SERI/SP-232-3079a

UC Catogory: 6lc

ADDENDUM TO

MICROALGAE CULTURE COLLECTION

1986 1987

December 1987

Prepared by the

SERI Microalgal Technology

Research Group

J. Johansen, P. Lemke, N. Nagle, P. Chelf,

P. Roessler, R. Galloway and S. Toon

Solar Energy Research Institute

A Division of Midwest Research Institute

1617 Cole Boulevard

Golden, Colorado 80401

Prepared for the

U.S. Department of Energy

Contract No. DE-AC02-83CH10093
NOTICE

This report was prepared as an account of work sponsored by the United States

Government. Neither the United States nor the United States Department of Energy,

nor any of their employees, nor any of their contractors, subcontractors, or their

employees, makes any warranty, express or implied, or assumes any legal liability

or responsibility for the accuracy, completeness or usefulness of any information,

apparatus. product or process disclosed, or represents that its use would not

infringe privately owned rights.
INTRODUCTION

In 1984, the SERI Microalgae Culture Collection was established in support

of the U.S. Department of Energy's Biofuels and Municipal Waste Technology

Program. It provides a repository for strains identified or developed for mass

Culture biomass production and makes these strains readily available to the

research community. The strains in the collection have been selected for their

potential in biomass fuel applications, and many produce significant quantities

of cellular storage lipids.

Two catalogs of the Microalgae Culture Collection have been printed and

distributed free to those requesting them, one for 1985-1986 (Anonymous 1986)

and one for 1986-1987 (Barclay et ale 1986). The 1986-1987 ·catalog is still

current, and this year we plan to print only this addendum to that catalog. We

will list only those species newly characterized and not listed in the 1986-

1987 catalog. This includes a number of species collected as part of the coldwater collection and screening program as well as species collected from warm

waters. Modifications to culture recipes are given. We are also providing a

list of all past names of culture strains along with their current SERI

designation. We ask that all researchers repQrting work on SERl strains use

the current SERI designation.

As in past years, cultures will be shipped free of charge to interested

researchers. Again, we request that investigators using species from this

collection supply us with copies of pertinent publications of their research

with these strains. For explanatory notes on abbreviations see page 2 of the

1986-1987 catalog. Please note that conductivity is now reported in mS/cm

(equivalent to mrnho/cm) since this is the correct SI unit.

All cultures in this addendum and in Microalgae Culture Collection

1986-1987 are available without charge for research and culture applications.

Requests for cultures are accepted by letter, which should be addressed as

follows:

Dr. Jeff Johansen

Microalgae Culture Collection

Solar Energy Research Institute

1617 Cole Blvd.

Golden, CO 80401

Questions about the culture collection or requests for information can be

made by phone to 303-231-1247.

1
Chaetoceros muelleri var. subsalsurn (Lemm.) Johan. and Rush.

Strain: CHAET9

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Centrales

Family: Chaetoceraceae

Collection site: Ephemeral pond near Ludlow Sheep Barns, east shore of Goshen

Bay, Utah Lake, Utah, U.S.A. (S. Rushforth).

Date:

Water temperature:

Salinity:

July 24, 1985

340C

43 mS/cm

Size: valves 3-7 urn wide, 4-12 urn long, pervalvar length 4-10 urn.

Growth form: unicellular, not forming chains or colonies.

Growth rate at optimum: 4.0 doublings/day

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea, nitrate

Suitable media: SERI Type II best, SERI Type I adequate

Nutritional modes: autotrophic

Temperature range: 10-350C

Temperature optimum: 300C

Salinity range: 10-115 mS/cm

Salinity optt.um: 25 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture.

TYPE I TYPE II ASW

70 •

.....

-;;. 55 '0.5

<J

rn

.§

40 •

.s

u

c:

o

U 25 •

. 2·0 .

35

70 •

.....

';" 55 •

E

<J

rn

E...

2

70 •

.....

';" 55 •

E

<J

rn

E... 40 •

.s

u

s

u 25 •

35
Lipid Content:

Nutrient sufficient:

Nitrogen deficient:

Silicon deficient:

Notes Concerning this Strain:

17.8%

27.6%

24.8%

(4 days deficiency)

(2 days deficiency)

Identification has been verified with the observation of resting spores

and consistent absence of valvar processes and chains. This is one of our most

euryhaline strains, growing at 0.70 doublings/day at 115 mS/cm conductivity at

30°C. Growth below 0.50 doublings/day has been observed at 130 mS/cm

conductivity. The site of origin is a playa lake, and is dry for several

months of every year.

