# Patent

## Title
Brassica oleracea plants with a resistance to Albugo candida

## Abstract

At least one embodiment of the present invention relates to Brassica oleracea plants with a resistance gene to Albugo candida, the cause of white blister. At least one embodiment of the invention also relates to a method for providing a Brassica oleracea plant with a resistance to Albugo candida, the at least one embodiment including a) providing a first B. oleracea plant which comprises a resistance gene to A. candida; (b) crossing the resistant plant with a susceptible second B. oleracea plant; (c) isolating genomic DNA from the progeny for detecting the presence of an introgression with the resistance gene using one or more specific DNA markers linked to the resistance gene; and (d) selecting from the progeny a B. oleracea plant in which the presence of the introgression with the resistance gene has been demonstrated in step (c).

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is the national phase under 35 U.S.C. §371 of PCT International Application No. PCT/NL2008/050232 which has an International filing date of 22 Apr. 2008, which designated the United States of America and which claims priority on Netherlands Application No. 2000622, the entire contents of each of which are hereby incorporated herein by reference.
REFERENCE TO A SEQUENCE LISTING
The material in the ASCII text file entitled 1470765—1.txt is hereby incorporated herein by reference in its entirety. The ASCII text file entitled 1470765—1.txt was created on 16 May 2012 and the size is 222 KB.
FIELD
The present invention relates to Brassica oleracea plants which are resistant to Albugo candida, the cause of white blister. The invention also relates to the seeds, fruits and/or other plant parts from these resistant plants. The present invention further relates to a method for providing B. oleracea plants which are resistant to A. candida. The invention also relates to the use of specific DNA markers which are linked to the A. candida resistance gene for the purpose of identifying resistant B. oleracea plants.
BACKGROUND
White blister (A. candida; synonyms: A. cruciferum, A. cruciferatum, white rust, staghead) is a plant disease which causes many problems in vegetables crops of cabbage, but also in related species such as rape, mustard and radish. The disease can in principle occur on all cruciferae, so also on wild species such as shepherd's purse (Capsella bursa-pastoris) and wild mustard (charlock mustard, Sinapis arvensis). Contrary to what the name suggests, this is not a rust fungus but an oomycete closely related to downy mildew (Peronospora parasitica) and Phytophtora. Oomycetes are not fungi and, although they also grow in threads, they are more related to algae.
The oomycete causes blisters with spores (sori, pustules) on the leaves, stems and ovaries (siliques) of Brassica plants. Distortions in the form of spots/are also often present. Systemic infection of plants results in abnormal growth, deformations and sometimes sterility of the flowers or inflorescence. The oomycete thrives best at temperatures between and 20° C. and in moist conditions. A leaf wetness period of 2.5 hours is sufficient to result in infection, at which there is an incubation period of 10 to 14 days. Moist weather conditions with moderate temperatures are therefore ideal for infection and spreading of the oomycete.
When spores of A. candida land on a cabbage leaf, they form a germ tube with which they penetrate the leaf. In here, the mycelium grows intercellularly and absorbs nutrients via haustoria. The vegetative spore formation takes place in the zoosporangium which develops under the epidermis. Created herein are the asexual zoospores which, when there is sufficient moisture, are released from the zoosporangia and can then cause new infections. The spores have two whiplash tails (flagellae), one for forward movement and one for the swimming direction.
A. candida can overwinter in the ground in sexual form with thick-walled oospores, which may or may not be on infected plant remnants, or in asexual form (mycelium) on winter-hardened host plants. During mild winters the oomycete does not really go to rest but remains active at a lower level. New plants can be infected in the spring. Plant material can also be already infected on the plant bed without symptoms becoming visible. Spread of the oomycete takes place through sporangia being carried away by air movements, hard rainfall, watering, machines, farm workers and insects, whereby other plants are infected.
Host specialization in A. candida is known and different physiological species and formae specialis are distinguished on the basis of the species or the line which is infected and the aggressiveness of the isolate on the line.
Brassica is a plant genus in the family Brassicaceae (formerly referred to as Cruciferae). The members of this genus are referred to as cabbage or mustard. The genus Brassica comprises a number of important agricultural and horticultural crops, including rape, cauliflower, red cabbage, savoy cabbage, white cabbage, oxheart cabbage, curly cale cabbage, broccoli, Brussels sprouts, Chinese cabbage, turnip cabbage and Portuguese cabbage (tronchuda). Almost all parts of the plants are used as food, such as the roots (turnip), stalks (turnip cabbage), leaves (white cabbage), axillary buds (sprouts), flowers (cauliflower, broccoli) and seeds (rape). Rape and rape seed are also used for oil, both for consumption and for fuel. Some species with white or purple flowers or distinct colour or shape of the leaves are cultivated for ornamental purposes. The Brassica family occurs worldwide and consists of annuals, biennials and perennials. The family also comprises a large number of wild species.
At the moment few agents are known which can be used to control white blister in Brassica. An increasing number of countries in Europe moreover have a policy aimed at reducing the use of crop protection agents. If the use of control agents is no longer allowed at all, this can result in major problems in the cultivation of Brassica species. In crops such as for instance Brassica rapa (syn. campestris) (turnip rape), Brassica juncea (mustard) and Brassica napus (rapeseed) white blister can cause huge losses in yield (Bernier, Can. Plant Dis. Surv. 52: 108, 1972; Fan et al., Can. J. Genet. Cytol. 25: 420-424, 1983); Harper and Pittman, Phytopathology 64: 408-410, 1974; Varshney et al., Theoretical and Applied Genetics 109: 153-159, 2004). In vegetable crops the quality aspect is particularly important. Vegetables such as sprouts, headed cabbage and curly cale cabbage infected by white blister are no longer sellable because of the cosmetic damage. There is therefore a great need for Brassica vegetable crops which are resistant to white blister.
Resistance to white blister is described in diverse Brassica species such as B. rapa, B. napus and B. juncea (Ebrahimi et al., Proc. Am. Phytopathol. Soc. 3: 273, 1976; Delwiche and Williams, Proc. Am. Phytopathol. Soc. 1: 66, 1974; Tiwari et al., Can. J. Of Plant Science 68: 297-300, 1988; Kole et al., Genome 45: 22-27, 2002; Varshney et al., Theoretical and Applied Genetics 109: 153-159, 2004; Tanhuanpad, Theoretical and Applied Genetics 108: 1039-1046, 2004). In addition, partial resistance has been demonstrated in B. oleracea lines (Santos and Dias, Genetic Resources and Crop Evolution 51: 713-722, 2004). Full resistance to white blister in B. oleracea vegetable crops has however not as yet been described.
SUMMARY
An object of the present invention is to provide a B. oleracea plant with a resistance to A. candida, the cause of white blister.
The invention provides to this end a B. oleracea plant comprising a resistance gene to A. candida. 


