# Patent

## Title
Methyl and ethyl substituted pyrrolidine compounds and methods for selective inhibition of dipeptidyl peptidase-IV

## Abstract

The present invention is directed to pyrrolidinylaminoacetyl pyrrolidine boronic acid compounds that display selective, potent dipeptidyl peptidase IV inhibitory activity. These compounds are useful for the treatment of disorders that can be regulated or normalized via inhibition of DPP-IV including those characterized by impaired glycemic control such as Diabetes Mellitus and related conditions. The compounds can be administered alone or with another medicament that displays pharmacological activity for treatment of these and other diseases.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. application Ser. No. 10/514,575, filed on Oct. 27, 2005, which is a national stage application of PCT/US04/037820 filed Nov. 12, 2004, which claims priority to U.S. provisional application No. 60/519,566, filed on Nov. 12, 2003, U.S. provisional application No. 60/557,011, filed on Mar. 25, 2004, and U.S. provisional application No. 60/592,972, filed on Jul. 30, 2004. This application is also a continuation-in-part of U.S. Application No. 60/676,808, filed on May 2, 2005. These applications are incorporated herein by reference.


FIELD OF THE INVENTION
The present invention relates to methyl or ethyl pyrrolidinylaminoacetyl pyrrolidine boronic acid compounds and their use as selective inhibitors of post-proline/alanine cleaving amino-dipeptidases, particularly dipeptidyl peptidase-IV (DPP-IV). The invention also relates to methodology for employing such pyrrolidine compounds, alone or with another medicament, to treat a DPP-IV-related disease, including but not limited to disorders characterized by impaired glycemic control, especially Diabetes Mellitus and related conditions. Thus, the invention has applications in the medicinal, chemical, pharmacological, and medical fields.
BACKGROUND OF THE INVENTION
Dipeptidyl peptidase-IV (DPP-IV) is a serine protease that belongs to a group of post-proline/alanine cleaving amino-dipeptidases. DPP-IV catalyzes the release of an N-terminal dipeptide of any configuration from proteins, and preferably, the dipeptide contains an N-terminal penultimate proline or alanine.
The physiological role of DPP-IV has not been established fully. It is believed to play an important role in regulatory peptide metabolism, which, among other things, controls various physiological functions including but not limited to glycemic control and insulin sensitivity. In particular, DPP-IV has been implicated in the control of glucose metabolism because its substrates include the insulinotropic hormones, glucagon like peptide-1 (GLP-1) and gastric inhibitory peptide (GIP), which are inactivated by removal of their two N-terminal amino acids.
In vivo administration of synthetic inhibitors of DPP-IV prevents N-terminal degradation of insulinotropic hormones including, GLP-1 and GIP, resulting in higher plasma concentrations of these hormones, increased insulin secretion and, consequent improved glucose tolerance. Therefore, such inhibitors have been proposed for the treatment of patients with impaired glycemic control such as Diabetes Mellitus and related conditions.
This proposal has significant difficulties, however. Additional dipeptide cleaving amino-dipeptidases have also been discovered, including DPP-VII, DPP-VIII, DPP-IX, and fibroblast activation protein (FAP), which can have substrate and inhibitor specificity similar to DPP-IV. The precise physiological role of each of these dipeptide cleaving enzymes is not well defined. But, their propensity to cleave N-terminus dipeptides from proteins in general indicates that these amino-dipeptidases are involved in many physiological cycles. Thus, the difficulty concerning inhibitors of DPP-IV is that they can also affect the other members of the enzyme group. The evidence indicates that, for example, other inhibitors of DPP-IV, which also inhibit the other amino-dipeptidases such as DPP-VIII, will cause toxic effects in animals.
Accordingly, a need exists for compounds that are useful for inhibiting DPP-IV without an adverse event profile that precludes chronic administration.
SUMMARY OF THE INVENTION
The present invention is directed to selective DPP-IV inhibitors and methods of use that are effective in treating conditions that may be regulated or normalized by inhibition of DPP-IV. More particularly, the invention is directed to methyl and ethyl pyrrolidinylaminoacetyl pyrrolidine boronic acid compounds. These methyl and ethyl pyrrolidinylaminoacetyl pyrrolidine boronic acid compounds are useful at effective doses for treatment of malconditions associated with DPP-IV activity and is a selective inhibitor of DPP-IV.
The methyl or ethyl pyrrolidinylaminoacetyl boronic acid compounds of the invention (hereinafter the pyrrolidine compounds of the invention) have a structure represented in part by Formula I.




The substituents and bond designations of formula I include R1, which is methyl or ethyl; R2 and R3, which, independently or together, are —OH, —O−M+, wherein M+ is a cation, a hydroxyl bearing a boronic acid protecting group, or a group capable of being hydrolyzed to a hydroxyl group in an aqueous solution at physiological pH or in biological fluids; and the wavy lines at asymmetric carbons Ca and Cb, which independently indicate for each asymmetric carbon an R configuration, an S configuration, or a mixture of both configurations such that all stereoisomers and all stereomeric mixtures are included. Also included within the scope of the pyrrolidine compounds of the invention are cyclic isomers thereof, any pharmaceutically acceptable salts thereof, any prodrugs thereof, and any solvates thereof.
The pyrrolidine compounds of the invention may exist in either of two forms, the linear form represented by formula I above and the cyclic isomer form represented by formula V below.




The cyclic isomer form and the linear form are in thermodynamic equilibrium when in solution. The equilibrium shifts depending upon pH. Thus, the predominance of one form over the other in solution depends upon the pH so that at acidic pH, the linear isomer predominates while at basic pH, the cyclic isomer predominates. The linear and cyclic isomers are also stable such that either form may be isolated as a solid. The isolated cyclic isomer can function as a prodrug.

The invention also is directed to a pharmaceutical composition containing a pyrrolidine compound of the invention and a pharmaceutical carrier. The pharmaceutical composition may be formulated to be dosed by any administrative route including but not limited to parenteral injection, oral, buccal, rectal and the like.
The invention is as well directed to a method of treatment of a malcondition that can be regulated or normalized via inhibition of DPP-IV. The method involves administration of an effective amount of a pyrrolidine compound of the invention, such as would be present in a pharmaceutical composition of the invention, to mammals, especially humans, to affect a malcondition that can be regulated or normalized via inhibition of DPP-IV. Preferably, an effective amount of a pyrrolidine compound of the invention exhibits lower toxicity than do non-selective inhibitors of DPP-IV, particularly in comparison to boronic acid inhibitors of DPP-IV that also display inhibition of other DPP enzymes and FAP. Therefore, the invention is directed to methods for selectively inhibiting DPP-IV including administering to a patient in need of such treatment a therapeutically effective amount of a pyrrolidine compound of the invention.
The invention further is directed to pharmaceutical combinations of a pyrrolidine compound of the invention and one or more other medicaments that are useful for treatment of a malcondition that can be regulated or normalized via inhibition of DPP-IV. Such malconditions are associated with impairments in glycemic control especially Diabetes Mellitus and related conditions. These pharmaceutical combinations may be formulated according to the invention as pharmaceutical compositions.
The invention is also directed to a process for preparing a pyrrolidine compound of the invention, a method for preparing a pharmaceutical composition of the invention, and the use of a pyrrolidine compound of the invention in a method for the preparation of a medicament for treating a malcondition that can be regulated or normalized via inhibition of DPP-IV.
DEFINITIONS
The term “absolute configuration” in connection with an asymmetric carbon is determined by considering the tetrahedral shape of the asymmetric carbon bonds, assigning a priority of 1 through 4 to each of the groups bound to the asymmetric carbon with the group having the highest atomic number having the first priority. If the tetrahedron is viewed from a side remote from group 4, an R absolute configuration is assigned when groups 1-3 are in a clockwise arrangement and an S absolute configuration is assigned when groups 1-3 are in a counterclockwise arrangement.
The term “asymmetric carbon” means a carbon atom covalently bound to four different groups.
The term “beta cell degeneration” is intended to mean loss of beta cell function, beta cell dysfunction, and death of beta cells, such as necrosis or apoptosis of beta cells.
The term “Diabetes Mellitus and related conditions” refers to Type 1 diabetes, Type 2 diabetes, gestational diabetes, MODY, impaired glucose tolerance, impaired fasting glucose, hyperglycemia, impaired glucose metabolism, insulin resistance, obesity, diabetic complications, and the like.
The term “diabetic complications” refers to conditions, diseases and maladies associated with diabetes including retinopathies, neuropathies, nephropathies, cardiomyopathies, dermopathies, arthrosclerosis, coronary artery disease and other known complications of diabetes.
The term “diastereomer” means one member of a group of two or more stereoisomers having at least two asymmetric carbons such that these stereoisomers are not mirror images of each other.
The terms “DPP-VII, DPP-VIII, DPP-IX and FAP” mean respectively amino dipeptidyl peptidase VII, VIII, IX and fibroblast activation protein. The DPP enzymes cleave dipeptide moieties at the N-terminus of their protein or oligopeptide substrates. In particular, the term “DPP-IV” denotes dipeptidyl peptidase IV (EC 3.4.14.5; DPP-IV), also known as “CD-26.” DPP-IV preferentially cleaves a dipeptide from the N terminus of a polypeptide chain containing a proline or alanine residue in the penultimate position.
The term “enantiomer” means one member of a pair of stereoisomers having the same molecular structure and at least one asymmetric carbon such that the stereoisomers of the pair are the mirror images of each other. If the enantiomer contains two or more asymmetric carbons, the enantiomeric pair will have opposing asymmetry at each asymmetric carbon.
The term “group that can be hydrolyzed to a hydroxyl” as used herein refers to an ester group formed from the combination of an aliphatic or aromatic alcohol or diol and a boronic acid.
The term “inhibitor” (and its corresponding verb and gerund) means a compound that will reversibly, irreversibly or temporarily interact with an enzyme so as to reduce, modify, slow down or block its enzymatic activity upon its normal substrate. The interaction may occur within or at the enzymatic site or at an allosteric site associated with the enzyme.
The term “N-protecting group” or “N-protected” as used herein refers to those groups intended to protect the N-terminus of an amino acid or peptide or to protect an amino group against undesirable reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in T. W. Greene, P. G. Wuts, “Protective Groups In Organic Synthesis, 3rd Ed.” (John Wiley & Sons, New York (1999)), which is hereby incorporated by reference. N-protecting groups include acyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, α-chlorobutyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and the like; sulfonyl groups such as benzenesulfonyl, p-toluenesulfonyl and the like; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyloxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyloxycarbonyl, 1-(p-biphenylyl)-1-methylethoxycarbonyl, α,α-di methyl-3,5-dimethoxybenzyloxycarbonyl, benzhydryloxycarbonyl, t-butyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2,-trichloroethoxycarbonyl, phenoxycarbonyl, 4-nitrophenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl and the like; alkyl groups such as benzyl, triphenylmethyl, benzyloxymethyl and the like; and silyl groups such as trimethylsilyl and the like. Preferred N-protecting groups are formyl, acetyl, benzoyl, pivaloyl, t-butylacetyl, phenylsulfonyl, benzyl, 9-fluorenylmethyloxycarbonyl (Fmoc), t-butyloxycarbonyl (Boc) and benzyloxycarbonyl (Cbz).
The term “optically active” means an organic compound containing at least one asymmetric carbon such that a solution of the organic compound will rotate plane polarized light.
The term “optically active mixture” means a mixture of optically active compounds in solution that will rotate plane polarized light. The optically active mixture may be a mixture of diastereomers or an unequal mixture of enantiomers.
The term “pharmaceutical salt” means a salt with an inorganic base, organic base (including basic amino acids), inorganic acid, and organic acid (including acidic amino acids). Included as examples of inorganic bases are alkali metals such as lithium, sodium or potassium; alkaline earth metals such as calcium and magnesium or aluminum; and ammonia. Included as examples of organic bases are trimethylamine, triethylamine, pyridine, picoline, ethanolamine, diethanolamine, and triethanolamine. Included as examples of inorganic acids are the instant invention includes, for example, hydro-halogen acids such as hydrochloric acid, hydroboric acid, nitric acid, sulfuric acid, and phosphoric acid. Included as examples of organic acids are mono, di and tri carboxylic or sulfonic acids of 1 to 20 carbons, optionally containing 1 to 6 hydroxyl groups. Included as examples of basic amino acids are arginine, lysine and ornithine. Included as examples of acidic amino acids are aspartic acid and glutamic acid. Further examples of pharmaceutically acceptable salts include the pharmaceutically acceptable salts listed in Journal of Pharmaceutical Science, 66, 2 (1977) which are known to the skilled artisan.
The term “prodrug” means a pharmaceutically acceptable compound that will convert to the active ingredient or an active metabolite thereof upon administration of the prodrug to a living organism, preferably a mammal, more preferably a human. The conversion may occur by enzymatic action, chemical hydrolysis, oxidation, reduction or any other in vivo physiological process for chemical or biochemical reaction.
The term “racemic mixture” means an enantiomeric pair of equal proportions such that they cancel each other's rotation of plane polarized light.
A singular term such as “a pyrrolidine compound of the invention” includes the plural such as the various methyl and ethyl species of the pyrrolidine compounds of the invention as well as mixtures thereof. A plural term such as “pyrrolidine compounds of the invention” includes the individual species as well as the plural indicated by this term, and also mixtures thereof.
The term “selectivity ratio” refers to the IC50 value generated in a biochemical assay measuring inhibition of DPP-IV compared to the IC50 value generated in a biochemical assay measuring inhibition of another DPP family member (e.g. DPP-VII, DPP-VIII, DPP-IX or FAP) whereby the ratio is obtained by dividing the IC50 value of DPP-VII, DPP-VIII, DPP-IX or FAP by the IC50 value for DPP-IV.
The term “solvate” means a solid, crystalline form of a compound which also incorporates molecules of a solvent into the crystal structure. Organic solvents as well as water are included. Another description of a water solvate is a hydrate or hydrated form.
The term “stereoisomer” means one of the absolute configurations of a single organic molecule having at least one asymmetric carbon. Included within the definition of a stereoisomer are enantiomers and diastereomers. One stereoisomer has one absolute configuration about each of the asymmetric carbons of the organic molecule. An organic molecule with one asymmetric carbon presents two stereoisomers. An organic molecule with two asymmetric carbons presents four stereoisomers. An organic molecule with three asymmetric carbons presents eight stereoisomers. Projecting plane polarized light through a solution containing one stereoisomer will cause rotation of the polarized plane.
The term “stereomeric mixture” means a mixture of two or more stereoisomers and includes enantiomers, diastereomers and combinations thereof. The stereomeric mixture may or may not be optically active.
The term “stereomeric purity” at a given percentage means that the designated stereoisomer predominates at that given percentage in a mixture of stereoisomers.
Unless otherwise specifically stated, the definitions of terms for chemical groups, functional groups, moieties and chemical reactions described herein follow the definitions provided in such organic chemistry textbooks and treatises as “Basic Principles of Organic Chemistry”, Roberts and Caserio, W.A. Benjamin & Co. New York, N.Y., 1965; “Advanced Organic Chemistry”, 4th edition, Jerry March, Wiley Interscience, New York, N.Y. 1992; T. W. Greene, P. G. Wuts, “Protective Groups In Organic Synthesis, 3rd Ed.” (John Wiley & Sons, New York (1999)), and Hawley's Condensed Chemical Dictionary, 11th Ed., Sax and Lewis, Van Nostrand, Reinhold, New York, N.Y., 1987. Moreover, the definitions for stereochemical terms are based upon “Stereochemistry of Carbon Compounds”, Ernest Eliel, McGraw-Hill publisher, New York, N.Y. 1962. The disclosures of these text books are incorporated herein by reference.


