PMN Page 1 ; Page 1 of 40 

FormA 10-31-96. 

U.S. ENVIRONMENTAL PROTECTION AGENCY 

~ 

ftE PA PREMANUFACTURE 

NOTTC'F. 

When 

completed 

send th1s 

lorm to 

11111111111111111111111111111111111~11111111111111111111111111111111111111 5 1 1 0 0 0 0 0 0 0 5 I S 

Enter the total number of pages 

in the Premanufacture Notice I \'1 

Date of receipt 

··. ·:· :.;-·· 

-·· '. f \ ~ .. J 

39 

Company San ·r·. .. IIZed 

~llnmootros EPA case number P-10·05 

GENERAL INSTRUCTIONS TS • You must provide all information requested in this form to the extent that it is known to or reasonably ascertainable by you. Make reasonable estimates if you do 

not have actual data. 

• Before you complete this form, you should read the "Instructions Manual for Premanufacture Notification" (the Instructions Manual is available from the Toxic 

Substances Control Act (TSCA) Information Service by calling 202-554-1404, or faxing 202-554-5603). 

If a user fee has been remitted for this notice (40 CFR 700.45), indicate in the boxes above the TS-user fee identification number you have generated. Remember, 

your user fee ID number must also appear on your corresponding fee remittance, which is sent to EPA, Washington Financial Management Center (3303), P.O. 

360399M, Pittsburgh, PA 15251-6399, Attn. TSCA User fee. 

Part I-GENERAL INFORMATION 

You must provide the currently correct Chemical Abstracts (CA) Name of the new 

chemical substance, even if you claim the identity as confidential. Yon may authorize 

another person to submit chemical identity information for you, but your submission will 

not be complete and the review will not begin until EPA receives this information. A letter 

in support of your submission should reference your TS user fee identification number. 

You must submit an original and two copies of this notice including all test data. If you 

claimed any information as confidential, a single sanitized copy must also be submitted. 

Part II-HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

If there are several manufacture, processing, or use operations to be described in Part II, 

sections A and B of this notice, reproduce the sections as needed. 

Partiii-LISTOF ATTACHMENTS 

Attach additional sheets ifthere is not enough space to answer a question fully. Label each 

continuation sheet with the corresponding section heading. In Part Ill, list these 

attachments, any test data or other data and any optional information included in the notice. 

OPTIONAL INFORMATION 

You may include any information that you want EPA to consider in evaluating the new 

substance. On page II of this form, space has been provided for you to describe 

pollution prevention and recycling information you may have regarding the new substance. 

So-called "binding" boxes are included throughout this form for you to indicate your 

willingness to be bound to certain statements you make in this section, such as use, 

production volume, protective equipment . . . This option is intended to reduce delays that 

routinely accompany the development of consent orders or Significant New Use Rules. 

Except in the case of exemption applications (such as TMEA, L VE, LOREX) where 

certain information provided in such notification is binding on the submitter when the 

Agency approves the exemption application, checking a binding box in this notice does not 

by itself prohibit the submitter from later deviating from the information (except chemical 

identity) reported in the form. 

CONFIDENTIALITY CLAIMS 

You may claim any information in this notice as confidential. To assert a claim on the 

form, mark (X) the confidential box next to the information that you claim as confidentiaL 

To assert a claim in an attachment, circle or bracket the information you claim as 

confidential. If you claim information in the notices as confidential you must also provide 

a sanitized version of the notice (including attachments). For additional instructions on 

claiming information as confidential, read the Instructions Manual. 

Mark (x) if any information in this notice is claimed as confidentiaL 

EPA FORM 7710-25 (Rev. 5-95) 

TEST DATA AND OTHER DATA P-10-03 You are required to submit all test data in your possession or control and to 

provide a description of all other data known to or reasonably ascertainable by 

you, if these data are related to the health and environmental effects on the 

manufacture, processing, distribution in commerce, use, or disposal of the new 

chemical substance. Standard literature citations may be submitted for data in the 

open scientific literature. Complete test data (written in English) not summaries 

of data. must be submitted if they do not appear in the open literature. You should 

clearly identify whether test data is on the substance or on an analog. Also, the 

chemical composition of the tested material should be characterized. Following 

are examples oftest data and other data. Data should be submitted according to 

the requirements of §720.50 of the Premanufacture Notification Rule (40 CFR 

Part 720). 

Test Data (Check Below any included in this notice) 

Environmental fate data D Yes 

Health effects data D Yes 

Environmental effects data 0 Yes 

Physical/Chemical Properties* 0 Yes 

• Other data D Yes 

rusk assessments p 

Structure/activity r~]-ationships(KI 

Test data not in the-possession 

or control of the submitter 0 

A physical and chemical properties worksheet is located on the last page of this form. 1 .. _.1 

TYPE OF NOTICE (Check Only One) 

0 PMN (Prernanufacture Notice) 

D 

D 

D 

D 

D 

D 

INTERMEDIATE PMN (submitted in sequence with final produciPMN) 

SNUN (Significant New Use Notice) 

TMEA (Test Marketing Exemption Application) 

LYE (Low Volume Exemption)@ 40 CFR 723.50(c)(l) 

LOREX (Low Release/Low Exposure Exemption)@ 40 CFR 723.50(c)(2) 

L VE Modification 

IS THIS A CONSOLIDATED PMN? 

D 

D 

LOREX Modification 

Yes 
PMN Page 2 ; Page 2 of 40 

Public reporting burden for this collection of information is estimated to average I I 0 hours per response, includi~Rg~ ~b!Q~p~i~ 164 

instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of 

information. Send comments regarding the burden estimate or any other aspect of this collection of information, including suggestions for 

reducing this burden, to Director, Collection Strategies Division (2822), U.S. Environmental Protection Agency, 1200 Pennsylvania Ave., N.W., 

Washington, D.C. 20460; and to the Office of Management and Budget, Paperwork Reduction Act (2070-0012), Washington, D.C. 20503. 

CERTIFICATION -- A Printed copy of this signature page, with original signature, must be submitted 

I certifY that to the best of my knowledge and belief: 

I. The company named in Part I, section A, subsection la of this notice form intends to manufacture or import for a 

commercial purpose, other than in small quantities solely for research and development, the substance identified in Part I, 

Section B. 

2. All information provided in this notice is complete and truthful as ofthe date of submission. 

3. I am submitting with this notice all test data in my possession or control and a description of all other data known to or 

reasonably ascertainable by me as required by §720.50 of the Premanufacture Notification Rule. 

Additional Certification Statements: 

If you are submitting a PMN, Intermediate PMN, Consolidated PMN, or SNUN, check the following user fee certification 

statement that applies: 

D The Company named in Part I, Section A has remitted the fee of$2500 specified in 40 CFR 700.45(b), or 

D The Company named in Part I, Section A has remitted the fee of$1000 for an Intermediate PMN (defined@ 40 CFR 

700.43) in accordance with 40 CFR 700.45(b), or 

0 The Company named in Part I Section A is a small business concern under 40 CFR 700.43 and has remitted a fee of$100 

in accordance with 40 CFR 700.45(b). 

If you are submitting a low volume exemption (LVE) application in accordance with 40 CFR 723.50(c)(l) or a ),.ow release 

and low exposure exemption (LoRex) application in accordance with 40 CFR 723.50(c)(2), check the following certification 

statements: 

D The manufacturer submitting this notice intends to manufacture or import the new chemical substance for commercial 

purposes, other than in small quantities solely for research and development, under the terms of 40 CFR 723.50. 

0 The manufacturer is familiar with the terms of this section and will comply with those terms; and 

0 The new chemical substance for which the notice is submitted meets all applicable exemption conditions. 

D Ifthis application is for an LVE in accordance with 40 CFR 723.50(c)(l), the manufacturer intends to commence 

manufacture of the exempted substance for commercial purposes within I year of the date of the expiration of the 30 day 

review period.· 

The accuracy of the statements you make in this notice should reflect your best prediction of the anticipated facts regarding the chemical substance 

described herein. Any knowin!l and willful misinterpretation is subject to criminal penalty pursuant to 18 USC 1001. 

Signature and t~ of Anthony Official (Ori!!inal Signature Required) Prll.t 1-dl., "/- Date 

~/ 7/D. rCL -""/ -f! CEO /tJ -1- zoo? Signature~ (if applicable) Date 

EPA FORM 7710-25 (Rev. 5-95) Page 2 

Confidential 

D 

D 
Confidential 

Ia. Person 

Submitting 

Notice (in U.S.) 

--------------~-------------[] 

b. Agent (if 

applicable) 

f3ll¥h~~st NanoTechnologies, Inc. 

Mailing address. (number and street) 2501 Tecnnology Place 

City, State 

Norman, OK 

Name of authorized official 

Company 

Mailing address (number and street) 

City, State 

73071 

Postal Code Telephone (include area code) 

c. If you are submitting this notice as part of a joint submission, mark (X) this box·----------------------1~ 

Joint Submitter (if 

applicable) 

2. Technical 

Contact (in 

U.S.) 

Name of authorized official 

Company 

Mailing address (number and street) 

Province, Country 

Name. of authQTi;~:ed official PhilJ.p wallJ.s 

Comoanv . Soucnwest NanoTechnologJ.es, Inc. 

M ai I ing addressi( number1and street) 2501 -recrino ogy P ace 

City, State 

Norman, OK 

3. If you have had a prenotice communication (PC) concerning this notice and 

Code 

Postal Code 

73071 

EPA assigned a PC Number to the notice, enterth ::;:::,e.,::num=b~e::.,;r. ____ -+ 

4. 

5. 

6. 

If you previously submitted an exemption application for chemical 

substance covered by this notice, enter the exemption number assigned by 

EPA. If you previously submitted a PMN for this substance enter the PMN 

number EPA withdrawn or 

If you 

for the chemical substance covered by this notice, enter the notice number 

assigned by EPA. 

Type of Notice- Mark (X) I. 0 

D 

Manufacture 

Only 

Binding Option 

Mark 

EPA FORM 7710-25 (Rev. 5-95) 

X-09-0003 

I 2. 

I 

I 

I 

D 

D 

Page 3 

, State 

Telephone (include country or area code) 

acturing & Technical Support 

Telephone (include area code) 

405-217-8388 

Import 

Only 

Mark (X) 

if none 

Mark(X) 

if none 

Mark(X) 

if none 

Binding Option 

Mark 

I 

I 3. 

I 

I 

I 

D Both 

D[] 
PMN Page 4 ; Page 4 of 40 Page 000004 of 117 SAN 

Part I -- GENERAL INFORMATION-- Continued 

Section B --CHEMICAL IDENTITY INFORMATION: I You must provide a currently correct Chemical Abstracts (CA) name of the substance based on the ninth 

Collective Index (9Cl) ofCA nomenclature rules and conventions. 

Mark (X) the "Confidential" box next to any item you claim as confidential 

Complete either item I (Class I or 2 substances) or 2 (Polymers) as appropriate. Complete all other items. 

If another person will submit chemical identity information for you (for either Item I or 2), mark (X) the box at the right. D 

Identify the name, company, and address of that person in a continuation sheet. 

I. Class I or 2 chemical substances (for definition~ class I and class 2 substan~see the Instructions Manual) 

a. Class of substance - Mark (X) ~ Class I or I.,J Class 2 

b. Chemical name (Currently correct Chemical Abstracts (CA) Name that is consistent with TSCA Inventory listings for similar substances. For 

Class I substances a CA Index Name must be provided. For Class 2 substances either a CA Index Name orCA Preferred Name must be provided, 

which ever is appropriate based on CA 9CI nomenclature rules and conventions). 

Single-wall carbon nanotube 

c 

c. Please identify which method you used to develop or obtain the specified chemical identity information reported in this notice: (check one). 

D... Method I (CAS Inventory Expert Service- a copy of the Identification r;J Method 2 (Other Source) 

report obtamed from the CAS Inventory Expert Servtces must be t.::J 

submitted as an attachment to this notice) 

d. Molecular formula CBI 

D 

CAS Registry Number 

(if a number already 

exists for the substance) 

e. For a class I substance, provide a complete and correct chemical structure diagram. For a class 2 substance, provide a correct representative or 

partial chemical structure diagram, as complete as can be known, if one can be reasonably ascertained. Please see the E-PMN Instruction Manual for 

discussion of "native format" diagram software which can be helpful in reviewing your substance. 

SEE ATTACHMENT 10e STRUCTURE OF CARBONNANOTUBES 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page4 

Confidential 

D 

D 

D 

D 
Page 000005 of 117 SAN 

PMN Page 4a ; Page 5 of 40 

For a class 2 substance- (1) List the immediate precursor substances with their respective CAS Registry Numbers. (2) Describe the nature of the reaction or process. 

(3) Indicate the range of composition and the typical composition (where appropriate). 

e. (1) List the immediate precursor substances with their respective CAS Registry Numbers. 

Name (CAS#) 

e. (2) Describe the nature of the reaction or process. 

e. (3) Indicate the range of composition and the typical composition (where appropriate). 

0_ Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 4a 

Confidential 

D 

D 

D 
PMN Page 5 ; Page 6 of 40 Page 000006 of 117 SAN 

Part I-- GENERAL INFORMATION-- Continued 

Section B -- CHEMICAL IDENTITY INFORMATION-- Continued 

2. Polymers (For a definition of polymer, see the Instructions Manual.) Contidential 

a. Indicate the number-average weight of the lowest molecular weight composition of the polymer you intend to manufacture. D Indicate maximum weight percent oflow molecular weight species (not including residual monomers, reactants, or solvents) below 500 and 

below I ,000 absolute molecular weight of that composition. 

Describe the methods of measurement or the basis for your estimates: GPCU 

(i) lowest number average molecular weight: Oth~ r' (Spodfy bol~) 

I 

(ii) maximum weight% below 500 molecular weight: 

(iii) maximum weight % below I 000 molecular weight: 

0 Mark (X) this box if you attach a continuation sheet. 

b. You must make separate confidentiality claims for monomer or other reactant identity, composition information, and residual information. Mark (X) the 

"Confidential" box next to any item you claim as confidential 

(1) .. Provide the specific chemical name and CAS Registry Number (if a number exists) of each monomer or other reactant used in the manufacture of 

the polymer. 

(2) - Mark (X) this column if entry in column (1) is confidential. 

(3) - Indicate the typical weight percent of each monomer or other reactant in the polymer. 

(4) - Choose "yes" from drop down menu if you want a monomer or other reactant used at two weight percent or less to be listed as part of the polymer 

description on the TSCA Chemical Substance Inventory. 

(5) - Mark (X) this column if entries in columns (3) and (4) are· confidential. 

(6) - Indicate the maximum weight percent of each monomer or other reactant that may be present as a residual in the polymer as manufactured for 

commercial purposes. 

(7) - Mark (X) this column if entry in column (6) is confidential. 

Monomer or other reactant and CAS Registry Number Conti- Typical Include in Conti- Maximum Contidential composition identity dential residual dential 

(I) <n ill _ill_ (5) (6) (7) 

D % D D % D 

D % D D % D 

D % D D % D 

D % 

.... .. 11 D % D 

D % D % D 

D %1C D % D 

D % D D % D 

CJ %1 lc=J % . ···i 

CJ % [ lc=J % 

CJ % L lc=J % 

CJ % I ~lc=J % 

CJ % I Jc=J % I 

c=J % I ]c=J % I I 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 5 
c. 

d. 

e. 

PMN Page 5a; Page 7 of 40 Paae 000007 of 117 SAN 

P. !.ease identify which method you used to develop or obtain the specified chemical identityO.·.n .. £.ormation reported in this notice (check one). D Method I (CAS Inventory Expert Service- a copy of the identification report ... : Method 2 (other source) 

obtained from CAS Inventory Expert Service must be submitted as an attachment to this notice) 

The currently correct Chemical Abstracts (CA) name for the polymer that is consistent with TSCA Inventory listings for similar polymers. 

CAS Registry Number (if a number already exists for the substance) 

Provide a correct representative or partial chemical structure diagram, as complete as can be known, if one can be reasonably ascertained. . Please 

see the E-PMN Instruction Manual for discussion of "native format" diagram software which can be helpful in reviewing your substance. 

L ]Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page Sa 

CBI 

D 

D 

D 

D 
3. Impurities 

(a) - Identify each impurity that may be reasonably anticipated to be present in the chemical substance as manufactured for commercial purpose. Provide 

the CAS Registry Number if available. If there are unidentified impurities, enter "unidentified." 

Estimate the maximum % of each · If there are unidentified estimate their total %. 

Impurity and CAS Registry Number Maximum Confipercent dential 

Synonyms - Enter any chemical synonyms for the new chemical identified in subsection I or 2. 

None 

0 Mark (X) this box if you attach a continuation sheet. 

5. -List 

0 Mark (X) this box if you attach a continuation sheet. 

6. Generic chemical name- If you claim chemical identify as confidential, you must provide a generic name for your substance that reveals 

the specific chemical identity of the new chemical substance to the maximum extent possible. Refer to the 

TSCA Chemical Substance Inventory, 1985 Edition, Appendix B for guidance on developing generic names. 

% 

% 

% 

% 

% 

% 

new chemical substance. Provide the CAS 

CAS Number 

EPA FORM 7710-25 (Rev. 5-95) Page6 

w 

w 

w 

w 

D 

D 

D 

Confidential 

D 
PMN Page 7 ; Page 9 of 40 n. nnnnnn ,.r; ~ ~ "7 c A r-1 

Part I-- GENERAL INFORMATION-- Contmuea 

Section C --PRODUCTION, IMPORT, AND USE INFORMATION: 

Mark (X) the "Confidential" box next to any item you claim as confidential. 

1. Production volume-- Estimate the maximum production volume during the first 12 months of production. Also estimate the maximum 

production volume for any consecutive 12-month period during the first three years of production. Estimates should be on 100% new chemical 

substance basis. For a Low Volume Exemption application, if you choose to have your notice reviewed at a lower production volume than 

10,000 l<Jjyr, specify the volume and mark_(x) in the binding box. lfgranted,_you are bound to this volume 

Maximum first 12-month production (kg/yr) Maximum 12-month production (kg/yr) Confi-

(100% new chemical substance basis) (100% new chemical substance basis) dentlal 

Binding 

Option 

Mark (x) 

2. Use Information -- You must make separate confidentiality claims for the description of the category of use, the percent of production volume devoted to each 

category, the formulation of the new substance, and other use information. Mark (X) the "Confidential" Box next to any item you claim as confidential. 

a. (1) --Describe each intended category of use of the new chemical substance by function and application. (2) --Mark (X) this column if entry column (1) is 

confidential business information (CBI). (3) --Indicate your willingness to have the information provided in column (1) binding. (4) --Estimate the percent of total 

production for the first three years devoted to each category of use. (5) --Mark (X) this column if entry in column (4) is confidential business information (CBI). (6) --

Estimate the percent of the new substance as formulated in mixtures, suspensions, emulsions, solutions, or gels as manufactured for commercial purposes at sites under 

our control associated with each category of use. (7) --Mark (X) this column if entry in column (6) is confidential business information (CBI). (8) --Indicate% of 

product volume expected for the listed "use" sectors. Mark more than one box if appropriate. Mark (X) to indicate your willingness to have the use type provided in (8) 

binding. (9) --Mark (X) this column if entry(ies) in column (8) is (are) confidential business information (CBI). 

Category of use (I) (by function and application i.e. a CBI Binding Prod- CBI %in CBI %of substance expected per use CBI 

dispersive dye for finishing polyester fibers) ~~~n uc;,.on ~~~':: (8) 

Conductive coating using SWNT 

dispersion or SWNT ink. (see PMN pg 

10, pg lOa and Attachment) 

Additive(SWNT powder)in resins/thermoplastics/elastomers for mechanical 

reinforcement. (see Attachments) 

Manufacture of sheet composites 

(Buckypaper) using SWNT (no process 

information is available) 

(2) 

D 

D 

D 

D 

D 

D 

D 

(x) 

(3) 

D 

D 

D 

D 

D 

D 

D 

(4) 

% 

% 

% 

% 

% 

% 

% 

(5) (6) 

~ % 

~ % 

~ % 

D % 

D % 

D % 

D % 

(7) 

~ 

~ 

~ 

D 

D 

D 

D 

Site~ 

limited 

Consumer 

Industrial 

100 

100 

100 

Commercia! Binding 

Option 

D 

D 

D 

D 

D 

D 

D 

(9) 

D 

D 

D 

D 

D 

D 

D 

* If you have identified a "consumer" use, please provide on a continuation sheet a detailed description of the use(s) of this chemical substance in consumer products. 

