UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON, D.C. 20460 

Ms. Stephanie Burton 

U.S. Regulatory Manager, Corn 

Dow AgroSciences LLC 

9330 Zionsville Road 

Indianapolis, IN 46268-1054 

DEC 1 8 2014 

OFFICE OF CHEMICAL SAFETY 

AND POLLUTION PREVENTION 

Re: Corn plant-incorporated protectants (PIPs) with MON 87411 (DvSnt7 dsRNA and Bt Cry3Bb1 

protein), MON 89034 (Bt CrylA.105 and Cry2Ab2 proteins), MON 88017 (Bt Cry3Bb1 protein), 

TC1507 (Bt Cry1F protein), and/or DAS-59122-7 (Bt Cry34Ab1 and Cry35Abl proteins) 

EPA Experimental Use Permit No.: 62719-EUP-66 

Decision No.: 491621 

Application Dated: May 27, 20 14 

Effective Dates: Immediately through March 31, 2016 (includes planting and associated activities, 

e.g., collection of field data, harvesting, and processing) 

Quantity Authorized: 19.63 pounds (8,905 grams) of active ingredient (1.139, 0.3416, 1.961 x 

10-6, 1.309, 14.07, 0.3443, and 2.428 pounds of Bt Cry1A.105 protein, Bt Cry2Ab2 

protein, DvSnt7 dsRNA, Bt Cry1F protein, Bt Cry34Ab1 protein, Bt Cry35Abl protein, 

and Bt Cry3Bb 1 protein, respectively) in 1,113,853 pounds of seed 

Acres: 9,038 acres, including PIP (unregistered= 2,454; registered = 3,907; total= 6,361), nonPIP (1,061), and border (1,616) acreage 

Dear Ms. Burton: 

On the basis of the information furnished by you, the U.S. Environmental Protection Agency (EPA) 

hereby issues the subject Experimental Use Permit (EUP) under section 5 of the Federal Insecticide, 

Fungicide, and Rodenticide Act (FIFRA) to test MON 87411 (DvSnt7 dsRNA and Bacillus 

thuringiensis (Bt) Cry3Bbl protein) alone or in combination with the following plant-incorporated 

protectants (PIPs): MON 89034 (Bt Cry1A.105 and Cry2Ab2 proteins), MON 88017 (Bt Cry3Bbl 

protein), TC1507 (Bt Cry IF protein), and/or DAS-59122-7 (Bt Cry34Abl and Cry35Ab1 proteins). This 

EUP is authorized only in the Commonwealth of Puerto Rico (PR) and in the States of Arkansas (AR), 

Hawaii (HI), Illinois (IL), Indiana (IN), Iowa (IA), Kansas (KS), Minnesota (MN), Missouri (MO), 

Nebraska (NE), North Dakota (ND), Ohio (OH), Pennsylvania (PA), South Dakota (SD), Texas (TX), 

and Wisconsin (WI) for inbred and hybrid development, nursery observations, and testing and collection 

of product characterization data. 
EPA EUP No. 62719-EUP-66 

Decision No. 491621 

Shipment and/or use under this permit are subject to the provisions of 40 CFR Part 172. The Bt proteins 

to be used in this EUP include CrylA.l05, Cry2Ab2, CrylF, Cry3Bbl, Cry34Abl, and Cry35Abl. The 

EPA previously determined the environmental and human health safety of these proteins and exempted 

them from the requirement of a tolerance as follows: 40 CFR §§ 174.502(a) (Bt Cry1A.105 protein), 

174.506 (Bt Cry34Abl and Cry35Ab1 proteins), 1:74.518 (Bt Cry3Bb1 protein), 174.519 (Bt Cry2Ab2 

protein), and 174.520 (Bt Cry 1 F protein). The EPA has also established a permanent tolerance 

exemption for nucleic acids; this includes DvSnf7 dsRNA that is expressed in MON 87411 (40 CFR § 

174.507). The marker proteins phosphinothricin acetyltransferase (PAT), CP4 enolpyruvylshikimate-3-

phosphate synthase (CP4 EPSPS), and phosphomannose isomerase (PMI) are also exempt from the 

requirement of a tolerance at 40 CFR §§ 174.522, 174.523, and 174.527, respectively. Harvested plant 

material from PIPs that have not yet been commercially registered must not be allowed to enter 

the food or feed supply. 

