = Spontaneous Death E .. Euthanized . E}! Eu thaniied;;henlilor{bu~dJ: ,::" r·;··i:>·i;i77J·~;
8-9 weeks

8(14/85

Species: Rat Animal No. :

Strain: ZM(S-D

Sex: Male Age:

Date of Death:

Gross Micro

Test Name: ~A~c:..!:u!..!:tc!:e~A~e.::.r.!:::.o2.s~o=.l_l=.n!.!;h~a~l~a~t:..:i~o~n~T~o::.:x~i~c.=i.::.t.z.V-.l:.( L~C=:.50~)!..- __

85-17442

Report 48-136

Appendix 2

path. Pace II 38

Test No.: 14

Sample No.: 48-121 B

Sample Name: Aldicarb in DCM

Treatment Level: 0.0060 mg/liter

Fate: __D -LE __:EM

Kodachrome:

Clinical Historv

Gross Findings

Date Time

Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and 8) 14T85 8:39 P" lesi~ oJ

Lungs - tio gross Orp'::tn Wt" (p' "\

Liver

Kidnevs

501een

Heart

Brain

Pitu:harv

Adrenals I

Gnnads I

Bodv Wei£ht

I

I

ProSector: H:-!Stecl. I

rod .. i

I'

D Spontaneous Death . E ., Euthanized .E~l = Euthaniied.whenrncribu:lc

6/77 ~, . >/.;
Report 48-136 t

Appendix 2

Path. Pace f.l 39 !!!.!!Q~QQr !!.f.Q!1!

Test No. : Test Name: .. 14 Acute Aerosol Inhalation Toxici ty (LC50)

Sample No. : 48-121 .B Species: Rat Animal No. : 85-17L.t.8 1

- Sample Name: Aldicarb in DCM Strain: ZM/S-D :.~

Treatment Level: 0.0060 mg/liter Sex: Male Age: 8-9 weeks )

Fate: D -!..E _EM Date of Death: 8/14/85 :.~.

Kodachrome: Gross Micro 1

Clinical History

.;

·f

,

11

Gross Findings n

Da'l:e Ti!!\e i

."

8114/85 8: 33 .A}! I .r • Nasa.l cavity. Trachea, Liver. Kidneys. Bronchi. and OrI!.an Wt (I!.)

Lungs - No gross lesi~ Liver . , l

Kidnevs !

Spleen ,

Heart ' '

"

Brain

Pituitarv ;:

Adrenals "

" ,

Gonads

Body "'eight

.. ~-

:

I . ,

D Spontaneous Death E Euthanized
8-9 weeks

8/14/85

Age:

Strain: ZM/S-D

Date of Dea':h:

!-licro

Sex: Male

Gross

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-17449

Kodachrome:

0.0060 mg/liter

~E EM

14

Rt:port 48-136

Appt:ndiz 2

S&~?le ~o.: __~4~8~-~1~2~1_B~ ___

AId 1° carb l'n DCM s.::;>le Name:

:reatment Level:

:~st ~o.:

D Spontaneous Death E '" .Euthanized

- C1 inical History -

,

......

I

I

:

,

Gross Findin25

; Date Time 0

~3sal cavit)'. Trachea, Liver, Kidneys. Bronchi, and 8/14/85 8:25 A.': I

1 Lungs - No gross lesions. 0,..,,,,,., Wr (" ) I

I

I Liver

I

Kidneys

Spleen

I

Heart

Brain

Pituitary

Adrenals

Gonads

I

Body Weight I

i

I

i

Prosector :, 'H~:S(c",: ,<

, ;.-, ::--,:: !
Report 48-136 J..,

Appendix 2

Age:

ZM/S-D

Inhalation Tox;City (LC50)

Animal No.: 85-J7407

,R E COR D ------

Acute Aerosol

Sex: __"l;MjQau.".l::e _

Species: _-'-,--..QR... a.... t _

Aldicarb DeM Strain:

Sample No.: __~4~8~1~2~J~B~ ___

Sample Name:

Fate: ....L-D

Treatment Level: 0.0020 mg/l;rer

Path. paee Ii 41

Test No.: 14

1 __.J,;6L,:-:.L7......wWl:.el:. ekU5-'q

E EM Date of Death: -'-ZJ,./...13J,.J,L/.c. 8.J.5 t.