In addition include estimates of the concentration of the new chemical substance as expected in consumer products and describe the chemical reactions by which this 

riubstance loses its identity in the consumer product. 

LJMark (X) this box if you attach a continuation sheet. 

b. Generic use If you claim any category of use description in subsection 2a as confidential, enter a generic description of that category. Read the Instruction 

description Manual for examples of generic use descriptions. 

Mark (X) this box if you attach a continuation sheet. 

3. Hazard Information -- Include in the notice a copy of reasonable facsimile of any hazard warning statement, label, material safety data sheet, or other 

information which will be provided to any person who is reasonably likely to be exposed to this substance regarding protective equipment or practices for 

the safe handing, transport, use, or disposal of the new substance. List in part III hazard information you include. 

Mark (X) this box if you attach hazard information. 

EPA FORM 7710-25 (Rev. 5-95) Page 7 

Binding 

Option 

Mark(x) 
Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER box next to any item you 

claim 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you 

control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if 

there are further industrial · or use after You must See manual 

of the site at which the will occur. 

Name 

SouthWest NanoTechnologies, Inc. 

Site address (number and street) 2501 Technology Place 

ZIP code 

Cleveland 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any ofthe sites have significantly different 

production rates or operations, include ali the information requested in this section for those 

sites 

I. Batch 

2. Continuous 

Maximum kg/day (100% new chemical Hours/day 

substance) 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

73071 

dential 

D 

D 

D 

(!) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon 

drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/day or kg/batch on a I 00% new chemical substance basis), and entry point of all starting materials and 

feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not 

used daily or per batch.). 

(3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media 

at the same step, assign a second release number for the second medium. 

attach a sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 8 
PMN Page Sa ; Page 11 of 40 Page 000011 of 117 SAN 

Confidential 

CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL 

EPA FORM 7710-25 (Rev. 5-95) Page 8a 
Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you 

control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if 

there are further industrial or use You must See manual 

of the site at which the will occur. 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different 

production rates or operations, include all the information requested in this section for those 

sites as 

1. Batch 

2. Continuous 

Hours/batch 

Maximum kg/day (100% new chemical Hours/day 

substance) 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

Use 

Batches/year 

Days/year 

dential 

D 

D 

D 

(I) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specif'y- e.g. 5 gallon pails, 55 gallon 

drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and 

feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not 

used daily or per batch.). 

(3) IdentifY by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media 

at the same step, assign a second release number for the second medium. 

attach a continuation sheet. 

EPAFORM7710-25 (Rev. 5-95) Page 8 -extra-1 
PMN Page Sa ; Page 13 of 40 Page 000013 of 117 SAN 

Confidential 

CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL 

EPA FORM 7710-25 (Rev. 5-95) Page 8a -extra-1 
box next to any item you 

claim 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you 

control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if 

there are further industrial or use after You must describe these · See instructions manual 

1. 

which the will occur. 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different 

production rates or operations, include all the information requested in this section for those 

sites as attachments. 

I. Batch 

2. Continuous 

Maximum kg/day (100%newchemical Hours/day 

substance) 

Use 

Batches/year 

Days/year 

Confidential 

D 

D 

D 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

(!) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon 

drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and 

feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not 

used daily or per batch.). 

(3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media 

at the same step, assign a second release number for the second medium. 

attach a continuation sheet. 

EPAFORM7710-25 (Rev. 5-95) Page 8 -extra-2 
PMN Page Sa ; Page 15 of 40 Page 000015 of 117 SAN 

Confidential 

CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL 

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-2 
Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER Mark (X) the "Confidential" box next to any item you 

claim as confidential 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you 

control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if 

there are or use after See 

1. 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different 

production rates or operations, include all the information requested in this section for those 

sites as attachments. 

I. Batch 

2. Continuous 

Maximum kg/day (100%newchemical Hours/day 

substance) 

Use 

Batches/year 

Confidential 

D 

D 

D 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

(I) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon 

drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and 

feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not 

used daily or per batch.). 

(3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media 

at the same step, assign a second release number for the second medium. 

attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 8 -extra-3 
PMN Page Sa ; Page 17 of 40 Page 000017 of 117 SAN 

Confidential 

CONFIDENTIAL CONFIDENTIAL . CONFIDENTIAL 

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-3 
Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER Mark (X) the "Confidential" box next to any item you 

claim as confidential 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you 

control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if 

there are after You must describe these See instructions manual 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different 

production rates or operations, include all the information requested in this section for those 

sites as 

Hours/batch 

I. Batch 

2. Continuous 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

dential 

D 

D 

D 

( 1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specifY- e.g. 5 gallon pails, 55 gallon 

drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and 

feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not 

used daily or per batch.). 

(3) Identity by number the points of release, including small or intermittent releases, to tbe environment of the new chemical substance. If releasing to two media 

at the same step, assign a second release number for the second medium. 

attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 8 -extra-4 
PMN Page Sa ; Page 19 of 40 Page 000019 of 117 SAN 

Confidential 

CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL 

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-4 
box next to any item you 

Complete section A for each type ofm<onnt~r·tm·<> 

control. Importers do not have to complete this 

there are further industrial 

operation involving the new chemical substance at industrial sites you 

outside the U.S.; however, you may still have reporting requirements if 

ou must describe these See instructions manual 

1. 

which the will occur. 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site, enter the number of sites. Identify the 

additional sites on a continuation sheet, and if any of the sites have significantly different 

production rates or operations, include all the information requested in this section for those 

sites as attachments. 

1. Batch 

2. Continuous 

Hours/batch 

Maximum kg/day (lOO%newchemical Hours/day 

substance) 

Use 

Batches/year 

Days/year 

Confi· 

dential 

D 

D 

D 

D 

d. Process description Mark (X) to indicate your willingness to have your process description binding. 

(I) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specif'y- e.g. 5 gallon pails, 55 gallon 

drum, rail car, tank truck, etc.). 

(2) Provide the identity , the approximate weight (by kg/day or kg/batch on a I 00% new chemical substance basis), and entry point of all'starting materials and 

feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not 

used daily or per batch.). 

(3) IdentifY by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media 

at the same step, assign a second release number for the second medium. 

attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 8 -extra-5 
PMN Page Sa ; Page 21 of 40 Page 000021 of 117 SAN 

Confidential 

CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL 

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-5 
Page 000022 of 117 SAN 

PMN Page 9 ; Page 22 of 40 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 

2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of 

works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. 

(2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. 

(5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the 

time of exposure. 

(7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). 

(8) --Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). 

(I 0) and (II) -- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

( 12) -- Mark (X) this column if entries in columns (I 0) and (II) are confidential business information {CBI). 

Worker activity CBI Protective Equipment/ Binding Physical Binding 

forms(s) 

CBI #of CBI Maximum duration CBI 

(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr 

Mark(x) substance Mark(x) Exposed 

(I) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) 

" D ~ " ~ 

" ~ " " ............... 

" D D ~ " ~ 

" D D ~ " ~ 

" D D ~ " ~ g D D ~ r--- g " g D D B " g -

D D D D D 

D D D D -

D 

D D D D 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9 
Page 000023 of 117 SAN 

PMN Page 9a; Page 23 of 40 

3. Environmental Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance 

released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3 ). 

(2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) --Mark (X) this column if entries in columns (I) and (2) are confidential business information (CBD. 

( 4) --IdentifY the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specifY)) to 

which the new substance will be released from that release point. 

(5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for 

each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the 

amount released to the environment after control technology (in kg/day). 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) --IdentifY the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBI). 

Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI 

Number substance released release efficiency data) 

(!) (2a) (2b) e.g. stack air Binding 

(3) (4) (Sa) Mark (Sb) (6) (X) 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

D See attached "Additional 

~ D Releases" for Releases 9 

through 11. 

(7) Mark (X) the destination(s) of releases to water. NPDES# CBI 

~ POTW--provide Non-contact cooling water to City of .Norman. OPDES 0029190 

name(s) D 

D Navigable waterway-- D provide name(s) 

D Other--SpecifY 

D 

] Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9a 
Page 000024 of 117 SAN 

PMN Page 9 ; Page 24 of 40 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 

2. Occupational Exposure --You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of 

works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(1) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. 

(2) --Mark (X) this column if entry in column (1) is confidential business information (CBI). 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. 

(5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the 

time of exposure. 

(7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). 

(8) --Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). 

( 1 0) and ( 11) -- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

_i12) -- Mark_fX) this column if entries in columns (10) and (11) are confidential business information (CBI). 

Worker activity CBI Protective Equipment/ Binding Physical Binding 

forms(s) 

CBI #of CBI Maximum duration CBI 

(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr 

Mark(x) substance Mark(x) Exposed 

(1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (II) (12) 

B D D B -

D 

B D B ....---- B " 8 D D B ....---- B " B -

D 8 ....---- B " 8 D D 8 -

8 " 8 D D B -

B " 8 D D 8 " - B 

8 D D B " " 8 D D B ....---- 8 " 8 D D B " 8 

1 Mark (X) this box if you attach a continuation sheet. 

EPA FORM 77!0-25 (Rev. 5-95) Page 9 -extra-1 
Page 000025 of 117 SAN 

PMN Page 9a ; Page 25 of 40 

3. Environmental Release and Disposal -- You must make separate confidentiality claims for the release number and the amount of the new chemical substance 

released and other release and disposal infonnation. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3). 

(2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) --Mark (X) this column if entries in columns (I) and (2) are confidential business infonnation (CBI). 

(4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to 

which the new substance will be released from that release point. 

(5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for 

each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the 

amount released to the environment after control technology (in kg/day). 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business infonnation (CBI). 

(7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business infonnation (CBI). 

Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI 

Number substance released release efficiency data) 

(I) (2a) (2b) e.g. stack air Binding 

(3) (4) (Sa) Mark (Sb) (6) _ffi 

D D D ~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

(7) Mark (X) the destination(s) 

D 

of releases to water. 

D 

NPDES# 

~ CBI 

1!1 POTW --provide City of Norman, OK OPDES 0029190 

name(s) D 

D Navigable waterway-- D provide name(s) 

D Other--Specify D 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-1 
Page 000026 of 117 SAN 

PMN Page 9 ; Page 26 of 40 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 

2. Occupational Exposure --You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of 

works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. 

(2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. 

(5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the 

time of exposure. 

(7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). 

(8) --Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). 

(I 0) and (II) -- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

(12) --Mark (X) this column if entries in columns (10) and (II) are confidential business information (CBI). 

Worker activity CBI Protective Equipment/ Binding Physical Binding 

forms(s) 

CBI #of CBI Maximum duration CBI 

(i.e., bag dumping, filling drums) Engineering Controls Option and %new Option Workers Hrs/day Days/yr 

Mark(x} substance Mark(x) Exposed 

(!) (2) (3) (4) (5) (6) (7) (8) (9) (!0) (ll) (12) 

~ D ~ D 

B D r----B r--- B " B D D ~ r--- B " D D ~ r--- 8 " " 8 D D ~ r--- 8 " 8 D D B -

8 " ~ D D B '---- " 8 ~ D D ~· r---

" " B D D B " 8 

D D D D D 

l Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-2 
Page 000027 of 117 SAN 

PMN Page 9a ; Page 27 of 40 

3. Environme11tal Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance 

released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3). 

(2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) --Mark (X) this column if entries in columns (I) and (2) are confidential business information (CBI). 

(4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to 

which the new substance will be released from that release point. 

(5) --a. Describe control technology, if any, and control efficiency that will be. used to limit the release of the new substance to the environment. For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for 

each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the 

amount released to the environment after control technology (in kg/day). 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBD. 

Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI 

Number substance released release efficiency data) 

(!) (2a) (2b) e.g. stack air Binding 

(4) Mark (3) (Sa) (X) (Sb) (6) 

~ D ~ 

~ D ~ 

~ D ~ 

D D D 

D D D 

D D D 

D D D 

D ' D D 

D D D 

(7) Mark (X) the destination(s) of releases to water. NPDES# CBI 

~ POTW --provide City of Norman, OK OPDES 0029190 

name(s) D 

D Navigable waterway-- D provide name( s) 

D Other--Specify D 

U Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-2 
Page 000028 of 117 SAN 

PMN Page 9 ; Page 28 of 40 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 

2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of 

works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. 

(2) --Mark (X) this column if entry in column (I) is confidential business information (CBD. 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. 

(5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the 

time of exposure. 

(7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). 

(8) --Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) --Mark (X) this column if entry in column (8) is confidential business information (CBD. 

(I 0) and ( 11) -- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

(12) --Mark (X) this column if entries in columns (10) and (11) are confidential business information {CBI). 

Worker activity CBI Protective Equipment/ Binding Physical Binding 

fonns(s) 

CBI #of CBI Maximum duration CBI 

(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr 

Mark(x) substance Mark(x) Exposed 

(1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) 

B D D B D 

B D -

B " " B D D B r-- B " B D D ~ r-- B " B D D ~ r--

" B 

D D D D -D 

D D D D - D 

D D D D r-- ;----

D D D D D 

D D D D D 

l Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-3 
Page 000029 of 117 SAN 

PMN Page 9a ; Page 29 of 40 

3. Environmental Release and Disposal -- You must make separate confidentiality claims for the release number and the amount of the new chemical substance 

released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3 ). 

(2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg' day or kg'batch). 

(3) --Mark (X) this column if entries in columns (I) and (2) are confidential business information (CBI). 

( 4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to 

which the new substance will be released from that release point. 

( 5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for 

each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the 

amount released to the environment after control technology (in kg'day). 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBI). 

Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI 

Number substance released release efficiency data) 

(I) (2a) (2b) e.g. stack air Binding 

(4) Mark (3) (5a) IX) (5b) (6) 

~ D ~ 

~ D ~ 

~ D ~ 

D D D 

D D D 

D D D 

D D D 

D D D 

D D D 

(7) Mark (X) the destination(s) of releases to water. NPDES# CBI 

~ POTW --provide City of Norman, OK OPDES 0029190 

name(s) D 

D Navigable waterway-- D provide name(s) 

D Other--Specify D 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-3 
Page 000030 of 117 SAN 

PMN Page 9 ; Page 30 of 40 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 

2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of 

works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. 

(2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. 

(5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the. 

time of exposure. 

(7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). 

(8) --Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). 

(10) and (II)-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

(12) --Mark (X) this column if entries in columns (10) and (II) are confidential business information (CBI). 

Worker activity CBI Protective Equipment/ Binding Physical Binding 

fonns(s) 

CBI #of CBI Maximum duration CBI 

(i.e., bag dumping, filling drums) Engineering Controls Option and o/onew Option Workers Hrs/day Days/yr Mark(x) substance Mark(x) Exposed 

(I) (2) (3) (4) (5) (6) (7) (8) (9) (10) (II) (12) 

B D D B D 

B D B -B " ~ 

B D D B ......-- B " B D D B ......-- 8 " 8 D D ~ ......--

" 8 

8 D D B -8 " 8 D D B " 8 

D D D D '----

D D D D ......-- D 

D D D D :--D 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-4 
Page 000031 of 117 SAN 

PMN Page 9a ; Page 31 of 40 

3. Environmental Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance 

released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

( 1) --Enter the number of each release point identified in the process description, part II, section A, subsection 1 d(3 ). 

(2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) --Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). 

( 4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to 

which the new substance will be released from that release point. 

(5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for 

each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the 

amount released to the environment after control technology (in kg/day). 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark(X) if the POTW name or NPDES #is confidential business information (CBI). 

Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI 

Number substance released release efficiency data) 

(!) (2a) (2b) e.g. stack air Binding 

(4) Mark (3) (Sa) (X) (Sb) (6) 

~ ~ ~ 

D D D 

D D D 

D D D .. D D D 

D D D 

D D D 

D D D 

D D D 

(7) Mark (X) the destination(s) of releases to water. NPDES# CBI 

~ POTW --provide City of Norman, OK OPDES 0029190 

name(s) D 

D Navigable waterway-- D provide name(s) 

D Other--Specify D 

] Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-4 
Page 000032 of 117 SAN 

PMN Page 9 ; Page 32 of 40 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 

2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of 

works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(!)--Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. 

(2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

( 4) and ( 6) -- Indicate your willingness to have the information provided in column (3) or ( 5) binding. 

(5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the 

time of exposure. 

(7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). 

(8) --Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). 

(10) and (II)-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

(12) --Mark (X) this column if entries in columns (10) and (II) are confidential business information (CBI). 

Worker activity CBI Protective Equipment/ Binding Physical Binding 

forms(s) 

CBI #of CBI Maximum duration CBI 

(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr 

Mark(x) substance Mark(x) Exposed 

(I) (2) (3) (4) (5) (6) (7) (8) (9) (10) (ll) (12) 

B D D B D 

8 D 8 ~ 

9 B 

B D D 8 ,.-B 9 

8 D D ~ ,.-

B 9 

B D D ~~ -B 

B D D 8 -

B 9 

B D D ~ 9 

- B 

B D D 8 9 " -

D D D D ,.-

D 

D D D D D 

D Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-5 
Page 000033 of 117 SAN 

PMN Page 9a ; Page 33 of 40 

3. Environmental Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance 

released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3 ). 

(2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) --Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). 

(4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to 

which the new substance will be released from that release point. 

(5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for 

each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the 

amount released to the environment after control technology (in kg/day). 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBI). 

Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI 

Number substance released release efficiency data) 

(1) (2a) (2b) e.g. stack air Binding 

(3) (4) . (Sa) Mark (Sb) (6) (X) 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

~ D ~ 

D D D 

D D D 

(7) Mark (X) the destination(s) of releases to water. NPDES# CBl 

[!] POTW--provide City of Norman, OK OPDES 0029190 

name(s) D 

0 Navigable waterway-- D provide name(s) 

D Other--Specify D 

J Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-5 
PMN Page 10; Page 34 of 40 Page 000034 of 117 SAN 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued 

Section B --INDUSTRIAL SITES CONTROLLED BY OTHERS 

Complete section B for typical processing or use operations involving the new chemical substance at sites you do not control. Importers do not have to complete this section for 

operations outside the U.S.; however, you must report any processing or use activities after import. See the Instructions Manual. Complete a separate section Bfor each type of 

processing, or use operation involving the new chemical substance. If the same operation is performed at more than one site describe the typical operation common to these 

sites. Identify additional sites on a continuation sheet. 

l(a). Operation Description-- To claim infonnation in this section as confidential, circle or bracket the specific infonnation that you claim as confidential. 

(I)-- Diagram the major unit operation steps and chemical conversions, including interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon drums, rail 

cars, tank trucks, etc). On the diagram, identify by letter and briefly describe each worker activity. (2) --Either in the diagram or in the text field !(b) below, provide the 

identity, the approximate weight (by kg/day or kg/batch, on an 100% new chemical substance basis), and entry point of all feedstocks (including reactants, solvents and 

catalysts, etc) and all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch). (3) --Either in the diagram or in the 

text field I (b) below, identify by number the points of release, including small or intermittent releases. to the environment of the new chemical substance. ( 4) Please enter 

CBI the# of sites (remember to identify the locations of these sites on a continuation sheet): --"1'-:---

# of sites 

CONFIDE}JTI.t\L Coi',Tf, IDE·~- TTT ~ T ..L ~ ..L "'J .L .J...L ~.L..J COl'~FIDENTIAL 

I (b). (Optional) This space is for a text description to clarify the diagram above. 

] Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page 10 
PMN Page 1 Oa ; Page 35 of 40 

2. Worker Exposure/Environmental Release Page 000035 of 117 SAN 

(I) --From the diagram above, provide the letter for each worker activity. Complete 2-8 for each worker activity described. 

(2) --Estimate the number of workers exposed for all sites combined. 

(4) --Estimate the typical duration of exposure per worker in (a) hours per day and (b) days per year. 

( 6) --Describe physical form of exposure and % new chemical substance (if in mixture), and any protective equipment and engineering controls, if any, used to protect 

workers. 

(7) --Estimate the percent of the new substance as formulated when packaged or used as a final product. 

(9) -- From the process diagram above, enter the number of each release point. Complete 9-13 for each release point identified. 

(10) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology to the environment (in kg/day or kg/batch). 

( 12) --Describe media of release i.e. stack air, fugitive air (optional-see Instructions Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify) 

and control technology, if any, that will be used to limit the release of the new substance to the environment. 

( 14) -- Identify byproducts which may result from the operation. 

