### PMN Page 1 ; Page 1 of 40

FormA 10-31-96. 

### U.S. ENVIRONMENTAL PROTECTION AGENCY 

# ftE PA
PREMANUFACTURE

# ~ NOTTC'F.

When completed send th1s lorm to

# 11111111111111111111111111111111111~11111111111111111111111111111111111111 5 1 1 0 0 0 0 0 0 0 5 I S

Enter the total number of pages in the Premanufacture Notice 

# I 

# \'1 Date of receipt

··. ·:· :.;-··

-·· '. f \**~** .. J 

# 39 

# Company 

# San 

# ·r·.

.. IIZed

# ~llnmootros EPA case number 

# P-10·05

GENERAL INSTRUCTIONS 

## TS

• You must provide all information requested in this form to the extent that it is known to or reasonably ascertainable by you. Make reasonable estimates if you do
not have actual data.

• Before you complete this form, you should read the "Instructions Manual for Premanufacture Notification" (the Instructions Manual is available from the Toxic Substances Control Act (TSCA) Information Service by calling 202-554-1404, or faxing 202-554-5603). If a user fee has been remitted for this notice (40 CFR 700.45), indicate in the boxes above the TS-user fee identification number you have generated. Remember, your user fee ID number must also appear on your corresponding fee remittance, which is sent to EPA, Washington Financial Management Center (3303), P.O. 360399M, Pittsburgh, PA 15251-6399, Attn. TSCA User fee.

Part I- GENERAL INFORMATION

You must provide the currently correct Chemical Abstracts (CA) Name of the new chemical substance, even if you claim the identity as confidential. Yon may authorize another person to submit chemical identity information for you, but your submission will not be complete and the review will not begin until EPA receives this information. A letter in support of your submission should reference your TS user fee identification number. You must submit an original and two copies of this notice including all test data. If you claimed any information as confidential, a single sanitized copy must also be submitted.

Part II- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE

If there are several manufacture, processing, or use operations to be described in Part II, sections A and B of this notice, reproduce the sections as needed.

Partiii-LISTOF ATTACHMENTS

Attach additional sheets ifthere is not enough space to answer a question fully. Label each continuation sheet with the corresponding section heading. In Part Ill, list these attachments, any test data or other data and any optional information included in the notice.

OPTIONAL INFORMATION

You may include any information that you want EPA to consider in evaluating the new substance. On page II of this form, space has been provided for you to describe pollution prevention and recycling information you may have regarding the new substance.

So-called "binding" boxes are included throughout this form for you to indicate your willingness to be bound to certain statements you make in this section, such as use, production volume, protective equipment

. . . This option is intended to reduce delays that routinely accompany the development of consent orders or Significant New Use Rules. Except in the case of exemption applications (such as TMEA, L VE, LOREX) where certain information provided in such notification is binding on the submitter when the Agency approves the exemption application, checking a binding box in this notice does not by itself prohibit the submitter from later deviating from the information (except chemical identity) reported in the form.

CONFIDENTIALITY CLAIMS

You may claim any information in this notice as confidential. To assert a claim on the form, mark (X) the confidential box next to the information that you claim as confidentiaL To assert a claim in an attachment, circle or bracket the information you claim as confidential. If you claim information in the notices as confidential you must also provide

a sanitized version of the notice (including attachments). For additional instructions on claiming information as confidential, read the Instructions Manual.

Mark (x) if any information in this notice is claimed as confidentiaL

EPA FORM 7710-25 (Rev. 5-95) TEST DATA AND OTHER DATA 

# P-10-03

You are required to submit all test data in your possession or control and to provide a description of all other data known to or reasonably ascertainable by you, if these data are related to the health and environmental effects on the manufacture, processing, distribution in commerce, use, or disposal of the new chemical substance. Standard literature citations may be submitted for data in the open scientific literature. Complete test data (written in English) not summaries of data. must be submitted if they do not appear in the open literature. You should clearly identify whether test data is on the substance or on an analog. Also, the chemical composition of the tested material should be characterized. Following

are examples oftest data and other data. Data should be submitted according to the requirements of §720.50 of the Premanufacture Notification Rule (40 CFR Part 720).

Test Data (Check Below any included in this notice)

Environmental fate data 

# D Yes

Health effects data 

# D Yes

Environmental effects data 

# 0 Yes
Physical/Chemical Properties* 

# 0 Yes • Other data 

# D Yes

rusk assessments 

# p

Structure/activity**r~]-ationships(KI**
Test data not in the-possession

or control of the submitter 

# 0

A physical and chemical properties worksheet is located on the last page of this form.

1

.. _.1 TYPE OF NOTICE (Check Only One)

PMN (Prernanufacture Notice) 

# 0 

# D D 

# D D 

# D D INTERMEDIATE PMN (submitted in sequence with final produciPMN)

SNUN (Significant New Use Notice)

TMEA (Test Marketing Exemption Application)

LYE (Low Volume Exemption)@ 40 CFR 723.50(c)(l)

LOREX (Low Release/Low Exposure Exemption)@ 40 CFR 723.50(c)(2)

L VE Modification

IS THIS A CONSOLIDATED PMN? 

# D D LOREX Modification

Yes

### PMN Page 2 ; Page 2 of 40
Public reporting burden for this collection of information is estimated to average I I 

### 0 hours per response,**includi~Rg~ ~b!Q~p~i~**  

## 164 instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of
information. Send comments regarding the burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Director, Collection Strategies Division (2822), U.S. Environmental Protection Agency, 1200 Pennsylvania Ave., N.W., Washington, D.C. 20460; and to the Office of Management and Budget, Paperwork Reduction Act (2070-0012), Washington, D.C. 20503. 

## CERTIFICATION

-- A Printed copy 

### of this signature page, with original signature, must be submitted



### I certifY that to the best of my knowledge and belief:



### I. The company named in Part I, section A, subsection la of this notice form intends to manufacture or import for a commercial purpose, other than in small quantities solely for research and development, the substance identified in Part I, Section B.

2. All information provided in this notice is complete and truthful as ofthe date of submission.

3. I am submitting with this notice all test data in my possession or control and a description of all other data known to or reasonably ascertainable by me as required by §720.50 of the Premanufacture Notification Rule.

Additional Certification Statements:

If you are submitting a PMN, Intermediate PMN, Consolidated PMN, or SNUN, check the following user fee certification statement that applies: 

# D The Company named in Part I, Section A has remitted the fee of$2500 specified in 40 CFR 700.45(b), or 

# D The Company named in Part I, Section A has remitted the fee of$1000 for an Intermediate PMN (defined@ 40 CFR 700.43) in accordance with 40 CFR 700.45(b), or 

# 0 The Company named in Part I Section A is a small business concern under 40 CFR 700.43 and has remitted a fee of$100 in accordance with 40 CFR 700.45(b).

If you are submitting a low volume exemption (LVE) application in accordance with 40 CFR 723.50(c)(l) or a ),.ow release and low exposure exemption (LoRex) application in accordance with 40 CFR 723.50(c)(2), check the following certification statements: 

# D The manufacturer submitting this notice intends to manufacture or import the new chemical substance for commercial purposes, other than in small quantities solely for research and development, under the terms of 40 CFR 723.50. 

# 0 The manufacturer is familiar with the terms of this section and will comply with those terms; and 

# 0 The new chemical substance for which the notice is submitted meets all applicable exemption conditions. 

# D Ifthis application is for an LVE in accordance with 40 CFR 723.50(c)(l), the manufacturer intends to commence manufacture of the exempted substance for commercial purposes within I year of the date of the expiration of the 30 day review period.·

The accuracy of the statements you make in this notice should reflect your best prediction of the anticipated facts regarding the chemical substance described herein. Any knowin!l and willful misinterpretation is subject to criminal penalty pursuant to 18 USC 1001. Signature and**t~** of Anthony Official (Ori!!inal Signature Required) 

# Prll.t 1 

# -dl., "/- Date

# ~/ 

# 7/D. rCL -""/ -f! CEO 

# /tJ -1- 

# zoo?**Signature~** (if applicable) Date

EPA FORM 7710-25 (Rev. 5-95) 

### Page 2 Confidential 

# D DConfidential Ia. Person Submitting Notice (in U.S.)

# --------------~-------------[]

b. Agent (if applicable)

### f3ll¥h~~st NanoTechnologies, Inc.

Mailing address. (number and street) 2501 Tecnnology Place

City, State Norman, OK

Name of authorized official

Company

Mailing address (number and street)

City, State 

### 73071

Postal Code 

### Telephone (include area code)

c. If you are submitting this notice as part of a joint submission, mark (X) this**box·----------------------1~**
Joint Submitter (if applicable)

2. Technical Contact (in U.S.) Name of authorized official

Company

Mailing address (number and street)

Province, Country

Name. of**authQTi;~:ed** official PhilJ.p wallJ.s

Comoanv

. Soucnwest NanoTechnologJ.es, Inc.

M ai I ing addressi( number 1 and street) 2501 -recrino ogy P ace

City, State Norman, OK

3. If you have had a prenotice communication (PC) concerning this notice and Code

Postal Code 

### 73071

EPA assigned a PC Number to the notice, enterth**::;:::,e.,::num=b~e::.,;r.** ____ 

### -+

4.

5.

6. If you previously submitted an exemption application for chemical substance covered by this notice, enter the exemption number assigned by EPA. If you previously submitted a PMN for this substance enter the PMN number EPA withdrawn or If you for the chemical substance covered by this notice, enter the notice number assigned by EPA.

Type of Notice- Mark (X) I. 

# 0 D Manufacture Only Binding Option Mark

EPA FORM 7710-25 (Rev. 5-95) 

### X-09-0003

I 2. I I I 

# D D

Page 3 , State



### Telephone (include country or area code)

acturing & Technical Support



### Telephone (include area code)
405-217-8388

Import Only Mark (X) if none

Mark(X) if none

Mark(X) if none

Binding Option Mark I I 3. I I I 

# D Both 

# D[]

## PMN Page 4 ; Page 4 of 40 Page 000004 of 117 SAN

Part I

-- GENERAL INFORMATION-- Continued Section B --CHEMICAL IDENTITY INFORMATION: 

# I You must provide a currently correct Chemical Abstracts (CA) name of the substance based on the ninth Collective Index (9Cl) ofCA nomenclature rules and conventions. Mark (X) the "Confidential" box next to any item you claim as confidential Complete either item I (Class I or 2 substances) or 2 (Polymers) as appropriate. Complete all other items.

If another person will submit chemical identity information for you (for either Item I or 2), mark (X) the box at the right. 

# D Identify the name, company, and address of that person in a continuation sheet. I. Class I or 2 chemical substances (for**definition~** class I and class 2**substan~see** the Instructions Manual)

a. Class of substance

- Mark (X)**~** Class I or 

### I.,J Class 2 b. Chemical name (Currently correct Chemical Abstracts (CA) Name that is consistent with TSCA Inventory listings for similar substances. For Class I substances a CA Index Name must be provided. For Class 2 substances either a CA Index Name orCA Preferred Name must be provided, which ever is appropriate based on CA 9CI nomenclature rules and conventions). 

### Single-wall carbon nanotube



### c c. Please identify which method you used to develop or obtain the specified chemical identity information reported in this notice: (check one). 

# D

... Method I (CAS Inventory Expert Service- a copy of the Identification 

# r;J Method 2 (Other Source) report obtamed from the CAS Inventory Expert Servtces must be 

## t.::J submitted as an attachment to this notice) d. Molecular formula 

### CBI 

# D CAS Registry Number (if a number already exists for the substance)

e. For a class I substance, provide a complete and correct chemical structure diagram. For a class 2 substance, provide a correct representative or partial chemical structure diagram, as complete as can be known, if one can be reasonably ascertained. Please see the E-PMN Instruction Manual for discussion of "native format" diagram software which can be helpful in reviewing your substance.



# SEE ATTACHMENT 10e STRUCTURE OF CARBONNANOTUBES



# D Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page4 Confidential 

# D D



# D D

## Page 000005 of 117 SAN

PMN Page 4a ; Page 5 of 40

For a class 2 substance- (1) List the immediate precursor substances with their respective CAS Registry Numbers. (2) Describe the nature of the reaction or process. (3) Indicate the range of composition and the typical composition (where appropriate).

e. (1) List the immediate precursor substances with their respective CAS Registry Numbers.

Name (CAS#)

e. (2) Describe the nature of the reaction or process.

e. (3) Indicate the range of composition and the typical composition (where appropriate).



# 0_ Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 4a 

### Confi

### dential 

# D

D



# D

### PMN Page 5 ; Page 6 of 40 

### Page 000006 of 117 SAN

Part I-- GENERAL INFORMATION-- Continued Section B

-- CHEMICAL IDENTITY INFORMATION-- Continued 2. Polymers (For a definition of polymer, see the Instructions Manual.) Contidential

a. Indicate the number-average weight of the lowest molecular weight composition of the polymer you intend to manufacture. 

# D Indicate maximum weight percent oflow molecular weight species (not including residual monomers, reactants, or solvents) below 500 and below I ,000 absolute molecular weight of that composition. Describe the methods of measurement or the basis for your estimates: GPCU

# Oth~ 

# r'

(Spodfy

# bol~) (i) lowest number average molecular weight: 

# I (ii) maximum weight% below 500 molecular weight:
(iii) maximum weight % below I 000 molecular weight:


# 0 Mark (X) this box if you attach a continuation sheet. b. You must make separate confidentiality claims for monomer or other reactant identity, composition information, and residual information. Mark (X) the "Confidential" box next to any item you claim as confidential (1)



### .. Provide the specific chemical name and CAS Registry Number (if a number exists) of each monomer or other reactant used in the manufacture of the polymer. (2)



# - Mark (X) this column if entry in column (1) is confidential. (3)



# - Indicate the typical weight percent of each monomer or other reactant in the polymer. (4)



# - Choose "yes" from drop down menu if you want a monomer or other reactant used at two weight percent or less to be listed as part of the polymer description on the TSCA Chemical Substance Inventory. (5)



# - Mark (X) this column if entries in columns (3) and (4) are· confidential. (6)



# - Indicate the maximum weight percent of each monomer or other reactant that may be present as a residual in the polymer as manufactured for commercial purposes. (7)



# - Mark (X) this column if entry in column (6) is confidential. Monomer or other reactant and CAS Registry Number Conti- Typical Include in Conti- Maximum Contidential composition identity dential residual dential (I) 

# <n ill _ill_ (5) (6) (7) 

# D % 

# D 

# D % 

# D D % 

# D 

# D % 

# D 

# D % 

# D 

# D % 

# D D %



### .... .. 11 

# D % 

# D D % 

# D % 

# D 

# D

%1C 

# D % 

# D 

# D % 

# D D % 

# D CJ %1 

# lc=J %

. ···i 

# CJ % 

# [ 

# lc=J % 

# CJ % 

# L 

# lc=J % 

# CJ % 

# I**~lc=J** % 

# CJ % 

# I Jc=J % 

# I 

# c=J % 

# I 

# ]c=J % 

# I 

# I D Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 5c.

d.

e. 

## PMN Page 5a; 

## Page 7 of 40 

## Paae 000007 of 117 SAN
P. !.ease identify which method you used to develop or obtain the specified chemical identityO.·.n

.. £.ormation reported in this notice (check one). 

# D Method I (CAS Inventory Expert Service- a copy of the identification report

... : Method 2 (other source) obtained from CAS Inventory Expert Service must be submitted as an attachment to this notice)

The currently correct Chemical Abstracts (CA) name for the polymer that is consistent with TSCA Inventory listings for similar polymers.

CAS Registry Number (if a number already exists for the substance)

Provide a correct representative or partial chemical structure diagram, as complete as can be known, if one can be reasonably ascertained.

. Please
see the E-PMN Instruction Manual for discussion of "native format" diagram software which can be helpful in reviewing your substance.



# L ]Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page Sa 

### CBI 

# D D

D D3. Impurities (a)

- Identify each impurity that may be reasonably anticipated to be present in the chemical substance as manufactured for commercial purpose. Provide the CAS Registry Number if available. If there are unidentified impurities, enter "unidentified." Estimate the maximum % of each · If there are unidentified estimate their total %. Impurity and CAS Registry Number Maximum Confipercent dential

Synonyms

- Enter any chemical synonyms for the new chemical identified in subsection I or 2. 

### None


# 0 Mark (X) this box if you attach a continuation sheet.
5. -List



# 0 Mark (X) this box if you attach a continuation sheet.

6. Generic chemical name- If you claim chemical identify as confidential, you must provide a generic name for your substance that reveals the specific chemical identity of the new chemical substance to the maximum extent possible. Refer to the TSCA Chemical Substance Inventory, 1985 Edition, Appendix B for guidance on developing generic names. %

%

%

%

%

%

new chemical substance. Provide the CAS

CAS Number

EPA FORM 7710-25 (Rev. 5-95) Page6 

# w w w w D D D

Confidential 

# D

### PMN Page 7 ; Page 9 of 40 n. nnnnnn ,.r;**~ ~**  "7 

### c A r-1 

## Part I-- GENERAL INFORMATION-- Contmuea Section C --PRODUCTION, IMPORT, AND USE INFORMATION:
Mark (X) the "Confidential" box next to any item you claim as confidential. 