3
Chaetoceros muelleri var. muelleri Lemmerman

Strain: CHAET58

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Centrales

Family: Chaetoceraceae

Collection site: Soap Lake, Washington, U.S.A. (J. Johansen).

Date:

Water temperature:

Salinity:

March 6, 1987

70C

11 mS/cm

Size: valves 3-9 urn wide, 3.5-29 urn long, pervalvar length 4-15 urn.

Growth form: chain-forming (up to 20 cells/chain).

Growth rate at optimum: 2.8 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea, nitrate

. Suitable media: SERI Type II best, Artificial seawater adequate

Nutritional modes: autotrophic

Temperature range: 10-350C

'Temperature optimum: 25-30oC

Salinity range: 10-70 mS/cm

Salinity optimum: 25 mS/cm

Temperature/salinity growth responses:

70 •

f 55 •

e

o

en

.§

>0 40 •

e

8 25 •

35

70 •

0.5

55 • Ie

o

en

.§

40 • :~

I

o 25 •

4

70 •

7" 55 •

e

o

en

.§

40 • :~

u

u 25 •
Chaetoceros muelleri yare subsalsum (Lemm.) Johan. and Rush.

Strain: CHAET61

Taxonomy: Diviston: Chrysophyta

Class: Bacillariophyceae

Order: Centrales

Family: Chaetoceraceae

Collection site: Farmington Bay, Great Salt Lake, Utah, U.S.A. (S. Rushforth).

Date:

Water temperature:

Salinity:

August 7, 1986

25°C

38 mS/cm

Size: valves 4-7 urn wide, 5-12 urn long, pervalvar length 5-10 urn.

Growth form: unicelluar, not forming chains.

Growth rate at optimum: 3.3 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea, nitrate

Suitable media: SERI Type II best, SERI Type I adequate

Nutritional modes: autotrophic

Temperature range: 10-350C

~Temperature optimum: 30°C

Salinity range: 10-70 mS/cm

Salinity optt.u.: 10-25 mS/cm, 40 mS/cm in Artificial seawater

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture.

TYPE I TYPE II ASW

15 20 25 30 35

Temperatore (oC)

z:. 40 •

:~

ti

.ae

<3 25 •

10...• ......._

10 15 20 25 30 35

Temperature (oC)

70 •

::- 55 I

E(.J

dJ

E

40 •

~i

g

'0

I::

<3 25

5
Chaetoceros muelleri var. subsalsum (Lemm.) Johan. and Rush.

Strain: CHAET63

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Centrales

Family: Chaetoceraceae

Collection site: Farmington Bay, Great Salt Lake, Utah, U.S.A. (S. Rushforth).

Date:

Water temperature:

Salinity:

September 4, 1986

240C

40 mS/cm

Size: valves 4-7 urn wide, 4-10 urn long, pervalvar length 4-9 urn.

Growth form: unicellular, not forming chains.

Growth rate at optimum: 3.1 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea, nitrate . Suitable media: SERI Type II best, other saline media adequate

Nutritional modes: autotrophic

Te-perature range: 10-350C

'Temperature optimum: 30°C in Type II, 25-30oC in other media

Salinity range: 10-70 mS/cm

Salinity opt~: 25 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture.

TYPE I TYPE U ASW

35

70 •

2.5

10....-L-'-~~~--I~_~'--~

10 15 20 25 0 30 35

Temperature ( C)

-- -I

e

o

:§

>. 40 •

:i

o 25 •

35

1.5

1.0 2.0

70 •

---'e 55 •

c

f/)

.§

.?:' 40 • :~

u

c

6
Cyclotella cryptica Reimann et ale

Strain: CYCL04

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Centrales

Family: Thalassiosiraceae

Collection site: Wet meadow north of Goshen Playa, Utah County, Utah, U.S.A.

(B. Barclay).

Date:

Water temperature:

Salinity:

pH:

May 24, 1985

28°C

25 mS/cm

8.4

Size: valves 6-8 um in diameter.

Growth form: unicellular.