DETAILED DESCRIPTION OF THE INVENTION
The methyl and ethyl pyrrolidinylaminoacetyl pyrrolidine boronic acid compounds of the invention (pyrrolidine compounds of the invention) have the linear and cyclic isomer structures depicted by formulas I and V above. All stereoisomers, stereomeric mixtures, pharmaceutically acceptable salts, solvates and prodrugs are included as embodiments of the “pyrrolidine compounds of the invention,” (which is also termed “pyrrolidine compound” or “pyrrolidine compounds” herein). Pharmaceutical compositions including a pyrrolidine compound of the invention and a pharmaceutically acceptable pharmaceutical carrier are an additional aspect of the invention. Also included are pharmaceutical combinations of a pyrrolidine compound of the invention and a second medicament known to be useful in the treatment of malconditions characterized by impaired glycemic control, especially Diabetes Mellitus and related conditions. These pharmaceutical combinations may be formulated as pharmaceutical compositions of the pyrrolidine compound, the second medicament and a pharmaceutically acceptable carrier.
The embodiments of the pyrrolidine compounds of the invention include the borate esters and boronic acid groups such that R2 and R3 of formulas I and V, independently or together, are —OH, —O−M+ wherein M+ is a cation, a hydroxyl bearing a boronic acid protecting group, or a group capable of being hydrolyzed to a hydroxyl group in an aqueous solution at physiological pH or in biological fluids. When R2 and R3 are groups that can be hydrolyzed to hydroxyls, they may be formed from mono-alcohols and diols of 1 to 15 carbons and the alcohols and diols may be linear, branched, cyclic or aromatic, and may optionally be substituted with ester and/or amide groups. Examples include (+)-pinanediol; pinacol; 1,2-dicyclohexyl-ethanediol; 1,2-ethanediol; 2,2-diethanolamine; 1,3-propanediol; 2,3-butanediol, diisopropyl tartrate; 1,4-butanediol; diisopropylethanediol; (S,S,)-5,6-decanediol; 1,1,2-triphenyl-1,2-ethanediol; (2R,3R)-1,4-dimethoxy-1,1,4,4-tetraphenyl-2,3-butanediol; methanol; ethanol; isopropanol; catechol; or 1-butanol.
Surprisingly, it has been discovered that the pyrrolidine compounds of the invention display selectivity for DPP-IV relative to other dipeptidyl peptidase enzymes. By selectivity for DPP-IV it is meant that the pyrrolidine compounds of the invention more strongly inhibit DPP-IV, than at least one closely related enzyme such as DPP-VII, DPP-VIII, DPP-IX and FAP. While not wishing to be bound by any theory, it is believed that this unexpected selectivity for DPP-IV results in an improved therapeutic profile with diminished side-effects for the pyrrolidine compounds of the invention compared with other boronic acid inhibitors and compared to any other non-selective DPP-IV inhibitor. In particular it is believed that potent inhibition of DPP-VIII by other inhibitors correlates with the acute toxicity observed in animal studies. As detailed in the following Examples section, administering a compound with DPP-IV and DPP-VIII inhibitory activity to dogs leads to severe emesis and diarrhea. The same compounds administered to some strains of rats resulted in death. The pyrrolidine compounds of the invention avoid significant inhibition of DPP-VIII and therefore avoid the toxicities observed in both dog and rat.
Synthesis of the Pyrrolidine Compounds of the Invention
The invention also relates to processes for preparing a pyrrolidine compounds of the invention. As shown below and as described in the examples, the pyrrolidine compounds of the invention are prepared by reacting a pyrrolidine, suitably protected with a standard protecting group such as Boc-, Fmoc-, Cbz- or the like, with sec-BuLi/TMEDA followed by a boron source such as B(OCH3)3, to provide the boronic ester derivative. Acid hydrolysis of the methyl esters with HCl provides the boronic acid intermediate 1. Reaction of 1 with (+) pinanediol, deprotection of the amino protecting group, and recrystallization provides the pinanediol ester 2 as an isomerically pure salt.
Intermediate 2 is useful for the synthesis of the pyrrolidine compounds of the invention. For example, N-acylation of 2 with chloroacetyl chloride provides the α-chloro amide 3. Treatment of 3 with Na2CO3 and a pyrrolidinylamine, hydrolysis of the pinanediol boronic ester, and N-deprotection provides a pyrrolidine compound of the invention, 4.




wherein R1 is methyl or ethyl. The S stereoisomer of 2 or a mixture of stereoisomers can be obtained by appropriate manipulation of the recrystallization step, which includes a resolution using an optically active pinanediol. Use of an R or S stereoisomer of the amino pyrrolidine reagent for reaction with 3, or use of a stereomeric mixture thereof will yield the desired stereochemistry at the Cb asymmetric carbon as shown in structure 4.

Thus, another aspect of the invention provides a process for preparing a pyrrolidine compound of the invention including coupling a reactive compound of Formula II:




wherein L is a leaving group such as a halogen, mesylate, tosylate, triflate or the like; and R2 and R3 independently or together are —OH, —O−M+ wherein M+ is a cation, a hydroxyl bearing a boronic acid protecting group, or a group capable of being hydrolyzed to a hydroxyl group in an aqueous solution at physiological pH or in biological fluids;

with a compound of Formula III





wherein Pr is an N-protecting group such as Boc, Cbz, Fmoc, benzyl or the like; and R1 is methyl or ethyl; to provide an ester derivative of a pyrrolidine compound of the invention. The resulting boronic ester derivative of a pyrrolidine compound of the invention may be deprotected to remove Pr and to recover the pyrrolidine compound of the invention as an ester, a free acid or as a salt. In some embodiments, L is halogen, including but not limited to Cl. In others R2 and R3 are each methoxy or together are pinanedioxy, e.g. (+)-pinanedioxy as in compound 3 above. In still others, Pr is Boc.