Kodachrome: Gross Micro :

1-- F=============::~=;==:=§==================_.:'I , ....::.C=.l=.in::.J.=.·c:::a:::l:....:H~i:.:s::.:t::.:o:.::r:..l-v _l

;)

, :i

Gross Findin~~ __,-----__,------l

Eyes - Red encrustation, bilateral. t===;i;!,Da~tg,e==F==Tb;i~ml1Jie"===I' ,!

7f<1/P,r:.. 10·;') AM

Perinasal arga - Red stained.' Liver

Right kidne: - Mild hydronephrosis.

Kidnevs

Soleen

T~achea, Liver, Left kidney, Bronchi, and Lungs - No

gross lesions.

Heart

Brain

I--=-P.:.i.::,tu::.J.:::.·t::;a:::;rv::..:.._I- -1% :

Adrenals

Gonads

Bodv Wei>tht

D : Spontaneous Death E'" E~thani~~d'
Report 4B-1 3h

Appentli;.; 1

Pa:h. Pa[je II 41

Test No. : 14

Sample No.: 48-121 B

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-17406

sample Name: Aldicarb in DCM

Treatment Level: 0.0010 mg/liter Sex:

Strain: __~Z~M'-L/~S:...-.::;D _

Male Age: _...:B~-_9~w:.:e:..le:..!k~s~__

Fate: __D JLE _EM

Kodachrome: Gross

Date of Death:

Micro

8/13/B5

".'.';.

, -.;< ':'/:,-: ,; ,.,.~:' ',-.",-'.':

Clinical Ristorv

Gross l'indin2s

Cervical lvmph nodes Enlarged 2X normal. Da~" Time - 8/13 185 8:32 ~l

Multiple red foci disseminated Oro,," \-iT (;)

Lungs - over all lobes. Liver

Right kidney Mild hydronephrosis. Kidnevs - Soleen

Nasal Trachea, Liver, Left kidney, and Bronchi Heart cavity, - Br.;-i.n ~o gross lesions. Pituitary

Adrenals

Gonads

Bodv Weieht

I

I

I

I

Preis.ector: H:-!S t:e,,: .,

"-'';::;': , I': ",' '•.•t....:

D";' Spontaneous Death
Path. Pil~e /1 4J

PAT H 0 LOG Y R E COR D --------- ------

Report 48-136

Appendi~: 2

Test No.: __--=1""'4'--_ Test Name: Acute Aerosol Inhalation Toxi~ity (LC50)

Sample No.: __~4~8~-~1~2~I~B __

Sample Name: Aldicarb in DCM

Species: Rat

Strain:

Animal No.: 84-17408

ZM/S-D

Treatment Level:

Fate: D

0.0020 mg/liter

-X-E _EM

Kodachrome:

Sex:

Gross

Male

Date of Death:

!-licro

Age: 8-9 weeks

8113f85

Clinical Historv i

Gross Findings

Right kidney - Marked hydronephrosis.

Nasal cavity, Trachea, Liver, Left kidney, Lungs, and

Bronchi - ~o gross lesions.

Date

8/11/R'i

Liver

Kidne'\'s

Time

Spleen

Heart

Brain

Pituitary

Adrenals

Gonads ,

Body t,Teight '

Pro'sec tor:

", _.!=.•.'<

D'}.;_~~i6ntarie!?,us Death
•

Report 48-136

Appendi:~ 2

Path. P;i[\€ /144

Test No.:

Sat:lple No.:

14

48-121 B

Test Name: Acute Aerosol Inhalation Toxicity (LeSO)

Species: Rat Animal No.: 85-17409

Sample Name: Aldicarb in DeM ZM/S-D

Treatment Level: 0.0020 rna/liter

Strain:

Sex: _-.....JMai:!SL1""elL- _ Age: _----'8~-::.9~w~e=.;e=.;k~sL_

Fate: __D -X....E _EM

Kodachrome: Gross

Date of Death: ~8CL/~1_""3L/~8~5 _

Hicro

.,:';_ - ,j", ••"••••• "/". ',c. . -: :',. :;,.:-,'~_ (;:;;" ..:.~./'.~'::"; • /~:

.. "'i;§~(tb";~~ih~'~:i,i~~S~,~~'f(h~t'~~lt~t\ti;:iil~ . "",' :,", - .',""::'-

!·E",Euth~ti:ized .~."

I Clinical History

f

I - Gross Findings

Date Time

Right kidney - ~a:!.d hydronephrosis. RII1/A:; B: "2 101

Oroan \\,T" .,,,)

:\a5al cavitv. Trachea. Liver, Left kidney. Bronchi. and Liver

Lungs - :\0 gross lesions. Kidnevs I

Suleen

Heart I

Brain

Pituitary I

Adrenals

Gonads

I Bod"\' W'ei12ht -

I

I

I

I

i

I ("

I

I

,

! 0

!