Growth rate at optimum: 1.6 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea, nitrate

Suitable media: Artificial seawater best, SERI Type I adequate

Nutritional modes: autotrophic

Temperature range: 10-350C

Temperature optimum: 30°C

Salinity range: 10-70 mS/cm

Salinity opt~UID: 25 mS/cm

Temperature/salinity growth responses:

15 20 25 30 35

Temperature (0 C)

10 •

15 20 25 30 35 10

Temperature (0C)

10 •

35 10

•

15 20 25 30

Temperature (0 C)

10'-·_--'- ...1- ....

10

Exponential growth rate (doublings/day) in semicontinuous batch culture.

Type I Type II ASvJ

70 • • 70 • • • • • • 70 •

-:- 55 • 55 • • 55 • E

o E E u u . 0.5 1.0 . . 0 0 0 J:; J:; J:; E 40 • • • • • E 40 • E §. §. g 40 • •

0.5 1.0 :~ :~ :~ iii iii iii (/) 25 . • en 25 · en 25 •

7
Lipid Content:

Nutrient sufficient:

Nitrogen deficient:

Silicon defifient:

Notes Concerning this Strain:

12.1%

11.3%

13.2%

(4 days deficiency)

(2 days deficiency)

This strain is physiologically similar to CYCL02. though growth rates are

slightly lower than observed for that strain. CYCL01 is the fastest growing

strain of Cyclotella cryptica in the SERI collection. but researchers

interested in studying physiological variability in this species may wish to

work with more than one strain. Additional uncharacterized strains of C.

cryptica are also available upon request.

8
Amphiprora hyalina Eulenstein

Strain: ENTOM3 (BB-333)

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Pennales

Family: Entomoneidaceae

Collection site: Biloxi Bridge, Biloxi, Mississippi, U.S.A. (M. -Tadros).

Date:

Water temperature:

Salinity:

pH:

June 1986

270 e

24 mS/cm

7.5

Size: valve length 14-27 urn, pervalvar width 9-16 urn, 45-48 striae in 10 urn.

Growth form: unicellular.

Growth rate at optimum: 2.0 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: nitrate best, urea good, ammonium poor

Suitable media: f/2

Nutritional modes: autotrophic

Te-perature range: 20-350e

Temperature optimum: 25-30oC

Salinity range: 10-60 mS/cm

Salinity optiBum: 35-60 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture. Fig.

lA. 80 uE·m-Z·s-1 illumination. Fig. lB. 160 uE·m- 2·s-1 illumination.

Temperatures tested were: 200 e (diamonds), 25°C (triangles), 300 e (circles),

35°C (squares).

4

1

4

3

2

....--...-...--... ,"" 1

/

/

1A 18

1020 3545 60 1020 3545 60

Conductivity (mS/cm)

9
Lipid Content:

Nutrient sufficient:

Nitrogen deficient:

Silicon defifient:

22.1%

30.2%

37.1%

(3 days deficiency)

(3 days deficiency)

Nitrogen Preference:

Exponential growth rate using nitrate (triangles), urea (circles) and ammonium

(squares) as nitrogen source.

4 4

3

2

1

7

•

.•..-....•

..... -' ". .,- .,- ". .. .-.-'-- ..- 1iI"., - - -. v , ...

•...

1

~3

"

<,

en

C'

.5 2

:0

::I

o

Q

.005 .01 1 2

Concentration (mM)

3

Notes Concerning this Strain:

Reimer (Patrick and Reimer 1975) points out that Amphiprora Ehr. is

invalidly described and that species belonging to the genus should be placed in

Entomoneis Ehr. However, in his treatment of Entomoneis he does not transfer

~. hyalina to Entomoneis. We agree with the use of the epithet Entomoneis, but

as this is an inappropriate place to make a nomenclatural change we use

Amphiprora.

10
Unidentified Prasinophyte

Strain: GREEN3

Taxonomy: Division: Chlorophyta

Class: Prasinophyceae

Collection site: Sheep pond, Hwy 83, Box Elder County, Utah, U.S.A. (P. Lemke

and J. Johansen).

Date:

Water temperature:

Salinity:

pH:

October 23, 1986

28°C

38.7 mS/cm

8.9

Size: 7-13 urn wide, 11-18 um long.

Growth form: unicellular, dorsiventrally flattened biflagellate possessing an

apical flagellar pit. Starch producing pyrenoid present.

