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### LETTER FROM UNION CAR8IDE CORP TO USEPA SUBMITTING ENCLOSED INFORMATION**Q~** ALICRRB 

### SOLUTIONS IN DICHL.l]f i OJ1E;H;il·:E**AE;~:030L H~HALAHO~I**  (lCUTE TOXICITY STUDIrs W-ATTACH



### ALDICARB SOLUTIONS IN DICHLOROMETHANEB{e)



### (COMPLIANCE AUDIT 

### PROGRAM)


# TSCA

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### calC scott Sherlock·



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### RETURNRECEIPTREQUESTED 38 OLD FlIOGEBURY

### R~Ic:L)(N~(iRYAftff!3lj1r-0001 

## JFIIQ- O,fJC;;l-7C)t:7;C

August21, 1992 UNION CARBIDE CORPORATION



### DocumentProcessingCenter(TS-790) RoomL-100 OfficeofToxicSubstances U.S. EnvironmentalProtectionAgency 401 M Street, SW Washm.gton, DC20460 8892888&&38



### Attn: Section 8(e) Coordinator(CAPAgreement) Re: CAPAgreementIdentificationNo. 8ECAP-Oll0 DearSirorMadam: UnionCarbide Corporation("UnionCarbide")herewith submitsthefollowingreports pursuantto theterms ofthe TSCA §8(e) ComplianceAuditProgram andUnion Carbide's

### CAP AgreementdatedAugust 14, 1991 (8ECAP-Oll0). Thesereports describeaerosolinhalationand acute toxicity studieswithAldicarb solutions 

### in dichloromethane (DCM). The CASRNfor Aldicarb (TEMIK®)is 116-06-3. (1) "Aldicarb Solution (inDCM)LCso

### AerosolAcuteInhalationToxicityTest", BushyRun ResearchCenter, ProjectReport48-136, December 11, 1985. (2) "AldicarbinDCM(3 Solutions): AcutePeroralToxicity Study

- D.O.T. Classification", BushyRunResearch Center,ProjectReport49-65 Revised, May 15, 1986.**Complet~ ~"Ummaries**  

### ofthesereportsareattached. PreviousTSCASection See)or "FYI" Submission(s)relatedto this substance are: (None) PreviousPMNsubmissionsrelatedtothis substance are: (None)

49-65

### This informationis submittedinlightofEPA's currentguidance. UnionCarbidedoes notnecessarily agreethatthisinformationreasonably supportstheconclusionthatthe subject chemicalpresents asubstantialriskofinjurytohealthortheenvironment. In

### the attached reportstheterm "CONFIDENTIAL" may appear. Thisprecautionary statementwasforinternaluseatthetimeofissuanceofthesereports. Confidentialityishereby waivedforpurposes oftheneedsoftheAgencyinassessinghealthand safetyinformation. The Agencyis advised, however, thatthepublicationrights to thecontainedinformationarethe property ofUnion Carbide. Yours truly,

# W~D. AssociateDirector ProductSafety (203/794-5230) WCKlcr Attachment (3 copies ofcoverletter, summaries, andrepor.is)

49-65

# SmwARy
BUSHY RUN RESEARCH CENTER R. D.•, Mellon Roall, Export, Pennsylvania 15632 Telephone 1412) 733·5200 

### Project**~eport** 

### 48-136

Aldicarb Solution (in DCH) le50 Aerosol Acute Inhalation Toxicity Test Sponsor: Union Carbide Agrucultural Products Company



## * * * * 

## * ABSTRACT Five groups of five male and five female Sprague-Dawley albino rats were exposed once for four hours to an aerosol of aldicarb solution in dlchloromethane 

### (DeM). The concentration of the individual components of the test material (aldicarb, dichloromethane and methyl isocyanate) in the chamber atmosphere were determined by chromatographic methods. The mean aldicarb concentrations for the five exposures were 0.0463, 0.0087, 0.0060, 0.0020, and 0.00082 mg/l. The mean dlchloromethane (DCM) concentrations for the same exposures were 0.166, 0.J25, 0.183, 0.094 and 0.043 mg/l. No methyl isocyante was detected in the chamber atmosphere. The mass median aerodynamic diameter for the aerosol particle size distributions ranged from 2.0 to 3.8 micrometers and the geometric standard deviations ranged from 2.5 to 5.0. Mortalities were seen in all but the 0.00082 mg/l male exposure group, and the 0.0020 and 0.00082 mg/L female exposure groups. Ataxia and tremors were seen i 11 concentration groups during exposure. Addit ona c n cal signs observed in some groups on the day of exposure or during the postexposure period 'included periocular, perinasal, and perioral wetness and encrustation, urogenital wetness, lacrimation, exophthalmos, discolored and unkempt fur, an increased or decreased respiration rate, abdominal breathing,**d~creased** 

### motor activity, and a"slow righting reflex. No clinical signs were observed in survivors during the second week of the postexposure period. A loss of body weight or depressed body weight gains were observed in some animals on postexposure day 7. However, body weight gains were observed for all survivors on postexposure day 14. A discoloration of the lungs, perinasal encrustation and staining, and eye abnormalities were observed at necropsy in some animals which died. The lC50 (with 95% confidence limits) for the combined sexes was 0.0039 (0.0028 to 0.0055) mg/L aldlcarb.

### SUltWARY 

# BUSHY RUN RESEARCH CENTER R. D. 4, Mellon Roed, Export, Pennilivanle 11832 Telephone (4121 733-5200


### Project Report 49-65 Revised

Aldiearb in PCM (3 Solutions> Acute Peroral Toxicity Study

- P.O,T. Classification Sponsor: Union Carbide Agricultural Products Company

SUMMARY A 

### rat peroral toxicity test was completed on each of 3 

### concentrations of aldicarb in DCM. The procedure followed the standard BRRC protocol modified to conform to Department of Transportation (D.O,T.) test conditions. Results from this study, expressed in terms of each solution as received, are as follows: Peroral, Rat (Fasted) 1,0% Concentration Males: 50 mg of solution per kg of body weight (b,w.) killed 5 

### of S. 

### Females: 50 mg per kg b,w, killed 5 

### of 5. Combined: 50 mg per kg b.w. killed 10 of 10. 

# - 0,5% Concentration Males: 50 mg per kg b.w. killed one of 5. 

### Females: 50 mg per kg b.w. killed 3 

### of S. 

### Combined: mt..per kg b.w. killed 4 

### of 10. 0,3% 

### Concentration Males: 50 mg per kg b.w, killed 0 of 5. Females: 50 mg per kg b.w. killed 0 of 5. 

### Combined:**~er** 

### kg b.w. killed 0 of 10.
RESULTS



# .

-...-.**-~.:...** . 

### Results from the peroral study are given in Tables 1, 2 and 3 for the 1.0%, 0.5% and 0.3%concentratlons, respectively. All 5 male and 5 female rats died following peroral doses of 50 mg/kgb.w.of the 1.0% aldicarb in DCM. Signs of toxicity included sluggishness, tremors, salivation, lacrlmation.and red tearing. Deaths·occurred at 15 minutes.to 1.5 hours. necropSY, lungs wer.e dark red and periocular fur'h

__d a red crust•.R. O. ". Mellon ROlRd, Export.**~.nnlyIY.nl.** 15632 

# BUSHY RUN RESEARCH CENTER Telephone 14121 733·5200



## vee BUSINESS CONFIDENTIAL: Not to be released outside 

## vec without the written consent of Dr. R. L. Baron, Manager of Toxicology, Agricultural Products Company. Project Report 48-136 30 Pages December II, 1985

Aldicarb Solution (in DCM) Le50 Aerosol Acute Inhalation Toxicity Test Sponsor: Union Carbide Agrucultural Products Company



## * * * 

## * *
ABSTRACT**F~ve** 

### groups of five male and five female Sprague-Dawley albino rats were expcsed once for four hours to an aerosol of aldicarb solution in**di~hloromethane** 

### (DeM). The concentration of the individual components of the' test material (aldicarb, dichloromethane and methyl isocyanate) in the chamber atmosphere were determined by chromatographic methods. The mean aldicarb concentrations for the five exposures were 0.0463, 0.0087, 0.0060, 0.0020, and 0.00082 mg/L. The mean d1chloromethane (DCM) concentrations for the same exposures were 0.166, 0.025, 0.183, 0.094 and 0.043 mg/L. No methyl isocyante was detected in the chamber atmosphere. The mass median aerodynamic diameter for the aerosol particle size distributions ranged from 2.0 to 3.8 micrometers and the geometric standard ,deviations ranged from 2.5 to 5.0. Mortalities were seen in all but the 0.00082 mg/L male exposure group, and the 0.0020 and 0.00082 mg/L female exposure groups. Ataxia and tremors were seen 11 concentration groups during exposure. Addit ona c n cal signs observed in S'Ome groups on the day of exposure or during the postexposure period included periocular, perinasa1, and perioral wetness and encrustation, urogenital wetness, lacrimation, exophthalmos,' discolored and unkempt fur, an increased or decreased respiration rate, abdominal breathing, decreased motor activity, and a slow righting reflex. No clinical signs were observed in ,survivors during the second week of the postexposure period. Aloss of body weight or depressed body weight gains were observed in some animals on ppstexposure day 7. However, body weight gains were observed for all survivors on postexposure day 14. A discoloration of the lungs, perinasal encrustation anc staining, and eye abnormalities were observed at necropsy in some animals which died. The LC50 (with 95% confidence limits) for the combined sexes was 0.0039 (0.0028 to 0.0055) mg/L aldicarb.

BustiyRun**~esea,ch,Center** "" ' ' AJoint MeUon Instltute-Union Carbide Corporatio,n Op,eration,



### - ...'.'
. ',:'.','.:'.,.'"**'.~_.:.-:':**

### _ ,.::.."

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...:.::>,:':;",:,,i,'.::\**',:~i:~;~l;:'" '.::'~;:":':** **.;::;~,:'-:** ::"

### Report 48-136 Page 2 OBJECTIVE This study was designed to determine the acute inhalation Le50 to rats resulting from a four-hour exposure to aldicarb solution (in DCM)
MATERIALS AND METHODS 

### This study followed the specific protocol (BRRC Project 85-03-40150) and standard amendment prepared by the Bushy Run Research Center. Test Article Two, one-quart bottles of aldicarb solution (in DCM) (CAS Registry Number 116-06-03; Reference Number HTS 5819AA) were received on May 23, 1985, from Union Carbide Agricultural Products Company, Research Triangle Park, NC, assigned BRRC Sample Number 48-121 A 

### &B, and stored in Room 137. No chemical analyses were performed on the purity or composition of the material. Information received from the Sponsor stated the test material to be a formulation containing 37% aldicarb, 59% dich1oromethane, and a maximum of 5000 ppm methyl isocyanate (MIC). The Sponsor indicated the test material to be very stable at 20°C.
Animal Species, Source and Husbandry Male and female ZM/Sprague-Daw1ey albino rats (Zivic-Mi11er Laboratories, Inc., Allison Park, PA) were used. The age of the male and female rats on the day of exposure is listed in the following table:
Alicarb Expo·sure Date Age at Concentration (mg/L) Sex Received Exposure (days) 0.0463 M July 2, 1985 52 0.0463 F 

### July 2, 1985 56 

### 0.0087 M July 23, 1985 41 0.0087 F 

### July 16, 1985 54 0.0060 M July 23, 1985 47 0.0060 F 

### July 16, 1985 60 0.0020 M July 23, 1985 46 0.0020 F 

### July 16, 1985 59 0.00082 M July 16, 1985 56 0.00082 F 

### July 16, 1985 58

### Report 48-136 Page 3
The rats were assigned unique identification numbers by toe-clipping and were housed two or three per cage in 23.5 x 20 x 18 em high stainless steel wire mesh cages on carriers in Rooms 109 and l64E for the quarantine and postexposure periods. respectively. The rats were kept on a l2-hour photoperiod throughout the entire study period. A layer of Deotized Animal Cage Board- (Shepherd Specialty Papers, Inc., Kalamazoo. MI) was placed under each row.of cages. Pelletal feed (Certified Rodent Chow 5002. Ralston Purina Co•• St. Louis, MO) and tap water**(Mun~cipal** 

### Authority of Westmoreland County, Greensburg. PA) were available ad libitum except during exposure. Inhalation Chamber Design and Operational Characteristics The rats were individually housed in 21 x 12.5 x 18 cm wire mesh cages and exposed in a l330-1iter (approximate volume) rectangular with pyramidal top and bottom stainless steel and glass chamber in Room 137. The inhalation chamber design and operation are summarized in Table 1. Exposure Regimen Five groups of five male and five female rats were exposed once for four hours to an aerosol atmosphere of aldicarb solution (in DCM). No control exposures were performed.
Generation of the Test Material The aldicarb solution (in DCH) aerosol was generated using a Spraying Systems No. lA syphon spray set-up, (No. 1650 liquid nozzle and No. 64 air nozzle). The atomizer was inserted into the top of the inhalation chamber turret where the liquid aerosol was diluted to the desired concentration and dispersed throughout the chamber by filtered air. A laboratory pump was used to deliver the test solution to the atomizer. The test material used in the 0.0463 and 0.0087 mg/L exposures was undiluted. The extremely low flowrates of test material reqUired for the lowest three concentrations of aldicarb necessitated that the test material be diluted ten-fold (w/w) with DeM. To prevent clogging of the atomizer, an air piston (Bimba Model 091-NR) controlled by an electric valve (Skinner Model Bi4DKl075) and interfaced with a digital valve sequence programmer (Model DVSP-3) was used to actuate the clean-out needle of the atomizer at 10 second intervals. FiguLe 1 depicts a schematic diagram of the generation and exposure system. The operating conditions and the resulting chamber air flowrates are presented in the following table:

### Report 48-136 Page 4

Total Aldicarb Atomizer Chamber Exposure Air Air Concentration Atomizer Pressure Flowrate Pump* (mg/L) Type (psig) (L!min) Type and Setting 0.0463 Spraying Systems 15 300 FMI RP-G-6; 

### 1/8" piston; set at 200 0.0087 Spraying Systems 7 560 Sage pump with 30 ml syringe; set at 

### 1/100 and 5.5 0.0060 Spraying Systems 7 560 Sage pump with 30 ml syringe; set at 

### 1/100 and at 15.0 or 16.8 0.0020 Spraying Systems 7 560 Sage pump with 30 ml syringe; set at 

### 1/100 and 8.4 0.00082 Spraying Systems 7 

### 560 Sage pump with 30 ml syringe; set at 

### 1/100 and 4.2
*FMI Lab Pump (FMI Fluid Metering) Inc., Oyster Bay, NY)

Sage Model 355 Syringe Pump (Sage Instruments, Division of Orion Research, Cambridge MA) 

## I \ Target Concentration The target concentration for the first exposure was 0.2 mglL of aldicarb solution (in DCM) equivalent to approximately 0.05 mglL aldicarb. This concentration was selected based on the results of theD.O.T. aerosol test performed at BRRC on July 10, 1985 

### (BRRe Report 48-134). The target concentration for subsequent groups was based on the observed mortality of previous groups. Chamber Concentration Analysis Since the DCM concentrations posed an 1nsignficant acute toxicity hazard in comparison to aldicarb, the concentrations presented for this report and the LCSO calculations were based on the aldicarb concentrations, and not the a1dicarb solution (in DCM) concentrations. The concentration of aldicarb, dichloromethane t 

### and methyl isocyanate in the exposure chamber was determined during the five exposures by , . 

### chromatographic methods. The total test materIal concEmtrat1on**of~ldicarb** 

### solution (in DCM) in the exposure chamber atmosphere was calculated by adding the individual component ccincentrations.A.complete desCrip.t1"Ilof theeL'" 

### analytical methods used is given .inthe**Analyt~alCliemisdyReport,** 

### (Appendix 1):
.. ;' '; c'. -,' -.; 

## :3:'<Report 

### 48-136 Page 5
The nominal concentration of the aldicarb solution (in DCM) was determined by dividing the total weight of the solution generated by the volume of air which passed through the chamber during the exposure period. The value for total weight was estimated by multiplying the number of milliliters used by the specific gravity (1.23 g/ml) of the test material. Particle Size Distribution The particle size distribution of the aldicarb solution (in DCM) aerosol was not obtained for the 

### 0.0463 mg/L exposure due to the short exposure time. Only one particle size sample was obtained for all other groups, generally due to the long sample collection times. A Sierra 8-stage cascade impactor (Series 