I

I , .,

-, .~~'\' ....... '..
":..

Report 48-136

Appendix 2

Strain:

Date of Death:

Micro

(LC50) .-i

--7r·

85-17410 "*:/ ---;~

ZM/s-D '\ ---.......L.><.-=---------~t~·

Sex: __L<MSila..&.l~e______ Age: 8-9 wee ks -X

Test Name: Acute Aeros~1 Inhalation Toxicity

Species: Rat Animal No.:

....A.-E EM

Kodachrome: Gross

14

48-121 B

D

Sample Name: Aldicarb in DCM

Treatment Level: 0.0020 mi/Jjter

Path. Pae.e (/45

Test No.:

Sample No.:

Fate: ______8/13/85 ;' .............WoI..:.....-

.,

F='=============:~=:==~~=======;;;;;;;;;;==========' Clinical History

Gross Findings

Right kidney - Marked hydronephrosis.

Date

R/l1/R"

Time

R·b" AM

Wt (I")

Nasal cavity, Trachea. Liver. Left kidney. Lungs, and

Bronchi - ~o gross lesions.

Liver

Kidnevs

Spleen

Heart

Brain

~P.:::i.::;t.::;u=i=t~a.:;.n':"'_+- --I"

Adrenals ~==-C::'::-:='---+ --1'"

FG~o~n~ao;.d~s===+i=======1 ' Bodv Weight

.

....

'1 '.

D Spontaneou~ Death
Path. Par,e II 46 R E COR D ------

Report 48-136

Appendix 2

Test Name: Acute Aerosol Inhalation Toxicity (Le50)

8/12/85

Age: 9-10 ~eeks

Animal No.: 85-17240

ZM/S-D

Date of Death:

!Hcro

Sex: __-.::!M!2a.:1.s. e _

Species: __R=a~t _

Strain:

14

48-121 B

Sample Name: Aldicarb in DCM

Treatment Level: 0.00082 mg/liter

Fate: __D ...L-E __EM

Kodachrome: Gross

Test No.:

sample No. :

I Cl inical Ristorv

f---'

Gross Findings

n"te Time

8/1i/85 8: 11 A.'":

Nasal cavity~ Trachea, Liver, Kidneys, Bronchi, and 0,..." a'" l,;'~ (p)

Lungs - !\o gross le:;ions. Liver I

Kidne"s I

Snleen I

Heart I

Brain

Pituitarv

Adrenals.

Gonads

Bod\' ,,"'eh:ht

I

PrOSeCtOr: •H~:st.:S'· I :~., . .i ''"-' '-:,,::;q' ,::.

D Spontaneous' Death E :: Euthariiz~d_. "\-....... ' ',,-·f ',',:
Path. Paee I

J47 R E CDR D ------

Report 48-136

Appendix 2

Treatment Level: 0.00082 mg/liter

Test No.:

Sample No.:

Samp Ie NalI'.e:

Test Name: Acute Aerosol Inhalation Toxicitv (LC50)

48-121 B Species: Rat Animal No.: 85-17242

8/12/85

Age: __9:....---'1:....O'---'w;..,;e:.;e""k:.::s~

Strain: ZM/S-D

Sex: _--=Ma=l::.;e:....- _

Date of Death:

Micro

--!..E EM

Kodachrome: Gross

Aldicarb in DCM

14

Fate: D

..ct l-j

,

EN = Euthanized ",hen moribund E - Euthanized

Clinical Historv I

Gross Find1n~s

I Date Time

8/1218S 7:57 AN

!tight kidney - l".arked hydronephrosis. Or"an Wt (p)

Trachea. kidney, Bronchi, and Liver Nasal cavitv, Liver, Left Kidne"'s I Lungs - Ko gross lesions. Spleen

Heart

Brain

Pituitary

Adrenals

Gonads I

Bod\' h'eighti

Prose-ctcr: H~!St",,:'..d

D - Spontaneous Death
Report 48-136

Appendix 2

P3th. Paf;e I! 48

rest No. :

sample No.:

14

48-121 B

Test Name: Acute Aerosol Inhalation Toxicity (Le50)

Species: Rat Animal No.: 85-1724~

Sample Name: Aldicarb in DCM

Treatment Level: 0.00082 mg/liter Sex:

Fate: __D -LE __EM

Kodachrome: Gross

Strain: ZM/S-D

Male Age: 9-10 weeks

Date of Death: 8/12/85

Micro

~rosector: HX~teel

md

I Cl'l.nl.ca . 1 Historv

Gross Findings

I n"te Time il/l,/.q~ .q: 05 A}~

Kasal cavity, Trachea, Liver, Kidneys, Bronchi. and Oro",,, \<'t (,. )

Lungs - No gross lesions. Liver

Kidneys

Spleen

Heart

Brain

Pituitarv

Adrenals

Gonads I

Bodv Weieht

-,

- - ----- ---._,

D = Spontaneous Death E • Euthanized EM = Euthanized when rnoribu~d

6/ii
R E COR D ------

Clinical Histor

Age:

ZM S-D

Test Name: Acute Aerosol Inhalation Toxicit

Sample No. : I.A-I21 B Species: Rat Animal No.:

Sample Name: Aldicarb in DCM Strain:

Treatment Level: 0.000B2 mg/liter Sex: Male

Fate: D -LE EM Date of Death:

Kodachrome: Gross Nicro

Path. Pace 1/49

Test No.: __~1....;4__

Gross Findin 5

Right eve - Lenticular opacity.

Nasal cavity, Trachea, Liver, Kidnevs, Bronchi, and

Lungs - No gross lesions.

D = Spontaneous Death E • Euthanized E" Euthanized when rncribund

.6/77.-
Path. Par,e (I 50

Report 48-136

Append i :.: 1

Test Name: Acute Aerosol Inhalation Toxicity (LC50)

Species: Rat Animal No.: 85-17251

lest No.: 14

Sample ~o.: _....;4::tJ8....---'l...2.....1'--"5:.- _

Age: ----<9_-.... 1Q~w....,e....e....k...s'-__

S/l2lBS

Strain: ZM($-D

Sex: __...JMaI:lQ.101.Je _

Date of Death: ____-Ju..<...J............... _

Gross 1'1icro

sample Name: Aldicarb in DeM

Treatment Level: 0.00082 mi(Jiter

Fate: D -X-E __EM

Kodachrome:

I Cl'~n i ca1 H'lstory

Gross Findings

I Date Til:le_.

R/l?/ll,<; R'li A\~

Cervical lymph nodes - Enlarged 3.5X normal. Or12an ~t (,,)

liver

Right kidney - Mild hydronephrosis. Kidnevs I

Spleen Kasal cavity, Trachea, ~, Left kidney, Bronchi. and Heart

Lungs - No gross lesions. Brain

Pituitarv

Adrenals

Gonads - Bodv ~eight

I

-

I

I

I

Prosector: ~~Ste~} ;

we

D .. Spontaneous Death E so Eutha11ized Euthanized ~hen L.~"i~u~:

6:';-:
•

BUSHY RUN RESEARCH

R. D. 4, ...lIon Road, Export, Pennaylvanla 15832

CENTER

UCC BUSINESS CONfIDENTIAL: Not to be released

outside UCC without the written consent of

Dr. R. L. Baron, Manager of Toxicology, Union

Carbide Agricultural Products Company.

Project Report 49-65 Revised

7 Pages

May 15, 1986

Aldicarb in DeM (3 Solutions)

Acute Peroral Toxicity Study - D.O.T. Classification

Sponsor: Union Carbide Agricultural Products Company

* * * * *

SUMMARY

A rat peroral toxicity test was completed on each of 3 concentrations of

aldicarb in DCM. The procedure followed the standard BRRC protocol modified to

conform to Department of Transportation (D.O.T.) test conditions. Results from

this study, expressed in terms of each solution as received, are as follows:

Peroral, Rat (Fasted)

1.0~ Concentration

Males: SO mg of solution per kg of body weight (b.w.)

killed 5 of 5.

Females: 50 mg per kg b.w. killed 5 of 5.

Combined: 50 mg per kg b.w. killed 10 of 10.

- Q.5~ Concentration

Males: 50 mg per kg b.w. killed one of 5.

Females: SO Mg per kg b.w. killed 3 of 5.

Combined: ~per kg b.w. killed 4 of 10.

Q.3~ Concentration

Males: SO mg per kg b.w. killed 0 of 5.

Females: .50 mg per kg b.w. killed 0 of 5.

Combined: ~er kg b.w. killed 0 of 10.