### 1. 

### Production volume-- Estimate the 

### maximum production volume during the first 12 months of production. Also estimate the maximum production volume for any consecutive 12-month period during the first three years of production. Estimates should be on 100% new chemical substance basis. For a Low Volume Exemption application, if you choose to have your notice reviewed at a lower production volume than 10,000 l<Jjyr, specify the volume and mark_(x) in the binding box. lfgranted,_you are bound to this volume Maximum first 12-month production (kg/yr) Maximum 12-month production (kg/yr) Confi- (100% new chemical substance basis) (100% new chemical substance basis) dentlal Binding Option Mark (x)

2. Use Information

-- You must make separate confidentiality claims for the description of the category of use, the percent of production volume devoted to each category, the formulation of the new substance, and other use information. Mark (X) the "Confidential" Box next to any item you claim as confidential.

a. (1) --Describe each intended category of use of the new chemical substance by function and application. (2) --Mark (X) this column if entry column (1) is confidential business information (CBI). (3) --Indicate your willingness to have the information provided in column (1) binding. (4) --Estimate the percent of total production for the first three years devoted to each category of use. (5) --Mark (X) this column if entry in column (4) is confidential business information (CBI). (6)

-- Estimate the percent of the new substance as formulated in mixtures, suspensions, emulsions, solutions, or gels as manufactured for commercial purposes at sites under

our control associated with each category of use. (7) --Mark (X) this column if entry in column (6) is confidential business information (CBI). (8) --Indicate% of product volume expected for the listed "use" sectors. Mark more than one box if appropriate. Mark (X) to indicate your willingness to have the use type provided in (8) binding. (9) --Mark (X) this column if entry(ies) in column (8) is (are) confidential business information (CBI). Category of use (I) (by function and application i.e. a CBI Binding Prod- CBI %in CBI %of substance expected per use CBI dispersive dye for finishing polyester fibers)

### ~~~n uc;,.on

### ~~~':: (8)



### Conductive coating using SWNT dispersion or SWNT ink. (see PMN 

### pg 10, pg lOa and Attachment)
Additive(SWNT powder)in resins/thermoplastics/elastomers for mechanical reinforcement. (see Attachments)
Manufacture of sheet composites (Buckypaper) using SWNT (no process information is available) (2) 

# D D D D D D D (x) (3) 

# D D D D D D D (4)
%

%

%

%

%

%

% (5) (6)

# ~ %

# ~ %

# ~ % 

# D % 

# D % 

# D % 

# D % (7)

# ~**~ ~**  

# D D D D**Site~** limited Consumer Industrial



### 100

100

100 Commercia! Binding Option 

# D D D D D D D (9) 

# D D D D D D D

* If you have identified a "consumer" use, please provide on a continuation sheet a detailed description of the use(s) of this chemical substance in consumer products.

In addition include estimates of the concentration of the new chemical substance as expected in consumer products and describe the chemical reactions by which this riubstance loses its identity in the consumer product. LJMark (X) this box if you attach a continuation sheet. b. Generic use If you claim any category of use description in subsection 2a as confidential, enter a generic description of that category. Read the Instruction description Manual for examples of generic use descriptions.

Mark (X) this box if you attach a continuation sheet.
3. Hazard Information

-- Include in the notice a copy of reasonable facsimile of any hazard warning statement, label, material safety data sheet, or other

information which will be provided to any person who is reasonably likely to be exposed to this substance regarding protective equipment or practices for the safe handing, transport, use, or disposal of the new substance. List in part III hazard information you include.

Mark (X) this box if you attach hazard information.

EPA FORM 7710-25 (Rev. 5-95) Page 7 Binding Option Mark(x)

### Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER box next to any item you claim Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are further industrial · or use after You must See manual

of the site at which the will occur. Name 

### SouthWest NanoTechnologies, Inc.

Site address (number and street) 

### 2501 Technology Place

ZIP code 

### Cleveland

If the same operation will occur at more than one site, enter the number of sites. Identify the additional sites on a continuation sheet, and if any ofthe sites have significantly different production rates or operations, include ali the information requested in this section for those sites

I. Batch

2. Continuous Maximum kg/day (100% new chemical Hours/day substance)

d. Process description Mark (X) to indicate your willingness to have your process description binding. 73071 dential 

# D



# D

D

(!) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon drum, rail car, tank truck, etc.). (2) Provide the identity, the approximate weight (by kg/day or kg/batch on a I 00% new chemical substance basis), and entry point of all starting materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch.). (3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media at the same step, assign a second release number for the second medium.

attach a sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 8

### PMN Page 

### Sa ; Page 11 of 40 

## Page 000011 of 117 SAN

Confidential



# CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL

EPA FORM 7710-25 (Rev. 5-95) Page 8a

### Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER
Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are further industrial or use You must See manual

of the site at which the will occur.

Site address (number and street)

City, County, State, ZIP code

If the same operation will occur at more than one site, enter the number of sites. Identify the additional sites on a continuation sheet, and if any of the sites have significantly different production rates or operations, include all the information requested in this section for those sites as

1. Batch

2. Continuous Hours/batch

Maximum kg/day (100% new chemical Hours/day substance)

d. Process description Mark (X) to indicate your willingness to have your process description binding. Use

Batches/year

Days/year dential 

# D



# D

D

(I) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specif'y- e.g. 5 gallon pails, 55 gallon drum, rail car, tank truck, etc.). (2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch.). (3) IdentifY by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media at the same step, assign a second release number for the second medium.

attach a continuation sheet.
EPAFORM7710-25 (Rev. 5-95) Page 8 -extra-1

### PMN Page 

### Sa ; Page 13 of 40 

### Page 000013 of 117 SAN

Confidential



# CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL

EPA FORM 7710-25 (Rev. 5-95) Page 8a -extra-1box next to any item you claim Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are further industrial or use after You must describe these · See instructions manual 1. which the will occur.

Site address (number and street)

City, County, State, ZIP code

If the same operation will occur at more than one site, enter the number of sites. Identify the additional sites on a continuation sheet, and if any of the sites have significantly different production rates or operations, include all the information requested in this section for those sites as attachments.

I. Batch

2. Continuous Maximum kg/day (100%newchemical Hours/day substance) Use

Batches/year

Days/year Confidential 

# D

D



# D

d. Process description Mark (X) to indicate your willingness to have your process description binding. (!) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon drum, rail car, tank truck, etc.). (2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch.). (3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media at the same step, assign a second release number for the second medium.

attach a continuation sheet.
EPAFORM7710-25 (Rev. 5-95) Page 8 -extra-2

### PMN Page 

### Sa ; Page 15 of 40 

## Page 000015 of 117 SAN

Confidential



# CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-2

### Section 

### A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER Mark (X) the "Confidential" box next to any item you claim as confidential Complete section 

### A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are or use after See 

### 1.

Site address (number and street)

City, County, State, ZIP code

If the same operation will occur at more than one site, enter the number of sites. Identify the additional sites on a continuation sheet, and if any of the sites have significantly different production rates or operations, include all the information requested 

### in this section for those sites as attachments.

I. Batch

2. Continuous Maximum kg/day (100%newchemical Hours/day substance) Use

Batches/year Confidential 

# D

D

D

d. Process description Mark (X) to indicate your willingness to have your process description binding. (I) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon drum, rail car, tank truck, etc.). (2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch.). (3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media at the same step, assign a second release number for the second medium.

attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 8 -extra-3

### PMN Page 

### Sa ; Page 17 of 40 

## Page 000017 of 117 SAN

Confidential



# CONFIDENTIAL CONFIDENTIAL

. CONFIDENTIAL

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-3

### Section A-- 

### INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER Mark (X) the "Confidential" box next to any item you claim as confidential Complete section 

### A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting requirements if there are after You must describe these See instructions manual

Site address (number and street)

City, County, State, ZIP code

If the same operation will occur at more than one site, enter the number of sites. Identify the additional sites on a continuation sheet, and if any of the sites have significantly different production rates or operations, include all the information requested in this section for those sites as

Hours/batch

I. Batch

2. Continuous
d. Process description Mark (X) to indicate your willingness to have your process description binding. dential 

# D

D

D

( 1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specifY- e.g. 5 gallon pails, 55 gallon drum, rail car, tank truck, etc.). (2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch.). (3) Identity by number the points of release, including small or intermittent releases, to tbe environment of the new chemical substance. If releasing to two media at the same step, assign a second release number for the second medium.

attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 8 -extra-4

### PMN Page 

### Sa ; Page 19 of 40 

## Page 000019 of 117 SAN

Confidential



# CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-4box next to any item you
Complete section A for each type**ofm<onnt~r·tm·<>** control. Importers do not have to complete this there are further industrial operation involving the new chemical substance at industrial sites you outside the U.S.; however, you may still have reporting requirements if

ou must describe these See instructions manual 

### 1. which the will occur.

Site address (number and street)

City, County, State, ZIP code

If the same operation will occur at more than one site, enter the number of sites. Identify the additional sites on a continuation sheet, and if any of the sites have significantly different production rates or operations, include all the information requested in this section for those sites as attachments.

1. Batch

2. Continuous Hours/batch

Maximum kg/day (lOO%newchemical Hours/day substance) Use

Batches/year

Days/year Confi· dential 

# D

D D D

d. Process description Mark (X) to indicate your willingness to have your process description binding. (I) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specif'y- e.g. 5 gallon pails, 55 gallon drum, rail car, tank truck, etc.). (2) Provide the identity
, the approximate weight (by kg/day or kg/batch on a I 00% new chemical substance basis), and entry point of all'starting materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch.). (3) IdentifY by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If releasing to two media at the same step, assign a second release number for the second medium.

attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 8 -extra-5

### PMN Page 

### Sa ; Page 21 of 40 

## Page 000021 of 117 SAN

Confidential



# CONFIDENTIAL CONFIDENTIAL CONFIDENTIAL

EPA FORM 7710-25 (Rev. 5-95) Page Sa -extra-5

### Page 000022 of 117 SAN PMN Page 9 ; Page 22 of 40

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. (I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. (2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). (3) --Describe any protective equipment and engineering controls used to protect workers. (4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. (5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the time of exposure. (7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). (8) --Estimate the maximum number of workers involved in each activity for all sites combined. (9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). (I 0) and (II)

-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. ( 12)

-- Mark (X) this column if entries in columns (I 0) and (II) are confidential business information {CBI). Worker activity CBI Protective Equipment/ Binding Physical Binding forms(s) CBI #of CBI Maximum duration CBI
(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr Mark(x) substance Mark(x) Exposed (I) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) 

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EPA FORM 7710-25 (Rev. 5-95) Page 

### 9

## Page 000023 of 117 SAN PMN Page 9a; Page 23 of 

## 40

3. Environmental Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. (I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3 ). (2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). (3) --Mark (X) this column if entries in columns (I) and (2) are confidential business information (CBD. ( 4) --IdentifY the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specifY)) to which the new substance will be released from that release point. (5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). (6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). (7) --IdentifY the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBI). Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI Number substance released release efficiency data)

(!) (2a) (2b) e.g. stack air Binding (3) (4) (Sa) Mark (Sb) (6) (X)

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# D See attached "Additional

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### (7) Mark (X) the destination(s) of releases to water. 

### NPDES# CBI

## ~ POTW--provide 

### Non-contact cooling water to City of .Norman. OPDES 0029190 name(s) 

# D
D Navigable waterway-- 

# D provide name(s) 

# D Other--SpecifY 

# D

] Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9a

## Page 000024 of 117 SAN PMN Page 9 ; Page 24 of 40

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 2. Occupational Exposure --You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. (1) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. (2) --Mark (X) this column if entry in column (1) is confidential business information (CBI). (3) --Describe any protective equipment and engineering controls used to protect workers. (4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. (5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the time of exposure. (7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). (8) --Estimate the maximum number of workers involved in each activity for all sites combined. (9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). ( 1

0) and ( 11)

-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. _i12)

-- Mark_fX) this column if entries in columns (10) and (11) are confidential business information (CBI). Worker activity CBI Protective Equipment/ Binding Physical Binding forms(s) CBI #of CBI Maximum duration CBI
(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr Mark(x) substance Mark(x) Exposed (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (II) (12) 

# B 

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# D D B ....---- 

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# 8 

# D D B " 

# 8

1 Mark (X) this box if you attach a continuation sheet.

EPA FORM 77!0-25 (Rev. 5-95) Page 9 -extra-1

### Page 000025 of 117 SAN PMN Page 9a ; 

### Page 25 of 40

3. Environmental Release and Disposal

-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance released and other release and disposal infonnation. Mark (X) the "Confidential" box next to each item you claim as confidential. (I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3). (2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). (3) --Mark (X) this column if entries in columns (I) and (2) are confidential business infonnation (CBI). (4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to which the new substance will be released from that release point. (5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). (6) --Mark (X) this column if entries in columns (4) and (5) are confidential business infonnation (CBI). (7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business infonnation (CBI). Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI Number substance released release efficiency data)

(I) (2a) (2b) e.g. stack air Binding (3) (4) (Sa) Mark (Sb) (6) _ffi 

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### City of Norman, OK OPDES 0029190 name(s) 

# D

D Navigable waterway-- 

# D provide name(s) 

# D Other--Specify 

# D
D Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-1

## Page 000026 of 117 SAN PMN Page 9 ; Page 26 of 40

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 2. Occupational Exposure --You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim 

### as confidential. (I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. (2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). (3) --Describe any protective equipment and engineering controls used to protect workers. (4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. (5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the time of exposure. (7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). (8) --Estimate the maximum number of workers involved in each activity for all sites combined. (9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). (I 0) and (II)

-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. (12) --Mark (X) this column if entries in columns (10) and (II) are confidential business information (CBI). Worker activity CBI Protective Equipment/ Binding Physical Binding forms(s) CBI #of CBI Maximum duration CBI
(i.e., bag dumping, filling drums) Engineering Controls Option and %new Option Workers Hrs/day Days/yr Mark(x} substance Mark(x) Exposed (!) (2) (3) (4) (5) (6) (7) (8) (9) (!0) (ll) (12)

# ~ 

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EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-2

## Page 000027 of 117 SAN PMN Page 

### 9a ; Page 27 of 40

3. Environme11tal Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. (I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3). (2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). (3) --Mark (X) this column if entries in columns (I) and (2) are confidential business information (CBI). (4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to which the new substance will be released from that release point. (5) --a. Describe control technology, if any, and control efficiency that will be. used to limit the release of the new substance to the environment. For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). (6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). (7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBD. Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI Number substance released release efficiency data)

(!) (2a) (2b) e.g. stack air Binding (4) Mark (3) (Sa) (X) (Sb) (6)

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(7) Mark (X) the destination(s) of releases to water. 

### NPDES# CBI

## ~ POTW --provide 

### City of Norman, OK OPDES 0029190 name(s) 

# D

D Navigable waterway-- 

# D provide name( s) 

# D Other--Specify 

# D U Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-2

### Page 000028 of 117 SAN PMN Page 9 ; Page 28 of 40

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. (I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. (2) --Mark (X) this column if entry in column (I) is confidential business information (CBD. (3) --Describe any protective equipment and engineering controls used to protect workers. (4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. (5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the time of exposure. (7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). (8) --Estimate the maximum number of workers involved in each activity for all sites combined. (9) --Mark (X) this column if entry in column (8) is confidential business information (CBD. (I 0) and ( 11)

-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. (12) --Mark (X) this column if entries in columns (10) and (11) are confidential business information {CBI). Worker activity CBI Protective Equipment/ Binding Physical Binding fonns(s) CBI #of CBI Maximum duration CBI
(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr Mark(x) substance Mark(x) Exposed (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) 

# B 

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EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-3

## Page 000029 of 117 SAN PMN 

### Page 9a ; Page 29 of 40

3. Environmental Release and Disposal

-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. (I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3 ). (2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg' day or kg'batch). (3) --Mark (X) this column if entries in columns (I) and (2) are confidential business information (CBI). ( 4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to which the new substance will be released from that release point. ( 5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg'day). (6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). (7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBI). Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI Number substance released release efficiency data)

(I) (2a) (2b) e.g. stack air Binding (4) Mark (3) (5a) IX) (5b) (6)

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(7) Mark (X) the destination(s) of releases to water. 

### NPDES# CBI

## ~ POTW --provide 

### City of Norman, OK OPDES 0029190 name(s) 

# D

D Navigable waterway-- 

# D provide name(s) 

# D Other--Specify 

# D
D Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-3

## Page 000030 of 117 SAN PMN Page 9 ; Page 30 of 40

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. (I) --Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. (2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). (3) --Describe any protective equipment and engineering controls used to protect workers. (4) and (6) --Indicate your willingness to have the information provided in column (3) or (5) binding. (5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the. time of exposure. (7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). (8) --Estimate the maximum number of workers involved in each activity for all sites combined. (9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). (10) and (II)-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. (12) --Mark (X) this column if entries in columns (10) and (II) are confidential business information (CBI). Worker activity CBI Protective Equipment/ Binding Physical Binding fonns(s) CBI #of CBI Maximum duration CBI
(i.e., bag dumping, filling drums) Engineering Controls Option and o/onew Option Workers Hrs/day Days/yr Mark(x) substance Mark(x) Exposed (I) (2) (3) (4) (5) (6) (7) (8) (9) (10) (II) (12) 

# B 

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EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-4

## Page 000031 of 117 SAN PMN Page 

### 9a ; Page 31 of 40

3. Environmental Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. ( 1) --Enter the number of each release point identified in the process description, part II, section A, subsection 1 d(3 ). (2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). (3) --Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). ( 4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to which the new substance will be released from that release point. (5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). (6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). (7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark(X) if the POTW name or NPDES #is confidential business information (CBI). Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI Number substance released release efficiency data)

(!) (2a) (2b) e.g. stack air Binding (4) Mark (3) (Sa) (X) (Sb) (6)

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(7) Mark (X) the destination(s) of releases to water. 