### 210, Sierra**IU6tru~ents,** Inc., Carmel Valley, CA) was usee to obtain the chamber samples of the aerosol. Sierra C-220 WH filters were used to collect the aerosol on the stages and the filters were assayed gravimetrically. The mass**m~dian** aerodynamic diameter and geometric standard deviation were determined from log probability plots of the cumulative percent mass collected on each impactor 9tage. Temperature and Relative Humidity The temperature and relative humidity in the animal housing rooillwere recorded continuously with a seven-day recording hygrothermograph (Cole-Parmer Instruments, Chicago, IL). During the exposure the temperature was monitored with a minimum-maximum thermometer (Abbeon Cal. Inc., Santa Barbara, CA) and the relative humidity was monitored with an'Airguide Humidity Indicator (Airguide Instrument Company, Chicago, IL). Animal Observation All animals were observed on the day of exposure, and for 

### 14 days following exposure for signs of toxic effects. Body Weights The animals were weighed prior to exposure and on post-exposure days seven and fourteen. The change in body weight was calculated by subtracting the pre-exposure values from each successive weight. Necropsy

. The animals were sacrificed following methoxyflurane anesthesia.**Th~.** rats were exsanguinated by severing tile brachial blood vessels and a complefe""
. , necropsy was performed. The lungs, liver, kidneys, nasal passages, and any**a~normal** tissues were placed in a fixative and saved for,p-0Sl.sible,fuf:urec histologic examination. The following table relates the exposure and terminal**~acrlf1ce** dates:**:.~**Repcrt 

### 48-136 Page 6
Date of Ter'llinal Sacrifice 

# • * August 

### 14, 1985 August 

### 13, 1985 August 12, 1985 Exposure Date july 

### 12, 1985" July 25, 1985 July 31, 1985 July 

### 30, 1985 July 29, 1985**~~dicarb** Exposure Concentration (maIL)

*No terminal**sacrific~-al1** animals died during exposure or 14-day postexposure

observation period. Statistical Procedures The mean and standard deviations of the body weights, body weight changes, and exposure concentrations were calculated. No statistical comparisons were made. The 

### LC50 was determined by a modified**met~od** of Finney's probit analysis (1952) • Storage of Records The final report, tissue specimens, and all raw data are retained in the Archives of the Bushy Run Research Center. RESULTS Chamber Concentration Analytical results are presented in the Analytical Chemistry Report (Appendix 1). The mean aldicarb concentration for the five exposures were 

### 0.0463, 0.0087, 0.0060, 0.0020 and 

### 0.00082 mg/L. The mean dichloromethane (DCM) concentrations for the same exposures were 

### 0.166, 0.025, and 

### 0.183, 0.094 and 

### 0.043 mg/L. No methyl isocyanate was detected in the chamber atmosphere. The mean measured concentration of aldicarb solution (in DCM), calculated by addition of the individual components, and the nominal concentrations for the five exposures are presented in Table 2. Particle Size Distribution The resu1t·s of the impactor samples are presented in the follOWing table: Aldicarb Exposure Sample Sample Mass Median Concentration Flowrate Duration Aerodynamic (mg/l) (Llmin) (min) Diameter (mm) 

### 0.0463 --*-- 

## * * 0.0087 8.8 45 3.4 0.0060 8.8 

### 90 3.6 0.0020 8.8 180 3.8 0.00082 8.8 180 2.0 GeometriC Standard Deviation 

## * 4.1 2.5 3.9 

### 5.0 *The particle size distribution of the aldicarb solution (in 

### DeM) aerosol was

not determined for this exposure level. All animals died before a'.'sample of ':"i.;.:; the aerosol could be obtained. Figures'2 through 5 present a graphical presentation of these

### Report 48-136 Page 7 Humidity and Exposure Chamber Conditions The temperature and relative humidity in the animal housing room ranged from 6S to 72°F and 46 to 69%, respectively, throughout the study period. The temperature and humidity data for the exposure chamber are presented in Table 1. Animal Observations Animal observations during the exposure and postexposure periods are summarized in Tables 3 (male rats) and 4 (female rats). Clinical signs of toxicity were observed in all exposure groups. Clinical signs observed during exposure for the 0.0463 and 0.0087 mg/L exposure groups included ataxia, tremors. hypoactivity. an increased respiration rate, lacrimation, exophthalmos, and the presence of a red periocular encrustation. The only clinical signs of toxicity observed during exposure for the 0.0060, 0.0020. and 0.00082 mg/L exposure groups were ataxia and tremors. Addition signs observed following exposure or during the postexposure period for the three lower exposure concentration groups included periocular. perinasal. and perioral wetness, discolored and unkempt fur, decreased motor activity, a slow mid-air righting reflex, abdominal breathing. and a decreased respiration rate. No clinical signs of toxicity were observed in survivors during the second week of the postexposure period. Mortality The folloWing table presents a summary of the mortality observed during the study:
Aldicarb Exposure Time of Death Concentration During Total (mg/L) Sex Exposure Postexposure Day 1 

### Incidence 0.0463 M 

### 5 0 

### 515 0.0463 F 

### 5 0 5/5 0.0087 M 5 

### 0 

### 515 0.0087 F 5 

### 0 

### 515 0.0060 M 0 

### 2 

### 2/5 0.0060 F 1 2 

### 3/5 0.0020 M 

### 0 I 

### lIS 0.0020 F 

### 0 0 0/5 0.00082 M 

### 0 0 O/S 0.00082 F 

### 0 0 0/5
The**LC~~** 

### (with 95% confidence limits) for males was determined to be
. 0.0038 (0.0021 to 0.0071) mg/L; for females 0.0044 {O.0029toO.0069)mg/Li and for the combined sexes it was 0.0039 (0.002atoO.00S5) mg/L.'Xhe slopes for the dose-mortality curves for male rats, female rats,and thecombiried
. sexes were .2.22, 3.86 and 3.04, .,respectively.
. , 'iReport 48-136 ·Page 8 Body Weights The individual body weights and body weight changes are summarized in Tables 3 (male rats) and 4 (female rats). A loss of body weight was observed in some rats on postexposure day 7 in the 0.002 mg/L male, and 0.006 and 0.002 mg/L female exposure groups. DeprEssed body weight gains were observed for two female rats from the 0.00082 mg/L exposure group on postexposure day 7. Body weight gains were observed for all survivors on postexposure day 14. Necropsy Necropsy findings are summarized in Tables 5 and 6. Individual animal findings are provided in the pathology sheets in Appendix 2, The principal gross lesions observed in animals vhich died included a disc.,loration of the lungs, perinasal encrustation and staining, and eye abnormalities (periocular encrustation. periocular staining, lenticular opacity and corneal opacity). No significant dose-related macroscopic lesions vere observed in rats sacrified at the end of the l4-day observation period. The enlarged cervical lymph nodes observed in some of the survivors and mortalities are a frequent finding in this strain and are not considered to be exposure related.
DISCUSSION The aldicarb chamber concentrations for the five aerosol exposures were 0.0463. 0.0087. 0.0060, 0.0020, and 0.00082 mg/L. The dichloromethane C0ncentrations for the same exposures were 0.166, 0.025, 0.183, 0.094 and 0.043 mg/L. No methyl isocyanate was detected in chamber atmospheres. Complete mortality**~as** observed in the 0.0463 and 0.0087 mg/L aldicarb exposure groups. The only groups with no mortalities were the 0.0020 mg/L males, and the 0.00082 mg/L exposure group (both sexes). Ataxia and tremors vere seen in all groups during exposure. In addition, clinical signs of ocular and nasal irritation, perioral wetness and encrustation. respiratory difficulties, and**~oordination** loss vere observed in some exposure groups on che day of exposure or during the first week of the postexposure period. Hovever, no clinical signs vere observed in survivors during the second week of the postexposure period. Although Bome animals were observed to have a loss of body weight or depressed body weight gains on postexposure day 7, body weight gains vere observed for all survivors on postexposure day 14. A discoloration of the lungs, perinasal encrustation and staining, and eye abnormalities were observed in some animals which died. The LC50 for aldicarb (vith 95% confidence limits) for male rats was 0.0038 (0.0021 to 0.0071) mg/L; for females 0.0044 (0.0029 to 0.0069) mg/L; and for the combined sexes it**wa~** 0.0039 (0.0028 to 0.0055) mg/t. Project Tearn 

### D. 

### J. Nachreiner, 

### B.S. D. W. Fait, B.S. M. L. Steel, AALAS, Cert. I D. 

### R. Klonne, Ph.D. 

### M. 

### J. Cardella. , AALAS Cert. P. A. Baker, RN II Study Director Master Technologist Senior**Technolog1s~ ProjectMana~er;**  . Animal' Care.Supervisor:.

.,....'. Necropsy SuperVisor. .": ,.'.,

### Reviewed and Approved by: Report 48-136 Page 9 

# LCl\t<.i)"? 

# J /-,

# J

..**'1.L<.~** / I .'

.. 

### Donald 

### J. Nachreiner. 

### B.S. Date Study Director 

# ?
....,......,**~i~'** !:.·(N t".



### Dennis 

### R. Klonne. Ph.D. Project Manager 

### ---::::'

# ~/'7<£=-.~

Fred R. Frank. Ph.D. Director 

### REFERENCES 1/"/'·
...:-:; Date 

# ;zft</ Date



### 1. Finney. 

### D. J. (1952). Probit Analysis, 2nd 

### Ed., Cambridge University Press, London, England. WPC/rkk/0909B-4 11-11-85

### Report 48-136 Page 10 Table 1 

### Inhalation Chamber Design and Operation Aldicarb Solution (in DCM) LeSe Aerosol Acute Inhalation Toxicity Test CHAMBER Location: Room 137 Construction: The chamber was made of stainless steel and glass. Shape: Rectangular with pyramidal top and bottom Dimensions: Height

- 1.32 meter Length

- 0.91 meter Width

- 0.85 meter Total Volume

- Approximately 1330 liters Airflow Measurement: 

### An orifice plate was positioned in the exhaust duct of the chamber and was connected to a Dwyer Magnehelice 

### pressure gauge (chamber airflow). A critical orifice. calibrated with a Singere 

### dry gas test meter. was used for the impactor samples. Test Article Generation: A Spraying Systems Atomizer was used to generate**aerosol~** 

### of aldicarb solution (in 

### DCM). Undiluted test material was used for the 0.0463 and 0.0087 mg!L exposures; for all other exposures, the test material was diluted ten-fold (w!w) with DCM.

Chamber

Number Chamber Atmosphere Conditions: Aldicarb Exposure Concentration (mg!L) Mean ± 

### SD Temperature* (OF) 

### Mean ± 

### SD Relative Humidity** 

### (%) 37-1 37-1 37-1 37-1 37-1 0.0463 0.0087 '0.0060 0.0020 0.00082 75 ± 0 72 ± 1 71 ± 0 

### 70 ± 

### 0 72 ± 1 

### 33 ± 

### 1 34 ± 

### 1 35 ± 0 

### 35 ± 

### 0 36 ± 

### 1

II: 

### Determined using an Abbeon minimum-maximum thermometer. ** Determined using an Airguide Certified Hygrometer. WPC/rkk/0909B-2 10-03-85

### Report 48-136 Page 11

Table 2 Nominal Exposure Concentrations Aldicarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test



### == Aldicarb Sol-* Total Aldicarb 501-111: Aldicarb ution (in DCM) Weight Chamber ution (in DCM) Exposure Exposure Exposure of Test Air Nominal Concentration Concentration Duration Material Flowrate Concentration (mg/L) (mg/L) (min) Used (grams) (L!min) (mg/L) 0.0463 0.2123 60 9.90 300 0.5500 0.0087 0.0337 240 9.84 560 0.0732 0.0060 0.1890 240 22.14 560 0.1647 0.0020 0.0960 240 15.87 560 0.1181 0.00082 0.0431 240 5.23 560 0.0389



## * The aldicarb solution (in DCM) concentration in the exposure chamber

atmosphere was calculated by adding the individual component concentrations of aldicarb and DCM. No methyl isocyanate was detected in the chamber atmosphere.


## ** The a1dicarb solution (in DCM) used for the 0.0463 and 0.0087 mg/L exposures was undiluted; for the remaining 3 concentrations the test material was diluted ten-fold (w/w) with DeM. WPC/rkk/0909B-3 11-11-85

### Individual Animal Aldicarb Solution Table 3 

### Report 48-136 Page 12



## IL Aldicarb Male Rats*) Inhalation Toxicity Test

Animal Number Weights (g) 

### at Day 0 85-16768 283 85-16765 290 85-16764 280 85-,16767 272 85-16766 260
Mean: 277 

### Standard Deviation: 11 

### Days to Death During exposure During exposure During exposure During exposure During exposure Mortality Ratio: 5/5 Group Observations: Clinical signs observed during the exposure were ataxia, tremors, an increased respiration rate, hypoactivity, lacrimation, and a red periocular discoloration. Mortalities occurred within 60 minutes of exposure. Exposure Date: July 12, 1985 

## * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison Park, PA, were used. (Continued)

### Report 48-136 Page 13

Table 3 (Continued) Individual Animal Aldicarb Solution /L Aldicarb Male Rats*) Inhalation Toxicity Test Animal Number Weights (8) 

### at Day 0 Days to Death 85-17038 256 During exposure 85-17040 267 During exposure 85-17036 284 'During exposure 85-17039 274 During exposure 85-17037 294 During exposure Mean: 275 Mortality Standard Deviation: 

### 15 Ratio: 5/5 

### Group Observations: Clinical signs observed during the exposure were ataxia, tremors, an increased respiration rate, hypoactivity, lacrimation, exophthalmos, and a red periocular discoloration. Mortalities occurred between 25 and 240 minutes of exposure. Exposure Date: July 25, 1985 * Albino rats (ZM/Sprague-Daw1ey), Zivic Miller Laboratories Inc., Allison Park, FA, were used. (Continued)

### Report 48-136 Pag@ 14

Table 3 (Continued) Individual Animal Body Weight Data (0.0060 mg/L A1dicarb Male Rats*) Aldicarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test Animal Weights (g) at Day Body Wt. Change Number 0 7 14 7 14 Days to Death 85-17442 248 280 362 32 114 14 (S) 85-17448 260 276 353 16 93 14 (S) 85-17447 254 1 85-17449 255 283 357 28 102 14 (S) 85-17451 272 1
Mean: 258 280 357 25 103 Mortality Std. Dev. : 9 4 5 8 11 

### Ratio: 2/5 Group Observations: Clinical signs observed**d~ring** 

### exposure were ataxia and tremors. Additional signs of toxicity observed**fo11o~ing** 

### exposure were periocular, perinasa1. perioral, and urogenital wetness, and decreased motor activity. During the postexposure period. signs observed were periocular, perinasa1, and perioral encrustation, discolored and unkempt fur, decreased motor activity, a slow mid-air righting reflex, abdominal breathing. and a decreased respiration rate. No clinical signs Gf toxicity were observed on postexposure days 5 through 14. Exposure Date: July 31, 1985 (S) • Sacrificed on August 14, 1985 

## * Albino rats (ZM/Sprague-Daw1ey), Zivie Miller Laboratories Inc., Allison Park, PA, were used. (Continued)

### Report 48-136 Page 15

Table 3 (Continued) Individual Animal Body Weight Data (0.0020 mg/L Aldicarb Male Rats*) Aldicarb Solution (in DCM) LCSO Aerosol Acute Inhalation Toxicity Test
AnImal**!~ights** 

### (g) at Day Body Wt. Change Number 0 

### 7 14 7 14 Daxs to Death 85-17408 250 246 333 -4 83 14 (S) 85-17407 242 1 85-17406 254 285 37? 31 118 14 (S) 85-17410 247 273 357 26 110 

### 14 (S) 85-17409 254 271 356 17 102 14 (5)


### Mean: 249 269 

### 355 18 103 Mortality Std. Dev. : S 16 16 15 15 Ratio: 1/5 Group Observations: Clinical signs observed during exposure were ataxia and tremors. Additional signs of toxicity observed following exposure were periocular, perinasal, peroral, and urogenital wetness, decreased motor activity, abdominal respiration, and a decreased respiration rate. During the postexposure period, signs observed included periocular, perinasal, and perioral encrustation, discolored and unkempt fur, ataxia, decreased motor activity, a slow mid-air righting reflex, abdominal breathing, and a decreased respiration rate. No clinical signs of toxicity were observed in one animal by postexposure day 1, in 3 animals by postexposure day 2, and in all survivors on postexposure days 6 through 14. Exposure Date: July 30, 1985 (5)

- Sacrificed on August 13, 1985 * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison Park, PA, were used. (Continued)

### Report 48-136 Page If)

Table 3 (Continued) Individual Animal Body Weight Data (0.00082 mg/L Aldicarb Male Rats*) Aldlcarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test Animal Weights (g) at Day 