Bushy Run Research cenler

A Jolnl Mellon Instltule-Unlon carbide Corporation Operation
Report 49-65 Revised

Page 2

MATERIALS

Three samples of aldicarb in DCM (dichloromethane) were received from

Union Carbide Agricultural Products Company, Research Triangle Park, NC on

February 17, 1986. Each sample, as received by the Bushy Run Research Center,

consisted of 100 ml of a colorless, transparent, non-viscous liqUid. The

containers bore the identification numbers HTS6806AC (1.0% Active ingredient),

HTS6806AD (O.5~ active ingredient) and HTS6806AE (O.3X active ingredient).

BRRe Sample Numbers 49-45, 49-43 and 49-44 were ~ssigned to the respective

materials. The BRRe Project Humber for work on these samples is 86-03-10722.

The samples were dilutad in corn oil (Mazola). The dilutions were mixed on a

magnetic stirrer for approximately 5 to 20 minutes before dosing and during

dosing.

~tmental Animals

Male and female Sprague-Dawley albino rats, weighing bp.tween 200 and 250 g,

were used because of this laboratory's past experience with this species and

strain. The rats vere obtained from Harlan Sprague-Dawley, Inc.

(Indianapolis, IN) and were acclimatized for at least 5 days before they were

dosed. Upon receipt, they were housed in Room 109, where they were

subsequently dosed and observed until sacrifice. All rats were assigned

unique animal numbers and were identified by toe clipping.

The rats were housed in cages, 5 per cage. with wire floors under which

deotized animal cage board was placed. They were maintained on Ralston-Purina

certified rodent chow and water provided by an auto~atic water system. Water

was supplied by the Municipal Authority of Westmoreland County (Greensburg,

PA) and was available at all times except during the actual dosing period.

TEST PROCEDURE

An appropriate amount of suspension was administered by stomach intubation

through a commercial 16 gauge (3 in.) ball-end stainless steel needle attached

to a disposable syringe. The exact amount of sample dosed for each rat was

recorded on the dosing sheet.

Five male and 5 female rats were dosed at 50 mg/kg b.w•• the Department of

Transportation (D.O.T.) Class B poison level. The rats were fasted overnight

(approximatel~ 18 hours) before dosing. Dosed rats were observed frequently

for signs of toxic effect on the first day of the test and twice a day (except

on weekends or holidays) thereafter. Weights were recorded on the day of

dosing and at 7 and 14 days after dosing. After 14 days, all survivors were

sacrificed and necropsied. The above procedure was performed for each of the

3 concentrations of aldicarb in DCM.
Report 49-65 Revised

Page 3

RESULTS

Results from the peroral study are given in Tables 1, 2 and 3 for the

1.O~, 0.5~ and 0.3~ concentrations, respectively. AIlS male and 5 female

rats died following peroral doses of 50 mg/kg b.w. of the 1.O~ aldicarb in

DeM. Signs of toxicity included sluggishness, tremors, salivation,

lacrimation and red tearing. Deaths occurred at 15 minutes to 1.5 hours. At

necropsy, lur~s were dark red and periocular fur had a red crust.

The 0.5% concentration killed one of 5 male rats and 3 of 5 females.

Tremors, salivation, red tearing, kyphosis, piloerection, diarrhea and red to

brown discharge around the nose and eyes ~ere observed. Time to death ranged

from one hour to one day. Survivors recovered at 4 to 5 days. Gross

pathologic lesions included pink lungs, red periocular fur and one cecum with

watery fecal material. These conditions were apparent in the victims. Except

for one female with enlarged, fluid-filled uterine horns, there were no

remarkable gross findings among the survivors.

Intubation of the 0.3~ aldicarb in DCM

produce death in any of 5 male or 5 female

and perinasal fur and diarrhea were noted.

There was no remarkable gross pathology in

enlarged, fluid-filled uterine horns.

solution at 50 mg/kg did not

rats. Red crust on the periocular

All animals recovered by one day.

the males. One female had

REASON FOR REPORT REVISION

The original report on a1dicarb in DCM, dated April 23, 1986, has been

revised. The only changes that have been made are editorial in nature. There

have been no changes in the results originally report.
Report 49-65 Revised

Page 4

Reviewed and Approved by:

S-IS-'i

Date

II /2 4tl~-:!v/v.--"';-:15--J6

Ronald S. Sleslnski, Ph.D. Date

Assistant Director

Acknowledgements:

Single Peroral Test

Manager. Pathology Department

Fred R. Frank, Ph.D.