An alternative synthesis of the pyrrolidine compounds of the invention is provided in U.S. patent application Ser. No. 60/704,380, filed Aug. 1, 2005 and entitled “Methods of Preparing Heterocyclic Boronic Acids and Derivatives Thereof.”
The pyrrolidine compounds of the invention may be formed as pharmaceutically acceptable salts. The pharmaceutical salts may be obtained as the direct products of compound synthesis. In the alternative, the free base may be dissolved in a suitable solvent containing the appropriate acid (or vice versa), and the salt isolated by evaporating the solvent or otherwise separating the salt and solvent. The processes for forming pharmaceutically acceptable salts from amine compounds such as the pyrrolidine compounds of the invention are well-known in the art. See, for example, “The Practice of Medicinal Chemistry, Second Edition”, by Camille G. Wermuth, Academic Press, New York, N.Y., 1996.
The pyrrolidine compounds of the invention may form solvates with standard low molecular weight solvents, including water to yield hydrates, using methods known to the skilled artisan. These processes for forming solvates are also well-known in the art. See, for example “The Practice of Medicinal Chemistry” cited above.
It is to be understood that the invention extends to all of the stereoisomers of the pyrrolidine compounds, including enantiomers, diastereomers, as well as the racemates and stereoisomeric mixtures. The mixtures may or may not be optically active.
In some embodiments, the pyrrolidine compounds of the invention may have an optical purity of at least about 55%, preferably 80%, more preferably 90 wt %, most preferably 98% or more of a single stereoisomer. In other embodiments, the pyrrolidine compounds are optically enriched enantiomers. In still other embodiments, the pyrrolidine compounds are mixtures of stereoisomers including but not limited to unequal mixtures of enantiomers and/or mixtures of diastereomers.
Methods/Uses of the Pyrrolidine Compounds of the Invention
The methods of treatment and use of the pyrrolidine compounds of the invention are based upon inhibition of dipeptidyl peptidase-IV by contact of the enzyme, dipeptidyl peptidase-IV, with a pyrrolidine compound in any of its form as described above. The contact may be accomplished in vitro such as through a diagnostic test or a screening test, or in vivo through an appropriate administrative route as discussed below.
The in vivo methods according to the invention involve a pyrrolidine compound of the invention in its role as a selective inhibitor of DPP-IV. For example, the invention provides a method of treatment of a mammal (such as a human) suffering from a malcondition that can be regulated or normalized via inhibition of DPP-IV such as any malcondition characterized by impaired glycemic control, especially Diabetes Mellitus and related conditions by administering an effective amount of a pyrrolidine compound of the invention to treat, control, ameliorate or prevent the malcondition. These malconditions are known to be the result, at least in part, of the presence, or altered activity, of peptides regulated by the enzyme DPP-IV, especially in the context of its physiological role in glycemic control. These methods of the invention are accomplished by administering to the mammal (e.g., a human) an effective amount of a pyrrolidine compound of the invention. Treatment is affected by inhibition of DPP-IV. Administration is typically accomplished through use of a pharmaceutical composition containing a pyrrolidine compound of the invention.
The method of the invention further includes a method for selectively inhibiting DPP-IV over related enzymes. In some embodiments of the methods for treatment, DPP-IV is inhibited by greater than 5-fold relative to one or more other dipeptidyl peptidases. In other embodiments, DPP-IV is inhibited by greater than 10-, 20-, or even 50-fold or more over other dipeptidyl peptidases. Exemplary other dipeptidyl peptidases include DPP-VII, DPP-VIII, DPP-IX, and FAP. For example, the pyrrolidine compounds of the invention can selectively inhibit DPP-IV over dipeptidyl peptidase-VII, or DPP-IV over dipeptidyl peptidase-VIII, or DPP-IV over dipeptidyl peptidase-IX, or DPP-IV over fibroblast activation protein (FAP). In additional embodiments, the pyrrolidine compounds of the invention selectively inhibit DPP-IV over dipeptidyl peptidase-VIII and fibroblast activation protein. In other embodiments, the pyrrolidine compounds of the invention selectively inhibit DPP-IV over dipeptidyl peptidase-VII, dipeptidyl peptidase-VIII, and fibroblast activation protein. This selectivity applies to in vitro and to in vivo situations. In particular, it has been determined in an in vivo protocol study in humans that pyrrolidine compounds of the invention maintained selectivity for inhibition of DPP-IV over the other amino dipeptidyl peptidases. Preferably, the DPP-IV selectivity is shown relative to DPP-VIII.
For in vivo use as a DPP-IV inhibitor, the pyrrolidine compounds of the invention may be formulated in any manner as described herein and administered in an effective amount to a patient (human) suffering from a malcondition that can be regulated or normalized by inhibition of DPP-IV, especially a malcondition characterized by impaired glycemic control, especially Diabetes Mellitus and related conditions. For example, the malcondition can be Type 1 diabetes, Type 2 diabetes, gestational diabetes, MODY, impaired glucose tolerance, impaired fasting glucose, hyperglycemia, impaired glucose metabolism, impaired glucose tolerance (IGT) and its progression to Type II diabetes, hyperinsulinemia, obesity, beta cell degeneration (in particular apoptosis of beta cells), the progression of non-insulin-requiring Type II diabetes to insulin requiring Type II diabetes; loss of the number and/or the size of beta cells in a mammalian subject, and diabetic complications such as retinopathy, neuropathy, nephropathy, cardiomyopathy, dermopathy, diabetes related infection, atherosclerosis, coronary artery disease, stroke and similar malconditions.
In other embodiments of method of treatment according to the invention, insulin resistance is a component of the malcondition that can be regulated or normalized by inhibition of DPP-IV. For example, the malconditions can be impaired fasting glucose, impaired glucose tolerance, polycystic ovarian syndrome and the like. In yet other embodiments, the malcondition that can be regulated or normalized by inhibition of DPP-IV involves a decrease of islet neogenesis, fl-cell survival, or insulin biosynthesis.
The administered dose of the pyrrolidine compounds of the invention will be carefully adjusted according to age, weight and condition of the patient, as well as the route of administration, dosage form and regimen and the desired result. The ultimate choice of dosage, route and pharmaceutical formulation will determined by the patient's attending physician, whose wisdom and judgment will guide this process. The dose for adults may range from about 0.5 to about 2,000 mg per day, preferably about 10 mg to about 1000 mg per day, more preferably about 50 mg to about 750 mg per day which can be administered in a single dose or in the form of multiple doses given up to 4 times per day.
The use of pyrrolidine compounds of the invention also includes the manufacture of a medicine and a method of treatment using such a medicine in the form of a pharmaceutical composition.
Pharmaceutical Combinations and their Use in Treatment
The pyrrolidine compounds of the invention may be combined with a second medicament to form a pharmaceutical combination of the invention. The second medicament is a known agent for treating, controlling, or preventing a malcondition that can be regulated or normalized via inhibition of DPP-IV. The malconditions treated by such combinations are those that can be regulated or normalized via inhibition of DPP-IV and thus are the same as those described above in connection with sole treatment using the pyrrolidine compounds of the invention.
The second medicament may include a therapeutically effective amount of a dipeptidyl peptidase-IV inhibitor other than the pyrrolidine compounds of the invention. The second medicament preferably may be a known anti-diabetic agent including but not limited to an agent that increases insulin secretion, an agent that increases insulin sensitivity, an agent that reduces the uptake of sugar from the gastrointestinal track, an agent that enhances the effect of endogenous peptides or proteins that play a role in glycemic control, or an agent that acts a replacement therapy for endogenous peptides or proteins that have a known role in glycemic control. Such agents include but are not limited to glyburide (e.g. Micronase and Diabeta), glipizide (e.g. Glucotrol), nateglinide (e.g. Starlix), repaglinide (e.g. Prandin), metformin (e.g. Glucophage), rosiglitazone (e.g. Avandia), acarbose (e.g. Precose), miglitol (e.g. Glyset), exenatide (e.g. Byetta), and insulin (e.g. Humulin and Novolin). Additional exemplary agents include but are not limited to biguanides, chlorpropamide, a glucagon-like peptide-1 (GLP-1) or mimetic thereof such as LY315902 or LY307161, glimepiride, meglitinide, phenformin, pioglitazone, sulfonyl ureas, troglitazone, G1-262570, isaglitazone, JTT-501, NN-2344, L895645, YM-440, R-119702, AJ9677, KAD1129, APR-HO39242, GW-409544, KRP297, AC2993, Exendin-4, and NN2211. The chemical structures, trivial names and pharmacological studies of the foregoing compounds designated by letters and numbers are readily available from the web, for example, by entering the letter/number designation as a search term in the GOOGLE search web site.
Pyrrolidine compounds of the invention may be used in combination with one or more second medicaments useful as antidiabetic agents (employed to treat diabetes and related diseases). The second medicament may be administered orally in the same dosage with the pyrrolidine compounds of the invention, or in a separate oral dosage form. The pyrrolidine compounds of the invention and the second medicament may also be administered, for example by injection, separately, simultaneously or as a mixture.
The pharmaceutical combination of the invention can be formulated as a pharmaceutical composition of a pharmaceutically acceptable carrier along with the pyrrolidine compounds of the invention and one or more second medicaments.
In the pharmaceutical combination of the invention, the pyrrolidine compounds of the invention are typically present in a weight ratio to the second medicament of from about 0.01:1 to about 100:1, or preferentially from about 0.1:1 to about 5:1.
The use of the pyrrolidine compounds of the invention in combination with one or more other antidiabetic agents may produce antihyperglycemic results greater than that possible from each of these antidiabetic agents alone. The use of these pyrrolidine compounds of the invention in combination with one or more other antidiabetic agents may also produce a synergistic effect in that the antihyperglycemic result may be greater than the combined additive antihyperglycemic effects produced by these antidiabetic agents.
The effective amount of a second medicament formulated as a component of the pharmaceutical combination of the invention will follow the recommendations of the second medicament manufacturer, the judgment of the attending physician and will be guided by the protocols and administrative factors for amounts and dosing as indicated in the PHYSICIAN'S DESK REFERENCE (PDR).
The administered dose of a pyrrolidine compound of the invention within the pharmaceutical combination will be carefully adjusted according to age, weight and condition of the patient, as well as the route of administration, dosage form and regimen and the desired result. The ultimate choice of dosage, route and pharmaceutical formulation will determined by the patient's attending physician, whose wisdom and judgment will guide this process.
The compositions described above may be administered in the dosage forms as described above in single or divided doses of one to four times daily. It may be advisable to start a patient on a low dose combination and work up gradually to a high dose combination.
In a further embodiment of the pharmaceutical combinations of the invention, the second medicament can be known anti-obesity agents including but not limited to a beta 3 adrenergic agonist, a lipase inhibitor, a serotonin (and dopamine) reuptake inhibitor, a thyroid hormone receptor-beta agonist, an anorectic agent, a fatty acid oxidation upregulator, or a mixture of any two or more thereof. Suitable, known anti-obesity agents include orlistat, sibutramine, topiramate, axokine, dexamphetamine, phentermine, phenylpropanolamine, famoxin, mazindol, or a mixture of any two or more thereof. These anti-obesity agents may be employed in the same dosage form with a pyrrolidine compound of the invention or in different dosage forms, and the administrative dosages and regimens applied to the anti-obesity agents will follow the recommendations and guides generally known in the art and/or set out in the PDR.
In another embodiment of the pharmaceutical combinations of the invention, the second medicament may be an agent for treating polycystic ovary syndrome. The agent for polycystic ovary syndrome which may be optionally employed in combination with a pyrrolidine compound of the invention may be 1, 2, or more of gonadotropin releasing hormones (GnRH), leuprolide (Lupron®), Clomid®, Parlodel®, oral contraceptives or insulin sensitizers such as PPAR agonists, or other conventional agents for such use which may be employed in amounts specified in the PDR.
Pharmaceutical Compositions of the Invention
The invention includes a pharmaceutical composition containing a pyrrolidine compound of the invention, with or without another medicament as described above, in association with a pharmaceutical carrier. The pharmaceutical composition can be formulated with one or more carriers such as conventional solid or liquid vehicles or diluents and pharmaceutical additives of a type appropriate to the mode of desired administration. A pyrrolidine compound of the invention in a pharmaceutical composition can be administered to mammalian species, especially humans, an oral, buccal, rectal, pulmonary or similar route, for example, in the form of tablets, capsules, granules or powders. It can be administered by a parenteral route in the form of injectable preparations. It can be administered by a transdermal route either by a release patch for transdermal delivery or by electro-transport using an appropriate delivery device.
Pharmaceutical compositions containing a pyrrolidine compound of the invention of the invention may be prepared by conventional techniques, e.g. as described in Remington: The Science and Practice of Pharmacy, 19th Ed., 1995. The compositions may appear in conventional forms, for example capsules, tablets, aerosols, solutions, suspensions or topical applications.
Typical pharmaceutical compositions include a pyrrolidine compound of the invention formulated with a pharmaceutically acceptable carrier which may be an excipient or a diluent, or may be enclosed within a carrier which can be in the form of a capsule, sachet, tablet, paper or other container. In making the compositions, conventional techniques for the preparation of pharmaceutical compositions may be used.
For example, the pyrrolidine compounds of the invention will usually be mixed with a carrier, or diluted by a carrier, or enclosed within a carrier which may be in the form of an ampoule, capsule, tablet, sachet, paper, or other container. When the carrier serves as a diluent, it may be solid, semi-solid, or liquid material that acts as a vehicle, excipient, or medium for the active compounds. The pyrrolidine compounds can be adsorbed on a granular solid container for example in a sachet. Some examples of suitable carriers are water, salt solutions, alcohols, polyethylene glycols, polyhydroxyethoxylated castor oil, peanut oil, olive oil, gelatin, lactose, terra alba, sucrose, dextrin, magnesium carbonate, sugar, cyclodextrin, amylose, magnesium stearate, talc, gelatin, agar, pectin, acacia, stearic acid or lower alkyl ethers of cellulose, silicic acid, fatty acids, fatty acid amines, fatty acid monoglycerides and diglycerides, pentaerythritol fatty acid esters, polyoxyethylene, hydroxymethylcellulose and polyvinylpyrrolidone. Similarly, the carrier or diluent may include any sustained release material known in the art, such as glyceryl monostearate or glyceryl distearate, alone or mixed with a wax.
The formulations can be mixed with auxiliary agents which do not deleteriously react with the pyrrolidine compounds. Such additives can include wetting agents, emulsifying and suspending agents, salt for influencing osmotic pressure, buffers and/or coloring substances preserving agents, sweetening agents or flavoring agents. The pharmaceutical compositions can also be sterilized if desired.
The route of administration may be any route, which effectively transports the pyrrolidine compounds of the invention to the appropriate or desired site of action, such as oral, nasal, pulmonary, buccal, rectal, subdermal, intradermal, transdermal or depot, subcutaneous, intravenous, intraurethral, intramuscular, intranasal, ophthalmic solution or an ointment, the oral route being preferred.
If a solid carrier is used for oral administration, the preparation may be tabletted, placed in a hard gelatin capsule in powder or pellet form or it can be in the form of a troche or lozenge. If a liquid carrier is used, the preparation may be in the form of a syrup, emulsion, soft gelatin capsule or sterile injectable liquid such as an aqueous or non-aqueous liquid suspension or solution.
Injectable dosage forms generally include aqueous suspensions or oil suspensions which may be prepared using a suitable dispersant or wetting agent and a suspending agent. Injectable forms may be in solution phase or in the form of a suspension, which is prepared with a solvent or diluent. Acceptable solvents or vehicles include sterilized water, Ringer's solution, or an isotonic aqueous saline solution. Alternatively, sterile oils may be employed as solvents or suspending agents. Preferably, the oil or fatty acid is non-volatile, including natural or synthetic oils, fatty acids, mono-, di- or tri-glycerides.
For injection, the pharmaceutical composition may be a powder suitable for reconstitution with an appropriate solution as described above. Examples of these include, but are not limited to, freeze dried, rotary dried or spray dried powders, amorphous powders, granules, precipitates, or particulates. For injection, the pharmaceutical composition may optionally contain stabilizers, pH modifiers, surfactants, bioavailability modifiers and combinations of these. The pyrrolidine compounds of the invention may be formulated for parenteral administration by injection such as by bolus injection or continuous infusion. A unit dosage form for injection may be in ampoules or in multi-dose containers.
The pharmaceutical composition of the invention may be designed to provide quick, sustained, or delayed release of the active ingredient after administration to the patient by employing procedures well known in the art. Thus, the pharmaceutical composition may also be formulated for controlled release or for slow release.
The pharmaceutical composition of the invention may include, for example, micelles or liposomes, or some other encapsulated form, or may be administered in an extended release form or an enteric coated form to provide a prolonged storage and/or delivery effect. Therefore, the pharmaceutical composition may be compressed into pellets or cylinders and implanted intramuscularly or subcutaneously as depot injections or as implants such as stents. Such implants may employ known inert materials such as silicones and biodegradable polymers, e.g., polylactide-polyglycolide. Examples of other biodegradable polymers include poly (orthoesters) and poly(anhydrides).
The pyrrolidine compounds of the invention may be formulated as a sustained release implant or implantable material suitable for continuous administration over a significant period of time. Typical sustained release implants are formed from polymers of pharmaceutically acceptable, biodegradable polymers such as polymers and copolymers of lactic acid, lactide, glycolic acid, glycolide, caproic acid and caprolactone. The dose and amount of a pyrrolidine compound of the invention within the implant will be calculated to deliver the desired single dose blood level of pyrrolidine compound.
For nasal administration, the pharmaceutical composition may contain a pyrrolidine compound of the invention dissolved or suspended in a liquid carrier, in particular an aqueous carrier, for aerosol application. The carrier may contain additives such as solubilizing agents, e.g., propylene glycol, surfactants, absorption enhancers such as lecithin (phosphatidylcholine) or cyclodextrin, or preservatives such as parabenes.
For parenteral application, particularly suitable are injectable solutions or suspensions, preferably aqueous solutions with a pyrrolidine compound of the invention dissolved in polyhydroxylated castor oil.
Tablets, dragees, or capsules having talc and/or a carbohydrate carrier or binder or the like are particularly suitable for oral application. Preferable carriers for tablets, dragees, or capsules include lactose, corn starch, and/or potato starch. A syrup or elixir can be used in cases where a sweetened vehicle can be employed.
A typical tablet that may be prepared by conventional tabletting techniques may contain:
