DETAILED DESCRIPTION
The resistance gene according to the invention provides a monogenic and dominant resistance to A. candida. The resistance gene is preferably present in heterozygous form, and more preferably the resistance gene is present in homozygous form.
According to the invention the resistance gene to A. candida preferably comes from the B. oleracea plant, the seeds of which were deposited in the American Type Culture Collection (ATCC, Patent Depository, 10801 University Boulevard, Manassas, Va. 20110, United States of America) on 1 Mar. 2006 under number PTA 74-12. Surprisingly, it has been found that with the resistance gene according to the invention a dominant resistance is provided to A. candida. 
In order to obtain a full resistance to A. candida in B. oleracea the transmission is described in this invention of a dominant, monogenic resistance to A. candida from a first B. oleracea source to different other B. oleracea types such as white cabbage, Brussels sprouts, cauliflower and turnip cabbage.
Using a disease test for white blister resistance B. oleracea lines were screened and a white blister resistance source was identified. The resistance was then transmitted from the source to existing quality lines by means of repeated backcrossing, in some cases as many as four to six times, followed by multiple generations of self-pollination. A disease test was performed here each time in order to select the resistant plants for the continuation of the backcrossing program. In the evaluation of these disease tests, plants were grouped into the classes resistant (no visible reaction or necrotic spots), susceptible (many sporulating blisters) and intermediate (necrotic spots and several sporulating blisters). It was found from the segregation ratios found during the backcrossing program that the resistance was a monogenic dominant trait. A lack of resistant plants was however found in many genetic backgrounds and, in addition, a great variation in numbers in the intermediate class (from several plants to half the population). The penetration of this gene was thus very incomplete in these genetic backgrounds and the breeding program was greatly hampered as a result.
In a further preferred embodiment of the invention the resistance gene is linked to one or more specific DNA markers. So as to be better able to monitor the resistance and transmit it more quickly, DNA markers have been developed according to the present invention which are closely linked to the introgression having thereon the disease-resistance gene against white blister. These markers have been developed by means of a BSA (Bulked Segregant Analysis). For this purpose individuals from a correct (1:1) segregating BC population were divided on the basis of the disease test into a resistant and a susceptible class. DNA was then isolated from all plants, and the resistant plants were bulked to form a resistant pool, and the susceptible plants to form a susceptible pool. Marker analyses were then performed on these pools by means of the RAMP technique and markers were identified which were closely linked to the resistance. By means of an analysis with the closely linked markers the plants were selected with certainty which contained the resistance gene in populations where the disease test does not give an unambiguous picture (many intermediary reactions, not a good segregation ratio). In addition, the homozygous resistant plants are directly differentiated from the heterozygous resistant plants during inbreeding. This results in an accelerated breeding program.
In a preferred embodiment of the invention the presence of the introgression with the resistance gene to A. candida can be demonstrated using at least two, preferably at least three, more preferably at least four, more preferably at least five, six, seven or eight, most preferably nine DNA markers linked to the resistance gene, wherein the DNA markers enclose the resistance gene. Enclose in the present application is understood to mean that the DNA markers are located on the genome on both sides of the resistance gene, i.e. “upstream” as well as “downstream” of the resistance gene. Demonstrating the presence of a plurality of DNA markers, which are linked to the resistance gene, and moreover enclosing the resistance gene ensure that the introgression with the resistance gene is actually present.
The DNA markers according to the invention are preferably chosen from table 1, wherein the presence of the DNA markers in the genome of the plant is demonstrated using the primer sequences chosen from the group consisting of SEQ ID NO: 1 up to and including SEQ ID NO: 10 (table 2).
In the research which has led to the present invention it has been demonstrated that the relevant DNA markers are characteristic for the introgression of the resistance to A. candida. The DNA markers according to the invention are DNA fragments which are linked to the relevant resistance gene, have a determined size (bp) as indicated in table 1, and can be demonstrated by using specific primer combinations.
The plant according to the invention is preferably chosen from the group consisting of B. oleracea convar. botrytis var. botrytis (cauliflower, romanesco), B. oleracea convar. botrytis var. cymosa (broccoli), B. oleracea convar. botrytis var. asparagoides (sprouting broccoli), B. oleracea convar. oleracea var. gemnifera (Brussels sprouts), B. oleracea convar. capitata var. alba (white cabbage, oxheart cabbage), B. oleracea convar. capitata var. rubra (red cabbage), B. oleracea convar. capitata var. sabauda (savoy cabbage), B. oleracea convar. acephela var. sabellica (curly cale cabbage), B. oleracea convar. acephela var. gongyloides (turnip cabbage) and B. oleracea var. tronchuda syn. costata (Portuguese cabbage).
The invention also relates to the seeds, fruits and/or other plant parts from the above described plants. Plant parts are here understood to mean, among others, the edible parts of the plant, such as for instance axillary buds (sprouts).
The invention also relates to a method for obtaining a B. oleracea plant with a resistance to A. candida, which method comprises at least the following steps of:
(a) providing a first B. oleracea plant, which plant comprises a resistance gene to A. candida; 
(b) crossing the resistant plant with a susceptible second B. oleracea plant;
(c) isolating genomic DNA from the progeny for detecting the presence of an introgression with the resistance gene using one or more specific DNA markers linked to the resistance gene; and
(d) selecting from the progeny a B. oleracea plant in which the presence of the introgression with the resistance gene has been demonstrated in step (c).
With the method according to the invention resistant B. oleracea plants can be provided in rapid and simple manner by making use of DNA markers which are specific to the introgression with the resistance gene according to the invention.
Using the method according to the present invention and the use of the specific DNA markers linked to a resistance gene it is possible to determine in simple manner wether a plant contains the resistance gene. Performing the disease test is a very time-consuming procedure. Selection of resistant plants by utilizing the specific DNA markers linked to a resistance gene is much more efficient. Larger numbers of plants can hereby be tested more easily. The introgression with the resistance gene can also be more readily mapped, whereby plants with the smallest possible introgression can be selected. Furthermore, distinction can be made between homozygous and heterozygous resistant plants.
The plants selected in step (d) of the method according to the invention can optionally be subjected to additional steps, such as back-crossing or self-pollination of the plant obtained in step (d) one or more times with a susceptible B. oleracea plant and subsequently selecting once again from the progeny a resistant B. oleracea plant using the specific DNA markers. The plants obtained in step (d) can for instance also be made homozygous by means of techniques known to the skilled person such as anther and/or microspore culture.
In a preferred embodiment of the method the presence of the introgression with the resistance gene in the selected plants is confirmed by means of a disease test. The presence and effect of the resistance gene can be definitively confirmed by performing a disease test.
The first B. oleracea plant preferably comprises a resistance gene which gives a monogenic and dominant resistance to A. candida. In a preferred embodiment of the invention the resistance gene is present in heterozygous form, preferably in a homozygous form.
In a preferred embodiment the first B. oleracea plant comprises a resistance gene from the B. oleracea plant, the seeds of which were deposited in the American Type Culture Collection (ATCC, Patent Depository, 10801 University Boulevard, Manassas, Va. 20110, United States of America) on 1 Mar. 2006 under number PTA 74-12.
In a further preferred embodiment of the method according to the invention the selection of the resistant B. oleracea plant in step (d) comprises of selecting a B. oleracea plant which comprises at least two, preferably at least three, more preferably at least four, more preferably at least five, six, seven or eight, and most preferably nine DNA markers linked to the resistance gene, wherein the DNA markers enclose the resistance gene. It is hereby possible to determine with certainty that the plant actually possesses the introgression with the resistance gene.
The DNA markers according to the invention are preferably chosen from table 1, wherein the presence of the DNA markers in the genome of the plant is demonstrated using the primer sequences chosen from the group consisting of SEQ ID NO: 1 up to and including SEQ ID NO: 10 (table 2).
In a particular embodiment according to the invention the first B. oleracea plant comprises a resistance gene to A. candida originating from the B. oleracea plant, the seeds of which were deposited in the American Type Culture Collection (ATCC, Patent Depository, 10801 University Boulevard, Manassas, Va. 20110, United States of America) on 1 Mar. 2006 under number PTA 74-12.)
The susceptible B. oleracea plant into which the resistance gene is inserted is preferably chosen from the group consisting of B. oleracea convar. botrytis var. botrytis (cauliflower, romanesco), B. oleracea convar. botrytis var. cymosa (broccoli), B. oleracea convar. botrytis var. asparagoides (sprouting broccoli), B. oleracea convar. oleracea var. gemnifera (Brussels sprouts), B. oleracea convar. capitata var. alba (white cabbage, oxheart cabbage), B. oleracea convar. capitata var. rubra (red cabbage), B. oleracea convar. capitata var. sabauda (savoy cabbage) B. oleracea convar. acephela var. sabellica (curly cale cabbage), B. oleracea convar. acephela var. gongyloides (turnip cabbage) and B. oleracea var. tronchuda syn. costata (Portuguese cabbage).
The invention further relates to the B. oleracea plants obtainable with the above described method, and to the seeds and/or plant parts thereof.
The invention also relates to the use of at least one DNA marker linked to a resistance gene to A. candida for identifying a B. oleracea plant which is resistant to A. candida, wherein the DNA marker is chosen from the DNA markers of table 1 and wherein the DNA marker is demonstrated with the primer sequences chosen from the group consisting of SEQ ID NO: 1 up to and including SEQ ID NO: 10 (table 2).
The resistance gene preferably originates from the B. oleracea plant of which the seeds were deposited in the American Type Culture Collection (ATCC, Patent Depository, 10801 University Boulevard, Manassas, Va. 20110, United States of America) on 1 Mar. 2006 under number PTA 74-12.
EXAMPLES
The invention is further elucidated on the basis of the following examples.
Example 1
Populations and Disease Test
The white blister resistance source originates from the parent line 9002757 of Bejo Zaden BV, seeds of which were deposited at the (ATCC, Patent Depository, 10801 University Boulevard, Manassas, Va. 20110, United States of America) on 1 Mar. 2006 under number PTA 74-12. Using this source crossings were made with different B. oleracea species (curly cale cabbage, turnip cabbage, broccoli, sprouting broccoli, white cabbage, oxheart cabbage, red cabbage, savoy cabbage, tronchuda, Brussels sprouts and cauliflower). BC1 populations were obtained after backcrossing with susceptible parent lines. Use was made of a disease test in order to select the resistant plants from these populations.
In order to preserve A. candida isolates which are used for the disease test, zoosporangia from susceptible B. oleracea plants from the field were isolated. After germination in water the spores were used to inoculate susceptible plants. After development of the blisters, these zoosporangia were harvested and stored in liquid nitrogen until use. The eventual disease test took place in the glasshouse on seedlings of the BC1 population, the seed leaves of which had developed 24 to 48 hours before. The plants were inoculated with a fresh zoospore suspension (5×104 zoospores per ml) which was prepared by washing zoosporangia from susceptible plants and allowing them to germinate in water. Several drops of zoospore suspension were pipetted onto the seed leaves. After the pipetting the plants were further grown under a plastic tunnel in order to ensure optimum conditions for infection. Two weeks after inoculation the plants were assessed, wherein they were grouped into the classes resistant, susceptible or intermediate (Williams, Screening crucifers for multiple disease resistance. Workshop, Sep. 2-3, 1981, J.F. Friedrick Center, University of Wisconsin, Madison, USA).
After performing of the disease test on the seedlings, the resistant plants were retained for the following step in the backcrossing program. The results of the disease test showed that the resistance was in principle a monogenic dominant trait. Plants with intermediate reactions were however also often found in addition to plants with susceptible and resistant reactions. This was found to be highly dependent upon the genetic background in which work was being done. Different populations were selected from the programme in which there was no, or hardly any, intermediate reaction and in which the expected segregation ratio (1:1 for a BC and 3:1 for a self-pollination) was also found.
Example 2
Marker Development
For the development of linked DNA markers, four populations of about 200 individuals were used (cauliflower, curly cale cabbage, tronchuda, white cabbage). DNA of all individuals was isolated from leaf punches ( ̃0.3 cm2/leaf punch). A BSA method was used to generate closely linked DNA markers, with the aid of the RAMP technique (Random Amplified Microsatellite Polymorphisms) (Matsumoto et al., Mammalian Genome 9: 531-535, 1998; Reiter, PCR-based marker systems, in: R. L. Philips & I. K. Vasil (eds.), DNA-based markers in plants, Kluwer Academic Publishers, 2001; Weising et al., DNA fingerprinting in plants, principles, methods and application, CRC Press, 2nd ed., 21-73, 2005).
The RAMP technique, wherein an iSSR and a RAPD-primer were combined, produced band patterns having fragments therein which specifically co-segregated with the resistance, and wherein a distinction could be made between plants with and without the resistance gene. By mapping the RAMP-fragments, closely linked RAMP-markers were identified which enclose the resistance gene.
Example 3
PCR Conditions and Marker Analysis
The PCR conditions used for the RAMP reactions are as follows:
PCR mix
75 mM Tris-HCL (pH 8.8)
20 mM NH4SO4
0.01% (v/v) Tween20
2.8 mM MgCl2
0.25 mM dNTPs
0.15 μM forward primer
0.2 μM reverse primer
0.04 units/μl Red Hot® DNA Polymerase (ABgene, Epsom, UK)
 ̃0.2 ng/μl genomic plant DNA
PCR Program:
step 1: 2 min. 93° C.
step 2: 30 sec. 93° C.
step 3: 30 sec. 35° C.
step 4: heating by 0.3°/sec to 72° C.
step 5: 1 min. 30 sec 72° C.
steps 2-5: repeat 40×
step 6: 5 min 72° C.
Polyacryl Gel Electrophoresis
For analysis of the RAMP patterns use was made of “Gene ReadIR 4200 DNA analyzers” (Licor Inc.). On the basis of an optimal concentration of 6.5% acryl amide, fragments can be separated which have a difference in size of a single base.
In order to make the fragments visible on this system it is necessary to use labelled (IRDye labels) primers. For this purpose a third of the quantity of the forward primer was replaced by a labelled primer with the same sequence.
Example 4
Marker Overview
Given in tables 1 and 2 for the different RAMP markers are the sequences of the primers, the size of the informative fragment and the estimated distance from the resistance in cM based on the number of crossing-overs in the population. Analysis of the number of crossing-overs between the different markers shows that the markers enclose the resistance gene.







TABLE 1







Overview of the RAMP markers









RAMP SEQ ID
Fragment
Position in cM relative


Combination
size (bp)
to resistance gene












1 + 10-1209
325
+6.1


2 + 10-1209
393
+4.6


3 + 10-1209
508
+4.2


4 + 10-1209
830
−1.2


5 + 10-1209
285
−2.0


6 + 10-1209
607
−8.8


7 + 10-1209
875
+0.1


8 + 10-1209
291
0.0


9 + 10-1209
138
−0.1





where + and − indicate that the markers lie on either side of the disease-resistance gene













TABLE 2







Overview of SEQ ID nos.