### NPDES# CBI

## ~ POTW --provide 

### City of Norman, OK OPDES 0029190 name(s) 

# D

D Navigable waterway-- 

# D provide name(s) 

# D Other--Specify 

# D

] Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-4

## Page 000032 of 117 SAN PMN Page 9 ; Page 32 of 40

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section A-- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER-- Continued 2. Occupational Exposure-- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical substance, number of works exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. (!)--Describe the activities (i.e. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the substance. (2) --Mark (X) this column if entry in column (I) is confidential business information (CBI). (3) --Describe any protective equipment and engineering controls used to protect workers. ( 4) and ( 6)

-- Indicate your willingness to have the information provided in column (3) or ( 5) binding. (5) --Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and% new chemical substance (if part of a mixture) at the time of exposure. (7) --Mark (X) this column if entry in column (5) is confidential business information (CBI). (8) --Estimate the maximum number of workers involved in each activity for all sites combined. (9) --Mark (X) this column if entry in column (8) is confidential business information (CBI). (10) and (II)-- Estimate the maximum duration of the activity for any worker in hours per day and days per year. (12) --Mark (X) this column if entries in columns (10) and (II) are confidential business information (CBI). Worker activity CBI Protective Equipment/ Binding Physical Binding forms(s) CBI #of CBI Maximum duration CBI
(i.e., bag dumping, filling drums) Engineering Controls Option and%new Option Workers Hrs/day Days/yr Mark(x) substance Mark(x) Exposed (I) (2) (3) (4) (5) (6) (7) (8) (9) (10) (ll) (12) 

# B D 

# D B D 

# 8 D 

# 8

9 

# B**~** 

# B 

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9 

# 8 

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# B

9 

# B 

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# B 

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9 

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# "

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D Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9 -extra-5

## Page 000033 of 117 SAN PMN 

### Page 9a ; Page 33 of 40

3. Environmental Release and Disposal-- You must make separate confidentiality claims for the release number and the amount of the new chemical substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. (I) --Enter the number of each release point identified in the process description, part II, section A, subsection I d(3 ). (2) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). (3) --Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). (4) --Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify)) to which the new substance will be released from that release point. (5) --a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). (6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). (7) --Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES #is confidential business information (CBI). Release Amount of new CBI Mediwnof Control technology and efficiency (you may wish to optionally attach CBI Number substance released release efficiency data)

(1) (2a) (2b) e.g. stack air Binding (3) (4)

. (Sa) Mark (Sb) (6) (X)

# ~ 

# D**~**

# ~ 

# D**~**

# ~ 

# D**~**

# ~ 

# D**~**

# ~ 

# D**~**

# ~ 

# D**~**

# ~ 

# D**~** 

# D 

# D D 

# D 

# D D

(7) Mark (X) the destination(s) of releases to water. NPDES# 

## CBl 

# [!] POTW--provide 

### City of Norman, OK OPDES 0029190 name(s) 

# D

0 Navigable waterway-- 

# D provide name(s) 

# D Other--Specify 

# D
J Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 9a -extra-5

### PMN Page 10; Page 34 of 40 

## Page 000034 of 117 SAN

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE-- Continued Section B --INDUSTRIAL SITES CONTROLLED BY OTHERS Complete section B for typical processing or use operations involving the new chemical substance at sites you do not control. Importers do not have to complete this section for operations outside the U.S.; however, you must report any processing or use activities after import. See the Instructions Manual. 

### Complete a separate section Bfor each type of processing, or use operation involving the new chemical substance. If the same operation is performed at more than one site describe the typical operation common to these sites. Identify additional sites on a continuation sheet. 

### l(a). Operation Description-- To claim infonnation 

### in this section as confidential, circle or bracket the specific infonnation that you claim as confidential. (I)-- Diagram the major unit operation steps and chemical conversions, including interim storage and transport containers (specify- e.g. 5 gallon pails, 55 gallon drums, rail cars, tank trucks, etc). On the diagram, identify by letter and briefly describe each worker activity. (2) --Either in the diagram or in the text field !(b) below, provide the identity, the approximate weight (by kg/day or kg/batch, on an 100% new chemical substance basis), and entry point of all feedstocks (including reactants, solvents and catalysts, etc) and all products, recycle streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch). (3) --Either in the diagram or in the text field I (b) below, identify by number the points of release, including small or intermittent releases. to the environment of the new chemical substance. ( 4) Please enter 

### CBI the# of sites (remember to identify the locations of these sites on a continuation sheet): --"1'-:--- # of sites



# CONFIDE}JTI.t\L Co i',Tf,**IDE·~-** 

## TTT**~** 

## T
..L**~** ..L "'J .L .J...L**~.L..J**

# COl'~FIDENTIAL

I (b). (Optional) This space is for a text description to clarify the diagram above.

] Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page 10

### PMN Page 1 Oa ; Page 35 of 40

2. Worker Exposure/Environmental Release 

## Page 000035 of 117 SAN (I) --From the diagram above, provide the letter for each worker activity. Complete 2-8 for each worker activity described. (2) --Estimate the number of workers exposed for all sites combined. (4) --Estimate the typical duration of exposure per worker in (a) hours per day and (b) days per year. ( 6) --Describe physical form of exposure and % new chemical substance (if in mixture), and any protective equipment and engineering controls, if any, used to protect workers. (7) --Estimate the percent of the new substance as formulated when packaged or used as a final product. (9)

-- From the process diagram above, enter the number of each release point. Complete 9-13 for each release point identified. (10) --Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology to the environment (in kg/day or kg/batch). ( 12) --Describe media of release i.e. stack air, fugitive air (optional-see Instructions Manual), surface water, on-site or off-site land or incineration, POTW, or other (specify) and control technology, if any, that will be used to limit the release of the new substance to the environment. ( 14)

-- Identify byproducts which may result from the operation. (3), (5), (8 :(II), 13) and (!5) --Mark (X) this column if any of the proceeding entries are confidential business information (CBI). Lette #of CBI Duration Of CBI 

### Protective Equip. /Engineering Controls/Physical Form and/% new substance %in CBI

of Workers Exposure FormActi- Exposed ulation vity (1) (2) (3) (4a) (4b) (5) (6) (7) (8)

# ~**~ ~** **~ ~** **~ ~** **~ ~** 

# ~**~ ~**  

# D D D

Release Amount of CBI 

### Media of Release & Control Technology CBI Number New Substance Released (9) _{lOa) lOb) 11 

### Iii\ (13) 

# [g) [g) [g) [g) [g) [g) [g) [g) [g) [g)

( 14) -- Byproducts: (15) 

# D D Mark (X) this box if you attach a continuation sheet,



# OPTIONAL POLLUTION PREVENTION INFORMATION To claim information in the following section as confidential circle or bracket the specific information that you claim as confidential. In this section you may provide information not reported elsewhere in this form regarding your efforts to reduce or minimize potential risks associated with activities surrounding manufacturing, processing, use and disposal of the PMN substance. Please include new information pertinent to pollution prevention, including source reduction, recycling activities and safer processes or products available due to the new chemical substance. Source reduction includes the reduction in the amount or toxicity of chemical wastes by technological modification, process and procedure modification, product reformulation, raw materials substitution, and/or inventory control. Recycling refers to the reclamation of useful chemical components from wastes that would otherwise be treated or released as air emissions or water discharges, or land disposal. Descriptions of pollution prevention, source reduction and recycling should emphasize potential risk reduction subsequent to compliance with existing regulatory requirements and can be either quantitative or qualitative. The EPA is interested in the information to
assess overall net reductions in toxicity or environmental releases and exposures, not the shifting of risks to other environmental media or nonenvironmental areas (e.g., occupational or consumer exposure). In addition, information on the relative cost or performance characteristics of the PMN substance to potential alternatives may be provided. All information provided in this section will be taken into consideration during the review of this substance. See PMN Instructions Manual and Pollution Prevention Guidance manual for e;uidance and examnles.

EPA FORM 7710-25 (Rev. 5-95) Page lOa

### PMN Page 11 ; 

### Page 36 of 40 

### Page 000036 of 117 SAN

Optional Pollution Prevention Information (Continued) Describe the expected net benefits, such as (1) an overall reduction in risk to human health or the environment; (2) a reduction in the volume manufactured; (3) a reduction in the generation of waste materials through recycling, source reduction or other means; (4) a reduction in potential toxicity or human exposure and/or environmental release; (5) an increase in product performance, a decrease in the cost of production and/or improved operation efficiency of the new chemical substance in comparison to existing chemical substances used in similar application; or 

### (6) the extent to which the new chemical substance may be a substitute for an existing substance that poses a greater overall risk to human health or the environment. CBI 

# D

[ ] Mark (X) this box if you attach a continuation sheet.

EPA FORM 7710-25 (Rev. 5-95) Page II

### PMN Page 12 ·Page 

## 37 of 40 

### ' 

## p 

## a_g_e 000037 

## f 117 SAN 0 

# Part III-- LIST OF ATTACHMENTS

Attach continuation sheets for sections of the form and test data and other data (including physical/chemical properties and structure/activity information), and optional information after this page. Clearly identify the attachment and the section of the form to which it relates, if appropriate. Number consecutively the pages of any paper attachments. In the column below, enter the inclusive page numbers of each attachment. Electronic attachments can be identified by filename. Mark (X) the "Confidential" box next to any attachment name you claim as confidential. Read the Instructions Manual for guidance on how to claim any information in an attachment as confidential. You must include with the sanitized copy of the notice form a sanitized version of any attachment in which you claim information as confidential. # Attachment name Attachment Filename Attachment Confipage number( s) dential


### 1

# ~ 2

# ~ 3

# ~ 4 10e Structure of Carbon Nanotubes 100-109 

# D

5 SouthWest NanoTechnologies MSDS 110-114 

# D

6 SouthWest NanoTechnologies Product Label 115 

# D

7

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# ~ 19

# ~ 20

# ~ 21

# ~ 22 Ohio EPA Hazardous Waste Facility Permit Renewal Letter 163-164 

# D

23 

# D

24 

# D

[ ] Mark (X) this box if you attach a continuation sheet. Enter the attachment name and number.

EPA FORM 7710-25 (Rev. 5-95) Page 12

### PMN Page 13 ; Page 38 of 40 

## p aae 000038 

## f 117 SAN 0 

### PHYSICAL AND CHEMICAL PROPERTIES WORKSHEET To assist EPA's review of physical and chemical properties data, please complete the following worksheet for data you provide and include it in the notice. Identify the property measured, the page of the notice on which the property appears, the value of the property, the units in which the property is measured (as necessary), and whether or not the property is claimed as confidential. If the attachments

are electronic, give the attachment number (found on page 12) at (b). The physical state of the neat substance should be provided. These measured properties should be for the neat (100% pure) chemical substance. Properties that are measured for mixtures or formulations should be so noted(% PMN substance in_). You are not required to submit this worksheet; however, EPA strongly recommends that you do so, as it will simplify review and ensure that confidential information is properly protected. You should submit this worksheet as a supplement to your submission of test data. This worksheet is not a substitute for submission of test data. Property Mark(X Page Value Measured o**Confi~** if number Estimate dential (a) provided (b) (c) Mark(X) (MorE) (d)



### Physical state of neat substance 

# D D . (s) 

# D(l) Dcg) D

Vapor pressure 

# D D @Temperature 

# oc Torr
Density/relative density 

# D glcm3 

# D

Solubility 

# D @ Temperature 

# oc 

# D

Solvent giL



### Solubility in water {jiJ 

### Temperature 

# oc 

# D giL 

# D

Melting teml'_erature 

# D oc 

# D

Boiling 

### I sublimation temperature(@ torr pressure 

# D oc 

# D

Spectra 

# D D

Dissociation constant 

# D D

Particle size distribution 

# D D

Octanol 

### I water partition coefficient 

# D D

Henry's Law constant 

# D D

Volatilization from water 

# D D

Volatilization from soil 

# D D

pH@ concentration 

# D D

Flammability 

# D D

Exl'_lodabili1y_ 

# D D

Adsorption 

### I coefficient 

# D D

Other

- Specify 

# D D

Other

- Specify 

# D D D Mark (X) this box if you attach a continuation sheet. Enter ihe attachment name and number.



### ATTACHMENT PAGE 13

## Page 000039 of 117 SAN



## Premanufacture Notice Electronic Continuation Sheet
Continued From: Part II, Section A1d, pg 8

Continuation Title: 

## null

Insert narrative continuation here 

### Contidential 

# D

,,



### Insert any picture here (PDF) 

### Contidential 

# D

D Check here to add another continuation sheet 

## Page 39of 40

## Page 000040 of 117 SAN


## Premanufacture Notice Electronic Continuation Sheet Continued From: Part Ill, pg 12

Continuation Title: 

## List of Attachments

Insert narrative continuation here Contidential 

# D

Insert any picture here (PDF) Contidential 

# D

D Check here to add another continuation sheet 

## Page 40 of 40

## Page 000041 of 117 SAN

CONSULTING LABORATORY



# Wastewater Treatability Report:·
Impact to Wastewater Treatment Process

Date of Report: August 15, 2008

Report Prepared For: Joseph Bretz South West NanoTechnologies 2501 Technology Place Norman, Oklahoma 73071

Report Prepared By: RespirTek, Inc. James Radich Vice President of Research

Laboratory Address: RespirTek, Inc. 12450 Shortcut Rd. Bldg.F Biloxi, MS 39532 Report Reviewed By: RespirTek, Inc. BJHook Technical Director

## Page 000042 of 117 SAN

CONSULTING LABORATORY



# Final Report

Date: 8/15/2008 Prepared for: SouthWest Nano Technologies- Joseph Bretz Project ID: CHA-21 01

Introduction The purpose of this project was to assess the impact of a low concentration discharge of a specific test compound provided by Southwest NanoTechnologies (SWeNT) relative to the on-site operations of Norman wastewater treatment facility (WWTF) in Norman, OK. SWeNT currently discharges to the WWTF, and prior to introducing new waste into the discharge stream, testing was conducted to evaluate the impacts to the WWTF. Analytical testing was conducted in conjunction with respiration monitoring as 

# a means for quantifying the potential impacts.

The Norman WWTF operates at an average influent rate of 10.5 MM GPO; however, a low-flow scenario was modeled to incorporate a worst-case basis for testing the additional waste component. The plant aeration basin operates between 5,400 and 6,400 mg/L total suspended solids (TSS). The discharge rate from SWeNT was provided by the project manager, and the test concentrations of the waste compound were agreed upon through discussions with the Norman pre-treatment coordinator prior to test initiation.



## Test Design and Setup Samples of the pre-treated waste material and standard discharge material from SWeNT arrived at the laboratory on 8 July 2008. Return activated sludge (RAS) and influent samples arrived on 9 July 2008. Two of the containers were outside of the recommended transport temperature of 2- 

# soc (11.6°C and 16°C). Appropriate actions were taken, and the temperatures were noted in the event of unexpected test results. Four aerobic 2-L reactors were set up following receipt of all samples according to the reactor charge matrix presented in Table 1.

Table 1. Reactor Material Charge



## Reactor ID Material Charge Rx 1 RAS +Influent Rx 2 RAS + Influent+ 0.001% Treatment Rx 3 RAS + Influent + 0.005% Treatment Rx 4 RAS + Influent + 0.023% Treatment

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## Page 000043 of 117 SAN

The influent was first decanted to remove additional solids present due to nonstandard operational conditions at the WWTP. RAS suspended solids concentration was 12,700 mg TSS/L upon arrival at the laboratory, and the appropriate volumes of RAS and influent were combined to model the WWTP's typical operating conditions in the aeration basin (-6,400 mg TSS/L). The test material supplied by SWeNT was prepared following guidance provided in an email from John Ferguson from Chasm on 8 July 2008. The goal was to model the discharge conditions from SWeNT and the treatment conditions at the WWTF
as closely as possible such that the only deliberate differences would be the input of the new waste compound at the specific test concentrations. The percent solids of the material supplied by SWeNT was taken into account in test execution.

After appropriate additions of materials were added to the preparatory flasks (4 liters), respective volumes of the homogenized material were added to the reactors and time 

# 0 sample containers. The reactors were then placed in a recirculating water bath at 20 °C and were allowed to equilibrate. Once ample time was given for temperature equilibration, the reactors were connected to the Challenge AER 200 Series Respirometer and aerobic respiration was monitored for 48 h. The reactors were stirred at -300 RPM to provide good saturation of oxygen to the aqueous phase and good mixing of the RAS and influent.
Samples were collected from each reactor at the completion of aerobic monitoring, and the following parameters were analyzed: ammonia nitrogen (NH 3 

## -N), biochemical oxygen demand (BOD), chemical oxygen demand (COD), nitrite, nitrate, and TSS. All analytical samples were filtered prior to analysis to quantify aqueous concentrations of analytes.