(3), (5), (8 :(II), 13) and (!5) --Mark (X) this column if any of the proceeding entries are confidential business information (CBI). 

Lette #of CBI Duration Of CBI Protective Equip. /Engineering Controls/Physical Form and/% new substance %in CBI 

of Workers Exposure FormActi- Exposed ulation 

vity 

(1) (2) (3) (4a) (4b) (5) (6) (7) (8) 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

~ ~ ~ 

D D D Release Amount of CBI Media of Release & Control Technology CBI 

Number New 

Substance 

Released 

(9) _{lOa) lOb) 11 Iii\ (13) 

[g) [g) 

[g) [g) 

[g) [g) 

[g) [g) 

[g) [g) 

( 14) -- Byproducts: (15) 

D 

D Mark (X) this box if you attach a continuation sheet, 

OPTIONAL POLLUTION PREVENTION INFORMATION 

To claim information in the following section as confidential circle or bracket the specific information that you claim as confidential. 

In this section you may provide information not reported elsewhere in this form regarding your efforts to reduce or minimize potential risks 

associated with activities surrounding manufacturing, processing, use and disposal of the PMN substance. Please include new information 

pertinent to pollution prevention, including source reduction, recycling activities and safer processes or products available due to the new 

chemical substance. Source reduction includes the reduction in the amount or toxicity of chemical wastes by technological modification, 

process and procedure modification, product reformulation, raw materials substitution, and/or inventory control. Recycling refers to the 

reclamation of useful chemical components from wastes that would otherwise be treated or released as air emissions or water discharges, or 

land disposal. Descriptions of pollution prevention, source reduction and recycling should emphasize potential risk reduction subsequent to 

compliance with existing regulatory requirements and can be either quantitative or qualitative. The EPA is interested in the information to 

assess overall net reductions in toxicity or environmental releases and exposures, not the shifting of risks to other environmental media or nonenvironmental areas (e.g., occupational or consumer exposure). In addition, information on the relative cost or performance characteristics of 

the PMN substance to potential alternatives may be provided. 

All information provided in this section will be taken into consideration during the review of this substance. See PMN Instructions 

Manual and Pollution Prevention Guidance manual for e;uidance and examnles. 

EPA FORM 7710-25 (Rev. 5-95) Page lOa 
PMN Page 11 ; Page 36 of 40 Page 000036 of 117 SAN 

Optional Pollution Prevention Information (Continued) Describe the expected net benefits, such as (1) an overall reduction in risk to 

human health or the environment; (2) a reduction in the volume manufactured; (3) a reduction in the generation of waste materials 

through recycling, source reduction or other means; (4) a reduction in potential toxicity or human exposure and/or environmental 

release; (5) an increase in product performance, a decrease in the cost of production and/or improved operation efficiency of the new 

chemical substance in comparison to existing chemical substances used in similar application; or (6) the extent to which the new 

chemical substance may be a substitute for an existing substance that poses a greater overall risk to human health or the environment. 

CBI 

D 

[ ] Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev. 5-95) Page II 
PMN Page 12 ·Page 37 of 40 ' p a_g_e 000037 0 f 117 SAN 

Part III-- LIST OF ATTACHMENTS 

Attach continuation sheets for sections of the form and test data and other data (including physical/chemical properties and 

structure/activity information), and optional information after this page. Clearly identify the attachment and the section of the form to 

which it relates, if appropriate. Number consecutively the pages of any paper attachments. In the column below, enter the inclusive page 

numbers of each attachment. Electronic attachments can be identified by filename. 

Mark (X) the "Confidential" box next to any attachment name you claim as confidential. Read the Instructions Manual for guidance on 

how to claim any information in an attachment as confidential. You must include with the sanitized copy of the notice form a sanitized 

version of any attachment in which you claim information as confidential. 

# Attachment name Attachment Filename Attachment Confipage number( s) dential 

1 ~ 

2 ~ 

3 ~ 

4 10e Structure of Carbon Nanotubes 100-109 D 

5 SouthWest NanoTechnologies MSDS 110-114 D 

6 SouthWest NanoTechnologies Product Label 115 D 

7 ~ 

8 ~ 

9 ~ 10 ~ 

11 ~ 12 ~ 

13 ~ 14 ~ 15 ~ 

16 ~ 

17 ~ 

18 ~ 19 ~ 

20 ~ 

21 ~ 

22 Ohio EPA Hazardous Waste Facility Permit Renewal Letter 163-164 D 

23 D 

24 D 

[ ] Mark (X) this box if you attach a continuation sheet. Enter the attachment name and number. 

EPA FORM 7710-25 (Rev. 5-95) Page 12 
PMN Page 13 ; Page 38 of 40 p aae 000038 0 f 117 SAN 

PHYSICAL AND CHEMICAL PROPERTIES WORKSHEET 

To assist EPA's review of physical and chemical properties data, please complete the following worksheet for data you provide and 

include it in the notice. Identify the property measured, the page of the notice on which the property appears, the value of the property, 

the units in which the property is measured (as necessary), and whether or not the property is claimed as confidential. If the attachments 

are electronic, give the attachment number (found on page 12) at (b). The physical state of the neat substance should be provided. 

These measured properties should be for the neat (100% pure) chemical substance. Properties that are measured for mixtures or 

formulations should be so noted(% PMN substance in_). You are not required to submit this worksheet; however, EPA strongly 

recommends that you do so, as it will simplify review and ensure that confidential information is properly protected. You should submit 

this worksheet as a supplement to your submission of test data. This worksheet is not a substitute for submission of test data. 

Property Mark(X Page Value Measured o Confi~ 

if number Estimate dential 

(a) provided (b) (c) 

Mark(X) 

(MorE) (d) 

Physical state of neat substance D D . (s) D(l) Dcg) D 

Vapor pressure 

@Temperature oc D D Torr 

Density/relative density D glcm3 D 

Solubility 

D @ Temperature oc D 

Solvent giL 

Solubility in water {jiJ Temperature oc D giL D 

Melting teml'_erature D oc D 

Boiling I sublimation temperature(@ torr pressure D oc D 

Spectra D D 

Dissociation constant D D 

Particle size distribution D D 

Octanol I water partition coefficient D D 

Henry's Law constant D D 

Volatilization from water D D 

Volatilization from soil D D 

pH@ concentration D D 

Flammability D D 

Exl'_lodabili1y_ D D 

Adsorption I coefficient D D 

Other - Specify D D 

Other - Specify D D 

D Mark (X) this box if you attach a continuation sheet. Enter ihe attachment name and number. 

ATTACHMENT 

PAGE 13 
Page 000039 of 117 SAN 

Premanufacture Notice Electronic Continuation Sheet 

Continued From: Part II, Section A1d, pg 8 

Continuation Title: null 

Insert narrative continuation here Contidential 

D 

,, 

Insert any picture here (PDF) Contidential 

D 

D Check here to add another continuation sheet 

Page 39of 40 
Page 000040 of 117 SAN 

Premanufacture Notice Electronic Continuation Sheet 

Continued From: Part Ill, pg 12 

Continuation Title: List of Attachments 

Insert narrative continuation here Contidential 

D 

Insert any picture here (PDF) Contidential 

D 

D Check here to add another continuation sheet 

Page 40 of 40 
Page 000041 of 117 SAN 

CONSULTING LABORATORY 

Wastewater Treatability Report:· 

Impact to Wastewater Treatment Process 

Date of Report: August 15, 2008 

Report Prepared For: 

Joseph Bretz 

South West NanoTechnologies 

2501 Technology Place 

Norman, Oklahoma 73071 

Report Prepared By: 

RespirTek, Inc. 

James Radich 

Vice President of Research 

Laboratory Address: 

RespirTek, Inc. 

12450 Shortcut Rd. 

Bldg.F 

Biloxi, MS 39532 

Report Reviewed By: 

RespirTek, Inc. 

BJHook 

Technical Director 
Page 000042 of 117 SAN 

CONSULTING LABORATORY 

Final Report 

Date: 8/15/2008 

Prepared for: SouthWest Nano Technologies- Joseph Bretz 

Project ID: CHA-21 01 

Introduction 

The purpose of this project was to assess the impact of a low concentration 

discharge of a specific test compound provided by Southwest NanoTechnologies (SWeNT) relative to the on-site operations of Norman wastewater 

treatment facility (WWTF) in Norman, OK. SWeNT currently discharges to the 

WWTF, and prior to introducing new waste into the discharge stream, testing was 

conducted to evaluate the impacts to the WWTF. Analytical testing was 

conducted in conjunction with respiration monitoring as a means for quantifying 

the potential impacts. 

The Norman WWTF operates at an average influent rate of 10.5 MM GPO; 

however, a low-flow scenario was modeled to incorporate a worst-case basis for 

testing the additional waste component. The plant aeration basin operates 

between 5,400 and 6,400 mg/L total suspended solids (TSS). The discharge 

rate from SWeNT was provided by the project manager, and the test 

concentrations of the waste compound were agreed upon through discussions 

with the Norman pre-treatment coordinator prior to test initiation. 

Test Design and Setup 

Samples of the pre-treated waste material and standard discharge material from 

SWeNT arrived at the laboratory on 8 July 2008. Return activated sludge (RAS) 

and influent samples arrived on 9 July 2008. Two of the containers were outside 

of the recommended transport temperature of 2-soc (11.6°C and 16°C). 

Appropriate actions were taken, and the temperatures were noted in the event of 

unexpected test results. Four aerobic 2-L reactors were set up following receipt 

of all samples according to the reactor charge matrix presented in Table 1. 

Table 1. Reactor Material Charge 

Reactor ID Material Charge 

Rx 1 RAS +Influent 

Rx 2 RAS + Influent+ 0.001% Treatment 

Rx 3 RAS + Influent + 0.005% Treatment 

Rx 4 RAS + Influent + 0.023% Treatment 

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The influent was first decanted to remove additional solids present due to nonstandard operational conditions at the WWTP. RAS suspended solids 

concentration was 12,700 mg TSS/L upon arrival at the laboratory, and the 

appropriate volumes of RAS and influent were combined to model the WWTP's 

typical operating conditions in the aeration basin (-6,400 mg TSS/L). The test 

material supplied by SWeNT was prepared following guidance provided in an 

email from John Ferguson from Chasm on 8 July 2008. The goal was to model 

the discharge conditions from SWeNT and the treatment conditions at the WWTF 

as closely as possible such that the only deliberate differences would be the 

input of the new waste compound at the specific test concentrations. The 

percent solids of the material supplied by SWeNT was taken into account in test 

execution. 

After appropriate additions of materials were added to the preparatory flasks (4 

liters), respective volumes of the homogenized material were added to the 

reactors and time 0 sample containers. The reactors were then placed in a 

recirculating water bath at 20 °C and were allowed to equilibrate. Once ample 

time was given for temperature equilibration, the reactors were connected to the 

Challenge AER 200 Series Respirometer and aerobic respiration was monitored 

for 48 h. The reactors were stirred at -300 RPM to provide good saturation of 

oxygen to the aqueous phase and good mixing of the RAS and influent. 

Samples were collected from each reactor at the completion of aerobic 

monitoring, and the following parameters were analyzed: ammonia nitrogen 

(NH3-N), biochemical oxygen demand (BOD), chemical oxygen demand (COD), 

nitrite, nitrate, and TSS. All analytical samples were filtered prior to analysis to 

quantify aqueous concentrations of analytes. 

Results and Discussion 

Results indicate that the compound tested has no significant adverse affects on 

the respiration of the biomass in the WWTP. RX 1 had a cumulative oxygen 

uptake value of 168.22 mg 0 2/L; RX 2 uptake was 195.59 mg 02/L; RX 3 uptake 

was 165.805 mg 02/L; and RX 4 uptake was 173.915 mg 02/L. Only RX 2 

showed any significant difference in oxygen uptake in comparison to the baseline 

value with an increase of -28 mg 02/L. Respiration values for all other reactors 

were within standard deviation limits of quality control criteria for the 

respirometric equipment and are considered insignificant. In terms of biomass 

respiration and implied metabolic activity, no negative impacts were observed. 

The lowest concentration of the compound, RX 2 @ 0.001%, may assist in 

metabolism of wastewater as indicated by the significant increase in biomass 

respiration. 

Oxygen uptake rate values were relatively consistent amongst reactors with the 

exception of RX 3. The oxygen uptake rate for RX 3 shows some potential early 

inhibition (initial 12 hours) in relationship to the other reactors, but RX 3 had fully 

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Page 000044 of 117 SAN 

recovered by 20 hours and was respiring at higher rates than other reactors for 

the duration of the testing. Other reactors showed relatively little difference in 

respiration rates over the incubation period. This further indicates a lack of any 

significant impacts to biomass respiration by the introduction of the new waste 

compound. Cumulative oxygen uptake and oxygen uptake rate are provided in 

Figures 1 and 2 in the Data Appendices. 

Analytical results further indicate little, if any, significant impact to the wastewater 

treatment process. The waste compound exerts no observable differences in 

BOD values when time 0 BOD analytical results are compared amongst reactors. 

COD is slightly affected as indicated by a decrease in COD when compared 

amongst reactors. This phenomenon may be a result of some organic 

compounds within the wastewater adsorbing to the solid material tested. 

Nitrogen parameters (NH3-N, nitrate, nitrite) were unaffected by the addition of 

the test material to the reactors. All analytical results are provided in Table 1 in 

the Data Appendices. 

After incubation and treatment of reactor bottle contents by the active consortia, 

all analytical values with the exception of nitrate increased. Several reasons 

exist to explain this phenomenon, but the results are in line with previous testing 

conducted at RespirTek from Norman's \MJVTF. Ultimately, BOD and COD 

increases are likely a result of the inability of the analytical tests to accurately 

quantify initial (high) values. BOD and COD tests are only as reliable as the 

ability of the test to break down organic compounds (BOD- microbial, CODstrong oxidant). Given historical data obtained from Norman's \MJVTF and the 

consistency of the analytical test results, the observed increases are not 

laboratory error. 

Increases in NH3-N and nitrite indicate large molecules containing amino acids 

are broken down as treatment progresses and are partially nitrified, albeit slowly 

and to a low extent. Observed increases in TSS are a positive finding, as this 

indicates continued growth of biomass during metabolism of wastewater 

organics. Increases in biomass in respective reactors were as follows: RX 1 -

160 mg/L; RX 2 - 200 mg/L; RX 3 - 150 mg/L; and RX 4 - 120 mg/L. The 

increases are in line with other observations relative to treatability, particularly the 

increase in biomass relative to baseline in RX 2. 

Ending values for BOD show little difference between reactors. BOD results after 

incubation are as follows: RX 1 - 30 mg/L; RX 2 - 30 mg/L; RX 3 - 34 mg/L; and 

RX 4- 32 mg/L. The BOD results further indicate little, if any, impact to the 

treatment process by the addition of the test compound. Ending values for COD 

illustrate changes to the wastewater slightly more so than BOD. COD results 

after incubation are as follows: RX 1 - 115 mg/L; RX 2 - 112 mg/L; RX 3 - 119 

mg/L; and RX 4- 139 mg/L. With. the exception of RX 4, differences in ending 

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Page 000045 of 117 SAN 

COD values are not significant in relationship to test setup protocols and conduits 

for heterogeneous errors. 

Overall, significant impacts to the treatment process at the Norman VWVfF are 

not observed in this study. Some slight positive (RX 2 oxygen uptake) and slight 

negative (RX 4 TSS, COD) impacts were observed but not with urgent 

significance. The treatability of the new waste compound is not clear from the 

results of the testing. The only indication of treatability of the material is in RX 2 

in the form of increased respiration relative to baseline. Given the data obtained 

in the study, RespirTek concludes that no significant adverse impacts by the test 

compound were observed in the testing conducted. However, the actual fate of 

the test material is not clear but was not a specific goal of the study. 

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Data Appendices 

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Page 00004 7 of 117 SAN 

T a bl e 1 A na1y11ca I f I R esu It s S ummary 

RX 1 I RX2 I RX3 

Analytes (mg/L) Control I 0.001".4 treatment I 0.006% treatment 

'· 

Ammonia aN 9.88 115 9.64 16 9.39 18.8 

Carbonaceous BOD 12 30 11 30 11 . 34 

Chemical Oxygen Demand 82 116 49 112 68 119 

Nitrite 0.18 0.2 0.12 0.23 0.12 0.28 

Nitrate · ND ND ND ND ND ND 

Total Suspended Solids 15,220 6,380 15,240 15,440 15,280 15,430 

QA/QC Notes & Observations 

Holding Times: All samples analyzed within specified holding times 

Calibration: The instrument calibrations were within accepted criteria. 

Method Blanks: Method blanke were below MDL. 

Qualifiers: None 

,-------------·-"-" 

CumulativeOxygen Uptake 

~ 150 +-------------------------------~ .. ~----~-- .! 

J! 

.. 

~ ::;, J 100 +-----------------~~--~~~--~,ti~L-----------------------

10 15 20 25 30 35 40 45 50 

Tim a (h) 

Figure 1. Cumulative Oxygen Uptake 

RespirTek, Inc. 12450 Shortcut Rd., Bldg. F, Biloxi, MS 39532 228.392.7977 

I RX4 

I 0.023% treatment 

9.73 11.15 

12 32 

64 139 

ND 0.12 

ND ND 

15,230 15,3150 

-+-RX1- Control 

-a- RX2 • 0.001% Treatment 

· ·-'• ... RX3- 0.005% Treatment 

.......,_ RX 4 • 0.023% Treatment 

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www. respirtek.com 

I 

I 
Page 000048 of 117 SAN 

Oxygen Uptake Rate 

-t-RX 1· Control 

-11-RX2 • 0.001% Treatment 

RX 3 • 0.005% Treatment 

--;.+- RX 4- 0.023% Treatment 

10 15 20 25 30 35 40 45 50 

Time (h) 

Figure 2. Oxygen Uptake Rate 

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Page 000049 of 117 SAN 

ENSR AECOM 

Report of Short~ Term Chronic Toxicity Testing using the Water Flea 

(Ceriodaphnia dubia) 

Sponsor 

Project Officer 

Testing Facility 

Study Director 

Report Author 

Test 

Basis 

Project ID: 08503-132-265-005 

August2008 

Sponsor and Laboratory Information 

SouthWest Nano Technologies 

2501 Technology Place 

Norman, OK 73071 

John Ferguson {405) 217-8388 

ENSR 

Fort Collins Environmental Toxicology Laboratory 

4303 West LaPorte Ave. 

Fort Collins, CO 80521 

Fax: (970) 490-2963 

State of Florida NELAP Laboratory ID: E87972 

Rami B. Naddy, Ph.D. (970) 416-0916 ernail: maddy@ensr.aecom.com 

Gina McNerney (970) 416-0916 email: gmcnernev!alensr.aecom.com 

Test Information 

Short-Term Chronic under Static-Renewal Conditions 

USEPA (2002), method 1002.0 

Test Dates and Time August 15, 2008@ 1630 toAugust 22, 2008@ 1610 

Test Length 7 days 

Species Ceriodaphnia dubia 

Test Material Processed material (grab); Material with carbon nanotube 

(CNT}r:>_aste 

Dilution Water Moderately Hard Reconstituted Water 

Test Concentrations 0 (centro?, 0.5, 1, 5, 32% material, and 5% material+ CNT 

(0.023%) . . 

1 Target concentration was 0.5% material but do to an error, actual concentration was 5%; sufficient volume was 

prepared on day 0 and used throughout the study. The sample was stored at 4°C in the dark with no head space 

when not in use. CNT paste was added as received. 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page 1 
ENSR Page 000050 of 117 SAN 08503-132-265~005 

Material Collection and Receipt 

Note: See Appendix A for chain of custody records 

Material Characterization 

Initial Dilution/Control Water Characterization 

b Total residual chlorine 

c Measured in source water 

Test Conditions 

Type Static-Renewal Short-term Chronic 

Test Endpoints Survival and Reproduction 

Test Chambers 30-ml plastic cups 

Test Solution Volume 15 ml 

Replicates per Treatment 10 

Organisms per Replicate 1 

Test Temperature 25 ± 1°C 

Lighting Fluorescent, 16 hours light:8 hours dark 

Chamber Placement Random block, according to computer-generated chart 

Aeration None 

Test Solution Renewal Daily 

ENSR Fori Collins Environmental Toxicology Laboratory NELAC Accredited Page2 
ENSR Page 000051 of 117 SAN 08503-132-265-005 

Test Organism 

Species Ceriodaphnia dubia 

Age <24 hours (all within 8 hours) 

Source ENSR in house culture, batch 081408 

Acclimation Test temperature 

Feeding 0.2 ml YTC/Aigae mix per test chamber, daily during the 

test 

Reference Toxicant Testing Initiated August 19, 2008 using sodium chloride (NaCI) . 