SEQ ID



no.
Sequence





1 iSSR
CAGGAAACAGCTATGACAAAAAGAGAGAGAGAG


 


2 iSSR
CAGGAAACAGCTATGACTACGACACACACACAC


 


3 iSSR
CAGGAAACAGCTATGACATACATATATATATATATAT


 


4 iSSR
CAGGAAACAGCTATGACCCAGGTGTGTGTGTGT


 


5 iSSR
CAGGAAACAGCTATGACAGTGGAGAGAGAGAGAG


 


6 iSSR
CAGGAAACAGCTATGACACTATCTCTCTCTCTC


 


7 iSSR
CAGGAAACAGCTATGACATCTTCATCATCATCA


 


8 iSSR
CAGGAAACAGCTATGACGTTTGAGAGAGAGA


 


9 iSSR
CAGGAAACAGCTATGACCCCACAACAACAACAA





10 Operon RAPD ® 10-mer kits A-01 to BH-20













TABLE 3







Operon RAPD ® 10-mer kits A-01 to BH-20












Kit
Primer

SEQ ID



Name
Name
Sequence
NO:















KIT A
OPA-01
CAGGCCCTTC
10


 




OPA-02
TGCCGAGCTG
11


 




OPA-03
AGTCAGCCAC
12


 




OPA-04
AATCGGGCTG
13


 




OPA-05
AGGGGTCTTG
14


 




OPA-06
GGTCCCTGAC
15


 




OPA-07
GAAACGGGTG
16


 




OPA-08
GTGACGTAGG
17


 




OPA-09
GGGTAACGCC
18


 




OPA-10
GTGATCGCAG
19


 




OPA-11
CAATCGCCGT
20


 




OPA-12
TCGGCGATAG
21


 




OPA-13
CAGCACCCAC
22


 




OPA-14
TCTGTGCTGG
23


 




OPA-15
TTCCGAACCC
24


 




OPA-16
AGCCAGCGAA
25


 




OPA-17
GACCGCTTGT
26


 




OPA-18
AGGTGACCGT
27


 




OPA-19
CAAACGTCGG
28


 




OPA-20
GTTGCGATCC
29


 



KIT B
OPB-01
GTTTCGCTCC
30


 




OPB-02
TGATCCCTGG
31


 




OPB-03
CATCCCCCTG
32


 




OPB-04
GGACTGGAGT
33


 




OPB-05
TGCGCCCTTC
34


 




OPB-06
TGCTCTGCCC
35


 




OPB-07
GGTGACGCAG
36


 




OPB-08
GTCCACACGG
37


 




OPB-09
TGGGGGACTC
38


 




OPB-10
CTGCTGGGAC
39


 




OPB-11
GTAGACCCGT
40


 




OPB-12
CCTTGACGCA
41


 




OPB-13
TTCCCCCGCT
42


 




OPB-14
TCCGCTCTGG
43


 




OPB-15
GGAGGGTGTT
44


 




OPB-16
TTTGCCCGGA
45


 




OPB-17
AGGGAACGAG
46


 




OPB-18
CCACAGCAGT
47


 




OPB-19
ACCCCCGAAG
48


 




OPB-20
GGACCCTTAC
49


 



KIT C
OPC-01
TTCGAGCCAG
50


 




OPC-02
GTGAGGCGTC
51


 




OPC-03
GGGGGTCTTT
52


 




OPC-04
CCGCATCTAC
53


 




OPC-05
GATGACCGCC
54


 




OPC-06
GAACGGACTC
55


 




OPC-07
GTCCCGACGA
56


 




OPC-08
TGGACCGGTG
57


 




OPC-09
CTCACCGTCC
58


 




OPC-10
TGTCTGGGTG
59


 




OPC-11
AAAGCTGCGG
60


 




OPC-12
TGTCATCCCC
61


 




OPC-13
AAGCCTCGTC
62


 




OPC-14
TGCGTGCTTG
63


 




OPC-15
GACGGATCAG
64


 




OPC-16
CACACTCCAG
65


 




OPC-17
TTCCCCCCAG
66


 




OPC-18
TGAGTGGGTG
67


 




OPC-19
GTTGCCAGCC
68


 




OPC-20
ACTTCGCCAC
69


 



KIT D
OPD-01
ACCGCGAAGG
70


 




OPD-02
GGACCCAACC
71


 




OPD-03
GTCGCCGTCA
72


 




OPD-04
TCTGGTGAGG
73


 




OPD-05
TGAGCGGACA
74


 




OPD-06
ACCTGAACGG
75


 




OPD-07
TTGGCACGGG
76


 




OPD-08
GTGTGCCCCA
77


 




OPD-09
CTCTGGAGAC
78


 




OPD-10
GGTCTACACC
79


 




OPD-11
AGCGCCATTG
80


 




OPD-12
CACCGTATCC
81


 




OPD-13
GGGGTGACGA
82


 




OPD-14
CTTCCCCAAG
83


 




OPD-15
CATCCGTGCT
84


 




OPD-16
AGGGCGTAAG
85


 




OPD-17
TTTCCCACGG
86


 




OPD-18
GAGAGCCAAC
87


 




OPD-19
CTGGGGACTT
88


 




OPD-20
ACCCGGTCAC
89


 



KIT E
OPE-01
CCCAAGGTCC
90


 




OPE-02
GGTGCGGGAA
91


 




OPE-03
CCAGATGCAC
92


 




OPE-04
GTGACATGCC
93


 




OPE-05
TCAGGGAGGT
94


 




OPE-06
AAGACCCCTC
95


 




OPE-07
AGATGCAGCC
96


 




OPE-08
TCACCACGGT
97


 




OPE-09
CTTCACCCGA
98


 




OPE-10
CACCAGGTGA
99


 




OPE-11
GAGTCTCAGG
100


 




OPE-12
TTATCGCCCC
101


 




OPE-13
CCCGATTCGG
102


 




OPE-14
TGCGGCTGAG
103


 




OPE-15
ACGCACAACC
104


 




OPE-16
GGTGACTGTG
105


 




OPE-17
CTACTGCCGT
106


 




OPE-18
GGACTGCAGA
107


 




OPE-19
ACGGCGTATG
108


 




OPE-20
AACGGTGACC
109


 



KIT F
OPF-01
ACGGATCCTG
110


 




OPF-02
GAGGATCCCT
111


 




OPF-03
CCTGATCACC
112


 




OPF-04
GGTGATCAGG
113


 




OPF-05
CCGAATTCCC
114


 




OPF-06
GGGAATTCGG
115


 




OPF-07
CCGATATCCC
116


 




OPF-08
GGGATATCGG
117


 




OPF-09
CCAAGCTTCC
118


 




OPF-10
GGAAGCTTGG
119


 




OPF-11
TTGGTACCCC
120


 




OPF-12
ACGGTACCAG
121


 




OPF-13
GGCTGCAGAA
122


 




OPF-14
TGCTGCAGGT
123


 




OPF-15
CCAGTACTCC
124


 




OPF-16
GGAGTACTGG
125


 




OPF-17
AACCCGGGAA
126


 




OPF-18
TTCCCGGGTT
127


 




OPF-19
CCTCTAGACC
128


 




OPF-20
GGTCTAGAGG
129


 



KIT G
OPG-01
CTACGGAGGA
130


 




OPG-02
GGCACTGAGG
131


 




OPG-03
GAGCCCTCCA
132


 




OPG-04
AGCGTGTCTG
133


 




OPG-05
CTGAGACGGA
134


 




OPG-06
GTGCCTAACC
135


 




OPG-07
GAACCTGCGG
136


 




OPG-08
TCACGTCCAC
137


 




OPG-09
CTGACGTCAC
138


 




OPG-10
AGGGCCGTCT
139


 




OPG-11
TGCCCGTCGT
140


 




OPG-12
CAGCTCACGA
141


 




OPG-13
CTCTCCGCCA
142


 




OPG-14
GGATGAGACC
143


 




OPG-15
ACTGGGACTC
144


 




OPG-16
AGCGTCCTCC
145


 




OPG-17
ACGACCGACA
146


 




OPG-18
GGCTCATGTG
147


 




OPG-19
GTCAGGGCAA
148


 




OPG-20
TCTCCCTCAG
149


 



KIT H
OPH-01
GGTCGGAGAA
150


 




OPH-02
TCGGACGTGA
151


 




OPH-03
AGACGTCCAC
152


 




OPH-04
GGAAGTCGCC
153


 




OPH-05
AGTCGTCCCC
154


 




OPH-06
ACGCATCGCA
155


 




OPH-07
CTGCATCGTG
156


 




OPH-08
GAAACACCCC
157


 




OPH-09
TGTAGCTGGG
158


 




OPH-10
CCTACGTCAG
159


 




OPH-11
CTTCCGCAGT
160


 




OPH-12
ACGCGCATGT
161


 




OPH-13
GACGCCACAC
162


 




OPH-14
ACCAGGTTGG
163


 




OPH-15
AATGGCGCAG
164


 




OPH-16
TCTCAGCTGG
165


 




OPH-17
CACTCTCCTC
166


 




OPH-18
GAATCGGCCA
167


 




OPH-19
CTGACCAGCC
168


 




OPH-20
GGGAGACATC
169


 



KIT I
OPI-01
ACCTGGACAC
170


 




OPI-02
GGAGGAGAGG
171


 




OPI-03
CAGAAGCCCA
172


 




OPI-04
CCGCCTAGTC
173


 




OPI-05
TGTTCCACGG
174


 




OPI-06
AAGGCGGCAG
175


 




OPI-07
CAGCGACAAG
176


 




OPI-08
TTTGCCCGGT
177


 




OPI-09
TGGAGAGCAG
178


 




OPI-10
ACAACGCGAG
179


 




OPI-11
ACATGCCGTG
180


 




OPI-12
AGAGGGCACA
181


 




OPI-13
CTGGGGCTGA
182


 




OPI-14
TGACGGCGGT
183


 




OPI-15
TCATCCGAGG
184


 




OPI-16
TCTCCGCCCT
185


 




OPI-17
GGTGGTGATG
186


 




OPI-18
TGCCCAGCCT
187


 




OPI-19
AATGCGGGAG
188


 




OPI-20
AAAGTGCGGG
189


 



KIT J
OPJ-01
CCCGGCATAA
190


 




OPJ-02
CCCGTTGGGA
191


 




OPJ-03
TCTCCGCTTG
192


 




OPJ-04
CCGAACACGG
193


 




OPJ-05
CTCCATGGGG
194


 




OPJ-06
TCGTTCCGCA
195


 




OPJ-07
CCTCTCGACA
196


 




OPJ-08
CATACCGTGG
197


 




OPJ-09
TGAGCCTCAC
198


 




OPJ-10
AAGCCCGAGG
199


 




OPJ-11
ACTCCTGCGA
200


 




OPJ-12
GTCCCGTGGT
201


 




OPJ-13
CCACACTACC
202


 




OPJ-14
CACCCGGATG
203


 




OPJ-15
TGTAGCAGGG
204


 




OPJ-16
CTGCTTAGGG
205


 




OPJ-17
ACGCCAGTTC
206


 




OPJ-18
TGGTCGCAGA
207


 




OPJ-19
GGACACCACT
208


 




OPJ-20
AAGCGGCCTC
209


 



KIT K
OPK-01
CATTCGAGCC
210


 




OPK-02
GTCTCCGCAA
211


 




OPK-03
CCAGCTTAGG
212


 




OPK-04
CCGCCCAAAC
213


 




OPK-05
TCTGTCGAGG
214


 




OPK-06
CACCTTTCCC
215


 




OPK-07
AGCGAGCAAG
216


 




OPK-08
GAACACTGGG
217


 




OPK-09
CCCTACCGAC
218


 




OPK-10
GTGCAACGTG
219


 




OPK-11
AATGCCCCAG
220


 




OPK-12
TGGCCCTCAC
221


 




OPK-13
GGTTGTACCC
222


 




OPK-14
CCCGCTACAC
223


 




OPK-15
CTCCTGCCAA
224


 




OPK-16
GAGCGTCGAA
225


 




OPK-17
CCCAGCTGTG
226


 




OPK-18
CCTAGTCGAG
227


 




OPK-19
CACAGGCGGA
228


 




OPK-20
GTGTCGCGAG
229


 



KIT L
OPL-01
GGCATGACCT
230


 




OPL-02
TGGGCGTCAA
231


 




OPL-03
CCAGCAGCTT
232


 




OPL-04
GACTGCACAC
233


 




OPL-05
ACGCAGGCAC
234


 




OPL-06
GAGGGAAGAG
235


 




OPL-07
AGGCGGGAAC
236


 




OPL-08
AGCAGGTGGA
237


 




OPL-09
TGCGAGAGTC
238


 




OPL-10
TGGGAGATGG
239


 




OPL-11
ACGATGAGCC
240


 




OPL-12
GGGCGGTACT
241


 




OPL-13
ACCGCCTGCT
242


 




OPL-14
GTGACAGGCT
243


 




OPL-15
AAGAGAGGGG
244


 




OPL-16
AGGTTGCAGG
245


 




OPL-17
AGCCTGAGCC
246


 




OPL-18
ACCACCCACC
247


 




OPL-19
GAGTGGTGAC
248


 




OPL-20
TGGTGGACCA
249


 



KIT M
OPM-01
GTTGGTGGCT
250


 