Core:




Pyrrolidine compound of the invention*
300 mg 



Colloidal silicon dioxide (Aerosil) ®
1.5 mg 



Cellulose, microcryst. (Avicel) ®
70 mg



Modified cellulose gum (Ac-Di-Sol) ®
7.5 mg 



Magnesium stearate
Adde



Coating:



HPMC approx.
  9 mg



**Mywacett 9-40 T approx.
0.9 mg 







*Pyrrolidine compound is formulated as free compound or salt thereof.



**Acylated monoglyceride used as plasticizer for film coating.






A typical capsule for oral administration contains a pyrrolidine compound of the invention (250 mg), lactose (75 mg) and magnesium stearate (15 mg). The mixture is passed through a 60 mesh sieve and packed into a No. 1 gelatin capsule. A typical injectable preparation is produced by aseptically placing 250 mg of pyrrolidine compound of the invention into a vial, aseptically freeze-drying and sealing. For use, the contents of the vial are mixed with 2 mL of physiological saline, to produce an injectable preparation.
The pyrrolidine compounds of the invention may be administered to a mammal, especially a human in need of such treatment, prevention, elimination, alleviation or amelioration of the various malconditions mentioned above. Such mammals include also animals, both domestic animals, e.g. household pets, farm animals, and non-domestic animals such as wildlife.
The pyrrolidine compounds of the invention are effective over a wide dosage range. For example, in the treatment of adult humans, dosages from about 0.5 to about 2000 mg, preferably from about 10 mg to about 1000 mg, per day, more preferably about 50 to 750 mg per day may be used. A typical dosage is about 50 mg to about 750 mg per day. In choosing a regimen for patients it may frequently be necessary to begin with a higher dosage and when the condition is under control to reduce the dosage. The exact dosage will depend upon the mode of administration, on the therapy desired, form in which administered, the subject to be treated and the body weight of the subject to be treated, and the preference and experience of the physician or veterinarian in charge.
Generally, the pyrrolidine compounds of the invention are dispensed in unit dosage form including from about 0.5 to about 2000 mg of active ingredient together with a pharmaceutically acceptable carrier per unit dosage.
Usually, dosage forms suitable for oral, nasal, pulmonary or transdermal administration include from about 0.5 mg to about 2000 mg, preferably from about 10 mg to about 1000 mg per day, more preferably from about 50 mg to about 750 mg of the pyrrolidine compounds admixed with a pharmaceutically acceptable carrier or diluent.
The pharmaceutical combinations of the invention may be formulated as pharmaceutical compositions employing all of the embodiments, carriers, route designs and the like described above for formulation of a pharmaceutical composition of a pyrrolidine compound alone.
The invention also encompasses prodrugs of a pyrrolidine compound of the invention which on administration undergo chemical conversion by metabolic processes before becoming active pharmacological substances. In general, such prodrugs will be functional derivatives of a pyrrolidine compound of the invention which are readily convertible in vivo into a pyrrolidine compound of the invention. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in “Design of Prodrugs”, ed. H. Bundgaard, Elsevier, 1985.
The invention also encompasses active metabolites of a pyrrolidine compound of the invention.
Thus, another aspect of the invention provides a pharmaceutical composition of the pyrrolidine compounds of the invention, alone or in combination with another type antidiabetic agent and/or other type therapeutic agent.
Additional embodiments of the invention are represented by:


    
    
        A pharmaceutical composition including a pyrrolidine compound of the invention, as described above, together with at least one pharmaceutically acceptable carrier or diluent;
        Methods of making a pharmaceutical composition of a pyrrolidine compound of the invention wherein the pharmaceutically acceptable carrier or diluent is suitable for oral administration;
        Methods of making a pharmaceutical composition of a pyrrolidine compound of the invention suitable for oral administration further including the step of formulating the composition into a tablet or capsule;
        Methods of making a pharmaceutical composition of a pyrrolidine compound of the invention wherein the pharmaceutically acceptable carrier or diluent is suitable for parenteral administration;
        Methods of making a pharmaceutical composition of a pyrrolidine compound of the invention suitable for parenteral administration further including the step of lyophilizing the composition to form a lyophilized preparation.
    