Growth rate at opt±-um: 2.4 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea

Suitable media: Artificial seawater

Nutritional modes: autotrophic

Temperature range: 10-30oe

Temperature optimum: 250 e

Salinity range: 10-70 mS/cm

Salinity optt.um: 40 mS/cm

Temperature/salinity growth responses:

::-

I

e

u

en

!

• 2.0 •

15 20 25 30 35

Temperature (oC)

10'-·_......--"'-"'-.......----L~-__

10

s: 55 Ie

u

en

.s

:>0 40 •

:~

u

a

o 25 •

15 20 25 30 35

Temperature (oC)

Exponential growth rate (doublings/day) in semicontinuous batch culture.

TYPE I TYPE H ASW

70 .

:=- 55 • I

e

o

en

5

Z' 40 •

:~

u

g

o 25 •

11
Navicula acceptata Hustedt

Strain: NAVIC6

Taxonomy: Division: Chrysophyta

Class: Bacillariophyc€ae

Order: Pennales

Family: Naviculaceae

Collection site: Biloxi Bridge, Siloxi , Mississippi, U.S.A. (M. Tadros).

Date:

Water temperature:

Salinity:

pH:

May 1985

26°C

22 mS/cm

7.8

Size: valves 6-9 urn long, 3-4.5 um wide, 15-16 striae in 10 urn.

Growth form: unicellular.

Growth rate at optimum: 3.8 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: nitrate or ammonium good, urea poor

Suitable media: f/2

Nutritional modes: autotrophic

Temperature range: 20-350C

. Temperature optimum: 35°C

Salinity range: 10-60 mS/cm

Salinity optimum: 20-45 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture. Fig.

1A. 80 uE·m-2·s-1 illumination. Fig. lB. 160 uE·m-2·s-1 illumination.

Temperatures tested were: 250C (triangles), 300C (circles), 35°C (squares).

10 20 3545 60 1020 3545

Conductivity (mS/em)

2

3

4

1

._._ .. _ 4

i •..~, • «: , \'

i: ',~

; ','. I

.;

,.

of

I';

.'iI

,

I

: I

o ,

: I

3A

,

.

N. acceptata

,

,

,

I•

I

I

I

I

•

I

1 I

I

I •

4

>0 3 «l

'0

"-II)

'"

c 2

:c

:::I

o

o

12
Lipid Content:

Nutrient sufficient:

Nitrogen deficient:

Silicon defifient:

Nitrogen Preference:

21.8%

32.4%

48.5%

(3 days deficiency)

(3 days deficiency)

Exponential growth rate using nitrate (triangles), urea (circles) and ammonium

(squares) as nitrogen source.

4

1

N. acceptata

9

.005 .01 1 2 3

Concentration (mM)

4

3

2

1

Notes Concerning this Strain:

NAVIC6 and NAVIC8 have slightly coarser striae than described for N.

acceptata, but other features are typical of the species.

13
Navicula acceptata Hustedt

Strain: NAVIC8 (SB-304)

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Pennales

Family: Naviculaceae

Collection site: St. Joseph Bay, Florida, U.S.A. (M. Tadros).

Date:

Water temperature:

Salinity:

pH:

June 1986

30°C

31 mS/cm

6.2

Size: valves 6-10 um long, 3-4 um wide, 15-16 striae in 10 um.

Growth form: unicellular.

Growth rate at optimum: 3.8 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: nitrate best, ammonium good, urea poor

Suitable media: f/2

Nutritional modes: autotrophic

Temperature range: 20-350C

Temperature optimum: 35°C

Salinity range: 10-60 mS/cm

Salinity opt~: 45-60 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture. Fig.

1A. 80 uE·m-2·s-1 illumination. Fig. lB. 160 uE·m-2·s-1 illumination.

Temperatures tested were: 200C (diamonds), 250C (triangles), 30°C (circles),

35°C (squares).

3

2

4

1

".-.•. _._ .. ;

;

;

; •....•

;

; . ;rr::

,.. ,

!,'--~

': .........----.... .',' ..",.",,-"

.: r" f: /

/

........

N. acceptata

_.... ..- ..-.