OPM-02
ACAACGCCTC
251


 




OPM-03
GGGGGATGAG
252


 




OPM-04
GGCGGTTGTC
253


 




OPM-05
GGGAACGTGT
254


 




OPM-06
CTGGGCAACT
255


 




OPM-07
CCGTGACTCA
256


 




OPM-08
TCTGTTCCCC
257


 




OPM-09
GTCTTGCGGA
258


 




OPM-10
TCTGGCGCAC
259


 




OPM-11
GTCCACTGTG
260


 




OPM-12
GGGACGTTGG
261


 




OPM-13
GGTGGTCAAG
262


 




OPM-14
AGGGTCGTTC
263


 




OPM-15
GACCTACCAC
264


 




OPM-16
GTAACCAGCC
265


 




OPM-17
TCAGTCCGGG
266


 




OPM-18
CACCATCCGT
267


 




OPM-19
CCTTCAGGCA
268


 




OPM-20
AGGTCTTGGG
269


 



KIT N
OPN-01
CTCACGTTGG
270


 




OPN-02
ACCAGGGGCA
271


 




OPN-03
GGTACTCCCC
272


 




OPN-04
GACCGACCCA
273


 




OPN-05
ACTGAACGCC
274


 




OPN-06
GAGACGCACA
275


 




OPN-07
CAGCCCAGAG
276


 




OPN-08
ACCTCAGCTC
277


 




OPN-09
TGCCGGCTTG
278


 




OPN-10
ACAACTGGGG
279


 




OPN-11
TCGCCGCAAA
280


 




OPN-12
CACAGACACC
281


 




OPN-13
AGCGTCACTC
282


 




OPN-14
TCGTGCGGGT
283


 




OPN-15
CAGCGACTGT
284


 




OPN-16
AAGCGACCTG
285


 




OPN-17
CATTGGGGAG
286


 




OPN-18
GGTGAGGTCA
287


 




OPN-19
GTCCGTACTG
288


 




OPN-20
GGTGCTCCGT
289


 



KIT O
OPO-01
GGCACGTAAG
290


 




OPO-02
ACGTAGCGTC
291


 




OPO-03
CTGTTGCTAC
292


 




OPO-04
AAGTCCGCTC
293


 




OPO-05
CCCAGTCACT
294


 




OPO-06
CCACGGGAAG
295


 




OPO-07
CAGCACTGAC
296


 




OPO-08
CCTCCAGTGT
297


 




OPO-09
TCCCACGCAA
298


 




OPO-10
TCAGAGCGCC
299


 




OPO-11
GACAGGAGGT
300


 




OPO-12
CAGTGCTGTG
301


 




OPO-13
GTCAGAGTCC
302


 




OPO-14
AGCATGGCTC
303


 




OPO-15
TGGCGTCCTT
304


 




OPO-16
TCGGCGGTTC
305


 




OPO-17
GGCTTATGCC
306


 




OPO-18
CTCGCTATCC
307


 




OPO-19
GGTGCACGTT
308


 




OPO-20
ACACACGCTG
309


 



KIT P
OPP-01
GTAGCACTCC
310


 




OPP-02
TCGGCACGCA
311


 




OPP-03
CTGATACGCC
312


 




OPP-04
GTGTCTCAGG
313


 




OPP-05
CCCCGGTAAC
314


 




OPP-06
GTGGGCTGAC
315


 




OPP-07
GTCCATGCCA
316


 




OPP-08
ACATCGCCCA
317


 




OPP-09
GTGGTCCGCA
318


 




OPP-10
TCCCGCCTAC
319


 




OPP-11
AACGCGTCGG
320


 




OPP-12
AAGGGCGAGT
321


 




OPP-13
GGAGTGCCTC
322


 




OPP-14
CCAGCCGAAC
323


 




OPP-15
GGAAGCCAAC
324


 




OPP-16
CCAAGCTGCC
325


 




OPP-17
TGACCCGCCT
326


 




OPP-18
GGCTTGGCCT
327


 




OPP-19
GGGAAGGACA
328


 




OPP-20
GACCCTAGTC
329


 



KIT Q
OPQ-01
GGGACGATGG
330


 




OPQ-02
TCTGTCGGTC
331


 




OPQ-03
GGTCACCTCA
332


 




OPQ-04
AGTGCGCTGA
333


 




OPQ-05
CCGCGTCTTG
334


 




OPQ-06
GAGCGCCTTG
335


 




OPQ-07
CCCCGATGGT
336


 




OPQ-08
CTCCAGCGGA
337


 




OPQ-09
GGCTAACCGA
338


 




OPQ-10
TGTGCCCGAA
339


 




OPQ-11
TCTCCGCAAC
340


 




OPQ-12
AGTAGGGCAC 
341


 




OPQ-13
GGAGTGGACA
342


 




OPQ-14
GGACGCTTCA
343


 




OPQ-15
GGGTAACGTG
344


 




OPQ-16
AGTGCAGCCA
345


 




OPQ-17
GAAGCCCTTG
346


 




OPQ-18
AGGCTGGGTG
347


 




OPQ-19
CCCCCTATCA
348


 




OPQ-20
TCGCCCAGTC
349


 



KIT R
OPR-01
TGCGGGTCCT
350


 




OPR-02
CACAGCTGCC
351


 




OPR-03
ACACAGAGGG
352


 




OPR-04
CCCGTAGCAC
353


 




OPR-05
GACCTAGTGG
354


 




OPR-06
GTCTACGGCA
355


 




OPR-07
ACTGGCCTGA
356


 




OPR-08
CCCGTTGCCT
357


 




OPR-09
TGAGCACGAG
358


 




OPR-10
CCATTCCCCA
359


 




OPR-11
GTAGCCGTCT
360


 




OPR-12
ACAGGTGCGT
361


 




OPR-13
GGACGACAAG
362


 




OPR-14
CAGGATTCCC
363


 




OPR-15
GGACAACGAG
364


 




OPR-16
CTCTGCGCGT
365


 




OPR-17
CCGTACGTAG
366


 




OPR-18
GGCTTTGCCA
367


 




OPR-19
CCTCCTCATC
368


 




OPR-20
ACGGCAAGGA
369


 



KIT S
OPS-01
CTACTGCGCT
370


 




OPS-02
CCTCTGACTG
371


 




OPS-03
CAGAGGTCCC
372


 




OPS-04
CACCCCCTTG
373


 




OPS-05
TTTGGGGCCT
374


 




OPS-06
GATACCTCGG
375


 




OPS-07
TCCGATGCTG
376


 




OPS-08
TTCAGGGTGG
377


 




OPS-09
TCCTGGTCCC
378


 




OPS-10
ACCGTTCCAG
379


 




OPS-11
AGTCGGGTGG
380


 




OPS-12
CTGGGTGAGT
381


 




OPS-13
GTCGTTCCTG
382


 




OPS-14
AAAGGGGTCC
383


 




OPS-15
CAGTTCACGG
384


 




OPS-16
AGGGGGTTCC
385


 




OPS-17
TGGGGACCAC
386


 




OPS-18
CTGGCGAACT
387


 




OPS-19
GAGTCAGCAG
388


 




OPS-20
TCTGGACGGA
389


 



KIT T
OPT-01
GGGCCACTCA
390


 




OPT-02
GGAGAGACTC
391


 




OPT-03
TCCACTCCTG
392


 




OPT-04
CACAGAGGGA
393


 




OPT-05
GGGTTTGGCA
394


 




OPT-06
CAAGGGCAGA
395


 




OPT-07
GGCAGGCTGT
396


 




OPT-08
AACGGCGACA
397


 




OPT-09
CACCCCTGAG
398


 




OPT-10
CCTTCGGAAG
399


 




OPT-11
TTCCCCGCGA
400


 




OPT-12
GGGTGTGTAG
401


 




OPT-13
AGGACTGCCA
402


 




OPT-14
AATGCCGCAG
403


 




OPT-15
GGATGCCACT
404


 




OPT-16
GGTGAACGCT
405


 




OPT-17
CCAACGTCGT
406


 




OPT-18
GATGCCAGAC
407


 




OPT-19
GTCCGTATGG
408


 




OPT-20
GACCAATGCC
409


 



KIT U
OPU-01
ACGGACGTCA
410


 




OPU-02
CTGAGGTCTC
411


 




OPU-03
CTATGCCGAC
412


 




OPU-04
ACCTTCGGAC
413


 




OPU-05
TTGGCGGCCT
414


 




OPU-06
ACCTTTGCGG
415


 




OPU-07
CCTGCTCATC
416


 




OPU-08
GGCGAAGGTT
417


 




OPU-09
CCACATCGGT
418


 




OPU-10
ACCTCGGCAC
419


 




OPU-11
AGACCCAGAG
420


 




OPU-12
TCACCAGCCA
421


 




OPU-13
GGCTGGTTCC
422


 




OPU-14
TGGGTCCCTC
423


 




OPU-15
ACGGGCCAGT
424


 




OPU-16
CTGCGCTGGA
425


 




OPU-17
ACCTGGGGAG
426


 




OPU-18
GAGGTCCACA
427


 




OPU-19
GTCAGTGCGG
428


 




OPU-20
ACAGCCCCCA
429


 



KIT V
OPV-01
TGACGCATGG
430


 




OPV-02
AGTCACTCCC
431


 




OPV-03
CTCCCTGCAA
432


 




OPV-04
CCCCTCACGA
433


 




OPV-05
TCCGAGAGGG
434


 




OPV-06
ACGCCCAGGT
435


 




OPV-07
GAAGCCAGCC
436


 




OPV-08
GGACGGCGTT
437


 




OPV-09
TGTACCCGTC
438


 




OPV-10
GGACCTGCTG
439


 




OPV-11
CTCGACAGAG
440


 




OPV-12
ACCCCCCACT
441


 




OPV-13
ACCCCCTGAA
442


 




OPV-14
AGATCCCGCC
443


 




OPV-15
CAGTGCCGGT
444


 




OPV-16
ACACCCCACA
445


 




OPV-17
ACCGGCTTGT
446


 




OPV-18
TGGTGGCGTT
447


 




OPV-19
GGGTGTGCAG
448


 




OPV-20
CAGCATGGTC
449


 



KIT W
OPW-01
CTCAGTGTCC
450


 




OPW-02
ACCCCGCCAA
451


 




OPW-03
GTCCGGAGTG
452


 




OPW-04
CAGAAGCGGA
453


 




OPW-05
GGCGGATAAG
454


 




OPW-06
AGGCCCGATG
455


 




OPW-07
CTGGACGTCA
456


 




OPW-08
GACTGCCTCT
457


 




OPW-09
GTGACCGAGT
458


 




OPW-10
TCGCATCCCT
459


 




OPW-11
CTGATGCGTG
460


 




OPW-12
TGGGCAGAAG
461


 




OPW-13
CACAGCGACA
462


 




OPW-14
CTGCTGAGCA
463


 




OPW-15
ACACCGGAAC
464


 




OPW-16
CAGCCTACCA
465


 




OPW-17
GTCCTGGGTT
466


 




OPW-18
TTCAGGGCAC
467


 




OPW-19
CAAAGCGCTC
468


 




OPW-20
TGTGGCAGCA
469


 



KIT X
OPX-01
CTGGGCACGA
470


 




OPX-02
TTCCGCCACC
471


 




OPX-03
TGGCGCAGTG
472


 




OPX-04
CCGCTACCGA
473


 




OPX-05
CCTTTCCCTC
474


 




OPX-06
ACGCCAGAGG
475


 




OPX-07
GAGCGAGGCT
476


 




OPX-08
CAGGGGTGGA
477


 




OPX-09
GGTCTGGTTG
478


 




