Attachment FEDSPICRRenewal(EPAICR#2249.03;OMB#2070‐0176)Assay 2009 Assay Cost ($) (a-1) Adjusted Assay Cost ($) (a-2) Paperwork Burden Cost ($) (b) Management Burden Cost ($) (c) Technical Burden Cost ($) (d) Clerical Burden Cost ($) (e) Management Burden (Hours) (f) Technical Burden (Hours) (g) Clerical Burden (Hours) (h) Total Burden (Hours) (i)Amphibian Metamorphosis (Frog) 82,065.50 87,780.79 30,723.28 6,144.66 19,970.13 4,608.49 54.62 359.89 138.19552.70Androgen Receptor Binding (RatProstrate)23,814.00 25,472.48 8,915.37 1,783.07 5,794.99 1,337.31 15.85 104.43 40.10160.38Aromatase (Human Recombinant) 29,800.00 31,875.36 11,156.38 2,231.28 7,251.64 1,673.46 19.84 130.68 50.18200.70Estrogen Receptor Binding (Rat Uterine) 23,329.00 24,953.70 8,733.80 1,746.76 5,676.97 1,310.07 15.53 102.31 39.28157.12Estrogen Receptor TranscriptionalActivation (Human Cell Line)23,867.00 25,529.17 8,935.21 1,787.04 5,807.89 1,340.28 15.89 104.67 40.19160.74Fish Short-term Reproduction 111,230.50 118,976.93 41,641.93 8,328.39 27,067.25 6,246.29 74.04 487.79 187.30749.12Hershberger (Rat) 45,583.00 48,757.54 17,065.14 3,413.03 11,092.34 2,559.77 30.34 199.90 76.75306.99Female Pubertal (Rat) 96,967.00 103,720.07 36,302.02 7,260.40 23,596.32 5,445.30 64.54 425.24 163.28653.06Male Pubertal (Rat) 100,350.00 107,338.67 37,568.53 7,513.71 24,419.55 5,635.28 66.79 440.07 168.97675.84Steroidogenesis (Human Cell Line) 17,450.00 18,665.27 6,532.84 1,306.57 4,246.35 979.93 11.61 76.52 29.38117.52Uterotrophic (Rat) 41,242.00 44,114.22 15,439.98 3,088.00 10,035.99 2,316.00 27.45 180.86 69.45277.76Total Burden and Costs: 595,698.00$ 637,184.20$ 223,014.47$ 44,602.89$ 144,959.41$ 33,452.17$ 396.51 2,612.35 1,003.06 4,011.92(g) Based on total estimated technical costs (j) divided by the loaded labor rate for technical staff ($55.49).(h) Based on total estimated clerical costs (j) divided by the loaded labor rate for clerical staff ($33.35).(i) Total Burden Hours is used in Table 6 of this ICR for estimating the burden & costs related to data generation paperwork activities.http://www.bls.gov/data/inflation_calculator.htm.(b) Based on 35% of the total estimated test costs.(c) Calculated as 20% of (b), which is 35% of the total estimated test costs.(d) Calculated as 65% of (b), which is 35% of the total estimated test costs.(e) Calculated as 15% of (b), which is 35% of the total estimated test costs.(f) Based on total estimated managerial costs (j) divided by the loaded labor rate for managers ($112.49).Calculations for Paperwork Burden and Costs for Data Generation ActivitiesEstimated Burden(a-1) Estimated costs for these assays are from Table 8 in the EPA report entitled: "Laboratory Testing of Chemicals for Endocrine Disruption Potential - Analysis of Market Factors"(October 14, 2009) (Ref. 8). Shared costs in columns 3 & 4 of that table were divided by the tests included in the group, whichinclude analytical chemistry where appropriate.(a-2) The estimated costs for these assays were adjusted using an inflation factor to convert 2009$ to 2012$. Specifically, the estimated cost in (a-1) was entered into the onlineinflation calculator provided by US Department of Labor, Bureau of Labor and Statistics atEstimated CostsAs of 07/30/2012Page 1 of 1