Director

Susan M. Christopher, B.S.

Research Technologist

Dana L. Cline, B.S.

Laboratory Technician

Edward H. FOWler. Ph.D., DVM

Associate Director

Date

Animal Care

Archives

WPC/esk/0628P-l

05-09-86

Michael J. Cardella, AALAS Cert. III

Supervisor I

Edgar C. Rimler, B.S., AALAS Cert. I

Technologist
rab le ),

Peroral Intubation. Single Dese to Rats

Material: Aldicarb in DCM - 1.0% Concentration Sample No.: 49-45

Dosage, .Deadl

mg/kg Dosed

Days to

Death

Weight Chang~

7 Days 14 Days Si&n~_~Toxi~ltY

Male Rats

Gross P

50

50

5/5 0,0,0,0,0

SIS 0,0,0,0,0

Tremors, salivation, lacrimation,

red tearing in 5 at 12 min. Death

at 15 min to 1.5 hr.

Female Rats

Sluggishness, tremors, salivation in

5 at 10 min; kyphosis, piloerection

in 1 at 20 min; red tearing in 5 at

death. Death at 16 to 33 min.

Lungs dark red; r

tion on periocul

Lungs dark red; r

on periocular fu

LDSO's:

Males: <

Females:

Combined:

50 mg/kg; sample dosed as a 50% (w/v) dilution in corn oil.

< 50 mg/kg; sample dosed as a 50% (w/v) dilution in corn oil.

< 50 mg/kg; sample dosed at a 50% (w/v) dilution in corn oil.

WPC/esk/0628P-l05-09-86
Table 2

Peroral Intubation. Sinfi'e Dose to Rats

Material: Aldicarb in DCM - 0.5% Concentration Sample No.: 49-43

Dosage,

!!!.&LK&

Deadl

Dosed

Days to

Death

Wei~hLChan~e.

7 Days 14 Days Sbns_ oLToxicity

Male_Rats

Gross Pa

50

50

1/5

3/5

1

0,0,0

12 to 45

(mean=32)

33 and 59

(mean=36)

64 to 79

(mean=72)

32 and 51

(mean=42)

Tremors, salivation in 5 at 30 min;

kyphosis, piloerection, diarrhea,

red crust on periocular and perinasal fur at 1.75 hr. Survivors

recovered at 5 days.

Female Rats

Tremors. salivation in 5 at 20 min;

red tearing in 1 at death. Death of

3 at 1 to 1.5 hr. Brown crust on

periocular fur of 2 at 4.5 hr.

Survivors recovered at 4 days.

In victim, lungs d

cecum filled with 

material; eyes wit

tation. In surviv

remarkable.

In victims. lungs 

periocular fur stai

survivors, uterine 

enlarged. filled w

fluid.

LD50's:

Males: )

Females:

Combined:

50 mg/kg; sample dosed as a 50% (w/v) dilution in corn oil.

( 50 mg/kg; sample dosed as a 50% (w/v) dilution in corn oil.

) 50 mg/kg (4/10 dead); sample dosed as a SOX (w/v) dilution in corn oil.

WPC/esk/0628P-l

05-09-86 
TabJ..Ll

Peroral Intubation. Single Dose to Rats

Materic.l: Aldicarbin_DCM - 0.3;1; Concentration Sample No.: 49-44 

Dosage,

!!!.&L1sL

Deadl

Dosed

Days to

Death

WeiAht ChanA~.-R

7 Days 14 Days SiRns of Toxicity

Male Rats

Gross P

50 0/5 38 to 57

(mean=46)

51 to 87

(mean=70)

Red cruat on periocular and perinaeal Nothing remarkab

fur, diarrhea in 5 at 2.5 h~. Recovery at 1 day.

Female Rats

50 0/5 19 and 23

(mean=22)

25 and 31

(mean=28)

Red crust on periocular and perinasa1

fur of 5 at 3.25 hr. Recovery at

1 day.

Uterine horns of 

filled with clear 

LD50's:

Males: )

Females:

Combined:

50 mg/kgj sample dosed as a sox (w/v) dilution in corn oil.

) 50 mg/kgj sample dosed as a SOX (w/v) dilution in corn oil.

) 50 mg/kg; sample dosed as a SOX (w/v) dilution in corn oil.

WPC/esk/0628P-l

05-09-86
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and complete reprodul::tions of the records of U.S'. Environmental Protection Agency

doc:ume~ts u delivered In thll regular course ofbusinesB for mlcro~.

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