OPX-10
CCCTAGACTG
479


 




OPX-11
GGAGCCTCAG
480


 




OPX-12
TCGCCAGCCA
481


 




OPX-13
ACGGGAGCAA
482


 




OPX-14
ACAGGTGCTG
483


 




OPX-15
CAGACAAGCC
484


 




OPX-16
CTCTGTTCGG
485


 




OPX-17
GACACGGACC
486


 




OPX-18
GACTAGGTGG
487


 




OPX-19
TGGCAAGGCA
488


 




OPX-20
CCCAGCTAGA
489


 



KIT Y
OPY-01
GTGGCATCTC
490


 




OPY-02
CATCGCCGCA
491


 




OPY-03
ACAGCCTGCT
492


 




OPY-04
GGCTGCAATG
493


 




OPY-05
GGCTGCGACA
494


 




OPY-06
AAGGCTCACC
495


 




OPY-07
AGAGCCGTCA
496


 




OPY-08
AGGCAGAGCA
497


 




OPY-09
AGCAGCGCAC
498


 




OPY-10
CAAACGTGGG
499


 




OPY-11
AGACGATGGG
500


 




OPY-12
AAGCCTGCGA
501


 




OPY-13
GGGTCTCGGT
502


 




OPY-14
GGTCGATCTG
503


 




OPY-15
AGTCGCCCTT
504


 




OPY-16
GGGCCAATGT
505


 




OPY-17
GACGTGGTGA
506


 




OPY-18
GTGGAGTCAG
507


 




OPY-19
TGAGGGTCCC
508


 




OPY-20
AGCCGTGGAA
509


 



KIT Z
OPZ-01
TCTGTGCCAC
510


 




OPZ-02
CCTACGGGGA
511


 




OPZ-03
CAGCACCGCA
512


 




OPZ-04
AGGCTGTGCT
513


 




OPZ-05
TCCCATGCTG
514


 




OPZ-06
GTGCCGTTCA
515


 




OPZ-07
CCAGGAGGAC
516


 




OPZ-08
GGGTGGGTAA
517


 




OPZ-09
CACCCCAGTC
518


 




OPZ-10
CCGACAAACC
519


 




OPZ-11
CTCAGTCGCA
520


 




OPZ-12
TCAACGGGAC
521


 




OPZ-13
GACTAAGCCC
522


 




OPZ-14
TCGGAGGTTC
523


 




OPZ-15
CAGGGCTTTC
524


 




OPZ-16
TCCCCATCAC
525


 




OPZ-17
CCTTCCCACT
526


 




OPZ-18
AGGGTCTGTG
527


 




OPZ-19
GTGCGAGCAA
528


 




OPZ-20
ACTTTGGCGG
529


 



KIT AA
OPAA-01
AGACGGCTCC
530


 




OPAA-02
GAGACCAGAC
531


 




OPAA-03
TTAGCGCCCC
532


 




OPAA-04
AGGACTGCTC
533


 




OPAA-05
GGCTTTAGCC
534


 




OPAA-06
GTGGGTGCCA
535


 




OPAA-07
CTACGCTCAC
536


 




OPAA-08
TCCGCAGTAG
537


 




OPAA-09
AGATGGGCAG
538


 




OPAA-10
TGGTCGGGTG
539


 




OPAA-11
ACCCGACCTG
540


 




OPAA-12
GGACCTCTTG
541


 




OPAA-13
GAGCGTCGCT
542


 




OPAA-14
AACGGGCCAA
543


 




OPAA-15
ACGGAAGCCC
544


 




OPAA-16
GGAACCCACA
545


 




OPAA-17
GAGCCCGACT
546


 




OPAA-18
TGGTCCAGCC
547


 




OPAA-19
TGAGGCGTGT
548


 




OPAA-20
TTGCCTTCGG
549


 



KIT AB
OPAB-01
CCGTCGGTAG
550


 




OPAB-02
GGAAACCCCT
551


 




OPAB-03
TGGCGCACAC
552


 




OPAB-04
GGCACGCGTT
553


 




OPAB-05
CCCGAAGCGA
554


 




OPAB-06
GTGGCTTGGA
555


 




OPAB-07
GTAAACCGCC
556


 




OPAB-08
GTTACGGACC
557


 




OPAB-09
GGGCGACTAC
558


 




OPAB-10
TTCCCTCCCA
559


 




OPAB-11
GTGCGCAATG
560


 




OPAB-12
CCTGTACCGA
561


 




OPAB-13
CCTACCGTGG
562


 




OPAB-14
AAGTGCGACC
563


 




OPAB-15
CCTCCTTCTC
564


 




OPAB-16
CCCGGATGGT
565


 




OPAB-17
TCGCATCCAG
566


 




OPAB-18
CTGGCGTGTC
567


 




OPAB-19
ACACCGATGG
568


 




OPAB-20
CTTCTCGGAC
569


 



KIT AC
OPAC-01
TCCCAGCAGA
570


 




OPAC-02
GTCGTCGTCT
571


 




OPAC-03
CACTGGCCCA
572


 




OPAC-04
ACGGGACCTG
573


 




OPAC-05
GTTAGTGCGG
574


 




OPAC-06
CCAGAACGGA
575


 




OPAC-07
GTGGCCGATG
576


 




OPAC-08
TTTGGGTGCC
577


 




OPAC-09
AGAGCGTACC
578


 




OPAC-10
AGCAGCGAGG
579


 




OPAC-11
CCTGGGTCAG
580


 




OPAC-12
GGCGAGTGTG
581


 




OPAC-13
GACCCGATTG
582


 




OPAC-14
GTCGGTTGTC
583


 




OPAC-15
TGCCGTGAGA
584


 




OPAC-16
CCTCCTACGG
585


 




OPAC-17
CCTGGAGCTT
586


 




OPAC-18
TTGGGGGAGA
587


 




OPAC-19
AGTCCGCCTG
588


 




OPAC-20
ACGGAAGTGG
589


 



KIT AD
OPAD-01
CAAAGGGCGG
590


 




OPAD-02
CTGAACCGCT
591


 




OPAD-03
TCTCGCCTAC
592


 




OPAD-04
GTAGGCCTCA
593


 




OPAD-05
ACCGCATGGG
594


 




OPAD-06
AAGTGCACGG
595


 




OPAD-07
CCCTACTGGT
596


 




OPAD-08
GGCAGGCAAG
597


 




OPAD-09
TCGCTTCTCC
598


 




OPAD-10
AAGAGGCCAG
599


 




OPAD-11
CAATCGGGTC
600


 




OPAD-12
AAGAGGGCGT
601


 




OPAD-13
GGTTCCTCTG
602


 




OPAD-14
GAACGAGGGT
603


 




OPAD-15
TTTGCCCCGT
604


 




OPAD-16
AACGGGCGTC
605


 




OPAD-17
GGCAAACCCT
606


 




OPAD-18
ACGAGAGGCA
607


 




OPAD-19
CTTGGCACGA
608


 




OPAD-20
TCTTCGGAGG
609


 



KIT AE
OPAE-01
TGAGGGCCGT
610


 




OPAE-02
TCGTTCACCC
611


 




OPAE-03
CATAGAGCGG
612


 




OPAE-04
CCAGCACTTC
613


 




OPAE-05
CCTGTCAGTG
614


 




OPAE-06
GGGGAAGACA
615


 




OPAE-07
GTGTCAGTGG
616


 




OPAE-08
CTGGCTCAGA
617


 




OPAE-09
TGCCACGAGG
618


 




OPAE-10
CTGAAGCGCA
619


 




OPAE-11
AAGACCGGGA
620


 




OPAE-12
CCGAGCAATC
621


 




OPAE-13
TGTGGACTGG
622


 




OPAE-14
GAGAGGCTCC
623


 




OPAE-15
TGCCTGGACC
624


 




OPAE-16
TCCGTGCTGA
625


 




OPAE-17
GGCAGGTTCA
626


 




OPAE-18
CTGGTGCTGA
627


 




OPAE-19
GACAGTCCCT
628


 




OPAE-20
TTGACCCCAG
629


 



KIT AF 
OPAF-01
CCTACACGGT
630


 




OPAF-02
CAGCCGAGAA
631


 




OPAF-03
GAAGGAGGCA
632


 




OPAF-04
TTGCGGCTGA
633


 




OPAF-05
CCCGATCAGA
634


 




OPAF-06
CCGCAGTCTG
635


 




OPAF-07
GGAAAGCGTC
636


 




OPAF-08
CTCTGCCTGA
637


 




OPAF-09
CCCCTCAGAA
638


 




OPAF-10
GGTTGGAGAC
639


 




OPAF-11
ACTGGGCCTC
640


 




OPAF-12
GACGCAGCTT
641


 




OPAF-13
CCGAGGTGAC
642


 




OPAF-14
GGTGCGCACT
643


 




OPAF-15
CACGAACCTC
644


 




OPAF-16
TCCCGGTGAG
645


 




OPAF-17
TGAACCGAGG
646


 




OPAF-18
GTGTCCCTCT
647


 




OPAF-19
GGACAAGCAG
648


 




OPAF-20
CTCCGCACAG
649


 



KIT AG
OPAG-01
CTACGGCTTC
650


 




OPAG-02
CTGAGGTCCT
651


 




OPAG-03
TGCGGGAGTG
652


 




OPAG-04
GGAGCGTACT
653


 




OPAG-05
CCCACTAGAC
654


 




OPAG-06
GGTGGCCAAG
655


 




OPAG-07
CACAGACCTG
656


 




OPAG-08
AAGAGCCCTC
657


 




OPAG-09
CCGAGGGGTT
658


 




OPAG-10
ACTGCCCGAC
659


 




OPAG-11
TTACGGTGGG
660


 




OPAG-12
CTCCCAGGGT
661


 




OPAG-13
GGCTTGGCGA
662


 




OPAG-14
CTCTCGGCGA
663


 




OPAG-15
CCCACACGCA
664


 




OPAG-16
CCTGCGACAG
665


 




OPAG-17
AGCGGAAGTG
666


 




OPAG-18
GTGGGCATAC
667


 




OPAG-19
AGCCTCGGTT
668


 




OPAG-20
TGCGCTCCTC
669


 



KIT AH
OPAH-01
TCCGCAACCA
670


 




OPAH-02
CACTTCCGCT
671


 




OPAH-03
GGTTACTGCC
672


 




OPAH-04
CTCCCCAGAC
673


 




OPAH-05
TTGCAGGCAG
674


 




OPAH-06
GTAAGCCCCT
675


 




OPAH-07
CCCTACGGAG
676


 




OPAH-08
TTCCCGTGCC
677


 




OPAH-09
AGAACCGAGG
678


 




OPAH-10
GGGATGACCA
679


 




OPAH-11
TCCGCTGAGA
680


 




OPAH-12
TCCAACGGCT
681


 




OPAH-13
TGAGTCCGCA
682


 




OPAH-14
TGTGGCCGAA
683


 




OPAH-15
CTACAGCGAG
684


 




OPAH-16
CAAGGTGGGT
685


 




OPAH-17
CAGTGGGGAG
686


 




OPAH-18
GGGCTAGTCA
687


 




OPAH-19
GGCAGTTCTC
688


 




OPAH-20
GGAAGGTGAG
689


 



KIT AI
OPAI-01
GGCATCGGCT
690


 




OPAI-02
AGCCGTTCAG
691


 




OPAI-03
GGGTCCAAAG
692


 




OPAI-04
CTATCCTGCC
693


 




OPAI-05
GTCGTAGCGG
694


 




OPAI-06
TGCCGCACTT
695


 




OPAI-07
ACGAGCATGG
696


 




OPAI-08
AAGCCCCCCA
697


 