TEST RESULTS 

Biological Data 

0 (Control) 100 100 100 90 90 90 90 N/A 23.1 N/A N/A 

0.5 100 100 100 100 100 100 100 N/A 22.3 No No 

1 100 100 90b 90 90 90 90 N/A 23.8 No No 

5 100 100 100 90 90 90 90 N/A 10.6 No Yes 

32 0 0 0 0 0 0 0 N/A 0.0 Yes 

5+CNT 100 100 100 100 100 80 80 N/A 7.4 No Yes 

Percent Minimum Significant Difference (Reproduction)c 50.1 Elevated 

Mean young per original female. any or higher were JJrv~•uc.;~::,u, 

mean young per female and statistical analysis of reproduction. 

bOne test organism was not found and was considered a mortality 

c PMSD value was determined using Dunnett's Test (a = 0.05) (Toxstat Version 3.5; WEST, Inc. and Gulley 1996). 

Note: See Appendix B for copies of laboratory data sheets 

ENSR Fort Colllns Environmental Toxicology Laboratory NELAC Accredited Page 3 
ENSR Page 000052 of 117 SAN 

Data Analysis 

Survival Significant Reduction Relative to the Control 

Normality8 

Homogeneity of Variance8 

Reproduction 

Significant Reduction Relative to the Control8 

Threshold Inhibition Concentration (IC25)b 

bUsing Norberg-King (1993) 

Test Endpoints 

Survival . NOEC 

LOEC 

NOEC 

ChV 

LOEC Reproduction 

NOEC = No Observed Effect Concentration 

LOEC = Lowest Observed Effect Concentration 

ChV = Chronic Value 

IC2s = 25% Inhibition Concentration 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

08503-132-265-005 

Inspection 

Shapiro-Wilk's Test 

Bartlett's Test 

Linear Interpolation 

:,;i!(ff{o:\M~~~~~·~·il:T:.::l:lW::·:::: 

5 

32 

1 

5 

2.2 

2.84 

Page4 
ENSR Page 000053 of 117 SAN 08503-132-265-005 

Physical and Chemical Data 

0 (Control) 8.0 8.3 6.3 7.1 273 298 24 25 

32 7.7 7.8 6.2 6.7 920 996 25 26 

24b 26b 

All Treatments 7.7 8.3 ;::5.98 NA 

24.0c 26.0c 

Performance NA Acceptable NA Acceptable 

saturation 

b Temperature In test solutions 

c Continuous wet temperature in the environmental chamber 

Reference Toxicant Test Results for C. dubia 

Procedural Deviations 

Temperature, pH, and dissolved oxygen were not measured in old solution from any test 

treatment on day 4. This procedural deviation likely had no impact on the outcome of this study. 

To the best of the Study Director's knowledge, no further deviations from the test procedure 

occurred during the study. 

References 

Norberg-King, T. 1993. A linear interpolation method for sublethal toxicity: the inhibition 

concentration (ICp) approach, version 2.0. National Effluent Toxicity Assessment Center, 

Technical Report 03-93. 

US EPA. 2002. Short-term methods for estimating the chronic toxicity of effluents and receiving 

waters to freshwater organisms. Fourth Edition. EPA-821-R-02-013. 

WEST, Inc. and D.O. Gulley. 1996. Toxstat Version 3.5. Western EcoSystems Technology, 

Inc., Cheyenne, WY. 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page 5 
ENSR Page 000054 of 117 SAN 

Statement of Quality Assurance 

The test data were reviewed by the Quality Assurance Unit to assure that the study was 

performed in accordance with the protocol and standard operating procedures, and that 

the resulting data and report meet the requirements of the NELAC standards. This 

report is an accurate reflection of the raw data. 

Quality ASSUanCeunit ~~ ~ t:t., woi 

Date 

Data Analyst 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page6 
ENSR Page 000055 of 117 SAN 

APPENDIX A 

Chain of Custody Records 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 
I 

ENSR I AECOM CHAIN OF CUSTODY RECORD 

I 

Client/Project Name: s L Project Location: pa--rlJ 

Analysis Requested 

Project Number: 'g("-o 3--l ?z- Reid Logbook No.: 

-:I''*t= o(i01t<-l 

Sampler {Print Name )/{Affiliation): Chain ofCustody Tape Nos.: 

"So\...... -C:\......'1:....., 

/! 2 Send Results/Report to: TAT: 

ct.M. ..... 

*-- 

.... -tr.e."-l). eo.. 

Fie( Sample 

c G Sample 0 R Field 

Date Time M A container Matrix Preserv. Filtered 

.... , L p 8 (Size/Mat'l) .I 

@ @ IX }(,L; 

nttL+O'l'tt l (J) IV--f , 

. 

Relinquished by: (Print Name)I(Affiliation) Date: .... t "'1- Received by: (Print Name)I(AIIilialionl Date: 7/J c-tB Analytical Laboratory 

"J¢\.._.J.. 0\'\- \.

<(')0 EN

Siqnature: :.....: \...-- Time: . .• 1. .4lD_:.J.c_. Time: 0150 

. 43

oal(?'..4" :Received by: (Print Name)I(Affiliationl Date: Fo

'6:5e. • _. .. 

»'co. }!_, Signature: Time: (9

Signatur¢ -

Date: Received by: (Print Name)I(Affiliation) Date: Sample Shipped Via

Si!lnature: Time: Sianature: lime: I FedEx Co

- . 

- .. . 

1'\.tm A- twGmt 1n n7Me ..,J,Lfl e UJOo Se
. 

=-:·:_·:···::; 

Respirtek, Inc. Laboratory 

12450 Shortcut Rd Suite F Biloxi, MS 39532 

Phone: 228-392-7977 Fax: 228-392-7838 

Email: bjhook@respirtek.com 

CHAIN-OF-CUSTODY RECORD (DOC PD-003) 

P.O. No. t T Project Name ANALYSES 

Samplers: 1. 1\ fu. 

(Signatures) 2. ----''\::__ ___ -_"1 _______ _ 

3. -------------------

Sample I.D. Date Time C G Coli No. of 

r By Cona 1 ,2,3 tainers 

b 

0\U V\ 1,?,1..., !Y"IJ·?rfi!\QI4 ·f !J

. \

1....-::: 

Re Relinquished by: Date J};) 

ReltJ.quislied by: -I Date Time Received by: Relinquished by: Date. Time Received t

Laboratory 

Transported by: DATE OF ANALYSIS: ANALYSIS TO BE COMPLE

REMARKS: ALL SAMPLES ICED IN THE _CALLOUT __ NO

FIELD AND DURING TRANSPORT TO THE RECEIVED: 

LAB. YES NO __ PRIORITY (NO CALLO

- - COMPLETED: UPON COMPLETION 

Original Copy - LAB Copy - FILE 
ENSR Page 000058 of 11 7 SAN 

APPENDIX B 

Test Data 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page B-1 
Page 000059 of 117 SAN 

Page 1 of __11_ 

FCETL QA Form No. 051 

Revision 4 

Effective 1/96 

TOXICITY DATA PACKAGE COVER SHEET ~ ~I¥J}otb 

~: !:nt0etlo't·Lor 

Test Type: Chronic Project Number: __,o...,.a ... s..._03,._-....l.1~32=---=26~5~-.... oo~s~-

Test Substance: Effluent Species: Ceriodaphnia dubia 

Concurrent Control Water Type: NA 

Organism Lot~mber: 0811./0& 

Age: £. 2.1./Jtij< 24 hr) Supplier: FCETL __.;......;;...;.;;;~----

Dilution Water Type: Mod Hard 

Date and Time Test Began: /kjo<l @ /~3 o j 

Protocol Number: USEPA 2002 + Reg. VI 

· Background Information 

pH control?: Yes ~ 

If yes, give % C02: 'iliA 

Env. Chm~. '*CD_T_e-st_C_h-mbrs: 30-ml cups 

Type of Test: Static~Renewal 

Test Temperature: 25 ± 1 oc 

Test Solution Vol.: 15 ml Number of Replicates per Treatment: 10 

Length ofTest: 3 broods Number of Organisms per Replicate: _____ 1~---

Type of Food and Quantity per Chamber: 0.2 ml YTC/ALG Feeding Frequency: 1 x Daily 

Test Substance Characterization Parameters and Frequency: 

Hardness: Sx Receipt Alkalinity: Sx Receipt NH3: Sx. Receipt TRC: Sx Receipt 

pH: Daily Conductivity: Daily and Test Termination* 

(i) 

. Test Concentrations (Volume:Volume): MH, 0.5, 1, 5, 32, and~ + CNT effluent 

Reference Toxicant Data: Test Dates: 

Hist. 95% Control Limits: to Method for Determining Ref. Tox. Value: 

Special Procedures and Considerations: 

•conductivity measured in dilution w~ter and 100% effluent at test termination 

D.O. maintained C!: 4.0 mg/L 

Study Director Initials: . '(lrZf--) Date: 

v/lr;/o~ . (9Cfuvl 011~105 € lcM:-e.. 
Project Number: 

MH 0 

0.5% 7 

1 

5% 72.5 

Page 000060 of 117 SAN 

TEST SUBSTANCE USAGE LOG 

08503-132~265-005 

1450 1450 

1443 1450 

436 1450 

1377.5 1450 

Page2ofj]_ 

FCETL QA Form No. 041 

Revision 4 

Effective 1/96 
I 

I 

I 

Cone. Day 

MH I 1 

2 

3 

4 

5 

6 

7 

8 

Total 

0.5% I 1 

2 

3 

4 

5 

6 

7 

8 

Total 

1% I 1 

2 

3 

4 

5 

6 

7 

8 

Total 

Day: 

Date: 

Time: 

Initials: 

Page 000061 of 117 SAN 

CERJODAPHNIA DUB/A 

CHRONIC BIOLOGICAL DATA 

Page3of 13 

FCETL QMorm No. 057 

Revision 1 

Effective 2/93 

Number of Neonates Produced and Survival of Original Organisms 

A B c D E F G H I J Total Mean Remarks 

0 0 0 0 0 0 0 0 0 0 qo1" 

tJ 0 0 a 0 0 0 0 0 0 

0 0 (3 0 '3/1 0 0 5/i Zf, 0 {b 

r?;, 0 0)( tJ 0 q, -t<;, 0 0 4-/, (4-

~.z l¥l 0 0 0 o/.2 JYz. 0 0 30 

P/:3 ~ IS/z 13/1 Gf/7.. OJ/t• 1-z.{::, lLI/3 0 ~, /2.. qi ,., ~U}d» I'\Jl6);>'-f'J 

·If 6 Y\1\.-n.P.DS 

5h '"'!, '?/2 1~/3 IY.:Y? ~ ~0 () I~/~ 13 ' (J) .. l /~/11 

z.r 1

stf D·--k to '"2-tf ~q. ~~ 30 '},. 4~ ~~.\1- I 13 1 I (?.S.'tp~) 

0 0 0 0 0 C) 0 0 0 0 JooLn 

0 0 0 0 0 0 0 0 c- 0 

0 0 0 0 I} I .:Jj, 0 0 2./' 0 Cp 

6?/i ;;<;, Lf/1 ~/, 0 () '+!, 71 'A.f bj, ;2.;1. ~ la~.c- r1eo 

IQ Q Q ~L OJfl. t-Y,_ '.Y!i 0 '9'::2. J'?'t.. _bS lJIItcurJ-v d~~~2t 

(2J 10{~ 10/2 16/:, 0 i1f13 ftf/3 () lqfs 1?/3 lO~ 

0 '<il ~ 'lb/3 0 o"~~ ~ 0 ·o'* 0 () '31 w. t.~elf'el neos-

. 

1 I '1:, '3 ~0 ]/ I '31..-' '2-t:{ I '3.1..- 3-'V 2~3 1-z..:~:>l 

0 0 0 0 0 0 0 0 0 0 '1o/1) 

I) 0 0 0 0 0 0 0 b 0 

0 0 <2> ();f)( (}·· ':t/t 0 1/l/ 3f, 3/, ~ tftt...rf= 

JtJ{ y, 6;, I tf; I 0 0 0 0 0 12. 

1)1•¥- )(~ rz. tYz ''¥'2. ~ 'Yz 0 lt?"2. 53 1ZM:f 1 Cti'Sj 'C- f'V.LQ 

'1/z ~~~(z. \ti/3 ~~/3 141/3 Nl/.z_ )IR/3 IU2 'fifj l\1 

0 'Vi/3 '?li-t ·o ~lt.l I~ C> n)'3 ~1-·;r ®'~_a.t 7 "Jts~c~ 

11--- ?:,~ 'Z..~· v-K 7.-Y,.. z,zJ '1.-<l '2.-~ ·?k 33.6 'LS..)f 1'2-1,1 

1 2 3 4 5 6 7 8 Key to symbols:f\lC=nor~ 

lfJ ... Ib'6, ~lt7/6l'J 8Jl~cA ~ '(, . .f~t· ~GI1J~ rfvfrA ~\~'tlti X- Original organism died. 

\Ottt1 It/Db lt'J?h Jo9( U2.b ltJS5 l1d~ M =Male I 

-~ IJ\e 2'i l..ho D'+ 157 fiM~ A .vcJ.oJt- '{t"'-!J,AtJ 

1-

1.$ 

1\fC 

lDM.-o~{og C @~~ eM\koC{u,(og e-f ·IV\#q 1 ::. 5/3 ~~~Attd lt{2.1 (off ef.; M I·H~ o &l?> ~ 

( 
II Project Number: 08503-132-265-005 

Page 000062 of 117 SAN 

CERIODAPHNIA DUB/A 

CHRONIC BIOLOGICAL DATA 

Page_ 4_of _fl 

FCETL QA Form No. 057 

Revision 1 

Effective 2/93 

Number of Neonates Produced and Survival of Original Organisms 

Cone. Day A B c D E F G H I J Total Mean Remarks 

I 5% I 1 0 0 0 0 0 0 0 0 0 0 q~7a 

2 0 0 0 0 0 0 0 0 0 0 

3 0 l/t :3ft ·0 0 0 0 ()) 0 0 tf 

4 ,Z1JI-: 0 0/ $j,K lf!\- 0 1~1 8jl i'-Y/ JC'!. 2..1 

5 'j/'2. ~ 1(9-'z. 1 0 0* 0 Q 0 G JS f>l'tv~U'~~ {!. 

6 G IIQ/~ fiR. :3 C9 0 0 ()) I 'l.. 0 33 

7 ~11'3 Cl/~ 'Ji.j 'It 0 0 "It 0 ~13 0 ~'3 '10 s~;vU '1)e.a:.:i 

8 

Total Jt:j lt-1• ~1' J;.-K 4 0~ ·~ "Z 'l 1.,.,- lb (, I)D, ~ I 

II 32% 1 o)( ()'/. ox O'f._ CJ'i.. {) "{ (') " 0'1. D.~ Q'{ '7"fl 

._ f,V 

2 

3 

4 

5 

6 

7 

8 I 

Total M 1)-'K. '0- ){ O-K D-\C o-k o--K D-X' tl..-JL O-K. v I Ov V I ~CNT 1 0 0 0 C) 0 0 0 0 0 0 <;(0 7~ 

5% (I) 2 0 0 0 0 0 0 0 0 0 0 

3 0 0 0 0 0 0 () Q) 0 Q 

4 ~I ~ v, \I\ tlj, t<,;, a;l 1;, \..), 1;, ltf 

5 o/'l., 'Yz_ n 0 0 0 0 (J 0 ~ Let 

6 0 0 &3 0>< 0 ~.Q. 0 (!) G)( (') lv 

7 \113 () 1J4 I .o ~t-:s 0 0 61<., :LS )6 .... ,.....,•frl)e--0 

8 l I 

Total ((p 5' \'-/_ .. kk'- 1L f<( 3 I J-K r; '9:LJ "t~J 

Day: 1 2 3 4 5 6 7 8 Key to symbols: N():..f\0~ 

Date: ~l111lbb ~nhb .g/1~01 ~/Jtilr. e.. 

~i ubs f/U/fi X = Original organism died. 

Time: m~ 1100 lk83 los::" UZ5 I~ }b JQ M =Male \ 

Initials: kc ~ OJ .Jo D~ ~ } Ctt~:U • ~)!) .1{\:k \,~ 

. C0hwn ~\~ .• ~ b 

II 

(), 6 

'- "'· 
Page 000063 of 117 SAN 

CHRONIC CHEMICAL DATA (INITIAL) 

Project Number: 08503-132-265-005 

Test Species: Ceriodaphnla dubia 

% Day Day Day Day Day Day 

1 2 3 4 5 6 

Cone.: IEJ 

IPH ~'I 

D.O. (mg/L} tR·1> 

Temp. COCl -z..5 

Cond. (IJS/cm) 1.-&\ (o 

Hard. (mglL) g{a qo 

Alk. _{mg/L) · (0_:.\ bl 

TRC (mg/L) 'ith<tl08 <0,{)2 o.ot.. 

NHa (mg/L) <\,o* 

Cone.: II 0.5% 

D.O. (mg/L) 

Temp. (°C) ~. ¥ -~· 

Cond. {J.l.S/cml 31 ( ~o5 -oo lS' 

Hard. (mg/L) 

Alk. (mg/L) 

TRC (mg/L) 

NH3 (mg/L) 

Cone.: 111.0% 

3 .. I 

D.O. (m__g/L} ~·l (0 /1 

Temp. eCl ~ ·'¥1 

Cond. (IJS/cml "2...'b7,. 2ll 

Cone.: II 5.0% I 

Initials: ~ .l:, ~ At<- JrK r2>Ff' Ak' 

Day 

7 

Page _5_ of J1 

FCETL QA Form No. 058 

Revision 3 

Effective 3/98 

~ 1lt }o'h 

QA-:, Mb'1 lo'1;lo~ 

Meter# Remarks 

All 

Cone. 

'" ~ 

037 

,;; 

1'nrl. 

·r\\YL 

fU) 

M·-1 

Note: Hardness, alkalinity, TRC, and NH3 data appearing on thls page have been transcribed from the wet chemistry log 

FCETL QA Form No. 084. . ~~O.Su.rtJ iV! SVU.Vc.t ~W *Dillutlon/control water and effluent were brought to 25•c prior to making the dlllution series. The temperature of resulting 

effluent dilution is assumed to also be 25"C. ~ Jrj( % [ {9lo '/, ~ 
Page 000064 of 117 SAN 

CHRONIC CHEMICAL DATA (INITIAL) 

Project Number: 08503-132-265-005 

Test Species: Cerlodaphnla dubia 

% 

cone.: 1132% I ~ 

[pH 7. C6 1\ t$ 

D.O. (mglL) (o.; U· b 

Con d. (IJS/cm) ~ 2,.0 ~U\\ 

Cone.: ~CNT 

Cone.: II 

[pH 

D.O. (mg/L} 

Cond. (IJS/cm) 

Cone.: U 

[pH 

D.O. (mg/L) 

Cond: (IJS!cm) 

Cone.: 11100% I 

IPH @ ·1.0 

D.O. (mg/L} -

Temp._CC_l 7.15' 

Cond. (J.JS/cm} G) \3 \~ 

Hard.lm_g/L) {:)9 {Q 7 2 

Alk. (mg/L) @ llf1 

TRC (mgliJ ® ..(0,02 

NH3 (mg/L) @.) ilf <._ \. 0 • 

Initials: }f ....bo --:li,. -~ !if lS# Prlt 

Page _a_ of \o 

FCETL QA Form No. 058 

Revision 3 

Effective 3/98 

~ tt/8/D'a 

tl)f+ l ~'l-lo<Hoi 

Day Meter# 

7 

All 

Cone. 