,_...-.- I

I

i

i ,•••••••• -_

1 .i ...... I: ...-_ ..... - ...

i,,' ~" '/

4

4A 48

10 20 3545 60 1020 3545 60

Conductivity (m5/cm)

14
Lipid Content:

Nutrient sufficient:

Nitrogen deficient:

Silicon defifient:

19.2%

38.2%

42.5%

(3 days -deficiency)

(3 days deficiency)

Nitrogen Preference:

Exponential growth rate using nitrate (triangles), urea (circles) and ammonium

(squares) as nitrogen source.

1

3

2

4

• • •

rr::

I ,.

,-

i

i

_._._. _.-.---- 4' ,.,. ...._.... _...._._.- ,,~

............ __ ._ .

N. acceptata 4

1

~3

'0

<,

fI)

at

.S 2

D

::J

o

Q

10

.005 .01 1 2 3

Concentration (mM)

15
Navicula saprophila Lange-Bertalot and Bonik

Strain: NAVIC24

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Pennales

Family: Naviculaceae

Collection site: Lake Lenore, Washington, U.S.A. (J. Johansen).

Date:

Water temperature:

Salinity:

March 6, 1987

9°C

1.1 mS/cm

Size: valves 1.8-2.7 urn wide, 6-8 urn long.

Growth form: unicellular.

Growth rate at optimum: 3.1 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea

Suitable media: SERI Type II, Artificial seawater adequate

Nutritional modes: autotrophic

Temperature range: 10-30oC

Temperature optimum: 25-30oC in Type II, 20°C in other media

Salinity range: 10-70 mS/cm

Salinity opt~: 10 mS/cm in Type II, 25 mS/crn in other media

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture.

TYPE I TYPE. ASW

70 •

;:- 55 •

'e

(J

ciJ

oS

>40·

:~

u

c

<3 25 •

35

16

70 •

;:- 55 •

'e

(J

ciJ

oS

> 40 •

u

<3 25 •

35
Nitzschia dissipata (Kuetz.) Grunow

Strain: NITZS13 (SB-307)

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Pennales

Family: Nitzschiaceae

Collection site: St. Joseph Bay, Florida, U.S.A. (M. Tadros).

Date:

Water temperature:

Salinity:

pH:

June 1986

29°C

28 mS/cm

8.2

Size: valves 18-28 x 4-6.5 urn, (6)8-10 fibulae in 10 um, 48-51 striae in 10 urn.

Growth form: unicellular.

Growth rate at optt.um: 2.6 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: nitrate good, urea and ammonium poor

Suitable media: f/2

Nutritional modes: autotrophic

Temperature range: 1S-30oe

Temperature optimum: 250e

Salinity range: 10-60 mS/cm

Salinity opt~: 20 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture. Fig.

1A. 80 uE·m-2·s-1 illumination. Fig. lB. 160 uE·m-2·s-1 illumination.

Temperatures tested were: 2SoC (triangles), 300 e (circles), 3Soe (squares).

4 N. dissipata 4

2

~3 3

"0

<,

(I)

m

.= 2 12

:J

o

Q

1 " .• 1

--~·-··_-··-··_·6A ·-··-..·_·_ ..·-... ·_·~B

1020 3545 60 1020 3545 60

Conductivity (mS/em)

17
Lipid Content:

Nutrient sufficient:

Nitrogen deficient:

Silicon defifient:

Nitrogen Preference:

27.6%

45.9%

47.2%

(3 days deficiency)

(3 days deficiency)

Exponential growth rate using nitrate (triangles), urea (circles) and ammonium

(squares) as nitrogen source.

4

1

N. dissipata

..._.- _._._._._._.- _._~.-

, -, ,

,

,

:

v· _ _-_ ..__ _--~--_.~ ·

~~

12

.005 .01 1 2 3

Concentration (mM)

18

4

3

2

1
Nitzschia communis Rabenhorst

Strain: NITZS28

Taxonomy: Division: Chrysophyta

Class: Bacillariophyceae

Order: Pennales

Family: Nitzschiaceae

Collection site: Soap Lake, Washington, U.S.A. (J. Johansen).

Date:

Water temperature:

Salinity:

March 6, 1987

70C

11 mS/cm

Size: valves 4-5 x 25-27 urn, fibulae 11-12 in 10 urn, striae 30-35 in 10 urn.

Growth form: unicellular.

Growth rate at optimum: 2.7 doublings/day.