### Body Wt. Change Number 

### 7 14 

### 7 14 Days to Death 85-17249 290 307 386 17 

### 96 14 (S) 85-17250 300 337 396 37 96 14 (5) 

### 85-17251 294 334 399 40 105 14 (5) 85-17242 300 338 407 38 107 14 (5) 

### 85-17240 300 329 404 29 104 14 (5)
Mean: 297 329 398 32 102 Mortality Std. Dev. : 5 13 

### 8 9 

### 5 Ratio: 0/5 Group Observations: Clinical signs observed during exposure were ataxia and tremors. No clinical signs of toxicity were observed following exposure. During the postexposure period, clinical signs were observed only on postexposure day I for 3 rats. The clinical signs observed were periocular, perinasal, and perioral encrustation, discolored and unkempt fur, decreased motor activity, abdominal breathing, and a decreased respiration rate. Exposure Date: July 29, 1985 (5) = 

### Sacrificed on August 12, 1985 

## * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison Park, PA, were used.

WPC/rkk/0909B-2 10-03-85

### Report 48-136 Page 17

table 4 IndiYidual Animal Aldicarb SolutIon ht Data (0.0463 m/L Aldicarb Female Rats*) LC50 Aerosol Acute Inhalation Toxicity Test

Animal Number Weights (g) 

### at Day 0 Days to Death 85-16782 237 During exposure 85-16785 261 During exposure 85-16784 261 During exposure 85-16786 288 During exposure 85-16783 252 During exposure Mean: 260 Mortality Standard Deviation: 19 Ratio: 5/5 Group Observations: Clinical signs observed during the exposure were ataxia, tremors, an increased respiration rate, hypoact1vity, lacrimation, and a red periocular discoloration. Mortalities occurred within 60 minutes of exposure. Exposure Date: July 12, 1985 

## * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison Park, PA, were used. <Continued)

### Report 48-136 Page 18

Table 4 (Continued) Individual Animal Body Weight Data (0.0087 mg/L Aldicarb Female**Rats~)** 

### Aldicarb Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test Animal Number Weights (g) 

### at Day 0 Days to Death 85-17083 210 During exposure 85-17084 218 During exposure 85-17085 207 During exposure 85-17086 228 During exposure 85-17087 203 During exposure Mean: 213 Mortality Standard Deviation: 10 Ratio: 515 

### Group Observations: Clinical signs observed during the exposure were ataxia, tremors, an increased respiration rate, hypoactivity, lacrimation, exophthalmos, and a red periocular discoloration. Mortalities occurred between 25 and 240 minutes of exposure.**Exposu~~** 

### Date: July 25, 1985 Albino rats (ZM/Sprague-Daw1ey), Zivic Miller Laboratories Inc., Allison Park, PA, were used. 1, ) 

### (Continued)Report 48-136 Page 19 Table 4 (Continued) Individual Animal A1dicarb Solution ht Data (0.0060 m

### IL Aldicarb Female Rats*) LC50 Aerosol Acute Inhalation Toxicjty Test Body Wt. Change

7 14 Days to Death During exposure

1 -8 

### -11

-10

2 14 11



### 12



### 2 14 (S) 14 (5)

1
.' Mortal!ty

Ratio: 3/5 Group Observations: Clinical signs observed during exposure were ataxia and tremors. Following exposure, ataxia and tremors were not observed; however, additional signs observed following exposure were periocular, perinasa1, perioral, and urogenital wetness, "decreased motor activity, and a decreased respiration rate. During the postexposure period,signs observed were periocular, perinasa1, and perioral encrustation, discolored and unkempt fur, decreased motor activity, a slow mid-air righting reflex, abdominal**breathi~g,** and a decreased respiration rate. No clinical signs of toxicity were observed on postexposure days 5 through 14. Exposure Date: July 

### 31, 1985 (S) = Sacrificed on August 14, 1985 

## * Albino rats (ZM!Sprague-Dawley), Zivic Park, PA, were used. Miller Laboratories Inc., Allison; ,'J , ",;**-:...~.** (Continued)

### Report 48-136 Page 20

Table 4 (Continued) Individual Animal Bod Wei ht Data (0.0020 m /L Aldicarb Female Rats*) Aldicarb Solution (in DCM LC50 Aerosol Acute Inhalation Toxicity·'TeS't
Animal Weights (g) at Day Body Wt. Change Numbel: 0 

### 7 14 7 14 Days to Death 85-17104 234 244 280 10 46 

### 14 (5) 

### 85-17103 226 213 259 -13 33 14 (S) 

### 85-17105 229 240 247 11 

### 18 14 (5) 

### 85-17106 239 226 271 -13 32 14 (5) 

### 85-17107 236 245 283 9 47 14 (S)
Mean: 233 234 268 0.8 35 Mortality Std. Dev. : 5 14 15 13 

### 12 Ratio: 0/5 Group Observations: Clinical signs observed during exposure were ataxia and tremors. Additional sign& of toxicity observed following**expoe~re** 

### were periocular, perinasal, peroral, and urogenital wetness, decreased motor activity, abdominal respiration, and a decreased respiration rate. During the postexposure period, signs observed included periocular, perinasa1, and perioral encrustation, decreased motor activity, abdominal breathing, and a decreased respiration rate. No clinical signs of toxicity were observed in two animals by postexposure day 1, in 4 animals by postexposure day 2, and in all survivors on postexposure days 6 through 14. Exposure Date: July 30, 1985 (5) C 

### Sacrificed on August 13, 1985 

## * Albino rats (ZM/Sprague-Dawley), Zivic Miller Laboratories Inc., Allison Park, PA, were used. (Continued)

# "
,

### R:~,p'oit 48-136 ." Page 21

"
Table 4 (Continued) Individual Animal Body Weight Data (0.00082 moIL**fi~U~Ca[O** Female Rats*) Aldicarb Solution (in DeM) Le50 Aerosol Acute Inhalation Toxicity Test
Animal Number Weights (g) at Day 

## o 7 14 Body Wt. Change 7 14 Days to Death 85-17099 85-17098 85-17100 85-17101 85-17102
Mean: Std. Dev.: 217 224 

### 221 

### 244 211
223

12 217 232 231 255 212
229

17 261 271 265 290 244
266

17 

## o 8 10 11

1
6 5 44 

### 47 44 46 33
43

6 14 (5) 14 (5) 14 (5) 14 (5) 14 (S) Mortality Ratio: 0/5 Group Observations: Clinical signs observed during exposure were ataxia and tremors. No clinical signs of toxicity were observed following exposure. During the postexposure period, clinical signs were observed only on postexposure day 1. The clinical signs observed were periocular, perinasal, and perioral encrustation, urogenital wetness, discolored and unkempt fur, and decreased motor activity. Exposure Date: July 29. 1985 (S) 

## = Sacrificed on August 

### 12, 1985 

### * Albino rats (2X/Sprague-Dawley), Zivic**Mill~r** Laboratories Inc., Allison Park, PA, were used.

WPC/rkk/0909B-3 11-11-85...**()R.r~~NSI** F).n.d.l.ngs

_ NASAL CAVITY. Nl;L

IPerill<l!;;! I elll:ruslation /Perinasal staining TRACIII-:A, NGL

/Mucous f HIed LUNGS. NGL

/Colllr change BRONCHI, NGL I.J VEl{. NGL

IC(,lur**chiln~e** KIDNI-;YS, NGL**IlIyd~lll\eph~o~is**

/Cyst, cortical**su~face C~~RV**  ICAL I.YMI'H**NOlll~,** Nl;L ISize change 

### /eo I or**ch;ll\~e** EYES. N(;L

IC"rl1('ill ('pi!e I ty**·/J.entlculil~** upncity

.**/l'e~inclllilr** cncrllstntion II'eriocu1.1r stilll1ing Nt:!. = No gross lesions. Table 5**Aldicn~b** Solution (in DCM) LC50 Aerosol Acute Inhalation Toxicity Test**F~equency** of Gross**Findin~** for RatH that Died
Mnles

-------**~gj.llle~~_I.~I}_~·.;I_rj?** O.04h3 0.OO!l7 0.0060 0.0020

5/';* 

### 20 1/2 0/1 0/5 1/5 1/2 0/1 0/5 2/5 0/2 1/1 5/5 5/5 2/2 1/1 0/5 0/5 0/2 0/1 2/5 0/5 0/2 1/1 3/5 5/5 2/2 0/1 5/5 S/5 2/2 1/1 5/5 5/5 0/2 1/1 0/5 0/5 2/2 0/1 4/5 3/5 2/2 0/1 1/5 2/5 0/2 1/1 0/5 0/5 0/2 0/1 S/S 4/5 2/2 1/1 DiS 1/5 0/2 0/1 0/5 0/5 0/'2. 0/1 0/5 0/5 0/2 0/1 2/5'; r./5 O/Z 0/1 4/5 5/5 1/'2. 0/1 2/5 1/5 2/Z 1/1 1/5 4/5 0/2 

### OJ] Females**~/liter** of Aldicarb 0.0463 0.0087 0.0060

5/5 4/5 3/3 0/5 1/5 0/3 0/5 0/5 0/3 5/5 5/5 2/3 O/S 0/5 1/3 2/5 0/5 1/3 3/5 5/5 2/3 5/5 5/5 3/3 5/5 5/5 3/3 0/5 0/5 0/3 4/5 4/5 3!J 1/5 1/5 0/3 1/5 0/5 0/3 4/5 5/5 1/3 1/5 0/5 1/3 0/5 0/5 1/3 0/5 0/5 0/3 2/5 0/5 O/) 3/5 5/5 2/3 0/5 115 2/3 4/5 3/5 0/3

*Numeracor equals number of rats with specified finding.

1ll!llllminntor CI!U11lS numher of r:lts for which specifi(',t orgnn was rxnmined. 0-;:, ;u • :J r:- 'r.'t:: ": .:



### .., r..; r· 1_ L'

0:: I**.~.** :JLlh I

.. () Aldicarb Solution (in DCM) LC,50 Aerosol Acute Inhalation Toxicity Test**.r:..r~(~~l!.I1:~y_.!~r_**Cross**fl~~IJ!~;_~\.:l** ts**~~** r1 f iced at 14 [)ays Mill!!5 Females mg/liter of Alclicorh mg/llter of Aldicarb**ORGA~S/Flndings** 

### O.OOhO O.OO:.!O O.UOU82 

### O.OOhO 0.0020 0.00082

------ NASAL CAVlTY, NGL J/3* 4/4 5/5 2/2 SIS 5/5 TRACHEA, NCL 3/3 4/4 5/') 2/2 5/5 S/5 LUNGS, NGL 3/3 3/4 5/5 2/2 5/5 5/5

/Color change 0/3 1/4 0/5 0/2 0/5 0/5 BRONCHI, NGL 3/3 4/4 5/5 2/2 5/5 5/5

_ LIVER, NGL 31J 4/4 5/5 2/2 5/5 5/5**KlDN~:YS,** NGL 3/3 0/4 3/5 2/2 5/5 3/5

/Hydronephrosis 0/3 4/4 2/5 0/2 0/5 2/5 CERVICAL LYMPH NODES, NGL 3/3 J/4 4/5 2/2 4/5

- 5/5

/S i?e**Cll<ln~e** 0/3 1/4 1/5 0/2 1/5 0/5 EYE, N(;L J/3 4/lj 4/5 2/2 5/5 5/5

/}.cnt**il:~~)a** f{-'pae i ty 0/3 0/4 1/5 0/2 0/5 0/5

-- - Nl;!, ,=. No RrosH lesions. *NlImerator equals 1IIIIIIher of rllts with !Jpecified finding.

lll!llu1l1inatur f.lqu.l!s l1u1I11>l'f of rats fur which specified org,ln was examined.

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# ,<0«'.**:V~i:1~ ~~** 

# ..**~.-.**R. O. 4, Mellon Road, Export, Pennsylvania 15632 

# • • . : I .,. 

## BUSHY RUN 

# RESEARCH Report 48-136**Pilg~'** .!':! 

# CENTER Telephone 14121 733·5200

Compliance with FIFRA Good Laboratory Practices
This study was conducted in accordance with current Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) Good Laboratory Practices (GLP).

\'"PC/rkk/0909B-2 

### 10-03-85 D. J. Study I,i" ,. . '
.. .r..,,.........**.:.~ 1~**  0

__ Nachreiner, B.S. Director f i: DateReport 40-136 •. Page 3u

R. D. 4, Mellon Road. Export, Pennsylvania 15632 

# • ,,"

. : 

# ..**~.,** 

# BUSHY RUN RESEARCH CENTER Telephone 14121 7:13-5200 Qualitv Assurance Unit Studv Inspection Sumroarv
rest Substance: Aldicarb in**DC~** Study: LC 50 Acute Aerosol Study Director: D. J. Nachreiner, B.S. The Quali:y Assurance**~nit** of BRRC conducted the inspections liste=**c~2~~** and rep=rted the**res~lts** to the study director and to management on the**~a:fE** incicated. It is the practice of this Quality Assurance Unit to report**t~~ re5~lts**  of 'each inspection to both the study director and management. 1:15:-<.'::::ic;'1 Da:e Date 

### QAL**Re~~rt** Issue: To**St~~\ Direct~~** **2~ X~~az~~=~:**  12-20-83 Standard Protocol and Amendment 12-20-83 1-26-84
7-2-85 Standard Protocol Amendment 7-2-85 7-8-85 10-9 to 1Q-U-85 Final 

### Raw Data and Final Report 10-11-85 12-9-85

. ' ..' '. .' /). '.

. ',','**"':.~:'.~"** 

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...



### APPENDIX 1 Exposure Chamber Atmosphere Analyses for the Aldicarb Solution (in**DC~)** 

### Acute LC50 Aerosol Inhalation Study on Rats 

### (9 Pages)Rt>pClrt "R-136 '

APPENDIX 1 Exposure Chamber Atmosphere Analyses for the Aldicarb Solution (in**DC~)** Acute LC50 Aerosol Inhalation Study on Rats
SUMMARY The concentrations of aldicarb, dichloromethane 

### (DeM), and methyl isocyanate (MIG) in the exposure chamber atmosphere were determined during**t~e** five exposures by chromatographic methods. The aldicarb was scrubbed from**t~e** chamber atmosphere using impingers; the solutions were then analyzed by reve=se phase liquid chromatography. Dichloromethane and methyl isocyanate concentrations were determined by direct analysis ,of the chamber atmosphere usinJ gas chromatographs equipped with flame ionization detectors. A gas chromatograpt equipped with a nitrogen-phosphorus detector with sensitivity to detect ppb concentrations of methyl isocyanate was used for industrial hygieuc monitoring. The mean aldicarb concentration (± SD) for the five exposures were 0.82 (± 0.100), 

### 2.0 (± 0.15), 6.0 (± 0.61), 

### 8.7 (± 1.38), and 46.3 ug per liter. The**mea~** dichloromethane (DCM) concentrations (± 

### SD) for the same exposures were 12.7 

### (! 5.76), 27.7 (± 2.71), 54.0 (= 5.19), 7.4 (= 1.4B), and 49.1 (= 12.97) ppm. These dichloromethane concentrations are equivalent to 43, 94, 183, 25, and 166**~g** per liter.**~o** methyl isocyanate was detected in the chamber atmospheres. The detection li=it was 0.9 ppm. CHEMICALS Aldicarb in Dichloromethane (DCM) Test Material: BRRC Number 48-121 A and B,**HrS5819;_~.** Formulation containing 37% aldicarb, 59% dichloromethane,anc 5000**pp~ ~ahimum**  methYl. isocyanate (MIC) and provided by rnion Carbide· Agricultural Products Company. Aldicarb: 2-metnyl-2-(methylthio)-propionaldehyde o-(methy1 carbamyl ox:=c) Aldicarb Standard: BRRC Number 48-117 A and B, HAS3813AA. A 99.4', a1dicarb standard provided by Union Carbide Agricultural**Produ~ts Compa~y.**  Methyl Isoc;anate Standard: VICI Metronics (Santa Clara, california)**standa~:** rate**per~eation** tube. Part Number 110-150-7473. Di.ch1oromethane Standard: BRRC Number 46-283. reagent. AcetonitrUe :BRRC Number 48-..71, \o'ater: Fl1teredwater obtained .," :'th.';"'(lrt _":,-: ',' APilL':H! i :.: : -2-
INSTRUHENTATIOl': Aldicarb Analysis High pressure liquid chromatography was performed using a**Spe~tra-Phys1cs** SP BODO B liquid chromatograph equipped with an SP 8400 ultraviolet/visible detector and an SP 8000 Data System. Dichloromethane Analysis A Perkin-Elmer Sigma 2000 gas chromatograph 

### eGC) equipped with aflame**io~ization** detector was used to monitor the DCM vapor concentration in the chamber atmosphere. Peripheral equipment included a VICI Va1co Instruments automatic gas sampling system and a Spectra-Physics SP-4200 Computing Integrator•**~ethyl** Isocyanate Analysis A Perkin-Elmer 3920B gas chromatograph equippeJ**~ith** a flame ionization detector was used to monitor the 