## Results and Discussion Results indicate that the compound tested has no significant adverse affects on the respiration of the biomass in the WWTP. RX 1 had a cumulative oxygen uptake value of 168.22 mg 0 2 /L; RX 2 uptake was 195.59 mg 02/L; RX 3 uptake
was 165.805 mg 02/L; and RX 4 uptake was 173.915 mg 02/L. Only RX 2 showed any significant difference in oxygen uptake in comparison to the baseline value with an increase of -28 mg 02/L. Respiration values for all other reactors
were within standard deviation limits of quality control criteria for the respirometric equipment and are considered insignificant. In terms of biomass respiration and implied metabolic activity, no negative impacts were observed. The lowest concentration of the compound, RX 2 

## @ 0.001%, may assist in metabolism of wastewater as indicated by the significant increase in biomass respiration.
Oxygen uptake rate values were relatively consistent amongst reactors with the exception of RX 3. The oxygen uptake rate for RX 3 shows some potential early inhibition (initial 12 hours) in relationship to the other reactors, but RX 3 had fully
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## Page 000044 of 117 SAN

recovered by 20 hours and was respiring at higher rates than other reactors for the duration of the testing. Other reactors showed relatively little difference in respiration rates over the incubation period. This further indicates a lack of any significant impacts to biomass respiration by the introduction of the new waste compound. Cumulative oxygen uptake and oxygen uptake rate are provided in Figures 1 and 2 in the Data Appendices.
Analytical results further indicate little, if any, significant impact to the wastewater treatment process. The waste compound exerts no observable differences in BOD values when time 0 BOD analytical results are compared amongst reactors. COD is slightly affected as indicated by a decrease in COD when compared amongst reactors. This phenomenon may be a result of some organic compounds within the wastewater adsorbing to the solid material tested. Nitrogen parameters (NH 3 

## -N, nitrate, nitrite) were unaffected by the addition of the test material to the reactors. All analytical results are provided in Table 1 in the Data Appendices.

After incubation and treatment of reactor bottle contents by the active consortia, all analytical values with the exception of nitrate increased. Several reasons exist to explain this phenomenon, but the results are in line with previous testing conducted at RespirTek from Norman's \MJVTF. Ultimately, BOD and COD increases are likely a result of the inability of the analytical tests to accurately quantify initial (high) values. BOD and COD tests are only as reliable as the ability of the test to break down organic compounds (BOD- microbial, CODstrong oxidant). Given historical data obtained from Norman's \MJVTF and the consistency of the analytical test results, the observed increases are not laboratory error.

Increases in NH 3 

## -N and nitrite indicate large molecules containing amino acids
are broken down as treatment progresses and are partially nitrified, albeit slowly and to a low extent. Observed increases in TSS are a positive finding, as this indicates continued growth of biomass during metabolism of wastewater organics. Increases in biomass in respective reactors were as follows: RX 1

- 160 mg/L; RX 2

- 200 mg/L; RX 3

- 150 mg/L; and RX 4

- 120 mg/L. The increases are in line with other observations relative to treatability, particularly the increase in biomass relative to baseline in RX 2.
Ending values for BOD show little difference between reactors. BOD results after incubation are as follows: RX 1

- 30 mg/L; RX 2

- 30 mg/L; RX 3

- 34 mg/L; and RX 4- 32 mg/L. The BOD results further indicate little, if any, impact to the treatment process by the addition of the test compound. Ending values for COD illustrate changes to the wastewater slightly more so than BOD. COD results after incubation are as follows: RX 1

- 115 mg/L; RX 2 - 112 mg/L; RX 3 - 119 mg/L; and RX 4- 139 mg/L. With. the exception of RX 4, differences in ending

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## Page 000045 of 117 SAN

COD values are not significant in relationship to test setup protocols and conduits for heterogeneous errors.
Overall, significant impacts to the treatment process at the Norman VWVfF are not observed in this study. Some slight positive (RX 2 oxygen uptake) and slight negative (RX 4 TSS, COD) impacts were observed but not with urgent significance. The treatability of the new waste compound is not clear from the results of the testing. The only indication of treatability of the material is in RX 2 in the form of increased respiration relative to baseline. Given the data obtained in the study, RespirTek concludes that no significant adverse impacts by the test compound were observed in the testing conducted. However, the actual fate of the test material is not clear but was not a specific goal of the study.

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## Page 000046 of 117 SAN



# Data Appendices

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## Page 

## 00004 7 of 

## 117 SAN

a e na1y11ca 

## T bl 1 A I 

## f I R esu 

## It S s ummary RX 1 

### I RX2 

### I RX3 Analytes (mg/L) Control 

### I 0.001".4 treatment 

### I 0.006% treatment

'· Ammonia aN 9.88 115 9.64 16 9.39 18.8 Carbonaceous BOD 12 30 11 30 11

. 34 Chemical Oxygen Demand 82 116 49 112 68 119 Nitrite 0.18 0.2 0.12 0.23 0.12 0.28 Nitrate · ND ND ND ND ND ND Total Suspended Solids 15,220 6,380 15,240 15,440 15,280 15,430 QA/QC Notes & Observations Holding Times: All samples analyzed within specified holding times Calibration: The instrument calibrations were within accepted criteria. Method Blanks: Method blanke were below MDL. Qualifiers: None

,-------------·-"-" 

### CumulativeOxygen Uptake

# ~ 150

# +-------------------------------~ ..

# ~----~--

.! J!



# ..**~** ::;, 

# J

100

# +-----------------~~--~~~--~,ti~L-----------------------

10 15 20 25 30 35 40 45 50
Tim a (h) 

## Figure 

## 1. Cumulative Oxygen Uptake

RespirTek, Inc. 12450 Shortcut Rd., Bldg. F, Biloxi, MS 39532 228.392.7977 I RX4 

### I 0.023% treatment

9.73 11.15 12 32 64 139 ND 0.12 ND ND 15,230 15,3150

-+-RX1- Control


### -a- RX2 • 0.001% Treatment

· ·-'•

... RX3- 0.005% Treatment

.......,_ RX 4 • 0.023% Treatment



## Page 6 

## of7

www. respirtek.com I I

### Page 000048 

## of 117 SAN

Oxygen Uptake Rate

-t- RX 1· Control

-11-RX2 • 0.001% Treatment

RX 3 • 0.005% Treatment

--;.+- RX 4- 0.023% Treatment

10 15 20 25 30 35 40 45 50 Time (h) 

## Figure 2. Oxygen Uptake Rate

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## 7 of7

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## Page 000049 of 117 SAN 

# ENSR AECOM

Report of**Short~** 

## Term Chronic Toxicity Testing using the Water Flea (Ceriodaphnia dubia)

Sponsor
Project Officer

Testing Facility

Study Director Report Author

Test Basis 

## Project ID: 08503-132-265-005 August2008



## Sponsor and Laboratory Information

SouthWest Nano Technologies 2501 Technology Place Norman, OK 73071 John Ferguson {405) 217-8388 ENSR Fort Collins Environmental Toxicology Laboratory 4303 West LaPorte Ave. Fort Collins, CO 80521 Fax: (970) 490-2963 State of Florida NELAP Laboratory ID: E87972 Rami B. Naddy, Ph.D. (970) 416-0916 ernail: 

## maddy@ensr.aecom.com Gina McNerney (970) 416-0916 email: 

## gmcnernev!alensr.aecom.com



## Test Information

Short-Term Chronic under Static-Renewal Conditions USEPA (2002), method 1002.0 Test Dates and Time August 15, 2008@ 1630 toAugust 22, 2008@ 1610 Test Length 7 days Species 

## Ceriodaphnia dubia
Test Material Processed material (grab); Material with carbon nanotube (CNT}r:>_aste Dilution Water Moderately Hard Reconstituted Water
Test Concentrations 0 (centro?, 0.5, 1, 5, 32% material, and 5% material+ CNT (0.023%)

. .

1 

### Target concentration was 0.5% material but do to an error, actual concentration was 5%; sufficient volume was prepared on day 0 and used throughout the study. The sample was stored at 4°C in the dark with no head space when not in use. CNT paste was added as received.

ENSR 

### Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page 1

## ENSR Page 000050 of 117 SAN**08503-132-265~005**



## Material Collection and Receipt

Note: See Appendix A for chain of custody records



## Material Characterization

Initial Dilution/Control Water Characterization

b 

### Total residual chlorine

c 

### Measured in source water



## Test Conditions

Type Static-Renewal Short-term Chronic Test Endpoints Survival and Reproduction Test Chambers 30-ml plastic cups Test Solution Volume 15 ml Replicates per Treatment 10 Organisms per Replicate 

## 1 Test Temperature 25 

## ± 1°C Lighting Fluorescent, 16 hours light:8 hours dark Chamber Placement Random block, according to computer-generated chart Aeration None Test Solution Renewal Daily

ENSR 

### Fori Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page2

## ENSR Page 

## 000051 of 

## 117 SAN 08503-132-265-005



## Test Organism 

## Species Ceriodaphnia dubia 

## Age <24 hours (all within 8 hours) Source ENSR in house culture, batch 081408 Acclimation Test temperature Feeding 0.2 ml YTC/Aigae mix per test chamber, daily during the test Reference Toxicant Testing Initiated August 19, 2008 using sodium chloride (NaCI)

.

TEST RESULTS


## Biological Data

0 (Control) 

## 100 100 100 90 90 90 90 

# N/A 23.1 

# N/A N/A

0.5 100 100 100 100 100 100 100 

# N/A 22.3 No No



## 1 100 100 90b 90 90 90 90 N/A 23.8 

## No 

## No


## 5 100 100 100 90 90 90 90 N/A 10.6 No Yes

32 0 0 

## 0 0 0 

## 0 0 

## N/A 

## 0.0 Yes
5+CNT 

## 100 100 100 100 100 80 80 

# N/A 7.4 No Yes

Percent Minimum Significant Difference (Reproduction)c 

## 50.1 

## Elevated

Mean young per original female. any or higher were**JJrv~•uc.;~::,u,** 

### mean young per female and statistical analysis of reproduction. bOne test organism was not found and was considered a mortality

c 

### PMSD value was determined using Dunnett's Test (a 

### = 0.05) (Toxstat Version 3.5; WEST, Inc. and Gulley 1996). Note: See Appendix B for copies of laboratory data sheets



### ENSR Fort Colllns Environmental Toxicology Laboratory NELAC Accredited Page 3

## ENSR Page 000052 of 117 SAN



## Data Analysis

Survival Significant Reduction Relative to the Control
Normality 8


## Homogeneity of Variance 8 

## Reproduction Significant Reduction Relative to the Control 8



## Threshold Inhibition Concentration (IC 25 )b



### bUsing Norberg-King (1993)



## Test Endpoints

Survival

. 

# NOEC LOEC NOEC

ChV 

# LOEC Reproduction

NOEC 

# = No 

### Observed Effect Concentration LOEC 

## = Lowest Observed Effect Concentration ChV 

# = Chronic Value IC2s 

# = 25% 

### Inhibition Concentration

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

## 08503-132-265-005

Inspection Shapiro-Wilk's Test

Bartlett's Test

Linear Interpolation

## :,;i!(ff{o:\M~~~~~·~·il:T:.::l:lW::·:::: 5 

## 32 1 5 

## 2.2 2.84

Page4

## ENSR 

## Page 000053 of 117 SAN 

## 08503-132-265-005

Physical and Chemical Data



## 0 (Control) 

## 8.0 8.3 6.3 7.1 

## 273 298 24 25
32 

## 7.7 7.8 6.2 6.7 920 996 25 

## 26 24b 26b All Treatments 

## 7.7 8.3 ;::5.9 8 

## NA 

## 24.0c 26.0c

Performance NA Acceptable NA Acceptable

saturation
b 

### Temperature In test solutions

c 

### Continuous wet temperature in the environmental chamber



## Reference Toxicant Test Results for C. dubia



## Procedural Deviations 

## Temperature, pH, and dissolved oxygen were not measured in old solution from any test treatment on day 4. This procedural deviation likely had no impact on the outcome of this study. To the best of the Study Director's knowledge, no further deviations from the test procedure occurred during the study.

References 

## Norberg-King, T. 1993. A linear interpolation method for sublethal toxicity: the inhibition concentration (ICp) approach, version 2.0. National Effluent Toxicity Assessment Center, Technical Report 03-93.
US EPA. 2002. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. Fourth Edition. EPA-821-R-02-013. WEST, Inc. and D.O. Gulley. 1996. Toxstat Version 3.5. Western EcoSystems Technology, Inc., Cheyenne, WY.

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page 5

## ENSR Page 000054 of 117 SAN



## Statement of Quality Assurance

The test data were reviewed by the Quality Assurance Unit to assure that the study was performed in accordance with the protocol and standard operating procedures, and that the resulting data and report meet the requirements of the NELAC standards. This report is an accurate reflection of the raw data.

# ~~ Quality ASSUanCeunit

# ~ t:t., 

# woi
Date

Data Analyst

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page6

## ENSR Page 000055 of 117 SAN

APPENDIX A


## Chain of Custody Records

ENSR Fort Collins Environmental Toxicology Laboratory NELAC AccreditedIENSRIAECOMCHAINOFCUSTODYRECORDPage_J_of'2.IClient/ProjectName:sLProjectLocation:pa--rlJContainerT:,ml!;l'lt!seJVationAnalysisRequestedP-PlasticA-AmberGlass2-H2S04,4°G-Cle<orGlass3-HN03,4"ProjectNumber:'g("-o3--l?z-ReidLogbookNo.:V-VOAVial4-NaOH,4"0-0ther-:I''*t=o(i01t<-lE-Encore5-NaOHIZnAc,4"6-Na2S203,4•Sampler{PrintName)/{Affiliation):ChainofCustodyTapeNos.:7-4•"So\......-C:\......'1:.....,DW-DrinkingWaterS-SoilWW-WastewaterSL-Siudge/!2SendResults/Reportto:TAT:GW-Groundwaterso-Sedimentct.M......SW-SurfaceWaterSO-Solid*--ST-StonnWaterA-/>Jr....-tr.e."-l).eo..W-WaterL-liquidP-Pr'oductFie(SamplecGSample0RFieldLabDateTimeMAcontainerMatrixPreserv.Filtered1.0.Remarks....,Lp8(Size/Mat'l).I@@IX}(,L;1,nttL+O'l'ttl(J)IV--f,.lg00())lo....->.-'----.11(1)Relinquishedby:(PrintName)I(Affiliation)Date:....t"'1-Receivedby:(PrintName)I(AIIilialionlDate:7/Jc-tBAnalyticalLaboratory{Destination):)"J¢\.._.J..0\'\-\.c:c:..€-(,"c.(\HH<(')0ENSRToxicologyLabSiqnature::.....:\...--Time:..•1..4lD_:.J.c_.Time:0150.4303W.LaporteAvenueoal(?'..4":Receivedby:(PrintName)I(AffiliationlDate:FortCollins,CO80521'6:5e.•_...(970)416-0916»'co.}!_,Signature:Time:(970}490-2963(FAX)Signatur¢-Date:Receivedby:(PrintName)I(Affiliation)Date:SampleShippedVia:ITempblankSi!lnature:Time:Sianature:lime:FedExCourierOtherYes(1\jo")I-.-...1'\.tmA-twGmt1nn7Me..,J,LfleUJOoSerial482·39·.=-:·:_·:···::;....·.··No·=·altt·--,'::··.{1'_..•;'.Respirtek,Inc.Laboratory12450ShortcutRdSuiteFBiloxi,MS39532Phone:228-392-7977Fax:228-392-7838Email:bjhook@respirtek.comCHAIN-OF-CUSTODYRECORD(DOCPD-003)Page__ofP.O.No.tTProjectNameANALYSESAddressResultsTo:,Samplers:1.1\fu.(Signatures)2.----''\::_____-_"1________3.-------------------SampleI.D.DateTimeCGColiNo.ofrByCona1,2,3tainersREMARKSb0\UV\1,?,1...,!Y"IJ·?rfi!\QI4·f!JfPfJhlt1fiAA{,ltfJ\Ait.\1)\uJ1At';VInA-tIJ!o1....-:::..00-...................I-- !!lReRelinquishedby:DateJ};)Jt:{?zp_T1ReltJ.quisliedby:-IDateTimeReceivedby:Relinquishedby:Date.TimeReceivedt''or'CJCLaboratoryby:Transportedby:DATEOFANALYSIS:ANALYSISTOBECOMPLETED:REMARKS:ALLSAMPLESICEDINTHE_CALLOUT__NORMALTURNAROUNDPERIODFIELDANDDURINGTRANSPORTTOTHERECEIVED:LAB.YESNO__PRIORITY(NOCALLOUt)__CALLWITHRESULTS- -COMPLETED:UPONCOMPLETIONOriginalCopy-LABCopy-FILE

## ENSR Page 000058 of 11 7 SAN

APPENDIX B



## Test Data

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited Page B-1

## Page 000059 of 117 SAN Page 1 of 

# __11_ FCETL QA Form No. 

### 051 Revision 

### 4 Effective 

### 1/96



### TOXICITY DATA PACKAGE COVER SHEET

# ~**~I¥J}otb ~:**  

# !:nt0etlo't·Lor
Test Type: Chronic Project Number: 

### __,o...,.a

...**s..._03,._-....l.1~32=---=26~5~-** 

### ....**oo~s~-**


### Test Substance: Effluent Species: Ceriodaphnia dubia



### Concurrent Control Water Type: NA Organism

# Lot~mber: 0811./0&

Age: 

### £. 

# 2.1./Jtij< 24 hr) Supplier: FCETL**__.;......;;...;.;;;~----** 

### Dilution Water Type: Mod Hard

Date and Time Test Began: 

# /kjo<l @

# /~3 o
j 

### Protocol Number: USEPA 

### 2002 

## + Reg. VI

· Background Information pH control?: Yes

# ~ If yes, give 

### % C0 2 : 

# 'iliA

Env.