Remarks 

Note: Hardness, alkalinity, TRC, and~H3 data appearin~n this Gage have been tran~Cflbtd from the wet chemh\try log 

FCETL QA Form No. 084. £) W~ -, ~e~ 0~ 1o tf..t.tA.t~ 1 ~ \o'tJ M ~re~wt'olftU')piJ-. •oillu1ion/control water and effluent were brought to 25"C ·rio~ to a'K.ing the dillution series. The leTperature of resulting 

effluent dilution is assumed to also be 25"C. @ tt.J'V' q Q 10 5 6 fr>F\'~C ~lt9tb8 e 

@ fMv\ .fov-~? '~ls\o~ C-F)Nt\ Gr)Wl 'ljt?J o& 6 
I % 

Cone.: 

pH 

D.O. (mg/L) 

Temp (

0

C} 

Cone.: 

IPH 

D.O. (mg/L) 

Temp (°C) 

Cone.: 

pH 

D.O. (mg/L) 

Temp (°C} 

Cone.: 

IPH 

D.O. (mg/L) 

Temp (°C) 

Cone.: 

pH 

D.O. {mg/L) 

Temp (°C) 

Cone.: 

IPH 

D.O. (mg/L} 

Temp (°C) 

Cone.: 

IPH 

D.O. (mg/L) 

Temp (°C) 

\ 

Page 000065 of 117 SAN 

· Cerioda hnla dubia 

I Day Day Day Day Day Day Day Day 

1 2 3 4 5 6 7 8 

II MH I ( (iJ ~~.l&CJ 

/!.~ 8·8 ~~z..·· ·S. Q .. ~~0 ~·I ~1 

I#,· if '(1·3 (g.'-{ (;I rl-1 G.ii /ftq bJ3 

J<q As c9-4- 2 ilf !J-'7 I~ y- 'Z.t1 

II 0.5% I 

iJ,,f/ 'f1. ~ ~· r ~ 10 ~0 ~ \ \ ¥ .. 1 

ft;.'-/ ~ .. ;1.. /.a,:z. a z. 0.2 l/n. "1 e,~?. 

~0 ;)...'3 ~4 !ZJ 4, cP'f d4- ~lJl 

II 1% I 

~-~ lf.3 ~(2. 153'. ~I. ~~ ( ~,2 ~-- Q. 

{1,!./ &;.3 ·[a .. ( f:,, '3 G.~ (J;. ~ ;1'\~ 'l 

;l,{J? ~~ J>tt z -1 ~~ c9lf r'U·I 

II 5% I 

~· .!1 ~.9 ~·1. iS3, 8'. ( g,z t_. 1 

{l.d/ -.... 

~.' (p, { C,c- 6 ~{ (p.~ (,. b D 

tf{~ ~l/1 .al{-- 2) t ,.!)-L/ .:JL/ ~y 

II 32% I ¥qqc., 

apj.1.1 

(;;,t( ~ 

eR~ !$ ~~ 

~~·CNT 

5/.@ e,' (}.tl? ~~'2.. ~\. 8', l <t-2. ~~ '2_ 

(,.f 5~t:<J (;. 3 (;,, ) 

~ (J(/2 (p.CJ &~~ 

Jl& :2& ~~-~ I~ ~ B'-f BL{J "<.lf 

II I 

Date: ~/ir,/o& ~/17}(56 .51~ m~ WJ~ ~bolo~ eJttlaA! i\~<J~ 

Time: loQ) lc60 -~\R-0 Uc () ({QQ {;JJ:n U:>z.o 

Initials: JJ.c> --:Ji? 01 1) w w ~· Ct'l\·~ 

4 ~w? ~ 1'61~>~ w:r\· 

·,, 

Page _7 _of _1i · .. FCETL QA Form No. · ;g 

Revision 2 

Effective 1/96 / J ~ elf a o& a,fj-~M0<11>4 ~ 

Meter# Remarks 

All Cone. * conductivity 

"' conductivity 

* conductivity 

--)6NOIG ; PIY'H\,\ c.hunlst: 
Page 000066 of 117 SAN 

DAILY TOXICITY TEST LOG 

Project Number: 08503-132-265-005 

Test Species: Cerlodaphnla dubia 

General Neonates obtained from ~!'~!~~ !f~~(f-.tt.:~~erio monoculture board 

Comments (~ra-\v marked cups) 

Test Day 0 Test Solution Mixed at: ~~~S'" 

:est Organisms Added at: 1 ~:;o 

.. Test Day 1 CT~~ 2(c .. O 

Ra~= C(4l tt~c ;;,r;;.G •c. oc 

'1..- '2->.ti} . .- 2(,,. 0 °U 

Test Day 2 CT~°C Range~¥ tf d2G':&- oc . I ~ 

210--0 <(.... z.c.,. 0 ........ 2.(;,. 0 

Test Day 3 CT~°C Rang~· q., tf -;;x; . .iF 

@-z-5:.2... 1...- 'l.L{.(o- -z..rc:.. 0 °G 

Test Day 4 CT=o{~./p oc. Range= J?4. ~ e)-j. (c ac 

{f) 

Test Day 5 CT=2.lhb oc Range= ?.'·M_f- '2-Lf.(i, oc . 2 

Test Day 6 CT= f:}L\,?_°C Range= d) Lf( 0 -d y, ~c 

Test Day 7 CT= oc Range= oc @~,.~ ~~ ):;\-·~ s .e 

t..Y. .. O '2-4 ~ ()- 24 .1,; 

Test Day 8 CT= oc Range= oc 

~D.f-:J 'tf(UJ/Of}' E (O..W\ "'11.~1()~ w 

I 

Page _a_ of \& 

FCETL QA Form No. 055 

Revision 2 

Effective 1/94 _p / 

dlt; fJ?t(f}jlR /0'6 

Feeding Initials/Date 

0.2 ml YTC/ALG 

Daily 

Fed@ !~2-S" At> 

'tJif/6~ 

Fed @ro/fo ~ et 1c. bJe 

Fed@ f()t;'D + 

f3 Info~ 

Fed@ 

)(135 it 

Fed@ * IOfo f5/·LPJ/Ob 

Fed @~\05 J):.{J-

~/20/01; 

Fed@ n 

) ()'5 s w~t}ct 

Fed@ 

'1\ 0YH! ... r~r\\ 

~~'2.~/C6 
Random Board ID: H 

Project Number: 8503-132-265-005 

Replicate A B c D E 

Treatment 3 1 5 2 5 

# 

5 2 2 4 3 

2 4 6 3 2 

4 5 4 6 4 

1 3 1 5 1 

6 6 3 1 6 

1 = Control (Mod Hard) 

2= 0.5 3 = 1 

4= 5 

5= 32 

6 = 5+CNT 

0 k 

6 treatments X 1 0 re

- F G H 

4 3 5 

2 2 4 

1 1 3 

3 6 6 

5 4 2 

6 5 1 
Title: 

File: 

Page 000068 of 117 SAN 

8503-132-265-005 CC 8/15-22/08 repro/original 

265005R1.dat Transform: 

CJI!vutf~ tp(/:il 

Page_ffi_ot.ll_ 

Summary Statistics on Data 

NO TRANSFORMATION d 

afr"-. /tYW r; /z:J lo ~ TABLE 1 of 2 

GRP IDENTIFICATION 

1 

2 

3 

4 

5 

0 

0.5 

1 

5 

5' j)A''cNT 

N 

10 

10 

10 

10 

10 

MIN 

0.0000 

1. 0000 

0.0000 

0.0000 

1.0000 

MAX 

43.0000 

33.0000 

37.0000 

29.0000 

18.0000 

MEAN 

23.1000 

22.3000 

23.8000 

10.6000 

7.4000 

---------------------~-----------------------------------------------------

Summary Statistics on Data TABLE 2 of 2 

GRP IDENTIFICATION VARIANCE so SEM c.v. % 

1 0 189.8778 13.7796 4.3575 59.6520 

2 0.5 210.6778 14.5147 4.5900 65.0885 

3 1 113.2889 10.6437 3.3658 44.7215 

4 5 111.8222 10.5746 3.3440 99.7604 

5 5 ~CNT 48.7111 6.9793 2.2071 94.3153 

---------------11>----------------------------------------------------------

Title: 8503-132-265-005 CC 8/15-22/08 repro/original 

File: 265005Rl.dat Transform: 

D = 6069.4000 

w = 0.9438 

Shapiro - Wilk's Test for Normality 

Critical W = 0.9300 (alpha = 0.01 , N = 50) 

W 0.9470 (alpha = 0.05 , N = 50) 

Data PASS normality test (alpha = 0.01). Continue analysis. 

Title: 8503-132-265-005 CC 8/15-22/08 repro/original 

File: 265005Rl.dat Transform: 

Bartlett's Test for Homogeneity of Variance 

NO TRANSFORMATION 

NO TRANSFORMATION 

Calculated Bl statistic= 5.1038 (p-value = 0.2768) 

Data PASS Bl homogeneity test at 0.01 level. Continue analysis. 

Critical B 13.2767 (alpha 

9.4877 (alpha 

0.01, df 

0.05, df 

4) 

4) 
Page 000069 of 117 SAN 

Title: 8503-132-265-005 CC 8/15-22/08 repro/original 

File: 265005Rl.dat Transform: NO TRANSFORMATION 

SOURCE 

Between 

Within (Error) 

Total 

Critical F 

OF 

4 

45 

49 

ANOVA Table 

ss 

2436.9200 

6069.4000 

8506.3200 

3.7674 (alpha= 0.01, df = 4,45) 

2.5787 (alpha= 0.05, df = 4,45) 

MS 

609.2300 

134.8756 

F 

4 '5170 

(p-value = 0.0037) 

Since F > Critical F REJECT Ho: All equal (alpha 0.05) 

Title: 8503-132-265-005 CC B/15-22/08 repro/original 

File: 265005Rl.dat Transform: NO TRANSFORMATION 

Dunnett's Test TABLE 1 OF 2 Ho:Control<Treatment 

TRANSFORMED MEAN CALCULATED IN SIG 

GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05 

-------------------- ----------- ------------------ ------ 1 0 23.1000 23.1000 

2 0.5 22.3000 22.3000 0.1540 

3 1 23.8000 23.8000 -0.1348 

4 5 10.6000 10.6000 2.4067 * 

5 Q) S' 0 CNT 7. 4000 7.4000 3.0229 * ----------------------------------------------------------------------------

Dunnett critical value = 2.2300 (1 Tailed, alpha= 0.05, df [used] = 4,40) 

(Actual df = 4,45) 

Dunnett's Test TABLE 2 OF 2 Ho:Control<Treatment 

GROUP IDENTIFICATION 

NUM OF 

REPS 

1 0 10 

MIN SIG DIFF % OF DIFFERENCE 

(IN ORIG. UNITS) CONTROL FROM CONTROL 

2 0.5 10 11.5821 50.1 0.8000 

3 1 10 11.5821 50.1 -0.7000 

4 5 10 11.5821 50.1 12.5000 

--~----------~-~-~-=~~----~~-----------==~=~:=-------=~~~------==~~~~~--
Page 000070 of 117 SAN 

08503-132-265-005 

Cone. ID 1 2 3 4 5 

Cone. Tested 0 0.5 1 5 32 

Response 1 21 2 12 19 0 

Response 2 39 33 37 27 0 

Response 3 0 30 27 29 0 

Response 4 20 31 0 5 0 

Response 5 24 1 25 4 0 

Response 6 27 32 22 0 0 

Response 7 25 29 28 9 0 

Response 8 30 1 28 3 0 

Response 9 2 32 26 8 0 

Response 10 43 32 33 2 0 

--------------------------------------------------------------

*** Inhibition Concentration Percentage Estimate *** 

Toxicant/Effluent: effluent 

Test Start Date: 8/15/08 Test Ending Date: 8/22/08 

Test Species: Ceriodaphnia 

Test Duration: 7 days 

DATA FILE: 265005R.icp 

OUTPUT FILE: 265005R.i25 

Cone. Number Concentration Response Std. Pooled 

ID Replicates % Means Dev. Response Means 

----------------------------------------------------------------------- 1 10 0.000 23.100 13.780 23.100 

2 10 0.500 22.300 14.515 23.050 

3 10 1.000 23.800 10.644 23.050 

4 10 5.000 10.600 10.575 10.600 

5 10 32.000 0.000 0.000 0.000 

The Linear Interpolation Estimate: 2.8394 Entered P Value: 25 

Number of Resamplings: 80 

The Bootstrap Estimates Mean: 

Original Confidence Limits: 

Resampling time in Seconds: 

2.5011 Standard Deviation: 1.2735 

Lower: 0.4866 Upper: 5.3125 

0.00 Random_Seed: -366960776 
Page 000071 of 117 SAN Page:~ If 1]. 

FCETL QA Form No. 15 

Effective: 5/90 

~ lt r/-o 8 SUBJECT: DAILY LOG 

ALL ENTRIES MUST BE INITIALLED WITH DATE AND TIME: 

" r;),, 

Pr-eP 1"2-L. t5t th'S . tf)l £ ~~ Uo~ .... 1 

• ~} v>tl( ~ (..(lp\)1{ ~ ..Po.r 'td n:F ~ T#!Pype -fiv1 ~ ~j~~...,~'3/t) 

~DO M. 1 .:F' i;;J>P[IJ:tiy1 ~-.r:: f'; !l~ .f7J Jr:::L- ILJ$tt4..l 

M--b.l tlvJ r ILL.)# ~~~v I . ~ • a..W. 0 0'1... "J '7.-a <5' w <:..iS l ~~~ 1P fT:l< !r:f 

{r:tF 2-z.ovi./l 

./ 

D,o2~ 7. t4: {.), 0'25q ..(',;:. i{' o .a:£:1.6\ X:.o, 

tOI} ..... I ;00 M J - J 7.-GQI ,.,.., 

1IG = "2.,% 4 

._ A .. !..diAl ... "j{ ~ ~v+l:, l S' A 

-r J 

• . m ""' i -1 ul.,... . .~. t.a-;.:;J l.CPl, '"·~tv1 ;vi ~[A .${A.Ji411 /o...;.U~ 

,.,J,L 60 M-1 ,.,'od~·t .....A r~ ~z...(... ~ tA.A,I)} 

· Ill. .5" f<!,..J. Tf d.<. p.,., ... , . 'A ,) I 

/. , l'rvrlt' AJ...LJIA~ (JJ;tj 5-z ·~DTt\ 

lia ~ 'Jq/Kt u 

V' F 
Page 000072 of 117 SAN 

ENSR AECOM 

Report of Short· Term Chronic Toxicity Testing using the Fathead Minnow 

(Pimephales promelas) 

Sponsor 

Project Officer 

Testing Facility 

Study Director 

Report Author 

Test 

Basis 

Project ID: 08503-132-265-006 

August 2008 

Sponsor and Laboratory Information 

SouthWest Nano Technologies 

2505 Technology Plaqe 

Norman, Oklahoma 73071 

John Ferguson (415) 217-8388 

ENSR 

Fort Collins Environmental Toxicology Laboratory 

4303 West LaPorte Ave. 

Fort Collins, C0.80521 

Fax: (970) 490-2963 

State of Florida NELAP Laboratory ID: E87972 

Rami B. Naddy, Ph.D. (970) 416-0916 email: maddy@ensr.aecom.com 

Rami Naddy {970) 416-0916 

Test Information 

Short-Term Chronic under Static-Renewal Conditions 

USEPA (2002), method 1000.0 

Test Dates and Time August 15, 2008 @ 1630 to August 22, 2008 @ 1550 .. 

Test Length 7 days 

Species Pimephales promelas 

Test Material Processed Material (grab}; Material with carbon nanotube 

{CNT) paste 

Dilution Water Moderately Hard Reconstituted Water 

Test Concentrations 0 (control}, 0.5, 1, 5, 32% Material and 5% Material + CNT 

{0.023%)' 

1 Target concentration was 0.5% material but do to an error, actual concentration was 5%; sufficient 

volume was prepared on day 0 and used throughout the study. The sample was stored at 4°C in the dark 

with no head space when not in use. CNT paste was added as received. 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page 1 
ENSR Page 000073 of 117 SAN 08503-132-265-006 

Material Collection and Receipt 

Material Characterization 

Initial Dilution/Control Water Characterization 

b Total residual chlorine 

c Measured In source water 

Test Conditions 

Type Static-Renewal Short-term Chronic 

Test Endpoints Survival and Growth {Dry Weight Per Original Fish) 

Test Chambers 500-ml plastic cups 

Test Solution Volume 250ml 

Replicates per Treatment 5 

Organisms per Replicate 8 

Test Temperature 25 ± 1°C 

Lighting Fluorescent, 16 hours light:S.hours dark 

Chamber Placement Random according to computer-generated chart 

Aeration Initiated on day 3 because of low DO in 5% + CNT 

treatment; ta[get of 100 bubbles per minute 

Test Solution Renewal Daily 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page2 
ENSR Page 000074 of 117 SAN 08503-132-265-006 

Test Organism 

Species Pimephales promelas 

Age <24 hours 

Source ENSR in house culture, batch 081508 

Acclimation Test temperature 

Feeding 0.1 ml brine shrimp nauplii per test chamber 3x/day 

durina the test 

Reference Toxicant Testing Initiated August 8, 2008 using sodium chloride (NaCI) 

TEST RESULTS 

Biological Data 

0 (Control} 100 100 100 100 100 100b 0.586 N/A N/A 

0.5 100 100 100 100 97.1c 97.1 97.1 0.571 No No 

1 100 97.5 97.5 97.5 97.5 97.5 95 0.501 No No 

5 100 100 100 100 97.1c,d 97.1 97.1 0.504 No No 

32 0 0.000 Yes 

5+CNT 100 100 100 100 100 100 100 0.546 No No 

Percent Minimum Significant Difference (Growth) 6 18.0 Acceptable 

based on the original number of organisms per replicate. 

b Test organisms in replicate B were excluded from analysis because aeration was too vigorous in this treatment, 

which lead to Increased mortality; 3 organisms in replicate D were also excluded from analysis due to a similar 

problem. This did not appear to be a problem in other replicates or treatments. 

cOne organism was accidentally killed during the test due to a technician error and excluded from statistical analysis. 

dOne test organism was not found and was considered a mortality. 

e PMSD value was determined using Bonferroni t-Test (a= 0.05) (Toxstat Version 3.5; WEST, Inc. and Gulley 1996). 

Note: See Appendix B for copies of laboratory data sheets. 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page3 

----------------------------~~----
ENSR 

Survival 

Growth 

(per original fish) 

Page 000075 of 117 SAN 

Data Analysis 

Normality 

Homogeneity of Variance 

niflcant Reduction Relative to the 

Control 

Normality a 

Homogeneity of Variance a 

Threshold Inhibition Concentration (IC25)b 

(WEST, Inc. and Gulley 1996) 

bUsing Norberg-King (1993) 

Survival 

Growth 

(per original fish) 

NOEC = No Observed Effect Concentration 

LOEC = Lowest Observed Effect Concentration 

ChV = Chronic Value 

IC25 = 25% Inhibition Concentration 

Test Endpoints 

08503-132-265-006 

N/A 

N/A 

Inspection 

Shapiro-Wilk's Test (a=0.01) 

Bartlett's Test (a=0.01) 

Linear Interpolation 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page4 
ENSR Page 000076 of 117 SAN 08503-132-265-006 

Physical and Chemical Data 

0 (Control) 7.8 8.2 5.8 7.1 273 301 24 26 

32 7.8 7.9 6.4 6.7 641 948 25 26 

24b 26b 

All Treatments 7.6 8.3 ::::3.7a NA 

24.0° 26.0° 

Performance NA Acceptable NA Acceptable 

DO of 

b Temperature in test solutions 

c Continuous wet temperature in the environmental chamber 

Reference Toxicant Test Results for P. promelas 

Procedural Deviations 

The bubbling rate during aeration to increase the dissolved oxygen was observed to be too high 

on days 6 and 7 because of an equipment malfunction. This was the likely cause of the 

observed mortalities of the organisms in the control treatment. Because the observed 

mortalities in the control treatments were believed to be caused by excessive air flow, these 

organisms were excluded from statistical analyses. To the best of the Study Director's 

·knowledge, no further deviations from standard test procedures occurred during the study. 

References 

Norberg-King, T. 1993. A linear interpolation method for sublethal toxicity: the inhibition 

concentration (ICp) approach, version 2.0. National Effluent Toxicity Assessment Center, 

Technical Report 03-93. 

US EPA. 2002. Short-term methods for estimating the chronic toxicity of effluents and receiving 

waters to freshwater organisms. Fourth Edition. EPA-821-R-02-013. 

WEST, Inc. and D.O. Gulley. 1996. Toxstat Version 3.5. Western EcoSystems Technology, 

Inc., Cheyenne, WY. 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page 5 
ENSR Page 000077 of 117 SAN 08503-132-265-006 

Statement of Quality Assurance 

The test data were reviewed by the Quality Assurance Unit to assure that the study was 

performed in accordance with the protocol and standard operating procedures, .and that 

the resulting data and report meet the requirements of the NELAC standards. This 

report is an accurate reflection of the raw data. 