### MIC concentration**i~** the chamber atmosphere. A Spectra-Physics series 4000 central processor, a data interface, and a PerkinElmer automatic gas sampling system were used for the analyses. A 

### VICI**~etronics** Model 450 Dynaca1ibrator was used to prepare MIC gas standards. The 

### MIC hygiene monitoring was performed using similar instrumentation to that used for the 

### MIC chamber atmosphere analyses except that a nitrogen/phosphorus GC detector was used instead of the flame ionization detector. ANALYSIS METHODS Aldicarb The reverse phase high pressure liquid chromatography method is summarized as follows: 1. Colultn:
2.**Mobil~** PhsBe: 3, Flow Rate: 

### 4. Injection Volume: 

### 5. Ultraviolet Detection: 6. Flo\-; :-lode: 7. Preco1umn Ct!'ard Cartridge:


### 8
.. Retention Time A1ltech Rosil**C~** BUReversecPhase (250 x 4.6 mm)"' 60%**Water/40~** Acetonitrile (v/v) 1.5 mL/minute 

### 10 ).lL 210 nm Constant Brownlee RP-18 SPHERI,..5 :-IF,LC'', .3 "C'!!LX 4,'6 'mm'**"~"** 5 minutesi-:l' ;", r: ;.,;.- I 3i) 1\1'1',-,11,: i ;.: I -3- Dich]oromethan€' The gas chromatographic method is summarized as follows: 1. Column:

2. Column Temperature: 3. Gas Sample Valve TeJllperature: 4. Flame Ionization Detector Temperature: 

### 5. Nitrogen Carrier Flow: 6. Gas Sample Loop: 

### 7. Retention Tim€': Methyl Isocyanate 10% SP2100 on 80/]00 mesh SupelcoportC (6 ft. x 1/8 in. stainless steel) 60·C 60·C

20 mLlminute 1 cc stainless steel 0.91 minute!':

The gas chromatogra?nic method is summarized as follows: 1. Colu!!;n: Chromosorh 101 

### (3 ft. x 1/4 in. stainIess steel) 

### 2. Colul::1 Temperature: 135·C 

### 3. Gas Sample Valve TetJperature: 150·C 

### 4. Flal:E Ionization Detector ::-e:::perature: 200·C 5. Nitrogen Carrier Flo\..': 20**m~/minute** 6. Gas Sample Loop: 1 cc stainless steEl 

### 7. Retention Time: 129 seconds The 

### MIe**~ygiene** monitoring gas chromatographic**~ystern** had similar**op€'r2::~~** parameters to those of the methyl isocyanate chamh€'r atmosphere**analy~is** parameters. The nitrogen/phosphorus detector was o..erated in the**nitro~er c~:~**  and the carrier flowrate was 40 mt/minute. A 4-foot Chrolllosnrh 10] column**~~~** used.K\·;1,,-... r t !.**~~-** /·.;JrlVfhJ i ;.: ! -4-**CALIBRATIO~** Aldicarb Two analyses vere developed; one analysis for high concentrations arid onp for lov concentrations. High Concentration Aldicarb Calibrati0n (Attenuation Range 

### = 0.16) 

### A stock aldicarb standard solution vas prepared by weighing the aldicarb standard into a volumetric flask and diluting to mark with acetonitrile. Solutions ranging in concentration from 0.1 to I mg/mL were prepared by diluting the stock solution volumetrically. Ten microliter volumes of standard solutions were injected into the liquid chromatograph. 

### A linear calibration curve (Figure 

### 1) was obtained when peak areas were plotted versus concentration. The calibration was checked each exposure day. Low Concentration Aldicarb Calibration (Attenuation Range 

## = 0.0025) A stock aldicarb standard solution was prepared by weighing the aldicarb standard into a volumetric flask and diluting to mark with acetonitrile. Solutions ranging in concentration**fro~** 0.001 to 0.010**wg/~:** were prepared by diluting the stock solution volumetrically. Ten microliter volumes of standard solutions were injected into the liquid chromatograpt. A linear calibration curve (Figure 2) was obtained when peak heights were plotted versus concentration. The calibration was checked each exposure day.**::~chlorclLet:'ane** Calibration of the gas chromatograph was achieved by injecting gas standards, which were prepared by syringe injection of test material into ,edlar* gas bags. These standards were prepared using the mathematical :'"clationship:
v C x V h x MW x 298 x P d 

### x 24.45 

### x T 

### x 760 where: V required volume of calibration liQuid"in milliliters (mL) at temperature T in degrees Kelvin 

### (OK) C the desired final calibration concentration in parts per tr.illion (ppm) Vh 

## = volume of container (Liters) MW molecular weight of the calibration liquid

P barometric**pre~sure** in millimeters of mercury (mmHg) d density of calibration liquid in grams per milliliter**(g/~L)** at temperature T 24.45 molecular volume at 298°K and 760 woHg, in**Liter~** T temperature in degrees Kelvin 

### (OK)



### * Registered Trademark, DuPont de Nemours, E. I. 

### &Co.**Rl'p,,~: ~-;-I**  y,**ApPl'l~C~** i :.: I -5-
A linear calibration curve was obtained when areas (integration counts) were plotted versus the gas standard concentrations. Tlte calibration factor (KF) was calculated as: AREA KF 

### = CONCENTRATION The mean KF for all valid injections of standards was used in subsequer.t microprocessor calculations. Methyl Isocyanate Calibration of the gas chromatograph was achieved by dynamically generating gas standards which were prepared using a VICI Metronics standar: rate permeation tube and a VICI Metronics Dynacalibrator 450. The rate 

## 0: methyl isocyanate permeation from a 15 cm permeation tube equilibrated at 50"r. was determined graVimetrically to be 10351 ng/minute. Individual**measureme~ts** were 10099, 10709, and 10246 ng/minute. Gas standards of different Loncentrations were then prepared using the following relationship: C 

## = R x K-Fwhere: C R. F K concentration (ppm, v/v), diffusion rate (ng/min), Air flow rate (mL/min), reciprocal vapor density of material in nl/ng. A linear calibration curve was obtained when peak heights were p10ttec versus the standard concentrations. Peak height measurements extended the linear calitration range (0.9-13 ppm) to the minimum detection limit of 0.9 ppm. Calibration of the gas chromatogr:.?h for the MIe industrial hygiene monitoring system paralleled the calibration methods used for the 

### MIC**cha~:er** atmosphere monitoring system. 

### A 4.0 cm permeation tube equilibrated at,4C=( yielded a 14il ng/minute permeation rate. The calibration standard concentrations ranged from 35 to 1170 ppb. 

### A linear calibraticn curve was obtained when peak areas were plotted versus 

### HIe concentr.ation.**C~~BER** ATMOSPHERE ANALYSIS Aldicarb Two**mi~get** impingers (in series), each containing approximately IS m: acetonitrile, were used to scrub the chamber air samples. Impinger efficiencies were 97% or**grea~er.** A Terracon pump with an in-line critica2 orifice,**ope~ating** at a f10wrate of approximately 700**~L** per minute, was**u5e~** to sample t:;e chamber atmosphere. The sampling system, flowrate was calib::a,:e': using a Singer dry test meter. Samples were collected for 10 to**55,mint~~s;** depending on chamber atmosphere concentration. The teflon samp::'ing**lin,~I0;~5** washed wit:: acetonitrile after each analvsis. The acetonitrile /aldicarb·"·..i.;; solution was analytically transferred to' the front impingersoluti,on;,a.nd" ",:;" included in the analysis.
.RL';l\lrt**:.~-:** j'- :\:';1';":11<\ i :'. : -6-
The least sensitive analysis method (Figure 1) was used for**th~** 46.3**~g** per liter exposure. All other exposure analyses made use of the more sensitive method (Figure 

### 2). The test material used in the 

### 46.3 and 

### 8.7 Ug/L exposures was undiluted; for all other exposures, the test material was diluted ten-fold (w/w) with dichloromethane. The concentration of aldicarb in the chamber atmosphere was calculated as:
C LCC 

### x VI 

### x 1000 

### V

L where: C Aldicarb Chamber Atmosphere Concentration,**~g/L** LCC Aldicarb Chamber Concentration of the impinger solution as det2rm1ned by liquid chromatography, mg/mL VI Impinger Volume, 

### mL VL Volume of chamber air scrubbed, L**~ote:** If the impinger efficiency was less than 100%, both impinger solutions were analyzed aud included in the analysis calculation. The mean aldicarb**conc~ntratlons** (± SD) for the five exposures were 0.82**C~** 0.100), 2.0**(~** 0.15), 6.0 (± 0.61), 8.7 (± 1.38), and 

### 46.3**~g** per liter. See Table 1 for a summary of the analytical data. Dichloromethane DCM was sampled directly by the GC automatic gas sampling system. The chamber atmosphere was analyzed for DCM at least 40 times for each full**4-hou~** exposure period. The mean DCM**conc~ntrations** (± SD) for the five expcsures were 12.7 (± 

### 5.76), 27.7 (± 

### 2.71),54.0 (± 

### 5.19),7.4 (± 1.48), and 49.1 (± 

### 12.97) ppm. These DCH concentrations are equivalent to 43, 

### 94, 183, 25, and 165**~g** per liter. The DCM analytir.al results are summarized in Table 1.**~ethyl** Isocyanate HIC was sampled directly by the GC automatic gas sampling system. The chamber**atmosp~ere** was analyzed for MIC for the 46.3 ug/L aldicarb exposure 

### 5 times and 

### 26 times for the 

### 8.7**~g!L** aldicarb exposure. 

### No MIC was· detected. The minimum detection limit was 

### 0.9 ppm. For the other three exposures, the MIC analyses were discontinued since the further dilution of the test material would decrease HIe concentrations by another factor 

### of ten.



# -- l

. - Date



### lftPC/rkk!0894B-4 10-14-85Rt.:p.',rt :.:;.:-: 'J1 Appt"n';! ;-; J -7-
Table 1 Analytical Results Summary
A. 0.82**~g/L** Aldicarb Exposure Aldicarb Concentration, H&/L Dichloromethane Concentration,**pp~** 0.68, 0.90, 0.88, 0.80 Mean: 0.82 SD: 0.100
B. 2.0**~g/L** Aldicarb Exposure Mean: SD: Samples:

Range: 12.7 5.76 44 3 to 43

A1dicarb Concentration,**~g/L** Dichloromethane Concentration,**pp~** 1.8, 2.1, 2.1, 2.1 Mean: 2.0 SD: 0.15
C. 6.0**~g/L** Aldicarb Exposure Mean: SO: Samples:

Range: 27.7 2.71 44 22 to 34

Aldicarb Concentration,**~g/L** Oichloromethane Concentration,**P?~** 5.8, 6.9, 6.3, 5.9, 5.3 Mean: 6.0 SD: 0.61
D. 8.7 ug/L Aldicarb Exposure Mean: SO: Samples:

Range: 54.0 5.19 40 44 to 64

Aldicarb Concentration,**~g/L** Dichloromethane Concentration, F?= 9.9, 9.4, 9.8, 8.7, 7.9, 6.3 Mean: 8.7 SD: 1.38
E. 46.3**~g/L** Aldicarb Exposure Mean: SD: Samples:

Range: 7.4 1.48 44 4.2 to 11.4

Aldicarb Concentration, Ug/L Dichloromethane Concentration,
Mear.: SD: 46.3 46.3 Mean: SD: Samples:

Range: '
..9.1 12.97 9 23 to 65 WPC/rkk/0694B-3 10-15-85

# •

2258.
2iHlfl.

175iL**1::5~.**



## e. Eo. I 0. 2 Rt.·;
...·,rl**':'K~** l ),., ApI"'IHI i :.; I -8- Figure 1 Aldicarb Calibration Curve



# ./ /,**.....I--~----I---.---....,...i** 

### ---'---,' 

## e. S £1.7**LL·~** 

### MG/ML**ALDICRRB~~**R"'l'.'rl :K-\ 36 Ajlpl·nd i :.: I
-9- Figure 2 Ald1carb Calibration Curve

2. r--r---,r-----.---.j---.----y-,---r-,---"r----"T-...,--,I: .' 4. 6.1 

### 8.11'iL1 1.88.

90.
p 

## 8e. E R K 78. H E 

## 6e. 1 G H T SB.

M 4B. rl 3B.
2B.
1&.

0. 

### B.APPE:\DE 2 Individual Animal Pathology Data Aldicarb Solution (in DCM) LCSO Aerosol Acute Inhalation Toxicity Test (5.0 Pages) Report 48-136

# •

Test Name: Acute**Acro~()1** Jnh.Jl.Jt ion Tn:-:icit·
.. (I.l'>\" Path. P;lft' "1 Test 1'0.: 14 PAT H 0 LOG y**RL'i~l)rt** :.;-'-1 :,' APPL'lld i ;.; .'
Sample No. : 48-121 A Species: Rat Animal />;0.: Sample Name: Aldicarb**DC~l** Strain:**Z~l/S-[)** Treatment Level: 0.0463 mg/liter Sex: Fate: -X-D E EM Kodachrome: Gross Female Date of Death:**~1i** cro Age:**8-~ ~cc~e**  7/12/85 Clinical Histor, 

# '-

= - Gross**Findin~s** 

## I Date**T::':',_€~** 

### - Red stained, bilateral. i /12/85 : :**;J_~ .~_\:**  

## -**Ri~ht** 

## - Corneal opacity. Or
..'an


### ....**:~-L..** 1£)

- Liver Lungs 

# -**~kttled** light and dark pink, all lobes. K:cne\'s S::leen ; Right kic:-.e\' 

## - 10 x 5 IlL':: clear fluid filled cyst on Heart : cortical suriace; inadvertantly cut long. Brain



## - Severe hydronephrosis, bilateral. Pitui'tar,\' Bronchi :i.crenals Xasal ca\'it\', Trachea, Liver, and 

# - No gross GO!lads ' lesions. 

## I Eo='; I
....eight·

D -**Sponta:-.e;)~s**

## :a:::. P:lf.e /12 PAT H 0 LOG Y

-------- R

...p"rt :'.<;-1 :I" APlk'''cJ i :.: ' 14 Test Name: Acute Aerosol**Inh~]ati0n** Tnxicitv (LCSUJ '=-"-"-"-'--- Sa:::?le No.: 48-121 A Species: Rat Animal t-;o.: 8':,-167E3 Sa=?le Name: Aldicarb 

### DeM 7reatment Level: 0.0463 mg/liter Sex: Strain: Female ZM/S-D Age: fate: 

### -lLD E P.1**Kc~achrome:** Gross Date of Death:**~li** cI'D 711 '2/85 Clinical Ristor,

.....

I

# I I ;', Gross findings I I 

## I Date 

## I I Ti:::e 1 

### - Lenticular opaci:y, red stained, bilateral. 7/12185 

## - I I 11: 36 ;...:..: i OrDan I '.-;L_ (c-) 

### i 

# - Mottled light and dark red, all lobes. Liver I 1 

# I :;::'5a 1 cavity. Trachea, Liver, Kidnevs, and Bronchi Kidne\"s i



# - Ko 5nleen I ! gross lesions. Heart I 

## I Brain 

### i 1 Pituhar\" ! I! Adrenals , I**~cs** I



### -- I Boc'\; ;"·eight I :Path.**P~1r,"** " 3 R\.'!)l'rt ':',",- J j1i Appt::h'Ji:.: '.! Test No.: Sample No.: 14 48-121 A Test Name: Acute Aerosol ]n11Lllatjon**TQ~;icit\'** r!.C:'(11 Species: Rat Animal };o.: 8:;-1>';".:.

Date of Death: !'1icro Sample Name: Aldicarh DCM Treatment Level: 0,046) mi:/liter Sex: F'ate: -X.....D E EM Kodachrome: Gross Strain: Female 

### ZM/s-J)

____---'7.1l.'2.<-I
..... 

## c_,

_ Clinical Historv

Gross Findin s Right

- Corneal opacity.

- Left

- Red stained. Cervica! lvmph nodes

- Enlarged 

### 3X normal. Lungs

- Mottled light and dark red, all lobes.**~as21** cavity, Trachea. Liver, Kidneys, and Bronchi

-**~o** gross lesions.**1====;~D~:!:e'-====~==iTb'.::..** ,:::_e""._;-,.=,=,,! 711'2/85 L::u :\.." Or an Liver Kidnevs S leen Heart Brain Pituitan' Adnmals Gonads Bod
...· "'ei£::'t·Acute Aerosol InhalatiC'D Toxicit·,. (LC:"(I) Path. r.lf.<' rest**~o.:** Sample No.: sample Name: 14 48-121 A

Aldicarb DCH Test Name: Species: Rat Strain:**R('r~'rt** !.;"';-1)1**;\pj1~·n** ...; i :.: ...:
Animal No. : ZH/S-D rreatment Level: O.0463mg/liter Sex: Female Age: 8-9 Weeks Fate: XD E EM Kodachrome: Gross Date of Death:**~licro** 7/l2!8S

I

# r Clinical Historv

Gross Findin s ---------l Tin!? Date 

# 'El 7Il?/P,;**Il...;~.-r···** Own I**~l....** (£) Liver Kidnevs , Spleen Heart Brain 

### I PituitarY Adrenals Gonads 

### == 

### Boe,**h'ei.>:~t** . I

"

'.