OPAI-09
TCGCTGGTGT
698


 




OPAI-10
TCGGGGCATC
699


 




OPAI-11
ACGGCGATGA
700


 




OPAI-12
GACGCGAACC
701


 




OPAI-13
ACGCTGCGAC
702


 




OPAI-14
TGGTGCACTC
703


 




OPAI-15
GACACAGCCC
704


 




OPAI-16
AAGGCACGAG
705


 




OPAI-17
CCTCACGTCC
706


 




OPAI-18
TCGCGGAACC
707


 




OPAI-19
GGCAAAGCTG
708


 




OPAI-20
CCTGTTCCCT
709


 



KIT AJ
OPAJ-01
ACGGGTCAGA
710


 




OPAJ-02
TCGCACAGTC
711


 




OPAJ-03
AGCACCTCGT
712


 




OPAJ-04
GAATGCGACC
713


 




OPAJ-05
CAGCGTTGCC
714


 




OPAJ-06
GTCGGAGTGG
715


 




OPAJ-07
CCCTCCCTAA
716


 




OPAJ-08
GTGCTCCCTC
717


 




OPAJ-09
ACGGCACGCA
718


 




OPAJ-10
GTTACCGCGA
719


 




OPAJ-11
GAACGCTGCC
720


 




OPAJ-12
CAGTTCCCGT
721


 




OPAJ-13
CAGCCGTTCC
722


 




OPAJ-14
ACCGATGCTG
723


 




OPAJ-15
GAATCCGGCA
724


 




OPAJ-16
TCTGGACCGA
725


 




OPAJ-17
ACCCCCTATG
726


 




OPAJ-18
GGCTAGGTGG
727


 




OPAJ-19
ACAGTGGCCT
728


 




OPAJ-20
ACACGTGGTC
729


 



KIT AK
OPAK-01
TCTGCTACGG
730


 




OPAK-02
CCATCGGAGG
731


 




OPAK-03
GGTCCTACCA
732


 




OPAK-04
AGGGTCGGTC
733


 




OPAK-05
GATGGCAGTC
734


 




OPAK-06
TCACGTCCCT
735


 




OPAK-07
CTTGGGGGAC
736


 




OPAK-08
CCGAAGGGTG
737


 




OPAK-09
AGGTCGGCGT
738


 




OPAK-10
CAAGCGTCAC
739


 




OPAK-11
CAGTGTGCTC
740


 




OPAK-12
AGTGTAGCCC
741


 




OPAK-13
TCCCACGAGT
742


 




OPAK-14
CTGTCATGCC
743


 




OPAK-15
ACCTGCCGTT
744


 




OPAK-16
CTGCGTGCTC
745


 




OPAK-17
CAGCGGTCAC
746


 




OPAK-18
ACCCGGAAAC
747


 




OPAK-19
TCGCAGCGAG
748


 




OPAK-20
TGATGGCGTC
749


 



KIT AL
OPAL-01
TGTGACGAGG
750


 




OPAL-02
ACCCTGTGGG
751


 




OPAL-03
CCCACCCTTG
752


 




OPAL-04
ACAACGGTCC
753


 




OPAL-05
GACTGCGCCA
754


 




OPAL-06
AAGCGTCCTC
755


 




OPAL-07
CCGTCCATCC
756


 




OPAL-08
GTCGCCCTCA
757


 




OPAL-09
CAGCGAGTAG
758


 




OPAL-10
AAGGCCCCTG
759


 




OPAL-11
GTCACGTCCT
760


 




OPAL-12
CCCAGGCTAC
761


 




OPAL-13
GAATGGCACC
762


 




OPAL-14
TCGCTCCGTT
763


 




OPAL-15
AGGGGACACC
764


 




OPAL-16
CTTTCGAGGG
765


 




OPAL-17
CCGCAAGTGT
766


 




OPAL-18
GGAGTGGACT
767


 




OPAL-19
TCTGCCAGTG
768


 




OPAL-20
AGGAGTCGGA
769


 



KIT AM
OPAM-01
TCACGTACGG
770


 




OPAM-02
ACTTGACGGG
771


 




OPAM-03
CTTCCCTGTG
772


 




OPAM-04
GAGGGACCTC
773


 




OPAM-05
GGGCTATGCC
774


 




OPAM-06
CTCGGGATGT
775


 




OPAM-07
AACCGCGGCA
776


 




OPAM-08
ACCACGAGTG
777


 




OPAM-09
TGCCGGTTCA
778


 




OPAM-10
CAGACCGACC
779


 




OPAM-11
AGATGCGCGG
780


 




OPAM-12
TCTCACCGTC
781


 




OPAM-13
CACGGCACAA
782


 




OPAM-14
TGGTTGCGGA
783


 




OPAM-15
GATGCGATGG
784


 




OPAM-16
TGGCGGTTTG
785


 




OPAM-17
CCTAACGTCC
786


 




OPAM-18
ACGGGACTCT
787


 




OPAM-19
CCAGGTCTTC
788


 




OPAM-20
ACCAACCAGG
789


 



KIT AN
OPAN-01
ACTCCACGTC
790


 




OPAN-02
CACCGCAGTT
791


 




OPAN-03
AGCCAGGCTG
792


 




OPAN-04
GGCGTAAGTC
793


 




OPAN-05
GGGTGCAGTT
794


 




OPAN-06
GGGAACCCGT
795


 




OPAN-07
TCGCTGCGGA
796


 




OPAN-08
AAGGCTGCTG
797


 




OPAN-09
GGGGGAGATG
798


 




OPAN-10
CTGTGTGCTC
799


 




OPAN-11
GTCCATGCAG
800


 




OPAN-12
AACGGCGGTC
801


 




OPAN-13
CTTCCAGGAC
802


 




OPAN-14
AGCCGGGTAA
803


 




OPAN-15
TGATGCCGCT
804


 




OPAN-16
GTGTCGAGTC
805


 




OPAN-17
TCAGCACAGG
806


 




OPAN-18
TGTCCTGCGT
807


 




OPAN-19
ACCACGCCTT
808


 




OPAN-20
GAGTCCTCAC
809


 



KIT AO
OPAO-01
AAGACGACGG
810


 




OPAO-02
AATCCGCTGG
811


 




OPAO-03
AGTCGGCCCA
812


 




OPAO-04
AACAGGGCAG
813


 




OPAO-05
TGGAAGCACC
814


 




OPAO-06
AGGCAGCCTG
815


 




OPAO-07
GATGCGACGG
816


 




OPAO-08
ACTGGCTCTC
817


 




OPAO-09
CCAGATGGGG
818


 




OPAO-10
GACATCGTCC
819


 




OPAO-11
GGGGGCTTGA
820


 




OPAO-12
TCCCGGTCTC
821


 




OPAO-13
CCCACAGGTG
822


 




OPAO-14
CTACTGGGGT
823


 




OPAO-15
GAAGGCTCCC
824


 




OPAO-16
CACAACGGGA
825


 




OPA0-17
CCCATGTGTG
826


 




OPA0-18
GGGAGCGCTT
827


 




OPA0-19
GTTCTCGGAC
828


 




OPA0-20
GGCTTGCCTG
829


 



KIT AP
OPAP-01
AACTGGCCCC
830


 




OPAP-02
TGGTCATCCC
831


 




OPAP-03
GTAAGGCGCA
832


 




OPAP-04
CTCTTGGGCT
833


 




OPAP-05
GACTTCAGGG
834


 




OPAP-06
GTCACGTCTC
835


 




OPAP-07
ACCACCCGCT
836


 




OPAP-08
ACCCCCACAC
837


 




OPAP-09
GTGGTCCAGA
838


 




OPAP-10
TGGGTGATCC
839


 




OPAP-11
CTGGCTTCTG
840


 




OPAP-12
GTCTTACCCC
841


 




OPAP-13
TGAAGCCCCT
842


 




OPAP-14
TGCCATGCTG
843


 




OPAP-15
GGGTTGGAAG
844


 




OPAP-16
GGGCAGATAC
845


 




OPAP-17
ACGGCACTCC
846


 




OPAP-18
GTCGTCGACA
847


 




OPAP-19
GTGTCTGCCT
848


 




OPAP-20
CCCGGATACA
849


 



KIT AQ
OPAQ-01
GGCAGGTGGA
850


 




OPAQ-02
ACCCTCGGAC
851


 




OPAQ-03
GAGGTGTCTG
852


 




OPAQ-04
GACGGCTATC
853


 




OPAQ-05
ACGGAGCTGA
854


 




OPAQ-06
ACGGATCCCC
855


 




OPAQ-07
GGAGTAACGG
856


 




OPAQ-08
TCGGTAGACC
857


 




OPAQ-09
AGTCCCCCTC
858


 




OPAQ-10
CATACCCTCC
859


 




OPAQ-11
GACGCCTCCA
860


 




OPAQ-12
CAGCTCCTGT
861


 




OPAQ-13
GAGTCGGCTG
862


 




OPAQ-14
CCCGTGTAGG
863


 




OPAQ-15
TGCGATGCGA
864


 




OPAQ-16
CCCGGAAGAG
865


 




OPAQ-17
TTCGCCTGTC
866


 




OPAQ-18
GGGAGCGAGT
867


 




OPAQ-19
AGTAGGGCCT
868


 




OPAQ-20
GTGAACGCTC
869


 



KIT AR
OPAR-01
CCATTCCGAG
870


 




OPAR-02
CACCTGCTGA
871


 




OPAR-03
GTGAGGCGCA
872


 




OPAR-04
CCAGGAGAAG
873


 




OPAR-05
CATACCTGCC
874


 




OPAR-06
TGGGGCTCAA
875


 




OPAR-07
TCCTTCGGTG
876


 




OPAR-08
GTGAATGCGG
877


 




OPAR-09
GGGGTGTTCT
878


 




OPAR-10
TGGGGCTGTC
879


 




OPAR-11
GGGAAGACGG
880


 




OPAR-12
GGATCGTCGG
881


 




OPAR-13
GGGTCGGCTT
882


 




OPAR-14
CTCACAGCAC
883


 




OPAR-15
ACACTCTGCC
884


 




OPAR-16
CCTTGCGCCT
885


 




OPAR-17
CCACCACGAC
886


 




OPAR-18
CTACCGGCAC
887


 




OPAR-19
CTGATCGCGG
888


 




OPAR-20
TGCGCCATCC
889


 



KIT AS
OPAS-01
CACACCGTGT
890


 




OPAS-02
GTCCTCGTGT
891


 




OPAS-03
ACGGTTCCAC
892


 




OPAS-04
GTCTTGGGCA
893


 




OPAS-05
GTCACCTGCT
894


 




OPAS-06
GGCGCGTTAG
895


 




OPAS-07
GACGAGCAGG
896


 




OPAS-08
GGCTGCCAGT
897


 




OPAS-09
TGGAGTCCCC
898


 




OPAS-10
CCCGTCTACC
899


 




OPAS-11
ACCGTGCCGT
900


 




OPAS-12
TGACCAGGCA
901


 




OPAS-13
CACGGACCGA
902


 




OPAS-14
TCGCAGCGTT
903


 




OPAS-15
CTGCAATGGG
904


 




OPAS-16
AACCCTTCCC
905


 




OPAS-17
AGTTCCGCGA
906


 




OPAS-18
GTTGCGCAGT
907


 




OPAS-19
TGACAGCCCC
908


 




OPAS-20
TCTGCCTGGA
909


 



KIT AT
OPAT-01
CAGTGGTTCC
910


 




OPAT-02
CAGGTCTAGG
911


 




OPAT-03
GACTGGGAGG
912


 




OPAT-04
TTGCCTCGCC
913


 




OPAT-05
ACACCTGCCA
914


 