Quality Assurance Unit Date 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page6 
ENSR Page 000078 of 117 SAN 08503-132-265-006 

APPENDIX A 

Chain of Custody Records 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page A-1 
! 

ENSR I AECOM CHAIN OF CUSTODY RECORD 

l 

Client/Project Name: s l. Je.. Project Location: pcf--ru 

Analysis Requested 

Project Number: ?z- 2{? Field Logbook No.: 

. 

Sampler (Print Name)I(Affilialion): Chain of CUstody Tape Nos.: 

""J'.s\..,... - 6,.,, .. ... \..,J-t7-: '!D"f f-'=f (rr.!r&d-) 

... -?;it /1 JI- 

Send Resu!ts/Report to: TAT: 

... -u.e. ...... 

.... ea.. '"" 

Fie(Sample NoJidentificati: c G Sample 8 0 R Reid 

Date. lime M A Container Matrix PreseN. Rltered 

)._ p ..B (Size/Mat'l) J 

e#tl.)e/)L )< 

f 

Relinquished by: (Print Name)I(Afliliation) Date: Received by: (Print Name)I(Afliliation) Date: if1 Analytical Laboratory 

-:Jd\..._.._ on 

Signature: \...--- Time: "( IW.,. Signature:liJ •.... Time: OlSO EN

. 43

. Received by: (Print Name)I(Affiliation) Date: Fo

es$e. • 'i!£"'7'.,? .... · 

Signatuw, U>. Signature: · Time: (9

Date: Received by: (Print Name)I(Afliiation) Date: 

Sample Shipped Via

Signature: Time: Sil:mature: Time: FedEx Co

R.Coli'ts 1'0 07.doc Se
Respirtek, Inc. 

12450 Shortcut Rd Suite F Biloxi, MS 39532 

Phone: 228--392· 7977 Fax: 7838 

Email: bjhook@respirtek.com 

:': . 

:_ :· .. . 

. : .. 

CHAIN-OF-CUSTODY RECORD QlOC PD-003) 

P.O. No. 

Samplers: 1. 

(Signatures) 2. 

3. 

Sample I.D. I Date I Time c I G con I No.of 

t.J\ L I Y'llb[... 

l ah!AJA I 16 

Rel{rlquisb.ed by; -, Date I Time 

REMARKS: ALL SAMPLES ICED IN THE 

FIELD AND DURING TRANSPORT TO THE 

LAB. YES NO 

'copy - LAB Copy- FILE 

r By Cona 1,2,3 tainers 

b 

Received by: Received by: 

Transported by: 

ANALYSES 

Relinquished by: 

Relinquished by: 

DATE OF ANALYSIS: 

RECEIVED: 

COMPLETED: 

Date I Time'· 

1/li) 

Date I Time 

·f 

Received 

Laborato

ANALYSIS TO BE COMPL

__ CALLOUT __ N

__ PRIORITY (NO CALLO

UPON COMPLETION 
ENSR Page 000081 of 117 SAN 

APPENDIXB 

Test Data 

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

08503-132-265-006 

Page B-1 
Page 000082 of 117 SAN 

Page 1 of ~~ FCETL QA Form No. 051 

Revision 4 

Effective 1/96 

TOXICITY DATA PACKAGE COVER SHEET (§rrv/!JilJ?J9/ oi;/of' 

Test Type: Chronic Project Number: 08503-132-265-006 

Test Substance: Effluent Species: Plmephale$ prometas 

Dilution Water Type: .....;.;.M;.:o~d..:..H;.:a:;.;rd=------- Organism Lot ~umber: 08[}/J l6 

Concurrent Control Water Type: -...;..N;;.;./A...;.._ __ _ Age: "'-2 t..i hr:t< 24 hr) Supplier: FCETL 

~;;...;;;..;~----

Date and Time Test Began: ghsz'p~ @ /~'J;,D 

Protocol Number: USEPA 2002 + Reg. VI 

Background Information 

pH control?: Yes @ 

If yes, give % C02 Type ofTest: Static-Renewal : 

Test Temperature: 25 ± 1 oc Env. Chmb@:~(p Test Chmbrs:~~~~~e!<!ia<!.!lk~er!..2s 

Test Solution Vol.: 250m! Number of Replicates per Treatment: ___ ....;5=-----

Length of Test: 7days Number of Organisms per Replicate: ___ ...;8;;._ __ _ 

Type of Food and Quantity per Chamber: 0.10 ml B.S. Feeding Frequency: 3 x Daily 

Test Substance Characterization Parameters and Frequency: 

Hardness: Sx Receipt Alkalinity: Sx Receipt NHa: Sx Receipt TRC: Sx Receipt 

pH: Daily Conductivity: Daily 

s-1-J' 

Test Concentrations (Volume:Volume): MH, 0.5, ·1. ·5, 32% and ¢o with CNT Effluent 

Agency Summary Sheet(s)?: ..;..N;;;..A.;__ ______ _ 

Reference Toxicant Data: Test D~tes: tJf rf o;; to ¥'flr'/OX 

Hlst. 95% Control Limits: -=tfl1 to Method for Determining Ref. Tox. Value: Linear Interpolation 

Special Procedures and Considerations: 

D.O. maintained<: 4.0 mg/L 

•conductivity measured in dilution water and 100% effluent at test termination 

If survival In any test chamber falls below 50%, reduce feeding in that chamber to 0.05 ml of brine shrimp 

I 

J J . 

Study Director Initials: f'l-~ Date: ?f(rr(D? 

® g (c; .. o.q <:t=' {1j)UJJ ?/1//)r e f...,f· "!-1 \v~ 
Project Number: 

0.5% 7 

1% 15 

73 

Page 000083 of 117 SAN 

TEST SUBSTANCE USAGE LOG 

08503-132-265-006 

Page 2 of .Jt_ 

FCETL QA Form No. 041 

Revision 4 

Effective 1/96 
IIProject Number· 

Test· 

%Cone. Replicate 

MH A 

8 

c 

D 

E 

0.5% A 

B 

c 

D 

E 

1% A 

B 

c 

D 

E 

5% A 

B 

c 

D 

E 

32% A 

B 

c 

D 

~·CNT A 

:YJ 8 

c 

D 

E 

Date: 

Time: 

Initials: 

Page 000084 of 117 SAN 

FATHEAD MINNOW (PIMEPHALES PROMELAS) 

CHRONIC BIOLOGICAL DATA 

Page 3 of lf_ 

FCETL QA Form No. 060 

Revision 2 

Effective 1/96 

08503-132-265-006 II 

Day 

0 

Day 

1 

·8 

8 

6 

8 

r o 

f 0 

r o 

Day 

2 

8 

B 

8 

g 

8 

8 

8 

Day 

3 

8 

·1-

-~-

Number of Surviving Organisms 

Day Day Day Day 

4 5 6 7 Remarks 

_S I? If"' at.!J "~ ..Cc~ N. .t.la.., {trt,J :v.y 

s ~) ~ <Z 

·'It 1 re. 

n 111n -

Jro 7-b 
Page 000085 of 117 SAN 

CHRONIC CHEMICAL DATA (INITIAL) 

Project Number: 08503-132-265-006 

Test Species: Plmephales promelas 

o/o Day Day Day Day Day Day 

1 2 3 4 5 6 

Cone.: 

B. I h. I sf. I gv~ ~l 1{1 ~~-~ lt-~ (._5 ::;} l~cS 6~l 

"1?5 ~5 1-Cj '1--S 12S v~ . Cond. (IJS/em) -zqfo IJ<'q~ t?<.t1B 1.-9? 1_91.,. 2qt.l 1_,-:f.-; 

Hard. (mg/L) ~to qo 

Alk. (mg/L) ~t./ fot 

TRC (mg/L) t.O .1>1/ o·oz. 

•. -. NHa (mg/L) L, J .. o~ ~\.OP 

Cone.: II 0.5% I 

[p_H tl e3 ·' Brl g .l g_ '2.. g", I 8.) 

D.O. (mg/L) fo. i tJ[j (f.fJ , __ , 7.1 Cell-f 7_1 

JO. ~ "t 7¥: ~ -4-

Cond. (IJS/em) "3( I ~05 t!;06 '7u; '?t.l"L 2-11 '2.~ ·~ 

Hard. (mg/L) 

Alk. (mg/L} 

TRC (mg/L) 

NH3 (mg/L) 

Cone.: II 1 o/o I 

IPH 

D.O. (mg/L) l!. 1 

Cond. {IJS/cm) l9/ 

Cone.: II 5% I 

[pH 

D.O. (mg/L) 

Initials: ltf icb? kA, hk flK IJ(yf PI( 

Day 

7 

...... .cn'J 

Page 4 of /f( FCETL QA Form No. 058 

Revision 3 

Effective 3/98 

Meter# Remarks 

All 

Cone. 

f~o 

~ 

P31 

I~ 

1-r\fr 

'l;fr 

'Zo ' I 

l~t' I t.r (\'..e.,.s v.W ~,.. 

.S'b\\fl.'-' w..t 

Note: Hardness, alkalinity, TRC and NH3 data appearing on this page have been transcribed from the wet chemistry log 

FCETL QA Form No. 084. 

*Dillutionfcontrol water and effluent were brought to 25"C prior to making the dillution series. The temperature of resulting 

effluent dilution_Jf as~u~~d to also be 25"C. 

~'b~~~ 

'""' 
Page 000086 of 117 SAN 

CHRONIC CHEMICAL DATA (INITIAL) 

Project Number: 08503-132-265-006 

Test Species: Plmephales promelas 

% I Day Day Day Day Day Day Day 

0 1 2 3 4 5 6 

Cone.: 

11 32% I 

IPH 7.1> 1\CJ. W11. 

D.O. (mg/L) &·7 (~'v ~ 

Temp. (°C) 1\ lfr\ ~ 

Con d. (I.JS/em} _./}_ ~z.o ftJ iff\ 

Cone.: l~!oCNT 

IPH '.:?) .,.~ '1.~ 1·~ :t.}) 7-.$ 7.R 'f:g 

D.O. (mg/L} /c() fDJ1 -h-7- "6vG 1\ I t,.(;, G._-g 

Temp. (°C) 'Vi ~B cf<B ~) .z...~ 2.5 t)/§ 

Cond. (J.IS/em) ~'l'l> 1Lt15' 406 11191 14-&~ 402- 'i9tJ 

Cone.: II I 

iPH 

D.O._{_mg/IJ 

Temp. (°C} 

Cond. (IJS/cm) 

Cone.: II 

pH 

D.O. (mg/L) 

Temp. (°C) 

Cond. (J.JS/cm) : 

Cone.: ll100% I 

IPH ~ 11,0 

D.O. (mg/L) - - Temp. (°C) z~ 'Z\ -zr- -z.:) ~< "2-s- '"1..~-

Cond. (I.JS/cm} ® 1'31.5 

Hard. (mg/L) ~ ltJ1J. 

Alk. (mg/Ll 5 14·7 

TRC (mg/L} @ .c.o.O'Z. 

INH3 (mg/L) <\.0' 

Date: lltrfog I~ II" Jo ~ (J)/7/0'f3 81/K!tJP. 'ltt9/o& ·;,.d 7f 18Z/7a5 Uuft,9 

Time: ~11:>- !{;13~ 104Ci HOS lo'lA wss to S-o 

Initials: llfJ ~ I~ /)vf'/. Jyf( -Off 7JI 

Day 

7 

Page 5of/K 

FCETL QA Form No. 058 

Revision 3 

Effective 3/98 

Meter# Remarks 

All 

Cone. 

Note. Hardness, alkalinity, TRC, and NH3 data appeanng on th1s page have been transcnbed from the wet chem1stry log 

FCETL QA Form No. 084. 

*Dill uti on/control water and effluent were brought to 25"C prior to making the dillutlon series. The temperature of resulting 

effluent dilytlop is assumed to also be 25•c. /::;;:-.. ~1. J c- ~~illhlog' \Z)~ 1;/tfl(Gr ~ ~ b l Qr{(J'O ·V-1' @IN! ~'ilo'PioSJ tJI~ri.~J1.dJJ 

A~71~oK lfo-~ ~ 
% Day Day 

1 2 

Cone.: II 1\JIU II IV" 0 

IPH 8., I ?J·O 

D.O._(mg/L) ((;.'3 (p.O 

Temp {

0

C} a to ~" Cone.: II o.5% 

"[pH e.o 1.'1 

D.O. (mg/L) w·O s.t./-

Temp (°C) lb?& p,.fp 

Cone.: II 1% I 

.f:>H 9./ /.y 

D.O. (mg/L) {p·O ~.Lf 

Temp (

0

C) RCt> 6!" 

Cone.:- II 5% I 

pH H. I '1· t.} 

D.O. (mg/L) ~.3 ~0 

Temp (°C_l 6?0 ~lo 

Cone.: II 32% 1~"148 . !pH '1.1 

D.O. (mg/Lj &;.t.f 

Temp {

0

C) c:'2 ib?& ~(J) 

Cone.: ~/oCNT 

[pH (!:) &.0 7ff' 

D.O. (mg/L) (p./1{ s·.o 

Temp (°C) !cR&L ~~ 

Cone.: II I 

[pH 

D.O.(mg/Lj 

Temp (°C) 

Date: ~I&Ael3 &ln/ol 

Time: ~s llJS-o 

Initials: dKJ c:¥ 

Page 000087 of 117 SAN 

Day Day Day Day Day 

3 4 5 6 7 

* :,o\ t ,)!, . 1·'0 1~e Z. I ~·0 

.. '1 ~fJ (1), t..l ~t3 {:;. to~ I 

z.,s ()r::; 2.L.( ~Cf ~ ,, 

1.& '7.1 <$v2. ~-' SJ. 7.. 

lf--S &..6 ~t3 6.!; lAZ 

'1.--~ &s I~L( 1-lf c94 

1-;, ~ f1.o 1At2.. -~-' '1.1 

/lv; II.Jl. 5 ~3 ~c 6'~ G'.3 

'1,5_ _a~ 2W 1/4 ~5 

54& 

1.1 f1.o 9>~-, 13. { w.,z ii~O I&.S 15~~ rtf (o"() 

1.-S _t?7f3 L.L.J _U cDs 

* 

*~q.q 

1-.~ ~. l 1p7 q_,_~ ~.1 

iv~· C;.tf 'J/J q(~ ~' q 

1-9 ~6 t2.L{ 9).f ~11 

$/13~8 lJh~l~ d!A-t.~tl. Q!~~ ~hl/.91 '0/t~ 

~lOS tf.O@ UiS~ Lliltf-0 /S3() 

}J( Jtl .w Ak ~ 

Day 

8 

Page6 of J!. 

FCETL QA Form No. 059 

Revision 2 

Effective 1/96 

Meter# Remarks 

All Cone. * conductivity 

lfo 

5 

·03]_ 

* conductivity 

*conductivity 

CD ~flx·A-~-0 '~- ({D~ '1/t (j)X 1...-/.) 
Page 000088 of 117 SAN 

DAILY TOXICITY TEST LOG 

Project Number: 08503-132-265~006 

Test Species: Plmephales promelas 

General 

Comments 

Test Day 0 Test Solution Mixed at: /b/'i" 

Test Organisms Added at: /h-:;tl 

Test Day 1 CT=~{!) oc 

'l£..o Range =·z;;.t."'\•w.o 1P oc 

~S.3··'Zk·O 

Test Day 2 CT=~I>\-rip<D oc Range=~O oc 

~.o 9(9,o -~ID·O 

Test Day 3 cT~W oc Range=,tt.~ oc 

'1- 9 .. & ~ · ~U:.h - -;,--G.o 

J4.r ~5~{,_.; ~~t~eve_ t.fOO~ 

Test Day 4 CT= :l£f"--b oc u Range = '}.. y, "L .... 1.Jh' oc 

. Test Day 5 0 CT= ],lfvb oc Range = ~Cf-- ~ .- 1,- cf_ (; oc 

(})1.-

Test Day6 ®o~J:toc Range= Z/1:vO "~€) oc 

v-l· '2..- 3 

Test Day 7 CT= oc Range = :?'I. 0 _ EN.9. oc 

~tf.O 

a.U)at~ .fw kt?~ ~"' J..,-w, .c.~\u.r.f 

Test Day 8 CT= 0 

oc Range= oc 

(Va(08\\1'\08 £:... w (T.u'V\ q_ ,,, 0~ & 

00 

Page 7 of )f 

FCETL QA Form No. 055 

Revision 2 

Effective 1/94 

crt"': MW~-fot:t I o& 

Feeding initials/Date 

0.10 ml B.S. 

3X Daily 

Fed@17PWt. ~ 

~<5/dl 

Fed @ o 'f{)OCI\) oO 

l:21o E-s ~f,bf 6.9 t~o(')lo) 

Fed @o14ocw 

l.;l.CO~ C'.IJ 

1715/Jf/. <ah~o& 

Fed @ ogt.j.o &s 1/·fr- ~$-to ,4r' 

~wcyt ~2/i~$ 

Fed @oeovc~'h. \;215- D JK 

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llk 

lfPO c:g: $/ztf&.j 

NONE l>?/Rs 

~)zz_~~ 
5E 6A 

4E 1E 

2A 4C 

5A 3E 

1 =Control 

SIX TREATMENTS FIVE REPLICATES 

RANDOM CHAMBER LOCATION "Tau" 

Study #8503-132-265-006 

2E 60 50 20 

1C 1A 48 18 

6E 6C 58 2C 

28 3A 68 10 

2 = 0.5 

3 = 1 %..efftttafit ® 

4=5 

5 = 32 

6 s-2 . 

(f) 

Q),.L?J ? It /D '%" GP @1)0_ -fiK. C/1) 01[-o & (Sf.. 

30 

3C 

38 

40 
(( 

fuv-plL.-.. TEST ORGANISM LENGTHS. WEIGHTS. AND LOADING 

- ' 

ProjectNumber: . Test Substance: (!) 

Species: -. - lj)_ ""'"'"'-'4.. \ Analyst <{:' ,-.Jt 

-

Analyst Gross: Af 

Date/Time Wt.: { 2 2. ( 0 & @ i 3 "16 Datemme of Gross Wt.: q 1'1-J{ o$ 8.. \l3o 

Boat Treatment Rep. length WeightTyp: (Circle): ·wet Blot Drv Or; AFDW (>SOO"C) 

No. Units: 

Tare Gross Net Weight Adjusted No. of MeanWlper 

Weight (g) Weight (g) {g) Net Weight Orig. Original 

(g)1 ' Organisms Organism 

. 6) 

(mg) 

fVLK _A:-

1Z:. o .nstg 0 ( J11S> rrd.\at .___. -

- c_ o. o.Cf-;lS) 0-6oCJ07 

'P o.q31% o. o.063oo 56 >TEs1 . 

f;. o.13b&6 o.l}y II'/ () -01>1.f31./ 

(J.t;'o/ .. A- o. Cf3ZIIJ I o. \ 

1> 0.()05"1-b 

-

C-- 6· Do'{ 11 

l> o."':;lo-:; o.oo1-i1 . -, [4. T€) 

E:. Q.9YIOI 

l fo il+ o. q3 . o.o03J6 

I'K 0·00%1 

Blank 

Range 

Mean 

Test Solution Volume: Loading Rate: 

A -1..1 :-...... -!.-.1...& 1.- • L ._ .. 

(l ftf '\b.J{-.'tl v 

'" ) 

Comments: 

Analytical Balance 10: S1r1l- 

Dried in from Dat

to Dat

LotorQum

-- 

MeanWlper No. of 

Treatment Surv. 

{mg) Organisms 

{Original) 

q 

0 

g 

s 

'1> 

cg 

co 

' b 

tf)-%--7 

'i 

. ' 

j 
h fwpll.. 

-· 

Proiect Number: · -l'52 z.i. S' ... 

Species: -. _ ?. 

. . . 

Date/Time oftare Wt: l 2.2--{ \) & @ \ 31.( o 

TEST ORGANISM LENGTHS. WEIGHTS. AND LOADING 

- .. 