:**~a5al** ca,it\", Trachea, Liver, Kidneys, Lungs, and Bronchi

- No gross**le~.** - Lenticular opacity, bilateral.Path. P:lfl'
.. " PAT H 0 LOG Y R E COR D



### --------- ------ Rt..'j'\

# j" t _;"'i-:**~r\** '\1'1"'11<1 i:-: " Test Name: Acute Aerosol Inhalation To>:icin (Lt.:"!' Species: Rat Animal No.:**8'J-;i)7b~** Treatment Level: 0.0463 mg/liter Test**~o.:** Sample No. : Sample Name: 14 48-121 A

Aldicarb DC1'l Sex: Strain: Female 

### ZM/S-D Age: 8-9 I..'t:d:s Fate: -.lLD 

### E EM Kodachrome: Gross Date of Death:**~li** cro 7/1**)/~~** Clinical Historv

Gross Findin s
Or an 7 12/8)**~asal** cavitv, Trachea, Liver, Kidnevs, Lungs, and Bronchi**-~L~i~v~e~r~ ~~**  gross lesions.**Kid~Evs** S lee" - Lenticular opacity, red stained, bilateral.

Heart Brain Pituitary Acirenals Gonads Bod
... \,eight !Report 48-136 Appendix 2

Strain:
.....**~Z~M~/~S~-~D~** __ FemaIe Age:

_ .......:.7....:!!.w.=e.=e.!::k:.=s

.....__ Date of Death:**~7~/~2~5L/~B~5** ___ Micro R E COR D



### ------

Sex:**--~=~----** Gross Test Name: Acute Aerosol Inhalation Toxicity (LeSO) Species: Rat Animal No.:· 85-17083

Kodachrome: Path. PaCe 11 6 Test No.:

__**~14,,--_**

Fate: -X...D Sample No.: 48-121 B Sample Name: Aldicarb DCM Treatment Level; 0.0087 mg/liter 

### E EM Clinical History

Gross Findings Da.te Time

- Lenticular opacity. red stained, bilateral. 3: 11**P~1** Lungs

- Dark pink. all lobes. Liller Kidnevs**~asal** cavity. Trachea, Liver, Kidneys, and Bronchi

- No. gross lesions. Spleen Heart Brain Pituitarv Adrenals Gonads Body WeightReport !oS-I3" Appendix 2

7/25/85 Age:

__-'7'---'w-'f::..:e:..:k.:.;s"--

__ ZM/S-D Strain: Sex:

__**~F,""e~m~a~l,",e=--** _ Date of Death: l'licro Test Name: Acute Aerosol Inhalation Toxicity (Le50) Species: Rat Animal No.: 85-17084



### E EM Kodachrome: Gross Sample Name: Aldicarb DeM Treatme "Level: 0.0087 mg/liter Path. Pace I! 7 Test No.: 14 Sample No.:

__**~4~8-~12~1~~~** __

Fate: --!-D Clinical History 

## 1 I

Gross Findings Date Time Eyes

- Lenticular opacity, red stained, bilateral. 7/25/85 2:59**P~l** Orean \\'r**(.,~** Perinasal area 

## - Red encrustation Liver Kidneys I.ungs

- Dark pink, all lobes. Spleen I Heart Kidnevs

- Mild hydronephrosis, bilateral. Brain Pituitarv 

### I Trachea, Liver, and Bronchi 

# - No gross lesions. Adrenals Gonads Bod\'**\\'ei~htl** 

## .

,:':' Prosector:**H~~Stae~,** , rod

D Spontaneous Death**:a~h.** Page II 8 Rerh'''rt !.K-l**~tl** J\ppt,.'nd i :.: RECORD



### ------ ,e"t No. : Sat::ple No.: 14 48-121 B Test Name: Acute Aerosol Inhalation Toxicity (LC5(Jj Species: Rat Animal No.: 85-17(J!,,,
0.0087 mg(liter Sex: Female Age: 7**wt:t::k~**

E EM .Date of Death: 7(25/85 Kodachrome: Gross Micro Sa=?le Name: Aldicarb'DCM Treatment Level: Fate: 

### -LD Strain:**Z.~l(S-D**

Clinical Historv --------- ..-=- I :

, : I I

# !
- Gross Findings j 

# 1 I Date T-i.J.le, \ Lenticular opacity, stained, bilateral. 7/25(85 { 3:05**?~:** ! 

## - red 

## I OrDan i \.:.- (-)**l"J;1~** S 

# - Dark pink, all lClbes. Liver I 

# I Kidnevs i :\asal cavity, Trachea, Liver, Kidneys, and iSronchi 

## - :-"0 Spleen i 

# I gross lesions. Heart i Brain I 

### I Pituitar". ! 

# I Adrenals i hOMads \ Bod\' '\o,'ei,"ht I 

# I

I

# I I 

# I

,

Pre-sector: 

### .... ; ;',.'>;<, D Spontaneous Death Euthanized

## E~:, .';'Euthad'z.?d

## ";'rro=::,~:-;~:';~~:" • \':::'f-iJ " '\".,'

.

## ,.§a:;."i.m~~

# •

Report 48-136 Appendix 2 Path. Pace 11 9 R E COR D

------

E -Euthanized 

### D

## =.spontaneous Death Test No. : 14 Test Name: Acute Aerosol Inhalation Toxicity (LCSO)


# - Sample No. : 48-121 B Species: Rat Animal No. : 85-17086 

### .. 

# - Sample Name: Aldical"b DCM Strain: ZM/S-D 

## , Treatment Level: 0.0087 mg/liter Sex: Female Age: 7 week.s Fate: ..L,D E EM Date of Death: 7/25/85 Kodachrome: Gross Micro 

### - 

## ., C1inica1 Historv J 

# , i i 

### I



### = Gross Findings Date Time ,.

Eyes 

## - Lenticular opacity, bilateral 7/25/85 2:53**P~** OrOaT> 1-1t- (p) '. Lungs 

### - Dark pink, all lobes. Liver Kidnevs Nasal cavitv, Trachea, Liver, Kidneys, and ,Bronchi


## - No Soleen gross lesions. Heart Brain PituitarY Adrenals Gonads I Bodv Wei2ht

; 

## , :



### , ;

,.

, ,

" 

# ;"1 -..... 

### , "j "• Prosector:
.**·-::~\.~5 !::t?~-l**  

## .**-~. ',,~** **.~~** :,.1. .' .. ni,d';'1

# H

-) .•_'. c,L"

# •

Report 48-136 Appendix 2

7 ,:"leeks Age: ZM/S-D Strain: Female Sex; Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species; Rat Animal No.: 85-17087 Path.**Pa~e** 1110 Test No.: 14. Sample No.:**_.:::.;48::..-~1:.::2:.:.1...;B=--** _ Sample Name; Aldicarb DCM Treatment Level: 0.0087 mg/liter Fate: 

## -1LD E _EM Kodachrome: Gross Date of Death: Micro 7/25/85

I Clinical Historv



### Gross Find1n2s D"te Time



## - Red encrustation, bilateral.**7'~** 2'47 py 

## , 

# - Right 

### - Lenticular opacity. Draa"" \-I.- 7.;) Liver Lungs 

# - Dark pink all lobes. Kidnevs Spleen Kasal cavity. Trachea, Liver. Kidneys, and Bronchi 

# - No Heart gross lesions. Brain Pituita!"\.' Adrenals Gonads Body We12ht

'Prosector:**H~fStc:t?'2** 

# !**-~** 

## !

••\.J 

### D = Spontaneous Death**-----,----,~._-----....;------,~.:....;.,-....;-_--::.~~~..;..,-:.'_.-:-::.:--_.-:-..,...;..:.-** '.:**.~.t** -, ,',',-:" -:"",'", '"':,',,,': ',.-' :--,",- ':. " :: ,,:,:.'

### Euth~,~i:ed;whe.rl'incir:.~~~t;,

;. ." ,'" "'6f77. ;.....; '

...-',"••,....,'•..',

.. . " .,: ,:'':'?,::'

.Clinical History ... (LC50) ,',:

10-1 I 811/85 Report 48-135 Appendix 2

Animal No.: ZM/S-D Acute Aerosol Inhalation Toxicity

Sex:**_--,F'-.5e:JJm~a!."!l,",,e,--** _ Date of Death:**~licro** Species:

__....!.R"'a
.... t'--

_ Strain:

Gross Test Name:

Kod3chrome: Path. Pane (I 11 Test No. : 14 Sample No. : 48-121 B Sample Name: Aldicarb DCM Treatment Level: Q.DQ6D**m~/11ter** 

### E EM Fate: -lLD

.j



## .-1

# !

: i i· Gross Findings .;. Eyes

- Red encrustation, lenticular opacity, bilateral. Date RillS:; , Time _! 9: 47 A}! Cervical lymph nodes

- Enlarged 

### 3X normal. Lungs

- Mottled light and dark red, all lobes. Orl<an Liver Kidne"s Spleen Nasal cavity, Trachea, Liver. Kidneys, and Bronchi

-**~o** gross lesions. Heart Brain Pituitarv .!' Adrenals Gonads :..: Bodv Weight !.'

D Spontaneous Death '-'DIPath. Pace II 12 Test No. :

__..:1,--4:-_ Sample No.: 48-121 B Report 48-136 Appendix 2
Test Name: Acute Aerosol Inhalation Toxicity 

## (Leso) Species: Rat Animal No.: 85-17172 Sample Name:**_..:.A~l~d:,.=i:.::c:.::a~r~b~D..:::C.:.:M** _ Strain:**_~Z.:.:M!,..;/S~-....:D:..-** _ Treatment Level: 0.0060 mgiliter Sex:

__..::F..=e:,::m:,:::a:,.=l:.::e_____ Age: 10-11 '>leeks Fate: ..JLD E

__ EM Date of Death:**7;..!/..:3::.;1'-'-/..::8~5** _ Kodachrome: Gross Micro Clinical Historv

Gross**Findin~s** D"te TimlL 7/<I/Rt; 

## ,."r; 1'" J.eft eye - Lenticular opacity. Or<>an \\'r (12) Trachea

- Thick Il1.lCOUS present. Liver 

## '. Kidnevs I !,asal cavity, Liver, Kidneys. Bronchi. and Lungs

- No Spleen Heart I lesions. gross Brain I Pituitary \ Adrenals I Gonads I Bodv Wei2htl



# \ Prosector: ,H.'15t",,:: ."1

. , ..,c(

D Spontaneous Death ',' -. EIJ'than

# ized~~en:**'~C'~.i~\~~~:~'\·** ".'"**'~/** '.,

..: ..**,.-~':~,'-><: ~'.,,>i._·c:,**  .**.'._,~:**.• '

# >:~';;;i);,i,:;,:'••

# >~;:~;j':.•

# :':~;~1J,s;l~~~Report 48-136 Appendix 2

Strain:**_-=Z~M'.L/~S.::;-D~** --:-

__• RECORD



### ------ Test Name: Acute Aerosol Inhalation Toxicity (LC10) Species: Rat Animal No.: 85-17173 

### PAT H 0 LOG 

### Y



## --------- Sample No.:

__**~4~8~-~1~2~1~B~** ___ Sample Name: Aldicarb DCM Path. PaE\e (I 13 Test No.: 14

Treatment Level: 0.0060 mg/liter Sex:**~F~e~m~a~l~e** __ Fate: --K...D E EM Date of Death: Kodachrome: Gross Micro Age: 10-11 weeks 8/1/85 Clinical Ristor



## I# I=============~==========l·.i

Gross Findin s 

### ii Eyes

- Red encrustation, bilateral. Cervical lymph nodes

- Dark red. Lungs

- Mottled light and dark red. all lobes. Nasal cavitv,**Tra~hea,** Liver, Kidneys, and Bronchi

- No gross lesions. S leen Heart Brain Pituitarv Adrenals I"

# ~~g~n~/g:.**d~~~e=1=.**=h=t=i======9.

# , 'fl**~(;,** 

### ,. 

# tReport 48-136 Appendix 2

ZM/S-D Strain: Sex: _--:F:..;e:.;m:::;a:..l:..e=-_____ Age:

__:.12::.-....:.1:.3_w::.::..E:E:::.k:;.::.s_ Date of Death: 8_/_1_4_/_8_5

_ Niero Gross Test Name: Acute Aerosol Inhalation Toxicity (LCSO) Species: Rat Animal No.: 85-17171

Kodachrome: sample No.: 48-121 B Sample Name: Aldicarb in DCM Treatment Level: 0.0060 rng/liter Fate: _D X E

__EM Path. Paf,e I! 14 Test No.: 14

Clinical Historv

Gross Findine:s Date Time Nasal cavity. Trachea. Liver, Kidneys. Bronchi, and fl/I/,/flr:. ll..:;1'\ A" Lungs

- No gross lesions. Oroan 

## "'to ,( c) Liver Kidnevs Spleen Heart Brain Pituitarv Adrenals Gonads Bodv WeiQ:ht

I I Prosector: \ H}!Sto?d 

### ! 'i'md: D**Sponta~eous** Death**.~'** ", ' ';**!_~_ 1"':~**  ,

### Eutnah~~~,d**wll~n~.~:M;~.~~:::'**Path. Pace (I 15 Test No.: Sample No.: 

### 14 48-121 B Report 

### 48-136 Appendix 2 Test Name: Acute Aerosol Inhalation Toxicity (LC50)

_ Species: Rat Animal No.: 85-17174,'- 

# - Sample Name: Aldicarb in DCM Treatment Level: 0.0060 mg/liter Sex: Fate: D XE EM Kodachrome: Gross Strain: ZM/s-D Female Age: Date of Death: Micro 

### 12-13 weeks 8/14/85 Clinical Histor'

Gross Findin s Nasal cavity. Trachea, Liver, Kidneys. Bronchi. and Lungs

-**~o** gross lesions.



### D =Report 48-136 Appendix :2



### sample Name: Aldicarb 

### in DCM Treatment Level: 0.0020 m2/}lter Perna1e Age: _-'1
.... 2...;- 1
.... 3'--"'w
.... wJi.e".ks,,--_ Date 0 f Death: -.loL8L Il3'/"/u 8 "-51.-.

__**·~ticro** Sex: Test Name; Acute Aerosol Inhalation Toxicity (LC50j Species: Rat Animal No.: 85-17103 Strain: ZM/S-D
-X...E EM Kodachrome: Gross [I 16 path. Par,e 

### rest No.:

__**....a.:14~_** sample No.;**_--=4<.1<8~-;.l.1**.. 2..1J._B

_
Fate;

__ D Clinical Historv



### Gross F::'ndings
Nasal cavitv, Trachea, Liver, Kidneys, Bronchi, and Lungs

-**~o** gross lesions:- Date Or""'" Liver Ti!:}.c. Q. Ol. A"I
Kidnevs Spleen Heart Brain Pituitarv Adrenals Gonads Bodv**Wei~htl**

I'rosettor:
.... H"Src';-Repo'rt 48-136 Appendix 2



# - 85-171 

### 04 ·r 

# -

# . Animal No.: ZM{S D Bat Strain: Mute Aerosol Inhalatjon Tpxjcity (1'[50) Species: Test Name: 14 48-121 B AJdicarh in DCM Path
.. Par;e (I 17 Test No.: Sample No.: Sample Name: Sex:

__.... F"'p;.um""auJ"'p'--

_ Treatment 

### Level: Fate: D Q QQ?Q mgIJ iter EM Kodachrome: 

### Gross Date of Death: Hicro Age: 12-13 »'peks

# - 8/13185 Clinical Historv


### , '1



### Gross Findings Date Nasal caVity. Trachea, Liver, Kidneys, Bronchi. and Lungs

-**~o** gross lesions. 8/13/85 Orl<!an Liver 9:09 Al-I
Kidne
.... ·s Spleen Heart Brain Pituitarv 

### Adrenals Gonads I Bod
..... t<eil:(hti

D Spontaneous-DeathReport 48-'136 Appendix 

## 2 Path. Par.f..' I' 18

8113/85 12·-,13 weeks Age: Date of Death: Micro Female Sex: Gross Test Name: AcUte Auoso1 inhalation Toxicity (Le50) Species: Rat :. Animal No.: 85:17105 Strain: _'7ZM

......

! S

...