# Chm~. '*CD_T_e-st_C_h-mbrs: 30-ml cups Type of Test:**Static~Renewal** 

### Test Temperature: 

### 25 ± 

### 1 

# oc

Test Solution Vol.: 

### 15 ml Number of Replicates per Treatment: 

### 10 Length ofTest: 

### 3 broods Number of Organisms per Replicate:

_____**1~---** 

### Type of Food and Quantity per Chamber: 

### 0.2 ml YTC/ALG Feeding Frequency: 

### 1 x Daily

Test Substance Characterization Parameters and Frequency:
Hardness: Sx Receipt Alkalinity: Sx Receipt NH 3 : 

### Sx. Receipt TRC: Sx Receipt pH: Daily Conductivity: Daily and Test Termination* 

# (i) MH, 

### 0.5, 1, 5, 32,

## and~ + CNT effluent

. Test Concentrations (Volume:Volume):

Reference Toxicant Data: Test Dates:

Hist. 

### 95% Control Limits: to Method for Determining Ref. Tox. Value:

Special Procedures and Considerations:

•conductivity measured in dilution**w~ter** 

### and 

### 100% effluent at test termination D.O. maintained C!: 

### 4.0 mg/L

Study Director Initials:



# . '(lrZf--) Date:

# v/lr;/o~

. (9Cfuvl**011~105** 

## € 

# lcM:-e..

### Project Number:



## MH 0
0.5% 

### 7



### 1
5% 72.5 

## Page 000060 of 117 SAN

TEST SUBSTANCE USAGE LOG**08503-132~265-005**



### 1450 1450
1443 1450
436 1450
1377.5 1450 Page2ofj]_ FCETL QA Form No. 041 

### Revision 4 Effective 1/96

# I

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# MH 

# I 1
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## 3
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## 6
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# I 1
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## 4
5


## 6
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1% 

# I 1
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## 4 5
6
7 8 Total Day: Date: Time: Initials: Page 

## 000061 of 

## 117 SAN

CERJODAPHNIA DUB/A CHRONIC BIOLOGICAL DATA 

## Page3of 

# 13 FCETL 

### QMorm No. 057 Revision 1 Effective 2/93



## Number of Neonates Produced and Survival of Original Organisms
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# II Project Number: 08503-132-265-005 

## Page 000062 of 117 SAN

CERIODAPHNIA DUB/A CHRONIC BIOLOGICAL DATA Page_ 

### 4_of 

# _fl FCETL QA Form No. 057 Revision 

## 1 Effective 2/93



### Number of Neonates Produced and Survival of Original Organisms
Cone. Day A 

### B 

# c D 

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## Page 000063 of 117 SAN

CHRONIC CHEMICAL DATA (INITIAL)


## Project Number: 08503-132-265-005

Test Species: 

## Ceriodaphnla dubia

% Day Day Day Day Day Day 

## 1 2 3 4 5 6 Cone.: 

# IEJ

IPH

# ~'I D.O. (mg/L} 

# tR·1> Temp. 

# COCl -z..5 Cond. (IJS/cm) 

## 1.-&\ (o Hard. (mglL) 

# g{a 

# qo Alk. _{mg/L) · 

# (0_:.\ bl TRC (mg/L) 

# 'ith<tl08 <0,{)2 

# o.ot.. NHa (mg/L) 

# <\,o*
Cone.: 

# II 0.5%



## D.O. (mg/L) Temp. (°C)**~.** 

# ¥**-~·** 

### Cond. {J.l.S/cml 

# 31 (**~o5** 

# -oo lS'
Hard. (mg/L) 

## Alk. (mg/L) TRC (mg/L) 

## NH 3 

### (mg/L)


## Cone.: 111.0% 

# 3

.. 

# I
D.O. (m__g/L}

## ~·l 

# ( 0 

# /1 Temp. 

# eCl**~** 

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# 2ll
Cone.: 

# II 5.0% 

# I

Initials:

## ~ 

# .l:,**~** 

# At<- 

# JrK r2>Ff' Ak' Day 

## 7 

## Page _5_ of 

# J1 FCETL QA Form No. 058 Revision 3 Effective 3/98

# ~ 

# 1lt 

# }o'h QA-:,**Mb'1 lo'1;lo~** 

Meter# Remarks

All 

### Cone. 

# '"**~** 

## 037



# ,;;
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# ·r\\YL

fU) 

# M·-1

Note: Hardness, alkalinity, TRC, and NH3 data appearing on thls page have been transcribed from the wet chemistry log FCETL QA Form No. 084.

.

## ~~O.Su.rtJ 

# iV! SVU.Vc.t

## ~W *Dillutlon/control water and effluent were brought to 

### 25•c prior to making the dlllution series. The temperature of resulting effluent dilution is assumed to also be 25"C.**~** 

# Jrj( 

# % [

{9lo '/,**~**

## Page 000064 of 117 SAN

CHRONIC CHEMICAL DATA (INITIAL)


## Project Number: 08503-132-265-005


## Test Species: Cerlodaphnla dubia

%



## cone.: 

# 1132% 

# I**~** [pH 

# 7. C6 

# 1\ t$
D.O. (mglL) 

# (o.; U· 

# b

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## 2,.0**~U\\** 

## Cone.:**~CNT**



## Cone.: 

# II [pH D.O. 

## (mg/L}

Cond. (IJS/cm) Cone.: U [pH D.O. (mg/L)



## Cond: (IJS!cm) Cone.: 

## 11100% 

# I

IPH 

### @ 

# ·1.0
D.O. (mg/L}

- 

## Temp._CC_l 

# 7.15' Cond. (J.JS/cm} G) 

# \3**\~** Hard.lm_g/L) {:)9 

## {Q 

# 7 2 Alk. (mg/L) 

## @ 

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# ® ..(0,02 NH 3 

### (mg/L) 

## @.) 

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Initials: 

## }f 

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# --:li,.**-~** 

# !if lS# Prlt Page 

# _a_ of 

# \o FCETL QA Form No. 

## 058 Revision 

## 3 Effective 3/98

# ~ tt/8/D'a tl)f+ l

# ~'l-lo<Hoi

Day Meter#



## 7 All 

## Cone. Remarks

Note: Hardness, alkalinity, TRC,**and~H3** 

### data**appearin~n** 

### this Gage have been**tran~Cflbtd** 

### from the wet chemh\try log FCETL QA Form No. 084. 

# £)**W~** -,

# ~e~**0~** 1o

# tf..t.tA.t~ 1

# ~ 

# \o'tJ M

# ~re~wt'olftU')piJ-. •oillu1ion/control water and effluent were brought to 25"C

### ·rio~ to a'K.ing the dillution series. The leTperature of resulting effluent dilution is assumed to also be 25"C. 

# @ tt.J'V' 

# q Q 

# 10 

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# Gr)Wl 

# 'ljt?J o& 6

# I %



## Cone.: pH D.O. (mg/L) Temp ( 0 

### C} 

## Cone.:
IPH D.O. (mg/L) Temp (°C) Cone.: pH D.O. (mg/L) Temp (°C} Cone.:
IPH D.O. (mg/L) Temp (°C) Cone.: pH D.O. {mg/L) Temp (°C) Cone.:
IPH D.O. (mg/L} Temp (°C) Cone.:
IPH D.O. (mg/L) Temp (°C)


# \ Page 000065 of 117 SAN

· Cerioda hnla dubia 

# I Day Day Day Day Day Day Day Day 1 2 

## 3 4 

## 5 6 

## 7 

## 8 

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# a o&**a,fj-~M0<11>4 ~** 



## Meter# Remarks

All Cone. 

## * conductivity

"' conductivity



## * conductivity



# --)6NOIG ; PIY'H\,\ 

# c.hunlst:

### Page 000066 of 117 SAN



## DAILY TOXICITY TEST LOG Project Number: 08503-132-265-005 

### Test Species: Cerlodaphnla dubia



### General Neonates obtained from

## ~!'~!~~**!f~~(f-.tt.:~~erio** monoculture board Comments

## (~ra-\v marked cups)

Test Day 0 Test Solution Mixed at:**~~~S'"** 

### :est Organisms Added at: 

# 1**~:;o**



# .. Test Day 1

# CT~~ 2(c

.. O**Ra~=** 

## C(4l**tt~c** 

# ;;,r;;.G 

# •c. 

# oc
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## '2->.ti}

. .- 2(,,. 

# 0 °U

Test Day 2

### CT~°C

## Range~¥ 

# tf d2G':&- 

# oc

. I**~** 

## 210--0 <(.... 

# z.c.,. 0

........ 

## 2.(;,. 0



### Test Day 3

## CT~°C

# Rang~· 

# q., tf -;;x;

. 

# .iF @-z-5:.2... 1...- 

### 'l.L{.(o- 

# -z..rc:.. 0 °G

Test Day 4

## CT=o{~./p 

# oc. Range= 

# J?4.**~** 

# e)-j. (c 

# ac

{f) Test Day 

## 5 CT=2.lhb 

# oc Range= 

# ?.'·M_f- '2-Lf.(i, 

# oc

. 2

Test Day 6 CT= f:}L\,?_°C Range= 

# d) 

# Lf( 0 -d 

# y,**~c**



### Test Day 

## 7 CT= 

# oc Range= 

# oc**@~,.~**

# ~~**):;\-·~** 

# s .e t..Y.

.. 

# O '2-4**~** 

# ()- 

# 24 .1,;

Test Day 8 CT= 

# oc Range= 

# oc**~D.f-:J** 'tf(UJ/Of}' 

# E (O..W\

## "'11.~1()~ 

# w I

Page 

# _a_ of 

# \& FCETL QA Form No. 055 

### Revision 2 Effective 1/94 _p / 

# dlt; fJ?t(f}jlR 

# /0'6

Feeding Initials/Date
0.2 ml YTC/ALG 

### Daily 

### Fed@

# !~2-S" 

# At>**'tJif/6~**

Fed @ro/fo

# ~ 

# et 1c. 

# bJe

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# s**w~t}ct** Fed@

'1\ 

## 0YH!

...

## r~r\\**~~'2.~/C6**RandomBoardID:HProjectNumber:8503-132-265-005ReplicateABcDETreatment3 1525#52243246324546413151663161=Control(ModHard)2=0.53=14=55=326=5+CNT0k6treatmentsX 10reps-FGHI435322461 13136 6254246515Page.1_of.JlClJ413265"'Uru.aCD000.......s.'->.I,.......!-.J!en')>z

### Title: File: 

## Page 000068 of 117 SAN

8503-132-265-005 CC 8/15-22/08 repro/original 265005R1.dat Transform:

# CJI!vutf~ tp(/:il
Page_ffi_ot.ll_

Summary Statistics on Data NO TRANSFORMATION 

## d 

## afr"-. 

# /tYW r; 

# /z:J lo**~** TABLE 

### 1 of 2

GRP IDENTIFICATION

1 

### 2 

### 3 4 5 

### 0 0.5 

### 1 5 

# 5' j)A''cNT N



### 10 10 10 10 10 MIN

0.0000 1. 

### 0000 0.0000 0.0000 1.0000 MAX



### 43.0000 33.0000 37.0000 29.0000 18.0000 MEAN

23.1000 22.3000 23.8000 10.6000

7.4000**---------------------~-----------------------------------------------------** 

### Summary Statistics on Data TABLE 2 of 2

GRP IDENTIFICATION VARIANCE 

## so SEM 

# c.v. %

1 0 189.8778 13.7796 4.3575 59.6520 2 0.5 210.6778 14.5147 4.5900 65.0885 3 1 113.2889 10.6437 3.3658 44.7215 4 

### 5 111.8222 10.5746 3.3440 99.7604 5 

# 5**~CNT** 48.7111 6.9793 2.2071 94.3153 

# ---------------11>----------------------------------------------------------

Title: 8503-132-265-005 CC 8/15-22/08 repro/original File: 265005Rl.dat Transform:



## D 

# = 6069.4000 

# w = 0.9438 Shapiro

- Wilk's Test for Normality

Critical W 

# = 0.9300 (alpha 

# = 0.01 , N 

# = 50) W 0.9470 (alpha 

# = 0.05 , N 

# = 50)

Data PASS normality test (alpha 

# = 0.01). Continue analysis.

Title: 8503-132-265-005 CC 8/15-22/08 repro/original File: 265005Rl.dat Transform:

Bartlett's Test for Homogeneity of Variance NO TRANSFORMATION

NO TRANSFORMATION

Calculated Bl statistic= 5.1038 (p-value = 0.2768) Data PASS Bl homogeneity test at 0.01 level. Continue analysis.

Critical B 13.2767 (alpha 9.4877 (alpha 0.01, df 0.05, df 

## 4) 4)

## Page 000069 of 117 SAN

Title: 8503-132-265-005 CC 8/15-22/08 repro/original File: 265005Rl.dat Transform: NO TRANSFORMATION

SOURCE

Between Within (Error)

Total

Critical F OF

4 

### 45

49 ANOVA Table



# ss

2436.9200 6069.4000

8506.3200

3.7674 (alpha= 0.01, df = 

### 4,45) 2.5787 (alpha= 0.05, df 

# = 4,45) MS

609.2300 134.8756 F

4 

### '5170

(p-value = 0.0037)

Since F 

### > Critical F REJECT Ho: All equal (alpha 0.05)

Title: 8503-132-265-005 CC B/15-22/08 repro/original File: 265005Rl.dat Transform: NO TRANSFORMATION

Dunnett's Test TABLE 1 OF 2 Ho:Control<Treatment

TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T 

### STAT 0.05



# -------------------- 

# ----------- ------------------ ------ 1 0 23.1000 23.1000 2 0.5 22.3000 22.3000 0.1540 3 1 23.8000 23.8000 -0.1348 4 5 10.6000 10.6000 2.4067 

## * 5 

# Q) S' 

# 0 CNT 7. 4000 7.4000 3.0229 

# *



# ---------------------------------------------------------------------------- Dunnett critical value 

# = 2.2300 (1 Tailed, alpha= 0.05, df [used] = 

### 4,40) (Actual df 

# = 4,45)

Dunnett's Test TABLE 2 OF 2 Ho:Control<Treatment

GROUP IDENTIFICATION NUM OF REPS

1 0 10 MIN SIG DIFF 

## % OF DIFFERENCE (IN ORIG. UNITS) CONTROL FROM CONTROL

2 0.5 10 11.5821 50.1 0.8000 3 1 10 11.5821 50.1 -0.7000 4 5 10 11.5821 50.1 12.5000

# --~----------~-~-~-=~~----~~-----------==~=~:=-------=~~~------==~~~~~--

## Page 000070 of 117 SAN

08503-132-265-005

Cone. ID 1 

### 2 

### 3 4 5



### Cone. Tested 0 

### 0.5 1 

### 5 32
Response 

### 1 21 

## 2 12 19 0 Response 2 39 33 37 27 0 Response 3 0 30 27 29 0 Response 4 20 31 0 5 0 Response 5 24 

### 1 25 4 0 Response 6 

### 27 32 22 0 0 Response 7 25 29 28 9 0 Response 8 30 1 28 3 0 Response 9 2 32 26 8 0 Response 10 43 32 33 2 0



# -------------------------------------------------------------- *** Inhibition Concentration Percentage Estimate 

# *** Toxicant/Effluent: effluent Test Start Date: 8/15/08 Test Ending Date: 8/22/08 Test Species: Ceriodaphnia Test Duration: 7 days DATA FILE: 265005R.icp OUTPUT FILE: 265005R.i25

Cone. Number Concentration Response Std. Pooled

ID 

### Replicates % 

### Means Dev. Response Means



# ----------------------------------------------------------------------- 1 

### 10 0.000 23.100 13.780 23.100 2 10 0.500 22.300 14.515 23.050 3 10 1.000 23.800 10.644 23.050 4 10 5.000 10.600 10.575 10.600 5 10 32.000 0.000 0.000 0.000

The Linear Interpolation Estimate: 2.8394 Entered P Value: 25

Number of Resamplings: 80 The Bootstrap Estimates Mean: Original Confidence Limits: Resampling time in Seconds: 2.5011 Standard Deviation: 1.2735 Lower: 0.4866 Upper: 5.3125 0.00 Random_Seed: -366960776

## Page 000071 of 117 SAN

# Page:~ 

# If 

# 1]. FCETL QA Form No. 15 Effective: 5/90

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# r/-o 8 SUBJECT: DAILY LOG

ALL ENTRIES MUST BE INITIALLED WITH DATE AND TIME:



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## Page 000072 of 117 SAN 

# ENSR AECOM

Report of Short· Term Chronic Toxicity Testing using the Fathead Minnow (Pimephales promelas)

Sponsor
Project Officer

Testing Facility

Study Director Report Author

Test Basis 

## Project ID: 08503-132-265-006 August 2008

Sponsor and Laboratory Information

SouthWest Nano Technologies 2505 Technology Plaqe Norman, Oklahoma 73071 John Ferguson (415) 217-8388 ENSR Fort Collins Environmental Toxicology Laboratory 4303 West LaPorte Ave. Fort Collins, C0.80521 Fax: (970) 490-2963 State of Florida NELAP Laboratory ID: E87972 Rami B. Naddy, Ph.D. (970) 416-0916 email: maddy@ensr.aecom.com 

## Rami Naddy {970) 416-0916



## Test Information

Short-Term Chronic under Static-Renewal Conditions USEPA (2002), method 1000.0 Test Dates and Time August 15, 2008 

## @ 1630 to August 22, 2008 

## @ 1550 .. 

## Test Length 7 days Species 

## Pimephales promelas
Test Material Processed Material (grab}; Material with carbon nanotube {CNT) paste Dilution Water Moderately Hard Reconstituted Water
Test Concentrations 0 (control}, 0.5, 1, 5, 32% Material and 5% Material 

# + CNT {0.023%)'

1 

## Target concentration was 0.5% material but do to an error, actual concentration was 5%; sufficient volume was prepared on day 0 and used throughout the study. The sample was stored at 4°C in the dark with no head space when not in use. CNT paste was added as received.