    


DPP-1V inhibitory activity of the pyrrolidine compounds of the invention may be determined by use of an in vitro assay system. Inhibition constants (Ki or IC50 values) for the DPP-IV inhibitors of the invention may be determined by the method described below.
A further detailed description of the invention is given with reference to the following non-limiting examples.
EXAMPLES
Example 1
Synthesis of Methyl-Substituted Pyrrolidine Boronic Acid Compounds
Unless otherwise noted, materials were obtained from commercial suppliers and used without further purification. 1H NMR spectra were determined on a Bruker Avance 300 spectrometer. Chemical shifts are expressed in ppm downfield from internal tetramethylsilane. Mass spectra were obtained on a Finnigan LCQ mass spectrometer with ESI source.
THF and ethyl ether were distilled from sodium/benzophenone ketyl. Dichloromethane was dried by distillation from phosphorus pentoxide.




Preparation of compound 2: To a solution of 1 (100.0 g, 0.97 mol) in dichloromethane (1000 mL) was added triethyl amine (134 mL, 0.97 mol) dropwise. After being stirred for 30 min, benzaldehyde (102.8 g, 0.97 mol) was added, followed by the addition of MgSO4 (232.0 g, 1.97 mol). The mixture was stirred overnight. After filtration, the filtrate was evaporated to give compound 2 (150.0 g, 81.1%). 1H NMR (CDCl3): δ 1.52 (m, 3H), 3.74 (s, 3H), 4.15-4.17 (m, 1H), 7.53-7.57 (m, 2H), 7.75-7.77 (m, 3H), 8.31 (s, 1H).
Preparation of compound 4: To a solution of t-BuOK (87.4 g, 0.78 mol) in THF (800 mL) at room temperature was added a solution of compound 2 (150.0 g, 0.78 mol) in THF (500 mL) dropwise. After stirring for 1 h, the solution of allyl bromide (94.4 g, 0.78 mol) in THF (400 mL) was added dropwise to the resulting mixture. After stirring overnight, the solvent was removed in vacuo and the residue extracted with ethyl acetate, washed with water and brine, dried over with anhydrous Na2SO4. The solvent was evaporated to give compound 3. To a solution of compound 3 in 450 mL ethyl acetate was added 6N HCl (400 mL). The mixture was stirred for one hour and then extracted with ethyl acetate, the aqueous phase was adjusted to pH 9-10 and extracted with ethyl acetate three times. The combined organic layers were dried and evaporated to afford compound 4 as a brown oil (70 g, 62.7%). 1H NMR (CDCl3): δ1.26 (s, 3H), 2.51-2.61 (m, 1H), 2.63-2.69 (m, 1H), 3.74 (s, 3H), 4.23-4.26 (m, 1H), 5.04-5.26 (m, 1H), 5.54-5.71 (m, 1H); MS: 144 (M+1).
Preparation of compound 5: A solution of 4 (47 g, 0.33 mol) and Boc2O (72 g, 0.33 mol) in THF (300 mL) was heated to reflux overnight. After concentration, the residue was purified by column chromatography on silica gel (PE:EA=10:1) to give compound 5 (50.1 g, 62.6%). 1H NMR (CDCl3): δ1.26 (m, 3H) 1.50 (s, 9H), 2.64-2.68 (m, 1H), 2.70-2.75 (m, 1H), 3.74 (s, 3H), 5.04-5.28 (m, 2H), 5.41-5.48 (br, 1H), 5.54-5.70 (m, 1H); MS: 244 (M+1).
Preparation of compound 6: Through a solution of compound 5 (50.0 g, 0.21 mol) in CH2Cl2 (300 mL) at −78° C. was bubbled ozone until the solution turned gray-blue. Then PPh3 (50.0 g, 0.21 mol) was added slowly to quench the reaction. After concentration, the residue was purified by column chromatography on silica gel (PE:EA=10:1) to give compound 6 (27.1 g, 53.6%). 1H NMR (CDCl3): δ0.84 (m, 3H), 1.51 (s, 9H), 2.91-2.96 (m, 1H,), 3.48-3.61 (m, 1H), 3.74 (s, 3H), 5.61 (br, 1H), 9.70 (s, 1H); MS: 246 (M+1).
Preparation of compound 7: To a solution of compound 6 (10.0 g, 40.8 mmol) in CH2Cl2 (100 mL) was added benzyl amine (4.36 g, 40.8 mmol) and MgSO4 (6.8 g, 57 mmol). After stirring overnight, the resulting mixture was filtered and the filtrate was concentrated to afford compound 7 (12.6 g, 92.6%) as a brown oil. 1H NMR (CDCl3): δ1.21 (t, 3H), 1.51 (s, 9H), 2.81-2.87 (m, 1H,), 2.92-3.01 (m, 1H), 3.67 (s, 3H), 4.10-4.20 (m, 1H), 4.56 (m, 2H), 5.45-5.56 (br, 1H), 7.22-7.31 (m, 5H), 7.76 (br, 1H); MS: 335 (M+1).
Preparation of compound 8: To a solution of compound 7 (12.6 g, 37.7 mmol) in MeOH (200 mL) was added NaBH4 (2.17 g, 45.25 mmol). After stirring 3 h at room temperature, the mixture was poured into water, extracted with ethyl acetate, and the combined organic phases were dried over Na2SO4 and concentrated to afford product 8 (9.1 g, 79.4%) as a brown oil. 1H NMR (CDCl3): δ1.33 (s, 3H), 1.51 (s, 9H), 2.19-2.43 (m, 2H), 3.07-3.24 (m, 2H), 2.02-3.31 (m, 2H), 4.56-4.4 (d, 1H), 4.53-4.58 (d, 1H), 7.22-7.35 (m, 5H); MS: 305 (M+1).
Preparation of compound 9: A solution of 8 (9.1 g, 29.9 mmol) in anhydrous HCl/CH3OH (200 mL) was stirred for 3 h. Then the solvent was evaporated to afford the HCl salt of 9 (6.1 g, 99.8%) as a brown oil. 1H NMR (CDCl3): 1.36 (s, 3H), 2.05-2.20 (m, 2H), 3.14-3.25 (m, 2H), 4.41 (s, 2H), 7.23-7.35 (m, 5H), 8.66 (br, 1H); MS: 205 (M+1).
Preparation of compound 10: A solution of 9 (6.1 g, 29.9 mmol) in THF (20 mL) was added to a solution of LiAlH4 (1.2 g, 32 mmol) in THF (10 mL) at room temperature. After stirring 2 h, water was added to quench this reaction. The resulting mixture was filtered through a pad of silica and the filtrate was evaporated to give crude product which was purified by flash chromatography on silica gel to give compound 10 (3.8 g, 66.9%) as a white solid. 1H NMR (CDCl3): 1.50 (s, 3H), 1.82-1.85 (m, 1H), 2.04-2.13 (m, 1H), 2.39-2.50 (m, 2H), 2.85-2.99 (m, 2H), 3.59-3.70 (m, 2H), 7.20-7.36 (m, 5H); MS: 191 (M+1).
Preparation of compound 11: To a solution of 10 (6.5 g, 34.2 mmol) in CH3OH (50 mL) was added a solution of L-tartaric acid (5.13 g, 34.5 mmol) in CH3OH (50 mL). After being stirred for 2 h at room temperature, the resulting suspension was filtered and re-crystallized five times to get the desired compound 11 (1.1 g, 18.9%). 1H NMR (MeOD): 1.45 (s, 3H), 2.01-2.12 (m, 2H), 2.48 (d, 1H), 2.59-2.68 (m, 1H), 2.95 (d, 1H), 3.07-3.18 (m, 1H), 3.72 (d, 1H), 3.85 (d, 1H), 4.42 (s, 2H), 7.22-7.44 (m, 5H).
Preparation of the compound (+)-12: A solution of 11 (1.1 g, 3.2 mmol) in 10 mL NaOH (2M) was stirred for 2 h at room temperature. The reaction was extracted with ethyl acetate, and the combined organic phases were dried over Na2SO4 and concentrated to afford product (+)-12 (600 mg, 97.7% ee) (Table 1).




Preparation of compound (+)-13: To a solution of compound (+)-12 (100 mg, 0.52 mmol) in anhydrous CH3CN (10 ml) under argon was added Intermediate 1 (213.6 mg, 0.57 mmol) followed by the addition of K2CO3 (358 mg, 2.6 mmol). After stirring overnight, the mixture was filtered and the filtrate was evaporated and purified by p-HPLC to afford (+)-13 (30 mg, 12%). (M+1): 480.
Preparation of compound (+)-14: To the solution of compound (+)-13 (30 mg, 0.06 mmol) in MTBE (2 ml) was added 1N HCl (2 ml), followed by the addition of PhB(OH)2 (9.2 mg, 0.075 mmol). After stirring for 2 h, 10 ml water was added before separation. The aqueous layer was washed with EA and CH2Cl2, and was used directly to the next step. (M+1): 346.
The solution of the above compound in THF (10 ml) was hydrogenated with Pd(OH)2/C at 20 psi H2 After stirring 1 h, the mixture was filtered and the filtrate was lyophilized to afford the (+)-14 (8 mg, 52%). 1H NMR (MeOD): δ1.62 (s, 3H), 1.70-1.75 (m, 1H), 1.93-2.15 (m, 3H), 2.30-2.50 (m, 2H), 3.48-3.58 (m, 3H), 3.60-3.75 (m, 3H), 3.96-4.17 (m, 2H). (M+1): 256.
Preparation of Compound (−)-14: Following similar steps as outlined for compounds 12 to (+)-14 with the exception of using (−)-7. (−)-7 was recovered from the mother liquor and isolated according to the preparation of compounds 33 to 35.
Example 2
Synthesis of Ethyl-Substituted Pyrrolidine Boronic Acid Compounds