OPAT-06
CCGTCCCTGA
915


 




OPAT-07
ACTGCGACCA
916


 




OPAT-08
TCCTCGTGGG
917


 




OPAT-09
CCGTTAGCGT
918


 




OPAT-10
ACCTCCGGTC
919


 




OPAT-11
CCAGATCTCC
920


 




OPAT-12
CTGCCTAGCC
921


 




OPAT-13
CTGGTGGAAG
922


 




OPAT-14
GTGCCGCACT
923


 




OPAT-15
TGACGCACGG
924


 




OPAT-16
CTCTCCGTAG
925


 




OPAT-17
AGCGACTGCT
926


 




OPAT-18
CCAGCTGTGA
927


 




OPAT-19
ACCAAGGCAC
928


 




OPAT-20
ACATCAGCCC
929


 



KIT AU
OPAU-01
GGGATGGAAC
930


 




OPAU-02
CCAACCCGCA
931


 




OPAU-03
ACGAAACGGG
932


 




OPAU-04
GGCTTCTGTC
933


 




OPAU-05
GAGCTACCGT
934


 




OPAU-06
TCTCTAGGGG
935


 




OPAU-07
AGACCCTTGG
936


 




OPAU-08
CACCGATCCA
937


 




OPAU-09
ACGGCCAATC
938


 




OPAU-10
GGCGTATGGT
939


 




OPAU-11
CTTCTCGGTC
940


 




OPAU-12
CCACTCGTGT
941


 




OPAU-13
CCAAGCACAC
942


 




OPAU-14
CACCTCGACC
943


 




OPAU-15
TGCTGACGAC
944


 




OPAU-16
TCTTAGGCGG
945


 




OPAU-17
TTGGCATCCC
946


 




OPAU-18
CACCACTAGG
947


 




OPAU-19
AGCCTGGGGA
948


 




OPAU-20
GTCGAAACCC
949


 



KIT AV
OPAV-01
TGAGGGGGAA
950


 




OPAV-02
TCACCGTGTC
951


 




OPAV-03
TGTAGCCGTG
952


 




OPAV-04
TCTGCCATCC
953


 




OPAV-05
GTGAGCGTGG
954


 




OPAV-06
CCCGAGATCC
955


 




OPAV-07
CTACCAGGGA
956


 




OPAV-08
TGAGAAGCGG
957


 




OPAV-09
GAGGTCCTAC
958


 




OPAV-10
ACCCCTGGCA
959


 




OPAV-11
GACCCCGACA
960


 




OPAV-12
AGCCGTCGAA
961


 




OPAV-13
CTGACTTCCC
962


 




OPAV-14
CTCCGGATCA
963


 




OPAV-15
GGCAGCAGGT
964


 




OPAV-16
GACAAGGACC
965


 




OPAV-17
CTCGAACCCC
966


 




OPAV-18
TTGCTCACGG
967


 




OPAV-19
CTCGATCACC
968


 




OPAV-20
TCATGCGCAC
969


 



KIT AW
OPAW-01
ACCTAGGGGA
970


 




OPAW-02
TCGCAGGTTC
971


 




OPAW-03
CCATGCGGAG
972


 




OPAW-04
AGGAGCGACA
973


 




OPAW-05
CTGCTTCGAG
974


 




OPAW-06
TTTGGGCCCC
975


 




OPAW-07
AGCCCCCAAG
976


 




OPAW-08
CTGTCTGTGG
977


 




OPAW-09
ACTGGGTCGG
978


 




OPAW-10
GGTGTTTGCC
979


 




OPAW-11
CTGCCACGAG
980


 




OPAW-12
GAGCAAGGCA
981


 




OPAW-13
CTACGATGCC
982


 




OPAW-14
GGTTCTGCTC
983


 




OPAW-15
CCAGTCCCAA
984


 




OPAW-16
TTACCCCGCT
985


 




OPAW-17
TGCTGCTGCC
986


 




OPAW-18
GGCGCAACTG
987


 




OPAW-19
GGACACAGAG
988


 




OPAW-20
TGTCCTAGCC
989


 



KIT AX
OPAX-01
GTGTGCCGTT
990


 




OPAX-02
GGGAGGCAAA
991


 




OPAX-03
CCAAGAGGCT
992


 




OPAX-04
TCCCCAGGAG
993


 




OPAX-05
AGTGCACACC
994


 




OPAX-06
AGGCATCGTG
995


 




OPAX-07
ACGCGACAGA
996


 




OPAX-08
AGTATGGCGG
997


 




OPAX-09
GGAAGTCCTG
998


 




OPAX-10
CCAGGCTGAC
999


 




OPAX-11
TGATTGCGGG
1000


 




OPAX-12
GGTCGGGTCA
1001


 




OPAX-13
GAGCACTGCT
1002


 




OPAX-14
CACGGGCTTG
1003


 




OPAX-15
CAGCAATCCC
1004


 




OPAX-16
GTCTGTGCGG
1005


 




OPAX-17
TGGGCTCTGG
1006


 




OPAX-18
GTGTGCAGTG
1007


 




OPAX-19
CCCTGTCGCA
1008


 




OPAX-20
ACACTCGGCA
1009


 



KIT AY
OPAY-01
GTCCACCTCT
1010


 




OPAY-02
TGCGAAGGCT
1011


 




OPAY-03
TTTCCGGGAG
1012


 




OPAY-04
AAGGCTCGAC
1013


 




OPAY-05
TCGCTGCGTT
1014


 




OPAY-06
GGCTTCGCAA
1015


 




OPAY-07
GACCGTCTGT
1016


 




OPAY-08
AGGCTTCCCT
1017


 




OPAY-09
CCGATCCAAC
1018


 




OPAY-10
CAAGGCCCCT
1019


 




OPAY-11
ACGCGCCTTC
1020


 




OPAY-12
CTGTCGGCGT
1021


 




OPAY-13
CCGCTCGTAA
1022


 




OPAY-14
GGTGGGTAGA
1023


 




OPAY-15
CCAAGAGGCA
1024


 




OPAY-16
GGTGTGGTTC
1025


 




OPAY-17
GGTGATTCGG
1026


 




OPAY-18
ACCCCAACCA
1027


 




OPAY-19
AACTTGGCCC
1028


 




OPAY-20
TCATTCGCCC
1029


 



KIT AZ
OPAZ-01
TCGGATCCGT
1030


 




OPAZ-02
CCTGAACGGA
1031


 




OPAZ-03
GGCTGTGTGG
1032


 




OPAZ-04
CCAGCCTCAG
1033


 




OPAZ-05
TCCGCATACC
1034


 




OPAZ-06
CCTTCGGAGG
1035


 




OPAZ-07
CACGAGTCTC
1036


 




OPAZ-08
TCGCTCGTAG
1037


 




OPAZ-09
CCTTGACCCC
1038


 




OPAZ-10
ACTCTGGGGA
1039


 




OPAZ-11
TCCAGCGCGT
1040


 




OPAZ-12
GATGGGCCTG
1041


 




OPAZ-13
CCCGAAGCAA
1042


 




OPAZ-14
CACGGCTTCC
1043


 




OPAZ-15
TCCGCTAGTC
1044


 




OPAZ-16
AGGCGAACTG
1045


 




OPAZ-17
CACGCAGATG
1046


 




OPAZ-18
CCGACGTTGA
1047


 




OPAZ-19
ACACTCTCGG
1048


 




OPAZ-20
CATCACCCCT
1049


 



KIT BA
OPBA-01
TTCCCCACCC
1050


 




OPBA-02
TGCTCGGCTC
1051


 




OPBA-03
GTGCGAGAAC
1052


 




OPBA-04
TCCTAGGCTC
1053


 




OPBA-05
TGCGTTCCAC
1054


 




OPBA-06
GGACGACCGT
1055


 




OPBA-07
GGGTCGCATC
1056


 




OPBA-08
CCACAGCCGA
1057


 




OPBA-09
GGAACTCCAC
1058


 




OPBA-10
GGACGTTGAG
1059


 




OPBA-11
CCACCTTCAG
1060


 




OPBA-12
TGTTGGGCAC
1061


 




OPBA-13
AGGGCGAATG
1062


 




OPBA-14
TCGGGAGTGG
1063


 




OPBA-15
GAAGACCTGG
1064


 




OPBA-16
CCACGCATCA
1065


 




OPBA-17
TGTACCCCTG
1066


 




OPBA-18
CTCGGATGTC
1067


 




OPBA-19
CCATCCGTTG
1068


 




OPBA-20
GAGCGCTACC
1069


 



KIT BB
OPBB-01
ACACTGGCTG
1070


 




OPBB-02
CCCCCGTTAG
1071


 




OPBB-03
TCACGTGGCT
1072


 




OPBB-04
ACCAGGTCAC
1073


 




OPBB-05
GGGCCGAACA
1074


 




OPBB-06
CTGAAGCTGG
1075


 




OPBB-07
GAAGGCTGGG
1076


 




OPBB-08
TCGTCGAAGG
1077


 




OPBB-09
AGGCCGGTCA
1078


 




OPBB-10
ACTTGCCTGG
1079


 




OPBB-11
TGCGGGTTCC
1080


 




OPBB-12
TTCGGCCGAC
1081


 




OPBB-13
CTTCGGTGTG
1082


 




OPBB-14
GTGGGACCTG
1083


 




OPBB-15
AAGTGCCCTG
1084


 




OPBB-16
TCGGCACCGT
1085


 




OPBB-17
ACACCGTGCC
1086


 




OPBB-18
CAACCGGTCT
1087


 




OPBB-19
TTGCGGACAG
1088


 




OPBB-20
CCAGGTGTAG
1089


 



KIT BC
OPBC-01
CCTTCGGCTC
1090


 




OPBC-02
ACAGTAGCGG
1091


 




OPBC-03
GGCTTGACCT
1092


 




OPBC-04
CCACGTGCCA
1093


 




OPBC-05
GAGGCGATTG
1094


 




OPBC-06
GAAGGCGAGA
1095


 




OPBC-07
TGTGCCTGAC
1096


 




OPBC-08
GGTCTTCCCT
1097


 




OPBC-09
GTCATGCGAC
1098


 




OPBC-10
AACGTCGAGG
1099


 




OPBC-11
TTTTGCCCCC
1100


 




OPBC-12
CCTCCACCAG
1101


 




OPBC-13
CCTGGCACAG
1102


 




OPBC-14
GGTCCGACGA
1103


 




OPBC-15
CCAGACTCCA
1104


 




OPBC-16
CTGGTGCTCA
1105


 




OPBC-17
CCGTTAGTCC
1106


 




OPBC-18
GTGAAGGAGG
1107


 




OPBC-19
ACAAGCGCGA
1108


 




OPBC-20
AGCACTGGGG
1109


 



KIT BD
OPBD-01
TCACTCGCTC
1110


 




OPBD-02
CCTCCCCAAG
1111


 




OPBD-03
GAGCCCCGAA
1112


 




OPBD-04
TCGGGTGTTG
1113


 




OPBD-05
GTGCGGAGAG
1114


 




OPBD-06
AAGCTGGCGT
1115


 




OPBD-07
GAGCTGGTCC
1116


 




OPBD-08
CATACGGGCT
1117


 




OPBD-09
CCACGGTCAG
1118


 




OPBD-10
GACGCTATGG
1119


 




OPBD-11
CAACCGAGTC
1120


 




OPBD-12
GGGAACCGTC
1121


 




OPBD-13
CCTGGAACGG
1122


 




OPBD-14
TCCCTGTGAG
1123


 




OPBD-15
TGTCGTGGTC
1124


 




OPBD-16
GAACTCCCAG
1125


 




OPBD-17
GTTCGCTCCC
1126


 