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page 1

## ENSR Page 000073 of 117 SAN 

## 08503-132-265-006

Material Collection and Receipt

Material Characterization

Initial Dilution/Control Water Characterization

b 

### Total residual chlorine

c 

### Measured In source water



## Test Conditions

Type Static-Renewal Short-term Chronic Test Endpoints Survival and Growth {Dry Weight Per Original Fish) Test Chambers 500-ml plastic cups Test Solution Volume 250ml Replicates per Treatment 5 Organisms per Replicate 

## 8 Test Temperature 25 

# ± 1°C Lighting Fluorescent, 16 hours light:S.hours dark Chamber Placement Random according to computer-generated chart
Aeration Initiated on day 3 because of low DO in 5% 

## + CNT treatment; ta[get of 100 bubbles per minute Test Solution Renewal Daily

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page2

## ENSR Page 

## 000074 of 

## 117 SAN 08503-132-265-006



## Test Organism 

## Species 

## Pimephales promelas Age <24 hours Source ENSR in house culture, batch 081508 Acclimation Test temperature Feeding 0.1 ml brine shrimp nauplii per test chamber 3x/day durina the test Reference Toxicant Testing Initiated August 8, 2008 using sodium chloride (NaCI)

TEST RESULTS 

## Biological Data

0 (Control} 

## 100 100 100 100 100 100b 0.586 

## N/A N/A



## 0.5 100 100 

## 100 100 97.1c 97.1 97.1 0.571 

## No No



## 1 

## 100 97.5 97.5 97.5 97.5 97.5 95 0.501 

## No No



## 5 

## 100 100 

## 100 100 97.1c,d 97.1 97.1 0.504 

## No No


## 32 0 0.000 

## Yes

5+CNT 

## 100 100 

## 100 

## 100 100 100 100 

## 0.546 No No



## Percent Minimum Significant Difference (Growth) 6 

## 18.0 

## Acceptable
based on the original number of organisms per replicate.
b 

### Test organisms in replicate B were excluded from analysis because aeration was too vigorous in this treatment, which lead to Increased mortality; 3 organisms in replicate D were also excluded from analysis due to a similar problem. This did not appear to be a problem in other replicates or treatments. cOne organism was accidentally killed during the test due to a technician error and excluded from statistical analysis. dOne test organism was not found and was considered a mortality.

e 

### PMSD value was determined using Bonferroni t-Test (a= 0.05) (Toxstat Version 3.5; WEST, Inc. and Gulley 1996). Note: See Appendix B for copies of laboratory data sheets.

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page3**----------------------------~~----**

## ENSR

Survival

Growth (per original fish) Page 000075 

## of 117 SAN



## Data Analysis

Normality Homogeneity of Variance niflcant Reduction Relative to the Control Normality a 

## Homogeneity of Variance a



## Threshold Inhibition Concentration (IC 25 )b 

### (WEST, Inc. and 

### Gulley 1996) bUsing Norberg-King (1993)



## Survival

Growth (per original fish)

NOEC 

## = No Observed Effect Concentration LOEC = 

### Lowest Observed Effect Concentration ChV 

# = Chronic Value IC25 

## = 25% 

### Inhibition Concentration 

## Test Endpoints 08503-132-265-006

N/A
N/A 

## Inspection Shapiro-Wilk's Test (a=0.01)
Bartlett's Test (a=0.01)

Linear Interpolation

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page4

## ENSR 

## Page 000076 of 117 SAN 08503-132-265-006



## Physical and Chemical Data

0 (Control) 

## 7.8 8.2 5.8 7.1 273 301 24 

## 26 32 7.8 7.9 6.4 6.7 641 

## 948 25 

## 26 24b 26b All Treatments 

## 7.6 8.3 

## ::::3.7a NA 

## 24.0° 26.0°

Performance NA 

## Acceptable NA 

## Acceptable

DO of
b 

### Temperature in test solutions

c 

### Continuous wet temperature in the environmental chamber



## Reference Toxicant Test Results for P. promelas

Procedural Deviations

The bubbling rate during aeration to increase the dissolved oxygen was observed to be too high
on days 6 and 7 because of an equipment malfunction. This was the likely cause of the observed mortalities of the organisms in the control treatment. Because the observed mortalities in the control treatments were believed to be caused by excessive air flow, these organisms were excluded from statistical analyses. To the best of the Study Director's ·knowledge, no further deviations from standard test procedures occurred during the study.



## References
Norberg-King, T. 1993. A linear interpolation method for sublethal toxicity: the inhibition concentration (ICp) approach, version 2.0. National Effluent Toxicity Assessment Center, Technical Report 03-93.

US EPA. 2002. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. Fourth Edition. EPA-821-R-02-013.
WEST, Inc. and D.O. Gulley. 1996. Toxstat Version 3.5. Western EcoSystems Technology, Inc., Cheyenne, WY.

ENSR 

## Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page 5

## ENSR Page 000077 

## of 117 SAN 

## 08503-132-265-006



## Statement of Quality Assurance



## The test data were reviewed by the Quality Assurance Unit to assure that the study was performed in accordance with the protocol and standard operating procedures, .and that the resulting data and report meet the requirements of the NELAC standards. This report is an accurate reflection of the raw data.

Quality Assurance Unit Date

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page6

## ENSR Page 000078 

## of 117 SAN 08503-132-265-006



## APPENDIX A

Chain of Custody Records

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

### Page A-1!ENSRIAECOMCHAINOFCUSTODYRECORDPage.J_of7-lClient/ProjectName:sl.Je..ProjectLocation:pcf--ruPreservatigoAnalysisRequestedP-Piastic1-HCI,4"A-AmberGlass2-H2S04,4•G -ClearGlass3-HN03,4•ProjectNumber:?z-2{?FieldLogbookNo.:V-VOAV.al4-Na0H.4°0-0iher.E-Encore5-NaOHIZnAc,4°6-Na2S2Q3,4•Sampler(PrintName)I(Affilialion):ChainofCUstodyTapeNos.:7-4°""J'.s\..,...-6,.,,.....\..,J-t7-:'!D"ff-'=f(rr.!r&d-)MatrixCod!!:!iOW-OrinJdngWaterS-Scil...-?;it/1JI-WW-WastewaterSL-SfudgeSendResu!ts/Reportto:TAT:GW-GrotJndwaterSO-Sediment...-u.e.......&N-SurfaceWaterso-SoridST-StormWaterA-Air....ea..'""W-WarerL-LiquidP-ProductFie(SampleNoJidentificati:cGSample80RReidLabDate.limeMAContainerMatrixPreseN.Rlteredr.o.Remarks)._p..B(Size/Mat'l)Je#tl.)e/)L)<,.,.,flwIQ00<D0-->.-....!lrn)>zRelinquishedby:(PrintName)I(Afliliation)Date:Receivedby:(PrintName)I(Afliliation)Date:if1AnalyticalLaboratory(Destination):)-:Jd\..._.._on€-C,"c.(\>-4\I!Signature:\...---Time:"(IW.,.Signature:liJ•....Time:OlSOENSRToxicologyLab.4303W.LaporteAvenue.Receivedby:(PrintName)I(Affiliation)Date:FortCollins,CO80521es$e.•'i!£"'7'.,?....·(970)41fKI916Signatuw,U>.Signature:·Time:(970)490-2963(FAX)Date:Receivedby:(PrintName)I(Afliiation)Date:ISampleShippedVia:lTempblankiSignature:Time:Sil:mature:Time:FedExCourierOtherYesR.Coli'ts1'007.docSerial482-39·Respirtek,Inc.12450ShortcutRdSuiteFBiloxi,MS39532Phone:228--392·7977Fax:7838Email:bjhook@respirtek.com:':.:_:·....:..·.··....iiCHAIN-OF-CUSTODYRECORDQlOCPD-003)Page__ofP.O.No.Samplers:1.(Signatures)2.3.SampleI.D.IDateITimecIGconINo.oft.J\LIY'llb[...lah!AJAI16Rel{rlquisb.edby;-,DateITimeREMARKS:ALLSAMPLESICEDINTHEFIELDANDDURINGTRANSPORTTOTHELAB.YESNO'copy-LABCopy-FILErByCona1,2,3tainersbReceivedby:Receivedby:Transportedby:ANALYSESRelinquishedby:Relinquishedby:DATEOFANALYSIS:RECEIVED:COMPLETED:DateITime'·1/li)DateITimeAddressResultsTo:REMARKS·fliur1nf'1tuft.LV1niAWYJt-1:E.....ReceivedorLaboratoryby:'ObIOJ00-......-.J1'1'=p_TANALYSISTOBECOMPLETED:__CALLOUT__NORMALTURNAROUNDPERIOD__PRIORITY(NOCALLOUT)__CALLWITHRESULTSUPONCOMPLETION

## ENSR Page 000081 of 117 SAN



## APPENDIXB

Test Data

ENSR Fort Collins Environmental Toxicology Laboratory NELAC Accredited 

## 08503-132-265-006

Page B-1

## Page 000082 of 117 SAN Page 1 of

# ~~ FCETL QA Form No. 

### 051 Revision 4 Effective 

### 1/96


## TOXICITY DATA PACKAGE COVER SHEET 

# (§rrv/!JilJ?J9/ 

# oi;/of'

Test Type: Chronic Project Number: 

## 08503-132-265-006

Test Substance: Effluent Species: 

### Plmephale$ prometas

Dilution Water Type:**.....;.;.M;.:o~d..:..H;.:a:;.;rd=-------** 

### Organism Lot

## ~umber: 08[}/J l6

Concurrent Control Water Type: -...;..N;;.;./A...;.._

__ _ Age: "'- 

## 2 t..i hr:t< 24 

### hr) Supplier: FCETL**~;;...;;;..;~----** 

### Date and Time Test Began:

## ghsz'p~ @

## /~'J;,D
Protocol Number: USEPA 

### 2002 + Reg. VI

Background Information pH control?: Yes 

# @

If yes, give 

### % C0 2 : 

### Type ofTest: Static-Renewal

Test Temperature: 

### 25 

### ± 1 oc Env.

# Chmb@:~(p Test

# Chmbrs:~~~~~e!<!ia<!.!lk~er!..2s Test Solution Vol.: 

### 250m! Number of Replicates per Treatment:

___ 

## ....;5=-----
Length of Test: 7days Number of Organisms per Replicate:

___ ...;8;;._

__ _ 

### Type of Food and Quantity per Chamber: 

### 0.10 ml B.S. Feeding Frequency: 

### 3 x Daily

Test Substance Characterization Parameters and Frequency:
Hardness: Sx Receipt Alkalinity: Sx Receipt NHa: Sx Receipt TRC: Sx Receipt pH: Daily Conductivity: Daily 

# s-1-J'

Test Concentrations (Volume:Volume): MH, 

### 0.5, 

### ·1. ·5, 32% and 

# ¢o with CNT Effluent
Agency Summary Sheet(s)?: ..;..N;;;..A.;__

______ _

Reference Toxicant Data: Test

# D~tes: 

# tJf 

# rf o;; to 

# ¥'flr'/OX

Hlst. 95% Control Limits: 

# -=tfl1 to Method for Determining Ref. Tox. Value: Linear Interpolation

Special Procedures and Considerations: D.O. maintained<: 

### 4.0 mg/L •conductivity measured in dilution water and 

### 100% effluent at test termination

If survival In any test chamber falls below 50%, reduce feeding in that chamber to 0.05 ml of brine shrimp

I

J 

# J

. Study Director Initials:

# f'l-~ Date: 

# ?f(rr(D?

® 

# g (c;

.. 

## o.q <:t=' 

# {1j)UJJ 

# ?/1//)r e f...,f· "!- 1

## \v~

### Project Number:

0.5% 7



### 1% 15 

### 73 

## Page 000083 of 117 SAN

TEST SUBSTANCE USAGE LOG

08503-132-265-006 

### Page 2 of 

# .Jt_
FCETL QA Form No. 041 Revision 4 Effective 1/96

## IIProject Number·

Test· 

## %Cone. Replicate
MH A 

## 8 

# c D E
0.5% 

## A B


# c
D 

### E


## 1% A 

## B 

# c D E
5% A 

### B 

# c
D 

## E
32% A 

### B 

# c
D

## ~·CNT A 

# :YJ 8 

# c D 

## E
Date: 

## Time: Initials: Page 000084 

## of 

## 117 SAN

FATHEAD MINNOW (PIMEPHALES PROMELAS) CHRONIC BIOLOGICAL DATA Page 3 

## of 

# lf_ FCETL QA Form No. 060 Revision 2 Effective 

## 1/96

08503-132-265-006 

# II

Day



## 0 

# Day 1
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# 8 

# 6

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# r o Day 2 

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### Page 

## 000085 

## of 117 SAN

CHRONIC CHEMICAL DATA (INITIAL)


## Project Number: 08503-132-265-006

Test Species: Plmephales promelas



## o/o Day Day Day Day Day Day 

## 1 2 3 4 

## 5 6 Cone.: 

# B. I h. 

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# qo Alk. (mg/L)

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# fot TRC (mg/L) 

## t.O 

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## D.O. (mg/L)

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# flK IJ(yf PI( Day 

## 7



# ...... .cn'J Page 4 

## of 

# /f(
FCETL QA Form No. 058 Revision 

## 3 Effective 3/98

Meter# 

## Remarks

All Cone.