---'D"--

_

Kodachrome: 14 Test No. : Sample No.: 48-121 B Sample Name: Aldicarb in DCM Treatment Level: 0 0020 mg/liter Fate:

__ D J-E EM Clinical Historv --I

Gross Findings
Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and

Lungs

-**~o** gross lesions.

# -, 85-17106
12-13 weeks 8/13/85 Age: Animal No.: ZM/S-D Rat

Date of Death: IHcro Strain: Female Species: Aldicarb in DCM 48-121 

### B (I 19 14 Report 48-136 Appendix 2

Sample Name: Path. PaBe Test No.: Sample No.:
Treatment Level: 0.0020 mg/liter Sex:



# ---------- Fate: D X E EM Kodachrome: Gross Clinical Ristor

Gross Findin s Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and Lungs

- No gross lesions. Kidnevs S leen Heart Brain Pituitar Adrenals Gonads I Body Wei ht



### ,', ",I, ,:,::-

.**-~.-~:.** ;



# ,.I,Sample No.: 48-121 B Species: Rat SalIIple Name: Aldicarb in DCM St.rain: Treatment Level: 0.0020 mg/liter Sex: Female Fate:

__ D 

### J.....E EM Date of Death: Kodachrome: Gross !'licro Path. Par,e (/ 20 Test No.: 14 Test Name: Report 48-136 Appendix 2 R E COR D



### ------ Acute Aerosol Inhalation Toxicitv (LC50) Animal No.: 85-17107 ZM/s-D Age: 12-13 weeks 8/13/85



## - I Clinical Ristorv

Gross Findin2s 

## I Date**Til!1e~** Cervical lymph node

- Enlarged 3.5X normal. 8/13/85 9: 14 A.'l Oro:>" \it (c) Nasal Tt"Bchea, Liver, Kidneys, Bronchi, and Liver 

### I cavity, Kidnevs I Lungs

- No lesions. gross Snleen I Heart ! Brain 

### I Pituitary I Adrenals i Gonads I Bod\' Weil!htl

.-,.-**.~**..Report 48-136 " I Appendix 2

__--<..:14::....._ Test Nanle: Acute Aerosol Inhalation Toxicity (LC50)

...... 48-121 B Species: Rat Animal No.: 85-17098 ." 

# - Path. Pace j,2l Test No. : Sample No.:



# - 9-10 weeks ",. 

# -

.. 8112/85**"~** Sample Name: Aldicarb in DCM Strain:
.... Z
..... M!..L.I
.... Se--"'D ..:::."\ Treatment Level: 0.00082 mg/liter Sex:

__--IF-'ie<Jjm
... a .... l
....

e'--___ Age: Fate: D.-X..E EM Date of Death: Kodachrome: Gross Micro 

# --,., 1- ---=C.=.I.=.i::..:n-=.ic:=;a:=;I::......:}c:,:ll:.:· s::..:t:.:o:.:r:...ly'---

Gross Findings Right kidney

- Marked hydronephrosis. Date R/]7/Re; Oraan Time 

# :--l g. 1" A\I
. Nasal cavitv, Trachea, Liver, Left kidney, Bronchi, and lungs

- No gross lesions. Liver Kidnevs Spleen I Heart Brain .Pituitarv 

### I Adrenals Gonads I Bod"**~eight**

Prosector:

D Spontaneous Death E
... Euthanized EH Euthanized .:he:l. r..::,db:':::!d' 6/.ii.Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Anima1 No.: 85-17099 14 48-121 B

8/12/85 Report 48-136 Appendi:-: 2

Female Strain:**-=Z::.:.M:.!./~S~-~D** ,..._----- Age:

__**9:..-_1:..;O::......:w:..::e:..::e~k:.:s:-..-** Date of Death: Micro R E COR D

------

0.00082 rug/liter Sex:**~E** EM Kodachrome: Gross Treatment Level: Fate:

__ D Path. Pace (I 22 Test No. : Sample No••: Sample Name: _-,A2:.:l**d~i::c~a,=-,r~b~·** ...**i!:.-'n~D:.::C::..M:....-** _



## me I Clinical Historv

Gross**Findin~s** 

## I Date Ti!:!!' R/1., IRO; Q.f\-q A'1 Right kidney

- Marked hydronephrosis. Or"",,.,

### "'~ (£' ) Trachea, Liver, Left kidne\', Bronchi, Liver Nasal cavitv, and Kidnevs I lungs

- No gross lesions. Spleen I Heart Brain I 

### I Pituitarv I Adrenals 

### i Gon:lds Bod\' "'eight 

### ! 

# I

I 

# i I I I

# I

I I I 

## " 

# . ,. 

# I**----------------------~-----_** ..

# _--------- D Spontaneous Death E ., Euthanized Euthan'ized**~henmcri~~=:** 

## :0.':.::(:
..··Acute Aerosol Inhalation Toxicity "'Vf!" Report 48-13,?" Appendix 2
.**~:**.


### ......**.,·:~l\** 

# .. (Le50) ,**;~ij: 85-17100'~~** 

8/12/85
.'tf; Age: Animal No.: ZM/S-D Female Date of Death: Micro Species: R:.:a=-=..t

_ Strain:

Clinical History Test Name:

0.00082 !ltg/liter Sex:**~E** EM Kodachrome: Gross 14
Aldicarb in DeM 

### 48-121 B

Fate: D Path. Page 1 123 Test No.: Sample No.: Sample Name: Treatment Level:

.

# ..,... :?l

••• 1

Gross Findings
Kasal cavitv. Trachea. Liver. Kidneys, Bronchi. and Lungs

- No gross**lesi~** Date 8/12/85 O"oan Liver Time 

## '."1 8: 59 &'1
. .! Wt (I!) Kidnevs Spleen .1 Heart Brain Pituitary Adrenals**~G~o~n~a~d:'1s;===:=9====="~** Bod
...·- I-I'eight

### I i.

D Spontaneous Death E = Euthanized ProsectOr: .H}lSr'"el 

### --1

...' ". m,d<.•

### j'.

# E~I = Euthanized

# '~1-H~'n~o;ib~/9-10 WE:E:ks 8/12/85 Age: ZM/S-D Female Strain:
Date of Death: Micro Sex: Gross Test Name:**Acut~** Aerosol Inhalation Toxicity 

### (LeSO) Species: Rat Animal No.: 85-17101 Report 48-136 Appendix 2 Path. Pace 1./24 Test 

### No.: 14 sample No.: 48-121 B sample Name: Aldicarb in DCM Treatment Level: 

### 0.00082 mg/11ter Fate:

__ D -LE

__EM Kodachrome: 1 I Clinica Historv 

# I

- Gross Findings Date Til:le**8/12/R~** q·o.!, AV

Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and Or2an t-;'t

. (r. ) Lungs 

## - No gross**lesi~** Liver Kidnevs Spleen I Heart Brain Pituitarv Adrenals I Gonads I 

## , Bodv Weight I



# -

"

, , 

# I ,.,: l i' Prosector:**H~··':::'-** - i

, rnd i

.,,1 D •**'Spontaneous~Death** E "" Euthani'zedTest Name:**Ac~te** Aerosol Inhalation Toxicity (Le50) Species: Rat Animal No.: 85-17102 48-121 B Aldicarb in DCM 

# ,

.i J .j I _,f 

### -.1.;

.,..



### :.. --;l: 9-10**Io;eeks....:..~·:** .';' 8/12/85 .•.. Report 48-136 Appendix 2

Age: ZM/S-D Strain: Sex:**~F~e:.!!.m~a,-:!:l,",e,,-** _ Date of Death: Micro Gross PAT H 0 LOG Y

EM Kodachrome: 0.00082 mg/liter 

### -XE 14

Treatment Level: Fate: D Path. PaGe (I 25 Test No. : Sample No.: Sample Name:

".. .'.\

# -

. .. E'c Euthanized Spontaneous Death D Clinical History

Gross Findings 

## I Date Time

__ Trachea, 8/12/85**8~!.1** 101 Nasal cavity, Liver, Kidneys, Bronchi, and OrO""**~1'** (01 Lungs

- No gross lesions. liver Kidneys I 'Spleen Heart Brilin Pituitilr
...· Adrenals I Gonads I Bodv I.'eight

### I

i'

Prosector:
.**H~lSt,~el:**



### .. .crod:·: 

### }.:, .,', ':-., ',,' ,',;
..Report 48-136 Appendix 2 Path. Par.e (126

Clinical Historv Sex:

__-lMa-.,lu e :....-_____ Age: 7-8 weeks Date of Death:**7..:./_]..;::2~/..:.8~5** _ !oUcro Gross Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-1676"- Strain: 

### ZM/s-D

Kodachrome: Sample No.:**~4:il80!.:-:..lw2** ...

1I-'AI..-

_ SalIlple .Name: Aldicarb DCM Treatment Level: 0.0463**m~/liter** Fate: -2..D E EM 1est No.:**1~4:-..**__



# -- I

Gross Findin2s

Eyes

- Red encrustation, bilateral.

- Left

- Lenticular opacity.

- Right

- Corneal opacity. Date .,/1?/fl<; 

### 0,.°"" Liver Time 17· 1 R PV Right kidney

- Moderate hydronephrosis. .Kidnevs I 5nleen I Lungs

- Dark pink. Heart i Brain Kasal cavity, Trachea, Liver, Left kidney, and Bronchi

- Ko gross lesions. Pituitary 

### I Adrenals i Gonads i Bod\' Weight I

E., EuthanizedTest Name: Acute Aerosol Inhalation Toxicity (LCSO) Species: Rat Animal No.: 85-16765 48-121 A Strain:**_...;Z::;M~/:...:S::..-..::.D:...-** _ Report 48-136 Appendix 2 R E COR D

------ Sample No. : SampIe Name:**_~A:=l:.d..:i:.c:.a:=r:.b~D..:C::;M=--** _ Path. Pase I' 27 Test No.: 14

Treatment Level: 0.0463 mg/liter Age:**_--:.7_-...:8:......;;w..:e..:e~k:.:s~_** Sex:

__.:.:Ma=l:.e

_ Date of Death: -=-7**:...11:.:2:.:/..::8:.::5~** _ folicro 

### E EM Kodachrome: Gross Fate: ..1L-D Clinical History

Gross Findings Eyes

- Right

- Lenticular opacity.

- Left

- Corneal opacity. Date Oroan Time 12:'2 PH Lungs

- Dark pink, all lobes. Liver Kidnevs Nasal cavity, Trachea, Liver, Kidneys, and Bronchi

- No gross lesions. Spleen Heart Brain Pituitary Adrenals Gonads Body WeilZht

D Spontaneous Death 

### .E" Euthanized :p

## ro~,e~t~~/ ••

## :~~l~t,~~,i,.i~~>~i:**"~:'"** '. :':.>;,-.{::":, I

# ",,~'\**~'. ~;~ft~~;,~~:f,~~~t~~r~~** Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-16766 Report 48-136 Appendix 2 Path.**Pa~e** 1/26 rest No.: 14 Sample No.: _.::4.:::8--.:;.12=..1=-.:A"------ Age:**_--'7'--~B::........:w:.:e:.:e:;.:k~s::...._** ZM/S-D Strain: Sex:

__**.:.M~a~l:=e,--** _ Date of Death:**----'7"-t/~1:..;;2"-/..!:!8~5** _**~1icro** 

### E _EM Kodachrome: Gross Fate: 

### -L D sample Name: Aldicarb DCM rreatment Level: 0.0463 mg/liter

Clinical Historv

Gross Findin2s Eyes

- Red stained, bilateral. Date**Tim~.** 7/12/85 12:05**p~** OrC7a'" \\It (I') Nasal cavity, Trachea, Live£, Kidneys. Lungs. and**Bronchi-r.=L~i~v~e~r~b===~~~=d~==~**

No gross lesions. Kidnevs Spleen Heart Brain Pituitarv Adrenals Gonads I Bodv**~ei12htl**

D

# ,,= SpantarleOus
.Death'

# •

Report 48-13& Appendix 2 R E COR D



### ------ Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-16767 Path. Pace (I 29 Test No.: 14 Sample No.:**_..::4~8:....-...:1..::2...:1--.:.:A** _ Sample Name:**_..:.A.::l~d::.:i:.:c:.:a::.:r:.:b:......:D:;,;C::.:M..:.....** _ Treatment Level: 0.0463 mg/liter 7/l2//j5 l.ge:

__**7~-...;l:l~w~e::..:(.:,;J,.~· ,,~.** __ ZM/S-D

Micro Strain: Sex:

__..:M:a:..:l:;.:e=--

_ Date of Death:

_ EM Kodachrome: Gross E Fate: 

### -LD Clinical History

Gross Findings Date Time Eyes

- Lenticular opacity, red encrustation, bilateral. 7712785 On'an 11:51 AH Lungs

- Mottled light and dark pink, all lobes. Liver Kidnevs Nasal cavity, Trachea, Liver, Kidneys, and Bronchi

- No gross lesions. Spleen Heart Brain Pituitarv Adrenals Gonads Bodv Wei!!.ht

,L

I'

I"path. Pace It30 R E COR D

------ Report 48-136 Appendix 2 Test No.: 1..,4=-_ Sample No.: 48-121 A Test Name: Acute Aerosol InhalatioD Toxicity (LC50) Species: Rat Animal No.: 85-16768
Male Age:

__**~7~-~8~'i~e~.~k~5** _ Dateof Death: _7L.ft-l

.... 2ufuBJ..5L-

_ Micro Sample Name: Aldicub DeM Treatment Level: 0.0463 mil/liter Sex: Fate: ..lL..D E _EM Kodachrome: Gross Strain: ZM/S-D

Clinical Ristor'

Gross Findin s

- Lenticular opacity, bilateral. Nasal cavity. Trachea. Liver, Kidneys, Lungs. and Bronchi

Ko gross lesions.i

,. 1

ht -or Sex:

__..:.M::;a""l..."eO--_____ Age:

__**....I6l:.-:....7~wt.lie:J;:e:JSk..:ls'_·'** Date 0f Death: 71..J,...{ Z'-SJ..{u8:l.5l.-- 

### ,l Micro Gross Clinical Histor Test Name: Acyte Aerosol Inhalation Toxicity CLC50) Species: Rat Animal No.: 85-17036 Strain: ZM/S-D 0.0087 mg/liter _E _EM Kodachrome:

Eyes

- Lenticular opacity, red stained, bilateral. Report 48-136 Appendix 2

Right kidney

- Mild hydronephrosis.
Trachea, Liver, Left kidney, and Bronchi

- No gross lesions-.

-- Perinasal area - Red stained.
Lungs

- Dark pink, all lobes. Path. Paee (131

Fate:**_~** . 

### ..D Test No.:**1~4;:......_** Sample No.:**_-=4!.!:8~-~1-=2~1-,B~** _ Sample Name:**_~Al:l~d!.!i,-!:c:..!:a!.!r:.Bb!......!:!D~Cc!:!M,--** _ Treatment Level: 

# "I t**r============~~========~1** Gross Findin s·-:: 

# iReport 48-136 Appendix 2



### ZM/S-D Strain: Sex:

__...:M.=:a:.::l:.,:e;-._____ Age: 

### 6-7 weeks Date of Death: --'7'-1./-=2""5
.... /.:::.8;:.5

_ Micro Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-17037 PAT H 0 LOG Y 

## -----_._--

E EM Kodachrome: Gross Fate: -l.-D Path. Pace II 32 Test No. : 14 Sample No.:**_~4:..:8_-..:..1.::.2::...1.;;;.B** _ Sample Name: Aldicarb 

### DeM Treatment Level: 0.0087 mg/liter

Clinical Ristor·

Gross Findin s Eyes

- Lenticular opacity, red stained. Perinasal area - Red stained. Lungs

- Dark pink, all lobes. Trachea, Liver, Kidnevs, and Bronchi

- No gross lesions.Gonads I Trachea, Liver, Kidneys, and Bronchi

- No gross lesions. R E COR D

------

Clinical Historv Test Name: Acute Aerosol Inhalation Toxicity (LeSO) Species: Rat Animal No.: 

### 85-17038 PAT H 0 LOG Y



## ---------

0.0087 mg/liter _E _EM Kodachrome: Aldicarb PCM 14 

### t.8-121 B

XD

Perinasal area - Red encrustation. Lungs

- Dark pink all lobes. - Red encrustation, bilateral.

- Right

- Lenticular opacity. Path. Pace 1133 Sample No. : Sample Name: Treatment Level: Fate: Test No.:

Bod\' Weight '**'~** Report 48-136 

# ·W Appendix 2 

# Lf**.~** 

## -, ! 

## -f Strain: ZM/S-D "**~. _--,,~.ao..;..~.**  -:. Sex:

--.... :"":"":'"":'-o-f-p-e-a-t-h-:

- Age:

# ~~ 5

# ~:~h v

# =.'.~ Gross Micro 

# l 

# i

:

# ,.

i ·i**1======================~~'=h.================If:.r** Gross Findinl'.s**t-----------------"-';...;;.c~~;;.;..;.c==:..-------r_-----.,..----_.;.j~.**

# 7~~::5**~:~\;~:**

# I

Liver ,]:'. I-=.:..;.:::.:...