Preparation of compound 15: To a solution of 2-amino-butyric acid (50 g, 0.48 mol) in MeOH (300 mL) at 0° C. was added thionyl chloride (66.7 g, 0.57 mol) dropwise. After stirring for 2 h, the reaction was concentrated and the precipitated solid was washed with diethyl ether to afford product 15(63.0 g, 85.1%). 1H NMR (DMSO): δ0.85-0.92 (m, 3H), 1.86-2.0 (m, 2H), 3.73 (s, 3H), 3.91-4.12 (m, 1H), 8.40-8.54 (br, 3H):
Preparation of compound 16: To a solution of 15 (40.9 g, 0.27 mol) in dichloromethane (600 mL) was added dropwise triethyl amine (39 mL, 0.32 mol). After being stirred for 30 min, benzaldehyde (28.3 g, 0.27 mol) was added, followed by the addition of MgSO4 (97.2 g, 0.81 mol). The mixture was stirred overnight. After filtration, the filtrate was evaporated to give compound 16 as a red oil (52.0 g, 94.9%). 1H NMR (CDCl3): δ 0.88 (t, 3H), 1.86-2.0 (m, 2H), 3.68 (s, 3H), 3.83-3.88 (m, 1H), 7.33-7.37 (m, 2H), 7.73-7.76 (m, 3H), 8.23 (s, 1H).
Preparation of compound 18: To a solution of t-BuOK (31.2 g, 0.28 mol) in THF (480 mL) at room temperature was added dropwise a solution of compound 16 (47.1 g, 0.23 mol) in THF (240 mL). After stirring for 1 h, a solution of allyl bromide (33.5 g, 0.28 mol) in THF (120 mL) was added dropwise to the mixture. After stirring overnight, the solvent was removed in vacuo and the residue was extracted with ethyl acetate, washed with water and brine, dried over with anhydrous Na2SO4. The solvent was evaporated to give the compound 17. To the solution of compound 17 in THF (150 mL), was added 6N HCl (200 mL) and the mixture was stirred for one hour. Then extracted with ethyl acetate, the aqueous phase was adjusted to pH=9-10 and extracted with ethyl acetate three times. The combined organic layers were dried and evaporated to afford compound 18 as a brown oil (34.5 g, 95.8%). 1H NMR: (CDCl3): δ0.82 (t, 3H), 1.49-1.51 (m, 1H), 1.61-1.63 (m, 1H), 2.13-3.25 (m, 1H), 2.42-2.59 (m, 1H), 3.68 (s, 3H), 5.17-5.27 (m, 2H), 5.62-5.78 (m, 1H); MS: 158 (M+1).
Preparation of compound 19: A solution of 18 (24.5 g, 0.15 mol) and Boc2O (33 g, 0.15 mol) in THF (300 mL) was heated to reflux overnight. Then the solvent was evaporated and the residue was purified by column chromatography on silica gel (PE:EA=10:1) to give compound 19 (32.1 g, 84.4%) as a brown oil. 1H NMR (CDCl3): δ0.77 (t, 3H) 1.50 (s, 9H), 1.85-1.95 (m, 1H), 2.17-2.29 (m, 1H), 2.44-2.51 (m, 1H), 2.95-3.05 (m, 1H), 3.68 (s, 3H), 5.01-5.06 (m, 2H), 5.38-5.46 (br, 1H), 5.55-5.65 (m, 1H); MS: 258 (M+1).
Preparation of compound 20: Through the solution of compound 19 (32.1 g, 0.12 mol) in dichloromethane (300 mL) at −78° C. was bubbled ozone until the solution turned gray-blue. Then PPh3 (50 g, 0.19 mol) was added slowly to quench the reaction. After concentration, the residue was purified by column chromatography on silica gel (PE:EA=10:1) to give compound 20 (22.1 g, 69.0%). 1H NMR(CDCl3): δ0.78 (t, 3H), 1.51 (s, 9H), 1.65-1.69 (m, 1H,), 2.15-2.35 (m, 1H), 2.91-2.96 (d, 1H,), 3.48-3.61 (m, 1H), 3.72 (s, 3H), 5.61 (br, 1H), 9.62 (s, 1H); MS: 260 (M+1).
Preparation of compound 21: To a solution of compound 20 (5.0 g, 19 mmol) in dichloromethane (50 mL) was added benzylamine (2.2 g, 21 mmol) and anhydrous MgSO4 (6.8 g, 57 mmol). After stirring overnight, the resulting mixture was filtered and the filtrate was concentrated to afford compound 21 as a brown oil (6.7 g, 98.5%). 1H NMR (CDCl3): δ0.78 (t, 3H), 1.51 (s, 9H), 1.65-1.69 (m, 1H,), 2.15-2.35 (m, 1H), 2.86-2.96 (m, 1H), 3.2-3.3 (m, 1H), 3.65 (s, 3H), 4.55 (s, 2H), 5.61 (br, 1H), 7.21-7.41 (m, 5H), 7.31 (s, 1H).
Preparation of compound 22: To a solution of compound 21 (6.7 g, 18.9 mmol) in methanol (420 mL) was added NaBH4 (3.5 g, 92.9 mmol). After stirring 3 h at room temperature, the mixture was poured into water, extracted with ethyl acetate, and the combined organic phases were dried over anhydrous Na2SO4 and concentrated to afford product 22 (5.3 g, 86.6%). 1H NMR (CDCl3): δ0.88-0.93 (t, 3H), 1.43 (s, 9H), 1.65-1.71 (m, 2H,), 2.25-2.55 (m, 2H), 3.10-3.31 (m, 2H), 4.40-4.45 (m, 1H), 4.51-4.62 (m, 1H), 5.2 (br, 1H), 7.23-7.36 (m, 5H); MS: 319 (M+1).
Preparation of compound 23: A solution of 22 (24.5 g, 77 mmol) in anhydrous HCl/CH3OH (100 mL) was stirred for 3 h. Then the solvent was evaporated to afford the HCl salt of 23 (16 g, 95.3%). 1H NMR (CDCl3): 0.82 (t, 3H), 1.52-1.63 (m, 2H), 1.71-1.83 (m, 1H), 2.05-2.10 (m, 1H), 2.20 (s, 2H), 3.01-3.21 (m, 2H), 4.28-4.56 (m, 2H), 7.17-7.35 (m, 5H); MS: 319 (M+1).
Preparation of compound 24: To a solution of LiAlH4 (0.998 g, 26.2 mmol) in THF (10 mL) was added the solution of 23 (2.0 g, 7.87 mmol) in THF (20 ml) at room temperature. After stirring 2 h, water was added. The suspension was filtered through a pad of silica and the filtrate was evaporated. The residue was purified by column chromatography on silica gel (CH2Cl2: CH3OH=10:1) to give compound 24 (0.9 g, 48%). 1H NMR (CDCl3): 0.93-1.23 (t, 3H), 1.64-1.70 (m, 2H), 1.70-1.85 (m, 2H), 2.45-2.54 (m, 2H), 2.71-2.75 (m, 1H), 2.99-3.02 (m, 1H), 3.72 (s, 2H), 7.25-7.35 (m, 5H); MS: 205 (M+1).
Preparation of compound 25: To a solution of 24 (6.0 g, 0.029 mol) in methanol (50 mL) was added the solution of L-tartaric acid (5.2 g, 0.035 mol). After being stirred for 2 h, the resulting suspension was filtered and re-crystallized five times to get the desired compound 25 (2.3 g, 44.8%) as a white solid. 1H NMR (CDCl3): 0.93-1.23 (t, 3H), 1.75-1.82 (m, 2H), 2.01-2.44 (m, 2H), 2.52-2.62 (m, 2H), 2.91-2.96 (d, 1H), 3.14-3.15 (m, 1H), 3.74-3.88 (m, 2H), 4.41 (s, 2H), 7.25-7.35 (m, 5H).
Preparation of the compound (+)-26: A solution of 25 (2.3 g, 9 mmol) in NaOH (2M) was stirred for 2 h at room temperature. The solution was extracted with ethyl acetate and the combined organic phases were dried over anhydrous Na2SO4 and concentrated to afford product (+)-26 (1.0 g, 98.7% ee) as a pale yellow solid.




Preparation of compound (+)-27: To a solution of (+)-26 (1.14 g, 5.5 mmol) in THF (20 mL) at 0° C. was added ethyl bromoacetate (1.09 g, 6.6 mmol) and triethylamine (0.92 mL, 6.6 mmol). After stirring overnight, the precipitate was filtered off and the filtrate was concentrated to afford the crude product (+)-27 (1.61 g, 99%). MS: 291 (M+1).
Preparation of compound (+)-28: To a solution of (+)-27 (1.61 g, 5.5 mmol) in dichloromethane (15 mL) and sat. NaHCO3 (15 mL) was added (Boc)2O (2.40 g, 11.0 mmol) and NaCl (2.0 g, 34 mmol) and heated to reflux overnight. After cooling to room temperature, water was added and the aqueous layer was extracted with chloroform. The combined organic layers were dried over anhydrous sodium sulfate and concentrated to give crude product (+)-28 (1.71 g, 80%). MS: 391 (M+1).
Preparation of compound (+)-29: The solution of (+)-28 (1.71 g, 4.4 mmol), (Boc)2O (1.9 g, 8.8 mmol) and palladium hydroxide on carbon (80 mg) in ethanol (40 mL) was hydrogenated at 40 psi for 3 hours. After the filtration to remove the catalyst, the filtrate was concentrated and the residue was purified by column chromatography on silica gel to give product (+)-29 (1.0 g, 57.0%). MS: 401 (M+1).
Preparation of compound (+)-30: To a solution of (+)-29 (500 mg, 1.25 mmol) in ethanol (10 mL) and water (2 mL) was added lithium hydroxide monohydrate (262 mg, 6.25 mmol) and the reaction was stirred for 2 hours. Then it was adjusted to pH 6 with 10% citric acid. The solution was extracted with ethyl acetate three times. The combined organic layers were dried over anhydrous sodium sulfate and concentrated to give compound (+)-30 (400 mg, 87%). MS: 373 (M+1).
Preparation of compound (+)-31: To a solution of compound (+)-30 (160 mg, 0.43 mmol) in dichloromethane (10 mL) at 0° C. was added N-methyl morpholine (219 μL, 1.57 mmol) and iso-butylchloroformate (70 mg, 0.52 mmol) under Ar atmosphere and stirred for 0.5 hours. Then Intermediate 2 (132 mg, 0.46 mmol) was added and the reaction was stirred for another 2 hours. Dichloromethane and sat. NaCl was added, and after the separation, the organic layer was washed with 6N HCl two times. Then the organic layer was dried over anhydrous sodium sulfate and concentrated to afford product (+)-31 (131 mg, 52%). MS: 590 (M+1).
Preparation of compound (+)-32: To a solution of compound (+)-31 (131 mg, 0.22 mmol) in MTBE (5 mL) and 5N HCl (5 mL) was added phenyl boronic acid (31.7 mg, 0.26 mmol) and stirred for 2 hours. Water was added and the resulting mixture was extracted with EA twice, dichloromethane twice, and then the aqueous layer was lyophilized to afford product (+)-32 (50 mg, 85%) (Table 1). 1H NMR (MeOD): δ1.07 (q, 3H), 1.70-1.80 (m, 1H), 1.95-2.10 (m, 5H), 2.35-2.52 (m, 2H), 3.15-3.23 (m, 1H), 3.44-3.82 (m, 4H), 3.95-4.15 (m, 2H). MS: 270 (M+1).