## f~o

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Note: Hardness, alkalinity, TRC and NH3 data appearing on this page have been transcribed from the wet chemistry log FCETL QA Form No. 084. *Dillutionfcontrol water and effluent were brought to 25"C prior to making the dillution series. The temperature of resulting effluent dilution_Jf**as~u~~d** 

### to also be 25"C.

# ~'b~~~ 

# '""'

### Page 

## 000086 of 117 SAN

CHRONIC CHEMICAL DATA (INITIAL)


### Project Number: 08503-132-265-006

Test Species: Plmephales promelas

% 

# I Day Day Day Day Day Day Day 

## 0 1 2 

## 3 4 5 6 Cone.: 11 

### 32 

## % I 

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# 7.1> 

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### Cone.: 

## ll100% 

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### Page 

## 5of/K FCETL QA Form No. 058 Revision 3 Effective 3/98

Meter# Remarks

All 

### Cone.

Note. Hardness, alkalinity, TRC, and NH3 data appeanng on th1s page have been transcnbed from the wet chem1stry log FCETL QA Form No. 084. *Dill uti on/control water and effluent were brought to 25"C prior to making the dillutlon series. The temperature of resulting effluent 

### dilytlop is assumed to also be 

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# II o.5% 

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# e.o 1.'1

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Date:

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Initials: 

# dKJ 

# c:¥ Page 000087 of 117 SAN

Day Day Day Day Day

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*conductivity



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## ({D~ 

# '1/t (j)X 1...-/.)

## Page 000088 of 

## 117 SAN

DAILY TOXICITY TEST LOG Project Number:**08503-132-265~006** 

## Test Species: Plmephales promelas



## General Comments

Test Day 0 Test Solution Mixed at: 

# /b/'i" Test Organisms Added at: 

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# l>?/Rs**~)zz_~~**5E6A4E1E2A4C5A3E1=ControlSIXTREATMENTSFIVEREPLICATESRANDOMCHAMBERLOCATION"Tau"Study#8503-132-265-0062E6050201C1A48186E6C582C283A68102=0.53=1%..efftttafit®4=55=326s-2.(f)Q),.L?J?It/D'%"GP@1)0_-fiK.C/1)01[-o&(Sf..303C3840.Page<6of/j_Ol!r:/07/ott5C4A""0!ruFro000,glc.oIQ.............-.....1(J))>z((/fFCETLQAFormNo.010Revision5Effective08/99fuv-plL.-..TESTORGANISMLENGTHS.WEIGHTS.ANDLOADING-'ProjectNumber:.TestSubstance:(!)lj)_""'"'"'-'4..\-Species:-.-Analyst<{:',-.JtAnalystGross:AfDate/TimeWt.:{22.(0&@i3"16DatemmeofGrossWt.:q1'1-J{o$8..\l3oBoatTreatmentRep.lengthWeightTyp:(Circle):·wetBlotDrvOr;AFDW(>SOO"C)No.Units:TareGrossNetWeightAdjustedNo.ofMeanWlperWeight(g)Weight(g){g)NetWeightOrig.Original(g)1'OrganismsOrganism.6)(mg)fVLK_A:-0(J11S>rrd.\at1Z:.o.nstg.___.--c_o.o.Cf-;lS)0-6oCJ07'Po.q31%o.o.063oo56>TEs1.f;.o.13b&6o.l}yII'/()-01>1.f31./(J.t;'o/..A-o.Cf3ZIIJIo.\1>0.()05"1-b-C--6·Do'{11l>o."':;lo-:;o.oo1-i1.-,[4.T€)E:.Q.9YIOIlfoil+o.q3.o.o03J6I'K0·00%1BlankRangeMeanTestSolutionVolume:LoadingRate:A-1..1:-......-!.-.1...&1.-•L._..(lftf'\b.J{-.'tlv'")1lt1W"e:;togComments:AnalyticalBalance10:S1r1l-IDriedinfromDate:Time:toDate:LotorQumber:Ckf)SoX'--)MeanWlperNo.ofMeanWt.MeanWlperTreatmentSurv.perTreatment(mg){mg)OrganismsSurviving(Surviving){Original)Organism{mg)q0gs'1>cgco'btf)-%--7'i.'I..j"'0CDIO000<.00(!,.;-..i.....lr._,_.......(f))>zhfwpll..-·ProiectNumber:·-l'52z.i.S'...Species:-._?.. 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(g)A0.933930.938480.00455B"l0.00000MHc-0.932480.937550.00507D0.931460.934460.00300E0.936800.941140.00434A0.932190.937170.00498B0.930480.935740.005260.5%c0.932490.936660.00417D0.933520.937030.00351E0.936570.941010.00444A0.930840.934200.00336B0.929460.934330.004871%c0.931670.935830.00416D0.933170.936600.00343E0.937500.941730.00423A0.932850.936450.00360B0.931380.934750.003375%c0.934210.938960.00475D0.934140.938810.00467E0.931430.934710.00328'"4A0.935940.939930.00399B0.937370.941790.00442c0.935360.939820.00446®D0.932960.937160.00420E0.936720.941490.00477(f)-·fI@,..6.rJ/t>eeSpecies:FatheadminnowMeanWe/Mean'Wt.JMean'Wt./Mean\Nt.JAdjustedOriginalTreatmentNumberofSurvivingTreatmentNetWeightNoofOrig.Organism(mg)Surv.Organism(mg)(g)Organisms(mg)(OriginaOOrganisms(mg)(Surviving)•0.0045580.5690.586280.5690.58620.000000--0-0.0050780.63480.6340.0030050.60050.6000.0043480.54380.5430.0049880.6220.571580.6220.58830.0052680.65880.6580.0041780.52180.5210.0035170.50160.5850.0044480.55580.5550.0033680.4200.501370.4800.52550.0048780.60980.6090.0041680.52080.5200.0034380.42970.4900.0042380.52980.5290.0036080.4500.503880.4500.519810.0033770.48160.562!0.004758 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## Page 000095 of 117 SAN

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

# Page 

# l!/. of/<{



# . 'f--":?JJ. g

# T;/ctl_~

# CIPr~· 

# ;Je"'i/1;7/:if:.
File: 265006po.dat Transform: NO TRANSFORMATION
Summary Statistics on Data TABLE 1 of 2

GRP IDENTIFICATION N 

### MIN MAX 

## MEAN

1 0 4 0.5430 0.6340 0.5865 2 0.5 5 0.5010 0.6580 0.5714 3 l 5 0.4200 0.6090 0.5014 4 

# S't, 5 5 o.4loo o.594o o.5o38 5

## ~% CNT 5 0.4990 0.5960 0.5460

## ------------------~--------------------------------------------------------

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original File: 265006po.dat Transform: NO TRANSFORMATION
Summary Statistics on Data TABLE 2 of 2

GRP IDENTIFICATION 

## VARIANCE SD 

## SEM 

# c.v. %

1 0 0.0015 0.0393 0.0197 6.7033 2 

### 0.5 0.0045 0.0668 0.0299 11.6828 3 1 0.0061 0.0783 0.0350 15.6257 4**')~** 

### 5 0.0067 0.0818 0.0366 16.2414 5 

# _0% CNT 0. 0013 0. 0365 0. 0163 6. 685.6 

# --------------QD------------------------------------------------------------

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original File: 265006po.dat Transform:

D = 

### 0.0791 

# w 

# = 0.9604

Critical 

# w w Shapiro

- Wilk•s Test for Normality

0.8840 (alpha 0.9160 (alpha 0.01 0.05 N 

# = 24) N 

### 24) NO TRANSFORMATION

## Page 000096 of 117 SAN

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original 

# Page!{of

# /~**f-~tJ Y/~/d(**  <l W; 

# lt'UK/'2i7 

# /cf

File: 265006po.dat Transform: NO TRANSFORMATION

Bartlett's Test for Homogeneity of variance

Calculated B1 statistic = 

### 3.4425 (p-value = 

### 0.4867)

Data PASS B1 homogeneity test at 0.01 level. Continue analysis.

Critical B 

# = 13.2767 9.4877 (alpha (alpha 

### = 0.011 

## df 

# = 0.05, df 4) 

### 4)



### Using Average Degrees of Freedom (Based on average replicate size of 4.80)

Calculated B2 statistic =



### Data 8B2 homogeneity 2.7543 (p-value = 

### 0.5997)

test at 0.01 level. Continue analysis.

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original File: 265006po.dat Transform: NO TRANSFORMATION

SOURCE DF

Between 4



### Within (Error) 19

Total

Critical F 23

= 

### 4.5003 2.8951 ANOVA Table

(alpha = 

### (alpha 

# ss

0.0278

0.0791

0.1069

0.01, df 0.05, df 4,19) 4,19) MS F



### 0.0069 1.6665

0.0042

(p-value = 

### 0.1992)

Since F < 

### Critical F FAIL TO REJECT Ho: All equal (alpha= 0.05)



# -------------------------------------------------------------------------------------··---

## Page 000097 of 117 SAN

Title: 8503-132-265-006 FC 8/15-22/08 dwt/original File: 265006po.dat Transform:

Bonferroni t-Test TABLE 1 OF 2

# Page~of/f

# ~;;.rJ**i/~:tlo~** {J.fr·. 

# fr{U)gjziJt(

NO TRANSFORMATION

Ho: Control<Treatment

TRANSFORMED MEAN MEAN CALCULATED IN SIG GROUP IDENTIFICATION ORIGINAL UNITS t STAT 0.05

1 0 

### 0. 5865 0. 5865 2 o.5 0.5714 0.5714 0.3488 

## I

4 3

## ~"'' 51 0. 5014 0. 5014 l. 

### 9658 

# J-)1(') (

J**~** 

### 0.5038 0.5038 1.9104

# --~------------~~~-=~=------~~=::~-------------~~=::~--------~~===~----- Bonferroni t critical value 

# = 2.4334 (1 Tailed, alpha= 

# o.os, df = 4,19)



### Title: 8503-132-265-006 FC 8/15-22/08 dwt/original File:

GROUP 265006po.dat Transform: NO TRANSFORMATION

Bonferroni t-Test

IDENTIFICATION TABLE 2 OF 2 

### Ho: Control<Treatment

NUM 

### OF

REPS MIN SIG DIFF % OF DIFFERENCE (IN ORIG. UNITS) CONTROL FROM CONTROL

l 0 4 

### 2 0. 5 5 0 .1053 18. 0 0. 0151 3 .,., 1 5 0.1053 18.0 0.0851 4 ..., ,.., 5 5 0.1053 18. 0 0. 0827 5

. 

# _>v.(% CNT 5 0. 1053 18. 0 0. 0405

# ---------------~-----------------------------------------------------------

### Cone. ID 1 

## Page 000098 of 117 SAN 

# r?;o :?-t**?"'2---~5-o** 

# oy,
2 

# Page 

# 11 of.{( 

# /1- 6f-.J rg /

# ~)o·rt a rr; fr{tfJ 

## g{ ')17 

# tor 3 4



### Cone. Tested 0 

### 0.5 1 

### 5


### Response 1 .569 .622 .42 .45 Response 2 .634 .658 .609 .481 Response 3 • 6 

### .521 .52 .594 Response 4 .543 .501 .429 .584 Response 5 .555 .529 .41 

# *** Inhibition Concentration Percentage Estimate 

# *** Toxicant/Effluent: effluent Test Start Date: 8/15/08 Test Ending Date: 8/22/08 Test Species: P. promelas Test Duration: 7 days DATA FILE: 265006go.icp OUTPUT FILE: 265006go.i25

Cone.



## ID

1 2 

### 3 4 5 

### Number· Replicates

4 5 5 

### 5 

### 5 Concentration %



### 0.000 0.500 1. 

### 000 5.000 32.000 Response Means

0.587 0.571 0.501 0.504 0.000 Std. Dev,

0.039 0.067 0.078 0.082 

# o.ooo 5



### 32

0 0 0 

### 0 0

Pooled Response Means

0.587 0.571 0.503 0.503 0.000

The Linear Interpolation Estimate: 8.3696 Entered P Value: 25

Number of Resamplings: 80 The Bootstrap Estimates Mean: Original Confidence Limits: Expanded confidence Limits: Resampling time in Seconds: 7.3416 Standard Deviation: 1.3265 Lower: 4.4158 Upper: 10.0959 Lower: 2.4389 Upper: 10.9590 0.00 Random_seed: -334101559

## Page 000099 of 117 SAN 

# Page: 

# I g

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# 1i FCETL QA Form No. 15-

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# ~ltr/o8 SUBJECT: DAILY LOG

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### Page 000100 of 117 SAN



# 10e Structure of Carbon Nanotubes

Single wall carbon nanotubes (SWNT) are an allotrope of sp 2 

### hybridized carbon similar to fullerenes. The
structure can be considered as a cylindrical tube comprised of 6-membered carbon ring as in graphite. The cylindrical nanotubes usually have at least one end capped with a hemisphere of the buckyball or fullerene structure. Typical structures are shown below:



## m.m.•·**f~Hl1**

### Page 000101 of 117 SAN

The detailed structure of the nanotubes varies with diameter and is defined by the chiral angle as shown in the diagram below. The distance from the origin defines the diameter of the tube.

Carbon nanotubes produced by SouthWest Nanotechnologies have the following typical properties:

Parameter CG 100 SG 65 SG76

Tube Diameter (nm) from OA and 1.0 

## ± 0.3 0.8 

## ± 0.1 0.9 

## ± 0.2 AFM Tube Length (nm) from AFM Mode 800 900 Not Measured Range 400- 2,300 400- 2,500 Carbon Content{%) >90 >90 >90 SWNT Content {% carbon as 75 80 80 SWNT) Dominant Chiralities {6,5), (7,5), (6,5) (7,6) (7,6)



# Chirality and Tube Diameter
The chirality of the carbon nanotube dictates its diameter. In the manufacturing of carbon nanotubes many different chiralities of carbon nanotubes are formed. With the CoMoCat® process it is possible to vary the reactor conditions to manufacture products with dominant chiralities. Various techniques are

### Page 000102 of 117 SAN

available to determine the chirality of the tubes present. The dominant chiralities for each grade are shown in the table above. The most common techniques are:
a) Optical Absorption measurements in the UV-visible-Near IR region of the spectrum b) Raman Spectroscopy in the region ofthe radial breathing mode of the spectrum c) Fluorescence spectroscopy.
Optical absorption measurements are used routinely in the manufacturing operation to monitor the chirality of the product produced. Typical Spectra for the three grades of material currently produced

are shown below. The main chiralities can be determined from the peaks caused by the Van Hove transitions in the Density of States and are marked on the Spectra.

Typical Optical Absorbance Spectrum fot**SWeN~** SG 65

2.5 

## E'
c 0**~** 2.0 

## 2l c 

# "' -e 0 1.5 rJ) .0 

### <t: "0

## -~ "iii 1.0 

## E 0 

### z 0.5

400 (6,5)

600 (6,5)

(7,6)

(7,6)

800 1000 1200 1400 Wavelength (nm)



### The Optical absorbance spectrum for SG65 is dominated by the large peak associated with (6,5) chirality tubes and a smaller peak for the (7,6) tubes. As can be seen from the spectrum the peaks show in two distinct regions of the spectrum. These two regions correspond to two different electronic transitions known as 511, (850- 1400 nm) and 522, (550- 750 nm). Tubes are identified by their transitions in both regions of the spectrum from published tables, (see attachment.) Once the chiralities have been established the diameter can also be determined from published tables: The nominal diameter of the (6,5) tube is 0.857 nm and that of the (7,6) tube 0.895 nm thereby giving an average diameter of 0.8±0.1

nm.2.0 

## 'E 1.8 c 0 00 

### !::::-
Ql 1.6 (.) c rn 

# -e 1.4 0 Ill .c <( "0 1.2 Ql .!::! 

### a; 

## E 1.0 0 

### z

0.8 

### Page 000103 of 117 SAN

Typical Optical Absorbance Spectrum fot**SWeN~** SG 76

400 600 (8,7) [1,6) (8,6)

800 1000 Wavelength (nm) [1,6)

1200 1400



### Similarly for the SG76 material the dominant chiralities are for (7,6), (8,6) and (8,7) tubes. From tables the diameters of these tubes are 0.895, 0.966 and 1.032 nm respectively.

Typical Optical Absorbance Spectrum fot**SWeN~** CG-100

2.5 

## 'E
c (6,5) 0 00 2.0 

### !::::- Ql (.) [1,5) c rn [1,6) 

# -e 0 1.5 Ill .c <( "0 Ql .!::! 1.0 (8,7) 

### a; E 

### 0 z 0.5

400 600 800 1000 1200 1400 Wavelength (nm)



### CG 100 differs from the other materials in that it has a wider range of chiralities and hence diameters. Based on the OA spectrum the diameter of the tubes has been determined to be in the range of 1.0±0.3

nm.

### Page 000104 of 117 SAN



## Tube Length
Tube length has been measured directly using an Atomic Force Microscope. For SG 65 material the mode (most common frequency) of the distribution is approximately 900 nm with a range from approximately 400 10 2500 nm, with >95% ofthe tubes falling within the range Of 500 to 2,000 nm.



# Length Distribution SG 65

3 

# a 

# !

0 

# B :!a 

# 5 z

1 

# a

a

### Page 000105 of 117 SAN

The length of CG 100 tubes was measured by the 'same method.



# CG Length distribution

IIICG 191
Total Tubes > 250 20"



# II.

...**##~#~~,~~'''~''''** LerQ:h(nm)



### The most common length frequency was measured as 800nm with a typical range from 400 to 2,300

nm. Two outliers were observed at approximately 3 and 3.5 !J.m.

The length of SG76 tubes has not been determined, but is expected to be similar to SG 65 and CG 100.

### Page 000106 of 

### 117 SAN



# Carbon and SWNT Content Purity of the material is determined by Thermogravimetric Analysis. In this process the material is heated in a furnace at a rate of 5 C degrees/minute while the sample weight is continuously monitored.

An example TGA is shown below. The green curve represents the loss in weight of the sample as it is heated while the blue curve is the derivative of the weight loss.

Sample: SG6S.000..0010 Run Date: 2()08..06.14 09:17

### 100-~----------------------------------------------------------r 1.2

.**··~···-** ---..... 149.83'C' 98.46% 

### T1SS2.46

Resldufil messa4.829 

### 1.0



# 6 " 

# -**~** 60-i 

### l: .21

# ~**.~** 

### .... 0.4 

## (3
40

(I 

## :w 

## j \ 

## "

____**__.~ ~----~---**  

### Catalyst oxides and carbides

;/

340 540 

### Temperature ("C)**-~"""""~----·** 

### 0.0 748.02'C 3.913%**~-----------··+-··'-** 

# ---.---·---····---·-·-+

640 

### 740



### From the TGA it is possible to estimate the proportion of single wall nanotubes, other forms of graphitic carbon and residual catalyst

• The first derivative peak is a result of the oxidation of single wall carbon nanotubes. Tl% is representative of the proportion of single wall nanotubes in the material

• The second derivative peak above T1 represents the oxidation of other forms of graphitic carbon formed as a by-product in the manufacturing process.

• The residual mass at 

### 62s•c is representative of the non carbonaceous material, primarily metal oxides and carbides from the catalyst.

### Page 000107 of 117 SAN

Thus from the TGA we can determine the total carbon content, the percentage of carbon which is comprised of single wall carbon nanotubes and the residual mass.


# Additional Structural Data
As mentioned above, Raman Spectroscopy can also be used to determine the distribution of chiralities in the material. This is a very complicated procedure and to date has only been conducted on early SG 65 type material. This work was conducted independently at Universidade Federal de Minas Gerais, Belo Horizonte, Brazil by Jorio, A et. a/ published in Physical Review B 72, 075207 {2005.} 

### The Raman study confirmed the dominant peaks seen in the OA spectrum for SG 65 plus a high proportion of (7,5) tubes. The (7,5) tube would be seen as a shoulder on the high wavelength side of the (6,5) peak. The (6,5) Optical Absorbance Spectrum shown earlier does not show this peak. This is due to tighter control of manufacturing conditions for this material, which has significantly reduced the presence of this particular tube.