OPBD-18
ACGCACACTC
1127


 




OPBD-19
GGTTCCTCTC
1128


 




OPBD-20
AGGCGGCACA
1129


 



KIT BE
OPBE-01
CACTCCTGGT
1130


 




OPBE-02
ACGCCTGTAG
1131


 




OPBE-03
TGGACTCGGT
1132


 




OPBE-04
CCCAAGCGAA
1133


 




OPBE-05
GGAACGCTAC
1134


 




OPBE-06
CAGCGGGTCA
1135


 




OPBE-07
CCGTCCTATG
1136


 




OPBE-08
GGGAAGCGTC
1137


 




OPBE-09
CCCGCTTTCC
1138


 




OPBE-10
AAGCGGCCCT
1139


 




OPBE-11
GTCCTGCTGT
1140


 




OPBE-12
GGTTGTTCCC
1141


 




OPBE-13
TCGGTGAGTC
1142


 




OPBE-14
CTTTGCGCAC
1143


 




OPBE-15
TTCGGCGATG
1144


 




OPBE-16
CTCCACGACT
1145


 




OPBE-17
GGGAAAAGCC
1146


 




OPBE-18
CCAAGCCGTC
1147


 




OPBE-19
AGGCCAACAG
1148


 




OPBE-20
CAAAGGCGTG
1149


 



KIT BF
OPBF-01
GGAGCTGACT
1150


 




OPBF-02
GACACACTCC
1151


 




OPBF-03
TCCCTTGACC
1152


 




OPBF-04
GACAGGTTGG
1153


 




OPBF-05
CACCCCGAAA
1154


 




OPBF-06
TCCACGGGCA
1155


 




OPBF-07
CACCATCGTG
1156


 




OPBF-08
CCTGGGTCCA
1157


 




OPBF-09
ACCCAGGTTG
1158


 




OPBF-10
GTGACCAGAG
1159


 




OPBF-11
GACGACCGCA
1160


 




OPBF-12
CTTCGCTGTC
1161


 




OPBF-13
CCGCCGGTAA
1162


 




OPBF-14
CCGCGTTGAG
1163


 




OPBF-15
ACGCGAACCT
1164


 




OPBF-16
AGGGTCCGTG
1165


 




OPBF-17
CAAGCTCGTG
1166


 




OPBF-18
AGCCAAGGAC
1167


 




OPBF-19
TTCCCGCACT
1168


 




OPBF-20
ACCCTGAGGA
1169


 



KIT BG
OPBG-01
GTGGCTCTCC
1170


 




OPBG-02
GGAAAGCCCA
1171


 




OPBG-03
GTGCCACTTC
1172


 




OPBG-04
GTTCCCGACA
1173


 




OPBG-05
CAAGCCGTGA
1174


 




OPBG-06
GTGGATCGTC
1175


 




OPBG-07
CAGAGGTTCC
1176


 




OPBG-08
GACCAGAGGT
1177


 




OPBG-09
GGCTCTGGGT
1178


 




OPBG-10
GGGATAAGGG
1179


 




OPBG-11
ACGGCAATGG
1180


 




OPBG-12
CCCGAGAAAC
1181


 




OPBG-13
GGTTGGGCCA
1182


 




OPBG-14
GACCAGCCCA
1183


 




OPBG-15
ACGGGAGAGA
1184


 




OPBG-16
TGCTTGGGTG
1185


 




OPBG-17
TCCGGGACTC
1186


 




OPBG-18
TGGCGCTGGT
1187


 




OPBG-19
GGTCTCGCTC
1188


 




OPBG-20
TGGTACCTGG
1189


 



KIT BH
OPBH-01
CCGACTCTGG
1190


 




OPBH-02
GTAAGCCGAG
1191


 




OPBH-03
GGAGCAGCAA
1192


 




OPBH-04
ACCTGCCAAC
1193


 




OPBH-05
GTAGGTCGCA
1194


 




OPBH-06
TCGTGGCACA
1195


 




OPBH-07
TGTACGGCAC
1196


 




OPBH-08
ACGGAGGCAG
1197


 




OPBH-09
GTCTTCCGTC
1198


 




OPBH-10
GTGTGCCTGG
1199


 




OPBH-11
AGCCCAAAGG
1200


 




OPBH-12
TCGCCTTGTC
1201


 




OPBH-13
AGTTGGGCAG
1202


 




OPBH-14
ACCGTGGGTG
1203


 




OPBH-15
GAGAACGCTG
1204


 




OPBH-16
CTGCGGGTTC
1205


 




OPBH-17
CTCTTACGGG
1206


 




OPBH-18
GACGCTTGTC
1207


 




OPBH-19
GTCGTGCGGA
1208


 




OPBH-20
CACCGACATC
1209









DEFINITIONS
BSA—Bulked Segregant Analysis—Selection strategy wherein, in large segregating populations, individuals with the same trait (phenotype) or DNA of these individuals are bulked into “pools”. After screening of these pools with DNA techniques, markers are identified which are linked to the relevant phenotype.
cM—centimorgan—Unit for the genetic distance between markers, based on the number of crossing-overs per hundred individuals.
DNA marker—A DNA fragment which is linked to a gene or another piece of DNA with a known location on the genome, which is used to monitor heritability of this gene or this location.
Dominant—Allel which masks the phenotypical expression of another allel when both are present.
Gel-electrophoresis—Method for separating molecules (DNA, RNA, protein among others), on the basis of their size, shape or charge, in a matrix (agarose or polyacrylamide) under the influence of an electric field.
Gene—The basic unit of heredity, whereby hereditary traits are transmitted from parents to progeny.
Introgression—A chromosome fragment of a line which can for instance be inserted into another line by crossing.
IRDye labels—Labels which are used for Licor imaging systems, the detection of which takes place at 700 nm or 800 nm.
iSSR-primer (inter Simple Sequence Repeat primer)—A primer designed on the 5′ end of an SSR (Single Sequence Repeat), a piece of DNA consisting of a repetition of 2 or 3 nucleotides.
Monogenic—Determined by a single gene.
PCR (Polymerase Chain Reaction)—An in vitro amplification method for multiplying a specific DNA fragment. This synthesis reaction makes use of a minimum of one oligonucleotide primer which hybridizes with a piece of DNA, after which a DNA polymerase amplifies the flanking region via successive temperature cycles.
Primer—A short oligonucleotide (−20-50 bp) complementary to the sequence of a single-strand DNA molecule, which serves as starting point of a polymerase.
RAPD-primer (Random Amplified Polymorphic DNA primer)—A 10-mer with a “random” sequence, wherein the GC-content lies between 60% and 70% and wherein the primer ends are not self-complementary.
RAMPs (Random Amplified Microsatellite Polymorphisms)-DNA fingerprinting technique based on RAPD and iSSR primers with which polymorphisms between different DNA monsters are detected.
Resistance—The ability of a plant to wholly or partially prevent the effects and/or growth of a pathogen.
BC (Backcrossing)—Crossing of an individual with one of the original parents.

## Claims

1. A Brassica oleracea plant, comprising a single dominant resistance gene to Albugo candida, wherein the resistance gene comes from the B. oleracea plant line 9002757, the seeds of said line were deposited in the American Type Culture Collection (ATCC) under number PTA-7412, and wherein the presence of the single dominant resistance gene is demonstrated using at least one DNA marker linked to the resistance gene; wherein the at least one DNA marker is selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:9.

2. The plant as claimed in claim 1, wherein the resistance gene is present in heterozygous form.

3. The plant as claimed in claim 1, wherein the resistance gene is present in homozygous form.

4. The plant as claimed in claim 1, wherein the presence of the resistance gene is demonstrated using at least two DNA markers linked to the resistance gene.

5. The plant as claimed in claim 4, wherein the at least two DNA markers enclose the resistance gene.

6. The plant as claimed in claim 1, wherein the presence of the at least one DNA marker in the genome of the plant is demonstrated by polymerase chain reaction (PCR) using (1) a primer sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 9 and (2) a RAPD primer.

7. The plant as claimed in claim 1, wherein the plant is selected from the group consisting of Brassica oleracea convar. botrytis var. botrytis, Brassica oleracea convar. botrytis var. cymosa, Brassica oleracea convar. botrytis var. asparagoides, Brassica oleracea convar. oleracea var. gemnifera, Brassica oleracea convar. capitata var. alba Brassica oleracea convar. capitata var. rubra, Brassica oleracea convar. capitata var. sabauda, Brassica oleracea convar. acephela var. sabellica, Brassica oleracea convar. acephela var. gongyloides and Brassica oleracea var. tronchuda syn. costata.

8. A fruit or plant part from the plant as claimed in claim 1, wherein the fruit or plant part comprises the at least one DNA marker linked to the resistance gene.

9. A method for providing a Brassica oleracea plant with a resistance to Albugo candida, the method comprising
(a) crossing a first B. oleracea plant, which comprises a resistance gene from the B. oleracea plant line 9002757, the seeds of said line were deposited in the ATCC, under number PTA-7412, with a susceptible second B. oleracea plant to produce progeny plants;
(b) detecting in the progeny plants the presence of one or more DNA markers linked to the resistance gene, wherein the DNA marker is selected from the group consisting of SEQ ID NO: 1 to 9; and
(c) selecting progeny plants in which the presence of the one or more DNA markers linked to the resistance gene has been demonstrated.

10. The method as claimed in claim 9, wherein the resistance gene is present in heterozygous form.

11. The method as claimed in claim 9, wherein the resistance gene is present in homozygous form.

12. The method as claimed in claim 9, wherein the presence of the resistance gene in the selected plants is confirmed by way of a disease test.

13. The method as claimed in claim 9, wherein the selection of the resistant B. oleracea plant in step (c) comprises selecting a B. oleracea plant which comprises at least two DNA markers linked to the resistance gene, wherein the DNA markers enclose the resistance gene.

14. The method as claimed in claim 9, wherein the susceptible B. oleracea plant is selected from the group consisting of B. oleracea convar. botrytis var. botrytis, B. oleracea convar. botrytis var. cymosa, B. oleracea convar. botrytis var. asparagoides, B. oleracea convar. oleracea var. gemnifera, B. oleracea convar. capitata var. alba, B. oleracea convar. capitata var. rubra, B. oleracea convar. capitata var. sabauda, B. oleracea convar. acephela var. sabellica, B. oleracea convar. acephela var. gongyloides, and B. oleracea var. tronchuda syn. costata.

15. A Brassica oleracea plant resistant to Albugo candida obtained with the method as claimed in claim 9, wherein the plant comprises the one or more DNA markers linked to the resistance gene.

16. A method for identifying a Brassica oleracea plant comprising a resistance gene to Albugo candida, wherein the resistance gene comes from the B. oleracea plant line 9002757, the seeds of said line were deposited in the ATCC under number PTA-7412, the method comprising:
detecting at least one DNA marker linked to the resistance gene to A. candida, wherein the at least one DNA marker is selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:9, and wherein the presence of the at least one DNA marker is demonstrated by PCR using (1) a primer sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 9 and (2) a RAPD primer.

17. The plant as claimed in claim 2, wherein the presence of the resistance gene can be demonstrated using at least two DNA markers linked to the resistance gene.

18. The plants as claimed in claim 3, wherein the presence of the resistance gene is demonstrated using at least two DNA marker markers linked to the resistance gene.

19. The method as claimed in claim 10, wherein the presence of the resistance gene in the selected plants is confirmed by way of a disease test.

20. The method as claimed in claim 11, wherein the presence of the resistance gene in the selected plants is confirmed by way of a disease test.

21. The method as claimed in claim 9, wherein the presence of the one or more DNA markers is demonstrated by PCR using (1) a primer sequence selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:9 and (2) a RAPD primer.