Prior to shipment and/or use of the subject com, you must consult with the pesticide regulatory officials 

and, if applicable, the biotechnology regulatory officials of the states1 in which you will conduct your 

experimental program, and obtain a state permit or license if such is required. Issuance of this Federal 

permit does not negate the need for permission from individual states. Prior to initiating this 

experimental program in any state, you are required to notify the lead agency of the states in which you 

will conduct your experimental program of the specific testing program (when, where, how much, etc.). 

Failure to do so may result in revocation or modification of this EUP. You must comply with all 

requirements of the U.S. Department of Agriculture's Animal and Plant Health Inspection Service as 

they apply to the proposed testing. 

You must provide notice to the state pesticide regulatory agency at least 72 hours before you plant the 

experimental use PIPs. You must also provide a copy of this authorization letter, the stamped, accepted 

EUP label, and a non-confidential portion of the Section G Experimental Program to all cooperators, 

participants, and users prior to initial use of the experimental use PIPs in accordance with this EUP. The 

EPA will notify the relevant EPA Regions of the approval of this EUP by sending copies of this 

authorization letter to them. 

Conditions under which the EPA is issuing this EUP are as follows: 

1. The acres, amount of pesticide, and amount of seed to be used shall not exceed the quantity 

authorized by this EUP. See Tables 1, 2, 3, 4, and~ for specific details. 

T a bl e . I S ummaryo fth e A crea2e among on- ' N PIP B d or er, an dPIP A cres 

State Unregistered Registered Total I Overall State 

I PIP PIP I PIP Non-PIP Border Total 

AR 12 24 36 10 2 48 

HI 172 329 501 58 59 618 

IL 206 330 536 98 178 812 

IN 263 335 598 108 198 904 

lA 268 335 603 103 188 894 

KS 22 38 60 18 18 96 

1 For the purpose of this Jetter, the terms "state," "states," and "state-by-state" includes the Commonwealth of Puerto Rico. 

2 
EPA EUP No. 62719-EUP-66 

Decision No. 491621 

State Unregistered 

PIP 

MN 211 

MO 19 

NE 212 

ND 143 

OH 143 

PA 12 

PR 210 

SD 196 

TX 169 

WI 196 

TOTAL: 2,454 

Registered l Total 

PIP PIP 

328 539 

33 52 

332 544 

269 412 

269 412 

24 36 

385 595 

307 503 

262 43 1 

307 503 

3,907 6,361 

T ba e . l 2 Acrea2e D .. b . b 1stn uhon etween 

I PI P2 

Non-PIP 

98 

18 

83 

68 

68 

10 

80 

78 

80 

83 

1,061 

E xpenmenta 

State 

Registered Unregistered Total Registered Unregister ed Total 

PI P2 

AR 0 0 0 24 

HI 124 60 184 205 

IL 165 90 255 165 

IN 165 90 255 170 

lA 165 90 255 170 

KS 0 0 0 38 

MN 165 90 255 163 

MO 0 0 0 33 

NE 165 90 255 167 

ND 165 90 255 104 

OH 165 90 255 104 

PA 0 0 0 24 

PR 165 90 255 220 

SD 165 90 255 142 

TX 165 90 255 97 

WI 165 90 255 142 

Total: 1,939 1,050 2,989 1,968 

Notes: P I = Protocol 1 (breedmg and associated data) 

P2 = Protocol 2 (characterization) 

12 36 

112 317 

116 281 

173 343 

178 348 

22 60 

121 284 

19 52 

122 289 

53 157 

53 157 

12 36 

120 340 

106 248 

79 176 

106 248 

1,404 3,372 

3 

Overall State 

Border Total 

178 815 

18 88 

118 745 

93 573 

93 573 

2 48 

100 775 

108 689 

145 656 

118 704 

1,616 9,038 

I P rotoco s 

TOTAL (Pl+P2) 

Registered Unregistered Total 

PI+P2 

24 12 36 

329 172 501 

330 206 536 

335 263 598 

335 268 603 

38 22 60 

328 211 539 

33 19 52 

332 212 544 

269 143 412 

269 143 412 

24 12 36 

385 210 595 

307 196 503 

262 169 431 

307 196 503 

3,907 2,454 6,361 
EPA EUP No. 62719-EUP-66 

Decision No. 491621 ~-- -- - - -- ------- --

r r 

. Registered P~ I -, I I I 

.... 

i STATE ":- ~ .... :g I ! z 0 -0 a .... 