A total of 35 different chiralities were identified.

The three grades of material have also been characterized by Transmission Electron Microscopy, (TEM.) Typical TEMs for the three grade SG 65, SG 76 and CG 199 are shown below:



## Typical TEM Image for SWeNT® SG 65

### Page 000108 of 117 SAN



## Typical TEM Image for SWeNT® SG 76

Typical TEM Image for SWeNT® CG-100

### Page 0001 09 of 117 SAN

These TEM images show the strong tendency for nanotubes to form into bundles (or ropes.) The degree of roping is highly dependant on the conditions. For example some surfactants and polymers such as Sodium cholate or sodium dodecyl sulphate tend to break up bundles, whilst other surfactants, such as Triton X-100 tend to preserve the rope structure. Ropes may vary in diameter from 2 to 3 nm upto 20
to 

## 30 nm.

### Page 

### 000110 of 

### 11z7 SAN

SECTION 1 PRODUCT /DENT/FICA TION 

# Material Safety Data Sheet SWeNT® Freeze*Dried Powder Single-Wall €arbon Nanotubes

PRODUCT NAME: Carbon Nanotube Freeze-Dried Powder

- SG65, SG7 6, CG 1 00, CG200

OTHJ:R/GENERIC NAMES: SWNT; SWCNT, Single-Wall Carbon Nanotubes

MANUFACTURER: SouthWest NanoTechnologies, Inc. 2501 Technology Place Norman, OK 73071 -11 02 +1 405.217.8388- Tel +1 405.217.8389- Fax http://swentngno.com safety@swentnano.com

SECTION 2 COMPOSITION AND INFORMATION ON INGREDIENTS

Ingredient Name Single-Wall Carbon Nanotubes

Metallic impurities (including Molybdenum and Cobalt) CAS Number

None

Various Weight 

### o/o

85-99

1-15

Trace impurities and additional material names not listed above may also appear in Section 15 towards the end of the MSDS. These materials may be listed for local "Right- To-Know" compliance and for other reasons.

This material is considered as hazardous under OSHA regulations.

SECTION 3 HAZARDS IDENTIFICATION

EMERGENCY OVERVIEW: Product is a freeze-dried po'wder. May cause eye, skin and respiratory tract irritation. The complete physical and toxicological properties of this material have not been fully evaluated.

Potential Health Hazards

SKIN: This material may cause skin irritation. Use proper precautions.

EYES: This material may cause eye irritation. Use proper precautions.

INHALATION: This material may cause irritation to the mucous membranes and upper respiratory tract. The product presents an increased inhalation hazard because of the small particle size. Use proper precautions.

INGESTION: Not a probable route of exposure. This material may be harmful if swallowed.

DELAYED EFFECTS: None known.
Ingredients found on one of the OSHA designated carcinogen lists are listed below.

INGREDIENT NAME Cobalt Compounds NTP STATUS IARC STATUS 

### 2B OSHA LIST

## Page 000111 of 117 SAN

SECTION 

### 4 FIRST AID MEASURES

SKIN: Wash with soap and water. Get medical attention if irritation develops or persists.

EYES: Flush eyes with plenty of water. Get medical attention if irritation develops or persists.

INHALATION: Remove to fresh air. If not breathing, give artificial respiration. Get medical attention if irritation develops
or persists.

INGESTION: If person is conscious, rinse mouth with water. Do not induce vomiting unless directed to do so by a physician.

ADVICE TO PHYSICIAN: No specific advice, treat symptomatically.

SECTION 

### 5 FIRE FIGHTING MEASURES

FLASH POINT: Not determined. FLASH POINT METHOD: Not applicable. AUTOIGNITION TEMPERATURE: Not determined. UPPER FLAME LIMIT (volume 

## o/o in air): Not applicable. LOWER FLAME LIMIT (volume 

### % in air): Not applicable. FLAME PROPAGATION RATE (solids): Not determined. OSHA FLAMMABILITY CLASS: Not determined. DECOMPOSITION PRODUCTS.: Carbon Monoxide, Carbon Dioxide.

UNUSUAL FIRE AND EXPLOSION HAZARDS: Airborne dust from the dried dispersion in an enclosed space and in the presence of an ignition source may constitute an explosion hazard.

SPECIAL FIRE FIGHTING PRECAUTIONS/INSTRUCTIONS: As in any fire, wear NIOSH/MSHA approved positive pressure self-contained breathing apparatus and full protective clothing.

SECTION 6 ACCIDENTAL RELEASE MEASURES

IN CASE OF SPILL OR OTHER RELEASE: Use appropriate personal protection during clean up. Remove mechanically by a method that minimizes the generation of airbourne dust (HEPA equipped vacuum, wet mopping etc.) Abosrb material and place in appropriate containers for disposal. Do not allow spilled material or wash water to enter sewers, surface water, or ground water.
SpiUs and releases may have to be reported to Federal and/or local authorities. See Section 15 regarding reporting requirements.

SECTION 

### 7 HANDLING AND STORAGE

NORMAL HANDLING: (Always wear recommended personal protective equipment.) Use local exhaust or general room/dilution ventilation sufficient to maintain exposure below permissible exposure limits (29 CFR 191 0.1 001 for asbestos). If possible, use in a closed well ventilated area (e.g. fume hood).

STORAGE RECOMMENDATIONS: To maintain product quality, store between 40 and 100 °F.

## Page 000112 of 117 SAN

SECTION 

### 8 EXPOSURE CONTROLS AND PERSONAL PROTECTION



### ENGINEERING CONTROLS: General- room ventilation is adequate for storage and ordinary handling. Use local exhaust at points of use to maintain exposure below the PEL/TL V exposure limits.

PERSONAL PROTECTIVE EQUIPMENT

SKIN PROTECTION: Impervious garments and gloves.

EYE PROTECTION: Wear chemical goggles that conform to ANSI Z87.1 under normal conditions.

RESPIRATORY PROTECTION: 

### If there is potential for inhalation of dust, vapors, or aerosols wear a full-face NIOSH approved respirator with N 1 00 cartridges or better.

The respirator must be selected based on contamination levels and use conditions found in the workplace. Use conditions must not exceed the working limits of the respirator. The respirator must be used in accordance with the OSHA respiratory protection standard (29 CFR 1 91 0.1 34).

ADDITIONAL RECOMMENDATIONS: Provide safety showers and eyewash stations in close proximity to the work area.

EXPOSURE GUIDELINES

INGREDIENT NAME ACGIH TLV Single-Wall Carbon Nanotubes Not available. OSHA PEL Not available. OTHER LIMIT None.

Insoluble Molybdenum Compounds, as Mo TWA= 10 mg/m3 (8- hr day, inhalable fraction), TWA= 3 mg/m3 (8-hr day, respirable fraction) Cobalt Compounds, as Co TWA= 0.02 mg/m3



# * ** *** = Limit established by SWeNT for internal use.

= Workplace Environmental Exposure Level (AIHA).

= Biological Exposure Index (ACGIH). (8-hr day) TWA= 0.05 mg/m3 (8-hr day) None.

15 ug/L urine 

# *** 1 ug/L blood 

# ***

PEL values represent limits established by the 1989 Air Contaminants Rule (29 CFR 1910.1000, Subpart Z, Table Z-1-A) which was subsequently revoked on June 30, 1993. Several states continue to enforce Table Z-1-A limits.
OTHER EXPOSURE LIMITS FOR POTENTIAL DECOMPOSITION PRODUCTS: None.

SECTION 

### 9 PHYSICAL AND CHEMICAL PROPERTIES 

### APPEARANCE: Black powder. PHYSICAL STATE: Solid. ODOR: None. SPECIFIC GRAVITY (water = 1.0): SOLUBILITY IN WATER (weight%): pH: 4-10 BOILING POINT: Not determined. MELTING POINT: Not applicable. VAPOR PRESSURE: Not applicable. Not determined. Insoluble.

VAPOR DENSITY (air= 1.0): Not determined. EVAPORATION RATE: Not determined. % VOLATILES: Not determined. FLASH POINT: Not determined. COMPARED TO:

(Flash point method and additional flammability data are found in Section 5.) Not applicable.SECTION 1 0 STABILITY AND REACTIVITY

STABILITY {CONDITIONS TO AVOID) Normally stable.

INCOMPATIBILITIES: None known.

HAZARDOUS DECOMPOSITION PRODUCTS: 

## Page 000113 of 117 SAN

Thermal decomposition products may include carbon monoxide, carbon dioxide and oxides of metallic impurities (including molybdenum and cobalt).

HAZARDOUS POLYMERIZATION: 

### Will not occur.

SECTION 

### 11 TOXICOLOGICAL INFORMATION

IMMEDIATE {ACUTE) EFFECTS: No data available.

DELA YEO {SUBCHRONIC AND CHRONIC) EFFECTS: No data available.

OTHER DATA: None.

SECTION 

### 12 ENVIRONMENTAL INFORMATION No data available.

SECTION 

### 13 DISPOSAL CONSIDERATIONS

Is the unused product a RCRA hazardous waste if discarded? No. If yes, the RCRA ID number is: Not applicable.

OTHER DISPOSAL CONSIDERATIONS: Disposal of this product is not allowed by federal, state and local government regulations. It must be destroyed by a suitable method including, but not limited to, incineration.

The information offered here is for the product as shipped. Use and/or alterations to the product, such as mixing with other materials, may significantly change the characteristics of the material and alter the RCRA classification and the proper disposal method. SECTION 14 TRANSPORTATION INFORMATION

US DOT HAZARD CLASS: Not regulated.

US DOT ID NUMBER: Not applicable. For additional information on shipping regulations affecting this material, contact the information number found on Section 1.

SECTION 15 REGULATORY INFORMATION TOXIC SUBSTANCES CONTROL ACT {TSCA)

TSCA INVENTORY STATUS: This material is manufactured under the terms of the TSCA LOREX exemption at 40 CFR 723.50(c)(2), and under the requirements of 40 CFR723.50U). Further, according to 723.50(k)(2) this material cannot be further distributed until it has been reacted, incorporated into an article, or otherwise rendered into a physical form or state.

OTHER TSCA ISSUES: None.

SARA TITLE 111/CERCLA "Reportable Quantities" (RQs) and/or "Threshold Planning Quantities" (TPQs) exist for the following ingredients.

INGREDIENT NAME SARA/CERCLA RQ (lb) SARA EHS TPQ (lb) No ingredients listed in this section.
Spills resulting in the loss of any ingredient at or above its RQ requires immediate notification to the National Response Center (1-800-424-8802) and to your Local Emergency Planning Committee.



# ------------------------------------------------------------------------------------------------------SECTION 311 HAZARD CLASS: 

## Page 000114 of 117 SAN

Immediate (Acute).

The following. ingredients are SARA 313 "Toxic Chemicals". CAS numbers and weight percents are found in Section 2.

INGREDIENT NAME Cobalt Compounds

STATE RIGHT-TO-KNOW COMMENT Elemental metal content< 0.1 0%.

In addition to the ingredients found in Section 2, the following are listed for state right-to-know purposes.

INGREDIENT NAME WEIGHT 

### % COMMENT No ingredients listed in this section.

ADDITIONAL REGULATORY INFORMATION

WHMIS CLASSIFICATION (CANADA): Not determined.

FOREIGN INVENTORY STATUS: All components of this product are listed on the following inventories:
Australian (AICS) Canadian (DSL) Chinese (IECSC) European (EINECS) Japanese (ENCS) Korean (KECI)

SECTION 16 OTHER INFORMATION CURRENT ISSUE DATE: PREVIOUS ISSUE DATE: August 1 0, 2009 April 22, 2009

CHANGES TO MSDS FROM PREVIOUS ISSUE DATE ARE DUE TO THE FOLLOWING: Additional product names were added.

OTHER INFORMATION: None.

DISCLAIMER: THE INFORMATION HEREIN IS PROVIDED IN GOOD FAITH, AND IS BELIEVED TO BE ACCURATE. NO WARRANTY, EXPRESSED OR IMPLIED, IS MADE REGARDING THE ACCURACY OR COMPLETENESS OF THIS INFORMATION. FURTHERMORE, SWENT MAKES NO REPRESENTATION OR WARRANTY THAT THE SAMPLE IS FIT FOR ANY PARTICULAR PURPOSE AND SWENT EXPRESSLY DISCLAIMS ANY WARRANTY OF MERCHANTABILITY. THIS DOCUMENT IS INTENDED ONLY AS A PRECAUTIONARY GUIDE FOR THE APPROPRIATE HANDLING OF THE MATERIAL BY A PROPERLY TRAINED PERSON USING THIS PRODUCT, WHO MUST EXERCISE INDEPENDENT JUDGMENT IN DETERMINING HANDLING OF THIS PRODUCT AND THE APPROPRIATENESS OF THIS PRODUCT FOR ANY PURPOSE. SWENT SHALL NOT BE LIABLE FOR CONSEQUENTIAL DAMAGES OR FOR DAMAGE TO PERSONS OR PROPERTY RESULTING FROM ITS USE. NOTHING HEREIN SHALL BE CONSTRUED AS A RECOMMENDATION FOR USE IN VIOLATION OF ANY PATENT. SWENT® IS A TRADEMARK OF SOUTHWEST NANOTECHNOLOGIES, INC. 

### CAUTION: POTENTIAL HAZARDS OF THIS PRODUCT ARE UNKNOWN. MANUFACTURED UNDER U.S. PATENT NOS. 6,333,016, 6,413,487, 6,955,800 AS WELL AS OTHER PENDING PATENT APPLICATIONS IN THE U.S. AND AROUND THE WORLD.Page0Si-ngle-WallCarb,onNanotubesManufacturedunderU.S.PatentNos.f)and695580_ctsweIIasotherpendlngpatentapplicationsIntheU.S.anda(ouhdtheworld.CAUTION:Potentialhazards'ofthisproductare:Unknown.Thisproductcanonlybe_usedunderTSCAR&Dconditlons.ComplianceWiththeenclosedMSD,Sisadvised.

## Page 000116 of 117 SAN

# ~ State of Ohio Environmental Protection Agency

STREET ADDRESS: 

### Lazarus Government Center 122 S. Front Street Columbus, Ohio 

### 43215


## March 

# 23, 2005

CERTIFIED MAIL

Mr. Fred Sigg Von Roll America, Inc.;· 

# 1250 Saint George Street East LiverpooJ,. Qhio 

# 43920



# ... Dear Mr. Sigg: TELE: (614) 644-3020 FAX: (614) 644-3184

· Yiww.epa.stata.oh.us MAILING ADDRESS: 

### ·P.O. Box 1049 Columbus, OH 43216-1049



## Re: Ohio Hazardous Waste Permit Renewal
vo·n Roll America, Inc. U.S. EPA 10 No.: OHD 

# 980 613 541 Ohio 10 No.: 

# 02-15-0589

Here is the renewed Ohio Hazardous Waste Facility Installation and Operation Permit (Permit) for Von Roll America, ·Inc. I have also enclosed a copy of the responsiveness summary Ohio EPA prepared in response to written comments the Agency received concerning the Part 8 permit application. The Permit is effective today, March 

# 23, 2005. The date-stamped, page-numbered copy of the Part B permit application is also enclosed.

Please remember that according to Rule 

# 37 45-50-36 of the Ohio Administrative Code your annual hazardous waste permit fee of 

## ·.j , ·::' :· will be due on March 

# 23,, 2006. Ohio EPA will try to notify you before this fee is due, but it is your responsibility to make

.... sure it gets paid on time.

As a party to this permit proceeding, you may appeal this Permit to the Environmental Review Appeals Commission (ERAC) no later than 

# 30 days after the public notice (See Ohio Revised Code§ 

# 3745.04). You may file your appeal with ERAC at the following address: Environmental Review Appeals Commission, 

# 309 South Fourth Street, Room 

# 222, Columbus, Ohio 

# 43215.

If you file an appeal, you must put itin writing. Your appeal must explain why you are appealing the action and the grounds you are using for your appeal. You must send a copy of the appeal to the director of the Ohio Environmental Protection. Agency no later than three (3) days after you file it with ERAC.



## @**Print~donRecycledPaper** Bob Taft, Governor Bruce Johnson, Lieutenant Governor Joseph P. Konceiik, Director


### Ohio EPA Is an Equal Opportunity Employer

## Mr. Fred Sigg 

# 1 Von Roll America, Inc. March 23, 2005 Page Two Page 000117 of 117 SAN

If you have any questions concerning compliance, do not hesitate to call Patricia Natali of Ohio EPA's Northeast District Office at (330) 385-8447.
Sincerely,

# ~**~** Pamela S. Allen, Manager Regulatory and Information Services Division of Hazardous Waste Management

Attachments

cc: Edwin Lim, Mgr., ERJ}.S, DHWM Jeremy Carroll/John Nyers, ERAS, DHWM Harriet Croke, US EPA, Region V Frank Popotnik/Patricia Natali, DHWM, NEDO Mike Settles, Public Interest Center, Ohio EPA file

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