Culture conditions:

Vitamins required: not determined

Available nitrogen sources: urea

Suitable media: SERI Type II, other saline media adequate

Nutritional modes: autotrophic

Temperature range: 10-30oC

~. Temperature optimum: 25°C

Salinity range: 10-70 mS/cm

Salinity optiaum: 25 mS/cm

Temperature/salinity growth responses:

Exponential growth rate (doublings/day) in semicontinuous batch culture.

TYPE I TYPE II ASW

10_·__.......__......._--I-.......L.I....-'

10 15 20 25 30 35

Temperature (oe)

10_·...... ......

10 15 20 25 30 35

Temperature (oC)

10_·__......_ ...... __--r..I""'--......

10 15 20 25 30 35

Temperature (oC)

70 70 70

0.5

..... ..... -I 55 , 55 I 55

E E E (J u 9 UJ 0 UJ .§ 0.5 .§ !

:!: 40 .~ 40· :!: 40 • :~ .2: .s ti u ti :I :::l '0 '0 C C c

8 25 0 0 0 25· o 25

19
MODIFICATIONS TO CULTURE MEDIA

Modified PII Trace Element Stock

This trace metal stock is used in making GPM, SERI Type I, and SERI Type

II media. We have modified it by adding Mo, Cu, and Ni. The full recipe is

listed below.

Dissolve in 1 liter H20:

Na2EDTA •.

FeC13'6H20

H3B03 . . • .

MnC12·4H20

ZnCl2 . . .

CoC12'6H20 ...•

NaMo04'2H20

CuSo4' 5H20

NiSo4' 6H20

· 6.00 g

· 0.29 g · . 6.84 g

0.86 g

· 0.06 g

• • 0.026 g

· • 0.005 g

0.002 g

• . 0.052 g

Adjust pH to 7.8-8.0 with NaOH. Keep refrigerated.

Some diatoms have recently been shown to have a selenium requirement,

particularly members of the Thalassiosiraceae (Price al. 1987). We have

observed morphological aberrations identical to those reported for Se-deficient

Thalassiosira pseudonana (Doucette al. 1987) in our cultures of

Thalassiosira weissflogii and Cyclotella cryptica, and recommend the addition

of Se to the PII trace metal stock if these diatoms are being cultured. The

final concentration should be 10-9 M in the culture medium, which can be

achieved by dissolving 35 mg Na2Se03 in 1 liter H20, and then adding 1 ml of

this solution to the PII trace metal stock.

Fe-EDTA Solution

For routine culture maintenance we add 0.060 ml Fe-EDTA solution per liter

of SERI Type I and SERI Type II media. This solution is made by dissolving

26.1 grams of EDTA in 268 ml of IN KOH, to which 24.9 grams FeS04'H20 are

added. Volume is made up to one liter with distilled water. The solution is

bubbled overnight before using.

SERI Type I and Type II Media

For routine culture maintenance and physiological experiments, urea is

used as the nitrogen source. It is added either as 1ml/l of a 0.3 M urea

solution, or directly as 18 mg urea/l. PII trace metals and Fe-EDTA are added

as indicated above. For all diatom cultures, 0.5 g NaZSi03'9HZO is dissolved

in 25 ml distilled water, pH adjusted to 9.3-9.5, and then added to the medium.

Both Type I and Type II media are filter sterilized, with all enrichments

except silica being run through the filter before the salt solution. Silica is

added last while vigorously agitating the flask. Silica solutions should be

mixed just prior to filtration.

20
CURRENT SERI STRAIN DESIGNATIONS

We ask that all researchers use the strain designations listed in the far

left column. Some designations used in the past are listed on the right. For

cultures from other culture collections and/or with a past publication history,

it is probably best to give both designations, the less preferred name "to be

given only once in parentheses. Examples of these strains include UTEX 572

(BOTRYl), ISOCHI (T-I50), and NANNP2 (Nanno-Q).