.. .. ~ C§ ~ .... ~ ~ I I -I - .. .... I ~ :g 81 ~ 00 z z - ~ u z z 0 0 (.J 0 0 a a .... 0 .... a a 

AR 2 2 2 2 2 2 4 

HI 26 26 26 26 27 27 59 

IL 27 23 26 35 32 32 31 

IN 26 23 26 35 32 32 34 

lA 26 23 26 35 32 32 34 

KS 2 2 2 4 4 4 4 

MN 27 23 26 33 32 32 31 

MO 2 2 2 5 3 3 4 

NE 28 23 27 35 32 32 31 

ND 23 23 23 25 24 24 31 

OH 23 23 23 25 24 24 31 

PA 2 2 2 2 2 2 4 

PR 35 35 35 35 35 35 35 

SD 27 23 26 30 29 29 31 

TX 25 21 24 25 24 24 27 

WI 27 23 26· 30 29 29 31 

Total: 328 297 322 382 363 363 422 

Notes: PI = Protocol I (breeding and associated data) 

P2 = Protocol 2 (characterization) 

":- I .... :g ~ ~ 81 -rh 

.. ~ "' .. 0 ........ .... 

1

1a C> i .. ~ .... :g z z81 

0 z 0 ou, 0 • 2 .... ac .. .. ,.o 

...... ~ ~· ~ ~ .. 

00 ~ ~~ I I! 

z Zc/) z zo 0 oc 0 Ola 20 a a>< 

2 2 2 2 

31 27 27 27 

29 32 31 32 

32 32 31 32 

32 32 31 32 

4 4 4 4 

29 32 31 32 

3 3 3 3 

29 32 31 32 

24 24 24 24 

24 24 24 24 

2 2 2 2 

35 35 35 35 

26 29 28 29 

21 24 23 24 

26 29 28 29 

349 363 355 363 

-- - - - - -- -- - - --- --

i Unregistered PIPs --- ·r-~ ":- .. I .... 

I :g ~ .... 

( - - :g (.J 81 -(.J .... rh .... 

":- .. "' .. 

~ - 0 - -- 

; ;:! .. ;:! .... 00 .... 

:g ~ 00 z z 0 0 0 0 

• .... .... a 

.. .. .. .. ...... 

I - - - ~ .... ~· ~ 

-;:! ~ ~ I ~ ~~ 

' 0 

00 00 00 00 z z z z z zu, Iii 

;::) 0 i 0 0 0 oc ::::» 

II) a a a ::E ao II) 

24 2 2 2 2 2 2 12 

329 26 26 39 27 27 27 17

330 33 25 33 31 31 53 20

335 40 26 65 35 32 65 263 

335 45 26 65 35 32 65 268 

38 4 2 4 4 4 4 22 

328 38 25 33 31 31 53 211 

33 5 2 3 3 3 3 19 

332 39 25 33 31 31 53 212 

269 24 23 24 24 24 24 143 

269 24 23 24 24 24 24 143 

24 2 2 2 2 2 2 12 

385 35 35 35 35 35 35 210 

307 35 25 30 28 28 50 i96 

262 30 23 25 23 23 45 169 

307 35 25 30 28 28 50 196 

3,907 417 315 447 363 357 555 2,45

4 
EPA EUP No. 62719-EUP-66 

Decision No. 491621 

T able 4. Total Pounds of Seed Expected to be Used for Each Protein (for PI + P2 Acres Combine 

AR 

HI 

IL 

IN 

lA 

KS 

1. 

State CrylA.lOS Cry2Ab2 DvSnf7 CrytF Cry34Abl Cry35Abl Cry3bl 

AR 940 940 564 1,034 752 752 1,034 

HI 13,066 13,066 8,084 14,288 10,669 10,669 14,570 

IL 14,006 14,006 9,682 15,087 13,113 13, 113 16,638 

IN 14,993 14,993 12,361 16,215 15,228 15,228 19,458 

lA 14,993 14,993 12,596 16,215 15,228 15,228 19,693 

KS 1,598 1,598 1,034 1,692 1,504 1,504 1;880 

MN 14,006 14,006 9,917 15,087 13,019 13,019 16,873 

MO 1,269 1,269 893 1,363 1,222 1,222 1,551 

NE 14,053 14,053 9,964 15, 134 13, 113 13, 11 3 16,920 

ND 10,434 10,434 6,72 1 11,515 9,071 9,07 1 12,3 14 

OH 10,434 10,434 6,721 11 ,515 9,071 9,07 1 12,3 14 

PA 940 940 564 1,034 752 752 1,034 

PR 14,805 14,805 9,870 16,450 13,160 13,160 18,095 

SD 13,0 19 13,019 9,212 14, 100 11,891 ll,891 15,604 

TX 11,092 11,092 7,943 12,079 10,0 II 10,011 13,301 

WI 13,019 13,019 9,212 14, 100 11 ,891 11,89 1 15,604 

Total: 162,667 162,667 115,338 176,908 149,695 149,695 196,883 

Notes: PI =Protocol I (breedmg and assoc1ated data) 