Preparation of compound 33: The mother liquor of 25 was stirred for 2 h at room temperature in 2N NaOH. The resulting solution was extracted with dichloromethane, and the combined organic phases were dried over anhydrous sodium sulfate and concentrated to afford product 33 (6.7 g).
Preparation of compound 34: To a solution of 33 (6.7 g, 33 mmol) in methanol (60 mL) was added the solution of D-tartaric acid (5.2 g, 0.035 mol). After being stirred for 2 h, the resulting suspension was filtered and re-crystallized five times to get the desired compound 34 (5 g, 87%) as a white solid. 1H NMR (CDCl3): 1.0 (t, 3H), 1.79 (q, 2H), 2.01-2.10 (m, 2H), 2.52-2.62 (m, 2H), 2.95 (d, 1H), 3.11-3.16 (m, 1H), 3.73 (d, 1H), 3.60 (d, 1H), 4.42 (s, 2H), 7.25-7.40 (m, 5H).
Preparation of the compound (−)-35: The solution of 34 (5 g, 19 mmol) in NaOH (2M) was stirred for 2 h at room temperature. The resulting solution was extracted with ethyl acetate, and the combined organic phases were dried over anhydrous sodium sulfate and concentrated to afford product (−)-35 (2.0 g, 52%).
Preparation of compound (−)-36: To a solution of (−)-35 (1.5 g, 7.3 mmol) in THF (50 mL) at 0° C. was added ethyl bromoacetate (1.45 g, 8.7 mmol) and triethyl amine (1.22 mL, 47.1 mmol). After being stirred overnight, the precipitate was filtered out and the filtrate was concentrated to afford the crude product (−)-36 (1.98 g, 94%). MS: 291 (M+1).
Preparation of compound (−)-37: To a solution of (−)-36 (1.98 g, 6.8 mmol) in dichloromethane (25 mL) and sat. NaHCO3 (25 mL) was added (Boc)2O (2.96 g, 13.6 mmol) and NaCl (2.76 g, 47.2 mmol) and heated to reflux overnight. After cooling to room temperature, water was added and the aqueous layer was extracted with chloroform. The combined organic layers were dried over anhydrous sodium sulfate and concentrated to give crude product (−)-37 (2.3 g, 87%). MS: 391 (M+1).
Preparation of compound (−)-38: The solution of (−)-37 (2.3 g, 5.8 mmol), (Boc)2O (2.52 g, 13.6 mmol) and palladium hydroxide on carbon (100 mg) in ethanol (40 mL) was hydrogenated at 40 psi for 3 hours. After the filtration to remove the catalyst, the filtrate was concentrated and the residue was purified by column chromatography on silica gel to give product (−)-38 (1.0 g, 43.0%). MS: 401 (M+1).
Preparation of compound (−)-39: To a solution of (−)-38 (500 mg, 1.25 mmol) in ethanol (10 mL) and water (2 mL) was added lithium hydroxide monohydrate (262 mg, 6.25 mmol) and the reaction was stirred for 2 hours. Then it was adjusted to PH 6 with 10% citric acid and extracted with EA for three times. The combined organic layers were dried over anhydrous sodium sulfate and concentrated to give compound (−)-39 (430 mg, 93%). MS: 373 (M+1).
Preparation of compound (−)-40: To a solution of compound (−)-39 (260 mg, 0.7 mmol) in dichloromethane (20 mL) at 0° C. was added N-methyl morpholine (316 mg, 3.13 mmol) and isobutylchloroformate (118 mg, 0.87 mmol) under Ar atmosphere and stirred for 0.5 hours. Then Intermediate 2 (214.5 mg, 0.75 mmol) was added and stirred for another 2 hours. Dichloromethane and sat. NaCl were added, and after the separation, the organic layer was washed with 6N HCl two times. The organic layer was dried over anhydrous sodium sulfate and concentrated to afford product (−)-40 (230 mg, 56%). MS: 590 (M+1).
Preparation of compound (−)-32: To a solution of compound (−)-40 (115 mg, 0.19 mmol) in MTBE (5 mL) and 5N HCl (5 mL) was added phenyl boronic acid (27.8 mg, 0.22 mmol) and stirred for 2 hours. Water was added and the resulting mixture was extracted with EA twice, dichloromethane twice, and then the aqueous layer was lyophilized to afford product (−)-32 (40 mg, 80%) (Table 1). 1H NMR (MeOD): δ1.05 (q, 3H), 1.90-2.05 (m, 6H), 2.28-2.35 (m, 2H), 3.15-3.20 (m, 1H), 3.44-3.62 (m, 4H), 3.90-4.00 (m, 2H). MS: 270 (M+1).









TABLE 1





Compound




No.
Name
Structure







(+) 14
(2R)-1-{2-[(3S)-Pyrrolidin- 3-methyl-3-ylamino]- acetyl}-pyrrolidine-2- boronic acid






 


(−) 14
(2R)-1-{2-[(3R)-Pyrrolidin- 3-methyl-3-ylamino]- acetyl}-pyrrolidine-2- boronic acid






 


(+) 32
(+)-(2R)-1-{2-[Pyrrolidin- 3-ethyl-3-ylamino]-acetyl}- pyrrolidine-2-boronic acid






 


(−) 32
(−)-(2R)-1-{2-[Pyrrolidin-3- ethyl-3-ylamino]-acetyl}- pyrrolidine-2-boronic acid













Example 3
DPP-IV Inhibitory Assays
The purification of porcine DPP-IV and the enzyme assay under steady state conditions are described in (1) Rahfeld, J. Schutkowski, M., Faust, J., Neubert., Barth, A., and Heins, J. (1991) Biol. Chem. Hoppe-Seyler, 372, 313-318; and (2) Nagatsu, T., Hino, M., Fuyamada, H., Hayakawa, T., Sakakibara, S., Nakagawa, Y., and Takemoto, T. (1976) Anal. Biochem., 74, 466-476, respectively. Human DPP-IV is also commercially available from, e.g., Research Diagnostics.
The activity of human DPP-IV was measured in vitro by its ability to cleave the synthetic substrate Gly-Pro-AMC. Cleavage of Gly-Pro-AMC by DPP-IV liberates the product AMC (7-amino-4-methyl coumarin), whose rate of appearance is directly proportional to the enzyme activity. Inhibition of the enzyme activity by specific enzyme inhibitors slows down the generation of AMC. Stronger interaction between an inhibitor and the enzyme results in a slower rate of generation of AMC. Thus, the degree of inhibition of the rate of accumulation of AMC is a direct measure of the strength of enzyme inhibition. The accumulation of AMC is measured fluorometrically. The IC50 (concentration of test compound at which 50% of the enzyme activity is inhibited) for each compound is determined by incubating fixed amounts of enzyme with several different concentrations of inhibitor. The inhibition constant, Ki, can be determined for each compound by incubating fixed amounts of enzyme with several different concentrations of inhibitor and substrate.
The compounds of the invention were tested for their ability to inhibit the activity of purified DPP-IV using the following methods. DPP-IV enzyme activity was determined by a fluorometric assay with the substrate Gly-Pro-AMC which is cleaved by DPP-IV to release the fluorescent AMC leaving group. Free AMC (7-amino-4-methyl coumarin) was measured using an excitation wavelength of 380 nm and an emission wavelength of 460 nm with a Victor-II fluorescent reader. Stock solutions of DPP-IV (1 ng/μl, pH 8.0) and Gly-Pro-AMC substrate (400 μM) in 25 mM Tris buffer (pH 8.0) were prepared separately. Test compounds were dissolved in DMSO or in 50 mM glycine buffer (pH 3.0). The assay was performed by diluting the DPP-IV stock (10 μl) into 25 mM Tris buffer (77.5 μl) followed by addition of test compound (2.5 μl) at 26° C. After 10-minutes substrate was added (10 μl) and allowed to react for 20-minutes at 26° C. before free AMC was measured. IC50 values were determined in triplicate, using a minimum of six different inhibitor concentrations. IC50 values were calculated using Nonlinear Regression Analysis (GraphPad, Prism, San Diego, Calif.).
Compounds 14 and 32 were tested in vitro as inhibitors of DPP-IV as described herein and each displayed an IC50 of 1 μM or less.
Example 4
DPP-VII, DPP-VIII, DPP-IX, and FAP Inhibitory Assays
Recombinant DPP-VII, DPP-VIII, DPP-IX, and FAP enzymes were prepared as follows. The full length cDNAs for human DPP-VII, DPP-VIII, DPP-IX, and FAP were obtained from Open Biosystems. The cDNAs were cloned into the pFastBac vector with the addition of an N-terminal FLAG tag on DPP-VII, C-terminal 6×His tags on DPP-VIII and DPP-IX, and an N-terminal 6×His tag on FAP (Sun 2002, Qi 2003, and Chen 2004). Baculovirus was prepared using the Bac-to-Bac Baculovirus Expression System (Invitrogen). The cDNAs in the final baculovirus constructs were sequence verified.
To express the recombinant DPPs from the baculovirus constructs, S9 insect cells (cells and Sf-900 SFM media purchased from Invitrogen) were grown to mid log phase at 27° C. with shaking at 125 rpm and then adjusted to 2×10E6/ml just prior to baculovirus infection. Infection with DPP-VII, DPP-VIII, DPP-IX, and FAP baculoviral constructs were all performed at an MOI of 4. The infected cells were grown for 48 hours and the cell pellets harvested and frozen until purification. DPP-VII was purified using anti-FLAG immunoaffinity gel according to the manufacturer's instructions. DPP-VIII, DPP-IX, and FAP were purified using a B-PER 6×His Fusion Protein Column Purification Kit from Pierce.
The activity of DPP-VII, DPP-VIII, DPP-IX and FAP was measured in vitro by its ability to cleave the synthetic substrates Lys-Pro AMC (DPP-VII) and Ala-Pro AMC (DPP-VIII, DPP-IX and FAP) (substrates purchased from Enzyme Systems). Recombinant DPPs were diluted in reaction buffer to give fluorescence values of 5000-20000 counts in the “enzyme only wells” 20 mm after addition of substrate at 27° C. Reaction buffers were 25 mM (2-(4-Morpholino)-Ethane Sulfonic Acid), pH 5.5 for DPP-VII, 25 mM Tris, pH 8, 1% Triton X-100, 100 mM NaCl for DPP-VIII, and 25 mM Tris pH 8 for DPP-IX and FAP. Test wells in a 96-well microtiter plate contained 88 μL of diluted DPP and 2.5 μl of titrated compound in 50 mM glycine, pH 2.6. “Enzyme only” wells contained 88 μL of diluted DPP and 2.5 μl of glycine buffer. “No enzyme” wells contained 88 μL of reaction buffer without DPP and 2.5 μl of glycine buffer. All assays were done in triplicate. The plate was incubated at 27° C. for 10 mm and then cooled on ice for 10 mm. Ten microliters of substrate diluted in reaction buffer (40 μM final concentration) without Triton or NaCl were then added to all wells followed by incubation at 27° C. for 20 mm. Fluorescence in each well was measured at settings of 380/460 nm. IC50 calculations were performed by non-linear regression analysis using Prism software (GraphPad).
Example 5
DPP Selectivity of Boronic Acids
Using the methods described above, the DPP-IV, VII, VIII, IX and FAP inhibitory activities of compounds 14 and 32, and the selectivity of compound 14 and 32 for DP-IV, were compared to the closely related analogues shown in Table 2.