Test Substance: M-&>11 Comments: 

Analyst Tare: £1'1\'\\ 

/ Analytical Balance 10: 

Analyst Gross: Af Dried in from Oa

Date/TimeofGrossWt: @. /\36 

to 

Boat Treatment Rep. length WeiahtType (Circle): ·wet Blot Dry AFDW (>500"C) Lot triitch'1u

No. Units: 

Tare Gross 

Weight (g) Weight (g) 

. 

c.. 0 ().ct15i3 

1) 

l.o.9sl5b o.ot4n1 

A-

<g 

c.. (). 1; 

(). <i:SI.f tll O.'l>?>S i -. 

_../') E 

o/!L.r:.;.rr A- '() .. 

ri> 1> Oo'131s1 

c.. fi.Ot!>qlbt. 

'P o.t:t.sz o.'t;ln 

Q 

ttf:mk. e.: OeCJ$b 72 

Range 

Mean 

Test Solution Volume: 

A.....l...l =- 1-.-.- -.£ Ll 1- L- .1. '!'..!!: .. / 

li>(/ 

Net Weight uusmcr _.x-No.of MeanWtper MeanWtper 

{g) Orig. Original Treatment 

(9)1 . Organisms Organism (rng) 

(mg) (Original) 

0 .Ont.f lt. 

0 .bbl!Z-3 

b.oo:,;n e) 

o.oeLfiS"" 

O.Oo?,-u, 

b.OD3'1Df. 

6.{)0\Nt:, 

o.oo4n 

Loading Rate: 

"---""' 

No. of 

Surv. 

Organisms 

1 

<6 

?> 

b 
TEST ORGANISM LENGTHS, WEIGHTS, AND LOADING 

Project Number: 8503-132-265-006 

Length Tare Gross Net Weight 

Treatment Rep Units: Weight {g) Weight (g) (g) 

A 0.93393 0.93848 0.00455 

B "l 0.00000 MH c - 0.93248 0.93755 0.00507 

D 0.93146 0.93446 0.00300 

E 0.93680 0.94114 0.00434 

A 0.93219 0.93717 0.00498 

B 0.93048 0.93574 0.00526 

0.5% c 0.93249 0.93666 0.00417 

D 0.93352 0.93703 0.00351 

E 0.93657 0.94101 0.00444 

A 0.93084 0.93420 0.00336 

B 0.92946 0.93433 0.00487 

1% c 0.93167 0.93583 0.00416 

D 0.93317 0.93660 0.00343 

E 0.93750 0.94173 0.00423 

A 0.93285 0.93645 0.00360 

B 0.93138 0.93475 0.00337 

5% c 0.93421 0.93896 0.00475 

D 0.93414 0.93881 0.00467 

E 0.93143 0.93471 0.00328 

'"4 

A 0.93594 0.93993 0.00399 

B 0.93737 0.94179 0.00442 

c 0.93536 0.93982 0.00446 

® D 0.93296 0.93716 0.00420 E 0.93672 0.94149 0.00477 

(f) -·f I 

@ ,..6.rJ /t>e e 

Species: Fathead minnow 

Mean We/ Mean 'Wt.J 

Adjusted Original Treatment Number of 

Net Weight No ofOrig. Organism (mg) Surv. 

(g) Organisms (mg) (OriginaO Organisms 

0.00455 8 0.569 0.5862 8 

0.00000 0 -- 0 

0.00507 8 0.634 8 

0.00300 5 0.600 5 

0.00434 8 0.543 8 

0.00498 8 0.622 0.5715 8 

0.00526 8 0.658 8 

0.00417 8 0.521 8 

0.00351 7 0.501 6 0.00444 8 0.555 8 

0.00336 8 0.420 0.5013 7 

0.00487 8 0.609 8 

0.00416 8 0.520 8 

0.00343 8 0.429 7 

0.00423 8 0.529 8 

0.00360 8 0.450 0.5038 8 

0.00337 7 0.481 6 

0.00475 8 . 0.594 8 0.00467 8 0.584 8 

0.00328 8 ' 0.410 8 

0.00399 8 0.499 0.5460 8 

0.00442 8 0.552 8 0.00446 8 0.558 8 

0.00420 8 0.525 8 

0.00477 8 0.596 8 

y- . 

. e-t::h, I'M 

v-u<>_) 
TEST ORGANISM LENGTHS, WEIGHTS, AND LOADING 

Project Number. 8503-132-265-006 Species: Fathead minnow 

Mean Wr.J Mean Wr.J 

Adjusted Original Treatment 

Length Tare Gross Net Weight Net Weight No ofOrig. Organism (mg) 

Treatment Rep Units: Weight (g) Weight (g) (g) (g) Organisms (mg) (Original) 

A 0.00000 0.00000 8 0.000 0.0000 

B 0.00000 0.00000 8 0.000 

32% c 0.00000 0.00000 8 0.000 

D 0 0.00000 0.00000 8 0.000 

E 0.00000 0.00000 8 0.000 

A 0.00000 0.00000 #DIV/0! #DIV/0! 

B 0.00000 0.00000 #DIV/0! 

c 0.00000 0.00000 #DIV/0! 

D 0.00000 0.00000 #DIV/01 

E 0.00000 0.00000 #DIV/0! 

Blank 0.93373 0.93374 0.00001 

Number of 

Surv. 

Organisms 

0 0 0 

0 

0 
TEST ORGANISM LENGTHS, WEIGHTS, AND LOADING 

Project Number. 8503-132-265-006 Species: Fathead minnow 

MeanW.J MeanW.J 

Adjusted Original Treatment 

Length Tare Gross Net Weight Net Weight No ofOrig. Organism (mg) 

Treatment Rep Units: Weight(g) Weight (g) {g) {g) Organisms (mg) (Original) 

Summary Statistics for Survival Data 

Treatment N Min Max Mean SD c.v. 

MH 4 1.0 1.0 1.0000 0.0000 0.000% 

0.5% 5 0.9 1.0 0.9714 0.0639 6.577% 

1% 5 0.9 1.0 0.9500 0.0685 7.207% 

{Z 5% 5 0.9 1.0 0.9714 0.0639 6.577% 

5 1.0 1.0 1.0000 0.0000 0.000% 

32% 5 0.0 0.0 0.0000 0.0000 #DIV/0! 

Summary Statistics for Growth Data (dry wt per original) 

Treatment N Min Max Mean SD c.v. 

MH 4 0.543 0.634 0.5862 0.0394 6.727% 

0.5% 5 0.501 0.658 0.5715 0.0665 11.640% 

1% 5 0.420 0.609 0.5013 0.0783 15.620% 

5% 5 0.410 0.594 0.5038 0.0817 16.210% ;_.so,{, CNT 5 0.499 0.596 0.5460 0.0367 6.713% 

32% 5 0.000 0.000 0.0000 0.0000 #DIV/0! 

- - - - -

Summary Statistics for Growth Data {dry wt per surviving organism} 

Treatment N Min Max Mean so C.V. 

MH 4 0.543 0.634 0.5862 0.0394 6.727% 

0.5% 5 0.521 0.658 0.5883 0.0538 9.144% 

s1 1% 5 0.480 0.609 0.5255 0.0507 9.657% 5% 5 0.410 0.594 0.5198 0.0840 16.163% 

5 0.499 0.596 0.5460 0.0367 6.713% 

32% 0 0.000 0.000 #DIV/0! #DIV/0! #DIV/0! 

1/1-p tj-Cf 

Number of 

Surv. 

Organisms 
Page 000095 of 117 SAN 

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

Page l!/. of/<{ 

. 'f--":?JJ. g T;/ctl_~ 

CIPr~· ;Je"'i/1;7/:if:. 

File: 265006po.dat Transform: NO TRANSFORMATION 

Summary Statistics on Data TABLE 1 of 2 

GRP IDENTIFICATION N MIN MAX MEAN 

1 0 4 0.5430 0.6340 0.5865 

2 0.5 5 0.5010 0.6580 0.5714 

3 l 5 0.4200 0.6090 0.5014 

4 S't, 5 5 o.4loo o.594o o.5o38 

5 ~% CNT 5 0.4990 0.5960 0.5460 

------------------~--------------------------------------------------------

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

File: 265006po.dat Transform: NO TRANSFORMATION 

Summary Statistics on Data TABLE 2 of 2 

GRP IDENTIFICATION VARIANCE SD SEM c.v. % 

1 0 0.0015 0.0393 0.0197 6.7033 

2 0.5 0.0045 0.0668 0.0299 11.6828 

3 1 0.0061 0.0783 0.0350 15.6257 

4 ')~ 5 0.0067 0.0818 0.0366 16.2414 

5 _0% CNT 0. 0013 0. 0365 0. 0163 6. 685.6 

--------------QD------------------------------------------------------------

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

File: 265006po.dat Transform: 

D = 0.0791 

w = 0.9604 

Critical w 

w 

Shapiro - Wilk•s Test for Normality 

0.8840 (alpha 

0.9160 (alpha 

0.01 

0.05 

N = 24) 

N 24) 

NO TRANSFORMATION 
Page 000096 of 117 SAN 

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

Page!{of /~ 

f-~tJ Y/~/d( <l W; lt'UK/'2i7 /cf 

File: 265006po.dat Transform: NO TRANSFORMATION 

Bartlett's Test for Homogeneity of variance 

Calculated B1 statistic = 3.4425 (p-value = 0.4867) 

Data PASS B1 homogeneity test at 0.01 level. Continue analysis. 

Critical B = 13.2767 

9.4877 

(alpha 

(alpha = 

0.011 df = 

0.05, df 

4) 

4) 

Using Average Degrees of Freedom 

(Based on average replicate size of 4.80) 

Calculated B2 statistic = 

Data 8B2 homogeneity 

2.7543 (p-value = 0.5997) 

test at 0.01 level. Continue analysis. 

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

File: 265006po.dat Transform: NO TRANSFORMATION 

SOURCE DF 

Between 4 

Within (Error) 19 

Total 

Critical F 

23 

= 4.5003 

2.8951 

ANOVA Table 

(alpha = 

(alpha 

ss 

0.0278 

0.0791 

0.1069 

0.01, df 

0.05, df 

4,19) 

4,19) 

MS F 

0.0069 1.6665 

0.0042 

(p-value = 0.1992) 

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha= 0.05) 

-------------------------------------------------------------------------------------··---
Page 000097 of 117 SAN 

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

File: 265006po.dat Transform: 

Bonferroni t-Test TABLE 1 OF 2 

Page~of/f 

~;;.rJ i/~:tlo~ {J.fr·. fr{U)gjziJt( 

NO TRANSFORMATION 

Ho: Control<Treatment 

TRANSFORMED 

MEAN 

MEAN CALCULATED IN SIG 

GROUP IDENTIFICATION ORIGINAL UNITS t STAT 0.05 

1 0 0. 5865 0. 5865 

2 o.5 0.5714 0.5714 0.3488 I 

4

3 ~"'' 51 0. 5014 0. 5014 l. 9658 J-)1(') ( 

J ~ 0.5038 0.5038 1.9104 

Bonferroni --~------------~~~-=~=------~~=::~-------------~~=::~--------~~===~----- t critical value = 2.4334 (1 Tailed, alpha= o.os, df = 4,19) 

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

File: 

GROUP 

265006po.dat Transform: NO TRANSFORMATION 

Bonferroni t-Test 

IDENTIFICATION 

TABLE 2 OF 2 Ho: Control<Treatment 

NUM OF 

REPS 

MIN SIG DIFF % OF DIFFERENCE 

(IN ORIG. UNITS) CONTROL FROM CONTROL 

l 0 4 

2 0. 5 5 0 .1053 18. 0 0. 0151 

3 .,., 1 5 0.1053 18.0 0.0851 

4 ..., ,.., 5 5 0.1053 18. 0 0. 0827 

5 . _>v.(% CNT 5 0. 1053 18. 0 0. 0405 

---------------~-----------------------------------------------------------
Cone. ID 1 

Page 000098 of 117 SAN 

r?;o :?-t ?"'2---~5-o oy, 

2 

Page 11 of.{( 

/1-6f-.J rg / ~)o·rt a rr; fr{tfJ g{ ')17 tor 3 4 

Cone. Tested 0 0.5 1 5 

Response 1 .569 .622 .42 .45 

Response 2 .634 .658 .609 .481 

Response 3 • 6 .521 .52 .594 

Response 4 .543 .501 .429 .584 

Response 5 .555 .529 .41 

*** Inhibition Concentration Percentage Estimate *** 

Toxicant/Effluent: effluent 

Test Start Date: 8/15/08 Test Ending Date: 8/22/08 

Test Species: P. promelas 

Test Duration: 7 days 

DATA FILE: 265006go.icp 

OUTPUT FILE: 265006go.i25 

Cone. 

ID 

1 

2 

3 

4 

5 

Number· 

Replicates 

4 

5 

5 

5 

5 

Concentration 

% 

0.000 

0.500 

1. 000 

5.000 

32.000 

Response 

Means 

0.587 

0.571 

0.501 

0.504 

0.000 

Std. 

Dev, 

0.039 

0.067 

0.078 

0.082 

o.ooo 

5 

32 

0 

0 

0 

0 

0 

Pooled 

Response Means 

0.587 

0.571 

0.503 

0.503 

0.000 

The Linear Interpolation Estimate: 8.3696 Entered P Value: 25 

Number of Resamplings: 80 

The Bootstrap Estimates Mean: 

Original Confidence Limits: 

Expanded confidence Limits: 

Resampling time in Seconds: 

7.3416 Standard Deviation: 1.3265 

Lower: 4.4158 Upper: 10.0959 

Lower: 2.4389 Upper: 10.9590 

0.00 Random_seed: -334101559 
Page 000099 of 117 SAN 

Page: I g If 1i 

FCETL QA Form No. 15- ~ 

Effective: 5/90 

~ltr/o8 SUBJECT: DAILY LOG 

ALL ENTRIES MUST BE INITIALLED WITH OATE ANO TIME: 

"' .(i) 

PteP 1'2\- Dt l),~ lo £ ~('a"" Uo~ t 

b 

~~- u.)ll{ ~ ~~\,\ ..Por lt' d tr!) ~ .~~I ~ ~Q-i,J~ ..., Jq!,~) 

b 00 """ ~. iJ>P(o~ ·l.J~f';· {l~ -f., T7--' ll)$ t.J. 

M.-6..L tlw-J ( {Lt.;) # 5: 4--l. i' l 

-', . • a.Ll. D 0"2..."3 loa $'W G!SI '"'-~ f,;. ~t; -f_.r-f 

(~ 2:za \It/ ' 

../ 

Z>. 0 2. 5 ?;; 1-t (), O'Z5q r ... i·f D."D~J.~ x:..., 

IDS)- I 100 Mj -. 

J 7.-Cww'l 

JIG = "2. .% 4 

\../ 

4 .. ..tr..d ... Al • ,"'Sl ;::. "[ v~ f ~ ~ 1 J 

I I . l 

m tv.~ .1 ul.e., ... {d'_~,.J A ..\ P( D ~tert""f Sioll'l .s{A:JII!.f~ I /"'a.V~ ~ /:.lJ_J_,.J 60 tV\-t f.o~i'tY·trP.A ro ~z..L .JV~.DJ lut-1>} 

· I 11.5}~ If /..('. <. /)Je~p~. 

/ .. /'~ .uxfu~ (JJAJ S L';) Sv[l\ 

8 J.4.) 1ft~ /1J'i! Vf 

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Page 000100 of 117 SAN 

10e Structure of Carbon Nanotubes 

Single wall carbon nanotubes (SWNT) are an allotrope of sp2 hybridized carbon similar to fullerenes. The 

structure can be considered as a cylindrical tube comprised of 6-membered carbon ring as in graphite. 

The cylindrical nanotubes usually have at least one end capped with a hemisphere of the buckyball or 

fullerene structure. Typical structures are shown below: 

m.m.•· f~Hl1 
Page 000101 of 117 SAN 

The detailed structure of the nanotubes varies with diameter and is defined by the chiral angle as shown 

in the diagram below. The distance from the origin defines the diameter of the tube. 

Carbon nanotubes produced by SouthWest Nanotechnologies have the following typical properties: 

Parameter CG 100 SG 65 SG76 

Tube Diameter (nm) from OA and 1.0 ± 0.3 0.8 ± 0.1 0.9 ± 0.2 

AFM 

Tube Length (nm) from AFM 

Mode 800 900 Not Measured 

Range 400- 2,300 400- 2,500 

Carbon Content{%) >90 >90 >90 

SWNT Content {% carbon as 75 80 80 

SWNT) 

Dominant Chiralities {6,5), (7,5), (6,5) (7,6) 

(7,6) 

Chirality and Tube Diameter 

The chirality of the carbon nanotube dictates its diameter. In the manufacturing of carbon nanotubes 

many different chiralities of carbon nanotubes are formed. With the CoMoCat® process it is possible to 

vary the reactor conditions to manufacture products with dominant chiralities. Various techniques are 
Page 000102 of 117 SAN 

available to determine the chirality of the tubes present. The dominant chiralities for each grade are 

shown in the table above. The most common techniques are: 

a) Optical Absorption measurements in the UV-visible-Near IR region of the spectrum 

b) Raman Spectroscopy in the region ofthe radial breathing mode of the spectrum 

c) Fluorescence spectroscopy. 

Optical absorption measurements are used routinely in the manufacturing operation to monitor the 

chirality of the product produced. Typical Spectra for the three grades of material currently produced 

are shown below. The main chiralities can be determined from the peaks caused by the Van Hove 

transitions in the Density of States and are marked on the Spectra. 

Typical Optical Absorbance Spectrum fot SWeN~ SG 65 

2.5 

E' 

c 

0 

~ 2.0 

2l 

c 

-e "' 

0 1.5 rJ) 

.0 

<t: 

"0 

"iii -~ 1.0 

E 

0 

z 

0.5 

400 

(6,5) 

600 

(6,5) 

(7,6) 

(7,6) 

800 1000 1200 1400 

Wavelength (nm) 

The Optical absorbance spectrum for SG65 is dominated by the large peak associated with (6,5) chirality 

tubes and a smaller peak for the (7,6) tubes. As can be seen from the spectrum the peaks show in two 

distinct regions of the spectrum. These two regions correspond to two different electronic transitions 

known as 511, (850- 1400 nm) and 522, (550- 750 nm). Tubes are identified by their transitions in 

both regions of the spectrum from published tables, (see attachment.) Once the chiralities have been 

established the diameter can also be determined from published tables: The nominal diameter of the 

(6,5) tube is 0.857 nm and that of the (7,6) tube 0.895 nm thereby giving an average diameter of 0.8±0.1 

nm. 
2.0 

'E 

c 1.8 

0 

00 

!::::-

Ql 1.6 (.) 

c 

rn 

-e 

0 1.4 Ill 

.c 

<( 

"0 

Ql 1.2 

.!::! 

a; 

E 1.0 0 

z 

0.8 

Page 000103 of 117 SAN 

Typical Optical Absorbance Spectrum fot SWeN~ SG 76 

400 600 

(8,7) 

[1,6) (8,6) 

800 1000 

Wavelength (nm) 

[1,6) 

1200 1400 

Similarly for the SG76 material the dominant chiralities are for (7,6), (8,6) and (8,7) tubes. From tables 

the diameters of these tubes are 0.895, 0.966 and 1.032 nm respectively. 

Typical Optical Absorbance Spectrum fot SWeN~ CG-100 

2.5 

'E 

c (6,5) 0 

00 2.0 !::::-

Ql 

(.) [1,5) c 

rn [1,6) -e 

0 1.5 Ill 

.c 

<( 

"0 

Ql 

.!::! 1.0 a; (8,7) 

E 

0 

z 

0.5 

400 600 800 1000 1200 1400 

Wavelength (nm) 

CG 100 differs from the other materials in that it has a wider range of chiralities and hence diameters. 

Based on the OA spectrum the diameter of the tubes has been determined to be in the range of 1.0±0.3 

nm. 
Page 000104 of 117 SAN 

Tube Length 

Tube length has been measured directly using an Atomic Force Microscope. For SG 65 material the 

mode (most common frequency) of the distribution is approximately 900 nm with a range from 

approximately 400 10 2500 nm, with >95% ofthe tubes falling within the range Of 500 to 2,000 nm. 

Length Distribution SG 65 

3 a 

! 

0 B :!a 

5 

z 

1 a 

a 
Page 000105 of 117 SAN 

The length of CG 100 tubes was measured by the 'same method. 

CG Length distribution 

IIICG 191 

Total Tubes > 250 

20" 

II. ... 

##~#~~,~~'''~'''' LerQ:h(nm) 

The most common length frequency was measured as 800nm with a typical range from 400 to 2,300 

nm. Two outliers were observed at approximately 3 and 3.5 !J.m. 