P2 = Protocol 2 (characterization) 

CryiA.lOS Cry2Ab2 DvSnf7 CryiF 

Ibs: 

grams: 

lbs: 

grams: 

1bs: 

grams: 

lbs: 

grams: 

lbs: 

grams: 

lbs: 

grams: 

5 

Pounds and Gra 

Cry34Abt Cry35Abl Cry3bt 

0.07069 0.01344 

32.06 6.097 

d) 
EPA EUP No. 62719-EUP-66 

Decision No. 491621 

CrylA.lOS DvSnfi Cry1F Cry34Abl Cry35Abl Cry3bl 

MN lbs: 0.09804 0.02941 1.686 x 10·7 0.1116 

MO lbs: 

NE lbs: 

grams: 

ND lbs: 

OH Jbs: 

PA lbs: 

PR lbs: 

SD lbs: 

TX lbs: 

WI lbs: 

TOTAL lbs: l.l39 0.3416 1.961 X 10·

6 1.309 

grams: 516.5 154.9 8.894 X 1()·4 593.8 

Notes: PI =Protocol I (breeding and associated data) 

P2 = Protocol2 (characterization) 

1.224 0.02994 0.2076 

14.07 0.3443 2.428 

6,383 156.2 l,/01 

2. You will immediately notify the EPA of any findings from the experimental uses that have a 

bearing on safety (i.e., the EPA requires reporting of any adverse effects from the use of or 

exposure to pesticides). You will also keep records of production, distribution, and performance, 

and make the records available on request to any authorized officer or employee of the EPA. 

3. You must provide a final report to the EPA within 180 days of the expiration of the permit. The 

fmal report must include all of the items set forth in 40 CFR § 172.8(b)(2) and report on the 

following information: 

• A state-by-state breakdown of actual acreage per protocol; 

6 
EPA EUP No. 62719-EUP-66 

Decision No. 491621 

• A state-by-state breakdown of actual locations per protocol; 

• Any changes to the stated program, including approved changes to study protocols; 

• A list of specific counties where studies took place. 

4. Per 40 CFR § 172.4(b )( 1 )( v ), you must provide to the EPA the name and street address of all 

cooperators as soon as available and prior to planting. You must record any changes to this 

information in an updated report and submit that report to the EPA prior to planting. 

5. The label submitted in connection with this application is acceptable and must accompany all 

shipments of seed under this EUP subsequent to receiving this approval. Labels must also be in 

the possession of the user at the time of planting. A stamped, accepted copy of the label is 

enclosed for your records. 

6. This EUP is not a limited seed increase registration under section 3 of FIFRA, and plantings 

solely for commercial seed increase are prohibited. 

7. The PIP test plots under this permit must not be within Y-t mile of endangered or threatened 

species in the Lepidoptera and Coleoptera insect orders (as listed by the U.S. Fish and Wildlife 

Service) or their Critical Habitats. You must provide the EPA with locations of test plots and an 

analysis of proximity of the test plots to endangered or threatened Lepidoptera and Coleoptera 

species and their designated Critical Habitats prior to planting. 

Should you have questions or concerns regarding the issuance of this permit, please contact Ms. 

Jeannine Kausch of the Biopesticides and Pollution Prevention Division by email 

(kauschjeannine@epa.gov) or by phone (703-347-8920). 

irector 

Office of Pesticide Programs 

Enclosures 

cc: Adrian Enache and Tracy Truesdale, EPA Region 2 (Puerto Rico) 

Fatima El-Abdaoui, EPA Region 3 (Pennsylvania) 

Terence Bonace and Seth Dibblee, EPA Region 5 (Illinois, Indiana, Minnesota, Ohio, and Wisconsin) 

Eugene Thilsted, EPA Region 6 (Arkansas and Texas) 

Jamie Green, EPA Region 7 (Iowa, Kansas, Missouri, and Nebraska) 

Linda Himmelbauer, EPA Region 8 (North Dakota and South Dakota) 

Patti TenBrook, EPA Region 9 (Hawaii) 

7