TABLE 2







Other Boronic Acid Inhibitors








Com-



pound



Number
Structure





41






 


42






 


43






 


44






 


45






 


46






 


47






 


48













The selectivity ratio value was obtained by dividing the IC50 value for DPP-VII, DPP-VIII, DPP-IX or FAP by the IC50 value for DPP-IV and assigned a selectivity ratio defined as A≦1; 1<B≦10; 10<C≦50; D>50. Results are summarized in Table 3.







TABLE 3







DPP Selectivity Ratios








Compound
Selectivity Ratios*











No.
DPP-VII
DPP-VIII
DPP-IX
FAP





(+)−14
D
D
C
D


(−)−14
C
D
C
D


(+)−32
D
D
C
D


(−)−32
B
C
B
D


41
A
B
A
B


42
A
B
A
D


43
A
B
A
C


44
B
C
A
C


45
B
B
A
C


46
B
C
A
C


47
A
B
B
B


48
A
A
A
B





*Selectivity ratios: A ≦ 1; 1 < B ≦ 10; 10 < C ≦ 50; D > 50






Compounds (+)-14 and (+)-32, incorporating pyrrolidine containing substitutions at the 3-position of the ring, show greatly improved selectivity in contrast to their closely related analogues. For example, compound (+)-14 shows excellent selectivity for DPP-IV relative to DPP-VIII. Replacement of the 3-aminopyrrolidine with cyclopentyl 41, 4-piperidinyl 42, 3R-piperidinyl 43, or 3S-piperidinyl 45 (Table 2) maintains inhibition of DPP-IV. However, compounds 41, 43, 44, and 45 are significantly less selective towards the other dipeptidyl peptidases with one or more exhibiting selectivity ratios ≦1; and two or more exhibiting selectivity ratios ≦10. Thus, examples of the claimed compounds (+)-14 and (+)-32 demonstrate unexpected selectivity towards the other dipeptidyl peptidases while maintaining potency against DPP-IV, while the close structural analogues of the selective compounds of the invention exhibit significant activity against one or more of the other dipeptidyl peptidases.
Also, addition of further moieties at positions on the pyrrolidine ring produces compounds with reduced selectivity towards the other dipeptidyl peptidases. Alkylation of the pyrrolidine nitrogen yielding compounds 46 and 47, acylation of the pyrrolidine nitrogen yielding compound 48 or incorporation of a carboxamide yielding compound 49 all lead to a substantial loss of selectivity. See Table 2 for the structures of these compounds.
While the invention has been described and exemplified in sufficient detail for those skilled in this art to make and use it, various alternatives, modifications, and improvements will be apparent to those skilled in the art without departing from the spirit and scope of the claims.
All patents and publications are herein incorporated by reference to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference.
The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention that in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.

## Claims

1. A pyrrolidine compound represented by formula I:



a cyclic isomer thereof, any pharmaceutically acceptable salt thereof, or any prodrug thereof wherein,
R1 is methyl or ethyl;
R2 and R3 independently or together are —OH, —O−M+ wherein M+ is a cation, a hydroxyl bearing a boronic acid protecting group, or a group capable of being hydrolyzed to a hydroxyl group in an aqueous solution at physiological pH or in biological fluids; and
the wavy lines at asymmetric carbons Ca and Cb independently indicate for each asymmetric carbon an R configuration, an S configuration, or a mixture of both configurations such that all stereoisomers and all stereomeric mixtures are included.

2. A pyrrolidine compound of claim 1 having an R configuration at Ca.

3. The pyrrolidine compound of claim 2 having an R configuration at Cb.

4. The pyrrolidine compound of claim 2 having an S configuration at Cb.

5. An optically enriched stereoisomer of the pyrrolidine compound of claim 1.

6. A stereomeric mixture of two or more stereoisomers of the pyrrolidine compound of claim 1, wherein at least one of the asymmetric carbons, Ca or Cb, has both R and S configurations.

7. A stereomeric mixture of claim 6, which is a mixture of two diastereoisomers, an enantiomeric pair, a racemic mixture, a mixture of an enantiomeric pair plus a diastereomer, or an optically active mixture of at least two stereoisomers.

8. A stereomeric mixture of claim 6, wherein one stereoisomer predominates and the predominant isomer has an R configuration at Ca and an R or S configuration at Cb.

9. A stereomeric mixture of claim 8, wherein the predominant isomer has an R configuration at Cb.

10. A stereomeric mixture of claim 6, wherein two optical isomers predominate, one having R configurations at Ca and Cb, and one having an R configuration at Ca and an S configuration at Cb.

11. A pyrrolidine compound of claim 1 of the structure:




and having a stereomeric purity of at least about 55%.

12. A pyrrolidine compound of claim 11 having a stereomeric purity of at least about 90%.

13. A pyrrolidine compound of claim 11 having a stereomeric purity of at least about 98%.

14. A pyrrolidine compound of claim 1 having the structure:




and having a stereomeric purity of at least about 55%.

15. A pyrrolidine compound of claim 14 having a stereomeric purity of at least about 90%.

16. A pyrrolidine compound of claim 14 having a stereomeric purity of at least about 98%.

17. A pyrrolidine compound of claim 1 wherein the cyclic isomer form is represented by formula V:




wherein the asymmetric carbons Ca and Cb independently have an R configuration, an S configuration, or a mixture of both configurations such that all stereoisomers and all stereomeric mixtures are included.

18. A pharmaceutical combination comprising:
(a) a pyrrolidine compound represented by formula I:




a cyclic isomer thereof, any pharmaceutically acceptable salt thereof, or any prodrug thereof; wherein,
R1 is methyl or ethyl;
R2 and R3 independently or together are —OH, —O−M+ wherein M+ is a cation, a hydroxyl bearing a boronic acid protecting group, or a group capable of being hydrolyzed to a hydroxyl group in an aqueous solution at physiological pH or in biological fluids; and

the wavy lines at asymmetric carbons Ca and Cb independently indicate for each asymmetric carbon an R configuration, an S configuration, or a mixture of both configurations such that all stereoisomers and all stereomeric mixtures are included; and
(b) a second medicament comprising an antidiabetic agent that increases insulin secretion, increases insulin sensitivity, reduces the uptake of sugar from the gastrointestinal track, enhances the effect of endogenous peptides or proteins that affect glycemic control, provides a replacement for endogenous peptides or proteins that affect glycemic control, or any combination thereof.

19. A pharmaceutical combination of claim 18, wherein the second medicament is the antidiabetic agent.

20. A pharmaceutical combination of claim 19, wherein the weight ratio of the compound of formula I and the antidiabetic agent is from about 0.01:1 to about 100:1.

21. A pharmaceutical combination of claim 18, wherein the second medicament is glyburide, glipizide, nateglinide, repaglinide, metformin, pioglitazone, rosiglitazone, acarbose, miglitol, exenatide insulin, or any combination thereof.

22. A pharmaceutical combination of claim 18, wherein the second medicament is suitable for treatment of a diabetes-associated condition.

23. A pharmaceutical composition comprising
(a) a pyrrolidine compound represented by formula I:




a cyclic isomer thereof, any pharmaceutically acceptable salt thereof, or any prodrug thereof; wherein
R1 is methyl or ethyl;
R2 and R3 independently or together are —OH, —O−M+ wherein M+ is a cation, a hydroxyl bearing a boronic acid protecting group, or a group capable of being hydrolyzed to a hydroxyl group in an aqueous solution at physiological pH or in biological fluids; and

the wavy lines at asymmetric carbons Ca and Cb independently indicate for each asymmetric carbon an R configuration, an S configuration, or a mixture of both configurations such that all stereoisomers and stereomeric mixtures are included; and
(b) a pharmaceutically acceptable carrier.

24. A pharmaceutical composition of claim 23, further comprising (c) a second medicament selected from the group consisting of a known second medicament that increases insulin secretion, increases insulin sensitivity, reduces the uptake of sugar from the gastrointestinal track, enhances the effect of endogenous peptides or proteins that affect glycemic control, or provides a replacement for endogenous peptides, proteins that affect glycemic control, or any combination thereof.

25. A process for manufacture of a pharmaceutical composition of claim 23 comprising combining a pyrrolidine compound with a pharmaceutically acceptable carrier or diluent.

26. A process of claim 25, wherein the pharmaceutically acceptable carrier or diluent is suitable for oral administration or parenteral administration.

27. A process of claim 25, further comprising the step of formulating the composition into a tablet or capsule.

28. A process of claim 25, further comprising the step of lyophilizing the composition or the compound of formula I.

29. A pyrrolidine compound of claim 1, wherein R2 and R3 independently or together are the boronic acid protecting group formed from (+)-pinanediol; pinacol; 1,2-dicyclohexyl-ethanediol; 1,2-ethanediol; 2,2-diethanolamine; 1,3-propanediol; 2,3-butanediol, diisopropyl tartrate; 1,4-butanediol; diisopropylethanediol; (S,S,)-5,6-decanediol; 1,1,2-triphenyl-1,2-ethanediol; (2R,3R)-1,4-dimethoxy-1,1,4,4-tetraphenyl-2,3-butanediol; methanol; ethanol; isopropanol; catechol; or 1-butanol.

30. The pyrrolidine compound of claim 1, wherein R1 is methyl.

31. The pyrrolidine compound of claim 30, wherein R2 and R3 are both hydroxyl.