The length of SG76 tubes has not been determined, but is expected to be similar to SG 65 and CG 100. 
Page 000106 of 117 SAN 

Carbon and SWNT Content 

Purity of the material is determined by Thermogravimetric Analysis. In this process the material is 

heated in a furnace at a rate of 5 C degrees/minute while the sample weight is continuously monitored. 

An example TGA is shown below. The green curve represents the loss in weight of the sample as it is 

heated while the blue curve is the derivative of the weight loss. 

Sample: SG6S.000..0010 Run Date: 2()08..06.14 09:17 

100-~----------------------------------------------------------r 1.2 . ··~···- ---..... 149.83'C' 

98.46% 

T1SS2.46 

Resldufil messa4.829 

1.0 

6 

" 

-

~ 

60-i l: 

.21 

~ 

.~ .... 

0.4 (3 

40 

(I 

:w j \ " ____ __.~ ~----~---

Catalyst oxides 

and carbides 

;/ 

340 540 

Temperature ("C) 

-~"""""~----· 0.0 

748.02'C 

3.913% 

~-----------··+-··'- ---.---·---····---·-·-+ 

640 740 

From the TGA it is possible to estimate the proportion of single wall nanotubes, other forms of 

graphitic carbon and residual catalyst 

• The first derivative peak is a result of the oxidation of single wall carbon 

nanotubes. Tl% is representative of the proportion of single wall nanotubes in 

the material 

• The second derivative peak above T1 represents the oxidation of other forms of 

graphitic carbon formed as a by-product in the manufacturing process. 

• The residual mass at 62s•c is representative of the non carbonaceous material, 

primarily metal oxides and carbides from the catalyst. 
Page 000107 of 117 SAN 

Thus from the TGA we can determine the total carbon content, the percentage of carbon which is 

comprised of single wall carbon nanotubes and the residual mass. 

Additional Structural Data 

As mentioned above, Raman Spectroscopy can also be used to determine the distribution of chiralities in 

the material. This is a very complicated procedure and to date has only been conducted on early SG 65 

type material. This work was conducted independently at Universidade Federal de Minas Gerais, Belo 

Horizonte, Brazil by Jorio, A et. a/ published in Physical Review B 72, 075207 {2005.} The Raman study 

confirmed the dominant peaks seen in the OA spectrum for SG 65 plus a high proportion of (7,5) tubes. 

The (7,5) tube would be seen as a shoulder on the high wavelength side of the (6,5) peak. The (6,5) 

Optical Absorbance Spectrum shown earlier does not show this peak. This is due to tighter control of 

manufacturing conditions for this material, which has significantly reduced the presence of this 

particular tube. 

A total of 35 different chiralities were identified. 

The three grades of material have also been characterized by Transmission Electron Microscopy, (TEM.) 

Typical TEMs for the three grade SG 65, SG 76 and CG 199 are shown below: 

Typical TEM Image for SWeNT® SG 65 
Page 000108 of 117 SAN 

Typical TEM Image for SWeNT® SG 76 

Typical TEM Image for SWeNT® CG-100 
Page 0001 09 of 117 SAN 

These TEM images show the strong tendency for nanotubes to form into bundles (or ropes.) The degree 

of roping is highly dependant on the conditions. For example some surfactants and polymers such as 

Sodium cholate or sodium dodecyl sulphate tend to break up bundles, whilst other surfactants, such as 

Triton X-100 tend to preserve the rope structure. Ropes may vary in diameter from 2 to 3 nm upto 20 

to 30 nm. 
Page 000110 of 11z7 SAN 

SECTION 1 PRODUCT /DENT/FICA TION 

Material Safety Data Sheet 

SWeNT® Freeze*Dried Powder 

Single-Wall €arbon Nanotubes 

PRODUCT NAME: Carbon Nanotube Freeze-Dried Powder - SG65, SG7 6, CG 1 00, CG200 

OTHJ:R/GENERIC NAMES: SWNT; SWCNT, Single-Wall Carbon Nanotubes 

MANUFACTURER: SouthWest NanoTechnologies, Inc. 

2501 Technology Place 

Norman, OK 73071 -11 02 

+1 405.217.8388- Tel 

+1 405.217.8389- Fax 

http://swentngno.com 

safety@swentnano.com 

SECTION 2 COMPOSITION AND INFORMATION ON INGREDIENTS 

Ingredient Name 

Single-Wall Carbon Nanotubes 

Metallic impurities (including Molybdenum and Cobalt) 

CAS Number 

None 

Various 

Weight o/o 

85-99 

1-15 

Trace impurities and additional material names not listed above may also appear in Section 15 towards the end of 

the MSDS. These materials may be listed for local "Right-To-Know" compliance and for other reasons. 

This material is considered as hazardous under OSHA regulations. 

SECTION 3 HAZARDS IDENTIFICATION 

EMERGENCY OVERVIEW: Product is a freeze-dried po'wder. May cause eye, skin and respiratory tract 

irritation. The complete physical and toxicological properties of this material have not been fully evaluated. 

Potential Health Hazards 

SKIN: This material may cause skin irritation. Use proper precautions. 

EYES: This material may cause eye irritation. Use proper precautions. 

INHALATION: This material may cause irritation to the mucous membranes and upper respiratory tract. The product 

presents an increased inhalation hazard because of the small particle size. Use proper precautions. 

INGESTION: Not a probable route of exposure. This material may be harmful if swallowed. 

DELAYED EFFECTS: None known. 

Ingredients found on one of the OSHA designated carcinogen lists are listed below. 

INGREDIENT NAME 

Cobalt Compounds 

NTP STATUS IARC STATUS 

2B 

OSHA LIST 
Page 000111 of 117 SAN 

SECTION 4 FIRST AID MEASURES 

SKIN: Wash with soap and water. Get medical attention if irritation develops or persists. 

EYES: Flush eyes with plenty of water. Get medical attention if irritation develops or persists. 

INHALATION: Remove to fresh air. If not breathing, give artificial respiration. Get medical attention if irritation develops 

or persists. 

INGESTION: If person is conscious, rinse mouth with water. Do not induce vomiting unless directed to do so by a physician. 

ADVICE TO PHYSICIAN: No specific advice, treat symptomatically. 

SECTION 5 FIRE FIGHTING MEASURES 

FLASH POINT: Not determined. 

FLASH POINT METHOD: Not applicable. 

AUTOIGNITION TEMPERATURE: Not determined. 

UPPER FLAME LIMIT (volume o/o in air): Not applicable. 

LOWER FLAME LIMIT (volume % in air): Not applicable. 

FLAME PROPAGATION RATE (solids): Not determined. 

OSHA FLAMMABILITY CLASS: Not determined. 

DECOMPOSITION PRODUCTS.: Carbon Monoxide, Carbon Dioxide. 

UNUSUAL FIRE AND EXPLOSION HAZARDS: 

Airborne dust from the dried dispersion in an enclosed space and in the presence of an ignition source may 

constitute an explosion hazard. 

SPECIAL FIRE FIGHTING PRECAUTIONS/INSTRUCTIONS: 

As in any fire, wear NIOSH/MSHA approved positive pressure self-contained breathing apparatus and full 

protective clothing. 

SECTION 6 ACCIDENTAL RELEASE MEASURES 

IN CASE OF SPILL OR OTHER RELEASE: 

Use appropriate personal protection during clean up. Remove mechanically by a method that minimizes the generation 

of airbourne dust (HEPA equipped vacuum, wet mopping etc.) Abosrb material and place in appropriate containers 

for disposal. Do not allow spilled material or wash water to enter sewers, surface water, or ground water. 

SpiUs and releases may have to be reported to Federal and/or local authorities. See Section 15 regarding 

reporting requirements. 

SECTION 7 HANDLING AND STORAGE 

NORMAL HANDLING: (Always wear recommended personal protective equipment.) 

Use local exhaust or general room/dilution ventilation sufficient to maintain exposure below 

permissible exposure limits (29 CFR 191 0.1 001 for asbestos). If possible, use in a closed well 

ventilated area (e.g. fume hood). 

STORAGE RECOMMENDATIONS: 

To maintain product quality, store between 40 and 100 °F. 
Page 000112 of 117 SAN 

SECTION 8 EXPOSURE CONTROLS AND PERSONAL PROTECTION 

ENGINEERING CONTROLS: 

General- room ventilation is adequate for storage and ordinary handling. Use local exhaust at points of use to 

maintain exposure below the PEL/TL V exposure limits. 

PERSONAL PROTECTIVE EQUIPMENT 

SKIN PROTECTION: 

Impervious garments and gloves. 

EYE PROTECTION: 

Wear chemical goggles that conform to ANSI Z87.1 under normal conditions. 

RESPIRATORY PROTECTION: 

If there is potential for inhalation of dust, vapors, or aerosols wear a full-face NIOSH approved respirator with N 1 00 

cartridges or better. 

The respirator must be selected based on contamination levels and use conditions found in the workplace. 

Use conditions must not exceed the working limits of the respirator. The respirator must be used in 

accordance with the OSHA respiratory protection standard (29 CFR 1 91 0.1 34). 

ADDITIONAL RECOMMENDATIONS: 

Provide safety showers and eyewash stations in close proximity to the work area. 

EXPOSURE GUIDELINES 

INGREDIENT NAME ACGIH TLV 

Single-Wall Carbon Nanotubes Not available. 

OSHA PEL 

Not available. 

OTHER LIMIT 

None. 

Insoluble Molybdenum Compounds, as Mo TWA= 10 mg/m3 (8-

hr day, inhalable 

fraction), 

TWA= 3 mg/m3 (8-hr 

day, respirable 

fraction) 

Cobalt Compounds, as Co TWA= 0.02 mg/m3 

* 

** 

*** 

= Limit established by SWeNT for internal use. 

= Workplace Environmental Exposure Level (AIHA). 

= Biological Exposure Index (ACGIH). 

(8-hr day) 

TWA= 0.05 mg/m3 

(8-hr day) 

None. 

15 ug/L urine *** 

1 ug/L blood *** 

PEL values represent limits established by the 1989 Air Contaminants Rule (29 CFR 1910.1000, Subpart Z, Table 

Z-1-A) which was subsequently revoked on June 30, 1993. Several states continue to enforce Table Z-1-A limits. 

OTHER EXPOSURE LIMITS FOR POTENTIAL DECOMPOSITION PRODUCTS: None. 

SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES 

APPEARANCE: Black powder. 

PHYSICAL STATE: Solid. 

ODOR: None. 

SPECIFIC GRAVITY (water = 1.0): 

SOLUBILITY IN WATER (weight%): 

pH: 4-10 

BOILING POINT: Not determined. 

MELTING POINT: Not applicable. 

VAPOR PRESSURE: Not applicable. 

Not determined. 

Insoluble. 

VAPOR DENSITY (air= 1.0): Not determined. 

EVAPORATION RATE: Not determined. 

% VOLATILES: Not determined. 

FLASH POINT: Not determined. 

COMPARED TO: 

(Flash point method and additional flammability data are found in Section 5.) 

Not applicable. 
SECTION 1 0 STABILITY AND REACTIVITY 

STABILITY {CONDITIONS TO AVOID) 

Normally stable. 

INCOMPATIBILITIES: 

None known. 

HAZARDOUS DECOMPOSITION PRODUCTS: 

Page 000113 of 117 SAN 

Thermal decomposition products may include carbon monoxide, carbon dioxide and oxides of metallic impurities 

(including molybdenum and cobalt). 

HAZARDOUS POLYMERIZATION: 

Will not occur. 

SECTION 11 TOXICOLOGICAL INFORMATION 

IMMEDIATE {ACUTE) EFFECTS: 

No data available. 

DELA YEO {SUBCHRONIC AND CHRONIC) EFFECTS: 

No data available. 

OTHER DATA: 

None. 

SECTION 12 ENVIRONMENTAL INFORMATION 

No data available. 

SECTION 13 DISPOSAL CONSIDERATIONS 

Is the unused product a RCRA hazardous waste if discarded? No. 

If yes, the RCRA ID number is: Not applicable. 

OTHER DISPOSAL CONSIDERATIONS: 

Disposal of this product is not allowed by federal, state and local government regulations. It must be destroyed by a suitable method 

including, but not limited to, incineration. 

The information offered here is for the product as shipped. Use and/or alterations to the product, such as mixing with 

other materials, may significantly change the characteristics of the material and alter the RCRA classification and the 

proper disposal method. 

SECTION 14 TRANSPORTATION INFORMATION 

US DOT HAZARD CLASS: Not regulated. 

US DOT ID NUMBER: Not applicable. 

For additional information on shipping regulations affecting this material, contact the information number found on 

Section 1. 

SECTION 15 REGULATORY INFORMATION 

TOXIC SUBSTANCES CONTROL ACT {TSCA) 

TSCA INVENTORY STATUS: This material is manufactured under the terms of the TSCA LOREX exemption at 40 CFR 

723.50(c)(2), and under the requirements of 40 CFR723.50U). Further, according to 723.50(k)(2) this material cannot be 

further distributed until it has been reacted, incorporated into an article, or otherwise rendered into a physical form or state. 

OTHER TSCA ISSUES: None. 

SARA TITLE 111/CERCLA 

"Reportable Quantities" (RQs) and/or "Threshold Planning Quantities" (TPQs) exist for the following ingredients. 

INGREDIENT NAME SARA/CERCLA RQ (lb) SARA EHS TPQ (lb) 

No ingredients listed in this section. 

Spills resulting in the loss of any ingredient at or above its RQ requires immediate notification to the 

National Response Center (1-800-424-8802) and to your Local Emergency Planning Committee. 

------------------------------------------------------------------------------------------------------
SECTION 311 HAZARD CLASS: 

Page 000114 of 117 SAN 

Immediate (Acute). 

The following. ingredients are SARA 313 "Toxic Chemicals". CAS numbers and weight percents are found in 

Section 2. 

INGREDIENT NAME 

Cobalt Compounds 

STATE RIGHT-TO-KNOW 

COMMENT 

Elemental metal content< 0.1 0%. 

In addition to the ingredients found in Section 2, the following are listed for state right-to-know purposes. 

INGREDIENT NAME WEIGHT % COMMENT 

No ingredients listed in this section. 

ADDITIONAL REGULATORY INFORMATION 

WHMIS CLASSIFICATION (CANADA): 

Not determined. 

FOREIGN INVENTORY STATUS: 

All components of this product are listed on the following inventories: 

Australian (AICS) 

Canadian (DSL) 

Chinese (IECSC) 

European (EINECS) 

Japanese (ENCS) 

Korean (KECI) 

SECTION 16 OTHER INFORMATION 

CURRENT ISSUE DATE: 

PREVIOUS ISSUE DATE: 

August 1 0, 2009 

April 22, 2009 

CHANGES TO MSDS FROM PREVIOUS ISSUE DATE ARE DUE TO THE FOLLOWING: 

Additional product names were added. 

OTHER INFORMATION: 

None. 

DISCLAIMER: THE INFORMATION HEREIN IS PROVIDED IN GOOD FAITH, AND IS BELIEVED TO BE ACCURATE. 

NO WARRANTY, EXPRESSED OR IMPLIED, IS MADE REGARDING THE ACCURACY OR COMPLETENESS 

OF THIS INFORMATION. FURTHERMORE, SWENT MAKES NO REPRESENTATION OR WARRANTY THAT THE SAMPLE 

IS FIT FOR ANY PARTICULAR PURPOSE AND SWENT EXPRESSLY DISCLAIMS ANY WARRANTY OF 

MERCHANTABILITY. THIS DOCUMENT IS INTENDED ONLY AS A PRECAUTIONARY GUIDE FOR THE APPROPRIATE 

HANDLING OF THE MATERIAL BY A PROPERLY TRAINED PERSON USING THIS PRODUCT, WHO MUST EXERCISE 

INDEPENDENT JUDGMENT IN DETERMINING HANDLING OF THIS PRODUCT AND THE APPROPRIATENESS OF THIS 

PRODUCT FOR ANY PURPOSE. SWENT SHALL NOT BE LIABLE FOR CONSEQUENTIAL DAMAGES OR FOR DAMAGE 

TO PERSONS OR PROPERTY RESULTING FROM ITS USE. NOTHING HEREIN SHALL BE CONSTRUED AS A 

RECOMMENDATION FOR USE IN VIOLATION OF ANY PATENT. SWENT® IS A TRADEMARK OF SOUTHWEST 

NANOTECHNOLOGIES, INC. CAUTION: POTENTIAL HAZARDS OF THIS PRODUCT ARE UNKNOWN. 

MANUFACTURED UNDER U.S. PATENT NOS. 6,333,016, 6,413,487, 6,955,800 AS WELL AS OTHER PENDING 

PATENT APPLICATIONS IN THE U.S. AND AROUND THE WORLD. 
Page 

Si-ngle-Wall Carb,on Nanot

Manufactured under U.S. Patent Nos. 

f) and 695580 _cts we II 

as other pendlngpatent applications In 

the U.S. anda(ouhdthe world. 

CAUTION: 

Potential hazards'ofthis product are: Unknown. 

This product can only be_ used under TSCA R&D conditl ons. 

Compliance Withtheenclosed MSD,S is advised. 
Page 000116 of 117 SAN 

State of Ohio 

~ Environmental Protection Agency 

STREET ADDRESS: 

Lazarus Government Center 

122 S. Front Street 

Columbus, Ohio 43215 

March 23, 2005 

CERTIFIED MAIL 

Mr. Fred Sigg 

Von Roll America, Inc.;· 

1250 Saint George Street 

East LiverpooJ,. Qhio 43920 ... 

Dear Mr. Sigg: 

TELE: (614) 644-3020 FAX: (614) 644-3184 

· Yiww.epa.stata.oh.us 

MAILING ADDRESS: 

·P.O. Box 1049 

Columbus, OH 43216-1049 

Re: Ohio Hazardous Waste Permit Renewal 

vo·n Roll America, Inc. 

U.S. EPA 10 No.: OHD 980 613 541 

Ohio 10 No.: 02-15-0589 

Here is the renewed Ohio Hazardous Waste Facility Installation and Operation Permit 

(Permit) for Von Roll America, ·Inc. I have also enclosed a copy of the responsiveness 

summary Ohio EPA prepared in response to written comments the Agency received 

concerning the Part 8 permit application. The Permit is effective today, March 23, 2005. 

The date-stamped, page-numbered copy of the Part B permit application is also 

enclosed. 

Please remember that according to Rule 37 45-50-36 of the Ohio Administrative Code 

your annual hazardous waste permit fee of ·.j , ·::' :· will be due on March 23,, 2006. 

Ohio EPA will try to notify you before this fee is due, but it is your responsibility to make 

.... sure it gets paid on time. 

As a party to this permit proceeding, you may appeal this Permit to the Environmental 

Review Appeals Commission (ERAC) no later than 30 days after the public notice (See 

Ohio Revised Code§ 3745.04). You may file your appeal with ERAC at the following 

address: Environmental Review Appeals Commission, 309 South Fourth Street, Room 

222, Columbus, Ohio 43215. 

If you file an appeal, you must put itin writing. Your appeal must explain why you are 

appealing the action and the grounds you are using for your appeal. You must send a 

copy of the appeal to the director of the Ohio Environmental Protection. Agency no later 

than three (3) days after you file it with ERAC. 

@ Print~donRecycledPaper 

Bob Taft, Governor 

Bruce Johnson, Lieutenant Governor 

Joseph P. Konceiik, Director 

Ohio EPA Is an Equal Opportunity Employer 
Mr. Fred Sigg 

1 Von Roll America, Inc. 

March 23, 2005 

Page Two 

Page 000117 of 117 SAN 

If you have any questions concerning compliance, do not hesitate to call Patricia Natali 

of Ohio EPA's Northeast District Office at (330) 385-8447. 

Sincerely, 

~ Pamela 

~ S. Allen, Manager 

Regulatory and Information Services 

Division of Hazardous Waste Management 

Attachments 

cc: Edwin Lim, Mgr., ERJ}.S, DHWM 

Jeremy Carroll/John Nyers, ERAS, DHWM 

Harriet Croke, US EPA, Region V 

Frank Popotnik/Patricia Natali, DHWM, NEDO 

Mike Settles, Public Interest Center, Ohio EPA 

file 

g:\users\lterry\VonRcii03'05FinaiRenewalcoverLtr.wpd 

.\