__**.-+-----~** 

### .:

Kidnevs Spleen !!!

.

. .

,: 

## I;

......

### ,:

.' c,,'



### .D·
..**~~o.h;,meC>u~Death~:.....··**
"**.>.;:.~'" ~,**  :.,Age:

__**~6=-.L7--:&1.w.l:.e.l:.e.ll.k::..s** __ Test Name: Acute Aerosol Inhalation Toxicity (LesO) Species: Rat Animal No.: 85-17039 Report 48-136 Appendix 2

Strain:**_~Z~M!J./...ilS~-M.D** _ Treatment Level: 0.0087 mg/liter Sex:

__:.:Ma"'""'-l
... e

_ Fate: l.D E EM Date of Death: -L7L/2
.......**'i~/6~).J...·** _ Kodachrome: Gross Micro Path. Pace (134 Test No.:

__**....:1~4~_** Sample No.:**_.....:4~8~-....:l:.o:2:..:.1~B** _ Sample Name:**_~A=l~d=i~c~ar=-b~D~C::::M:.:...** _

Clinical History

Gross 'Findings Date Time Cervical lymph nodes 

### - Enlarged 

### 2X normal. 7/25/85 

### 2:30 pv 

# .. OrQan \oil" (0) Lungs 

## - Dark pink all lobes. Liver Kidneys



## - Red stained. Spleen



# - Left 

## - Lenticular opacity. Heart Brain Kidneys

- Mild hydronephrosis. Pituitary Adrenals };asal cavity, Trachea, Liver, and Bronchi 

# - No gross Gonads

lesions. Body Weight

-

I

.. Prosector:
.**-H~lS** t**~':** I :nd 

### !, i

. -, '. , . '. .. D = Spontaneous Death _'
, '**~.** E- Euthan:i.zed'II

Report 48-136 Appendix 2
Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No. : 

### 85-17040 Path. PaCe I' 35 Test No.: Sample No.: Sample Name: 14 48-121 B

Aldicarb DCM Strain: ZM(S-D

Clinical History



## -

..: :::

:, Gross Findings Date Time -.'

..**~es** 

### - Red stained, bilateral. 

### 7/25/8; 2:36 P}j



# - Left


## - Lenticular opacity. On,:an t<..**(~)** Liver**Lun~** - Dark pink, all lobes. .Kidnevs Spleen**~asal** cavitv, Trachea,**Live~.** Kidneys. and Bronchi 

### - No Heart gross lesion",. Brain Pituitary Adrenals Gonads ; Body \-"eight 

### ,

.",

,**,.~** Prosec':tor: .-·}l_\:St

## eelX: -'. " 

### " .- 

### D
...Report 48-136 Appendix 2 p",th. Paee [I 36
85-17447 Age:

__....;6::..-....;7'--'w::.;E::,;e:,;k'"'s"---_ Animal No.:**--='"'-~:....;..:...-** Rat Strain:**_....!Z~M:J../...:::S::.-!::.D** _ Acute Aerosol Inhalation Toxicity (LC50)

Sex:**_--:.Ma=-<l:.l:e~** _ Date of Death:**....!8~/:...J1:.J./..s:8~5** _ Micro Species:

Gross Test Name: Test No.: 14 Sample No.: 48-121 B Sample Name: Aldicarb DCM Treatment Level: 0.0060 mg/liter 

### E EM Kodachrome: Fate:**~D**

. !,

.... Clinical History

= Gross**Find1n~s** Date Time Eyes
- Red encrustation, bilateral. s7f785 9:26 A:-l 

### 0,.",,1'\ 1,.,'t (Q) Perinasal area - Tan stained. Liver Kidnevs Lungs

- Dark red, all lobes. Snleen Heart liver

- Mottled light tan and brown. Brain' Pituitar" Trachea, Kidneys, and Bronchi 

# - No gross lesions. Adrenals Gonads Bod"**\o;'ei~ht**

I

ProsectOT·:'**H~:St~il** ",.

'I t:lC 

## ! .' 

### ., D Spontaneous
..Death 

### E" Euthanized :E!':.

### lut:ha~~(zed:wh~·ri:,:nCT'ibu~= 

### ·,.····.'.··.,6\1'"f'r·)·· •.•··••·

# •

Report 48-136 Appendix 2

<

"
6-7 weeks -_....::.....:........:::.::.::.::.:.=- .•. Age: 8/1/85

____......;:;.:-=..:c...::.:: . , 

### ZM/s-D Strain: R E COR D


### ------

Sex:

__M:..:.a:::.l::.e=-

_ Date of Death: Micro Gross Test Name: Acute Aerosol Inhalation Toxicity (Le50) Species: Rat Animal No.: 85-17451

Kodachrome: Path. Pa8e f.1 37 Test No.: 14 Sample No.:**--:4:::.:8~-...:1:.:2:..:1~B"--** _ Sample Name: Aldicarb DeM Treatment Level: 

### 0.0060 mg/liter Fate: -lLD E EM
=-:.' Clinical Historv ;' :.' ,.;!

- ..

..

-

.. 

### = '. Gross Findings i: 

## . :; Date Time 

### .. . ' Eyes 

# - Red encrustation. lenticular opacity, bilateral. 8/l/R" g'3,} A.'1**~L~** •.... OroaT'l Wt (\;!)**\'~** Lungs 

# - Mottled light and dark red. all lobes. Liver t·· Liver Mottled light and brown. Kidnevs



# - tan Spleen ..
--- f) Heart**:~** l'asal cavity, Trachea, Kidneys, and Bronchi 

## - No gross Brain**f~~** .lesio"ns. 

# ---- ? :' Pituitarv .,**!.l~** Adrenals (' Gonads Body Weight I**~: f.~** 



### ..

"

..

i

Prosector:**H~lSteel** 

# -'" ..',u_
D = Spontaneous Death E
.. Euthanized

. E}! Eu

### thaniied;;henlilor{bu~dJ: ,::"**r·;··i:>·i;i77J·~;**8-9 weeks 8(14/85 Species: Rat Animal No. : Strain: ZM(S-D Sex: Male Age: Date of Death: Gross Micro Test Name:**~A~c:..!:u!..!:tc!:e~A~e.::.r.!:::.o2.s~o=.l_l=.n!.!;h~a~l~a~t:..:i~o~n~T~o::.:x~i~c.=i.::.t.z.V-.l:.( L~C=:.50~)!..-**  __ 85-17442 Report 48-136 Appendix 2 path. Pace II 38 Test No.: 14 Sample No.: 48-121 B Sample Name: Aldicarb in DCM Treatment Level: 0.0060 mg/liter Fate:

__ D -LE

__ 

### :EM Kodachrome: Clinical Historv

Gross Findings Date Time Nasal cavity, Trachea, Liver, Kidneys, Bronchi, and 8) 14T85 8:39 P"**lesi~** oJ Lungs 

## - tio gross Orp'::tn Wt" (p' "\ Liver Kidnevs 501een Heart Brain Pitu:harv Adrenals I Gnnads I Bodv Wei£ht

I I ProSector: H:-!Stecl. 

# I rod
.. 

## i

I' D Spontaneous Death . E ., Euthanized

.**E~l** = Euthaniied.whenrncribu:lc 6/77**~,** . >/.;Report 48-136 

### t Appendix 2 Path. 

### Pace f.l 39**!!!.!!Q~QQr** 

### !!.f.Q!1! Test No. : Test Name: .

## . 14 Acute Aerosol Inhalation Toxicity (LC50) Sample No. : 48-121 .B Species: Rat Animal No. : 85-17L.t.8 

### 1



# - Sample Name: Aldicarb in DCM Strain: ZM/S-D**:.~** Treatment Level: 0.0060 mg/liter Sex: Male Age: 8-9 weeks 

# ) Fate: D 

### -!..E _EM Date of Death: 8/14/85**:.~.** Kodachrome: Gross Micro 

# 1 Clinical History

.;
·f , 

# 11 Gross Findings 

# n Da'l:e Ti!!\e i 

### ." 8114/85 8: 33 .A}! I .r 

### • Nasa.l cavity. Trachea, Liver. Kidneys. Bronchi. and OrI!.an Wt (I!.) No**lesi~** Lungs

- gross Liver

. 

### , l Kidnevs ! Spleen , Heart 

### '

'" 

### Brain Pituitarv ;: Adrenals " 

### " , Gonads Body "'eight

..**~-** :

I

. ,

D Spontaneous Death E Euthanized8-9 weeks 8/14/85 Age: ZM/S-D Strain:
Date of Dea':h: !-licro Male Sex: Gross Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-17449

Kodachrome: 0.0060 mg/liter**~E** EM 14 Rt:port 48-136 Appt:ndiz 2**S&~?le ~o.:**  __**~4~8~-~1~2~1_B~** ___ AId 1 °carb l'n DCM s.::;>le Name: :reatment Level:**:~st ~o.:** 

E '" .Euthanized Spontaneous Death D 

# - C1 inical History -
,



### ...... I I :
, Gross Findin25 ; Date Time
0**~3sal** cavit)'. Trachea, Liver, Kidneys. Bronchi, and 8/14/85 8:25 A.': I 1 Lungs

- No gross lesions. 

### 0,..,,,,,., Wr (" ) I I I Liver 

# I Kidneys Spleen I Heart Brain Pituitary Adrenals Gonads 

# I Body Weight I 

### i 

# I i

Prosector:,**'H~:S(c",:** ,<

, ;.-, ::--,:: 

## !Report 48-136 J.., Appendix 2

Age: ZM/S-D Inhalation Tox;City (LC50) Animal No.: 85-J7407 ,R E COR D

------ Acute Aerosol

Sex:

__"l;MjQau.".l::e

_ Species: _-'-,--..QR

... a....

t

_ Strain: Aldicarb 

### DeM Sample No.:

__**~4~8~1~2~J~B~** ___ Sample Name:
Fate: ....L-D Treatment Level: 0.0020 mg/l;rer Path. paee Ii 41 Test No.: 14 

## 1

__.J,;6L,:-:.L 7......w

Wl:.el:. ek

U5-'

# q E EM Date of Death: -'-ZJ,./...1 3J,.J,L/.c. 8.J.5 

### t. Kodachrome: Gross Micro :**F=============::~=;==:=§==================_.:'I** 1-- , ....::.C=.l=.in::.J.=.·**c:::a:::l:....:H~i:.:s::.:t::.:o:.::r:..l-v** _l ;) 

### ,:i

Gross**Findin~~** 

### __,-----__,------l Eyes

- Red encrustation, bilateral.**t===;i;!,Da~t**g,e**==F==Tb;i~ml1Jie"===I'** ,! 7f<1/P,r:.. 10·;') AM Perinasal arga - Red stained.' Liver Right kidne:

- Mild hydronephrosis. Kidnevs Soleen**T~achea,** Liver, Left kidney, Bronchi, and Lungs

- No gross lesions. Heart Brain I--=-P.:.i.::,tu::.J.:::.·t::;a:::;rv::..:.._I- -1% :

Adrenals Gonads Bodv Wei>tht

D : Spontaneous Death E'"

### E~thani~~d'Report 4B-1 3h Appentli;.; 1 Pa:h. Pa[je II 41 Test No. : 14 Sample No.: 48-121 B Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-17406 sample Name: Aldicarb in DCM Treatment Level: 0.0010 mg/liter Sex: Strain:

__**~Z~M'-L/~S:...-.::;D** _ Male Age:**_...:B~-_9~w:.:e:..le:..!k~s~**__ Fate:

__ D JLE _EM Kodachrome: Gross Date of Death: Micro 8/13/B5

".'.';.

, -.;<':'/:,-: ,;**,.,.~:'** ',-.",-'.': Clinical Ristorv

Gross l'indin2s Cervical lvmph nodes Enlarged 2X normal.**Da~"** Time



### - 8/13 185 8:32**~l** Multiple red foci disseminated Oro,," \-iT 

### (;) Lungs 

# - over all lobes. Liver Right kidney Mild hydronephrosis. Kidnevs



# - Soleen Nasal Trachea, Liver, Left kidney, and Bronchi Heart cavity, 

# - Br.;-i.n**~o** gross lesions. Pituitary Adrenals Gonads Bodv Weieht



# I I I I Preis.ector: H:-!S

### t:e,,: ., "-'';::;': , I': ",' ' •.•t....: D";' Spontaneous DeathPath.**Pil~e** /1 4J PAT H 0 LOG Y R E COR D



## --------- ------ Report 48-136**Appendi~:** 2 Test Name: Acute Aerosol Inhalation**Toxi~ity** (LC50) Test No.:

__--=1""'4'--_ Sample No.:

__**~4~8~-~1~2~I~B** __ Sample Name: Aldicarb in DCM Species: Rat Strain: Animal No.: 

### 84-17408 ZM/S-D Treatment Level: Fate: 

### D 0.0020 mg/liter -X-E _EM Kodachrome: Sex: Gross Male Date of Death: !-licro Age: 

### 8-9 weeks 8113f85 Clinical Historv 

### i

Gross Findings Right kidney

- Marked hydronephrosis. Nasal cavity, Trachea, Liver, Left kidney, Lungs, and Bronchi

-**~o** gross lesions. Date 8/11/R'i Liver 

### Kidne'\'s Time

Spleen Heart 

### Brain Pituitary Adrenals Gonads , Body t,Teight '

Pro'sector: ", _.!=.•.

### '<

## D'}.;_~~i6ntarie!?,us Death

# •

Report 48-136**Appendi:~** 2 Path. P;i[\€ /144 Test No.: Sat:lple No.: 14 48-121 B Test Name: Acute Aerosol Inhalation Toxicity (LeSO) Species: Rat Animal No.: 85-17409 ZM/S-D Sample Name: Aldicarb in 

### DeM Treatment Level: 0.0020 rna/liter Strain: Sex: _-.....JMai:!SL1""elL-

_ Age:**_----'8~-::.9~w~e=.;e=.;k~sL_** Fate:

__ D -X....E _EM Kodachrome: Gross Date of Death:**~8CL/~1_""3L/~8~5** _ Hicro

.,:';_
- ,j", ••"••••• "/ ". ',c.
. -: :',.**:;,.:-,'~_** (;:;;"**..:.~./'.~'::";** •**/~:**



# ..**"'i;§~(tb";~~ih~'~:i,i~~S~,~~'f(h~t'~~lt~t\ti;:iil~** . "",' :,",

- .',""::'-**!·E",Euth~ti:ized .~."**  I Clinical History 

## f

I


# - Gross Findings Date Time Right kidney

-**~a:!.d** hydronephrosis. RII1/A:; B: "2 101 Oroan \\,T" 

## .,,,) :\a5al cavitv. Trachea. Liver, Left kidney. Bronchi. and Liver Lungs

- :\0 gross lesions. Kidnevs I Suleen Heart I Brain Pituitary 

### I Adrenals Gonads 

# I Bod"\' W'ei12ht 

## -


# I I I I i I (" I I

, 

### ! 0 ! I

# I , ., -, .**~~'\'** .......'

### ..

## ":

.. Report 48-136 Appendix 2

Strain:
Date of Death: Micro (LC50)
.

# -i --7r· 85-17410 

### "*:/**---;~** ZM/s-D '\


# ---.......**L.><.-=---------~t~·** Sex:

__**L<MSila..&.l~e______** Age: 8-9 weeks 

# -X Test Name: Acute**Aeros~1** Inhalation Toxicity Species: Rat Animal No.:

....A.-E EM Kodachrome: Gross 14 

### 48-121 B

D Sample Name: Aldicarb in DCM Treatment Level: 0.0020 mi/Jjter Path. Pae.e (/45 Test No.: Sample No.:

Fate: 8/13/85 ;'

______.............WoI..:.....-

.,**F='=============:~=:==~~=======;;;;;;;;;;=========='** Clinical History

Gross Findings Right kidney

- Marked hydronephrosis. Date R/l1/R" Time R·b" AM Wt (I") Nasal cavity, Trachea. Liver. Left kidney. Lungs, and Bronchi

-**~o** gross lesions. Liver Kidnevs Spleen Heart Brain**~P.:::i.::;t.::;u=i=t~a.:;.n':"'_+-** --I" Adrenals**~==-C::'::-:='---+** --1'"

### FG~o~n~ao;.d~s===+i=======1 ' Bodv Weight

.