STRAIN

AMPHOI

AMPH027

AMPH028

ANKISI

BOEKEI

BOTRYl

CHAETI

CHAET6

CHAET9

CHAETlO

CHAET14

CHAET15

CHAET38-

CHAET39-

CHAET58

CHAET59

CHAET6l

CHAET63

CHLORI

CHLOR2

CHLOR3

CYCLOI

CYCL02

CYCL04

ELLIPI

ENTOM3

FRANC1

ISOCHI

MONORI

MONOR2

NANN02

NANN012

NANNPI

NANNP2

NAVlCl

NAVIC2

NAVIC6

NAVIC7

NAVIC8

NAVIC24

SPECIES

Amphora coffeiformis

A. delicatissima

A. coffeiformis

Ankistrodesmus falcatus

Boekelovia hooglandii

Botryococcus braunii

Chaetoceros gracilis

C. muelleri var. subsalsum

C. muelleri yare subsalsum

C. muelleri yare subsalsum

c. muelleri yare subsalsum

c. muelleri (undescribed var.)

C. muelleri yare subsalsum

C. muelleri var. subsalsum

C. muelleri yare muelleri

C. muelleri var. subsalsum

C. muelleri yare subsalsum

C. muelleri yare subsalsum

Chlorella species

C. ellipsoidea

Chlorella species

Cyclotella cryptica

C. cryptica

C. cryptica

Ellipsoidon species

Amphiprora hyalina

Franceia species

Isochrysis aff. galbana

Monoraphidium minutum

M. minutum

Nannochloris species

Nannochloris species

Nannochloropsis salina

Nannochloropsis species

Navicula saprophila

!. saprophila

!. acceptata

!. saprophila !. acceptata

Navicula saprophila

21

PAST DESIGNATIONS

S/AMPHO-l

ASU 0032, MLS...;l

ASU 3001, GR-2

S/ANKIS-l, Pyramid Lake 91-1

S/BOEKE-l

UTEX #572

S/CHAET-l, CHGRA

NM-6

UT-147

S/CHAET-4, UT-27

S/CHAET-2, SS-14

49-lA

SH9-1

UL-7

S/CHLOR-l, SOl

S/CHLOR-2, BL-6

S/CHLOR-3, SC-2

S/CYCLO-l, DI-35

F-l, Paul's Cyclotella

UT-65

NANN013 , 70-01

BB-333

ASU 0146, LCC-l

S/ISOCH-l, Tahititian T-ISO

S/MONOR-l, Mom's Ranch

S/MONOR-2

HB44

120-01

S/NANNO-l, GBSTlCHO

S/NANNO-2, Nanno-Q

Navicula F-2, Paul's Navicula

26-1A

SB-264

BB-260

SB-304
STRAIN

NITZSl

NITZS2

NITZS13

NITZS14

NITZS28

OOCYSI

PHAEOI

PHAE02

PLEURI

TETRA1

TETRA4

THALA2

THALA6

SPECIES

Nitzschia species

Nitzschia dissipata

Nitzschia dissipata

N. communis

N. communis

Oocystis pusilla

Phaeodactylum tricornutum

P. tricornutum

Pleurochrysis carterae var.

dentata

Tetraselmis suecica

Tetraselmis species

Thalassiosira weissflogii

T. weissflogii

22

PAST DESIGNATIONS

S/NITZS-l, Mono Lake Nitzschia

S/NITZS-2, DI-160, Tadros Hantzschia

SB-307

ASU 0665, PV-2

S/OOCYS-l

.S/PHAEO-l, TFX-l

S/PHAEO-2, BB

Pleurochrysis dentata

S/PLATY-l

HB47

CO-F15

SH14-89
REFERENCES

Anonymous. 1986. Microalgae Culture Collection 1985-1986. SERI, Golden, CO,

97 pp.

Barclay, B., J. Johansen, P. Chelf, N. Nagle, P. Roessler and P. Lemke. 1986.

Microalgae Culture Collection 1986-1987. SERI, Golden, CO, 149 pp.

Doucette, G.J., N.M. Price and P.J. Harrison. 1987. Effects of selenium

deficiency on the morphology and ultrastructure of the coastal marine diatom

Thalassiosira pseudonana (Bacillariophyceae). J. Phycol. 23:9-17.

Patrick, R. and C.W. Reimer. 1975. The Diatoms of the United States. Vol. 2,

Part 1. Monographs of the Academy of Natural Sciences-of Philadelphia 13:1-

213.

Price, N.M., P.A Thompson and P.J. Harrison. 1987. Selenium: an essential

element for growth of the coastal marine diatom Thalassiosira pseudonana

(Bacillariophyceae). J. Phycol. 23:1-9.

23