## .... '1 '.

D**Spontaneou~** DeathPath. Par,e II 46 R E COR D


### ------ Report 48-136 Appendix 2
Test Name: Acute Aerosol Inhalation Toxicity (Le50)

8/12/85 Age: 9-10**~eeks** Animal No.: 85-17240 ZM/S-D
Date of Death: !Hcro Sex:

__-.::!M!2 a.:

1.s. e

_ Species:

__**R=a~t** _ Strain: 14 48-121 B Sample Name: Aldicarb in DCM Treatment Level: 0.00082 mg/liter Fate:

__ D ...L-E

__ EM Kodachrome: Gross Test No.: sample No. :

I Clinical Ristorv f---'

Gross Findings n"te Time 8/1i/85 8: 11 

### A.'": Nasal**cavity~** Trachea, Liver, Kidneys, Bronchi, and 0,..."a'"**l,;'~** (p) Lungs 

# - !\o gross le:;ions. Liver 

### I Kidne"s I Snleen I Heart I Brain Pituitarv Adrenals. Gonads Bod\' ,,"'eh:ht

I

PrOSeCtOr: 

### •**H~:st.:S'·** 

# I**:~.,** . 

### .i ''"-' '-:,,::;q' ,::. D Spontaneous'Death E ::**Euthariiz~d_.** "\-

....... ' ',,-·f ',',:Path. Paee I J47 R E CDR D


### ------ Report 48-136 Appendix 2

Treatment Level: 0.00082 mg/liter Test No.: Sample No.: SampIe NalI'.e: Test Name: Acute Aerosol Inhalation Toxicitv (LC50) Species: Rat Animal No.: 85-17242 48-121 

### B

8/12/85 Age:

__**9:....---'1:....O'---'w;..,;e:.;e""k:.::s~** ZM/S-D Strain: Sex: _--=Ma=l::.;e:....-

_ Date of Death: Micro --!..E EM Kodachrome: Gross Aldicarb in DCM 14

D Fate:

..ct 

### l-j 

# , EN = Euthanized ",hen moribund E - Euthanized

Clinical 

### Historv 

### I

Gross**Find1n~s** 

## I Date Time 8/1218S 7:57 AN !tight kidney

- l".arked hydronephrosis. Or"an Wt (p) Trachea. kidney, Bronchi, and Liver Nasal cavitv, Liver, Left Kidne"'s I Lungs

- Ko lesions. gross Spleen Heart Brain Pituitary Adrenals Gonads 

# I Bod\' h'eighti

Prose-ctcr:**H~!St",,:'**
..d 

### D - Spontaneous DeathReport 48-136 Appendix 2 P3th. Paf;e I! 48 rest No. : sample No.: 14 48-121 B Test Name: Acute Aerosol Inhalation Toxicity (Le50) Species: Rat Animal No.:**85-1724~** Sample Name: Aldicarb in DCM Treatment Level: 0.00082 mg/liter Sex: Fate:

__ D 

### -LE

__ EM Kodachrome: Gross Strain: ZM/S-D Male Age: 9-10 weeks Date of Death: 8/12/85 

### Micro**~rosector: HX~teel**  md Cl' . 1 H I l.nl.ca istorv

Gross Findings 

## I n"te Time**il/l,/.q~** .q: 05**A}~** Kasal cavity, Trachea, Liver, Kidneys, Bronchi. and Oro",,, \<'t (,. ) Lungs

- No gross lesions. Liver Kidneys Spleen Heart Brain Pituitarv Adrenals Gonads I Bodv Weieht

-,



# -

- ----- ---._,


### D = Spontaneous Death E • Euthanized 

### EM = Euthanized when**rnoribu~d** 6/ii

### R E COR D



### ------

Clinical Histor Age: 

### ZM S-D Test Name: Acute Aerosol Inhalation Toxicit Animal No.: Sample No. : I.A-I21 B Species: Rat Sample Name: Aldicarb in DCM Strain: Treatment Level: 0.000B2 mg/liter Sex: Male Fate: D 

### -LE EM Date of Death: Kodachrome: Gross Nicro Path. Pace 1/49 Test No.:

__**~1....;4**__

Gross Findin 5 Right eve - Lenticular opacity. Nasal cavity, Trachea, Liver, Kidnevs, Bronchi, and Lungs

- No gross lesions.



### D

## = Spontaneous Death E • Euthanized E" Euthanized when rncribund .6/77.-Path. Par,e (I 50 Report 48-136 Appendi :.: 1 Test Name: Acute Aerosol Inhalation Toxicity (LC50) Species: Rat Animal No.: 85-17251 lest No.: 14 Sample**~o.:** _....;4::tJ8
.... ---'l

... 2
.....

1'--"5:.-

_ Age: ----<9_-
.... 1**Q~w....,e**.... e .... k
... s'-

__ S/l2lBS ZM($-D Strain: Sex:

__...JMaI:lQ.1 01.J

e
_ Date of Death:

____-Ju..<...J
............... _ 1'1icro Gross sample Name: Aldicarb in DeM Treatment Level: 0.00082 mi(Jiter Fate: 

### D -X-E

__ EM Kodachrome: Cl' i 1 H' I**~n** ca lstory

Gross Findings 

## I Date Til:le_. R/l?/ll,<; R'li**A\~** Cervical lymph nodes 

# - Enlarged 3.5X normal. Or12an**~t** (,,) liver Right kidney
- Mild hydronephrosis. Kidnevs I Spleen Kasal cavity, Trachea,**~,** Left kidney, Bronchi. and Heart Lungs

- No gross lesions. Brain Pituitarv Adrenals Gonads

- Bodv**~eight**

I



## - I I I Prosector:**~~Ste~}** ; 

## we D
.. Spontaneous Death E so Eutha11ized Euthanized**~hen L.~"i~u~:**  

# 6:';-:

# • BUSHY RUN RESEARCH R. D. 4,

...lIon Road, Export, Pennaylvanla 15832 

# CENTER

UCC BUSINESS CONfIDENTIAL: Not to be released outside UCC without the written consent of Dr. 

### R. L. Baron, Manager of Toxicology, Union Carbide Agricultural Products Company. Project Report 49-65 Revised 

### 7 Pages May 15, 1986



### Aldicarb in DeM (3 

### Solutions) Acute Peroral Toxicity Study

- 

### D.O.T. Classification Sponsor: Union Carbide Agricultural Products Company



## * 

## * * * * SUMMARY A rat peroral toxicity test was completed on each of 3 concentrations of aldicarb in DCM. The procedure followed the standard BRRC protocol modified to conform to Department of Transportation (D.O.T.) test conditions. Results from this study, expressed in terms of each solution as received, are as follows: Peroral, Rat (Fasted)**1.0~** 

### Concentration Males: SO mg 

### of solution per kg of body weight (b.w.) killed 5 of 5. Females: 50 mg 

### per kg 

### b.w. killed 5 

### of 5. 

### Combined: 50 mg 

### per kg b.w. killed 10 of 10. 

# -**Q.5~** 

### Concentration Males: 50 mg 

### per kg b.w. killed one of 5. Females: SO Mg 

### per kg b.w. killed 3 of 5. Combined:**~per** kg 

### b.w. killed 4 of 10.**Q.3~** 

### Concentration Males: SO mg 

### per kg b.w. killed 0 of 5. Females: .50 

### mg per kg b.w. killed 0 of 5. Combined:**~er** kg 

### b.w. killed 0 of 10.

Bushy Run Research cenler A Jolnl Mellon Instltule-Unlon carbide Corporation Operation

### Report 49-65 Revised Page 2 MATERIALS Three samples of aldicarb in DCM (dichloromethane) were received from Union Carbide Agricultural Products Company, Research Triangle Park, NC on February 17, 1986. Each sample, as received by the Bushy Run Research Center, consisted of 100 

### ml of a colorless, transparent, non-viscous liqUid. The containers bore the identification numbers HTS6806AC (1.0% Active ingredient), HTS6806AD**(O.5~** 

### active ingredient) and HTS6806AE (O.3X 

### active ingredient). BRRe Sample Numbers 49-45, 49-43 and 49-44 were**~ssigned** 

### to the respective materials. The BRRe Project Humber for work on these samples is 86-03-10722. The samples were dilutad in corn oil (Mazola). The dilutions were mixed on a magnetic stirrer for approximately 

### 5 to 20 minutes before dosing and during dosing.**~tmental** 

### Animals 

### Male and female Sprague-Dawley albino rats, weighing bp.tween 200 

### and 250 g, 

### were used because of this laboratory's past experience with this species and strain. The rats vere obtained from Harlan Sprague-Dawley, Inc. (Indianapolis, IN) and were acclimatized for at least 5 

### days before they were dosed. Upon receipt, they were housed in Room 109, 

### where they were subsequently dosed and observed until sacrifice. All rats were assigned unique animal numbers and were identified by toe clipping. The rats were housed in cages, 5 

### per cage. with wire floors under which deotized animal cage board was placed. They were maintained on Ralston-Purina certified rodent chow and water provided by 

### an**auto~atic** 

### water system. Water was supplied by the Municipal Authority of Westmoreland County (Greensburg, PA) 

### and was available at all times except during the actual dosing period.
TEST PROCEDURE An 

### appropriate amount of suspension was administered by stomach intubation through a commercial 16 gauge (3 in.) ball-end stainless steel needle attached to a disposable syringe. The exact amount of sample dosed for each rat was recorded on the dosing sheet. Five male and 5 female rats were dosed at 50 mg/kg b.w•• the Department of Transportation (D.O.T.) Class B poison level. The rats were fasted overnight**(approximatel~** 

### 18 hours) before dosing. Dosed rats were observed frequently for signs of toxic effect on the first day of the test and twice a day (except on weekends or holidays) thereafter. Weights were recorded on the day of dosing and at 7 and 14 days after dosing. After 14 days, all survivors were sacrificed and necropsied. The above procedure was performed for each of the 3 concentrations of aldicarb in DCM.

### Report 49-65 

### Revised Page 3 

### RESULTS Results from the peroral study are given in Tables 1, 2 and 3 for the**1.O~, 0.5~**  

### and**0.3~** 

### concentrations, respectively. AIlS male and 5 

### female rats died following peroral doses of 50 mg/kg b.w. of the**1.O~** 

### aldicarb in 

### DeM. Signs of toxicity included sluggishness, tremors, salivation, lacrimation and red tearing. Deaths occurred at 15 minutes to 1.5 hours. At necropsy,**lur~s** 

### were dark red and periocular fur had a red crust. The 0.5% concentration killed one of 5 male rats and 3 of 5 females. Tremors, salivation, red tearing, kyphosis, piloerection, diarrhea and red to brown discharge around the nose and eyes**~ere** 

### observed. Time to death ranged from one hour to one day. Survivors recovered at 4 to 5 days. Gross pathologic lesions included pink lungs, red periocular fur and one cecum with watery fecal material. These conditions were apparent in the victims. Except for one female with enlarged, fluid-filled uterine horns, there were no remarkable gross findings among the survivors. Intubation of the**0.3~** 

### aldicarb in 

### DCM produce death in any of 5 male or 5 female and perinasal fur and diarrhea were noted. There was no remarkable gross pathology in enlarged, fluid-filled uterine horns. solution at 50 mg/kg did not rats. Red crust on the periocular

All animals recovered by one day. the males. One female had

REASON 

### FOR REPORT REVISION The original report on a1dicarb in DCM, dated April 23, 1986, has been revised. The only changes that have been made are editorial in nature. There have been no changes in the results originally report.

### Report 49-65 Revised Page 4 

### Reviewed and Approved by: 

### S-IS-

# 'i Date 

# II/2**4tl~-:!v/v.-** 

# -"';-:15--

# J6 Ronald 

### S. Sleslnski, Ph.D. Date Assistant Director

Acknowledgements: Single Peroral Test

Manager. Pathology Department Fred R. Frank, Ph.D. Director

Susan M. Christopher, B.S. Research Technologist Dana L. Cline, B.S. Laboratory Technician Edward 

### H. FOWler. Ph.D., DVM Associate Director Date

Animal Care Archives

WPC/esk/0628P-l 05-09-86 Michael J. 

### Cardella, AALAS Cert. III 

### Supervisor I 

### Edgar 

### C. Rimler, B.S., AALAS Cert. 

### I Technologistrable ), 

### Peroral Intubation. Single Dese to Rats Material: Aldicarb in DCM

- 1.0% Concentration Sample No.: 49-45 Dosage,
.Deadl mg/kg Dosed Days to Death Weight**Chang~** 

### 7 Days 14 Days**Si&n~_~Toxi~ltY** 

### Male Rats Gross PathololtY

50

50 5/5 0,0,0,0,0

SIS 0,0,0,0,0 

### Tremors, salivation, lacrimation, red tearing in 5 at 12 min. Death at 15 min to 1.5 hr.
Female Rats Sluggishness, tremors, salivation in 5 at 10 min; kyphosis, piloerection in 1 at 20 min; red tearing in 5 at death. Death at 16 to 33 min. Lungs dark red; red encrustation on periocular fur.

Lungs dark red; red encrustation on periocular fur.

LDSO's: Males: 

### < Females: Combined: 50 mg/kg; sample dosed as a 50% (w/v) 

### dilution in corn oil. < 

### 50 mg/kg; sample dosed as a 50% (w/v) dilution in corn oil.



### < 50 mg/kg; sample dosed at a 50% (w/v) dilution in corn oil.WPC/esk/0628P-l05-09-86

"d::<l III III QQ"t:l III 0

'"1 1./1,.,

l:-- \0 I 0- 1./1 ::<l III < 

## .... lIJ III PoTable 2 

### Peroral Intubation. Sinfi'e Dose to Rats Material: Aldicarb in DCM

- 0.5% Concentration Sample No.: 49-43 Dosage, 

## !!!.&LK& Deadl Dosed Days to Death**Wei~hLChan~e.** 

### 7 Days 14 Days Sbns_oLToxicity Male_Rats Gross PathololtY

50

50 1/5

3/5 1

0,0,0 12 

### to 45 (mean=32)



### 33 and 

### 59 (mean=36) 64 to 79 

### (mean=72)



### 32 and 

### 51 (mean=42) Tremors, salivation in 5 

### at 30 

### min; kyphosis, piloerection, diarrhea, red crust on periocular and perinasal fur at 1.75 

### hr. Survivors recovered at 5 

### days. Female Rats Tremors. salivation in 5 at 20 min; red tearing in 1 

### at death. Death of 3 

### at 1 

### to 1.5 

### hr. Brown crust on periocular fur of 2 

### at 4.5 

### hr. Survivors recovered at 4 

### days. In victim, lungs dark pink; cecum filled with watery fec&l material; eyes with red encrustation. In survivors, nothing remarkable.

In victims. lungs dark pink; periocular fur stained red. In survivors, uterine horns of 1 

### enlarged. filled with clear fluid.

LD50's: 

### Males: ) Females: Combined: 50 mg/kg; 

### sample dosed as a 50% (w/v) dilution in corn oil. ( 50 

### mg/kg; sample dosed as a 50% (w/v) dilution in corn oil. ) 50 

### mg/kg (4/10 

### dead); sample dosed as a SOX (w/v) 

### dilution in corn oil. WPC/esk/0628P-l 05-09-86**~:>:l** III In OQ'tl /I) a '1 O'rt .po <0 IaUt :>:l (1) 

### .<,l

## Vji~TabJ..Ll 

### Peroral Intubation. Single Dose to Rats Materic.l: Aldicarbin_DCM

- 0.3;1; Concentration Sample No.: 49-44 11 

### Dosage, 

### !!!.&L1sL Deadl Dosed Days to Death WeiAht**ChanA~.-R** 

### 7 Days 14 Days SiRns of Toxicity Male Rats Gross PatholoJtY

50 0/5 

### 38 to 57 (mean=46) 51 to 87 (mean=70) Red cruat on periocular and perinaeal Nothing remarkable. fur, diarrhea in 5 

### at 2.5**h~.** 

### Recovery at 1 day. Female Rats 50 0/5 

### 19 and 23 (mean=22) 25 and 31 (mean=28) Red crust on periocular and perinasa1 fur of 5 at 3.25 hr. Recovery at 1 day. Uterine horns of I 

### enlarged, filled with clear liquid.

LD50's: Males: ) Females: Combined: 50 mg/kgj sample dosed as a 

## sox (w/v) dilution in corn oil. ) 50 mg/kgj sample dosed as a SOX (w/v) dilution in corn oil.

) 50 mg/kg; sample dosed as a SOX (w/v) dilution in corn oil. WPC/esk/0628P-l 05-09-86

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### CERTIFICATE OF AUTHENTICITY
THIS ISTOCERTIFYthatthe microimagesappearingonthis microficheareaccurate and complete reprodul::tions of the records of U.S'. Environmental Protection Agency**doc:ume~ts** u delivered In thll regular course ofbusinesB for**mlcro~.** Data prodUced'f}

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# 93 'fq.f1M.J.Ak**~()** (Month) (Cay) (Year) Camera C?perator

Place Syracuse (City) NewYork (Slale) 

# a AMTEK corp